LOCUS       DADWTH010000001        85895 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.001, whole genome shotgun sequence.
ACCESSION   DADWTH010000001 DADWTH010000000
VERSION     DADWTH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.001"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1592)
                     /locus_tag="JNY02_000001"
     CDS             complement(<1..1592)
                     /locus_tag="JNY02_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB9431696.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDSSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="JNY02_000002"
     CDS             complement(1576..1932)
                     /locus_tag="JNY02_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBB9431697.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSTKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2055..2237)
                     /locus_tag="JNY02_000003"
     CDS             complement(2055..2237)
                     /locus_tag="JNY02_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431698.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="JNY02_000004"
     CDS             complement(2221..2661)
                     /locus_tag="JNY02_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB9431699.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="JNY02_000005"
     CDS             complement(2661..2957)
                     /locus_tag="JNY02_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBB9431700.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="JNY02_000006"
     CDS             complement(2954..3292)
                     /locus_tag="JNY02_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB9431701.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="JNY02_000007"
     CDS             complement(3289..4500)
                     /locus_tag="JNY02_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB9431702.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="JNY02_000008"
     CDS             complement(4502..5074)
                     /locus_tag="JNY02_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBB9431703.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="JNY02_000009"
     CDS             complement(5114..6271)
                     /locus_tag="JNY02_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB9431704.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGNTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6574..6735)
                     /locus_tag="JNY02_000010"
     CDS             complement(6574..6735)
                     /locus_tag="JNY02_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBB9431705.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(6745..7050)
                     /locus_tag="JNY02_000011"
     CDS             complement(6745..7050)
                     /locus_tag="JNY02_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431706.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7047..7325)
                     /locus_tag="JNY02_000012"
     CDS             complement(7047..7325)
                     /locus_tag="JNY02_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431707.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7605..9353)
                     /locus_tag="JNY02_000013"
     CDS             complement(7605..9353)
                     /locus_tag="JNY02_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBB9431708.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9350..9649)
                     /locus_tag="JNY02_000014"
     CDS             complement(9350..9649)
                     /locus_tag="JNY02_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBB9431709.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(9655..9888)
                     /locus_tag="JNY02_000015"
     CDS             complement(9655..9888)
                     /locus_tag="JNY02_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431710.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(9890..10110)
                     /locus_tag="JNY02_000016"
                     /pseudo
     CDS             complement(9890..10110)
                     /locus_tag="JNY02_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10103..10324)
                     /locus_tag="JNY02_000017"
     CDS             complement(10103..10324)
                     /locus_tag="JNY02_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431711.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10314..10514)
                     /locus_tag="JNY02_000018"
     CDS             complement(10314..10514)
                     /locus_tag="JNY02_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBB9431712.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10523..11269)
                     /locus_tag="JNY02_000019"
     CDS             complement(10523..11269)
                     /locus_tag="JNY02_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431713.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11327..11515)
                     /locus_tag="JNY02_000020"
     CDS             complement(11327..11515)
                     /locus_tag="JNY02_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBB9431714.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(11890..13119)
                     /locus_tag="JNY02_000021"
     CDS             complement(11890..13119)
                     /locus_tag="JNY02_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB9431715.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13368..14489
                     /gene="roxA"
                     /locus_tag="JNY02_000022"
                     /gene_synonym="ycfD"
     CDS             13368..14489
                     /gene="roxA"
                     /locus_tag="JNY02_000022"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBB9431716.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14538..15764)
                     /gene="pepT"
                     /locus_tag="JNY02_000023"
     CDS             complement(14538..15764)
                     /gene="pepT"
                     /locus_tag="JNY02_000023"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBB9431717.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16014..17150
                     /gene="potA"
                     /locus_tag="JNY02_000024"
     CDS             16014..17150
                     /gene="potA"
                     /locus_tag="JNY02_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBB9431718.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17134..17991
                     /gene="potB"
                     /locus_tag="JNY02_000025"
     CDS             17134..17991
                     /gene="potB"
                     /locus_tag="JNY02_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBB9431719.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            17988..18782
                     /gene="potC"
                     /locus_tag="JNY02_000026"
     CDS             17988..18782
                     /gene="potC"
                     /locus_tag="JNY02_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBB9431720.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            18779..19825
                     /gene="potD"
                     /locus_tag="JNY02_000027"
     CDS             18779..19825
                     /gene="potD"
                     /locus_tag="JNY02_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBB9431721.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSQLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(19981..20802)
                     /gene="cobB"
                     /locus_tag="JNY02_000028"
     CDS             complement(19981..20802)
                     /gene="cobB"
                     /locus_tag="JNY02_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBB9431722.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(20818..21729)
                     /gene="nagK"
                     /locus_tag="JNY02_000029"
     CDS             complement(20818..21729)
                     /gene="nagK"
                     /locus_tag="JNY02_000029"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBB9431723.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(21758..23002)
                     /gene="lolE"
                     /locus_tag="JNY02_000030"
     CDS             complement(21758..23002)
                     /gene="lolE"
                     /locus_tag="JNY02_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBB9431724.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23002..23703)
                     /gene="lolD"
                     /locus_tag="JNY02_000031"
     CDS             complement(23002..23703)
                     /gene="lolD"
                     /locus_tag="JNY02_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBB9431725.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVTMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(23696..24895)
                     /gene="lolC"
                     /locus_tag="JNY02_000032"
     CDS             complement(23696..24895)
                     /gene="lolC"
                     /locus_tag="JNY02_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBB9431726.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25157..26203
                     /locus_tag="JNY02_000033"
     CDS             25157..26203
                     /locus_tag="JNY02_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBB9431727.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSTVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26331..29777
                     /gene="mfd"
                     /locus_tag="JNY02_000034"
     CDS             26331..29777
                     /gene="mfd"
                     /locus_tag="JNY02_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBB9431728.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     SERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            29920..30882
                     /gene="ldtC"
                     /locus_tag="JNY02_000035"
                     /pseudo
     CDS             29920..30882
                     /gene="ldtC"
                     /locus_tag="JNY02_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(30965..31222)
                     /gene="bhsA"
                     /locus_tag="JNY02_000036"
     CDS             complement(30965..31222)
                     /gene="bhsA"
                     /locus_tag="JNY02_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBB9431729.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31463..32095
                     /gene="comR"
                     /locus_tag="JNY02_000037"
     CDS             31463..32095
                     /gene="comR"
                     /locus_tag="JNY02_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBB9431730.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32157..32696)
                     /locus_tag="JNY02_000038"
     CDS             complement(32157..32696)
                     /locus_tag="JNY02_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBB9431731.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(32923..34227)
                     /gene="ndh"
                     /locus_tag="JNY02_000039"
     CDS             complement(32923..34227)
                     /gene="ndh"
                     /locus_tag="JNY02_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBB9431732.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34628..35170)
                     /locus_tag="JNY02_000040"
     CDS             complement(34628..35170)
                     /locus_tag="JNY02_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB9431733.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35193..36218)
                     /gene="nagZ"
                     /locus_tag="JNY02_000041"
     CDS             complement(35193..36218)
                     /gene="nagZ"
                     /locus_tag="JNY02_000041"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBB9431734.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36229..37053)
                     /gene="thiK"
                     /locus_tag="JNY02_000042"
     CDS             complement(36229..37053)
                     /gene="thiK"
                     /locus_tag="JNY02_000042"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBB9431735.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37034..37675)
                     /gene="lpoB"
                     /locus_tag="JNY02_000043"
     CDS             complement(37034..37675)
                     /gene="lpoB"
                     /locus_tag="JNY02_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBB9431736.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(37690..38067)
                     /locus_tag="JNY02_000044"
     CDS             complement(37690..38067)
                     /locus_tag="JNY02_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBB9431737.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38070..38429)
                     /gene="hinT"
                     /locus_tag="JNY02_000045"
     CDS             complement(38070..38429)
                     /gene="hinT"
                     /locus_tag="JNY02_000045"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBB9431738.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            38763..39587
                     /locus_tag="JNY02_000046"
     CDS             38763..39587
                     /locus_tag="JNY02_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBB9431739.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            39591..40952
                     /gene="fhuE"
                     /locus_tag="JNY02_000047"
                     /pseudo
     CDS             39591..40952
                     /gene="fhuE"
                     /locus_tag="JNY02_000047"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41012..42445)
                     /gene="ptsG"
                     /locus_tag="JNY02_000048"
     CDS             complement(41012..42445)
                     /gene="ptsG"
                     /locus_tag="JNY02_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBB9431740.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(42740..43537)
                     /locus_tag="JNY02_000049"
     CDS             complement(42740..43537)
                     /locus_tag="JNY02_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBB9431741.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43548..44552)
                     /gene="holB"
                     /locus_tag="JNY02_000050"
     CDS             complement(43548..44552)
                     /gene="holB"
                     /locus_tag="JNY02_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBB9431742.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44549..46201)
                     /gene="yceG"
                     /locus_tag="JNY02_000051"
     CDS             complement(44549..46201)
                     /gene="yceG"
                     /locus_tag="JNY02_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBB9431743.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKMRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGTQLAEKLRSLVL
                     DIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHDLSTQAYQGGGR
                     GIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIEQESFDFFNRTR
                     ARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(46204..47013)
                     /gene="pabC"
                     /locus_tag="JNY02_000052"
     CDS             complement(46204..47013)
                     /gene="pabC"
                     /locus_tag="JNY02_000052"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBB9431744.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47133..48374)
                     /gene="fabF"
                     /locus_tag="JNY02_000053"
     CDS             complement(47133..48374)
                     /gene="fabF"
                     /locus_tag="JNY02_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBB9431745.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48461..48697)
                     /gene="acpP"
                     /locus_tag="JNY02_000054"
     CDS             complement(48461..48697)
                     /gene="acpP"
                     /locus_tag="JNY02_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB9431746.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(48908..49642)
                     /gene="fabG"
                     /locus_tag="JNY02_000055"
     CDS             complement(48908..49642)
                     /gene="fabG"
                     /locus_tag="JNY02_000055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBB9431747.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(49655..50584)
                     /gene="fabD"
                     /locus_tag="JNY02_000056"
     CDS             complement(49655..50584)
                     /gene="fabD"
                     /locus_tag="JNY02_000056"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBB9431748.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50600..51553)
                     /gene="fabH"
                     /locus_tag="JNY02_000057"
     CDS             complement(50600..51553)
                     /gene="fabH"
                     /locus_tag="JNY02_000057"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBB9431749.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLGRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51621..52691)
                     /gene="plsX"
                     /locus_tag="JNY02_000058"
     CDS             complement(51621..52691)
                     /gene="plsX"
                     /locus_tag="JNY02_000058"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBB9431750.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(52772..52945)
                     /gene="rpmF"
                     /locus_tag="JNY02_000059"
     CDS             complement(52772..52945)
                     /gene="rpmF"
                     /locus_tag="JNY02_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBB9431751.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(52997..53518)
                     /gene="yceD"
                     /locus_tag="JNY02_000060"
     CDS             complement(52997..53518)
                     /gene="yceD"
                     /locus_tag="JNY02_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBB9431752.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            53717..54301
                     /gene="yceF"
                     /locus_tag="JNY02_000061"
     CDS             53717..54301
                     /gene="yceF"
                     /locus_tag="JNY02_000061"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBB9431753.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54413..55372)
                     /gene="rluC"
                     /locus_tag="JNY02_000062"
     CDS             complement(54413..55372)
                     /gene="rluC"
                     /locus_tag="JNY02_000062"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBB9431754.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            55945..59130
                     /gene="rne"
                     /locus_tag="JNY02_000063"
     CDS             55945..59130
                     /gene="rne"
                     /locus_tag="JNY02_000063"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBB9431755.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59327..60280)
                     /gene="flgL"
                     /locus_tag="JNY02_000064"
     CDS             complement(59327..60280)
                     /gene="flgL"
                     /locus_tag="JNY02_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBB9431756.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60292..61934)
                     /gene="flgK"
                     /locus_tag="JNY02_000065"
                     /pseudo
     CDS             complement(60292..61934)
                     /gene="flgK"
                     /locus_tag="JNY02_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62000..62941)
                     /gene="flgJ"
                     /locus_tag="JNY02_000066"
     CDS             complement(62000..62941)
                     /gene="flgJ"
                     /locus_tag="JNY02_000066"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBB9431757.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(62941..64038)
                     /gene="flgI"
                     /locus_tag="JNY02_000067"
     CDS             complement(62941..64038)
                     /gene="flgI"
                     /locus_tag="JNY02_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBB9431758.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64050..64748)
                     /gene="flgH"
                     /locus_tag="JNY02_000068"
     CDS             complement(64050..64748)
                     /gene="flgH"
                     /locus_tag="JNY02_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBB9431759.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(64801..65583)
                     /gene="flgG"
                     /locus_tag="JNY02_000069"
     CDS             complement(64801..65583)
                     /gene="flgG"
                     /locus_tag="JNY02_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBB9431760.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPAATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(65721..66472)
                     /gene="flgF"
                     /locus_tag="JNY02_000070"
                     /pseudo
     CDS             complement(65721..66472)
                     /gene="flgF"
                     /locus_tag="JNY02_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66492..67700)
                     /gene="flgE"
                     /locus_tag="JNY02_000071"
     CDS             complement(66492..67700)
                     /gene="flgE"
                     /locus_tag="JNY02_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBB9431761.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(67725..68420)
                     /gene="flgD"
                     /locus_tag="JNY02_000072"
     CDS             complement(67725..68420)
                     /gene="flgD"
                     /locus_tag="JNY02_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBB9431762.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(68432..68835)
                     /gene="flgC"
                     /locus_tag="JNY02_000073"
                     /pseudo
     CDS             complement(68432..68835)
                     /gene="flgC"
                     /locus_tag="JNY02_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(68839..69012)
                     /locus_tag="JNY02_000074"
     CDS             complement(68839..69012)
                     /locus_tag="JNY02_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HBB9431763.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69167..69826
                     /gene="flgA"
                     /locus_tag="JNY02_000075"
     CDS             69167..69826
                     /gene="flgA"
                     /locus_tag="JNY02_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBB9431764.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            69902..70195
                     /gene="flgM"
                     /locus_tag="JNY02_000076"
     CDS             69902..70195
                     /gene="flgM"
                     /locus_tag="JNY02_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBB9431765.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            70200..70616
                     /gene="flgN"
                     /locus_tag="JNY02_000077"
     CDS             70200..70616
                     /gene="flgN"
                     /locus_tag="JNY02_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBB9431766.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINCSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(70656..72191)
                     /gene="murJ"
                     /locus_tag="JNY02_000078"
     CDS             complement(70656..72191)
                     /gene="murJ"
                     /locus_tag="JNY02_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBB9431767.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72358..73224)
                     /locus_tag="JNY02_000079"
                     /pseudo
     CDS             complement(72358..73224)
                     /locus_tag="JNY02_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(73226..73873)
                     /locus_tag="JNY02_000080"
     CDS             complement(73226..73873)
                     /locus_tag="JNY02_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBB9431768.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYLLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(73884..74468)
                     /gene="rimJ"
                     /locus_tag="JNY02_000081"
     CDS             complement(73884..74468)
                     /gene="rimJ"
                     /locus_tag="JNY02_000081"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBB9431769.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HTCYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            74704..75912
                     /gene="mdtH"
                     /locus_tag="JNY02_000082"
     CDS             74704..75912
                     /gene="mdtH"
                     /locus_tag="JNY02_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBB9431770.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            75976..76623
                     /gene="grxB"
                     /locus_tag="JNY02_000083"
     CDS             75976..76623
                     /gene="grxB"
                     /locus_tag="JNY02_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBB9431771.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            76757..77317
                     /locus_tag="JNY02_000084"
     CDS             76757..77317
                     /locus_tag="JNY02_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB9431772.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77423..78469
                     /gene="pyrC"
                     /locus_tag="JNY02_000085"
     CDS             77423..78469
                     /gene="pyrC"
                     /locus_tag="JNY02_000085"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBB9431773.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHTRHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78543..78788
                     /gene="dinI"
                     /locus_tag="JNY02_000086"
     CDS             78543..78788
                     /gene="dinI"
                     /locus_tag="JNY02_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBB9431774.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEIPQETWESADDWFVSE"
     gene            79078..79332
                     /gene="bssS"
                     /locus_tag="JNY02_000087"
     CDS             79078..79332
                     /gene="bssS"
                     /locus_tag="JNY02_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBB9431775.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79447..80565
                     /gene="solA"
                     /locus_tag="JNY02_000088"
     CDS             79447..80565
                     /gene="solA"
                     /locus_tag="JNY02_000088"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBB9431776.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80613..80726
                     /locus_tag="JNY02_000089"
     CDS             80613..80726
                     /locus_tag="JNY02_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBB9431777.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            80987..81553
                     /locus_tag="JNY02_000090"
     CDS             80987..81553
                     /locus_tag="JNY02_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBB9431778.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81557..82132
                     /locus_tag="JNY02_000091"
     CDS             81557..82132
                     /locus_tag="JNY02_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBB9431779.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82174..83226)
                     /locus_tag="JNY02_000092"
     CDS             complement(82174..83226)
                     /locus_tag="JNY02_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBB9431780.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVSASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83451..84371
                     /gene="lpxL"
                     /locus_tag="JNY02_000093"
     CDS             83451..84371
                     /gene="lpxL"
                     /locus_tag="JNY02_000093"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBB9431781.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84543..84740
                     /locus_tag="JNY02_000094"
     CDS             84543..84740
                     /locus_tag="JNY02_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBB9431782.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            84764..>85895
                     /locus_tag="JNY02_000095"
     CDS             84764..>85895
                     /locus_tag="JNY02_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB9431783.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYWDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20618 a        22854 c        21157 g        21266 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgagtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttc gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgtgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgttgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatgg tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgcgtggtgt ggttctgata ttcctactct gctaatttgc tcagccccat
     6601 gtctgcgtaa gtgcgattta ctgcatttct caggtctgcg tagttctccg ttggcggctc
     6661 cggtgtgccc ttgctgctct gttttaccag cgggtcgccc accagccgcc cgtaagctgc
     6721 aatttgtgct gtcatattgc cacctcagtg aagcgatccg gcattgttct ttttcatctc
     6781 ttccatcatt ttttcagtga gcattacgca ctgaaacccc atgtttttga tgtctacggg
     6841 tttcatgcct ttgtcagtgg atatcacagg cagaatatcc tgatacacat cgcggtcagg
     6901 gtaactaagc acacaaagcg cccatgatgc cacgggtgag gatccttcgt ctgtttcaac
     6961 ccaccagcct ggtgtagctg cgcttatttg cagaatcgtg atccgtccgt tgtgcttgta
     7021 aaatttttca ccaaattttg gttcgctcat gattccacct ctccggtttt aacgttgatt
     7081 gttgttacct gttccgcttc ggcaatctcc cgctctgtca gcgtggcaaa atttgccgcc
     7141 gtcgtggtca tgaatgcgct tatcagttcg ggatgtgctt tcgcgtatcc ttcccccgcg
     7201 tggcggtcta tcgttctgat tgccaccttt aaagcgtgct ctgtcatgtc taacgcgcga
     7261 tatttcggtt ctgttctgtc tctgcgtttt ttgagtgatt tattaaattt ccctgaagtg
     7321 tgcatattta tttttacccc ctcgtttaaa aagttttgag ttgtgcctcc ccttgtctac
     7381 cttagtggcc ctcatgccag taatggcgct gctttcagcg gggtagagtg cttttatcca
     7441 ctatctaccc cgtgtctacc tccctgtctg attcaggtaa aatcaggtag agagggtaga
     7501 tagtgggtag acagtaaaaa aaggctacct acctaactta atgcactgaa ttaaatgtat
     7561 ttttctttac tcaggtagac agggtagaca gcaattacaa aaaattataa aaacgcgtcg
     7621 cactcgtctg ttgttattgc gttagtctgc gttactccct taactttccg cgtaatatat
     7681 tcatgtccgt aaactttcgc ggctggcttc atagccttgc caaagtcatt tacgtttagc
     7741 ggtttgctcc tgcctgcgta cgccataaac gccagataga cgcggtaaag gctgtttctg
     7801 gtcgtgtact tcactgaatc gccaccgccg cccatcatca ggccgcgtgc ttcctccaga
     7861 aaattcagga actggcaaaa ctcaataacc ggatccgtct gttgctttat tgccagagct
     7921 tcatcaccgt cacgctgttc cagtagtaaa gcccgtgctt tctcagagtc ggtaaagttc
     7981 gccagcaatc ggcggataat gacagggatt tcagccgcaa tcttttccgg tagctccctg
     8041 tctttttcgg cctcactaac gatattgtcg aaacggaaaa tcacgcgacg acgtgccaca
     8101 cctccggccc gttcggtgaa tatcatcggg ttattgttgg tcgccagcac caccgccctg
     8161 attacagccg tgaaacgctt ttcgtatttc gggttaattt ccacggggtc accgcccgtg
     8221 attttcttga tgcccgttcc ttcgcctgta tatttcggct ggtcagccag gacgataaga
     8281 cgactcccga caacctgcgg acgtccacca gcatcatcaa gcgatgtcat ttcagcgctt
     8341 accgtgttct gtttccctgc cagaaggctg gctatgtgtg tgaatgtact tttaccgctc
     8401 ccgccgtctc cggtggcctc aataaacatc tgccagtcgt accggttcgc cataatcatg
     8461 tacagcgcgg cacatatacg catcatcttg cgctggtctt ttccggctgc gtgctcaagc
     8521 catttatgaa agtttagcgc gttatcgcgg atgttctccc ctggtgctgg tggcgtgtac
     8581 tcaatgccgt tgtgcgtggt gatccagttc tccggcgtgt gcggggaaaa ttcccccgtt
     8641 ttcaggtcaa gcgcaccatt ggcgaacggc agcaaatcgc cggacggctc gcccattggt
     8701 tcggcaataa cttttaacgc ttccacggcg ttattgatta cgcgcttgct gaaagtggcc
     8761 ctgtgctctg aatagatcgc caccatttcg cggctaagtt ccattgtgct gaccggacac
     8821 cataccccgc cgcgccatac gtgaacgatt tcactttcct gatgcacaca aacgccatca
     8881 aagcgctcgg caagcagctg cgcgcgctca ctgtctgcca tttgtgccag ttgcgtattt
     8941 ttctgctccg gcatggtaag ccctgcggca atattttcac gctcagcatt cagatagcgc
     9001 cgccagtttt cacacttctg gacgtgcatt ccttcggggt aaaaatttgc gtcctgaata
     9061 tctgccgccg ccagcttctg accaatcttt ttggtctcca ccaaatccag ttctccggcc
     9121 tggtacagcc ttacgcgttt ttttccatcc ggaacaattt tcagtgcatc aagttcggca
     9181 agctgatttg gcccaagcca cacaggcggc acattatcgc cggatgcggg gccatcctgc
     9241 tcctgccatt gtttcgcgtg cgaccaggca tcactacccg caaagataat tacctctgtg
     9301 tctttgtgtt ttattccgcg tggctgtttt tttacgttcg gtgccagttt cattttttac
     9361 ccctgaatac gttaagcatc ttttttattt cctgaatatt ggcgcgtgct ttctccctgc
     9421 tggttggttt actgcggggc gctgcctgta ccagcgtgaa atcacgccgg aattgataaa
     9481 caggcatcac gcaatcatat tcgtaacctt cacggcggta ggtgatgcgc cgttctgcca
     9541 cgcctttaat cgttaccatg ccgccgtact ggtcgcggta aatatcaccg cgcgtaaatt
     9601 tagggtgagt gttgccactg gcagttaagc cagaatattt aagtttcatt atttttattc
     9661 tccggtgtgc tgttctttat atctgtcgtg caatagatct atttcttgca gttccattat
     9721 tacaggctca agaagcgtta ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc
     9781 attgtcgcca agtgtttcaa gccatatgcg caatatttcc agcatgtttt cactgtgaga
     9841 aagtgcaaga aatgcgcggt ctattgtttc gtggtaaata tcacgcatat taatccccgt
     9901 ccgtcgtttt tcttaaaata acctctgtca cgaaatcagc ataatcggcg gcgatattca
     9961 gtacatcaag cccgttgatt tatattctct cgtggaaagt aagaaaaaag ccgctctaat
    10021 aagttctggc attgacgaaa gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg
    10081 tttcagggaa ttaaaacgat catcacgcat aacccccccc attttcacaa tcggcaacaa
    10141 gaatattttt agcgtcattc agtgaccgcg ttgcgatgta tcggatacat tccagggcga
    10201 actgtgtccg gtctttctgt tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa
    10261 taagcgcgtg catcagcgta gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt
    10321 gcataggttt ccccctgacg aatacgggcg gcgaatacag caacacaacc ggacgggcaa
    10381 cggctacgcg cttcgcgttc cgtccaagcg gttacgtgga tgatttggga ttctccggta
    10441 ctcagtgcca gaaaacgcca cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga
    10501 tttacggcaa ccataacggc tcctagttta cgttgttggt tagaagcccc gttactgctc
    10561 ctacagtgcg gggctttgct ttttgtgttg attgtgtgta atgtgtcatt acacataaat
    10621 acattacatg aggtgtaatt aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc
    10681 cgttccagtt ccgtttagat cctgacttac gggaaaagat ggaaaaggca caacaacagg
    10741 acggcgatga ttctcttgct gcctggataa agcgaataat ccgcaaagaa ttacagcaac
    10801 gcggcataga acccaaagga taaaaactat cagcgccgtg gtgtgaggta ctacggcgca
    10861 ttgctttaca ggcgcacaca atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac
    10921 gaaaaagacg acgcagaaag aaagagcatg aatcagaacg attcgcgccg tgttcttttg
    10981 cccttgagaa gtaccttaaa gaatactcat caaaggagag aacttcgcag ttatggcaac
    11041 gcactgaacc agactgattg cattgcccac cagcctgata gcggctatca tccccgtgct
    11101 tatgtttggg atcacacaca caaacggcgc agcgggttat ctgttcagaa aggcggctcc
    11161 atttcggggc cgcttttttt atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac
    11221 cagggcgaac gaatccccgc ccacgttctg gcgtatattc aatcttcatg gttatagctc
    11281 tgtgttcaga tgattgatgt gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct
    11341 tgcagctatc cttccaggcc agaacctcgg ataaagacca gccaacggaa cgaccgccaa
    11401 gtttacgacg tgatgggaat tgtccggcct tttccaggcg gtagcggcat gagcggctaa
    11461 ggcctgttag cttttcgcat tctttttcac gtataaaccg atcagtgctt aacactattg
    11521 ccccctttcg tttcttaaag agttatttcg tgttctattg cgttgggatg tgtctgattg
    11581 tgtcaggatg attcagagtt ggcaaatgtt gaggccgtat ggtttacaga aagaggaaca
    11641 atcaggataa aatcatttaa attcatgtta atacaaaggc ataaaatatt gtttcatgcc
    11701 ttttttctcg ctatttaaag agtgattcgc tagtgtataa aaaaccagta acacgttaaa
    11761 aatcagctac ttataaatcc gtacactttt tcacctcttg ttcgtagttg ttccgcattg
    11821 ttgctcattg ttgcacgttg tatctgttcg catatccagt atgcgcatac tgaaaaaaca
    11881 cgaaaaaaat tattttcttc tggctactgg tagcgtggtt acgttttcat gtgttcccgc
    11941 cagtatccct aaccgctccg tccacatatc cagcgcattg cgtttagcat ccagataacg
    12001 ggaatgatta taaactcgct gcattcctgg catctggtgg cctgtaagct gctccacgac
    12061 gtgaggatcc acgcctaaat cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag
    12121 tgaccagtga gggtgattta gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt
    12181 ttcctgtttc acttccccca gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt
    12241 gacgaacggc agtattgctt ccggtatggg ccggaatatt gccaccttcg ttttgctgtg
    12301 ttctttcggc acggtccaga gcatttcggt aaaatcccac tcgctgatct ccgataacct
    12361 cagttctacc gtccggcatc cgaacacaat caggaggcgg attaacgcga tgtagtaggg
    12421 ggagaatatt tttttgtcca gtgcctgcaa taattcgccc agttctttgg tgcttaagac
    12481 acgctcgctt atatccggtt ttttcccaac gtccgccaca ctcatatcat caagaacgtt
    12541 gctgattgca tagcgccgcc tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa
    12601 gaatccggca gtaacaggcg ttcgctttgc cacctggtca aaacaggcca gccagtgccg
    12661 tagctcgcat ttatccagcg gcatagcacc aatgtgctgt attacgtgat tattaaggcg
    12721 ctttttcagg gcggcataat ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt
    12781 gagcgcatcg ccaaccgtta ccggctttaa cgcttcctgt acggtgtaat taagctcatg
    12841 acgtggattt ttcccctctg ccagccatgc gcgacactgg gcggcttttt ccctggctga
    12901 tttcaggctc agatcaggat aatttcccag cttaatgcgt tccggtggtg ccccccttcc
    12961 cgttccggcc ctgtaagtga aataccaggt taaaaggcca ctggttgaat gcctgacgct
    13021 caggtttcca ccgtcattaa gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct
    13081 cagctgtgta tcgctcagtt tgttaagtgc tctgctcata attttgattc cggtcatcac
    13141 aattaactac accattaact acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc
    13201 tggaacaagg aaaatcacaa atctgtttat aatcaaaaac ataatgaaca atctgaaaca
    13261 ttatgaaacg gcaaaaaaca ctaattgata aaatatgtcc atacttcacg cattacgtta
    13321 agcatccgtt ataatcggtt gcagatacca gcctgtggat gcttaacatg gaataccaac
    13381 tcactcttaa ctggcccgat tttcttgaac gtcactggca gaaacgcccg gtggtgttaa
    13441 aacgcggctt taataatttt attgacccga tctctccaga cgagttggca ggtctggcga
    13501 tggaaagcga agttgacagt cgactggtca gtcaccagga tggcaaatgg caggtcagcc
    13561 acggcccgtt cgaaagctac gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg
    13621 cagtaaacca ctggcatgag ccgaccgccg cgctgatgcg accgttccgt gaactaccgg
    13681 actggcgtat tgatgatctg atgatttctt tttctgtacc tggcggcggc gtcggcccgc
    13741 atctcgatca gtacgacgtg tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg
    13801 gcgaaaagct gcaaatgaaa cagcactgcc cacatccaga tctgttacag gtcgatccgt
    13861 tcgaagccat catcgatgaa gagctggagc ctggtgatat tctttatatt ccgccaggat
    13921 tcccgcatga aggctacgcg ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc
    13981 caaatacgcg ggaattaatt agcggatttg ccgattatgt gctgcaacgt gaactgggcg
    14041 gcaactacta cagcgatccg gatgttccac ctcgcgctca tcctgcggat gttctgccgc
    14101 aagagatgga taaactgcgt gagatgatgc tcgaattgat caaccagccg gaacacttta
    14161 agcaatggtt tggcgagttt atatcccagt cacgtcatga actggatatc gcgccgccgg
    14221 aaccgcctta tcagccagat gaaatctacg atgcactgaa acaaggtgat gtgctggtgc
    14281 gcctgggtgg tctgcgcgta ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga
    14341 tcgattcccc gcaccgtccg gcactggatg cactcgccag caacattgcg ctgactgcgg
    14401 agaattttgg cgatgcgctg gaagatccgt cattcctcgc gatgctcgcg gcgctggtca
    14461 atagcgggta ttggttcttc gaagggtaag tttggattta agccggatgc agcatcacat
    14521 gccgcatccc ttttcgctta cttccgttgc gccgttaact cggcaatacg gacgatcacc
    14581 tgcaccgctt tttccatacc ttccagaatc acaaactcat gcttaccatg atagttgtaa
    14641 ccgccagtga acaggttcgg gcaaggtaat cccataaacg acaactgcgc gccgttggta
    14701 ccgccgcgga tcggtttcag ttccggttca atatcgcagt cacgcatcgc ctgctgggcg
    14761 atatcgagaa tatgcggatg ctcaaccact ttctcgcgca tattgtagta actgtcttca
    14821 atcaccaatt caatgtagca atcaggatgt aaccctttgc ccactttttt ggcgatctcc
    14881 atcattttac gtttacgcgc ttcaaactgt ttacggtcga aatcacggat gatgtagtgc
    14941 atatcggccc gttcaacggt gcctttcatg ctcgccaggt gatagaaacc ttcatagcct
    15001 tctgtcattt ccgggctttc atccgccgga acttccgcat gaatacgtgc cgccagcgac
    15061 agcgcattta ccatcactcc tttcgccgtg cccggatgaa cattgttacc gacaattttg
    15121 atattgaccg atgcggcgtt gaagttttca aactccagtt cgcctacgcc accaccgtca
    15181 acagtgtaag cccagcgggc atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc
    15241 acttcttcat ccggggtaaa ggcgacgcga atatcaccat gcggaatgtt tttctgttgc
    15301 aataccgcca gcgcggtcat gatttctgca atacctgctt tgtcatcggc acctaacaag
    15361 gttttaccat cggtggtaat caacgtctga cccagtagct gatgcagcac cgggaacata
    15421 accggtgata aaacttcatc gccgataccc agcgcaatat cgccaccgcg atagttttca
    15481 acaatttgcg gattaacatt tttgccgctg caatccggtg aggtatccac atgagaaata
    15541 aagccaatcg ccgggatatc gccagggacg ttagccggta acgtcgccat caaagtgccc
    15601 ttctcactta aggtcacatt gataagcccc atttcttcga gctgctcttt cagcagatgc
    15661 aataacttcc attggccttc cgtgctggga acctgtctca cccctgcttt tgattgggta
    15721 tccagagaca cgtagttcaa aaatcgctca agtagtttat ccatgtagtc accctcactt
    15781 tttgtgacaa cattattaag aagcaagaaa agacaaatat tgcgtcaggt cacttttacc
    15841 cctgcaagcg ggaatattta tcagcattac cttaatgaat ataaagctaa gccagtaatt
    15901 cacaacaaag attggcgatt caagcggttt gccgtagaat taccgccctc attaagagtc
    15961 accaaggtgg ttaaccacaa accccgcatc ggtaagccat ccgttgcgtt tacatgggac
    16021 agagtaaaaa attgaataaa caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa
    16081 ttcgcaaatg ctttgatggt aaagaggtca ttccccagct ggatctgact atcaacaatg
    16141 gcgagttcct cacgctgctt ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga
    16201 ttgcaggtct ggaaactgtt gattccggac gcatcatgct ggataacgag gacatcaccc
    16261 acgttccggc ggaaaaccgc tatgtgaaca ctgttttcca aagctatgca cttttccccc
    16321 acatgaccgt gttcgaaaat gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg
    16381 aaattacgcc ccgcgtgatg gaagccctgc ggatggtgca gctggaaacc ttcgctcaac
    16441 gtaaaccgca tcagctctct ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg
    16501 ttaacaagcc tcgtctgttg ttgctggatg agtcgctctc agcgctggat tacaaactgc
    16561 gtaagcaaat gcagaacgag ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt
    16621 tcgtgactca cgaccaggaa gaagcactca ccatgtcgga caggattgtg gtgatgcgcg
    16681 atggtcgcat tgagcaagac ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt
    16741 ttgttgccgg gttcattggc gaaattaata tgtttaacgc cacggtgatc gagcgactcg
    16801 acgagcagcg cgtacgcgcc aacgttgaag gccgcgaatg taatatctac gttaacttcg
    16861 ccgttgaacc ggggcaaaaa ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag
    16921 agattaacga cgacaaccac gctgaagggc tgattggtta cgttcgtgag cgtaactaca
    16981 aaggcatgac gctggagtcg gttgttgaac tggaaaatgg caagatggtg atggtcagcg
    17041 aattcttcaa tgaagacgat cctgactttg accactctct cgaccaaaaa atggccatta
    17101 attgggtaga aagctgggag gtcgtactgg ctgatgaaga acacaagtaa gttccagaat
    17161 gtagtgattg tcactattgt cggttggctt gtgttgtttg tctttctgcc caacctgatg
    17221 atcattggca ccagcttttt gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg
    17281 ctggataact acacgcgtct gctcgatccg ctctattttg aagtgctatt gcactcgctg
    17341 aatatggcgc tgatcgccac cctcgcctgc ctggtgctgg gctatccatt tgcctggttt
    17401 ctggcgaagc tgccacacaa ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc
    17461 tggaccaact cattgattcg tatctacggg ctgaaaattt tcctcagcac caaaggctat
    17521 ctcaacgagt ttttgctctg gctgggcgtt atcgacacac caatccgtat catgttcacg
    17581 cccagtgcgg tgattatcgg tctggtttac attctgctgc cgtttatggt gatgccgctg
    17641 tactccagta tcgaaaaact ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc
    17701 agcaagttac agacttttat ccgcatcatt attccactga cgatgccggg gattattgcc
    17761 ggatgtctgc tggtgatgct gccagcgatg ggcctgttct atgtgtccga cctgatgggc
    17821 ggtgcgaaaa acctgctgat cggtaacgtc atcaaggtac agttcctcaa tattcgtgac
    17881 tggccgtttg gtgcagccac cagcattacg ctgactatcg taatgggcct gatgttgctg
    17941 gtttactggc gcgcttctcg tttgctgaat aagaaggtgg aactcgaatg atcggtcgac
    18001 tgcttcgcgg cggttttatg accgctatct acgcgtacct gtatatccca atcattattt
    18061 tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa ctggcagggt tttaccacca
    18121 aatggtatag cctgctgatg aacaacgaca gcctgttaca ggcagcgcag cattcactaa
    18181 caatggcggt gttttcggcg acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc
    18241 tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga
    18301 tgtcaccaga tatcgtgatg gcaatttcgc tgctggtgct gtttatgctg ctgggtattc
    18361 agcttggctt ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg
    18421 tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg gcgaaagatc
    18481 tcggtgccag cgaatttacc attctgcgaa aaatcattct gccactggca atgccagcgg
    18541 tggcggcggg ctgggtgtta agctttaccc tgtcgatgga cgatgtggta gtttcttcat
    18601 tcgtcaccgg accaagttat gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg
    18661 gcgtatcgcc ggaagttaac gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg
    18721 ttattgccag ccagcttatt gctcgtgata aaacgaaagg taacacaggg gacgttaaat
    18781 gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg gcactgggca tgagcgccgc
    18841 tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg tgccgccagg
    18901 actgcttgaa cagttcacca aagaaaccgg tattaaggtt atctattcga cttacgagtc
    18961 gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcctatg acctggtggt
    19021 tccttcaacc tattacgtcg ataaaatgcg taaagagggg atgatccaga agatcgacaa
    19081 gtcgaagtta acgaacttca gcaatctcga tccagacatg ctcaacaagc cttttgaccc
    19141 gaataacgac tactccattc cgtatatctg gggtgcgacg gcgattggtg ttaacggtga
    19201 tgcagtggat ccaaaatctg tcaccagctg ggccgatctg tggaagccag agtacaaagg
    19261 cagccagctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc gtaagctggg
    19321 ctactccggt aacaccaccg atccgaaaga gattgaagct gcatataacg agctgaaaaa
    19381 actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt acatggaagg
    19441 cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc aggcgggtac
    19501 gcctattgac gtggtgtggc cgaaagaagg cggcatattc tggatggaca gcctggcgat
    19561 cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc
    19621 ggatgtggca aaacaggttg ctgaaactat cggttatcca acgccaaacc ttgcggcgcg
    19681 taagctgtta agtccagaag tggcaaacga taagacgctc tacccggatg ctgaaaccat
    19741 taaaaatggc gaatggcaga atgacgttgg cgcagccagc agcatttatg aagagtatta
    19801 tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggtattac cgcccgtttt
    19861 ttagttagca aggatgtggc taatgagtca ggacagtaaa gttttatccc gctctttcgt
    19921 cggtattgtg gtccgcttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct
    19981 ttataatccc tccagcaact tttcaacaaa ttctggcacc acctggcttg ccgggccgta
    20041 atatttctcg gcaaattcat taccaacctg actcggttca agattcagtt caacggtgtg
    20101 agcaccatgt agtttggctt cgtgtacaaa cccagcagcc ggatagacat gcccggaagt
    20161 accaatggca ataaagacat ccgccatcga caacgccata taaatttcat ccatgccgag
    20221 tggcatttcg ccaaaccaaa cgacgtgcgg acgcaagggt gccgggaact ggcaacaatg
    20281 gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac tttgcgaaca
    20341 acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg cgcgttcatg
    20401 caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt
    20461 agccagcgca agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg
    20521 ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac
    20581 atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga
    20641 ttccgcagaa attcctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg
    20701 caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt
    20761 tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta
    20821 atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg
    20881 ttcaatgcgc ggaacacgag ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg
    20941 cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac
    21001 aatggtcagg atatttccca gagaaaccgc taataaatcc agataacgct caacgtgcgc
    21061 ccttgcctgc tcatcgcctt gatcataaag cgcaataatt tcgggagcct gcaacggttg
    21121 atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca
    21181 gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca tggttaacgc
    21241 atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac cagtaatcgg
    21301 tttgccgttg aaaaccagcc cgccgccaac gccggtgccg agaattaacc ccatcaccag
    21361 tggatattgc gtaaattcat cctcccaggc ttctgaaagg gcaaaacagt tggcatcgtt
    21421 atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct
    21481 ggcagcgggg acattagcgg catacagctt accatcttct gtttccggca ttcccggaat
    21541 accgatgccg acagaacctt tacagccaaa acgttggtca gcttcagcta ccagctcaca
    21601 cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg
    21661 caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc
    21721 gtaatacatt gcctctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg
    21781 cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca
    21841 atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa
    21901 taaaatagat atcgctggag aggaactgat gaccgatcag cttttcaatc cactcaataa
    21961 tcggggtaag ttgcagtgaa acaactacgc cgataaccac cccacacagg ctgccgaaca
    22021 gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca
    22081 gcgttcttaa tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga
    22141 cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga
    22201 tcatctggat atcgcgatac atatagccgt aagtaccaat ccaactttta atataaacat
    22261 agctgttggt cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca
    22321 ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca
    22381 gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt
    22441 gcaaacgcac acgttttggc tgcatcagtt tatgctcggg attcgagttg gggatcatga
    22501 tcgacaccca atcgccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa
    22561 tttgctgttc gcccgcttta aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg
    22621 cgctcagacg ctgttcctgt tgtgggttaa cgcccttcac ctggattgcg cgcagattcg
    22681 ctccactttc caccagaccg gtgaaattga tatacggcgc agcggcggca atacccggca
    22741 ctttctgcac gttatccagt gcttcctgcc agttagtcca tggctgattt accgcttcga
    22801 tttcgccatg cggcaccacc gccagaatgc ggttattcag ttcgcgttca aagccgttca
    22861 tcgcgcttaa gccgacgatc aataccgcca cgccgagggc aatgccaatg gtagaaatga
    22921 cagagattag cgacaccatg ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca
    22981 ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg
    23041 tccatcacgc atttccagtt ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac
    23101 caccaggaaa gcggtgccct gcaagcgatt caactctcca agcaactgga agatgctgtc
    23161 ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc agtaccagac gcggattatt
    23221 gaccagcgca cgggcaatcg ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg
    23281 gtgattcgca cgatgctcca gccccaccgc ttttaacatc tccagtgcac ggctgttgat
    23341 ttcagcgggc tttttcttgc caatcagcag tggcatagtc acgttttcca gggcagtaaa
    23401 atccggcagc aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagttctgc
    23461 tttcgccgct gaagacagtt tgctcatcgg ctgaccgtta aagatcacat cgccggaggt
    23521 tggcgtatcc agcccgccta acaggtgcag caaggtactt ttaccggaac cagagctacc
    23581 gacaattgcc atcatttcac cttcgcccac gctgaaactg acattgtgca gcacatcggt
    23641 ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt
    23701 cataacgtaa agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag
    23761 acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag
    23821 ccaccggcag cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact
    23881 ggctggcaag cagtgcgcca agcgccgctc cgaggattgc accgataatc ccggcgctgg
    23941 ccccttgcac cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga
    24001 tcgcgacttc gccctgcttc tccatcacca tcagccccag cgaggtaata atgttaaacg
    24061 ccgcaacagc gacaatcagg ctcagcagta agcccatcat atttttttcc atgcgtacgg
    24121 cctggaacag ctcgccttta cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt
    24181 tttgctgact taaagagtca actttcagcg gctcatccag ccacaaacgc cagccggtaa
    24241 tattggctgc cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat
    24301 agccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg cgctgacttg
    24361 gaatacgccc catcggcgtg aactggctgg cagatggcac catcacgcgg atttgatcac
    24421 cgcgattgac gcccaactgt gaggcaagtt gttcgccgag gatgacatta tatttccccg
    24481 gctcgaggtc agtttgtttc acattgacca gatacggcgt tagtggatct ttttgcgccg
    24541 ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac
    24601 cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt
    24661 gctgcgggtt aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga
    24721 tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca
    24781 tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatccg
    24841 ctgcacgccc acgcatgtaa cgcaagccaa taaatagagc gacaggttgg tacatgaaat
    24901 ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc
    24961 tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat
    25021 tgccatgttt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag
    25081 acagataccc attacacctc gggtcataac cagcaggatc aaccgcatgt aaattaaaaa
    25141 agagcgtgtt gttctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg
    25201 tatttgtctg gtggtgattt atcactcggt cattaccttt tatccgcata tgaccacttt
    25261 tcagcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc
    25321 cccctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc gctatatcga
    25381 cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct gggtgctggc
    25441 gctttggggc gtggtgcagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    25501 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    25561 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    25621 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    25681 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    25741 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    25801 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    25861 gctggtatcg actgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    25921 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    25981 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    26041 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    26101 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    26161 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    26221 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    26281 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    26341 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    26401 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    26461 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    26521 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    26581 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    26641 gcgtactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    26701 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    26761 aactggacag cgccggttat cgctatgttg accaggtgat ggagcacggc gaatacgcca
    26821 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    26881 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    26941 aggaagtaga agcgatcaat ctgctgcccg cacacgaatt tccgaccgat aaagcggcaa
    27001 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    27061 tttaccagca agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccattgt
    27121 tcttcagcga accactgccg ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    27181 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    27241 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    27301 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    27361 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    27421 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    27481 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    27541 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    27601 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggtgcgtaat ctggcgctga
    27661 tctgcgaaag cgatctgctc agtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    27721 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    27781 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    27841 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    27901 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    27961 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    28021 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    28081 aacacgatcg tgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg
    28141 atcaggcgca ggccattaat gcggtactta acgacatgtg tcagccgctg gcaatggatc
    28201 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    28261 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    28321 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    28381 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    28441 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    28501 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    28561 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    28621 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    28681 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    28741 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    28801 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    28861 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    28921 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    28981 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    29041 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    29101 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    29161 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    29221 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    29281 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    29341 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    29401 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    29461 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    29521 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    29581 gcgggatgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    29641 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    29701 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    29761 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    29821 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    29881 tgtttattac aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt
    29941 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    30001 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    30061 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    30121 ctcgctctgt tacaggctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    30181 ttaacgatcc cgttgccaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    30241 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    30301 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    30361 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    30421 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    30481 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    30541 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    30601 ctctttagct aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    30661 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    30721 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    30781 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    30841 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    30901 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    30961 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    31021 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    31081 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    31141 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    31201 tgagggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagaaa
    31261 ttacttaacg atcgataact aaatgataga tgtgatatgg atcacataca agatattttt
    31321 tataacaatc attaattaat ttaaaaatct ttaaatttca gtcaaataga aagcaattat
    31381 ttttatggga tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac
    31441 agtcaccagg tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg
    31501 tggccgccca aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg
    31561 gcaacacggt tatgaagcga cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc
    31621 gcccacgcta tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg
    31681 ctatatcgat cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt
    31741 ggagtctgcg ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac
    31801 cccggcaggc tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat
    31861 cgccaatacg ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg
    31921 tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt
    31981 ccttaattgt attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa
    32041 actgatgcag attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc
    32101 aaaaaaagcc cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat
    32161 actttgttat tcaaaatcag ttgcccgtta ctatctagcg ggatctgcgt acccggatcg
    32221 cggtccatgc ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc
    32281 attttttctg acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg
    32341 ctttcctgga gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca
    32401 acagtggcga catctttacc gcgaccacca ccaaactgat gccctatcac gccaccagca
    32461 acagcgccga gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc
    32521 actgtgacgt tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca
    32581 gaaacaacct gagcgtattg cgggccccgt tcaaacacgt tcagactggc cactgccgct
    32641 acgcccaacg cagctgcgac accaatcccg atacccgcca acattgattt attcacggga
    32701 acacctcctt ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga
    32761 cagaagatga aaagcgcggg aatgactgga aatacttaag gattcggagg agtctgaagg
    32821 aatgcctgat gcgacgctta acgcgtctta tcaggcctac cgctcgtacc atttgtccag
    32881 ggcggataag gcgtttatgc cacatcagcc agtgtacgtc gattaatgca acttcaaacg
    32941 cggacggata acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc
    33001 atgcagcgca atctgatgca ttcggtatag cgagatatat acaaagcgcg caattcgtcc
    33061 ttcaatcatc attgagccgc gcgtcaggtt acccatcagg ctaccaacgg tggagaagtt
    33121 cgacagcgat accagcgaac catgatcttt atactgataa tttttcagcg gcttaccgtt
    33181 catctgcgcc agaatgttgt tcattgcgca agtcgccatc tggtgtgcag cctgagcacg
    33241 cggcggaaca aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat
    33301 gtctggatcg cgagtggttt gcagcgtcgg ttccaccacc agctggttga tacggttagt
    33361 ttcaagacca ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccataccat
    33421 cagatcagcc tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt
    33481 gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc
    33541 agagatacgt ggcggtaatg caggcaaaat acgttctccc gcttctacca gcgttacgtt
    33601 cagggcttcg ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga tcgcgttgtg
    33661 caattcagcg gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt
    33721 gccatttgcg cccaggttgg cagagtattt caggaacaga ttcagcatct cctggtggaa
    33781 gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc ctggcgtatt
    33841 gaaatcgtta gaggtgctac ccagcgccat gaccagggtg tcataggcga ttttacgttc
    33901 cggaaccaac agttcacctt tctcgtcgcg tagttctgca atagtgattg ttttcgcttc
    33961 acgatcaata tcaatgacgg aacccaactg gaactggaaa ccatgattgc gcgcatgggc
    34021 cagatagctc aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag
    34081 tggtttccac aggtggctgt ggttacgatc gaccagcgta attttggctt ttttcttacg
    34141 tcccagctta tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat
    34201 cacaatcttt ttcaatggcg tagtcaacgt gaccccctta aatttattaa ccaattgtta
    34261 attaatggtt attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt
    34321 cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt
    34381 ttgttgctta aaagataggc aactttaccg ggggagatca aaaaatagcg tttattattt
    34441 gagcaaattc ggaagagtaa tatagggtga agcgtatatc gagggcgttt ggtcgtcctg
    34501 ataagatgcg cagcatcgca tcaggcattg atgctcgact gtcggatgcg gcgtggacgc
    34561 cttatccgac ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg
    34621 agggcattta cccgagggtt ttgaacgctt taatgcgctg taaatgcggg gagatattct
    34681 tgaatttgtg cgtctgctct tcgtcccaga caatctcgta ataatgatgc aactcttcag
    34741 atgtccgctg gctgttaagt gcttcatcat tacgcgacaa aataaccaga caacgatcgc
    34801 gattcttctc gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc
    34861 gatcaatctt gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc
    34921 ggatatcgca aagaaaacca atccgttccg cccagtatcc gcccaggcca acgccgcaaa
    34981 ttagcggacg ctcgtcaacg ttcagttgca acattttgtc cacttcttta agcagatgct
    35041 gcatatcatg tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa
    35101 actgcaattg taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat
    35161 agataatcat cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag
    35221 cgttcatgta actgattcag acgggtgctg atcgctttcc agcgagccga gtccatcagt
    35281 tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt
    35341 gacagattat ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg
    35401 caacccgcat ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggcacct
    35461 tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac ccagttcctg acgcaaaacg
    35521 gttttcagcc agtagggaga accgctcgcc ggacgcggat caacatcact gtagatcaca
    35581 tgcgcaggca taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata
    35641 tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg gtgtttcttt gtgtgagtct
    35701 gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc
    35761 ataccatcaa taaaccggct ggcaattgcc agggcttttt gtggatcggc atgataagaa
    35821 cgctcgccaa tcgccgcgct gatatgcccg acatccagca ctggcgcaaa gctgatatca
    35881 atatccatag cgatcatttc gctggccatc agccaacccg cctcttgcgc cagtttgccg
    35941 ccctcttcca ttcccaacag cgcagcaaag gattgtgctg ccggtaagcg ggtaaaacct
    36001 tcgcgaaaac gctgcacgcg tccaccttcc tgatctaccg ccaccaccag atgattgcgc
    36061 gatgctgcgc ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc
    36121 gtaaagagaa tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc
    36181 agttcgtaac cttcgacatc caacattact ggacccacac tgctctcctt attgttttat
    36241 taatagctgc cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg
    36301 ccagcggtac tcaaaccacc ctcctttgag catcagcagc cagggaaacc atcgcctgac
    36361 ctgacgccat aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg
    36421 ccggtgctgt tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc
    36481 tccagcatac tcccagtcga tgagttttaa ccctgatgcg ctatgcacta aatttccggc
    36541 gtggacatcc atatgcaatg gacttaagcg taaaggtcgt ggttcccgcg ctttgcgcag
    36601 acgttttaac attcgcagcc aacccacagt ccgccgcgcc ggatcgcttt gctgccagta
    36661 cagttccagt aagggcaaca gcgttattcg ccagccaaaa cgtggttgtt gatgtagata
    36721 atacagcaag cctgccagtt cgttggtatc cggcaaatac gtttttaccg cgccgggcag
    36781 atagtcgact accatccagt cacggagata taaatgcggc ttcggtgcaa tgcttgcggg
    36841 tagttgtgat aaagcccgat actggcgcaa gaacgcggac tgcggcgcat caggatcgtg
    36901 cggctgacgc acaacaaaac gctggccctg atgttcaatg agaaaactac cgccactaag
    36961 cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc
    37021 gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg
    37081 tctgcaccag catcagctgc atttgtaggg tcggagcgtt aacgttgcca gaggcgctgg
    37141 agtacagcac gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtgc
    37201 ctaaactgtc ctgcggcgac aaacctaact gttgcttagc catcgacagt tgctgggcgg
    37261 aaaccagggt aaatttcccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg
    37321 cggcattcag cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg
    37381 cagtcacccc gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt
    37441 cataatggcg aatatgcggc gcaggcggtg ccgttcgatc ttcgtgctca attgggcctg
    37501 gctgctgcgg gatcgtcggc accgagggca ctgtggggac aggctgttgt ggctcggctg
    37561 gttgttccgg cgctggtttc acttcttcta ccggtgcagg ttcacgttgc cccacacacc
    37621 cggcgagaaa catcgccagc gcggtaatca aggcgtagcg actcattttt gtcatcaaga
    37681 ttcacccctt tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
    37741 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
    37801 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc
    37861 ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac
    37921 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
    37981 atcattcacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca acaccagaga
    38041 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
    38101 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg
    38161 ttggtattca tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt
    38221 gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct cagctgacac gtcgttcaca
    38281 gtcggaatga ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
    38341 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
    38401 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
    38461 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
    38521 ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
    38581 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgac
    38641 aaatatgaat gcgtatattt ctcatttgca tttgcaagca aaattattcg cacactaaat
    38701 ataaccgctc atcaccctcc tccggcatga gcctgacgac aacataaacc aagagatttc
    38761 aaatgctttc aacacaattt aacagggata atcaatatca agccatcacc aaaccgtcac
    38821 tacttgccgg ttgcatagca ctggcactat taccttctgc cgcttttgct gcaccagcca
    38881 ctgaagaaac ggtgattgtt gagggttcag ccacagctcc agatgatggc gaaaatgatt
    38941 acagcgtaac gtctacctct gcgggtacca aaatgcagat gactcaacgt gatattcctc
    39001 agtcggtcac tattgttagc cagcagcgga tggaagatca gcagttacaa acgctgggcg
    39061 aagtgatgga aaacacgctg gggatcagca aaagtcaggc ggattccgat cgtgctcttt
    39121 attattcccg cggattccag atcgataact atatggttga tggtatcccc acctattttg
    39181 aatcgcgctg gaatctgggc gacgcacttt ctgatatggc actgtttgaa cgcgtagaag
    39241 tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg gcaattaata
    39301 tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga tgtctcggcg gaatacggta
    39361 gctggaacaa agaacggtat gtggcggatt tacaaagccc actcaccgaa gacggtaaaa
    39421 tccgcgcgcg aattgtcggc ggctaccaga ataacgactc atggctggac cgctacaata
    39481 gtgaaaagac cttcttctcg ggcattgtcg atgctgattt aggcgatctt acgacgcttt
    39541 cagccggtta cgaatatcag cgcattgatg ttaatagccc aacctagggc ggtttaccgc
    39601 gctggaatac tgatggcagc agcaacagtt acgatcgcgc acacagtacc gcacctgact
    39661 gggcgtacaa cgataaagag atcaacaagg tctttatgac cctgaagcag cggtttgctg
    39721 atacctggca agcgacactg aatgccaccc actctgaagt cgaatttgac agcaaaatga
    39781 tgtatgtcga tgcctatgta aacaaagcgg atggtatgct ggttgggcca tacagtaatt
    39841 atggacctgg ctttgatcat gtcggcggca ccggttggaa cagtggcaaa cgtaaagttg
    39901 atgcgctgga tttgttcgct gacggtagtt atgaattgtt tggtcgtcag cacaatctaa
    39961 tgtttggtgg cagttacagc aaacaaaaca atcgttactt cagttcatgg gccaacatct
    40021 tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa accgactggt
    40081 caccacagag cctggcgcag gacgatacca cacatatgaa atcgttatat gctgccactc
    40141 gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc acgttatacc aactggcggg
    40201 ttgatacgct gacttacagc atggagaaaa accacaccac gccttacgct ggtctggtgt
    40261 ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc cagccgcaaa
    40321 atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac tacgagctgg
    40381 gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc ttccgtattg
    40441 agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac ggcgaaaccg
    40501 cctataaagc ggtggatggg acagtcagta aaggggtgga atttgaactc aacggcgcaa
    40561 ttaccgacaa ctggcagctg acatttggcg caacgcgcta tcttgcagag gataacgaag
    40621 gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc agctatcggt
    40681 tgcctgtcat gccagagttg acagtcggcg gtggtgttaa ctggcaaaat cgcgtgtata
    40741 ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca aggtagctac gcgctggtgg
    40801 atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac gtcaataacc
    40861 tgttcgacaa aacctacgat accaacatgg aaggttctat cgtctacggc gcaccgcgta
    40921 atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag gcagccatct
    40981 ggctgcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac tcatccatct
    41041 cggttttcag gttatcggat ttagtaccga aaatcgcctg aacaccagaa ccagcaacca
    41101 ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc cactttagac acatcagcaa
    41161 cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatgttttct ttaccaccaa
    41221 atgcagcaac cagagccggt gccatttcgc tggtacctgt tgcttttgca tcttcagtcg
    41281 cgtcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg aagatggtgt
    41341 agtaaacaat cgcataaccg ataccgacga tcgggaacag ccacagtttg ctgctgttac
    41401 cagacagaac gatgaagtcg atcagaccgt gcgagaacga cgtaccgtca cgcatcccca
    41461 gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg tacaggatcg
    41521 gcgcaacgaa catgaagaag aactcgatcg gctcggtgat accggtcagg aacgaggtca
    41581 gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta gcagagtgcc
    41641 agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca ccagacagtt
    41701 tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa acctgacctg
    41761 ctgcgttggt gtattcacca atctgcatct ggaaaggtac gttccagatg tggtgcagac
    41821 caaacggtac cagacaacgt tcgatgaaac cgtaaatgcc aaacgcaact accgggttct
    41881 ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc caaatgaagg
    41941 acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca aagcgtttac
    42001 cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta aacatgtacg
    42061 ctgcaatcgc accggagata atccctccga gtacgccagt atccgccaag tgtttagagg
    42121 cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg gttttaacca
    42181 tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg gtaaagccga
    42241 gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg cctgcttctg
    42301 ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg acgcccagca
    42361 gaatacctgc gataggtagt acggataccg gcagcatcag cgatttaccg accttttgca
    42421 ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatagg tgcttttttt
    42481 acgttctcac gcgtggcaag gggggagagc ctcgccgtgt acagggcatc taagcgccct
    42541 ttatttatta cacagagtaa aataattcag tgccaatttg tttgacggct atcacgtttc
    42601 aacttcagat cattggagaa gttgcacatt ttgacaaaac atatgtcaca atatttcaaa
    42661 cgcggttatt caccgcccaa ttttcggcgg tgaactttac tcgttttagc cattaattac
    42721 gagctttaaa aaaactcatt caacggatgg attgaaggcg ggaagcgtcg atgtgaaaca
    42781 gacgggcgaa gttatcggtg gttacctgcg ccagttcttc aacggcaaca cctttcaaca
    42841 cagccatgta ttctgcaacg tcacgaacca tcgcaggttg attctctttt cctcgatgcg
    42901 gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa tcgatccagg gggacataac
    42961 gcgcagcatc gcacagttgc tccgcattac ggaaggtcac aatgccggaa aaggagatgt
    43021 aaaatccgag atccagtaat ttacccgccg tttctctgtc ctctgtaaaa cagtgtagta
    43081 cgccaccgca atccgtcact ttttcttcgc gcagaattgc cagcgtatcg gcacgggcgt
    43141 cacgggtatg gacgataacc ggcttgttca gttcacgacc aatctggata tgatggatga
    43201 aggactcttg ctgacgtact ttagtttccg gtgtgtaata ataatccagc ccggtttcac
    43261 ccagcgctac aacaccctct tctgccgcca gacggcgtaa atcttctaca tcgtagggat
    43321 cattctggtt gagtggatgc acgccacaag aaaatacgac gttgtcacgt tcgcctacca
    43381 gatcccgcat atgtaaataa cccggtaatg ttgtggcgac tgccagacaa aatttcacat
    43441 cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat tcataatcca
    43501 gaccatcgag atggcagtgt gagtcgacta aaaacatgat gtctctctta aagatgagga
    43561 accggtagca caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag atcggtgatg
    43621 agaagctcgc ggttgatgcc tgtgacagac attaactgtt cacgaatgtg gcaaacatcc
    43681 cccagtatag cctgcaggcg cgagggagaa agatggtttg ccagttcggc gaccaggccc
    43741 ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc gttttagcgc atccatcagc
    43801 aacgttgcca cccagtgtaa acgcgccgga gcttgttcat gattaagggc cgccagcagc
    43861 gaataccagt cgcccgatgg cacgctatat gccaacgcct gacacaatgt ttcacgagcc
    43921 tgccagttat ctccctgaaa caacgccagt gccgcgccag gcgaaccggc gcttaagcgc
    43981 aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca agtcacggcg
    44041 tactgttctg gaggcggcgc aaggtaatgt aaccgacaac gactacgtaa tgttgccagt
    44101 aaacgttcag gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg ctcttcaagc
    44161 gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt tacccagaca
    44221 actttcgcac cacctaggcg tgcgtgctca ttcagctttt cggtgacctc acgtaccgca
    44281 tcaatgccca gcgtattttt ccctttttcg ggagccaggg tgtagtaatc gggatgcgtg
    44341 ccagcctgca tcaactgaca tccacgacag tgaccgcaac ttttgtggcc ctgcggttgt
    44401 tggcagagta aataacggct cagggcgtag attaaagcat catcgcccat gcccggtaac
    44461 gcctgaatga gtagcgcatg gtgacctctt ccggcctgat agctggctac cagtttttcg
    44521 aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa ctccttcacc cagtgggtca
    44581 cggtagtgcg gattgcatcc atcacggcct ccagcggctg ggtggcatca atggtatgaa
    44641 tacttttatc ttgtgcagcc agttccagat agcgggcgcg ggtgcgatta aagaaatcga
    44701 aagactcttg ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt aagccaactt
    44761 ccggggtaac atcgagatag agcgttaagt cggggcgaaa atccccgaga acagcatcac
    44821 gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc gccctgatac gcctgagtgg
    44881 agagatcgtg gcgatcgcca atcacccagg tgccgttagc cagcgcaggt ttgatgaccg
    44941 tttctaccag ttgaacgcgc gcggcataaa acatcagaac ttcggcttta tcggtaatga
    45001 cttcatcgcc taccgatttg atatccagca ccaggcttct taacttttcg gcaagttgcg
    45061 taccgccagg ttcccgagtg aaaaccatgt cgcggatacc cagttgctcg agcgtctcaa
    45121 ccaccacatt acgcgcggta gttttgcctg cgccttccag cccctcaatg acgatatact
    45181 tactgcgcat tttttcctta agcactttca gataatcctg cacagacttg ttatgactgg
    45241 caagattggt attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg
    45301 tttttgccgg atgcgcagca gccttcagcg aatccgcccc cggcgtagct atcgcacccg
    45361 gcggcaaacc ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag
    45421 aaagtttgcc attataacgc tctcccatcc cgtaaatcac ggttgggtcg gtctgcaggc
    45481 gcataccaat gcgtaaacgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa
    45541 cggcggtttc tttttcgata attgatgcca tcgtcaccag ctgattttta tctttataag
    45601 gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgag
    45661 ctcgcttgag taacgcgaca tcggtggtat tggcggtata catcaaggtg tctggccaga
    45721 accaaccttc aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact
    45781 tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg caattgcttg aggtagtcgc
    45841 tcagacgcat cccttctacc agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca
    45901 atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt
    45961 gagaaagatc cggttcgata cgcagcagcc attgaaaaac ccgtgggcga ttgatgatct
    46021 tatcagcata aagctgttca ccgagcgcca gacgtccggt ccctggcttc agggtaaata
    46081 tcgtctcttc tttgataagc aatttgctgt cggcaagatg gcgaaccttc cagacgccca
    46141 caccagcggc gatacccagt accaccagca ataacaagat aatcaataac acttttttca
    46201 tgactaattc gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa
    46261 ggtgacatcg ccacaggcac gtacgggcat cactggcatt aacgcattac aaataaccat
    46321 ctcatctgcc tgcaacgcct cctccagcga ggcttgcact tcgacaagct gataagagga
    46381 ttgtgccagt aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag
    46441 tcgcggcgta taaactacat tgcccttccg ccagaacaaa ttagccgcac agcattccgt
    46501 aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga
    46561 gcgaatcaat acttgctcaa gccgattaag atgtttaata cctgcaagat gaggattgcg
    46621 ccccagccgc accgggctta gcgccaacgt catcccttcg ttacgcaaac ggtcgtaatg
    46681 tgcaggataa gccgtaacgg agagaatccg cgttgctggt ccgctgttca atgtgctgta
    46741 ccctcgcccg ccactaccgc gactgatcac gactttcagc acaccatttt gctgttctgc
    46801 tgccagcgtt ttcatctctt gttcaagctg aggccagaag tcacaggaaa tcatcaaccg
    46861 ctgacaagca tcctgtagtc gctggatatg cgccgataac aaactgactt taccgtcgat
    46921 aactctggcg gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag
    46981 cgattcctgc ttataaccgt taattaagaa cataccggct ccttatcaga gaattgttag
    47041 tgtggcagca tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa
    47101 gcggaccttt tatatgggtg gaaaatgaca acttagatct ttttaaagat caaagaacca
    47161 ttagtgccac cgaagccgaa ggagttacac agcgtgtatt ccattccgct gacctgacgt
    47221 gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt
    47281 ggcggaacag cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc
    47341 gcacctaaca ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct
    47401 tcaccgaaga tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta
    47461 ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt
    47521 gccattgcca gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg
    47581 ctgctcatac caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt
    47641 tcgtactctt caagtaccag cataccggca ccatcgccca gtacgaagcc atcacgctct
    47701 ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc
    47761 gcgccaaaac caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc
    47821 acgtcagcat cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa
    47881 gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga
    47941 cctgccacca tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca
    48001 ccgttcatca gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca
    48061 attgcggcac caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg
    48121 gcctgaacgc cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg
    48181 cgcgagataa tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag
    48241 gcgctagtat cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc
    48301 caggtagact ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca
    48361 cgacgcttag acacgtttgt cctccaagga gggaaaaatg attctagtgg gacaaaaaga
    48421 taaaactcag gcggtcgaac gaccgcctgg agatgttcac ttacgcctgg tggccgttga
    48481 tgtaatcaat ggcagcctga acggtggtga ttttctcagc ttcttcgtcc ggaatctcag
    48541 tatcaaactc ttcttccaga gccattacca gctcaacggt gtcaagagaa tccgcgccca
    48601 ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg cttaacgccc agctgttcgc
    48661 cgataatttt cttaacgcgt tcttcgatag tgctcatact cttaaatttc ctatcaaaac
    48721 tcgctttcgc gatggttttc gtagtgtata aaatgttgaa aaatttgcaa ctaaatcccg
    48781 gcaggtctta ccacgatttt acgttatttt gaggccttac cccccaataa cgcaaatcat
    48841 tttgcactaa ttattactca taaccacgca gactgcgcgc aacatgagca ttttgtgcaa
    48901 atcgcggtca gaccatgtac atcccgccgt tcacatgcaa agtttcaccc gtgatgtaag
    48961 ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat ttcctgtgcg ccgccgaggc
    49021 gacccgcagg aacctgcgcc aggatacccg cacgctggtc atcgctcagc gcacgtgtca
    49081 tgtccgtttc aataaagccc ggagcaacaa cgtttacagt aataccgcgt gacgcaactt
    49141 cgcgcgccag tgatttactg aagccgatca agcccgcttt cgccgcagcg tagttggcct
    49201 gaccgccatt tcccatggta ccaaccacag aaccgatagt gataatacga ccatgacgct
    49261 ttttcatcat agcgcgcatt accgcttttg acagacggaa aacagatgaa aggttagttt
    49321 cgataatatc gttccactct tcatctttca ttcgcattaa caggttatca cgagtgatac
    49381 cggcattatt gaccaggata tccacttcac caaattctgc gcgaattttt tccagaacag
    49441 attcgataga tgccgggtcg gtcacattca acatcagacc tttgccgttg gcacctaaat
    49501 aatcactgat cgcctgagcg ccattttcac tggtcgcagt gccaataact ttcgcgccac
    49561 gggctgcgag cgtttcagca attgcgcggc caattccgcg gcttgcaccg gttaccagtg
    49621 cgatttttcc ttcaaaattc atgattttcc tcttttaaag ctcgagcgcc gctgccatcg
    49681 ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac aatgcgtttc gtcaggccag
    49741 taagcacctt gcccgggccg acttcataga gatgttctac gccttgcgct gccatgtact
    49801 caacagactt cgtccactga accgggttat acaactgacg taccagtgcg tcacggatgg
    49861 catcaccatt ggtttcgcat ttcacatcaa cgttattcac aacagaaact gttggtgcgt
    49921 taaaggtgat tttcgctaat tctactgcca gtttgtcggc tgctggtttc atcagcgcac
    49981 agtgagacgg tacgctcact ggtaacggca gcgcgcgttt tgcgccagca gctttacagg
    50041 cagcgccagc acgctcaacc gcttctttat gaccggcaat aaccacctgt cccggagagt
    50101 taaagtttac cggagaaacg acctgacctt ctgcagcttc ttcacacgct ttcgcaatag
    50161 acgcatcatc cagaccgatg attgccgcca tagcgcccgt gccttccggt acggcttctt
    50221 gcatgaactt accgcgcatc tcaaccaaac gcaccgcatc agcgaaatca atcacgccag
    50281 cgcaaaccag cgcggagtat tcccccaggc tgtgaccggc catcattgcc ggtgctttac
    50341 cgccctgctg ctgccatacg cgatacagcg caacagatgc agtcaacagc gctggctggg
    50401 tttgccaggt tttattcagt tcttcagctg gcccctgctg ggtcagcgcc cacaggtcgt
    50461 agcccagcgc cgcagaagct tcagcaaacg tttcttcgac aattggatag ctcgccgcca
    50521 tatcagccag cattccaacg gtttgagaac cctgtccagg gaacacaaat gcaaattgcg
    50581 tcatgtttta atccttatcc tagaaacgaa ccagcgcgga gccccaagtg aatccaccgc
    50641 caaaggcttc aagcagaacc aactgccccg gcttaatgcg cccgtcgcgt acagcttcat
    50701 ccagcgcgca cgggacagag gccgcagagg tattaccgtg gcgatccagc gtcaccacga
    50761 cattgtccat cgacataccg agttttttcg ccgttgcact gataatacgc aggttagcct
    50821 gatgcggaac cagccagtcc agttgagaac ggccaagatt attcgccgcc agcgtctcat
    50881 caacgatgtg cgccagttcc gttaccgcaa ccttgaagac ttcgttgccc gccatcgtca
    50941 gatgaattga attctctgga ttcacgcggt cggcgtttgg cagcgtcagc aattcaccat
    51001 aactaccgtc ggcatgcaga tgggtggaaa tgattcccgg ctcttcagag gcagccagca
    51061 ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc acgatctgtt ggatcgcagg
    51121 tgcgcgccag tacatcggaa ccgacgacca gagcatactt caccgcccca gatttcacgt
    51181 attgatcggc tacgcttaat gcataggtga aacctgcgca ggctgctgca acgtcaaatg
    51241 ccgggcaacc tttaatgccc aacatgcttt gaatctgaca agctgcgctc gggaaagcgt
    51301 gcgtagcaga agtcgttgcc acaacgatca ggccaatctg gtctttctca atgcccgcca
    51361 tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga aacggtttcg tttggcgcgg
    51421 caatgtggcg ttcgcggata ccggtacgag tgacaatcca ctcgtcagag gtgtccacca
    51481 ttttttccaa atcggcgttt gtccgcactt gttcgggcag atagctgcca gtaccaataa
    51541 tcttcgtata catgtacgct cagtcacttt tcggttatat accgtcactt gcaaactgcg
    51601 agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt tgccaccgtc cagcagctca
    51661 aaaccagctg ggtatacaga ttccaggcga gcggcaattc gctgaggaac ttgtcgctgc
    51721 accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc gctgattggc tgcaccatga
    51781 ctttttatca ccgtgccgcg caatcctaac agacaggcgc cgttatactg gtcggggttg
    51841 aggtgactga atcgcctcgt caggctcttt tgtagccaac gctttaataa cagtagccac
    51901 cacgaccgtt ttttcccttc accctgagat ttcagcagag aaaggaacat cctgacaaca
    51961 ccttccatcg tctttaacgt gacatttcct gtaaagccgt cacaaaccag cacatctgtc
    52021 ttgccagtta acaactcatt ggcttcaaga tagccgatat aattgataga agggattgtt
    52081 ttaagcaccg ctgaggcatc ccgaatactg tcaagaccct ttacttcttc ttcaccaata
    52141 ttgagcaacg ccacgcgagg attgggaatt tccaccacct cttcagccag aactgagccc
    52201 ataatggcaa attgcaccaa cattgtgctg tcacaatcga cgttggcccc taaatcgagg
    52261 accaccgttt tgcccttttg ctgatgtggt aataccgtca ccagcgccgg acgctcaatc
    52321 ccctccaggg gcttgagtaa taattttgcc agccccatca gtgccccggt attaccggca
    52381 ctgacacagg cttgcgctcg accttctttc accagctcca gggccatacg cattgaactc
    52441 ccacgactgg cgcggatagc ttgcgaaggc cgggcatcac tggcgataac tgactgcgca
    52501 ggaataatct gcagacgcga acgttgttca aagtcagctt tagcaagtaa tggcgtgatg
    52561 gcgtcgggat tgccgactaa aagaagagtg agttgcgaat tagaattcag tgcctgcaat
    52621 gctgcaggca ctgtcacgga agggccaaaa tcccctccca tgacatctaa cgccagggtt
    52681 agacgtgtca aggtatcgtc gccgcccggt ttggtctttc cccagttgcc tggggaaaat
    52741 cctcactaag cttcatcacg cagacgcgtg attacttagc gatgaccttg cggccgcggt
    52801 agtaaccgtc ggcagtgatg tggtgacgca ggtgtttttc accagaagtt ttgtctacag
    52861 acaggctggt gactgcggtc agcgcgtcat gggaacgacg catgccacgt ttggaacggg
    52921 ttggtttatt ctgttgtacg gccatggacc ttactcctca attacggttt atccccgatc
    52981 caacggggag caccaattac ttacgcttta agctggctaa tacggcaaat gggtttggct
    53041 tttgcacttc ttcaggcagt tcaccaaaga ccatgtccgc ttcggacact tcacagtgtt
    53101 cagaatcatg caccggaact accggcaagg cgaggatgat ttcatcttca accattgcaa
    53161 gcagatcgat ttcaccgaat tcgttaacct caatcggttc atacgcttcc ggcagtgctt
    53221 cagcctgttc gtctgaacgc acaggactaa aacaatacgt tgtgtagacc tgatgagtaa
    53281 acggcttccc gcaacgctga cactcgagcg ttaccgtcac cttcgcatcg ccgtttaaca
    53341 ctgcgagacg ttggttatcg atagcgaacg acatggagca ttccacatca ctgtccacac
    53401 tgactacgga ttcggcgacg cgctcaacct gatcaggggt atagataccc tggtaatcaa
    53461 ggcgtttttg agccgtacga accggatcga gagtcagggg gaattttacc ttttgcatag
    53521 ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa aaaggcagcc tgtggttgcc
    53581 ttttgccaat aattcgcaca cattgcgggt tactgcttta ttttcgttca gtgctgataa
    53641 cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg gctatcatca atacgctata
    53701 tctgatggaa aaaaatatgc ctaaacttat tttagcctcc acctcgccat ggcgtcgcgc
    53761 tcttctggaa aaactgcaaa tctcttttga atgtgcagca cctgaggtcg acgaaacccc
    53821 acgcagcgac gaatcaccgc gacagttggt gcttcgactg gcacaagaaa aagcgcaatc
    53881 tctggcgtca cgttatccgg atcatttaat tattggttca gaccaggtat gtgttcttga
    53941 tggcgaaatc accggtaaac cgttaacgga agaaaatgcc cgtctgcaat tacgcaaagc
    54001 cagcggcaat atcgtcacct tctatactgg actggcgttg tttaattcgg cgaatgggca
    54061 tctacaaaca gaagtggagc cttttgacgt ccatttccgt cacctaagcg aggcggagat
    54121 tgataattac gtgcgtaagg agcatcccct gcactgcgcg ggtagcttta agagtgaagg
    54181 atttggcatt acgctgtttg agcgtttaga gggacgtgac cctaacacgc tggttggttt
    54241 accgctaatc gcgctgtgtc agatgttacg tcgggaaggg aaaaacccgc tgatgggata
    54301 gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc ggtgcaggcg gcctgatgcg
    54361 gcgtaaacgc cttatcaggc caaccgccca ttatcaggct tatatgcttg tattagcgcg
    54421 cgttacgcag tttttgcaaa caacgcttca aaccttcatc catcggcgct tcgatacgca
    54481 tcacctcacc ggtccccgga tgggtaaact tcaacgctgc ggcgtgcagg aacagacgat
    54541 ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc acggtcaccg tagcgatcgt
    54601 caaaggcaat cggatgaccc gcatactgtg tatgcacacg gatctgatga gtacgtccgg
    54661 ttaccgggct acaacgcacc agggtggcaa atgcatagcg ttcttccact ttaaagcgtg
    54721 tttccgacgg tttgccttcc tgactgacac gcacgatacg ttcgccgctt tgcagaatat
    54781 ttttcaataa cggcgcttga acgctcttca catgcgactg ccactgaccg cgcaccagcg
    54841 ccaggtaatc tttttgcatt cctttttcac gtaattgctc atgcagagaa cgcaacgccg
    54901 agcgtttttt cgctaccagc aacacacctg aagtgtcccg gtcgagacga tgaaccagtt
    54961 caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc gataacgccg aagcttaaac
    55021 cactgccgcc atgtaccgcc gtaccggaag gtttattcag caccaggata tgatcatctt
    55081 catataagat gacgtccgcc agcgccgcca ccttttgcag atgtggcgaa accgcctctt
    55141 cttcccgctc agcaacgcga accggtggga tacgcacctc atcacccgct tcgagtttat
    55201 attcaggctt aatacgtttt ttgttcaccc gcacttcacc tttacgcaaa atacggtaaa
    55261 tcatactttt tggtacgcct ttcaattggg tacgcaaaaa gttatcgata cgttgccccg
    55321 cttcgtcagc ggtgatagca acaattttta cggatggagt ctctgttttc atggtgggcg
    55381 attctaatta gccaacagga ttcgcgccac tcatttttct atgcttatat ttagttttgc
    55441 accttattac ttcactacgt gatcacttta ttgatggtta ttaaaccaat caccagcaag
    55501 aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa agtcatcttg ctataacaag
    55561 gcttgcagtg gaataatgag gccgtttccg tgtccatcct tgttaaaaca agaaatttta
    55621 cggaataacc cattttgccc gaccgatcat ccacgcagca atggcgtaag acgtattgat
    55681 ctttcaggca gttagcgggc tgcgggttgc agtccttacc ggtagatgga aatatttctg
    55741 gagagtaata cccagtctgt ttctttgata attgcgctgt ttttccgcat gaaaaacggg
    55801 caaccgacac tctgcgcctc tttgagctga cgataaccgt gaggttggcg acgcgactag
    55861 acacgaggcc atcggttcac acccggaaag gcgttacttt gcccgcagct tagtcgtcaa
    55921 tgtaagaata atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac tcagcaggaa
    55981 gagttgcgcg ttgcccttgt agatgggcag cgtctgtatg acctggatat cgaaagtcca
    56041 gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat tgaaccgagt
    56101 ctggaagctg cttttgttga ttacggcgct gaacgtcacg gtttcctccc actaaaagaa
    56161 attgcccgcg aatatttccc tgctaactac agtgctcatg gtcgtcccaa cattaaagat
    56221 gtgttgcgtg aaggtcagga agtcattgtt cagatcgata aagaagagcg cggcaacaaa
    56281 ggcgcggcat taaccacctt tatcagtctg gcgggtagct atctggttct gatgccaaat
    56341 aacccgcgcg caggtggcat ttctcgccgt atcgaaggcg atgaccgtac cgaattaaaa
    56401 gaagcactgg caagccttga actgccggaa ggcatggggc ttatcgtgcg caccgctggc
    56461 gtaggcaaat ctgctgaggc gctgcaatgg gatttaagct tccgcctgaa acactgggaa
    56521 gccatcaaaa aagccgctga aagccgcccg gctccgttcc tgattcatca ggagagcaac
    56581 gtaatcgttc gcgccttccg cgattattta cgtcaggata tcggcgaaat ccttatcgat
    56641 aacccgaaag tcctcgaact ggcacgtcag catatcgctg cattaggtcg cccggatttc
    56701 agcagcaaaa tcaaactgta caccggcgag atcccgctgt tcagccacta ccagatcgag
    56761 tcgcagatcg agtccgcctt ccagcgtgaa gtccgtctgc catccggtgg ttccattgtt
    56821 atcgacagca ccgaagcgtt aacagccatc gacatcaact ccgcacgcgc gacccgcggc
    56881 ggcgatatcg aagaaactgc gtttaacacc aacctcgaag ccgccgatga gattgctcgt
    56941 cagctgcgcc tgcgtgacct cggcggcctg attgttatcg acttcatcga catgacgcca
    57001 gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca ggaccgtgcg
    57061 cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg ccagcgcctg
    57121 agcccatcac tgggtgaatc cagccatcac gtctgcccgc gctgctccgg taccggtacc
    57181 gtgcgtgaca acgaatcgct gtcgctctct attctgcgtt tgatcgaaga agaagcgctg
    57241 aaagagaaca cccaggaagt tcacgccatt gttcctgtgc caatcgcttc ttatctgctg
    57301 aatgaaaaac gttctgcggt gaatgcgatt gaaacgcgtc aggacggtgt tcgctgcgtg
    57361 attgtgccaa acgatcagat ggaaaccccg cactaccacg tgttgcgcgt gcgcaaaggg
    57421 gaagaaacgc cgaccttaag ctacatgctg ccgaagctgc atgaagaagc gatggcgctg
    57481 ccgtctgaag aagagttcgc tgaacgtaag cgtcctgaac aacctgcact ggcaaccttt
    57541 gccatgccgg atgtgccgcc agcgccaacc ccagctgaac ctgccgcgac tgtcgtagcc
    57601 ccagcaccga aagctgcaac ggcaacacca gcagctcctg cacaacctgg gctgttaagc
    57661 cgcttcttcg gcgcactgaa agcgctgttc agcggtggtg aagaagccaa accgaccgag
    57721 caaccaacac cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg caaacctcgt
    57781 cagagcaacc gccgtgaccg taatgagcgc cgcgacaccc gtagtgaacg tactgaaggc
    57841 aacgataatc gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca aactgccgag
    57901 acgcgtgaga gccgtcagca ggctgaggta acggaaaaag cacgtaccac cgacgagcag
    57961 caagcgccgc gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca ggcgcaacaa
    58021 gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt
    58081 gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt gcgttacgag
    58141 caaagcgtag ccgaagaagc ggtagtcgca ccggtggttg aagaaactgc cgctgccgaa
    58201 ccaattgttc aggaagctcc agctccacgc acagaactgg tgaaagtccc gctgccagtc
    58261 gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg ctgataaccg tgacaacggt
    58321 ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc gcgtgagtgg tcagcgtcgt
    58381 cgtcgctatc gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc
    58441 gcgtctccgg aactggcctc tggcaaagtc tggatccgct atccaattgt acgtccgcaa
    58501 gatgtacaag ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc gatggtgact
    58561 gaggtccctg tcgccgccgc tgtcgaacct gttgttagcg cgccagttgt tgaagaaatg
    58621 gctgaagtcg tagaagcccc cgtaccggtt gccgaaccgc aaccggaagt ggttgaaacg
    58681 acgcatcctg aagtgattgc cgccgcggta actgaacagc cgcaggtgat taccgagtct
    58741 gatgttgccg tagcccagga agtcgcagaa cacgcagaac cggtggttga accgcaggaa
    58801 gagacggcag atattgaaga agttgccgaa actgctgagg ttgtggttgc tgaacctgaa
    58861 gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt tgaaacggta
    58921 gccgcggttg aaccagagat caccgttgag cataaccacg ctaccgcgcc aatgacgcgc
    58981 gctccggcac cggaatatgt tccggaggca ccgcgtcaca gtgactggca gcgccctact
    59041 tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc
    59101 gctcctgcgc gtccgcaacc tgttgagtaa taattagctc aaagtaatca agccctggta
    59161 actgccaggg cttttttatt tcatgtttga atcaacatat taaaattcat caaatggcat
    59221 ccttgctaac caacaatgca aaataggcaa aaaaaatgcc cgccccggcg atcccagagc
    59281 gggcagacaa aacataccca gtttcatgat atgttttaaa gcgagcttat ttgttgagct
    59341 ggaagagcga caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt
    59401 gctgcatgat gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct
    59461 gctgcgtttg ccctaaagcg cgatcgctac ctaaggaatc cagcgactcc agtttgttca
    59521 gctgcgtgcc taattccgtg cgaacggtaa ggacattatt cagcgagttt ttcagtccac
    59581 ggttggtttt atccaatgcc gcagcggcga tttctttatc cgcttcgcta tccgcgaccg
    59641 gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc
    59701 taccgtctgg ttccgctacc gcgttactgg taatactgtc gaaaattttg tcgcccgtat
    59761 gtcctatcac catcgaacgc gaagcatcga cctgttgctt aatactttct gttccgccga
    59821 cataatcacc gttagcctca ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt
    59881 agcgcccatt accgtcagtg gtgttcgcca gattcagcaa ctggtcacga agcccctgaa
    59941 tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt gccattgctg gcgtagacaa
    60001 ttttttcctg agcattctgg atagcagtgg tgacctggct aagtacactc tcttccagtg
    60061 acactttttg agtggcgaaa gtacgtgcca gcgtgtactg gctgttttgc gcctgtgcct
    60121 gggagagaac aacagcttgt gatgcagcaa tgggatcgtc tgaagggtta acgactcgct
    60181 tacccgtcga catctgctcg ccgtacttca tccattctgc ctgagaattg gtgatgccgc
    60241 gcatgttttg ctggtacatc atctgggtac tgaaacgcat tttatctccc cttagcgaat
    60301 gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta
    60361 atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac cggaaatcga
    60421 ctgctgctga ttggaaagct gcgtcaccac attaccttgc gtggtgctac tggttttcaa
    60481 cgtcgcggtt ttattaccga tatcactcac taacgaggca taagcgtcgt taaaggattt
    60541 cgcaccgccc accgttttac tgttgctttg cagatccagc agggcctgac cgttgcggtt
    60601 gtcgctatca cccgcatctt cttcgctcgc catcgcgatt ttcgcttcgt cggtgattaa
    60661 tacatccatg ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt cgttaacggc
    60721 aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatctgg
    60781 tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc tgccactgat tattatcgaa
    60841 cgagattttg taatctgtcg ccagtaccac ggaggcatca gttaccgtgg caccgatcgc
    60901 aacgtcacct ttgtttttcg tgttttgctg aaccgcgggc ttaccgatag taaagaaatc
    60961 ttcaccagca tcgccgttag catcaaatcc ggctttgtgt tggctgttga aagcctcggc
    61021 aaatgccagc gccagttgtc caagcgtatt acgcgtctgg tccagatcct gagaacggaa
    61081 tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc
    61141 tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc
    61201 cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa ccattggcat cgtgatgtta
    61261 taagtaccgc catcctgaac gctgacttct acgccaacaa tctggtttaa ttcgctcacc
    61321 agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg
    61381 cgcgagattt gatcgttcag gctggcaatt tgtttagcgt aattgttgat ctgatcaacg
    61441 ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gcagatattg atcggtggtt
    61501 ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc cgccgggtct
    61561 tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc
    61621 gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata gcgggcggtc
    61681 aggccgctac tttgcgtctg cacagcacgt aactggttgg taataaacgt atcatactca
    61741 cgctgcacac cagaaacgta gacgccattg ccaacccagc cgccagcgcc caacgtgcta
    61801 ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata
    61861 ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttattg
    61921 atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcga
    61981 cccgccggac ttgagttatt cagaacagat tatcaatgtt catgctgtag gttttgctca
    62041 cctggtcgct tatcgatttc atctgctgga tcatgttggt gagtttacgg gcatagtgag
    62101 gatcggtagc atagcccgcg tcctgtagga cctgcgcccc ctgttccgca ctcgcggcgg
    62161 tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc aacgtaatcc gacaatgctt
    62221 ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg ccgttttcat
    62281 attcagtcgt ggtgatttca gtgacttgcc ctttccagtt gccagaggct ttgacaccaa
    62341 acaggttata gcttggctcg ccgttttcgc ggcggatttg ccgttgcccc caaccagatt
    62401 caagcgcagc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt
    62461 gggcgggcag cgagagttgc gcgaggaatg ctctactgtc acccggcagc gaatcatcgt
    62521 agttacgtgg tacggccttt tgcaccagct gcgaaagcgt ctggttttga taacgcacca
    62581 cggtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc aatggttgtt
    62641 ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc gccgtcatct
    62701 gttgggcaat ttgctggtca tacatactgg tatacagacg agtgtgctcg ctgctgaaca
    62761 ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt
    62821 ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgctcgct ttcgccttta
    62881 gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgctg tcgctgatca
    62941 tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt gcaaaataga
    63001 catcagatcc atcggcgtag cgcccagcgc attgagcgca cgcaccacgt tattgaggct
    63061 ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg
    63121 tggagtaaca acagtctgtc caccaccaaa cggtgtatct ggctggctga cattggcctg
    63181 acgattaact gttactgaga gatttccctg cgctaccgcg cagctgtcga gggtcacttc
    63241 tcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt cctgcggggt
    63301 gacattaacc tgcatattct ggatatcggc aaggaaacgg acctgggaac tgttgccact
    63361 tggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat atccacgcac
    63421 gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa
    63481 attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt
    63541 ggtgatccgt ccaccgttca gttggttaac ctgaacactg ctaccgcccg cggaggctcc
    63601 tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt
    63661 aagcggtgtc atcaacaacg tacctccacg caagcttttg gcattaccca tggaagaaac
    63721 caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgacgctg tcaccattac
    63781 cacagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc ccagctgtga
    63841 gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt caccagtgcc
    63901 atccagcccc actaccaggc catagccaat cagtgagttt tgccttaccc cctgaacact
    63961 ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta accagtagaa gaattaatgc
    64021 agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag gaagaaacgc
    64081 tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatacgc
    64141 gcatccgcca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg tggattaacc
    64201 acgcccgaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt ttcacccacc
    64261 acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag
    64321 gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga ggcttcgaca
    64381 tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa
    64441 ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc
    64501 tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt
    64561 tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc
    64621 ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga gggtatccag
    64681 gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt
    64741 ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac cggttatgcc
    64801 ttagagttgc gtcagttttt gcagcatctg atcggtggtg gacaccgctt tactgttgat
    64861 ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga
    64921 cgtttcaaca tacccttgat acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt
    64981 cggtgcaccg gaggattgcg tttcggtgta gaggttttcg ccaatgctct ccagtccggt
    65041 atcattcata aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg
    65101 ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat tcgccggaat
    65161 ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc agctgcccgt tctgatccac
    65221 ctggaaagag ccgtcacggg tataggctga tgagccatct ggcaacatta cctggaaaaa
    65281 gccctgccct ttaatcgcga catctttgct gttattggtc tgcgacagat tgccctggct
    65341 gtgtaagcgt tcagtggcgg ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa
    65401 ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc
    65461 aaacaccgcg cgctgacgct taaaaccgtt agtactgacg tttgccaggt tgttggcaat
    65521 gacgtccata ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat
    65581 catataatgt cctgtagtgg attgctgaca agcagagcag aagcacgcga cagtgtgaat
    65641 ttcccctcac cctaaccctc tccccaaagg ggcgagggaa tagtccgcgc gcttttattg
    65701 caatgtcatc aatcgttcaa ttaactcatc gacagcagtt ggttggcacg gcctgcgtta
    65761 tcatcgacgc tgctgatcac cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg
    65821 tcgctcattg ccgcaacggc attgacgtta ctgccttcca gaacccccga catcacacgc
    65881 aaggttggat ctgcctgcag taccggccct cgcgtggcct gggtttctgc gcttaaacga
    65941 aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag tttcagacgc
    66001 cctactggcg caactgtatt tgccggatcg cccggattga gcgccgagat tgtgccatcg
    66061 gcagcaatcg tgatttccgc cccttccggc acagcaattg gccccgcctc gcctatcacc
    66121 ggatgcccct gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc attacgcgta
    66181 tacccttcgc tgccgtcagc ggtctgcacg gccagccatc ctgctgcaac gcaacgtcca
    66241 gcgggcgcga ggtgtaatcc atcttgccgg gcgtcatgtc tgcacccggt gttgacgccg
    66301 ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg cactgcgcgc aaggcgttta
    66361 actgcgcgcg aaaaccgggc gtagaggcat tggccagatt actggcggtt accgcctgtt
    66421 gattcagcgt ctggctggct gctcccatcg cagtataaat tgcgtgatcc attgagctat
    66481 cccgtcagcg attagcgtaa gttaacccgc gtgttgagga tctggtcctg ggttttgatg
    66541 gtctgggcgt tagactgata gttacgctgg gcaacgatca tattgaccag ttctttactg
    66601 agatcgacgt tggacgcttc cagcgcaccg ttggtcaggg tgccaaagtt tcccgtcccg
    66661 gctgtcccca acagcgccac gccagaagat tgcgtcgcag accagacgtt gtcgccttcg
    66721 gatgccagac cttcgttgtt ggcaaagttc gccagtacaa tctgccccag cagttgggtt
    66781 tgttcgttgg aatagttgcc gacaaccgta ccgtcatcat tgatttgata actcaccaga
    66841 tcgcccggtt tgtagccatt ctgggtggtt gccacaatgt tgttagcgcc ggtattttgc
    66901 tgcatggagt tgaggaagct cagactaaac gtggcgggtt ctgcaccgtt aattgcgccg
    66961 gttgcgatat tattcgccat cgcaccatcc actaatgtgc cattagcatt aaattccagt
    67021 gttgtcgctg tcttcgcaat gctgtttgga tcactgctat cctgggtgta gacctgccag
    67081 ttattatccc cggtcttcac aaaatagacg ctcatgtcat gagcattacc ctgactgtcg
    67141 aaaacagtca ccgaaccttt tttgttatag ctatccgcat tgctggcgct gaatggcgta
    67201 acagtaggaa gcggatcact ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt
    67261 ttcgctgcca tcagggtatt cgggatcgaa atattggtcg gattcgcccc ttgctgaata
    67321 gtcggcggcg taccggttgc cgggtaaccc gtcagctgta aaccttgcat attcaacagg
    67381 ttacggtttt catccagctt aaattgtccg ttacggctgt agaacaccga accgttgctg
    67441 tctaccagac ggaaaaaacc gttctggctg atagcaacgt ccagacctcg cccggtgttg
    67501 gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa cttttacccc cagtcccact
    67561 ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg
    67621 gagttggcga tattgttgcc aataacatcg aggttggtgg cggcagcgtt taatccgcta
    67681 accgcttgag aaaaggccat gactgactcc tgaaatgtga aggctcaaat tatctgccgt
    67741 acttcgtcga gggtggtggt gccgtaagtg ccgagatcca gcgtattacc gctgttgccg
    67801 cggatcacac cctgcaccag agcaaactgc agcggctggg caaccagttg tgtaccaccg
    67861 ttactggcgc taatcgctac attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac
    67921 gtaccgtccc aggtgaaact gtgaactccg gcggtcagtt caccaatatc aatggtgcgc
    67981 acaaccgcgc catttttatc ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc
    68041 tcaacaccaa acggcgtggt cgtggtcaca gccccttctt cactgccggt tcccgcaaga
    68101 acagttgtgc cggggatcat cacgccgtga ccgatcaggt tactggcctg taacgactgg
    68161 ctgttatcaa tctgtccgga aatagatccg agcgtggtat tgagtttttc aatcccgctg
    68221 accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt tttccattgg attggtcggg
    68281 tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg
    68341 ttgctgcccg tgagcgaact actgctggtg gtactgacgc cggtatttgt cggatcggtg
    68401 gtggttaccg caatggacat agctttctcc tttattgacc gagcgtaagg gttttcagca
    68461 tcatgctttt caccgtgttg agcacttcaa cattggcctg atagctgcgt gacgccgaca
    68521 tggtgttaac catctccccg acgacatcaa cgttcggcat ttttacgtag ccctttgcat
    68581 ctgccagcgg attacctggt tcataaacca gtttgtccgg ggcctgactt tcttaacatc
    68641 ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg ttaacctgga ataccacctg
    68701 ttttgcccga tatggctgtc catcaggacc ggtcacgcta tcagcatttg ccagattact
    68761 ggccgccacg ttcaggcgct gggactgggc agttaacgcc gacccggcga tatcaaaaat
    68821 attcagcagt gccatccgtt aatttccgct ctgtaaaacg ttcatcatgc ctttgatttg
    68881 cccgctcaac gcgctaaggc tcatctggta ttgcaggctg ttatcggcaa actgggtgac
    68941 ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag
    69001 cttatcgagc atatctcctc cgcaggtatc aaaattctgc catcagctta aatgccttta
    69061 cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct atttatgccg ttgatggtca
    69121 ttgcggacag gtacaactca cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa
    69181 acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta agtacggcga gcaatctcac
    69241 atcgcaattg cacaactttt ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt
    69301 ttctattcgt acagcgccca atctactacc gccatgcgag cagccattgc tttcgatgag
    69361 caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg
    69421 atatttacag gttaatgtgc aggccacagg aaattatgtg gttgccgcga tgcctattgt
    69481 gcggggagga aagctggaag ctggcaatgt caaactgaaa cgtggacgcc tggataccct
    69541 gccaccgcgt acggtgctgg atatcaatca acttgttgat gccgttagcc tgcgcgatct
    69601 atcacccgat caacctatcc agttaaccca gtttcgccag gcatggcggg taaaagcggg
    69661 acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc gccaacgcag aaggtcaggc
    69721 gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atggtatcgg gacaggtagt
    69781 cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg ttaaagatta
    69841 cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg aggaataaac
    69901 catgagtatt gatcgcactt cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac
    69961 cactgacgcg ccggtaacga acagccgggc ggcaaaaaca accgcctcca ccagcaccag
    70021 tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg atatcaatct
    70081 tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa tggacaccgg
    70141 caaaattgcc gatgcgctga tcaacgaagc gcagcaagac ttgcagagta actgatcgta
    70201 tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg
    70261 taatggatca tgagcagcaa catctctcta tggggcagat caactgcagc cagttgcaat
    70321 ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa cagttacgca
    70381 agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag gaaattactg
    70441 tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg gaaggacaga
    70501 ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg
    70561 gggcgaacgg tcagacctca acaccccatc gcggcagtaa aaagatttcg atctgaatat
    70621 tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg ggcaaattct
    70681 ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc cgccagcacg
    70741 accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc cggcatgata
    70801 tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag acgcaacaga
    70861 aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg ccagtaaagc
    70921 agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc atgtttcaac
    70981 ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat ggcaattttc
    71041 actggcgttt taatgtcttg gcgggaataa aagccaggag ccaacacttt cactacaatc
    71101 aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac
    71161 gcggtaaatt taccgtactg gaacagcgaa acggttaacg gacctgaaag aatgcccaac
    71221 gcaaccgcac tcggcagtgc caacaggaaa caaagacgca acccccagtc catcaaacgg
    71281 ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgacggcag caaaatggtg
    71341 ccaagcgcca cgcccagcac accggaggga aactccatta agcggtcggc gtaatacatc
    71401 caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat taaggagatc
    71461 tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg cattgctcca
    71521 gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag gtgcggtagc
    71581 tgatacacca gctgcaggat gccgccgacc gtaacagccc acgccagcgc cagcaccggt
    71641 gggttaaagt acggtgcggc aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt
    71701 ggcgcaaacg ccggaatcga gaagcggttc cacgtattca gaatcgctcc caccagcgac
    71761 gccagggaga tcagcaagat atagggaaag gtaatcttta gtagctggct ggtcagggca
    71821 aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca tgatcaccca cggtgcggcg
    71881 agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga aacataagag
    71941 acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat attccgccag aatcggtaca
    72001 aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg aagtttaaaa
    72061 gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac aattgcgtct
    72121 cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt
    72181 aataaattca tcggtgttct aatccggacc cacgggcaaa acgcctgcaa ggcaggcgct
    72241 gaaagaggcg aatagtctac ctagattatg gtgaattgct accgccagat gttacagggt
    72301 ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca gcactgccta
    72361 ttcgccggcg gtttgcggaa ctgtctgatt ttgcacgcat tcaatgaagt gccgcgcaca
    72421 gccgacaaat ccacgttgct caagagtgct ctgccaacca ggaatcggtt tattcactac
    72481 accctgcccg cgctcctcac gccattcgcg catatccgta atgtcgatga gcgcaccgtc
    72541 agtcacggcc tgcacggttt cacgctgact tccggcacgg cgatgcatac aagtggtgat
    72601 ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc atttcgccag cgtcgttagt
    72661 cagtagcgta ccgccatcca gagaggcttt gccgcccgac aaccacagcg cggtatccac
    72721 cacatgcaga taatcatcca gcaacgtgaa ataaagatcg tgtggcccga cgctattgct
    72781 acgatgttta tccattctta gcgaggctgc ggtggcgagt tgcgttttta actcaccgta
    72841 gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc gcgccgccag
    72901 ttccaccagc ctttcagcat cgcgcagatt ttctgccagc ggtttatcga cacagacatg
    72961 tacccccgca ttgagtaacg tactgaccac gtcaaagtgg ctggcggtgc tggagtgcac
    73021 aaaaaccgca tcacaactgg cggcaaggct ggataacgaa tcggcataag gaatgcgcca
    73081 gctgtcacaa attggcaggg ctttggcgcg cgtaggcgac taggctcctt gtaacgtcca
    73141 gtcagacgct gccgccagca ccggtaacca cgctttttgc gcaatgccgc ctaatcccac
    73201 tacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac gaatcaagac
    73261 gttgtttcag ttctgccact tcgatttcca gggcttcgac gcgggtctgt aaatcaccgt
    73321 caaccatgtt cgacatagcc gttaccgccg gctgatcttc aacctcacca ctgaaaagat
    73381 gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca aaaggaccat
    73441 cttcgcgatt cgccagttgt tccagcgtcg actccacttc cgccatatcg ctgaattcat
    73501 acattcgcgc ggcgcggctg cgcagttcac ccagcgtctg ggcaccacgc aataacaacg
    73561 tggtgattaa cgccacttct gcggcgctca gtttcagatc gccaaattct gaattacaaa
    73621 aacgttgctc atatttggtg acccgattac caaaaccgct cactgtgcgt agataatgac
    73681 gtttgaccag attatccagc tgttcctgca cttcggattc actcagattc atgaccggtt
    73741 cacggttcgt tttctgatta caggccgtga ctacaccatt gactgagagc aggtattgct
    73801 ccggcgtggt cacctgcttt tccagcaagc aaccaattac gcgagcttcc agggcagtaa
    73861 gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt agttaatgcc
    73921 gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc atagccttct
    73981 ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg catataatta
    74041 gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc ggtcagggct
    74101 tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag atagcaggta
    74161 tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc caataatctc tttttcgtcc
    74221 gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt aatcatccct
    74281 agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg ctcccagggc
    74341 ttgaggaaat ggcgattctc tgcgtaataa tccgccagac gccaggcatc acgatcatgc
    74401 accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt actgcgatag
    74461 ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa gtattactat
    74521 acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat tttctgcatt attcacatat
    74581 ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat
    74641 gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt cccgggaggg
    74701 gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat atttcctgct catcgataat
    74761 atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg cttcgttgat
    74821 caaatgggct gggccgccgt catggtcggt attgctctcg gtctacgcca gtttattcag
    74881 caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa accgatgatt
    74941 gttaccggta tgctgatgcg cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg
    75001 tggctattgt ggttttcatg cctgctctcg ggactcggcg gcacgttgtt tgatccgccg
    75061 cgttcggcgc tggtggtgaa actaatccgt ccacaacaac gtggtcgttt tttctcgctg
    75121 ttgatgatgc aggacagtgc cagtgcggtc attggcgcat tgttggggag ctggctgttg
    75181 caatacgact ttcgcctggt ctgcgccaca ggggccgttt tgtttgtgct atgtgcggcg
    75241 ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa
    75301 ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct atgtcctgac gctggcgggt
    75361 tactacatgc tggctgtaca agtgatgctg atgctgccaa ttatggtcaa cgacgtggct
    75421 ggcgcgccct ctgccgttaa atggatgtat gccattgaag cgtgtctgtc gttaacgttg
    75481 ctctacccta tcgcccgctg gagtgaaaag cattttcgtc tggaacaccg gttgatggct
    75541 gggctgttga taatgtcatt aagcatgatg ccggtgggca tggtcagcgg cctacaacaa
    75601 cttttcaccc tgatttgtct gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa
    75661 accttaagtg cttcgctggc ggacgcaaga gctcgcggca gctatatggg gtttagccgt
    75721 ctgggtctgg cgattggcgg cgctattggt tatatcggtg gtggctggct gtttgacctg
    75781 ggcaaatcga cgcaccagcc agagcttccg tggatgatgc tgggaattat tggcatcttc
    75841 actttccttg cgctgggttg gcagtttagt cagaaacgcg ccgcgcgtcg tttgctggaa
    75901 cgcgacgcct gatttgatac acccccctga ctcctccata ctgaaagtga actgacagcc
    75961 aaatggagga gtcacgtgaa gctatacatt tacgatcact gcccttactg cctcaaagcc
    76021 cgcatgattt tcggcctgaa aaatatcccc gtcgaattac atgttctgct caacgacgac
    76081 gcagaaacac ccacccggat ggtcggtcaa aaacaggttc ccattctgca aaaagatgac
    76141 agccgctata tgccagaaag catggatatc gttcactatg tcgataaact cgacggcaaa
    76201 ccgttactga ccggcaaacg ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc
    76261 tacgccaaca aactgctgtt gccgcgtttt gccaaatcgg catttgatga gttttctact
    76321 cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac
    76381 ctgctggccc actctgacgg tctgattaag aatatcagcg atgatttgcg tgcgctggac
    76441 aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt cggaagatga tattcagcta
    76501 tttccgctac tgcgtaatct gacgctggta gccggaatca actggccaag ccgcgttgct
    76561 gattaccgcg ataatatggc gaaacagaca caaatcaatt tgttatcatc aatggcgatt
    76621 taatcctgac ccgccgcggc agcctgtatt cgcgggttgc cgccgttttc agttttttct
    76681 gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt caggatattg acgcagattt
    76741 catgtcgagg actaccatga acaagttttt atttgccgct gcattgatcg tcagtggcct
    76801 gctcgttggc tgtaatcaac tcacccaata caccatcacc gaacaagaaa ttaaccagtc
    76861 gcttgcgaaa cataataatt tctcaaaaga tatcggttta cccggcgtgg ctgacgccca
    76921 tattgttctg acaaacttga ccagccaaat tggtcgcgaa gagccgaata aggttaccct
    76981 aaccggagac gccaatctgg acatgaactc cctgttcggt agtcagaaag cgaccatgaa
    77041 actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa ggtgcgatct tcctgaaaga
    77101 gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt
    77161 gcttccctat ttgaaccagg cattacgcaa ttactttaac cagcaacctg cttacgtcct
    77221 gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt
    77281 gaagccaggc gaaattgtca ttccgtttac ggattaatga cgagggcgca ttcgcgccct
    77341 ttatttttcg tgcaaaggaa aacatttccg cttatccttt gtgtccggca aaaacatccc
    77401 ttcagccgga gcatagagat taatgactgc accatcccag gtattaaaga tccgccgtcc
    77461 agacgactgg caccttcacc tccgcgatgg cgacatgtta caaactgtcg tgccgtatac
    77521 cagcgaaatt tatggacggg ctatcgtaat gcccaatctg gctccgcccg tgaccacagt
    77581 tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc gtacctgccg ggcacgattt
    77641 caccccattg atgacctgtt atttaacaga ttcgctggat cctaatgagc tggagcgcgg
    77701 atttaatgaa ggcgtgttca ccgctgcaaa actttacccg gcaaacgcaa ccactaactc
    77761 cagccacggc gtgacgtcaa ttgacgcaat catgccggta cttgagcgca tggaaaaaat
    77821 cggtatgccg ctactggtgc atggtgaagt gacacatgca gatatcgaca tttttgatcg
    77881 tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa
    77941 agtcgttttt gagcacatca ccaccaaaga tgctgccgac tatgtccgtg acggaaatga
    78001 acggctggct gccaccatca ctccgcagca tctgatgttt aaccgcaacc atatgctggt
    78061 tggtggcgtg cgtccgcacc tgtattgttt acccatcctc aaacgcaata ttcaccaaca
    78121 ggcattgcgt gaactggtcg ccagcggttt taatcgagta ttcctcggta cggattctgc
    78181 gccacataca cgtcatcgca aagagagcag ttgcggctgc gcgggctgct tcaacgcccc
    78241 aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg aatgctctgc agcactttga
    78301 agcattctgt tctgtaaacg gcccgcagtt ctatggcttg ccggtcaacg acacattcat
    78361 cgaactggta cgtgaagagc aacaggttgc tgaaagcatc gcactgactg atgacaccct
    78421 ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt aaacaataaa aatcgtagct
    78481 tcctgttgtc attaggttat tttacctgta taaataacca gtatattcaa cagggggcta
    78541 ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt gccagctggg gctattgacg
    78601 ccctggctgg cgaactttcc cgccgtattc agtatgcgtt tcctgataat gaaggccacg
    78661 tatcggtacg ttatgccgca gcgaataatt tatcggttat tggcgcaaca aaagaagata
    78721 aacagcgcat tagcgaaatt ccccaggaaa cgtgggaaag cgccgatgac tggtttgtca
    78781 gcgaataata tgcagtgatt tttgtttttg ccgggtcgcc ccggcttttt tattttactt
    78841 acgaattttg agtacaaaat ttctacactt cctcaaaata attgcacaat atgctaaaaa
    78901 aatatgaatg acatgctgtt ttattgttca tataaatctt aaaagcagaa atttatctgt
    78961 tccccccttt aaaaattcgg tgaataccct tacttattgg tatactgaag ttgcttcaga
    79021 taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg ggccatgatg
    79081 gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat cagcaccgtt
    79141 gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa gtccgagcag
    79201 gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag acaatttatt
    79261 tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt aaacgagtat
    79321 cggcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt gcctttacca
    79381 tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga gagcagagag
    79441 aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc tgccgccggg
    79501 tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg acgcccatat gccaccgcat
    79561 caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg tgaaggcgaa
    79621 aagtatgtcc cgttagtcct ccgcgcgcaa acgctgtggg atgaactttc ccgccacaac
    79681 gaagatgatc ccatttttgt acgctctggt gtcattaacc tcggcccggc tgactccgca
    79741 tttctcgcca acgtcgccca cagcgccgaa cagtggcaac tcaacgttga aaagctcgat
    79801 gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta catcggctta
    79861 tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg gatccaactg
    79921 gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg tcaccgcaat tcgtcatgac
    79981 gatgatggcg taactattga aacggttgac ggtgagtatc aggcgaaaaa agcgattgtc
    80041 tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc cgtacgtaaa
    80101 gtatttgcct ggtatcaggc cgatggccgc tatagcgtga agaataaatt cccggcgttt
    80161 accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa cgacgcgttg
    80221 aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg tgttccgttt
    80281 gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct tgcgcaatgt attgccgggt
    80341 atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga cgaagatttt
    80401 attatcgata ccctacccgg ccacgataat acactgctca ttaccggcct gagtgggcac
    80461 ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca agaccaaaaa
    80521 agcgattttg atttgacgcc attcaggctt tcccgcttcc aataatcatc attacggcct
    80581 ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac tatctcatca
    80641 ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg gcaattatgg
    80701 atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac aaatatttaa
    80761 tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta cagcactcac
    80821 cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta ttaattcaaa
    80881 attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca attcattctc
    80941 cgacacgcca ttttctttct ctcgcaaatt cgtattggat gcacttatgt cattcacaaa
    81001 tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg caattattgt
    81061 ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg atggctggta
    81121 tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg ggctggttat
    81181 tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt attcgcccat
    81241 gactcgtctt gccgcaaaag ctggacatct cgcattgtac ctattgctgt tcgccatcgg
    81301 catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt ttggctggtt
    81361 tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg gtgccctgca
    81421 tttttggctc gcgtggagcg tcgtagtact gtctgttatg cacggattta tggccctgaa
    81481 gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt cgtcatctga
    81541 ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt ccctgatgtt
    81601 ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc agcacgcctt
    81661 tgttaacttc cgcatacagc atcttggcta tagctggtta tatggcacct ttaaagattt
    81721 cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga atgtgacaat
    81781 taacaccacc agcgtcgata ctaaccacgc cgaacgcgat aaacatcttc gcagtgcgga
    81841 ttttctcaat accacaaaat atccacaggc aacattcacc tccaccagcg tgaagaaaga
    81901 cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca aacccgtcac
    81961 gctggaagcg aaattaattg gtcagggcga cgacccatgg ggcggtaaac gtgcaggctt
    82021 tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt taggtccagc
    82081 ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt aagagtgaaa
    82141 tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag gaatacccag
    82201 tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt cacgtcctgc
    82261 ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa tctcactaca
    82321 acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt ggcagccatc
    82381 atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat gcgcgataat
    82441 ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc caataaaacg
    82501 caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc cctcgatatg
    82561 ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac aacgaatgcc
    82621 gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct cgactgcttt
    82681 tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa agtgccccac
    82741 ttcatactca tagtggttac gcatgtcgat aaatagtgca tcgggatcgt caagcatggc
    82801 gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt gaggatcgtc
    82861 aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga aggatttccc
    82921 gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga aggcatagag
    82981 ctgcgcgcga aatgtttcaa cattgctcgc agatacgctg atttgcgcgt taatgccctc
    83041 atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat ataaagcgtc
    83101 acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg
    83161 ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac
    83221 tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa
    83281 ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa attatttgtt
    83341 atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa
    83401 aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat atgacgaatc
    83461 tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg
    83521 gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc ggttgtggat
    83581 taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg
    83641 aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg
    83701 aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg gaccgccgaa
    83761 tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac
    83821 gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg cggcagtttg
    83881 gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc
    83941 tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag
    84001 gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccagat catgattacg
    84061 gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg accacgaccg
    84121 gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta
    84181 agccagatgg caaagggtat caattgatta tgctgccgcc agagtgttct ccgccactgg
    84241 atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg
    84301 caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt
    84361 cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt accacagcaa
    84421 atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata
    84481 attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg
    84541 ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg atcgtcgcct
    84601 ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc ttacccctct
    84661 acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc ggtattgtct
    84721 tcagtataat gggttcctaa acacatcatt ttagatattg aatatgagac gggcaatcag
    84781 ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc gacaatctaa
    84841 gctggttact gactggcttg ataagaaccc tgagttttac tgggattcat ccttgagctt
    84901 taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc taggtgactt
    84961 tttaaccgct atcgagaaag ggcttgttaa agctggtgat acgctactaa tcgagagttt
    85021 agaccgtctg tcacgtcagg atattgatat tgcttctgag ctattacgcc gtatcttaag
    85081 ggctggtgta gatgtagtca cactgagcga tggagaacat tacacacgag aaagtttaaa
    85141 agacccgtta gccctgatta aaagcattct gataatgcag cgggcgcatg aggaaagttt
    85201 gaggaagtct gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca tctccgaagg
    85261 tatcaaggta tctaggcgtt gtcctgcatg gttaaggctg aatgatgacc gtcgaacatt
    85321 cacaatcatt cctgataaag tggaagttgt taaacgagca tttgacctga gattacaggg
    85381 tttaagcttt tgggctatca ccagaacttt aaacgatgaa ggtcacttgt cattgaatca
    85441 atacacaccc aaacaaaagg ggtggtctga tactgctgta aagaaactac tcagaaatcg
    85501 tgctgtgatt ggatgtttca cacctgctgg acgtgaagaa gttcagggtt actaccccgc
    85561 aataatcagt gagtcactat tctatcgagt acagcaactg aacacagggc aatatggtag
    85621 ggcttctgta tcaagtaatc cgctatcagt caacttgttc aggggtatca tcaaatgttc
    85681 tgagtgtggg gctactattg ttcttggtgg atatgcctta aaacgttttg gaatgtacag
    85741 atgccctaat cgttcagcta acaggtgtag tgcaaaggct atcagtcgta agcaaacgga
    85801 tgcaactatc ctttacatgc tcgcattatg cgaccgtttt gaaactgaaa atactgacac
    85861 tattgatagc cttaaattac agcgtgaaga tttgc
//
LOCUS       DADWTH010000002        82666 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.002, whole genome shotgun sequence.
ACCESSION   DADWTH010000002 DADWTH010000000
VERSION     DADWTH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.002"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..468)
                     /locus_tag="JNY02_000096"
     CDS             complement(<1..468)
                     /locus_tag="JNY02_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBB9431784.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRE"
     gene            complement(539..2653)
                     /gene="fusA"
                     /locus_tag="JNY02_000097"
     CDS             complement(539..2653)
                     /gene="fusA"
                     /locus_tag="JNY02_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBB9431785.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2750..3220)
                     /gene="rpsG"
                     /locus_tag="JNY02_000098"
     CDS             complement(2750..3220)
                     /gene="rpsG"
                     /locus_tag="JNY02_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBB9431786.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3317..3691)
                     /gene="rpsL"
                     /locus_tag="JNY02_000099"
     CDS             complement(3317..3691)
                     /gene="rpsL"
                     /locus_tag="JNY02_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBB9431787.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3817..4104)
                     /gene="tusB"
                     /locus_tag="JNY02_000100"
     CDS             complement(3817..4104)
                     /gene="tusB"
                     /locus_tag="JNY02_000100"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBB9431788.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4112..4471)
                     /gene="tusC"
                     /locus_tag="JNY02_000101"
     CDS             complement(4112..4471)
                     /gene="tusC"
                     /locus_tag="JNY02_000101"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBB9431789.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4471..4857)
                     /gene="tusD"
                     /locus_tag="JNY02_000102"
     CDS             complement(4471..4857)
                     /gene="tusD"
                     /locus_tag="JNY02_000102"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBB9431790.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4857..5579)
                     /locus_tag="JNY02_000103"
     CDS             complement(4857..5579)
                     /locus_tag="JNY02_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB9431791.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5746..6558)
                     /gene="fkpA"
                     /locus_tag="JNY02_000104"
     CDS             complement(5746..6558)
                     /gene="fkpA"
                     /locus_tag="JNY02_000104"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB9431792.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6779..6997
                     /locus_tag="JNY02_000105"
     CDS             6779..6997
                     /locus_tag="JNY02_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBB9431793.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7046..7636)
                     /gene="slyD"
                     /locus_tag="JNY02_000106"
     CDS             complement(7046..7636)
                     /gene="slyD"
                     /locus_tag="JNY02_000106"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB9431794.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7731..7931)
                     /locus_tag="JNY02_000107"
     CDS             complement(7731..7931)
                     /locus_tag="JNY02_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBB9431795.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7941..9746)
                     /gene="kefB"
                     /locus_tag="JNY02_000108"
     CDS             complement(7941..9746)
                     /gene="kefB"
                     /locus_tag="JNY02_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBB9431796.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9746..10297)
                     /gene="kefG"
                     /locus_tag="JNY02_000109"
     CDS             complement(9746..10297)
                     /gene="kefG"
                     /locus_tag="JNY02_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBB9431797.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10428..12341
                     /locus_tag="JNY02_000110"
     CDS             10428..12341
                     /locus_tag="JNY02_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB9431798.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRQEAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12341..13363
                     /locus_tag="JNY02_000111"
     CDS             12341..13363
                     /locus_tag="JNY02_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBB9431799.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYLLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13357..13575
                     /locus_tag="JNY02_000112"
     CDS             13357..13575
                     /locus_tag="JNY02_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBB9431800.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13629..14498
                     /locus_tag="JNY02_000113"
     CDS             13629..14498
                     /locus_tag="JNY02_000113"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBB9431801.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14553..14957)
                     /locus_tag="JNY02_000114"
     CDS             complement(14553..14957)
                     /locus_tag="JNY02_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBB9431802.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15259..15891
                     /gene="crp"
                     /locus_tag="JNY02_000115"
     CDS             15259..15891
                     /gene="crp"
                     /locus_tag="JNY02_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBB9431803.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15942..18032
                     /locus_tag="JNY02_000116"
     CDS             15942..18032
                     /locus_tag="JNY02_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB9431804.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18099..19319)
                     /gene="argD/dapC"
                     /locus_tag="JNY02_000117"
     CDS             complement(18099..19319)
                     /gene="argD/dapC"
                     /locus_tag="JNY02_000117"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBB9431805.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19405..19968)
                     /gene="pabA"
                     /locus_tag="JNY02_000118"
     CDS             complement(19405..19968)
                     /gene="pabA"
                     /locus_tag="JNY02_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBB9431806.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(20000..20602)
                     /locus_tag="JNY02_000119"
     CDS             complement(20000..20602)
                     /locus_tag="JNY02_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBB9431807.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20592..20759)
                     /locus_tag="JNY02_000120"
     CDS             complement(20592..20759)
                     /locus_tag="JNY02_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBB9431808.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20864..21436)
                     /gene="ppiA"
                     /locus_tag="JNY02_000121"
                     /gene_synonym="rotA"
     CDS             complement(20864..21436)
                     /gene="ppiA"
                     /locus_tag="JNY02_000121"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBB9431809.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21707..22888
                     /gene="tsgA"
                     /locus_tag="JNY02_000122"
     CDS             21707..22888
                     /gene="tsgA"
                     /locus_tag="JNY02_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBB9431810.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23150..25693
                     /gene="nirB"
                     /locus_tag="JNY02_000123"
     CDS             23150..25693
                     /gene="nirB"
                     /locus_tag="JNY02_000123"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBB9431811.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHSSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25690..26016
                     /gene="nirD"
                     /locus_tag="JNY02_000124"
     CDS             25690..26016
                     /gene="nirD"
                     /locus_tag="JNY02_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBB9431812.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26142..26948
                     /gene="nirC"
                     /locus_tag="JNY02_000125"
     CDS             26142..26948
                     /gene="nirC"
                     /locus_tag="JNY02_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBB9431813.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26967..28340
                     /gene="cobA"
                     /locus_tag="JNY02_000126"
     CDS             26967..28340
                     /gene="cobA"
                     /locus_tag="JNY02_000126"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBB9431814.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28587..28754
                     /locus_tag="JNY02_000127"
     CDS             28587..28754
                     /locus_tag="JNY02_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBB9431815.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29049..30386
                     /gene="frlA"
                     /locus_tag="JNY02_000128"
                     /pseudo
     CDS             29049..30386
                     /gene="frlA"
                     /locus_tag="JNY02_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30407..31429
                     /gene="frlB"
                     /locus_tag="JNY02_000129"
     CDS             30407..31429
                     /gene="frlB"
                     /locus_tag="JNY02_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBB9431816.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31479..32308
                     /gene="frlC"
                     /locus_tag="JNY02_000130"
                     /pseudo
     CDS             31479..32308
                     /gene="frlC"
                     /locus_tag="JNY02_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32305..33090
                     /gene="frlD"
                     /locus_tag="JNY02_000131"
     CDS             32305..33090
                     /gene="frlD"
                     /locus_tag="JNY02_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBB9431817.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33190..33921
                     /gene="phoB"
                     /locus_tag="JNY02_000132"
     CDS             33190..33921
                     /gene="phoB"
                     /locus_tag="JNY02_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBB9431818.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34081..35166)
                     /locus_tag="JNY02_000133"
     CDS             complement(34081..35166)
                     /locus_tag="JNY02_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBB9431819.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35178..36482)
                     /locus_tag="JNY02_000134"
     CDS             complement(35178..36482)
                     /locus_tag="JNY02_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBB9431820.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36494..36847)
                     /locus_tag="JNY02_000135"
     CDS             complement(36494..36847)
                     /locus_tag="JNY02_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBB9431821.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36858..37736)
                     /locus_tag="JNY02_000136"
     CDS             complement(36858..37736)
                     /locus_tag="JNY02_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBB9431822.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELV
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37733..38959)
                     /locus_tag="JNY02_000137"
     CDS             complement(37733..38959)
                     /locus_tag="JNY02_000137"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBB9431823.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38960..40123)
                     /locus_tag="JNY02_000138"
     CDS             complement(38960..40123)
                     /locus_tag="JNY02_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBB9431824.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40207..40569)
                     /locus_tag="JNY02_000139"
     CDS             complement(40207..40569)
                     /locus_tag="JNY02_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB9431825.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40586..41491)
                     /locus_tag="JNY02_000140"
     CDS             complement(40586..41491)
                     /locus_tag="JNY02_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431826.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41761..42765)
                     /gene="trpS"
                     /locus_tag="JNY02_000141"
     CDS             complement(41761..42765)
                     /gene="trpS"
                     /locus_tag="JNY02_000141"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBB9431827.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42758..43516)
                     /gene="gph"
                     /locus_tag="JNY02_000142"
     CDS             complement(42758..43516)
                     /gene="gph"
                     /locus_tag="JNY02_000142"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBB9431828.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43509..44186)
                     /gene="rpe"
                     /locus_tag="JNY02_000143"
     CDS             complement(43509..44186)
                     /gene="rpe"
                     /locus_tag="JNY02_000143"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBB9431829.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44204..45040)
                     /gene="dam"
                     /locus_tag="JNY02_000144"
     CDS             complement(44204..45040)
                     /gene="dam"
                     /locus_tag="JNY02_000144"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBB9431830.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45147..46433)
                     /gene="damX"
                     /locus_tag="JNY02_000145"
     CDS             complement(45147..46433)
                     /gene="damX"
                     /locus_tag="JNY02_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBB9431831.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46525..47613)
                     /gene="aroB"
                     /locus_tag="JNY02_000146"
     CDS             complement(46525..47613)
                     /gene="aroB"
                     /locus_tag="JNY02_000146"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBB9431832.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47670..48191)
                     /gene="aroK"
                     /locus_tag="JNY02_000147"
     CDS             complement(47670..48191)
                     /gene="aroK"
                     /locus_tag="JNY02_000147"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBB9431833.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48592..49830)
                     /gene="hofQ"
                     /locus_tag="JNY02_000148"
     CDS             complement(48592..49830)
                     /gene="hofQ"
                     /locus_tag="JNY02_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBB9431834.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQASSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49742..50146)
                     /gene="hofP"
                     /locus_tag="JNY02_000149"
     CDS             complement(49742..50146)
                     /gene="hofP"
                     /locus_tag="JNY02_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBB9431835.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50136..50576)
                     /gene="hofO"
                     /locus_tag="JNY02_000150"
     CDS             complement(50136..50576)
                     /gene="hofO"
                     /locus_tag="JNY02_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBB9431836.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50560..51099)
                     /gene="hofN"
                     /locus_tag="JNY02_000151"
     CDS             complement(50560..51099)
                     /gene="hofN"
                     /locus_tag="JNY02_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBB9431837.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51099..51878)
                     /gene="hofM"
                     /locus_tag="JNY02_000152"
     CDS             complement(51099..51878)
                     /gene="hofM"
                     /locus_tag="JNY02_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBB9431838.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51998..54550
                     /gene="mrcA"
                     /locus_tag="JNY02_000153"
     CDS             51998..54550
                     /gene="mrcA"
                     /locus_tag="JNY02_000153"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBB9431839.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54715..55275)
                     /gene="nudE"
                     /locus_tag="JNY02_000154"
     CDS             complement(54715..55275)
                     /gene="nudE"
                     /locus_tag="JNY02_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBB9431840.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55595..57730
                     /gene="igaA"
                     /locus_tag="JNY02_000155"
     CDS             55595..57730
                     /gene="igaA"
                     /locus_tag="JNY02_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBB9431841.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRQRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLVLNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57795..58463
                     /gene="yrfG"
                     /locus_tag="JNY02_000156"
     CDS             57795..58463
                     /gene="yrfG"
                     /locus_tag="JNY02_000156"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBB9431842.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58474..58875
                     /gene="hslR"
                     /locus_tag="JNY02_000157"
     CDS             58474..58875
                     /gene="hslR"
                     /locus_tag="JNY02_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBB9431843.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58900..59778
                     /gene="hslO"
                     /locus_tag="JNY02_000158"
     CDS             58900..59778
                     /gene="hslO"
                     /locus_tag="JNY02_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBB9431844.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59913..61637)
                     /locus_tag="JNY02_000159"
     CDS             complement(59913..61637)
                     /locus_tag="JNY02_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBB9431845.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            62016..63638
                     /gene="pckA"
                     /locus_tag="JNY02_000160"
     CDS             62016..63638
                     /gene="pckA"
                     /locus_tag="JNY02_000160"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBB9431846.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63714..65066)
                     /gene="envZ"
                     /locus_tag="JNY02_000161"
     CDS             complement(63714..65066)
                     /gene="envZ"
                     /locus_tag="JNY02_000161"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBB9431847.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65063..65782)
                     /gene="ompR"
                     /locus_tag="JNY02_000162"
     CDS             complement(65063..65782)
                     /gene="ompR"
                     /locus_tag="JNY02_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBB9431848.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66010..66486
                     /gene="greB"
                     /locus_tag="JNY02_000163"
     CDS             66010..66486
                     /gene="greB"
                     /locus_tag="JNY02_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBB9431849.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66583..68904
                     /locus_tag="JNY02_000164"
     CDS             66583..68904
                     /locus_tag="JNY02_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBB9431850.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQYPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69342..69569
                     /gene="feoA"
                     /locus_tag="JNY02_000165"
     CDS             69342..69569
                     /gene="feoA"
                     /locus_tag="JNY02_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBB9431851.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69586..71907
                     /gene="feoB"
                     /locus_tag="JNY02_000166"
     CDS             69586..71907
                     /gene="feoB"
                     /locus_tag="JNY02_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBB9431852.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71907..72143
                     /gene="feoC"
                     /locus_tag="JNY02_000167"
     CDS             71907..72143
                     /gene="feoC"
                     /locus_tag="JNY02_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBB9431853.1"
                     /translation="MASLIQVRDLLALRCRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72346..73224
                     /gene="rpnA"
                     /locus_tag="JNY02_000168"
     CDS             72346..73224
                     /gene="rpnA"
                     /locus_tag="JNY02_000168"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBB9431854.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73297..73566
                     /locus_tag="JNY02_000169"
     CDS             73297..73566
                     /locus_tag="JNY02_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBB9431855.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73560..73925
                     /locus_tag="JNY02_000170"
     CDS             73560..73925
                     /locus_tag="JNY02_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB9431856.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73926..74714)
                     /gene="bioH"
                     /locus_tag="JNY02_000171"
     CDS             complement(73926..74714)
                     /gene="bioH"
                     /locus_tag="JNY02_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBB9431857.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74752..75435
                     /gene="gntX"
                     /locus_tag="JNY02_000172"
     CDS             74752..75435
                     /gene="gntX"
                     /locus_tag="JNY02_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBB9431858.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75494..76069
                     /gene="nfuA"
                     /locus_tag="JNY02_000173"
     CDS             75494..76069
                     /gene="nfuA"
                     /locus_tag="JNY02_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBB9431859.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76430..77734
                     /gene="gntT"
                     /locus_tag="JNY02_000174"
     CDS             76430..77734
                     /gene="gntT"
                     /locus_tag="JNY02_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBB9431860.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77779..79863)
                     /gene="malQ"
                     /locus_tag="JNY02_000175"
     CDS             complement(77779..79863)
                     /gene="malQ"
                     /locus_tag="JNY02_000175"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBB9431861.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79873..82266)
                     /gene="malP"
                     /locus_tag="JNY02_000176"
     CDS             complement(79873..82266)
                     /gene="malP"
                     /locus_tag="JNY02_000176"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBB9431862.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19817 a        22455 c        20858 g        19536 t
ORIGIN      
        1 ttcacgaact tccatttcaa ccagttccag cagctcttcg tcatcaacca tgtcgcattt
       61 gttcaggaac acgatgatgt acggaacgcc tacctgacga cccagcagga tgtgctcacg
      121 agtctgcggc atcgggccgt cagtcgcagc aactaccagg atcgcgccgt ccatctgagc
      181 agcaccggtg atcatgtttt taacatagtc ggcgtgcccc gggcagtcta cgtgtgcgta
      241 gtgacgggtc ggggtgtcat attcaacgtg agaagtgttg atggtgatac cacgagcttt
      301 ttcttccggc gcgttatcga tctggtcgaa tgcacgagca gcaccgccgt aggttttagc
      361 cagtacggtg gtgattgcag cggtcagagt agttttaccg tggtcaacgt ggccgatagt
      421 accaacgtta acgtgcggtt ttgtacgttc aaatttttct ttagacacgg ctatattcct
      481 tactatagtg ctctcccctt caggagagag cacgggactt tggtattaac ccttaggctt
      541 atttaccacg ggcttcaatt acggcctgag caacgttact cggcgcttca tcatacttca
      601 ggaattccat agtgtatgat gcacgacctt tggtcagaga acgcagctga gttgcgtatc
      661 cgaacatttc agacagcggt acttcagcgt ggatcttaac gccagtaact tcagattcct
      721 gacctttgag cataccacga cgacggctca agtcaccgat aacgtcaccg gtgttctctt
      781 ccggagtttc tacttcaacc ttcatgatcg gctcaagcag aactggtttc gctttcttaa
      841 agccttcttt aaaggcgata gaagcagcca gtttaaacgc cagttcagag gagtcaacgt
      901 catggtaaga accgaagtgc agacgaatac ccatgtctac taccgggtag cctgccagcg
      961 gacctgcttt cagctgttcc tggatacctt tatcaacggc cgggatgtat tcgccaggga
     1021 ttacaccacc tttaatgtcg ttgatgaact cgtagccttt cgggtttgaa cccggctcca
     1081 gcgggtacat gtcgataaca acatgaccat actgaccacg accaccagac tgtttcgcgt
     1141 gtttaccttc aacatcggta actttctggc ggatagtttc acggtaagca acctgcggtt
     1201 tacctacgtt cgcttcaacg ttgaattcac gcttcatacg gtcaacgatg atgtcgaggt
     1261 gcagttcgcc catacccgcg atgatggtct ggttagattc ttcgtcagtc catacacgga
     1321 aagacgggtc ttctttagcc agacggccca gagccagacc cattttttcc tggtcagctt
     1381 tggttttcgg ttcaactgcg atggagatta ccggctcagg gaattccata cgttccagaa
     1441 tgatcggcgc atccgggtca cacagggtgt caccagtggt tacgtctttc agaccgatag
     1501 cagcagcgat gtcgcccgcg cgaacttctt tgatctcttc acgtttgtta gcgtgcatct
     1561 gaacgatacg accgaaacgc tcacgtgcag ctttcacgga gttcagtacg gtatcaccag
     1621 agttaaccac accggagtaa acacggaaga aggtcaggtt accaacaaac gggtcggtag
     1681 cgattttgaa cgccagtgca gagaacggct cgtcatcact tgcgtgacgt tcagccggag
     1741 tgtctttacc gtcgtccagg ataccgttga tcgcaggtac gtcaaccggg gatggcaggt
     1801 aatcaattac cgcatccagc atcgcctgaa cacctttgtt cttgaacgca gaaccacagg
     1861 ttaccaggat gatttcgttg ttcagaacgc gctgacgcag agcacctttg atttctgctt
     1921 cagtcagttc ttcaccaccc aggtattttt ccatcagctc ttcagaagct tcagctgcgg
     1981 attcgatcag gttctggtgc cattcgttag ccagttcaac catgtctgcc gggatatctt
     2041 cgtattcgaa ggttacgccc tggtcagcgt cgttccagtt gatagctttc attttcacca
     2101 ggtcaacaac accggtgaaa tgttcttcag caccaatcgc cagctgcagc ggaaccgggt
     2161 tcgcgcccag acgggttttg atctggttaa caactttcag gaagttcgca cccatgcggt
     2221 ccattttgtt aacgaacgca atgcgcggaa ctttatattt gtttgcctga cgccatacgg
     2281 tttcagactg cggctgaaca ccaccaactg cgcagtaaac cattaccgca ccatcgagaa
     2341 cacgcatgga acgttctact tcgattgtga agtcaacgtg ccccggggtg tcgatgatgt
     2401 tgatgcgatg cggctcatac tgcttagcca taccagacca gaatgcagta gtcgcagcgg
     2461 aagtgatggt aataccacgt tcctgctcct gctccatcca gtccatggtt gcagcgccgt
     2521 catgaacttc accgatttta tggtttacac cggtgtagaa cagaatacgt tcggtagtag
     2581 tggttttacc ggcgtcgatg tgcgcactga taccgatgtt acggtagcgt gcgatgggtg
     2641 ttgtacgagc catttgtttc ctcgtttatc ttttaggcgt tcaatttaag tagcccaaag
     2701 cgggctgctt actggaagcg cccgcctggt gactaaaact ccgaagggat taccaacggt
     2761 agtgtgcgaa cgccttgttg gcttcggcca tacggtgaac gtcttcacgt ttcttaactg
     2821 cagtaccttt gttttctgca gcatcagaaa gttcgttcgc caggcgcaga gccatggatt
     2881 tatcaccgcg tttacgagca gcttcaacga tccaacgcat tgccagagca ttacgacgaa
     2941 ccggacggac ttcaactggt acctgataag tagaaccacc aacgcggcga gacttaactt
     3001 ctacagtcgg gcgcacgttt tcgagagcta cttcgaatgc ttccagttca gatttaccag
     3061 agcgctgagc cagggtctcc agcgcgctgt atacgataga ttcagcagta gattttttac
     3121 catctaccat caggatattt acaaatttag ccagcagttc tgatccgaac ttcggatccg
     3181 gcagaatttt acgctgacca atgacgcgac gacgtggcat ggaaatactc cgttgttaat
     3241 tcaggattgt ccaaaactct acgagtttag tttgacattt aagttaaaac gtttggcctt
     3301 acttaacgga gaaccattaa gccttagggc gcttcacgcc atacttggaa cgagcctgct
     3361 tacggtcttt aacgccggag cagtcaagcg caccacgtac ggtgtggtaa cgaacacccg
     3421 ggaggtcttt aacacgaccg ccacggatca ggatcacgga gtgctcctgc aggttgtgac
     3481 cttcaccacc gatgtaggaa gtcacttcga aaccgttagt cagacgaaca cggcatactt
     3541 tacgcagcgc ggagttcggt tttttaggag tggtagtata tacacgagta catacgccac
     3601 gtttttgcgg gcatgcttcc agcgcaggca cgttgctttt cgcaactttg cgagcacgtg
     3661 gtttgcgtac cagctggtta actgttgcca ttaaatagct cctggtttta gcttttgctt
     3721 cgtaaacacg taataaaacg tcctcacaca atatgaggac gccgaatttt agggcgatgc
     3781 cgaaaaggtg tcaagaaata tacaacgatc ccgccatcac caggccatct ggctggagtg
     3841 cttaaccgta agtctgacga aatcagtata gtcaatggga atgatgtcgt tcaaaatttg
     3901 accagtcaaa ccgcgggcaa taaggtcttc gttcagggca tagaccttaa tgggggcatt
     3961 acgcagactt tcaaggtagc ggttaccgtc aactgcggca gttacgccat cttgcaataa
     4021 tagcagttcg tctccttcac tgagcagacg cagcagcgca gcaaaatccg tcagccaggg
     4081 tgaacgatgt aacgtgtgca gcataaacgc ctcaaaacct caaaataaca tcgtagttgg
     4141 cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgacaaca aagggtgtct
     4201 gcggatctaa cccgcgttcg cgcagtgaag ccgcacaaac ccagcactgt tcaatgtcgt
     4261 acagactcaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca tcgggctttt
     4321 gtcctgacag cagctgaaaa acgccatcag caataaagaa gacggccaga tcgtcagtta
     4381 atgcggaagt tgccagtaaa gcatctaaac cttcccgacc agcagctgta ccatgaggtg
     4441 cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt cacaggtcag
     4501 cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccggctgaa ggtttgacga
     4561 agccagcccc agccttcctg cttcagtttc atcgacgata ccgcggcgta acgctgccgc
     4621 tacgcagata ttcagcgcca caccatgttg cgcattcaac tgttgccagc cgcgcacaag
     4681 gtcaaattcg tcgcttgccg gtgaggtcag ttggttagcg ttatagaccc cttcccgata
     4741 gaaaaagacg ctgcttaact tatggccttc tgctatcagc gcctgcgcaa actgaaaagc
     4801 actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa aacgcattac
     4861 ttatcttgcc cctgaaaatc accgctcttg aactggcgga tgtagagata gacagtgtgt
     4921 ttggagatgt tcaggcggtc agcaacctgg ttgatcgcat ctttaatatc gaagatccct
     4981 ttctcgtaga gattcagcac gatctgacga tttttggcgt tattagaaac attgcgatcg
     5041 gcattcactt cttcgatggt gaactccagc gtttgggtaa ccagatcttc aacagaagag
     5101 gcaaagttga cgcttgaacc gacatccggg gtttctggcg gcacaaaagt gctcataatc
     5161 tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac acgctgttcg
     5221 cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc gcgagtaaag
     5281 tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg tagcgccagg
     5341 tcagtaattg gcgaaccaat cttccggcct gtatgttcac cgttagcaat gcgaatggct
     5401 gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc aataagcatc
     5461 gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt ctggtcgaaa
     5521 ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga cctggacatg
     5581 aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc tcattaatga
     5641 tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt atatccggcc
     5701 tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt aatgcttatt ttttagcaga
     5761 atctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg gtttcacatc
     5821 cagcagctct acgtcaaaca ctagggtaga attcggtggg atccccggaa cacccgcttt
     5881 gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct tgatgttctt
     5941 cagaccttct gtccaacccg ggataacacc gtccagacgg aaagaaagcg gttcaccacg
     6001 ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt tcactacaac
     6061 agtatcgctg tctttcggtg cttcgccttt accggcttct actacctgat aaaccagacc
     6121 agttgaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact ctttaccttt
     6181 tgcttcgtta tcagccgcgt ctttttccat cttcgcctga gcagaagact tcacgcgagc
     6241 ttcgaatgct tgcagagtct gttcgatctc ttggtcggag agtttgctct tatcagcaaa
     6301 tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca gtttttcttg
     6361 ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat aagctgattt
     6421 ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt ttgcagcttc
     6481 agcagcaaaa gtgattggtg catgcagggc aacggccatt gtggtcgcca gtagcgttac
     6541 tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca gggttaacta
     6601 ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca cgccctatta
     6661 tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa atgagtgctg
     6721 tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga ggtgaatcat
     6781 gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg cttttcagga
     6841 aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga tggcgaaact
     6901 gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc agccagccgt cgaacatcgc
     6961 gtcgcaggct gaagaaacgc caccgccaca ttattgaggc gtaaaaaaag cggggattcc
     7021 ccgctttttt gtcacctttt cggtattagt ggcaaccaca accgccgttg cctttaccgc
     7081 cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgaccg ccgcagcaac
     7141 cgtcgtggtc gtgatcgtgg tgatgatcgt gcgcgccgtg aacgtggcca tgagccagtt
     7201 cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg ttctgaccgg
     7261 ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg atttcaaccg
     7321 gtaccggacc ctggtcagtt tcagccagga aacgcatacc tacctgcagt tcatcaacgc
     7381 ccataaatac gtctttagga acacgttgca ctaggttttc gtcgtactga ccgtaagcgt
     7441 cgttcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc agcgccgttt
     7501 ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca ctcaccggag
     7561 actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg accaccaggt
     7621 cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca gattgtagcg
     7681 gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg ctagtccgga
     7741 tgaaaaatcc cgatcacttg ctcatctttg tgaacgtgat cgcgggcttc tttgtctgct
     7801 tctcgcatct gatgtccgca cttaacacat tcaacaatat caatattatt ttcgcgccac
     7861 atcgccattg aatcctgcgc ctgacaggcc gggcattttg cgcccgctat aaaacgtttt
     7921 cggattgcca tccttaccct ctactcaaat tcatcccagc cgtccagctg gcgtcgttct
     7981 tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct ggcgcgagaa
     8041 atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat atccaggcgg
     8101 cgaaaatgca gttgcgcccg ctgtgcctga tgcggatgca tgccaagcgt gaccagcgtc
     8161 ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt cacccctgcc
     8221 tgtaataact catgcgcttc cacacgtccg cgcactcgcg caagaatatg caaatgcgga
     8281 aagtgctgct ggcatatttc caccagcttc atggtgtctt ccggctcgtt acaggtaatg
     8341 acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatcgacctg cgtggcgtcg
     8401 ccgtaataaa ctttgtagcc atatttacgc atcaggttaa cggcgctgat atcccgctcc
     8461 agcacggtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg accaaaacgc
     8521 ccgaagccca cgacaatgac ctggggttta tcatcgttga cccacggttt ttcgtcttct
     8581 tcttccggtc cgttaaactg gcgggatagc catttatcca ccagcttcat cagcaacggc
     8641 gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc gccctggaat
     8701 aaacgttgtg aagaagcggt agaaaagagg acaaaggcaa actcaccgcc ctgactcaac
     8761 acgccagcaa actgcatccg ctctgagcta cgcacgccat acaatcgcgc cagcagatac
     8821 agcacgagaa ttttcaccgc caccagcaca accacgctta tcactaccca caacagatgg
     8881 gtataaagca ccccgaggtt gagcgacatg ccgacagaga taaagaacaa accgagcagc
     8941 aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact ttccgccagc
     9001 agcacgcccg caataaatgt accgagcgcc atcgacagcc ccagcgcatc cataaacaat
     9061 gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac gccagaagct
     9121 gcaataaagc ggaataccgg acgcagtaaa tagcgcccac caatcaacat gccgacaaac
     9181 gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga ccccgccaac
     9241 aacggcacta acgccagtgc tgggatcact gccagatcct gaaacagcag aaccgaaaac
     9301 ccgagctggc cggattcgct gcgattcatc cctttctcac gcatcaattg caacgccatt
     9361 gcagttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca ggcgaaatcc
     9421 gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca gcacctgtgc cacgcctacg
     9481 ccaaaaatcg aacgccgcag ttgccaaagt ttggagggat tcaactcaag gccgatgata
     9541 aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac gtcgctaata
     9601 aaccccagcc cccacgggcc aattgcaatc cctgccagca aatatcccaa cacagcgcca
     9661 atccccagcc gcgatgccaa cggcaccgca gccaccgccg cgaagagaaa cagcactcct
     9721 gcgagtaaaa aatcggaacc ttccatcagc ggcctcctgg agacagcgga tttgccaacc
     9781 agtcaccgta ggctctggcg tggctcgcca gctcctttgc gctttgccgt ctcgcccagt
     9841 aaataatgat ggaacttaac caatgcatcc ggcacatgcc cgccgccagt tcaaaggggc
     9901 gcagcacatc gctcatcggg tagcgattca gcgcgtcata acggtaagca ctttccggct
     9961 cgccggtggt aatcacgtta cgccagtact ttcccgccag ttggtttcct cccggcccgc
    10021 tggcaaaacc acgacttaat acccggtcca gccactcttt cagtagcgcc gggcagctat
    10081 aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat gcctgctcac
    10141 gggggatatc aataaaaaaa tcgggatagt gcgcgtaaag gtcgtgcacg gtaacattgc
    10201 tgagctgcgt ggccggttta agcagtaccc ggtttgccac cgagtcctga gattccggat
    10261 gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg gtatggtttt
    10321 tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg cccatacatt
    10381 acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg attgttttct
    10441 cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc gccaccatca
    10501 accccgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct accctgctgg
    10561 cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg ggaagctggc
    10621 aactggcgtg ggtgaatcag gaaacgccag cgttaccgca agcggcgctg gaatatgtca
    10681 ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct gcacgacgcc aacgaacgta
    10741 acgacgggca cgccattgcg accattcata gcaagctgga tgctattgac gcatggagta
    10801 ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa caactggagc
    10861 gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc caggcgctga
    10921 tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat ctcgatgccg
    10981 ttatctggct ggaaaaatgg ttgaagagct atcagggcac gctgatcctg atctctcacg
    11041 accgcgactt cctcgatccg atcgttgata aaattattca gatcgaacaa caaagcatgt
    11101 tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt ctggcgcagc
    11161 aacaagcgat gtacgaaagc cagcaggaac gcgtggcaca tctgcaaagt tatatcgatc
    11221 gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgcattaag atgctggaac
    11281 gtatggagct gattgccccc gcacacgtcg acaacccgtt ccgctttagc ttccgcgcgc
    11341 cggaaagcct gccaaatccg ttactgaaga tggaaaaggt cagcgcaggc tatggcgatc
    11401 gcattattct cgactcgatt aaattgaatc tggtccccgg ctcgcgcatt ggtctgttag
    11461 gccgcaacgg cgcaggtaaa tcgacattaa tcaaactgtt agccggtgaa cttgcgccag
    11521 tcagcggtga aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc cagcatcaac
    11581 ttgaatacct gcgcgccgac gaatcaccta ttcaacatct ggcacgttta gcgccgcagg
    11641 agctggaaca aaaactgcgt gactacctcg gcggctttgg tttcctgggc gataaagtaa
    11701 ccgaagaaac gcgccgcttc tccggtgggg aaaaggcccg cctggtgctg gcattaattg
    11761 tctggcagcg tccgaatctg ctgctgctcg acgaaccgac taaccacctt gacctcgaca
    11821 tgcgtcaggc agtcaccgaa gcattaatcg agtttgaagg cgcgctggtt gtcgtttcgc
    11881 acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac gatcgtaaag
    11941 tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac gtacaaaagc
    12001 aggaaaacca gaccgacgaa gcgccaaaag agaacgcgaa cagcgcccag gcacgtaaag
    12061 atcagaagcg tcaggaagcg gagctgcgtg cgcaaaccca gccgctgcgt aaagagattg
    12121 cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg gaagagaaac
    12181 tcggcgacag cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc tgcctgcaac
    12241 agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg gaagcccagg
    12301 agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga taacgacgac
    12361 cgatgccaat gaatttagca gcagtgctga attcacccct atgcgcggct ttagcaattg
    12421 tcatctacaa actatgctgc cgcgtttgtt tcgtcgtcag gtgaaattca ccccgtactg
    12481 gcagcggctg gagttgcccg acggcgattt tgtcgatctc gcgtggagtg aagaccccgc
    12541 acaggcgaaa cataaaccgc gtttagtggt gtttcacggg ctggaaggca gtctcaatag
    12601 cccttacgcc cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg gcgtggtgat
    12661 gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc attcgggcga
    12721 aaccgaagac gccagttggt ttttacgctg gctgcaacgc gaatttggtc atgcgccaac
    12781 ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg caaaggaagg
    12841 caatgatctc ccggttgatg cggcggtgat tgtctctgcg ccgtttatgc tggaagcctg
    12901 tagctatcat atggaaaagg gcttttcccg cgtttatcag cgttacttgc tgaacctgtt
    12961 aaaagccaat gccgcgcgca agctggcagc ctaccccgga acgctgccga ttaatctcgc
    13021 gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctaatcaccg ccagaattca
    13081 cggctacgcc gacgctatcg actattatcg tcagtgtagc gccatgccga tgctgaaccg
    13141 gatcgccaaa ccgacgctga ttattcacgc caaagacgat ccgtttatgg atcatcaggt
    13201 gatcccaaaa ccggaaagtc tcccctcgca ggtggagtat ctactgactg aacatggcgg
    13261 tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg agtcacgcat
    13321 tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg gcaagacctc
    13381 tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg caccgattat
    13441 ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg tcaaacgcca gctacagtgc
    13501 ggagaagcgg tgctggtatg gtcggaactg cacgaaacgg tcaatattat gccgcgcagt
    13561 cagtttcgcg aataacctgc aaccgtcggc ggacgagata atagtgatag ctaagtcatg
    13621 gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc ggcgcgggga
    13681 ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg catgcagctg
    13741 aggtggaagg cgacagtttt caccgttaca cccgcccgga aatggacatg gcgatccgca
    13801 aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac gacttcggcc
    13861 tgctggaaca aaccttcatt gaatacggtc agagcggcaa agggaaatct cgcaaatatc
    13921 tgcataccta cgacgaagcc gtaccctgga atcaggtacc gggaacattc accccctggc
    13981 aacctttacc agaacccact gatgtactgt tttatgaagg tttacacggc ggcgtagtca
    14041 cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtagtg cctatcgtta
    14101 accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac tcacgagaag
    14161 cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc acaccgcagt
    14221 tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacttca aacccgttcg
    14281 cggcaaaagg tatcccgtcg ctggatgaaa gctttgtggt gatccatttt cgtaatctgg
    14341 aagggatcga tttcccttgg ctgctggcga tgttgcaagg ctcattcatt tcccacatca
    14401 atacgttagt ggtacctggc ggcaaaatgg ggctggcaat ggaattaatt atgctgctgc
    14461 tggtgcaacg attgatggaa ggaaagaaaa tcgagtaact ttgctattat gccggataaa
    14521 atactatccg gcttcacaac gggatagtta agtcacgcgg caaccacttc atacgagtga
    14581 gtaatattca ccgctttttc cagcatcaac gccactgaac aatatttctc ggcagagaga
    14641 tcaaccgcac gcgcaaccgc tgcgtctttc aggtcgcgac cggtgacgat aaaatgcaga
    14701 ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgtt cagaggtcaa ttttacttca
    14761 caatcgacca catcctgacg ccctttttgc aggatcgaaa ccacatcgat ggcactgcaa
    14821 ccacccgccg ccatcaacac catttccatc ggacttggtg ctttatcgcc tgagttgccg
    14881 tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc ttcgacccac
    14941 ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca gagtacgcgt
    15001 actaaccaaa tcgcgcaacg gaaggcgacc taggtcatgc tgaagcgaga caccaggaga
    15061 cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatacatt gatgtactgc
    15121 atgtatgcaa aggacgtcac attaccgtgc agtacagttg atagcccctt cccaggtagc
    15181 gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga gaaagcttat
    15241 aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac tctcgaatgg
    15301 ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat tcaccagggt
    15361 gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct gatcaaagac
    15421 gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat tggcgaactg
    15481 ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac cgcctgtgaa
    15541 gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc ggacattctg
    15601 atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa agtgggcaac
    15661 ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaacct ggcaaaacaa
    15721 ccagatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca ggaaatcggt
    15781 cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct ggaagatcag
    15841 aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta atcccgtcgg
    15901 agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc agactgattt
    15961 atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg cccgtggccg
    16021 ttgggttaat gcttggcgaa ttacgattcg gtctgctctt ctccctcgtt cctgcctgtt
    16081 gcaatattgc gggccttgat acgcctcata aacgtttttt caaacactta atcattggtg
    16141 cgtcgctgtt tgccacctgt agcttactga cacagctgct actggtaaaa gatgttcccc
    16201 tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgag ctggggccat
    16261 tgcacgcaaa attgcttcct gcatcgctgc tcgccgccat ttttaccctc agtctggcgg
    16321 gatacatgcc ggtctgggaa ccattgctca tctatgcgtt gggcactctc tggtacggat
    16381 tgtttaactg gttttggttc tggatctggc gcgaacaacc gctgcgcgag tcactaagtc
    16441 tgctgtaccg tgaactggca gattattgtg aagccaaata cagcctgctt acccagcaca
    16501 ccgatcctga aaaagcgctg ccaccgctgc tggtgcgcca gcaaaaagcg gtcgatttaa
    16561 ttactcagtg ctatcagcaa atgcatatgc tttccgcgca aaacaatacc gattacaagc
    16621 ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg gtcagtttgc
    16681 atcagccgga agaggtgcaa aaactggtcg agcgtagcca tgcggaagaa gttatccgct
    16741 ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt ctttaccatc
    16801 gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa atcgcccgcc
    16861 agcatccgga taatccggtt gggcaattct gctactggca tttcagccgc atcgcccgcg
    16921 tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa cagcggcgaa
    16981 tgccgttgct tccggcactg aaaagttatc tgtcactaaa gtctccggcg ctacgcaatg
    17041 ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgtg ctgcatctgc
    17101 cgaagtcgta ctggatcctg atgacggtat tgctggtgac acaaaatggc tatggcgcaa
    17161 cccgtctgag gattgtgaat cgctccgtgg gaaccgtggt cgggttaatc attgcgggcg
    17221 tagcgctgca ctttaaaatt cccgaaggtt acaccctgac gttgatgctg attaccaccc
    17281 tcgccagcta cctgatattg cgcaaaaact acggctgggc aacggtcggt tttactatta
    17341 ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc cttccacgtc
    17401 ttatcgatac cattattggt tgtttgattg ctttcggtgg taccgtctgg ctgtggccgc
    17461 agtggcagag cgggttattg cgtaaaaacg cccatgatgc tttagaagcc tatcaggaag
    17521 cgattcgctt gattctgagc gaggacccgc aaccaacgcc actggcctgg cagagaatgc
    17581 gggtaaatca ggcgcataac actctgtata actcattgaa tcaggcgatg caggaaccgg
    17641 cattcaacag ccattatctg gcagatatga aactgtgggt aacgcacagc cagtttattg
    17701 ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg ccacctgaac
    17761 tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag cagcgactgg
    17821 agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg gagatgcagc
    17881 cgcacgaagg cgcggcaggt acgctggagc agcatttaca gcgggttatt ggccatctga
    17941 acaccatgca caccatttcg tcgatggcat ggcgtcagcg accgcatcac gggatttggc
    18001 tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct aaaccatcgg ataaggcatt
    18061 cacgccgcat ccgacatatt ttgcctgatg cgtcttattt acgccccaac caccttcgcc
    18121 accgcgtggg cgaaacgttg catcccctca tcgatatccg catcttccac caccagcgac
    18181 ggtgctaaac gcatcacatc cggtccggca ttcagcacca ttacgccaac ctctgcaccc
    18241 gcatacagga aatcacgcgc ccgacctttg tagtgtggtt tcagctccgc gccaattaac
    18301 agccccatac cgcgaatatc gctaaacaca tcgtactgct gatcgatctt ctgcaaatgg
    18361 tcaacaaaac gctggcgttt cgcctgaatg ccttccagca cttcaggggt attgatgata
    18421 tcaaacgccg cgcccgctac tgcacaggcc agaggattac cgccgtaggt ggaaccgtga
    18481 gaaccaggat gaaacgcaga agcaatttcc gccgtggtca gcatggcgct aatcgggaag
    18541 ccgccgccta acgctttcgc agaagtcaga atatccggcg tcacaccgta gtacctgtaa
    18601 gcaaacaaat cgccagtccg ccccatcccg cactgcactt catcaaacac caataatacc
    18661 tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact ctggcgtcgc tgccgtcaca
    18721 ccgccctcgc cctggatcgg ctcaaccacc accgcacagg tgtgatcatc catcaccgct
    18781 ttcactgcat ggagatcgtt aaagggaacg tggatgatgt ctgccggttg cggcccaaag
    18841 ccgtcggaat attttggctg cccacccacc gaaacggtaa acagcgagcg accatgaaaa
    18901 gcgttatgga aggcaataat tttggttttg aacgggctat gacgcacaca ggcgtaatgg
    18961 cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg agttcataaa caccacgcgt
    19021 tcggcaaacg tcgcctcaat cagtttgcgc ccaagacgca gcgccggttc attggtgaaa
    19081 acgttactga tatgccacag agtttcgccc tgggttttta acgcgttcac cagcgcagga
    19141 tggcaatggc ccaacgccgt aactgcaatg ccacccgcga aatcgacata ctccttgcct
    19201 tgctgatccc agattcggct gccctgacct tttaccggaa taaactctgc cggagcataa
    19261 atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg ctgtttgttc aattgccatc
    19321 tcatgatcac cctgttacgc ataaataaat atgaaattat aatcacaaaa tatgcataaa
    19381 aaatcactga atggcaatcg gaaatcagag atgcaggaaa ttagccagca gttgatgtcc
    19441 ttgttcgcta agaatacttt ctggatggaa ctgcacacct tccagatccc actggcgatg
    19501 gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc acgtcaaagc acgctggtaa
    19561 tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg gtaagtggat ttgccagccc
    19621 ccgaaatacg ccctcaccgc tatgtgtaat cggcgaggtt ttgccgtgca tgacctttgc
    19681 ggcgcgcaca actttgccgc caaatgcctg cgccattgcc tgatgaccga ggcagacgcc
    19741 aagaatcggc aagcgcccgg catagtggcg aataacgtca agggagatcc cggcttcatc
    19801 tggcgtacag gggccaggtg agatgacaat tttttgtggt ttaagggcgt cgatatccgc
    19861 cagcgtcaac gcatcgttgc gcttaaccag cacatccgcc cccagttcac aaaagtactg
    19921 gtagaggttc caggtaaaag aatcgtagtt atctataagc aggatcatgg cggctccggt
    19981 acgaaagaac cgcgctattt tactcagatt ccccggcttc gcttaccact ttgctaaata
    20041 tcatctgcag tgcggtgaga tcccccattg ccccactctg atttgcctga ttccaggcct
    20101 ctttttcgat accctgccag ctcagttgat atccggcatg aatcgccagt tgctcgaaga
    20161 agatccgctg tgccagacca cttcccaccc ggaagggatg cagcacgtcg atttcacaat
    20221 agtaatgcgc cagccgctcg acgaacttcg ctttctccag gccaaccaga taaccttctt
    20281 cctccagatc ctgcatcagg gcattgccct ctttttcgat ataagcaaag tggcagaacg
    20341 gcgtatcgcc ctgataaata tcaacttcac gcagttgccc tgcccagtcg aaaatatcct
    20401 ggtacagctg gcgatggata gtgcgcaaat gcggtaaacc gcgcaccagc ggaccaagct
    20461 caatggtcgc agcacgcagc gccgtcattt cgtaagcggc ctgttccagc cgctgctgct
    20521 ggcggatgtt cagccggtta cgcatgatat caaggccagg ataaagatac ggatcgcgcc
    20581 cttcgccgaa tttatcgctc atagtgcctc ctcagctctt caaggcgcgc taaagcctct
    20641 gcggcagtaa gagtgactaa aggcatctcg acgccttcaa gacggcgact ggcctggaaa
    20701 ttacgattac gctgaagctc ccagagacgg gacttttgct tatcggtgag tttcttcact
    20761 tgatgcctcc ctgaacgtgt ccatttgcca caagtataag cggcaaatcc gggttacgcc
    20821 gggagagcag aagcgcgggc aagattgccc gcgcgagaaa tcattacggc aggactttag
    20881 cggaaaggat aactaccggt tttgacggca cattctggta cggaccaaca tcatgagtcg
    20941 gcacctggga aatcttatcg gcaacgtcca tgcctttcac cactttacca aataccgcgt
    21001 aaccgaaatc acgctgacca tggtcaagga aggcgttatc ggcaacgttg ataaagaact
    21061 ggctggtggc gctgtctttg tcagcggtac gcgccatcgc gatggtgcca cgcgtgttgc
    21121 gcaggccgtt atcggcttca tttttgattg gcgggtttgg ttttttctgc tgcatctgct
    21181 cggtgaaacc gccgccctga atcataaagc caggaatgac gcggtgaaag gtagtgttgt
    21241 tataaaaacc gctgttcaca taatcgacaa agttttgcac agacactggc gctttttgtt
    21301 tatccagctc cagttcgatg ttaccagctg aggttgtcaa caatacgtgc gggtcccctt
    21361 tcgctgccat tgctgcggga gaaagagcag aaagagcgaa aacagcagcc atcgccgcca
    21421 gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc aagtgaagag
    21481 attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt atctgaaata
    21541 tatgtaacct gacgatcact ttttattccg tgatcaaaat cacctcttaa aatgcaattt
    21601 agcaaccgat tgcaataaaa catttaaaca gatcacaaaa tcacctaaaa tcgcccgctc
    21661 gcggcatatc ccgctatgcc attttatttt tgaacgcagg ccaatcatga ctaacagcaa
    21721 tcgcatcaag ctcacatgga ttagctttct ctcctacgca ctgactggtg cgttggttat
    21781 tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg tttccagtat
    21841 gagtaacacc ttcaccttcc tcaacgccgg cattttaatc tctatcttcc tcaacgcctg
    21901 gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc tgatggtgct
    21961 ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcgacgg cgatgttcat
    22021 tctcggggtg gtcagcggca ttactatgtc gattggtaca ttcctgataa cacaaatgta
    22081 tgaagggcgt cagcgcggtt cccgcctgtt atttactgac tccttcttca gtatggctgg
    22141 gatgattttc ccaatgatcg ccgcgtttct actggcgcgc agcattgagt ggtactgggt
    22201 ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttca gctgtgagtt
    22261 cccggcactg ggcaaacgtg caccaaaaac ggatgctccg gtagagaaag aaaagtgggg
    22321 gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc agttaggttt
    22381 tatctcctgg gtgcctgagt atgccaaagg cctgggcatg agcctgaacg acgcgggcac
    22441 gctggtgagt aacttctgga tgtcatacat ggtcggcatg tgggcgttca gctttattct
    22501 tcgcttcttt gatttgcaac gcattctgac cgtactggct ggtctggctg cgattctgat
    22561 gtacgtcttt aacaccggaa caccagcaca tatggcgtgg tcaattctcg ctctgggctt
    22621 cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga ccaaagtacc
    22681 gtcgccaaaa ctggttaact ttgtcctgac ctgcggaacc atcggtacta tgttgacctt
    22741 tgtggttacc ggcccgattg ttgaacatag cggtccgcag gcggcactgc ttacagcaaa
    22801 cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca gccgtcaccg
    22861 tcagcataac accctgacct ctcattaatt gctaatgccg gacggcacta tcgtcgtccg
    22921 gccttttcct ctcttccccc gctacgtgca tctatttcta taaacccgtt cattttgtcc
    22981 attttttgca caaacatgaa atgtcagaca attccgtgac ttaagaaaat ttatacaaat
    23041 cagcaacata ccccttaagg agtatataaa ggtgaatttg atttacatca ataagcgggg
    23101 ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa tgagcaaagt
    23161 cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag atcttcttga
    23221 taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc gcatcgctta
    23281 tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc tgtcgctggt
    23341 gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac gcgctatcac
    23401 catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt tttatgacaa
    23461 gctgatcatg gcaaccggtt cctacccgtg gatcccacca atcaaaggtt ctgatactca
    23521 ggactgcttt gtctatcgca ccattgaaga cctcaacgcc attgaatcct gcgcccgtcg
    23581 cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg ctggcgcgct
    23641 gaaaaactta ggtattgaaa cccacgttat cgaatttgcc cctatgctga tggcggaaca
    23701 gcttgatcag atgggtggcg agcagctgcg tcgcaaaatc gaaagtatgg gcgtgcgcgt
    23761 tcactccagc aaaaacaccc ttgagattgt gcaggaaggt gtcgaagcgc gtaaaaccat
    23821 gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta ccggtatccg
    23881 tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg gggggattgt
    23941 cattaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg aatgcgcaag
    24001 ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc aggtcgccgt
    24061 tgaccatatt ctgggtagcg aaaacgcctt tgaaggtgct gaccttagcg ccaagctgaa
    24121 actgctgggc gtagacgtag gtggtattgg tgatgcgcac ggtcgcacgc ctggcgcacg
    24181 tagctatgtt tacctcgacg aaagtaaaga gatctacaaa cgcctgattg tcagcgaaga
    24241 caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg gcaacctgct
    24301 gcaactggtg ctgaacgcta tcgagctgcc ggaaaacccg gattctctga ttctgccggc
    24361 gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca gcgcgcaaat
    24421 ctgctcctgc ttcgacgtca ctaaaggtga tctgattgct gccatcaaca aaggctgcca
    24481 cacagttgcg gcgctgaaag ctgaaaccaa agcgggtact ggctgcggtg gctgtatccc
    24541 gctggtcact caggtactga acgcggaact ggcgaaacag ggcatcgaag ttaacaacaa
    24601 cctgtgcgaa cactttgctt attcgcgtca ggaactgttc catttgatcc gcgttgaagg
    24661 cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggctacggtt gtgaagtttg
    24721 taaaccaacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc tgaagccgga
    24781 acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaga aagacggcac
    24841 ctactcggtg atcccgcgtt ctccgggcgg tgaaatcacc ccggaagggc tgatggcagt
    24901 gggtcgtatc gcgcgtaaat ttaatctcta caccaaaatc accggctccc agcgcctggc
    24961 gatgtttggc gcacagaaag acgatctgcc ggaaatctgg cgtcagctga ttgaagctgg
    25021 cttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct gcgtgggtag
    25081 cacctggtgc cgctacggtg ttggcgatag cgtcggcctc ggcgtggaac tggaaaaccg
    25141 ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct gtacccgtga
    25201 atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag gctggaacct
    25261 gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg cagcggatat
    25321 cgatcgcgaa acgctgatca aatatctcga ccgcttcatg atgttctaca tccgtaccgc
    25381 cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggcggca tcgattacct
    25441 gaaagcggtg atcattgacg acaaactggg gctgaacgct catctggaag aagagatggc
    25501 gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaatacac cgtccgcgca
    25561 gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc agatggtgcc
    25621 agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa ctctggtgga
    25681 ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct gcctgaaacc
    25741 ggcgtctgcg cgctgttagg tgacgagcag gtagcgattt tccgcccgta tcacagcgac
    25801 caggtgtttg cgatcagcaa cattgatccg ttcttcgagt ccagcgtgct atcacgcggg
    25861 ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa acagcgtttt
    25921 cgcttaagtg atggcttgtg catggaagac gaacagtttt ccgtcaaaca ttacgaagca
    25981 cgagtgaaag acggcgtggt gcagctgcgc ggttaatgtt ttaacgggag gcgcaatgcc
    26041 tccccttttt gcatggtcct gtaatgacct tcggtatatt gcaggacatt ttttaaactt
    26101 tttgttttat tttttgtttt tattttttaa aggataatca aatgtttaca gacactatta
    26161 ataagtgtgc ggctaatgct gcgcgcattg cacgcctgtc ggcaaataac ccgctcggct
    26221 tttgggtcag ctccgccatg gcgggcgcat atgtgggtct tgggatcatc ctgattttca
    26281 cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt gatgggcgcg acctttggta
    26341 tcgccttaac gctggtgatt atcgccggtt ctgaactgtt caccggacac accatgttcc
    26401 tcacctttgg ggtaaaagcg ggcagcatca gccacgggca aatgtgggca atcctgccgc
    26461 aaacctggct gggtaacctg gtcggttccg tcttcgttgc catgctctat agctggggcg
    26521 gcggtagcct gctgccggta gataccagca tcgttcactc cgtcgcgctg gctaaaacca
    26581 ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg taactggctg gtttgcctgg
    26641 caatctggat ggcgctgcgc actgaagggg cggcgaaatt tatcgctatc tggtggtgtc
    26701 tgctggcatt tatcgcgtcc ggctacgagc actctatcgc taacatgacg ctgttcgcgc
    26761 tctcctggtt cggcaaccac agcgaagcct acacgctggc gggtattggt cataacctgc
    26821 tgtgggtgac gctgggtaat actttatcag gtgccgtatt catgggattg ggttattggt
    26881 atgctacgcc gaaagcgaat cgtccggttg cggacaaatt taatcaaact gaaacggctg
    26941 ccggttaatt actaaggggt ttttacgtgg atcatttgcc tatattttgc caattacgcg
    27001 atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga acgcaaagca aggttgctgt
    27061 tagacgcagg cgctcgctta acggtgaatg cattagcgtt tattccacag ttcaccgcat
    27121 gggcagatgc aggcatgtta accctcgtcg aagggccatt tgatgaaagc cttctcgaca
    27181 cctgctggct ggcgattgca gcgacggatg atgacacgct taaccagcgc gtcagcgaag
    27241 ccgctgaagc tcgtcgcatc ttctgtaacg tagtcgatgc gccgaaagcc gccagcttta
    27301 ttatgccgtc gattattgac cgctcaccgc taatggtagc ggtctcctct ggcggcacct
    27361 ctccggttct ggctcgcctg ttgcgcgaaa aactcgaatc actgctgccg ttgcatctgg
    27421 gccaggtggc gaaatatgcc gggcaattac gcgggcgagt gaaacaacag ttcgccacga
    27481 tgagtgagcg tcgccgtttc tgggagaaat tgttcgttaa cgaccgcctg gcgcagtcgc
    27541 tggcaaacaa cgatcagaaa gccattactg aaacgaccga acagttaatc aacgaaccgc
    27601 tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc gggcgatgcc gggctgctga
    27661 cgctgaaagg actgcaacaa attcagcagg cagatgtggt ggtctacgac cgtctggttt
    27721 ctgacgatat tatgaatctg gtacgccgcg atgctgatcg cgttttcgtc ggcaaacgcg
    27781 cgggatacca ctgtgtaccg caggaagaga ttaaccagat cctgctgcgg gaagcgcaaa
    27841 aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt tatttttggc cgtggtggcg
    27901 aagagctgga aacactgtgc aacgcgggta ttccgttctc ggtggttccg ggtattaccg
    27961 cagcttctgg ttgctctgcc tattcgggta ttccactcac gcatcgcgat tatgcccaga
    28021 gcgtacgctt aattaccgga cacttaaaaa ccggtggcga gctggactgg gaaaacctgg
    28081 cggcagaaaa acagacgctg gtgttctata tggggttgaa tcaggccgcg actattcagc
    28141 aaaagctgat tgaacacggt atgcctggcg aaatgccggt ggcaattgtc gaaaacggaa
    28201 cggcagtcac gcagcgcgtg attgacggta cgctcacgca actcggtgaa cttgctcagc
    28261 aaatgaacag tccatcgcta attattattg gtcgggttgt tggcctgcgc gataaactga
    28321 actggttctc caatcattaa tttaacccgg gccagagaat tctggccttc ttaatgactc
    28381 tttttatata ttcagcaaat aaaacataac cccttaataa atattatctg ctaatgggtt
    28441 atattcaccg ttacagcgac ttacaaaatt aagtcattcg ttcactgacg ccaattaaat
    28501 attcctgtat cgttcttaac gagcagtctg cttatattca tataatcaat gaatattaat
    28561 taatatataa tacataggga atgtaaatga acaaatttat taaagttgca ctggtaggtg
    28621 cagtactggc tacgttaact gcatgtactg gtcatattga aaaccgtgat aagaactgct
    28681 cttacgacta cctgctgcac ccggcaattt ctatttctaa aatcattggc ggttgcggtc
    28741 ctactgcaca gtaagtctct gacagaaatc ggctaacacc ggtttaattg cccgacgccc
    28801 tctggatttt ccgagggcgt atttgtttct atgtccttct tgcccccgcc cgtaaaaata
    28861 ttttcacttt aaattcaatt ttataactac atccattagc cacagcgcga tcctgtgcga
    28921 aattttgtga tcttcctcca cattacacaa catcatatgt tgttatattc atcatgcatt
    28981 gtcatgttgc cttttaaatg actgcaaact ctcccctaca acgtattgga caagaaaaag
    29041 gtatcgctat gggaagccag gaactccaac gcaagctcgg attttgggcc gttcttgcaa
    29101 tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa gtggcaaaag
    29161 cagcgggcac gccgtagctt acggtgctcg cgtttgtcat tggcgggtta attgtgatcc
    29221 cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc ggaaaatggc gcagattatg
    29281 tttatctgaa aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct
    29341 gggccaacga tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct
    29401 ttttaacgcc tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct
    29461 ttatgttgct acacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca
    29521 tcgccaaaat tatcccgttc actatcgtca ttggccttgg gatcttctgg tttaaagcgg
    29581 agaattttgc cgcccttacc accactgcga ttggcgcaac gggcagcttt atggcgctgc
    29641 tggcggggat ctctgccacc agttggtcgt ataccggcat ggcctctatc tgttatatga
    29701 ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc gctgattggt tcctgtctgc
    29761 tggttctggt gctctacacc ctgctggcgc tggtgatttc cggcctgatg cccttcgaca
    29821 aactcgccaa ttctgaaacg ccgatttccg acgccctgac ctagatcccc gcactcggca
    29881 gcaccgctgg gatctttgtt gccatcacgg cgatgatcgt cattcttggt tcgctttcca
    29941 gctgcgtgat gtaccagccg cggctggaat acgcgatggc gaaagacaac ctgttcttta
    30001 aatgcttcgg ccatgtgcat ccgaaataca acacgccgga tgtctccatc atcctgcaag
    30061 gggcgctggg gatcttcttc atcttcgttt ccgatctcac cagcctgctg ggttatttca
    30121 ccctggtgat gtgtttcaaa aataccctca ccttcggctc catcatctgg tgtcgtaaac
    30181 gcgacgatta caaaccgctg tggcgtactc cggctttcgg gctgatgacc accctcgcca
    30241 ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt cccggcctta
    30301 tctgcgccgt catcgttatt gctactggtc tgcctgctta cgccttctgg gcgaagcgta
    30361 gccgccagct caacgctttg tcgtaatgtt atctgaagaa aataaaatgt tggatattga
    30421 taaaagcacc gtggactttc tggtcaccga aaatatggtt caggaagtgg aaaaagttct
    30481 cagccatgac gttccgctgg tgcacgccat cgtggaagag atggtgaagc gcgacattga
    30541 tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg gcgcaaacgg cgaaacatct
    30601 ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc ggctgggagt tctgcgataa
    30661 caccccgtat cgcctcgacg atcgttgcgc ggtgattggc gtttctgact acggtaaaac
    30721 ggaagaggta atcaaagcgc tggagctggg ccgggcctgc ggcgcactca ctgcggcgtt
    30781 caccaaacgc gcggacagcc cgattacctc ggcggcggaa tttagcattg attatcaggc
    30841 cgactgtatc tgggaaattc acctgctgct ctgctacagc gtggtgctgg agatgatcac
    30901 ccgcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac gatctcaagc agttgccgaa
    30961 tgcgctcggt catctggtac gcacctggga agaaaaaggc cgccagcttg gtgaactggc
    31021 cagccagtgg ccgatgattt ataccgttgc tgcgggtccg ctgcgtccgc tgggttacaa
    31081 agaaggcatt gtaacgctga tggaatttac ctggacgcac ggctgcgtga ttgagagcgg
    31141 agagttccgc catggcccgc tggagattgt cgaaccgggc gttccgttcc tgttcctgct
    31201 cggcaatgat gaaagtcgcc acaccaccga acgcgccatt aactttgtta aacagcgtac
    31261 tgacaacgtg atcgtcatcg attacgccga aatttcgcaa gggctgcacc cgtggctggc
    31321 accgttcctg atgttcgtgc caatggagtg gctctgctac tacctgtcta tttacaaaga
    31381 tcacaacccg gatgaacgcc gctattacgg tggtctggtg gaatattaat ccctctcccg
    31441 gcccggcaac gggccggatt tacgcaagga gttacccgat gaaaacaggt atgtttacct
    31501 gcggccacca gcggctgcct attgaacacg catttcgtga tgcaagcgag ctgggttacg
    31561 acggcatcga aatttggggc ggtcgcccgc acgcgttcgc gccggactta aaagcgggcg
    31621 gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat gccgattatc ggttacacgc
    31681 cagaaaccaa cggctatccg tataacatga tgctgggcga tgaacatatg cgtcgtgaaa
    31741 gcctggacat gatcaagctg gcgatatata tggcaaaaga gatgaacgcg ggttatacgc
    31801 tgatttctgc ggcccacgcg ggctatctca cgccgcctaa cgttatctgg ggcggctggc
    31861 agagaactta agcgaactgt gtgagtacgc ggaaaacatc ggcatggatc tgatcctcga
    31921 accgttaacg ccgtatgaat cgaacgtcgt gtgtaatgct aatgatgtgc ttcatgcgct
    31981 ggcgctggtg ccttcgccgc gcctgttcag catggtcgac atttgcgcgc cgtatgtcca
    32041 ggcggaaccg gtgatgagtt atttcgacaa actgggcgat aaattacgtc atctgcatat
    32101 tgtcgacagc gacggggcca gcgacacgca ttacattcct ggagaaggca aaatgccgct
    32161 gcgggaactg atgcgcgata ttattgatcg gggctatgag ggttactgta cggtggagct
    32221 ggtgacgatg tatatgaacg agcccagact ctatgcccgc caggcgctgg aacgctttcg
    32281 cgcgctgctg ccggaggatg agagatgaaa accctagcga caatcggcga taactgcgtc
    32341 gatatctacc cgcaactgaa taaagcgttt tctggcggta atgcggtcaa tgtggcggtg
    32401 tactgcactc gctacggcat acagccggga tgcattacct gggtgggtga cgatgactac
    32461 ggcacaaagc tgaagcagga tctcgcccgc atgggcgtcg atatcagcca tgtccatacg
    32521 aaacacggcg ttaccgcaca aactcaggtg gaactgcacg acaatgatcg cgtttttggc
    32581 gactacaccg aaggcgtgat ggccgacttt gccctgagtg aagaggatta cgcctggctg
    32641 gcgcagtatg acattgtgca cgcggcaatc tggggacatg cggaagacgc attcccacag
    32701 ctgcacgctg cgggcaaact taccgctttc gacttctccg acaagtggga cagcccgctc
    32761 tggcagacac tggtgccgca tctcgatttt gcctttgcct ccgcaccgca agaagacgaa
    32821 gcgctgcgtc tgaagatgaa agcgattgtt gcccgtggcg caggaacagt gattgtcacg
    32881 ctgggtgaaa acggcagcat tgcctgggat ggcgcgcagt tctggcgtca ggctcctgaa
    32941 ccggtgacgg ttatcgacac catgggtgcc ggagattcgt tcattgccgg attcctttgc
    33001 ggctggtctg cggggatgac attaccgcag gcgatggcgc agggaacggc gtgcgcggcg
    33061 aaaaccattc agtaccacgg tgcctggtag gtataacgtt ggcgtgagca tcttcacgcc
    33121 aacgtgctgt tacttgccgg aaaacgaccc tataatccga gtaattcatt ctttatttca
    33181 gggtcgatta tgtcagctac ggaccgctac tctcatcaac tcctctacgc taccgtccgc
    33241 cagcggctgc tggatgatat cgcgcagggg gtttaccagg ccgggcaaca gatccctacc
    33301 gaaaacgagc tttgtacaca atataacgtc agccgcatta ccattcgcaa agccatcagc
    33361 gacttagtgg cagacggcgt actgatccgc tggcagggaa aaggcacctt tgtacaaagc
    33421 cagaaagttg aaaacgccct gcttactgtc agtggtttta ccgattttgg cgtctcacaa
    33481 ggcaaggcga cgaaagagaa agtgatcgaa caggaacggg tcagcgccgc gccgttttgc
    33541 gaaaagctga acatccccgg aaacagcgaa gtgttccatc tctgccgggt gatgtatctc
    33601 gataaagagc cgctgtttat tgatagttca tggatcccgc tgtcgcgtta tcctgacttt
    33661 gatgagattt acgtcgaagg aagctccacc tatcagttat ttcaggagcg ttttgacacg
    33721 cgagtggtca gcgacaaaaa gaccatcgat atctttgccg ccacccgccc gcaggcaaaa
    33781 tggctgaaat gcgaactggg cgaaccgttg tttcgcatca gcaaaatcgc ctttgatcag
    33841 aatgacaaac cggtgcacgt ctccgaactc ttctgccgcg ccaatcgcat caccttaacg
    33901 attgataata aaagacatta accgtaggcc ggataagatg cgccagcatc gcatccggcg
    33961 atgctggcgc actgaatttt acatcccgca cgttcccctc accctaaccc tctccccaaa
    34021 ggggcgaggg gagcgttcag tgcggaattt agcttgattg ccgaacggtg ctatccagca
    34081 ttaaatactc gcaatactct cacgcaaaat ccgcagcacc gtctcttcac cgctgcgatt
    34141 cgggttaatg cgaatcgcac aatgttcgag ttgtggattc gcctggcgaa acgttcctga
    34201 aagtcgatag aagagcggcg ggatttcata tttcgactct gcaccgaccg ggtaaggcaa
    34261 cgcgccacgc ttttgcgctt cttccagcac cctggcggca atcagctgat gaaactcgac
    34321 aatcaacacc ttcgactgcg cattggcaat gacggcgctt ttcacttccg ccaccgcgcc
    34381 gccgttaagc aaagccagca accgttcgga tactcccgcc tgcaccgcat gcatcactgg
    34441 cgcgaacacc agaccacgca agacttccag cgcctgtgag ccctggatct ggctaccgcc
    34501 ggagtaaagc gtggcgcgaa tccgactaat aacatcggca tcgccaacca ctgcaccaac
    34561 gccctccggc ccaaacagct tgaagcagga gaatgtcgag acattcgcgc cgcattcaca
    34621 gccgattcgc gctaccttca tcaccgcata gttatcatcg gttaacgctg gaacacctgc
    34681 cgcgcgcaac gttgccagca catctgccag cacatagctg tcctgcggct gctggcgcgt
    34741 atgctgcacc agcgcggcat caggttgttg ctcgtcgacg acctgcttca gtgccaacag
    34801 gtcattgaaa tcagcagtaa taagcgtcag ccccatctgc tcaataataa cccgtgtcgt
    34861 cgggtaaaca ggcgcgtcat gcaccagaag acgctgcccc ggtttgagca aagccgccag
    34921 cccggcgcga atcgcgccag tccccgcgcc ctgcaccagc gccgcggcct gtgcgtgaaa
    34981 tgcatcagcc agcacctgct ccacccgttg ggtaatacgc ggttgattca gacctggagt
    35041 taaccctaaa tcaccgccag caagaaactc gctgcctgga aaatggcggc aaatagtgtc
    35101 caccagcgca aactgctttt gctgcgcttc agcaagagtc aggctttgca aaggaaacgt
    35161 cttcatagga gccttcctta agccggaacg aacaggccaa gccagtaaag aacattcagc
    35221 agaataccgg tgatcattac tgccacgacc ggtgccgcca ttttctgtac cggacggccc
    35281 agggattcgt tgaggaagta aatggcaacc gcgatagaga atccggtata acccgccatc
    35341 ttaattgctg cgaaaatcga accgaccagc agcgccactt ccatcagcat attcatggcg
    35401 ttacggatgt tatccgacgc attacgcacc gacgggtagc gtcccagcca tttgccgatc
    35461 gaacgaagca gcaagacttc cgccgaaata accactgcgc ctaataccgc tgcaaccatc
    35521 ggattcggcg agagatagcc caccgcataa acaaaggtaa agcccgcaac tgcatacaca
    35581 cccgttgcta aggcggtggt ggcaatcaac ggcacaaacc ccagtccgcg cataaattct
    35641 gccagagccg cctgattaat cagcgtttgc gattgttccg gcgtcacgcc tgcggaatac
    35701 gctttctcca gtgtgaagat cgaaacttcg ctaccagcaa aaatcttcat actggcaacg
    35761 gcggcaatca atgctcccac gatggcgata taaggtaagt ttttgataat ccgcgacgtg
    35821 cgctcttcaa acaccgaaag cccgctggca tcgatgtcat tttcatcacg atgacgcagg
    35881 tcgtgagtta tcgcgatccc cagcagcatc accatgccaa taaagatttc aatggattca
    35941 gggttaagat gtgggaaata gcgcacgact accacacggg tcatcagaac aaccacggcg
    36001 gcgaccagac tttgcttcca gccaaactgg tagaaaatcg ccaccagcgg gaacagtgca
    36061 aaagcagaaa ccaccggcga gcttaattcc ccgaggctac ctaatacatc caccggcagc
    36121 gcggtcagca gctggttaac tggcaacagg caagtaagga tcaacacgcc ccagatagcg
    36181 ccaagaccaa acgccatcag gctgtttatc gccagtacgc cgaggatatc ggtaggaaga
    36241 aagagtaacc atgcgttgag cagccaggtt ttcagggtaa aagagatccc caccgaggcc
    36301 acaaaaccaa tgctcagacc aaaagcgata ctccccgcct cgcgacggtt catatagcct
    36361 tcaatcagtt gcggcaggat cgggtggatg ccgtcatgaa aaacagccgc cgagcgatgc
    36421 gccagaagcg atgtcatacc cgtcaggcac gccaccacga taatctgaat atacagatcc
    36481 atagtcgtgc cttttatttc agatggttaa tcaacatcgg aatcgcgtgt tcgacgtgct
    36541 caacggaaag gccaaacgcc actttccctt cagcgatcat tttggcgata tgctcgtctt
    36601 tcgctttaat gcctggtttg gcaatggtgc aacttttgtt atagccaatc accgcgatgg
    36661 caatcgacaa tgccgcgccc gcaccggtat tacacgcgcc aatgtaataa tctagttgcc
    36721 cggatttcac cttcattgcc gcttccatgt cgttgtgaat gaaaacttca aaacagccag
    36781 gagccgtcgc ttcaatggtt tttttaatct gctcacgctg taagcctgca acgccaatct
    36841 ttttcatgag tctgtcctta ttggaaaaat tgagagggat tttcacgtaa catcacatcc
    36901 acatcggcct gactgaatcc tgactggcac aattgcggaa taaaggtggt caataaatag
    36961 tcatagccat aaccaccatt ggcttttaaa tgggagcggc gcgtaatatc catcgacagc
    37021 atgacgcggt tcagcaaccc acggtcacgt agcgcgtgga gcatcgcaat gcgcttttcg
    37081 tccgggtagt aactgttctt accaatggtg tcgaactgca cgtacgcgcc gagatcgatc
    37141 atcttcaaaa tgttgtcgag gttgtctttc agatcgcagt gaccaacggt gacgcgcgaa
    37201 agatcaactc cgtgggcttg caacagagcc agttgttcca gccccatggt gctgaacgac
    37261 gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca gcgcagcggc aataaatacc
    37321 ttctcttcca gcggcgtaat ctttccttcg ctggagccga tctccgcgat gatcccggct
    37381 ttcagctccg tgccatcgat accctgttca atttcatcga ccatctcctg caccagttcc
    37441 tgcacgctgc gggtcaccac atgttccggg aaaaacgcat cctggtaata accagtacag
    37501 gccaccacgt tgatcccggt ttcgcgcatt acatcgagca taaattgcgc attgcgcccc
    37561 atgtaacggt tggtcatctc aatcacatta cgtacacccc gggtcatcag gtcgttcatc
    37621 tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca cgttgttttt aaagccggag
    37681 agatcaatat gcagatgctc atgggccagg gtgtaacccg tcggatcaaa actcataggg
    37741 tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc attttgtggc ggtggcgcac
    37801 ggaaaaattc acacacggta gctcccacat cagaaagcgt ggtgcgcaca ccgagctgcg
    37861 tggcgaccag cccttgctga taaaccagca ctggcaccac ttcacgggta tggtggctgt
    37921 gaccaatggt cggatcgtta ccgtgatccg ccatcacgac caggcaatca tctggctgca
    37981 tcgcctcaac aagccgggca aggttacggt caacgacctg caaacgttcg gcataacgtg
    38041 cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat attgatgcaa ataaacgccg
    38101 tcggatgggt gttaaattcg tcgagggtga tatccataat ccgctggcta tccaccaggt
    38161 tttgccagct cacaccataa ggattgctga cgatatctgc caccttaccc accagcacgg
    38221 ttggcacgcc cgcttcatac agtttttgcg gcacctgcac tttttcatca acaccgtagc
    38281 ccatatgcac gacctggaag ccgttgtcat aagcgccaga acgcggcgca ttaataccaa
    38341 taaagcgccc ttctttgctt tctgcggcat cgagaatacg ctgactgttg gttaacaggc
    38401 cgccaaatgt aatgacccga ccgacctgta cctggtcacg cacgatacga ccaattttga
    38461 ttgcgtcgtc aaaagagatc acagagagat tggcggtaat gttatagact tggcctaaat
    38521 ccgcctcgag attatcgcca atcgcaaccg cctgattgac ccacagaaat tgcagatcat
    38581 cgccacggcg ctccacctgc cagccagcgg aaactaatgc ctgctcaaca cggtcaatca
    38641 catcgcgaaa aggcatccgc agcggcggta acgggcgcgt gcctaaaatt tcctgatgcc
    38701 ccataaaggt atcgccacct tcatgttgca gctctgccac gccccaggtt gcggaatctg
    38761 acggctgcat atcgcctggc gcataaccca atgtgttgat tagccccagc ttctccaacg
    38821 ttggtagctg caaatgcggc aactggctca ggatgtgacc acaggtattc gccccggcat
    38881 cttgcggacg caccagcgtg acatctttca ttgcccctac gccaaagcta tcaatcacta
    38941 acaccacaaa tcgcgccatt tatgccttca gagggttgcc gaggctgtca taacgaccaa
    39001 ctatttctgg ttcgccgcgc tgaataccgg acaccagcac cacatcgcta cgggtgacaa
    39061 aaatctgcgt gcgaaaacag agcaccactg cactgctcac cggatactcg cctgccagcg
    39121 gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca gtaatctttt
    39181 gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc acgacggtag taaccgccgc
    39241 cgtagcagta gctgtcgcca cggaaatgat gggagatttc acttaaccag agcatcgcga
    39301 tacgttcagg ctgatcgccc tgctggtttg ccggaatagt accggtcagc gcatgaccgg
    39361 gttcggcatg agtcgcaccg tattccgcca gcaatggcag cgaagtgcag ctggtcgctg
    39421 aaggtacgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc cgtgcttgta
    39481 ccagcgtgtg aagattcggt gttggcaaaa ctttcccggc agcctcatcc caaagcaggc
    39541 aagggaaatg ggttagtccg gctaaatgca gccccggcag tttcttaatt tcagcaatca
    39601 cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc cggataaaga aaatcatcgt
    39661 cgctataaac tttgagcagc acaggctgga cccgtccggc cttaaccgct gccgcagaaa
    39721 cttcccgcgc tttgtcgaga gtaaacacgg tgatgatgtc ggtgccctgt tcaacggcgt
    39781 cagcaacctg atgacaaggg atttgtacca gatgcccctg atgcgccact ggcaaaccag
    39841 cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg tagcctaatg
    39901 ccagcaattt ttccgccagc cacggattac gaccaaattg cttggtcatc agatacagtt
    39961 caatgccgta aagccgcgcc gtctcaatga gccgcttacc gttttccagt atctgatcca
    40021 cgtcgatcac ccagctgtcc ggggcgattt tgccctgctg ccacaaactt agtgcggcgg
    40081 aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg cctcgtttct
    40141 gttccttgaa ttaaatattc atttttttaa atatttaggt tgtgcgacgt ggatttgtcg
    40201 ggagcttcaa acctcttcgt tagcagccat ccataatcca taaagattgg ccagaaaata
    40261 gccttcttca cacgggttaa cttccagagc gaattccttc aacaacacct gatgcaattg
    40321 cacaacggct tgccagtggc tggattgcgc cagttccgcc agcaactcgt tatccagcgg
    40381 ctctatttct tcaccacggc gactgcgcat cagtgcactc gccatatgtg tcatcgccat
    40441 cgttccttgc tcactgcgca ccggcagatg gcactctttt tccagtacgt tgacgacctg
    40501 catcatgcct ttgcagacgt ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct
    40561 ggtttccata tcagatgcct ttcgtttaat aactgggttc ctgttcccca ctcaccaccc
    40621 gttgttgatg ggcaagcagg gcgtgtttat ctacaacggc acgcagcaat tgttgcattg
    40681 ggtaatcatc gactcgcgtc aggaccacgg cttcggtggc cagtaaaaat cgcggatcgt
    40741 ctgttagcgg cgtcgcctct aatcccagca tggtcagttc gttttccgcc accacgttcc
    40801 agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga taagagagat
    40861 cgactcgttc cacatcacta tcgccaaaaa agacatcggt catgattttc tgatccgccg
    40921 aacgactatc gagacctact cgcttaacat ttccagactc acctttgcgg catatcagtt
    40981 gatgttcacc aacatacgta tgcgggccaa gctcgagggc gatgcacaaa ttgttttggc
    41041 tgagataact ttccgccgcc aggcgggaaa ctactgccat gtcatagaca ccgttaagta
    41101 aacactccac gcgaatatcc gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa
    41161 actgggcttt caatccgctc gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg
    41221 cacataccac gttgttgata tccacatgac tcagcaatgc tttgttatcc atctcgacaa
    41281 gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat tccagcgttt
    41341 tcaacgcggc ctgcgtcagg ccaaccgatg aacggcattc agttgccagc tcatctatgg
    41401 ttttcagtcg attaccgcat ttttcaccca acaaataacg ggccagtgtc gtgatgacga
    41461 cgccttcttt tttgataaac gttcgacgca taatatattt tcaataaaat gaatatttat
    41521 atcttcatta aaatgaagat aagtggcgac gacgagatgt ggaaacggcg aggcgcttca
    41581 cattttttcg ggttgttgcg aaaagaggat aaaaaaaccg ggtttccccg gtttcagagt
    41641 gatgacgaag tgatttattc gacaatgcga gcaaagggta attcccactg gcgcttgcct
    41701 gctcccctca ccctaaccct ctccccaaag gggcgagggg accgtccgag cgttttgata
    41761 ttacggcttc gccacaaaac caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg
    41821 tacactggct ttttctgcgc cgtctttcat cacctgttgc aggaaggctt catcgttgcg
    41881 gaaacggtga tagcgttcct gcaattcagt cagcataccg gaaacggcat cagccacttc
    41941 acctttcaga tgaccataca tcttgccttc gaactgtttt tccagttctg ggatgctctg
    42001 gcccgttacc gcagaaagga tatccagcag gttggaaacg cccgctttgt tctgcacatc
    42061 gtagcgaact accggcggct cgtcggagtc agtgaccgcg cgtttgattt tcttcactac
    42121 cgatttcgga tcttccagca ggccgataac gttattgcgg ttatcgtcag acttggacat
    42181 cttcttggtc ggctccagca gcgacattac gcgcgcgcca gatttcggaa taaacggctc
    42241 cggcacctta aagatctcgc catacagcgc gttgaaacgc tgggcgatat cgcgactcag
    42301 ttccaggtgc tgtttctggt cttcacccac cggtaccaga ttagtttgat acagcaggat
    42361 gtccgcagcc atcagcaccg gatagtcaaa cagaccagcg ttgatgttct cggcataacg
    42421 cgcagattta tctttaaact gcgtcatgcg actcagttcg ccgaagtagg tatagcagtt
    42481 cagtgcccag cctaactgcg catgttctgg tacgtgggac tgaacaaaaa tggtgctttt
    42541 ctcaggatcg ataccacaag ccagatacaa ggccagcgta tccaacgtcg ctttacgcag
    42601 cttctgtgca tcctggcgca cggtgatcgc gtgttggtca acgatacagt aaatgcaatg
    42661 gtagtcatcc tgcatgttta cccactgacg cagcgcaccc atgtagttac caatggtcaa
    42721 ttcacctgag ggctgtgcgc cactaaaaac gatgggctta gtcatttttc gattcctgat
    42781 tttcgctatg cggaagccct aatgcgggca gaaggtcatt tatagactga taaattacat
    42841 caggctggct gagatcgata gcctcgccgt agttatatcc gtaggttaag ccaactgatg
    42901 ggcaacctgc cgcttttgcc gcctgaatat cattgcgtga gtcgccgaca aacagcatct
    42961 gttgtggggc aattcccatc cgctcagcca ccagtaacag cgggtccgga tgcggttttt
    43021 tgttttgcac atcatcgccg ccgataacca cgctaaagta tttggcaata tctaaggctt
    43081 cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt
    43141 tagcctgcaa cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg
    43201 caacctcgcc atagtagcga tcgaacagtt tacgcagaat acgtacctgt tcttctgccg
    43261 gaatgtcgtc atcaacgggc ggtttcccca ttgttttacg cagagtcgca cgttcctgac
    43321 gcgcccaggt caatgcgcgc tccatcagaa catctgcgcc gttaccaatc caggtaataa
    43381 cgcgttcttc acctgcgacg ggcaactcca gcgcatacag cgccatatct accgcagcag
    43441 caagaccagg agcactgtcg accagcgtac catcaagatc aaaagcgacg ccgcgaatat
    43501 cttcaaactt attcatgact tacctttgcc agttcactgc gcatttcatc aatgactttt
    43561 ttatagtctg gctggtcgaa gattgccgaa ccggcgacga acatatccgc gcccgccgca
    43621 gcgatttcgc caatgttgtt caccttcacg ccaccgtcca cttccagacg aatgtcaaag
    43681 ccagactcgt cgatacggcg acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa
    43741 gactgaccgc cgaaaccagg gttgacggac atcagcagga tcacatccag cttatccatc
    43801 acgtaatcca gatagctcag aggtgtcgcc gggttaaata ccagacccgc tttacagcca
    43861 ttttctttaa tcagttgcag cgtgcggtca acatgctcgg aggcttctgg atgaaaggta
    43921 atgatgctgg caccagcggc agcgaaatca ggcacaatgc gatcgacggg tttcaccatc
    43981 agatgtacgt cgataggggc ggtaatgccg tagttacgca aggatttcag caccattggc
    44041 ccaatcgtca gattgggaac atagtggtta tccatgacgt caaaatgcac gacatcagcg
    44101 ccagctgcca gggcttttgc ggtatcttca cccaggcggg caaaatcagc cgacagaatt
    44161 gagggggcaa tcaaatactg tttcatccgc ttctccttga gaattatttt ttcgcgggtg
    44221 aaacgactcc tggtttgtac aaagccagca gttcgtccac ctttttacgt gtgccgccgt
    44281 tgctgcttat actgcgtcga actttgacga catgcaattt tgcgcgctga taccactcac
    44341 gcgttaacat cgtatcgtga ttggagatca gcactggaat atggcgctca accagacctt
    44401 cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg
    44461 taaagttggc ggtcgcagac agcggtgcat aaggcggatc gcaatagacg acggatgcat
    44521 catctgcgcg ctccatgcta tcggcgtaag actcacaata gaaaaaggca ttctgcgctt
    44581 tttcagcgaa gtgatacaac tctgcttccg ggaaataggg ttttttgtag cggccaaacg
    44641 gcacgttaaa ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg tagcggttca
    44701 aatataaaaa cagtaccgcc cgacggaacg gatcctggct tttgttgaac tcttcgcgga
    44761 actgatagta aacctcggcg caatttgttt cgggaacaaa cagctcgcgt gcggcctgta
    44821 cgtactcatc agtacgcatc ttcacaatgt tatagagact gatcaggtcg ctattgatat
    44881 cggcaaggat ataacgagaa aagtcggtgt tgagaaacac cgacccggca cctacaaaag
    44941 gctcaaccag acattcgccc ttgggcaaat gccgtttaat atcatcaagc aggggatact
    45001 tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat gccgactaac taattacacc
    45061 ttctccggct gtggagaaag ctccgacagc atccagcgct tcaggcagct cccgcaacga
    45121 taaaccattg cgggagataa ccttgattac ttcagatcgg cctgtacctg acgcagcggt
    45181 ttcgcccacg ggtttttggc ctgaacatct gctggcaatg tagataccgc ttttttcgcc
    45241 tcttctttcg aagcgtacac gccagaaacc aggacatacc acggctgacc attacgcgtc
    45301 gtttcataga caacgtagtt tttcagattc tctttcttcg cccaaccgtt caggttgtcg
    45361 tagttagagg aactgctcag ctgcagagtg taatggctgg acggtgccga tttcaacgaa
    45421 ccaacattac ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg cgccggggat
    45481 gccgtctgta ctggagccgt agtcgccgtc tcttttggtg ctggcgtaga agtcgcagcc
    45541 ggtgccttcg tgctcgccac aggggcagct ggttcagtac gcttcggcgt ctgtgctacc
    45601 ggcttcggct ccgttttcac ggttgcttgc ggttttttcg gttcaatcac cgcctgctga
    45661 cgaactgggc gcgtagtgga cggacgttca gcggtttgcg ttttcgccgt gtcacgcgat
    45721 gcattgccat tgcgaacagg cgcaaccgtt gcgggttcag tcggcaacgt ggaattgacc
    45781 gccacattgt tcagctgttg ctgattttgt ggctgggtca gcgcattgtt caggtcaccc
    45841 tgcacttcaa cacgttgttg accatccgtt gccaccgggg tttgcccttg agtcggcgta
    45901 gaagagatcg gtggcagaga aacatcctgc tgagtatttt ccgcagacgt ggttcccggc
    45961 gctggctgca cgccattcgc ctgatcggtc gcattgccag caagatcaat actcttctcg
    46021 ccagacgcgg tttgatcgct ggaagtggtc gagggggctt ttagcgcaga accgataccg
    46081 atgatcaaca gcagtagaac cagaatgccg acgcccatca tcatatactg acgagaagcg
    46141 ggtttactgg ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg
    46201 gtttcatctt cggattcgtc aatttcttct tcgatttccg gttcctcatt gcgctctttt
    46261 tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt caatatcatc aaaattgatc
    46321 tgcggttcgc cacgttcagt acgctcagaa cgttcagaag attgacgaga acgaccagta
    46381 cgacgatcgc tgggatcggg tttcagctcg tcttctggtt tgaattcatc catttaacac
    46441 cccactaaaa ggttaatgct taccacgttg caattaacct gaagctaata gaccgcttga
    46501 taagcggcct gacctttctt gttgttacgc tgattgacaa tcggcaatgg cgttaagaac
    46561 aagctcgtgc gaaacgccgc tgcgaacttc actcttacca attgccaacg gaagaattaa
    46621 gcgcatctct cccgcaagga ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc
    46681 ggacatttcg cgcggcccat tgaccggtaa cccagcccgc gtgagcaggg ttataatacg
    46741 ctgcgtttcg gcagaactaa actgcccgag acgttccgac gtccgcgccg ccatcaccat
    46801 acccgcagcg accgcttcac catgtaacca attgccatac cccatttcag cttcaatggc
    46861 atgaccaaag gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc
    46921 ggcgacaact tctgccttca gttcacaaca acggcgaata cagtacgcca ttgccggacc
    46981 gtccagacgc aacaacgcat ccagattctc ttccagccag ttaaaaaacg caccgtcaag
    47041 aataatgccg tatttgatga cttctgccag ccccgacgct aactcacgcg ggggaagcgt
    47101 tttcagacag tcgagatcca ccaccactga agcaggctgg tagaacgcgc caatcatgtt
    47161 tttaccgagg ggatggttga ccgcagtttt gccgccaacg gaggaatcga cctgcgacag
    47221 taacgtcgtc gggacttgaa tgaaacgaac accgcgctga taactcgccg ccgcgaagcc
    47281 ggtcagatcg cccactacgc cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg
    47341 cttttgtaac aacgccgtaa agacggtatc gagtacagct aggcttttat actgctcgcc
    47401 gtcagggagg ataacgctat cgacgttaac acccgcctgt tcaagtacgc cgcggacctt
    47461 atcgagatac agaggagcca gggtttcgct ggtgaccaac atgacctgct cgcccgattt
    47521 cagcggtaag aatgaagctg gttcattaaa caaaccagat gcgatggtaa ttgggtaact
    47581 acgttccccg agagtaacga caatcctctc cataacgcga catccacctt aattactgta
    47641 cccgcagacg agtgtatata aagccagaat tagttgcttt ccagcatgtg aataatctgg
    47701 tttgcaacca ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc aatctcttca
    47761 tacagcggat tgcgttcatt ggccaacgct tccagaactt cacgcggcgg tgtttcaacg
    47821 tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt
    47881 tcaagataaa cgacaacgcc acgagcggaa agacggttac gcgtttcacg ggatttcaca
    47941 gagccgccgc cagtagccag cacaataccc tgtttctcgg tcaactcatt gatgaccttt
    48001 tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc cacatcagct
    48061 ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag tttttgagct
    48121 aactggcgcc caatagtgct ttttccggca cccataggcc caaccagaaa gatattgcgt
    48181 ttctctgcca ttttttcggt actactaaga ctattcgtta atgataaacc cgcttcgctc
    48241 agagagcgcc gcaggacatg aactgaaacc tcataagata ttgcgagagt cagactgaaa
    48301 attatctcaa tactccagcg ggtttggcaa ctgaataaat cacccatccc tctgcatatc
    48361 tggtcgctgc aagcgcactg ccttgctacc accgctctgg cgataaatca ccgggtaaga
    48421 ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa atcatcatca tggaacgcag
    48481 gcagcgaaga gcaccaactc tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt
    48541 caaatagatg aaacacgttc agcataaaaa cattaccgct tttacggctg tttactcact
    48601 ggaaaccagt cgtggcgtga taaacaccac taactcgcgt cgttcatctt cttttccgtc
    48661 atgacgaaat aattgcccga accagggaat gtcgccaagc aacggtacgc tatcctgacc
    48721 cgatttattt ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc tttttacctc
    48781 gacctgcgtt tcgatctcct gcttatcaat cgccagcact tcgccatcgg cctgctgtag
    48841 cacctgcccc ggaacgttct ggctgatgtg taatttcagc cggatgcgac ctttttgtaa
    48901 caccgtgggc gtgacctcca tccccaggac ggcctcttta aattccaccg acgtcgcgcc
    48961 actttccccg ctggaagcct gatatggaat ttcgctcccc tgtttaatgc tggcaggctg
    49021 aagatgtgag gccagcagac gcggactggc gataatatcc agctgctgtt tttgttcgag
    49081 cgcggaaagc tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg
    49141 cgttgtcgcc gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc caacgccacc
    49201 agcgtgttgc gcatcggcca gcgtccattt cacgcctaac tcacgcaaac ttttttcatt
    49261 aatggtgaca atatgcgccg acagctcaac ctgcccgacc ggcagatcca tttgcgctac
    49321 ccactgttca agcgcgctta acgccgtttt gttatctcgt agcaaaaggc gattggtgcg
    49381 tttatcgacg gtcatactcc ctttggcact cagtagcttc tcccccgctt tcgccagttc
    49441 tcccgcgtcg gcgtattgca gggttatact gcgattttcc agcggcagat ttgcctgcgc
    49501 ccgcgcctgc tccgcctcct ggcgggcgat attgtcattc tgccaggcaa tggaatgcac
    49561 tgagagaatg ttgccttcct gccgcgttat cagtccggcg cttttcacta cagtttgtag
    49621 tgcctgcttc cagggaacat ctgttagatg taacgacacc gtaccgctga cgtctggcga
    49681 cacgaccagg ttcaacttct cctgttcagc cagcgcctgc aacacctgag ctaccggaac
    49741 gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg gtatcagcat
    49801 caacagtagt gcggctatcc attgcttcat ttgtatctcc ttgccgttgc cacaaccatt
    49861 gtggcggttc gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa
    49921 ttgtccagcc gttttccagc acatcgtttt gctgcacccg tcgccatttc ttttgcccgt
    49981 cttttattac accgatgatg cgctcgcctc gccctaccat cccctgatag cgccactggc
    50041 taagttcgct aatccggcat agatcttccg gcgatttaaa agggtcacgc ataccggtta
    50101 aaaggcacaa tgcaatacct gccaacaacc agcgtttaac cctcatgcgg catctccagt
    50161 tgtagcgtga acaaaagatc atcaccttcc acgcttaacg aaaaacggct cacgctgacg
    50221 ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc
    50281 aactcgcctc cctgcgcgga tggatgccag gaaaccagtt gcgcgccgga taactgaaaa
    50341 tccagtggcg aaaagggcag cgttttttcc tcgctgaagg gagtggtgtc taccaggcga
    50401 tacagtgcgg cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct
    50461 tcatggtgtg tcgatgatag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag
    50521 caaaactggc ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct
    50581 aaccttcctt gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc
    50641 tccccgctga ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa
    50701 cgcggtaatg cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt
    50761 tgtcagccag gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc
    50821 gcgggtaaat tgtcgctggc gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc
    50881 cagcaaacgt ggcttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa
    50941 aactgcgtct atgcgcgctt cggcgctgcc tgtcagacgc agtattagcg ttatcccgac
    51001 ggccagcagc agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag cggtccggcg
    51061 ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc agtacgcctt cccaagcgcc
    51121 agccccagcg cgatggcaaa gtctccacca ggcggcggta gcggcggctg acgaacagaa
    51181 acggcctccc aggggtcaaa tccgccttcg ccacatatcg cgacgctgtc gggatcaacg
    51241 gataacgctg ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag
    51301 ctatagcgcg tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga
    51361 gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcactaaca
    51421 tgaacacgca acctttctgc cagcgtaagc agcgttgcca gctctttgct ttgcgcggca
    51481 gtcacgttat aagcggggct gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga
    51541 tccatatcca gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcacca
    51601 agggacatcg acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac
    51661 ataatgtgat gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagctgc
    51721 tgcgcatcaa caatccgccc atctttgata atgtcgtgcg ccagcggcaa ccgccaccag
    51781 cgttgcaaaa agcattcttt tgcgccccgc acgatcgcaa ccgctgccgc ttcttgctgt
    51841 tgtaaatgca aaccaatttg ccagatctta aatgtcattg tgatgatctc cttatcaccc
    51901 gtcactctga cgggtatatc aatgcgtctg gcttgccttt atactaccgc gcgtttgttt
    51961 ataaactgcc caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt
    52021 gatccttgca gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta
    52081 catcgagcca caactgccgg atgtggtgac attaaaagat gttcgcctgc aaattccgat
    52141 gcagatttac agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc
    52201 ggttacgttg gatcaaatcc caccggagat ggtgaaagcc tttatcgcga cggaagacag
    52261 ccgcttctac gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc
    52321 gctgttctcc ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa
    52381 tttcttcctc agtccagaac gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat
    52441 tcgcattgaa cagttgctga cgaaagacga aatcctcgag ctttatctga acaagattta
    52501 ccttggttac cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt
    52561 cgaccaactg acgctgaacg aaatggcggt gatagccggg ttgccgaaag cgccttccac
    52621 ctttaatccg ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg
    52681 gatgctggat gaagggtata tcacccagca acagttcgat cagacacgca ctgaggcgat
    52741 taacgctaat tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt
    52801 gcgccaggag atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta
    52861 caccaccatc acccgcaaag tgcagcaggc cgcgcagcag gcggtacgta ataacgtgct
    52921 ggactacgac atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga
    52981 gtcggcgtgg gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc
    53041 gctgctgcct gccgcagtca ccagcgccaa tcctcaggga gcgacggcga tgctggcgga
    53101 cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga
    53161 tactcagcag ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat
    53221 ctgggttcgt caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc
    53281 gctggtgtcg atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt
    53341 caatcagagc aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa
    53401 accgttcctc tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga
    53461 tgtgccaatt tctcgctggg atgcaggtgc cggttctgac tggcagccga agaactcacc
    53521 accgcagtat gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt
    53581 gatggtacgt gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt
    53641 cggcttcccg gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt
    53701 caccccaatg caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctagtgga
    53761 cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc
    53821 gaaagtagcc tgcccggaat gcgatattcc agtgatttac ggtgatacgc agaaatcgaa
    53881 cgtgctggaa aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc
    53941 tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca
    54001 ggagtacgca ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa
    54061 caccaatatc tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt
    54121 gcagcgtcgc gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt
    54181 ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg
    54241 taatctcggt catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg
    54301 cggtgccaag agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt
    54361 gccggagcag ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac
    54421 cgggcagtta gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc
    54481 gacacaacag gcggtgcacg aggtgggaac gaccattatc gataatggtg aggcacagga
    54541 attgttctga ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt
    54601 ttatgccgga tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat
    54661 tgcaggaacc ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac
    54721 tcgcccctgc cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc
    54781 gttgaagtca gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg
    54841 tagcggctct ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat
    54901 attcatttta ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc
    54961 gttcgcacca aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc
    55021 acccggatca attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta
    55081 ttcgcggatc aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt
    55141 ggttggacgc attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacact
    55201 ctcgacggta aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg
    55261 taatgatttg ttcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg
    55321 acacgctttc ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc
    55381 gtcctcaaat caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc
    55441 cgccttcaga gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt
    55501 taaactccac gcttgccgat agccaaccgc agaatcatgt attgtgtccg gtgcgactga
    55561 ccacgcctga cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc
    55621 tgctttgctg gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacagcg
    55681 tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga
    55741 acgtagcgcc gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc
    55801 aacgggagct agcgcggcac cgatctctct ggtgctgaat gccgaaagca acaacgtcat
    55861 gatgctgaca cacgctatca cacgttacgg catctctacc gacgatccga ataaatggcg
    55921 ttactacctc gattcggtag aagtccacct gccccccttc tgggaacagt acatcaacga
    55981 tgagaatacc gttgaactga ttcataccga ttcgctgccg ttggttattt cactcaacgg
    56041 tcatacgctg caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc
    56101 cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa
    56161 agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat
    56221 cgtcgcctcc ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg
    56281 gctggcaggc ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc
    56341 ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt
    56401 gtttggcgaa aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt
    56461 ctatcccgca cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga
    56521 tatctatctc gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt
    56581 aaaaaacttt ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct
    56641 ggtgctgttt atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc
    56701 atggatgaaa ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg
    56761 cgtgcgggta ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc
    56821 cggaacctgg agcgcgaaaa ccaattcacc ttttttacca tttgactgct cgcagatcat
    56881 ctggaatgac gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc
    56941 attgactgaa gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag
    57001 tgcctcatta cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga
    57061 cattgtactg aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa
    57121 tgcgctggtc aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc
    57181 tgggaagctc gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa
    57241 cctggtggca acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc
    57301 actaaacagt ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt
    57361 tgatcagccc tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc
    57421 tttccagaaa ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac
    57481 aaaaatcttc actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg
    57541 ttccggcctg tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc
    57601 catttacaat ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga
    57661 gattcaggcc tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct
    57721 tatcgaataa cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc
    57781 tggagatttc cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg
    57841 atatggacgg cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc
    57901 ctgaaacatg gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc
    57961 agcaatatca cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc
    58021 aactgggtct ggatatctgt gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg
    58081 aagataccat tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca
    58141 ccaatgctca tccgcacaac ctggcggtaa aacttgagca tacgggtctg gacgcacacc
    58201 ttgatttatt actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc
    58261 atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg
    58321 aagcgattct cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc
    58381 ctgattccgg gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc
    58441 gcctgatccc ctcgctaatg tgaaggagac gccatgaaag agaaacctgc tgttgaggtt
    58501 cgactggata aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa
    58561 atgattaaag gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc
    58621 gagctgaatg ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag
    58681 gcgattactg aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg
    58741 gaaagtgtag agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg
    58801 ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac
    58861 ggcgacagtg aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta
    58921 catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc
    58981 ctgcaacaga tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa
    59041 ctgctggttg cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta
    59101 cagctgcagg gcgatggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa
    59161 atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg
    59221 gtcggcaatg gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc
    59281 gtagttggtc tggaaggtga taccctggcg gcctgcctag aagattactt tatgcgttct
    59341 gaacagctgc cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca
    59401 ggcggtatgt tgttgcaggt aatgcctgcg caaaataccc agcaggacga ctttgaccac
    59461 ctggcgacgc taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa
    59521 gtgttgtggc gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag
    59581 ttcaaatgca cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa
    59641 gaagttgata gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt
    59701 aaccactatc tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca
    59761 gatccgcaag ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgact
    59821 taatggcctg atgtgctgcg cttaccagcg cctatgaaat aaatcgcaaa ttattttggt
    59881 cagatacagc gtcgtatccg ataaaagcgt tgtcacttac ttaagtttat ttccagcgtt
    59941 tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc
    60001 gcagctgtga gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg
    60061 tcccattctt ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca
    60121 aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag tacgcaggcg
    60181 tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc
    60241 gcatccggtt taacagaacc aggggcaatt aacacatttt cggctaatac tttttccgat
    60301 acctgttcct gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc
    60361 gggtctttca taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga
    60421 ccgctctgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa
    60481 cgcgccatat gactgttcac gctaatacgc atactgtcca gcaccggcga attaagaagc
    60541 accagtatca ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg
    60601 ggattttgcc cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac
    60661 accagctccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc
    60721 agccataacc cccaggtggc gacgaacaca tacagcggag caaccagcaa agcggtttta
    60781 atcaggcaac gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata
    60841 gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg
    60901 cgagaaatcg cgctcaggcc cgtaaacggc agggtgatga taaacatcag ggttagcaat
    60961 gatactaacg gcagcaaccc cgtggcgatt agcgtgaaca acttttgaat agagtcgatt
    61021 aaacgtgact gtgtacgcgc gaggatcacc gccagtgcgg taaccagacc aaatgtaaga
    61081 tacataaacc agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta
    61141 aacaactcac cccacagtaa aagcaccagc cacgtcaggc cattggagag aaaaatcacc
    61201 agtaatataa gtacgttatg ccatactgac tggtaaaagt agctatagcg gctactgcca
    61261 ttgcggatac gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga
    61321 tagcagccaa aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc
    61381 cacttcagcc aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa
    61441 aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc
    61501 gcaggcacgc cataaacaat ccaactgtga ttttttccgg caagccaggc gataagcagg
    61561 tagcaaacca gcccctgaag taatccggta gcaatcatcc cccagcgtgt tgccggtgaa
    61621 agttcaacgt tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg
    61681 ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat
    61741 actttaccgg ttgaatttgc atcaatttca ttcaggaatg cgattccact cacaatattc
    61801 ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt
    61861 ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt
    61921 tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact
    61981 attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc
    62041 cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct
    62101 acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt
    62161 taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag
    62221 ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca
    62281 aaggtaagaa cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg
    62341 tgaccaggca gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc
    62401 cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca
    62461 aaaacatgtt tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg
    62521 ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctg aactccgaaa
    62581 acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg
    62641 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
    62701 cttctatgca ctgctccgcc aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc
    62761 tttccggcac cggtaaaacc accctttcca ccgacccgaa acgccgcctg attggcgatg
    62821 acgaacacgg ctgggacgat gacggcgtgt ttaatttcga aggcggctgc tacgcaaaaa
    62881 ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgtgatgcgc
    62941 tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa
    63001 ccgagaacac ccgcgtttct tatccaatct atcacatcga taacatcgtt aagccggttt
    63061 ccaaagcggg ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc
    63121 cgccggtttc tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg
    63181 ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct
    63241 tcggcgcggc gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta
    63301 tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac
    63361 gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt tcactggata
    63421 atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg
    63481 tagacacgaa gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa
    63541 aagccgaaac cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg
    63601 cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc
    63661 tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct
    63721 tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc
    63781 ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc
    63841 acaattgcca gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac
    63901 ggctggaaca ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg
    63961 aaccaggcgc gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg
    64021 gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcact
    64081 tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca
    64141 atcacctcac cgagcactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg
    64201 cgcaggtagt cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc
    64261 gattctgcca gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc
    64321 agcggcgtgc gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt
    64381 tgcttaacac cagccgccat atggttaaag gcacgggtaa cggaacgcac ctccgaagcg
    64441 ccatattcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg
    64501 agatcgacca acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat
    64561 agcataatcg ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc
    64621 agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa
    64681 cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg
    64741 cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag
    64801 tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac
    64861 tgcgtgccgt cctccagttg cagtttatcg gtcatcaaca tacgcacttc gtacgcgagg
    64921 actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc
    64981 gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt
    65041 cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca
    65101 ggccccaaac ggtctgaatg taacgcggat gcgctggatc ttcttctacc atgcggcgca
    65161 gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa
    65221 ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta
    65281 ccgcaaactc accgctggtg agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac
    65341 cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg
    65401 gcagttcgtt cgcctgacga cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt
    65461 taaacggttt tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt
    65521 cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc
    65581 ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag
    65641 attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc
    65701 cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca
    65761 gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg
    65821 tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa
    65881 attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat
    65941 tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga
    66001 atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag
    66061 cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca
    66121 agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa
    66181 atcgaccgtc gcgtgcgcta tctcactaaa tgcctggaaa atctcaaaat tgtcgattac
    66241 tcccctcagc aggaaggcaa agtctttttt ggcgcgtggg tggagattga aaacgacgat
    66301 ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac
    66361 atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg
    66421 gtggtgaata cccctgccgg ggaagcgaac tggtatgtta atgctatcga gtacgtgaaa
    66481 ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat
    66541 aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc
    66601 tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc
    66661 ctgcttgatg aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc
    66721 ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg
    66781 gaagagagac gtcaggcgat cctcaaatcc atttccgagc aaggcaaact caccgatgat
    66841 ctggcgaagg ccatcaacgc caccctaagc aaaaccgaac tcgaagacct ctacctgccc
    66901 tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg
    66961 gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgccgctgc acaatatgtt
    67021 gatgccgata aaggcgtggc agataccaaa gccgcgctgg atggcgcgcg ctatatcctg
    67081 atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag
    67141 aacgcgcatt tggtttctac ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc
    67201 gactatttcg atcatcacga accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg
    67261 ttccgtgggc gtaacgaagg cgtactccag ctttcgctga atgccgatcc gcagttcgat
    67321 gagccgccca aagagagcta ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg
    67381 aacaatgccc cggcggatag ctggcgcaaa agcgtagtga gctggacctg gcgcatcaag
    67441 gtgctgatgc atctggaaac cgaactgatg ggtaccgtgc gcgaacgcgc ggaagatgaa
    67501 gcaatcaacg tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg
    67561 cgtgcaacga tgggcctcga tccgggtctg cgtaccgggg taaaagtggc ggtggtcgat
    67621 gccactggca aactggtggc gaccgatacc atttacccgc acaccggaca agccgcaaaa
    67681 gcagcgatga ccgttgctgc cttgtgtgaa aaacataacg ttgaactggt ggcgatcggc
    67741 aacggtacag cttcccgcga aactgaacgt ttctatctcg acgtgcagaa gcagtacccg
    67801 aaagtgaccg cacagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcagcttcc
    67861 gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc
    67921 gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc
    67981 gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact ggatgcagta
    68041 gtagaagact gcgtaaacgg cgttggcgtc gatctcaaca ccgcttctgt tccgctatta
    68101 acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag
    68161 aacggccagt tccagaaccg tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc
    68221 ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg ataacccgct ggacgcctct
    68281 accgttcatc cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca
    68341 ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact
    68401 gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt
    68461 cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac
    68521 gacctgcaac cgggtatgat cctcgaaggc gcagtgacca acgtcaccaa ctttggcgcg
    68581 tttgtcgata ttggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag
    68641 tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg
    68701 gaagtggatc ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga gcagcctggc
    68761 gaaaccaact ctcgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc
    68821 aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg
    68881 gcagcgatgg gtaaaaaacg ttaaacgccc ggactggccc ctaaacctgc gcgataaggc
    68941 gttcacgccg catctggcaa cgaacgcttt gcctgacata aaagtgccgg agaatatctc
    69001 cggcactttt attccacagc caaactcaca atatactccg ttaatattta tcacttcatt
    69061 aacaactgaa accttaatta aacattagcc agtccgggta actcactatt cgaattatat
    69121 tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa
    69181 cattcgtaca cattttaagt attgctgata gaaaccattc tcattatcat tgtgttgttg
    69241 attatttaat ctctcctttg ttggcaaatc atctggtctc atgtcgcggt caaacgcccc
    69301 atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata
    69361 ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc
    69421 tttctctagg catgttacct ggctcctctt ttgatgtggt gcgcgtcgct ccactcggcg
    69481 accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct
    69541 tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc
    69601 attggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc
    69661 gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc
    69721 tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc
    69781 atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac
    69841 gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg
    69901 ctgcaactgc tggaactcgg cattccctgc atcgtggcgc tgaacatgct cgacattgcc
    69961 gagaagcaaa atattcgtat tgaaattgat gctctatcgg cacgtcttgg ctgcccggtg
    70021 atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc
    70081 tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca
    70141 gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc
    70201 ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat
    70261 ctggatgcca ccctcgcccg tctgcgtaat gaaatggatg atccggcgct gcacattgcc
    70321 gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca
    70381 gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg
    70441 ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg
    70501 ttacagccgc tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt
    70561 ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc
    70621 ggtattaaca ccgtgctgcc actggtgccg cagatcggca tgatgtacct gttcctctcc
    70681 ttccttgagg actccggcta tatggcgcgt gcagcgtttg tgatggaccg cctgatgcag
    70741 gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta
    70801 ccgtcggtaa tgggcgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg
    70861 atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc
    70921 ttcttcgggc agaacggtgc cctggcggtt ttctcgctgt atatgctcgg tatcgtgatg
    70981 gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc aacgccgttt
    71041 gtcatggagc tgccggtcta ccatgtacca cacgttaaaa gcctgattat ccagacctgg
    71101 cagcgtctga agggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt cagcattttc
    71161 ctgagcgctt tcaacagctt ctcgttgagc gggaaaatcg tcgataacat caacgactcg
    71221 gcgctggctt ccgtcagccg ggtgataacc cctgtcttca agccgattgg cgtacatgaa
    71281 gataactggc aggcaacggt tggcctgttt acgggcgcaa tggcaaaaga agtggtggtg
    71341 ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggcg
    71401 gaattcaacc tcggtgaaga gctgttcagt gcggtagatg aaacctggca gagcctgaaa
    71461 gacaccttca gccttagcgt actgatgaac cccattgaag ctagcaaagg cgacggtgaa
    71521 atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc tgccgcatac
    71581 agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg agccatcgcc
    71641 cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac
    71701 tcactggcaa cattgttcta tcaggtcgcc agctacagtc agcacccaac ttacagcctg
    71761 gtgtgcattc tggcggtaat cctgtttaac atcgtagtta tcggtctgct gcgccgcgcg
    71821 cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca
    71881 gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtacgt gatttgctgg
    71941 cgttacggtg ccgtatggaa gcgacccaga taagccagac attgaacact ccgcagccaa
    72001 tgattaacgc catgctgcaa cagctggaaa gcatgggcaa agccgtgcgg attcaggaag
    72061 aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggcaaa gcctgtctgc
    72121 gcgagtggtg ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag cccggtatgc
    72181 gtttgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaattct tgcagcgcaa
    72241 cgtaagttcc tctgaaagcg tctcgcaggg atgaaaactc gctaatgcac agatgtggag
    72301 tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag taaaaagcag
    72361 agttccaccc cacatgatgc gctgttcaaa ctctttttac gccaaccgga gacggcacgc
    72421 gattttcttg cgtttcattt accggcaccc attcacgcgc tctgtgatat gaaaaccctc
    72481 aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc cgatgtgctg
    72541 tggtcggtga aaacggaaca aggaccagga tacatctatt gtctgattga acatcaaagc
    72601 acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat
    72661 caccttgatg ctggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt
    72721 attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaaactg
    72781 gcaaggcagc tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat
    72841 gaaatcatgc agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa
    72901 cgcgatctga tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat
    72961 gacagacaaa tcaaagggct gtttaattac atactgcaaa ctggcgacgc tgtacgtttt
    73021 aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag tttaatgact
    73081 attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat agccaaaata
    73141 atgcttgaat ccggagtccc tcttgcagac atcatgcgat ttaccgggct gtcagaagaa
    73201 gagttggctg cggcgagtca gtagatactc taccctgctt aagtgctact ttgtgctatc
    73261 ttgcatgtaa cacaaagtag cacttaggga actgatatgc agacagtgaa attacggcaa
    73321 caaggaggag ccatgatcgt tactatccca cgagatcttg ccatcgatct tggctggtca
    73381 ccgggcacag agcttaccgt ggagaaaaaa ggcgatagcg ttaatttgcg cgccacagag
    73441 cacaaaccgc gtgggcgtct taccgtcgcg cagttgttga gccaaatcga tgaaagcgaa
    73501 attacggagc taaatcagtc cacagaagga tgggctgaag ggaaaaaagg taatgaagca
    73561 tggtaaaggc acggacgcca catcgtggtg agatctggta ttttaaccct gatccggttg
    73621 ccgggcatga acttcagggg ccacattatt gcattgtggt aacggacaaa aaactcaaca
    73681 atgttttaaa agttgctatg tgctgcccga tttcaacagg ggcaaatgca gcacgttcca
    73741 caggggtgac ggtgaacgtc ctcccctgtg atacgcaaac cggtaacctg catggcgttg
    73801 tactttgtca ccagctaaaa gccgtcgatc ttattgcccg tggcgctaaa tttcataccg
    73861 ttgccgatga aaaattgatt agtgaagtta tcagtaaact ggtgaattta atcgacccac
    73921 aataattagc tctcacttac caccaacacc ctctgcttca acgccaccag caggtgacaa
    73981 aactcgaccg gatgcgaaat aaacggcgca tgggccgctt tggcgaagat atatgattcg
    74041 ctgtgaggcc aaagtttatc cagcatcggc accactttgc gcggcaccag accgtcgaga
    74101 tagccataca atcgcaaaaa cggcatggac acgttttgca gcggctgacg gagatcgacc
    74161 gttttcagga tttccagccc gccattaagc acgtcaacct ccggcatcgg taacgccaga
    74221 acggttttct tcaacgcccg cgcatcctgg cgcgccgttt cagtccccat ggtttgtaac
    74281 gccaggaacc gctccactgt acgctgaaaa tcatcactga gttgctgctg aaatcccgcc
    74341 agcacgtccg gttttatccc cggccactcg tcacgagcac taaaataagg tgacgacgcc
    74401 acggtgacca gcgcctgaac acgctcggga tgggttaacg caatctggct tgccaccagc
    74461 ccgcccagac tccagcctaa ccaaatggct ttatcaggtg cctgttgcag cacggcttcg
    74521 gccatatcag caagtgacag cgcaccaaat ccccggctac gcccgaagcc gggcaggtca
    74581 acaaggtgca gcgtaaaatg cgagctaagt tcctcgtcaa tgcaacgcca cacttcggca
    74641 ttcagtcccc atccgtgcag cagcacaaga tgaacattcc cctgaccttt ggtctgccac
    74701 cagatgttat tcatccgcta ttgttctctt ttgacttaca aggatgaaca tatgctaaca
    74761 gtaccgggat tatgctggct atgccgaatg ccactggcgt taggtcattg ggggatttgt
    74821 tcggtctgtt cacgcgccac ccgcacagat aaaacgttat gcccacaatg tggattaccc
    74881 gccacacact cccatcttcc ctgcggtcgc tgcctgcaaa aaccgccgcc ctggcaaaga
    74941 ctggtcacgg ttgccgacta tgcgccgccg ttaagtccgc ttatccacca gcttaaattt
    75001 tcccggcgca gtgaaatcgc cagcgccctg tcacgtctgt tactattgga agtcttacac
    75061 gctcgtcgca ccaccgggtt gcaattgccg gatcgcatca tcagcgttcc gttatggcag
    75121 cggcgtcact ggcgtcgggg atttaatcag agcgatttgc tgtgtcagcc gttatcacgc
    75181 tggttgcact gccaatggga tagcgaagcc gtcacacgta cacgggccac tgcgacccag
    75241 cattttctca gtgcccggct gcgcaagcgc aacctgaaaa atgcctttcg tcttgaattg
    75301 cccgtgcaag gtcgccatat ggtgattgtg gatgatgtcg ttaccaccgg aagtaccgtc
    75361 gcagagattg cgcagttgct tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt
    75421 tgtcgaacct tgtagagcct cgatgatggg cgtattataa ccaactaaaa tagtcaacta
    75481 ttaggccatt actatgatcc gtatttccga tgctgcacaa gcgcactttg ccaaactgct
    75541 ggcaaatcag gaagaaggga cacaaatccg cgtatttgtg attaaccctg gcacgcctaa
    75601 cgctgaatgt ggtgtttctt attgtccgcc ggatgccgtg gaagccaccg acactgccct
    75661 gaaatttgac ctgctgaccg cgtatgttga tgagttaagc gcaccatacc tggaagatgc
    75721 agagatcgac tttgttaccg accagttggg ttctcagtta acgctgaaag ccccgaacgc
    75781 caaaatgcgt aaagtggcag acgatgcacc gctgatggag cgcgtggagt atatgctgca
    75841 gtcgcagatc aacccacagc ttgctggtca cggtggtcgc gtttcgctga tggaaatcac
    75901 cgaagacggt tacgccattc tgcaatttgg cggcggctgt aacggttgtt ccatggtcga
    75961 tgtgacgctg aaagaaggga tcgagaagca gctgctgaac gaattcccgg agctgaaagg
    76021 tgtgcgcgat ctcaccgaac accagcgcgg cgaacactcc tactactaag ttatcccctc
    76081 ttttgtagat tgcccgatgc gacgctaaag cgtcatttcg ggcctacgtt gaacatgtgc
    76141 cagacgttgg cacagcattg ctttggcaaa tccctccctt cgtttccccg cgataatatg
    76201 accaacctct cataatttaa atttaccccg ctctggtgat tctcaaacgc cagatgttac
    76261 ccgtatcatt cacatgggta ccaaacatac tcctgacatc tgactacaat aattagtttt
    76321 agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg attgggataa ttagagtttg
    76381 tcgtcagaaa attgacgtta cccataacaa atgaaaggcc aggtaaatca tgccattagt
    76441 cattgttgct atcggtgtaa tcttgttgtt gctcctgatg atccgcttca aaatgaacgg
    76501 cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta atgcaaggaa tgccgctgga
    76561 taaagttatt ggctccatca aagccggtgt cggcgggacg ctcggtagcc ttgccctgat
    76621 catgggtttt ggcgcaatgc tggcaaactg cggtggcgca caacgtatcg ccaccacgct
    76681 gattgccaaa tttggtaaaa agcacatcca gtgggcggtg gtgttaaccg gttttaccgt
    76741 tggttttgcc ctgttctatg aagtgggctt cgtgctgatg ctgccgctgg tgtttaccat
    76801 cgcggcttcc gcgaatattc cattgctgta tgttggtgta ccaatggcgg ctgcactgtc
    76861 tgtgacccac ggcttcctgc caccgcatcc gggcccgact gcgattgcca ccattttcaa
    76921 tgccgatatg ggtaaaaccc tgctgtacgg tactattctg gcaatcccga ccgtgattct
    76981 cgccggtccg gtttacgctc gcgtgttgaa aggtatcgat aagccaatcc cggaaggtct
    77041 ctacagcgcg aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac
    77101 ctctctggtg ccggttgtgc tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa
    77161 aggtcacgct ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg caacgctgat
    77221 tgccgtgctg attgcgatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat
    77281 taacgacacg ctggtttctt ccatcaaaat cattgcgatg atgctgttga tcatcggtgg
    77341 tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat
    77401 gatgcacgaa accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg
    77461 tatcgcgctg ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct
    77521 gattgcaacg acgggtgtta gcccagagct gatggttatt gcggtgggtt ccggtagtgt
    77581 gattttctct catgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac
    77641 tatcggcgag accatcaaat cctggtcgat gctggaaacc attatctcgg tgtgcggtct
    77701 ggtaggctgt ctgctgctga atatggtgat ttgataacac tgactgccgg atgcatcgca
    77761 tccgacatca accgcactct acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc
    77821 cttcagcaac ttgttcacgc catcatcggc aaacatcgac tcaagcgttg cggaaagctt
    77881 gcgtcgccag tttttatact ggtaactggt gccaggaata ttcaccggtt cggccatatc
    77941 cagccagtct tccggctgta gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg
    78001 caaaccacgg ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt
    78061 tggcagacaa ccatatttat gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc
    78121 ctgatacaga ccgcgcagta ccacttcatc cggatacagc cccagggttt tgcccagcgt
    78181 tagatccccg ctctcccagt aaccacgcag cgttggcagg tcatgtgtcg ccgcaaccgc
    78241 catcgactgc tccggatacg ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa
    78301 atagagcact ttgtaagagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg
    78361 tacggtaccg agatcttcac caatcaccat acagcgatga cgtttacttt cgagtgccag
    78421 aatcgagagc agatcatcca ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc
    78481 atacggtatc caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca
    78541 gttttgcata ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgatgat
    78601 atgcgggtcc attggcggta atccccagtt ctgccccaac gggccgagga tatccggcgg
    78661 cgcgccaacc gatgctttca ggcaatatag ttcacggtca caccaggttt ccgccccacc
    78721 ttccgctacg ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct
    78781 tatctcccag caggcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc
    78841 gacgtcatca cgatgttctt cgcagaactg acgcacttct ggtgaatcca cgttcttata
    78901 catctctggc catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg
    78961 tagcgcatca aaggctgcct gccagaacag gctgtcgccc tgctctgcaa caaactggcg
    79021 aaacgcggcc atctgctcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc
    79081 tgtcattttt agggcggtaa ccgtggagta atcgacccag tcggcatcgc gcgcctgttg
    79141 cagcgtctgt tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc taagatggaa
    79201 atcttcaacg gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct
    79261 gtatgggctg gcgctctccg gatttgccgg atagagcgca tgaatcgggt tcaggccaat
    79321 gaacgacccg ccacgttttg ccacatccac cagcatcgct ttgagatcgc caaaatcccc
    79381 aataccccag tttttttccg atcgcagcgt ataaagctga acgcaggcac cccacagctt
    79441 ttgtttattc agcaaagcct gcggttcgta acaacgtttc ggggcgacaa tcacccggca
    79501 atgcgcgcgc tggtcgtcct gggtgagtgt tagcgtgtga taaccttccg gcagcttcgt
    79561 cggtagattg aacgctttgc cccccgttac atggcctttg tactgcgttc cttcttcggt
    79621 ggtcagctgc cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct
    79681 ggtataaacc atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg
    79741 catcgcgtca agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc
    79801 attgatgtaa ttggggctaa tccccgccgc cagcgcggca ttatccagac gtttgctttc
    79861 catcgagctt ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg
    79921 atccgagcta aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc
    79981 ctggtcgcgg tacagcacat ccacctgctt ttgtgcctca acataggctg cgaaatccgc
    80041 catcaccaga tacggatcgc cgccctgttt gccgatactg tgcagcatct ggtcgaaggc
    80101 atgcttatcg ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac
    80161 cttatctttc ttccgccatt tcaccgggtc gtagcctttg gccagaatag ccttcacttg
    80221 ttccacggta tgaccaaaaa taaagatatt ttcttcaccg actttctcgg cgatttcaac
    80281 gttcgcacca tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc
    80341 ggtaccggaa gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat
    80401 cagtttttcc gccgccgaaa cgcaataatc cggcaggaac accaccttca acttatcgcc
    80461 aaccagtgga tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattctt
    80521 cgccaggtag tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc
    80581 agcctgcagg ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg
    80641 ctggcgtttg tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat
    80701 ctcaataccg gtacgaactt tcacaaactc cgccagacgg actttattcg cctgcttgat
    80761 ctcgcgatat tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg
    80821 atcgagatcg ttagcccact ctttttgcag tgatttatcc aacagagccg ccagtgcagg
    80881 gttgcactgt ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt
    80941 cggccatagc tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc
    81001 cgcaacaccg ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt
    81061 atcgtgcacc accgccagtt tggcccacac tttttcatcg cccggccagg ttttctccac
    81121 cagcgtttta aagcgagtat taatttcgtt aataatctgc atatggcgcg gcagtaagcc
    81181 tttcaccagt ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta
    81241 agcgaaagtt ttgctggtga tggcccaggc gtcatcccag ctcatctggt gctcatcggt
    81301 cagcacgcgc agcagttctg gaatcgcgat agttggatgg gtatcgttca gctgaataac
    81361 ttcgtaatcc gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc
    81421 cgctaccgaa caggcacact ggaagtattg ctgcatcagg cgcagctttt taccggcagt
    81481 atggttgtcg tttggataga gaactttggt cagtttttcc gcattgatgc cttgctgttc
    81541 ggcgcgcagg aaatcaccgt cgttaaattt agttagatca aacggatgcg catgcgtcgc
    81601 ctgccacaga cgcagcggct gcgccacgcc attacgatag ccgacaacgg ggagatccca
    81661 cgcttgaccg gtaatggtaa actccggctc ccagcgtccg tctttcatca ctttaccgcc
    81721 aatccctacc tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg
    81781 ccagtcatcc ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga acaaaccata
    81841 ttgatagttc agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa
    81901 gcacgccgcc agacgtccca gaccaccgtt acccagcgcc gggtcgatct cttcttccag
    81961 caggtcagtc aggttgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc
    82021 gagattcaac aggttgttgc ccgtcagacg accaatcaaa aactccattg agatgtagtt
    82081 aacatgtcgc tgattcgcca ccggtttggc gaatggctga gcacgcagca tttcggccag
    82141 tgcttcactc actgccagcc accactggcg aggagtcatt tcagccgcag aatttaagcc
    82201 ataacgctgc cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg
    82261 tgacatagga gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg
    82321 ctcgatgttg accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag
    82381 caaggaaatg tgatctcgac cacttaaagc tagtgcaaac cacaggatta gcagcaaatc
    82441 aatgccatac agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc
    82501 aaaaagcgga aggtaactct ataaattaag taaaggagtg aaacagtttc acaagtaaaa
    82561 tatccagtgt gctccatctc attcttaata gatttattaa gatcatcttt ttagatggca
    82621 ctttcatcag gaatgaagaa gaaacccttg cttaaatgaa tctgat
//
LOCUS       DADWTH010000003        76977 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.003, whole genome shotgun sequence.
ACCESSION   DADWTH010000003 DADWTH010000000
VERSION     DADWTH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.003"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="JNY02_000177"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="JNY02_000177"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBB9431863.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JNY02_000178"
     CDS             complement(1672..2730)
                     /locus_tag="JNY02_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB9431864.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JNY02_000179"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JNY02_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JNY02_000180"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JNY02_000180"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBB9431865.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNY02_000181"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNY02_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBB9431866.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNY02_000182"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNY02_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBB9431867.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JNY02_000183"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JNY02_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBB9431868.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JNY02_000184"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JNY02_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBB9431869.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLANPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRHRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="JNY02_000185"
                     /pseudo
     CDS             complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="JNY02_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="JNY02_000186"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="JNY02_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBB9431870.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="JNY02_000187"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="JNY02_000187"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBB9431871.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRNILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="JNY02_000188"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="JNY02_000188"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBB9431872.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="JNY02_000189"
     CDS             complement(17698..18321)
                     /locus_tag="JNY02_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBB9431873.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /locus_tag="JNY02_000190"
     CDS             18618..18893
                     /locus_tag="JNY02_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431874.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="JNY02_000191"
     CDS             complement(18970..19212)
                     /locus_tag="JNY02_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBB9431875.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="JNY02_000192"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="JNY02_000192"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBB9431876.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="JNY02_000193"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="JNY02_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBB9431877.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="JNY02_000194"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="JNY02_000194"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBB9431878.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22591..24288
                     /gene="ypdA"
                     /locus_tag="JNY02_000195"
     CDS             22591..24288
                     /gene="ypdA"
                     /locus_tag="JNY02_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBB9431879.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24303..25037
                     /gene="ypdB"
                     /locus_tag="JNY02_000196"
     CDS             24303..25037
                     /gene="ypdB"
                     /locus_tag="JNY02_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBB9431880.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25050..25907
                     /locus_tag="JNY02_000197"
     CDS             25050..25907
                     /locus_tag="JNY02_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9431881.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="JNY02_000198"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="JNY02_000198"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBB9431882.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="JNY02_000199"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="JNY02_000199"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBB9431883.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="JNY02_000200"
                     /pseudo
     CDS             complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="JNY02_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="JNY02_000201"
     CDS             complement(30571..31818)
                     /locus_tag="JNY02_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBB9431884.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGENGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="JNY02_000202"
     CDS             complement(31840..32166)
                     /locus_tag="JNY02_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBB9431885.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="JNY02_000203"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="JNY02_000203"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBB9431886.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="JNY02_000204"
     CDS             33554..34810
                     /locus_tag="JNY02_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBB9431887.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /locus_tag="JNY02_000205"
     CDS             34925..35251
                     /locus_tag="JNY02_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBB9431888.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35298..36536)
                     /locus_tag="JNY02_000206"
     CDS             complement(35298..36536)
                     /locus_tag="JNY02_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBB9431889.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36872..38074
                     /gene="nupC"
                     /locus_tag="JNY02_000207"
     CDS             36872..38074
                     /gene="nupC"
                     /locus_tag="JNY02_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBB9431890.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38124..40313)
                     /locus_tag="JNY02_000208"
                     /pseudo
     CDS             complement(38124..40313)
                     /locus_tag="JNY02_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40521..40596)
                     /locus_tag="JNY02_000209"
     tRNA            complement(40521..40596)
                     /locus_tag="JNY02_000209"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40561..40563),aa:Ala,seq:ggc)
     gene            complement(40636..40711)
                     /locus_tag="JNY02_000210"
     tRNA            complement(40636..40711)
                     /locus_tag="JNY02_000210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40676..40678),aa:Ala,seq:ggc)
     gene            40932..41291
                     /locus_tag="JNY02_000211"
     CDS             40932..41291
                     /locus_tag="JNY02_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBB9431891.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41293..41685
                     /locus_tag="JNY02_000212"
     CDS             41293..41685
                     /locus_tag="JNY02_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBB9431892.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41737..43152)
                     /gene="gltX"
                     /locus_tag="JNY02_000213"
     CDS             complement(41737..43152)
                     /gene="gltX"
                     /locus_tag="JNY02_000213"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBB9431893.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43411..43486
                     /locus_tag="JNY02_000214"
     tRNA            43411..43486
                     /locus_tag="JNY02_000214"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43444..43446,aa:Val,seq:tac)
     gene            43491..43566
                     /locus_tag="JNY02_000215"
     tRNA            43491..43566
                     /locus_tag="JNY02_000215"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43524..43526,aa:Lys,seq:ttt)
     gene            43597..43722
                     /locus_tag="JNY02_000216"
     ncRNA           43597..43722
                     /ncRNA_class="other"
                     /locus_tag="JNY02_000216"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43842..44183
                     /locus_tag="JNY02_000217"
     CDS             43842..44183
                     /locus_tag="JNY02_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBB9431894.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44174..45100)
                     /locus_tag="JNY02_000218"
     CDS             complement(44174..45100)
                     /locus_tag="JNY02_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9431895.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45190..46188
                     /locus_tag="JNY02_000219"
     CDS             45190..46188
                     /locus_tag="JNY02_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBB9431896.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46185..46403)
                     /locus_tag="JNY02_000220"
     CDS             complement(46185..46403)
                     /locus_tag="JNY02_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBB9431897.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46405..48420)
                     /gene="ligA"
                     /locus_tag="JNY02_000221"
     CDS             complement(46405..48420)
                     /gene="ligA"
                     /locus_tag="JNY02_000221"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBB9431898.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGSTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQELMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48491..49378)
                     /gene="zipA"
                     /locus_tag="JNY02_000222"
     CDS             complement(48491..49378)
                     /gene="zipA"
                     /locus_tag="JNY02_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBB9431899.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMT
                     PQKLREYQDIIREVKDANA"
     gene            49608..50369
                     /gene="cysZ"
                     /locus_tag="JNY02_000223"
     CDS             49608..50369
                     /gene="cysZ"
                     /locus_tag="JNY02_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBB9431900.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50554..51525
                     /gene="cysK"
                     /locus_tag="JNY02_000224"
     CDS             50554..51525
                     /gene="cysK"
                     /locus_tag="JNY02_000224"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBB9431901.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51909..52166
                     /gene="ptsH"
                     /locus_tag="JNY02_000225"
     CDS             51909..52166
                     /gene="ptsH"
                     /locus_tag="JNY02_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBB9431902.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52211..53938
                     /gene="ptsI"
                     /locus_tag="JNY02_000226"
     CDS             52211..53938
                     /gene="ptsI"
                     /locus_tag="JNY02_000226"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBB9431903.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53979..54488
                     /gene="crr"
                     /locus_tag="JNY02_000227"
     CDS             53979..54488
                     /gene="crr"
                     /locus_tag="JNY02_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB9431904.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54530..55381)
                     /gene="pdxK"
                     /locus_tag="JNY02_000228"
                     /gene_synonym="thiJ"
     CDS             complement(54530..55381)
                     /gene="pdxK"
                     /locus_tag="JNY02_000228"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBB9431905.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55486..55860
                     /locus_tag="JNY02_000229"
     CDS             55486..55860
                     /locus_tag="JNY02_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBB9431906.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55893..56616
                     /locus_tag="JNY02_000230"
                     /pseudo
     CDS             55893..56616
                     /locus_tag="JNY02_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56820..57731)
                     /gene="cysM"
                     /locus_tag="JNY02_000231"
     CDS             complement(56820..57731)
                     /gene="cysM"
                     /locus_tag="JNY02_000231"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBB9431907.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57865..58962)
                     /gene="cysA"
                     /locus_tag="JNY02_000232"
     CDS             complement(57865..58962)
                     /gene="cysA"
                     /locus_tag="JNY02_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBB9431908.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58952..59827)
                     /gene="cysW"
                     /locus_tag="JNY02_000233"
     CDS             complement(58952..59827)
                     /gene="cysW"
                     /locus_tag="JNY02_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBB9431909.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59827..60660)
                     /gene="cysT"
                     /locus_tag="JNY02_000234"
     CDS             complement(59827..60660)
                     /gene="cysT"
                     /locus_tag="JNY02_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBB9431910.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60660..61676)
                     /gene="cysP"
                     /locus_tag="JNY02_000235"
     CDS             complement(60660..61676)
                     /gene="cysP"
                     /locus_tag="JNY02_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBB9431911.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61980..62771)
                     /gene="ucpA"
                     /locus_tag="JNY02_000236"
     CDS             complement(61980..62771)
                     /gene="ucpA"
                     /locus_tag="JNY02_000236"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBB9431912.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62958..63758)
                     /gene="murR"
                     /locus_tag="JNY02_000237"
     CDS             complement(62958..63758)
                     /gene="murR"
                     /locus_tag="JNY02_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBB9431913.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63922..64818
                     /gene="murQ"
                     /locus_tag="JNY02_000238"
     CDS             63922..64818
                     /gene="murQ"
                     /locus_tag="JNY02_000238"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBB9431914.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64822..66246
                     /gene="murP"
                     /locus_tag="JNY02_000239"
     CDS             64822..66246
                     /gene="murP"
                     /locus_tag="JNY02_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBB9431915.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66426..67325)
                     /gene="yfeX"
                     /locus_tag="JNY02_000240"
     CDS             complement(66426..67325)
                     /gene="yfeX"
                     /locus_tag="JNY02_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBB9431916.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67421..67996)
                     /locus_tag="JNY02_000241"
     CDS             complement(67421..67996)
                     /locus_tag="JNY02_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBB9431917.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68057..68506)
                     /locus_tag="JNY02_000242"
     CDS             complement(68057..68506)
                     /locus_tag="JNY02_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBB9431918.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68493..68918)
                     /locus_tag="JNY02_000243"
     CDS             complement(68493..68918)
                     /locus_tag="JNY02_000243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBB9431919.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69132..70001
                     /gene="amiA"
                     /locus_tag="JNY02_000244"
     CDS             69132..70001
                     /gene="amiA"
                     /locus_tag="JNY02_000244"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBB9431920.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70005..70904
                     /gene="hemF"
                     /locus_tag="JNY02_000245"
     CDS             70005..70904
                     /gene="hemF"
                     /locus_tag="JNY02_000245"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBB9431921.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70910..71963)
                     /gene="eutR"
                     /locus_tag="JNY02_000246"
                     /pseudo
     CDS             complement(70910..71963)
                     /gene="eutR"
                     /locus_tag="JNY02_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72048..72509)
                     /gene="eutK"
                     /locus_tag="JNY02_000247"
     CDS             complement(72048..72509)
                     /gene="eutK"
                     /locus_tag="JNY02_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBB9431922.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72522..73181)
                     /gene="eutL"
                     /locus_tag="JNY02_000248"
     CDS             complement(72522..73181)
                     /gene="eutL"
                     /locus_tag="JNY02_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBB9431923.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73191..74078)
                     /gene="eutC"
                     /locus_tag="JNY02_000249"
     CDS             complement(73191..74078)
                     /gene="eutC"
                     /locus_tag="JNY02_000249"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBB9431924.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(74099..75460)
                     /gene="eutB"
                     /locus_tag="JNY02_000250"
     CDS             complement(74099..75460)
                     /gene="eutB"
                     /locus_tag="JNY02_000250"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HBB9431925.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTSTVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75649..76854
                     /locus_tag="JNY02_000251"
     CDS             75649..76854
                     /locus_tag="JNY02_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBB9431926.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWVNLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
BASE COUNT        19568 a        19922 c        18494 g        18993 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaagctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggcaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcatcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgattctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc taggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgctgca gggtcgataa ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggtagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccctca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatattgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata atgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagatac cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc cgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gctacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgcc
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgtgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgaga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcttatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggtgtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccaaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagc
    22441 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    23041 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    23101 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    23161 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    23221 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    23281 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    23341 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    23401 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    23461 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    23521 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    23581 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    23641 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    23701 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    23761 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    23821 gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag
    23881 caggcccgct ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc
    23941 tgcattccca gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag
    24001 ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc
    24061 attgcggtgc gagataccgg gcacggcatc gatccaaagg tgattgagcg ggtcgaagcg
    24121 aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg
    24181 tatggcgagg ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt
    24241 cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata
    24301 ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa
    24361 ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga
    24421 agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg
    24481 atggcgtgtt gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca
    24541 tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc
    24601 tcaaaccgta tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc
    24661 aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taaaatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg caccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccatt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgta tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaagattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acctgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca gatattcagc
    35341 gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct gttcaccaga
    35401 tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc cagggcgata
    35461 ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa tcccatcaga
    35521 atgacaataa atgacggcaa catggtgact gtacgacgca cccacagcgg gatatggaag
    35581 cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt tgaggacagt
    35641 ccggcagcaa ccagacttaa cccaaagacc gttgccgcag catggcttaa cagcggttgc
    35701 agcgtcagat aagcctcatc aagatcggca acaccagtat gaccagaaaa gtggaacgcc
    35761 gccgcagctg tagccatcat cgccagattg acaaaaccgg caatcgtcat ggcgatagcc
    35821 acatcccatt tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg
    35881 agcgaagagt gcaaataaat cacatgcggc ataatcgtcg cccctaacac gcctgctgcc
    35941 aggaagaccg cctccgaagt aggtaaactc gggatcacca ttcctttacc cagctgcgcc
    36001 aggttaggct gggagaaaat caactcgaca atgtaagccg cggcaacaaa caacagtaac
    36061 ccgccaatca ctttctccag cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa
    36121 gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa
    36181 ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaataat ttctgcctga
    36241 acccaataga accacactac gggacgcgga tagtgatcac gaatctgctc cgccagattt
    36301 ttaccggtgg caatccctag tttggcagag aggatctgaa ttagcatcgc catcaggttg
    36361 gcccaaacga caacccacag tagctgatag ccgaagctag cacccgcctg aatattggtc
    36421 gcaaagttac cgggatcgat ataaccaatc gccgcaatga acgcaggtcc cattaatgcg
    36481 agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg
    36541 tgcctctaaa acatagcctt tgctatgttt catgctatgc taaacgagaa tgattatcaa
    36601 attcatttaa atggattgtg gtgatttctc tgatagacca agattatgat tacgaaaaga
    36661 ttgatgactt gaatgttatg gttatgttta atgttagcac atttacataa ctttcagctt
    36721 ccatacacaa catagcagaa atgtatgaca gatcactatt tttgaagcct gtcacaggac
    36781 gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga atgtgcacgg
    36841 aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt ttgtactggc
    36901 acttgccgtt gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat
    36961 ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct tcctgaactc
    37021 cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac tactcggatt
    37081 tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc tggcattctt
    37141 cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca ctgatcggta ttctccagca
    37201 cattcgcgtg ttgccggtga ttatccgcgc aattggtttc ctgctctcca aagtcaacgg
    37261 tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc agtcagaaaa
    37321 ctttattgcc tataaagata tcctcggcaa aatctcccgt aatcgtatgt acaccatggc
    37381 tgccacggca atgtccaccg tgtcgatgtc catcgttggt gcatacatga ccatgctgga
    37441 accgaaatac gtcgttgctg cgctggtact gaacatgttc agcaccttta tcgtgctgtc
    37501 gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac atccagatgt ccaacctgca
    37561 cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc
    37621 cattatcgtt gccgcgatgc tgatcggctt tatcgccctg atcgccgcgc tgaacgcact
    37681 gtttgccacc gttactggct ggtttggcta cagcatctcc ttccagggca tcctgggcta
    37741 catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac tgcaagtggg
    37801 cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa
    37861 aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc tggtttcctt
    37921 cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt aaaggcctga atgaagagca
    37981 aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc tggtgagtgt
    38041 gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat acataaaaaa gccggggatc
    38101 attccccggc ttttttatgc ctgttaatca gctaatggct gcgggcgacc aatcaaatac
    38161 ccttgctgat attgcacccc gagcttatgc aatagcgcct gctgctgttg cgtctcgaca
    38221 aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga tctcacaatc
    38281 atcgcatcca gcgtattcgt gacaatatct ttcacaaaga cgccatcaat tttgatgata
    38341 tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggcgccaaa gtcatcaatc
    38401 gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct ttctgcgtta
    38461 gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat gtgataacgc
    38521 ttaaacagac gaataatccg cccggcaata ttcttttgca gcagcgtgag cggcattaaa
    38581 ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca cttcaacaag
    38641 gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga
    38701 aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat ctcatcataa
    38761 ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc ctgatctaac
    38821 gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga aaccatctcc
    38881 tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc cagccagctt
    38941 aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc ccaggcagca
    39001 ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt caggatgtta
    39061 accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag cagttcacta
    39121 cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt
    39181 gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa ctcaagatta
    39241 tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    39301 cgaaagttgg gtaggagcgt taacggatct gttagcgcct gcaaatgcca gcggcgatta
    39361 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    39421 agcaccgcga gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
    39481 ttgtaattca gaaggcaaag cgttgaaaca gcctaggtga gatttaaaaa cggatagcga
    39541 agattaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    39601 aagtcgtttt cataaggtgt gcacaacaga agtaggagca cgcttaacgc tgccagccag
    39661 cttaaggtaa atgcgcgttt ctctttgccc aacgacggag cgatatccct gcgccacaat
    39721 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
    39781 atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg
    39841 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    39901 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    39961 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    40021 aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag
    40081 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    40141 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    40201 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    40261 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    40321 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    40381 caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct
    40441 aacccggacg taaactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt
    40501 tgcagcgagg gttggaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
    40561 gccatgcaag cgctctccca gctgagctat agccccacga tgcgtttacg taccaaattt
    40621 gctgggtgca aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
    40681 gcaagcgctc tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc
    40741 ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc
    40801 gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat
    40861 gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc
    40921 agggaattgt tatgtccaag gagaggatga caccagatga acttgccaga ctgaccggct
    40981 atagccgcca gaccattaat aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac
    41041 ctggcgtcca gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata
    41101 ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc
    41161 cgcttgaagt attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt
    41221 ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg
    41281 atagcaaata atatgaaaag attacgcaat aaaatgacca ccgaagaact ggctgaatgc
    41341 ctcggtgtgg ctaaacaaac tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa
    41401 aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc
    41461 gagtttattc agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag
    41521 agaattgctg aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg
    41581 atagataata tgacagacat tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga
    41641 attcgcaatt tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg
    41701 atattatctc ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc
    41761 aataaaatcc agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac
    41821 ggtaacatcc agtgctggag actgccccgc accggttacg gcgacacgca gcggcatacc
    41881 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
    41941 ttcagcggtc cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg
    42001 cgctaccgga cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta
    42061 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
    42121 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
    42181 ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
    42241 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta
    42301 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
    42361 cagatagttc agtagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
    42421 tgccccgtga cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc
    42481 gtaaaccggc accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt
    42541 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac
    42601 acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg
    42661 gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
    42721 gttagcaaaa cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca
    42781 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
    42841 gcgctcttta gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac
    42901 cgcgttgtag cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct
    42961 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
    43021 ggtgtcttca atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta
    43081 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
    43141 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
    43201 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc
    43261 taccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
    43321 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
    43381 ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc
    43441 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttc gggtcgttag
    43501 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
    43561 ccaccaatgt aaaaaagcgc cctaaaggcg cttttttact atctgcgata ctcaaagatt
    43621 cgaacctgca gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg
    43681 cccgaagggc gagcgaagcg agtcatcctg cacgacccac caatgcaaaa aagcgcccta
    43741 aaggcgcttt tttgctatct gcgatttgta agattgattt ttcttctcaa gttccttgtt
    43801 cttctaccga tattacctcg tacagtgacg aggactgaac aatggccact atctcttcaa
    43861 cttcaattcc ttccatacaa acacagagta gtaacagggc atcacaaggt agcgatgttg
    43921 cttcacaaat cgcgcggata agtcagcaaa tcatcaaact cactcaacaa attaaagaga
    43981 ttgtagacac tagcggcagt gctgaagaca agcaaaaaca ggccgaattg attcaacagc
    44041 aaatcaccct actggaaaca caactggcgc agttgcagaa acagcaggca gaaaaggcgc
    44101 aggagaaaga gcagcgtttg tctctgaatg tatcccttct aaatcctgtt gaaaaaacga
    44161 ctcatatcga tatctatatc tgatacaacg gatccccttc ccgaccagcc attagcgcat
    44221 tgccctggtc tcgtaccacg tcccataacg cttcagcggc ggtggaaagt gaacggttct
    44281 tcctgcgcac cagcatcaac tggcgttcaa ccaccggcgt aatgcgctta accaccagtg
    44341 ggctaccttc aggcaatggt agcgccagtg caggaagaat actgatgccg atgcctgcgg
    44401 ctaccatcgg gaacagcgtc gccggatgtc caatctcctg cacaatattc gcctgaatac
    44461 cattgcgcgc cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca
    44521 ccaatttcgc tccctgtaat gcctgccagg gtacataatc ttccacggcc aaagcgctat
    44581 cgcgatggca aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc agatcgccca
    44641 ctgggcctgg atcgatgacg atgccaaagt cgacatctcc ctggcgaata ctttccatca
    44701 cccactgctg cggacgatca tgcaggacaa actgaatatc tggatagcgg cgatggcttt
    44761 cagcaatgca ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt
    44821 ttccgctcag ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca
    44881 gcagtcgctc aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg
    44941 tggttctgtc cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactta
    45001 ctgccgactg gctcaagccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta
    45061 ctgtgacgaa aacctttaat tgttttaaag aataattcat ctgttttctt catgaatgga
    45121 tgcaataaat caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct
    45181 ggagtcttta tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg
    45241 gtgttgctgg cctctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg
    45301 accaccgcag ctattgccct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg
    45361 attattgctg gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg
    45421 ctgtttccga ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt cgacccgatg
    45481 ctctactccg gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc
    45541 ttcacgtcaa tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc
    45601 ctgctgggga ttttcctttc accattgctg gttggtctgg tgatgaacgt tcacggtgca
    45661 aggggcagcc ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg
    45721 gggcatcttt cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa atggattgcg
    45781 aaaactgacc agacgtccat tctgttggtg gtttatacag cgttcagcga agccgtcgtt
    45841 aatggtatct ggcataaagt tggctgggga tcattgctgt ttatcgtggt ggtcagctgc
    45901 gttcttctgg ctatcgtgat tgtagttaac gtctttatgg cacgccgact gggcttcaat
    45961 aaggcagatg aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc
    46021 ccgatggcaa acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg
    46081 attttccatc agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag
    46141 accgaacagt tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc
    46201 aggggctgga ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc
    46261 tcaccgagct tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc
    46321 ggcaggtcaa ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt attggctatt
    46381 tcaatcagct gctctttttc catctcagct acccagcaaa cgcagcattt ccgcttcgtc
    46441 gatgacttca atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc
    46501 tatcaccaga tcggttttct tcgacacgct gcccgcgact ttcgcgccca gttcgaccag
    46561 tcgagcttta gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt
    46621 accagcaaac gggctgtcaa tctcttccgc gttgataacg atcggcgcag gccagtgaac
    46681 accttccgcc aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg
    46741 aacgtgggat gcaacgacaa tgccaacatc aggcaccttt tgcagctctt caatcgaagc
    46801 ggcttccagc gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc
    46861 gacttcacgg atgccaagtg catagaggaa gcgagcaaag gtggtttctt tcgctttttc
    46921 cagcgcgtta accacgtttt gtgccaattt tggccccata cgctccagtc cggtcagttt
    46981 gcctgcggtg agtttgaaca gatctgccgg agtatggaca tattcttttt caaccagctg
    47041 atcgatgatt ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt
    47101 cagcgactct ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc
    47161 accttccaca cgctcaacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc
    47221 acgggtatct tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc
    47281 agcgcggcga atcaccactt tatcgccaat gcgtaaacca agacgttcga tttcatccgc
    47341 attgtgtaag gttgcgttac tcaccagcac gccagcaaca tggacaggtt ccagacgcgc
    47401 aacaggcgta atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat
    47461 cagctcctgc gccgggaatt taaacgctac cgcccagcgc ggggcacgcg cgacaaagcc
    47521 aagctgctcc tgctgttcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc
    47581 cagcgtcggg cggtcttctt ccactttgtg atagaacgcc agcacttctt ccgccgattc
    47641 acaaagcgtt acccgatcgc tgaccggcaa cccccacttt ttaaattgca gtaaacggcc
    47701 aagatgagta tccggcagct cgccaccttc cagaacacca acgccatagc agaaaaaagt
    47761 gagcggccgc ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt
    47821 acgtgggtta gcaaaaactt tcccacccgt gcgtcgcgca tcttcgttaa tcttttcgaa
    47881 ccccgcctgc ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc
    47941 gtgcagcttc agcggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt
    48001 ggagccatcg ccacgggtcg cggcactgac taaaacgcca ttttcataca gaatactgac
    48061 ggcaaggcca tccagcttca gctcacagca ccaggtgact ttctcgttgt ttttcagacg
    48121 gtcctgcaca cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt tatccagtga
    48181 cagcattggt acttcatggc gtatctggct gaaagctgcc agcggcgcag cacctacacg
    48241 ttgggtaggc gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc
    48301 gcgcatcagc ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag
    48361 atattcatga tggcaaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat
    48421 atcgcaccat caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc
    48481 cttaagtgta tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca
    48541 atttctgcgg agtcatcata cggcgctgat cgtcaagcac gacaccgccc acttcatcgg
    48601 caatatgctg cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg
    48661 gtacctgcat aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg
    48721 ttcccggttt caccatattc gccaggctga ataacgccgg gccgctgcca tccgggctaa
    48781 gatgacgatg gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa
    48841 gaagcagttc accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg
    48901 cttctttgcg cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg
    48961 gtgccgctac gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca
    49021 ctggctgcgg cgctggcgcg acctgtggcg aaactggctg ctggaccggt tgacgcggct
    49081 gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca gcctcatgct
    49141 cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat
    49201 catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg
    49261 gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca tgtaccagta
    49321 aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta
    49381 tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt
    49441 gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc
    49501 gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa
    49561 atacgcagaa ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat
    49621 tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc
    49681 aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg
    49741 gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactttc atgagttacg
    49801 ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc atctctgtgc
    49861 tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg
    49921 gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga
    49981 ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgtct
    50041 ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt attgggcaaa
    50101 ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg
    50161 attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac
    50221 gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgctgc
    50281 ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct
    50341 atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt
    50401 gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc
    50461 tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt
    50521 tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga agataactcg
    50581 ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg
    50641 gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg
    50701 atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccgacc
    50761 agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc
    50821 ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca
    50881 aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa
    50941 attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac
    51001 cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt
    51061 gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt
    51121 aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca
    51181 gttatcgccc aggtgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt
    51241 attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc
    51301 atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga aggtattctt
    51361 gcaggtatct cttctggagc agctgttgcc gcagcgttga aactacaaga agatgaaagc
    51421 tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta tttaagcacc
    51481 gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa
    51541 aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc
    51601 tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg
    51661 cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc
    51721 ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc
    51781 aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca
    51841 ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg
    51901 gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc
    51961 ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca
    52021 acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag
    52081 gtaccgttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg
    52141 ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta
    52201 aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct
    52261 gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttgc
    52321 tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat
    52381 caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat
    52441 tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta aagataaaca
    52501 catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
    52561 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct
    52621 gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat
    52681 tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct
    52741 gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct
    52801 ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta
    52861 tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga
    52921 taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
    52981 agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac
    53041 ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac
    53101 tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta
    53161 caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg
    53221 cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg
    53281 gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc tccgcgctat
    53341 cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
    53401 agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg
    53461 taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac
    53521 aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca atgatttaac
    53581 gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat
    53641 gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa
    53701 atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg
    53761 gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
    53821 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac
    53881 aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc
    53941 cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga
    54001 aatctctggt ttccgacgac aaaaaggata ccggaactat tgagatcatt gctccgctct
    54061 ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg
    54121 gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggtacca
    54181 ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc ggcgttgaac
    54241 tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg
    54301 ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc
    54361 tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca
    54421 aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt atccgcatca
    54481 agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg cgccattttt tatgcttccg
    54541 ccagcggcgg caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca
    54601 gtacgcgcaa ccccgcacgg tgcacagcat cgtttaacgc cttccctttc agcaaaccac
    54661 tgataagctg agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat
    54721 gggaaatgac attcacgctg tcggcactga ccaccaccac ctgcatctcc tgattttctt
    54781 cattaccgga ggcgctggta atcaccaccc attttaatgt gtctgaaagc agactttttg
    54841 cggcagcaat ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccagctcaa
    54901 agatattggg ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcctcgggaa
    54961 ggtcaggttt gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca
    55021 ataggtcagg atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg
    55081 atgccgttcc catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct
    55141 gaagcgcacg caaatagccg ctaaaccatt cgtccggaat cgcaccaccg tagaaagtgt
    55201 cataatgcgg cgtattgctc agcaataccg tcggcacggc aaagacattc aagccgttct
    55261 gttttatagc aggcaccgca atgctgttgc ctacgcttcc gtacactacc tgcgactgca
    55321 cggcgacgat atccgcctgc agcgccctac tcttatcgtt aaacaacaac aaactactca
    55381 tttaattttt tctccttgcc gatgatcctc atcgtattcc aaccgaaact ttacctgatt
    55441 ctggcagtca aatcggctat tacaaaacaa agataaggta attcagtgaa gaaaatcatt
    55501 tgtctggcca ttacgctgct aatgacactc cccgcttacg cgaagttaac tgttcatgaa
    55561 gaagcccgca tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg
    55621 cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc acctgcgtct gaagctcggc
    55681 aatacccgta accgcattga cactgccgag cagtttattg ataaggtagc ttcgtcgtca
    55741 tcgattaccg ggaagccgta tatcgtgaag atccccggta agagcgatga gaacgcacag
    55801 ccttttttac atgcgttaat tgcgcagacg gataaaacgg tgcctgcgga aggaaattaa
    55861 tccgctttgg taaggcattt acaggaggta acatgaaaaa atgctttatt tatcacgatg
    55921 aaaaatcgaa taaattttgg tggatagatt acgaagggga tagtttagct gtcaactatg
    55981 gcaaggtagg tagtattggt aaattccata caaaagaatt cgataatgaa gaacagtgtc
    56041 tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa aggatatcaa gaaactccag
    56101 actttaactt catggatctc tactattttg atgatgaaga gattgggtta catcttaaaa
    56161 cgtcacaccc aaacttccag tgccatttta ctgatccact ttatatgtgt tgctgggatg
    56221 aagaatctcc ttttggtagc gatgaaggtg ctgatgctct aaacgaactt gaaaatagcc
    56281 tccgttctgg actgtgctga tttccctcaa atgttaattg aaactatatg gggtatgaaa
    56341 tacatcgcta tggacagtat tcttgaagag gatgtgcgtg cgcaattact agccgatgaa
    56401 atgagctcta tccagagcaa tatgattacc tacgcgactg cattcggtca gatcaaagtc
    56461 atgggtaaaa tctcccataa acttaaaaag atgggactca atgcactagc gcgtcatcag
    56521 cttaccgcaa aaattcttca atggggtgac ggtcaggact caccaatact tcaaaaaatg
    56581 attgatgacc ttactgcttt tccacacgaa aattaaaaac tacatttgtc ggcagcaaca
    56641 actgccgaca aatcagatta ttgacaaagt gcactttgtt tatgccggat gcggcgtaaa
    56701 cgcctgatcc ggcataaaaa tattgctaat tcaatatatt gcagaaacat tgtaggcctg
    56761 ataagcggag cgcatcaggc agttttgcgt ttgtcatcag tctccaatgc tattaatcct
    56821 taaatccctg ccccctgact aaaatgctct tccccaaaca ctccggtgga gaggtagcga
    56881 tcgccacgat cgcagatgat cgctaccacc accgcaccag ggttagcttt tgccacccgc
    56941 agtgctccag caaccgcgcc gcctgagctg acgccacaga atattccttc ccgcaccgcc
    57001 agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat ccagcacctc atccaccagc
    57061 gcagcgttga aaatccccgg cagatattcc gcaggccagc ggcgaatgcc cggaatactg
    57121 ctgccttctt ccggttgcag gccaacaatg gtcaccggtt tggattgttc gcgcataaag
    57181 cgtgaaacgc cggtgatagt gccggtcgtc cccatgctgg agacaaaatg agtgatgcgc
    57241 ccgccggttt gctgccagat ttccggcccg gtggtggtgt aatgcgcata agggttatcg
    57301 ggattattga actgatcgag cagctttcct tcgccacgat tcgccatctc cagcgccaga
    57361 tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa tcagttccgc accataagca
    57421 cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca tcagcaattt catgcgatag
    57481 cctttcagcg cggcaatcat tgccagcgca atgccggtgt taccactggt ggcttcgatt
    57541 aagacatcac ccggtttaat ttccccgcgc ttttccgcct cgacgatcat cgaaagtgcc
    57601 gcacgatctt tcaccgaacc tgccgggtta ttgccttcca gttttaacca cacttcactg
    57661 ccgttatccg gccccattcg ctgcaacttc accagaggcg tattgcctat tgtttgttct
    57721 aatgtactca cgatctctat ccatacgtgg tgttgcctga tgcgacgctt gcgcgtctta
    57781 tcaggcctac aggttacaaa ccttgccatt aaaaagcccg gatcgcggga agcgcctccg
    57841 ggcgtttaac attcactcaa cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg
    57901 ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt tgcagaccaa cgaataaacg
    57961 ctcgccacgc tgcggggcat cgtcgccatg catcacgacc gtcagcggtt cgttgtacca
    58021 ccccagcggc tgcaccacta attgggtgta gtgacctttc gggctggctt ccagtacctg
    58081 taccggcagc ggcgaatcga gactggtacg gcggctgata tccacttccc acgggcgcag
    58141 gaagagatcc accggcccct gatacgcagg tgtgtagccc agcggccagc gatgcgcgcc
    58201 aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg ttcacttcgc ccataaattc
    58261 gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc ggcgcatcag cctgttcaat
    58321 attcccctga ctcatcacca ctacacgatc ggctacttcg gtcgcctctt cctggtcgtg
    58381 ggtcacaaac acgctggtga atttcagctc ttcatgcagt tgacgcagcc agcgacgcag
    58441 ctctttacgc acctgcgcat ccagcgcgcc aaacggttca tcaagcagca gaatttgcgg
    58501 ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc tggccgccgg aaagctgcgc
    58561 cggataacga tccgccagat gcgcaagctg gaccatttcc agcaattttg tcactttcgc
    58621 tttgatggct gcggcattcg ggcgctcgcg acgcggcagc accgtcaggc caaaagtgat
    58681 attgtcgaat accgtcatat ggcggaacag cgcgtaatgc tggaacacga aaccgacttt
    58741 acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg aagcgaatat gcccgctggt
    58801 ttgatgctcc agcccggcga taatccgcag cagcgtggtt ttcccggaac cggacggccc
    58861 cagcaacgcg accatctgac cagaaggaat atccagtgag atatcgttca gcacctgggt
    58921 gcgaccaaaa gacttcttga tattggcaat ctcaatgctc atgatgctcc tcctgctgcg
    58981 cgcgtttttc ctgattctcc aggcgccact gcaacatact ctttaaaaac agggtgataa
    59041 tcgccatcag cgtcaacagc gcggcggcgg taaaggagcc gacggtgttg tagtcctgct
    59101 ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc gcgaatcgag ccggaaacca
    59161 ccgacacagc accaaactcg ccaatcgcgc gggcgttggt cagcaccacg ccgtaaagca
    59221 gcgcccagcg aatattcggc aatgtgacgc gacggaacat ctgccagccg gacgcgccaa
    59281 gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa catcaccggc accagctcgc
    59341 gcaccacaaa cggacacgtc acaaagatgg tgaccagcac cattccgggc caggagaaca
    59401 taatttgcag attatgttca tccagccagc cgccgagcgg gccgttggag ccgtagaaca
    59461 gcagatacac cagaccggca accaccggcg atacggcaaa cggaatgtcc agtagcgtca
    59521 gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag ccaggccagc agaatgccga
    59581 acaccaggtt taccggtacg gcaatcagcg cgatcatcac cgtcagccag atggcgtgca
    59641 gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag ccccttgctg aatgcctgca
    59701 cgaagatgta aatcatcggc accagcagga tgaacgccga aaccagcatc ccgatgccaa
    59761 tcagaaacca tttgccccag ttaatcgggc gcgcgtcata acgcttcaat tgggtaactt
    59821 ccgccattaa tgacctacca cacgccgacc aaagcgactt tgcagagtgt taattgagaa
    59881 cagcagcagc agagatgccg cgaggatcac cgaagcaatc gcgctcgctg ccgggtaatc
    59941 aaactcctgt aagcgcacaa aaatcatcag cgacgtcact tccgtcttcc acgcgatatt
    60001 tccggcgata aaaatcaccg cgccaaattc accaagacta cgggtaaacg acagcgccac
    60061 gcccgccacc agcgccggag aaagctccgg cagcaccact ttgcagaaac tctgccagcg
    60121 cgttgcacca agcgtttccg ccgcttcttc atattccggg cctaactctt ccagcaccgg
    60181 ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc atcgccaccg caataccaag
    60241 ccaggtatag gtgactttga tatcaaactt cgccagccat tcaccgtaaa aaccgtttac
    60301 ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc
    60361 catcagcgca tcaagcagcg tgcggcccgg aaagcgataa cgtgttaaaa tccacgccat
    60421 cagcagaccg aaaacgccgt taaaaatcga tgccacaaac gccgacagca gcgtcacttt
    60481 gtaggccgcg accacctgcg ggttggtgat cacctcccag tactgcgccc agctcatctc
    60541 agaaagctgc atcaccagcg cagagagcgg cagcagcaat atcaggcaca caaacagcag
    60601 actggtgcca aggcttaaag taaagcccgg cagcacgcgt cttgaagaaa ctgcaaacat
    60661 tacttacgcc ccgccgctaa cagcttgtct aactcgccgc cgctggtgaa gtgggttttc
    60721 atcacttccg gccatgagcc aaatttgtct tccacgcgga acagttcggt ctgcgggaat
    60781 ttatctttca gtttgtccat gacttccggg ttattcacac ggtagtaata gtcggtgatg
    60841 atggtctgcg cctgtgggct gtagagccag ttcaggtagg cttttgcggc tttttccgta
    60901 ccgttggcct gcacattttt atcgacccac gccaccggga attccgccag aatattggtt
    60961 ttcgggatca ccacttcaaa gccctgtgct tcatactgtt tacggatgtt gttcacttcc
    61021 gattcaaagc taatcagcac atcgcccaag ccacgctcgg caaaagtggt ggttgcgcca
    61081 cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt
    61141 ttggcttcat cgccaccgtc agctttatcc gctgcgcccc atgccgccag ataggtataa
    61201 cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct tcacgtcgga gcgcaccagg
    61261 tcgttccagt cgtggatatt cttcgggttg cccttacgca ccaggaagcc catggtggag
    61321 tagaacggcg agctattatt cggcaggcgc gactgccagt cagccgggat cagcttgcct
    61381 ttgtcgtgca ggatttgtac gtcggtcacc tggttataag tgacaacgtc tgcttttaag
    61441 ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat gagattgttt tatcgtcagt
    61501 ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg gcggattcag ggcggcaaac
    61561 agctcgcggg agacgtcata agaactgttc agcagttccg ttgcctgtac atggcccgcc
    61621 agcagcagag aagcgaccag cgcgagtgag ttctttttca gtaagttaac ggccattgcg
    61681 cacccttata aatttaatga ctttctaata gccatcatat ttataacgga cgtgaaagga
    61741 gtaacgattt tatataccgt ttggtgattt ggaagttaaa aagggaataa gaacttggtt
    61801 caacaggcgt aattgttgca ggcagtttgg acacagacag cgcggagaaa ccggagcgta
    61861 cacatagtac gtgaggattt cgagcactgc ccggggccaa aatgacaaat gaaatagcct
    61921 ggtgaactta gttcaagacg aaatcctccc cacacatgca gggaggaaac agaggtgaat
    61981 cagataccga cgctaaccgt ctccggcagt gtgctgccgc catcaatcac attctgtgta
    62041 ccggttaaat agctggattc atccgatgcg aggaaggccg ccagttcgcc gacttccagc
    62101 ggatcggcga ggcgacacag cgggattgct tttgccattt cagtcagcac cgattctgga
    62161 tcttccgggt tcgactggcg ggcaatgctt tccgccattg gcgtgcggac gtatcccggg
    62221 cagatggcgt taacgcgaat accggattgc gcgtactcca ccgccagcga tttagtcagg
    62281 ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc caggatcggc caccatatca
    62341 ccagtgactg aagacatcat cacaatgcga ccatctttgc gcgcaatcat ctccggcagc
    62401 accgccttcg tgacgttcca tacgccttta atattgatat caatatggaa atcgcgatct
    62461 tcatcgctca tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt cactaggata
    62521 tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg cggctaccga cgccgggtca
    62581 cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac cacacagttc gtccgctagc
    62641 ttttcgatct caggggagat atccagcaag attaggttcg cgccatgacg tgcaaaagtt
    62701 ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg
    62761 agtttaccca ttttaatgct ctccttgtta atacagagcg ttacactcca ttccctctga
    62821 aaatacagga taaacaaaat attgctgcgg atcagctcac taattcagga aaatctcaat
    62881 ccagtcgctg ctttatgcat catttcaggc gttgtgttag ctcactgctg cgctgaatca
    62941 ttttcaatga cgccacatca ttgatgttgc acaagaccaa caaacagtaa atcggtaaca
    63001 gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc accggaaacg
    63061 gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga agtaatggca
    63121 atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc ttttttactg
    63181 ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga aacggtggct
    63241 tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa ttttcatcag tttgaacgac
    63301 agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta tctggataaa cggtgcttta
    63361 ctgatgacct caatgatttt ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc
    63421 agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga atcatcactg
    63481 gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct ggcactgtat
    63541 tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt ctgggcaaat
    63601 ttcacaatgc tcgactggct gataccgagc tgtttcgcca tctgccgtga agagacggat
    63661 ttcagctcgc tcactctggc ccgcagaaag tcagcaattt tttgctcgtt cccggtaaat
    63721 tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc tgggctggca
    63781 gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt agactcaatg
    63841 attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat tatttgatcg
    63901 taaatagtaa ggtcaccacc gatgcaacta gaaaagatga ttactgaagg ctcgaacacc
    63961 gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat caacgatgaa
    64021 gataaaaccg taccgcttgc cgttgatcgc gtcctgccgg atatcgccgc ggcgatcgat
    64081 gttatccacg cccaggtcag cggcggcggt cgtctgattt atctcggtgc gggaacatcc
    64141 ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt
    64201 ctggtggttg gtttgattgc tggaggcgaa tatgccattc agcacgcggt ggaaggcgct
    64261 gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata ttaatttaac ggcacaggat
    64321 gtggtggttg gcattgctgc cagcggtcgc acgccgtatg tgattgccgg actggaatac
    64381 gcacgccagc tcggctgccg cacagtggga atttcctgta atccggggag cgccgtttca
    64441 accaccgctg agtttgccat tacaccgatt gtaggtgccg aagttgttac cggttcttcg
    64501 cggatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac cgggctgatg
    64561 attaaatccg gcaaagtatt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa
    64621 ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc cgaacaagcg
    64681 gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa
    64741 aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt tattcgtcag
    64801 gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct gaacaccatt
    64861 cttacccgtg tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg
    64921 cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct ggaaggcgtg
    64981 aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg taaagcgcat
    65041 cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga tgccgcagaa
    65101 atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt
    65161 ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc cgccggtctg
    65221 ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc
    65281 acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt caccttccta
    65341 gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt caatggcgca
    65401 attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg ttactacgcc
    65461 ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat cggcgtgctg
    65521 attgccgcga gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat gccggacgat
    65581 ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct tgcgtacctg
    65641 atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt tatgcacctg
    65701 aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt
    65761 ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca gggattcaac
    65821 agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc gctggcactc
    65881 tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc gattattccc
    65941 ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg catgaagccg
    66001 tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggatt gatagcctgg
    66061 tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg
    66121 ctgatgactt ccgcacaagg catccttccg gcaatggcgg tttatgctgg cgggattctg
    66181 gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa cgtcaatctg
    66241 gactgatatt atgaaacgga caatgctcta tctttctctg ctggctgtta gttgtagcgt
    66301 cagcgccgca aaataccctg ttctgacaga aggtagttga tcaagtatat gcggcgttaa
    66361 agcagtaata aaaaggccag tcagtaatgt tactgcctgg cctttaatca atgaatcaga
    66421 aacgcttaca gcaccatcaa cttgtccagc gacggtgcga aataatagcc gccggttacc
    66481 ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg tatcgccaaa catgctcagc
    66541 agttgctgct caatgttatg cagacgcgcg cagtaggcgc agaagtacag accgtgagtg
    66601 ccactggcgg tgccgtacgg caggctctgg cgaacaatct tcagcccttt gccatcttct
    66661 ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt cgtcgccgtc aatctcttcg
    66721 ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt gaacgctcat ccggttgagc
    66781 tgcttcaggt tgtgctccca acgctgcaca aacacatagc tgccgccggc atccacgccg
    66841 tctttgataa ccgccacttc gcggcgcgtc tcttcacccg ccgggttttc tgtaccgtca
    66901 acaaagccgc tcaggtcacg ctcttccacc caacggaagc cgtggatctc ttctttcact
    66961 tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga cagagaagtt tacgtcgtga
    67021 cgcagagaaa gaatgtggat caacacatcg aactgggtcg tcggcgcaag gcctttaccg
    67081 tagcccggaa aatctttcag ctcttctgcc ccaacgccgc cgctcagagc gcgccaggtg
    67141 ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg ggaatttcgc ttcaaaagtt
    67201 gccagtttgt cggcaaatgt tttactggcc gcacgcaggg cgtcaacttc ccctttcacg
    67261 ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca aaatgccact ctgaacctga
    67321 gacattgttc ctcctgaaaa taagaatgcc acgcaatccg tggcattgtt gagcgctatt
    67381 ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa ttaacgccgc cagataattt
    67441 tactgacttt ccagtttttc agggtatcgt ccgaaggcat taacccttca ggaccacgcc
    67501 attccccgct gaactggtag ctaatatgtt gactgccttc ggctttacat tcgacggcac
    67561 gattatcatc accatcagct ttttggcaat tgccaaatgc tttgctgtaa aggtcgctaa
    67621 acggtgtacc gattttaaca ccggtgtcag caggaatatc gctatccagc acgtcaatgc
    67681 gactgatcgt gccttgatcg ccgttaatca ccatcgccac gttgtcgcct ttcatcactt
    67741 caaaaaagcg caccacgttg ccgttcgcgg ttttcattcc gctgcgcagg cgataatcgc
    67801 catcaagcgc atcagcaatg gcttgttctt gcagtggtgt ggacgccgtt aattcaccca
    67861 cgccctgctc gctcactttg gtggacgatc caaaccagtt ccacgggtta gcggcagacc
    67921 agttaactga cgacatcgtg gaacagccgg tcagcatcaa cggcatagcg cataacatta
    67981 aacgcagcga tttcatgtca cttcctttgg ttattcaata acgttgcttg gagtgcaaat
    68041 tcaccaaaaa gtgccgttat tctttttctt cattaaaaca agcgcgtaaa cgtcgattgg
    68101 tcagcagcca gattaacgcc acaatatccg ccactaccag cgccagaccg ataccgctaa
    68161 cggattcacc gttcagccac agccacggtt gccagcaaag taaaaccacc tgcgccaaca
    68221 gcaacagaaa atagagcaca tgccaggtgc gaggaaacga agcccgccga ccgctcaaca
    68281 aaaacgccag caccgccgga ataccgggaa tcagccccag ccagaaatta tcgtgatcgg
    68341 gataaaacag atttagcagc gcagtaccct gctcgcgcga cgcaccggca atgacaaaca
    68401 gcacccaggt tcgcgcctga agcaatagca caagccagaa gagcaagggt aaacgcaggc
    68461 gtccgtgcgc atcataatgg acaggatgga actcagtact cttcatcttc aatcaaacgc
    68521 ttacccagac taagcacgtc ggcgtgttca tatcccaggc gttcatacat tccgagcacc
    68581 atgtcgttat cttccggcac gttgatctga attttcgggc agccgcgagc aatcagcttt
    68641 ttctccagcc ggttaagcaa cgcattggca attccacgcc cacgaaactc tggatgcacg
    68701 ccaagataat aagcagaccc gcgatgcccg tcataaccgc ccatcaccgt tccgaccact
    68761 tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat ggttcatctt acgctcgatg
    68821 tccatttccg gatcgttcca cggactcagc aagtcgcaac gctcccaaag ggtgatgacc
    68881 tcttcgaaat cttcctggcg aaatacgcgt atctccatgg tattcgttac ctttttgcgg
    68941 gttaaaaggc tgattatggc gtgaacggtc gaattagcca atatctgacg aaaatcggtt
    69001 gaaaaagtgg cataatgggg agttgtcaac tattgaaatg aaaagtaaaa caattctcaa
    69061 cagcaaaccg tcgtaacgga ttacgcgata cgatataaca tctggaactt tattattaca
    69121 actcaggccg tatgagcact tttaaaccac taaaaacact cacttcgcgc cgccaggtgc
    69181 tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc gcaagccatt gccaaagacg
    69241 aacttttaaa aaccagcaac ggacacagca agccgaaagc caaaaaatct ggcggcaaac
    69301 gtgtcgttgt tctcgatcca ggccacggcg gaattgatac cggagcgatc ggacgcaacg
    69361 gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa cgtccgttcc attttgcgta
    69421 atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac gtttatccca ctttacgatc
    69481 gcgttgaaat cgcccataaa catggcgcag atctgtttat gtcaattcat gccgatggct
    69541 ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct ctctaaccgt ggggcaagta
    69601 gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc cgatgaagtt gccggtaaaa
    69661 aggcgactga caaggatcac ctattgcagc aagtactgtt tgatctggtg caaaccgata
    69721 ctattaaaaa cagcctgacg cttggctcgc atattctgaa aaagattaag ccggtgcata
    69781 aactgcacag ccgcaacacc gaacaagccg cgtttgtggt gttgaaatcg ccgtcggttc
    69841 cttcggtgct ggtggaaacc tcgtttatca ccaacccgga agaagagcgg ctgttaggca
    69901 cggcggcgtt tcgtcagaaa atcgccacag cgattgctga aggcgtgatc agttatttcc
    69961 actggttcga caaccagaaa gcacattcga aaaagcgata agttatgaaa cccgacgcac
    70021 accaggttaa acagtttctg ctcaaccttc aggatacgat ttgtcagcag ctgaccgccg
    70081 tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga agctggcggc ggcgggcgca
    70141 gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg cgtcaacttt tctcacgtcc
    70201 acggtgaggc gatgcctgct tccgccaccg ctcatcgccc ggaacttgcc gggcgcagtt
    70261 tcgaggcgat gggcgtttca ctggtagtgc atccgcataa cccgtatgtt cccaccagcc
    70321 acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc agagcccgtt tggtggtttg
    70381 gcggcggctt cgatttaacc cctttctatg gttttgaaga agacgccatt cactggcacc
    70441 gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt ttatccccgt tacaaaaagt
    70501 ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca gcgcggcatt ggcgggctgt
    70561 tctttgatga tctgaacacg ccagatttcg accactgttt tgcctttatg caggcggtag
    70621 gcaaaggcta caccgacgct tatttaccaa ttgtagagcg acgtaaagcg atggcctacg
    70681 gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg ttatgtcgag ttcaatctgg
    70741 tctgggatcg cggcacgctg tttggcctgc aaactggcgg gcgcaccgag tcgatcctga
    70801 tgtcaatgcc gccactggta cgctgggagt atgattatca gccaaaagat ggcagcccag
    70861 aagcggcgtt aagtgagttt attaaggtca gggattgggt gtaactccct cacccccatt
    70921 cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc aaacagctgc tggtaatccg
    70981 tggcaaattg ccccagatgc cagaatcccc actgcatggc ggcgtctttt accgttgtgc
    71041 tttgcgacca cgggcttatc agttcgcggc gtacggcgtt caggcgaatg cgtttcagcc
    71101 acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt ttgtagcgtg cggcgactga
    71161 catgcagttg attacacaaa tccagcaccg tcaccggttc ggacatgttt tccagcacat
    71221 attcacgggc gcgggaaagc aaccgacggt aactctgatg actgatgctt tccgccgtca
    71281 ccattggttg cgcttcttcc agcatcgccc ccatcgccat tagcaaatta tcccccagca
    71341 cttttcgcac tgcaggctga tggagatttt ccgggttctc gcaaaacgtc gccagcgcct
    71401 gttggacaaa gccccacagc gcggctttat gctgcttctt tcacttccag cgccgactgg
    71461 ctacgcagca tatgtaatac ccgatccgga ttgtgcaaaa agttagcctg acaggtgatg
    71521 acatcttcag acagcactac gcccaggatc gtataatcgt ccggcgtact taattcaaac
    71581 tcggttccac cggggcgcgt tgctatttct gcgcttccca gacattgcga accgataaat
    71641 ccctgctcac cgcgcgtcgc cggaatgcca aaccagaacg agttcggcca gaccaggcac
    71701 gactgacgca gcgccagacc ggtgtattca cgaaaaacct gaatatcatc gagtagaatt
    71761 tccgtaaatt caccatgaaa cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg
    71821 gtaatcgtca aagcatgttc atagacatcc gttgtctgtc gttgatgaac attatccact
    71881 tcgaccttcg gcgtgagctt caggttttcg ggtaagggtt catgataaag atggtgcaaa
    71941 ttggctgtac gggtcttttt catgatgtta atgccgggtg ttgtaggaca cccggcacct
    72001 ccgacaggtt aatggggctt gagacgataa cgactactgc gtttacgtta cgtcccggca
    72061 gaaaagagct gttcgagcgc atttctggct ttttccagcg gccagccaaa gtgggcggcc
    72121 acttctcctg ccgtcattcc ctgacatact gatgttaaca gcgccaacaa ctcgtcagca
    72181 gattccgcga cgataactgg cgcgtcgggt tcctttacgg gttgcttcgg ctggcggtta
    72241 aagccagtaa ccagccactg ggtgtcatcg tctggccgac cgatctcctt acggctgatg
    72301 acacgcccgg tacgcatcgc ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc
    72361 agatcgcctt ccaccaccag cgttaagcga ccagggtcaa gcacttcgtg actgagcaga
    72421 cgaacgttag ctgctttaag catggcatcc gctgcatcta tcgcggcgac catgccgtcc
    72481 acttccagca atcccagtgc attgatcatc ggcaacctcc gttacgcacg ctggattgga
    72541 ttacgcgcga tttccagcac tgcatcggta aaggcgttac aggctgcttt acacgcggcc
    72601 tggctaccgg ttaaaaatgc tgccgagtag ttggtttcag acggtggcgg gacgtaggtc
    72661 accagctgta cgtcggcaga tttcaacgcg gcatcaatgc cgtaggtcgc ttccagcggc
    72721 ggtgccacca gatacgccat cggatcgcca agcgtgatcc cggcggttga tgaaagataa
    72781 gaacctgtac gcgaaaccac atgcgccagg aaggcggtgt tttctgcgtc attggcccac
    72841 tggaaagctg cgccattttc aatatgtgca accatcgcat ccagaccggc acgcacttcc
    72901 gccgggtttg gcccaccaag cataatcagc acttcaccgg cagtcggtga cgggccgtgt
    72961 gccgctcccg catacagcga gcgaccatat accacttcga ccatcgcttg tttggtcgct
    73021 tcgtcagccg caatgtaggt tacgtcgtca gaatcggcag aaatcagccc gaggctacga
    73081 atatgcggcg gcaattttag ttcacgcgca aattcagcgt taacggaggc aatcacccgc
    73141 atggcggtta ccgacggtcg aatcaaatct aaagctggca tgatgcctcc ttatcgggtc
    73201 atgttgatgc cggatgcttt ctgctccagc atacgtttcg ccaaatccac aatgacggct
    73261 gcggcttcaa ccggcggcgt gccgccctgg tgaatgttag agatacaggt gcgatcggcc
    73321 tcaacggtgg tcgccatacg cggcgagtaa acggcgtagc aggagaggct ttctgactgc
    73381 cctaaccccg gacgttcccc caccagcagg atcaccactt tcgcgccgag gatctcaccg
    73441 atctgatctt caatcttcac gcgaccataa cgaacaaaga atggcgtgcc gactttcagc
    73501 ccggcctgtt tcaggcccgc catcagcggc ggcaggatct cttcgtagtt gacggtgatg
    73561 gcatcagttg acaggccatc agaaataacg acctgtacgt ccggattagc aacacactgt
    73621 gctttcagcg cttcaacagc ttctgcacac aggcggcggc ccatatccgg gcgagtcagg
    73681 tagagatttt tgtcgctaat ttcagagcgt acttccagca agccctgcgc tttcacccac
    73741 tcttccggca cttctttcag tacggtatct ttcgaacgag agtgatcggc caagaaacgc
    73801 agcagcgcct gagtacgtgg acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg
    73861 ctgcggcgca gttctgttaa tacgtctgcg cgatgcgggt tttcaacacc aatccacgct
    73921 tttgcttcag cggaacctaa atccagcgca cagctttccg aggtcaccgg tgccgcacag
    73981 ttggtggtgg cgcactttgc ttctgacggc gcgggggccg cttgtcccat tgacgccatc
    74041 acgctgcgta caatttcttc aatctgtttt tgatccatga tgtgttatct ccgcgtcatc
    74101 agaagaacag tgacggatcg cccgcccgtt tggtcaggcg accgtttgcc ataatgccca
    74161 tgctttccag ccagcgttca aactctggtg acgggcgcag gttgagtaac tgacgcacag
    74221 tggaggtatc gtggaatgcg gtggtctggt agttgagcat gatgtcatca cccagcggca
    74281 tccccatgat gtagttgcag cctgcggtgg cgagcaggat catcaggttt tcgttgaggt
    74341 tctggtcaga gtcagcgtgg ttggtgtagc agccgtcaca gcccatagag atgccgctca
    74401 gcttgcccat aaagtgatct tctaagccag cacggataat ctggcggtcg ttgtagagat
    74461 actccggccc aataaagccg accacggtgt tgacgataaa cggatcgtaa tgacgcgcca
    74521 gcccatagtt acgtgcttcc atcgtcacct ggtcagcgcc gaagttagcg ccagcggata
    74581 gcgcagagcc ttgtccggtt tcgaagtaga ggcagttttc cccggcgata cgattgaact
    74641 ctgcgcccac tgcgcgcgct tcgtcgagca tcgccagttc cacgccaaac tctttcagcc
    74701 ctttttcgct gccacagata ctctggaaaa tcagtccgcc aggagcgccg cgacgaatcg
    74761 cttcgatctg ggtggtgacg tgcgccagta cgcagccctg agttgggatg ttgaatttgt
    74821 cgatcacgcc ataaatggta tccagcacgc ggcttaagtt ttccacgtcg tcagttaccg
    74881 ggttaacgcc gatcaccgca tcgcccaccc cgaaggaaag cccttcatag atttgcgcag
    74941 cgatactttg cacgtcgtca cgggtatcgt tcggctgcaa acgggcgcta aaggtgcccg
    75001 gaataccgat ggtggtattg gcctttttga ttaccggcat tttcttcgcg ccgtagatca
    75061 ggtccgcgtt ggagcaaatc ttcgctatcg ccgcgaccac ttccgaggtc agccctttgc
    75121 gggtaaaggc aatgtcgtcc acgctggttt catcgctcag cacatactca cgcagttcgc
    75181 tgatgctcca gtttttaatc tggttgtagg ccgtttcgtt aacgtcgtcc tgaatcagcc
    75241 gcgtcacgca gtcatcttca taggcaatca ccggattatt gcggatgtcc gctacggtca
    75301 tttccgacaa cacctgcttt gccgccacgc gctcctgtga gcttgccgct gcaacgcccg
    75361 ccagcacatc ccccgaacgc agttcgttgg ctttagccag cacctctttt acatccttaa
    75421 actgatatac attgccgaac aatgtggtct ttagtttcat aagtcgttcc ctcaggaaga
    75481 agtgatgagt aacatttctc acagcctgca aacaattcta tttcatcgtt agcccttgtc
    75541 atgtaaggtg atttccttaa cacgcccgtt acttatcctg ccgaacgcaa ccgaactcgc
    75601 cggatacatg agcggggtta tcgagtcgtt agatcgaggt atccggtttt gagcaaaaca
    75661 ccactcacag caaaggccat agacgccgca caaccaaagg acaagcaata caaactcacc
    75721 gcttcactga cgcccggcct gtttttgctg gtgcatccca acggcagcaa atactggcga
    75781 ttccggtact ggatagacaa gaaagagcgg ttacaggcta ttggggtata tccgctgatt
    75841 agcctcaagg aagcccgcaa acgcgccaca gagagccgtt tactgatagc ccagggaatt
    75901 gacccaatgg atttggcacg caaggagaaa gccattgatg cactcaacat ggcggcaggt
    75961 tttaagacgg tagcggagga ctggctggct accagggtag gcggctggag tgagagctat
    76021 acaaagcagg tcaaatcagc actggagaaa gacgtttatc cggtgcttgg caagcgttca
    76081 atcgttgata taaccgcccg tgacgtgctg gcattactcc agaagaaaga acgtaccgca
    76141 ccggagcagg cccgcaagct acgccagcgc atcggtgaga tcttcaagtt tgccgttatc
    76201 accgaactgg taacgaggaa tccggttgca gatcttgata cggcactgaa ggctcgccga
    76261 cctggtcaca acgcgtggat accgattagt gaaattccgg ccttctacaa agcccttgag
    76321 agggccggga gcgtccagat tcaggcggca atacgtttgc ttatattaac ggcattaagg
    76381 accgcagaac tgcgtttgtg ccgttgggaa tgggtaaacc tggaggacgc aacaatcaca
    76441 ttgcccgctg aagtcatgaa ggcccgccgc ccgcatgtgg tcccgctatc acggcaggcg
    76501 gtcgagctat tgcaggacca gttcacacgc agcggataca gtgttttcgt gttcccgggc
    76561 cgattcatgg ataagccatt atcagccagc gcgatactca aggcgcttga gcgtatcgga
    76621 tacaagtcaa tagccacagg ccacggctgg cgcacaacgt tcagcaccgc acttaacgaa
    76681 agcggcaggt ataaccctga ttggattgag atccaactag cccacgtccc gaagggaatt
    76741 aggggagttt acaaccaagc ggcctatctc aaaatgcgcc gtcaaatgct tcaggactac
    76801 gccgacgcga ttgattcgat actggctgat ggtatcaatc cgcttgaact gtaatgaatg
    76861 attaatcacg ttttccgacg actccaccgg agtaggccgg ataaacactg gacaccttca
    76921 aaataactat tcacttttgc cggattcgtc cggctttttt tgtctttgcc gatggga
//
LOCUS       DADWTH010000004        76412 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.004, whole genome shotgun sequence.
ACCESSION   DADWTH010000004 DADWTH010000000
VERSION     DADWTH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.004"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..545
                     /locus_tag="JNY02_000252"
     CDS             <1..545
                     /locus_tag="JNY02_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBB9431927.1"
                     /translation="FPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKEN
                     SLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVI
                     EQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASY
                     TQLEIKHSHPKNRASGKRVS"
     gene            965..1580
                     /locus_tag="JNY02_000253"
                     /pseudo
     CDS             965..1580
                     /locus_tag="JNY02_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1683..2186
                     /locus_tag="JNY02_000254"
     CDS             1683..2186
                     /locus_tag="JNY02_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBB9431928.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="JNY02_000255"
     CDS             complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="JNY02_000255"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBB9431929.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="JNY02_000256"
     CDS             complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="JNY02_000256"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBB9431930.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="JNY02_000257"
     CDS             complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="JNY02_000257"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBB9431931.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="JNY02_000258"
     CDS             complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="JNY02_000258"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBB9431932.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="JNY02_000259"
     CDS             complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="JNY02_000259"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBB9431933.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="JNY02_000260"
     CDS             complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="JNY02_000260"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBB9431934.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="JNY02_000261"
     CDS             complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="JNY02_000261"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBB9431935.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="JNY02_000262"
     CDS             complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="JNY02_000262"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBB9431936.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="JNY02_000263"
     CDS             complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="JNY02_000263"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBB9431937.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="JNY02_000264"
     CDS             complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="JNY02_000264"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBB9431938.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="JNY02_000265"
     CDS             complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="JNY02_000265"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBB9431939.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16156..16818)
                     /gene="nuoB"
                     /locus_tag="JNY02_000266"
     CDS             complement(16156..16818)
                     /gene="nuoB"
                     /locus_tag="JNY02_000266"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBB9431940.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="JNY02_000267"
     CDS             complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="JNY02_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBB9431941.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="JNY02_000268"
     CDS             complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="JNY02_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBB9431942.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19776..20993
                     /gene="alaA"
                     /locus_tag="JNY02_000269"
     CDS             19776..20993
                     /gene="alaA"
                     /locus_tag="JNY02_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBB9431943.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21077..21676
                     /gene="yfbR"
                     /locus_tag="JNY02_000270"
     CDS             21077..21676
                     /gene="yfbR"
                     /locus_tag="JNY02_000270"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBB9431944.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21735..23567)
                     /locus_tag="JNY02_000271"
     CDS             complement(21735..23567)
                     /locus_tag="JNY02_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBB9431945.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="JNY02_000272"
     CDS             complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="JNY02_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBB9431946.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24315..24809)
                     /locus_tag="JNY02_000273"
     CDS             complement(24315..24809)
                     /locus_tag="JNY02_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBB9431947.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24892..25347)
                     /locus_tag="JNY02_000274"
     CDS             complement(24892..25347)
                     /locus_tag="JNY02_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBB9431948.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25685..26887
                     /gene="ackA"
                     /locus_tag="JNY02_000275"
     CDS             25685..26887
                     /gene="ackA"
                     /locus_tag="JNY02_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBB9431949.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26962..29106
                     /gene="pta"
                     /locus_tag="JNY02_000276"
     CDS             26962..29106
                     /gene="pta"
                     /locus_tag="JNY02_000276"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBB9431950.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29296..30816
                     /gene="yfcC"
                     /locus_tag="JNY02_000277"
     CDS             29296..30816
                     /gene="yfcC"
                     /locus_tag="JNY02_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBB9431951.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="JNY02_000278"
     CDS             complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="JNY02_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBB9431952.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="JNY02_000279"
     CDS             complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="JNY02_000279"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBB9431953.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="JNY02_000280"
     CDS             complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="JNY02_000280"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBB9431954.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32836..33483
                     /gene="yfcG"
                     /locus_tag="JNY02_000281"
     CDS             32836..33483
                     /gene="yfcG"
                     /locus_tag="JNY02_000281"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBB9431955.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33540..33902
                     /gene="folX"
                     /locus_tag="JNY02_000282"
     CDS             33540..33902
                     /gene="folX"
                     /locus_tag="JNY02_000282"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBB9431956.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIVREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33923..34816
                     /locus_tag="JNY02_000283"
     CDS             33923..34816
                     /locus_tag="JNY02_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBB9431957.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34864..35754)
                     /gene="rpnB"
                     /locus_tag="JNY02_000284"
     CDS             complement(34864..35754)
                     /gene="rpnB"
                     /locus_tag="JNY02_000284"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBB9431958.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="JNY02_000285"
     CDS             complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="JNY02_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBB9431959.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36732..37448)
                     /locus_tag="JNY02_000286"
     CDS             complement(36732..37448)
                     /locus_tag="JNY02_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9431960.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37445..38131)
                     /gene="hisQ"
                     /locus_tag="JNY02_000287"
     CDS             complement(37445..38131)
                     /gene="hisQ"
                     /locus_tag="JNY02_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBB9431961.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="JNY02_000288"
     CDS             complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="JNY02_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBB9431962.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39224..40006)
                     /gene="argT"
                     /locus_tag="JNY02_000289"
     CDS             complement(39224..40006)
                     /gene="argT"
                     /locus_tag="JNY02_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBB9431963.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40272..40841)
                     /gene="ubiX"
                     /locus_tag="JNY02_000290"
     CDS             complement(40272..40841)
                     /gene="ubiX"
                     /locus_tag="JNY02_000290"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBB9431964.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40936..42453)
                     /gene="purF"
                     /locus_tag="JNY02_000291"
     CDS             complement(40936..42453)
                     /gene="purF"
                     /locus_tag="JNY02_000291"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBB9431965.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEEENLEMHNEG"
     gene            complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="JNY02_000292"
     CDS             complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="JNY02_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBB9431966.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="JNY02_000293"
     CDS             complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="JNY02_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBB9431967.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43889..45157)
                     /gene="folC"
                     /locus_tag="JNY02_000294"
     CDS             complement(43889..45157)
                     /gene="folC"
                     /locus_tag="JNY02_000294"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBB9431968.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45227..46141)
                     /gene="accD"
                     /locus_tag="JNY02_000295"
     CDS             complement(45227..46141)
                     /gene="accD"
                     /locus_tag="JNY02_000295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBB9431969.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46297..46956)
                     /locus_tag="JNY02_000296"
     CDS             complement(46297..46956)
                     /locus_tag="JNY02_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBB9431970.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47039..47851)
                     /gene="truA"
                     /locus_tag="JNY02_000297"
     CDS             complement(47039..47851)
                     /gene="truA"
                     /locus_tag="JNY02_000297"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBB9431971.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47851..48864)
                     /locus_tag="JNY02_000298"
     CDS             complement(47851..48864)
                     /locus_tag="JNY02_000298"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBB9431972.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="JNY02_000299"
     CDS             complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="JNY02_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBB9431973.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50165..51160
                     /gene="flk"
                     /locus_tag="JNY02_000300"
     CDS             50165..51160
                     /gene="flk"
                     /locus_tag="JNY02_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBB9431974.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMPTRPALWILLVAIILMLVWLVR"
     gene            complement(51157..52335)
                     /locus_tag="JNY02_000301"
     CDS             complement(51157..52335)
                     /locus_tag="JNY02_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HBB9431975.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="JNY02_000302"
     CDS             complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="JNY02_000302"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBB9431976.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDITGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53989..55995
                     /gene="mnmC"
                     /locus_tag="JNY02_000303"
     CDS             53989..55995
                     /gene="mnmC"
                     /locus_tag="JNY02_000303"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBB9431977.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(56116..56394)
                     /locus_tag="JNY02_000304"
     CDS             complement(56116..56394)
                     /locus_tag="JNY02_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBB9431978.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56428..56976)
                     /gene="epmC"
                     /locus_tag="JNY02_000305"
     CDS             complement(56428..56976)
                     /gene="epmC"
                     /locus_tag="JNY02_000305"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBB9431979.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56976..57785)
                     /locus_tag="JNY02_000306"
     CDS             complement(56976..57785)
                     /locus_tag="JNY02_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBB9431980.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="JNY02_000307"
     CDS             complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="JNY02_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBB9431981.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSPDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="JNY02_000308"
     CDS             complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="JNY02_000308"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBB9431982.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="JNY02_000309"
     CDS             complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="JNY02_000309"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBB9431983.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60831..61382
                     /gene="smrB"
                     /locus_tag="JNY02_000310"
     CDS             60831..61382
                     /gene="smrB"
                     /locus_tag="JNY02_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBB9431984.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61486..62376)
                     /locus_tag="JNY02_000311"
     CDS             complement(61486..62376)
                     /locus_tag="JNY02_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBB9431985.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(62363..62887)
                     /locus_tag="JNY02_000312"
     CDS             complement(62363..62887)
                     /locus_tag="JNY02_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9431986.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62884..63354)
                     /locus_tag="JNY02_000313"
     CDS             complement(62884..63354)
                     /locus_tag="JNY02_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9431987.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDIAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(63351..63899)
                     /locus_tag="JNY02_000314"
     CDS             complement(63351..63899)
                     /locus_tag="JNY02_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9431988.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63904..64626)
                     /locus_tag="JNY02_000315"
                     /pseudo
     CDS             complement(63904..64626)
                     /locus_tag="JNY02_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64646..65368)
                     /locus_tag="JNY02_000316"
     CDS             complement(64646..65368)
                     /locus_tag="JNY02_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBB9431989.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(65428..67287)
                     /locus_tag="JNY02_000317"
     CDS             complement(65428..67287)
                     /locus_tag="JNY02_000317"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HBB9431990.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(67369..67932)
                     /locus_tag="JNY02_000318"
     CDS             complement(67369..67932)
                     /locus_tag="JNY02_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBB9431991.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68616..69101)
                     /gene="sixA"
                     /locus_tag="JNY02_000319"
     CDS             complement(68616..69101)
                     /gene="sixA"
                     /locus_tag="JNY02_000319"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBB9431992.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(69304..71448)
                     /gene="fadJ"
                     /locus_tag="JNY02_000320"
     CDS             complement(69304..71448)
                     /gene="fadJ"
                     /locus_tag="JNY02_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBB9431993.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(71448..72758)
                     /gene="fadI"
                     /locus_tag="JNY02_000321"
     CDS             complement(71448..72758)
                     /gene="fadI"
                     /locus_tag="JNY02_000321"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBB9431994.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72938..73222)
                     /locus_tag="JNY02_000322"
     CDS             complement(72938..73222)
                     /locus_tag="JNY02_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBB9431995.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73594..74934
                     /gene="fadL"
                     /locus_tag="JNY02_000323"
     CDS             73594..74934
                     /gene="fadL"
                     /locus_tag="JNY02_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBB9431996.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75300..75584
                     /locus_tag="JNY02_000324"
     CDS             75300..75584
                     /locus_tag="JNY02_000324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431997.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQ"
     gene            75677..76351
                     /locus_tag="JNY02_000325"
     CDS             75677..76351
                     /locus_tag="JNY02_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9431998.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAKYQNEINQLSALG
                     VNFDDI"
BASE COUNT        19318 a        20452 c        18739 g        17903 t
ORIGIN      
        1 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
       61 aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc
      121 atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggag
      181 aaatgtcgaa taataatata aaagttcatt taaatgaacc agagataata tttttacatg
      241 ccgatcttca gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg
      301 aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact
      361 atgctgacag atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc
      421 gactggtaaa cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt
      481 atacacaatt agaaataaaa cactcacacc ccaaaaatag agcatccggc aaaagagtga
      541 gttaacatca ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa
      601 tttgctaaaa agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg
      661 gtatttccag cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg
      721 ctgacttttc ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa
      781 cataattata tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa
      841 aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata
      901 aataatgatg aaataacgcc ttttattgat aatttacata tgaaaaaatt tttccttttg
      961 ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt
     1021 atcaccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac
     1081 acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta
     1141 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
     1201 gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt
     1261 atcccgatcg gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc
     1321 gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc
     1381 caaggtttat tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc
     1441 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
     1501 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct
     1561 tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
     1621 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
     1681 aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg
     1741 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
     1801 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
     1861 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg
     1921 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
     1981 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
     2041 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
     2101 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
     2161 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
     2221 gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca
     2281 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
     2341 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
     2401 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
     2461 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
     2521 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
     2581 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
     2641 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
     2701 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
     2761 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
     2821 agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag
     2881 gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
     2941 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
     3001 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
     3061 ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc
     3121 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
     3181 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
     3241 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
     3301 aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg
     3361 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
     3421 ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
     3481 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
     3541 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
     3601 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
     3661 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
     3721 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
     3781 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
     3841 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
     3901 ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc
     3961 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
     4021 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
     4081 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
     4141 cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag
     4201 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt
     4261 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
     4321 gtctgagcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg
     4381 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg
     4441 taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg
     4501 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
     4561 atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc
     4621 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
     4681 atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc
     4741 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
     4801 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
     4861 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
     4921 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
     4981 agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
     5041 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
     5101 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
     5161 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
     5221 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
     5281 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
     5341 atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca
     5401 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
     5461 ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa
     5521 gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
     5581 ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc
     5641 gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag
     5701 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
     5761 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
     5821 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
     5881 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
     5941 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
     6001 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
     6061 gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca
     6121 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
     6181 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
     6241 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
     6301 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
     6361 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
     6421 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
     6481 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
     6541 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
     6601 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
     6661 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
     6721 acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta
     6781 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
     6841 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
     6901 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
     6961 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
     7021 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg
     7081 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac
     7141 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
     7201 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
     7261 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
     7321 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
     7381 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
     7441 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
     7501 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
     7561 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
     7621 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
     7681 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
     7741 cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg
     7801 ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga
     7861 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
     7921 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc
     7981 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
     8041 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
     8101 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
     8161 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
     8221 gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt
     8281 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
     8341 gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg
     8401 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca
     8461 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
     8521 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
     8581 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
     8641 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
     8701 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
     8761 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
     8821 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
     8881 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
     8941 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
     9001 tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata
     9061 aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc
     9121 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
     9181 tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt
     9241 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
     9301 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc
     9361 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
     9421 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
     9481 ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt
     9541 tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg
     9601 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
     9661 tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca
     9721 agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtgttgc
     9781 catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg
     9841 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
     9901 attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct
     9961 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac
    10021 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    10081 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
    10141 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    10201 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    10261 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg
    10321 ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca
    10381 cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga
    10441 cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt
    10501 cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg
    10561 cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
    10621 gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca
    10681 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    10741 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
    10801 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
    10861 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    10921 cgattttgtt ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg
    10981 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    11041 ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    11101 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca
    11161 tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct
    11221 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    11281 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    11341 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    11401 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    11461 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    11521 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    11581 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    11641 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    11701 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    11761 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    11821 ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt
    11881 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    11941 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    12001 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    12061 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    12121 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    12181 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    12241 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    12301 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    12361 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    12421 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    12481 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    12541 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    12601 agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga
    12661 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    12721 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    12781 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    12841 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    12901 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    12961 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    13021 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    13081 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    13141 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    13201 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    13261 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    13321 atagcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    13381 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    13441 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    13501 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    13561 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    13621 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    13681 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    13741 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    13801 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    13861 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    13921 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    13981 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    14041 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    14101 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    14161 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    14221 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    14281 aacaaaatcg atactgccca gataaacaat caggtcagac accaagctgc cgcggatcgc
    14341 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    14401 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    14461 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    14521 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    14581 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    14641 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    14701 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    14761 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    14821 ataggcggca acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc
    14881 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc
    14941 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    15001 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    15061 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    15121 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    15181 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    15241 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    15301 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    15361 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    15421 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    15481 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    15541 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    15601 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    15661 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    15721 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    15781 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    15841 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    15901 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    15961 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    16021 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    16081 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    16141 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    16201 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    16261 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    16321 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    16381 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc
    16441 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    16501 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctggcgcg gcgaagcacg
    16561 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    16621 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    16681 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    16741 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    16801 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    16861 cgtttccggg ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    16921 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    16981 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    17041 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    17101 ggagccgacc gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    17161 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    17221 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    17281 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    17341 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    17401 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    17461 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    17521 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    17581 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    17641 gatctgtgac acgcttttgt tattccatag caaaaacgca gcaacaaatt tacgtatttt
    17701 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    17761 tctctggtga ttgaaaaata aacagttttg tacgttctgc actatccgag gaaggatttt
    17821 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    17881 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    17941 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    18001 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    18061 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    18121 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    18181 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    18241 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    18301 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
    18361 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    18421 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    18481 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    18541 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    18601 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    18661 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    18721 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    18781 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    18841 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    18901 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    18961 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    19021 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    19081 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    19141 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc
    19201 agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt
    19261 gcgaattatc tgatgcaaag ttatgttatg ttatgttatg ttaggcaaag taatcttcta
    19321 ttattaataa gcacatcaaa acctttttga atattaaata ataattaatt agcatcatcc
    19381 tcattcatta attccgctta acaatagttt cacaccttac ccccattgcg acacctcgga
    19441 aatcatcact ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg
    19501 aattcttaaa tgaggcattt tcacacaatt atcttacaga taaaaaaacc atacttgcaa
    19561 ttaagaatta gaacaattca ctatataaca ttacatgtaa agcatataca cctaattatt
    19621 ttgtcattat taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc
    19681 tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca
    19741 gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca
    19801 aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg
    19861 aagaagaagg taacaaggta ctgaaactga acatcggcaa cccagcccca ttcggttttg
    19921 acgcgccaga tgaaatcctc gttgacgtga tacgcaacct gcctaccgct caagggtatt
    19981 gcgattccaa agggctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg
    20041 gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg
    20101 ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct gcaccagatt
    20161 acccactctg gaccgcggcg gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg
    20221 atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta
    20281 cgcgtgggat tgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc
    20341 ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc gccgatgaaa
    20401 tttatgacaa aattctctac gacgatgctg agcatcactc aattgcgccg ctggcacctg
    20461 acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc
    20521 aggggtggat ggtgttgaac gggccgaaaa aacacgccaa aggctacatc gaaggtctgg
    20581 aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg
    20641 cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggcggtcgt ctttatgagc
    20701 agcgtaaccg cgcgtgggaa ctgatcaacg atattccggg cgtttcctgc gtgaaacctc
    20761 gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt catgacgatc
    20821 agaaaatggt gctggatttc ctgttgcagg aaaaagttct gttggtgcaa gggacggcat
    20881 tcaactggcc gtggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg
    20941 agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat
    21001 ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg atgatgtcat
    21061 catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact
    21121 cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt
    21181 gcaggtagcg atggtcgccc atgcgctggc agctatcaaa aatcggaaat ttggcggtaa
    21241 tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcctcag aagtgctcac
    21301 cggcgatctc cctactccgg tgaaatactt caatgcgcaa atcgctcagg agtacaaggc
    21361 tattgaaaaa atcgctcagc agaaactggt cgatatggtt ccggaagaac tgcgggatat
    21421 ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca
    21481 ggcagatgca ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg gaaataatga
    21541 attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca gccaggagat
    21601 ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca
    21661 ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca ggcaataagc gccggatgcg
    21721 acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc
    21781 acgataatgg tgaacggtac ccccaacttc acaaagtcac tgaagctgta atttcccgga
    21841 cctaaaacca gggtgttaac aggtgaagac accggtgtca taaaggcggc ggatgctgcc
    21901 atcgccacga ccatcgcaaa tggataaggc gacactccca tcgttttggc agcagccagc
    21961 gcaatcggag ccatcaacac cgccgtcgcg gtattagaga taaacagccc aataaccgcc
    22021 gacaagacaa acaaacaccc cagcatcata tgtggcccat aaccgccgcc aatatccatc
    22081 agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc aaacggcatc
    22141 atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct ttcagcatct
    22201 atacagcgga atttccccat cagcaggcag gcgatgatag cggcaacagg attaggaatt
    22261 tcatttgtca gcattaacgc caccattagc accagacaga aaatggcatg gggtgcctgg
    22321 ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac gaagtcgcgg
    22381 ccctgtttgg ccagcatacc gatcagtttc cagttaccca caaccaggat gatatcgccc
    22441 agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg cttgagcccc
    22501 accacattca gtccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt
    22561 tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa
    22621 tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg
    22681 acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca
    22741 ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagcg
    22801 ccataacgct cacgtaattt gagatcatcc agccgttgac ctatcattgg cgatccgggg
    22861 cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag
    22921 gttcgacgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag catgaaacgc
    22981 atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta
    23041 aagaaactga agccgtgata gccttcacgc agcaattcac tgttgactac caggttcggc
    23101 ggcgtcgcca ccagcgtcat catgccgcta atcagcccgg caaaacttaa cggcatcatc
    23161 agacgtgacg gcgaagtttg catacgcatg gcaacgctta acaccacggg aataaagata
    23221 gcgacaacgc tggttgaact cataaacgcg ccaagccccg cgacggtcag catcaacaaa
    23281 accaacattt tgatttcact gttgcccgcc actttgacca gccatgttcc cattacggtg
    23341 gcaacaccgg tacggaccaa accatcgcca ataataaaca aggcggcaat caggacaacg
    23401 ttaggatcag aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac
    23461 gcgacaataa caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg
    23521 atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt
    23581 taattgaggg ggatgacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc
    23641 atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt
    23701 gctccagact gtggaggacc aaatcgacct cattcaggcg cggggtatct gccggagcgt
    23761 taaccgcaat gacatggcaa cccgccgcta ggccagaaag cacgccagcg ggagcatctt
    23821 ccaccaccac acactcctgc ggcgcaagcc ccagcagctg cgcacctaac agatacgcat
    23881 caggttctgg ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa
    23941 gccccgctat tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc
    24001 acggaatacc tgctttattc aaatgattga gtaaggcgat tgccccagga agcgcggtaa
    24061 taccttcggt ttccgtggcc tcgatttgct ccaaacgcgt aaactcggcg gcaatatcag
    24121 cctcggattt gcccgccata aaatggcgca aagaggtgat cgcctgttta ccgtgaatga
    24181 aagccagcac ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt
    24241 ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc
    24301 gcacgcgggc ctcctcaggc gttgataatt tgattaattt cgttggcgct caaatggtac
    24361 tgacgcgggc aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc
    24421 tgggcgttaa aaccatgagt cccagcgtca aaatgggtat agcgcccttc cacattaacc
    24481 ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg
    24541 acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca
    24601 tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac
    24661 tcgcgatcca attcacgcat ctgtaatccg taaccacgct caataattgt ttgcaggcga
    24721 cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa
    24781 ataagacgtt gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga
    24841 aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc
    24901 atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg
    24961 gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg
    25021 aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag
    25081 ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc
    25141 aagcagaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa
    25201 acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg
    25261 tttttccagt ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt
    25321 aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa
    25381 aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc
    25441 actatcaggt atcctttagc agcctgaagg cctaaatagt acatattcat tgagtcgtca
    25501 aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat
    25561 ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca
    25621 tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac
    25681 ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc
    25741 catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct
    25801 gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc
    25861 aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc
    25921 agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta
    25981 taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt
    26041 tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc
    26101 acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga
    26161 agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta
    26221 cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa
    26281 accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc
    26341 tatccgcaac ggtaaatgcg ttgacacctc tatgggtctg accccgctgg aaggtctggt
    26401 catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct
    26461 gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct
    26521 gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc
    26581 gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc
    26641 gctgatggat ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc
    26701 gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga
    26761 acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc
    26821 tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac
    26881 tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc
    26941 ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc
    27001 gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt
    27061 ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact
    27121 acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc
    27181 tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca
    27241 aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca
    27301 cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa
    27361 atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa
    27421 ctgacccgca acagcttcgg tggtgccaaa aacaccaaca tcaccggcgt tatcgttaac
    27481 aaactgaacg caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc
    27541 gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc
    27601 ccgctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc
    27661 gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc
    27721 gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc
    27781 ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca
    27841 gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg
    27901 cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac
    27961 accaacacct ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac
    28021 gatcacgaac gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg
    28081 atcgaatctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt
    28141 tatcagctga ctgaactggc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac
    28201 gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta
    28261 ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactaggt
    28321 gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc
    28381 gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac
    28441 gtggtgctcg gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct
    28501 gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc
    28561 agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt
    28621 gactgtgcga tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc
    28681 gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc
    28741 ggtacttctg gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag
    28801 gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct
    28861 gacgttgcga aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgttcatc
    28921 ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg
    28981 atctccatcg ggccgatgct gcagggtatg cgcaaaccgg ttaacgacct gtcccgtggc
    29041 gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag
    29101 cagtaatctc gtcatcatcc acagctttgc gctgcggata tctgaaccgg aaataatcac
    29161 tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact
    29221 gcgctgcatc aatgaattgc gccatctcac tttgcatact taccactttg ttttgtgcaa
    29281 gggaatattt gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg
    29341 cccgatacgt tggtgattat cttttttgtt gctattttaa ccagtcttgc cacctgggta
    29401 gttccggtgg ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa
    29461 acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct
    29521 gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc
    29581 ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt
    29641 atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt
    29701 atcctggcgc ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt
    29761 attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc
    29821 attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg
    29881 acttatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt
    29941 gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg
    30001 tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa
    30061 aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg
    30121 gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt cctgacagcc
    30181 gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc
    30241 agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac
    30301 ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc
    30361 cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggttggtaa tggtgaagca
    30421 ggtaatgcca gcgtgttaaa tactatcctc aacagcattg ccaatgccat tagcggtctg
    30481 gacaacgcgg tcgcggcctg gtttatgttg ctcttccagg ctgtatttaa tttcttcgtg
    30541 acgtccggtt ctggtcaggc ggcattaact atgccgttac tggcaccgct tggcgatctg
    30601 gtcggtgtta accgtcaggt taccgtgctg gcgttccagt ttggtgatgg cttcagccac
    30661 atcatttacc caacctcagc ttcgttaatg gcaacgctcg gtgtgtgccg ggtggacttc
    30721 cgtaactggc tgaaggtggg cgcgaccctg cttggactgc tgtttattat gtccagcgtc
    30781 gtagtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg
    30841 ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc
    30901 cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct
    30961 tccggcgtca gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga
    31021 gagacgcaac tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc
    31081 ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg
    31141 gattccagca gttgctcatc ggcctgaact acaccgcctg cggtcgcatc taacatgccg
    31201 ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc
    31261 acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct
    31321 tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga
    31381 cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg
    31441 tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt
    31501 acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata
    31561 ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg
    31621 gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa
    31681 agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg
    31741 gttatcggga aatgcagcat catttgatcc acttcgctgt cgcagttgcc acgcacagcg
    31801 ataaccttat gtgccacttc attaagccgt tcagcaactt tggctggcgc gtaaccctcc
    31861 ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca
    31921 ccgctttgga caaacaactc cacaacacgt tccgtcgccg gtaacgaccc atgaatgtcc
    31981 gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg
    32041 attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga
    32101 cgctcgctgc cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg
    32161 tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa
    32221 taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact
    32281 ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg
    32341 acgctcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg
    32401 cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc
    32461 ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa
    32521 cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc
    32581 gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga
    32641 taacacataa ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat
    32701 tatgtcctcg gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca
    32761 cgtgacgaat actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa
    32821 acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt
    32881 acgctgtttc tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa
    32941 ggcggtcagt ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt
    33001 gttgatcatt ctcctgccga tggcggcgaa ccgttaagcc tctttgagtc tggtgccatt
    33061 ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc
    33121 gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat
    33181 catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt
    33241 gaaactcagc gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga
    33301 ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc
    33361 ctgcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg
    33421 cgccctgcca ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt
    33481 taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa
    33541 tggcacaacc tgccgctatt attcgtataa agaaccttcg tttgcgtacg tttatcggaa
    33601 ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc
    33661 ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa
    33721 cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc
    33781 aggatgtgct cgatatcgta cgtgaacatc actgggtgac gtatgctgaa gtggagatcg
    33841 ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt
    33901 aatcgccata tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg
    33961 tcgccatttg attccgcgtt tgctggagct gggccatcaa attacggtag tgacgcgtaa
    34021 cccgcagaaa gccagttccg ttctcggccc tcgggtgaca ctatggcaag ggcttgccga
    34081 tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga
    34141 taaacgctgg actcacaagc aaaaagagcg tctctgccaa agccactgga atatcacgca
    34201 aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca tttccggctc
    34261 ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca
    34321 taacgaattt actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag
    34381 tgacaaaacg cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat
    34441 tctcggtaaa atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg
    34501 gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga
    34561 taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt
    34621 tgcccatgcg ctcggtcatg cgctgcatcg cccggctatt ttgcgcgtcc ccacaaccgc
    34681 cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc
    34741 taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc
    34801 ggatgtcgtt cgctgatgtg gtttacagca aacatccgcc agttaactcc ctgtgttaca
    34861 ggattagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa
    34921 ctctctgtct aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt
    34981 gccctgcatt gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc
    35041 ttgtggtgcg cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt
    35101 ttgtaatacg taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag
    35161 cagcgaaaca atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat
    35221 taactccaac agcgccattt tgcggtgttg cataatctcg tcatccggca ccactgtaat
    35281 atccaccaac ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc
    35341 atcaagccag cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg
    35401 gatcaccaat ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga
    35461 ataacgcatc atgcgaaaag ccattaattc ttccggctta ctttggtgct ctatcactac
    35521 ataaatataa cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg
    35581 ccgcaggtct tcatcaataa aactgtttgg ttctagttta agcgtcgtta aatcacacag
    35641 tttgcgcagc ggcgcgggaa gatgaatatt aataaaattc cgcgcggtgt ctggatggcg
    35701 taaaaaagat ttaaataccg catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt
    35761 ccgtcacccc tcaaatttaa gatgacgcga caataaccac gattaacacc caatgcagca
    35821 ggcagttttt atctttggac agcgaattca gaagtatttt tagcggactg caacttttac
    35881 atttcagcgg aagctgactc gcaaattccc cttgaaaata ctccggcagc gttagccacc
    35941 ggagaggaaa ttatttcagc gatcccttaa ggaactgttg cagacgaggg ctttgcgggt
    36001 tgccaaataa ctgctccggc gcgccctctt cttctatttt cccctgatgg aggaaaatga
    36061 cgtgagtaga aacatggcga gcaaagccca tttcgtgagt cactaccacc atggttttcc
    36121 cctcttctgc cagttgctgc ataatacgta acacttcgcc taccagttca ggatcgagcg
    36181 ccgaggtagg ttcatcaaac agtaaaactt ctggttccat cgccagcgcc cgcgcgatag
    36241 aaacacgctg ttgctgaccg ccggaaagat gcaccggata tttcccctgc gcacgttcgt
    36301 ctatcccgac tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc
    36361 ccaacacctg aatcggcgct tccatgacgt tttccaacac cgtcatatgg ctccagagat
    36421 tgaagtgctg gaataccatc gtcaggcgtg tgcgcagtaa gcgcagttga tttttatcgg
    36481 cgactttgag ttgaccgtct ttgtcgcgca ccagattgat cgtctggcca ttgaccacga
    36541 tcgatccttc actcggtttt tcgaggaagt taatgcagcg cagaaaggta cttttccccg
    36601 atcccgacga tccgatgatg cttattacat ctccggcatt cgcttgcagt gagacccctt
    36661 tcagcacttc gtgttcgcca tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg
    36721 acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg tttttccgct
    36781 ctgcgaaaga ggctgatcag gacataagag atgattaaat agagcaccgc ggcaatgccg
    36841 aaggcggtaa aaggttgata cgtggcggcg ttaatatcgc gggctatttt cagcaaatcc
    36901 ggcacagtgg ccgtaaacgc cagcgcagta gagtgcagca tcaggatcac ttcgttgctg
    36961 tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcagcgata cattttaaag
    37021 gtcgagaagc cataggctct ggcggcttca atttccccat gcggaaccga acggattgcc
    37081 ccagcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag cacggtacag
    37141 ttcaggccac tgcggaagaa agcgttaagg aattcggttc ccttaacaat ctcaagcgtg
    37201 tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg aaaaatatag
    37261 gtaaataacc agattggaaa ctggatgtat ttattactgg agacacgacc aatcgccaga
    37321 aacagcgcca ggactccgcc tatcactacc gacaaaataa gcagccacag agtgatcgcc
    37381 acaccagtaa agcgataacc gtcggtccac agcagcggtt tccagtattc atgtaagatt
    37441 tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg aacagcagca
    37501 caccattgga aacggtggtg aaaaccaggt aaatcacgcc acagacgatg gcgaaataga
    37561 acggttccca ggtacttttg cctgccagct gcgtggcttt gaccacatct tccaggccga
    37621 gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca atgcctggca
    37681 gggcgtaacg catcatcgcc ggaaacatga tcctccgaaa cacttgcccg cgagtaaaac
    37741 cgaacgccgt cgccgcctct atatgccctt tcggcactgc cataaacgcg ccacggaacg
    37801 tttcggtaaa ataagcaccg tagataaaac cgagagtgat aataccagcg accatcggat
    37861 cgatatcaat ctgcccgacg cccatcgcct ccgtcaccat gtttagcgca atctgcaaac
    37921 catagaaaat cagcagcatc aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc
    37981 cttcgaaaat cagccccaaa agccgatttt gcgagagctt accgccagcg ccaattaaac
    38041 cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg agcgcaccct
    38101 gtaaaataac acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt
    38161 accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca ccgatggcga
    38221 ttaaccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt aagtaccgtc
    38281 agcgcgcatt tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat cttctttacg
    38341 caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg gaccaccgaa
    38401 tttgtaatct ttaccgacgg gttgtttgag gaaaccttcg ctggcagcga cctcatcctg
    38461 gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct gcccctgata
    38521 cgagacgatt tcaatgcctt ttggtgccca gtgttcatta ccgaacgtct cctgggtggt
    38581 gccctgcaat acgcctaccc gtttgccttt cagcgactcg actgtcggct gaatgtcaga
    38641 atttttcgcc accaccagac gagaatcggc agcgtacagt ttgtcggtga aggcaatttc
    38701 ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt
    38761 taaggacggg attaacgcat ccagcgggtt ttcgacaaac gtacattgcg tattgatgcg
    38821 cttgcacaac tccttcgcca gctcgatatc gaaaccaacc agttcgcctt gtgaattttt
    38881 tgattcaaat ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg gaattgcagc
    38941 aagtgccgca gttgcgctgg agaaggccag aaccagagag agcgatagca ccagtttttt
    39001 cataatgtcc tcaactgaca gtctttttat agggaatatt tacaggtttg ttacacttat
    39061 cgtgccataa aattgaccat caaggcaaaa tatcccgccc cgttagacgt attatttttc
    39121 attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa cctggcgtgc
    39181 accatgatga tcattccatc aggtacagct tcccagcgac gtatcagtca ccgtagacat
    39241 taaagtcgaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa
    39301 gcgccttatt gaaggcagcc gtcagttcag catcatcttt acgtagccct acaccggtgc
    39361 catcaccgaa gtattttttg tcttttactg atgagccagc aaaggcgaaa tctttaccag
    39421 caggttgctt gaggaatcct tcgctggcag caacttcatc ttgtaacgca gcatccagac
    39481 gtcctgcagc cagatcggaa tagaccaaat cctggttggc ataggccacc acatccacgc
    39541 ctttactacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc agcacaccaa
    39601 catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg gcagcaatca
    39661 aacgagaatc tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg
    39721 taatggaaag cgacgaaata atagcgtcga tttttttcgc tttcagtgag gggagcagcg
    39781 cgtcaaagtc actggcaacc caggtacatt tcacctgcat ccgtttgcac atctcgttac
    39841 cgagatcgat atcaaagcca acaaaatcac ctttagcatc tttcgacgag aacggcgcat
    39901 aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg
    39961 cggagagacc gactaataaa gacagagcga gaatcgactt cttcatacat aaccctcaag
    40021 tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg
    40081 tcttatgcca tcttgacgac aacagtaaca ttcaacgtta aatatgttaa taagacgttg
    40141 cattattgtc ctgaagttga agatagcagg tatagcggtt gaatcgcagc gtttcgattg
    40201 aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc tgaaacaggg
    40261 caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag ggttattgca
    40321 aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga ttgcgggcga
    40381 tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc ctgagtcatt
    40441 aaacgcagat ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc
    40501 tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct atggacaatg
    40561 ccggacaggg ttttgattga acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg
    40621 gaagagatgc tggcggaaat atcgcgcgca tcgagcgtga catcagctaa tgcctgcact
    40681 tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg gctcatcacc
    40741 agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataagcgcac gccataaatc
    40801 gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg ccctgtcaga
    40861 cttgcaggca gactttgcag tatttcggcg ggagttgcaa gtcagggtgc cagaccggca
    40921 ccctcagcga aggcatcatc cttcgttatg catttcgaga ttttcctctt cgttctgacg
    40981 ttgcactgct ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt agccctgatc
    41041 aacatctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct ggatatccgg
    41101 attttcagcg cgaacggcgt cgatcagatc gttcagatcc tggaaaatca acccgtcagc
    41161 accgatgatc tggcgaattt catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct
    41221 cggcatatca ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta
    41281 cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag tggtgccacg
    41341 gacgatggag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt
    41401 cagtttacgg cgcacggact tacgacgcag ctgctggccc ggcatgataa aggtgcggcc
    41461 aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac gagcaatttc
    41521 cagcgcgata tcacacgagg tttctgggat cgggatcacc acgtcgatat ccagatcttc
    41581 ccattcgcgg gcaattttct cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac
    41641 ggaaattttg tcgataaacg agtccgggcg ggcaaagtat acatactcaa acaggcacgg
    41701 attgctgacc ggattgtcag cacattgacg ggtaaacaac tgcccttctt cagtgatgta
    41761 aatcgcttcg cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac
    41821 gctttcggaa gcgaccatat attctgtacg gttctcgtca atatcacgtt ttcccagtac
    41881 cagcggacga atcccgtttg gatcgcggaa agcaaccata ccgtggccga taatcatcgc
    41941 cacacaggca tacgcgccgc ggattaagcg gtttgtggca gcaatggcag cgaaaatatt
    42001 gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctggcgaaga tattaagcag
    42061 aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg
    42121 caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat acggggagtt
    42181 aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt aacgcacatg
    42241 accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata catcgctcac
    42301 cagcccgttc gctttacgca aacggaagca gttattggca tctatggtga tgatgccggc
    42361 ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact ggttaaccgg
    42421 cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt aagccacatc
    42481 tcagagcact tacgctctgg gcaagaaact tgacgagctt tgcagataat caaaaaacca
    42541 tctgatgata aaactgaatt gcgggatcag ctgtgatttg ctccagtctt cacttttcga
    42601 caccccggta aaggagtcga gaaagaagag aatggcggca acaatcaaca caccgcgcaa
    42661 cgcaccgaaa cagacgccca gcacccgatc ggtgcctgac aaccccgttt tctccaccaa
    42721 ctggcctatc acgaagttca cgatagcacc aacgatcagg gtagcgataa acagtaccgc
    42781 gatggcaatc ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta
    42841 agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg ataacgcttc
    42901 acgaacaaag ccgcggatca ggctaaccag agaggaaaaa gcaatcaccg cgattatggc
    42961 gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct gttcggggcg
    43021 cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa
    43081 tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca tcgtgcaatg
    43141 cgtgtcggat gcggcgtaaa cgccttatcc gacctacggt tctaacactg cccaagcctg
    43201 ataagacgcg ccagcgtcgc atcaggcaag actgtgttaa ttcggcgtat agcccattac
    43261 cacgccactt aagccagaaa gttgcttcaa ctcacccagc gaacctttca gcttatcttt
    43321 cgaggcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg tggatggcga
    43381 cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca ctttatcggc
    43441 atttttcagc gcacccagtt gcacaacata ggctttaccc gttggcgctg ctttttcttc
    43501 cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaacctttg gtttaggcgg
    43561 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    43621 attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc
    43681 ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc
    43741 aggctcatca cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata
    43801 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    43861 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    43921 cttccatgac atgtgcgacc gtgtggaaag aaccacacac cagcacggtg tcttccgctt
    43981 tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat ttgccgttac
    44041 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    44101 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    44161 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga
    44221 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    44281 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    44341 tgacttccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    44401 caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    44461 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg
    44521 ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    44581 ggaagatgcc tgctttctcg cgaccaatac tttcgcgatc tggacccagc cagtcggtat
    44641 gatccagcgc aatactggtt actaccgcgg catcggcatc gacaatattg gttgcgtcca
    44701 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc
    44761 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    44821 caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcccggccc tgcacacgta
    44881 cgcgctcggt ataacgcacc agatgaggcg aactgtagac gcccactttg taccctgccg
    44941 ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta cccgcaacgg
    45001 taaacacaaa tggcgctggt ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct
    45061 caaggccgag ctcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    45121 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    45181 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    45241 cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    45301 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    45361 cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat ctaggcggca
    45421 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttag
    45481 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg
    45541 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agtgccgcag
    45601 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    45661 cggagaagca gatcagcggg cagttatctt cctgcgcctg ctcaacggca cgcacgaaac
    45721 gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat gccgcagcga
    45781 caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    45841 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagctcat
    45901 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    45961 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    46021 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    46081 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    46141 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    46201 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    46261 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    46321 gcgtttgtga cggattattt cgatgacgcc cggcaaaata gaaaccacaa taatcccgac
    46381 gatcagcagc ttaaggttat cctgaaccat cgggattgta ccgaagaaat agcctgcgta
    46441 ggtaaaaagc agtacccaca acagtgcgcc gatcacgtta taagcagcga aatgacggta
    46501 cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg
    46561 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt tgtcgagata
    46621 actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga acaaccgccc
    46681 gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca ctaccatcat
    46741 atggacgttg agatcgttgg tttccagtga tgccaacgct ccggcaacaa acagcaatga
    46801 atcacccggt aaaaacggca ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    46861 aatggcataa acccagacgc cgtactccgc gactagctct gccagatgca cgtcaatgtg
    46921 caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct tgtactcgtc
    46981 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattctcgtt
    47041 agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat
    47101 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    47161 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    47221 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatcaacca
    47281 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    47341 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    47401 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    47461 ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa
    47521 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    47581 aattcgcatt tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat
    47641 gcaccacttg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag aagacggtga
    47701 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt
    47761 cattctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc agcgcaattt
    47821 tataaactgg cagttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    47881 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagag
    47941 ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt
    48001 acccgaagca tcccctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc
    48061 gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt
    48121 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    48181 cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    48241 accttcgcta tccggcaaca acggcagcat attgaacgcc agttgacgac cgaagaaatc
    48301 ttcttcgtca atcggaatgc cgttgagcaa tttcgcactc tgccccgcta acgcatcgac
    48361 cgcttttttg ccctgggcgg aggctgaaat caggctggta acactgatac gcgacaaacc
    48421 gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac tgtctggtac
    48481 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcaccac
    48541 cagcggtacg tcgggttcga gagcaaacag tccgctgctg tcgatcacca ggcaacctga
    48601 gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag
    48661 ctgcgcctgc gtccagtcga attcagcggc atcctgcacg gtgattgttt taccaccaaa
    48721 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    48781 ctgacgttca gccagcgttt caagcagggc ttcgcccata gcgccagttg cgcccaggac
    48841 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    48901 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    48961 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    49021 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    49081 actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    49141 agcgtcggtt gatcgagcgg accatgcagc gtaatgcggc caaactcagg cgcgggcagc
    49201 aacgtatcca gcgcaacgtg ttgttcatgc ccaataaact tgctataggc ttcaaacact
    49261 tgcgtcgtgc cacgcgcttt accctccagg gtatagcctg cgatatgcgg cgtgccgata
    49321 tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac atccagcacc
    49381 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    49441 ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag tttttcatcc
    49501 gccagatgta gcgttttgta cggaccatct ttaaagagtg gcgtatggaa agtcagaata
    49561 tccgcgcgct ggactaactc atccagcgag cggaaatcac cctcatcccc acggtcggcg
    49621 cgaggcggat cgcaaagtaa ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga
    49681 cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga aaatccatcg
    49741 cgttcggcaa gcatcagcag ggaggagaaa acatattcca ccaccgcaat cgcattacag
    49801 ccaggtgcag cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc gacatggtcg
    49861 gtccccgcag tggcagttcc aacaaattta atgggttttc ctgccagcaa agattcattc
    49921 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc gacggggatt
    49981 ggacgcccgg gaaccgcgat cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    50041 atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg
    50101 gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata
    50161 cgtaatgata caacctattt ccggccctcc tcctgggcag ccaccaggtc agggagataa
    50221 cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag
    50281 cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt gggcgggtat
    50341 caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc
    50401 tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct
    50461 tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg tcacggatta
    50521 tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac
    50581 cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc agcgcgaggc
    50641 gaccgaccgt cctttattac cggcggagca caatgcgcta aaacagctgg tgaccaaact
    50701 tgcggcggca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg aactttccgg
    50761 ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggctaca
    50821 ggcgcgtcag acgctaagcc agcaaaatac gccgacgctg gaatcactac agatggcgct
    50881 aaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata
    50941 tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct
    51001 ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc ttaatccttt
    51061 tccaccgatg atggacacgt tgcaaaatat gccaacgcgt cccgcgctgt ggatactgtt
    51121 agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa atgacagtat
    51181 cgtgacaata atacccagca ccgcagagat cgccccggca agaaataccg aagagtaacc
    51241 aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga tatcctgaaa
    51301 cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt taaccacctc
    51361 cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa
    51421 tgcgacccat gcacctgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag
    51481 agagacaatc gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg
    51541 catcacgaca aatgcgccgc caaacgcggt aagagtaaag cccgccatcg cccatccttt
    51601 gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc
    51661 cagccctaac cctggtttcc agataagccc gacaacgctc cacagcgatg gacgttctcc
    51721 cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatac
    51781 cattgtggtg atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg
    51841 cgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatca ctttgccaga
    51901 gtgttttggc cctacgatgc ctaaccccca ggtcagagcg cctgtcagta actggctttc
    51961 accaaaccca agaatcaaac gcccgatgac caacagggca aatttgaacg gtgcggagac
    52021 aggcaaaatc gccgccagca gcaacgcgcc gccagccaga ccacaagcta acatcccctg
    52081 aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac cacgcgtcag
    52141 caccgtagcc agaaactgaa tcccgacggc aatgccgacc atggtattgc catagcccag
    52201 atcatgatga acaaacagcg ggataaccgg caacggcaac cctacggtca tgtaggtgag
    52261 aaaaaccgca aaagcgatgc ggaagagcga aaaactggca gaagatcgtg tttcggtttg
    52321 gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc
    52381 atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga
    52441 aggaaatcga caaagcggga agtttgccta gaactggtgg cgattgtcaa tgatgtgaaa
    52501 aagggaacca tcaggttccc ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc
    52561 ggatgcgacg ctggcgcgtc ttatccgacc tactccggcc tactgcgaat taatctttca
    52621 gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataacag
    52681 tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acccgcagcc tgctcgtcca
    52741 gctcttcaat gttgatgctc ggggcgataa agccgtgttc cagcatcagc agagagtaga
    52801 tagcttcctg cacacccgca gcgcccagtg agtgaccggt catggctttg gttgcagaaa
    52861 tcgccgggct cttatcgccg aacacttcac ggatagctgc cagctctttc acgtcgccaa
    52921 ccggagtcga agtaccgtga gagttcaggt aatcgattgg ggtatcaacg ccatgcatcg
    52981 ccatctgcat acaacgtact gcgccttcgc cagacggagc aaccatgtct gcaccatcag
    53041 aggttgcgcc gtagccaacg atttcagcat agatgtaagc accacgcgcc agcgcgtgtt
    53101 ccagctcttc aaccactacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag
    53161 cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac agcgcaccca
    53221 tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc gccgccgcca gcaaacacga
    53281 tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg
    53341 cacacgcgga gctgatggag tagttaacgc catgaatttt aaacggcgtg gcgagacagg
    53401 cagaaacgcc ggatgccatc gctttagtga ccacatacgg gccaaccgct ttcaggccgc
    53461 gcgggccgcg cattgcgtca gcgccgaaca cctggaaacg cggggaaccg ccgccggaac
    53521 ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggagagagg cccgcatcag
    53581 cgattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa
    53641 ctttgcggtc aatgaggcca gtgatatcca gttttatgtt gccccagacg tggctacgca
    53701 tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg
    53761 ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg
    53821 cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc
    53881 gtacacttgt acgccgaaca agtccgatca gccatttaat agagaaattt gcgcagcctt
    53941 acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac
    54001 tccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt
    54061 gacgatgtct atttttccaa cgataacggg ctggaagaga cgcgttatgt ttttctggga
    54121 ggtaaccaat tagaggtacg ctttcctgag catccacatc ctctgtttgt ggtagcagag
    54181 agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttga tcagtttcgc
    54241 gaagcgcatc cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc
    54301 ctcacccgtg cggatttagc cttagcgcat caacactggc cggaactggc tccgtgggca
    54361 gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat
    54421 gaaggccgcg tgacgctgga tttatggttt ggcgatatta acgaactgac cagccaactg
    54481 gacgattcgc taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagctaaa
    54541 aacccggata tgtggacgca aaatctgttt aacgccatgg caaggttggc gcgtccaggc
    54601 ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggaagccgga
    54661 ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg tggggtgatg
    54721 gaacagacat taccactccc ctgctccacg ccgtggttta atcgtacggg cagcagcaaa
    54781 cgggaagtag cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct ggcgttatta
    54841 cggcgcggct ggcaggtagc gctttattgc gcggatgaag cccccgcgct gggtgcttcc
    54901 ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc
    54961 tttttctcta atgcgtttac ttttgctcgt cggttttacg accaattacc cgttaaattt
    55021 gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc
    55081 gcacagatgt tgtcaatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg
    55141 gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt
    55201 tggctgtgcc cagcagaact gacccgtaat gtgctggaac tggcgcaaca gcagggtttg
    55261 cagatttatt atcaatatca gttacagaat ttatcccgta aggatgactg ttggttgttg
    55321 aattttgcag gagatcagca agcaacacac agcgtagtgg tactggcgaa cgggcatcaa
    55381 atcagccgtt tcagccaaac gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc
    55441 catattccga caacgccgga actggcagag ctgaagcagg tgctgtgcta tgacggttat
    55501 ctcacgccac aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc
    55561 agcgaagata cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat
    55621 tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc
    55681 ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag gcaatgttcc cgattatgag
    55741 gcaacactcg tggaatatgc gtcgttggcg gagcaaaaag atgaggcggt aagcgcgccg
    55801 gtttttgacg atctctttat gtttgcggct ttaggttctc gcggtttgtg ttctgccccg
    55861 ctgtgtgccg agattctggc ggcacagatg agcgacgaac cgattccgat ggatgccagt
    55921 acgctggcgg cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg
    55981 gttaaggcgc ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc
    56041 aacctacgtg tgacgtgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc
    56101 tccagacgcc gcaacttaca gctgtgacgc ctgctgaaac aggttttccc acatatcggt
    56161 caccagcgcc tggtcacgcg gcgacaattc accggcacca atggcttttt ccagactctg
    56221 gctaacggtc gtatgcaccg cctgagcgga gtggtcatca ccactttcca gttctgcgat
    56281 ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg
    56341 gtcaaccatg ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt
    56401 tcctcattgt aaaacgtggc gctggcttca gttgagcgct tccggccacg ggaactgttc
    56461 cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccgggcg
    56521 ttccggaatg ccatttgtca gataatccat cacctgcgca tgtacccgcc gttgaaacac
    56581 cacacgatcc ggttcgaaat ccccttccag attgtcgcaa ctgacgttaa acggatatcc
    56641 agccgccacg cagaataacc agtcgaacgc ctgcggcttc acttcaacat cttcaaactg
    56701 gctttgcgtc tgggcatcgc gtccgtccgg gcaataccag tagccgaaat caaccagctc
    56761 acggcgcgct tttccggcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa
    56821 gccatgagca aagacgattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg
    56881 ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat
    56941 ttcaatcaac tgctcgtagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg
    57001 atctcctgtc cgtggctatc gtaaagtaat ttggcactca tcaccgccga gacgataaca
    57061 atcatcgggc ggatcagctt ttgtcctttg ctcaacacca accgtgaacc catgcgcgcg
    57121 ccgaggaact gccccaccag catgacaaag cccgtcgccc aaatcacttt gccgccgaga
    57181 ataaacagca gcagaccgcc aatgtttgac gttgcgttga gtaatttggc gtgagccgtg
    57241 gctttggcga ggttgaatcc gcacagcata acgaaggcca gcgcgtaaaa cgatccggca
    57301 gccgggccaa agaatccgtc ataaaaaccg acgcagccac cggcaattaa cgcgaagggt
    57361 aaaccgtaca tccggcgctg gcgatcttct tcacccagct ttggcatcag caagaaatag
    57421 aggccgatac aaatcaccag aatgggcaaa atctgccgta agacatcagc ctgaacgtac
    57481 tgtaccaaca gtgcgccgct cattgagccg acaaaggtca tggcaatatt gagtttctga
    57541 tcgcttaaac taaccacttt gcggcgaata aagtagatag tggcggaaat agagccgccg
    57601 caggcttgca gtttattggt tgccagcgcg ttagcgggag acattcccgc agccatcaat
    57661 gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg
    57721 gcgacaaaaa agagaactcc cagcaacagc ggagaaacca taaacaggct attaaacgtt
    57781 tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg
    57841 tcttcttctc aggctttgtt gttcccggtt ttggaggttc aaaccagctt tgcagttctg
    57901 ccccgcaacc atcgcctgat ggcggtaaag gttgatcttc acactccaga ctatcggcag
    57961 ggcaacgtaa tcgtacatgc atatgcgcgc gatgctggaa ccagggtcgc actttgcgca
    58021 accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa
    58081 aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat caagctgaaa atttctggct
    58141 tccacagcgt ggagacaacg tgtttaccgt cgggggaaac taagtccagt gcttgcgggc
    58201 gcaagagctg cgcggaggtc cagcgagttt tcggcagttg cagaaagata tcgacatcca
    58261 gtccggtctg gtggctggca tgaccgccgt tgaaacgccc accagcgggc atccccatat
    58321 cgccaatcag caccgtaccc atgcccagat tgctcacctg gctactcaga cgctggataa
    58381 acatcaccag atccgggtga ccgaaatagc gacgctgatc ggtacgcatg acctgataat
    58441 gttcggactg tatcggcaac gtgtcagcgc cgacaataca gccattagaa aaactgccta
    58501 tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc
    58561 tagcgctact ggcaagcaga gccagcagcg caatcgcggt tttgttcatt ttttaccagc
    58621 gtggaatatc agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg tgatccatta
    58681 aaacgatcgc cagcatcgct tcagcgatcg gcactgcacg gatcccgaca cagggatcgt
    58741 gacggccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatagtg cgccccggca
    58801 cggtaatgct ggaggtcggt ttcagcgcca tatgagcaat gatttgctgc ccgctgctga
    58861 taccgccgaa aatgccgccc gcatggttgc tctggaaacc ctctttggtg atttcgtcgc
    58921 ggttctggct gccgcgcagc gccactacgt caaaaccgtc gccaatttcc acgcctttca
    58981 ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc caggcggtca aagaccggct
    59041 cgccaagtcc ggcggggaca ccactggcaa caacggtgac tttcgcgccg atggagtcgc
    59101 cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcg gggtccgggc
    59161 agaagaacgg attttgttca accagcgacc agtctttgat ttccagcgga atgtcgccca
    59221 tctgggtcag gcagccgcgg atttcaatac caaatttctc ggcgagatat tttttggcaa
    59281 tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga agaacgtcca ccgccgcgat
    59341 aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc atggcctgga cggaagacgt
    59401 ctttaattgc gctgtaatcc tgagaacgct ggtcggtatt ttcgatcaac aagccaatgc
    59461 tggtgccagt agtaacgcct tcaaaaacac cggagagtat tttgacctga tccggctcgc
    59521 ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg atcaaggtca tgttgcaggt
    59581 ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg agcgccagcc
    59641 cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt ccagccatca
    59701 cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc gaaatgttct
    59761 cgtgcggcaa gaagctgctc tttagtgagc ataaacacac catcgccgcc gttatcaaac
    59821 tccagccagg tgaacggaac atcgggatat tgttccataa gatgtaccat gctgttgccc
    59881 acttcacaaa tcaacacgcc atcatcagca aggtaatctg ccgcgttacc gagaatgcga
    59941 cgcgtcagtt tcaggccgtc ggtgccagat gccaggccca gttccggctc gtgacggtat
    60001 tcgtttggca ggtcggacat atcttccgcg tcgacatacg gcgggttagt gacaatcaga
    60061 tcgtattgca ctttcggcaa atcacggaac agatcggaac gaatcggaat gacgttgtgg
    60121 atcagaccgt gttcttcgat gttctgttcg gcaaccgcca gcgcgtcggg agagatatct
    60181 accgcgtcga cttccgcttc cgggaaggca taagcacagg caatggcgat gcagccgcta
    60241 ccggtacaca tatctaaaat atgctgcggt tgcttgctga tgagtccggc aaatttattg
    60301 ttgatcagtt caccaatcgg cgagcgcggc accagcacgc gttcatcgac gtaaaattca
    60361 tgaccgcaga accaggcttt gttggtcagg taagccaccg gaatgcgctc gttgacgcgg
    60421 cggatcacgc gttcaacaat acggtgtttt tcgctggagg tcagacgcgc ggtgcgcata
    60481 tcttccggga tatccagcgg caggtagagc gatggtaaca ccagctgtac ggcttcatcc
    60541 cacgggttat cggtgccgtg accgtaccag atatttgccg cgctgaagcg gcttaccgac
    60601 cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg cttcatcaac gaaaatttta
    60661 tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg ccatgaagat
    60721 gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt
    60781 gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacagataa atgaaaaaga
    60841 aaacaacact tagcgaggag gaccaggctc tgtttcgcca gttgatggcg gggactcgca
    60901 agattaagca ggacacgatt gtccaccgac cgcagcgtaa aaaaatcagc gaagtgccgg
    60961 tgaaacgctt gatccaggag caggctgatg ccagccatta tttctccgat gagtttcagc
    61021 cgttattaaa taccgaaggt ccggtgaaat atgttcgccc ggatgtcagc cattttgagg
    61081 cgaagaaact gcgccgcggc gattattcac cggaattgtt tttggattta cacggtctga
    61141 cgcagctgca ggccaagcag gaactgggcg cgttgattgc cgcctgtcgc cgcgaacatg
    61201 tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat tttgaagcaa caaacaccgc
    61261 tgtggctggc acaacatccg catgtgatgg catttcacca ggcaccgaaa gagtacggcg
    61321 gtgatgcggc tttgttggtg ttgattgaag tggaagagtg gttgccgccg gagttgccct
    61381 gaatgctggc agcaaatgcc ggagcagcgt aaacgcctta tccggcctac aaatatgtga
    61441 taattcaata aattgcaatt cacttcgtag gcctgataag acgcatcaga gcaatactac
    61501 gaacttcagt tcattcaata catactgaaa acgacacgtc gaatcaatcg gttgcccact
    61561 actggtattc acattcacca caatatcgtg tagtacttta tccgttaccg gatgacgagt
    61621 tggcgtaacc gttaccgttg atttatcaca accgtaagag gtaaaggtta acaattctga
    61681 taccgcgcgg cagtatgctc ctctgccatc attatcagaa aaattactgc catcacagag
    61741 tctgccgcct tccatcaggt aggtcgaaag cgtggtggtc actgttgacc ccgtggactg
    61801 aattcgtaac gccttattat ggctgacata attttcactc caggagatat cgtatgaagc
    61861 gaccggtatc gttgggcata ttgtgttatg cactgtaggg gccgtatagc ttccaaatcc
    61921 gggtaaatct gtataggaat aaaaggatgc gatccaaggg caaccataat catcagatgc
    61981 aacggtaatt ttaaacgtca tatatgcaca acgaaaaatt ttcgtatcta tgccagggca
    62041 atttgagtta ttaattgtat caaagtcagt cagtaccaaa gttggagctg aacccgaccc
    62101 agaccaggaa ataacccgca acgtatcgcc tttatacgca aaagaatcat atttcccata
    62161 tgcaacctcc tttggcgtta tcaccgtaac attgaaaaca ctgctcgcta cagcaccggt
    62221 taccttgtcc atggcataat aaactctcac actgtcaaaa ctcgcactca taagtttatc
    62281 tccagcataa ctcttagatg cgccgacaac actcagaacg agcagcatta ggctcgcgaa
    62341 aattatggtc cattttttca tttcagaaat actcaaaggt tgccgtcact gtggcactaa
    62401 agtctccgga cgttacatct cgcccgctct ttgcctgtaa ccaggtatta aaatttaggc
    62461 tgttatttcc ctgattcagc gggagcgtga cgcctgttgt attgttgatt ggcaccgatg
    62521 tgcctgccgc agtttcaata ccaatcccag cgccggatgc tgatgacgag gagtcaaagg
    62581 ccagaaagcc gggtagagca ctatcttctg ttcctgtcaa cgttactttg acattaaaca
    62641 atgtagaact atgacaatct ttcaattgaa acaccaatgg cactcgctct gactgcccaa
    62701 ggttcattaa atcgctggcg gcaatggcag gaaaatgtac ttctgccaac tggctaccat
    62761 taatcactgg cgtacacgat ttactgatta aattgccggt aaagtgcaaa ttatctcctg
    62821 ctattgtggt tccactgccc agcaacatca ggctactaat aaaaaacaac gtacgctttt
    62881 tcttcattgg taatctacct gtaacgtggc ccaggcctca aagtccgcct ctggcagaat
    62941 gacaccactc ttttttactg gcacagccgt taacaccggt aaatcggttt cattcaccac
    63001 aagtggcgta ttgatcgtaa aactctgccc tgcctgtttg agctgaatgc ctaaaccagt
    63061 gatattgctg cttactgcag caatatcaaa atcgcttggt gtgccactcc agctcaaggt
    63121 catttccatt aatgggtcgc gaaccgccgt atcgcaggta atggaatacg gcacggtttg
    63181 ctcataattg ctgccattaa tgtcatcaat aagcacatcc tgaaaagtta tttcaatggt
    63241 ttgagcgttg ctaatcgtgc agttaggcgg ctgcactaaa taaccgtgaa aagagacgtt
    63301 tgagagtgcc atcgagcaaa agccccacag caaaataagc gagatccgtt tcattggtaa
    63361 tccaccacaa ttgtggcact ggcgttaaat tcagctgccg ctaacgttgt accactctgt
    63421 ttcaccggca cagcttccag aacgggaact cccgaggaaa aattaaatgg caaccatgag
    63481 ccagaggtta agtcgagtat tgtgtgatca ctacttttct gaacccgaat accaaaccca
    63541 ggaatacccg tgctgataac tgtttcaccg ttaatcacga ccgttgtcgc ctgcatctgt
    63601 agacgcaggt catccattgc cagattgtta caaacaagcg agtagtcaat gggacgttta
    63661 taatcaaccc tattgatctt attaatgaat acattaccaa actcaactgc cgcaccattt
    63721 accgtacaag gaggaggggc atcgacaagc acccgtaaat tcatacttac tgacttactc
    63781 gctgcaactg caaagccgct accaacacaa catatcagta acccagttac cgcccagaca
    63841 gaacaagatc ctttcatact ggctctcccg gcctcagcct ttgcggctct gctcttcatc
    63901 gacctagcaa ctgttaccgt tgcaagtgaa aaagagcggc atccgagcgc catagtcgtt
    63961 gacataggtc agtaccggca cgctacccat cttcacattc agcggtgcat aggttttcgg
    64021 cgacatcact attggcgaga aaccggctgc cggattgcca tttttctgtg tgctggcatt
    64081 actgaaaatg acgtaatacg acgtcgggtt attgacggta aattcctgac cgttgcgggt
    64141 taaagtaacc ttatattgct aggggttttt catgctgacg ttctccagcg ccttcggtcg
    64201 ccagaacaac ttaatacgcg tttgtagtgc tatttgcagc gtgttggctt tgtttgattt
    64261 tggcgggatc tcacgcacgt tgaagtagaa caggctttca cgatcggcag gcaatttgtt
    64321 gatatccggc attccctgca cttttacctg cccgttcatc attgaatcga tgcgttgcac
    64381 cggtggcaga acagttaacg gtgaactgat cttgttaccc ttttcatctt cgatccagct
    64441 ttgggccaga taaggcagtt tcggatcgtt attgcgcaac gtcacgctga ttgatttgtc
    64501 gctttcatta aatatcaaac gggtacgatc tggcgttaca gaagcctggg cagacaaact
    64561 ggcagcgcta agcagtaacg ctagcgtcgt agcacccaat ttggcagagc aaagtaaatc
    64621 agacatggat cattccttaa tcacgttatt ggttagctga aacagaaaca gggggattgg
    64681 tgggtgtcac ctgttgcgtc tgctcctgaa tgacaggctt tatgtcatca ggaacaaccg
    64741 gcgcgacgtt gcctttatgc tggcatggca gtaacaagcc gttaaacaga tcggcaggca
    64801 gcgggtccgg caggttgata tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt
    64861 cgccaggttt caccccagcc agataaacat tgccgtcatc atcgacaagg cccactgtct
    64921 gctcgttatc attttttact tctgcgccaa acggaggatg gctgccgtct tgtaaacgca
    64981 gcaccgccat cgctttttga ccactgatga cggcaaattt gcggtagcca atcgcccctt
    65041 cagttagcgt ggcttgtacc accgactggg ttgcttcggc gttttccggc agtttgttga
    65101 ggtcgatata cgcctgattg cggtaatagt tattgacgtc agaaacgacg gctttaccaa
    65161 acatattggt ataaacagca gcaccgttac cttctaccgg aacatcggca acgccatcgg
    65221 catcaatcaa caagcgtgtc ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg
    65281 cagtaagcgt tgcgccgccc tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt
    65341 agttcgcact gaggtcaacc tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc
    65401 tggtgtgttt gtcactggtg ccgacgttca actgatagtg agtcgcgtca tcgacacggc
    65461 tgaaataacc gacctgactg ctgtccgtcc cactgccata gtaccgttat agctaacggt
    65521 actgttgtcg ccccacggca tactgagcga aatgtacatg cctttgtcgg cctggttgtc
    65581 atactcgtaa cggtagccag tcatcgagat gctgacatta cgaatactgc ccatattgaa
    65641 atagtgcgag agcatgatgt tgtagtttgt ctgctcctcg cgatcccagt aggtatggcg
    65701 ggtatagttg agataaaccg aaacacccgc atcgcggaag ttctggttat aagtggcggt
    65761 gtacatctct ttgtcgttgc ccgtgcgtac cattccgctg tcactggcat ccagatactc
    65821 gctcatggtc ataaagttct cttccgagaa gcgatatcca gcaaaagtaa cgcggctgtt
    65881 gagctggtca aagtctttgg aataactcac acggaaggag ttaccgtcca gcgaaccttt
    65941 gccataagcg gtgtctttat ccagtttggt gtgcgagtga gtaacatcaa acgcgaccgc
    66001 gccgaatgta gacaaatcgc gaccgacacc aagcgccgca gactgatagt tttcatctcc
    66061 cagtgcgccg ccatataacg accagccgtt agcgatcccc caggaagctt cagcaccaga
    66121 aaaaaattca ccctcgacat ggtgtcccca ctcttgcgga cggcccatca tgatcttata
    66181 gcgaacctga cctggacgag tgaggtatgg catcgaggcg gtgctgatgt catattcctg
    66241 cacctggccg ttctgttctt caatgcgaat atgcaacgta ccggagacag aatcaccaag
    66301 atcctgaata cgaaacggtc cagccggaac ctgcgtttcg taaatcacac gccccatctg
    66361 gctgacggtc acttttgctg tggtgtgtgc cacgccggaa atgtctggcg cgtagccgcg
    66421 cagattggga ggcaacattt gatcgtcagt actgacactg ccaccaacat agttaaaacc
    66481 atcaaaaata tcggatctga ggtaatcctc gcccagcgcc agtttggctt ttaatgatgg
    66541 taacgcccgc ctggcatagt agcgactcca ctcccatttt ttttgagttt catcgccgct
    66601 gaattcatcg tcatcattac tgcgagtatg ttgatagtta gtctgccagt cagcacgcat
    66661 acgccacggc cctaggttaa ccccaaccgt cccgttgccg ctgatctcgt tactatcatc
    66721 accgccattt tcttcgtgcc gggtttgtgc gttgatgctg tagtccgcga cgatcccgga
    66781 gatgccgtca tcccaacgtg aaggcggatc ccagtcggga taagtatatt cgaggtaagc
    66841 ctgtggcagt gaaatgacta atgcggactg gcttaaatca gccttaattt ccatgccttc
    66901 cagttgaccg gatttcaggc atttagcatc gtggctccat tgcagatttt tcgccacgtc
    66961 ttcttttaaa ccaaactgcg ctaccagttc cggtgtcaga caagcatagc ttttgctcgc
    67021 gtcatcttca ccagcatacc agtaaatatc gtactcttcc gccaatggct gtttatttag
    67081 ttgaacctgt aaattgtatt ttccgggctc aacatatccc tggctggaaa aacgcttcag
    67141 atcaattttt gtgtcgcctt ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc
    67201 agcccagaca ctgctataac tccctgacat tgccagcgca atgcaccacg gaagaatccg
    67261 taatcgaaaa agagaatggt caggcataca cgtttctatc cgtttttatt cttgttcggt
    67321 caaaatccat ttaaatcccc catagcagtg tcgctatggg ggtatgcctt acaggtaagt
    67381 aatctggaag gtggtatttg cttcaaaatt acccagatct ggcgcatcag ctgcgccgac
    67441 cagccaggct ttaaagttca gtgtctgctt cgctttgcct ttgttggtag aagtgtcttt
    67501 cactattttc tggtcaatac ccatgccgct tttgtaagag gtacccagag agtcaccgat
    67561 cgccaggcta acattgttaa atgccgcacc ggtatcggta ttgaaaatgg tgtaataatt
    67621 gccgctattt gcagaagaaa cggtaccagt aaaggtagtg gtcattgttt cctgagtatc
    67681 aaatacgcaa tcttgcagac gaatctggaa acttttcggc gtggtggtgc cgttattttt
    67741 cagtttttcg gcaccgatat cacccatatc aacttccagt ttgtgatcgt ccggaacgat
    67801 agaacacact gagtcttcaa tggtgccgta aaaacgtgca gtaccgttgt tacccgcagc
    67861 gattgttgcc gtagaggtga acacgcccat caccattgcc gcagcaatag ctgttttaac
    67921 aaacttactc attatttgtc cttaaactat aaataattaa aaatataaac attgcaatac
    67981 attgattcag tcaatagcca atgtttatac ccagccattg aatacggcat gcgacccaaa
    68041 aagaacaggt cgtagaaaat tgtattcatt ggtgtggggc cgcatagtga tgtttccaca
    68101 cccctgatcc aatttaattt tactaaagag taaatgtaga tttaattaat atattgacag
    68161 aagaaaggtt atcttaagga catccttaaa aaatacggaa taagtattta gaaaaaatag
    68221 aaagtatatc aataatgaat atcaacagct taaaaaacaa acaatgaaca tataatgcga
    68281 ttggcattta tccacttttg acaatataat atataaacac tatagccaga atttaatcaa
    68341 gtttcaatta catcctgcct gattattcaa ctctattttc tctattatct gtattaataa
    68401 atgacactga gtgtcaaaca gacattattc atttcaacat gtaactttgg gcaaaatcaa
    68461 gatacgcgca gaagaataat ttccgaatga atgtagaaag tgttttattt atagataagg
    68521 agagtcttcg atagtagtga ggttgacaga tatccgctgc ctaatgagca attatgccca
    68581 atgaatctac ctcattgggc ataatttgga acacatcaga tagcttttgc catcttcaga
    68641 ttacacgggc tcatctgcca gttgaatgta ccgtttccgc tttcatcaag cgttacgctg
    68701 gcaatggccg aggtggtaaa cataggcggc gtttcgcccg gacataactc ggcaaccaga
    68761 tacccgacta acggtaagtg agagatcacc agtaccgaag caacaccttc attggtcaac
    68821 gcctgcaaat aggcactgac cagaccaaca tcgccgcagg gcgttaactc cggcagaact
    68881 tctgcactgg aaggcaggtt cagacaatcc cctacttctt ccagtgtttg ctcggctcgc
    68941 aggaacgggc tcaccagaac gcgttcgatt tccacttttt gacctttcag ccagttcgcc
    69001 atcaggcgag attcgtcaca accattagtg gtcagaggac gaaccgaatc actggcggca
    69061 tcgagggctg cgtcgccgtg acgcatgata aaaacttgca tattgcaccg cttttgttaa
    69121 ccagattcgc ccgttttctt tcatctgaaa ccagagaaag aaaacggtgg ccggcattgt
    69181 gccttatcca ttcaccgaat gaaacgctgt tttttacctc aatggcgtaa gtatagtcaa
    69241 tatgcgttta cattttgacc aacactccgc cattcagcgc ggattcatat agctttgacc
    69301 ttcttattgc aggtcagttg cagttgtttt ccaaaaactt tccccacgcg cgcccatctc
    69361 gaccaaacgc tcgcaagggg taaaacggga accatactgc gtggccagtc gttgcattat
    69421 tgcaaccact tcgcccgcgc cgagagaatc gatatagcgg aacggtccac cgagaaatgg
    69481 cggaaaacca atgccaaata ccgcaccaat atccccgtca cgcacgctac ggataacctg
    69541 ctcatcaaca caacgtactg cttcattcag catcaacatc acacaccgtt cagcaacctg
    69601 cggtgcggag agtcgcccct gcccttgtgc gccaatgagc gggtaaatgg caggatcgac
    69661 ctgttttttg cttttacgcc ctttctgacc ataaagatag aaaccccgac cattttttct
    69721 gcctttgcga tcgtcgttca aaattgaaga aacaacattt gcaggcgcgc taaaacgttc
    69781 tccataagcg gcttccagta caggaataat tttagtcccg gtgtcgattc ctacctcatc
    69841 caaaagttgg attgggccta ccggaaaacc aaatttcact agcgcggcat caatgtgctc
    69901 tacccgttca ccttcggtca gcatgcggat agcttcatta atgtaaggcg ctaagatgcg
    69961 attgacgtaa aacccggctt tgtcacgcac gacaatgggc gttttgccct gttttttcgc
    70021 cagttttacc gtagtggcga tggtttgcgc cgatgtaccc gcatgaggaa taatctctac
    70081 cagcggcatt ttttccaccg gactgaagaa atgcaggcca ataacttgct caggtcgcgc
    70141 ggcgtgagcg gcgatatcac caatcggtaa agatgacgtg ttcgaagcaa aaatggtatg
    70201 agcggcgcaa ttttgctcca cttccgccac catctgttgt tttaattcga gattttcaaa
    70261 caccgcttca ataatcagat cgcgatgggc aaagccgcga tagtccgtcg ttccggagat
    70321 taatgccagc tgtttgtcac gttcgctggc tttgagatga cgacggcgaa ctttgccctc
    70381 cagctgatcc caactgtact tcagcgcatg atttatgccc tgcgggttga tatctttaat
    70441 tctgaccgga agccccgctt tacaagcagt gacataagca ataccgccgc ccatcaagcc
    70501 accacctaaa attcccacgc tgtttaatgg cgcaggcggt gcatcactac cgggatcttt
    70561 cttcacgtcc gtactggcaa aaaagatact acgcaaagct tgcgattgcg gcgtcatcgc
    70621 cagttcgcca aaggcgcgag cttcagcgtc atagccgctg ctggtgccct gcgctaatcc
    70681 cgtttcaaca acctccagga tgcgttctgt cgccggataa ttgccttgag ttttgtgttc
    70741 tgttttcttg ccgaccattt tgaacagcag cgcacgacct aacggccccg ccagaatacg
    70801 ctcgcgtaca ggtagagggc gggaagatgg gcgatccttc ttcgccagct caacagcggc
    70861 ttccagcaaa atggagtgcg gaacaacgtc atccaccagc cccagcttta atgcctgttt
    70921 cgcccgaagt tgttttccgg tgagaatcat ctctaatgct gtactgacgc ctatcagacg
    70981 cggtaaacgc tgggtgccgc ctgaaccggg taacaatcca agttgtactt caggcaaacc
    71041 gagcaccgtt ttaggatcgt cagtacaaac gcgaccgtgg cacgccagcg ccagctccag
    71101 cccaccgccc aggcaagcgc catgaatagc cgcgataacc ggaatgggca aagcatgaat
    71161 ctccgccatc aactgttggc cctgccgcgc cagtgcttcc gcttcttgcg ccgttttgca
    71221 gttgccaatc atgttgatgt ctgctccggc gataaaattg tccggtttcg cggagataaa
    71281 caccacgccg cgcaactctt tgttttcacg gagttgctta ataatggcgc gcacctgcga
    71341 ggcaaattcc gccttcaggg tattcatttt ctcatccggt acgtcgatgg tgatgatagc
    71401 aatgttgtcc agacgaacat taagagtaaa cgctgatgcc atttccatta ttccgcctcc
    71461 agaaccattg ccgcgccaag cccaccggca gcacaggcgg taactaaacc aaatccaccg
    71521 ccgcgacggc gaagttcatg caatgtctgg gtaatcatcc gcgcgccggt cgccgcgaag
    71581 ggatgtccgt aagcaatcga accgccaagc acgttaaatt tgctatcgtc cacttcgcca
    71641 gtggcatgtg cacgccccag tacttcacga gcaaaacgtt cactacccag caactgaata
    71701 ttcgccagtg tctgagctgc aaaggcttcg tgcatatcga tcaatgtcag ttcgctcatc
    71761 gtcaaaccgg cacgttccag cgccagcggt gttgaccagg ctggaccgag caacatgtcc
    71821 tgccagacat caatcgcagt aaatgcgtag ctgcgcagat accccagcgg caccagccct
    71881 aattctttcg cccgggattc agtcatcagg atcaccgctg ccgcgccatc ggtcagcggc
    71941 gtactgtttg ccgccgttac cgttccgtgt ttgcgatcaa acgccgggcg cagctttgcg
    72001 taatcggcaa gcgaggaatt accgcgaata ttgttgtctt cgacaagcgg ttgtttataa
    72061 ggagggataa aggcagtcat cacctcttct ttgagttttc cgtctgacca tgcctgagcg
    72121 gcacgctgat gcgaacggtg cgctaatgca tcttgctgtt ctcgggtgat gccgtaggtt
    72181 ttcgccattt gttcggcggt gtcgcccatc cgcaagccag tagaatattc tgctaccgca
    72241 ggcggtacgg gcattaagtc gcgcaaacgc aggcgagaga agagtttcag gcgctggctc
    72301 atggttcgag ctttgttgac atcaaccagc acgtgcgcca gttttttact gacgccaatt
    72361 ggcaatacgg aagaggaatc tgccccaccg gcaatccccg ctcggatagt tcccgccatc
    72421 aggctttctg cgacgtttgc aaccgcctgg aaactggtag cgcaagcgcg gctgacgctg
    72481 taagcatcgg tatgcacatt cattccggta ccgagaacaa tttcacgcgc aatgttgggg
    72541 gcttcaggca tttgtacgac ctgaccaaag accagctgtt caatcacttc agcggggatc
    72601 tcgctgcgtg ccagcagttc gcctaccacc atcttcccta aatcaaccgc gggaatgcca
    72661 tgaaaagccg tcgcctggcg ggcaaaaggc gtacgtaaac cgctaacaat ggcgatacga
    72721 tcgccctggc gggtaaccag tggtaaaacc tgacccataa cactcccctg taaaaaaata
    72781 aataaagtgg tctgacctga tcatagtatt aaccattttt ttacatttag ccaagcggag
    72841 aaaaaggaaa gtgggagcta tgacacagtg aagacagaag aaagaaaaga aaacgcccct
    72901 gccagcaact gacaggggcg tacgtacccg agaggaatta acgcagaccc agctggaaga
    72961 tcagcatttc agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact
    73021 cttcagtgaa agtcggggag attttgcacg gttcggattc cacgctacgg gatttttcag
    73081 tcagcgccgc cagcgtttgt tctgcttctg cgcggtttgc aaacacgcgg ctgtaagacg
    73141 cggtgcagtc ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt
    73201 catcagcact acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat
    73261 aaacgttgtg gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa
    73321 tcggataagt gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatccg
    73381 cgagtgcgca acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa
    73441 aagatggaaa catacttgca acattctagc tggtccgacc tatactctcg ccactggtct
    73501 gatttctaag atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag
    73561 tttgtataaa aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag
    73621 tctgctctcg cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag
    73681 ttaaacgaat tttcttcctc tggcctgggc cgggcttatt caggggaagg cgcaattgcc
    73741 gatgatgcag gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca
    73801 ttttctgcgg gtgcggttta tattgacccg gatgtgaata tcagcggaac gtctccgtct
    73861 ggtcgtagcc tgcaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt
    73921 gttgcaccga ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggtctg
    73981 gcaacagagt ttaatgatac ttatgcaggc ggctctgtcg ggggtacaac tgaccttgaa
    74041 accatgaacc tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt
    74101 ggtttcaacg ccatctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag
    74161 ttggttgctg gtcagattat gcaatctcct gccgggcaaa ctcctcaagg gcaagcattg
    74221 gcagctaccg ccaacggtat cgacagtaat accaaaatcg ctcatctgaa cggtaaccag
    74281 tggggctttg gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca
    74341 ctgacctacc gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat
    74401 cgtgcgttta ataactacgg tttgccaatt cctaccgcga caggtggcgc aacgcaatcg
    74461 ggttatctga cgctgaacct gcctgaaatg tgggaagtgt caggttataa ccgtgttgat
    74521 ccacagtggg cgattcacta tagcctggct tacaccagct ggagtcagtt ccagcagctg
    74581 aaagcgacct caaccagtgg cgacacgctg ttccagaaac atgaaggctt taaagatgct
    74641 taccgcatcg cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt
    74701 atcgcctttg atgacagccc agttccggct cagaatcgtt ctatctccat tccggaccag
    74761 gaccgtttct ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac
    74821 gttggtgttt cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc
    74881 gagtctgaag gtaaagcctg gctgttcggt actaacttta actacgcgtt ctgataacgc
    74941 gttcgcctgg ataaagtcac ctgctatgca ggtgacttta actcccccac ttcaccgaaa
    75001 gtagtttcct cattgcttgc ctcctgagtt ttgcaaacag cctgatggca gcttgccctt
    75061 tcaaatcagt aagtggtatc cacagacaaa cctgaaggga aatgcatatt ttcaagcgtt
    75121 ctgctcgtcc ttctcaaaga gtttactttt ctgcatttcc aggatactca acccccctgg
    75181 ctattgtgcg cttatacact caaattaaag ataggttcta aataaatgag cgttttttga
    75241 tagtctattt cattaggtaa tatatatttg taacaaatca atcaacatag aataaaatca
    75301 tgctaccatc tatttcaatc aacaatacca gcgcagctta cccagaatcc atcaatgaaa
    75361 ataacaatga tgaaattaat ggattagtac aagagttcaa aaaccttttt aatggtaagg
    75421 aaggaataag cacctgtatt aaacatctac ttgagcttat aaaaaacgcc atacgagtaa
    75481 acgacgatcc ttatagattt aatattaata attcctcagt tacttatatt gatattggct
    75541 ccaatgatac agaccatatt actattggta tcgacaacca atagaattac ctgcgaacta
    75601 taaagacaaa gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga
    75661 tatcaataac aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg
    75721 ttttatcttc gataagatat cacacaaact cagacatacg gtaacggaat ttgatgagag
    75781 cgggaaaagc gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcgactc
    75841 tctcgctatt gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga
    75901 tcaggacgac taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata
    75961 ttgtagtgaa aacgccagga gtgcttcagc gtggtttgaa agcagtaaag ctatcatggc
    76021 agaatcattt gcaactggtt ccgatcatca ggttgtaaac gagctcaacg gggaaagact
    76081 gagagaacct aacgaagttt ttaaacgttt aggtcgagca ataagatata attttcaagt
    76141 ggacgatgca aaatttagac gcgataatgt aaaagaaata atttctactt tattcgctaa
    76201 caaagttgac gttgaccatc ctgaaaataa atacaaagac tttaaggatc tcgaagataa
    76261 agttgaaaaa agacttcaaa attgccaggc gaaatatcaa aatgaaatta atcaactatc
    76321 tgcactaggt gttaattttg atgatattta aaattatttt tccagttttt tgatttttca
    76381 acgcctgaat atcggaaata gtcagtgaca aa
//
LOCUS       DADWTH010000005        69006 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.005, whole genome shotgun sequence.
ACCESSION   DADWTH010000005 DADWTH010000000
VERSION     DADWTH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.005"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..188
                     /locus_tag="JNY02_000326"
     CDS             <1..188
                     /locus_tag="JNY02_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9431999.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNY02_000327"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNY02_000327"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBB9432000.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /gene="lpoA"
                     /locus_tag="JNY02_000328"
     CDS             1156..3192
                     /gene="lpoA"
                     /locus_tag="JNY02_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBB9432001.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="JNY02_000329"
     CDS             3150..3545
                     /locus_tag="JNY02_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBB9432002.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="JNY02_000330"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="JNY02_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBB9432003.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="JNY02_000331"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="JNY02_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBB9432004.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="JNY02_000332"
     CDS             complement(4854..5894)
                     /locus_tag="JNY02_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBB9432005.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="JNY02_000333"
     CDS             complement(5967..6602)
                     /locus_tag="JNY02_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBB9432006.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="JNY02_000334"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="JNY02_000334"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBB9432007.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTSAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="JNY02_000335"
     CDS             complement(7228..7671)
                     /locus_tag="JNY02_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBB9432008.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNY02_000336"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNY02_000336"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBB9432009.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGVAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="JNY02_000337"
     CDS             complement(8011..8514)
                     /locus_tag="JNY02_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB9432010.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9032)
                     /locus_tag="JNY02_000338"
     CDS             complement(8508..9032)
                     /locus_tag="JNY02_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBB9432011.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9241..10236
                     /locus_tag="JNY02_000339"
     CDS             9241..10236
                     /locus_tag="JNY02_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBB9432012.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10245..11123
                     /locus_tag="JNY02_000340"
     CDS             10245..11123
                     /locus_tag="JNY02_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBB9432013.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11204..12211
                     /locus_tag="JNY02_000341"
     CDS             11204..12211
                     /locus_tag="JNY02_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBB9432014.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSIQQAFVKLRRGEIRQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNY02_000342"
     CDS             complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNY02_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBB9432015.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13727..15616)
                     /gene="deaD"
                     /locus_tag="JNY02_000343"
     CDS             complement(13727..15616)
                     /gene="deaD"
                     /locus_tag="JNY02_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBB9432016.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNY02_000344"
     CDS             complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNY02_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBB9432017.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNY02_000345"
     CDS             complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNY02_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBB9432018.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNY02_000346"
     CDS             complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNY02_000346"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBB9432019.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNY02_000347"
     CDS             complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNY02_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBB9432020.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNY02_000348"
     CDS             complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNY02_000348"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBB9432021.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNY02_000349"
     CDS             complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNY02_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBB9432022.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNY02_000350"
     CDS             complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNY02_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBB9432023.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNY02_000351"
     CDS             complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNY02_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBB9432024.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNY02_000352"
     CDS             complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNY02_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBB9432025.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25945..26021)
                     /locus_tag="JNY02_000353"
     tRNA            complement(25945..26021)
                     /locus_tag="JNY02_000353"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25985..25987),aa:Met,seq:cat)
     gene            26369..27712
                     /gene="argG"
                     /locus_tag="JNY02_000354"
     CDS             26369..27712
                     /gene="argG"
                     /locus_tag="JNY02_000354"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBB9432026.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27720..29327)
                     /locus_tag="JNY02_000355"
                     /pseudo
     CDS             complement(27720..29327)
                     /locus_tag="JNY02_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29805..29891)
                     /locus_tag="JNY02_000356"
     tRNA            complement(29805..29891)
                     /locus_tag="JNY02_000356"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29855..29857),aa:Leu,seq:gag)
     gene            complement(29906..30238)
                     /gene="secG"
                     /locus_tag="JNY02_000357"
     CDS             complement(29906..30238)
                     /gene="secG"
                     /locus_tag="JNY02_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBB9432027.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSTPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="JNY02_000358"
     CDS             complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="JNY02_000358"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBB9432028.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31796..32644)
                     /gene="folP"
                     /locus_tag="JNY02_000359"
     CDS             complement(31796..32644)
                     /gene="folP"
                     /locus_tag="JNY02_000359"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBB9432029.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="JNY02_000360"
     CDS             complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="JNY02_000360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBB9432030.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="JNY02_000361"
     CDS             complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="JNY02_000361"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBB9432031.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35523..35816
                     /gene="yhbY"
                     /locus_tag="JNY02_000362"
     CDS             35523..35816
                     /gene="yhbY"
                     /locus_tag="JNY02_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBB9432032.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(35972..36448)
                     /gene="greA"
                     /locus_tag="JNY02_000363"
     CDS             complement(35972..36448)
                     /gene="greA"
                     /locus_tag="JNY02_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBB9432033.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36696..38129
                     /gene="dacB"
                     /locus_tag="JNY02_000364"
     CDS             36696..38129
                     /gene="dacB"
                     /locus_tag="JNY02_000364"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBB9432034.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="JNY02_000365"
     CDS             complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="JNY02_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBB9432035.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39503..40468)
                     /locus_tag="JNY02_000366"
     CDS             complement(39503..40468)
                     /locus_tag="JNY02_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBB9432036.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLSPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="JNY02_000367"
     CDS             complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="JNY02_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBB9432037.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="JNY02_000368"
     CDS             complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="JNY02_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBB9432038.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41443..42414
                     /gene="ispB"
                     /locus_tag="JNY02_000369"
     CDS             41443..42414
                     /gene="ispB"
                     /locus_tag="JNY02_000369"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBB9432039.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42642..42920
                     /gene="sfsB"
                     /locus_tag="JNY02_000370"
     CDS             42642..42920
                     /gene="sfsB"
                     /locus_tag="JNY02_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBB9432040.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPRTHEFIDRTQLMRSYTKPKK"
     gene            complement(42968..44227)
                     /gene="murA"
                     /locus_tag="JNY02_000371"
     CDS             complement(42968..44227)
                     /gene="murA"
                     /locus_tag="JNY02_000371"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBB9432041.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="JNY02_000372"
     CDS             complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="JNY02_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBB9432042.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="JNY02_000373"
     CDS             complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="JNY02_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBB9432043.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="JNY02_000374"
     CDS             complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="JNY02_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBB9432044.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="JNY02_000375"
     CDS             complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="JNY02_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBB9432045.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="JNY02_000376"
     CDS             complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="JNY02_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBB9432046.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHVPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="JNY02_000377"
     CDS             complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="JNY02_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBB9432047.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48008..48973
                     /locus_tag="JNY02_000378"
     CDS             48008..48973
                     /locus_tag="JNY02_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBB9432048.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLLLGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48987..49973
                     /gene="kdsD"
                     /locus_tag="JNY02_000379"
     CDS             48987..49973
                     /gene="kdsD"
                     /locus_tag="JNY02_000379"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBB9432049.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49994..50560
                     /gene="kdsC"
                     /locus_tag="JNY02_000380"
     CDS             49994..50560
                     /gene="kdsC"
                     /locus_tag="JNY02_000380"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBB9432050.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50557..51132
                     /gene="lptC"
                     /locus_tag="JNY02_000381"
     CDS             50557..51132
                     /gene="lptC"
                     /locus_tag="JNY02_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBB9432051.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51101..51658
                     /gene="lptA"
                     /locus_tag="JNY02_000382"
     CDS             51101..51658
                     /gene="lptA"
                     /locus_tag="JNY02_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBB9432052.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51665..52390
                     /gene="lptB"
                     /locus_tag="JNY02_000383"
     CDS             51665..52390
                     /gene="lptB"
                     /locus_tag="JNY02_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBB9432053.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52438..53871
                     /gene="rpoN"
                     /locus_tag="JNY02_000384"
     CDS             52438..53871
                     /gene="rpoN"
                     /locus_tag="JNY02_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBB9432054.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53894..54181
                     /gene="hpf"
                     /locus_tag="JNY02_000385"
     CDS             53894..54181
                     /gene="hpf"
                     /locus_tag="JNY02_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBB9432055.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54299..54790
                     /gene="ptsN"
                     /locus_tag="JNY02_000386"
     CDS             54299..54790
                     /gene="ptsN"
                     /locus_tag="JNY02_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBB9432056.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54836..55690
                     /gene="rapZ"
                     /locus_tag="JNY02_000387"
     CDS             54836..55690
                     /gene="rapZ"
                     /locus_tag="JNY02_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBB9432057.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55687..55959
                     /gene="npr"
                     /locus_tag="JNY02_000388"
     CDS             55687..55959
                     /gene="npr"
                     /locus_tag="JNY02_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBB9432058.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56172..56804
                     /gene="yrbL"
                     /locus_tag="JNY02_000389"
     CDS             56172..56804
                     /gene="yrbL"
                     /locus_tag="JNY02_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBB9432059.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="JNY02_000390"
     CDS             complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="JNY02_000390"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBB9432060.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="JNY02_000391"
     CDS             complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="JNY02_000391"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBB9432061.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="JNY02_000392"
     CDS             complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="JNY02_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBB9432062.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSHRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60841..61770)
                     /locus_tag="JNY02_000393"
     CDS             complement(60841..61770)
                     /locus_tag="JNY02_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBB9432063.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62445..66905
                     /gene="gltB"
                     /locus_tag="JNY02_000394"
     CDS             62445..66905
                     /gene="gltB"
                     /locus_tag="JNY02_000394"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBB9432064.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66918..68336
                     /gene="gltD"
                     /locus_tag="JNY02_000395"
     CDS             66918..68336
                     /gene="gltD"
                     /locus_tag="JNY02_000395"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBB9432065.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68520..68999
                     /locus_tag="JNY02_000396"
     CDS             68520..68999
                     /locus_tag="JNY02_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBB9432066.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16846 a        18750 c        17156 g        16254 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aattagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aacctgtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgtcagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca ggggttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac
     8881 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
     8941 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
     9001 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
     9061 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
     9121 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
     9181 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
     9241 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
     9301 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
     9361 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
     9421 cttcacatct cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
     9481 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
     9541 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
     9601 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
     9661 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
     9721 gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg
     9781 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
     9841 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
     9901 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
     9961 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    10021 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    10081 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    10141 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    10201 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    10261 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    10321 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    10381 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    10441 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    10501 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    10561 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    10621 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    10681 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    10741 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    10801 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    10861 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    10921 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    10981 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    11041 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    11101 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    11161 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    11221 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    11281 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    11341 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    11401 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    11461 ctcaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    11521 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    11581 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    11641 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    11701 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    11761 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    11821 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    11881 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatac agcaagcctt
    11941 tgtgaagctg cgccgtggcg aaatcaggca actgccgccg ccgattcaaa atatggatca
    12001 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    12061 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    12121 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    12181 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    12241 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    12301 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    12361 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    12421 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    12481 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    12541 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    12601 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    12661 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    12721 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat
    12781 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    12841 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    12901 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    12961 acgacgccaa gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    13021 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    13081 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    13141 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    13201 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    13261 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    13321 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    13381 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    13441 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    13501 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    13561 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    13621 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    13681 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    13741 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    13801 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    13861 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat
    13921 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    13981 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    14041 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    14101 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    14161 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    14221 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    14281 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    14341 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    14401 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    14461 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    14521 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    14581 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    14641 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    14701 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    14761 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    14821 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    14881 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    14941 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    15001 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    15061 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    15121 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    15181 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    15241 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    15301 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    15361 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    15421 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    15481 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    15541 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    15601 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    15661 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    15721 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    15781 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    15841 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    15901 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    15961 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc
    16021 cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt
    16081 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg
    16141 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    16201 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    16261 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    16321 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    16381 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    16441 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    16501 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    16561 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    16621 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    16681 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    16741 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    16801 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    16861 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    16921 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    16981 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    17041 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    17101 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    17161 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    17221 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga
    17281 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    17341 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    17401 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    17461 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    17521 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    17581 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    17641 cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    17701 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    17761 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    17821 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    17881 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    17941 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    18001 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    18061 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    18121 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    18181 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    18241 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    18301 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    18361 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    18421 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    18481 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    18541 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    18601 gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt
    18661 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    18721 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    18781 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    18841 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    18901 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    18961 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    19021 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    19081 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    19141 tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    19201 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    19261 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    19321 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    19381 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    19441 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    19501 gtacgttacg caaagtgtta aataatttac gcgacgttaa gcggcagtat tctactcgta
    19561 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    19621 gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt
    19681 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    19741 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    19801 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    19861 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    19921 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    19981 caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc
    20041 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    20101 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    20161 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    20221 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    20281 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    20341 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    20401 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    20461 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    20521 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    20581 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    20641 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    20701 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    20761 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    20821 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    20881 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    20941 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    21001 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    21061 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    21121 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    21181 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    21241 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    21301 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    21361 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    21421 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    21481 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    21541 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    21601 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    21661 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    21721 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    21781 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    21841 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    21901 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    21961 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    22021 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    22081 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    22141 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    22201 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    22261 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    22321 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    22381 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt
    22441 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa
    22501 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    22561 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    22621 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    22681 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    22741 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    22801 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    22861 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    22921 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    22981 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    23041 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    23101 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    23161 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    23221 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    23281 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    23341 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    23401 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    23461 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    23521 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    23581 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    23641 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    23701 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    23761 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    23821 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    23881 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    23941 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    24001 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    24061 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    24121 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    24181 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    24241 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    24301 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    24361 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    24421 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    24481 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    24541 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    24601 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    24661 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    24721 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    24781 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    24841 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    24901 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    24961 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    25021 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    25081 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    25141 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    25201 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    25261 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    25321 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    25381 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    25441 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    25501 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    25561 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    25621 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    25681 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    25741 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    25801 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    25861 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    25921 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    25981 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    26041 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    26101 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac
    26161 gtgaatgaat atccagttca ctttcatttg ttaaatactt ttgccttccc ctgctctccc
    26221 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    26281 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    26341 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    26401 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    26461 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctaggccagc cagacgaaga
    26521 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    26581 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    26641 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    26701 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    26761 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    26821 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    26881 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    26941 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    27001 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    27061 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggttg cgctgaacgg
    27121 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    27181 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    27241 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    27301 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    27361 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    27421 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    27481 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    27541 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    27601 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc
    27661 tgccacttca ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    27721 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    27781 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    27841 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    27901 ataaggtaat tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca
    27961 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    28021 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    28081 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    28141 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    28201 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    28261 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    28321 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    28381 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    28441 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    28501 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    28561 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    28621 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    28681 atacttgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    28741 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    28801 gcaaatcacg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    28861 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    28921 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    28981 aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttcac aacttcatcc
    29041 ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    29101 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    29161 agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag gacgaagaac
    29221 agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat accaaaaaca
    29281 ataacacaaa gatacaacaa ccaatgagat ttaaaatctt ctagcaaatt tgttgaatac
    29341 tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat gttcaatttt
    29401 atatcagaca tgtttttcaa ctctgaacca tatcagaccg aaacactgtt agttttttct
    29461 ttttaaatga tgcgaccatt gcgttctcag taattaaata acattatcaa tgcgtctcta
    29521 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
    29581 gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat
    29641 gtcagcagta gggaaaagtt tataactaag gcgtaccggc accatcgttt caaggtacca
    29701 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcat cagaccagga
    29761 ccaaaacgaa aagagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg
    29821 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
    29881 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
    29941 cagctggctg agtttgttcg gttttcgccg gtgtactcag attttcccat tcgctacctc
    30001 tattggtttt gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
    30061 ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
    30121 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
    30181 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
    30241 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
    30301 ttaatctttc ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg
    30361 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgccgag
    30421 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
    30481 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
    30541 cacgcgaatt aacggttcgg tgccggattt acgcagcaac acgcgtccac ggttgcccag
    30601 cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagcg gatcgccgct
    30661 acctgcggtg taacgtacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag
    30721 gtcgtgcagg ctcatatggt tacgtgccat cgccgccagc acctgcaagc cagcaacgat
    30781 gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggaattct ctgcaccgat
    30841 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
    30901 aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
    30961 cacagcgcca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
    31021 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
    31081 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
    31141 gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat
    31201 aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc
    31261 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
    31321 aaactcgata tagcgacccg cggcatcaac gatacggctg gctttaccca gttctgccga
    31381 atcaacgcag ctgatctcct tttccatttc cgcttcgatg gcctcttcta ccgcatccgg
    31441 cagtttagtg ccgtcgatag agaagaattt aatcccatta tcgtagaacg ggttatgcga
    31501 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
    31561 tgttggcatc gggccagtga agagtgcgga aaggcccgct gccgccagac ccgcttccag
    31621 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga
    31681 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
    31741 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
    31801 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
    31861 tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac
    31921 aggccagact accgctcagg cgctcggacg gccccacgtt cagcagctgc ccaatcatcg
    31981 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
    32041 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
    32101 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
    32161 tcacttctgc aaagacatcg tcatacttcg gagcttcctg catggttttt ggatttccct
    32221 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt
    32281 cggaaaggga gcggatatca ttaataatgt gagcgccaac tttcgctgac tcacggatga
    32341 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
    32401 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cctgggcgcg
    32461 tggactcgcc accaacgtca atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt
    32521 tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
    32581 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
    32641 tcatgatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
    32701 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
    32761 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
    32821 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
    32881 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
    32941 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
    33001 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
    33061 atgtttcgct ttcgctacgc tacggccgag gaatacttca ccttcttctt ccgcgtacag
    33121 cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg ccaggttggt
    33181 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt agatgatctc
    33241 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
    33301 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
    33361 aatcgtcact ttatgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
    33421 cgcttcgtgg taagccgttg attctttctg cgcttccgtc atcaccatgg agcgacgttc
    33481 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
    33541 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
    33601 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
    33661 acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc cgaccacaac
    33721 ctggcggtcg aaacggccag gacgcagcag ggccgggtcg agaacgtccg gacggttagt
    33781 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
    33841 gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc
    33901 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
    33961 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
    34021 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc
    34081 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
    34141 gcggctcggc tcgcgcagat actctaccag ttcagcaact tcttcttttg cttcgtcgca
    34201 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct
    34261 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
    34321 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
    34381 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
    34441 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
    34501 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
    34561 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
    34621 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
    34681 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
    34741 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
    34801 ttctcgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
    34861 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
    34921 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
    34981 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
    35041 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
    35101 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
    35161 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
    35221 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
    35281 aagtttaaac caggcacggg aacgtaatcc ctttttctgt gcctgttgaa catatttatc
    35341 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
    35401 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
    35461 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
    35521 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
    35581 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
    35641 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaacaaaaa
    35701 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
    35761 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
    35821 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
    35881 aaatgccacg ctctgttcgt tgataaaagg ccgcagagcg gcctttttcc tttctttaca
    35941 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
    36001 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
    36061 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
    36121 cgccaacgat gcgataagtc tgttcttcgt cagaatccag attcagcacc gttacggtag
    36181 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgtttg
    36241 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
    36301 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
    36361 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
    36421 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
    36481 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
    36541 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatggt
    36601 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
    36661 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
    36721 gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag tacgttactc
    36781 aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggtgcg tcggcccccg
    36841 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacccagaaa gtgattactg
    36901 cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca
    36961 aaggcaatgt ggaaaacggc atacttaagg gtgacttagt ggcgcgattt ggtgccgatc
    37021 cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
    37081 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
    37141 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgct gccgccatag
    37201 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaagcctggt gatatggctt
    37261 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
    37321 gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt gccaggcgac ctgaaccgct
    37381 ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttggcgtgc
    37441 aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag gcgggtatca
    37501 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
    37561 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
    37621 tgattgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
    37681 ggcgggctgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
    37741 gtaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
    37801 ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca
    37861 tgctgccgct tgcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
    37921 gcgtggatgg caaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
    37981 gattcattac cacagcgagc gggcaacgaa tggcatttgt acaatatctt tctggctatg
    38041 cagtaaaacc cgcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
    38101 tgtacaaaga tatttatcag aacaattagt caaaaagaaa ccccggcgca tggccggggt
    38161 ttcagattat tgacaaagtg cgctttgttt atgccggatg cggcgtgaac gccttatccg
    38221 gcctacaaaa tcgtgcaaat tcaatatatt gcaatcatct tgtaggcctg ataagtgtag
    38281 cgcatcaggc ttatttggca ttggtcatta gtgattaacg cttgtaaatg aactcaacgc
    38341 cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcttc atcatcctct tcagcaatct
    38401 cttcaagctg ctgacggtga taatcatccc acatgaattc gactttctct ggctgtttcg
    38461 cttcttcagc ctgcacgacc gggttttcaa tgataaaggt catcacatcc cagcagagat
    38521 ctttcacgcc cagtccgctc gccgcagaga tcagataata tttatcctcc cagcccagcg
    38581 cttcagcgat cgctttcgct ttctcttcgg cttctgcctt atccagcaga tcgatcttgt
    38641 tgaagactaa ccaacgcggt ttcgccgcca gatcctggct gtatttttcc agctcgctga
    38701 taataatacg cgcgttttca accggatcgg tgccgtcaat cggatcgata tcgatgaggt
    38761 gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg aatgcccaga cctgcgcctt
    38821 ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac gaagctcttt tcgttgtcca
    38881 ttcgtaccac acccagactt ggcaccagag tggtaaacgg ataatccgcc actttcggtt
    38941 tagccgccga taccgcacga ataaaggtcg atttacccgc gtttggcatc cccaacatac
    39001 cgacgtcagc cagcagcatc agctccagca gcagctcacg cttatcgccc ggcgtaccgt
    39061 tagttttctg ccgtggtgta cggttaactg acgatttgaa acgggtattg cccagaccgt
    39121 gccagccgcc cttagcaacc agcagacgct gaccgtgttt agtcatatca cccatggttt
    39181 caccagtacc ctggtcgatt acacgcgtac ctaccggcac tttaatcgtc acgtctttac
    39241 cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc gcgctctgca cggaaagatt
    39301 tttcaaaacg ataatcgata agcgtgttca ggttctcgtc ggcttccatc catacgtcac
    39361 caccatcacc gccgtcgccg ccatccgggc cgcctttcgg aatatacttt tcgcggcgga
    39421 agctcacgca accattaccg ccatcacctg caacgaccag aatcgatgct tcatcaacaa
    39481 acttcatttt attctccgta aatcattcgc ctgcgcgggg ttgcgatacc accgttgtat
    39541 gcttacgtaa tccgccccaa atacgatgac caatggcgga atacatcgcg cccgcaacca
    39601 cgacaaacgc accgagataa cctaaaaggt ttaacatcgg tctggcgaag aaatcaggcc
    39661 aggccagtga taaaagatct gaaaaaaaca gcgtaaacag tggggtgagc gtgatgatcg
    39721 cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc caaggcgcca tatcctacca
    39781 aggtattcag tccgcaaaaa attaaacatg cgagctgcca gtggctaagc tgcgctatca
    39841 ctccaggctt tgccagaggg aagagcgcga ttgtacataa agtgtacagt aaaaacagga
    39901 tctgcggtga ggccagccga cgcaataaaa ccttttgcgc cacgccataa ctcacccaaa
    39961 ccgtcgccgc accgacccca aagataactc cccaggtgta atcggtgagc tttgtaaata
    40021 tctcgaccag actggtgtta aaaaacatca ccagaccgct caggagcatc aatgccccta
    40081 caacctgagt gctgcgcatt ttctctttca ggataaatac gctggcaacc atcatgccaa
    40141 ctggcgagag ttgcccaatc acctgcgaag cggtcggact caggtattgc aaggatgagc
    40201 tgaacaggat gaagttccca aacagcccgg cggtcgccac tgccaacaaa atcaaccagc
    40261 gtggcttacg aaacacgcgt aatggcgaca acctcttctt caccgcaaga atggcaccaa
    40321 ggccaatact cgccatcaag aaacggtaaa acacgattgt cggaggttcc atcacctcca
    40381 gcacctgctt cattgcgatt ggcaacgccc cccagcaaat tgctgtggta agcgccaaaa
    40441 gaatgccaat gcctgcctgc tgcttcatac cgtattccct gcaaagcgca tttgcggatt
    40501 aacgtcgcac catgcgcatt tttaccggtt atcgaatgta aaaagccccg caacgtgttg
    40561 cggggctttc atccgttacc gggacgcgaa aaacttattc agcttcgatg ctgataaatt
    40621 tacggttttt cgggccttta acttcgaatt tcactttacc gtctgctttg gcaaacagag
    40681 tgtggtcacg accgcaacct acgttagcgc cagcgtggaa tttggtacca cgttgacgaa
    40741 cgatgatgct acccgccaga acggattcgc caccgaaacg cttaacgccc aggcgtttag
    40801 cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt tttatgtgcc atttgaaatc
    40861 tctcctcagg tcttaggcgc tgatgccagt aattttcaca tcagtgaacc actgacgatg
    40921 gccctgctgc ttacgatagt gtttacgacg acgaaactta acgattttaa ctttctcgcc
    40981 acgaccgtga gcaacaactt cagctttgat tacgccgcca tcaacgaaag gaacgccgat
    41041 tttgacttct tcaccgtttg cgatcatcag cacttcagcg aactcaacag tttcgccagt
    41101 tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg cttactcggt gttgtttacc
    41161 accactttgg aaaaccgcgt acatataaaa ctccgcttcc gcgcacacct tttcaatgat
    41221 tcagagtgcg ctataaatat tcacaatagg gcgcgaatat tacgcaaaac gcacgccttt
    41281 gacaagtgct acagtcaata cacgaagaaa aaaaacacaa cttgtacggt aacgtttatc
    41341 tgtgccattt tttcagtaca attaccctat attcctaacc ataaacccta agttgccttt
    41401 gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc
    41461 aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg caatccttga gcagcttaat
    41521 tccgacgtcc aactgatcaa tcagttaggc tattacatcg tcagcggcgg cggtaaacgt
    41581 attcgtccga tgattgctgt actggctgca cgagctgttg gctatgaggg aaatgcgcat
    41641 gtcaccattg ctgccctgat cgagtttatc cacacggcga ctctgctaca cgacgacgtt
    41701 gtggatgaat cagatatgcg caggggtaaa gctaccgcca acgccgcatt tggcaatgcc
    41761 gccagcgtgc tggtaggcga ttttatttat acccgcgcgt tccagatgat gaccagcctc
    41821 ggttcactca aagtgctgga agtcatgtca gaagctgtaa acgtcatcgc agaaggtgaa
    41881 gttctgcaac tgatgaacgt taacgatccg gacatcactg aagaaaacta catgcgcgtt
    41941 atctatagca aaaccgcgcg tctgtttgag gctgccgcgc agtgttccgg gattctggct
    42001 ggctgtacgc cggaggagga gaaaggcctg caggattatg ggcgctatct cggcactgct
    42061 ttccagttga tcgacgattt actcgattac aatgccgatg gcgaacagtt aggtaaaaac
    42121 gtcggcgacg atctgaacga aggtaaaccg acgctgccgc tgctgcatgc gatgcatcat
    42181 ggcacaccag aacaggcaca gatgatccgt accgccatcg aacaaggtaa cggtcgccat
    42241 cttctggaac cggttctgga agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag
    42301 cgcgccgagg aagaagcaga caaagccatc gcagcgttac aggtgctccc ggacacccct
    42361 tggcgagaag cactcatcgg cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct
    42421 cccttcatcc cgcgcagtgc gcgggatgat tccagtaaat tccataaaaa cactaattca
    42481 gcgctataac acctgaaaaa aagggcgatg aatctttata tattctgaat attcacactc
    42541 tttacaggaa ctttttagcg caataggcca tcaggagtat agtgatgccc gacagaagaa
    42601 gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa gatggaaagt aatttcattg
    42661 actggcatcc cgctgacatc attgcaggtt tgcgcaagaa aggaacgtca atggcggcgg
    42721 aatctcgcag aaatggtttg agttcctcaa cgctggcaaa cgcattatcg cgcccatggc
    42781 cgaaaggaga gatgattatt gcgaaagccc tgggaactga cccctgggtt atctggccat
    42841 cacgctacca tgatccgcgg acccatgagt ttatcgacag aacgcagttg atgcgtagct
    42901 acactaaacc gaaaaaatga gtggtctggc ggtagccccg cgaacggggc tgccagctct
    42961 cagacgatta ctcgcctttc acacgctcaa tatttgcacc taaagcgcgc agtttgtctt
    43021 caatgcgttc gtagccacga tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg
    43081 caatacagcc agccagcacc aggcttgctg atgcacgcag atcggttgcc ataacctgtg
    43141 caccagaaag tttttcaaca ccatgacaaa taacggtatt gctttcgatt tcggcgtgcg
    43201 cgcccatacg gctcagctct ggcacatgca taaagcggtt ttcaaagacc gtttcggtga
    43261 tgaacccggt cccttcagcc accaggttca acagcgtgaa ctgggcctgc atatcggtcg
    43321 ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt cggacgtttg ccatgcatat
    43381 ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc agcatcacgc agtttcgcca
    43441 gcacggcgtc cagagtatct ggctgcgcgt tacggcagat aattttgcca cgagagatcg
    43501 ccgccgccac caggaaagta ccggtttcga tacgatccgg cagcacgcga tagacaccgc
    43561 cgcctaaacg ttccacacct tcgatgacga tacgatcggt gccctgaccg ctaattttcg
    43621 cacccagcgt aatcaggaag ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt
    43681 caataatcgt ggtgccttcc gccagggttg cagcacacat gatggtcacc gttgcgccaa
    43741 cgctgacttt atccatcacg atatgtgcgc ctttcagacg accatcgacg gaagctttaa
    43801 cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc gaggccagaa atgtgtagat
    43861 caaccggacg agcaccgatc gtacaaccgc caggtagtga aacttgcccc tgaccaaagc
    43921 gcgctaccag cggccccagc gcccagatag aagcacgcat ggttttaacc agatcgtaag
    43981 gtgcgcagaa tacattaacg tcgcgggcat caatatgcac agaaccatta cgttctactt
    44041 tcgcacccag ctggcttagc agtttcattg atgtatcgac gtctttcagt ttcgggacgt
    44101 tctggatctc taccggttct tccgccagta gtgcggcaaa aagaataggc agagcagcat
    44161 ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt tggcccctga acacgaaatt
    44221 tatccattta gtttgttctc agttaacaat tcatatccgc taccggcgaa tcgcccatag
    44281 ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc aggggtatac gctttgatcg
    44341 acacagcatg aatgcggtta tccgcaatat attccatcag cgggccatag accgtctgct
    44401 gttttttaac ccgactcata ccgtcaaaca actcacccac ggcaataacc tgaaagtgac
    44461 tgccatcgcc ggaaacgtgg acttcctgga gggagagagc gttcatcaac acgctctgaa
    44521 tttcattatt ttccatggga tcttcaatca tcagttaata aaccagcgaa acatcttaga
    44581 gcaaagttgc gctggcataa ataagcaaaa agcctcgctg ataaatcaga caaggctcga
    44641 cttgcaggca ggtttgccgg acaggcggtt aacgccatat ccggcctgaa aaaatttaac
    44701 gaggcagaac atcagcaggc aaattataca atttcgcgag ggtatacact ttgtcgttta
    44761 ccccctgaag cgtcacgttg ttgccctgct ttttcgccag atcgataaga tggagcagca
    44821 gtgccagtcc ccccgtatcc acgcgggaca cacggctaag atcgatgcag gtaatcccct
    44881 tcaccgcttc ctcacgcatt tcccaaagcg gtagcagaac gtcctgatcc agctctccgg
    44941 ataacgccag cgtgtcaccc gtctgcatcc agctcagtga ctcgctcatt attttttctc
    45001 ttccagagtg attttctgtt gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc
    45061 tttggtacgc agcagcgttc cccactcgtt ttgtttggtg gtgatcatac tgacgccttc
    45121 agcaatcatg tcgtaagctt gccagtttcc cgtctgggag tttttacgcc actggaagtc
    45181 cagacgcacc ggcggacggc cattcgggtc aataatggta acgcgaatag gcacaatggt
    45241 tttatcgccc agcggctgtt ctggcgcaat ctgataggtt tgaccgtgat acatcgccag
    45301 cgcctgaccg taagcctgct tcaggtactc acggaaagcg gcaaagtagg cttcacgttg
    45361 agcaggggtc gcactcttgt aatactggcc cagcaccagc gcaccggcgt atttcacctg
    45421 tacgtatggc agcagttcct gatcaacaat ggtacgcaga taatccgggt tggcccgaat
    45481 ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt
    45541 ataaggattg gtctggtctg ccgcggttgc cgcactcaga ggtgcaatca ccagcaaagc
    45601 gaccatcatt aaacgtttaa acatgcgtcg gttctcctga aattatttcg ttgtacccac
    45661 aggttcagtg gtttcattat tacctggcgc agcagctggc gcatcgccac tattcttatt
    45721 gtcatcgcct ttactaccgt aaaggaactg accaatgaga tcttccagca ccatcgcaga
    45781 cttggtgtcc tgaattgtat cgccatcctt caggatagca gtccccagtt ccgggtcttc
    45841 aaaaccgacg tttaatgcca gatattgttc tcccagcagg ccggaagtac gaatgctcag
    45901 cgaactggta tcaggaatgt ggttataacg ttgttcaatt tccagcgtta cgcgcggcag
    45961 ataggttttc gggtccagcg taatatccgc cacccggccc acaacaacac caccaatgct
    46021 gaccggagag cgcgctttca ggccgccaat gttatcgaac gtcgcataaa gcgtgtaggt
    46081 cgattcagta cgtatggacg tcacgttcgc cgccttcagg caaacaaaca gcgccgccag
    46141 cagtgctgct aataaaaaga tacccaccca aatttcattt tttttcgttt gcatgaactc
    46201 aattcccaaa catcaatgcg gtcagcacaa aatccagccc cagaacagcc agagacgagt
    46261 ggacaacggt gcgagtggtt gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc
    46321 cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac cacgctctta atcagacagt
    46381 tgaccagatc catacgccag tcgacggcat tttgcattgc cgaccagaag aacccgctat
    46441 caatgccttt ccaactgacg ccgaccagcg atccgcccca gatccccacg gcgacgaaga
    46501 taaccgtcaa cagtggtaat gaaataaccc cagcccagaa acggggagaa ataacccgac
    46561 gcagcggatc caccgccatc atctccatac tggagagttg ctctgtagcg cgcatcaggc
    46621 cgatttctgc ggttaacgcc gaaccagcac gcccggcaaa caacagcgcg gcaaccaccg
    46681 gccccagttc acgcagtagc gataacgcca ccagcatacc cagactggtt tccgcgctat
    46741 aagtggtcag aaccagataa ccttgcagcc ccaacaccat tccgatgaac acgccagaaa
    46801 ctacaataat cagcatcgac aggacgccga cattatagag ctggcgcacc agcagcggca
    46861 catgtttgcg aaattccggt ttgccgacca gcgcattgaa taacattaac ccggcccgcc
    46921 cgaacgttct cagggtttta atccctttat gtccgagcga cgccagcgca tttaacagca
    46981 tgagtggctt aactccctgg taaaagatca gcgtgataat cgccggcagg atagcggaac
    47041 ggaacaggcc cgtcagctat cccgtccaga aactgacgta cgcgcggatc aggattcgtc
    47101 tgcaacgcct gggcactgcc atgagcgaca atttttttgt ccgccaggat ccaggcgtga
    47161 tccgcaatac ttaacacttc cggcacatcg tgagaaacca ccacacaagt cacgcccagc
    47221 gcgctgttca gctcagaaat cagcttcacc agtacgccca tggtgatggg atcttgccca
    47281 acaaaaggtt catcaaacat gatgagatcc ggctccagcg caatcgcacg cgccagcgct
    47341 gcacgccgcg ccatcccacc ggaaagttca gaaggcatta gtttagccgc tccacgcagc
    47401 cccacggcct ccagcttcat catcaccgta ctatgcaaca atggcgcggg aagttgggta
    47461 tgttcgcgca gtggataggc gacgttgtca aatacgttca tatcagtgaa caacgccccg
    47521 gactgaaata acatgctcat ccgtttacgc actgtataca ggcgcgaacg agacatcgcc
    47581 ggaatattct caccatcgaa aaggatctca ccatgatctg gtgcgatttg cccgccaatc
    47641 agacggagta gcgtcgtttt accgatgccc gatggcccca tgatcgccgt gatcttccct
    47701 cgcggcacgg tcagggaaat attatcgaag atgcagcgat tgccacgcgt aaaactgaca
    47761 tcgcgcatat cgactaaatt cgccacagac tgctccataa ttcacccttc gtcttgcgtt
    47821 gattttctaa gcatggcgct caatttaacc ttgaacccaa catatttaca gaatattacc
    47881 cgccgtggtt agcgaaagct ggcatttgtt ttacttttta gccgcataaa gtcaaaatta
    47941 agaatccgtt acggctttct gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg
    48001 acgttagatg cttttagcta cggcactgtt aattgttggt ttacttttgg tcgtttacag
    48061 tgccgaccgc ctggtttttg ccgcgtctat tctttgccga acctttggca tcccgccgct
    48121 gatcatcggc atgacggtgg tcagtattgg tacatcgtta ccagaaatca tcgtctcgct
    48181 tgccgcgtct ctgcacgaac aacgcgattt agccgttggt acagccctcg gctcaaacat
    48241 tatcaatata ttgctgctcc tcggtctggc tgcgctggtt cgtcctttta ccgtccattc
    48301 cgatgttctg cgccgtgaat tacccttaat gttgttggtc agcgtggtgg ccggttccgt
    48361 actctatgac ggacaactta gtcgaagcga tggtatcttt ctcctctttc tggctgtgct
    48421 atggctgctg ttcattgtta aacttgcacg tcaggggact gacagcctga ccagagagca
    48481 gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc
    48541 gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa
    48601 ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagtct
    48661 gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg
    48721 aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat
    48781 aacgccagga gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt
    48841 gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg
    48901 ggtattatta actggcggat ttatcatatg gctggcgatg ttgtactggt tatcgccaat
    48961 actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg ggttttgact
    49021 ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc
    49081 aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag
    49141 ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc gcaacgtttg
    49201 ccagcaccgg tacaccttca tttttcgtcc atcctagtga agccgcgcat ggtgatttag
    49261 gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa
    49321 tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc
    49381 gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag
    49441 aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg
    49501 atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct
    49561 cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata
    49621 cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag
    49681 ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca
    49741 tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa
    49801 gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg
    49861 aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc
    49921 atttactcgg tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca
    49981 aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag
    50041 cgccgacgtt atggcaaaag cagagaacat tcgtctgctg atcctcgacg ttgatggcgt
    50101 actgtcagat ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt
    50161 tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac
    50221 cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta
    50281 tcaggggcag tcaaacaaac tgatcgcctt tagcgatttg ctggaaaaat tggcgattgc
    50341 cccggaaaat gtggcttatg ttggcgatga tctcatcgac tgcccggtaa tggaaaaagt
    50401 gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt
    50461 gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat tactcctggc
    50521 gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt
    50581 atcattgtgc tatcactggc ggttctggtg atgatcggca ttaatatggc cgaaaaagac
    50641 gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac
    50701 acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca acacgttgaa
    50761 tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag
    50821 gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg
    50881 ctctatttat atggacacgt tgaagtcaac gcacttgtgc cagactctca acttcgcaga
    50941 atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc
    51001 gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc
    51061 agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa
    51121 actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc
    51181 cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat
    51241 gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat
    51301 taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga
    51361 cggatacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg
    51421 tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc
    51481 ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc tggtgaaaga
    51541 gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc tggtgccgtc
    51601 gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt
    51661 cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt
    51721 agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg
    51781 tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa
    51841 catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg cgcgccgcgg
    51901 tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct
    51961 gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa
    52021 cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc
    52081 cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat
    52141 tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgata ttaaacgcat
    52201 cattgaacac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga
    52261 aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg
    52321 cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt
    52381 cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg
    52441 aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag
    52501 gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca gcaggcgctg
    52561 gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg
    52621 caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag
    52681 ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc
    52741 ggtgactaca ttgacgacga gctgccggtc taccagggcg aaacgacgca gaccttgcag
    52801 gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct
    52861 acctctatcg tcgatgccgt tgatgaaacc ggttatctga ctgtcccgct ggaagatatt
    52921 ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt ccttaagcgg
    52981 atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc
    53041 caactctccc aattcgataa gaccacgccg tggctggaag aggccagatt gatcattagc
    53101 gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg
    53161 aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg
    53221 cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat
    53281 aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca aatcaaccag
    53341 cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt tatccgcagc
    53401 aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg
    53461 cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg tgaagaatat
    53521 atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata
    53581 tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat
    53641 ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt
    53701 gcactggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag
    53761 ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac
    53821 cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg acccaaccga
    53881 taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact
    53941 gcgcgaattt gttacagcta aatttgccaa acttgagcaa tattttgacc gaatcaacca
    54001 ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt
    54061 aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg
    54121 cttaattgat aagctggcac gccagttgac caaacataaa gataaactga aacaacacta
    54181 attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt
    54241 acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat
    54301 gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag
    54361 ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa
    54421 acaacttagc ctaccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg
    54481 cagtaccggt atcggcaatg gtattgccat tccgcatggc aaacttgaag aagatactct
    54541 gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg ccatcgacaa
    54601 ccaaccggtg gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct
    54661 gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg
    54721 cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag gtactccgga
    54781 tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt
    54841 actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga
    54901 agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg
    54961 aactctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga
    55021 gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact
    55081 actgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact
    55141 gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgattt
    55201 gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca
    55261 cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgaccat
    55321 ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga
    55381 cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga
    55441 taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac
    55501 gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta gctacctgac
    55561 ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc
    55621 agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg
    55681 taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg
    55741 cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat
    55801 gacgaaggca ccgaggctga agccaacagc gttattgcgc tgctgatgtt ggattctgcc
    55861 aaaggacggc agattgaagt tgaagcgacc ggtccacagg aagaggaagc actggccgcc
    55921 gttatcgccc tctttaattc tggttttgat gaagattaat cttcatcact ttgacataca
    55981 actcccttca agctcccccg acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac
    56041 gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa accttaagag
    56101 gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag aaaatatttc
    56161 caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga
    56221 aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg
    56281 atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt ggtcgccggt
    56341 taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat tgcggaaccg
    56401 gttatgtcta cgatgtgatt gccgattttg acggcaagcc ttccattacg ctgaccgaat
    56461 ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta ctgaaacagc
    56521 tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc
    56581 tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac attggtgaaa
    56641 gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag caagagcggt
    56701 tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac
    56761 ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc tggtgttgtt
    56821 gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctac
    56881 gcacgtagcc gtcaggcgtg gagactttga aacgaagtgg attaggtaat acagctgcca
    56941 gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg
    57001 cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga
    57061 tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc agcccttttc
    57121 gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga gaaatcgttg
    57181 aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc tcaatggaag
    57241 cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc accgccagtc
    57301 ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg taacgaaaat
    57361 tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag ggaacaggcg
    57421 caacgctaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca
    57481 aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac
    57541 cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag
    57601 catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg
    57661 acacggcaca tgctccgcgc ccatctcttc cagtacttct gcggtatcga tatcagtacc
    57721 gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat
    57781 aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc
    57841 aacggtgcat tcgctaccaa gactggcaaa attgcttaaa ttcttcgccg cgccaacccc
    57901 ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc
    57961 accgcgcgtt attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcttcgcc
    58021 agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc
    58081 accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata
    58141 gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt
    58201 gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca
    58261 tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt
    58321 gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg tgttggcgca
    58381 gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt
    58441 tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt
    58501 tatcttccca ggcgggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc
    58561 gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt
    58621 tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc atcttctcaa
    58681 acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca
    58741 tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct
    58801 catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga accgaaagcg
    58861 gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt
    58921 tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac
    58981 gagagatatc cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt
    59041 cgcccggttt aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac
    59101 ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca
    59161 gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg
    59221 ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa
    59281 ccgtaatatc gccgcccata tttttcgcca gacgatgaga aacggccaga ccaataccgg
    59341 tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaagattt
    59401 tatccagctc atcctgcgga atgccgatac cagaatcttc cacttcaaaa tgcagcatat
    59461 cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt
    59521 tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg
    59581 gtaatggcag cgtaggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg
    59641 agagattttc cagatcggca aggaagctgg tgaaatcaac cggctgatta tcaagctgga
    59701 ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga
    59761 cggccgaaac atggatagtt ttgagatatt tttcctgctc ggcggtgagt tcggtatcca
    59821 gcagaatgcg gctcaggccg acgataccgt tcagcggtgt acgcaattcg tgactgatgg
    59881 tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga taccgcttac
    59941 gctcggtaat gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt
    60001 acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt
    60061 cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg acttttgcgg
    60121 cggcttccgg tgagtaaaca tcagcaggtt tcagatgaac cagttgtttt tcgctttttc
    60181 cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa
    60241 aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct
    60301 gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca
    60361 gttctgcttc cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt
    60421 ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc
    60481 gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg
    60541 cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct
    60601 gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca
    60661 gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact
    60721 gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat
    60781 acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac
    60841 ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt
    60901 cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca gcggagcaag
    60961 cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg
    61021 gcgaatcatt tctccggcag tgagcgtata atcctccagt tcaataccgt tcaaacgccc
    61081 cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat
    61141 gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc
    61201 gccaggcagg ccgacaatta agtgggaaca caccttcagc ccgcgctgac gtgccagctg
    61261 ggttgtacgc tgataacagg caaaatcatg gccgcggttg atgcgatgca gtgttttgtc
    61321 gtgggcggtt tgtagcccca gctccagcca cacttcgtaa ccctggtcct tatattcgca
    61381 aagcagatcc agcaccgcat ccggcacgca gtccgggcgg gtaccaacac acaaaccgac
    61441 aatattggcc tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa
    61501 ggtgctggta tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc
    61561 ctggtgcgcc agttgctcgg caatggaacg atgctgctgc gcttcatcgg caaacgaggc
    61621 aacattacag aatgtgcagc caccacgccc gatggtaccg tcacggttag ggcagctaaa
    61681 accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc
    61741 aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc
    61801 caaagcctgc catttttagc tcaattcggc gatgacctag atcaatcgtc ccggcctgct
    61861 tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca cataaatcac
    61921 ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca aaaacagtag
    61981 catgaaacgt cattaccaat taaggcagta taaaatgctg gttttctcgt cagttcaagg
    62041 caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct ctaacgactc
    62101 tccttttagc cttaaagata aaatctattt taatttcagc catttaataa agaattttgc
    62161 gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc tttcccgata
    62221 agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat ttatgcagta
    62281 attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc
    62341 gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct ttctgtgccc gtgcgcagcg
    62401 gttcggaagt ggggttcccg cagagcctgg gggaggttca cgatatgttg tacgataaat
    62461 cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc gaacctagcc
    62521 acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt ggcgcgattc
    62581 tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt
    62641 ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa aaactacgct gtcgggatgc
    62701 tcttcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt gaagaagaac
    62761 tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc
    62821 tgggtgaaat cgccctctcc tctctgccac gcattgagca aatttttgtg aacgccccgg
    62881 caggctggcg tccacgcgat atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa
    62941 agcgtctcga agccgacaaa gacttctacg tctgtagcct gtcgaatctg gtgaacatct
    63001 ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt gcggacctgc
    63061 gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc gtaccgcgct
    63121 ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac accatcaccg
    63181 gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctg atccctgacc
    63241 tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga ctccagttcc atggataaca
    63301 tgctggaact gctgctggca ggcgggatgg atatcatccg cgccatgcgc ctgttagtgc
    63361 cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc tttgacttta
    63421 actccatgca tatggagccg tgggatggtc cggcgggcat cgtgatgtcc gatggtcgct
    63481 tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc acgctacgtc attaccaaag
    63541 acaagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct gacgaagtgg
    63601 tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat cgacactcgc agtgggcgta
    63661 ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccacccttat aaagagtgga
    63721 tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta
    63781 gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac tacagcgcgg
    63841 aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga
    63901 tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt tacgactact
    63961 tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg
    64021 ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc
    64081 aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc aaacagctca
    64141 cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt gacgtcacta
    64201 aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa agccgaaaaa atggtacgta
    64261 gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc taaagatcgc ctgccggttc
    64321 cggctccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg
    64381 atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc gccgtgttgc
    64441 tgggtttcgg cgcgacggct atttatccgt accttgccta tgaaacgctg ggccgcctgg
    64501 tagacaccca cgcgattgcc aaagattatc gtaccgtgat gctcaactac cgtaacggca
    64561 tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc gcctcttacc
    64621 gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc ctgtgcttcc
    64681 agggcgcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag gatctgctga
    64741 atctgtcgaa acgtgcctgg ctggcgcgca agccgattag ccagggcggc ctgctgaaat
    64801 acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg ctgcaacagg
    64861 cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt aatgagcgtc
    64921 cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg
    64981 ctgatgttga accggcaagc gaactgttta aacgctttga taccgccgcg atgtctatcg
    65041 gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaatagc atcggcggta
    65101 actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta tggcactaac aaagtgtcgc
    65161 gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc ggcgtatctg gtcaatgccg
    65221 acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt cagttgccgg
    65281 gcgacaaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga gtgacgctga
    65341 tctccccgcc gccgcaccac gatatctact ctatcgaaga cttagcgcag cttattttcg
    65401 acctcaagca ggttaacccg aaagcgatga tctccgttaa gctggtgtcc gaaccaggcg
    65461 ttggcaccat cgcgactggc gtggcgaaag cttatgcgga cttgatcacc atcgcgggct
    65521 atgacggcgg caccggcgca agcccgctgt cgtcagtgaa atatgcaggc tgcccgtggg
    65581 aactcggtct ggtggaaacc caacaggcgc tggttgccaa cggcttgcgt cataaaattc
    65641 gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat catcaaagcg gcgattctcg
    65701 gcgcagaaag cttcggcttc ggcactggcc caatggtggc gctcggctgt aaatatctgc
    65761 gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac tcaggatgac aaactgcgta
    65821 agaatcacta tcacggcctg ccgttcaagg tgactaatta ctttgagttt atcgcccgtg
    65881 aaacccgcga gctgatggca cagctgggag taacgcgtct ggtggatctg attggtcgca
    65941 ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg gcgctgtcga
    66001 agctgctgga gactgccgag ccacatgcag gtaagtcact ctactgcact gaaaacaacc
    66061 cgccgtttga taacggccta ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg
    66121 atgaacgcca gagcaaaacc ttctggttcg atatccgcaa caccgaccgt tctgtcggcg
    66181 cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca gccgatccta
    66241 tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtgtggaac gcgggcggcg
    66301 tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg gcgggcggct
    66361 taatcgccat tcatcctccg gttggttccg ccttccgcag ccatgaagca agcattatcg
    66421 gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg
    66481 aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg
    66541 gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc gttaacttcg
    66601 gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat ttccgcaaac
    66661 gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga tctggcgatc catgaagagc
    66721 atctgcgcgg tcttatcacc gagcatgttc agcataccgg ttctcagcgc ggtgaagaga
    66781 ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct ggttaaaccg aagtccagtg
    66841 atgtaaaagc actgctgggt caccgtagtc gtagcgcagc agagttgcgc gtgcaggcgc
    66901 agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg cagcgcgttg
    66961 atccgccaaa gaaaccgctg aagatccgta aaattgagtt tgttgaaatt tacgagccgt
    67021 tttccgaagg ccaggccaaa gcgcaggctg accgctgtct gtcgtgcggc aacccatact
    67081 gcgagtggaa atgcccggta cacaactaca tcccaaactg gctgaagctc gccaacgagg
    67141 ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa cacactgccg gaagtatgcg
    67201 gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg caccctgaac gatgagttcg
    67261 gtgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc gaaatgggct
    67321 ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt atcggtgcag
    67381 gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa gccgttgtct
    67441 tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc ttcaagctgg
    67501 aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac
    67561 tcaataccga agtgggccgc gacgtacagc tggacgatct gctgagtgat tacgatgccg
    67621 tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg
    67681 atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta atgggctttg
    67741 gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggta gtacttggcg
    67801 gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg ccagggggca aagcacgtta
    67861 cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg
    67921 cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt gaagtgaacg
    67981 gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa ccggatgcca
    68041 aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg gcagatgcgg
    68101 tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa cacagcgtcg
    68161 agctggattc acaaggccgc atcatcgctc cggaaggcaa cgacaacgct ttccagacca
    68221 gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg gtcgttaccg
    68281 ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa ctggctggaa gtttaagctc
    68341 tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt ctttctggaa
    68401 atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt gttaccggta
    68461 gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag ggacagataa
    68521 tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac ggcattattc
    68581 atctggtcga tagcgcccga acggaaacgg tacgtagcgt taacgcgctg atgaccgcga
    68641 cgtactggga aattggccga cggattgtcg aatttgaaca aggtggtgag gccagagcag
    68701 cttatggtgc acagttgatt gatcggctat cgaaggattt aagtcgacgg tataaacgtg
    68761 gggtctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt caacatgttg
    68821 aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag acaccatctg
    68881 cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga ggctgtttga
    68941 tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca tgcaaataaa
    69001 cgaagg
//
LOCUS       DADWTH010000006        68192 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.006, whole genome shotgun sequence.
ACCESSION   DADWTH010000006 DADWTH010000000
VERSION     DADWTH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.006"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..247
                     /locus_tag="JNY02_000397"
     CDS             <1..247
                     /locus_tag="JNY02_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBB9432067.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNY02_000398"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNY02_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBB9432068.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNY02_000399"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNY02_000399"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBB9432069.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="JNY02_000400"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="JNY02_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBB9432070.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="JNY02_000401"
     CDS             complement(3258..3542)
                     /locus_tag="JNY02_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432071.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNY02_000402"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNY02_000402"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBB9432072.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="JNY02_000403"
     CDS             complement(4443..5651)
                     /locus_tag="JNY02_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9432073.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAAAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="JNY02_000404"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="JNY02_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBB9432074.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="JNY02_000405"
     CDS             complement(6446..8131)
                     /locus_tag="JNY02_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBB9432075.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="JNY02_000406"
     CDS             8401..8778
                     /locus_tag="JNY02_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBB9432076.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNY02_000407"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNY02_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBB9432077.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="JNY02_000408"
     CDS             9225..9512
                     /locus_tag="JNY02_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBB9432078.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="JNY02_000409"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="JNY02_000409"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBB9432079.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="JNY02_000410"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="JNY02_000410"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBB9432080.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="JNY02_000411"
     CDS             11269..11745
                     /locus_tag="JNY02_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBB9432081.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="JNY02_000412"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="JNY02_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBB9432082.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRVKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="JNY02_000413"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="JNY02_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBB9432083.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="JNY02_000414"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="JNY02_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBB9432084.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="JNY02_000415"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="JNY02_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBB9432085.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="JNY02_000416"
     CDS             16302..16790
                     /locus_tag="JNY02_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBB9432086.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="JNY02_000417"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="JNY02_000417"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBB9432087.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JNY02_000418"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JNY02_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBB9432088.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JNY02_000419"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JNY02_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBB9432089.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JNY02_000420"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JNY02_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBB9432090.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JNY02_000421"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JNY02_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB9432091.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JNY02_000422"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JNY02_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBB9432092.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="JNY02_000423"
     CDS             complement(22242..22757)
                     /locus_tag="JNY02_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB9432093.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="JNY02_000424"
     CDS             23406..25175
                     /locus_tag="JNY02_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBB9432094.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSIGARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="JNY02_000425"
     CDS             25386..25709
                     /locus_tag="JNY02_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBB9432095.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="JNY02_000426"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="JNY02_000426"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBB9432096.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="JNY02_000427"
     CDS             complement(26533..27546)
                     /locus_tag="JNY02_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBB9432097.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="JNY02_000428"
     CDS             complement(27645..29075)
                     /locus_tag="JNY02_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBB9432098.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JNY02_000429"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JNY02_000429"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBB9432099.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JNY02_000430"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JNY02_000430"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBB9432100.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JNY02_000431"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JNY02_000431"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBB9432101.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPNLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JNY02_000432"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JNY02_000432"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBB9432102.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JNY02_000433"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JNY02_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBB9432103.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JNY02_000434"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JNY02_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBB9432104.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYAEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37227..38885
                     /locus_tag="JNY02_000435"
     CDS             37227..38885
                     /locus_tag="JNY02_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBB9432105.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38882..39838)
                     /locus_tag="JNY02_000436"
     CDS             complement(38882..39838)
                     /locus_tag="JNY02_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBB9432106.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39989..41104
                     /gene="macA"
                     /locus_tag="JNY02_000437"
     CDS             39989..41104
                     /gene="macA"
                     /locus_tag="JNY02_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBB9432107.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41101..43047
                     /gene="macB"
                     /locus_tag="JNY02_000438"
     CDS             41101..43047
                     /gene="macB"
                     /locus_tag="JNY02_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBB9432108.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="JNY02_000439"
     CDS             complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="JNY02_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBB9432109.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43667..43987
                     /gene="clpS"
                     /locus_tag="JNY02_000440"
     CDS             43667..43987
                     /gene="clpS"
                     /locus_tag="JNY02_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBB9432110.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44018..46294
                     /gene="clpA"
                     /locus_tag="JNY02_000441"
     CDS             44018..46294
                     /gene="clpA"
                     /locus_tag="JNY02_000441"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBB9432111.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46697..46784)
                     /locus_tag="JNY02_000442"
     tRNA            complement(46697..46784)
                     /locus_tag="JNY02_000442"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46748..46750),aa:Ser,seq:gga)
     gene            complement(47038..47256)
                     /gene="infA"
                     /locus_tag="JNY02_000443"
     CDS             complement(47038..47256)
                     /gene="infA"
                     /locus_tag="JNY02_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBB9432112.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47541..48245)
                     /gene="aat"
                     /locus_tag="JNY02_000444"
     CDS             complement(47541..48245)
                     /gene="aat"
                     /locus_tag="JNY02_000444"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBB9432113.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="JNY02_000445"
     CDS             complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="JNY02_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBB9432114.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="JNY02_000446"
     CDS             complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="JNY02_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBB9432115.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="JNY02_000447"
     CDS             complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="JNY02_000447"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBB9432116.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53408..53902
                     /gene="lrp"
                     /locus_tag="JNY02_000448"
     CDS             53408..53902
                     /gene="lrp"
                     /locus_tag="JNY02_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBB9432117.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54037..58143
                     /gene="ftsK"
                     /locus_tag="JNY02_000449"
     CDS             54037..58143
                     /gene="ftsK"
                     /locus_tag="JNY02_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBB9432118.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAVDPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58298..58909
                     /gene="lolA"
                     /locus_tag="JNY02_000450"
     CDS             58298..58909
                     /gene="lolA"
                     /locus_tag="JNY02_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBB9432119.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDEQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58920..60263
                     /gene="rarA"
                     /locus_tag="JNY02_000451"
                     /gene_synonym="mgsA"
     CDS             58920..60263
                     /gene="rarA"
                     /locus_tag="JNY02_000451"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBB9432120.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60354..61646
                     /gene="serS"
                     /locus_tag="JNY02_000452"
     CDS             60354..61646
                     /gene="serS"
                     /locus_tag="JNY02_000452"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBB9432121.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61886..64330
                     /gene="dmsA"
                     /locus_tag="JNY02_000453"
                     /pseudo
     CDS             61886..64330
                     /gene="dmsA"
                     /locus_tag="JNY02_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64341..64958
                     /gene="dmsB"
                     /locus_tag="JNY02_000454"
     CDS             64341..64958
                     /gene="dmsB"
                     /locus_tag="JNY02_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBB9432122.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64960..65823
                     /gene="dmsC"
                     /locus_tag="JNY02_000455"
     CDS             64960..65823
                     /gene="dmsC"
                     /locus_tag="JNY02_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBB9432123.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65859..66485)
                     /locus_tag="JNY02_000456"
     CDS             complement(65859..66485)
                     /locus_tag="JNY02_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBB9432124.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66800..67948
                     /locus_tag="JNY02_000457"
     CDS             66800..67948
                     /locus_tag="JNY02_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9432125.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGSVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16937 a        16864 c        17863 g        16528 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttacactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattct ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
     3721 tgcgacgacc gcgctgccgg catttttcat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcactac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatact
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcggc ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgccctg gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaagc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggtga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg tgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tctgccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggtaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgttacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgt gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 agacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaacaattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg ttttattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atcggtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gtgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gtgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaaca caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtt aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaactaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggt taagatgctt aacgcgggga taaaagggat
    34681 ttttcatgta actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 caggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcggca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaatcac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatatg
    36841 gtcgttttat agggtcgtcg taaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    36901 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    36961 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc
    37021 tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg tttttgcggg
    37081 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca attgccatgc
    37141 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    37201 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    37261 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
    37321 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    37381 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    37441 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    37501 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    37561 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    37621 ataaagacgg acatccgatt gatgttgagg atattaacga tcaggcacgc catctggtgc
    37681 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    37741 cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    37801 agttgtcctc acatccgcaa aatctctctg atggacagat tcgtcaggga ctttccgcaa
    37861 tggtgcagtt gcttgagcat tatttctctg agcagggggc tggacaggcg cgatatcgtt
    37921 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    37981 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    38041 ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcgcgtccg ttattgctaa
    38101 ttgaagatcc agaaacccgc ctgcatccga ttatgctttc cgttgcctgg catctgttga
    38161 atcttttgcc tttacagcgt attgctacca ccaactctgg cgagctgctt tcgttaactc
    38221 cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg
    38281 ggccaagtgg cttgagcacc gaagacagcc gccgcatctc ttttcacatt cgttttaatc
    38341 gtccgtcatc gctgtttgct cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    38401 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    38461 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    38521 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    38581 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    38641 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    38701 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    38761 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
    38821 taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    38881 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    38941 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    39001 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc cagaacgcgt
    39061 tgtaatcagc atggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    39121 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    39181 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    39241 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    39301 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgga
    39361 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
    39421 actgctcacc gtttttgcct tccagtttag ccagttgcag cccgggggta ttcaaatata
    39481 agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa cgtaacgcct
    39541 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    39601 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    39661 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    39721 agcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    39781 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    39841 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    39901 tgtgattact aacgtaaatt ttaggatttt gttgatattt cgttgaagtt aatgacccgg
    39961 attggcatgt ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    40021 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    40081 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    40141 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca
    40201 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    40261 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
    40321 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    40381 tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag gatctcgata ccgccgcgac
    40441 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
    40501 ggcctctctc gatacggcta aaaccaatct cgattacacc cgtatcgttg ccccgatggg
    40561 cggggaagtg acgcaaatca ccactctgca aggccagacg gtgattgccg cacagcaagc
    40621 accgaacatt ctgacgctgg cagatatgag cactatgctg gtaaaagcgc aggtttctga
    40681 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
    40741 actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa aggtaaacga
    40801 cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttgc tgcggctgga
    40861 tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgttga cgatccctct
    40921 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    40981 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    41041 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    41101 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    41161 gttgaggtgc tgaagggcat cagcctcaat atttatgcgg gtgagatggt cgcgattgtt
    41221 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
    41281 accagcggca cctatcgcgt agccggtcag gatattgcca cgctggacgc cgatgcgctg
    41341 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
    41401 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    41461 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg cacagagtat
    41521 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    41581 aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag ccattctggc
    41641 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
    41701 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    41761 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    41821 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    41881 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    41941 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    42001 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    42061 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
    42121 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    42181 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    42241 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    42301 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    42361 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    42421 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    42481 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
    42541 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
    42601 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    42661 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    42721 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    42781 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    42841 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
    42901 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
    42961 tcgacggcca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
    43021 ccagtagatg ctctggcacg ggagtaattt ttgagataaa aatgccagcc gatcgggctg
    43081 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    43141 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    43201 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    43261 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaacctttgg cattgttgaa
    43321 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    43381 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    43441 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
    43501 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    43561 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    43621 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    43681 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    43741 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    43801 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    43861 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    43921 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    43981 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    44041 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    44101 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    44161 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    44221 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    44281 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
    44341 tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    44401 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    44461 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    44521 tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa tcgacccact
    44581 aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    44641 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc
    44701 ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga tttactctct
    44761 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
    44821 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    44881 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
    44941 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    45001 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    45061 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    45121 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
    45181 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
    45241 cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    45301 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
    45361 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggccaggata aagccattga
    45421 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    45481 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
    45541 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
    45601 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
    45661 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
    45721 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    45781 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg
    45841 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
    45901 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    45961 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    46021 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    46081 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    46141 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    46201 cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    46261 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtgaag
    46321 tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg tgaaaaccct
    46381 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
    46441 agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa acgtgtagta
    46501 tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg
    46561 aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    46621 accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg
    46681 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    46741 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
    46801 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    46861 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    46921 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    46981 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    47041 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    47101 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    47161 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    47221 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    47281 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    47341 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    47401 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    47461 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    47521 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    47581 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    47641 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    47701 ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    47761 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    47821 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    47881 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    47941 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    48001 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    48061 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    48121 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    48181 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    48241 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    48301 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    48361 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    48421 tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg atctggcttt
    48481 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    48541 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    48601 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    48661 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    48721 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    48781 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    48841 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    48901 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    48961 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    49021 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    49081 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    49141 cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa
    49201 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    49261 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    49321 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    49381 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    49441 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    49501 taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    49561 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    49621 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    49681 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    49741 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    49801 cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    49861 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    49921 gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    49981 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    50041 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    50101 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    50161 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    50221 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    50281 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    50341 caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    50401 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgca
    50461 attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    50521 ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    50581 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca accaacactg
    50641 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    50701 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct aattccgcct
    50761 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    50821 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    50881 ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta tcgtagtgac
    50941 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    51001 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    51061 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    51121 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    51181 caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc agcgccataa
    51241 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    51301 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcagatagc
    51361 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    51421 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    51481 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    51541 cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    51601 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    51661 tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    51721 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    51781 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    51841 aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    51901 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt
    51961 accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    52021 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    52081 gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac
    52141 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    52201 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    52261 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    52321 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    52381 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    52441 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    52501 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    52561 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    52621 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta ataacggacc
    52681 ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    52741 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    52801 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    52861 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    52921 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    52981 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    53041 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt
    53101 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    53161 cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg aaatctacgt
    53221 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    53281 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    53341 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    53401 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    53461 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    53521 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    53581 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    53641 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    53701 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    53761 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    53821 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    53881 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    53941 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    54001 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    54061 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    54121 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    54181 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    54241 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    54301 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ctagcgacga atacattgat
    54361 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    54421 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    54481 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    54541 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    54601 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    54661 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    54721 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    54781 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    54841 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgtcgac
    54901 ccggacgacg tcctattttt gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    54961 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    55021 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    55081 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    55141 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    55201 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    55261 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    55321 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    55381 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccatt
    55441 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    55501 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    55561 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    55621 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    55681 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    55741 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    55801 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    55861 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    55921 actcgtcgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    55981 aaagcccgtg aagcccagcg caatcagtat gattctggcg atcagtataa cgatgatgaa
    56041 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    56101 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    56161 gaggctgaac tggctcgtca gtttacgcaa actcaacaac aacgttattc cggcgaacaa
    56221 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    56281 ctggatgatg gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg
    56341 caacaaccgg ttgcaccaca gcagcagtat cagcagccac aacagccggt tgcgccacag
    56401 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    56461 caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg
    56521 cagtatcaac agccgcaaca accggttgca ccgcagccgc agtatcagca gccacaacag
    56581 ccagttgcgc cacagccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    56641 agccgtccgt tgcacaaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    56701 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg tctggtggaa
    56761 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtta attactctcc ggggccagtt
    56821 atcactcgct ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg
    56881 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    56941 ggcaaaccct atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc
    57001 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    57061 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    57121 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    57181 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    57241 tcggtttatg aaggcattcc gcacctgtta acggaagtcg ttactgatat gaaagatgcc
    57301 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    57361 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    57421 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    57481 ctgaaaaaag aaccatacat tgtcgtgttg gttgacgaat ttgccgacct gatgatgacg
    57541 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    57601 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    57661 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    57721 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    57781 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    57841 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    57901 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    57961 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    58021 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    58081 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    58141 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    58201 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    58261 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ctatcacctg
    58321 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    58381 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    58441 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    58501 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    58561 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    58621 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    58681 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    58741 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    58801 gcagcgcagc agttatcagc tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    58861 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    58921 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    58981 ggccagaaaa tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt
    59041 tgccgcgcgc tatcgaagcc gggcatttgc attctatgat cctctggggg ccaccgggta
    59101 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    59161 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    59221 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    59281 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    59341 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc
    59401 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    59461 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgatacacga cgtgccattg
    59521 cagaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    59581 tggccgaagt ctatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    59641 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctaatttccg
    59701 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    59761 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    59821 aagacgtcgg taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    59881 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    59941 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    60001 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    60061 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    60121 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    60181 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    60241 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    60301 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    60361 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    60421 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    60481 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    60541 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    60601 atgcagcaaa agccgagcta gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    60661 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    60721 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    60781 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    60841 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    60901 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    60961 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    61021 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    61081 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    61141 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    61201 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    61261 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    61321 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    61381 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    61441 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    61501 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    61561 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    61621 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgccttta
    61681 gggcgctttt tttgtctccc tttgataccg aacaataatt actcctcact tacacgtaat
    61741 actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg atgtatacaa
    61801 gcctatatag cgagctgcta tagaaataat tacacaatac ggtttgttac tggaatcaat
    61861 cgtgatcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg tattggctgc
    61921 tgaggagagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg caatggccag
    61981 cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg ccattccaac
    62041 aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta gtcgctgccc
    62101 gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc gaaacggaca ataccggcga
    62161 tgacaattac gacggcctgc actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg
    62221 ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga
    62281 aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga ccaatatgca
    62341 gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca ccggtacgct
    62401 gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa
    62461 ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa ttgcggaagg
    62521 tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata tcgaaaacag
    62581 taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt
    62641 gacttactat cttgaacagg cacgccagaa atctaatgct cgcataatca tcatcgatcc
    62701 gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc gtccaggaac
    62761 agatgccgca ctggttaacg gtctggcgta cgtcatgatc actgaaaacc tggtggatca
    62821 ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc
    62881 gaaaaatggt cattataaag cttatattct gggtgaaggg ccagatggcg tggctaaaac
    62941 accggaatag gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca aactggctcg
    63001 tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc agcgtcacgc
    63061 taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg gtaacgttgg
    63121 tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat
    63181 gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga ccgatgccat
    63241 tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga
    63301 tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc agcattctga
    63361 aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga ttgtggttat
    63421 cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg actgcaccgc
    63481 ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt acgtgatttt
    63541 caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa tgaccagcga
    63601 actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg
    63661 gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa cgtttgaaga
    63721 gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa
    63781 agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca aaattgagat
    63841 ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag gcgatgtgat
    63901 cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc tgaacaaaca
    63961 gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa cttacggcaa
    64021 cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca
    64081 aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg gtgaggttca
    64141 tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc
    64201 ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg tactgaccac
    64261 tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg ttcaggttga
    64321 aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat tccagccgtt
    64381 gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg acgccagaag
    64441 tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat aacggtgtct
    64501 ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc gaagatccgg
    64561 cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt tttgttgtgg
    64621 tcgatgaaga tgtatgcatt ggctgccgtt actgtcatat ggcttgcccg tatggcgcac
    64681 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
    64741 cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc
    64801 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
    64861 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgacagggg
    64921 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
    64981 tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg
    65041 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
    65101 atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct ccatcttggt
    65161 tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
    65221 atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct ggcaatgctg
    65281 aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc
    65341 gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
    65401 agcatctgga cgccgatggg cttcttcctg acgatgttca tgggcggccc gttactgggt
    65461 tacctgttgc tgagtctggc tggcgtcgat ggctgggcga tgcgtctgct gccagccatt
    65521 tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca
    65581 actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg
    65641 tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa
    65701 ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa
    65761 ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc
    65821 taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg
    65881 tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt
    65941 ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc
    66001 aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag
    66061 gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc
    66121 gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc
    66181 gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca
    66241 atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg
    66301 gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc
    66361 gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca
    66421 gcctggtgat caacaagcaa aacggcagca tcatttttat caagacgaac atacggtttg
    66481 gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca
    66541 ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg
    66601 aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct
    66661 cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc
    66721 gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac
    66781 ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt tgctgtctgg
    66841 cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc
    66901 ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa
    66961 ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta
    67021 ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt
    67081 ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt
    67141 tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc
    67201 gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc
    67261 aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc
    67321 gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac
    67381 ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaag
    67441 cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa
    67501 tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc
    67561 gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt
    67621 cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat
    67681 cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt
    67741 tgaacatcat caactggtgg cgatgaacca agccttactg ttgagctata ccgtgggaag
    67801 tctgcttggc ccgtcattta ccgctatgct aatgcagaat ttctccgata atttattgtt
    67861 tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgcttcgca acgccggtca
    67921 tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt
    67981 cacactctgc ttttttgttt cttctatctg acttgctttt ttccaaattt tattcgttta
    68041 aaaataaaat gtgcagcagg ttataatttt gcatttcgct atttccgcac ttcttatttg
    68101 ccgcgcataa tccctcgttt tacagatgcc cctttaattt tggcgaagga tttgtctatg
    68161 gctgggaatg ttcaggaagt aagcgcccca tc
//
LOCUS       DADWTH010000007        67095 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.007, whole genome shotgun sequence.
ACCESSION   DADWTH010000007 DADWTH010000000
VERSION     DADWTH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.007"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="JNY02_000458"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="JNY02_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBB9432126.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="JNY02_000459"
     CDS             complement(1150..1236)
                     /locus_tag="JNY02_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBB9432127.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="JNY02_000460"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="JNY02_000460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBB9432128.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="JNY02_000461"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="JNY02_000461"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBB9432129.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="JNY02_000462"
     CDS             3585..3629
                     /locus_tag="JNY02_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432130.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="JNY02_000463"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="JNY02_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBB9432131.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="JNY02_000464"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="JNY02_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBB9432132.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="JNY02_000465"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="JNY02_000465"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBB9432133.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="JNY02_000466"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="JNY02_000466"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBB9432134.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="JNY02_000467"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="JNY02_000467"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBB9432135.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKSGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="JNY02_000468"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="JNY02_000468"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBB9432136.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="JNY02_000469"
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="JNY02_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBB9432137.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="JNY02_000470"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="JNY02_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBB9432138.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /gene="sgrR"
                     /locus_tag="JNY02_000471"
     CDS             11798..13453
                     /gene="sgrR"
                     /locus_tag="JNY02_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBB9432139.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="JNY02_000472"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="JNY02_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBB9432140.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="JNY02_000473"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="JNY02_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBB9432141.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="JNY02_000474"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="JNY02_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBB9432142.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIVWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="JNY02_000475"
     CDS             complement(16982..17746)
                     /locus_tag="JNY02_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBB9432143.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="JNY02_000476"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="JNY02_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBB9432144.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20748
                     /gene="araB"
                     /locus_tag="JNY02_000477"
                     /pseudo
     CDS             19049..20748
                     /gene="araB"
                     /locus_tag="JNY02_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="JNY02_000478"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="JNY02_000478"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBB9432145.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="JNY02_000479"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="JNY02_000479"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBB9432146.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSDVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="JNY02_000480"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="JNY02_000480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBB9432147.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="JNY02_000481"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="JNY02_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBB9432148.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="JNY02_000482"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="JNY02_000482"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBB9432149.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="JNY02_000483"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="JNY02_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBB9432150.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="JNY02_000484"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="JNY02_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBB9432151.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTYVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="JNY02_000485"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="JNY02_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBB9432152.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKLSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="JNY02_000486"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="JNY02_000486"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBB9432153.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGITEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="JNY02_000487"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="JNY02_000487"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBB9432154.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="JNY02_000488"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="JNY02_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBB9432155.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="JNY02_000489"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="JNY02_000489"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBB9432156.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="JNY02_000490"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="JNY02_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBB9432157.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="JNY02_000491"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="JNY02_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBB9432158.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="JNY02_000492"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="JNY02_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBB9432159.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="JNY02_000493"
     CDS             complement(40421..41815)
                     /locus_tag="JNY02_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9432160.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="JNY02_000494"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="JNY02_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBB9432161.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43443)
                     /locus_tag="JNY02_000495"
                     /pseudo
     CDS             complement(42158..43443)
                     /locus_tag="JNY02_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(43494..44435)
                     /locus_tag="JNY02_000496"
     CDS             complement(43494..44435)
                     /locus_tag="JNY02_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBB9432162.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQTRQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVRISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(44450..45220)
                     /locus_tag="JNY02_000497"
     CDS             complement(44450..45220)
                     /locus_tag="JNY02_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBB9432163.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45691..47205
                     /gene="caiT"
                     /locus_tag="JNY02_000498"
     CDS             45691..47205
                     /gene="caiT"
                     /locus_tag="JNY02_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBB9432164.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47236..48378
                     /gene="caiA"
                     /locus_tag="JNY02_000499"
     CDS             47236..48378
                     /gene="caiA"
                     /locus_tag="JNY02_000499"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBB9432165.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48507..49724
                     /gene="caiB"
                     /locus_tag="JNY02_000500"
     CDS             48507..49724
                     /gene="caiB"
                     /locus_tag="JNY02_000500"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBB9432166.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49798..51351
                     /gene="caiC"
                     /locus_tag="JNY02_000501"
     CDS             49798..51351
                     /gene="caiC"
                     /locus_tag="JNY02_000501"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBB9432167.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51460..52245
                     /gene="caiD"
                     /locus_tag="JNY02_000502"
     CDS             51460..52245
                     /gene="caiD"
                     /locus_tag="JNY02_000502"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBB9432168.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52251..52841
                     /gene="caiE"
                     /locus_tag="JNY02_000503"
     CDS             52251..52841
                     /gene="caiE"
                     /locus_tag="JNY02_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBB9432169.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52927..53322)
                     /gene="caiF"
                     /locus_tag="JNY02_000504"
     CDS             complement(52927..53322)
                     /gene="caiF"
                     /locus_tag="JNY02_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBB9432170.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53580..56801)
                     /gene="carB"
                     /locus_tag="JNY02_000505"
     CDS             complement(53580..56801)
                     /gene="carB"
                     /locus_tag="JNY02_000505"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBB9432171.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56819..57967)
                     /gene="carA"
                     /locus_tag="JNY02_000506"
     CDS             complement(56819..57967)
                     /gene="carA"
                     /locus_tag="JNY02_000506"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBB9432172.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58423..59244)
                     /gene="dapB"
                     /locus_tag="JNY02_000507"
     CDS             complement(58423..59244)
                     /gene="dapB"
                     /locus_tag="JNY02_000507"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBB9432173.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59411..60325)
                     /gene="rihC"
                     /locus_tag="JNY02_000508"
     CDS             complement(59411..60325)
                     /gene="rihC"
                     /locus_tag="JNY02_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBB9432174.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60391..61341)
                     /gene="ispH"
                     /locus_tag="JNY02_000509"
                     /gene_synonym="lytB"
     CDS             complement(60391..61341)
                     /gene="ispH"
                     /locus_tag="JNY02_000509"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBB9432175.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61343..61792)
                     /gene="fkpB"
                     /locus_tag="JNY02_000510"
     CDS             complement(61343..61792)
                     /gene="fkpB"
                     /locus_tag="JNY02_000510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB9432176.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61879..62373)
                     /gene="lspA"
                     /locus_tag="JNY02_000511"
     CDS             complement(61879..62373)
                     /gene="lspA"
                     /locus_tag="JNY02_000511"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBB9432177.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62373..65189)
                     /gene="ileS"
                     /locus_tag="JNY02_000512"
     CDS             complement(62373..65189)
                     /gene="ileS"
                     /locus_tag="JNY02_000512"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBB9432178.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGNGEKRKFA"
     gene            complement(65232..66173)
                     /gene="ribF"
                     /locus_tag="JNY02_000513"
     CDS             complement(65232..66173)
                     /gene="ribF"
                     /locus_tag="JNY02_000513"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBB9432179.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66181..66399)
                     /locus_tag="JNY02_000514"
     CDS             complement(66181..66399)
                     /locus_tag="JNY02_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBB9432180.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66502..66765
                     /gene="rpsT"
                     /locus_tag="JNY02_000515"
     CDS             66502..66765
                     /gene="rpsT"
                     /locus_tag="JNY02_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBB9432181.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16280 a        18256 c        17271 g        15288 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggcttagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcaacgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa agcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaatgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatgttttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattattt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaat ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgcgatt gcgtcttatg aagcaaaaca acctgcgttt atgaattagc ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaac ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcatctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca gccggtgatc tgatgctaaa aggtccacag ggcgaggaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccgactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggcg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccacctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgtctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatgcg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 gacgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctgcaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga ctcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttagaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gacgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgcctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga aggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggttcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac gttatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cctggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 accgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagcg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacct acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccgc aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agctctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattacc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt cttgcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggta gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ctgctctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtgactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tagcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacggggcgg aatcggcgaa
    43261 acctggaata atgtgttcca gactgtgggc atagagacgc ccgccagtaa cgttcttggc
    43321 acccgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    43381 tgcggcaacc gaaccggcaa gccctgcacc gacgatgatg gcgtcaaaga tatcttcgga
    43441 cataagagct ccctgtcagc ggcagagccg taatgcagcc ctgccagagt ggatcaacgc
    43501 gctaaagctg cggtcagtgc cggaaggatc ttcacggcat cgccaacaat gccgtaatcc
    43561 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgacgcg
    43621 ttagcgccaa ccatgtgctg gatctgcccg gagatcctca ccgccaggta cagttcaggt
    43681 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttct
    43741 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagt
    43801 gcaatgttct ctttgctgcc aatgccgcga ccgacgctga ccaccagacg ggctttgtcg
    43861 agatcgacgc tgttgctctg gcgcgtctgg gttgccgtgc gggtaatcgc cacagccgga
    43921 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    43981 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    44041 gccagaccgc cgtaaaccat gtgtttcact gttgctttac cgtcctgtac gctgacggtg
    44101 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    44161 ccgcgacggg tgtttggcaa cagcaccaga ccgtcagcgc cgtgctggcg aatagtgtca
    44221 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    44281 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggca
    44341 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agagggggta
    44401 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    44461 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    44521 gctgacgctc gcgctgtttc ggcgcggcaa cctgttgttc agaccaggcg tccacggcgt
    44581 taaaaccaat atccgccgcc gaccatacct ggactggctt tttcgccgcg ccgagaatgg
    44641 ctttcatcga aggaatttgt ggggagttga tatcagtgga aacagcaaca accgcaggca
    44701 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    44761 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    44821 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcagat
    44881 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    44941 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt
    45001 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggctgtc acctgcgcct
    45061 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttgagatcg tattggctta
    45121 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    45181 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    45241 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    45301 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    45361 gttaagttcc tcaccaatat tgaaaacatg gcgtagcaaa aagaaatttt caatattgtt
    45421 ttatagatca ccaatattga aagtcagatg ctatttttta cccacaaaag aacataacaa
    45481 actgattaat aaccaaaatt aacgaaaaac atcctctatt cactcgaaac aaaaatgtga
    45541 caccaatcac agaatacagc ttattgaata cccattatga gttagccatt aacgcgtcca
    45601 cgaggttaat aataattata ttaaatgtta acaaaaataa aacaaacggg aacgcaaaat
    45661 aaatattcgt tttcacagtg gaattaactc atgaagaatg aaaagagaaa aacgggaata
    45721 gaaccgaagg ttttctttcc gccattaata atcgtcggca tactttgttg gcttacagtc
    45781 agagatctgg atgcggcgaa tgtcgttatt aatgctgtat tcagttacgt caccaatgta
    45841 tggggatggg catttgaatg gtatatggtg gtgatgcttt tcggttggtt ctggctggtg
    45901 tttggcccgt atgccaaaaa gcgtttaggt aacgaaccgc cagaatttag caccgccagt
    45961 tggatcttta tgatgttcgc ctcctgtacg tctgctgccg tactgttctg gggatcgatt
    46021 gagatctact actacatctc caccccgccg tttggcttag aaccgaactc gacaggagcg
    46081 aaagagttgg ggctggctta cagcttgttc cactggggac ctctgccgtg ggccacttac
    46141 agcttccttt cagtcgcctt cgcttacttc ttctttgtcc gcaaaatgga agtgattcgc
    46201 cccagctcga cactggtgcc gctggtaggt gaaaaacacg ccaaagggtt gttcggtacc
    46261 atcgtcgaca acttctatct cgtcgccttg atcttcgcga tgggtaccag tctgggcctt
    46321 gccacgccgc tggtgaccga gtgtatgcaa tggttgtttg gcattccgca taccctgcaa
    46381 ctggacgcta tcatcattac ctgctggatt atcctcaacg ccatttgcgt cgcttgcggt
    46441 ctgcaaaaag gggtacgtat cgccagtgac gtgcgtagtt acctgagctt cctgatgctg
    46501 ggttgggtgt tcatcgtcag cggtgccagc ttcatcatga actacttcac cgattcggtg
    46561 gggatgttgc tgatgtatct gccgcgcatg ttgttctata ccgatcccat cgctaaaggc
    46621 ggcttcccgc agggctggac cgtgttctac tgggcatggt gggtgattta cgccatccag
    46681 atgagtatct tcctcgcccg catctcccgt ggtcgtactg tgcgtgaact gtgcttcggc
    46741 atggtgatgg ggctgacagc atccacctgg atcctgtgga ccgtgctcgg tagtaacact
    46801 ctgctgttga tggataaaaa catcatcaac attccaaatc tgatcgaaca gtacggtgtg
    46861 gcgcgcgcca tcatcgaaac ctgggccgct ctgccactca gcaccgccac catgtggggc
    46921 ttcttcatcc tctgctttat tgccaccgtt acgctggtta acgcctgctc ttataccctg
    46981 gcgatgtcta cttgccgcga agtacgcgat ggtgaagaac caccgctgct ggtgcgtatc
    47041 ggttggtcaa ttctggttgg cattatcggt attgttctgc tggcgctcgg cggcctgaaa
    47101 ccgattcaaa ccgccattat cgccggagga tgcccgctgt tcttcgtcaa cattatggtg
    47161 acgctctcct ttattaaaga cgcgaaacag aactggaaag attaattaac ccccaaaata
    47221 tcaagaggtt gaaagatgga ttttaattta aatgatgagc aggaactgtt tgtcgccggt
    47281 atccgcgaac tgatggccag cgaaaactgg gaggcctatt ttgccgagtg cgaccgtgac
    47341 agcgtctacc cggaacgttt tgtcaaagca ctggcggata tgggtatcga cagtctgctg
    47401 atccctgaag agcacggtgg tctggacgcg gggtttgtta ctctcgccgc cgtgtggatg
    47461 gagctgggac gtctgggggc accaacctat gtactgtacc agctgccggg cgggttcaat
    47521 accttcctgc gcgaaggcac acaagagcag atcgacaaga ttatggcttt ccgcggcacc
    47581 ggtaagcaga tgtggaactc agcgattacc gaaccgggcg cgggctccga cgtgggtagc
    47641 ctgaaaacga cttatacccg tagaaatggt aagatttatc ttaatggtag taagtgtttt
    47701 attaccagta gcgcctacac cccgtacatc gtggtgatgg cgcgcgacgg ggcttctccg
    47761 gacaaacctg tctacaccga atggtttgtt gatatgagca aaccgggcat caaagtgacc
    47821 aaacttgaga agctcggtct gcgtatggat agctgctgtg aaatcacctt tgacgatgtg
    47881 gaactggacg agaaagacat gttcggtcgg gaaggtaacg gctttaaccg cgtcaaagaa
    47941 gagttcgacc atgaacgttt cctggtagcc ctgaccaact acggtacggc gatgtgcgcc
    48001 tttgaagatg cggcgcgcta cgccaaccag cgcgtgcagt ttggcgaggc tattggtcgt
    48061 ttccagttga ttcaggaaaa attcgcccac atggcgatca aattaaactc catgaaaaac
    48121 atgctgtatg aagcagcgtg gaaagcagac aacggcacca tcacctctgg cgatgcagcg
    48181 atgtgcaaat acttctgcgc caatgcggca tttgaagtgg tggatagcgc aatgcaggtg
    48241 ctgggcggtg tcgggattgc gggcaaccac cgcatcagcc gcttctggcg tgacctgcgt
    48301 gtagaccgcg tttccggagg atctgacgaa atgcagatcc tgacgctggg tcgtgcggtg
    48361 ctgaagcaat accgctaagt ccgtgcgctg cctgatgcga cgctgacgcg tcttatcagg
    48421 cctacgggag cacgaatgta agccggataa ggcgtttacg ccgcatccgg cagtcatgcg
    48481 ctacataaat ttttcaggag ctaataatgg atcatctacc catgccgaaa ttcggaccgc
    48541 tggccggatt gcgcgttgtc ttctccggta tcgaaatcgc cggaccgttt gccggacaaa
    48601 tgttcgcaga atggggcgcg gaagttattt ggatcgagaa cgtcgcctgg gccgacacca
    48661 ttcgcgttca accgaactac ccgcagctct cccgccgcaa tttgcacgcg ctgtcgttaa
    48721 atattttcaa agatgaaggc cgcgaagcgt ttctgaaatt aatggaaacc accgatatct
    48781 tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat gaagtactgt
    48841 ggcagcacaa cccgaaactg gttatcgctc acctgtccgg ttttggtcag tacggcaccg
    48901 aggagtacac caatcttccg gcctataaca ccatcgccca ggccttcagt ggttacctga
    48961 ttcagaacgg tgatgttgac cagccaatgc ctgccttccc gtataccgcc gattactttt
    49021 ctggcctgac cgccaccacg gcggcgctgg cagcactgca taaagtgcgt gaaaccggta
    49081 aaggcgaaag tatcgacatc gccatgtatg aagtgatgct gcgtatgggc cagtacttca
    49141 tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat gagcaaaggt aaagatccct
    49201 actacgccgg ttgcggtctg tataaatgtg ccgacggcta catcgtgatg gaactggtgg
    49261 gcattaccca aattgaagag tgctttaaag atattggcct cgcacatcta ctcggtacgc
    49321 cggaaatccc ggaaggcact cagcttatcc accgtatcga atgcccttac ggcccactgg
    49381 tagaagaaaa actcgatgcc tggctggcgg cacataccat cgcagaagtt aaagaacgct
    49441 ttgccgaact gaatatcgcc tgtgccaaag tactgaccgt accggaactg gaaagcaatc
    49501 cacagtatgt tgcccgtgaa tcaatcactc agtggcaaac gatggatggt cgcacctgca
    49561 aagggccgaa catcatgccg aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc
    49621 cctcacatgg catggacacg gctgccattt tgaaaaatat cggctacagc gaaaacgaca
    49681 ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc
    49741 ggcttgcctg actagcgccc gcccaataac aaacgaacaa aaatggatag aggtgcaatg
    49801 gatatcattg gcggacaaca tctacgtcaa atgtgggacg atctggcgga cgtttacggt
    49861 cataaaacgg cgctgatttg tgaatccagc ggcggagtcg ttaaccggta tagttatctt
    49921 gagttaaatc aggagattaa ccgcacggca aacctgtttt atacgctggg gattcgcaaa
    49981 ggcaacaagg ttgcactaca tctcgacaac tgcccggaat ttatcttttg ctggttcggg
    50041 ctggcaaaaa ttggcgcgat tatggtgccg attaacgccc gcctgttacg cgaggaaagc
    50101 gagtggatct tgcaaaatag ccaggcgtgc ctgctggtga ccagtgcgca attttatcct
    50161 atgtatcaac agattcagca ggaagatgcc actcaattgc ggcacatttg cctgacagat
    50221 gtggcacttc ccgctgatga tggagtgagt tcatttactc aactgaaaaa tcaacaacct
    50281 gccaccttgt gctatgcacc gccgctatcg actgacgata cggcggaaat tctcttcacc
    50341 tccggcacca cctcccgacc gaaaggtgtg gtgattaccc attacaacct gcgcttcgct
    50401 ggatattact ccgcctggca gtgtgcactg cgtgacgatg acgtctacat gacggtaatg
    50461 cctgcgtttc atatcgattg ccagtgtact gcggcgatgg cggcgttttc tgccggggcc
    50521 acctttgtgc tggtcgagaa atacagcgcc cgcgccttct ggggacaggc gcagaagtac
    50581 cgcgccacca ttaccgaatg tattccgatg atgatccgta cgttgatggt gcagccgcct
    50641 tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt tttatctcaa cttgtcggag
    50701 caggaaaaag acgcattttg tgaacgcttc ggcgttcgct tgctgacgtc ttatgggatg
    50761 acggaaacca ttgtgggcat tatcggcgat cgccctagcg ataaacgacg ctggccgtcg
    50821 attggtcggg cggggttttg ctacgaagcg gagatccgcg acgatcacaa tcgcccgctc
    50881 ccggcaggtg agatcggtga aatctgtatt aaaggcgtac cagggaaaac catcttcaaa
    50941 gagtattttc tcaacccgaa agccactgcg aaagtgctgg aagccgacgg ctggctacat
    51001 accggcgata ccggataccg cgacgaagaa ggcttttttt atttcgtcga tcgccgctgc
    51061 aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg agctggaaaa tatcatcgcc
    51121 acccatccga aaattcagga catcgtggtt gtgggtatta aagattcgat tcgcgatgaa
    51181 gccatcaaag catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga ggaatttttt
    51241 cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct cttatctgga gatcagaaaa
    51301 gatctgccac gtaattgctc ggggaaaata attagaaaga atctgaaata aatgaaacgt
    51361 cagggaacta ccctgccagc taataatcac gccctaaaac aatatgcatt ttttgcaggg
    51421 atgttgtcgt ccctaaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg
    51481 acccgcaatg gatcaattct ggaaattacc cttgatcgcc caaaagcgaa tgctattgat
    51541 gcaaaaacca gctttgaaat gggcgaagta tttctaaatt tccgtgacga tccgcaatta
    51601 cgtgtcgcca ttattaccgg tgccggagag aaattctttt ccgcgggctg ggatttaaaa
    51661 gcggcagcag aaggcgaagc accggatgct gactttggcc cgggtggttt tgcgggatta
    51721 accgaaattt tcaatctcga caaaccagtt atcgcagctg tgaacggcta cgcctttggc
    51781 ggcggtttcg aactggcgct ggcggcagat tttattgttt gtgccgataa cgccagcttc
    51841 gccctgccgg aagccaaact gggcatcgtt cctgacagcg gcggtgtgct gcgtctgccg
    51901 aagatcctgc cgcctaccat cgtcaatgaa atggtgatga ccggcagacg aatgggcgca
    51961 gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat
    52021 aacgcccgcg aactggctca gcagctggtt aacagcgccc cgctggcgat tgcggcgctg
    52081 aaagagatct accgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc
    52141 agcggcgtgt tgaaacacta tccatcggtt ctgcattcgg aagatgccat tgaagggccg
    52201 ctggcgtttg ccgagaagcg cgatccggtg tggaaaggac gttaacaacc gtgagctatt
    52261 acgcctttga ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgccg
    52321 tcttgattgg cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc
    52381 gtggtgacta cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta
    52441 tgcatggcta ctgcgacact gacaccatcg ttggggaaaa cggccatatc gggcatggag
    52501 cgatcctgca tggttgtgtg attggtcgcg atgcattggt cgggatgaac agcgtaatta
    52561 tggatggcgc ggtcattggc gaagagagca ttgttgccgc aatgagcttt gtcaaagcag
    52621 gctttagtgg cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagtg
    52681 ttagtgacga cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg
    52741 ggcgctgtca tgcatcgtta catgaaacgc agccactgag gcaaatggag gaaaatcgcc
    52801 cccgtttgca ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg atgcgacctg
    52861 gttgctggat aagatgctac aacatcgtat ctggcaagca ggacgcccgg tatcaacgag
    52921 ataaaattaa cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag
    52981 ttccgtgagc ggaacggcag gaatacgcgg tgtctttctt acgccgacta atttttcccg
    53041 actgttattg cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt taaccagtcg
    53101 ctgacactgg catccccgcc cttccagctt attagggatc atcttaactt cgcagcttat
    53161 ttctgtgact tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc
    53221 gaaatgaata gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc
    53281 gattaacaag tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca
    53341 aattaaaagt ctggctatga ctcgttttga agcaattaaa caagaccata ttaaaattgt
    53401 ggatatatcg atagtatgca acttcaccgt cgataagtgt gagctaaagc ttatgtcatc
    53461 aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt cctcatcaga
    53521 gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac gacacgctat
    53581 tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc atcggcattc
    53641 agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac tttatattgc
    53701 agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga ggtggtgttg
    53761 atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg gccttcatgc
    53821 accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc cgtgccgtgg
    53881 gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac cacgcgttct
    53941 ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt ggagttgctg
    54001 cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat gacttccccg
    54061 gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt gaacggcagc
    54121 accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc ctgctcagcc
    54181 agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc ggtggctttg
    54241 gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta aacttcgttg
    54301 tttttcaccg caaactgcac gttcatcaga ccgcgtacct gcaattcgaa ggccagtttc
    54361 tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc tggcagcgaa
    54421 catgcggagt caccggagtg cacgcccgcc tgctcgatgt gctccatgat gccgccaatc
    54481 agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc atcaaggaaa
    54541 tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa gtagcgacgc
    54601 aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta agacggacgc
    54661 accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat agcagtaacg
    54721 gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa gcgttcacgg
    54781 tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc agcggcttcc
    54841 agtgcacgcg ccagtttcag cggggtttga ccgccgtact gaacgataac gcctttcggc
    54901 ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa gtagaggcga
    54961 tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat aatggtttcg
    55021 taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa ttcgataccc
    55081 tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc ggtagacgga
    55141 ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt ggcaaactct
    55201 gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc acgcagctta
    55261 cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc aaagcctttg
    55321 cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc cgccactttc
    55381 tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat gttagtcagg
    55441 ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta ccagatacgc
    55501 tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg gtcatccagg
    55561 ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc tttttgcagg
    55621 gattcctgct gtgtgcgacc aatcgccatc acttcgccaa ccgatttcat ctgagtggtc
    55681 agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt agtaaccaca
    55741 tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc gttcatcagt
    55801 tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg gaaaccggtc
    55861 gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat aacaatcagg
    55921 cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc aacgccgatt
    55981 tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc agtcagcgtt
    56041 tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc aaagttttcg
    56101 atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat ctcgtactct
    56161 ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag atccagaccg
    56221 cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc gctaccgccc
    56281 atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac agccagcgct
    56341 tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt cttcatcgct
    56401 acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc accaatcatg
    56461 gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca gttcagcgcc
    56521 gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat aatcttgcgt
    56581 accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg gtcggtcatg
    56641 atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg cagggcttta
    56701 catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat cgggcccgca
    56761 cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt actcctgatt
    56821 acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac aacggcgcgg
    56881 cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc ggtttatcga
    56941 tacgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca cgcaggtttg
    57001 caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc atcaccacgt
    57061 ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc attttgacag
    57121 tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg aataccggaa
    57181 tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg gccgggtcgc
    57241 caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct tccgcagaag
    57301 tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc aggatgttgc
    57361 gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct tttttcgctt
    57421 ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct tctgcggtgg
    57481 tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct ttttctaacg
    57541 ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg cctttctcgc
    57601 gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg ttatggcgct
    57661 tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc ggcaggtcgc
    57721 gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca ttagtgccga
    57781 cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa ggatcagtga
    57841 ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc gccgaacctg
    57901 ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat agcgctgact
    57961 taatcaaaac accctccata gaatattcac tcactttatt tgcatattaa ttcacagtga
    58021 tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc ggcattctgg
    58081 agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt tgtggctcat
    58141 gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg aaaacgcttg
    58201 cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca aatggtcaaa
    58261 atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca gttatagaaa
    58321 atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt ataattaata
    58381 aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat tattgagatc
    58441 aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca aagccgatct
    58501 taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct ccagacgctc
    58561 gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac gcacggtggc
    58621 aaaaccaatg gtaccaggca cacgttcacc ggtatggcct tcacgactgt agaccgcgca
    58681 atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca gtgcggtgcc
    58741 tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat cggtgtagtc
    58801 acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc caacgctaaa
    58861 attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct gtttaccggc
    58921 ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac aaaaagcgag
    58981 atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat catcttttat
    59041 cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca gctcaccggc
    59101 gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct gcacgccttc
    59161 taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc ccgcgatggc
    59221 aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat agaccgtttt
    59281 cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta gaaattaatg
    59341 ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca tgccgataac
    59401 atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg aagcctttta
    59461 cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg tcgatatcaa
    59521 ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa cacggcttga
    59581 gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc attcgcagac
    59641 cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt tttccggtgc
    59701 gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga ttggtgacat
    59761 ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc gcttctggat
    59821 cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca gaaccgccca
    59881 tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt aacagcgcaa
    59941 tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc atcagggcat
    60001 cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca acaaagtcgt
    60061 agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg cgtaccagcg
    60121 gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc agttgcaggg
    60181 cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc agttgcaggt
    60241 cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg ccggggtcag
    60301 tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta acaagaccgg
    60361 cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat cgacacgcag
    60421 ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa tggcttcacc
    60481 accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg gagccgatgc
    60541 gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga tatctttcgc
    60601 atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca gacggttgga
    60661 gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg ccagggcgcg
    60721 taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg gaccgacaat
    60781 tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtatcatcca ccgacagcgt
    60841 ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc acacatcgtc
    60901 tggcgattcg accagataca ttcccccttc ggggttgctg tactgcccca tcgtcccttc
    60961 cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc gactggcgcg
    61021 ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca ccgtcaaatc
    61081 gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga aaatcaggat
    61141 cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct cgcgcaagct
    61201 atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg cgccgtaaat
    61261 ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa aaccacgcgg
    61321 gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc agcacttcaa
    61381 tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta atggagtcgc
    61441 cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta aaaagcatga
    61501 ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag tactgaatca
    61561 ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg gttttgtcgc
    61621 ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca tcgcccaggc
    61681 ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca tcgtcaagtt
    61741 ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac atgcaggttt
    61801 gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca atctgtaggc
    61861 cgcatccggc agggtttatt attgtttttt cgctttggaa ggtaaaaacc cttccagcac
    61921 gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg cgaagtgcca
    61981 gtcgccgacg tagaagtcga tcatatcaac aacgaagccg tgccacaggc ggtcgaacag
    62041 gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct tctgcgtggc
    62101 cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa taccggcaaa
    62161 gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc catagttacg
    62221 cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag caaagttctg
    62281 gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca gccacagcca
    62341 gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt ttcaccgtta
    62401 ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac cttgccgaca
    62461 tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact caacgcgact
    62521 ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt atagtctgca
    62581 acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc ggtcagtttc
    62641 gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc acccactttc
    62701 ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt caacagctcg
    62761 tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc ttcgtaccac
    62821 tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac ttcatcagcg
    62881 gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg atacagcgca
    62941 gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg gtctttgatg
    63001 atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg ctgtaccact
    63061 tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc tttcgcacaa
    63121 cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc ttttgctgga
    63181 tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg acgatagcta
    63241 tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta gtcggttgat
    63301 gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg cggcgaaacg
    63361 gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa gccgtgggtc
    63421 agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag ggaagacatg
    63481 aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg accggcaaat
    63541 tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca tacgtcgaga
    63601 gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt cgcatcgaga
    63661 tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc catcagttcg
    63721 agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat cggtacgccc
    63781 caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga ctcgatacgc
    63841 gcctggcccc agtccgggat ccactgcacg cctttgatct ctttcagtga ctgcgcacgc
    63901 agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat gatcggcgtt
    63961 ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag cagcgcgcct
    64021 ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac gccatccagc
    64081 gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc ggtttccagg
    64141 ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt gtgaacggca
    64201 ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc gaagcccata
    64261 aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc gagaatggtg
    64321 taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc cagaatcgcg
    64381 gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga gattgcgcgg
    64441 ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg gccgttaacg
    64501 ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa agcgacgtca
    64561 atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc agaacggcag
    64621 tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc gatgattttg
    64681 cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata cgggtgcgac
    64741 cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac ctgggtcgcc
    64801 gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc cggcttaccg
    64861 tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc aggcacatac
    64921 ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag aatcttgtta
    64981 accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg cagaatgaag
    65041 gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc agtccaacgc
    65101 gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc tgtttccggc
    65161 aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt cggtttgatt
    65221 acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt taattcatca
    65281 cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt gcgtattttt
    65341 ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc taacaaatgc
    65401 acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt tgccacgccg
    65461 ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt caccggggaa
    65521 acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc taattcatca
    65581 ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc tgccagagcc
    65641 agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac accaccttcg
    65701 caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt ctgtaataac
    65761 aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc aagaaatttt
    65821 acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa cgccgcaaaa
    65881 cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag gtaacgcagt
    65941 ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag ttccagtggt
    66001 tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc ttcctgcaag
    66061 ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat agtcagcaca
    66121 cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt catgtctggc
    66181 tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta accggcgatt
    66241 gagtaccgaa atcagaaaga agtggctttt cattgccatg gcgcaaatca cgggaagaaa
    66301 ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg caagctggta
    66361 gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt atttgcacaa
    66421 atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt gtccatatag
    66481 aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg cgccattcag
    66541 tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac tttcatcaag
    66601 aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc atttaacgaa
    66661 atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa caaagctgca
    66721 cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc aatttgctga
    66781 agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt tgtgatgcgt
    66841 gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt actatctgaa
    66901 ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca agaatatcta
    66961 ctcaatctcc agactccccc acaaagaata tggatattgt gatacacatt gaggtattaa
    67021 acttaagacc actctatttt tgaattaaat tcctatatac tttaaccatt cataaatatc
    67081 tcctcattag acctt
//
LOCUS       DADWTH010000008        65471 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.008, whole genome shotgun sequence.
ACCESSION   DADWTH010000008 DADWTH010000000
VERSION     DADWTH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.008"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(127..606)
                     /gene="folK"
                     /locus_tag="JNY02_000516"
     CDS             complement(127..606)
                     /gene="folK"
                     /locus_tag="JNY02_000516"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBB9432182.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="JNY02_000517"
     CDS             complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="JNY02_000517"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBB9432183.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="JNY02_000518"
     CDS             complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="JNY02_000518"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBB9432184.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3023..3478)
                     /gene="dksA"
                     /locus_tag="JNY02_000519"
     CDS             complement(3023..3478)
                     /gene="dksA"
                     /locus_tag="JNY02_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBB9432185.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="JNY02_000520"
     CDS             complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="JNY02_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBB9432186.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="JNY02_000521"
     CDS             complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="JNY02_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBB9432187.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4979..7408
                     /gene="hrpB"
                     /locus_tag="JNY02_000522"
     CDS             4979..7408
                     /gene="hrpB"
                     /locus_tag="JNY02_000522"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBB9432188.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAQLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7604..10138
                     /gene="mrcB"
                     /locus_tag="JNY02_000523"
     CDS             7604..10138
                     /gene="mrcB"
                     /locus_tag="JNY02_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBB9432189.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10358..12601
                     /gene="fhuA"
                     /locus_tag="JNY02_000524"
     CDS             10358..12601
                     /gene="fhuA"
                     /locus_tag="JNY02_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBB9432190.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12652..13449
                     /gene="fhuC"
                     /locus_tag="JNY02_000525"
     CDS             12652..13449
                     /gene="fhuC"
                     /locus_tag="JNY02_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBB9432191.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13449..14339
                     /gene="fhuD"
                     /locus_tag="JNY02_000526"
     CDS             13449..14339
                     /gene="fhuD"
                     /locus_tag="JNY02_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBB9432192.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14336..16318
                     /gene="fhuB"
                     /locus_tag="JNY02_000527"
     CDS             14336..16318
                     /gene="fhuB"
                     /locus_tag="JNY02_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBB9432193.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLIMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="JNY02_000528"
     CDS             complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="JNY02_000528"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBB9432194.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17858..19279
                     /gene="clcA"
                     /locus_tag="JNY02_000529"
     CDS             17858..19279
                     /gene="clcA"
                     /locus_tag="JNY02_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBB9432195.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19303..19368
                     /locus_tag="JNY02_000530"
     CDS             19303..19368
                     /locus_tag="JNY02_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432196.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19361..19705
                     /gene="erpA"
                     /locus_tag="JNY02_000531"
     CDS             19361..19705
                     /gene="erpA"
                     /locus_tag="JNY02_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBB9432197.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19752..20375)
                     /locus_tag="JNY02_000532"
     CDS             complement(19752..20375)
                     /locus_tag="JNY02_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBB9432198.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREISMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="JNY02_000533"
     CDS             complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="JNY02_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBB9432199.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="JNY02_000534"
     CDS             complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="JNY02_000534"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBB9432200.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21988..23505
                     /gene="dgt"
                     /locus_tag="JNY02_000535"
     CDS             21988..23505
                     /gene="dgt"
                     /locus_tag="JNY02_000535"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBB9432201.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSCSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23635..25059
                     /gene="degP"
                     /locus_tag="JNY02_000536"
     CDS             23635..25059
                     /gene="degP"
                     /locus_tag="JNY02_000536"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBB9432202.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25214..26370
                     /gene="cdaR"
                     /locus_tag="JNY02_000537"
                     /pseudo
     CDS             25214..26370
                     /gene="cdaR"
                     /locus_tag="JNY02_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26459..26845)
                     /locus_tag="JNY02_000538"
     CDS             complement(26459..26845)
                     /locus_tag="JNY02_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBB9432203.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="JNY02_000539"
     CDS             complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="JNY02_000539"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBB9432204.1"
                     /translation="MQQLQNIIETAFERRADITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAIIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="JNY02_000540"
     CDS             complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="JNY02_000540"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBB9432205.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWHIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30748..31542)
                     /gene="map"
                     /locus_tag="JNY02_000541"
     CDS             complement(30748..31542)
                     /gene="map"
                     /locus_tag="JNY02_000541"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBB9432206.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31910..32635
                     /gene="rpsB"
                     /locus_tag="JNY02_000542"
     CDS             31910..32635
                     /gene="rpsB"
                     /locus_tag="JNY02_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBB9432207.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32893..33744
                     /gene="tsf"
                     /locus_tag="JNY02_000543"
     CDS             32893..33744
                     /gene="tsf"
                     /locus_tag="JNY02_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBB9432208.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33891..34616
                     /gene="pyrH"
                     /locus_tag="JNY02_000544"
     CDS             33891..34616
                     /gene="pyrH"
                     /locus_tag="JNY02_000544"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBB9432209.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34908..35465
                     /gene="frr"
                     /locus_tag="JNY02_000545"
     CDS             34908..35465
                     /gene="frr"
                     /locus_tag="JNY02_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBB9432210.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35557..36753
                     /gene="ispC"
                     /locus_tag="JNY02_000546"
     CDS             35557..36753
                     /gene="ispC"
                     /locus_tag="JNY02_000546"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBB9432211.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36942..37700
                     /gene="ispU"
                     /locus_tag="JNY02_000547"
                     /gene_synonym="uppS"
     CDS             36942..37700
                     /gene="ispU"
                     /locus_tag="JNY02_000547"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBB9432212.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37713..38570
                     /gene="cdsA"
                     /locus_tag="JNY02_000548"
     CDS             37713..38570
                     /gene="cdsA"
                     /locus_tag="JNY02_000548"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBB9432213.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38582..39934
                     /gene="rseP"
                     /locus_tag="JNY02_000549"
     CDS             38582..39934
                     /gene="rseP"
                     /locus_tag="JNY02_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBB9432214.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQREAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39964..42396
                     /gene="bamA"
                     /locus_tag="JNY02_000550"
     CDS             39964..42396
                     /gene="bamA"
                     /locus_tag="JNY02_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBB9432215.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42518..43003
                     /gene="skp"
                     /locus_tag="JNY02_000551"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42518..43003
                     /gene="skp"
                     /locus_tag="JNY02_000551"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBB9432216.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43007..44032
                     /gene="lpxD"
                     /locus_tag="JNY02_000552"
     CDS             43007..44032
                     /gene="lpxD"
                     /locus_tag="JNY02_000552"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBB9432217.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44137..44592
                     /gene="fabZ"
                     /locus_tag="JNY02_000553"
     CDS             44137..44592
                     /gene="fabZ"
                     /locus_tag="JNY02_000553"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBB9432218.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44596..45384
                     /gene="lpxA"
                     /locus_tag="JNY02_000554"
     CDS             44596..45384
                     /gene="lpxA"
                     /locus_tag="JNY02_000554"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBB9432219.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45384..46532
                     /gene="lpxB"
                     /locus_tag="JNY02_000555"
     CDS             45384..46532
                     /gene="lpxB"
                     /locus_tag="JNY02_000555"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBB9432220.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46529..47125
                     /gene="rnhB"
                     /locus_tag="JNY02_000556"
     CDS             46529..47125
                     /gene="rnhB"
                     /locus_tag="JNY02_000556"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBB9432221.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47162..50644
                     /gene="dnaE"
                     /locus_tag="JNY02_000557"
     CDS             47162..50644
                     /gene="dnaE"
                     /locus_tag="JNY02_000557"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBB9432222.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50657..51616
                     /gene="accA"
                     /locus_tag="JNY02_000558"
     CDS             50657..51616
                     /gene="accA"
                     /locus_tag="JNY02_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBB9432223.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51715..53856
                     /gene="ldcC"
                     /locus_tag="JNY02_000559"
     CDS             51715..53856
                     /gene="ldcC"
                     /locus_tag="JNY02_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBB9432224.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53913..54302
                     /locus_tag="JNY02_000560"
     CDS             53913..54302
                     /locus_tag="JNY02_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB9432225.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54367..55665
                     /gene="tilS"
                     /locus_tag="JNY02_000561"
     CDS             54367..55665
                     /gene="tilS"
                     /locus_tag="JNY02_000561"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBB9432226.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55714..55974)
                     /gene="rof"
                     /locus_tag="JNY02_000562"
     CDS             complement(55714..55974)
                     /gene="rof"
                     /locus_tag="JNY02_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBB9432227.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55961..56161)
                     /locus_tag="JNY02_000563"
     CDS             complement(55961..56161)
                     /locus_tag="JNY02_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBB9432228.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56327..56872
                     /locus_tag="JNY02_000564"
     CDS             56327..56872
                     /locus_tag="JNY02_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBB9432229.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56869..57291
                     /gene="arfB"
                     /locus_tag="JNY02_000565"
     CDS             56869..57291
                     /gene="arfB"
                     /locus_tag="JNY02_000565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBB9432230.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57305..58015
                     /gene="nlpE"
                     /locus_tag="JNY02_000566"
                     /gene_synonym="cutF"
     CDS             57305..58015
                     /gene="nlpE"
                     /locus_tag="JNY02_000566"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBB9432231.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58170..58994)
                     /locus_tag="JNY02_000567"
     CDS             complement(58170..58994)
                     /locus_tag="JNY02_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBB9432232.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRSIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59047..60765)
                     /gene="proS"
                     /locus_tag="JNY02_000568"
     CDS             complement(59047..60765)
                     /gene="proS"
                     /locus_tag="JNY02_000568"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBB9432233.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="JNY02_000569"
     CDS             complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="JNY02_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBB9432234.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="JNY02_000570"
     CDS             complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="JNY02_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBB9432235.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="JNY02_000571"
     CDS             complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="JNY02_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBB9432236.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62957..63610)
                     /gene="metI"
                     /locus_tag="JNY02_000572"
     CDS             complement(62957..63610)
                     /gene="metI"
                     /locus_tag="JNY02_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBB9432237.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63603..64634)
                     /gene="metN"
                     /locus_tag="JNY02_000573"
     CDS             complement(63603..64634)
                     /gene="metN"
                     /locus_tag="JNY02_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBB9432238.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64822..65397
                     /gene="gmhB"
                     /locus_tag="JNY02_000574"
     CDS             64822..65397
                     /gene="gmhB"
                     /locus_tag="JNY02_000574"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBB9432239.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15464 a        16219 c        18045 g        15743 t
ORIGIN      
        1 gggattgata tttatctgcg ggaatttata ttatgtttct gcaaaaatgt ttaaactaaa
       61 atttttatta aggaaagaga ggatgtatga ttttcagaaa atatttatat tcgtaatata
      121 aataaattac catttgctta atttgtcaaa tgctcttgta tgtaagactt cacgcaacgt
      181 ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc
      241 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc
      301 aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg
      361 accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgggcgaagt
      421 ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt
      481 gcggtaaaac gaagaaacgg caagaatgcg gctttcaggg atatcgccta atgctttcag
      541 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      601 tgtcatgtgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acgacgacgc
      661 ggtgacggtt cttcatccag ctcgttaagc atcccttttt gatctggtgg tgcggaaacc
      721 tggaactcac cccaccattt caccagacgc tgcagttcag cattacgttc aacttcagct
      781 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
      841 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      901 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      961 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
     1021 agcagtgggt accagaacat ggcggcaaac aagaacgccg ggttcacgcg catatcgtta
     1081 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1141 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1201 cacaacagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1261 aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg
     1321 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1381 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1441 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
     1501 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1561 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1621 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
     1681 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1741 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1801 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1861 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac tgtcacctgt
     1921 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1981 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     2041 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     2101 actcgggcac ggcggtcagc atccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2161 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     2221 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2281 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2341 ggtacagcga gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     2401 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     2461 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca
     2521 gcaggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     2581 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     2641 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     2701 gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     2761 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2821 cttctatgcg taccagccag cgaccttgcc gggcgcgacc ctgcaaatag ctgccgagcg
     2881 cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2941 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     3001 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     3061 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3121 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3181 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3241 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3301 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3361 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3421 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3481 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3541 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3601 gaaaagcgta ttcgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3661 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3721 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3781 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3841 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3901 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3961 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttcggagc
     4021 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     4081 ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata aatgcgccgc
     4141 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4201 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     4261 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4321 gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg
     4381 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4441 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cccggcggcg ggaatgtcac
     4501 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4561 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4621 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4681 cgtgagtgtg aaacctggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4741 ctctgcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4801 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcgggtttc
     4861 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4921 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4981 gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc
     5041 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     5101 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     5161 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5221 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5281 aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg gattggtgat
     5341 ccttgatgag tttcatgagc gcagtttgca ggcggatttg gcgttggcgc tgttactcga
     5401 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa
     5461 tgaccgcttg cagcaaatgc tgccggaagc gcccgtcgtc atctcagaag ggcgctcgtt
     5521 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg aagccgttgc
     5581 ggtagccacc gccgaaatgg tgcgtcagga aagtggatca ttactgttat ttttacctgg
     5641 cgtcggagaa attcagcgcg tgcaggaaca actggcttcg cgcatcggca gtgatgtggt
     5701 gctctgcccg ctgtatggcg cgttgtcact gaacgatcag cgaaaagcga tcctcccggc
     5761 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5821 cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgct ttgatccgcg
     5881 cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga cgcagcgtgc
     5941 cgggcgcgcc gggcgtctgg agccgggtat cagcctgcat ttaatcgcca aagaacaagc
     6001 agaacgcgcc gcggcgcaaa gtgagccgga gatcttacaa agcgatcttt ccggtttgct
     6061 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6121 gccaacggtg aatctactgg ccgcgaaacg cctgttacaa atgctggggg cgctggaggg
     6181 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6241 ggcaatgctg gttaacgcaa agagcgatga cgaagctgct accgcggcaa aaattgccgc
     6301 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6361 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgcggcgg
     6421 tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc
     6481 tcatcgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga
     6541 tgccgacgac gcgctaagcc gccacgaatg gttgatcgca cagttattat tgcagggcag
     6601 cgcctcgccg gatgcgcgga ttttactggc gctaccggtc gatattgatg aattagtaca
     6661 acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg cgcaaggtac
     6721 gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg gtgaaagtgc agccgctggc
     6781 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg ataaaggttt
     6841 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6901 aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttgt tggcgacgct
     6961 ggaaacgtgg ctgctgccac atatgaccgg cgtacattca ctacgcggcc tgaaatcact
     7021 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     7081 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7141 agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg ccactaatcc
     7201 gacgatcgcc caggggcgcg tgccgctggt gctgaagttg ctttcccctg cccaaaggcc
     7261 gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg aggtgcaaaa
     7321 ggagatgaaa gggcgttatc ccaaacacgt ctggccgaac gacccggcaa acacggcacc
     7381 gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg
     7441 gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc cctcaaggga
     7501 gaggggaccg atcgagcaca aattttgaga gatatcttct tctgtcttgt aacagaagaa
     7561 cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg
     7621 cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg
     7681 tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat
     7741 gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct
     7801 atggctactg ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta
     7861 tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc ccgcggcagt
     7921 ttatggccga atggtcaacc ttgaaccaga tatgaccatc agcaagaacg aaatggtgaa
     7981 gctgctggag gcaacacagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac
     8041 cgtgcaggcc aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga
     8101 agggcaggtg cgtgcgcgtc tgacctttga tggtgatcat ctggcgacca tcgtcaatat
     8161 ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc
     8221 ctcgccaaac ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg atttgctggt
     8281 ggatactttg ctggcgacag aagaccgtca tttttacgag catgatggaa tcagtctcta
     8341 ctccattggg cgtgcggtgc tggcaaacct gaccgcaggt cgcacggtgc agggtgcgag
     8401 tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgtt cttactggcg
     8461 taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat
     8521 tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg
     8581 cttcccgctg gcaagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca
     8641 gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc
     8701 gaaactggcg ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat
     8761 tgatcaagaa ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc cgcgtggtgg
     8821 ggtgatctct cctcagccag cctttatgca gttggtgcgt caggagttgc aggcaaaact
     8881 gggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc
     8941 ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa
     9001 gttgagcgat cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc
     9061 gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg
     9121 ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc agccgaaaat
     9181 ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca
     9241 ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt
     9301 ggatgcgttg acacgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct
     9361 gcctgcggtt acggagacct ggatcaaact gggcgtaccg aaagatcagt tgcatccggt
     9421 tccggcaatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcattcca
     9481 gaccatcgcc agcggtggta accgtgcacc gctttctgca ctgcgttcgg taatcgcgga
     9541 agatggtaag gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc cggcgcaggc
     9601 ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct
     9661 tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt
     9721 agatacctgg tttgcaggca ttgacggcag cacggtgacc atcacctggg taggccgtga
     9781 taacaaccag ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta
     9841 tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat
     9901 gggcgtggac tacgatggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg
     9961 gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa
    10021 tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga
    10081 gcagaaagac agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca
    10141 tctaagcgtg aaataccgga tggcgagttg ccatccggta aaataacatc ctatctaaga
    10201 tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt
    10261 accgcttgcg aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg
    10321 tttacgttat cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca
    10381 gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc
    10441 tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc
    10501 tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgcggc agtcagctac
    10561 cggcactaaa accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc
    10621 cgaagagatg gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg
    10681 tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac catctgatca ttcgcggttt
    10741 tgcggcagaa ggccaaagcc aaaataacta tctgaatggc ctgaagttgc agggcaactt
    10801 ctataacgat gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc
    10861 ggtttccgtg ctttacggta aaagcagtcc tggcggtctg ttgaatatgg tcagcaagcg
    10921 tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca
    10981 gactggtttt gactttagcg atgcgttgga tgatgacggt gtttactctt atcgcctgac
    11041 cggtcttgcg cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat
    11101 tgcaccggcg ttcacctggc gtccggatga taaaaccaat ttcaccttcc tttcttactt
    11161 ccagaacgag ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc
    11221 gctgccgaac ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta
    11281 ttctcgtaat gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac
    11341 tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta
    11401 cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc
    11461 ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt
    11521 ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct
    11581 gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga
    11641 ctctgtgcca ctgctcaatc tgtacaatcc ggtgaatccc gatttcgact tcaatgccaa
    11701 agatccggca aactccggcc cttaccgcat tctgaataaa cagaaacaaa cgggcgttta
    11761 tgttcaggat caggcgcagt gggataaagt gctggtcacc ctaggcggtc gttatgactg
    11821 ggcagatcaa gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca
    11881 gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag
    11941 ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc
    12001 gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt
    12061 agttactggt gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg
    12121 ttccttcttc tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa
    12181 agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata
    12241 caccaccgat actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc
    12301 gctgtgggct gactatacct tctttgacgg tccgctttca ggtttgacgc tgggcaccgg
    12361 tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta
    12421 tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa
    12481 cgtggcgctg catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac
    12541 ttatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct ttcgtttcta
    12601 atttctcttt tggggcacgg atttccgtgc ccatttcaca agttggctgt tatgcaggaa
    12661 tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg
    12721 cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt
    12781 ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggtcgtca tcagcctccg
    12841 tcagaagggg agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt
    12901 gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat gaccgtgcgt
    12961 gaactggtgg cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggtgcggca
    13021 gatcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg
    13081 ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg
    13141 caggatagcc gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag
    13201 gttgatgtgc tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc
    13261 gtgttgcacg atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcagt
    13321 ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg
    13381 atttatggca tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt
    13441 gtttattgat gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc
    13501 tttctccgtt gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta
    13561 ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg
    13621 gcgtggcgga taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag
    13681 tgattgacgt cggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt
    13741 cgtttatggt ctggtcggca ggatatggcc cttcatcaga aatgctggca cgtattgcgc
    13801 cgggtcgcgg atttaacttc agtgacggca aacatccgtt ggcgatggcg cgtaaatcgc
    13861 tgacggaaat ggcagattta cttaacctgc aaagcgcagc ggaaacgcat ttagcgcaat
    13921 acgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac
    13981 tgctgacgac gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc
    14041 aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg
    14101 gcagtaccgc cgtcagtatc gatcgtctgg cagcgtataa agacgttgat gtgctctgtt
    14161 ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca
    14221 tgccgtttgt ccgtacaggg cgctttcagc gcgtacccgc agtctggttt tatggtgcaa
    14281 cgctttcggc aatgcacttt gtgcgtgttc tggataacgc catcggaggt aaagcgtgag
    14341 taaacgaatt gcgcttttcc cggcgttatt gctggcgctg ttagtgattg tcgctacggc
    14401 gctcacctgg atgaacttct cacaggcgct gccgcgcagt cagtgggcgc aggccgcctg
    14461 gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct
    14521 ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt
    14581 actgcgtaac ccgctggcgg aaccgacgac gttgggcgtt gcaacaggcg cgcaactggg
    14641 gattaccgtc accacgctct gggcgatccc tagcgcgatg gcgagccagt ttgctgcgct
    14701 ggcaggggct tgcgttgttg gcttaattgt ctttggcgtc gcgtggggga aacggctgtc
    14761 gccggtaacg ctgattctcg cggggttggt agtgagcctt tattgcggcg caatcaatca
    14821 gttactggtt atcttccatc atgaccagtt gcaaagcatg tttctgtgga gcactggaac
    14881 gctgacgcaa accgactggg gcggtgttga gcgtttatgg ccgcagttgc tgggcggtgt
    14941 gatgctgacg ttgctgctac ttcgtccgtt aaccctgatg gggcttgatg atggcgtggc
    15001 gcgcaatctc gggctggcct tgtcgcttgc gcgtctggcg gcgctgtcgc tggcgattgt
    15061 catcagtgcg ctgctggtga acgctgtggg gattatcggc tttatcgggt tgttcgcgcc
    15121 gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc tggcgtcgtt
    15181 gattggtgcg ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg
    15241 gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct
    15301 gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gttaacgatc gtgtcgcgac
    15361 tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgttgt tgatggctgt
    15421 ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt
    15481 gctcgaggat ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt
    15541 catgctggcg gtagcgggct gtattattca gcgactgacc ggcaacccga tggcaagccc
    15601 ggaagtgctg gggattagct ccggcgcggc gtttggtgtg gtgttaatgc tgtttctggt
    15661 accgggtaat gcctttggct ggttgttacc cgcaggcagt ctcggggcgg cggtgatgct
    15721 gttgatcatt atgatcgccg ccggacgcgg tggattttcc ccacaccgca tgttactggc
    15781 ggggatggcg ttaagtaccg cgttcaccat gcttttgata atgttgcagg caagtggtga
    15841 cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc
    15901 tcaggtctgg cgcaccggaa ttgtgatggt gattttgctg gcgattactc cgctgtgccg
    15961 ccgctggctg accattttac cgctgggtgg tgataccgcg cgggcggtag ggatggcgct
    16021 gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat
    16081 gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg
    16141 ctttcgacgg acgatgccac atatcgtaat ttcggcgctg gtgggtggtt tactgctggt
    16201 gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatcccgg cggggctgct
    16261 gtcgaccttt atcggtgcgc catattttat ctatttgctg agaaagcaga gccgttaata
    16321 gaatgcctcg cccggtgggc gaggcaaaat tatcacaact tcgcgaacac ccgacgtgca
    16381 gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cagacataaa gcccgcttca
    16441 aacgctgacg gtgccaggta aacaccttca tccagcatca tatggaagaa acgcttaaaa
    16501 cgctcgacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16561 aagataccga acataccacc gacgtggtta acgaccagcg gaattcccgc ttcttctgcc
    16621 gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaatgccc
    16681 ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaatcgg gttaccggaa
    16741 agcgtacccg cctgatagac cggacctgtc ggggccagcg catccattac atcacgacga
    16801 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt ttccgaggca ggttaaatct
    16861 ggcactacgc cgaaataatc ctgtgcgcca gctagcgcta cgcggaaacc ggtcatcact
    16921 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cacgcagacc tggcaggaac
    16981 tctggcagcg gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca
    17041 atctcttgcg ggtattgctc aaacgcggcg cgtacagagg ccagatcgtt ataagtacag
    17101 gttaaggtat gtttggcgaa atctgccgga acgcccggcg agtttggctg gcctaacgtg
    17161 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca
    17221 aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc
    17281 gcctcggtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17341 agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg
    17401 gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat cggcccccag
    17461 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17521 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17581 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt
    17641 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17701 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17761 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17821 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    17881 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    17941 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttatcgggct
    18001 ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct
    18061 ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt
    18121 gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag ggggatcggg
    18181 gatcccggaa attgaagggg cgctggaaga tcaacgtcct gttcgctggt ggcgtgtatt
    18241 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga
    18301 aggaccaacc gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct
    18361 gaaaggtgat gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc
    18421 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18481 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18541 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc
    18601 gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18661 ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18721 taccaaatgg gtactgatgg ggggggcgat tggcggtctg tgtggattgc tggggtttgt
    18781 ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt
    18841 cagcatggga atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt
    18901 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    18961 aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac
    19021 gtttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg
    19081 gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac
    19141 cggtcttggc gcaacactat tagcgcaatt taccggcggg aaacctctat actcggcgat
    19201 tcttgcgcgc acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19261 agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19321 ttagaatttg cccgattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19381 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19441 taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19501 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19561 tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactacac
    19621 cgaaggactg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19681 ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac tatccggcac
    19741 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19801 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19861 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    19921 gttgcgtaga tttctgtacg taaaatcatg gagatttcgc gggccagtac atcacgaatg
    19981 atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc
    20041 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    20101 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    20161 ctggtgacca tggcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    20221 tccagcgcca tgtcgcgaat tgttccgtcg cctactgcgg taaccacgcc cagtaccaga
    20281 acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc
    20341 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga
    20401 aaaggtcagg cactaatcta cctgtgaaag cgcattacag agctgttgtg ctgcgaggat
    20461 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20521 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20581 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20641 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt
    20701 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20761 agcatattgc gctttcaatt gcacgtactg atccagcagg gattgcgcgg cttgttcggc
    20821 cttgtctggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    20881 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    20941 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    21001 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    21061 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    21121 gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agtggcgcga gaaaagacag
    21181 ggcgaccagc gccctgaaca gtgatttagc catgtgccag tttctgcacc agtgactcaa
    21241 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21301 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21361 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21421 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21481 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21541 cagctttaaa gcctgtcgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21601 catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact ttcagcgttg
    21661 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21721 gcagagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21781 cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt
    21841 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21901 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21961 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    22021 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    22081 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    22141 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22201 gaagtccagc aggtgggacg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22261 aaattgctgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag cattgttgag
    22321 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg
    22381 ataaatgact ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca gcctctgact
    22441 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22501 ctgcggcgta agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22561 ctggtgcata cattgatgcg gatgaatctc acttgggcac aggttggcgg tattttaaaa
    22621 tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22681 aagccgggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22741 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc
    22801 tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt
    22861 tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22921 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gctgcagtac ggaagatcag
    22981 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    23041 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    23101 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtattt
    23161 agccatccgg atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23221 gagatttatc gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa
    23281 gaacgggtga aacgtttccc tattgagtcg cgcttattcc acaaactctc gacgcgccat
    23341 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23401 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23461 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23521 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg ggctataaaa
    23581 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23641 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23701 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23761 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23821 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23881 tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat
    23941 ggcggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    24001 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag
    24061 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    24121 gcgctgatcc aaatccagaa cccgaaaaaa ctgaccgcaa ttaagatggc ggattctgat
    24181 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct gggcgagacg
    24241 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga aaactacgaa
    24301 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24361 ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc
    24421 ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24481 tacgggcagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg
    24541 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24601 tcctcggctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24661 ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24721 ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga actgcagcag
    24781 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg cgctgagatg
    24841 agcaacaaag gtaaagatca gggcgtggta gtgaacaacg tgaagacggg cactccggct
    24901 gcgcagatcg gcctgaagaa aggtgatgtg attattggtg cgaaccagca ggcagtgaaa
    24961 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    25021 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    25081 atacgggaag gggttctcct tacaatctgt gaacttcgcc acaactccat acatcttcat
    25141 catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25201 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25261 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25321 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25381 acagggacga gtcgtcgata tcgatgacgc ggtagcacgt catctgcacg gtgtgcggca
    25441 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25501 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25561 ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg aagaactggt
    25621 gatgaacctg attcaggcag aggagaatac tcccgcactt ctgaatgggc gcaacggctg
    25681 gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt
    25741 ggcgtggaca gcgcaatggc ggagttacaa caactgcaaa acgcgctgac tacgcccgag
    25801 cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg
    25861 aactcttttg ggcgctggga tgcagaagat catcgtaagc gagttgaaca actgattacc
    25921 cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac tgggcaacta ttttaccggt
    25981 cctagcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag
    26041 cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac
    26101 agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg
    26161 atggacaata acggcttgct gcgacgaacg ctggcggcgt ggtttcgcca caatgtgcaa
    26221 ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata ccctggagta tcggcttaat
    26281 cgtatatcgg aactgaccgg gcttgatttg ggcaattttg atgacaggtt gctgctgtat
    26341 gtggcgttac aactggatga agagcggtag attatgcgtt aaggttgagg cggtgctggc
    26401 tcattcccct cagcccgaag gggagagggg aatgcccgag ttaaccgtca attcgggatt
    26461 atttacgcgt tagtttttcc agatcggctt caatctcgct gatcttattg gttacgactg
    26521 actccaggtg acgtaagtcg tcgaggatct tacgcttaag atcaacttcg ctgcggtcac
    26581 gctgacagat ttgatcaagc tcatcaatga tataccgtag attcgggctg atttcctgga
    26641 cttctttata accctgaccc acaccatcag cgacgaccgt tttacgctga cgcggatatt
    26701 taaacttaac gctcttggcg aaaaactcgc ctttgtcttt ctggaaatag attttcagaa
    26761 tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat ttcttcagga ttggtaatac
    26821 ccagactttt cagattgtcg tacatagcgt tacctcaaaa tgagtcagta aattgtgctt
    26881 attttagcat ttggcttggc ccgcccggct cgactgtgat cgcagaaagc ctttttcttc
    26941 cagacttcta tcatggcgta aaaatcaaaa aattacctgc tttattgtgg cgatattcca
    27001 tgggatggat tagcacattt atcttcgttt tgcgaattac atgacagtcc gaaacaagat
    27061 gaaaaagtat ttaaaaaagg gggaggaaga atgagtagga cgaattattc tgagccagaa
    27121 agcatacggg cagcatcatg ctgcccgtat atgtactctt aatcgatggt acgcagcagt
    27181 tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aatcgcacag
    27241 tagaggctgt atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc
    27301 ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca
    27361 atgtatacgc ccatagagat gacggaacct tcttcgacaa tcaccccttc aaccacttca
    27421 gagcgtgcgc caatgaagca gttatcttca ataatggtcg ggttagcctg cagcggctcc
    27481 agcacgccgc cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa
    27541 gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgtta
    27601 acgtaagacg gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct
    27661 ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc
    27721 atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata
    27781 cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt
    27841 ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg
    27901 cgggtaacgg tgtctgcatt ggctggcgtg atatcggcac ggcgttcaaa agcggtttca
    27961 ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct
    28021 ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta
    28081 ttaagcgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg
    28141 gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactcgc
    28201 gccagcagtc caggttggtc gagggcgatc agttcgagga acgatttgcg gtcggtatgg
    28261 gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga
    28321 cggggctgcg gtggctgcca gctactttgc gtcagcacct gctccagacc aaaccgaata
    28381 acctcatggc gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc
    28441 atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg
    28501 tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc
    28561 tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca gcggtttgct taaatcatgc
    28621 tgtaataaat ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta
    28681 gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt
    28741 agtgccagtg cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc
    28801 attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc
    28861 cagctattcc acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga
    28921 tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc
    28981 tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca
    29041 accagctgtg tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga
    29101 gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg
    29161 atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc
    29221 tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca
    29281 tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc
    29341 ggcatatacg cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct
    29401 ccggggtgac gcagaatgct caaaaacagt tttcgtgctt gcggaatatt acacagcggt
    29461 tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aataccggtg
    29521 atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata
    29581 aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgagt
    29641 ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc
    29701 atctggttga gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg
    29761 accggttcgt tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg
    29821 aataacagac gattatcgta acggctgacg accagatgca gggcaaagcg aatatgccac
    29881 agtatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaacccg
    29941 accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtga
    30001 atatcgcgca agccgccagg gctacttttg atgtctggtt caaggttgta gctggtgcca
    30061 tggtaacgct gatggcgctg gttctgttct tcaactttcg ctgcgtagaa cttgtcggaa
    30121 ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca
    30181 attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa cccttccaac
    30241 atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc
    30301 gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc
    30361 agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcaacc
    30421 aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg
    30481 tctatgaact cggtacgtgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca
    30541 tcacccagcc aacgctggaa agtatcgata tgggctttta tcccacagac ggttaattca
    30601 tcacggggcc aggcgcatgg attttgcggt tgaccgggca gggtggagag agcggtgttt
    30661 gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat tatcgcatta
    30721 aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt
    30781 gtcatccttg cgtagcgtca aaatttcgca gccgttatca gtcaccacaa tagtatgctc
    30841 atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg
    30901 gatctctttt ttacccgcgt tgaccattgg ctcaatggtg aacgtcatcc ccggtttcag
    30961 cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg
    31021 accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaattt
    31081 ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc gtagcgccag
    31141 gtacaggctt tcttgcgtga tgcggcacag gcgttcgccc atgatggtcg gcttaccgac
    31201 gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt
    31261 aacgatatcg ccatctttca gcagcttagc atcgtccggg ataccgtggc acaccacttc
    31321 attaatagag atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc
    31381 gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc
    31441 cggtttaaca tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg
    31501 cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg
    31561 tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg
    31621 agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata gaaaattcga
    31681 cgtctgatgc tgtacacagc gccaacaatt attggtgtcc acgacgtatt tgtggtataa
    31741 agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac
    31801 tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat
    31861 atgggatacg tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt
    31921 ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa
    31981 cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga
    32041 gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg tttctcgcaa
    32101 aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc
    32161 tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg
    32221 gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg
    32281 tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact
    32341 ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat
    32401 cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacttgggta ttccggtatt
    32461 tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga
    32521 cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg
    32581 ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg
    32641 cttgataact cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg
    32701 ggagcttaca tcagtaagtg accgggatga gcgagcgaag acaacgcatc tgcggcgcga
    32761 aatatgaagg gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca
    32821 tatggccccc tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc
    32881 gaggatttta gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact
    32941 ggcgcaggca tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg
    33001 gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt
    33061 gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt
    33121 aactgccaga ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt
    33181 ctggacgcag ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa
    33241 gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct
    33301 gcgctggaag gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt
    33361 gctgctaaag gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc
    33421 aagccagaat tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag
    33481 gtacagctgg atatcgcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt
    33541 gaaggccgca tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg
    33601 gaaccaagca aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc
    33661 atccgtttcg aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt
    33721 gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt
    33781 tgtgcccatc ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa
    33841 tgtcgctagt attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca
    33901 atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca
    33961 ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac
    34021 tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg
    34081 ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga
    34141 ccgtaatgaa cggcttggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc
    34201 tgatgtccgc tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca
    34261 gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta
    34321 ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag
    34381 caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt
    34441 acgagcaatt gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg
    34501 ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg
    34561 gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc
    34621 cgtgatggat aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta
    34681 taaggcgtta ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca
    34741 aaagtacgtg aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat
    34801 aatccagacg attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt
    34861 gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata
    34921 tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca
    34981 gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt
    35041 acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc
    35101 tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcct
    35161 ccgatctcgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc
    35221 tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc
    35281 gtgttgcagt acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag
    35341 ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg
    35401 atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt
    35461 tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg
    35521 ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc
    35581 tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc
    35641 cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa
    35701 ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg
    35761 ctacagcaac aggggagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg
    35821 gcagcgcttg aggaggttga tcaggtgatg gcagccattg ttggtgctgc tgggctgtta
    35881 cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg
    35941 gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg
    36001 gtcgatagcg aacataacgc tatttttcag agtttaccgc aacctatcca gcataatctg
    36061 ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc
    36121 cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgcccgatca agcttgccgt
    36181 catccgaact ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa
    36241 ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg
    36301 ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg
    36361 gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat
    36421 cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc
    36481 gcaccggatt atgagcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggc
    36541 caggcagcga cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg
    36601 caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat
    36661 atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gagtgaagtc
    36721 gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc
    36781 tatttgttag cgtagggctt caatgatata gtctgcgcca tctgatcgta agtagttggc
    36841 tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt
    36901 cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac
    36961 cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca
    37021 atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa
    37081 aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaagcg ttaacgctgt
    37141 atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac
    37201 tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc
    37261 gtattattgg cgataccagt cgttttaact cgcgtttgca agaacgtatt cgtaaatctg
    37321 aagcgctaac agccggaaat accggtctga cgctgaatat tgcggcgaac tacggtggac
    37381 gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc
    37441 aaccagatca gatagatgaa gagatgttaa accagcatgt ctgtatgcat gaactggccc
    37501 ctgtagattt agtaattagg actgggggag agcatcgcat tagtaacttt ttgctttggc
    37561 aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag
    37621 actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc
    37681 ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt
    37741 ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca
    37801 ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta
    37861 ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc
    37921 tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt
    37981 gggcttcgtt gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg
    38041 cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc
    38101 ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg
    38161 gcgcaatatg gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata
    38221 tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct
    38281 ggcaaggctt tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt
    38341 gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag
    38401 cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca
    38461 gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg
    38521 cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa
    38581 tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac
    38641 cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt
    38701 ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt
    38761 tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt
    38821 cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac gagaggcgat
    38881 tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt
    38941 tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca attcgatagc
    39001 tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc
    39061 tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat
    39121 tacagtagcg ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg
    39181 ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggcc
    39241 gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt
    39301 gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt
    39361 tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg
    39421 gagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg
    39481 ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca
    39541 gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct
    39601 gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg
    39661 gccgatttct atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct
    39721 gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt
    39781 acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc
    39841 cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg
    39901 gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata
    39961 acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac
    40021 ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt
    40081 ggtgcggccc tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc
    40141 agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt
    40201 gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc
    40261 tccggtaaca aatcggtgaa agatgacatg ctaaagcaaa acctcgaggc ttctggtgtg
    40321 cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac
    40381 ttctactaca gcgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg
    40441 cgcaaccgtg ttgacctaaa actggtgttc cgggaaggtg tgtcagctga aatccagcaa
    40501 attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg
    40561 cgtgacgaag tgccgtggtg gaatgtggta ggcgatcgta aataccagaa acagaaactg
    40621 gcgggcgacc ttgaaaccct gcgcagctac tatctggacc gcggttatgc ccgtttcaac
    40681 atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac
    40741 atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg
    40801 cactccgctg aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcact
    40861 aaagtgacca aaatggaaga tgacattaaa aagcttctcg gtcgctatgg ttatgcctat
    40921 ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg
    40981 aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc
    41041 tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gttggggagc
    41101 gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat
    41161 actgataccc aacgtgttcc gggtagcccg gaccaggttg atgtagtcta caaggtgaaa
    41221 gaacgtaaca ctggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc
    41281 ttccaggctg gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac
    41341 gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcacc
    41401 gtagatggcg taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc
    41461 gacctgtccg actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatt
    41521 aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg
    41581 cagcctcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacttct
    41641 gatcaggata acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac
    41701 aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg
    41761 accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg
    41821 ccgatcgatg acgatcacaa atgggttgtt ctgggtcgta cccgctgggg ttatggtgat
    41881 ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc
    41941 gtgcgtggct tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc
    42001 agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg
    42061 tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc
    42121 accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc attcttctgg
    42181 gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac
    42241 tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca
    42301 ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca
    42361 gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt
    42421 ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg
    42481 caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagctgc
    42541 aggtcttggt ttggcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa
    42601 tatgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa
    42661 tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat
    42721 gaaaaaactg cagtccatga aagcgggcag cgatcgcacc aaactggaaa aagacgtgat
    42781 ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg
    42841 ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc
    42901 caacagccag gatatcgatc tggttgttga tgcaaacgct gttgcttaca acagcagcga
    42961 tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg
    43021 actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat
    43081 caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc
    43141 aaaataccgt gagcatttag gcttgtgcca agcgtccgcg gttgtcatga cccaggacga
    43201 tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg
    43261 catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt
    43321 gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga
    43381 gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa
    43441 cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca
    43501 gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta
    43561 tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga
    43621 tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactgttat
    43681 tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga
    43741 caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg
    43801 catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt
    43861 tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat
    43921 tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga
    43981 catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat
    44041 cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat
    44101 tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg
    44161 cagattgaag agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc
    44221 gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag
    44281 ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa
    44341 gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt
    44401 gagctgtact acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc
    44461 gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa
    44521 ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc
    44581 cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa
    44641 gagggcgcgt caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat
    44701 gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa
    44761 attggccgcg ataatgagat ttatcagttt gcctccatcg gcgaagttaa ccaggatttg
    44821 aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc
    44881 gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac
    44941 ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc
    45001 gccaacaacg caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc
    45061 atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg cggctgctcc
    45121 ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc
    45181 ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc
    45241 cgcaatgcgt ataagctaat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa
    45301 attgctgaac tggcggaaac atatccggaa gtgaaagcct ttaccgattt ctttgcacgc
    45361 tcaacgcgcg gtctgattcg ttaatgactg accagcgtcc attaacgatt gccctggtcg
    45421 ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg
    45481 tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag
    45541 cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc
    45601 gtcgcttact gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg
    45661 tttttgttgg tattgatgcg cccgacttca atattactct tgaaggtaac ctcaaaaagc
    45721 agggtatcaa aaccattcat tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg
    45781 ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt
    45841 tttatgacaa atacaatgta ccgtgccgct ttatcggtca taccatggct gatgccatgc
    45901 cgttagatcc agataaaaat ggtgcccgtg atgtgctggg gatcccttac gatgcccact
    45961 gtctggcatt gttgccgggc agccgtggtg cagaagtcga aatgcttagt gccgatttct
    46021 tgaaaactgc ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccgctgg
    46081 tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagacctgt
    46141 cggttcattt gctggatgga atgggccgtg aggcgatggt cgccagcgat gcggcactac
    46201 tggcgtcggg gacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat
    46261 atcgcatgaa gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct
    46321 cgctgccaaa tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg
    46381 agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccacg
    46441 cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg
    46501 cggcacaagc cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct
    46561 ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc
    46621 ggtgatcctt gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga
    46681 aaaacgccgt ctggcgctct atgaagagat caaagagaaa gcgttgagct ggagtctggg
    46741 ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat
    46801 gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg
    46861 ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa ggggatagcc gcgtaccaga
    46921 aatcagtgcc gcgtctatcc tagcgaaagt gacgcgtgac gccgaaatgg cggcgctgga
    46981 tattgttttc ccgcaatatg gttttgccca acataaaggg tatccaaccg cttttcatct
    47041 ggaaaaactg gctgaatatg gcgcgactga acatcatcgt cgcagctttg ggcctgtcaa
    47101 acgcgcactg ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa
    47161 gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg
    47221 cttggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc
    47281 gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc
    47341 agggattaag cctatcgtcg gggcagattt taatgtccag tgcgacctgc tgggtgatga
    47401 gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc tggcgttgct
    47461 gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg
    47521 gcttatcgaa ttaaacgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg
    47581 acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga
    47641 acacttcccg gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag
    47701 ctatctgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa
    47761 cgatgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca
    47821 cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat
    47881 gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc ccagaagccc ttgccaacac
    47941 cgttgagatc gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca
    48001 gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagaaggcct
    48061 ggaagagcgt ctggcatttt tattccctga cgaggaagaa cgtcttaaac gccgcccgga
    48121 atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggtt tcccgggcta
    48181 cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtaggacc
    48241 aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga
    48301 tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc
    48361 tgacttcgac gttgacttct gtatggagaa acgcgatcag gttatcgaac acgtggcgga
    48421 catgtacggt cgtgatgcgg tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc
    48481 ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg accgtatctc
    48541 gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca
    48601 gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa
    48661 actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac
    48721 caaaattacc gattttgcgc cgctttattg cgatgaagag ggcaaacatc cggtcaccca
    48781 gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg
    48841 tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg
    48901 cgagccaccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct
    48961 gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat
    49021 caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc
    49081 tggtccgttg cagtcaggga tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga
    49141 gatctcctat ccggacgtgc agtggcagca tgaaagcctg aaatcggtac tggagccaac
    49201 ctacggcatt atcttgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta
    49261 taccctcggt ggcgcggata tgctgcgccg tgcgatgggt aagaaaaagc cggaagagat
    49321 ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact
    49381 ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca
    49441 ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc
    49501 ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct
    49561 ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct
    49621 ttaccatttc cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg
    49681 ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg
    49741 cgaactgttt gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga
    49801 aaaactgatc atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa
    49861 ctcgctgggt gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca
    49921 ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag
    49981 ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta
    50041 cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt
    50101 aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacagctg cggggctcgt
    50161 tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga
    50221 tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca
    50281 attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag
    50341 cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata
    50401 tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg
    50461 actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca
    50521 gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga
    50581 tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga
    50641 ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct
    50701 ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa
    50761 catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt
    50821 cgccgatctc ggtgcatggc agattgcgca actggcacgc catccgcagc gtccttatac
    50881 cctagattac gttcgcctgg catttgatga atttgacgaa ctggctggcg accgtgcgta
    50941 tgcagacgat aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat
    51001 cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc
    51061 agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat
    51121 gcctatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg
    51181 tggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgccttg gcgtaccggt
    51241 agtttgtacg gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga
    51301 taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc
    51361 cattctgtgg aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc
    51421 tccgcgtctg aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc
    51481 tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc
    51541 cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag
    51601 ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt
    51661 atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac
    51721 atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg
    51781 gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg
    51841 ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac
    51901 agtctcgatt tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc
    51961 atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt
    52021 tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac
    52081 gaatatcttg ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag
    52141 cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg
    52201 gttggctgtc tgttttatga ttttttcggc gggaataccc ttaaggctga tgtctctatt
    52261 tcggtcaccg agcttggttc gttgcttgac cacaccgggc cacacctgga agcggaagag
    52321 tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg
    52381 tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc
    52441 aactgccata aatcactggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg
    52501 aaaccgacgc gtaatgcgtt ggggattctg ggggggatcc cgcgccgtga atttactcgc
    52561 gacagcatcg aagagaaagt cgctgccact acgcaagcac aatggccggt tcatgcggtg
    52621 atcaccaact ccacctatga tggtttgctc tacaacaccg actggatcaa acagacgctg
    52681 gatgtcccgt cgatccactt cgattccgcc tgggtgccgt atactcattt tcatccaatc
    52741 taccagggta aaagtggtat gagcggcgag cgtgttgcgg gaaaagtttt cttcgaaacg
    52801 caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cactgatcca cattaaaggc
    52861 gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt
    52921 tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa
    52981 cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggctg
    53041 cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc
    53101 gaatgctggc ccgttgcgcc tggcgaacaa tggcacggct ttaacgatgc ggatgccgat
    53161 catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc
    53221 aatatgagtg aggaggggat cccggcggcg ctggtagcaa aattcctcga cgaacgtggg
    53281 atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat tggcatcgat
    53341 aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaagcgctc ttacgatctc
    53401 aacctgcgga tcaaaaatat gctgcccgat ctctatgctg aagatcccga tttctaccgc
    53461 aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt
    53521 tccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag
    53581 gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt
    53641 agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga
    53701 gaaatgctga ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta
    53761 gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc
    53821 gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga
    53881 gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata
    53941 ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt
    54001 tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc
    54061 ttaatgggca atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc
    54121 gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgacatc gatgcggcag
    54181 tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc
    54241 aaaaacgctt caccttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt
    54301 aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc
    54361 accatcatga cactcacgct caatagacca cttctcacct cacgccagat tctggtggcc
    54421 tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa
    54481 aatccgggcg tcactctgcg cgctattcat gtgcatcacg gtttaagtgc caatgccgat
    54541 gcctgggtta cgcattgcga aaacgtctgc caacagtggc aggtgccgct ggtggtcgaa
    54601 cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc aggcgcggca ggcgcgttat
    54661 caggcatttg cccgtaccct attgcccggc gaagtgctgg tcaccgcgca acatctcgac
    54721 gatcaatgtg aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg
    54781 gctatggcgg aagtttcgga gtttgctgga acacggctta tccgcccgtt gcttgcccgc
    54841 acgcgggggg aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa
    54901 agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg
    54961 cagcagcgtt ggccgcattt tgcggaagca acggcccgca gcgccacact ttgtgctgaa
    55021 caagagagtc tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag
    55081 gggacgctgc agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatt
    55141 cgccgctggc tggcagggca gaatgcaccg atgccttccc gcgacgcgct ggtgcgcatc
    55201 tggcaggaag tggcgctggc gcgggaagat gcttccccct gtttacgttt gggcgccttt
    55261 gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagcgaa
    55321 accattgtgc tgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaacc
    55381 gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg
    55441 cgtttcaaag cgccaggatt gctgcatatt gtcgggcgca acggcggacg taagctgaag
    55501 aaaatctggc aagagttagg cgtgccgccg tggctacgtg acaccacgcc actgctgttt
    55561 tatggagaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa
    55621 ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa
    55681 gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc
    55741 gatttcaggg tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc
    55801 ggcctcgacg accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg
    55861 caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc
    55921 aagattatcg taatcatcac aattaattgg ttgatacgta tcattcattg acataatcgc
    55981 tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt
    56041 cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc
    56101 ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca
    56161 catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata
    56221 aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg
    56281 cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc
    56341 gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc
    56401 ctctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct
    56461 ggcgtggctg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga
    56521 tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg
    56581 gctgccggat gagcgacgaa ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt
    56641 tacctataat agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca
    56701 gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt
    56761 tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggtgttatct ggttatcgga
    56821 tgataagaat aatctggaag ttaacttaac cgcctggcaa caaccttcat gattgtgatt
    56881 tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc
    56941 gctggcgggc agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg
    57001 gcgcccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc
    57061 agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc
    57121 gaagccgctc tggcccggct ggtggcagtg attaaagatt taacaaaaga acaaaaagcc
    57181 cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa
    57241 aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga
    57301 atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat
    57361 gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg ccgaactgaa
    57421 accgatgccg caaagttggc gcggcgtgct accgtgtgcc gattgcgaag gaatcgaaat
    57481 ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg
    57541 tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt
    57601 attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat
    57661 gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg aaccggcaca
    57721 atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc
    57781 ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct
    57841 ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga
    57901 aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc
    57961 cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaaacctg
    58021 cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg cctacagcat
    58081 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
    58141 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
    58201 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac
    58261 gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc
    58321 gccagtgtaa gtggatgccc ggctttggca aatgcatcag tgacaaattc cgaacaaaac
    58381 cacgactttt tgtctccttc gcacacactg cttaactgcg ctttcgccag gtcgctgacg
    58441 cactgttggc gaaaatcctc ggagaacgga ttcagtgagc acatctggcg agtcaccata
    58501 aagggaataa attcgacaat gctgcgatag ttataaccgc tatctttgat tttattggca
    58561 aacgcggtga tatctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc
    58621 ttatcactat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct
    58681 tctgcaacgt tattatcacc cagaaagatt gcaacgtgac tcacagagga agtgctgaag
    58741 acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg
    58801 agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat
    58861 ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc tggctggctg
    58921 atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc
    58981 tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc aggacccttt
    59041 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
    59101 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
    59161 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
    59221 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
    59281 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
    59341 ctggaacggc gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcaat
    59401 cgccgcagct accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt
    59461 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
    59521 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct
    59581 tggatcgcca gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc
    59641 ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
    59701 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
    59761 cggaccggct ttaaccacag cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc
    59821 aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag
    59881 cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
    59941 ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc
    60001 aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc
    60061 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
    60121 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg
    60181 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
    60241 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
    60301 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
    60361 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
    60421 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
    60481 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg
    60541 ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat
    60601 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
    60661 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
    60721 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
    60781 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca
    60841 gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact
    60901 tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca gacggcatag
    60961 gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg
    61021 cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc
    61081 tctgcggtaa aactcaccgc catctctgca gctggcgcac tttgcgcata gctggcactg
    61141 gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca
    61201 tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct
    61261 ttcagctcta ccagcgacat gccaattggg ttagggcgga aagtagagcg tgttgcgaaa
    61321 acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg ccagccgcct
    61381 tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaatgcttc caggccgcga
    61441 acggcgtcgg cctggttgta ggaagcaatg agatgcagtt cgccgttggc gctttttacc
    61501 agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt
    61561 tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg atacatacag
    61621 cctgacgata gcagcccggc gtaccgctgg tgacttcgca gctatgcagt aatacagcat
    61681 tggctttcat tttagaggca ttgatttgca tctgcttacg tgcggttgga atgctcggcg
    61741 gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg
    61801 gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt
    61861 tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg
    61921 atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac
    61981 gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa
    62041 tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa
    62101 attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcat
    62161 cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg
    62221 ttacgatcag gtttacgtat ggggagtctt tatcttcgac aaagataccg tctttcgccg
    62281 gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt
    62341 cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga ttttttgggt
    62401 tctcaacaac atcaagaact gtcggcagca ggccaacgcc atctttcagt ttgatcaagc
    62461 ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt ggcacggcaa
    62521 cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa ccagcaatcg
    62581 gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat
    62641 caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt
    62701 ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg
    62761 caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg ccgactttaa
    62821 tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg
    62881 ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt
    62941 tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact
    63001 gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttgt
    63061 agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac
    63121 ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg
    63181 gcaacgcttc cggtaacagc actttacgga cgatctgcat tggcgtggca cccattgcgc
    63241 gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc atacgggcaa
    63301 taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca atcgatgtac
    63361 cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac
    63421 ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca ataatttgcc
    63481 ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaac ccaaaaaatc
    63541 cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg
    63601 gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg
    63661 gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa tttaacgcca
    63721 ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt
    63781 tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg
    63841 cagtcagtaa atggctccgt ttgcagacgt tcctggtaat cttccgggat atccagatgc
    63901 agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt
    63961 accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc acaaatacgc
    64021 ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg gcggttaatg
    64081 tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca
    64141 tcacacagca atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc
    64201 tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac
    64261 agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag ctccagcggc
    64321 agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata
    64381 ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcaa ttcctggcca
    64441 tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta
    64501 ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg
    64561 ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc
    64621 gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtagca tctcgaatgc
    64681 ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat
    64741 caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcccatgc
    64801 cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat
    64861 ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga atttatcgac
    64921 ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc
    64981 aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac gctgaccgag
    65041 tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta ttattgcccg
    65101 catcatccgc aggggagtgt tgaagagttt cgccaggtct gcgattgccg caaaccacat
    65161 ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg
    65221 gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg
    65281 ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga ttgggtgtta
    65341 aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc gcaatgatta
    65401 aaagatgaac ggttgaaata aaaatgcatt tttccgcttg tcttcctgag ccgactccct
    65461 ataatgcgcc t
//
LOCUS       DADWTH010000009        65142 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.009, whole genome shotgun sequence.
ACCESSION   DADWTH010000009 DADWTH010000000
VERSION     DADWTH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.009"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JNY02_000575"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JNY02_000575"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBB9432240.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JNY02_000576"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JNY02_000576"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBB9432241.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JNY02_000577"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JNY02_000577"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBB9432242.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JNY02_000578"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JNY02_000578"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBB9432243.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPTRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JNY02_000579"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JNY02_000579"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBB9432244.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="JNY02_000580"
     CDS             7826..9559
                     /locus_tag="JNY02_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBB9432245.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JNY02_000581"
     CDS             9708..10559
                     /locus_tag="JNY02_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9432246.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JNY02_000582"
     CDS             complement(10546..10887)
                     /locus_tag="JNY02_000582"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBB9432247.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JNY02_000583"
     CDS             complement(10889..11767)
                     /locus_tag="JNY02_000583"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBB9432248.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCSWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JNY02_000584"
     CDS             complement(11733..14030)
                     /locus_tag="JNY02_000584"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBB9432249.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQGRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGHDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JNY02_000585"
     CDS             complement(14081..14401)
                     /locus_tag="JNY02_000585"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBB9432250.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="JNY02_000586"
     CDS             complement(14416..15495)
                     /locus_tag="JNY02_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBB9432251.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="JNY02_000587"
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="JNY02_000587"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBB9432252.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="JNY02_000588"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="JNY02_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20082
                     /gene="gldA"
                     /locus_tag="JNY02_000589"
                     /pseudo
     CDS             18990..20082
                     /gene="gldA"
                     /locus_tag="JNY02_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20357..20974
                     /locus_tag="JNY02_000590"
     CDS             20357..20974
                     /locus_tag="JNY02_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBB9432253.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPDGDVPQECGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21001..21906)
                     /gene="yijE"
                     /locus_tag="JNY02_000591"
     CDS             complement(21001..21906)
                     /gene="yijE"
                     /locus_tag="JNY02_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBB9432254.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22000..24180)
                     /gene="katG"
                     /locus_tag="JNY02_000592"
     CDS             complement(22000..24180)
                     /gene="katG"
                     /locus_tag="JNY02_000592"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBB9432255.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24509..25399)
                     /gene="metF"
                     /locus_tag="JNY02_000593"
     CDS             complement(24509..25399)
                     /gene="metF"
                     /locus_tag="JNY02_000593"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBB9432256.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25748..28180)
                     /gene="metL"
                     /locus_tag="JNY02_000594"
     CDS             complement(25748..28180)
                     /gene="metL"
                     /locus_tag="JNY02_000594"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBB9432257.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28183..29343)
                     /gene="metB"
                     /locus_tag="JNY02_000595"
     CDS             complement(28183..29343)
                     /gene="metB"
                     /locus_tag="JNY02_000595"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBB9432258.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29620..29937
                     /gene="metJ"
                     /locus_tag="JNY02_000596"
     CDS             29620..29937
                     /gene="metJ"
                     /locus_tag="JNY02_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBB9432259.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30121..30729
                     /locus_tag="JNY02_000597"
     CDS             30121..30729
                     /locus_tag="JNY02_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBB9432260.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="JNY02_000598"
     CDS             complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="JNY02_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBB9432261.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31205..33403
                     /gene="priA"
                     /locus_tag="JNY02_000599"
     CDS             31205..33403
                     /gene="priA"
                     /locus_tag="JNY02_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBB9432262.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33559..34584
                     /gene="cytR"
                     /locus_tag="JNY02_000600"
     CDS             33559..34584
                     /gene="cytR"
                     /locus_tag="JNY02_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBB9432263.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34676..35635
                     /gene="ftsN"
                     /locus_tag="JNY02_000601"
     CDS             34676..35635
                     /gene="ftsN"
                     /locus_tag="JNY02_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBB9432264.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35728..36258
                     /gene="hslV"
                     /locus_tag="JNY02_000602"
     CDS             35728..36258
                     /gene="hslV"
                     /locus_tag="JNY02_000602"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBB9432265.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36268..37599
                     /gene="hslU"
                     /locus_tag="JNY02_000603"
     CDS             36268..37599
                     /gene="hslU"
                     /locus_tag="JNY02_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBB9432266.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37666..38592
                     /gene="menA"
                     /locus_tag="JNY02_000604"
     CDS             37666..38592
                     /gene="menA"
                     /locus_tag="JNY02_000604"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBB9432267.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38685..39170
                     /gene="rraA"
                     /locus_tag="JNY02_000605"
     CDS             38685..39170
                     /gene="rraA"
                     /locus_tag="JNY02_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBB9432268.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="JNY02_000606"
     CDS             complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="JNY02_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBB9432269.1"
                     /translation="MTMSLEVFDKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39926..40771
                     /gene="glpF"
                     /locus_tag="JNY02_000607"
                     /pseudo
     CDS             39926..40771
                     /gene="glpF"
                     /locus_tag="JNY02_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40811..42301
                     /gene="glpK"
                     /locus_tag="JNY02_000608"
     CDS             40811..42301
                     /gene="glpK"
                     /locus_tag="JNY02_000608"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBB9432270.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42531..43541
                     /gene="glpX"
                     /locus_tag="JNY02_000609"
     CDS             42531..43541
                     /gene="glpX"
                     /locus_tag="JNY02_000609"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBB9432271.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43638..44384
                     /gene="fpr"
                     /locus_tag="JNY02_000610"
     CDS             43638..44384
                     /gene="fpr"
                     /locus_tag="JNY02_000610"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBB9432272.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="JNY02_000611"
     CDS             complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="JNY02_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBB9432273.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44844..45143)
                     /locus_tag="JNY02_000612"
     CDS             complement(44844..45143)
                     /locus_tag="JNY02_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBB9432274.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAAFSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45355..45795)
                     /locus_tag="JNY02_000613"
                     /pseudo
     CDS             complement(45355..45795)
                     /locus_tag="JNY02_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45896..46495
                     /locus_tag="JNY02_000614"
     CDS             45896..46495
                     /locus_tag="JNY02_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBB9432275.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46603..47370
                     /gene="tpiA"
                     /locus_tag="JNY02_000615"
     CDS             46603..47370
                     /gene="tpiA"
                     /locus_tag="JNY02_000615"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBB9432276.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="JNY02_000616"
     CDS             complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="JNY02_000616"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBB9432277.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48287..49276)
                     /gene="sbp"
                     /locus_tag="JNY02_000617"
     CDS             complement(48287..49276)
                     /gene="sbp"
                     /locus_tag="JNY02_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBB9432278.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="JNY02_000618"
     CDS             complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="JNY02_000618"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBB9432279.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="JNY02_000619"
     CDS             complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="JNY02_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBB9432280.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="JNY02_000620"
     CDS             complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="JNY02_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBB9432281.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52439..53137
                     /gene="cpxR"
                     /locus_tag="JNY02_000621"
     CDS             52439..53137
                     /gene="cpxR"
                     /locus_tag="JNY02_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBB9432282.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53134..54507
                     /gene="cpxA"
                     /locus_tag="JNY02_000622"
     CDS             53134..54507
                     /gene="cpxA"
                     /locus_tag="JNY02_000622"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBB9432283.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="JNY02_000623"
     CDS             complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="JNY02_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBB9432284.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="JNY02_000624"
     CDS             complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="JNY02_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBB9432285.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="JNY02_000625"
     CDS             complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="JNY02_000625"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBB9432286.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57584..58618
                     /gene="rhaT"
                     /locus_tag="JNY02_000626"
                     /pseudo
     CDS             57584..58618
                     /gene="rhaT"
                     /locus_tag="JNY02_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58587..59480)
                     /gene="rhaR"
                     /locus_tag="JNY02_000627"
     CDS             complement(58587..59480)
                     /gene="rhaR"
                     /locus_tag="JNY02_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBB9432287.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRISIRRKINSPCRCRRC"
     gene            complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="JNY02_000628"
                     /pseudo
     CDS             complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="JNY02_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            60660..62129
                     /gene="rhaB"
                     /locus_tag="JNY02_000629"
     CDS             60660..62129
                     /gene="rhaB"
                     /locus_tag="JNY02_000629"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBB9432288.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62126..63385
                     /gene="rhaA"
                     /locus_tag="JNY02_000630"
     CDS             62126..63385
                     /gene="rhaA"
                     /locus_tag="JNY02_000630"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBB9432289.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63627..64451
                     /gene="rhaD"
                     /locus_tag="JNY02_000631"
     CDS             63627..64451
                     /gene="rhaD"
                     /locus_tag="JNY02_000631"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBB9432290.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            64461..64775
                     /gene="rhaM"
                     /locus_tag="JNY02_000632"
     CDS             64461..64775
                     /gene="rhaM"
                     /locus_tag="JNY02_000632"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBB9432291.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            65076..>65142
                     /locus_tag="JNY02_000633"
     CDS             65076..>65142
                     /locus_tag="JNY02_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB9432292.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT        15882 a        17551 c        16664 g        15045 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gccaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccaacacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcacca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctaggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttcct ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atagcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgcgcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac attttgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgagcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc agcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cgtgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggccctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccaatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggagtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt cgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctggctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gccgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaag agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcctag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgtgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcagtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctctaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    19801 gtggcatttg gtacgctgac gcagctggta ctggaaaacg cgccggtgga ggaaatcgaa
    19861 accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    19921 aaagaagatg tcccggcgaa aatgcgcatt gttgcaaaag catcttgtgc agaaggtgaa
    19981 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc actgctggta
    20041 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20101 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20161 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20221 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20281 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20341 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20401 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    20461 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20521 ggcgtatatg cagcttacct atcctgacgg tgatgttccg caagaatgcg gtgtatgttc
    20581 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20641 agatatggcg aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag
    20701 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20761 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20821 agataacggg ctggcgcata ttggggtggc gtcagatggc ttcgcccgcg acggtacgcc
    20881 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20941 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    21001 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21061 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21121 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21181 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21241 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21301 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21361 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21421 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21481 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21541 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21601 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21661 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21721 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21781 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21841 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21901 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21961 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    22021 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22081 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22141 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22201 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22261 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22321 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22381 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22441 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22501 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22561 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22621 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22681 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg acaccaccgc gtttgtcgcc
    22741 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22801 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22861 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22921 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22981 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    23041 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23101 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23161 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23221 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23281 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23341 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23401 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23461 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23521 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23581 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23641 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23701 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23761 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23821 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23881 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23941 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    24001 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24061 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24121 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24181 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24241 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24301 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24361 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24421 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24481 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24541 aaatcgcgta actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24601 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24661 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24721 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24781 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24841 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24901 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24961 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    25021 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacaaata ccgttattcc
    25081 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25141 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25201 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25261 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25321 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25381 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25441 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25501 ccgaaaaatg accaatgatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25561 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25621 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25681 tcaatatatt tcattagaaa ccatagcccc gaaaacatga gtaccgggca ttattaaatt
    25741 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25801 cgtcgcgccc agcgccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25861 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25921 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25981 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    26041 tttcaaagaa atggtcgatg ctgccacctt cgcaatgagc aggcaccagc gattccacac
    26101 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26161 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26221 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26281 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26341 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26401 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26461 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26521 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26581 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26641 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26701 gcacaaattc aaagccagta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26761 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26821 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26881 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26941 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    27001 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27061 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27121 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27181 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27241 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27301 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27361 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27421 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27481 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27541 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27601 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27661 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27721 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27781 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27841 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27901 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27961 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    28021 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28081 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28141 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28201 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28261 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28321 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28381 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28441 actcaacatt gcgccaaagc ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28501 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28561 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28621 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28681 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28741 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28801 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28861 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28921 taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac
    28981 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    29041 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29101 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29161 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29221 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29281 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29341 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29401 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29461 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29521 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag taccaacata aattgtgggg
    29581 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29641 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29701 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29761 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29821 gggcaacctt tgccggatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29881 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29941 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    30001 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30061 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30121 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag
    30181 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30241 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30301 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30361 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30421 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30481 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30541 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30601 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30661 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30721 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30781 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30841 accggtagca acatcacgct gtttgccggt gaagaacggg tggcacttgc tgcacacgtc
    30901 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30961 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    31021 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31081 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31141 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31201 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31261 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31321 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31381 gctaaaagcg gtagtcgaag tgctggatgg tgagccggtg tttactcact ccgtctggcg
    31441 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31501 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31561 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31621 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31681 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31741 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31801 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31861 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31921 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31981 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    32041 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32101 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32161 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32221 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32281 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg
    32341 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32401 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32461 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32521 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32581 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32641 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32701 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32761 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32821 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32881 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32941 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    33001 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33061 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33121 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33181 gatcctctcc agtccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33241 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33301 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33361 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33421 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33481 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33541 accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33601 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33661 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33721 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33781 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33841 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33901 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33961 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    34021 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34081 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34141 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcgga
    34201 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34261 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34321 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34381 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34441 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34501 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34561 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34621 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34681 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34741 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34801 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
    34861 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34921 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34981 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    35041 tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35101 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35161 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35221 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35281 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35341 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa
    35401 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35461 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35521 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35581 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35641 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35701 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35761 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35821 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35881 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35941 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    36001 cttgaagcac tgctggcagt cgctgatgaa actgcatcgc ttatcatcac cggtaacggt
    36061 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36121 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36181 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36241 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36301 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36361 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36421 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36481 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36541 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36601 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
    36661 ctcgacgtgc tgatcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36721 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36781 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36841 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36901 aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36961 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    37021 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37081 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37141 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37201 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37261 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37321 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37381 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37441 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37501 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37561 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37621 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37681 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37741 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37801 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37861 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37921 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37981 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    38041 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38101 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38161 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38221 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38281 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38341 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38401 ttttcgctgc atagcctgtg gggctggctg tttctgctgg cggcaccatt actggtgaag
    38461 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38521 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38581 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38641 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    38701 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38761 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38821 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    38881 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38941 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    39001 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39061 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39121 ccgacaatac cgggattatt ctttcagaag atccgctaga tattgaatga taagaaaggc
    39181 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39241 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39301 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39361 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39421 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttatcaaac
    39481 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39541 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39601 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39661 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39721 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39781 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39841 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39901 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39961 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    40021 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40081 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40141 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40201 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40261 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40321 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40381 gatggtgatt accgctattc tgatggggct tatcctggcg ttaacggacg atggcaacgg
    40441 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40501 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40561 ctttgcctag ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40621 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40681 caaactgatt ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac
    40741 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40801 aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga
    40861 tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc
    40921 aggttgggta gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga
    40981 agtgctggcg aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca
    41041 gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt
    41101 ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga
    41161 ttatatcagc agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa
    41221 gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt
    41281 tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga
    41341 ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa
    41401 aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga
    41461 agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat
    41521 cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa
    41581 gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc
    41641 agaaaatggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtga actatgcgtt
    41701 ggaaggtgcg gtgtttatgg caggcacatc cattcagtgg ctgcgcgatg agatgaagtt
    41761 gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt
    41821 gtatgtggtt ccggcattta ccgggctgag tgcgccgtac tgggacccgt atgcgcgcgg
    41881 ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga
    41941 gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg
    42001 tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca
    42061 gtccgatatt ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg
    42121 tgcggcctat ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga
    42181 gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg
    42241 ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta
    42301 atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct
    42361 tatcaggcct acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc
    42421 cggcatcacg cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac
    42481 ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag
    42541 aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt
    42601 taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc
    42661 tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac
    42721 cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg
    42781 ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc
    42841 tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga
    42901 ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc
    42961 gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta
    43021 aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta
    43081 ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg
    43141 tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat
    43201 tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag
    43261 agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg
    43321 gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg
    43381 gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg
    43441 aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc
    43501 tggatcgcaa agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc
    43561 cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat
    43621 caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact
    43681 ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc
    43741 aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg
    43801 ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat
    43861 taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag
    43921 ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg
    43981 gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca
    44041 aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga
    44101 tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg
    44161 aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg
    44221 aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc
    44281 cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt
    44341 tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat
    44401 aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat
    44461 caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg
    44521 cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg
    44581 ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg
    44641 aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc
    44701 aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac
    44761 cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt
    44821 taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc
    44881 atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc
    44941 ttaatcttat aggccacgct ggccccttca tctgcagaga atgcagcggc ggcaagctct
    45001 ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta
    45061 ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg
    45121 catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg
    45181 acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca
    45241 ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg
    45301 catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggtcaatt
    45361 acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc
    45421 gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg
    45481 gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgctcagt tacccgccag
    45541 cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa
    45601 gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt
    45661 ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca
    45721 caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca
    45781 ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga
    45841 caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa
    45901 gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca
    45961 tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt
    46021 cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact
    46081 gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag
    46141 taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa
    46201 aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa
    46261 agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca
    46321 aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga
    46381 gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc
    46441 tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa
    46501 agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg
    46561 cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg
    46621 atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg
    46681 cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac
    46741 atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg
    46801 gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc
    46861 ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac
    46921 gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc
    46981 atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag
    47041 atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttat
    47101 gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt
    47161 cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc
    47221 attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac
    47281 atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt
    47341 aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc
    47401 acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc
    47461 ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga
    47521 attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg
    47581 cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg
    47641 acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat
    47701 gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat
    47761 atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc
    47821 gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa
    47881 gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt
    47941 tgacatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt
    48001 gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc
    48061 ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat
    48121 accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat
    48181 ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa
    48241 acccggtcgc gagaaaccac cgggtttgat aattatccta gggaaatcag cgtttgctga
    48301 tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga
    48361 actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta
    48421 cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt
    48481 agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat
    48541 cgaccaccga caccgttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt
    48601 tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat
    48661 cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt
    48721 cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt
    48781 gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg
    48841 gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct
    48901 tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg
    48961 gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat
    49021 aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt
    49081 tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt
    49141 gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt
    49201 taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta
    49261 cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca
    49321 gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt
    49381 gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttgtcca
    49441 tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt caataaattg
    49501 cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt
    49561 ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc
    49621 agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga
    49681 accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag cagcagatcg
    49741 atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg
    49801 cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa
    49861 tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta
    49921 cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc
    49981 ggaactacaa cgaattcaca gccaccggca atggccgcag ccagggtcag atcgccacaa
    50041 taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga
    50101 cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg
    50161 cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc catttcggtc
    50221 agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca
    50281 cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa
    50341 cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct gtaacggtct
    50401 agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc cattacttcc
    50461 agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca
    50521 tcaccgccgc ttgtcaacac acctattttc ttaatcatga ctacctctga actttggaat
    50581 gcaaaatgaa atctgttgcc ggaaatcttc ttgcacatcg aagtgatcca acgaatgtgc
    50641 aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt
    50701 tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta
    50761 ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa
    50821 cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga
    50881 gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga
    50941 agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt
    51001 tcttcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac
    51061 gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag
    51121 ccgtaccaag acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg
    51181 taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga
    51241 aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct
    51301 ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa caggaatagt
    51361 gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga
    51421 ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag taaattcgtc
    51481 aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg
    51541 gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc cgtcgcagca
    51601 atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac
    51661 tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg
    51721 ataaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac
    51781 agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt
    51841 tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc
    51901 gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc
    51961 tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca
    52021 aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc
    52081 tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa
    52141 cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc
    52201 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag
    52261 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    52321 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    52381 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    52441 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    52501 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    52561 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    52621 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    52681 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    52741 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    52801 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    52861 gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga
    52921 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    52981 aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat
    53041 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    53101 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    53161 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    53221 ctcgattcac gccagatgac caagcttctg gatagcgaac agcgtcaggg gctgatgatt
    53281 gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg
    53341 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    53401 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    53461 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    53521 ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    53581 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt
    53641 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    53701 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    53761 gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc
    53821 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    53881 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt
    53941 gaaagcaagg aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    54001 gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    54061 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc
    54121 aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    54181 ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt
    54241 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    54301 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    54361 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    54421 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    54481 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    54541 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    54601 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    54661 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    54721 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    54781 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    54841 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    54901 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    54961 tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt
    55021 tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    55081 cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    55141 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    55201 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    55261 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    55321 accatattta ttttatgtca gccgtgctgt tttcgcggta agtatccagg caggaactca
    55381 atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    55441 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    55501 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    55561 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
    55621 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    55681 atttatcggc gataatcaac ggaataccgg tcacgataat tattgccaca cccaacagga
    55741 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
    55801 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    55861 gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    55921 caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    55981 acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc gtactgctgc atgatggaag
    56041 cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    56101 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    56161 cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg
    56221 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg
    56281 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    56341 tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa
    56401 tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtaa
    56461 ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa
    56521 agtgatcaaa accacatcaa ttgaaactct gttttatttt tataggatca ttgtttttat
    56581 tttggaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata
    56641 tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt
    56701 gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc cggacggcgg
    56761 ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg
    56821 gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc
    56881 gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc
    56941 gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct
    57001 ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta
    57061 gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt
    57121 ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg
    57181 ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta
    57241 tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata
    57301 ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggtttatta
    57361 tagttaatta aatgatattg aaaatgatta tcaatgccgc acttttcgta agggtatggt
    57421 tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    57481 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    57541 tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    57601 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    57661 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    57721 gattattctg ccgtgggcca tcagcgccct gttactaccg gatttctggg cgtattacag
    57781 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    57841 taatatcaac tatggcctga ccatgcgtta cctcggcatg tcgatgggaa ttggcatcgc
    57901 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    57961 tgtgctgatt aacaccgaag gcggacgcat gacgttactc ggcgttctgg tggcgctgat
    58021 tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc
    58081 cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    58141 cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcacttgg
    58201 cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat
    58261 cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    58321 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg cactgggcgg
    58381 gttgatgtgg tatctgcaat tctttttcta tgcctagggc cacgcccgca ttccggcgca
    58441 gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt
    58501 cgggctggtg ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg tgttgagcct
    58561 cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc
    58621 tttctgcgaa ttgagatgcg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    58681 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    58741 cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg
    58801 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    58861 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
    58921 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    58981 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
    59041 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    59101 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    59161 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    59221 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    59281 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    59341 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    59401 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    59461 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    59521 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    59581 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    59641 ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcagg ctttcatcag
    59701 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    59761 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    59821 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    59881 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    59941 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    60001 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    60061 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    60121 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
    60181 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    60241 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    60301 cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactatg
    60361 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    60421 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    60481 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
    60541 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    60601 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    60661 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
    60721 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    60781 ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc
    60841 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    60901 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    60961 cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac
    61021 gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc
    61081 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    61141 cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca
    61201 ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    61261 gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    61321 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    61381 cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt
    61441 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    61501 tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    61561 ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg
    61621 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
    61681 atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc
    61741 cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg
    61801 atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg
    61861 gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg
    61921 tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag ttaattacgc
    61981 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    62041 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta
    62101 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    62161 gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg
    62221 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    62281 aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc
    62341 cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    62401 taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc
    62461 agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    62521 cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    62581 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    62641 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    62701 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    62761 cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    62821 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    62881 ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa
    62941 gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc gtccggttcg
    63001 ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat
    63061 tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat
    63121 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    63181 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    63241 tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    63301 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    63361 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacgagcgc cttatccggc
    63421 ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc
    63481 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    63541 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    63601 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    63661 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca
    63721 acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac
    63781 aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    63841 tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg
    63901 tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag
    63961 ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
    64021 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    64081 atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat
    64141 gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    64201 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    64261 acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc gacaccgcag
    64321 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    64381 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc
    64441 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
    64501 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgttga
    64561 aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    64621 ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact gatgtttgcc
    64681 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac agcccggtga
    64741 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg gagatgcctc
    64801 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    64861 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    64921 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    64981 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    65041 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    65101 ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DADWTH010000010        64479 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.010, whole genome shotgun sequence.
ACCESSION   DADWTH010000010 DADWTH010000000
VERSION     DADWTH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.010"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            334..1050
                     /locus_tag="JNY02_000634"
     CDS             334..1050
                     /locus_tag="JNY02_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9432293.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQNKAAAIAEF
                     KELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAEEDLRFDSKEEQLPEER
                     EQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLYTWMVSKCRYRGNDKYE
                     IWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGKCGKTYRLRIPSKGTAE
                     EASMTAQQHEREAKVSYLVH"
     gene            2101..2853
                     /locus_tag="JNY02_000635"
     CDS             2101..2853
                     /locus_tag="JNY02_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432294.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIYTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            3613..4008
                     /locus_tag="JNY02_000636"
     CDS             3613..4008
                     /locus_tag="JNY02_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB9432295.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHKGKFKVTLTYQKACYIYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            4026..4289
                     /locus_tag="JNY02_000637"
     CDS             4026..4289
                     /locus_tag="JNY02_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432296.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            4312..4854
                     /locus_tag="JNY02_000638"
     CDS             4312..4854
                     /locus_tag="JNY02_000638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432297.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            5319..5525
                     /locus_tag="JNY02_000639"
     CDS             5319..5525
                     /locus_tag="JNY02_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432298.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            5837..6322
                     /locus_tag="JNY02_000640"
     CDS             5837..6322
                     /locus_tag="JNY02_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBB9432299.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            6303..6581
                     /locus_tag="JNY02_000641"
     CDS             6303..6581
                     /locus_tag="JNY02_000641"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432300.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            6574..8034
                     /locus_tag="JNY02_000642"
     CDS             6574..8034
                     /locus_tag="JNY02_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB9432301.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            8068..8877
                     /gene="murI"
                     /locus_tag="JNY02_000643"
     CDS             8068..8877
                     /gene="murI"
                     /locus_tag="JNY02_000643"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBB9432302.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(8996..9690)
                     /locus_tag="JNY02_000644"
     CDS             complement(join(8996..9444,9444..9690))
                     /locus_tag="JNY02_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBB9432303.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(10214..11587)
                     /gene="eutA"
                     /locus_tag="JNY02_000645"
     CDS             complement(10214..11587)
                     /gene="eutA"
                     /locus_tag="JNY02_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBB9432304.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(11562..12041)
                     /gene="eutP"
                     /locus_tag="JNY02_000646"
     CDS             complement(11562..12041)
                     /gene="eutP"
                     /locus_tag="JNY02_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBB9432305.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIVVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(12054..12389)
                     /gene="eutS"
                     /locus_tag="JNY02_000647"
     CDS             complement(12054..12389)
                     /gene="eutS"
                     /locus_tag="JNY02_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBB9432306.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(12682..14961)
                     /gene="maeB"
                     /locus_tag="JNY02_000648"
     CDS             complement(12682..14961)
                     /gene="maeB"
                     /locus_tag="JNY02_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBB9432307.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            15250..16200
                     /gene="tal"
                     /locus_tag="JNY02_000649"
     CDS             15250..16200
                     /gene="tal"
                     /locus_tag="JNY02_000649"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBB9432308.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            16220..18223
                     /gene="tkt"
                     /locus_tag="JNY02_000650"
     CDS             16220..18223
                     /gene="tkt"
                     /locus_tag="JNY02_000650"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBB9432309.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATCLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(18326..19393)
                     /locus_tag="JNY02_000651"
     CDS             complement(18326..19393)
                     /locus_tag="JNY02_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBB9432310.1"
                     /translation="MNFWGRTVHALSHFPSLFFALLPTSLVWAAPAQRAFSDWQVTCN
                     NQNFCVARNTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGE
                     PLALSGDKWRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFI
                     DAQQKRVGSETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWR
                     MNGLRCSLDPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRL
                     RLPFNNGQETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYA
                     AEPTCDNWHGPDARPTLWITR"
     gene            complement(19494..20069)
                     /gene="nudK"
                     /locus_tag="JNY02_000652"
     CDS             complement(19494..20069)
                     /gene="nudK"
                     /locus_tag="JNY02_000652"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBB9432311.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(20137..22105)
                     /gene="aegA"
                     /locus_tag="JNY02_000653"
                     /pseudo
     CDS             complement(20137..22105)
                     /gene="aegA"
                     /locus_tag="JNY02_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            22311..24028
                     /gene="narQ"
                     /locus_tag="JNY02_000654"
                     /pseudo
     CDS             22311..24028
                     /gene="narQ"
                     /locus_tag="JNY02_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            24230..27343
                     /gene="acrD"
                     /locus_tag="JNY02_000655"
     CDS             24230..27343
                     /gene="acrD"
                     /locus_tag="JNY02_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBB9432312.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27442..27501)
                     /gene="ypfM"
                     /locus_tag="JNY02_000656"
     CDS             complement(27442..27501)
                     /gene="ypfM"
                     /locus_tag="JNY02_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBB9432313.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            27882..28238
                     /locus_tag="JNY02_000657"
     CDS             27882..28238
                     /locus_tag="JNY02_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBB9432314.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            28242..29369
                     /gene="dapE"
                     /locus_tag="JNY02_000658"
     CDS             28242..29369
                     /gene="dapE"
                     /locus_tag="JNY02_000658"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBB9432315.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            29397..29597
                     /locus_tag="JNY02_000659"
     CDS             29397..29597
                     /locus_tag="JNY02_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBB9432316.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(29707..30405)
                     /gene="ypfH"
                     /locus_tag="JNY02_000660"
     CDS             complement(29707..30405)
                     /gene="ypfH"
                     /locus_tag="JNY02_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBB9432317.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(30479..32494)
                     /gene="tmcA"
                     /locus_tag="JNY02_000661"
     CDS             complement(30479..32494)
                     /gene="tmcA"
                     /locus_tag="JNY02_000661"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBB9432318.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCAGFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(32509..33372)
                     /locus_tag="JNY02_000662"
     CDS             complement(32509..33372)
                     /locus_tag="JNY02_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBB9432319.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(33540..34253)
                     /gene="purC"
                     /locus_tag="JNY02_000663"
     CDS             complement(33540..34253)
                     /gene="purC"
                     /locus_tag="JNY02_000663"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBB9432320.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVAHRLGVQLD"
     gene            complement(34466..35500)
                     /gene="bamC"
                     /locus_tag="JNY02_000664"
     CDS             complement(34466..35500)
                     /gene="bamC"
                     /locus_tag="JNY02_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBB9432321.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(35517..36395)
                     /gene="dapA"
                     /locus_tag="JNY02_000665"
     CDS             complement(35517..36395)
                     /gene="dapA"
                     /locus_tag="JNY02_000665"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBB9432322.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            36541..37113
                     /locus_tag="JNY02_000666"
     CDS             36541..37113
                     /locus_tag="JNY02_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBB9432323.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            37113..37583
                     /gene="bcp"
                     /locus_tag="JNY02_000667"
     CDS             37113..37583
                     /gene="bcp"
                     /locus_tag="JNY02_000667"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBB9432324.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            37836..38453
                     /gene="hyfA"
                     /locus_tag="JNY02_000668"
     CDS             37836..38453
                     /gene="hyfA"
                     /locus_tag="JNY02_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBB9432325.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            38453..40470
                     /gene="hyfB"
                     /locus_tag="JNY02_000669"
                     /pseudo
     CDS             38453..40470
                     /gene="hyfB"
                     /locus_tag="JNY02_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            40481..41428
                     /gene="hyfC"
                     /locus_tag="JNY02_000670"
     CDS             40481..41428
                     /gene="hyfC"
                     /locus_tag="JNY02_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBB9432326.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLLTLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            41445..42884
                     /gene="hyfD"
                     /locus_tag="JNY02_000671"
     CDS             41445..42884
                     /gene="hyfD"
                     /locus_tag="JNY02_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBB9432327.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            42896..43546
                     /gene="hyfE"
                     /locus_tag="JNY02_000672"
                     /pseudo
     CDS             42896..43546
                     /gene="hyfE"
                     /locus_tag="JNY02_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
     gene            43551..45131
                     /gene="hyfF"
                     /locus_tag="JNY02_000673"
     CDS             43551..45131
                     /gene="hyfF"
                     /locus_tag="JNY02_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBB9432328.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            45121..46835
                     /gene="hyfG"
                     /locus_tag="JNY02_000674"
                     /pseudo
     CDS             45121..46835
                     /gene="hyfG"
                     /locus_tag="JNY02_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            46845..47390
                     /gene="hyfH"
                     /locus_tag="JNY02_000675"
     CDS             46845..47390
                     /gene="hyfH"
                     /locus_tag="JNY02_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBB9432329.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            47387..48145
                     /gene="hyfI"
                     /locus_tag="JNY02_000676"
     CDS             47387..48145
                     /gene="hyfI"
                     /locus_tag="JNY02_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBB9432330.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            48138..48551
                     /gene="hyfJ"
                     /locus_tag="JNY02_000677"
     CDS             48138..48551
                     /gene="hyfJ"
                     /locus_tag="JNY02_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBB9432331.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            48581..50593
                     /gene="hyfR"
                     /locus_tag="JNY02_000678"
                     /pseudo
     CDS             48581..50593
                     /gene="hyfR"
                     /locus_tag="JNY02_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            50615..51463
                     /locus_tag="JNY02_000679"
                     /pseudo
     CDS             50615..51463
                     /locus_tag="JNY02_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(51501..52562)
                     /locus_tag="JNY02_000680"
     CDS             complement(51501..52562)
                     /locus_tag="JNY02_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB9432332.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            52775..54238
                     /gene="bepA"
                     /locus_tag="JNY02_000681"
     CDS             52775..54238
                     /gene="bepA"
                     /locus_tag="JNY02_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBB9432333.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVLQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            54259..54618
                     /gene="arsC"
                     /locus_tag="JNY02_000682"
     CDS             54259..54618
                     /gene="arsC"
                     /locus_tag="JNY02_000682"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBB9432334.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(54751..55497)
                     /gene="hda"
                     /locus_tag="JNY02_000683"
     CDS             complement(54751..55497)
                     /gene="hda"
                     /locus_tag="JNY02_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBB9432335.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(55547..56836)
                     /gene="uraA"
                     /locus_tag="JNY02_000684"
     CDS             complement(55547..56836)
                     /gene="uraA"
                     /locus_tag="JNY02_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBB9432336.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(56922..57548)
                     /gene="upp"
                     /locus_tag="JNY02_000685"
     CDS             complement(56922..57548)
                     /gene="upp"
                     /locus_tag="JNY02_000685"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBB9432337.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            57873..58910
                     /gene="purM"
                     /locus_tag="JNY02_000686"
     CDS             57873..58910
                     /gene="purM"
                     /locus_tag="JNY02_000686"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBB9432338.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            58910..59548
                     /gene="purN"
                     /locus_tag="JNY02_000687"
     CDS             58910..59548
                     /gene="purN"
                     /locus_tag="JNY02_000687"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBB9432339.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            59720..61786
                     /gene="ppk1"
                     /locus_tag="JNY02_000688"
     CDS             59720..61786
                     /gene="ppk1"
                     /locus_tag="JNY02_000688"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBB9432340.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            61791..63332
                     /gene="ppx"
                     /locus_tag="JNY02_000689"
     CDS             61791..63332
                     /gene="ppx"
                     /locus_tag="JNY02_000689"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBB9432341.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(63371..>64479)
                     /gene="pdeF"
                     /locus_tag="JNY02_000690"
     CDS             complement(63371..>64479)
                     /gene="pdeF"
                     /locus_tag="JNY02_000690"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBB9432342.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        15753 a        16534 c        16336 g        15856 t
ORIGIN      
        1 tattgttgga ataaataaat atcaatgact taaagccgtg tttgcaacag ggtggatcgg
       61 ggatcgaaaa taggatcaac acaaaaacac cctggaggct acagcacact agggggacac
      121 aaaaacaaca caggaaaaaa taccatgtac caatcagcaa ccgcctacgt ggcgaaacca
      181 ggcgaaacac tggtcaaaca caaaattgca acactccaag cacttcaaac actccaagcc
      241 cttccggaac aggttcaggc cgcctttcgt gatggcatga acatcatccg cttcgatggg
      301 tatcgctggg aatacaagcc agcaactgga ttcgtgcgca tagatcaaat cttgtcgcca
      361 gccaatgaag agccagcgct agccaatgca gaaccagcgc aggagcagga gcaggagcag
      421 gaacaggaac aggaacagaa taaagcggcg gctattgccg agttcaagga gctgcaaaga
      481 caacaaatca cagcgcagca agaagaatgc cgccacccgc tggcaatgcc caagccagta
      541 gcgaaagcca acacagacaa gccgacttca tggtttaacg aagaagcgga agaagatttg
      601 cgttttgata gtaaagagga gcagttgcct gaagagcggg aacaggtgga aacggaagac
      661 cagcaaaacg attatcgcaa ccttccagcc gtcaataacg ctatggctta cctcttcaaa
      721 agcgggctgt ttcacaagct gaaaggcgat gagttaaagc tatatacgtg gatggtatcc
      781 aagtgcaggt atcgcggtaa cgacaaatat gagatttggt attcccgcac caaaatggca
      841 aaagcgttag gtaagagcgt tgacacgata gatcgccacc tggaagcgtt gagaaagctg
      901 ggacttatcc aggaaatgcg ccaggcactg aggggcaaat gtggaaaaac ctacagactg
      961 cgaatcccgt caaaaggcac agcagaagag gcctcaatga cggcacagca gcacgaaaga
     1021 gaagcaaaag tcagctacct ggtacactag gcagcgggtc agcaaagggg ggcaaatgcc
     1081 ccttttttca tgtcagataa tcagatcatt acctcaataa actaataatc acatcgcggt
     1141 tacaaccagt ttgatagtga tagctcaaac ccagacgtgg cgctggctgc aagcaaaata
     1201 agccgcaaaa atgcggtcct atttgccaga accttttatt attcaattgg ttagtgcaag
     1261 aaaaagcgtg attactgttc aagaagtatc cagaaaggtc atgattttgc catgattttg
     1321 acgctgaaaa aaaataggtg ggcatttttg cccactcact agccgcaaat ttgcccactc
     1381 actagccgca tttttgcggc ccaaagtaat aaatacagag aaataataaa tacagatcag
     1441 cgccaagatt tttcgtcttt tcggcggaat caagagacta gtgcttacgt cctgataacc
     1501 tccagagcag accttaactt ctgtgcagca ggactacgct cattgccgct cggctaggtc
     1561 aaatcctcac gataaagctg ttcggattgt gaccataatg ttaaaggact ctttaaatca
     1621 ttccttgacg aattaactag ctggcttaag cggaatagca tgcaggataa aaacaaaacc
     1681 agcttagcag tggggagagc ttcgcttcgc tcgtctcctg gggccaatgc tcaatgaaac
     1741 gacgtaaggc acggagcgct caccataggt agacacataa acatctgaga gcgcaccttc
     1801 tatcgcgcta cgcgctcaat aaaaaccaac gacaccgcca cactgcaaga ccaatggttt
     1861 ttatcgcgta tacagaccag acaggaaaca gcagcaaagc agcgtatagc caggtgcaga
     1921 cagcacactc acacgacgca cagcaggctt aagtgatcac tcaggaaact ttatccccgc
     1981 cgccgatcac cgcgaactat catgaccgcg ccaaattcga aaggaatggc ccatagaacg
     2041 cgatgaacac acataagcgc taacttgctt acctgaaaac ttaacgtcgc cacagagcga
     2101 ttgagagcgc ttaaaatcga tgcgatttca ttaaattttt ctcactcgcc tgaatggtgg
     2161 cgatcaggac tgatcgcaac attgccccgc ctcgatattg agcgcataat atacacatac
     2221 attaagcgga aaagagataa gtttattgtg aaaatcaggg ggttacacat catgcgcata
     2281 tcagaacaac aacggcagat cctcgactat ctggcaaaaa acgaatcaat caccgcacga
     2341 caagccgcag atctcgttta tggtggcaat gttacccgcg tccaggttga cgccgcccgc
     2401 cgctcactca tgacgatggt tagtaatggg ctaatcagga aacaaggccg cgctttcgta
     2461 gctgaaagac atatcaatca cgcttatagc caggacgttg aagaattgcg ccgccagatc
     2521 ctttgggtat tcagcaaaca gggtcaggag tatttccttt ctgaacaccc aaatttcgcc
     2581 gccaaagagt taacagtaag cggattgtcg tgcgcactgg attatcttgt cggtgaaggt
     2641 tgcgttactt acaagcagct atggcacgcc ctgaatacgc ttgtagaaga tggtttactc
     2701 attgccacgc gtgagaacat cgaatactgg ataatgccgc gttcaggtcg ccgcaatcat
     2761 gcacggtata tttcaggcaa cagatacaga acgccggact ttaatccgac acaggaagat
     2821 cgggaccgtg ccgaaatgat gtgcagggaa taggagaaaa gaattatgga taagttattt
     2881 tcgatgcagt gaacacaagc gggcgcaatg cccgttttta ttgccttatt ccacgcctga
     2941 cgaatcaatt tgatcacacc tgagcaaatg catagccaga agttaaggcc gttatattgc
     3001 gattgggagc gttttaaacg gtatcaattt aattaattct gatgtggtga tttgcaggca
     3061 aaaaaaaccc gccggagaag cgggaaatag agacatatcg ttgtagttat taacagatca
     3121 ctttgaggtc gagggcggct aaccctcgct aatattatcc cgccattacc ttgcgggtgc
     3181 aaataatctt ttattgcttt gatgatttaa taaatagcac tttttgctaa caaaaaccgc
     3241 tgtcaatata tggccaaaaa tgtcaacaga aaaaataagg tttataatat taagtatcac
     3301 atgggtaatg ggttcaggga atcggaatcc accgcctcac ggaaattcaa aatttgcttc
     3361 ttctttcctc cgtggggcgg cttctttttc gtatcgaggt atttatgaaa gcaataacat
     3421 tagaacagtt cgcagcaatg gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa
     3481 aaccatcaac cggagtccct ttgccattta ccccacgcgg tactcgccaa ttattctggg
     3541 agcacgaatg caaagaatgg ttgtacagaa cagaacgata ataatcacgc atcacatttt
     3601 gaggtaaaca tcatgaccaa tatcgaaaga ttagaacgct gtgccgatat tatgcggcgc
     3661 agatggattt atgaccctga agcgggttta ttgtttagcc gtgaaacaaa agacgttatc
     3721 aaaggcagca tgaccaataa agggcatttg gttgtaacgg tacataaagg taaattcaaa
     3781 gtgacactca cctatcagaa agcctgctat atatacacat acggcgctta tgacgagaca
     3841 ttatatgaag tgcaccacgt taatttggac aaacaggata accgcatcaa gaatattcgc
     3901 ctgatgccga aagaaaagca ccgccggata cataaaaacg tccgcaaact tattcggctg
     3961 ggccacacac aatggcttca accgcaatta attgaacaat cactataaac acagtagtaa
     4021 ataccatgca ttcattagac agttattttc agcgcacgac agcccctaaa tcagccgccc
     4081 aggaacgccg tgaggaattc catgagaaag tgatgcgttc tgctgattat attgcggaca
     4141 agttcgtgga aacggttcgt ccacttgttg atgaagtagc tgataaactt cagtctgaaa
     4201 tgccggaaga tatggaaggc actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt
     4261 ttggcgtgtc aataagcgct tttaaataat cgaaaaaagg ggggaaagat aatggacgca
     4321 tttgaggtac tggcaaataa aggcgctgaa ctggtagcac agcgtgataa aaccgccaac
     4381 gaaggggaac gcgcactgct aaataaacag attaaagcga tccgtatggc ccagtttaaa
     4441 ttaatcagta atgaagttat cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac
     4501 gctattaaag ccgccggact ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa
     4561 atggagatgc acaaacagag cggtgctccg aatcccgatg agtacttctc accggatgtt
     4621 gagtttatgg cgcaggttga ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc
     4681 tattttgatg gagtggatag ttcggcaggt attgacctga actcgtattt cggtcgtgaa
     4741 attgagcatt ttgatgccgc cccgtctatg ccggagccag aaccagaaat ggccaccgat
     4801 cctgaagctg agcaacggaa acgcgtattc aatgcgatag ggattcggta ctgatcggat
     4861 aaaagaaagc cgggaattac cccggctgac ttaacaatgt gaccaatgaa caaatcggac
     4921 aagacaaaca ggataagcag tctcgaaaca atgttcggga actatattag cacaagtccg
     4981 gagaaaatca tcatgttggc actacgcatc atgcagggca tcgctaagac cctcgcagaa
     5041 catgtcttag acctgaaaca ttcgccactg agcaaacagg ccatgaagcg ctaaacgctt
     5101 cgtctgtggg ctgaatattc gcttggaact atcaataaaa tcatcgacat gaaatcgggg
     5161 ccgagcaacc agagcgcgga ggaaatggag ttcatcaggc gtttaattct cattcgcaga
     5221 gacattcata gccagttaca cagcgtggga atcgatatta atgatgggac gggcgattaa
     5281 ttttcggaca agtactatgc gcgcgcgagg agctgaacat gaagcaagat attgcagaca
     5341 ggctggagat actcgaaggc cagcgggcag aagcgaaaca actacgtaag caggccagaa
     5401 gagcgcacag gaacaacgaa gcagagttac taaccaaata catcagtttc accaattact
     5461 gcatctacga gtgttacaag gaagatgcag aggactggct ggattcattg ccagaacagt
     5521 actgagcaga ataacaccac gtcatgttgt tctgaggtga gcatatcacc tctgttcagg
     5581 tggccaaatt tagtgtgcca cttcagcatc accgttgaaa tcacaataac gatttttttg
     5641 taaatatgct ctcacatatg taggaataga ccaggttggc attgaaacac atctaccatg
     5701 accataataa agtaattttt tgatatgttt atggttttat attaattaaa atatatctaa
     5761 aattgatagt tttaaaattg caaaatccag attgtgtttg taatgtttaa tctgattgta
     5821 ttgagtggcg tttattatga aaaataaatc aggtaattct tgttatagag aagcagtcga
     5881 tattcatagc tcacttgagc tttcatttat attgcataaa tatcatttgc gtgtagatga
     5941 gaagaaatct cgaaatgtat tcagcgccat acgtgatgag ctcatccttg atggggattc
     6001 acgacaaaac ttagctactt tctgtcaaac atggcttgat gatgaaatcc gtgagttgct
     6061 taatttagct attgataaaa acatgattta taaagatgaa tatccacaaa tggcggaacc
     6121 tgaaggtcgc tgtgtgcgta tgctttccga ttcgtggagt ttccccgatt cgcgacacac
     6181 cttaggttgc tcaaccattg gttcatctga agctgctatg ctcggccgct tggcgttaaa
     6241 atggcagtgg tgtaaaaaac gcgaagtaca aggtaaatca accgaacctg atttgcgatc
     6301 ctgtgcaaat atgctggcat aaattctgcc gttactgtga tgtgaaattg tgtgaaattc
     6361 cacttgatga tcatcacctg ataatgaatg ctaaagaggt tttgaagcat gtcgatgaaa
     6421 acactattgg tgtcgttacg actttaggta ttacttattt ttgtaaagca gattgcgtta
     6481 gtgtttttaa gataagtgtt aaagttaata ttagttttta tatttacatc gtaattatat
     6541 gtttctattt taaaaatatt cgagagtata aaaatgtcta atgctaatat cgcacccaaa
     6601 agtaagaaaa tgacagtggg gacactcgct atcatgaata taactgctgt agtaagtcta
     6661 cgatttatac cttctgaagc agaatacggc ttaggtgcca ttttttacta tacctttgct
     6721 gcaattatgt ttttagtacc tgttgccttt gttgcggctg agctagctac aacatatcca
     6781 caaaaagggg gggcatttcg atggatagga gaagcatttg gggctcgctg gggttttgta
     6841 ggaatgctta tgacctggct acaagtcatc ccctactttc caacagtact gacatttggt
     6901 gcagtctcgg ttgcatttat tgatgttgat atgggggtgg cagaatcaat tgccagtaat
     6961 aaatggtata ttttattttt cgtactattt gtttactggg gagctatttg tgttgcatta
     7021 cgtggcgttg gtattttctc gcaggtatct aagtggtgcg gtattatagg gacaattatt
     7081 ccagctatta tccttgttat tcttggtttc tcctatctgt ggttcagcgg taaaccagcg
     7141 gtgatcccat taggttgggg tgatcttttc cctaatatag ctaacttcca gaatattgtt
     7201 cttgccgcca gcatatttct tgcttatgca gggatggaaa tgaatgcggt ccatgttaac
     7261 gatttagata atccaactaa aaaataccca attgcaatta ccatcgcttc tataggtact
     7321 attgcaatat tcctgctcag tactttaggg gtcgccttta ttattcctac agataaaatt
     7381 agtctaactc aaagtttgtt ggttgcctac gatatgctgt tcgaatgggc tggagtgcca
     7441 tggcttggct ctgtgatggc atttatgcta gcaattggcg tactgggtgg ggtggttact
     7501 tggattgcag ggcctaatac aggtgtatta gcgatcgcga aagctggtta tttacctaag
     7561 tttttccaaa aaaccaatcg tcatggaatg gggcaccatc taatgtttgt acaaggtata
     7621 attgtctcag ttctttctgt tacttttgtg ataatgcctt cagttcaagc agcttttcag
     7681 attttatcac agttaacagt tatgctctat ttagttatgt atatgctcat gttcgctagt
     7741 gctatctatc tacgacattc tcaacctgat acaaaacgcc catatcgagc ccctgcttta
     7801 tttttctggg caacacttgg attcttaggg tcttttctgg cttttgtatt aagttttatt
     7861 ccgcccgagc aaatcccaac tggtagtcca accagctatg ttctttattt gttagtatta
     7921 gttgccctat ttttattaat tccaatatta atttacgcct tccgaaaatc tgactggaaa
     7981 gatccaaact ctgatttcga accattttct tgggaaaaaa gaactagtaa ttaaattttc
     8041 cataagctac aaaagatgag gttaaatatg tttagtgctg aatctccaat tggtgtaata
     8101 gactcaggtg taggtggatt gacatcggta aaggaaataa ttaatttgct tcctggtgaa
     8161 gatattatct attgtggaga taatatgaat gcaccttatg gcaaccgttc tgccgatgat
     8221 atcatcagtt tgactaaaaa aatgctgact tttttacagt caaggaatgt caaactagtt
     8281 gctgtcgcat gtaatactat atcctcaact ttggaatccg aaaaatacag cggatatgct
     8341 aagagctttc cgtttcctat cttatccatc attgaacctg cagtagaaga tgtgattcgc
     8401 cagcaatata aaaatgttgg tattatcgct accgagttta ctattaaaac ggggtgtcac
     8461 aaagagctaa taaaaaaatt gaattcaaca ataaatgtat ttggagaacc aagcaaaaat
     8521 cttgcaatgt taattgagga aggtaatctt aatgctcctg caatcttgaa tgacatcaaa
     8581 aaacatgtta accatttaat ttccctccat ccagtaaatg aaattattct cggctgtact
     8641 cactatccaa ttgttcagaa tttttttgaa gcagttgcac cggatataaa atttattaat
     8701 cctgcacatg atcaggctat ctctattaaa aatcacttgg gacagctaaa tttattaaac
     8761 tcctcaaata taggaacttt gcaaataaat actagtggaa gtatggagat ctacaaaacg
     8821 gtactagggg aattgagcat tacgaaacct catacattca gtatcaggca gttttaaatt
     8881 tatccggtgc accgtaaaat cccgtccttc tgaactgcac cccaaaagtt ggacaccaac
     8941 tgagtaaggt gcattttatg gacaaaccca gttacatact gggtagtgca tccaattagt
     9001 agaacatgtg tttttcgata aacgctccga tcactttttc gtggatctca actgagcgtg
     9061 agaagcagat tgttttacga cccaaccgct taatgcgggt gcgtagcgtc agattattac
     9121 gctcaatgcg ttgggtgaat attttgccgg tcagatgctt attcttcggc acctcccggc
     9181 catagctgcc ccagtcatcg ctggtcagca tgccgatgtt gaagggtgta agcagtgcca
     9241 gtagctcccg gcacgtttga tcggttcggg gaccaaaagt gtaggccagt acaccgcctg
     9301 ttttggtgtt gtacgcgtac ccgagccaat gttgccgggc tttactgcca acgtagctcc
     9361 attgctcatc aagctcgcag ataaacgcca catcagcatg ggcaacaggc gaagaagtta
     9421 ttcgctttgg cgcgagtttt ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt
     9481 cctggcggta tcgcgaaccc cggcaccatt aaaggccatt tcagttatca gctctttcat
     9541 acccggctta cgtgcttgat aagtataagt gaactgaaac acacggtggc agtcacggca
     9601 gcgaagtctg tcacggcctt tagggttctg accatggcgg taaacctgag ctgactgaca
     9661 acggggacaa tgaatattaa cgctggtcat gagataacct caaaagcccg tattatacat
     9721 cagattcaac taattagagg catcacccat ccagcattat aaaacatact aacaataact
     9781 aaattataat aatcacaact tacaatcaaa taataaatgc ataagattaa caatgatttt
     9841 ttcgggttat atttatctaa aaaagcagaa tattatgtaa cttattattt tctaagcgaa
     9901 aatttattgt gcggtcagac taacaaactt gcgtaccacc cgaagaacat gcgaagattt
     9961 aagtattctt tcttttttgg tttttatttt tcattttctc ttttcttctc tttccggttc
    10021 tcttctgttc tggttttggt tttttccttt ttatttttat tctttcttca gggttatgct
    10081 aaaacaccaa aacatttttc accacaaaag catccaatca tcttgccaga ccaatcacct
    10141 taaccggata cactgccgga tataatccca ctaacaccca gccaccaagc accatacctg
    10201 ataactaccg tattcaggaa ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc
    10261 cgccaaaaag aggcgtacca atgtcgatat agtcccccgc gcggacaatc acttcgtcaa
    10321 tgactgccaa cgggagttgt tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa
    10381 agtcctgccc ggccaccacc agcaggggat gcggattcgg aaaacgcgcg acgaaatcga
    10441 ccagcgcgtt gatgaccgtc agtaccgcgg cgtaacgcac aggcagcgag gcgggaagcg
    10501 ccagcacgta cacgtcagtt ttgggatcaa gatccagctg aagcagcgcc tgttgccagg
    10561 cactcaccag atccgtttca tcaatcggga tcgccaccgg caaattgcgc agcggcagtt
    10621 gtacgccctc cagccagatt gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa
    10681 tcaccgtggc gcatacggtt tgcgccggaa actgcacatt catctcacgc aggcgcggat
    10741 ggtcatgcag cgccgttgcc agaagtgggc caatatcggc aaaacagaac gggtcggcgg
    10801 gctggtggcg ataacattcg cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac
    10861 ctgcgggcag caaaccggtt tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa
    10921 tcacttcgac aatcagcgcc gccatccgcc gggtaacctg caccagctgc gcgccggtca
    10981 gcgaacgggc gtcagtgcct gcaccgaagc actcatccac aatcatctgc cccggtttat
    11041 gagcgtaaac cacgcgcccc tggctgtcgg tttccagcag gcgaccaccg acgttgagac
    11101 aggcagtgcc gctgattttt ccggcatcga acagggcgta gttcgcggtg ccaccgccga
    11161 tatcgatatt cagtacccga cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt
    11221 gaccggcgat cacggattcg aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg
    11281 actgtttttc cttcatcact ttttccatca gctgtgcgac caggctttcg gtaaactggc
    11341 cttccggcag ctgggcaatg atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg
    11401 gcacgctggc gggcacggac gccgttaccg ggatcgattc atcacattcg gtgatggtaa
    11461 aacccagtag atccgacact tcacgggctt ccggggtaat aatgctggcg cgcagaacca
    11521 cggacattgc ctgttcgccg cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt
    11581 ttttcacctg cttcctcctg tttggtgaga gatgccagat aatccaccag ttgctgcacg
    11641 ctttgcgggt cgtggctgtt tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat
    11701 aactttcgcg ttgcggcgac gtcagcatct ggcatgtccg ttttactgat gacgacgatt
    11761 tgccgtttgc tgacgccaat atccagcaac ccggcaggca accgactttc cgggtcattt
    11821 gcgccatgga catagatcag catgtctaca tcctgtagcg tggtaattaa ggcgtgatac
    11881 cagcggggat ggctaaaata ttcacccggc gtatcaatat cgcccttatc attaaattcc
    11941 acggcctgtg tttttctggc gagggtataa ttcccctgca acgcattaaa taacgttgtt
    12001 tttcctgccc cgacgctacc cacaaacgca attcgtttca tgaatgcctc gtattaactt
    12061 ttggtcattt cacaaagcgt ataatttaat aagcgaccta aaccgctgac ggtttgtgat
    12121 aacgcttctt ctactgcgcc caccgagcca taaatcacca atgcaccgct gaagcggtcg
    12181 agaaaaccga tatgcacatc agcggctttc agcgctaaat caccggcgat catcgccgtt
    12241 tcgccgggag ttagcgtcat aatgccaatt gcgcccgcat ccggaacgcc gattttcttc
    12301 gccagttcct caccgggatg cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca
    12361 aattcctgaa tgatgcgttc tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat
    12421 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
    12481 tagatcacta agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
    12541 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
    12601 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
    12661 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
    12721 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
    12781 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
    12841 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
    12901 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
    12961 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
    13021 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
    13081 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
    13141 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaaca aaggtgttac cactcggcag
    13201 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
    13261 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
    13321 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
    13381 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
    13441 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
    13501 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
    13561 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
    13621 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
    13681 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
    13741 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
    13801 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
    13861 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
    13921 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtcaagcg cgccacggaa
    13981 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
    14041 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
    14101 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
    14161 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
    14221 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacgccc
    14281 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
    14341 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
    14401 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
    14461 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
    14521 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
    14581 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
    14641 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
    14701 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
    14761 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
    14821 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
    14881 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
    14941 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
    15001 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
    15061 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaacaat cctataacac
    15121 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
    15181 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
    15241 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
    15301 ggcgatattg agtccattcg ccattatcat cccctggatg ccaccaccaa tccttcgctg
    15361 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
    15421 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
    15481 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
    15541 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
    15601 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
    15661 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
    15721 ttttcttttg cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
    15781 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
    15841 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
    15901 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
    15961 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
    16021 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
    16081 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
    16141 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
    16201 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
    16261 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
    16321 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
    16381 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
    16441 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
    16501 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
    16561 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
    16621 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
    16681 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
    16741 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
    16801 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
    16861 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
    16921 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
    16981 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
    17041 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
    17101 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
    17161 aaaggctcat ccgcaactgg cagaagagtt tacccgacgg atgagcggtg gtttaccgaa
    17221 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
    17281 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
    17341 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
    17401 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
    17461 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgtgcct
    17521 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
    17581 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
    17641 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
    17701 tcaggtggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
    17761 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
    17821 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
    17881 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
    17941 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
    18001 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
    18061 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
    18121 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
    18181 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
    18241 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
    18301 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
    18361 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
    18421 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
    18481 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
    18541 cctgaccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
    18601 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
    18661 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
    18721 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
    18781 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
    18841 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
    18901 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
    18961 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
    19021 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
    19081 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
    19141 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
    19201 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
    19261 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
    19321 acgtcggcaa caaagcgaaa aaaagagaag gaaaatgcga tagcgcatgg acggtacgac
    19381 cccagaagtt caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa
    19441 tcggatttgt cggattgagc caataataaa caacgaaata ttattgctat ttttcagtcc
    19501 attaaatgtg acatttgcaa atagttaagc aataacaccg tcttaccgtc acgtatctcg
    19561 ccggttttga tcatctccag cgcctggctg aacggcagct cgagcacttc aatatcttca
    19621 tcttcaacac cgccaccggc gttagcgcgc tgattgtcac tgtattcggc gataaaaaag
    19681 tggattagct cagtcacacc gccaggcgac atatacagtt caaataattt gcgcacttcg
    19741 cccacttcat agcccgtctc ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg
    19801 ttatccagca gcccggcgca ggtttcaatc agctgcccgc tttcattgcc attaacccag
    19861 gtagcgacac ggaactgacg aatcagaacc acgctctttt tcttcgcgtt gtacaggagg
    19921 atcgtcgcac cattaccgcg atcgtaaact tcacgtttat ggcggataac ttcaccatct
    19981 ttgcgcgtga gatcgtaagt aatgttgtgc agggtgaaat agttatcgga gagaattttg
    20041 tctttaatga gggtgatttg ttgcgtcata ccgactccac agcgcgaaat gaacaattat
    20101 attacgctgt gaagtcgggt ttgtctgcgc aggctatcag tgagatttga ctgattttac
    20161 ccccagccag tcaataatcc cctgtgccgc atgacgtcct tctgccattg cggtaaccac
    20221 cagatccgca ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg tggtctggta
    20281 acggtactgg ctttccacat ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg
    20341 cgactccagc cacggcatcc cgtgtggatt gaagccaaac gccataatca ccgcatcggc
    20401 tggcatgaca aattcactgc cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg
    20461 ctcgccaaga cgcgtgcgca ggaaacgaat tccgcaaacg tgaccttgct cattcagctc
    20521 aagcgccacc ggctggacgt taaattcgaa gttggccccc tcttcgcggg cgttcttcac
    20581 ctctttcttc gagcctggca tgttagcttc atcacgacga taagcgcagg tgacgttact
    20641 cgcgccgtgg cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac
    20701 cacgacgtta agtccggcgg tattgataaa cggctcttcc ggtagctctt cgagccccat
    20761 cacctgttta gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg gcgtatcttc
    20821 attgggtaaa cccgctttca tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta
    20881 ttgttccaaa agcgaatcca aagagacatc tttacccact tcacaattga gttcgaagtg
    20941 aatccccatc gcgctgaaga tttcccgacg gcgtgccagc agggatttat ccagtttgaa
    21001 agaaggaatg ccgaaagtga gcaagccacc gatttctgga tggcgatcgt acaccgtcac
    21061 cgccacgcca ttgcgggtca gaacatccgc acaggccagc cctgccggac ctgccccgat
    21121 aatcgccacc cgcttgtcca ctttggtgac atgacttaag tcaggacgcc aacctttcgc
    21181 caacgcctga tctgaaatat agcgttcaat gttgccgata gttaccgcgc cgtgctcatc
    21241 gcgaatagta caggcacctt cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc
    21301 cggcagggtg ttggtctggt gagaaagctc gacggcggcg tcgatgtttc cggctttcac
    21361 cagttcaatc cactgcggaa tatggttatg cagcgggcag gtccattcac aaacgctgtg
    21421 ctcgccgaac ttaaggcagc gcgaggcttc ccgttgtgcc tggtcgcaga taaatttcat
    21481 caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg
    21541 cctgcatttg ttcgacttta ctcattaccg gcatttcttg tgcagcggta ctggcatgcc
    21601 acggttgatg ctcctgacgt gcggtgcgca agcggcggga tttcgccatg ccggagagtg
    21661 cgacgtcagt gaccagttgc agcgcgtccg ccgggcaatt ctcgacacag gcaggaccgt
    21721 tttcgcgccc tgcacaaagg tcgcatttat gcgccgtggc ttttactttt cctgccgcga
    21781 cgggtgtcag gacgatttgc atcgtaccaa acggacaggc caccacgcag gatttacagc
    21841 caatacactt ttgctgattg acctgaatgc tgtcatcaac gtgactgatt gcaccattag
    21901 ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca ctacgttgct
    21961 gctgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg acatgttgct
    22021 catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga cattgctgac
    22081 tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa aaccttattc
    22141 tttatatgag tgttgttatt acccgactta caggggaacc ggcaatgttc atgtgcccag
    22201 aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca ctgactaaat
    22261 tgcgtttcag cgaactggaa cattaatgat tttttgtgga gaagacgcgt gtgattgtta
    22321 aacgccccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg ctgtcgattc
    22381 tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct gaggctatca
    22441 atattgccgg atcgctgcgt atgcagagtt accgcctggg ctacgacttg caaagtggca
    22501 gtccacaact caatgcacat cgccagttgt ttcagcaggc actgcattca ccggtattaa
    22561 ccaacctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg catctgaatg
    22621 ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg tatcaggcca
    22681 atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag cactacgctg
    22741 aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggtatcggt attttcacgc
    22801 tggtcttttt tactttgcgc cgcatacgcc atcaggtggt tgccccgctg aatcagctgg
    22861 ttaccgccag tcagcgtatt gaacacgggc agttcgactc gccgccgctg gataccagtc
    22921 tgccgaatga gcttggtctg cttgcaaaaa cctttaacca aatgtcgagc gagctgcata
    22981 aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc cacgaggcca
    23041 agcgtcgtct ggaggtgttg tatcagtgca gtgttcccag gcgctgaaca ccagccagat
    23101 tgatgtgcat tgtttccgcc atattttgca gattgttcgc gacaatgaag cagctgaata
    23161 tctggagctg aacgttgggg ataactggcg gattagcgaa gggaagccaa accctgagca
    23221 gccaatgcag attttaccgg tgacaatgca agagacggtt tacggcgaac tgcactggca
    23281 aaatagtcac gtttcatcat cagaaccgct gcttaacagt gtttcatcga tgctgggacg
    23341 cggtttgtac tttaatcagg cgcagaagca ttttcagcag ttattgttga tggaagaacg
    23401 tgcgaccatc gcccgcgaat tgcacgactc gctggctcag gtgctttctt acttacgtat
    23461 ccagttgacg ttactgaagc gttcgatacc ggaagataac gccaccgcac aaagtatcat
    23521 ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag ttacgcgagc tgttgactac
    23581 tttccgcctg acgctgcagc aggcggatct cccctccgcg ttgagggaaa tgctggatac
    23641 gttacaaaat caaaccagcg ctaaactgac cctcgactgc cgtctgccaa ccctggcact
    23701 ggatgcgcaa atgcaggtgc atttgttgca aattattcgc gaagcggggc tgaatgcgat
    23761 gaagcacgcc aacgccagcg aaatcgccat cagttgcgtc accgcgccgg acggcaatca
    23821 cacggtttat atccgtgata acgggattgg tatcggtgaa ccgaaagaac ccgaaggtca
    23881 ttatggactg aatatcatgc gcgaacgcgc ggaacggctg ggcgggacgc tgactttttc
    23941 acaaccttcc ggcggcggca cgttagtgag tattagcttt cgctccgcag agggtgaggg
    24001 gaaggggaac acgccggctg aggtgtaaat aattaccctc acaccggccc tctcccttta
    24061 aaggcgagag ggaaaaacgt gcccatattg acctgtaagc gagtgtgtca gcaatctcat
    24121 gataatttac attaactcct ttttttctcc acgattggct cgtaccttgc cgctacagtg
    24181 aagcaagtca agcctacaac aatacgcaga aacacgaggt cctcttttaa tggcgaattt
    24241 ctttattgat cgccccattt ttgcctgggt gctggcaatc ctgttgtgtc tgacaggtac
    24301 cctggcgatt ttttcattgc ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg
    24361 agtgaccgct aactatcccg gcgcatcggc ccagacgctg gaaaacaccg tgacccaggt
    24421 tatcgagcaa aatatgaccg gcctcgataa tctgatgtat atgtcatctc agagcagtgg
    24481 caccggtcag gcatctgtca ctttaagttt taaagcaggc accgatccgg acgaagccgt
    24541 gcagcaagta caaaaccagc tgcaatcagc catgcgaaag ttaccgcagg cggtgcaaaa
    24601 tcagggcgtg acggtgcgta aaaccggcga taccaacatt ctgaccattg ccttcgtctc
    24661 taccgatggc tcgatggata aacaggatat tgctgattat gttgccagta atattcagga
    24721 cccgttaagc cgcgtgaatg gcgtcgggga tatcgatgcc tatggttcgc aatattccat
    24781 gcgtatctgg ctggacccgg cgaaactcaa cagtttccag atgacggcta aagatgtcac
    24841 tgatgccatt gagtcacaga acgcgcagat tgcggttggg caacttggtg gtacaccttc
    24901 cgtcgataag caggcgctca acgccaccat taacgcccag tcactgctgc aaacaccaga
    24961 acagttccgc gatatcacct tgcgggtgaa tcaggacggc tcagaggtaa ggctgggcga
    25021 tgtcgccacc gtcgaaatgg gggcggagaa atacgattat cttagccgct tcaatggtaa
    25081 gccagcctcc gggttggggg taaaactggc ctccggcgca aacgaaatgg cgacagcgga
    25141 gctggtgctc aatcgtctcg acgagctggc gcagtatttc ccgcacggac tggaatacaa
    25201 ggtggcgtat gaaaccacct cgtttgttaa agcctccatt gaagacgtgg tgaaaacgct
    25261 gctggaagct atcgctctgg ttttcctcgt tatgtatctg ttcctgcaaa acttccgcgc
    25321 cacgctgata cccactatcg ccgtgccggt ggtgttgatg ggaaccttct ccgtacttta
    25381 cgccttcggt tacagcgtca acaccttaac catgttcgcg atggtgctgg cgatcggtct
    25441 gctggtggat gacgccatcg tggtggtgga aaacgtcgaa cgtattatga gtgaggaagg
    25501 actcactcct cgcgaagcca cacgtaaatc gatggggcag atccaggggg cactggttgg
    25561 gattgcgatg gtgctttcgg cggtatttgt accaatggcc ttcttcggcg gcaccaccgg
    25621 tgccatctat cgccagttct ctattaccat tgttgcggcg atggtgctgt cagtactggt
    25681 agcgatgatc ctcactccgg ctctgtgtgc cacgctactt aagccactga aaaaaggtga
    25741 gcatcacggg caaaaaggtt tttttgcctg gtttaaccag atgtttaacc gcaacgccga
    25801 acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc ctgcgctgga ttgtgattta
    25861 tgtcctgctg cttggcggca tggtgttcct gttcctgcgt ttgccgacgt cgttcttacc
    25921 gctggaagac cggggcatgt ttactacctc ggtacaattg cccagcggtt caacccaaca
    25981 acagaccctg aaagtcgttg agcaaatcga gaaatattac ttcatccatg aaaaagacaa
    26041 catcatgtcg gtgtttgcca ccgttggttc tggccctggg ggtaacgggc aaaacgtggc
    26101 gagaatgttt atccgcctga aagactggag cgaacgcgac agtaagaccg gcacctcgtt
    26161 tgccattatc gagcgtgcaa cgaaggcgtt taaccaaatt aaagaagctc gcgttatcgc
    26221 cagcagccca ccagcaatta gcggtcttgg tagttctgca gattttgata tggagttgca
    26281 ggaccacgct ggagcgggtc acgatgcgct gatggcagca cgtaatcagt tgctggcgct
    26341 ggcggcggaa aacccggagc taacccgtgt gcgccataac ggcctcgatg acagtccgca
    26401 gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg ggcgttgata tcgacgatat
    26461 taacgacaca ctgcaaaccg cctggggttc gagctatgtg aatgacttta tggatcgcgg
    26521 tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat cgcatgctgc cagatgacat
    26581 caatctctgg tatgtccgaa ataaagatgg cggcatggtg cccttctctg ctttcgcgac
    26641 ctcacgctgg gaaacaggct cgccgcgtct ggaacgctat aacagttatt ctgcggttga
    26701 gattgttggg gaagccgcac cgggggtcag taccggtacg gcgatggata ttatggaatc
    26761 gttagtgaag cagctgccaa acggctttgg tctggagtgg acggcgatgt cgtatcagga
    26821 gcggctttcc ggcacgcagg ctccggcgct gtacgccatt tccttgctgg tggtattcct
    26881 gtgtctggcg gcattgtatg aaagctggtc ggtaccgttc tcggtaatgc tggtcgtgcc
    26941 gctgggggta atcggcgcgc tgctggcaac ctggatgcgc gggctggaaa acgacgttta
    27001 cttccaggtg ggcctgttaa cggtcattgg tttatcggcg aaaaacgcca tcctgatcgt
    27061 cgagtttgct aacgagatga accaaaaagg ccacgacctg tttgaagcga cgctccacgc
    27121 ctgccgtcag cgtttacgcc cgattctgat gacctcgctg gcatttatct tcggcgtatt
    27181 gccaatggca accagcacgg gtgccggttc cggtggtcag catgcggtgg gtactggcgt
    27241 aatgggcggg atgatttcag ctactattct ggctatttac ttcgtgccgc tgttctttgt
    27301 gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa taagcaataa aaaaggcgac
    27361 atgccaatgt gtcgcctttt tcaactttcc gattaagaac ctgctcagcg ggttcttgct
    27421 gtttatactt tgtctcagga attacttacg aagcataact tcgataaagt ctttccagtt
    27481 ccccagttca cgttcaatca taacaacctc tcttataatt atgggtattc tacggaaaaa
    27541 atataccgtg gtgaagctaa tttactcgat tgctgcgatg actacctccg ggggacaaac
    27601 cttatgtaaa tactatggtc ctgcggtgat aatttgtatg tgatacacag caacatttcg
    27661 agatattcat acggcatcta atacttattt aattctggtt aaaatacaga cagataacaa
    27721 gatgaatatt cttaatgttt acgttaaaaa tgtttaatat tatttaatag ttgttaattt
    27781 gaatacttcg ataatgttat attttctgat aatcatttgc aggcaaaatg ttttcaccct
    27841 taaatgagta tttattctca taaatcgaaa aaggattcat tatggttaca ctttacggca
    27901 tcaaaaattg tgacaccatt aaaaaggctc gccgttggct ggaagccaat aacatcgact
    27961 atcgttttca tgattaccgc gtcgatgggc tggacagcga attattgaac ggttttatca
    28021 acgaattagg ctgggaagcg ttactcaaca cccgtggtac aacctggcgt aaactggacg
    28081 aaactacccg caataaaatc accgatgcgg cctctgcggc ggcattaatg actgaaatgc
    28141 ctgcaattat caaacgtcca ttgctctacg cgcctggtaa gcctatgctg ctgggtttca
    28201 gtgattccag ttatcagcaa tttttccatg aggtgtagtc tatgtcgtgc ccggttattg
    28261 agctgacaca acagcttatt cgccgccctt ccctgagtcc tgatgacgca ggatgtcagg
    28321 ctttgttgat tgaacgtttg caggcgatcg gttttaccgt tgaacgcatg gattttgccg
    28381 atacgcagaa tttttgggca tggcgtgggc aaggtgaaac gttagccttt gccgggcata
    28441 ccgacgtggt gccgcctggc gacgccgatc gttggatcaa tccgccgttt gaacccacca
    28501 ttcgtgacgg catgttattc gggcgcggtg cggcagatat gaaaggctcg ctggcggcga
    28561 tggtggtagc agcagaacgt tttgtcgcac aacatcccaa ccatacgggg cgactggcat
    28621 ttctgatcac ttctgatgaa gaagccagtg cccacaacgg tacggtaaaa gtcgtcgagg
    28681 cgttaatggc acgtaatgag cgtctcgatt actgcctggt cggcgaaccg tcgagtatcg
    28741 aagtggtagg tgatgtggtg aaaaatggtc gtcgcggatc gttaacctgc aacctcacca
    28801 ttcatggcgt tcaggggcat gttgcctacc cacatctggc tgacaatccg gtacatcgcg
    28861 cagcaccttt ccttaatgaa ttagtggcta ttgagtggga tcagggcaat gaattcttcc
    28921 cggcgaccag tatgcagatt gccaatattc aggcgggaac gggcagtaac aatgttattc
    28981 cgggtgaact gtttgtgcag tttaacttcc gcttcagcac cgaactgact gatgagatga
    29041 tcaaagcgca ggtacttgcc ctgcttgaaa aacatcaact gcgctatacg gtggattggt
    29101 ggctttccgg gcagccattt ttgaccgcgc gcggtaaact ggtggatgcg gtcgttaacg
    29161 cggttgagca ctataatgaa attaaaccgc agctactgac cacaggcgga acgtccgacg
    29221 ggcgctttat tgctcgcatg ggggcgcagg tggtggaact cgggccggtc aatgccacta
    29281 ttcataaaat taatgaatgt gtgaacgctg ccgacctgca gctacttgcc cgtatgtatc
    29341 aacgtatcat ggaacagctc gtcgcgtgat gagtggttct gcaagaggaa ataagcatgg
    29401 actggctggc taaatattgg tggattctgg tgattgtctt tttggtcggc gtcctgctga
    29461 acgtaattaa agatctcaag cgcgtcgacc ataagaaatt tctcgccaac aagccggagc
    29521 ttcccccgca tcgtgatttc aacgataagt gggacgatga cgacgactgg ccgaaaaagg
    29581 atcaaccgaa gaagtaagct gagtgctcaa ccgtaggccg gataaggcgt taacgccgca
    29641 tccggcaatg gtgaacgatg cctgatgcga cgctcacgcg tcttatcagg ccaggtaatc
    29701 tgtcttttac atcatttcaa tcacatcatc atcgccaggc ttaccgccac ttagcgcttg
    29761 gtcgaaataa tgtttcggaa tggtataacg caagtgatcc agggcaaatt gcatactgcg
    29821 attgtcgatc gcatggccta aatcctccac gatatccagc gtcacgtcac caccggcact
    29881 gattaacgct tcctgcgccg caactgcatg cgccagatcg ataaccggat cttcaccacc
    29941 atgaatcaag tgaattgtcg tggctgtaga cgcggtctcc ggcaggctcg catagcgtcc
    30001 gttaaaggca atgacgcgcg aagcaaggcc cggttcggct ttaatgctct ccagcgccat
    30061 aatcgcgcct tgtgagaaac caattagcgc cgtggcgtta gcccctactc cgctctgttt
    30121 ctgccagtag cgtaccgttt caataaacgt cggcatgatg gcatccacac gcgcctggcg
    30181 attatcttcc gtaataccct gcactgaaaa ccactgacgc cccgccggat taccgctcgg
    30241 ctccgcgcca ccgacactca ccaccagcgc atcaggaaac agcggtgcaa accagctgcc
    30301 tatttccccc attgccaccg ggttatcccc gacaccgtgg aaaagcagta acaactgttg
    30361 tgcaggttta tccgggcttt gaacaacaaa atggtcatgt ttcatggcga actccttaac
    30421 tgatgtcatt aattttacgc cgctgactca caatgaccat gggagaaaat tgaaggagtc
    30481 agtgaaaaaa ttgccattgc gttatgcgat cgcgcagacg ctcagtgcga acctcattta
    30541 gtgcgaacag cgcctgcgcg gcctcttccc gctggcgaac cagtaacatc ttgcggcctg
    30601 aaagtttaag tgtggtacat aactgcgcgt cactggcgtt tttctgtaaa cgcccacgca
    30661 gcgccggtaa tgccagttca ctagtttgta gcagacgcaa taagcaacct aacgatgtta
    30721 atagcggacg atgagcgaaa gcaaaaccgg ccagttcaag ccagtcgtcg tcagaaagga
    30781 tggcatcgtt tagtggatca acaggaagca tttcgccatt ccactgcgcg agagcttgcg
    30841 catcgcgacg taaacggtaa tgctcacgtt cagccagctg tttacccgca tcactcatcg
    30901 gtaacagcgc catcgccgta tagcaaccgc tgctggcttc ccgatgatta cccatccgca
    30961 ccagcacaaa accgcagcgt tgccagaaac gccataactc cccagtgtaa ccaaaactca
    31021 ccgaaagata gtcgaggtca tgcgtatatt gcaaagctcc agcaataagt tgccgccctg
    31081 tgccttcccg ctgacgagcc ggatgaactg ctatccggct aacccgcctt ccgcgcaatg
    31141 tcgccgccag tggattgctg ccgtgcgccg ccagcgactg ggccaccaga ttaccccgcg
    31201 ggcgacgaaa acctgcccat accgcctgac tgagttcttg agataatccg ccctcatcca
    31261 ccagccacaa cgccccggca atctcgtttt cgccagccgc ctgtaaaaaa tgttgccctg
    31321 gtgcatccat catccggcgt aaatccagcg gcgaggtccg gtagtgcgca ccagacaata
    31381 actgataaac ctttaacggc gtttctggct cgcttcgcca taacgtttgt tcaaatgcgg
    31441 aaatgacgat attgccttgt ggtgtatggg tgaagttttc atcgttaaaa accagtgcct
    31501 cactaaccat tttttccagc gggcatccct gtgcccagcg gatcggctgt tgcagttcaa
    31561 aacggtgtaa atgcggaaag ccagcgcaaa atttcagcaa aaaaccacgt ccggtacctt
    31621 cgtagccctg caccgtagtg gttaacaacg ttcgaggaaa acgcgatacc agttgatgca
    31681 acaacggcgc aggtatggct gcggcttcgt cgaccaccag ccagtcggct tgctcatcgc
    31741 tggctaacaa ggcatccggt gcaataaagc gaaacttctc gcccgcaaat tgtgccagta
    31801 catccgttgc cgcttttgcg ggcgcggtga caatcgcact gcccgcaata cgagaaatga
    31861 gttgccctgc caacgccgac ttaccgcgcc cacgcgcagc cgttaccgct gccacgcccg
    31921 gcggcatggt cagtagctgc tgtaagagtt gctgttgttc tggttgtggt gcgccggtcg
    31981 cggggtgcca gtcagtacgg ggagtaaaat gcgccaacga gaacggctgg ttttgccgcc
    32041 agaggatagc gtcgttatcc gccgtaagta cgcgtttgag atgctggaca aaatgcggcg
    32101 tcgcaatagg gtcagggcaa tcactccagc gcagcgagtc ggcatcaggt tggttttccc
    32161 actcttccca tacagggagt aacaaaacca gccagcttcc cgctttcaac gttccgctaa
    32221 gtgctgcaaa ggcagcggca tcaaagccgt ggcgggcgtc gaataccgca tgccggaact
    32281 cgcgcccaag taaagtttgt agcgccgagg gtgaacagtg gttttcagca tctagctgcg
    32341 gcgaaatcca cagccagtcg ccaggtaagg catcacgcaa cttaagagca tgatcaaaac
    32401 accaaccctc ttctccgctc aacaccagca agcggcagat cccttcacgt ttcatttgcg
    32461 ctgttaatgt gtaaagcgca gtcagttcag ccatccctgc cccggaaatt aaatgctttt
    32521 accaaaagta ttgcattgtg ccggatcgcc gctgtcgaaa ccacgtttaa accagctgca
    32581 gcgttgctga gaagtgccat gtgtgaaact gtctggtact actcgcccct gactttgctg
    32641 ttgtaaacgg tcatcgccga tggcctgcgc cgcattcagc gcctcttcca gatcgccagt
    32701 ttccagaacg ccttgctgct gcatactatg cccccagaca ccggcaaaac agtcggcctg
    32761 gagttccata cgcacagata agcggtttac ttccgcctgc gtcgcgtttt gttgcagttg
    32821 acgaactttc ggctcgatgc ctaacagttt ctgcacatga tgaccgactt catgggcgat
    32881 aacgtacccc tgggcaaaat cgccatccgc gccaagtttg tctttcatgt catcatagaa
    32941 ggagagatcg atataaactg tgccatccgc cgggcaatag aacggcccca taatggactg
    33001 gcccgccccg cagccggtgc gcgtcattcc acggtacatg accagcttcg gttgctgata
    33061 ggtcttaccc atcttttcga actgttgtcc ccaggtgtct tccgtggttg ccagaatcac
    33121 cgaggtgaat tttgcggctt cgtcttcatt agggctaatt gaccgcgttg attgttgttg
    33181 agaaaccggc tgcccggtca tcaacccggt taaatcaacg ccatagtagc ctgcaaccag
    33241 cacgactatc agtaaaatca gcccgccttt accgctcggc agacggaaac cgggaccgcc
    33301 cattgacgga ccgccagagc tgttgcgcct gtcttcaaca ttgtcacttt cacgtcgccc
    33361 ttgccaacgc atagatacct caacaatata ttcattatag aaatgatcgt aggtggttca
    33421 gaggaagatt accacaggaa agcgggatga aatggcggag gaatgagcgt tcaatgcata
    33481 tattccgaga gcggttgtct tgccgtgcca gctgcacggc aagatgatga acagaaaaat
    33541 cagtccagct gtacacccag gcggtgggcg acggcttcat aggcttcgat caggccaccg
    33601 agactctggc ggaaacggtc tttgtccatt ttctccagtg tttctttgtc ccacaggcgg
    33661 ctgccgtccg gggagaactc atcgcccagt accacttcac ctttgtacag accaaactcc
    33721 agcttgaagt cgaccagaat cagaccagca tcatcgaaca gttttttcag cacgtcgttc
    33781 gctttgtagg tcagctcttt catacgcgcc aggttctctt tgctcaccca gccaaaggtt
    33841 tcgcagtaag attcgttgac catcggatcg tgcatggcat cgtttttcag gaacagatcg
    33901 aacagcggcg ggttcagctc aataccttct tcgattccaa gacgtttcac cagagagcca
    33961 gcagcacggt tacgcacgac acactcaacc ggcaccatat ccagcttttt caccagacat
    34021 tcggtatcgg agagcagacg ctccatttga gtcgggatac ccgcttcagc cagtttgctc
    34081 ataatgaagt agttgaactt gttgttcacc atacctttgc gatcaaactg ctcaatgcgc
    34141 gcgccatccc ctgctgacgt atcattgcgg aattcgagca ccaacaggtc cgggttttcc
    34201 gtgctgtata cggttttcgc tttaccacga tacaactcag cttgcttttg catctttatc
    34261 actcctgggt gtgaattaac gttttaaaat cttttgctgt ctggtgtgcc ggatgttttt
    34321 tcggatgcgg cgtgaacgcc ttatccgaac tacgtccgac aaacaaattt cgtgcgaatt
    34381 accgctaaaa tcgcgttttc ctgccgacgc acacgtttgc gtatcatatc agaaaaaagg
    34441 gccggatgat tccagccctg tatttttact tgctaaacgc agcctggaag acagctacca
    34501 gcgcgtcgtt ctgactctga gtcagagtat gacctttcgg atcgatgaac tgtaggctgc
    34561 tgcggttatc taaatcgcca acctgcagtt tatagtcacc ggatgccagg cctggatcgc
    34621 tcgcgcccag ttcctgccag tcgctgtcag acagcggctt ataagttacg gccatgttgc
    34681 cctgcgaacg ggtgctgtcg gtcactttca tgcccacttt ttccagcgac gctggcagac
    34741 gttgccaaac cacattgaac ggcccgcgta cgaccagcat tggtaaaccg gtgtcatcag
    34801 ctgcactttg tacgtccata gtggtggagg cacgattttg cgcagcgttc gcggcgtcag
    34861 tggcagattt atccagaccg gcggaaataa cgttcatcat ctccgtgctg taacgctgca
    34921 tggaagccgc gtctgcaacc ggtttgcccg cctgttccag gttcagcagt ttaaccgtaa
    34981 ccgcctgctg ataaccctgc ggcttaacag agatttgata acgaccacga tactgctcgt
    35041 cttcgtccag acggttccat tgtacccaat cggtggtcag tgtctgacca gcatcatcac
    35101 gttgggtgat ggtgtagttt ttcgcctgca gcacgctaac cacctgcggc cacagagtat
    35161 tgccacgacc attttccacc agcaatgaag cggtatcgcc cgtgaactgg gtacgcgcgc
    35221 cagaaaccag tgccagcggc tgggctggtg gacgaatgtc cagcgcctta ccgacagcac
    35281 cactaccgtt ggtcaccggg attgcataat caccggaggt caccggcaaa atcattccag
    35341 ccggggcatg aagctccgca agcggtgccg cttccaggta ggcttcatca ccactgacct
    35401 gacgcttata gcgtgagtca gaactacagg cagcgagtaa taaaacaagc gaaacacccg
    35461 caacctttgc caggcgcgac ttttgaacag agtaagccat caaatctccc taaactttac
    35521 agcaaaccgg catgcttaag cgccgctctg accgtctcac gaccactgtc ggtgattggt
    35581 gtcattggca ggcgcagcgt atcggtcgcc acaagaccca gttccttaca tgcccatttc
    35641 accgggattg gattgggttc gacaaatagt ttgttgtgta atggcatcag acgctgatta
    35701 ataacgcgtg cctcggcaaa atgcccttct gctgccagtt tgcacatctg ggccatatca
    35761 cgcgctgcga cgttagccgt aacggaaata accccatgac cgcccaattg catgaagtcc
    35821 agcgcgctcg catcatcgcc gctcagcaga acaaaatcat ctgaaaccag ctctttgatc
    35881 tggtttacac gcgttaagtt ccctgttgcc tctttgattc cgataatatt ttttactttc
    35941 gccagacggc ccaccgtttc cgggagcaga tcgcagccag tacgggacgg cacattatac
    36001 agaatttgcg gcaggtcagt atgctcagcg atggctttga aatgctgata caaaccttct
    36061 tgcgacggac gattgtagta aggggttacc gtcaggcagc cgacgatacc actgtcattg
    36121 aagcgctgcg tcaggctaat ggcttccgca gtagcgttag cgccggtccc ggcaattacc
    36181 ggaatgcgcc catcagccag atccagcgtc atcatcacca catcagcatg ttcgtcatga
    36241 tttaaggtag cggactcgcc agtggtgcca acagaaacga tcgccgaagt accgctggcg
    36301 acatgataat caatcagttt tttcaagcta gcccgacaga cattaccttt ttcatccatc
    36361 ggagtaacaa tcgcgacaat acttcccgtg aacatgggcc atcctctgtg caaacaagtg
    36421 tctcaatggt acgtttggta tggcattaaa agcaagcaga cagaaccgtt ctgattgttg
    36481 tatgcatgat ttttttatgc tttccttaag aacaactcac cccttaaagg aataaccagt
    36541 ttgacactgt catcgcaaca ttatctggtg atcactgcgt tgggtgccga tcgccctgga
    36601 attgtgaaca ccatcacccg tcatgtcagt agttgcggct gtaatattga agacagtcgc
    36661 ctggcgatgc tgggagaaga gttcacgttt atcatgctgc tttccggttc atggaatgcc
    36721 atcactctga ttgaatcaac gttaccgttg aaaggtgccg aactggatct tttaatcgtg
    36781 atgaagcgca cgacggcgcg tccgcgtccg ccaatgccag catctgtctg ggttcaggtc
    36841 gatgttgcag actccccgca tttaattgaa cgcttcacag cacttttcga cgcgcatcat
    36901 atgaacattg cggagctggt gtcgcgcaca caacctgctg aaaatgaacg ggctgcgcag
    36961 ttgcatattc agataaccgc ccacagcccc gcatctgcgg acgcagcaaa tattgagcaa
    37021 gcgttcaaag ccctatgtac agaactcaat gcacaaggca gtattaacgt cgtcaattat
    37081 tcccaacatg atgaacagga tggagttaag taatgaatcc actgaaagcc ggtgatatcg
    37141 caccgaaatt tagcttgccg gatcaagacg gagaacaagt taatttgacc gacttccagg
    37201 gacagcgtgt tctggtttat ttctacccga aagccatgac ccccggctgt accgtacagg
    37261 cctgcggctt acgcgataac atggatgagt tgaaaaaagc gggcgttgat gtgctgggta
    37321 tcagcaccga taaacccgaa aaactctccc gttttgcgga aaaagagctg cttaacttta
    37381 cgctcctgtc tgatgaggac caccaggtgt gcgaacaatt cggcgtctgg ggtgaaaagt
    37441 ccttcatggg caaaacctac gatggtattc atcgcatcag cttcctgatt gacgctgatg
    37501 gcaaaatcga acatgtcttt gacgacttca aaaccagcaa tcaccacgac gttgtgctga
    37561 actggctgaa agaacacgcc tgattacttt gctccattcc gtgctggctg cgcttgcggc
    37621 cagcatacct cacttctcgt gatcaagatc acattctcgc tttcccctgc gacacgggtg
    37681 tcgaatccat tttttgctga acgttaatga ccatcatttt tgtaccgttc agaatccagt
    37741 taatacataa cttattgaat agattgagtt aatcagaatg gcacccttta tgcaatatga
    37801 aatgcaatgt ttcatatcat tttcaaggag ccgacatgaa ccgctttgtg gtggccgaac
    37861 cactgtggtg tacaggatgt aatacctgtc tcgctgcctg ttcggacgtg cataaaacgc
    37921 aaggtttaca gcaacacccg cgcctggccc tggcgaagac gtcaacaatc actgcccctg
    37981 tcgtgtgtca tcactgtgag gaagcccctt gcctgcaggt ctgcccggtc aatgccatct
    38041 ctcagaggga tgatgcgatc caactcaacg aaagcctctg tattggctgc aagctttgcg
    38101 ccgtggtctg cccatttggc gcaatcagcg cttcaggaag ccgtccggtg aatgcccatg
    38161 cgcaatatgt ttttcaggct gaaggctcac tcaaagacgg cgaagaaaac gtgccgcccc
    38221 aacatgcgtt gctgcgctgg gaaccgggtg tccagaccgt cgcggtgaaa tgcgacctgt
    38281 gtgatttctt gccagaaggt ccggcctgcg ttcgcgcttg cccgaatcag gcgttacggc
    38341 tgatcaccga cgatagcctg caacgtcaga tgaaagaaaa acagcgcctt gccgcaagct
    38401 ggtttgccaa tggcggggag gatccccttt ccctcactca ggagcaacac taatggatgc
    38461 cctgcaatta ttaacctggt cgctgatcct ctatctgttt gccagtctgg cttcgctgtt
    38521 tttactcggt ctggacagac tggctattaa gctttccggc atcacatcgc tggtgggcgg
    38581 cgtgattggc atcatcagcg gaattacgca attacatgct ggtgtaactt tagtcgccca
    38641 ttttgccacc ccttttgact ttgccgattt aaccctgcga atggatagcc tctcggcatt
    38701 tatggtgctg gttatctcct tgctggtggt ggtttgttcg ctctattcat tgacttatat
    38761 gcgcgaatac gagggcaaag gcgcggcggc gatgggcttc tttatgaatc ttttcatcgc
    38821 atcgatggtt gccctgctgg tgatggacaa cgctttttgg ttcatcgtgc tgtttgaaat
    38881 gatgtcgctg tcttcctggt ttctggtcat tgccaggcag gataaaacgt cgatcaacgc
    38941 tggcatgctc tactttttta tcgcccacgc cggatcggtg ctgattatga tcgccttctt
    39001 gctgatgggg cgcgaaagcg gcagcctcga ttttgccagt ttcgcacgct ttcactttct
    39061 ccggggctgg cgtcggcggt gtttctgctg gcctttttcg gttttggcgc gaaagccggg
    39121 atgatgccgt tgcacagctg gttgccgcgc gctcaccctg ccgcaccatc gcacgcttcg
    39181 gcgttgatgt ctggcgtaat ggtcaaaatc ggtattttcg gcatcctgaa agtagcgatg
    39241 gatctgctgg cgcaaacggg tttgcctctg tggtggggca ttctggtgat ggcgatcggc
    39301 gcaatctccg cgctcctggg cgtgctatat gcgctggcgg aacaggatat caaacggctg
    39361 ctggcctgga gtaccgtcga aaacgtcggc attattttgc tggcagtcgg tgtggcgatg
    39421 gacggtctgt cactgcacga cccgctgctc accgtggttg gactgctcgg cgcactgttt
    39481 catctgctca accatgcgct gttcaaaggg ctgctatttc tcggcgcgga cgcgattatt
    39541 tcgcgtttgc atacccacga catggaaaaa atgggggcac tggcgaaacg gatgccgtgg
    39601 acagccgcag catgcctgat tggttgcctc gcgatatcag ccattcctcc gctgaatggt
    39661 tttatcagcg aatggtacac ctggcagtcg ctgttctcac taagtcgtgt ggaagccgta
    39721 gcgctacaac ttgcgggtcc tattgctatg gtaatgctgg cagtcactgg tgggctggca
    39781 gtaatgtgct tcgtcaaaat gtacggtatt actttctgtg gtgcgccgcg cagtacacac
    39841 gctgaagagg cacaggaagt gccaaatacg atgatcgtcg ccatgctact gctcgcggca
    39901 ctctgcgtat taattgcgct tagtgccagt tggctggcac cgaagataat gcatattgcc
    39961 catgcgttta ccaatacccc tcccgtcact gtcgccagcg gaatagcact tgtacccggc
    40021 acgtttcata cacgggtcac tccttcatta ctgttgctgt tactactggc gatgcctttg
    40081 ctgccaggcc tttactggct gtggtgtcgt tcgcgccgcg cagcgtttcg tcgcacagga
    40141 gatgcctagg catgcggcta cggctgggaa aacgcgatgg ctccgtcagg caatggcgtg
    40201 atgcagccgc tgcgtgtggt cttctctgcg ttatttcgtc tacgacaaca gctcgaccct
    40261 acgctgaggc taaataaagg tcttgcgcac gtcaccgcca gggctcagag cacagaaccc
    40321 ttctgggatg agcgggtgat ccgccccatc gtgagcgcca cccaacggct ggtcaaagaa
    40381 atacagcatc tgcaaagcgg cgactttcgt ctctattgcc tgtatgtggt cgccgcactg
    40441 gttgtgctgc taatcgctat tgccgtctaa ggaaatcacc atgagacaaa ctctttgcga
    40501 cggatatctg gtcatttttg cgttagcaca ggccgtgatt ctgctgatgc taaccccact
    40561 ttttacgggt atttcccggc agatacgcgc gcgtatgcac tcccgccgcg ggccggggat
    40621 ctggcaggat tatcgcgata tccacaaact gtttaaacgc caggaagttg cgccgacatc
    40681 ttcaggtctg atgttccgcc tgatgccgtg ggtattaatc agcagcatgc tggtgctggc
    40741 gatggcctta ccactattta ttaccgtttc cccttttgcg ggcggcggcg atctgctcac
    40801 ccttatctat cttcttgccc tgtttcgttt tttctttgct ctttccgggc tggataccgg
    40861 aagtccgttt gcgggagtcg gtgccagtcg cgagttgacg ctcggcattc tggtcgaacc
    40921 aatgcttatt ctctcactgc tggtattggc gctgatagca ggttccacgc atatcgagat
    40981 gatcagcaat acgctggcga tgggctggaa ctcgccgcta accaccgtac tggcgttact
    41041 ggcctgtggt tttgcctgct tcattgagat gggaaaaatt ccctttgatg ttgctgaagc
    41101 agaacaggaa ttacaggaag gcccgctgac cgaatattcc ggtgccgggc tggcgctagc
    41161 gaaatggggg caggggctga aacaggtcgt gatggcagca ctgtttgtgg ccctgtttct
    41221 gccctttggg cgcgcgcaag aactttctct cgcctgcctg ctgacttcac ttgtcgttac
    41281 gctgctcaag gttttgctga tttttgtact ggcctcaatc gcagaaaaca cgctggcacg
    41341 cgggcgtttt ttactcattc accatgtgac ctggcttggc ttcagccttg ctgcgcttgc
    41401 atgggtcttc tggttaaccg gtctgtaagg agcactgacg gaatatggaa aatcttgctc
    41461 tgacgacgtt aatgctgcct tttatcggcg cactggtcgt ttcgttttcg ccacaacgtc
    41521 gggccgccga atggggggtt ttgttcgccg cgctgaccac gctgtgcatg ttgtcgctga
    41581 tctccgcgtt ttatcaggcc gataaagttg ccgtcacgtt gacgttggtc aacgtggggg
    41641 atgtggcatt gtttggcctg gtcattgatc gcgtgagtac gctgattctg tttgtggtgg
    41701 tgtttctcgg tttgctggtc acgatctact ccacgggtta tctgacggat aaaaatcgcg
    41761 aacacccgca taacggcacg aatcgttatt acgcattttt gctggtgttt atcggcgcga
    41821 tggcgggact ggtactctcc tcgacgctgc tcggtcagtt gttgtttttt gaaattacgg
    41881 gcggctgctc ctgggcgttg atcagttatt accagagcga taaagcgcag cgttcagcac
    41941 taaaagcgtt gcttatcacc catatcggtt cgttggggtt gtatcttgcc gccgccacgc
    42001 tgtttttgca gaccggaacg tttgtgctta gcgcgatgag cgagttacat ggcgacgcac
    42061 gttatctggt ttatggcggc atcctgtttg ccgcgtgggg gaaatcggcc cagctaccga
    42121 tgcaagcgtg gctaccggac gcaatggaag cgccaacacc gatcagcgac tatctccacg
    42181 ccgcatcgat ggtgaaagtg ggcgtttaca tttttgcccg cgccattatc gacggcggca
    42241 atatcccgca tgtgattggc ggcgttggca tggtcatggc actggtcacc attctttatg
    42301 gctttctgat gtatttgcca cagcaggata tgaagcggtt gctggcctgg tcgaccatca
    42361 ctcaacttgg ctggatgttc ttcggcttgt cgctctccat cttcggctcg cggctggcgc
    42421 tggagggtag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg tttttccttg
    42481 tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg cgtggcgtat
    42541 tgcacaccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggca attaccggtg
    42601 tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt tttgcgttgt
    42661 cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa tcggtcgcca
    42721 gtttcgcctg gtttattcgc tggtttggtc gcgtcgtgcc gggcaaaccg agcgaggccg
    42781 tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta ctgattgtga
    42841 tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat gatgaatgac
    42901 cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc tgtttgtgat
    42961 tagcgtcaaa agctatcgcc tgtcatgcgg attttaggcc tgccagtcgc tggtgctggt
    43021 gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga tctggtccgc
    43081 cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg ctgcacgaaa
    43141 tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg cactgctcgc
    43201 ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac cgatggctac
    43261 cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc tgctgtgcat
    43321 tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg aaaacggctc
    43381 ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa taggtatcgc
    43441 taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa tatggcgtac
    43501 ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag atgagttatt
    43561 ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc tgttttgcct
    43621 gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc ttagggatca
    43681 cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca ggagaaatat
    43741 tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc gccattcttg
    43801 gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat gaagtggcac
    43861 acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat ctgtttttgt
    43921 tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg gcgatcgaag
    43981 ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca tcgctggaat
    44041 cagcatggaa gtacatcatt atttgtactg ttggtgtcgc ttttggtctg ttcggtaccg
    44101 tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg atattctgga
    44161 gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg gcctttgtgt
    44221 ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc tggctgccgg
    44281 atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta ttgctgaact
    44341 gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaggccatc ggcagcgatt
    44401 tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg gcatttttca
    44461 ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag aacatggggc
    44521 tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg ctgctgcaca
    44581 ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat gtactgctca
    44641 agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg ccatttaccg
    44701 ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc aacatttttc
    44761 ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg attatcgtcc
    44821 tgctgttatt gctgttaacg ctggtgctcg cgggcctggt acggatggct gcgcgggtgt
    44881 taatggcgaa accgccgcag gccgttaacc ggggtgatct cggctggttg accacctcgc
    44941 caatggtgat tctgctagtc atgatgctgg cgatgggaac gcatattcca caacctgtca
    45001 tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac ctgcctgcac
    45061 aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg gagaaacaca
    45121 gtgaacgtta attcatcgtc aaatcgtggc gaagcgattc tcgccgccct gaaaacgcag
    45181 ttccccggcg cggtgctgga tgaagagcga caaacgcctg aacaggtcac cattacggtg
    45241 aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga tggctggctt
    45301 ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt ttattatgcc
    45361 ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt cgatgccgac
    45421 agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg cgagcgagaa
    45481 attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg cctggtgttg
    45541 cccgatgact ggccggaaga tatgcatccg ctgcgcaaag atgcgatgga ttatcgactg
    45601 cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg caacagcgat
    45661 gcgcgggtga tccctgtcgg cccgctgcat atcacctcct atgaaccggg tcacttccgc
    45721 ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta tgtccatcgc
    45781 ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt cttatcggac
    45841 cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc ggttgaaaat
    45901 gcactgggga ttgaggtgcc gcaacgagca catactattc gctcgattct gctggaagtc
    45961 gagcggctac acagtcattt gctcaacctt ggcctctcct gccattttgt tggttttgat
    46021 accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc ggaattgctg
    46081 accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg cgatattctc
    46141 aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgcgccga cgtgtcggag
    46201 ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg cattggcatt
    46261 ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg cgcagtggtt
    46321 ttgcccgtga tttgcgcttt gatcacccct acgccgacta cggcaatatt ccaaaaacac
    46381 tgtttacctt taccggcggc gatgttttct cccgcgtgat ggtccgtgtc aaagagacgt
    46441 ttgattcgct ggcaatgctg gaatttgccc tcgacaacat gccggatacc ccactgctga
    46501 ccgaaggctt tagctataaa cctcacgcat tcgcgctggg ctttgttgaa gcgccacgcg
    46561 gtgaagacgt gcactggagc atgctcggtg ataaccaaaa attgttccgc tggcgctgtc
    46621 gtgccgccac ctacgccaac tggccggtgt tgcgttacat gctgcgcggc aataccgttt
    46681 ctgacgcacc gctgattatc ggtagccttg atccctgcta ctcctgtacc gaccgtgtga
    46741 cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgct gtataaagag atcgaacgct
    46801 acggcattga tcgtaaccgt tcgccgctga agtaaggaca gaagatgctg aagttactga
    46861 aaactattat gcgcgccgga accccgacgg tgaaatatcc cttcgcgcca ctggaggtca
    46921 gcccaggctt tcgcgggaaa ccggacctga tgcccagcca atgtattgcc tgcggtgcct
    46981 gcgcctgtgc ttgtccggca aatgcgctga ctatccagac cgacgaccag caaaattcgc
    47041 gcacctggca gctctatctg gggcgttgta tttactgcgg acgttgtgaa gaagtgtgcc
    47101 cgaccagagc catccagctt accaataact ttgaactgac cgtcacccat aaagccgatc
    47161 tctataccca cgcgacgttc catctacaac gttgcagccg ttgcgaacgc ccgtttgccc
    47221 cgcaaaaaac cgtcgcactg gctgctgaat tgttggcaca gcaacaaaat gctccacaaa
    47281 accgcgaaat gttatgggcg caagcgagcg tctgcccgga gtgcaaacaa cgcgcgacgc
    47341 tgatcaacga cgatacagat gtaccgctgg tggctaagga gcagctatga gtccagtgct
    47401 tacgcaatat gtcagccagc ccatcacgct ggacgagcaa acgcaaaaga tgaagcggca
    47461 tttgctacag gatatccgtc gctcggctta cgtttatcgc atcgattgcg gcggctgcaa
    47521 cgcctgtgaa atcgaaattt ttgctgccat cacccccgta ttcgacgcag aacgttttgg
    47581 cattaaggtt gtttcatcac cgcgtcacgc cgatattttg ttatttactg gcgcagtcac
    47641 ccgggcgatg cgtatgcctg cacttcgggc gtatgagtct gcccccgatc ataaaatctg
    47701 tgtttcctac ggcgcgtgcg gtgtcggcgg cggtattttc cacgatctct acagcgtctg
    47761 gggcggcagc gacaccattg tccccattga tgtttggatc cccggctgcc cgccaacacc
    47821 ggccgccacc attcacggtt tcgccgtggc gctcggtttg ctgcaacaga agattcacgc
    47881 ggtggattat cgcgatccca ccggggtgac tatgcaaccg ttgtggccgc agatcccgcc
    47941 atcacagcgt atcgccattg agcgagaagc gcggcggctg gcgggctatc gtcaggggcg
    48001 agaaatttgc gatcggctcc tgcgccattt aagcgacgat cctacaggaa atcgggttaa
    48061 cacctggttg cgcgatgccg acgatccacg tctcaatagt atcgttcagc aactctttcg
    48121 cgtactccgg gggttacatg actgaagagt gcggggaaat tgttttctgg acgctgcgaa
    48181 aaaagtttgt cgccagtagc gacgagatgc cggaacacag ctctcaggta atgtattact
    48241 cgctggctat cggtcatcac gttggcgtga ttgattgtct gaatgtcgcc ttccgctgcc
    48301 cactgacgga atacgaagat tggcttgcac tggtcgaaga ggagcaagcc cgacgtaaga
    48361 tgctgggggt gatgactttt ggtgagattg ttattgacgc cagccacacc gccctgttga
    48421 cccgggcatt cgcgccactg gcagatgacg cgacgtctgt gtggcaagcg cgtagcattc
    48481 aattcattca tctgttggat gaaattgtgc tggaaccggc tatctatctg atggccagaa
    48541 aaattgcgtg agaaggattt ctcattaata aggactgttg atggctatgt cagacgaagc
    48601 gatgtttgcc ccgccgcaag gaataacaat tgaagcggta aacggaatgc tcgcggagcg
    48661 gttagcgcag aaacacggca aggcgtcttt attacgcgcc ttcatcccgc tgccgccgcc
    48721 gttcagcccg gtacaactta ttgaactgca tgttctcaaa agcaacttct attaccgcta
    48781 ccatgatgat ggcagcgatg tgacggcaac aacagagtat cagggcgaga tggtcgatta
    48841 ttcgcgtcac gccgtccttc tcggcagtag ttgaatggcg gagctacgct ttattcgtac
    48901 ccacggcagc cgttttactc cccaggattg cacactgttt aactggctgg cacgcattat
    48961 caccccggtt ctgcaatcat ggctcaatga tgaagaacag caggtggcgc tgcgtttgct
    49021 ggagaaagat cgcgatcatc atcgggtact ggtggatatc actaatgcag tgctgtcaca
    49081 tcttgatctc gacgatctga tcgctgacgt cgctcgtgag atccatcatt ttttcggtct
    49141 ggcttcagtc agtatggtac tgggcgatca tcgaaagaac gagaagttca gcctgtggtg
    49201 cagcgatctt tctgcctcac attgtgcgtg tctgccacac aatatgcctg gcgacagtgt
    49261 attgctgaca caaacgctac aaacccgaca accgaccttg acgcaccgtg cagatgatct
    49321 gtttctctgg caacgcgacc cgttattact cttacttgca tctaacggct gcgaatctgc
    49381 gctccttata ccgcttacct ttggcaacca tacaccgggt gcattgttgc tggcgcatac
    49441 ctcttccact ctctttagtg aggaaaactg ccagctacta caacacatag ccgatcgcat
    49501 cgctattgcc gttggcaatg ccgatgcctg ccgtagaatg accgatttgc aggaaagttt
    49561 gcagcaagaa aaccaccagc ttagcgagca gctcctttcg aatctgggcg tcggtgacat
    49621 tatctatcaa agccaggcaa tggaagacct gctccagcag gtagatattg tggcgaagag
    49681 cgacagtacg gtgttgattt gcggtgaaac cggaaccggc aaagaggtga tcgccagagc
    49741 gatccatcaa cttagcccgc gacacgacaa gccgctggtc aaaatcaact gcgctgccat
    49801 ccccgccagt cttctggaaa gtgagttatt cggtcatgac aaaggggcgt ttactggtgc
    49861 gattaatacc catcgtggtc gttttgaaat tgccgatggc ggcacgttgt ttctcgatga
    49921 aattggcgat ctgccgttag aacttcagcc taaactgctg cgcgtattgc aggaacggga
    49981 gattgagcgt ctcggcggga gtagaacgat cccggtaaat gtcagagtca ttgccgccac
    50041 caaccgtgat ttgtggcaaa tggttgaaga tcgccagttt cgcagcgatc tcttttatcg
    50101 cctgaatgtc ttcccactgg aattgcctcc gctgcgcgac cgtccggaag atatccctct
    50161 tttagcaaag catttcacgc aaaaaatggc gcgccatatg aatcgttcaa ttgacgccat
    50221 cccgaccgag gcgctacgcc agttgatgtc gtgggactgg ccgggcaacg tgcgcgagct
    50281 ggaaaacgtg attgagcgag cggtactgct gactcgcggt aacagtctga atttacatct
    50341 taatgtccga caaagccgtt tactgccgac gctaaatgaa gattcagcgc ttcgcagttc
    50401 aatggcgcag ttgctgcacc cgacgacgcc agagaatgac gaagaagaac gtcagcgcat
    50461 tgttcaggta ttgcgagaaa ccaatggcat tgttgccggg ccccgtggcg cggcgacacg
    50521 attagggatg aagcgcacca cgctgctgtc acgaatgcag cgtctgggaa tctcggttcg
    50581 cgaggtgttg taatctgctt ttgcaggagt atgcatgaga aacaaactct ctttcgactt
    50641 gcagttgagc gccagaaaag cggcaatcgc tgaacggatt gccgcccata aaattgcccg
    50701 cagtaaagtg tcggtctttt taatggcgat gtccgctggc gtgtttatgg cgatcggatt
    50761 tactttttac ctttccgtta tcgccgatgc cccgtcttca taggcattaa cccatctggt
    50821 gggcggcctt tgctttacac ttggctttat tttgctggcg gtttgcggca ccagcctgtt
    50881 cacctcgtcg gtaatgacgg taatggcaaa aagtcggggc gttattagtt ggcgaacttg
    50941 gctgattaac gcacttctgg tggcctgcgg taatctggca ggtattgcct gtttcagttt
    51001 gttaatctgg ttttccgggc tggtgatgag tgaaaacgcg atgtggggag tcgcgatttt
    51061 acactgcgcc gagggcaaaa tgcatcatac atttactgaa tctgtcagcc tcggcattat
    51121 gtgcaatctg atggtttgcc tggcgctgtg gatgagttat tgcggacgtt cgttatgcga
    51181 caaaatcgtc gccatgattt tgtccatcac cctgtttgtc gccagtggct ttgagcactg
    51241 tatcgccaat ttgtttgtga ttccgttcgc cattgccatt cgccatttcg cccctacctc
    51301 cttctggcaa ctggcgcaca gtagcgcaga caattttccg gcactgacgg tcagccattt
    51361 tattaccgcc aatctgctcc cggtgatgct gggtaatatt atcggcggtg cagtgctggt
    51421 gagtatttgt tatcgggcta tttatttacg tcaggaatcc tgaaaaatca gcccggcgaa
    51481 acagttcgtc gggctgaaga ttattcttgc gcgatttgcc catcgggcca ggcgtggatc
    51541 acggctttga tcagcgtcgc caatggaatg gcgaagaata cgccccagaa tccccacaaa
    51601 ccaccgaaga tcaccaccga taaaataatc accagcggat gcaggttaac cgcttcggag
    51661 aacaacaccg gtactagcag gttgccgtcc agcgcctgaa taatcagata caccgcgaag
    51721 cagctccaga attcagtacc tgcgccaaac tggaatagcg ccacgccaac caccggaatg
    51781 gtcaccacaa atgcgccaat gtacggaata agaaccgaga agccgaccag cactgccagc
    51841 agcagcgaat agttcagccc gaagagcaag aaccccagcc aggtggcgat ccccaccacg
    51901 atcatctcca gcactttgcc gcggatatag ttggtgattt gttgattcat ctccttccac
    51961 acctgtcctg ccagcccacg gttacgcggc agcacccggc gaacggcgtt cagcatctgc
    52021 tctttgtctt tcagcaggaa gaagaccatc aatggcacca gcaccagata gacggctatg
    52081 gtcagcaaac cgaccagcga ggcgagggaa attttcacca ccgaatcgcc catggtcagc
    52141 atccgactgc gcatattttc ggccattgca tcaataatgc ccgcgtccat taacaccgga
    52201 tagcggcgcg gcaacgtggc ggcaaagtca gaaagcttat tgagcatccc cggcatatcg
    52261 cggattaagt agatgccctg ttgccaggcg ataggcagta ccacgaacgc catcagtagc
    52321 aatataccga caaaaaccac caatacaatc gacgtcgccc agcggcggga gcagccaata
    52381 gattgcaggc gcacggttgg ccattccagc aaataagcca gcacaatagc caccagcaac
    52441 ggagcaagca ggccactaaa gaaaaagata atgccaaatc cggcaactaa aataaccagc
    52501 aaggcaatcg cttccgggtc gctaaaacgg cggcgatacc attgcatcaa catttcgagc
    52561 ataaagacct tccctgaacc tcaagagcgg gattgcgatc cgcaattgta tcgaaatgtc
    52621 acaaaaaaga cttcgctttt tatgacggat tcaggaaact gaaaagtcat ttgagtgggc
    52681 taatcttcgc cgttacactc aaaggcggcg aggtgggaac gatatttcac agtatcggtc
    52741 aaatgactat cttcagaaat acaggataga ggttatgttc aggcagttga aaaaaaacct
    52801 ggttgcaacc ctcattgctg ctatgaccat tggtcaggta gccccggcgt ttgccgacag
    52861 cgcagacacc ttgccggata tgggaacctc cgcaggaagc acgctttcca ttggtcagga
    52921 aatgcagatg ggcgactatt atgtccgcca gctacgcggc agcgcgccgt taattaatga
    52981 cccgctgtta acgcaatata ttaattcgct ggggatgcgt ctggtttcgc atgccaattc
    53041 ggttaagaca ccgtttcatt tttttctgat caacaacgac gaaattaacg cctttgcttt
    53101 ctttggcggc aacgtggtgc tgcactctgc cctgttccgt tattccgata acgaaagtca
    53161 actggcttca gttatggcgc acgaaatctc ccacgtcacc caacgtcacc tggcgcgagc
    53221 gatggaagat caacaacgca gcgcgccgct gacctgggtc ggcgcgttag gttctatttt
    53281 actggcgatg gccagtccgc aggcggggat ggcggcgctg accggtacac tggcgggaac
    53341 gcgccagggg atgatcagtt tcacccagca aaatgaacag gaagcggacc gcattggtat
    53401 tcaggtgctg caacgctcgg gattcgatcc acaggcgatg ccgaccttcc tggaaaaatt
    53461 gctcgatcag gcgcgttact cctcgcgccc gccggaaatt ttattgactc acccgttgcc
    53521 ggaaagtcgt ctggcagatg cccgcaaccg tgctaatcag atgcgcccga tggtggtgca
    53581 gtcgtcggaa gatttctatc tggcgaaagc gcgcacactg gggatgtata attccggacg
    53641 taaccagctc accagtgatt tgctggatga atgggcgaaa ggaaacgttc ttcagcaacg
    53701 agcggcgcaa tatggtcgtg ctttacaggc gatggaagcc aataaatacg acgaggcgcg
    53761 taaaacgctg caaccgttac tggcggcaga acctggcaac gcatggtatc tcgatctggc
    53821 taccgatatc gatcttgagc aaaacaaagc caatgaggca atcaatcgcc tgaaaaatgc
    53881 ccgcgattta cgcaccaatc cggtgttgca gctcaacctg gcgaacgctt atctgcaagg
    53941 cggtcaacca caagaagcgg ccaatattct gaatcgctac acctttaata ataaagatga
    54001 cagcaacggc tgggatttgc tggcacaggc ggaagccgcg ctaaataacc gcgatcagga
    54061 gctggctgcg cgagcagaag gttatgcgct cgccggacga ctcgatcagg ccatttcgct
    54121 gttgagtagc gccagttcgc aggtgaaatt aggcagcctg caacaagcgc gttacgatgc
    54181 gcgcatcgac cagttgcgcc agctgcagga acgctttaag ccttatacca agatgtaatt
    54241 cgcattaaag gaagaacaat gaccaaacag gtaaaaatct accataaccc acgctgttca
    54301 aagagccggg aaacgctgaa cctgctgaaa gaaaacggcg tggagccaga agtggtgctc
    54361 taccttgaga cacccgccga tgcggcaacg ctgcgcgatt tgctgaaaat gctggggatg
    54421 aatagcgccc gtgaattgat gcgccagaaa gaggatcttt ataaagaact aaacctggct
    54481 gacagttcgc ttagcgaaga ggcgctgatt caggcaatgg tggataatcc gaagctgatg
    54541 gagcgcccga ttatggtcgc gaatggcaaa gcgcggattg gtcgtccacc ggagcaggta
    54601 ctggaaatcg tgggttaaga tgtgctccat cgctagttga agcacattgc cggatgcgac
    54661 gcttgccacg tcttatccgg cctacgaata ctatggatta acttcgcgta ggtcaggcgt
    54721 tcgcgccgca tccgacaata aacaccttat ctacaacttc agaatttctt tcacaaacgg
    54781 aatggtcagc ttacgttgcg cggtaatcga cgcacgatcc aactgatcca acgtcataaa
    54841 tagcgtgcgc atttctctgt cgagccgctt cagcaagaaa cgccccacat cttccggcag
    54901 ttcaaaacca cgcaaacgcg cgcgtaactg tagcgcctgc aacttatctt catcagaaag
    54961 tggctgcaat ttgtagatct gcccccagtc gagacgcgac gcgagatccg gtaatcccag
    55021 attcaactgc cgcggtggac gatcgccggt gatcaacaac cgtgttttgc ccgattccag
    55081 aattcgattg tagagatcga aaatcgccat ctcccacaac tcatcgcctg caatacactc
    55141 aatgttgtcg atacagacca gcgacaaatg ctccataccg tcgagcactt ccggaacaaa
    55201 ccaggtgcgt ttatccagcg ggacatagcc caccgcatcg ccacgctgcg acaattccgc
    55261 gcaagccgcg tgcagcagat ggctgcgccc cgcgccttcg cgtgcccaga gatagatgta
    55321 accgctatgt tcctgacgca gcacgttttg cagcgcggcc agtaaagagg agttatcccc
    55381 cggccagaaa cttgcaaagg tttcgtcgtc aggaagataa agtggcaaag agagctgtgc
    55441 cggtgtgttc agagatacct caaccaggat ttcacaaaat cgcgagaagt ttaccacaga
    55501 atcccatgat gtttgaaccg ggcagcaaca ctgcccggtc ggtacattat ttgtctgtta
    55561 tatccgcgtc ttctgcgtcc agcaccactt cttcagggcg cagcacactg atcagtttga
    55621 aaatcaaact taagccgata ccgacgatgg tcgccagcgc catacctttt aactccgccg
    55681 cgccgatgtt taccttcgcg ccactgacgc cgatgatcaa aatcacggaa gtcaggatca
    55741 ggttctgtgc tttgttgtaa tccactttcg attcgatcaa aacacgaata ccggaagcac
    55801 caatgacacc ataaagcagc agcgaaacgc cgcccatcac cggcaatggg atcatctgga
    55861 tagcggcagc cagtttaccg acgcaggaaa gcaggatagc gaaaatcgcc gccccgccga
    55921 taacccaggt actgtaaaca cgggtgatcg ccatcacgcc aatgttttct ccgtaagtgg
    55981 tatttggcgt agagccaaag aagccggaga tcacggtcga caggccgtta gcaaacatcg
    56041 aacggtgcag acctggatcg cgcagcagat cttttttgac gatattagcc gttactacca
    56101 ggtgccctac gtgttcggca ataaccacta acgccgctgg cagaatagtc agaatggcaa
    56161 accactcgaa gcgcggcgta tagagggttg gcagtgcaaa ccagtgagca ttaataatcg
    56221 gcgtggtatc gacaattccc attgcgaaag agagcgcgta ccccaccagc acgccaatta
    56281 aaatcgggat aattgccagg aaaccacgaa acagcacgga acctaaaacc gtgaccgcca
    56341 gggtggtaat agagatgatg atggttttgg agtttggcgt ttgcccttca gccgggagta
    56401 aacccgccat accggcagct acgcccgcca gctccagacc gatgacggca acgattgcgc
    56461 ccattgccgc aggtggaaac agcacgtcca accagccggt ccccgctttc ttcacgataa
    56521 aagaaaccag gcagaacagc acgccgcaca taataaagcc gcccagcgcg acttcatacc
    56581 ctaacggcaa cagtaacaat accggtgaaa taaaggcaaa gctggaacca agataagccg
    56641 gaattttccc tttacagatg aagagataca gcagcgttcc aataccgtta aataacagta
    56701 cagtcgccgg gttaatatga aataagacgg gcaccaggac ggttgcacca aacatggcga
    56761 acaaatgttg caaactaagc gggattgtct gtaaaagtgg cggtctttca ctcaccccga
    56821 tagcacggcg cgtcatagta ttatcctctg tattatgtgt tataggcgct ttactcaaaa
    56881 aaaagccgac tcttaaagtc ggctttaatt atttttattc tttatttcgt accaaagatt
    56941 ttgtcaccgg catcgccgag gcccggaata atgtatccgt gctcgttcag tccctggtca
    57001 atcgatgcag tatacagttc gacgtccggg tgcgcttttt ccagcgcagc gataccttct
    57061 ggcgcagcga ccagcaccag aactttgatg ctgctgcagc ccgctttttt cagcaggtcg
    57121 atggtcgcga taacggaacc accggttgcc agcattgggt caacgatcag cgccatacgc
    57181 tcatcgatgt tagaaaccag tttctggaag tacggtaccg gctccagcgt ttcttcatta
    57241 cggtacatac cgacaacgct gatgcgcgcg ctcggaacgt tttccagcac accgtccatc
    57301 ataccaagac ccgcacgcag aattggcaca acggtaattt tcttaccttt gatctggtcg
    57361 atttctaccg ggccgttcca gccttcgata gttacttttt ccgtttcgag gtcggcggtc
    57421 gcttcgtaag tcagcaggct acccacttcg gaagcgagtt cgcgaaagcg cttggtgctg
    57481 atatcttgct cacgcatcag tcccagcttg tgtttgacga gtgggtgttt gacttccacg
    57541 atcttcatac tctttctcct ttgaggggca gccacaaaaa aaatcgacgg attatacctc
    57601 ctttcttcaa ggcggcaata ttattttcgt tgactttagt caaaatgata acagtttgag
    57661 ataaagttat tttatattca aatggttatg aaagaagatt attccatccg aaaactaacc
    57721 tttgccctgg cacaagtctt ctttcgccgc gcgcccgggg aaaagacgtg caaaaaggtt
    57781 gtgtaaaaca gtctcgcaaa cgtttgcttt ccctgttaga attgcgccga attttatttt
    57841 tctaccgcaa gtaacgcgtg gggacccaag cagtgaccga taaaacctct cttagctaca
    57901 aagatgccgg tgttgatatt gacgcgggta atgctctggt tggaagaatc aaaggcgtag
    57961 tgaagaaaac gcgtcgtccg gaagtgatgg gcggtctggg cggcttcggt gcgctgtgtg
    58021 cattgccgca aaaatatcgt gaacccgtgc tggtttccgg cactgacggc gtaggtacca
    58081 agctgcgtct ggcaatggac ttaaaacgtc acgacaccat tggtattgat ctggtcgcca
    58141 tgtgcgttaa tgacctggtg gtgcaaggtg cggaaccgct gtttttcctc gactattacg
    58201 caaccggaaa actggatgtt gataccgctt cagcagtgat cagcggcatc gcggaaggtt
    58261 gtctgcaatc gggctgttct ctggtgggtg gcgaaacggc agaaatgccg gggatgtatc
    58321 acggtgaaga ttacgatgtc gcgggtttct gcgtgggcgt ggtagaaaaa tcagaaatca
    58381 tcgacggctc taaagtcagc gacggcgatg tgctgattgc actcggttcc agcggtccgc
    58441 actcgaacgg ctattcgctg gtgcgcaaaa ttcttgaagt cagcggttgt gatccgcaaa
    58501 ccaccgaact tgatggtaag ccattagccg atcatctgct ggcaccgacc cgcatttacg
    58561 tgaagtcagt gctggagttg attgaaaagg tcgatgtgca tgccattgcg cacctgaccg
    58621 gcggcggctt ctgggaaaac attccgcgcg tattgccaga taatacccag gcagtgattg
    58681 atgaatcttc ctggcagtgg ccggaagtgt tcaactggct gcaaacggca ggtaacgttg
    58741 agcgccatga aatgtatcgc accttcaact gcggcgtcgg gatgattatc gccctacctg
    58801 ctccggaagt ggacaaagcc ctcgccctgc taaatgccaa cggtgaaaac gcgtggaaaa
    58861 tcggtatcat caaagcctct gattccgaac aacgcgtggt tatcgaataa tgaatattgt
    58921 ggtgcttatt tccggcaacg gaagtaattt acaggcaatt attgacgcct gtaaaaccaa
    58981 caaaattaaa ggcaccgtac gggcagtttt cagcaataag gccgacgcgt tcggccttga
    59041 acgcgcccgc caggcgggta ttgcaacgca tacgctcatc gccagcgcgt ttgacagtcg
    59101 tgaagcctat gaccgggagt tgattcatga aatcgacatg tacgcacccg atgtggtcgt
    59161 gctggctggt tttatgcgca ttctcagccc ggcgtttgtc tcccactatg ccgggcgttt
    59221 gctgaacatt cacccttctc tgctgccgaa atatcccgga ttacacaccc atcgtcaggc
    59281 gctggaaaat ggcgatgaag agcacggtac atcggtgcat ttcgtcaccg atgaactgga
    59341 cggtggcccg gttattttac aggcgaaagt cccggtattt gctggtgata cggaagatga
    59401 catcaccgcc cgcgtgcaaa cccaggaaca cgctatttat ccactggtga ttagctggtt
    59461 tgccgatggt cgtctgaaaa tgcacgaaaa cgccgcgtgg ctggatggtc aacgtctgcc
    59521 gccgcagagc tacgctgccg acgagtaatg cccccgtagt taaagcgcca gctctgccgc
    59581 tggcgttttt caattcacct gtaaatcgca agctccagca gtttttttcc cccttttctg
    59641 gcatagttgg acatctgcca atattgctcg ccataatatc caggcagtgt cccgtgaata
    59701 aaacggagta aaagtggtaa tgggtcagga aaagctatac atcgaaaaag agctcagttg
    59761 gttatcgttc aatgaacgcg tgcttcagga agcggcggac aaatctaacc cgctgattga
    59821 aaggatgcgt ttcctgggga tctattccaa taaccttgat gagttctata aagtccgctt
    59881 cgctgaactg aagcgacgca tcattattag cgaagaacaa ggctccaacg ctcattcccg
    59941 ccatttactg ggcaaaattc agtcccgggt gctgaaagcc gatcaggaat tcgacggcct
    60001 ctacaacgag ctattgctgg agatggcgcg caaccagatc ttcctgatta atgaacgcca
    60061 gctctccgtc aatcaacaaa actggctgcg tcattatttt aagcagtatc tgcgtcagca
    60121 cattacgccg attttaatca atcctgacac tgacttagtg cagttcctga aagatgatta
    60181 cacctatctg gcggtggaaa ttatccgtag cgatagcatc cgttacgcgc tgctggagat
    60241 cccatcagat aaagtgccgc gctttgtgaa tttaccgcca gaagcgccgc gtcgacgcaa
    60301 gccgatgatt cttctggata acattctgcg ttactgcctt gatgatattt tcaaaggctt
    60361 ctttgattat gacgcgctga atgcctattc aatgaagatg acccgcgatg ccgaatacga
    60421 tttagtgcat gagatggaag ccagcctgat ggagttgatg tcttccagtc tcaagcagcg
    60481 tttaactgct gagccggtgc gttttgttta tcagcgcgat atgcccaatg cgctggttga
    60541 agtgttacgc gaaaaactga ctatttcccg ctacgactcc atcgtccccg gcggtcgtta
    60601 tcataatttt aaagacttta ttaattttcc caatgtcggc aaagccaatc tggtgaacaa
    60661 accactgccg cgtttacgcc atatttggtt tgataaagcc cagttccgca atggttttga
    60721 tgccattcgc gaacgcgatg tgttgctcta ttatccttat cacacctttg ggcatgtgct
    60781 ggaactgctg cgtcaggctt cgttcgaccc gagcgtactg gcgattaaaa ttaacattta
    60841 ccgcgtggcg aaagattcac gcatcatcga ctcgatgatc cacgccgcac ataacggtaa
    60901 gaaagtcact gtggtggttg agttacaggc gcgtttcgac gaagaagcca acattcactg
    60961 ggcgaagcgc ctgaccgaag caggcgtgca cgttatcttc tctgcgccgg ggctgaaaat
    61021 tcacgccaaa ctgttcctga tttcacgtaa agaaaacggt gaagtggtcc gttacgcaca
    61081 catcgggacc gggaacttta acgaaaaaac cgcgcgtctt tatactgact attcgttgct
    61141 gaccgcagat gcgcgtatca ccaacgaagt acggcgggta tttaacttta ttgaaaaccc
    61201 ataccgcccg gtgacatttg attatttaat ggtgtcaccg caaaactctc gccgtctgtt
    61261 atatgaaatg gtagaccgcg aaatcgccaa cgcgcagcaa gggctgccca gtggtatcac
    61321 cctgaagcta aataaccttg tcgataaagg cctggttgat cgtctgtatg cggcctccag
    61381 ctccggcgta ccggttaatc tgctggttcg cggaatgtgt tcgctgatcc ccaatctgga
    61441 aggcattagc gacaacattc gtgccatcag tattgttgac cgttaccttg aacatgaccg
    61501 ggtttatatt tttgaaaatg gcggcgataa aaaggtctac ctttcttccg ccgactggat
    61561 gacgcgcaat attgattatc gtattgaagt ggcgacaccg ctgctcgatc cgcgcctgaa
    61621 gcagcgggtg ctggacatca tcgacatatt gttcagcgat acggtcaaag cacgttatat
    61681 cgataaagaa ctcagtaatc gctacgttcc ccgcggcaat cgccgcaaag tacgggcgca
    61741 gttggcgatt tacgactaca tcaaatcact cgaacaacct gaataaccct atgccaatac
    61801 acgataaatc ccctcgtccg caggagtttg ctgcggtcga tcttggttca aacagttttc
    61861 acatggtcat agcccgtgtg gtagatggtg ccatgcagat tattggccgc ctgaaacagc
    61921 gggtgcatct ggcggacggc ctggggccag ataatatgtt gagtgaagag gcaatgacgc
    61981 gcggtttgaa ctgtctgtcg ctgtttgccg aacggctaca agggttttct cctgccagcg
    62041 tctgtatagt cggcacccat acgctgcgtc aggcgctgaa cgccactgac tttctgaaac
    62101 gcgcggaaaa ggtcattccc tacccgattg aaattatttc cggtaatgaa gaagcccgtc
    62161 tgatttttat gggcgtggaa catacccaac cggaaaaagg tcgcaaactg gttattgata
    62221 ttggcggcgg atctacggaa ctggtgattg gtgaaaattt cgaacctatt ctcgttgaaa
    62281 gccgccggat gggttgtgtc agctttgccc agctttattt tcctggcggg gtcatcaata
    62341 aagagaattt tcagcgcgct cgcatggcgg cagcacaaaa actggaaact ttaacctggc
    62401 aattccgtat tcagggctgg aacgttgcaa tgggcgcttc cggtaccata aaagccgccc
    62461 atgaagtgtt aatggaaatg ggcgagaaag acgggataat taccccggaa cgtctggaaa
    62521 aactggtaaa agaaatttta cgtcaccgta atttcgcatc gctgagttta ccgggtcttt
    62581 ccgaagagcg gaaaacagtc ttcgttcctg gactggcgat tttatgtggt gtgtttgatg
    62641 ctttagccat ccgtgaactg cgcctttctg acggggcgtt acgcgaaggc gtactgtatg
    62701 aaatggaagg acgtttccgt catcaggatg tgcgtagtcg caccgccagc agcctcgcca
    62761 accagtatca catagacagc gaacaggccc gacgagtgct ggataccact atgcaaatgt
    62821 acgaacagtg gcgggaacag caaccgaagc tggcgcatcc gcaactggag gcgctactgc
    62881 gatgggccgc catgctgcat gaggtcgggt tgaatatcaa ccacagcggt ttgcatcgcc
    62941 actccgctta tattctgcaa aacagtgact tgccgggttt taatcaggaa cagcagctga
    63001 tgatggcgac actgctgcgc tatcaccgta aagcgattaa gctcgacgat ctgccgcgct
    63061 ttaccttgtt taagaagaaa cagttcctgc cactgataca gctattgcgc cttggcgtat
    63121 tactaaacaa tcaacgtcag gcaaccacca caccgccaac attgacactg attactgatg
    63181 acagtcactg gacactgcgt ttcccgcatg actggtttag tcagaatgcg ctggtactgc
    63241 ttgatctgga aaaggagcaa gaatactggg aaggcgtggc tggctggcgg ttgaaaattg
    63301 aagaagaaag taccccagaa atcgccgctt aatgctttgc gggccgacga gaaatgtcgg
    63361 cccgcattat tcaggcactt tcgcgaatgg gttcgatttc attcagcgta tcaattaacg
    63421 gttgcggctt accaataaga taaccctgca tataatcgat ccccaaagag agcaccgcct
    63481 cgcggatctc ttcgttttca acgtactctg ccactacccg cattttcttc attcgcgcca
    63541 gatggcaaat cgacgccaca atctgataat ccagactatt tgacacaata ttgcggataa
    63601 agctgccgtc aattttaagc agatcggcat tcacattttt aagccgtgca tagctggcgt
    63661 agccggtgcc gaaatcatca atcgcaatct ggcagcctaa ttcctgaaga tgctgcaagg
    63721 tgatttgcgc ctgcttaaca ttggtcagag cattactttc ggtgacttca aaaataagtt
    63781 gccacgcttc aatctgatat ttagccagca actgactgac ttcaacagga aaacgagcct
    63841 gacataccga ggttggagac agattaatag caaaacggtg agcgggcatc ttcgctctgt
    63901 tttcagccat aaattgcagc gtatgctcaa tgacccacat gtcgatactc gacgataaac
    63961 caaattcgtg cgcgaccggc aagaagctat cggggccgat cagttcatca ttctcacctt
    64021 tcatgcgcag aagaatttca tggtaaacat caccacgcat accggtaatc ggctgggcca
    64081 tcaggaaaaa atggttgtgc tccagcgcct gctgtagtcg attcatcatc gcgactttat
    64141 ctttcaattc gcgttgcaaa tacattgccc cgcaacgctg catattttcc ggggcgttgg
    64201 tcacgatgga aagttcggcg accgtattta gctctcccag cagcaggtag atatgattca
    64261 ctggcgagcg cacatagcag taactgacgc caatctgcgg ttgcatcggc atcccatccc
    64321 aaaagaaacg aaattgcttg agatgactat ccagtgcggt aatgcgctcc tggtgcgatt
    64381 ctgtattcag tcgcagcgcg agatcgttac ccgaaagctg ataaacatct tcacccggtt
    64441 ccagcaaggg tgacagccag tgagaaagtt tttgcttgt
//
LOCUS       DADWTH010000011        60685 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.011, whole genome shotgun sequence.
ACCESSION   DADWTH010000011 DADWTH010000000
VERSION     DADWTH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.011"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..320
                     /locus_tag="JNY02_000691"
     CDS             <1..320
                     /locus_tag="JNY02_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9432343.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="JNY02_000692"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="JNY02_000692"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBB9432344.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="JNY02_000693"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="JNY02_000693"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBB9432345.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVGLA"
     gene            complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="JNY02_000694"
     CDS             complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="JNY02_000694"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBB9432346.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="JNY02_000695"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="JNY02_000695"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBB9432347.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="JNY02_000696"
                     /pseudo
     CDS             complement(3724..4953)
                     /locus_tag="JNY02_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(5017..5457)
                     /locus_tag="JNY02_000697"
     CDS             complement(5017..5457)
                     /locus_tag="JNY02_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB9432348.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="JNY02_000698"
     CDS             complement(5562..6332)
                     /locus_tag="JNY02_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9432349.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="JNY02_000699"
     CDS             complement(6329..7255)
                     /locus_tag="JNY02_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB9432350.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="JNY02_000700"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="JNY02_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBB9432351.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNY02_000701"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNY02_000701"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBB9432352.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /gene="gcd"
                     /locus_tag="JNY02_000702"
     CDS             8809..11199
                     /gene="gcd"
                     /locus_tag="JNY02_000702"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBB9432353.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNY02_000703"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNY02_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="JNY02_000704"
     CDS             12961..13308
                     /locus_tag="JNY02_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBB9432354.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="JNY02_000705"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="JNY02_000705"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBB9432355.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQQS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="JNY02_000706"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="JNY02_000706"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBB9432356.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="JNY02_000707"
     CDS             complement(15128..15490)
                     /locus_tag="JNY02_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBB9432357.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNY02_000708"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNY02_000708"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBB9432358.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIINRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="JNY02_000709"
     CDS             18618..20471
                     /locus_tag="JNY02_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBB9432359.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="JNY02_000710"
     CDS             complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="JNY02_000710"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBB9432360.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPNPNPKAKKK"
     gene            complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="JNY02_000711"
     CDS             complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="JNY02_000711"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBB9432361.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="JNY02_000712"
     CDS             complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="JNY02_000712"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBB9432362.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="JNY02_000713"
     CDS             complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="JNY02_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBB9432363.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28345..29718
                     /gene="aroP"
                     /locus_tag="JNY02_000714"
     CDS             28345..29718
                     /gene="aroP"
                     /locus_tag="JNY02_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBB9432364.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="JNY02_000715"
     CDS             complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="JNY02_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBB9432365.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="JNY02_000716"
     CDS             complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="JNY02_000716"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBB9432366.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31251..32144
                     /gene="nadC"
                     /locus_tag="JNY02_000717"
     CDS             31251..32144
                     /gene="nadC"
                     /locus_tag="JNY02_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBB9432367.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32347..32787
                     /gene="ppdD"
                     /locus_tag="JNY02_000718"
     CDS             32347..32787
                     /gene="ppdD"
                     /locus_tag="JNY02_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBB9432368.1"
                     /translation="MDKQHGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32797..34182
                     /gene="gspE"
                     /locus_tag="JNY02_000719"
     CDS             32797..34182
                     /gene="gspE"
                     /locus_tag="JNY02_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBB9432369.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34172..35374
                     /gene="hofC"
                     /locus_tag="JNY02_000720"
     CDS             34172..35374
                     /gene="hofC"
                     /locus_tag="JNY02_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBB9432370.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="JNY02_000721"
     CDS             complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="JNY02_000721"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBB9432371.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36614..36652)
                     /locus_tag="JNY02_000722"
     CDS             complement(36614..36652)
                     /locus_tag="JNY02_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432372.1"
                     /translation="MIIKTLKMSARK"
     gene            36677..37297
                     /gene="coaE"
                     /locus_tag="JNY02_000723"
     CDS             36677..37297
                     /gene="coaE"
                     /locus_tag="JNY02_000723"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBB9432373.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37297..38040
                     /gene="zapD"
                     /locus_tag="JNY02_000724"
     CDS             37297..38040
                     /gene="zapD"
                     /locus_tag="JNY02_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBB9432374.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38050..38247
                     /gene="yacG"
                     /locus_tag="JNY02_000725"
     CDS             38050..38247
                     /gene="yacG"
                     /locus_tag="JNY02_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBB9432375.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="JNY02_000726"
     CDS             complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="JNY02_000726"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBB9432376.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38796..41501)
                     /gene="secA"
                     /locus_tag="JNY02_000727"
     CDS             complement(38796..41501)
                     /gene="secA"
                     /locus_tag="JNY02_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBB9432377.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41563..42075)
                     /gene="secM"
                     /locus_tag="JNY02_000728"
     CDS             complement(41563..42075)
                     /gene="secM"
                     /locus_tag="JNY02_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBB9432378.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="JNY02_000729"
     CDS             complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="JNY02_000729"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBB9432379.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVCVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="JNY02_000730"
     CDS             complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="JNY02_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBB9432380.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="JNY02_000731"
     CDS             complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="JNY02_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBB9432381.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="JNY02_000732"
     CDS             complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="JNY02_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBB9432382.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46627..47547)
                     /gene="ddlB"
                     /locus_tag="JNY02_000733"
     CDS             complement(46627..47547)
                     /gene="ddlB"
                     /locus_tag="JNY02_000733"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBB9432383.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47540..49015)
                     /gene="murC"
                     /locus_tag="JNY02_000734"
     CDS             complement(47540..49015)
                     /gene="murC"
                     /locus_tag="JNY02_000734"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBB9432384.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49069..50136)
                     /gene="murG"
                     /locus_tag="JNY02_000735"
     CDS             complement(49069..50136)
                     /gene="murG"
                     /locus_tag="JNY02_000735"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBB9432385.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="JNY02_000736"
     CDS             complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="JNY02_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBB9432386.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51377..52693)
                     /gene="murD"
                     /locus_tag="JNY02_000737"
     CDS             complement(51377..52693)
                     /gene="murD"
                     /locus_tag="JNY02_000737"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBB9432387.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="JNY02_000738"
     CDS             complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="JNY02_000738"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBB9432388.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53772..55130)
                     /gene="murF"
                     /locus_tag="JNY02_000739"
     CDS             complement(53772..55130)
                     /gene="murF"
                     /locus_tag="JNY02_000739"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBB9432389.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55127..56614)
                     /gene="murE"
                     /locus_tag="JNY02_000740"
     CDS             complement(55127..56614)
                     /gene="murE"
                     /locus_tag="JNY02_000740"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBB9432390.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56601..58367)
                     /gene="ftsI"
                     /locus_tag="JNY02_000741"
     CDS             complement(56601..58367)
                     /gene="ftsI"
                     /locus_tag="JNY02_000741"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBB9432391.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="JNY02_000742"
     CDS             complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="JNY02_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBB9432392.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="JNY02_000743"
     CDS             complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="JNY02_000743"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBB9432393.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="JNY02_000744"
     CDS             complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="JNY02_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBB9432394.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCAIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14536 a        16811 c        14922 g        14416 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcgg gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggctccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc taggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgaggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccatgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcaatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gcagtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttaa taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cacgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcgggttcgg
    20581 gttcggcagg tcggtaatgc taccttcgaa cacttctgcc gccaggccca cagactcgtg
    20641 cagagtcggg tgcgcgtgga tggtcagtgc gatgtcttca gcatcacaac ccatttcgat
    20701 tgccagcccg atttcaccca gcagctcgcc gccgttggta ccaacaatcg caccaccgat
    20761 cacgcggtga gattctttgt cgaaaatcag cttggtcata ccgtctgcgc agtcagaagc
    20821 gatagcacga ccagaagcag cccacgggaa ggtggcggtt tcatagctga tgcctttctc
    20881 tttcgcttct ttctcagtca gacctaccca tgcaacttct ggttcggtat aggcgatgga
    20941 cgggataact ttcggatcga agtagtgttt cttaccggcg ataacttcag cggcaacgtg
    21001 accttcgtga acacctttgt gtgccagcat cggctgaccg acgatatcgc cgatagcaaa
    21061 gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg atgaaaccac ggtcgtcaac
    21121 ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac caatcgctac
    21181 cagcacggcg tcgtaacgct gcggttcagc tggtgctttt ttgccttcca tcgtcacata
    21241 aataccgtct tctttcgctt caacggcggt aactttggtt tccagcatca ggttgaattt
    21301 cttgctgata cgcttggtga agactttaac gatgtctttg tcagctgccg ggataacctg
    21361 gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtaaacgg tgcccatttc
    21421 cagaccgatg ataccgccac ccattaccag caggcgttct ggcacttctt tcagttccag
    21481 cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga ataaacggca gttggatcgg
    21541 gcgagaaccc gctgcaatga tcgcattgtc gaagttgatc acggttttgc cgttctcacc
    21601 ttcaacttcc agggtgttag ccccggtgaa tttacccaga ccgttgacca ctttgacttt
    21661 gcggcctttc gccataccag ccagaccacc ggtcagctga ttgatcactt tctctttcca
    21721 ggtacgaatc ttgtcaatat cggttttcgg ttcgccgaag acgataccgt gttcagccag
    21781 cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag ggatacagcc
    21841 gacgttcagg caaacaccgc caagggtgtt gtaacgttct acgattacgg tttccagacc
    21901 taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa gtaccacgac
    21961 ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg cggtcatacc
    22021 cgtcgtcttt caggccgcag cggcgttagc ttcacgccct caccccagtc acttacttat
    22081 gtaagctcct ggggattctg gcgcttgcta ccttgctgca cccagaaatc catagggtat
    22141 gtgaagtaat accctaacca ccaccgggtc gttctatccg tcggtatttt acaaaattgt
    22201 taacaatttt taaacaacaa acggcaaccg atttgtctat tcgctaataa cctcaataca
    22261 ttcatgagat taccagaaaa aagccggccg ttgggccggc tcttttactt acatcaccag
    22321 acggcgaatg tcagacagcg tgttgttaat gatggtaatg aaacgggcac catcagcacc
    22381 gtcgatcacg cggtggtcga aggagagaga aatcggcagc atcagacgcg gcacgaactc
    22441 tttaccattc cacaccggct ccatcgcgga cttggaaacg ccgaggatag ccacttccgg
    22501 cgcgttcaca atcggcgcga agtgggtagt acccaggccg ccgatgctgg agatggtgaa
    22561 gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct tagaaatagt
    22621 catcagctcg cgagacagct cgatgatgcc tttcttgttg acgtctttga ataccggaac
    22681 aaccagaccg ttcggggtat ccaccgccac accgatgttg atgtatttct tcagggtcag
    22741 acgctgaccg tcttccgaca gcgaactatt gaagcgaggc atctgctcaa gagctgcagc
    22801 aacggctttc atgatgaaga caaccggggt gatcttcaca tccagcttac gtttcgccgc
    22861 ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg
    22921 ggtaacatgc gggatcatca cccagttacg gctcaggtta gcaccagaaa ttttctggat
    22981 acggcccagt tccacttctt cgatttcgcc aaacttgctg aagtccacct tcggccacgg
    23041 cagcatgcca gggataccac cgccagtcgc cgccggagct gcttctgcac gtttgatagc
    23101 ttctttcacg taagcctgaa cgtcttcgcg caggatacga cctttacggc cagtgccctt
    23161 cactttcgcc aggttaacgc caaactcgcg tgccagacgg cggatcagcg gggtcgcgtg
    23221 aacgtaagcg tcgttttcag caaattcaga tttgccttcc gctttcgcag ccggagctgc
    23281 tgccggagct tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc
    23341 tgccgcaggc gctgcgcctt caacttcgaa gatcataatc agcgaaccag ttttcacttt
    23401 atcgccaacg ttgactttca gttccttcac gacgcctgca aacggagccg gaacttccat
    23461 agaagcttta tcgccttcta cggtaatcag tgactgttca gcggcaactt tgtcgcccac
    23521 tttcaccatc acttcggtca cttcaacttc gtcaccgccg atatccggaa cgttaacttc
    23581 tttcacgcca gctgctggtg cgggggtcgc tgccggagtc gcttcctgtt tagccgccgg
    23641 agctgccgcg cctgcttcac ccgcgacttc gaagaccata atcagcgagc cggtagacac
    23701 tttgtcaccc acgttcactt tgatctcttt cacggtacct gcaaacggag ccggaacttc
    23761 catagaagcc ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc
    23821 aactttcacc aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac
    23881 gtcttttgcc gccgcagccg cgggtgctgc tgccggagct gcttctttct tctcttctgc
    23941 ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc
    24001 ggtctgggtt ttatcgccaa cagagacttt gatctcttta acgatacccg cctgcggaga
    24061 tggaacttcc atagaggctt tgtcgccttc tacggtgatc agcgactgtt cggcttcaac
    24121 tttgtcgccc actttgacca ggatctcggt gatttcaact tcatcagccc cgatgtccgg
    24181 tactttgatt tcgatagcca ttattctttt acctcttacg ccagacgcgg gttaacttta
    24241 tctgcatcga tgttgaattt ggcgattgcg tcagcaacca ctttcttatc gatttcgcca
    24301 cgtttagcca gttcgcccag cgccgcaacc acgacataag aagcatcaac ttcgaagtgg
    24361 tgacgcaggt tctcacggct gtcggaacga ccgaagccat cagtacccag tacgcgatag
    24421 tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca gtttcatata gtcggtagat
    24481 gccactgccg gcgcgtcgtt catcacctga gcgatatacg gaacgcgcgg agtttccagc
    24541 ggatgcagca tgttccagcg ttcacaatcc tgaccatcac gcgccagctc ggtgaaggag
    24601 gtcacgctat aaacgtcaga acctacgccg taatctttcg ccaggatctc tgccgcttca
    24661 cggacgtgac gcaggatgga accggagccc agcagctgaa ctttaccttt gctaccttca
    24721 atggtttcga gtttgtagat acctttacgg ataccttcct cagcaccttc cggcattgcc
    24781 ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt aaacgttctc ttgtttttca
    24841 ccgtacatac gctccagacc gtcatgcata atgacagcaa cttcgtaagc gtaagccggg
    24901 tcgtaagaga tacagttcgg gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc
    24961 tgcagacctt caccgttcag ggtggtacga ccggaagtac cgccgatcag gaagccacgc
    25021 gcttgctggt ctccagccgc ccagcacaga tcgccgatac gctggaagcc gaacatcgag
    25081 taatagatgt agaatgggat catcggcaga ttgttcgtgc tgtaagaggt cgccgctgcc
    25141 agccaggaac accctgcgcc cagctcgttg atcccttcct gcagaatctg acctttctcg
    25201 tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg
    25261 ctgtaaatac caatctgacg gaacagacct tccataccga aagtacgcgc ttcgtcggcg
    25321 atgatcggta ccagacgatc tttgatcgac ttgttcttca gcatcacgtt cagagcacga
    25381 acaaaagcga tagtggtaga gagctctttg ctctgctctt ccagcagcgc gccgaagtct
    25441 tgcaggctcg gcagctcaag cttctcggtg aagttcggct gacggcttgg cagataaccg
    25501 tgcagtttct gacgctgagc gtgcagatag gtatgctctt cagaaccttc cgggaaggtg
    25561 atgtacggca gtttttcgat atctgcatca gacaccggca cattgaaacg gtcgcggatg
    25621 tgacgcacgc cgtccatgtt cattttctta acctggtgcg cgatgttttt accttcagcc
    25681 gcgtcgccca tgccgtaacc tttaatggta tgagcaagga ttactgtcgc tttgcctttg
    25741 gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc accacggttc
    25801 agtgcccaga tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc aggatattta
    25861 ccgaagaagt gttcacgaac gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg
    25921 tcaacggttt cgttcatcag ctggatcagt ttaccgctgg tatctttacg cagcagttca
    25981 tcccaacggc taccccacat cactttgatc acgttccagc cagcaccttc gaagatgcct
    26041 tccagttcgt tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta
    26101 cagttgataa cgaaaaccag gttatccagt ttttcacggg tagcgatggt gatcgcacct
    26161 ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag
    26221 gtatctttca ggccacggtg ttccagatat ttcaggaatt tagcctggta aatagcacca
    26281 atcggaccca gacccataga tacggtcggg aactgccaga attccggcat cagtttcggg
    26341 tgcggatagg aagagaggcc attgccgtga acttcctgac ggaagttatc cagctgctcc
    26401 tgagtcagac gaccttccag gaaagcacgg gcgtaaacgc ccggggagat gtggccctgg
    26461 aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac
    26521 acatcataaa tggttgcgga agactggaag gacgccatgt ggccgcccag ttcgaggtct
    26581 tttttcgacg cacgcagcac cgtcatgatg gcgttccagc ggatagctga acgaatacgg
    26641 cgttccagtt ccagattacc cggatactcc ggttgttctt caacggggat ggtgttgatg
    26701 tagttgctga tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc ttcagcaagc
    26761 agttggtcga tcagatactg agcacgctca acaccttctt cacggatgac cgattcgatc
    26821 gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg
    26881 ggttattcct tatctatcta ataacgttga gttttctgga acctgtccca ttgaactctc
    26941 gccagaaagc tcaataagac aggttctacg tttagttacc gcgcttttat atgcgcttga
    27001 tttacaacat cttctggata atttttacca gaaaaatcac taattctttc gttgctccag
    27061 acgacgcaga gaacgctcac ggcggctctc ttcacggctt ctgtcgagca aaatttcttc
    27121 gataaaggcc agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat
    27181 cgcttcaaat atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata
    27241 gagcaattcg aagttctggc ggacattctg ggccaacatc ggctccatac accttagcag
    27301 atgaagcaga accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc
    27361 gtttgattcc gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc
    27421 acggatgcgt tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc
    27481 ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt cggagagcag
    27541 ctccaccagc ggatcgctga agctttgcca taggctgctc tggacaaaag tgccgccacc
    27601 ctggcgacga agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaaggaggg
    27661 acgggagacg tcgaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg
    27721 gagagtgcct tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg
    27781 ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg aagtcaattg
    27841 gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag tattcacctt atgtccatac
    27901 aggttttgat tgaaatcatg aaactgtgca cattttaaca acttgacata tataacgttt
    27961 caaagttgta actatgcaca aatgtagact ttacgtagga aagggatttt ttaacgatta
    28021 ttaaactata aaaatccgaa ttgaaccgat tcacttacca attttttgat ttttaagtca
    28081 ataaaaacga atttaaattc actctgcata ttgagagggg ttgaggctga gctttacaaa
    28141 cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat tcgctgccgc
    28201 ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaactt ccttgatgta
    28261 aataaatcaa tacaacaaac ggaattgcaa acttacacac gcatcactgc gtagatcaaa
    28321 aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga gcagctaaag
    28381 cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat agggacaggg
    28441 ctattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat cctgggttac
    28501 gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat ggtggtcgaa
    28561 gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag ttttgccggt
    28621 ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc agagctgact
    28681 gccgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt ttctgccgcc
    28741 gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt tggcgagatg
    28801 gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat cttcggcggc
    28861 tggctgctgt tcagtggcaa cggcggcccg caggcgagcg ttagcaacct gtgggatcaa
    28921 ggtggtttcc tgccgcacgg cttcaccgga ctggtgatga tgatggcgat tatcatgttc
    28981 tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa cccggagcaa
    29041 agtatcccga aagcaactaa ccaggttatc taccgcatcc tgattttcta tattggctcg
    29101 ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac cagtccgttt
    29161 gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa catcgtggta
    29221 ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg tatgctgttt
    29281 ggtctggcac aacaggggaa cgcgccaaaa gcgctggcgt ctgtcgataa acgcggcgta
    29341 ccggttaaca ccattctggt gtctgcgctg gttacagcat tgtgcgtatt gattaactat
    29401 cttgctccgg aatccgcatt tggcctgtta atggcactgg tggtatccgc actggtgatc
    29461 aactgggcga tgatcagtct ggcgcatatg aagttccgtc gcgccaagca ggaacaaggc
    29521 gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg cctgctgttt
    29581 atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc ggtatacctg
    29641 atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa aaccgccaaa
    29701 gccgtaaaag cgcattaatc tctctacgcc ctcacccgta cagggtgagg gcaataatct
    29761 ctacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca cgaacgaggt
    29821 tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat gcggttcacg
    29881 cgccagagag aactgcgcca gacgcgttaa tacctgatat tgcgaagtat ggaaatcacc
    29941 cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg catataccac
    30001 acccgcaaga cgaaccggca cccaatccag tacatgaagc actgcatcaa tgccggactg
    30061 taaacgatga tgcggcgtct ggtatctcgc cagccagtat tgccatgcac gcaagaacgc
    30121 ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga acagcggtgc
    30181 aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca aatactcacg
    30241 ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca tcgtggcacg
    30301 ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa gacgaacttt
    30361 acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg gaacgttgaa
    30421 caatactccc tgcaatgcgc gtaacagtaa aaaagtcaca cccatcgcaa taatggtcat
    30481 gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt caagacgatg
    30541 atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca ccagtaaggt
    30601 tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc ccaatcaaat
    30661 gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt catgttatta
    30721 gcgatatcgg gatagcgatc tatcagtgcc cgcgtaaccg ccgcaagctg ttgatactgc
    30781 gcatcggtat acgccagagt atcggtgcct tcaagctcaa tcccaataga aaaatcattg
    30841 cagcgttcac gcccctgata ctgagagact cccgcatgcc aggcacgttt atcgaaagga
    30901 acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac gcgcaaatgg
    30961 gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa taatgcgtcg
    31021 atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac cagcagggtg
    31081 ggtgtttcgt catccaggcg gcaatcgtaa tgtggtgagg gaacgcggcg cgcgccaacc
    31141 agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa tacccggttc
    31201 agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac atgccgcctc
    31261 gccgctacaa ccctgacacc cgacgtgacg agctgctgga acgcattaat ctcgatatcc
    31321 ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat gccaacaatg
    31381 acattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg atcacccgcg
    31441 agaatggcgt attttgtggc aaacgctggg ttgaagaggt gtttattcaa ctggcaggcg
    31501 acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc aatcaaccct
    31561 tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact gcgcttaatt
    31621 ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa ttactggaag
    31681 gcaccaacac gcagttgttg gatacgcgca aaaccttacc tggcctgcgt tcagctctga
    31741 aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct gatgccttcc
    31801 tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc gaaaaagcgt
    31861 cctggctgca tccggatgcg ccagtagaag tcgaagtaga gaatctggaa gaacttgatg
    31921 aggccctgaa agccggagcc gatatcatca tgctggataa cttcgaaaca gaacagatgc
    31981 gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc aacgtcactg
    32041 acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc ggcgcgctaa
    32101 ctaaacacgt acaagcactc gacctttcaa tgcgttttcg ctaattgctg acagatttat
    32161 ccgctccatc gcgggcggat aatctcccct tccccaactt tcttcgtaac gcctcgcaaa
    32221 tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc cgtttcgttt
    32281 tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa tcaaggagcg
    32341 aaacagatgg acaagcaaca cggttttaca cttatcgaac tgatggtggt tattggcatc
    32401 attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg caaagccgca
    32461 ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt gtgcgcgctg
    32521 gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc
    32581 acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac cgggcaagaa
    32641 agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc
    32701 ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat
    32761 gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca gctcacggcc
    32821 ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt tcatgttgcg
    32881 gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac caccaaacgt
    32941 attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg cacacaacag
    33001 acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct gactcgaacg
    33061 ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc ggacaatgcc
    33121 taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat
    33181 gccggagtcg cattaacagc cagattaaaa gtgctgggaa acctggatat tgcggaacat
    33241 cgcctgccgc aggacggcca attcactgtc gaactggcag gaaacaccgt ctcatttcgt
    33301 attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg
    33361 agccaagcac tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct
    33421 catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa
    33481 acggtcacgc tttatagtgc cctgcaaacg ctgaataccg ctgacattaa tatttgtagc
    33541 gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt
    33601 gccgggctca cctttcaggg tgttttgcgt gcgttattgc gccaggatcc tgacgtcatc
    33661 atgatcggag agatccgcga tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc
    33721 ggtcacctgg tgttgtccac gctacacacc aattccacca ctgaaacgct ggtacgttta
    33781 cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt aatagcccag
    33841 cgtctggtac gtaaactttg cccacattgt cgccgacagc aaggggagcc catccatatt
    33901 ccagacactg tatggccgtc gccgctgccc cactggcagg cacccggttg tgtacattgc
    33961 taccacggtt tttatggtcg taccgccttg tttgaagtcc tgcccattac accagccata
    34021 cgccagctta tttccgctaa tactgaagtt gaatcgctgg aaacgcacgc ccgacaggcg
    34081 ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt aaccaccttt
    34141 gaagagttaa tccgcgtact ggggatgccg catggcaagt aagcaactct ggcgctggca
    34201 tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga gccgtacttt
    34261 actgcttatg gcactacagc aacagatggt taccccacta agcctgaagc gaatcgccat
    34321 caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct
    34381 acttaaagcc gggttaacgc tttctgaagg gctggctctg ctggcggaac agcatcccag
    34441 taaacaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt
    34501 ttccaatgcc ttattaccct ggtcagaggc atttccgccg ctctatcagg cgatgatccg
    34561 cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa
    34621 atcccagcgt cagttgaccg acaaagtgaa atcagcgttg cattatccca tcatcatttt
    34681 agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg agtttgccgc
    34741 tatctataag accttcaaca ccccactacc ggcactaacg caggggatca tgacgctggc
    34801 agactttagt ggcgaatggg gctggctgct ggtgttgttc ggctttctgc tggcgatagc
    34861 caataagttg ctgatgcgcc gcccgacctg gcttatcgcg cggcagaaat tgctgttacg
    34921 catcccgatt atgggttcac tgatgagggg acaaaaactc acgcagatct ttacgattct
    34981 ggcgctgaca caaagtgcag gcattacttt tttacagggc gtagagagcg tcagagaaac
    35041 aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg
    35101 tcatcccatc tggctggcgc taaaaaatgc cggtgagttt agcccgctct gtttgcaatt
    35161 agtgagaaca ggagaggcat ccggctcgct ggacctcatg ttagacaacc tcgcccatca
    35221 tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg aaccggcgtt
    35281 gctgatcata acgggaggaa ttatcggtac gctggtggtg gcaatgtatc tgccaatttt
    35341 ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc cacgccagcg
    35401 ttgggagatt acaggttgtt gaagatgcgg ttttcttgtt ccagcacacg aataaacgtg
    35461 gtgcgcttgg tcagctcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg
    35521 ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag cttgacggtt
    35581 ttaccttctg ctgcgcggta ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg
    35641 gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg
    35701 ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag
    35761 gctttcgcca catcgcccgg cgtggtacag ccaccgtcgc tgacgatcat tccgcccaga
    35821 ccgtgcgccg catcggcaca ttcgattacc gcagaaagct gcggataacc gacgcctgtt
    35881 ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcagcaccg
    35941 gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg
    36001 gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc
    36061 gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt
    36121 tcgaaatccg catcagacgt gccggtagaa accatcacat gtttcagcac atcagcggaa
    36181 gaattgttga taaacgcttg ccactcttcg acagaaaagt gtttatgcac agcagtcaga
    36241 atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc catatttgcg
    36301 gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa ttgacgttcc
    36361 agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta
    36421 aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata
    36481 agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact
    36541 gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa
    36601 cgtttcatca atttcacttt cgcgcgctca tttttaacgt tttgataatc atgatgaatt
    36661 cccgggaata taaattatgg ggtatatagt tgccttaacg ggaggcattg gcagtggtaa
    36721 gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat
    36781 tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg
    36841 cgaaaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt
    36901 cgccaacccg gaagagaaaa actggcttaa cgacctgctg catccgctga ttcagcaaga
    36961 gacgcaacac cagatccagc aagcaacttc cccctatgta ctgtgggttg tgccattgct
    37021 ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga
    37081 aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgaacatg tcgaacaaat
    37141 ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg tcattgataa
    37201 taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac actatttgca
    37261 gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa
    37321 catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc
    37381 accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt
    37441 gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttaac
    37501 cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt
    37561 gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc
    37621 gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca
    37681 ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg
    37741 cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt
    37801 accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat
    37861 ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat
    37921 tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg ttttatgccg
    37981 ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc ctgttgctaa
    38041 ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggtg
    38101 tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc
    38161 ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat
    38221 gactggagcg aagagccaaa gcagtgacat ttgtctgatg cctcacgtag gcctgataag
    38281 acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta
    38341 tccaacctac agacgcttaa gcttcgcaat taccggttca ttggctggcg gaaaatcatc
    38401 ggcattaaga ccgaccagcg acatccactc accgggttgc ccttctttac cccacggcac
    38461 cccttcccaa ctttcgacca gccagaacca cagagttata tgcctgtccg ggaattcata
    38521 ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacctctt cctgaagttc
    38581 acgcaccacc gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg gaaactccag
    38641 tttattcgcc atgtgcgcat ctgctgcgcg acgtgttata aagatttcat tgttctcgtt
    38701 gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg
    38761 cagaatcctg cgccttttac ttcaactgtt agcttttatt gcaggcgacc atggcactgc
    38821 ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt gcgttcaccg
    38881 gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct aagctgctgc
    38941 atttgtgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc aacctcttca
    39001 ggcatacgta cctgaacttt gctcagcgta ctgataactt catatttcaa cgactccagc
    39061 atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc tttctgtgca
    39121 tagccacgca ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg ctctttccac
    39181 agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat ctcagcacca
    39241 accacttctt ctttacgctg atacacttcg atggactgcg ccagaatgcg ctcacgcagc
    39301 gtctcttcat gcagttctgg ttctttatcc agccactcgg caattggtaa atcgaggtcg
    39361 aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc cagcgactgt
    39421 ggcggaatgt aggcatcaat ggtggctttg aacacatctt cacgaatgct gttgatggtt
    39481 tcgctcacat cgctgacatc cagcagttcg ttacgctggg agtaaatggc gcgacgctga
    39541 tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acgactttca
    39601 actttacgct gggcgttggc aatcgcttta gtcacccacg ggtgttcaat ggcttcgcct
    39661 ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc
    39721 agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa
    39781 cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc gatgatatgc
    39841 aggccacctg cttccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca
    39901 atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct acctccgagc
    39961 acaatatctg taccacgacc cgccatgttg gtcgcgatag tcaccgcagc cggataacct
    40021 gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac attgtgctta
    40081 ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga gatagtaccc
    40141 accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat cgcctgaatt
    40201 ttttccgctt cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg
    40261 gtcggaacaa cgacggtatc cagtttgtag atggagctaa attcgaaagc ttcggtatca
    40321 gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc
    40381 gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc cacagcctgg
    40441 tgcagaccat cggaccagcg tcggccctgc atggtacgac cggtgtgttc gtcaacgatg
    40501 ataacttcac catctttaac gatgtagtcg acgtcacggg taaacagcgc atgagcgcgc
    40561 agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag agactcccct
    40621 tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc acgttcggtc
    40681 aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg gaaggtttcg
    40741 gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg tttatacatt
    40801 tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc atcgatcagg
    40861 atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg ttcttcaggg
    40921 ctgaacgcca tgttgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagtg
    40981 atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag gttgatacca
    41041 acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg
    41101 tagtcgttga cggtaactac gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc
    41161 agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta
    41221 agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac gcgcttactt
    41281 gcctcacgca ccacggcgaa agcttccggg atcagatttt ccagcacttc gcctttttcc
    41341 agacgcgcac gaaactctgc ggttttccct ttcagttctt catcggagag tttttccatc
    41401 tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag ggtgcgatcg
    41461 ttacgactac cgaaaacttt agttaacaat ttgattagca taataaaatc ccaaacgccc
    41521 cgcgttacgg agttaataaa atgaagtaaa ggtttattgt tgttaggtga ggcgttgagg
    41581 gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt ttgcttgtgg
    41641 ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt cctgggtcag
    41701 cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca aagattcttc
    41761 agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga tgacggatta ccgtgcgaat
    41821 agcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgcg tgttcgcttc
    41881 cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc
    41941 ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc
    42001 gaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt
    42061 cagtattcca ctcacgttat tgccatcccg atcgaattca aacgtattta gcgcacaaat
    42121 cttatcatta aagcctgaag tccacagcac gaatgaagat aagttgtgcg taaaagtgct
    42181 taagaaaaca gcgttcgcac cgttttttat agctcttata aaattcaaaa aaaaacgact
    42241 ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg acagtataaa
    42301 tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc
    42361 tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag cagtttgtta
    42421 ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg accacacatg
    42481 aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc aaaacgcagg
    42541 ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa gctgccgccc
    42601 aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa cgtacgcgca
    42661 cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg ctggttgctg
    42721 gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc
    42781 ttaaattcag cccacttatc gccatcttcg acacaaacag tctctttgat gcgaacaaat
    42841 tttttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac aaacggagcg
    42901 gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat gttatcgatg
    42961 cccaggcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac atcatgctcg
    43021 ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc cgggaaatct
    43081 accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg cgcagggcgt
    43141 aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt
    43201 ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc aaccgaagtg
    43261 tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa ttccagtcaa
    43321 ttcttaatca gcttgcttac gtaggaatgc tgggatatcc agataatccg gctctttcgc
    43381 agtttgcggc gcattgtcat tcactacttt agcaaccggc ttctgctcct gggtcagcgg
    43441 agccatccca tgctgctggt agcgatccat cactggctgc tgaacctgct tattggtcac
    43501 cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg ttacgcgcag
    43561 ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt tgtcggaagc
    43621 aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc
    43681 cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg atatcttcca gcagcggaga
    43741 agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca cgccagaacc
    43801 catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt ccacgttcat
    43861 caaacccgga cgagtaatca gttcggcgat accttgcaca gcgcctttca gtacatcgtt
    43921 cgctgcgcca aacgcatcca gcaaggagat accgcggccc agaactttca gcagtttgtc
    43981 gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc cctgctcggc
    44041 gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag caacggtcag
    44101 gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac cggtaccacc
    44161 acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac gcaatgcatc
    44221 gcgatcctca tcagccgcat tgcggccaac ttctggatta gcgccagcgc ccagtccttt
    44281 ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc
    44341 atcggtattt accgcgaaga attcaacacc ttcaatacgc tcgcgcacca tgtgttcaac
    44401 agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat tggtaagttc
    44461 cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg
    44521 tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct tgatccacga
    44581 gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat gtgactcttt
    44641 cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat aatccgttaa
    44701 accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc gctgagcaca
    44761 ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg ccgccaggtg
    44821 atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt cgttgaccag
    44881 gttgagcaac tcggtatagc gcggctcgat cacctctgcc agggtctgac gttgcagact
    44941 ccgcggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc caacgatgga
    45001 acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa
    45061 ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct tagtgtggcg
    45121 caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat cgacgacgca
    45181 gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca gtccggcaaa
    45241 tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat
    45301 atcgttgtga catgtgatca ggtgcacttt tgcctgcatc cgcacacccg aaagtcctac
    45361 cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca catgcagcac
    45421 acgatgctca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt ccacatcttc
    45481 ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc agctgatgtg
    45541 cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca attctgcctg
    45601 gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc cgcctttatc
    45661 cataccacgt gacgggcagc tgcccacgcc aatgatattg accataccgt cgggcagaac
    45721 ttcccctact aaagcggcaa ccttcgcggt accaatttcc agtcctacta ccagttttct
    45781 gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg agtagattcc
    45841 tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa atcaacgtag
    45901 ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag
    45961 cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc gttattcagc
    46021 gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt
    46081 gccagcatct gccccatttc gcgatagccc tgcaacactt cattagcgct gccttccggg
    46141 ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt
    46201 ccttccgcgt ctaccatgtg ttgatcattc caccgcgcaa tcggcacata ttcaaccaga
    46261 tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat ccacggcagg
    46321 cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt acccggctca
    46381 cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc
    46441 aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagctcaa cacgacccag
    46501 ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt
    46561 ccattattgc ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag agcagcctgc
    46621 gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc tcatacctgc
    46681 ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg tattggcttc
    46741 cagcagataa aactgtccat cactgtccag cataacgtca atacgtcccc atcctttgca
    46801 acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttt
    46861 cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct cataatcata
    46921 gaaggttccg gacggttgaa tacgtattga cggtaaaatt tcttcaccga gtatcgcaac
    46981 cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt gctgaaatgc
    47041 caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc ccacactgga
    47101 accttcgcgg ctcggcttaa cgataaccgg caaacccaga gcagaaattt ctgctaactg
    47161 cttatcgctc aggccttttt caaactctgc gcgggttaac gctacccacg gcgcgaccgg
    47221 taaaccggca ccttgccaca gaagtttgct gcgtagttta tctattgaaa gcgcagatgc
    47281 catcactccg ctgccggtat aaggcaagcc catcagctcg agcatgccct gcagcgtacc
    47341 atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca tcgacttcag
    47401 ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca tcaataccgc cttcacgcag
    47461 cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg aagtcccgcc
    47521 caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt
    47581 tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga acgagaatca
    47641 ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgcaggg tccggcacca
    47701 gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc
    47761 ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta tcaacctgcg
    47821 tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa
    47881 acagcattac caggttttta tccggccagc ctgcgcgcgc cgctttaatg gtggcgtcaa
    47941 cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt ttaccattca
    48001 ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc tggaagctct
    48061 ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag
    48121 ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc atcggctctt
    48181 tgtcctgacg cagcagcgta aagtgcccct gcgggccaat ctgctgataa tcttctacac
    48241 gcacgtcggc atcttcgctg aagccgtaag ttgtggtctg acgtcccaca cgcggtaaca
    48301 attcgcggat caccggatca tcaacacaca tcaccgcacg accgtaaaaa ggcaggttgt
    48361 gcagaaaatt aataaaggtc tgttttaaat tctcaaagtc gccctggtag gtatccatgt
    48421 ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg aacgatgcat
    48481 cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca tgaacccccg
    48541 ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc
    48601 tggaaaccat cgcggtagtt gtcgttttgc cgtgcgttcc tgcaatggcg atgccatgac
    48661 gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata cgcgcttcat
    48721 gagctgcgac aatttccggg ttgtcggcag aaatggcgct ggaaacaacg accacgctgg
    48781 catcacgtac gttttccggg cgatggttga aataaatcgt cgcacccaga ttcagtaact
    48841 gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct tcattggcca
    48901 gaacttcggc aataccgccc ataccggcac caccaatgcc gacaaaatgt atgtgccgaa
    48961 cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta ttcattcttt
    49021 acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt acgcccgggc
    49081 agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc
    49141 gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt tggcgacagc
    49201 atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt ccagcggtag
    49261 cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc
    49321 tgccgcggcg atttcactca cagttaaagc accggagcgg caaacgacga catccgccca
    49381 cgcatacgcc gccgccatat catcaataaa ttcagtcact ttatgctgcg gttgccccgc
    49441 ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat gccagatagt
    49501 gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc
    49561 ctgggaacca cccactacca acacacgaac cggaccttca cgtccagcca aacgttgctg
    49621 cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt ccgcattagg
    49681 gaaagcgcct ggaaacgcct gcatcacttt ggtagcaatc ttcgccagcc atttattggt
    49741 taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg accacgcggc
    49801 cagaccacct ggacctgata catagccgcc cataccgagt accacgtcag gtttgtacgc
    49861 tttcataatc gcccgcgcct gacgccaggc gttgaagata cgcagcggcg cagctatcag
    49921 tgcttttata ccttttccac gcaaaccaga gatacgaatg aaatcaattt cgatgccatg
    49981 ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc
    50041 ctgagccatt agatagtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc
    50101 tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac aaacgcctgc
    50161 gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata
    50221 atcagtaagc tcgaaccacc gtaactgatt agcggcaatg tcagaccttt ggtcggtaac
    50281 atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat gccaatagaa
    50341 caggcgagaa aaccggaaaa acggtggtca atttctaatg ctttgcggcc aatcgacatc
    50401 gcacgaaaag cgacgaagaa taccattaaa agtgctagta ccacaccgac ataacctaat
    50461 tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt
    50521 tgtaccgaat tacctaaacc ttgcccccaa agttcgccgc gaccaaacgc catcagcgat
    50581 tgcgttaact gatagccgct gccaaaggga tcttcccacg ggttccagaa tgcggtaaca
    50641 cggcggatac ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat gcccataccg
    50701 ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc
    50761 acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc
    50821 agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta
    50881 cgcaccagat agttggcgat atagcaaaac aacgacagtt ttgtcagctc cgcaggctgg
    50941 atacgcagca aaccgagatc gatccaacgc gatgcccctt taacagcgct acctactacc
    51001 agcacgatca tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag
    51061 aactccatcg gcagacgcag cgtaatgatc gccagaataa acgccaaaat cagatagaca
    51121 ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac
    51181 gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg
    51241 cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcccttt
    51301 agcgccgtgg agatccagac caagatactg aatcctggca ggcgcggcat tttcaggcga
    51361 gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt
    51421 gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagcaga accatatcgc
    51481 ccggctgaac acgcggagcc agcaagcgca tagcctgttc catagtttcg gtttgttctg
    51541 ccacttccgg gcgtagcgcc gccagctgcg cgccgtcacg accgaaacaa tacagacgta
    51601 cgttatcgcc attcaggtaa cgcgccagtg ggctaaagtc cgccgattta ccatcgccgc
    51661 ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt cagcgccgct tccgtactgc
    51721 cgacgttggt cgcttttgaa tcgttaatcc agcgcacacc gttatgctcc agcaccactt
    51781 caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggta
    51841 accctgcggc atctgccagc gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg
    51901 aaagtttcat ctctttcaca ttcagcactt tctcgccttt aacccgcagc caggtttcgc
    51961 cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg cagcgttcat
    52021 ccgcaccgcg aatcggcatt gttaaggcat catcagcatt aaccacgcaa actttcgcgt
    52081 tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa accaaacggg tagcgatcca
    52141 tatgatcttc agtcacgttc agaatggtcg ctgctaccgc ctgtaagctg gaggtggttt
    52201 ccagctggaa gctcgacagt tccagcacgt acagttcaca ctcatcatcc agtagcatca
    52261 acgcaggcag gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt
    52321 cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg
    52381 cttgtgcttc gcgacaaaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg
    52441 cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc agatctgccg
    52501 ccatcagcca ttcatcattc agactgcccg tgtggcgttc tacggcttcg ggtaatttat
    52561 ccaggctagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga
    52621 aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacaaca tttttacctt
    52681 ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga ccagcatcag
    52741 cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca gttcatagtg
    52801 gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg
    52861 caggatgaca gaaagcgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc
    52921 ctgacgtagc agtacggcga taatgcctaa cgcaccacct aacgccagcg aaccgacatc
    52981 gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagtc ctgccccgac
    53041 tatcgcggta cagacaataa ccagttcccc ggcgtgtcgc agatacggta tgtgtaaata
    53101 gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa
    53161 tacggtcggc ataattgcca gaccgtcaag accatcggtc aggtttaccg cgttgccagt
    53221 acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagttgcg gcatcacatc
    53281 tttaaagaat gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa ggtacaaagc
    53341 gaaagcaaca cccagcgcaa tgaccgacat ccagaaatat ttccaacgag cgatcaaccc
    53401 tttggtatct ttacgcacca ctttgcgata atcatcaaca aagccaataa caccgtaacc
    53461 taccagcacc accaacacgc accagacgta cggattggac gggtaagccc acagcagcac
    53521 ggagatcaca atcgctgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct
    53581 gaagtgtgat tcaggaccgt cgttacgcac tacctgacca aaggaaagtt tttgcaaatg
    53641 agcaatcatg cgcgggccca tccacaatga gatgaacagc gcggtcagca ggctgacgat
    53701 ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc
    53761 ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggca
    53821 gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta
    53881 agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc caacgccgct ggcggtgctg
    53941 atagcatggc tttgtttacc catgcttaac acgcggtcaa taccagcagc ttttgccgcc
    54001 tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc
    54061 accagcacgc ggtagcccgg catttcagcc agtacctgaa cagctgcggt cattgaaccg
    54121 acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttg gatggggaac
    54181 agacggccag gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg
    54241 cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc
    54301 aacggcagca gaacatcgac gctaccggtt ggggtttgta gggtaaattc cataccgtgc
    54361 gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc
    54421 cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc
    54481 ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca
    54541 cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgccgct
    54601 tccgggcgag tcagactcac agtccaggca atctcgccct gatggttcgc gccaagttca
    54661 ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac accgatgtcg
    54721 ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc
    54781 atctctttaa cggaggtttt gccggaggac cccgtcagag caaccacgcg cgccggaact
    54841 tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt gacgattaac
    54901 tgcggcaggt cgatgtccag cggacggcta accagtagtg cgcctgcgcc gccagctttc
    54961 gcctggtcgg caaaatcgtg ggcatcaaaa cgttcacctt tcagggcaac aaacaggcag
    55021 cccggcgtca gttttcgggt atcagtggtt acagcatcaa gggtgatatc tgcaccttgc
    55081 agttcaccgt tgagaatatc ggtaagttgg ctaagggtta cgctaatcat gcaatccccc
    55141 ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct
    55201 ggtaatcctc atggcctttg cccgcgacca gtaccacatc attctcttta gcctgcataa
    55261 cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca
    55321 ttcccgccag aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg
    55381 tcaccaccgc cacgtcagca aactcttcgg caattgcgcc catcagtgga cgcttacctt
    55441 tatcgcgatc gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg
    55501 ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg
    55561 gtttgcctgg cgcagtgaac acttccatac gtccgcaaac cggttgcaga cgcgcggcgg
    55621 ttttcagcag atcagccagt ggatagccca gcgccagcag tgttgccagc gcgagcaaca
    55681 ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccagcttg
    55741 aactaaagcg aatggtcgcg ccgctgtcgt gatagttcac ttcggtcgct ttcaaccagc
    55801 gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaaccgcg tccggcagtt
    55861 ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga ccgcaatgat
    55921 gttcagaata aagcagccat ttcgcagctt cgtagtgttc catatcacca tgataatcaa
    55981 ggtgatcgcg gcttaagttg gtaaagaccg acgccggaaa tttcaatgcc gccacacggt
    56041 gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca
    56101 gccccgccag ctcatgctga acatcaactg ccgaaccggt agtattttct gtcgggatca
    56161 ctttccccag cagaccatta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt
    56221 ggctccactg cgccagaagc tgggtcgtcg tggttttgcc gttggtgccc gttacgccta
    56281 cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct
    56341 cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg
    56401 tcgcctcatc tttcgcctct gcaataatgg cagccacacc ttgcgctatc gcctgcggga
    56461 tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg cccgccgcag
    56521 ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct ggcacccacg
    56581 gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg attaatcaca
    56641 aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt acgcaatacg
    56701 ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc
    56761 tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct
    56821 gcggtataag caatatattt attgatgtag cgaccgtccg gcccgacctt tttcgcggta
    56881 ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc gccgcctggt
    56941 agcgccacgc tttccatcat atgcaccacg gtgcgaacaa tggattccgg gaagacacgt
    57001 tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat gccgtagctg
    57061 ccgatagttg cgtagactcg cgctaactgt aacggtgtta ccattagccc gtagccgaaa
    57121 gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa gccactgcgt
    57181 tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta agtatctact
    57241 aacgctgagg acggcatcgc taacgccagc ttggaaacac cgacgttact cgacttctgt
    57301 aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta
    57361 attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg
    57421 gtcatcacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt gatggtacgg
    57481 ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga cgggctgtta
    57541 gccatcgcca gcacttcacc tgtgttgaca tccaccagca cggcgctacc agattcagcc
    57601 ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag gcgttcatca
    57661 atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg
    57721 cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg
    57781 aaactcttct caacgccctc aatcccttga ctatcgacgt tagtaaagcc gatgaggtga
    57841 gcagtcactt cgccggacgg atagtaacgg cgagactctt cacgcagatg aatccccggc
    57901 agtttcagtt ttttgatgta gtccgccatg tcagagttta cctgacgcgc cagataaata
    57961 aagcgccctt tcgggttggc gttaatgcgg gcagaaagct gatccagcgg aatattgagc
    58021 gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc atgcacttct
    58081 ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg
    58141 tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc
    58201 tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa
    58261 gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat aaagttggca
    58321 tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat
    58381 ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt
    58441 tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg
    58501 ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg ctgagcggtc
    58561 agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg
    58621 aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat
    58681 gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg
    58741 ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca cgagggttct
    58801 cagccacctc ttcttcgccc ggcattaact tgcctagtgc tcgcagctga cggccaccca
    58861 gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga ccgcggctgt
    58921 tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa
    58981 gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca
    59041 gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact
    59101 tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg
    59161 gctgttcgcg gttacgctcg acaatagcgc gggcaatgcg tttggcaaaa cgctcttcac
    59221 cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg
    59281 ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa
    59341 agccgcgttc agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt
    59401 cgatcttgcc aataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt
    59461 ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc
    59521 gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac
    59581 caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa
    59641 cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag
    59701 tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat atcttccttg
    59761 acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc
    59821 atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct
    59881 gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca
    59941 accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg
    60001 gggtaaagca gcaggcacgg gtgatgaatg tcaatggcgc aaaccatttg accggcagcg
    60061 ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt gctgtcgaga
    60121 ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac
    60181 aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg
    60241 cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc
    60301 aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca
    60361 ctgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga
    60421 gcggcatttt aggacatatc ttccccggtt aacagtcctt gttacgtctg tgtggcgctt
    60481 gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg ttaaatcggc aattcatcgc
    60541 cgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc
    60601 agctggtgag ttaattaacg ggcgggttta aaaccattta tcaataatag tcaggaatag
    60661 tcttatttac tttaaggata ttgat
//
LOCUS       DADWTH010000012        59685 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.012, whole genome shotgun sequence.
ACCESSION   DADWTH010000012 DADWTH010000000
VERSION     DADWTH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.012"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(409..1575)
                     /locus_tag="JNY02_000745"
     CDS             complement(409..1575)
                     /locus_tag="JNY02_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HBB9432395.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(1597..2136)
                     /locus_tag="JNY02_000746"
     CDS             complement(1597..2136)
                     /locus_tag="JNY02_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432396.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(2392..2736)
                     /gene="tdcR"
                     /locus_tag="JNY02_000747"
     CDS             complement(2392..2736)
                     /gene="tdcR"
                     /locus_tag="JNY02_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBB9432397.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            2925..3863
                     /gene="tdcA"
                     /locus_tag="JNY02_000748"
     CDS             2925..3863
                     /gene="tdcA"
                     /locus_tag="JNY02_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBB9432398.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            3962..4951
                     /gene="tdcB"
                     /locus_tag="JNY02_000749"
     CDS             3962..4951
                     /gene="tdcB"
                     /locus_tag="JNY02_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBB9432399.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            4973..6304
                     /gene="tdcC"
                     /locus_tag="JNY02_000750"
     CDS             4973..6304
                     /gene="tdcC"
                     /locus_tag="JNY02_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBB9432400.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            6330..7538
                     /gene="tdcD"
                     /locus_tag="JNY02_000751"
     CDS             6330..7538
                     /gene="tdcD"
                     /locus_tag="JNY02_000751"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBB9432401.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWIASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            7572..9865
                     /gene="tdcE"
                     /locus_tag="JNY02_000752"
                     /pseudo
     CDS             7572..9865
                     /gene="tdcE"
                     /locus_tag="JNY02_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            9879..10268
                     /locus_tag="JNY02_000753"
     CDS             9879..10268
                     /locus_tag="JNY02_000753"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBB9432402.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            10340..11704
                     /gene="tdcG"
                     /locus_tag="JNY02_000754"
     CDS             10340..11704
                     /gene="tdcG"
                     /locus_tag="JNY02_000754"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBB9432403.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCD"
     gene            11979..13256
                     /locus_tag="JNY02_000755"
     CDS             11979..13256
                     /locus_tag="JNY02_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432404.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILR
                     RKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLV
                     YKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            13284..14576
                     /locus_tag="JNY02_000756"
     CDS             13284..14576
                     /locus_tag="JNY02_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBB9432405.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14788..14952)
                     /locus_tag="JNY02_000757"
     CDS             complement(14788..14952)
                     /locus_tag="JNY02_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432406.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(14975..15676)
                     /locus_tag="JNY02_000758"
     CDS             complement(14975..15676)
                     /locus_tag="JNY02_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBB9432407.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            15781..16677
                     /gene="yhaJ"
                     /locus_tag="JNY02_000759"
     CDS             15781..16677
                     /gene="yhaJ"
                     /locus_tag="JNY02_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBB9432408.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGQLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            complement(16884..17252)
                     /locus_tag="JNY02_000760"
     CDS             complement(16884..17252)
                     /locus_tag="JNY02_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBB9432409.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(17545..18531)
                     /gene="yqjG"
                     /locus_tag="JNY02_000761"
     CDS             complement(17545..18531)
                     /gene="yqjG"
                     /locus_tag="JNY02_000761"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBB9432410.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(18601..18993)
                     /locus_tag="JNY02_000762"
     CDS             complement(18601..18993)
                     /locus_tag="JNY02_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBB9432411.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFSFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(19179..19478)
                     /locus_tag="JNY02_000763"
     CDS             complement(19179..19478)
                     /locus_tag="JNY02_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBB9432412.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(19468..19872)
                     /locus_tag="JNY02_000764"
     CDS             complement(19468..19872)
                     /locus_tag="JNY02_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBB9432413.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(19875..20180)
                     /locus_tag="JNY02_000765"
     CDS             complement(19875..20180)
                     /locus_tag="JNY02_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBB9432414.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(20218..20572)
                     /locus_tag="JNY02_000766"
                     /pseudo
     CDS             complement(20218..20572)
                     /locus_tag="JNY02_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            complement(20719..21102)
                     /gene="mzrA"
                     /locus_tag="JNY02_000767"
     CDS             complement(20719..21102)
                     /gene="mzrA"
                     /locus_tag="JNY02_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBB9432415.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(21106..21768)
                     /locus_tag="JNY02_000768"
     CDS             complement(21106..21768)
                     /locus_tag="JNY02_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBB9432416.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(22113..22889)
                     /gene="exuR"
                     /locus_tag="JNY02_000769"
     CDS             complement(22113..22889)
                     /gene="exuR"
                     /locus_tag="JNY02_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBB9432417.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            23185..23901
                     /locus_tag="JNY02_000770"
     CDS             23185..23901
                     /locus_tag="JNY02_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBB9432418.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            23931..24431
                     /locus_tag="JNY02_000771"
     CDS             23931..24431
                     /locus_tag="JNY02_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9432419.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            24523..27032
                     /locus_tag="JNY02_000772"
                     /pseudo
     CDS             24523..27032
                     /locus_tag="JNY02_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
     gene            27029..28117
                     /locus_tag="JNY02_000773"
     CDS             27029..28117
                     /locus_tag="JNY02_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBB9432420.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(28205..29503)
                     /gene="exuT"
                     /locus_tag="JNY02_000774"
     CDS             complement(28205..29503)
                     /gene="exuT"
                     /locus_tag="JNY02_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBB9432421.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            29986..31398
                     /gene="uxaC"
                     /locus_tag="JNY02_000775"
     CDS             29986..31398
                     /gene="uxaC"
                     /locus_tag="JNY02_000775"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBB9432422.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSSRGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            31413..32900
                     /gene="uxaA"
                     /locus_tag="JNY02_000776"
     CDS             31413..32900
                     /gene="uxaA"
                     /locus_tag="JNY02_000776"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBB9432423.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            32983..33534
                     /locus_tag="JNY02_000777"
     CDS             32983..33534
                     /locus_tag="JNY02_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBB9432424.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(33539..34783)
                     /gene="sstT"
                     /locus_tag="JNY02_000778"
     CDS             complement(33539..34783)
                     /gene="sstT"
                     /locus_tag="JNY02_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBB9432425.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(35379..36344)
                     /gene="alx"
                     /locus_tag="JNY02_000779"
     CDS             complement(35379..36344)
                     /gene="alx"
                     /locus_tag="JNY02_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBB9432426.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(36615..37613)
                     /locus_tag="JNY02_000780"
     CDS             complement(36615..37613)
                     /locus_tag="JNY02_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB9432427.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(37692..38384)
                     /locus_tag="JNY02_000781"
     CDS             complement(37692..38384)
                     /locus_tag="JNY02_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBB9432428.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(38461..38964)
                     /locus_tag="JNY02_000782"
     CDS             complement(38461..38964)
                     /locus_tag="JNY02_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBB9432429.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            39049..40185
                     /gene="rlmG"
                     /locus_tag="JNY02_000783"
     CDS             39049..40185
                     /gene="rlmG"
                     /locus_tag="JNY02_000783"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBB9432430.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            40470..40784
                     /gene="higB"
                     /locus_tag="JNY02_000784"
     CDS             40470..40784
                     /gene="higB"
                     /locus_tag="JNY02_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBB9432431.1"
                     /translation="MHLITQKALKDAAEKHPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            40781..41197
                     /gene="higA"
                     /locus_tag="JNY02_000785"
     CDS             40781..41197
                     /gene="higA"
                     /locus_tag="JNY02_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBB9432432.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(41242..43260)
                     /gene="fadH"
                     /locus_tag="JNY02_000786"
     CDS             complement(41242..43260)
                     /gene="fadH"
                     /locus_tag="JNY02_000786"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBB9432433.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIPPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLQLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(43486..45837)
                     /gene="ygjK"
                     /locus_tag="JNY02_000787"
     CDS             complement(43486..45837)
                     /gene="ygjK"
                     /locus_tag="JNY02_000787"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBB9432434.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(45854..46273)
                     /locus_tag="JNY02_000788"
     CDS             complement(45854..46273)
                     /locus_tag="JNY02_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432435.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(46251..46604)
                     /locus_tag="JNY02_000789"
     CDS             complement(46251..46604)
                     /locus_tag="JNY02_000789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432436.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(46605..46925)
                     /locus_tag="JNY02_000790"
     CDS             complement(46605..46925)
                     /locus_tag="JNY02_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432437.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGMEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(47059..48492)
                     /locus_tag="JNY02_000791"
     CDS             complement(47059..48492)
                     /locus_tag="JNY02_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB9432438.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(48555..49004)
                     /locus_tag="JNY02_000792"
     CDS             complement(48555..49004)
                     /locus_tag="JNY02_000792"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBB9432439.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(49001..52087)
                     /gene="ebgA"
                     /locus_tag="JNY02_000793"
     CDS             complement(49001..52087)
                     /gene="ebgA"
                     /locus_tag="JNY02_000793"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBB9432440.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAYYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIINRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(52269..53252)
                     /gene="ebgR"
                     /locus_tag="JNY02_000794"
     CDS             complement(52269..53252)
                     /gene="ebgR"
                     /locus_tag="JNY02_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBB9432441.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELNN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            53471..53802
                     /locus_tag="JNY02_000795"
                     /pseudo
     CDS             53471..53802
                     /locus_tag="JNY02_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(53844..55175)
                     /gene="ygjG"
                     /locus_tag="JNY02_000796"
     CDS             complement(53844..55175)
                     /gene="ygjG"
                     /locus_tag="JNY02_000796"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBB9432442.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEM
                     FRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            55592..57112
                     /gene="aer"
                     /locus_tag="JNY02_000797"
     CDS             55592..57112
                     /gene="aer"
                     /locus_tag="JNY02_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBB9432443.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMFTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(57266..57889)
                     /gene="nfeR"
                     /locus_tag="JNY02_000798"
                     /gene_synonym="yqjI"
     CDS             complement(57266..57889)
                     /gene="nfeR"
                     /locus_tag="JNY02_000798"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBB9432444.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            58166..58912
                     /gene="nfeF"
                     /locus_tag="JNY02_000799"
                     /pseudo
     CDS             58166..58912
                     /gene="nfeF"
                     /locus_tag="JNY02_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(58966..59041)
                     /locus_tag="JNY02_000800"
     tRNA            complement(58966..59041)
                     /locus_tag="JNY02_000800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59006..59008),aa:Ile,seq:cat)
     gene            59166..59672
                     /gene="mug"
                     /locus_tag="JNY02_000801"
     CDS             59166..59672
                     /gene="mug"
                     /locus_tag="JNY02_000801"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBB9432445.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT        14814 a        14596 c        15314 g        14961 t
ORIGIN      
        1 ggtcagtttc acctgatata cgtaaaaacc cgcttcggcg ggtttttgct tttggagggg
       61 cagaaagatg aatgactgtc cacgacacta tacccaaaag aaagcggctt atcggtcagt
      121 ttcacctgat gtacgtaata aaccgttccg gcgggtttca gattgttgag tgcgctttat
      181 tcatgccgga tgctgcgtga acgccttatc ctgcctacaa aagcacgcaa attcaatata
      241 ttgcagagat ggcgtaggcc tgataagcgt agcgcatcag gcatttttgc ggttgccatc
      301 agtttcaaac ccgcgtcgat aagtttttgc ttttggtgca cagggtaaga tgaatatcta
      361 cggtattaat aatagacttt gtaagaagtt attaagtcta tattgcattt aaaaggtcgt
      421 aattatacgt atagtcctct ctggtaaaat attgattctc tgagttatat gcttcatatg
      481 tgttatattc cccgacctca aagccattgt ccgtagaatt ttcgtctttc tcgagggtat
      541 taactcttct gagctagagt cttctatgtt taaatccata gtataattaa tttgggggac
      601 gaccagataa gcattttctt tgatattttc ataatcttca atgattgtat cgatgtaatc
      661 atatattttt tttgatgaga attctttcat ctctggtaat ttaattctgt tcaagcaatt
      721 gttgattttc gatgagcttt tatatatttc ttttgttaat tcattttcta atttatctgt
      781 aagagttgcc cttggtatag attttataat tgcaaatctc ataattttta attgttcttc
      841 ggtaatgtct ccaagctcat ttaaacaact ttcgacaaac ttgatttttt cttcagaata
      901 catatcgcta atgcttatta aaacaggcgt tatttttagt aaataatccc aggagattgt
      961 ggcgttttgt gttgaactaa caaaattaac ctgaaacatg ttggcattag taaaatttgt
     1021 tgaatccaat ttaaaatgat ttagaatagt attagccata tgtttatctg tgaaatcata
     1081 ataatctaaa tcgacattac gtaagtccag agaggataga tcaactatat ctccagtccc
     1141 acaactaaag attacgttca acctcctgat ttcattgtaa atatctttta atgggcaatt
     1201 aacattgata ttgcctgtag atatttcctg gttttttaac ggttcattaa tggtaagttc
     1261 gcaggtattg tccgatcgtt gaataatttg ggcgttataa cctaaaaaat ccagacgaag
     1321 agacgagtca ttataaagaa ttacattttg taagtacgct aattcttttt gaaaatcacc
     1381 agtgataatg cattttccac ttggttgtaa cgcgttaagt aaagtgacca tttgaataga
     1441 ggtctgtttt agtttttcta taccgggtgc tagtgcttcg aggttatttc ctatggggct
     1501 ttcaggaagg tcattcattt ttcgacggac taaatcacta ctaatatcat taccaattga
     1561 tgaaacaggg aacattattt tatcctttat aaataatcaa taaaaatcat tattaaaatg
     1621 atcgggtgaa aaaaagagaa tttgccaaca atctgaagta atgcaggttt tattaaagtg
     1681 cctagggggt gttaagatgg cttctttttg ggtgaacttt gcgcaatact tagaaatcgt
     1741 ctccttaata cattccagct cttttttatt gacggctgag caagtacatt gttcaccaaa
     1801 aggtgcaaaa gtgcctacat ctaatgtttg tatgatataa tcatcatgtt ctcctgtaga
     1861 aatatctatg atgaatggac tcatattttc cggttggatt cggatcatga cagaaggaac
     1921 atcgccttga tagaaattca atgcagtaat tgcagataat actctccgca gttcattact
     1981 tatactgtaa attttattaa cgtcattggt ggagacaatt tctgcaaact ttctttttac
     2041 cgtccaataa tcaatatttc tattgtgatt gttaaccact gcgtgcattg gcgggattga
     2101 agggtgaaaa atatgtgcaa ttgggaagcc tttcattgat gcctgccttg tcgtcatgac
     2161 aaacaataat agatgcttat tttcctcgca cgacacttac gcactatcaa aaaacgctca
     2221 tttacccggc gttacagtaa acgaagcaac attctgtgtc ctacgctgat gtaaagcaca
     2281 gtgaaaatcc cccttgttga cttcgtctga ccttatcgcg gcgaccagca attattcgtt
     2341 ttgtgatcct caacacaaaa tgctattcgt cgcctatgag gggagaagaa tttatggaaa
     2401 acgattgcat tcacaaaatg acgtcctgtc agaagagaaa aagttgacgt taatcaattg
     2461 ttcgagatga tcaatacctg tattcgaaat tttaaaatat tgataaaata aatgtttgtt
     2521 ctccaacaaa tagcgtgaaa aaatgtgacg tgccgcagta tttatcgctg aaaatggttt
     2581 atttataata aaatttgaga acttgctcat taaatttaac tcaaattttg cctggtaatt
     2641 atccggtaat tgcttgaaat atggtctcag cccctttttt gtattaacca cataacgaat
     2701 gatgttatcg ccataaaata tggttatccc cgtcattttt tttgatagaa atcagggttt
     2761 atgctgattt ttatgcttta acttgttgat atttaaaggt atttaattgt aataacgata
     2821 ccttggaaag taatgaaagt taatttgtga gtggtcgcac atatcctgtt tatttcattt
     2881 tgatacactt catgccgtca atgaggtaat taacgtaggt cgttatgagc actattcttc
     2941 ttccgaaaac gcagcacctg gtagtctttc aggaagtcat tagaagtggc tctatcggct
     3001 cggctgcaaa agaattaggg ttaactcaac cggccgtcag taaaatcatt aacgatattg
     3061 aagattattt tggtgtggaa ttagtggtgc ggaaaaatac cggtgtaaca ttaacacctg
     3121 ccggtcaatt gttactctcc cgttccgaat ccattacccg tgaaatgaaa aatatggtta
     3181 atgagataag cggtatgtct tctgaggcgg tggtggaagt ctcatttggt tttccttcat
     3241 tgattggttt tacttttatg tcagggatga tcaacaagtt caaagaggtg ttcccgaaag
     3301 cgcaggtttc tatgtatgaa gcgcaactgt cttcgttctt accggcaatc cgcgacggtc
     3361 gactggattt tgcgattggt acgttaagtg cagaaatgaa gcttcaggat ttacatgttg
     3421 agccgctgtt cgagtccgag tttgtgctgg tagccagtaa gtcccgaaca tgcaccggca
     3481 ccaccacgct ggagtcgttg aagaacgaac agtgggtgtt gccacaaact aatatggggt
     3541 actacagcga actgcttact acgttacaaa gaaatggcat cagtattgaa aacatcgtta
     3601 aaaccgactc agtcgtgaca atttataatc ttgttctcaa tgctgatttc ttaactgtaa
     3661 ttccttgtga tatgacgtca ccttttggtt ctaatcaatt tattactatt ccggttgaag
     3721 aaacattacc tgtggcacaa tatgccgcgg tatggtcgaa aaattatcgt attaaaaaag
     3781 cagcatcggt tttggtggaa ttagccaaag agtattcatc ttataatggg tgtagacgaa
     3841 ggcaattaat agaagttggt tagttatttg tttttattta aacataaata atccacctgt
     3901 ctgttttgca ggtgtcggtt acggttacct acatatttaa ttcaggcgaa gaggttttat
     3961 aatgcatatt acatacgatc tgccggttgc tattgatgat attattgaag cgaaacaacg
     4021 actggctggg cgaatttata aaacaggcat gcctcgctcc aactatttta gtgaacgttg
     4081 caaaggtgaa atattcctga agtttgaaaa tatgcagcgt acgggttcat ttaaaattcg
     4141 tggcgcattt aataaattaa gttcactgac cgatgcggaa aaacgcaaag gcgtggtggc
     4201 ctgttctgcg ggcaaccatg cgcaaggggt ttcgctctcc tgcgcgatgc tgggtatcga
     4261 cggtaaagtg gtgatgccaa aaggtgcgcc aaaatccaaa gtagcggcaa cgtgcgacta
     4321 ctccgcagaa gtcgttctgc atggtgataa cttcaacgac actatcgcta aagtgagcga
     4381 aattgtcgaa atggaaggtc gtatttttat cccaccttac gatgatccga aagtgattgc
     4441 tggccaggga acgattggtc tggaaattat ggaagatctc tatgatgtcg ataacgtgat
     4501 tgtgccaatt ggtggtggtg gtttaattgc tggtattgcg gtggcaatta aatctattaa
     4561 cccgaccatt cgtgttattg gcgtacagtc agaaaacgtt cacggcatgg cggcttcttt
     4621 ccactccgga gaaataacca cgcaccgaac taccggcacc ctggcggatg gttgtgatgt
     4681 ctcccgcccg ggtaatttaa cttacgaaat cgttcgtgaa ttagtcgatg acatcgtgct
     4741 ggtcagcgaa gacgaaatca gaaacagtat gattgcctta attcagcgca ataaagtcgt
     4801 caccgaaggc gcaggcgctc tggcatgtgc tgcattatta agcggtaaat tagaccaata
     4861 tattcaaaac agaaaaaccg tcagtattat ttccggcggc aatatcgatc tttctcgcgt
     4921 ctctcaaatc accggttttg ttgacgctta attaattcgt tgaggatagg atatgagtac
     4981 ttcagatagc attgtatcca gccagacgaa acaatcgtcc tggcgtaaat cagataccac
     5041 atggacgtta ggcttgtttg gtacggcaat cggcgccggg gtgctgttct tccctatccg
     5101 cgcaggtttt ggcggactga tcccgattct tctgatgttg gtattggcat accccatcgc
     5161 attttattgc caccgggcgc tggcgcgtct gtgtctttct ggctctaacc cttccggcaa
     5221 cattacggaa acggtggaag agcattttgg taaaactggc ggcgtggtta tcacgttcct
     5281 gtacttcttc gcgatttgcc cactgctgtg gatttatggc gttactatta ccaatacctt
     5341 tatgacgttc tgggaaaacc agctcggatt tgcaccgctg aatcgcggct ttgtggcgct
     5401 gttcctgttg ctgctgatgg ctttcgtcat ctggtttggt aaggatctga tggttaaagt
     5461 gatgagctac ctggtatggc cgtttatcgc cagcctggtg ctgatttctc tgtcgctgat
     5521 cccttactgg aactctgcag ttatcgacca ggttgacctc ggttcgctgt cgttaaccgg
     5581 tcatgacggt atcctgatca ctgtctggct ggggatttcc atcatggttt tctcctttaa
     5641 cttctcgcca atcgtctctt ccttcgtggt ttctaaacgt gaagagtatg agaaagactt
     5701 cggtcgcgac ttcaccgaac gtaaatgttc ccaaatcatt tctcgtgcca gcatgctgat
     5761 ggttgcagtg gtgatgttct ttgcctttag ctgcctgttt actctgtctc cggccaacat
     5821 ggcggaagcc aaagtgcaga atattccagt gctttcttat ctggctaacc actttgcgtc
     5881 catgaccggt acgaaaacaa cgttcgcgat tacactggaa tatgcggctt ccatcatcgc
     5941 actcgtggct atcttcaaat ctttcttcgg tcactatctg gggacgctgg aaggcttgaa
     6001 tggtctgatt ctgaagttcg gttataaagg tgacaaaacc aaagtgtcgc tgggtaaact
     6061 gaatactctc agcatgatct tcatcatggg ctccacctgg gttgttgcct acgccaaccc
     6121 gaacatcctc gacctgattg aagccatggg cgcaccgatt atcgcatccc tgctgtgcct
     6181 gttgccgatg tatgctatcc gtaaagcgcc gtctctggcg aaataccgtg gtcgtctgga
     6241 taacgtgttt gttaccgtga ttggtctgct gaccatcctg aacatcgtat acaaactgtt
     6301 ttaatccgta actcaggatg agaaaagaga tgaatgaatt tccggttgtt ttggttatta
     6361 actgtggttc gtcttcgatt aagttttccg tactcgatgc cagcgactgt gaagtattaa
     6421 tgtcaggtat tgccgacggt attaactcgg aaaatgcatt cttatccgta aatgggggag
     6481 agccagcacc gctggctctc cacagctacg aaggtgcatt gaaggcaatt gcatttgaac
     6541 tggaaaaacg gaatttaaat gacagcgtgg ccttaattgg ccaccgcatc gctcacggcg
     6601 gcagtatttt taccgagtcc gccattatta ccgatgaagt cattgataat atccgtcgcg
     6661 tttctccact ggcacccctg cataattacg caaatttaag tggtattgaa tcggcgcagc
     6721 aattatttcc gggcgtaact caggtggccg tatttgatac cagtttccac cagacgatgg
     6781 ctccggaagc ttatttatac ggcctgccgt ggaaatatta tgaagagtta ggtgtacgcc
     6841 gttatggttt ccacggcacg tcgcaccgct atgtttccca gcgcgcacat tcgctgctga
     6901 atctggcgga agatgactcc ggcctggttg tggcgcatct tggcaatggc gcgtcaatct
     6961 gcgcagttcg caacggtcag agtgttgaca cctcaatggg aatgacgccg ctggaaggct
     7021 tgatgatggg tacccgcagt ggcgatgtcg actttggtgc gatgtcctgg atcgccagcc
     7081 aaaccaacca gagcctgggt gacctggaac gcgtagtgaa taaagagtcg ggattattag
     7141 gtatttccgg tctttcttcg gatttacgtg ttctggaaaa agcctggcat gaaggtcacg
     7201 aacgcgcgca actggcaatt aaaacctttg ttcaccgaat tgcccgtcat attgccggac
     7261 atgcagcttc attacgtcgg ctggatggaa ttatattcac cggcggaata ggagagaatt
     7321 caagtttaat tcgtcgtctg gtcatggaac atttggctgt attaggcgta gagattgata
     7381 cagaaatgaa taatcgatct aactcttttg gcgagcgaat tgtttccagt gaaaatgcgc
     7441 atgtcatttg cgtcgttatt ccgactaacg aagaaaaaat gattgctttg gatgccattc
     7501 atttaggtaa agttaacgcg cccgcagaat ttgcataatt tagttgaagt attgtagaga
     7561 gattattttt catgaaggta gatattgata ccagcgataa gctgtacgct gacgcatggc
     7621 ttggctttaa aggtacggac tggaaaagcg aaattaatgt ccgcgatttt attcaacata
     7681 actatacacc gtatgaaggc gatgaatctt ttctcgccga agcgacgcct gccaccacgg
     7741 aattgtggga aaaagtaatg gaaggcatcc gtatcgaaaa cgcaacccac gcgccggttg
     7801 atttcgatac caatattgcc accacaatta ccgctcatga tgcgggatat attaaccagc
     7861 cgctggaaaa aattgttggc ctgcaaacgg atgcgccgtt gaaacgtgcg ctacacccgt
     7921 tcggtggcat taatatgatt aaaagttcat tccacgccta tggccgtgaa atggacagtg
     7981 aatttgaata tctgtttacc gatctgcgta aaacccataa ccagggcgta tttgatgttt
     8041 actcaccgga tatgctgcgc tgccgtaaat ctggcgtgct gaccggttta ccagatggct
     8101 atggtcgtgg gcgcattatc ggtgactatc gccgcgtagc gctgtatggc atcagttatc
     8161 tggtgcgtga acgcgaactg caatttgccg atctccagtc tcgtctggaa aaaggcgagg
     8221 atctggaagc caccatccgt ctgcgtgagg agctggcaga gcatcgtcat gcgctgttgc
     8281 agattcagga aatggcggcg aaatatggct ttgatatctc tcgcccggcg cagaatgcac
     8341 aggaagcggt gcagtggctc tacttcgctt atctggcggc agtgaaatcg caaaatggcg
     8401 gcgcgatgtc gctggggcgc acggcatcgt tcctggatat ctacattgag cgcgacttta
     8461 aagctggcgt cctcaatgag cagcaggcac aggaactgat cgatcacttc atcatgaaga
     8521 tccgtatggt acgcttcctg cgtacaccgg aatttgattc gctgttctcc ggcgacccaa
     8581 tctgggcgac ggaagtgatc ggcgggatgg ggctggacgg tcgtacgctg gtgaccaaaa
     8641 actccttccg ctatttgcac acctgcacac tatggggccg gcaccggaac ctaacctgac
     8701 cattctttgg tcggaagaat taccgattgc cttcaaaaaa tatgccgcgc aggtgtcgat
     8761 cgtcacctct tccttgcagt atgaaaatga cgatctgatg cgtactgact tcaacagcga
     8821 cgattacgcg attgcctgct gcgtcagccc aatggtgatt ggtaagcaaa tgcagttctt
     8881 tggtgcacgc gctaacctgg cgaaaacgct gctctacgca attaacggcg gggtggacga
     8941 gaagctgaag attcaggtcg ggccgaaaac agcaccgctg atggacgacg tgctggatta
     9001 cgacaaagtg atggacagcc tcgatcactt catggactgg ctggcggtgc agtacatcag
     9061 cgcgctgaat atcattcact acatgcacga caagtacagc tacgaagctt cgctgatggc
     9121 gctgcacgat cgtgatgtct atcgcactat ggcatgcggc atcgcgggcc tgtcggtggc
     9181 gacggactcc ctgtctgcca tcaaatatgc ccgcgtgaaa ccaatccgtg acgaaaacgg
     9241 cctggcggtg gactttgaaa tcgacggtga atatccgcag tacggcaaca acgacgagcg
     9301 cgtagacagc attgcctgcg acctggttga acgctttatg aagaaaatta aagcgctgcc
     9361 aacctatcgc aacgccgtcc ctacccagtc gattctgact atcacttcta acgtggtgta
     9421 cggccagaaa accggtaata cgccggacgg tcgtcgcgcc ggaacaccgt tcgcgccggg
     9481 cgctaacccg atgcatggtc gtgaccgcaa aggtgccgtg gcctcattga cgtcggtggc
     9541 gaaactgccg ttcacctacg ccaaagatgg gatctcgtac accttctcaa tcgttcctgc
     9601 ggcgctgggc aaagaagatc cagtacgtaa aaccaacctt gtcggcctgc tggatgggta
     9661 tttccaccac gaagcggatg tcgaaggcgg tcaacacctc aacgtcaacg taatgaatcg
     9721 ggaaatgctg ctggatgcca tcgagcaccc ggaaaaatat cctaacctga caatccgtgt
     9781 ctctggctac gccgtgcgct tcaacgcact gacccgtgaa cagcaacagg atgttatttc
     9841 acgtactttt acccaggcgc tctgacgccg gaggatgtat gaaaaagatt atcgaaacgc
     9901 aacgtgccct aggcgcaatc ggcccttatg ttcagggcgt tgatttaggc agcatggtct
     9961 tcacctccgg gcaaattccg gtttgcccgc agaccggtga gatcccggct gatgtgcaag
    10021 atcaggcgcg tttaagcctc gaaaacgtca aagcgatcgt ggttgctgcc gggctgagcg
    10081 tgggcgatat catcaagatg accgtgttca tcaccgatct gaatgatttt gccaccatca
    10141 atgaagtcta taagcagttc ttcgatgagc atcaggcgac ctacccgacc cggagctgtg
    10201 tgcaggttgc gcgtttgccg aaagatgtga agctggaaat tgaagctatc gcagtacgta
    10261 gtgcgtaaag cctcgtgagc gggacggtcg caaggtcgtt ccgctccact tcactgaacg
    10321 gcaatccgag ggtgtggata tgattagtgc attcgatatt ttcaaaattg ggattggtcc
    10381 ctccagttcg cataccgtgg ggccaatgaa tgccggaaaa agttttattg atcggctgga
    10441 aagtagcggc ttattaaccg cgacgagcca tattgtggtc gatctgtacg ggtcgttgtc
    10501 actgacgggc aaaggccatg ccacggatgt cgccattatc atgggactgg caggaaacag
    10561 tccgcaggat gttgtcattg atgagatccc tgcatttata gagttagtaa cgcgcagcgg
    10621 gcggctgcca gtggcatctg gtgcgcatat tgttgatttt cctgtagcaa agaacattat
    10681 cttccatccc gaaatgttgc ctcgccatga gaacggaatg cggatcactg cctggaaggg
    10741 acaggaagcg ctattaagta aaacctatta ctctgtcggc ggcgggttta ttgtcgaaga
    10801 agaacacttc ggcctgtcgc acgatgtcga aacgtccgta ccttacgatt tccactcagc
    10861 aggtgaactg ctgaaaatgt gtgattacaa cggcctgtct atatctggcc tgatgatgca
    10921 caacgagctg gcgctgcgca gcaaagcgga aattgacgca gggtttgccc gtatctggca
    10981 agtgatgcat gacggtattg aacgcgggat gaacactgaa ggcgtgctgc ctggtccgct
    11041 caatgtgccg cgtcgtgccg tagcgctgcg tcgtcagctg gtttctagcg ataacatctc
    11101 taacgatccg atgaatgtca tcgactggat caacatgtac gcgctggcgg ttagtgaaga
    11161 aaacgcagct ggcgggcgcg tggtaacggc accgactaac ggtgcgtgcg gcattattcc
    11221 ggcagtgctg gcttattacg ataagttccg tcgtccggta aacgagcggt caattgcccg
    11281 ctattttctg gcagcggggg ctattggcgc gctgtataaa atgaacgcct ccatctctgg
    11341 cgcggaggtc ggctgtcagg gggagattgg cgtggcctgt tcaatggcag cggcagggtt
    11401 aactgaacta ctgggcggca gtccggcgca ggtatgcaat gcggcggaaa tcgcgatgga
    11461 gcataacctt gggctgacct gcgatccggt tgccggacag gtacaaatcc cgtgcattga
    11521 acgtaatgcc attaatgccg tgaaagcagt aaacgccgcg cggatggcga tgcgccgcac
    11581 ctcggcaccg cgtgtttcac tcgataaagt gatcgagacg atgtatgaaa ccggcaaaga
    11641 tatgaacgat aaataccgcg aaacatcacg cggaggactg gccattaaag tggtctgcga
    11701 ctgacattgc tgtcagcttt catccttggg tgcacaaatg tgcacctttt ttttgtgatc
    11761 tgccgcaaat agagaatgaa tattctcttg cgattaattt agtagaaaat tcttatccat
    11821 ctccgttatt ttaaagtctt gattgtgaag attttctctg acctcgcgcg tatcatttct
    11881 ttattctaat aactcgcctg caaaaacgta gaccgcatat cgccttgatg cggctcggaa
    11941 aaaaataata agccctacaa aatattgagc gagaaattat ggaaattgca tcgaataaag
    12001 gcgtcattgc agacgcttcg accccggcgg gtcgtgctgg aatgagtgag agcgagtggc
    12061 gagaagcgat caaattcgac agtactgaca ccggctgggt gattatgagt atcgggatgg
    12121 cgattggcgc ggggattgtt tttctcccgg tgcaggtcgg tttgatggga ttgtgggtat
    12181 ttttgctctc atcggtgatt ggttacccgg caatgtatct gtttcagcgg ttgtttatta
    12241 atacgctggc agaatcacca gaatgtaaag attacccgag tgtcattagc ggttatttag
    12301 gtaaaaactg gggcatcctg ttaggtgcgc tctatttcgt aatgctggtg atttggatgt
    12361 tcgtctattc caccgccatc accaacgata gtgcttccta tctgcatacc ttcggcgtga
    12421 cggaagggtt gctgtcagac agtccctttt atggtctggt actgatttgc attctggtgg
    12481 cgatctcttc acgcggcgag aaattgttat tcaaaatttc gaccgacatg gtgctgacca
    12541 agctgctggt agtcgctgcg ctgggcgtgt cgatggtggg aatgtggcat ctgtataacg
    12601 tcggttcgct accgccgctg gggctgctgg tgaaaaacgc cattattacg ctgccgttta
    12661 ccctgacgtc gattctgttt atccagacgt taagcccgat ggtgatctct tatcgctcgc
    12721 gggaaaaatc aattgaagtg gcgcggcata aagcattgcg ggcaatgaat atcgcgtttg
    12781 gcattttgtt tgtcaccgtc tttttctacg ccgtgtcgtt cacgctggcg attgccgcac
    12841 agtttattag cggtgacggc gcagcgtggg tgaaagtggt cagcgtcatt ctcaatatct
    12901 ttgctgtcat gactgcattc tttggcgtct atttaggctt tcgcgaagca acgcaaggga
    12961 tcgtaatgaa catcctgcgt cgcaagatgc ctgccgagaa gattaacgaa aatcttgttc
    13021 agcgcggcat catgattttc gccattttgc tggcctggag cgccatcgta ctgaacgcac
    13081 cggtgttgag cttcacctct atctgtagcc cgattttcgg catggtaggg tgcctgatcc
    13141 cggcgtggct ggtttacaaa gtaccggcat tgcacaaata caaagggatg tctctgtacc
    13201 tgattatcgt cactggtttg ttgctttgtg tttctctgtt cctggcattt tcttgattta
    13261 cctgaaattt taaggttttt aatatgtttg attcgacttt aaatccgtta tggcagcgtt
    13321 acatcctcgc cgttcaggag gaagtaaaac cggcgctggg atgtactgaa ccgatttcac
    13381 tggcgctggc ggcggcggtt gctgcggcag aactggaagg tccggttgaa cgtgtagaag
    13441 cctgggtttc gccaaatctg atgaagaacg gtctgggcat caccgttccc ggcacgggaa
    13501 tggtggggct gccgattgcg gcggcgctgg gggcgttagg tggaaatgcc aacgccgggc
    13561 tggaagtgct gaaagacgca actgcgcagg caattgccga tgccaaagca ctgctggcgg
    13621 cggggaaagt ctctgttaag atccaggaac cttgcaatga aatcctcttc tcacgcgcca
    13681 aagtctggaa cggtgagaag tgggcgtgtg tcaccatcgt cggcgggcat accaacattg
    13741 tgcatattga gacgcacaat ggtgtggtgt ttacccagca ggcgtgtgtg gcagagggcg
    13801 agcaagagtc gccgctgacg gtgctttcca gaacgacgct ggctgagatc ctgaagttcg
    13861 tcaatgaagt cccatttgcg gcgatccgct ttattctcga ttccgcgaag ctaaattgtg
    13921 cgttatcgca ggaaggtttg agcggtaagt gggggctgca tattggcgcg acgctggaaa
    13981 aacagtgcga gcgcggtttg ctggcgaaag atctctcttc atccattgtg attcgtacca
    14041 gcgcggcatc cgatgcgcgt atgggcggcg ctacgcttcc ggctatgagt aactccggct
    14101 cgggtaacca ggggatcacc gcaacaatgc ctgtggtggt tgtagcagaa cacttcggag
    14161 cggatgatga acgactggcg cgtgcgctga tgctttctca tttgagcgca atttacatcc
    14221 ataaccagtt accgcgtttg tctgcacttt gtgccgcaac gaccgcagca atgggggccg
    14281 ccgccgggat ggcatggctg gtggatgggc gttatgaaac tatctcgatg gcgatcagca
    14341 gtatgatcgg cgatgtcagc ggcatgattt gcgatggtgc gaaggtttcg accagtgctt
    14401 cggctgcgtg gaaagcggtg ttaatggcgc tggatgatac cgccgtgacc ggcaatgaag
    14461 ggatcgtggc gcatgatgtt gagcagtcga ttgccaacct gtgtgcgtta gcaagccatt
    14521 cgatgcagca aacggatcgg cagattatcg agattatggc gagcaaggcc agataaacgc
    14581 caggttttga gtattgtgcc ggatgcgatg ctggcgcacc ttatccggcc tacggggtgc
    14641 tcaacttgtt gatgttgttg tgttttgtac ctgatgccgg atgcgatgct ggcgcatctt
    14701 atccggccta cggggtgttt aatttgttga tgttgttgtg ttttgtaggc cggataaggc
    14761 gtttacgccg catccggcaa cctgtcttta cgcggtagct tcatcctcgg ctaaacgcgt
    14821 ttcagcatcc gcgacgatag cttccagctc gctcaacatc tcttcatagc caaaattttt
    14881 ggcggtgcga gcaggttctt tcgccaccac gggcttcgct agctggcgct ttttggtcga
    14941 ttttttactc atcatcatct ccctgtaaaa acaactagac aggcaaatct atcagcaaag
    15001 cgcgcagtgg ggaatcggca accagcgtaa tgttagcctc gtcacgaata aacgccccat
    15061 caccgcaggt tagcgccgct ttctcttcat gatgcgtaag cgcatgaaat ttcccgtgaa
    15121 tcgattgcaa atacgcgcgt ggcccatgca actggaaatt cgcactttcg cctttgtcga
    15181 gcacgatatg gtgcagccac acttgttggc gtaattgcag gcttcccatc gcaccctctg
    15241 gcgaggcgat taattgctgc ttgtccatat taagcgccag cttttgaatc agcggattct
    15301 ctcgctgcgg gcaggtatcc agccaaagct gcattcgcgt taacggtttg tctttgctga
    15361 gattgtgttc gctatagctg acacccggct gggtagagag cagcaacgcc tctccggcgc
    15421 tggcctgaac atgattgcct tcgctgtcgc gatactctgc ttctccatcc agaatcacat
    15481 ttaaaatatc gactttggga taggttcgcg gctgaaaggc ggcacctggg gccagcactt
    15541 cctggttaag cacacgcagg gaggcatagc ctaacaattt cgggtcgaag tagtgtccaa
    15601 aggaaaaagt ataccgggcc tgcaaccatc cgtagtctgc ttgtccacac tgcctggcag
    15661 ttcgggtagt aatcataatt cttgacctct ctttacttcc ttttatggta aggggctgga
    15721 cgctacattg ttagccagat attctgcccg gtatgttcaa atttcctgaa tgagaacgaa
    15781 atggccaaag aaagggcatt aacgctggaa gcactacggg ttatggatgc gatcgatcgc
    15841 cggggcagtt ttgcggcggc agcggatgag ctgggacgcg tgccttccgc acttagctac
    15901 atcatgcaaa aactggaaga agagctggat gtggtgctgt ttgaccgctc gggccatcgc
    15961 accaaattca ccaatgtcgg gaggatgttg ctggagcgag ggcgcgtttt gctggaagcc
    16021 gcagataaac tgactactga tgcggaagct ctcgcgcgcg gttgggaaac gcatctcacc
    16081 attgtgaccg aagcgctggt accgacacct gcctttttcc cgttaatcga caaactggcg
    16141 gcaaaagcca atacccaact ggcaatcatc acagaagtgc tggcgggggc gtgggaacgg
    16201 ctggagcagg ggcgggcgga tatcgttatc gcgccggata tgcattttcg ttcctcgtcg
    16261 gagatcaact cgcgcaagct ctatacgtta atgaacgtct acgttgccgc gcctgatcac
    16321 ccgattcatc aggagccgga accgttatct gaagtgacgc gtgtgaaata tcgtggaatt
    16381 gcggtagcgg ataccgctcg tgagcgcccg gtgttgaccg tacagctgct ggacaaacag
    16441 ccgcgcttaa cggtgagcac gattgaagat aaacgtcagg cattactggc ggggcttggc
    16501 gtggcgacga tgccgtatcc gatggtcgaa aaagatattg cggaagggca gttgcgtgtc
    16561 gtcagcccgg aatcgaccag cgagatcgat attattatgg cctggcgtcg tgacagtatg
    16621 ggggaagcga aatcctggtg tctgcgggaa attccaaagt tgttcgctgg aaaataatct
    16681 attaagcacc tttcacaagg tgcttctgac tgctgacaaa cgcaaaactg cctgatgcgc
    16741 tacgcttatc aggcctacgc agtcactgca atatattgaa ttttggcaat ttgtaggctg
    16801 gataaggcgt ttacgccgca tccggcataa acaaagcgca ctttgtcaac aatttgaagc
    16861 accttttaca aggtgcttaa tcattattct tgttccagtt ttggatctgg tccaaagcgg
    16921 ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca gccgataaac
    16981 gggattaaga atagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag gcggcggaac
    17041 tgaaccgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag gatgccttcg
    17101 ccaccagcgc gttgccagcc taacatttta tccagcagcc ccagcacgaa cgtaaagatg
    17161 atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg gaaaccgaca
    17221 taatttttta gtactttcag ataccagtcc atttttgcct caattttgct caatcacttg
    17281 tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata ttcgttcaag
    17341 gaattaataa agtgccgtga aggcgcgcta agactgatgg caagcgcaaa atcgccagat
    17401 gcgctacgct tatctggcct acgcggtcat tgtaacatat tgaatataaa cgctttgtag
    17461 gtttgataag gcgttcacgc cgcatccgac atgaacaaca tgcacttagc taattatcag
    17521 aggcacctcg taaggtgcct ttttttaacc gaagcgaaca tctcgtccat gcggttcatc
    17581 gagatcctgc cacggaccaa ttgaaataat ccccgtaggg ttgatggtct tatggctgcg
    17641 gaagtaatga ttacggatgt gatcgaaatt gacagtttcg gcgatccccg gcatctggta
    17701 gatatcgcgc aggaagccat acagattcag gtaatcgctg atgcggtgct tatcacactt
    17761 gaagtgggtc acatacactg gatcaaaacg caccagcgta gtccacaggc gaatatcggc
    17821 ttcggttagc tggttgccgg tcaggtaacg atgctggcct aaaatctgct ccagtcgcgc
    17881 cagcgattca aacactttcg ccaccgcctc gtcgtaggct tgctggctgg tggcaaaacc
    17941 agctttatac acgccgttgt taacggtgtc ataaatccag ccgttaagtt cgtcaatttt
    18001 cgtttgcagg gctggtgggt agtaatcacc cgcttttgcg cccagcgcat caaacgcggt
    18061 attgaacatg cggatgattt ctgctgattc gttgctgacg atggtgtggt tctttttgtc
    18121 ccacagcacg ggaacagtaa ctcgtccgct gtagtgtgga tcggcgtgga gataaagctg
    18181 atacagaaat tcatgttgat agagcgtatc tccggttgct cccggaaaac tgtcatcaaa
    18241 ggtccagccg ttttccagca tcagcgggtt cactacggaa acggaaataa acggttccag
    18301 tcctttgagt ttgcgcatga tcagcgtgcg gtgcgcccac gggcaggcga gtgaaacata
    18361 gagatgataa cgatctttct ctgcgataaa accgcctgtg ccagtggggc caggtgcgcc
    18421 atcggcagtg agccagttac gaaatgcgga agctgaacgt tgaaatttac cgccggtaga
    18481 tttggtatcg taccaggtgt catgccagac gccgtcaatc agttgaccca tttttctctc
    18541 ctccaatagc aaaaacgagg agcttcctcc tcgtttttta tcgttaagta tagcgcgtgc
    18601 ttaccacttt ttattcagca ggcggtcgat gctatacgcg cccggaccgg taattgccag
    18661 cagcaggaat ccgccagaaa ttgtcaggtt tttcatgaac atcagcgagt tgacgccttc
    18721 agcaaagttg ctgtgaaata aaaatgccgt cagcagcgta aagcccgcag taaacagggt
    18781 tgtggtgcga gtcaggaaac tgaataggat tgccagacca ccaccgaact caagcagaat
    18841 caccagcggc agcataaaac ccgggacacc cattgcttcc atatattgtt gggtaccaga
    18901 gtaagcggtg atttttcccc agcctgcggt aataaacaga atcggcatta aaatgtgcgc
    18961 taccagtaca ccaacatctt ctaatttttt catcatactc tccactgcgc cactcaggct
    19021 gctgattgtt tttttgtgca agggcgcggt attagcgtcg ttgctgtcga tggagagaat
    19081 cataaacgtg gtgaatgatg attgttagca aggaaaactg tcaaaaatat tcaaaaaatt
    19141 tgagggataa ggccggaatt gctccggcca gagggaagtt aaccgcgaag ctgttgctgc
    19201 ttgagggtcg ttttaaccag acgccaggcg ctccatacgc caaaaccgcg tctggcccag
    19261 cggaccagca tattaggatg gcgaatcgtc cagatcgcca tcacgctact gccaaccagc
    19321 gcccaggagc gcagacttag cagcatattc cagcgacgat cgtaagcgcc tgttgcctcc
    19381 agccattcac gacgactggc ggaaagatcc agccgttgtt gctggatctg gctaagcagt
    19441 tgcgccttac gtcgttcacg ttcgacttta ctgctcacgg gactcctcct cgagcagttg
    19501 ccgatcgttt gccagctcat ggcgtgtatg gcgcagcaag gtggatttac gcgatttacg
    19561 taacgtccag ataccgccaa tcagtgccag tagcagcaac accacggtgg tggcaatcat
    19621 cgcattcagg cgatattgcg gatcaaccgc ccaaataatt agcaccatca ggctcataag
    19681 accaaatgca gcgaaaagca tcgtcaggcc cagcatcagt aaaagttgaa agagattagc
    19741 tttttcctct tccagctcca ccaccgccag ccgcagacgt gtttccacca tttcaaccat
    19801 gatggaaaca attcgctgcc cgatgcccag aacgctttta ccgggccctt gcgcgtgatg
    19861 agtgtccgcc ataattaacg acgcgacagc agaacgccga gcactacacc gattgcagcg
    19921 ccaattccca cgccggtcca cggattttca cgcacatact catcagcacg cgccgccgcg
    19981 acacgggttt gtttggcgat ggcatcaccg gtttcaccca ggcgataacg gctctgtttc
    20041 agtgcctgct ccgctttgct acgaatctta ctcaactctt ctttcgactt ctcgccagat
    20101 gagctaagca cctcttccag cgtatcggaa agggatttca actcagcacg cagatgttcc
    20161 gtagtgtgtt ctttcgacat agttttctcc aggtgagtaa gtagtgacta ttgtgagtta
    20221 gtagtcgcgc gcttccagct ttttcagctc ttcctgcgct tctgccagtt tccgttctat
    20281 cggcatcgcc tttttgcttc gcctcggcta aatcttgctg gcgttccgcc acctcatctt
    20341 tctgctttgc gattttcttg tgatgatcgg cacgcagctg gctatctgaa cagttggccc
    20401 ggacttcact cagggcttta ttcagaccgt caatacgatt ctggttttgg tgtttttcgg
    20461 catagctgat ctccttaagg atattttgct ccttttcctg acacagggta gtggcataac
    20521 taccggcact aagagcaaag agagaaacag ctaaagcgat gcggtatttc attcttgaac
    20581 cttccattgt gattcggacc tattacaaag gaacgattgt ccagacaacg aagcggtagt
    20641 gttaaccacc gcctcattaa tcatagacag ttacggtgaa atcaccaaag tgagtgatat
    20701 gtttcggata acaggaagtt atccgaagcg atgagagtta tcccgtaacc gggtcagcca
    20761 ctgcatagcc tgactgttat tgtcctgctg cgcaatgatg tagccatggg gcaatgttct
    20821 gtcgaggacc gcttttgcgg cggcgctctg gtcgctggag tcaaatgtaa ttaacagagt
    20881 gtcgtttttg ggggtgatac ttttgaaagg aatgccatga gcgtcaaggt gatgccagat
    20941 cgaaaaaccg tctggcatcg ttgtgccttg atgaacggca cgaatcgcca gcgtagactc
    21001 ttgctggcga accgcggacc aggccagcaa cagcgtgccg accagaagta aaacagtgcc
    21061 ggaccaggct agctggcgaa gcgacatgcg aggtatttgc atcccttacc cccgatttcc
    21121 atatttcttt ttccataaca cgaccagaga acctgccagg ccaaacacca gcagcaccac
    21181 cgggagcagc atcaggcatg acatcagctg gtcctcgtac tttaaaaata ccggcgtttt
    21241 gccgagcatg taacccagag ttgtcaggat caatacccac agcagaccgc tcatccagtt
    21301 gaagaactga aagcgcgcgt tattcagccc tgataacccg gcaatcgtcg gcagcagtgt
    21361 tctgacaaat gcaataaagc gaccaattaa cagcgccgat aaaccgtgtt tatgaaaaag
    21421 atggtgtgcg cgttgatgat aatgcgcggg taaatgagat agccagtttt gtacggtgcg
    21481 ggtattgccc agccatcgcc cctgaatata gctgacccag caaccgaggc tggcggcaac
    21541 ggtcagcagc agaatcgttt gcgggtaacc catcgcgcct ttcgcaatca acacgccgac
    21601 caataccagt aaactgtcgc ccggtaaaaa ggccgccgga agcaagccat tttcaaggaa
    21661 caaaattaca aacaagacaa aatacaacat gccaatcatc gatggattgg ccagggtttc
    21721 aaaatcctgc gcccacaggg cttgcagcaa ttgggtcaaa agttccattc attattcctg
    21781 gtgaatcggt taacgccacg ctggttttaa aagatgcact accgcaatgt tattgctgtt
    21841 ttggtatgtt ctgcgggttt gatacatcaa cacagcctga actattccct gttaaaacgt
    21901 gagataagaa agcattcaaa agcataaatt caaattgaat ctaattgtaa caaaggtcaa
    21961 cgttgttcgt tatcaaaatt acatgtccgt ggagtctgat tttgctgatt gcaggggagg
    22021 gtggcgagag gatttacaca ggctgacact ttatactttt cgcttaccga cgagacaaac
    22081 aggcgcattg cagcgcctgt cggacaggaa tttcatttgc tgccgctggt ggccgtatcg
    22141 agatgcacta ccgggttttc ggcgaacaga tagcggtcgg cattgaactc gaaatcgtcg
    22201 ctggtttcgt taaataacat gattttggtg ttttccagat gctgccacat tgccagctta
    22261 gcagcatgag gatctttgcg aattaacgcc ttgagaattt gatcgtggtc atcacaccag
    22321 ttatcaacgg tacgggaatc aatgtgttcg tgtagttttt tccagtacgg gttatggcta
    22381 cgctgggtcc acattttttc aacgatggcc gccagggcgg agttctgcgt cgccagggct
    22441 acctgaatgt ggaactgtaa atcccactcg gaatcacgga agcattgttc gccgcgcgcc
    22501 tgttcctgaa tggccatcag tttcatgatg tcttgtttcg ttacctgagt tgccgcgaac
    22561 tcggcaatat tactttcgat gagctggcga gcctgaagca actcaaacgg accgtaattg
    22621 gcgaattcca tattattgtc agccgcctgc tgatgacgcg gctggttgga aaccacatga
    22681 atacctgaac ctttacgcac ctccacatag ccttcaactt ccagcatgat gatggcttca
    22741 cgaactaccg ttcggctgac gttcttttca tcggcaataa agcgttcggc aggcagttta
    22801 tcacccacca gatagacgcc ctgttcaatg cgctctttca ggtcagcagc aagttgttga
    22861 tacaaacggc gtggttcagt gatttccata tgcgtctccc ggagtggaat gcggacgaag
    22921 ttatcatacc actttattca tgggtatgcc attggtcata tataaatttg atgattgaat
    22981 taatgtatta aattaatatt gtgaatattt ataaattcct aaaaaataag tatcgtcatt
    23041 gtgaaagcta ctaaatttca gtaagttaat tcccagcctt taaatttaat gttaactaaa
    23101 aatgcttttt tatattgact tacaataaat ctacatctct aatacaccaa cttttttgaa
    23161 acgatattgt aagatcaaaa ttatatggtg gcgaaaatta atctcttatt acggcttacg
    23221 ccgcttttat tcgtgttcgc tccttttttg gcacagtcaa atatgactgt ttatcctatg
    23281 cctgtttcca ttaatagtca gggtgaaggc aacgttcgcg taatatctaa aagtaatgaa
    23341 gttcagtaca tcaaggcgac agtattccgt atcgataatc catcgacgcc tcaggaaaat
    23401 gaagttgaga ttaaatccgg tgatgcaaat catctggttg ttatgccacc taaattcgct
    23461 ttaccagccg gtagtagcaa aaccgtacgt tttgttgcga tggaaccaga gcaaaaagag
    23521 aaaaattatc gcgttaaatt tgaagcggtt cccagtattg atgacgttgc cacagataaa
    23581 aaagatctct ctatgcagtt aacagttaac ttaatttggg gaatcgttgt tagtgttcca
    23641 cctcagcaac ctatagctaa gttagaagta aattctgccc aaaaattaat taatgcaggg
    23701 aatcaacgct taaagctttt aacgatcgct tattgtaaaa ataatagcaa agaaaactgt
    23761 aagatacaga ccgtaaataa aaacatcttc cctggtcagg aaagaaatct tgaaagcata
    23821 tctggctacg acaaaatcgt tgtcaaatat aataactgga tcaccaaaga taatggcgag
    23881 tttgaactgg cggtccatta attatcattg taataaacga aggaatagta atgaaaaagg
    23941 tatttgcaaa atctcttctg gtcgcagcga tgttttctgt tgcaggctcc gcattggcag
    24001 tacaaaagga tattactgta acggccaacg ttgatgccgc tctggatatg acacagaccg
    24061 ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct gccggggcag ggtctgcaat
    24121 cttaccagtt gatgaccaaa atctggtcta acgacgttac caaagatgta aaaatgcagt
    24181 tagtgtcccc agcgcaactg gttcaaagcc tggatgctag caagattgtt ccgctgacag
    24241 taacctgggg tggtgaagaa attaaagctg atgcggcaac cacttttacc gcaaccaaaa
    24301 tctttgcttc cgatgcgtta actaacggtt ctctggctaa aaacctgatg tttgctcaaa
    24361 ccaccaaagg tgttctggaa acaggcatct accgtggtgt agtaagcatt tatctgtctc
    24421 aggatattta atccggataa cagaattaac acgtaacaaa gggaggggta gccctccctt
    24481 attttaactg ttttagtatt tgttatggat ttcaggattg ccatggataa aaaattactg
    24541 gctcttttga tcctggcgag tctcagcccg gcagaggcga cattaaccaa aattcccgca
    24601 gggtttgagg ttattgctca gggacagcag gagtatatcg aggtttattt ttcagggaaa
    24661 agtctcggta aatattatgc aatggttaat cttgataccg taacatttct tgatccagca
    24721 agtctatata acaagctgga actggatgta gacgatcaga aaatcgcgca tatagtgaaa
    24781 gaaaaattat cgcagccgct agctcgccac ggtgaattgg cttgcggtta tgtacgtact
    24841 gactcagggt gtggttttct gaataccgat acgctggaaa taatctataa tgatgaagaa
    24901 agttcggcaa cgttgtttat taatccgcaa tggaattcag ctttcgatgc gaagtcatta
    24961 tatttaaatc cagacaaaaa tactgttaac gcttttatac atcagcaaga catcaatgtt
    25021 ctggcacagg atgattacca atcgttgtct attcagggaa acggtgcgct gggaataaca
    25081 gaaaatagct atattggtgc acactggaat ttcaacggtt atgatgcaga tgatgtcagt
    25141 gacagtaata ctgacgtcag cgatctctat tatcgttatg attttttacg tcgttattat
    25201 gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg cacaaggcgg gaactttacc
    25261 tttaactttc tgccactcgg tgcaatcgat gggatgcgta tcgggtcgac actgagctat
    25321 ttaaaccagg cgcaaagcca gcaaggaacc cctgtaatgg ttctgctttc gcgcaattct
    25381 cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt tttatctcaa tagtggttcg
    25441 caatttattg ataccagttc ctttccgcca ggcagctaca gcgtggcatt aaaagtctac
    25501 gaaaataacc aactcacccg caccgagctt gtgccgttta ccaaaaccgg cggtctgact
    25561 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt ttctgatgat
    25621 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
    25681 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
    25741 gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc
    25801 ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac gttgggcttt
    25861 tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag ctataacgcc
    25921 ttaagctgtt atgaaagtat ttccgcgaca gtttcactga attttgtcgg ctggaatatg
    25981 atgctgggtt atacctgcac acaaaataac actgatgata gtttgcgttg ggataaacag
    26041 cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc tgaaactgta
    26101 caacttagcg cttcccgcgc tattgtgatg cgtgactgga ttttgagtac gtcagtcggt
    26161 gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta cttatcgttt
    26221 tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac caatgtgtct
    26281 acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt atcgcatacc
    26341 ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc
    26401 gagacgattt gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa caggatgaag
    26461 agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaactttg cagcttggtc
    26521 aaagtgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt gaggttaacg
    26581 acagtagcca gggcaatcag caggggacaa caaacatcat taacggtgca gggaatcgtg
    26641 aattaatgtt gttgcctggc aagctgcgct atcgcgaagt gtctgccagc tttaactaca
    26701 actatatcgg tcgtttatta ctgccggcag cggtgaaaaa attcccgatt gtggggctga
    26761 acagcgccat gttactggta gctgaagatg gcggatttac acttgaaatt aacggcagcg
    26821 aaaaagagct ctatctgctt tccgggcagc aattccttaa gtgtccactg agtgttgtaa
    26881 agaaacgcgc cagcattcgt tacagcggag atgttacttg tagtgtggtg acttattcac
    26941 aattaccgga gtcgattcag gttcaggcac agttgaaaca gcctaaatta cgtggaaacg
    27001 ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcgg cgatattggg
    27061 cttgtttggc acgctcactg gcgttcaggc agctcctgac gtgaccagtg aaattacgta
    27121 tgatttggca tctggtagag cggattatta cttctggaag gatgaagcgt cggcaggaaa
    27181 taatggatat atgtggtatg aatgttcgta tcctgacctc caacaaacct gtacagctaa
    27241 tggaaatata tcgacagtac aaatctattt aactgaacaa cgcagtggga tgcgttggcc
    27301 ggtaaaactc aaaggattta aaacagccat tgtaagtagc gatgaagcgc cgctaggatg
    27361 caaggggggc aaagggcttc agacgaatct taaggattct aatagatctt catgtacaga
    27421 agatggtcaa cattattata tatacgatac aaagtttctt acgctctacc ttgagcagac
    27481 agagatgaag aatttgccga ttggtggcgt ctggaagggg aaagttaaat tacattcgaa
    27541 cagcccggcc caggactatt tcgcaaatat taccctgaat acgctcgacc ccaaccatat
    27601 tgacgtgttc ttcccggagt tcgcccacgc cacgccaagg gtgcagttag acttgcatcc
    27661 aacaggaagc gttaacggca gcaactacgc gcaagatctg accatgttgg acatgtgcct
    27721 gtacgatggt tttaacggta atgccatcag ttatgaaatc atgctcaaag atgaagggcg
    27781 accagctgca gggcgcagag acggttactt ctctatctat cgtcagggag ggaccaccac
    27841 cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa ccggtgggca
    27901 aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga aacgtgtgcg
    27961 tccggtggta ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac ccttgactct
    28021 ggcagtcgat aaattcagcg tgatggataa acaggccggg tactacatgg gcaaattgtc
    28081 agtaatcttt acgccttcct tgccaaccat caattaaccc tgcaaaccgg acaaacgctt
    28141 ttttctatat aaacgacgaa gccgcctgat aaggcggctt cgtcgttata acttacgtta
    28201 aacgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca atcgccggtt
    28261 tgttctgcaa tacggtccag ataaccagcg cacccagcag gtcaaatact gccagaactg
    28321 cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc gcaaacagcg
    28381 tacttgccag ccatgcggac atcccggtta aaccgttcgc cgtcgccact tcgttacgac
    28441 cgaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtgg gcaaaaccac
    28501 cgatacacag cagcataatt gcgacatacg ggttggtgaa cagaccgatc atacccgggc
    28561 caatcatcag cacagcaccc agcgttacga ccatcttacg ggaaacgatc aggttcacac
    28621 caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg aggtcagcaa
    28681 acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg taaactttaa
    28741 acatgaacag cgggatccac gcgttaaaag taccccaggc cggttctgct aggaaacgcg
    28801 gcagtgcgat accccagaac tgacggttac gcaggatctg accaacggac attttcttcg
    28861 ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt tcttcatcgg
    28921 tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc gcccagataa
    28981 agctcaatgc accggagatg ataaatgcca tctgccagct gtgcatcacg attgcccata
    29041 ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag tagcctactg
    29101 cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc gccgggatca
    29161 tcgctgcttc cgcggcaccg accgcaccac gagcaacagc caggccaccc cagctacctg
    29221 ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca taaccgattt
    29281 tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta taagcagcag
    29341 aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct tccatcagag
    29401 ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg ccgagcgtca
    29461 ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac ctcatcgttt
    29521 tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg ttagttacga
    29581 ccgggtaacc gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt tcgtgctaat
    29641 tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg caatttgcat
    29701 tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc ttaaaaggtg
    29761 agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag cgaggccagt
    29821 actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa ctacgtttat
    29881 tgatctaact cacgaaaata tcttcggact ctggaaattg gtgtgataac tttgtcagca
    29941 tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac tccgtttatg
    30001 actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga ctacgcaaaa
    30061 gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc ggaagactat
    30121 cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg gcgcgctatg
    30181 cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg tgaaaaattt
    30241 gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca ctggacgcac
    30301 ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc aactgccgat
    30361 gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctcctc gcgcggcatc
    30421 atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga ttctctggag
    30481 catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc gagctggcgt
    30541 ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc gaagctgggc
    30601 gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt gaccaaacgt
    30661 ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga cgtggtgatg
    30721 tttgctgaag cgaacgaagc ggaactcgac agcattctgg cgcgccgtct ggctggcgaa
    30781 accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt ccttggtgcc
    30841 gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg taataacaac
    30901 ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa cgaccgtccg
    30961 atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct gctgccgaaa
    31021 accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat gatcggtaac
    31081 ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg gtttaacgat
    31141 cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct gctgagccgt
    31201 tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca cgaatacttc
    31261 cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc accggcggac
    31321 atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg tgactacttc
    31381 gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc catgcgctgg
    31441 ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt gtcgataacc
    31501 agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta acggatatcg
    31561 caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg gcggatattg
    31621 cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat ctggatcagt
    31681 atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct gaagtgcaga
    31741 tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc ctgccaaccg
    31801 tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa gagaccaaca
    31861 acgccgaagg taccgacggc gtgttcctct tcagccacac ctacggctgc tcacaactgg
    31921 gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac ccgaacgcgg
    31981 gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca ttccgtgaaa
    32041 cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag caggatgatg
    32101 agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc aacgataaac
    32161 gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt tctgacggtc
    32221 tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg attgctaacg
    32281 gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag ttgctgatgg
    32341 accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac gacttcaaac
    32401 agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg aacaaagcgg
    32461 gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg ggttccagtg
    32521 tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg aacttgttaa
    32581 gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc tgccatatgg
    32641 tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg gtgaaaatcg
    32701 ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac gcgggtcagc
    32761 tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc atcgttgagt
    32821 ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg gcgatcttca
    32881 aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc gtttcataat
    32941 gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc gtattggctg
    33001 gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt caatcgtgac
    33061 gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt acattttttt
    33121 cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg tacattgatt
    33181 accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac tggcggaatt
    33241 ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac gattgtcagt
    33301 acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg gttttcaacg
    33361 tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat gattgagggt
    33421 agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca gaaaagggga
    33481 attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg ttaaacaatt
    33541 aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct gccgcagtga
    33601 acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat acgccgataa
    33661 taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac atattacagg
    33721 ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag gcacacagag
    33781 aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc agcgtattaa
    33841 ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg atggtggctc
    33901 ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca cacagcgcca
    33961 tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca ccgctttcgc
    34021 gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac accagcaatg
    34081 ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc agttgcgcgt
    34141 agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc ccaaaaatac
    34201 cgatcggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc gacatatcat
    34261 tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg ccgatcgccc
    34321 acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg gaaaccatgc
    34381 tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata tcacccgcgc
    34441 tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg gccagagctg
    34501 cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt ttctgcccgt
    34561 gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg gcaacagctt
    34621 tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg ggttttgaga
    34681 tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc aggctgccat
    34741 gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga tcctttcatt
    34801 gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt
    34861 ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc tggattttaa
    34921 agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca gagggaagga
    34981 ctggctatta aggctgatta gataacatat gggcaaaact atttggaata tgagaacggt
    35041 tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg cgcagggggc
    35101 ggccaatcgc cgccgccccc tgcaccctca ggctctggcg aacaaaatcg ccgctgcgcg
    35161 gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt ccctgtaaga
    35221 tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac caacacccgt
    35281 gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac ctttgtctgg
    35341 aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg ctgcttatca
    35401 tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc aaacaccacg
    35461 cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt gataccgata
    35521 aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc tacgcccgcc
    35581 agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt cagcacaatg
    35641 aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa aatcacgtcg
    35701 ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa tccattctta
    35761 cgcacaaaga aatgctcgtt gtcgatggtg tctgtcatgc gcaaatgacc gcgtagccag
    35821 cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc catcttcacg
    35881 ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg cgaaatcagc
    35941 cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac gccatacacc
    36001 agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat cagccagaca
    36061 aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa ggccagggcc
    36121 tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa agcggcgtta
    36181 aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt catggtcatg
    36241 gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat ggcgacaaca
    36301 acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt tccttacaca
    36361 taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata gacctcgcct
    36421 tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct ttcgggttgc
    36481 ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca atgaagttac
    36541 tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt ctgttacata
    36601 ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg aaaaatcacc
    36661 ccagtctggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac cgccagccct
    36721 ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc gtagagcatg
    36781 gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg cacaaagcag
    36841 actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc ctgaatctcg
    36901 cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa atcaccgtaa
    36961 tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc cgttgcctgc
    37021 acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa gccgatatcc
    37081 ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc gtccaggtaa
    37141 cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag tttgcctgct
    37201 ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt aaatgcctca
    37261 aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc cgccagattc
    37321 gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag ttgtgtctgg
    37381 gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc gctttccgcc
    37441 atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa gtgctgagcc
    37501 tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc atgggcggcc
    37561 tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat cataacgcgt
    37621 ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc ttcgacgtgc
    37681 gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc aatcatgacc
    37741 gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac cgcgctgaca
    37801 cgagcgagtt tctcccgcag ttgagtataa aaaccatgct tcatattgag gtctggcgcg
    37861 ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg ttggttatga
    37921 aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc acgcactacc
    37981 gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg tacgccttta
    38041 gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc gttatcgcca
    38101 ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt atcaatacgt
    38161 cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt acgcgccggg
    38221 acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac gcgatcggcg
    38281 ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag agtccggcga
    38341 gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt tatccttaac
    38401 gaaagggggg aggtcgattt taggcgctaa agaaagaaaa gataacgcct gacatcattt
    38461 tcagattttg tcctgcccaa gcgacaactg cgttagccac agccgggtgt cgaactcaag
    38521 ctggtggtac tgcggctcca tatggcagca caattgatag aacgctttgt tatgctcttt
    38581 ctccttcagg tgcgccagct cgtgcaccac gatcatgcgc aaaaacggtt ccggcgcgtt
    38641 gcgaaacacg gtagcgacgc ggatctctgc ttttgctttc agcttgccgc cctgcatacg
    38701 agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca tggatcttat tgtcatacat
    38761 cactttattg atcggcgggg cattacgcag aaactgattt ttcagatcct gagtatattg
    38821 ccagagagct ttatcggtgg cgtagtcgtg tgttcccgga tagcgttttg ccagcacatc
    38881 gcccagacgc tgttcgttaa tcagtgttcg cacctgcgaa agcagctgct cgggataacc
    38941 ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa gagaaaaagg gtatactcac
    39001 gcaccctttt caggggcaac gtcgaaattt tatcattcag gagggccgat gagccactta
    39061 gacaacggtt tccgttcact gacactacaa cgttttccgg cgacggatga cgttaacccg
    39121 ctacaggcgt gggaagcggc agatgaatat ttgctgcaac agttggacga cacagaaatc
    39181 cccggcccgg tgttgatcct gaatgatgcc tttggtgcgt taagctgcgc gctggcggaa
    39241 cataagccgt acagcattgg cgactcatac atcagtgaac tggcgacgcg cgagaattta
    39301 cgcctaaacg ggattgatga gtcgagcgtg aagtttctcg acagcaccgc cgactacccg
    39361 caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac tggcattgct ggaacagcaa
    39421 ctgcgtgcgc tgcgcaaagt ggtcacgtcg gatacacgta ttattgccgg tgccaaagcc
    39481 cgtgacattc acacttccac gctggaactg ttcgaaaaag tgctcggccc gaccaccacc
    39541 acgctggcat ggaaaaaagc gcgcctgatt aattgcacgt tcaatgaacc gccgctggcc
    39601 gatgcgccgc agaccgttag ctggaagctg gaaggtactg actggactat ccataaccat
    39661 gcgaatgtct tctcccgcac cgggcttgat atcggcgcgc gcttctttat gcaacatctg
    39721 ccagagaatc tcgaagggga gattgtcgat ctcggttgcg gtaatggcgt tattggtctg
    39781 acgctgcttg ataaaaaccc gcaggcgaaa gtggtgtttg tcgatgaatc gccgatggcg
    39841 gttgcttcca gccgtttgaa cgttgaaacc aacatgccag aggcgttgga tcgcagcgag
    39901 tttatgatca acaacgcgct ctccggcgtg gagcctttcc gctttaatgc tgtgctctgc
    39961 aaccctccgt ttcaccaaca acatgcgctg accgataacg tcgcctggga gatgttccac
    40021 cacgcccgcc gctgcctgaa aatcaacggc gagctgtata tcgttgccaa ccgccatctg
    40081 gattacttcc ataaactgaa gaaaattttc ggcaactgca ccactattgc gacgaataat
    40141 aaatttgtgg tgctgaaagc ggtgaagctg ggacgtcgtc ggtaagattg ggagttacct
    40201 gccggatgcg gcgtaaacgc cttatccggc ctacgttcag cacgttaccc ccaggccgga
    40261 taagacgcgg caagcgtcgc atccggcaat gtctgctcag ttctggaatc gcgcttccat
    40321 aaaacaaaaa atccctgtca ccatcctcta cattctcttg tttagcgttt ttctacgttt
    40381 attcttccgt cacacagata aattcatccg ttgccaatct gtcaacggat gttatcatat
    40441 tgccaatttg tcaacggacg tgatgaagaa tgcacctgat aactcaaaaa gcattgaaag
    40501 atgctgcgga aaaacacccg caacataaaa cggagttggt ggctctgggg aacacgattg
    40561 ctaagggata tttcaaaaaa cctgagtcat taaaagcagt attcccatct ctggataact
    40621 tcaaatatct ggataagcat tatgttttca atgttggggg caatgaatta cgtgttgtag
    40681 caatggtctt ttttgaatcg caaaagtgct acatacgtga agttatgacg cataaagaat
    40741 acgatttctt taccgctgtt catcgtacta aggggaaaaa atgattgcga ttgccgacat
    40801 cttgcaagca ggagaaaagc taactgctgt ggcacctttt ctggcgggta ttcagaacga
    40861 ggaacaatac acccaggcgc tggaactggt agatcatctg ctgctcaacg atcctgaaaa
    40921 ccccttgctg gatctggtgt gtgccaaaat aaccgcgtgg gaagaatcag cgcccgaatt
    40981 tgcggaattt aatgccatgg ctcaagccat gcctggcggt atcgccgtga ttcgtaccct
    41041 tatggatcaa tatggtttaa ccctttccga tctgccggaa attggcagta aatctatggt
    41101 gtcacgcgtt ttgagcggga agaggaaatt aacgctggaa cacgctaaaa aattggcaac
    41161 gcgattcggc atttctcccg ccttgtttat tgattaacgc gacgtgccgg atgcgttgca
    41221 tccggcgctt caaataacga tttaaatctc cagcgccagc cgtgttccct gggcaattgc
    41281 ccgtcgtgcg tccagctcca tagccacatc gcagccgcca attaaatgca cagttttccc
    41341 gctatcaatc agcggttgcg ccagcgcgcg gtttggctct tgccctgcgc agatcaccac
    41401 attgtccact gctaatacct gcgtttcgcc gttgatcacc acatgcagcc cgtcatcgtc
    41461 aatcttctga taacttacgc ctgggatcat tttcacaccc cgcgagagca gggtggtgcg
    41521 atggatccag ccggtggttt tgcctaaccc ctgtcccggt ttgctggctt tgcgctggag
    41581 catcacaatt tgccgtgggc tacgcgggat ctgcattccc tgcgggctta agccaccagc
    41641 ctgttgtagg ctgctgtcga tcccccactc attacagaac ctggcgatat tctggctggt
    41701 ggattcgccc ggctgactta aatacatcgc cgtatcaaaa ccaatcccgc cacaaccgat
    41761 gatggcaacc ttgttgccta ccggcgcttt gtcgcgcagt acatcgagat aactcaatac
    41821 cttcggatga tcgatcccgt cgataggcgg aacacgcggc acgatcccac tggcgaggat
    41881 cgtttcatcg aacgcctgta actgatccgc cgtcacggtg tgattgagtt tcagcgtcac
    41941 gcccgtcact tcgatcatcc ggcggtagta gcgcagcgtt tcgtaaaact cctctttgcc
    42001 ggggatctgt ttggcgatat taaactgccc gccgatctcg ctgtgcgcat caaacaatgt
    42061 tacctgatgc ccacgcgccg ccgcgttaat ggcaaacgcc agcccagcag gtccagcgcc
    42121 gaccaccgcc agattttttt tctgcacggc gggaaggatt ggcattttgg tttcgtggca
    42181 ggcgcgagga ttcaccaggc acgaggtgac tttgccaacg aagatctgat cgaggcaggc
    42241 ctggttgcag ccaatacaag tgttgatctc atcggctcgc cctgattgcg cttttgacag
    42301 cagttccgca tccgcgagaa acggtcgcgc catcgatact atatcggcat cgccgcgcga
    42361 gagaatatcg tcggcaacct gcggatcgtt aatccggttg gtggttacca gctgcagcga
    42421 gacgtggcct ttcagtttgc gcgtgaccca gctaaatgcg ccgcgcggca cgggcgtggc
    42481 gatggtcgga atccgcgctt cgtgccagcc tattccggtg ttgataatgg tcgcgcccgc
    42541 cgcttcaatg gcctgcgcca gttctaccgt ttcggcaaaa gtgccgccgc cttcgaccag
    42601 gtcgagcatc gacaaccggt aaataataat gaagtcgttg ccgacgcgtt cgcgcaccgt
    42661 acgcactact tctacggcaa atcgcatccg gttgcggtaa tcgccgcccc actggtcact
    42721 acgctgattg gtgcgcagcg tcagaaattc gttgatcaaa tacccttcag agcccatcac
    42781 ttcgacgcca tcgtatccgg cctcccgcgc cagttgcgcg cagcgggcga aatcgtcgat
    42841 cagttgcagg atctcttcat gggtgagttc gtgaggaacg aaacggttga tgggggcctg
    42901 caatgcggac ggtgcgacca gatgcggttg gtagctgtag cgcccggtat gcaaaatttg
    42961 cagggcgatt ttgccgcctt cctgatgtac ggcttcggta atggtgcgat ggtgtgggat
    43021 ctggctggcg tcgttgagca ttgcgccacc ttccatgcca acgcctatta aatctggtgg
    43081 aatgccgccg ctgacaatca gcgccacgcc gtgacgggcg cgttcggcat aaaacgcagc
    43141 cagccgttca gcaccgtccg ggtattcctc cagcccggtg tgcattgaac ccatcaacac
    43201 gcggtttttt aacgtggtaa aacctaaatc cagcggggcg aacagcgacg ggtagctcat
    43261 aaaattgtcc agtatgtaaa ataattgtta tgtggtcgga tgagttctaa tttagccgtc
    43321 gatggagaaa agggaaaagg ggagtgcggg gtttgtgatg ggattcaaaa agaatacgcc
    43381 cggtggtaac gccggatgcg acgcttgccg tgttttatcc ggcttggggg attgcacatg
    43441 tagggcggat aaggcgttca cgccgcatcc gccagccgtt gcaaattact gcttacggaa
    43501 aaaatcgtta tacaacatat acaaatgagc ggcactccag gagaaatttg gtgcgccttg
    43561 ctgcgcgcct gtcagcgggt tgtaattctc ctgaattggg ccatcggcgg ttaatccttt
    43621 ggcgtgcctg aagaacgtat ccgccagctt cagggcatca tcacgataac cgtaacgctc
    43681 catccctttc agaccaaacc agaactgatc cacccatacg cgcccgcgcc agtagatatc
    43741 agcgccaaaa gccggattgg ttaacgccgc cgttcccagc ggaacaaagg tgttgaactc
    43801 tttaggatcg agcatcacct tcaccaccgc gtcggcattg gcctgcgttg ccgcaccgtt
    43861 aaacagcggc gaccagcctt ccggcccttt accgcgctca acaatcggtt tgcccgcgca
    43921 gccgttcgcc agtggtttat cttcaatacg tacgtcatag tagaactggg tagtcgggtc
    43981 gaacatacag gtgttgatgt agtccgcgag ctgctgtgcc aactggcgat agcgtttggc
    44041 ctcttctggc ttaccgagga tggttgccat ctccgccaga taatggttat cgctgtacat
    44101 atagctggcc tgatccaccg actcctgcaa tagcgagtag cccagcaacg ttccgtcctg
    44161 actgcggttt tcggcgaatt tcaccgtcca gtcgctacgt ttgccgccat tagcgacata
    44221 tttatccaac tgttctttgt cgataaaacc aaagacggcg gcatcatcac gccccgattc
    44281 ccacgacgca gcgacctgtg ccggaatttc cagactgtcg tactggcctt tctccaccac
    44341 gcgggcgtag ttgttcagcc cagactgcgt ctcttcttta tcgccttttt tcaccgtaaa
    44401 cagcatctcg ccgctctcgg tgttgtgggc tttgtcgcgg gtcgcgccat attccggcac
    44461 cccgttgccg ttatgatcgc ggttacgtaa ccaccagtcg tgataggcca ccagtttcgg
    44521 gtacatctct gccaaccagg ctttatcctg ggtgacgttg tatacttcca tcaccgacca
    44581 ggcggcaagg ctgggtttgg tattacgttc gttccagttg ccgccatcgc caccacgctc
    44641 ggggctaagg ttccacgcta tcaggtcggg gacaaagccc acatcctgcg gacgcacgct
    44701 gtcgccaggc tggatctgcc aggagaagac cgcgcggata ttctctttgg cgatatccgg
    44761 attgaaatgc gccatcgcaa acgcctgctt ccaggtatcc cacggccagg tctgattgcc
    44821 ggagaaccag cgtccggtca ccgacggcgt gacggtgtta taattcaccg caccgcccgg
    44881 tgagcgccag ttaccgttga gcgtttcgat cgctttcacc gcgacgcgcg tctgttccgg
    44941 cgtcgcatcc ggattggtta accctttctt caggtattct tcccagcgtt gctgcgaggc
    45001 ggtgagataa aacgccggac gcgccagaat atcgcggatc tgcatttgct ctttgctaac
    45061 ttcctgagcg gtcagcagat gggaataggt ggtgtagagc gtggtcgaac cgttgatatg
    45121 cgccttactg gtaaagcgat taccgttgat ttcagtctgc accggcaggg atttatgcac
    45181 ctgatattcc gattcgccgg aggtcagcag atcccaggtg gcgcgcactt tgccaaaggt
    45241 gactttcagg ccatcacggg tggcgctgat tttgcgctga tagtcagggt attcgccagc
    45301 aatggtttta tcggaaagcg gtttcccttc tttcgcttcc agtttttcca gcagttcgcc
    45361 atcccacacc agatccagcg gtttattgct gatgatttta gtttccagta gtgacgtgcg
    45421 cggcgtggcg aagcgcagag tcatttcgac ctgcacatct tttgctgtca gtttttgcac
    45481 cagcgcgccg ggaatactgt atgcctccag cgtgaagttg actttcttgc cgtcttgcca
    45541 gacagtcagg cggtcgaaat tgctggccat aaagttgatg tattcttccg tcagcagcgc
    45601 aacgcccgga aagccgccca tggtgttcgg gccgtctggc aacagatgac catgccaggc
    45661 tccgagatcg aaaaacggat taaaacgctg gtgatcgtcg taatcgtaat ccttcatgta
    45721 ctgcggcgca ccagtacggt taatcacgtt tttataattg tcggcgttag cggcatgagc
    45781 ggaaaaactt atcagcagag cgcaggttac tggcgttaaa atagttttga ttttcatgaa
    45841 attacagtcc ttattaccag tagaaatatt gcatcaggaa gatttggtcg ctgggatcgt
    45901 tagaggcatt tttcatgtag aagcggctat caaaagccgt ggcgaaacgt ccgccgccat
    45961 attcgtacca gataccaaac tgcatgaaag aggtggtttc atcgtcagcg tggtcagggc
    46021 gatgtttggc gtaggtgtaa gcggtggaga gataaacgcc ctttaacgct tcatacatgg
    46081 cgctcaccat catcccgctt tcacttcccg gtacgtccag cccgtcggca cgtccggtat
    46141 ggtgccaggc gcgagcggca aagttagggg taaacaggcc cgtgagatag agttgtcctg
    46201 tgccgtcggt aatttccaga ccattcatcc aggtgatgcc ttcggcaagg tcatactgga
    46261 tgtaaccgtt catccattgc cggataggtg tatttgtcgt cgtagcgatc gtacttgcca
    46321 aaatgtagcc aggctttgct ttcgtcatga tgtccggcag gtgtggcggt gacgctataa
    46381 cgaaagttgc cgcttaagtt ctgtaccttc agcgcggtca taatgtcgcg ggtattggga
    46441 atgacgtagc ccaaatcggg tgtgaagtcg ccccaccatt gcaggtcatc cagcgaggaa
    46501 tcctgacgca gactgagcat gatttcggtg ccatcattgg tacgataacc gccatagaaa
    46561 cggttaatcc caccttcgaa accaccccag ctatggtcag gcacctaggc attaccgtcg
    46621 tggtcggcct gcacggtcca gccttcgccg taaatcaggc cgaaccagga gccgtacttc
    46681 atctccatgc caccttcaat ataggtgccg tcgctgtatt tatgtttatt ctcaccctct
    46741 aaatccatat atccaccaag ccctaattcg ccgtaaaaac gaaatgaagg agaagatgtc
    46801 gaggtaattg cgggtgtttt tgtgtcgtca tgttctgccg gacgtgcttc ttctcctgcc
    46861 gcagaaaagg cgtagcaaaa cggcagagca agtaatgctc tgcatggtgc agtgattaat
    46921 ttcattgtgt taatccatga gctaaagaaa aagtacagcc ttttatcgtt gtaattataa
    46981 aaatataaag gcagaaacta ttttccctct cctcatccag gaagagggaa aatgcctgct
    47041 cattttttat tagtctgctt aatcaacatt gctggctggc gtcgcttcaa tatggcgctc
    47101 ttcagccgtt aatcccttta tatatttact gtatttccac cacgcaaagc cgaggaagat
    47161 aacaataccg ccgacgttat aaaaaatgat ggtcagaata ttcgcgccag tcgggaatgt
    47221 cgaagcgaca aaccctacgg caaatatcgc aatcagcatt gaaacaacga taataccggt
    47281 gcggcgggag cccatacgga aatcgcgtgg caggtgatct aatttggcgc gtaaattcag
    47341 gtaagccagc atgatgaata acggcggaag catggacgct gcggcggtca tattaataat
    47401 ggtattcatc agatcctgca cggtattgga accgagcatc gggataatca tcagcgggat
    47461 gacgatcaga aactggatcc atgctgcgcg cgccggaacg ccgttttcgt taagttcgac
    47521 ggttttctta ccaaagatgc cttccgggat ttcggagaag aaaattttca ccggcgttgc
    47581 ggtccacatc agcagggaac cgaacattgc ggtaaaggac accagaccga caaagcgatt
    47641 catcaacgct tccggcagac caaaatacgc cgccatgccg tggaatacct gcaccgatcc
    47701 gccggtaaat ttcaactctt tgctgctgac gaagacgtta atcagcaccg aggagacgga
    47761 atacagtacg ccgataaaaa tcccggcgag gatgatcact ttaacgaacg atttcgaacc
    47821 gcctttgacg tcgttaacgt acaccgcgac ggactccgcg ccacctgcgg ccataaagat
    47881 ccaggtggta acgccgagga acgcccagtt gaagttcggg atcatcgcgt caacggtgat
    47941 cggatcggca ggctgtacgt cgccaaccag tgccgtacct gccagtaaaa tgtaggagag
    48001 cgttaacagc agcatcagcg ttgaagtgac ggaggtaatt ggccccagca ttttcgcccc
    48061 gttggtggaa acccaggtgg agaaggcgaa cagcaccata ctgataacgg tggtggcaac
    48121 cggcgtcata atatattcgt agccgaggaa ggcatacgaa gcataagcaa taacgcgcgg
    48181 caacagcgag gtaaaaaaga acagatttac gaaccagtaa gtgtaggcag taataaatgc
    48241 ccaacgtccg cccagcgaac tttttaccca cgcgtagaca ccggcttctg agtttttatt
    48301 taacgaaaca aattctgcga tgatcagaca gaagggaata aaataaaaaa tcgtcgcgag
    48361 gaaaaacatc ggtgccgagg ccagtccaag ctcaatatta ttattgataa cgttgttaaa
    48421 gctgtaaacg gcggcaaaag tcagcgagag caagccgaat ttgccgattg tattacgttt
    48481 ggtatcagac ataacacatc tccgaaaatg tgtagggtag cggtggcggc aaactccttt
    48541 tgccgccgta gttgttattt gttatggaaa taaccatctt cgatggtgac tttgagaacc
    48601 acttttttga ccgcgttatt gcagataaac cgatacgctt catggatatc gcaaataacg
    48661 atttgccctt cgtggacctc tacggtttct ccgcagcctt ttaaatattc acggtcagtt
    48721 tcatcacgat aatattccac tacctgtaat gtctctttcg gcgcatattc aattttttgc
    48781 tgcccttgca ggtagtaatg cacttcaaaa tagcgtcgat gcccggtaaa tagcgcatcg
    48841 gtcgcggagt cattttccac acggtaggtc aatgaatcac cgatggagtg ggcgacgcca
    48901 ggctgaatgt tgtcgatatt ttcaatcgct tcaacgcagc gttgccactt cttgccagag
    48961 gcgtaaatct ggcggaactg ttctaagtta tcgatgatcc tcattgctta ttctcgctgt
    49021 gcaaattcgt ggaaaagaac cctgcgccga actcatacga cgccaggctt tgcgcggtag
    49081 cttctccgcc agaaaccggc agcaacgtaa agccgtagct gaagtcacgg aaccagacgc
    49141 gccaggagtc cagcacctcg ctgccccagg agttggagcc gaggccaagt agctggtggt
    49201 cgaggttcag ggtgatgtca tcactgcgct gcagctcgtt acagtgctgg gcagcgtgga
    49261 tgttttcctg ggtatagtgc caggcgctga agttaattgg gcgctgcgga accaccagca
    49321 gaccgttgtc gtggcggtta gtcagtgccg tccagcggac atgctgacgg ttgccgttgt
    49381 tctgcgggaa gggatagttc tcgaacatgg catcgacggt gctgcgccag atatcgatga
    49441 tgttagcctg ctggctgtcg gcgtagtttt cgcccggtcc acgaccgtaa tacgccacct
    49501 gatcgtattc gccgttaatc cccatggtga aaccgatgca cggaatgatg tgcggatagt
    49561 cgccgtaacg ctcgccggaa agcgccacgt taacctggcc atcggcagcg atgcgccaga
    49621 tgtaggtaca gcgcatcccg aagtcaaaca ccggcggtgc aataactgtg cggttgatga
    49681 tcaacacttc accatcgctc tgttctacgg caaagtcgcg cagatgttcc tgcatgatct
    49741 gcaaatgatt cggctgccac aacccttcgt actcctgctt gtggttgtcg atcatcggct
    49801 tgaagaagtt gatctttggc tcgcgggtca gcagcgactc gccatttacc tgccaggatg
    49861 tcggtttgcc actcattttt gagaaggtga tcgcgaagtt gtagccgcga acggtgcagc
    49921 tcaaacgatc gtcttccagc gtcagcggac gcgcattatt tggtgcgaaa ggcactggct
    49981 gcgcggtgtt ttccttcagc gggaactgat aagtggcgat agaatgcccg gcttcgctgt
    50041 agcgggtgcg ggaatctttg gtcaccgtaa tgttgaggaa cgcttcgcgg gcgtccagct
    50101 gcggcagcgt gatctgcaag ggggcttcgc tgttcggcgc aacgtcgcgc agtttaatct
    50161 gctgcgtcgc gagcgtttca ccttcggcgc gcacctctgc gtgcagggtg tagtcatcaa
    50221 gcgtggtaaa ccacagttta ttttcgactt tcaattcgcc gcgagtcagc gcgtggattt
    50281 ttaccggcgc gataacctgt ttgtactctt tcaggcccga tcccggcgtc tgatcggaat
    50341 agatcaaacc atcaagacag aagttatagt tgttgggata gtcgccgtag tcgccgccga
    50401 atttatacca gacattgccg tggtcgtcct gtgcctggat cccgtggtcg caccactccc
    50461 agacataatg accctgaatg caatcgtgct tatagaagac gttctggtac tccgtcagcc
    50521 cgcccggtcc gttccccatc gcatgagcat attcacagat gatgcgcggc ttcggatgcg
    50581 ggtattcacc aaactcattc atcagcggca cgcgggtgta catggtggaa ataatatcga
    50641 ccacttcagc atcgcgatct tcttcgtaat gcaccagtcg cgtgtcatcc agcgctttcg
    50701 ccgcatggta catcgcgcgg atgttacagc catagccgga ttcattgccc agcgaccaga
    50761 tgatgatcga cggatggttt ttctgtgcgt ggatatggcg aacaatgcgc tcgacgtaga
    50821 ctttttccca ctgcggatcg tcggtaatac ggctgatatc gccgacatta gcaaagccgt
    50881 gcgattcgac gtcggtttcc gccatcacaa acaggccgta gatatcacac aattcgtaaa
    50941 aacgcggatc gttcgggtag taagcggtac gcacggagtt gatattgtgc tgcttcatca
    51001 actggagatc tttctcgacg cgatccattc caacggcgcg gcctttgcga tgatcgttgt
    51061 cgtgacggtt gacgccgtgc agcatcacat aacggttatt gatccagaac agaccgtcgc
    51121 gcactttgat atcacggaag ccaacgcgtt gtggcaccac ttccagaacg ttgccgttgg
    51181 cgtctttcag cgtcatgacc agatggtaaa gataagggga ttctgctgac cattgctgcg
    51241 gctgttcgac agtaaaagca aagctggcgc tggtcagttt ttcaattgcc aaatgatcaa
    51301 tggcgctgct gtgcaccacg cgttcgccat caaacagggt atattccagc gtcgtgacga
    51361 caggggaggc ggcgagattt tccagcacca cttcgcagga aagcgtggca tcgcaatagg
    51421 cttcgtcaaa gtcggtacgc acagtgaaat cgttaatatg cgttaggtgt tttccgacca
    51481 gataaacatc gcggaagatc cccgccgacc accacatatc ctggtcttcc acgtaggtag
    51541 agtccgccca ctgcatcacg cgcacacaca acaggttgtc gccggtttta accatcgcgc
    51601 tgatgtcaaa ctctgcggtc aggcgactgc ccttgctgaa acccacatac tgaccgttaa
    51661 cgtagacttc aaaataggtt tcgacgccgt caaatttaat cagcgtctgt ttaccctgcc
    51721 agccgtcgct gagggtgaaa atacgttgat aggcaccggt tgggttatcg ctggggacaa
    51781 acggcacatc gatggggaac ggaaaacctt cgtcggtata ttgcagtttg ccgtgacctt
    51841 ccatttgcca catggcgggg acggtaatat gcccccagtc agccattaac tcagaggtga
    51901 aggcttctgg tacttgcagc ggatggtcaa aaaagtggaa attccactga ccgcttaagg
    51961 gcagaaacag gctgctggtt tcgcgggcaa aggtacgcgc ttgcgcaaca gaatcatatg
    52021 aaaaaaagta cgcacgcggc gcaagtcggt tttcgtgggt gagctgaatg ttttcccagc
    52081 gattcatagg gcctcccggt agagagaaat cggaaccatt catggtgatg gtagtttgtg
    52141 taattgctgg gggtttagta aaatttttac taaaagtagc aggtaaatgc atttacttta
    52201 actggatcac gaaaataaac ggcagcactg tccggcaaaa atgattgccg gacagtgtgt
    52261 aaggggattt aacgggtcgt gccgcgcagt tttaatttgc tgggaacgaa gactagcagc
    52321 ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt taacgccctg acttcccatc
    52381 atctcggaat ggatgcgcac ggtggagagc ggcggaaagg taaatcgcgc ggtggggata
    52441 tcgttaacgc tgataagcga aatatcttgt gggatgttca ggccacgttc atgaattgcc
    52501 cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca gtgccttcgg atagtcttcc
    52561 cgcgccagca tttgttttgc cagttcataa cccgacgaac tggaaaaacc gccgcgccag
    52621 atatcctctt cgcgtaccac ttgtttcagt cggccatatt ccgcaaaggc gacctcacga
    52681 atatccgcct tgccaggctc atcttcaccg ccaataaaac caatacgatt aacgccctgg
    52741 ttgatataga agtcgatgat ttctttactg atgcgtgcca gatcgatatc caccgcatcg
    52801 taaccgctgc cgggttcgtg aaagtcgata aaacagatat tgtcggtcaa cgcgctggca
    52861 gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa taccggtgac gtttttaatg
    52921 tctggtaatc cgttgtgttc ataacagttg ttcagttcga tcgccagctt ttcgcactga
    52981 gtttcaatgc cgtggcggat cgccagatag taaggatcgt tgatctccag ctcctgctgg
    53041 tagctgtaga tagccagaat atggtgttgg ttgactgcgc ctgtctgtag tttacgggcg
    53101 ctactggtct tgtactccag cttttcggcg atctcgagaa tgcgatgttt cgtctcttct
    53161 ttcacattca atgtcggatc gtcatttaag accctggata ctgtcgccag ggatacgcca
    53221 gcttcgattg cgatgtcttt taatgttgcc atgtttacct tttttacttt ccctgatgtg
    53281 cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt ttcagtaaat aaagcgcgtt
    53341 tctttgacgc tgtaacggta acacagcggc ttaggaatgt gatcacgcct gcattctcca
    53401 tttttagcct acaggattga gaaaaacttt tgctatagtt tagtaaaatt ttacccgaag
    53461 ggaggaagca atggaaaccg tggcttacgc tgattttgca cgtctggaaa tgcgcgtcgg
    53521 aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag ctgtacatcg tacaggttga
    53581 tgtggggaaa aaacactgca aaccgtgacc agcttggtgc cgtactacag cgaagaggag
    53641 ctgatgggga aaacggtggt ggtcttatgc aatctgcaaa aagcgaaaat gcgtggtgaa
    53701 acgtcggaat gcatgctgtt gtgtgcggaa accgatgacg gcagtgaaag cgtgttgtta
    53761 acgccggagc ggatgatgcc tgcgggcgta cgcgtcgtat aaaaagatcg gatggcgacg
    53821 tcgtatcgcc atccgatttg atattacgct tcttcgacac ttactcgcat ggccgccagc
    53881 gccttacgcg ccgctttgat caccagttca cactgttcaa tggtcagtgt cagtggcggt
    53941 tcaatgcgga tcgttttggc gttattgagc gttccggcca ccagtacgcg ctggcggaac
    54001 atctcgctgg caaagttata gccgatttcg ttatcaacaa actcaatcgc catcaacatc
    54061 cctttaccac gcgcttcctg taccagatcg ggatattccc gcgccagttg acggaaaccg
    54121 tccagcaaca cattgatggt cgccagcgcc gccgcacagg ccagcgggtt gccgccaaag
    54181 gtggtggtat gcaggaatgg gttgtcgaac agaactgaaa acacctcttc agtggcgatg
    54241 gtcgcgccaa tcggcatcac gccgccgccg agggcttttg ccagacacag aatgtccggc
    54301 tgaacgttct catgttcgca ggcaaacatc ttgcccgtgc gccccatgcc cgtttgtact
    54361 tcatcgagga tcatcagtgc gccgaactca tcgcacagct tacgcacggc ggtgagatag
    54421 cccggcggcg gcagaattac gccaccttcg ccctgaatcg gttcgaggat caccgcagcc
    54481 acatcatcac cggttttttt gcactcatta agagccgtgc gcatggcttc gatattgcca
    54541 aacggcacat gacggaaccc tggcagcagt ggcataaacg gtttgcggaa ggtcgatttc
    54601 gccgtggccg acagcgcgcc aagtgattta ccgtggaacg cgccgctggt ggcaataaaa
    54661 gtaaatttgc cgcgcggcga ctgataagcc tttgccagct tcagcgccgc ttcaacggac
    54721 tcggtgccgc tattacagaa gaagctgtat ttcagtttac cgggcgttag cgcagcaagg
    54781 gttttcgcca gcatagcccg tagcggatcg agtagctcct ggctgtgcag tggttgtttc
    54841 gcaagttgat tctgtacggc ggaaaccaca actggattac ggtgccccac gttgaaaatt
    54901 ccaaaacctc ccaggcagtc gataaactcc tgtccctggg tgtcgacaag cgtatttaaa
    54961 cctcccgctt gccactctac ggctccgtaa tccccgccgg cggtaacaga tttgcgatac
    55021 tctaaaaacc ccggattgac atgctctttg aagtattcaa tcacctctcg gttaagtgct
    55081 ttcatctcct catgatccag cgttcgcttc tcaatgagat tcagggcgtg ggcgctgcac
    55141 gctaaagccg atgcgctcga aggtaacctg ttcaaaatat gctccggagg ctcgcgtatc
    55201 acatgatacc gatttaagta ttgcagggat tgcgccactc cggctgcgat cgcgaaaatc
    55261 gggataaaca caaatgcggt tatcatttgt gcaatattta atgctatccg ttaacatttg
    55321 ttgttatttt gcacggtttt gtcgggtatg gatttgatga ttgctgcgca aatgcacgtt
    55381 ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt atagtaacag
    55441 agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat tgcgatctaa
    55501 atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg acaagaagtt
    55561 aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg tcacccagca
    55621 aaataccccg ctggcggacg ataccactct gatgttcact accgatctgc aaagctatat
    55681 cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag agttacaagg
    55741 gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg cggatatgtg
    55801 gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc gcaaaaatgg
    55861 cgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa aaatcagtgg
    55921 ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg agccgctgta
    55981 caaagcgttg aactccggac gtaccagtaa gcgtattcat aaaggcctgg tggtgcgtaa
    56041 aggctggctg ggtaaactgc cttcattacc gcttcgctgg cggacgcgtg gagtgatgac
    56101 cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg tggtgacgta
    56161 tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc agattgtgcg
    56221 cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc gtaatagtgt
    56281 tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag ggcagcttgg
    56341 cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg tcagaaatgg
    56401 cagtgagacg ctggcgaaag gcaccgatga actgaacgaa catacccagc agacagttga
    56461 taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga aacagaactc
    56521 tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg cagtgcaggg
    56581 tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca gtacccagcg
    56641 cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata ttctggccct
    56701 gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg cagtggtggc
    56761 aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata ttcgcaagct
    56821 gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg ccgccggacg
    56881 gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg cccagattag
    56941 ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag tggatgagct
    57001 taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg tgtcggcgat
    57061 ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt aatcggtatg
    57121 ccggatccgg cggttggagc acaatacctg atgcgatgct ggcgcatctt atcaggcctg
    57181 cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc cggcaattgc
    57241 accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa gcggcgcggt
    57301 cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg ttgacgcgta
    57361 aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc tggcgcagcg
    57421 ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt tcgcggtttt
    57481 cttccagcca ctgcgcgcct tgttcggtca gcgcaatctg cttcttacca ccttcctctt
    57541 cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgtcgggtag atgacgcccg
    57601 ggcttggggt gtaattcccc tgggttagat tctcaatcgc tttaatcaat tcgtaaccgt
    57661 ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg tgaccaaaga
    57721 aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcaacattga ccgtgttcat
    57781 gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag cagccctcat
    57841 ggcgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata tcagcctcct
    57901 tttgcttgat tagatatatc taatttatat ctgatgtgag cattactgca agtacaaaaa
    57961 tgatttttaa acgtggaagc acctcacatc taggccggat aagacgttca ctccgcatcc
    58021 ggcagcattg ctatggcact gaatgccttc acatacttca gacatatcag ttctacaaat
    58081 cgcttgcatt tatcatgatt aacaatcatt atcatttgcg aattttattt agatatatct
    58141 gattaccatc acgaaggcga taacaatgaa taacaccccc cgctacccgc agcgcgttcg
    58201 caatgatctg cgcttccgtg aactgactgt gctacgcgtt gagcgcatca gcgccggttt
    58261 tcagcgcatt gtccttggcg gcgaggcgtt ggacggtttt acatcgcgcg gctttgacga
    58321 tcacagcaaa ctcttctttc ctcaacctga cgctcacttt gtgccgccaa cggtaacgga
    58381 agagggcatc gtctggccgg aaggaccacg cccaccgtcg cgtgactata cgccactgta
    58441 tgacgaacta cgccatgaac tggcgatcga tttctttatt cacgacggtg gggtcgccag
    58501 cggctgggcg ataaacttac ggtggcaggt ccgcgcggtt cgctggtggt gccggaagat
    58561 tacgcgtatc agctgtatgt ctgcgatgaa tccggaatgc ccgcactgcg ccgccgcctg
    58621 gaaacgttga gcaaacttgc cgttaaaccg caagttagtg cgctggttag cgtgcgggat
    58681 aacgcctgtc aggattatct cgcgcacctt gatggtttta atatcgaatg gctggcacat
    58741 gatgagcagg cggtagatgc gcgtctggcg cagatgcaaa tccctgccag atgattactt
    58801 catctggata accggcgaag gtaaagtcgt taagaattta agccgccgct ttgaagcgga
    58861 acagtatgac ccacagcggg tacgtgcagc ggcttactgg cacgcaaagt aactgatata
    58921 taaaaagaaa aaggctgacg atttctcgtc agcctttgct atatctggtg gcccctgctg
    58981 gacttgaacc agcgaccaag cgattatgag tcgcctgctc taaccactga gctaaggggc
    59041 catggtagcg gattataaag taactccgtg tcgcaatcca gccattaccg cgcgcctgct
    59101 gtttttataa acaatgtatt ttcaatccgt tatacttttc ctggtgatgt tagaaaggag
    59161 taaatatggt tgaggatatt ttggctccag ggttacgggt cgtgttttgc ggtatcaacc
    59221 ctgggctttc atccgccggg actggttttc cctttgctca tccggcaaat cgcttctgga
    59281 aggtgatata tcaggccggg tttaccgacc gtcagttgaa gccgcaggag gcacagcatc
    59341 tgctggatta tcgttgtggc gtcaccaaac tggtagaccg tccaacggtg caagccaatg
    59401 aagtttcaaa gcaggagctg cacgcaggtg ggcgtaagct gattgaaaaa attgaagatt
    59461 atcagccgca ggcgttggcg attctgggca aacaagcata tgaacaggga ttcagccagc
    59521 gcggtgcaca gtgggggaaa caaacgctca ccattggttc gacgcagatt tgggtgctgc
    59581 caaatcccag cggtttaagt cgcgtttcac tggagaaact ggttgaagcg tatcgcgagc
    59641 tggaccaggc gctggtagtg cgtgggcgat aaaaaacgcc accga
//
LOCUS       DADWTH010000013        59472 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.013, whole genome shotgun sequence.
ACCESSION   DADWTH010000013 DADWTH010000000
VERSION     DADWTH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.013"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNY02_000802"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNY02_000802"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBB9432446.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNY02_000803"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNY02_000803"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBB9432447.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNY02_000804"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNY02_000804"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBB9432448.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNY02_000805"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNY02_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBB9432449.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNY02_000806"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNY02_000806"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBB9432450.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILCEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNY02_000807"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNY02_000807"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBB9432451.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JNY02_000808"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JNY02_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBB9432452.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWCGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNY02_000809"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNY02_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBB9432453.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEMAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNY02_000810"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNY02_000810"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBB9432454.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNY02_000811"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNY02_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBB9432455.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="JNY02_000812"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="JNY02_000812"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBB9432456.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JNY02_000813"
     CDS             complement(15665..16702)
                     /locus_tag="JNY02_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB9432457.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="JNY02_000814"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="JNY02_000814"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB9432458.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17581..19303)
                     /gene="ggt"
                     /locus_tag="JNY02_000815"
                     /pseudo
     CDS             complement(17581..19303)
                     /gene="ggt"
                     /locus_tag="JNY02_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19423..19863
                     /locus_tag="JNY02_000816"
     CDS             19423..19863
                     /locus_tag="JNY02_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBB9432459.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNY02_000817"
     CDS             complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNY02_000817"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBB9432460.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20590..21580)
                     /locus_tag="JNY02_000818"
                     /pseudo
     CDS             complement(20590..21580)
                     /locus_tag="JNY02_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNY02_000819"
     CDS             complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNY02_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBB9432461.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNY02_000820"
     CDS             complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNY02_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBB9432462.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNY02_000821"
     CDS             complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNY02_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBB9432463.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNY02_000822"
     CDS             complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNY02_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBB9432464.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNY02_000823"
     CDS             complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNY02_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBB9432465.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26601..27878)
                     /gene="livM"
                     /locus_tag="JNY02_000824"
     CDS             complement(26601..27878)
                     /gene="livM"
                     /locus_tag="JNY02_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBB9432466.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNY02_000825"
     CDS             complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNY02_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBB9432467.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28849..29958)
                     /gene="livK"
                     /locus_tag="JNY02_000826"
     CDS             complement(28849..29958)
                     /gene="livK"
                     /locus_tag="JNY02_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBB9432468.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLACSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30382..30765
                     /gene="panM"
                     /locus_tag="JNY02_000827"
     CDS             30382..30765
                     /gene="panM"
                     /locus_tag="JNY02_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBB9432469.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30964..32067)
                     /gene="livJ"
                     /locus_tag="JNY02_000828"
     CDS             complement(30964..32067)
                     /gene="livJ"
                     /locus_tag="JNY02_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBB9432470.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNY02_000829"
     CDS             complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNY02_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBB9432471.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNY02_000830"
     CDS             complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNY02_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBB9432472.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNY02_000831"
     CDS             complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNY02_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBB9432473.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35159..36604)
                     /gene="ftsY"
                     /locus_tag="JNY02_000832"
     CDS             complement(35159..36604)
                     /gene="ftsY"
                     /locus_tag="JNY02_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBB9432474.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAHTE
                     AEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVN
                     AEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQALAAEAAEEAVMVVPPAEE
                     EQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQ
                     LLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEDKT
                     PFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRAAAVEQLQVWGQRNNIP
                     VIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKIVRVMKKLD
                     VEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGI
                     PIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36754..37350
                     /gene="rsmD"
                     /locus_tag="JNY02_000833"
     CDS             36754..37350
                     /gene="rsmD"
                     /locus_tag="JNY02_000833"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBB9432475.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37340..37609
                     /locus_tag="JNY02_000834"
     CDS             37340..37609
                     /locus_tag="JNY02_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBB9432476.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37612..37971)
                     /locus_tag="JNY02_000835"
     CDS             complement(37612..37971)
                     /locus_tag="JNY02_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBB9432477.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38112..38738
                     /locus_tag="JNY02_000836"
     CDS             38112..38738
                     /locus_tag="JNY02_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBB9432478.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38812..41010
                     /gene="zntA"
                     /locus_tag="JNY02_000837"
     CDS             38812..41010
                     /gene="zntA"
                     /locus_tag="JNY02_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBB9432479.1"
                     /translation="MSTPDNHGKKAPQFAAFKPITTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41112..41357)
                     /gene="tusA"
                     /locus_tag="JNY02_000838"
     CDS             complement(41112..41357)
                     /gene="tusA"
                     /locus_tag="JNY02_000838"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBB9432480.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41578..42243
                     /locus_tag="JNY02_000839"
     CDS             41578..42243
                     /locus_tag="JNY02_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBB9432481.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42316..42873
                     /gene="dcrB"
                     /locus_tag="JNY02_000840"
     CDS             42316..42873
                     /gene="dcrB"
                     /locus_tag="JNY02_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBB9432482.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42877..44094)
                     /locus_tag="JNY02_000841"
                     /pseudo
     CDS             complement(42877..44094)
                     /locus_tag="JNY02_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44226..45275
                     /locus_tag="JNY02_000842"
     CDS             44226..45275
                     /locus_tag="JNY02_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB9432483.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLYLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTRVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45330..45917
                     /gene="acpT"
                     /locus_tag="JNY02_000843"
     CDS             45330..45917
                     /gene="acpT"
                     /locus_tag="JNY02_000843"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBB9432484.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46028..47602
                     /gene="nikA"
                     /locus_tag="JNY02_000844"
     CDS             46028..47602
                     /gene="nikA"
                     /locus_tag="JNY02_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBB9432485.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47602..48546
                     /gene="nikB"
                     /locus_tag="JNY02_000845"
     CDS             47602..48546
                     /gene="nikB"
                     /locus_tag="JNY02_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBB9432486.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPTTL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48543..49376
                     /gene="nikC"
                     /locus_tag="JNY02_000846"
     CDS             48543..49376
                     /gene="nikC"
                     /locus_tag="JNY02_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBB9432487.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49376..50140
                     /gene="nikD"
                     /locus_tag="JNY02_000847"
     CDS             49376..50140
                     /gene="nikD"
                     /locus_tag="JNY02_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBB9432488.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50137..50943
                     /gene="nikE"
                     /locus_tag="JNY02_000848"
     CDS             50137..50943
                     /gene="nikE"
                     /locus_tag="JNY02_000848"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBB9432489.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50949..51350
                     /gene="nikR"
                     /locus_tag="JNY02_000849"
     CDS             50949..51350
                     /gene="nikR"
                     /locus_tag="JNY02_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBB9432490.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51470..51829)
                     /locus_tag="JNY02_000850"
     CDS             complement(51470..51829)
                     /locus_tag="JNY02_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBB9432491.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51826..52101)
                     /locus_tag="JNY02_000851"
     CDS             complement(51826..52101)
                     /locus_tag="JNY02_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBB9432492.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52160..53284)
                     /locus_tag="JNY02_000852"
     CDS             complement(52160..53284)
                     /locus_tag="JNY02_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9432493.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53284..56019)
                     /gene="rbbA"
                     /locus_tag="JNY02_000853"
     CDS             complement(53284..56019)
                     /gene="rbbA"
                     /locus_tag="JNY02_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBB9432494.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQSFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQLTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56016..57083)
                     /locus_tag="JNY02_000854"
     CDS             complement(56016..57083)
                     /locus_tag="JNY02_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBB9432495.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57449..59071)
                     /locus_tag="JNY02_000855"
     CDS             complement(57449..59071)
                     /locus_tag="JNY02_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBB9432496.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKSQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINLGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        14061 a        16407 c        15060 g        13944 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgtttgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca caaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggtgcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 tttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgaa cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatacgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 gcccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgccgctc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca
    19261 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19321 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19381 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19441 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19501 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19561 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19621 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19681 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19741 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc
    19801 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19861 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19921 gcaactctgc cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19981 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    20041 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    20101 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    20161 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20221 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20281 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20341 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20401 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20461 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20521 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20581 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20641 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20701 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20761 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20821 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20881 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20941 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    21001 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    21061 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    21121 ccggatcacg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21181 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21241 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21301 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21361 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21421 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21481 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21541 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21601 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21661 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21721 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21781 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21841 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21901 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21961 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    22021 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    22081 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    22141 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22201 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22261 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22321 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22381 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22441 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca
    22501 ccgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22561 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22621 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22681 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22741 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22801 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22861 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22921 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22981 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    23041 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    23101 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    23161 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23221 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23281 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23341 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23401 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23461 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23521 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23581 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23641 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23701 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23761 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23821 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23881 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23941 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    24001 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    24061 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    24121 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24181 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24241 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg
    24301 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24361 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24421 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24481 ggagcgttgc cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct
    24541 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24601 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24661 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24721 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24781 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24841 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24901 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24961 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    25021 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    25081 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    25141 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25201 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25261 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25321 ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc
    25381 agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga
    25441 acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac
    25501 tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag
    25561 aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc
    25621 cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg
    25681 cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc
    25741 tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg
    25801 ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg
    25861 gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg
    25921 attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa
    25981 caagatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt
    26041 ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac
    26101 catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac tcgcctgacg
    26161 gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc
    26221 gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag
    26281 ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac
    26341 atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag
    26401 atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt
    26461 aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta
    26521 cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt
    26581 aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc
    26641 agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc
    26701 aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc
    26761 accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact
    26821 atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga
    26881 cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcggtc
    26941 agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac
    27001 gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac
    27061 accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc
    27121 aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg
    27181 agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca
    27241 gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga
    27301 tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc
    27361 agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca
    27421 aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga
    27481 ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata
    27541 tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg
    27601 aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg
    27661 gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc
    27721 gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg
    27781 gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa gaacatcgcg
    27841 gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac
    27901 ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat
    27961 ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc aggatcagcc
    28021 cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt
    28081 tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta
    28141 cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg
    28201 tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc
    28261 ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc
    28321 agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc
    28381 actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt
    28441 agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt
    28501 tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga
    28561 ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca
    28621 gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga
    28681 agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg
    28741 tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca
    28801 taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc
    28861 cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc
    28921 tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt
    28981 caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac
    29041 tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc
    29101 atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat
    29161 gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttccggccc
    29221 cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg
    29281 gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc
    29341 ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc
    29401 tttcagcccg tcctgcaccg aacacgccag cccttcgcca tactgttgtt tgtcgtgaat
    29461 gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg
    29521 ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg
    29581 gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg
    29641 ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt
    29701 gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag
    29761 cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt
    29821 aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt
    29881 aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt
    29941 tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt
    30001 atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta
    30061 aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta
    30121 agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca
    30181 taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat
    30241 ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc
    30301 aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt
    30361 tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac
    30421 caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt
    30481 gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg
    30541 gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc
    30601 cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca
    30661 tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag
    30721 gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt
    30781 caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg
    30841 tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc
    30901 cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa
    30961 caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa
    31021 gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt
    31081 cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa
    31141 agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc
    31201 tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt
    31261 caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc
    31321 ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc
    31381 catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc
    31441 caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc
    31501 attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt
    31561 gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt
    31621 cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt
    31681 cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc
    31741 agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa tgccgtcgtt
    31801 aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac
    31861 gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc
    31921 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac
    31981 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
    32041 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
    32101 tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc cgtgctttag
    32161 cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt
    32221 ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag
    32281 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
    32341 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
    32401 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
    32461 ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag
    32521 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
    32581 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
    32641 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct
    32701 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
    32761 ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
    32821 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc
    32881 gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc
    32941 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
    33001 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
    33061 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
    33121 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
    33181 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
    33241 ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
    33301 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
    33361 aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga
    33421 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
    33481 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
    33541 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg
    33601 acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca
    33661 gcagcgcacc accgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt
    33721 tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga
    33781 cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc
    33841 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg tcaatgccgt
    33901 taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt
    33961 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
    34021 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
    34081 tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca
    34141 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat
    34201 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
    34261 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
    34321 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
    34381 tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc
    34441 gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
    34501 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc
    34561 aggttaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
    34621 agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc
    34681 agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg
    34741 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
    34801 gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg
    34861 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
    34921 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
    34981 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
    35041 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
    35101 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
    35161 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
    35221 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
    35281 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
    35341 cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag
    35401 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
    35461 tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
    35521 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
    35581 caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
    35641 caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
    35701 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
    35761 tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa
    35821 tctcacccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
    35881 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
    35941 cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga
    36001 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
    36061 gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct tcaaccggct
    36121 gttcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg
    36181 cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt
    36241 cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc tcttcggcgt
    36301 tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt
    36361 ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa
    36421 caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat
    36481 gcgcctgaac gatttcttct acaaccggtt gttcattctg aacttctgtc tctttttccg
    36541 gggtctgctc tttttgacca aagcccagcc aggaaaaaaa gccacgtctt ttttctttcg
    36601 ccatttgcga ctacactcct cgctgttgct tcatggcaca gcgttaacgc tatgtacata
    36661 gcagctaaaa aaatgatgaa atagtctatc acttaactta attcacatca ccgcctgcaa
    36721 gtatgtgtta tctggcggat tgagcaattt atcatgaaaa aaccgaatca ttccggcagc
    36781 ggccaaatcc gcattattgg cgggcagtgg cgaggccgta aactcccggt tcctgatagc
    36841 ccaggtctgc gccccaccac cgaccgcgta cgcgaaacgt tgtttaactg gctggctccg
    36901 gtcattgttg acgcccaatg tctggattgc ttcgccggga gcggcgcgct ggggctggaa
    36961 gcgttatcgc gctacgctgc gggggcaacg ttgattgaga tggatcgcgt ggtttctcag
    37021 cagttaatta agaatctggc gacactaaaa gcaggcaatg cacgcgtggt gaacagcaac
    37081 gcaatgtcat tcctggcgca aaaaggcaca ccgcataata tcgtgtttgt cgatccaccg
    37141 ttccgccgtg gcttgttaga agagacgata aatttactgg aagataacgg ctggctggct
    37201 gacgaagccc tgatttatgt cgaaagcgaa gtcgaaaacg gtctgcccac tgttccggca
    37261 aactggtcat tacatcggga aaaagtggcg ggtcaggtgg cttatcggct gtatcaacac
    37321 gaagcacaag gagaaagtga tgctgattaa tattggtcgt ttgttaatgc tctgcgtttg
    37381 gggattttta atcctcaatc tggtgcatcc cttcccgcgc ccgctaaata ttttcgttaa
    37441 cgtggcgctg atttttaccg tgctgatgca tggtatgcag ctggcgctat tgaaatccac
    37501 tttaccgaaa gatggcccgc agatgaccac cgccgaaaag gtgcggattt tccttttcgg
    37561 cgtgtttgaa ctgctggtct ggcagaagaa atttaaagtt aaaaaataac cttattgttc
    37621 gccaacaaag ctaacaaagc gcgttccttt atagctcagc gtacctttat cgcccacggt
    37681 cagggcgtgg tactgctggg cgtcgagacg aaacgtctgc tccattcctc cgctttgcgg
    37741 tttgaagctt gcctcatagc gcatactggt gcctgccgga gtcacttcct gctggcgcga
    37801 acggcgatcg ttaatcggtt tttcccgctt gttgctcacc accaccagct tttgctggag
    37861 cggagccata tcattatcag ctttttcccg tcgctgttgc ataaaacgaa acgatgcggc
    37921 gacgacaatt aagccaatga taacaataaa gaaaagaggt ggtttactca tctttatccc
    37981 tcatcggaaa atgcggaatt aagcataccc tgccagttat ggtgttgtca tccgtccacc
    38041 ctcgctacta aactggaagc aagaccgtag gcattccgct tacgaaaaaa taacgaattc
    38101 aaggaactaa gatgctttgg tcgtttatcg ctgtctgtct ttccgcatgg ctatctgtgg
    38161 atgcatcgta tcgtgggcca acctggcaac gctgggtgtt taaaccgtta acccttcttc
    38221 tcctgctgtt actggcctgg caagcaccga tgttcgacgc cattagctat ctggtgctgg
    38281 cgggactgtg cgcctcactg ctgggcgatg cgctaaccct gttgccacgt caacgtctga
    38341 tgtacgccat cggcgcgttt ttcctctcgc acctgctgta caccatctat ttcgccagtc
    38401 agatgacgct ctctttcttc tggcctctgc cactggtgtt gctggttctg ggtgcgctgc
    38461 tactggcgat tatctggacg cggctggaag agtaccgttg gcctatctgc acgtttatcg
    38521 gcatgacgct ggtgatggtg tggctggcag gtgaactgtg gttcttccgt ccgaccgctc
    38581 cggcgctctc tgcgtttgtt ggcgcttcgt tgctgtttat cagtaacttt gtctggctgg
    38641 ggagccacta tcgccgacgc ttccgtgcgg ataacgcgat tgctgcggcc tgctactttg
    38701 ccggtcactt cctcatcgtc cgctcactgt atctctgata aaacttgact ctggagtcga
    38761 ctccagagtg tatccttcgg ttaatgagaa aaaacttaac cggaggatgc catgtcgaca
    38821 cctgacaatc acggcaagaa agcccctcaa tttgctgcgt tcaaaccgat aaccacggta
    38881 cagaacgcca acgactgttg ctgcgacggc gcatgctcca gctcgccaac gctctctgaa
    38941 aacgtctccg gcacccgcta tagctggaaa gtcagcggca tggactgcgc cgcctgtgcg
    39001 cgcaaggtag aaaatgccgt gcgccagctt gcaggcgtga atcaggtgca ggtgttgttc
    39061 gccaccgaaa aactggtggt tgatgccgac aatgacatcc gtgcccaggt tgaatctgcg
    39121 gtgcaaaaag cgggctattc cctgcgcgat gagcaggccg ccgacgagcc acaagcatcg
    39181 cgcctgaaag agaatctgcc gctgattacg cttatcgtga tgatggcaat cagctggggt
    39241 ctggagcagt tcaatcatcc gttcgggcaa ctggcgttta tcgcgaccac gctagttggg
    39301 ctgtacccga ttgctcatca ggcattacgg ctgatcaaat ccggcagcta cttcgccatt
    39361 gaaaccttaa tgagcgtagc cgctattggt gcactgttta ttggcgcaac ggctgaagct
    39421 gcgatggtgt tgctgctgtt tttgattggt gaacgactgg aaggctgggc cgccagccgc
    39481 gcgcgtcagg gcgttagcgc gttaatggcg ctgaaaccag aaaccgccac gcgcctgcgt
    39541 aacggtgagc gggaagaggt ggcgattaac agcctgcggc ctggcgatgt gattgaagtc
    39601 gccgcaggtg ggcgtttgcc tgccgacggt aaactgctct caccgtttgc cagttttgat
    39661 gaaagcgccc taaccggaga atccattccg gtggagcgcg cgacgggcga taaagttcct
    39721 gcaggagcca ccagcgtaga ccgtctggta acgctggaag tgctgtcaga accgggtgcc
    39781 agcgccattg accggattct gaaactgatc gaagaagccg aagagcgccg cgcccccatt
    39841 gagcggttta tcgaccgttt cagccgtatt tatacgccag cgattatggc cgtcgccctg
    39901 ctggtaacgc tagtgccgcc gctgctgttt gccgccagct ggcaggagtg gatttataaa
    39961 ggtctgacgc tgctgctgat tggttgcccg tgtgcgttag ttatctcaac gcctgcggcg
    40021 ataacctccg ggctggcagc agcagcgcgt cgtggggcgt taattaaagg cggtgcggcg
    40081 ctggaacaac taggtcgtgt tacccaagtg gcgtttgata aaaccggtac gctgaccgtc
    40141 ggtaaaccgc gcgttaccgc gattcatccg gcaacgggta ttagtgaatc tgaactgctg
    40201 acactggcgg cggcggtcga gcaaggcgcg acgcatccac tggcgcaagc catcgtacgc
    40261 gaagcacagg ttgctgaact cgccattccc accgccgaat cacagcgggc actggtcggg
    40321 tctggcattg aagcgcaggt taatggtgag cgcgtgttga tatgcgctgc cgggaaacat
    40381 cccgctgatg catttgctgg tttgattaat gaactggaaa gcgccgggca aacggttgtg
    40441 ctggtagtac gtaatgatga cgtgctgggt atcattgcat tgcaggatac cctgcgcgcc
    40501 gacgctacaa ctgccatcag tgaactgaac gcgctggggg ttaaaggagt gatcctcacc
    40561 ggcgataatc cacgcgcagc ggcggcaatt gccggggagc tggggctgga gtttaaagcg
    40621 ggcctattgc cggaagataa agtcaaagcg gtgaccaagc tgaatcaaca tgcgcccctg
    40681 gcgatggtcg gtgatggtat taacgacgcg ccagcgatga aagctgccgc catcgggatt
    40741 gctatgggta gcggcacaga cgtggcgctg gaaaccgccg acgcagcatt aacccataac
    40801 cacctgcgcg gcctggtgca aatgattgaa ctggcacgcg ccactcacgc caatatccgc
    40861 cagaacatca ccattgcgct gggactgaaa gggatcttcc tcgtcaccac gcttttaggg
    40921 atgaccgggt tgtggctggc agtgctggca gatacgggtg cgacggtgct ggtgacggcg
    40981 aatgcgttaa ggttgttgcg caggagataa ggcaaaccga tcgcaacatt gagcgcgatc
    41041 ggtcccctcg cccctctggg gagagggtta gggggtgagg aaaaggcggc atcgaagcca
    41101 atcagcccct atcaaccgcc tttacgaatc aaataacgat aaggcagtcc atccgtctct
    41161 ttcgcaacca gttcgtgttc cataaaggta caaaacccag gaatatcgcg ggtagtggcc
    41221 ggatcgtcgg cgataatcag caacgtttcg ccaggctgca tattgcgcac ggttttgcgc
    41281 accatcatca ccggttccgg gcagcgcagg ccaagcgcgt cgagtgtgtg gtcagggctg
    41341 gaaaagagat cggtcatttt cttctcatca cttaaaaaaa cggcgctagt ttacgccctg
    41401 tgagtccgta agcaaaccag gttaacgatt gcgtgaaaat taaccattgc attgtcaacg
    41461 taaagcagta tcatgcggcg gctcgaaaaa agggtaagca cgttattatg ttaaggtaac
    41521 agacgtgtcg tacgtattgg gttccctcac cccaatggtt aatcaaaaag gtacaatatg
    41581 aacgttttct cgcaaactca acgctataag gcgttgttct ggttatcgtt atttcatctt
    41641 ctggtgatca cctccagtaa ctatctggtt cagcttcccg tctccatttt tggtttccat
    41701 accacctggg gcgcgtttag ctttccgttt atttttcttg ctaccgacct gaccgtgcgt
    41761 atttttggcg caccgctggc ccgacgcatt atcttcgcgg taatgatccc tgcgttatta
    41821 atctcctacg tcatctcgtc gctattctat atgggttcct ggcagggatt cggcgcactc
    41881 gcccacttca acctgtttgt cgcccgtatc gccaccgcca gtttcatggc ctacgcgctg
    41941 gggcaaatcc tcgacgtgca cgtttttaac cgcctgcgtc agagtcgccg ctggtggctg
    42001 gcaccgacag cgtccacact gttcggtaac gtcagcgaca cgctggcctt tttcttcatt
    42061 gccttctggc gtagcccgga tgcctttatg gctgaacact ggatggaaat cgcgctggtc
    42121 gattactgtt tcaaagtgtt aatcagtatc gttttcttcc tgccaatgta tggcgtatta
    42181 ctcaatatgc tgttgaaaag actggcagat aaatccgaaa tcaacgcttt gcaggcgagt
    42241 taaaggttcg ttatcagagt tgtgataaga tggatgaatg agccgttatg gccgtttatc
    42301 gaaaggaaga agtcaatgcg caatctggtt aaatatgtcg gaattggcct gctggttatg
    42361 gggcttgcgg cctgtgatga taaagacact aacgctacgg cgcagggttc ggtcgcggaa
    42421 agtaacgcta ccgggaatcc cgtcaacctg cttgatggca agttaagttt ctcgctgcca
    42481 gcggatatga ccgaccagag cggtaagctg ggaacgcagg ccaataacat gcatgtctgg
    42541 tccgacgcca ccgggcagaa agcagtcatc gtcatcatgg gcgatgatcc gaaagaagat
    42601 ctggcggtgt tggcgaagcg tctggaagat cagcaacgta gccgcgatcc gcagctgcaa
    42661 gtggtaacca ataaagccat tgagctgaaa ggtcacaaaa tgcagcagtt agacagtatt
    42721 atctccgcga aaggccagac ggcgtactct tccgttattc tgggtaacgt gggtaatcaa
    42781 ctgctgacca tgcaaattac gctgccagct gacgatcagc aaaaagcgca aaccaccgca
    42841 gaaaacatca ttaatacgct ggttattcag taagttttaa gatgatgagg cggcctcagg
    42901 gacgtgttcc ggaggccgtt tttttaatcg ccacgtcagt aataacgcga ttgcgaccag
    42961 tcccgccgcc gccagataaa tcaccggtac gcccgcccag ctcatcacca gcccagccag
    43021 tggtccagtc acgccaagcg ataaatccat aaatacggtg taagttgcca gcgccgcccc
    43081 ctaattttgc tgcggaaccg cttttaccgc cactacaccc aatgccggga acaccagcga
    43141 aaacccggcc cccgccagta agacgccgat tttcgccatc cacggcatag tcgccacgcc
    43201 aaccagtagc aggccgatta tctcaacgct aaagcaaatc atcgctacgt ttaagccacc
    43261 gatacggtta atgccgttag ggaataacaa acgcgtaccg acaaacgcac agctaaacag
    43321 cgtcagcgcg aaagccgcac cgtcccaacc tttagcgtca taaaacagcg tgataaaggt
    43381 ggcgatgacg ccaaatccgg cggaagccag tgccagcgcc ataccgtaca gccagacgcg
    43441 cccaagcacc gcgcgaaacg gcagcggttt gcctttactg gcttttaccg tcggacgcgg
    43501 gatcgccaac aaaatggcca ccagcgccac gcccataatg attaacgcta acgcctgcaa
    43561 gccgccccag tgataaaaca cgacgcctaa cggcgcaccc atcgccatcg ccccgtaagt
    43621 gacaatgccg ttccacgaaa tcacccgccc gatatgcagc gagccaacca ctccaacgcc
    43681 ccatagggtc gatcccgttc cggcaaaact ttgcccaatc ccaaggatga cgcgccccag
    43741 gcaaagtaat aacaggctga tgacaggcag actggcggtt aatcctgccg tcagataccc
    43801 cagaccgctc aaaaagcagc cgcataaacc gaagacgaca atctttttgg gtcccagcaa
    43861 atcggcgtaa cgtccggcat gagggcggct cagcaaggtg gcgaaatatt gcaggctgat
    43921 aaccaatcct gcccagaagg cgctaaagcc catcacatca tggacatagc ccggtaatac
    43981 agcgagcggc aacccgatgg tgaggtagct ggcgaagtta aacatgacaa tggagacaat
    44041 gcgcaaattc aggcgcaatc cgtttagcgc gggttcggct acgggttcgg gcatgaggat
    44101 caccacattt ttacaacagt gtttcatttt taccatgtgc tgacgtgaaa atcagcagta
    44161 agaatcagaa tattgctggc gtgactcccg ctacacttaa cacaaaaagt cacaaggaag
    44221 ccccaatgga aacccctcaa cccgataaaa cgggcatgca cattctgctc aagttggcct
    44281 cgctggtagt gatcctcgcg ggcattcacg cagcggcaga tatcattgtg cagctgttac
    44341 tggcgctgtt ttttgccatc gtcctcaacc cgctcgttac ctggtttatt cgtcggggag
    44401 tacaacgccc cgttgccatt acgattgtag tggtggtgat gctgatcgca ctaaccgcgc
    44461 tggtcggcgt actggcggca tcgtttaacg aatttatctc tatgctgccg aagtttaata
    44521 aggagctgac gcgcaaactt tttaaattgc aggagatgtt gccttttctt tatttgcata
    44581 tgtcgccgga gcgaatgctg cagcggatgg actcggaaaa agtggttacc ttcaccacag
    44641 cgctaatgac cgggctttcc ggggcaatgg cgagcgtgct tttgctggtg atgaccgtag
    44701 tttttatgct gtttgaagtg cgccacgtcc cttacaaaat gcgttttgcg ctgaataacc
    44761 cacagattca catcgcagga ttacaccgcg cacttaaagg cgtttcgcat tatctggcgt
    44821 taaagacgct actcagttta tggacaggtg tcatcgtctg gctggggctg gcgctaatgg
    44881 gcgtacagtt tgcgctgatg tgggcagtac tggcgttttt gctcaactac gtgcccaata
    44941 tcggcgcggt aatttccgcc gtaccgccaa tgattcaggt gctgctgttt aatggtgttt
    45001 acgaatgtat tctggtcggc gcattgtttt tagtggtcca tatggtcatc ggcaatattt
    45061 tagaaccacg gatgatgggc catcgcctgg ggatgtccac catggtggta tttctttcat
    45121 tgttaatctg gggatggctg ctcggcccgg tagggatgct actttcggtg ccattaacca
    45181 gagtgtgtaa aatctggatg gaaacgacga aaggtggcag caaactggcg attttactgg
    45241 ggccggacag accgaaaagt cggttaccgg gatgaggcga caagtgatac gatacgcact
    45301 ttcattttcc attaaacgtt ggccctgata tgtatcggat agttctgggg aaagtttcga
    45361 ccttaagcgc agctccactg ccaccgggtt tacgcgagca agcaccgcaa ggtccacgac
    45421 gcgaacgctg gctggcgggg cgtgcattgc tttcgcacac gctttccccg ctaccggaga
    45481 tcatctatgg cgaacaaggc aaacctgcat tcgcgccgga aacgccgcta tggttcaact
    45541 taagccatag cggtgacgat atcgctctgc tgttgagcga tgaaggcgaa gtcggctgcg
    45601 atatcgaagt gattcgcccg cgcgccaact ggcgctggct ggcgaacgcc gtgttcagcc
    45661 tcggggaaca cgctgaaatg gacgccgtgc atcctgatca gcaactggaa atgttctggc
    45721 gcatctggac gcgcaaagaa gccatcgtta aacagcgtgg cggcagcgcc tggcaaatcg
    45781 tcagcgtaga tagcacctat cactcctcgc tgtcagtcag ccattgtcag ctagaaaatt
    45841 taagccttgc gatctgcacc cccaccccct ttacgctcac cgccgacagt gtgcaatgga
    45901 tcgattcagt taactgatcc gcccacccga ctgcccatct attgatccag aacaggtaat
    45961 cagtatgacg aatacttaaa atcgtcatac ttatttccgc catctatttt aatccattgg
    46021 ggttaccatg ctctccacac tccgccgcac tctatttgcg ctgctggctt gtgcgtcttt
    46081 tatcgtccat gccgctgcac cagatgaaat caccaccgcc tggccggtga atgtcgggcc
    46141 actaaacccg cacctttaca cgcctaacca gatgttcgcc cagagcatgg tttatgaacc
    46201 attggtgaaa tatcaggcag acggttcggt gatcccgtgg ctggcaaaaa gctggactca
    46261 ttcagaagat ggtaaaacct ggaccttcac cctgcgtgat gacgtgaaat tctccaacgg
    46321 tgaaccgttc gatgccgagg cggcggcaga aaacttccgc gcagtgctcg ataaccgtca
    46381 acgtcacgcc tggctggagc tggcaaacca gattgttgat gttaaagcac tcaataaaac
    46441 agagctgcaa attaccctga aaagcgccta ctatcctttc ctgcaagaac tggccctgcc
    46501 ccgtcctttc cgttttatcg ctccctcgca gtttaaaaac catgaaacca tgaacggaat
    46561 taaagcgccg attggcaccg gaccgtggat tttgcaggaa tcgaaactga atcagtacga
    46621 tgtcttcgtc cgtaacgaaa actactgggg cgaaaagcca gcgattaaaa agatcacctt
    46681 taacgtcatc ccggacccga ctacccgcgc ggtggcgttt gaaactggcg atatcgacct
    46741 gctgtacgga aacgaagggt tattaccgct cgataccttc gcccgcttta gccaaaatcc
    46801 ggcttaccac acccaactgt cacagccgct cgaaaccgtg atgctggcgc tcaataccgc
    46861 caaagccccc accaacgagc tggcagtacg tgaagctctt aattacgcgg taaacaaaaa
    46921 atcgctgatt gataacgcgt tatatggcac ccagcaggtc gccgacaccc tgtttgcccc
    46981 ttctgtgcct tacgctaatc tcggcctgaa accgcgccag tacgatccgc agaaagcgaa
    47041 agagttgctg gaaaaagccg gttggacgct gcctgcgggc aaagacatcc gcgagaaaaa
    47101 tggtcagccg ctgcgcattg aactgtcgtt catcggcacc gatgcgttaa gcaaatcgat
    47161 ggcggaaatc attcaggctg atatgcgcca gattggcgca gatgtctcgc tgattggcga
    47221 agaagagagc agtatctatg ctcgtcagcg cgacggtcgt tttggcatga ttttccaccg
    47281 cacctggggc gcgccatacg atccacacgc cttcctgagt tcaatgcgcg taccgtcgca
    47341 cgctgacttc caggcacagc aaggattagc cgacaagccg ctgattgata aagagatcgg
    47401 cgaagtgctg gcgacccatg acgaaacgca acgtcaggcg ctgtatcgcg acattctgac
    47461 ccgtctgcat gacgaggcgg tttatctgcc tatcagctac atctcgatga tggtggtatc
    47521 aaaaccggag ctgggtaata tcccctacgc gccgatcgcc accgaaattc cgttcgaaca
    47581 gattaaaccg gtgaaacctt aatgttgcgt tacgtattac gccgctttct gctgctgatc
    47641 ccgatggtgc tcgccgcctc ggtgatcatt tttctgatgc tgcgcctcgg taccggcgac
    47701 ccggcgctcg attatctgcg tttatctaac ctgccgccga cgccggagat gctggcctct
    47761 acccgcacca tgctcgggct ggatcagccg ctgtacgtcc agtacggtac ctggttgtgg
    47821 aaggcgctgc atcttgactt tggtatctca ttcgccagcc aacgcccggt actggacgat
    47881 atgctgaact tcctgcccac cacgctggaa cttgcaggtg cggcgctggt attaattctg
    47941 ctcacttccg taccgctcgg tatctgggcg gcgcgccacc gcgatcgttt gccggatttc
    48001 gccgtgcgct ttatcgcgtt tcttggcgtg tcgatgccca acttctggct ggcatttttg
    48061 ctagtgatgg cgttttcggt gtatctgcaa tggctacccg cgatgggcta cggcagctgg
    48121 cagcacatca ttttacctgc ggtttccatc gcctttatgt cgctggcgat taacgcgcga
    48181 ctgctgcgcg ccagtatgct ggacgtcgcc ggtcagcgtc acgtcacctg ggcgcggcta
    48241 cgcggcctga atgacaaaca gaccgaacgc cgccatattc tgcgcaatgc ctcgctgccg
    48301 atgatcaccg ccgtggggat gcacattggc gaattgattg gcgggacgat gattatcgaa
    48361 aacatctttg cctggcctgg cgtcgggcgc tatgcggtgt cggcgatttt taaccgtgac
    48421 tatccggtga ttcagtgctt tacgctgatg atggtggtgg tttttgtggt ctgtaatttg
    48481 attgtcgatt tgctcaacgc cgcgctggac ccgcgcattc gtcgtcatga aggagcgcac
    48541 gcgtgaactt tttcctctct tcccgctggt cggtacgcct ggcgctgatc attatcgccc
    48601 tgctggcgct gattgcgctc accagccagt ggtggctgcc gtacgatcca caggcaattg
    48661 atttgccgtc gtgcctgctt tcgccggatg cgcagcactg gctgggcacc gatcacttag
    48721 gtcgcgatat tttctcgcgg ctgatggcag cgacccgcgt gtcgctcggt tcggtaatgg
    48781 cctgcctgct gctggtgctg acattagggc tggttattgg cggcagcgcc gggttgattg
    48841 gcgggcgcgt tgatcaggcc accatgcgcg tcgccgatat gtttatgacc ttcccgacct
    48901 cgattctgtc gttctttatg gttggcgtgc tcggcaccgg gctgaccaac gtaattatcg
    48961 ccatcgccct gtcgcactgg gcgtggtatg cacgcatggt gcgcagcttg gtgatttcac
    49021 tacgccaacg cgagtttgtg ctggcgtcac ggctttccgg tgcgggccat gtgcgggtgt
    49081 ttgtcgatca tctggcaggc gcggtgatcc cttcgctgct ggtgctggca acgctggata
    49141 tcggccatat gatgctgcac gtcgcgggga tgtctttcct tggcctcggt gtgaccgcgc
    49201 cgaccgccga atggggcgtg atgattaacg acgcgcgcca gtatatctgg acccagccgc
    49261 tgcaaatgtt ctggccgggg ctggcgctgt ttatcagcgt gatggccttt aacctggtgg
    49321 gtgacgcact gcgcgatcat ctggatcctc atctggtgac ggagcacgca cactaatgcc
    49381 gcaacagatt gaactacgta atatcgcgct acaggccgcg cagccgctgg tacacggtgt
    49441 atcgttaacc ctgcaacgcg ggcgcgtgct ggcgttagtc ggcggtagcg gcagcgggaa
    49501 atcattaacc tgcgccgcga cgctgggcat tttgcccgct ggcgttcgcc agacggcggg
    49561 ggaaatttta gccgatggca aaccggtttc gccttacgcc ctgcgcggca tcaaaattgc
    49621 caccatcatg cagaacccgc gcagcgcctt taatccactg cacaccatgc acacccacgc
    49681 gcgggaaacc tgcctggcgt tagggaaacc cgccgatgac gctacgctta ccgctgccat
    49741 agaagcggtg gggctggaaa acgccgcgcg cgtgctgaag ctgtacccgt tcgagatgag
    49801 cggcggcatg ttgcagcgca tgatgattgc gatggcggtg ctgtgtgaat caccgtttat
    49861 catcgccgat gaaccgacca ccgacctcga cgtggtagca caggcgcgca tcctcgatct
    49921 gctggaaagc attatgcaaa aacaagcgcc gggaatgctg ctggtcaccc atgatatggg
    49981 cgttgtggcg cgtctggcgg atgacgtggc ggtgatgtca caaggtaaaa ttgtcgaaca
    50041 gggcgatgta gaaacgctgt ttaacgcccc caaacatacg gtgacgcgca gcctggtttc
    50101 cgctcatctc gccctctacg gtatggagct ggcatcatga ccttacttaa cgtctgcggc
    50161 ctttcccatc actatgccca cggtggattt agcggaaaac atcaacatca ggcggtgctg
    50221 aataacgttt ccctgaccct gaaaagcggc gaaaccgtcg ccctgctggg acgcagcggc
    50281 tgcggaaaaa gcaccctcgc gcggctgctg gtaggcttag agtctccctc acaagggaat
    50341 attagctggc gcggcgaatc gctggcgaaa cttaatcgcg cccaacgtaa agccttccgc
    50401 cgtgatattc agatggtgtt tcaggactcc atcagtgccg tgaatccgcg caaaaccgtg
    50461 cgcgagatcc tgcgtgaacc gatgcgccac ctgctatcac tgaaaaaatc cgaacaactg
    50521 gcgcgcgcca gcgaaatgct gaaggcggtc gatctcgatg acagcgttct cgacaaacgc
    50581 ccaccgcagt taagcggcgg ccagctccag ctcgtctgcc tggcacgcgc gctggcggtc
    50641 gaaccgaaat tgctgattct ggatgaagcc gtttctaacc ttgatctcgt gttacaggcg
    50701 ggcgtcattc gcctgctgaa aaatctacaa caacagtttg gcactgcctg cctgttcatc
    50761 actcatgact tacgcctggt ggaacgcttt tgccagcgag tgatggtgat ggacaacgga
    50821 caaatcgccg aaacccaggc ggtgggagat aaattaacct tttcctctga cgccggacgt
    50881 gtgctacaaa acgcggtatt acccgcattc cccgtgcgcc gtcgcacctc agaaaaggtt
    50941 taacgccaat gcaacgagtc accatcacgc ttgatgacga tttactggag acgctggaca
    51001 gcctgagcca gcgtcgtggt tataacaacc gttccgaagc tatccgcgat attctgcgta
    51061 gtgccctggc gcaggaagct acccagcagc acggcacaca aggtttcgcg gtgctgtcgt
    51121 atgtgtatga acacgaaaaa cgcgacttag ccagccgtat tgtttccacc cagcatcatc
    51181 accacgacct ctccgtcgcc acgctacatg tgcacatcaa ccacgacgac tgtctggaaa
    51241 tcgccgtgtt gaaaggtgac atgggtgacg tgcagcattt tgccgatgac gttatcgccc
    51301 agcgcggcgt gcggcacggg catttgcagt gcctgccgaa ggaagattga gttatttcta
    51361 aaacaaggaa agaaaagata ttaactgaac ttaatttata aaattaagtt cagttgcata
    51421 gatagatcct gtaaaggcag atattctgcg taatgtacgt cgctaaaatt tataaatgat
    51481 tcaaacgctc attcagtgtt tcaataatat aggtattaac agagacttgt tgctgcgcag
    51541 ccgcattatt aaggcgttca ctcagcgatt ctggatagcg taacgtaaag gtcttaatct
    51601 tttctgtacg cgcatacggc tcaatacccg cagctttaca atcctcaaga tactctcgca
    51661 gggaaagctc tccttctttt tgcagcccct gaatactgtc agagacaaaa tcacaataac
    51721 ctgacaagcc caggaatttg ccacgaaatg cattcagttc tggcacgtag gtaataacag
    51781 ctggttgccc ggcaatctcc atagagtttg gcgtcttcag tttgatcatg gttttacccc
    51841 tatacttagt aaccagtcac gcacgctttc tacagcgcct ttatcggtat ctggcgacgg
    51901 atgaggccga tggaaacacg caacgctcat atttagtatg aacttgcaac gcgaacctct
    51961 tccttcctta atttctccgc ctaatgcttt aaccagtgac tctatatcag accacttgat
    52021 cccttgagga acaggtgttt taaatatttg atccaatgta tttttttgtt tcttccgtaa
    52081 agcctgtacc ttggttctca tggcatgtcc ttatgacttc actttatgac atcacagtat
    52141 gaagtcaagt ttcccttcct tacgccattg tcccaatcgt cttcctgaat cgcagcagtg
    52201 cgatggtgaa aaacgcgccg ccaattgcca tcagcgtcag gaactgcggc cagacgattt
    52261 cgaatccggc accccggtag aggatggcct gcgcgaggct aacaaagtgt gtcgtcggca
    52321 tggtcagcat aatgtcctgc accatctgcg gcatactttc gcgcggcgtg gatccaccgg
    52381 aaagcatttg cagcggcagc agcaccagaa tcaccagcag ccccagttgc ggcattgaac
    52441 gcgctatcgt ccccataaaa atgccgattg acgtggtggc aaacagactg agcgccacgc
    52501 ccagcataaa cagcgggatc gagccttcaa tcggtacgcc cagtacacct ttcaccatca
    52561 gcaccagcga taatcccgat accaccagca ccaccagccc catcgaccag atcttcgcca
    52621 tcatgatctc aaacggcgtt atcggcatca ccagtaaatg ttccaccgtg ccgtgttcac
    52681 gctcgcggat cagcgccgat ccggtcaata caatcgccag catggtaatg ttgttgatga
    52741 tcgccatcac cccgccaaac cacgcgggat caaggttcgg gttaaagcgc atccgggttt
    52801 ccagcgatac caacggttcg ctgttatcac ggtagcgcgc gacaaagctg ttcacttcac
    52861 cgttgataat attctggata tacccattgc cggtaaatgc ctggctcatg cgcgtggcat
    52921 cgacgttcac ctgaatatcc ggctggcgtc cggcgaggac atcacgctga aaattaggcg
    52981 gaatatttat cgcgaaggta tagcgtccgg cgtccagtcc ggcatccatc tcatcggcgg
    53041 tgatcatctc cggtggcaaa aaccacggac gatagaagct gttaacgatc cggttcgata
    53101 actgcgattg atccatatcg gcaatggcga tcggcgcgag gttcaacgat cctggcgtaa
    53161 cggtcgctga cgaatacacc gacaccgtaa acgagaagac aatcagcgtc agcatcgctt
    53221 tatcaccgag cagactgcgc aactctttga tacccagatt aaaaatattg cgtaaatggc
    53281 gcatcatccc tcctgttttt tcagcagcag gatgcttaag cccatcacca gcgggatggc
    53341 tatcagcaac gggataaaaa gttgccacaa atcagtcaga tccagcgctt tcgagaacgt
    53401 tccgcgggcg atagtcagaa aatgactggt cgggtaaacc tcgccgatcc aacgtccagg
    53461 cccttccagc gaagctaccg gatcgatcat cccggaaaac tgtgtcgccg ggatcaatgt
    53521 gataatcgcc gttccgaaaa tggcggcaat ctggcttttc ataaaggtgg agatcagcag
    53581 ccccattcca gtggcaatga tgatatacag cagcgccgcc aaggtgagcg tcaggaaact
    53641 gcctttatgc ggtacgccaa acacaaacac cgacaggccg cagagcagga aaaagttcag
    53701 catccccagc gcgatgtacg gcagctgttt gccaagcaaa aattcgctac gggtggtggg
    53761 ggtcacgtaa aggttgataa tcgaccctag ctctttttcc cgcaccacgc taagggcgct
    53821 tagcattgac gggatcatca tcagcagaag cgggatcacc gccggaacaa tcgctggcag
    53881 gctttttacg tccgggttat agcgataacg cgtctcaatg ttcatcagcc cgctttggct
    53941 ggcgggtgtc aattgtcggc tcgccacatc ctgaagccaa ctctggtgca ttgcctgcac
    54001 gtaacctttt accgtttcgg cacgactggg catcgctccg tcgatccaga cgccgagttc
    54061 cacaggcgta ccacgcgcga tatcgcgccc gaaattgggc gggatctcaa tggccaccgt
    54121 gatatcgccc gcacgcatcc gacgatcaag ctcgtcataa ctggtgagcg gcggctgttc
    54181 gataaagtaa cgggaaccgg agaggttgag tgtccacgcc tggctactga cggtctggtc
    54241 gcggtcgagc accgcaaagc gcaggttttc cacatccata ctgatgccgt aacccattat
    54301 cagcatcagg atcaccgttc ccatcagcgc cagcgtcgaa cgtactggat cgcgtcgcag
    54361 ttccagcgct tcgcggcggc tgtagctaaa cagacggcgc aggctaaatc cctgacgcgg
    54421 cgcgtgggtg gtgtcgtgta ccacgggcgg cgcttcggct tcgttgctct gccctgccgc
    54481 ttcctgcaaa taggcgataa atgcctcttc cagactggcg gctccgcgtt tctcaaccag
    54541 ttcctgcggt gtaccgctgg caagcacttt tccggcgtgc atcagtgaga tgcggtcgca
    54601 acgttccgct tcgttcataa agtgggtgga gatgaagata gtcactttgt cctggcgcga
    54661 gagatcgacc atcaactgcc agaacatatc cctcgccacc ggatcgacac cagaagtagg
    54721 ctcatcgagg attaacatct ccgggcgatg aatcaccgcc accgccagcg aaagccgctg
    54781 gcgaatgccg agcggcaatg actccggcag aacatcttca acgtcgttga gcttaaaacg
    54841 ctcgctcatt tcagccactc gcgcgggaat ttccgcttcc gggatgtgaa acaaacgggc
    54901 atgtaactcc aggttttgcc gcacggtgag ttcgttatag agcgaaaacg actgcgacat
    54961 atagcccaca cgacggcggg tatcgatatc ttttggatca accggttgcc caaatagcca
    55021 cgcctcacct tcgctggcgg gcagcagtcc ggtaagcatt ttcatggtgg tggatttacc
    55081 gcagccgttc gaaccaagga atccaaaaat ctccccgcgt ggaatgcgga aattgacgtg
    55141 atcaacggca acgaaggaac caaaacgcat ggtcagatcg cgcgcttcga tggcaatctc
    55201 tgcgttttca ggttgatacg gtgggatcac taccgcctga tgcgcctggc gttgcgcttg
    55261 cggtaacaga ttaataaatg cttcttccag cgtggcgctt tgcgtttgct gccgcagctc
    55321 ttcggcgctg ccggttgcca gcacttctcc ggcattcatc gctaccagcc agtcgaagcg
    55381 ttcggcctct tccatatagg cggtggcgac cagcacgctc atattgctct gccgctggcg
    55441 aatactgtcg atcagatccc agaactgggc gcgggagagc gggtcaaccc ccgttgttgg
    55501 ctcatcaagg atcaacagtt ccgggtcgtg gattaacgcg cagcacagcc caagtttttg
    55561 cttcatcccg ccggagagtt tacctgccgg acgatcgcga aacggtgcta acccggtgct
    55621 ggtcagcagc tcattaattc gcacttcccg ctccgcttta tcgtgaccga acaggcgagc
    55681 gaaaaaatcg acattttcat acaccgacaa ggtgtggtag aggtttttgc ccagcccctg
    55741 cggcatccag gcgatgcgcg ggcagacgtc tcggcgatgc ttcgggtcgt gcatatcgcc
    55801 acccagcacc accacattgc cctgctcaat gacgcgggca ccggaaatca gtgacaataa
    55861 actcgacttc ccgacgccat ccggaccaat cagcccgacc atgcaacggg ccggaatatc
    55921 gagagtgata ttgttcagcg caacggtttt tccataatgc tggctcacgc ccgccagttg
    55981 cgcgacaggc gggacaggaa ccagttccag atgcgtcatt gcggcaacct caccacgagg
    56041 tcgtcaggcc acggaagttc ttcattcacc cgcacccacg ctacgcccgg taaaccggtt
    56101 ttgacatatt ccagatgctg ctggagtaat tccggtggga tacgcgcttt gacgcggaac
    56161 atcagtttca gccgttcatc gctggtttcg acggtttttg gcgtgaactg ggcgacactg
    56221 gcgacaaaac tgatggttgc aggaatacgc agatctggcg cggcatcgag gatcagccgg
    56281 gcttcaccgc ccagtttcag cgtgcccgcc tgttcggttg gcaggaagaa ggtcatatag
    56341 acgtcgctga gatcgaccat attcagcacc cgaccgcctg ccgccagcac ttcccctggc
    56401 tcggcaaccc gatactgcac gcgtccgtca cgcggggctt tcagttcact gtcatcgata
    56461 tctgcggcaa tgcgccgttc agtggcttgc gctgcttcga cccgagtttg cgcctgaatg
    56521 atattggtgc gtgctgcttc tatagccgct ttagaagccg aaacctgggc tttcgctgat
    56581 tccagcgcag ctcgggcgct ctcagcggcg gcgcggtcgt catccagctg ttgcgcagaa
    56641 atcgcccctc gttgggcgag cgagcgggaa cgcgtatgac gtttggcaac agagtccagt
    56701 tcggcctggc gttgattgac cagcgactgt gcggcacgag tttcactttg tcgttgctcc
    56761 agcaaagcct gcgcggcggc aacggcgctt tgtgcctctt tgatttgcgc gatggcttcc
    56821 agtcgctgtt cctgcaacac gcgagtatcc atcttcgcca gcacttcgcc ttcgcgaaca
    56881 aactggcctt ctttcaccag aatggtgtcg atacgcccgg cagttttgct ggcgatatcc
    56941 acttccgtcg cttcaatgcg cccattgctg acagcaaagc cttccggcac acccgccggg
    57001 cgcaacagcc accacgccac gacggcagcc accgccagtg ccccgacaac ccaccacgcc
    57061 agatggcgct tactcttatc cataatcgac ccgccataat ccctgtaagc aaacgacgcg
    57121 tgttacaact gccgccgaac gacgttcagc ggattcttcc atccgggata agaaggaaac
    57181 gacaaatacg accagcgcgc agagcattga gcgccaacgg ggaaggctta gcgaaaacgg
    57241 gtagtaatgt tgtcatcatc agcattcctg gccgtaaatg aaacacgcat actcataagt
    57301 gtgatctata aaactggctg acaaaacggt atccatcctg ttggatcgcg tttcacgcct
    57361 gttcagttgc aggaacagag cattcctttt tacgttttat caacagtctc aaccagatag
    57421 taacattaat attcatgttg tctatggttc agagctgtaa cattgtgcgc cttctaatca
    57481 aaggcataag tccatttctg tttttttcat gctggtaaga cttccaggga gtggttatct
    57541 ctggccctgc accaaggtta atttggcaat tatgcatgct gaacattctt ccagcatgtt
    57601 tactgtcacg ggcgttaaga cctaaaactg tccccgtttc acctatgcgt tctctcccac
    57661 catttccgtt atgtgcaaca acttgattta tacctaacat ttgtgaacat gctaatagtg
    57721 cttcaagttt aggtctgtaa tctgtcgaaa cagaaaaata acgattaaag gatcgacaag
    57781 agatagcccg gctagtttta aactttttgc cattaattat agcatgatgc tttttattaa
    57841 taatttccgc taattcgacc ggatcgagag cattggtcat ttcatcaata gagacagtta
    57901 tcggtcctat aatgtaacgt tttcgctgat catcaaaagt tatcccatga tgatttgcca
    57961 ttattttgta tttggggtca tagaaacata caggataatc ttttatcccg tcgtaggtat
    58021 cctcatcaag tggtttcatg cgcgtgaagt tttgcatata gttaccattg caattcgttt
    58081 catgatttcc agctaataca tgaacatttt cattgatact cattatgcgt tttaacaaat
    58141 cgatgataaa ttgaacgcca cagatattac taaatcgatc gccggtttgg tcgccaagaa
    58201 acgtacatgg cgtgactgcg tctttattaa taaccaaata aggctgaaga ctttctaaaa
    58261 cattatctct tctgttcagt atactgatta ttgtattaac attagtgtca agttgctcat
    58321 taaagttctc ccttagcaat acgttgagaa cttcatcttc tatattaagc aactggctaa
    58381 ggaaattttc tccttcagcc atgattccca aatgtcctgt tgcaaaaagc gcgtacaaaa
    58441 ctgcaccgac tgagctatcg gtatcgccaa aataggtcgg cctggtactt gtgggatcaa
    58501 caggtgaaca attggagaaa taggttctgt cagcgaactg ggattttata attgtttcga
    58561 atttaatatc aaatacattg tattgcgata tttcccagtt ccagatgggt ttgtacgact
    58621 cagtaactgc aacatcatta gcattatcat ttactgtcat attattaagt aaataaacaa
    58681 tgctgcacca attaatatcg gtattatcaa gagggcaacc gtgattttta ctattaattg
    58741 tcacaataaa ctgataacag ttattcctac gtagaatatg agcgcgcatg cccgatagca
    58801 tactatattt ttctgaatga ttcgtttgta tggtaaatga aattttgtca tcttgaatag
    58861 attgttggaa acgttctatt tgccaatcat caattttttc cacaacttca ccgagcattg
    58921 tcctggcaat acttgatata tcatcaccta taccatttgc aatataccca aacagttctt
    58981 tagtgaagtt ggtaacatgc tcttgcacca tatcatttgt tgcgattgtc gttgtactgc
    59041 cattagtgcc tgccacagtt ccaattttca tcttttacct catattattc accgcgagga
    59101 atacatagtt aacattttat cttatgacat acacctcata ttcgatggga aaataattat
    59161 attttgcaca aatcattcag ccttaaagca taatatgaaa tttcttatct catagcagag
    59221 aaagtcccta aagattgtag aggcgtcatc agtacatacc tctacaatcg attaagaaaa
    59281 cctatgaaat actggcaaaa ttaaaaagag ataccacagc actcatgaag ttttattgag
    59341 gttgaaaaca actaagccct gggacacctt ttttatcacc acgttcgtta ggtgctatct
    59401 tccaagaatt tagccccatg gtataattat caattttttg atgctggtct acccctaatc
    59461 cataatggcc aa
//
LOCUS       DADWTH010000014        59445 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.014, whole genome shotgun sequence.
ACCESSION   DADWTH010000014 DADWTH010000000
VERSION     DADWTH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.014"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            545..1834
                     /gene="sdaC"
                     /locus_tag="JNY02_000856"
     CDS             545..1834
                     /gene="sdaC"
                     /locus_tag="JNY02_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBB9432497.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLSMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1892..3259
                     /gene="sdaB"
                     /locus_tag="JNY02_000857"
     CDS             1892..3259
                     /gene="sdaB"
                     /locus_tag="JNY02_000857"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBB9432498.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3370..4125
                     /gene="xni"
                     /locus_tag="JNY02_000858"
     CDS             3370..4125
                     /gene="xni"
                     /locus_tag="JNY02_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBB9432499.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="JNY02_000859"
     CDS             complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="JNY02_000859"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBB9432500.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="JNY02_000860"
     CDS             complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="JNY02_000860"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBB9432501.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6548..7864
                     /gene="fucP"
                     /locus_tag="JNY02_000861"
     CDS             6548..7864
                     /gene="fucP"
                     /locus_tag="JNY02_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBB9432502.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7897..9672
                     /gene="fucI"
                     /locus_tag="JNY02_000862"
     CDS             7897..9672
                     /gene="fucI"
                     /locus_tag="JNY02_000862"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBB9432503.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9781..11200
                     /gene="fucK"
                     /locus_tag="JNY02_000863"
                     /pseudo
     CDS             9781..11200
                     /gene="fucK"
                     /locus_tag="JNY02_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="JNY02_000864"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="JNY02_000864"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBB9432504.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="JNY02_000865"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="JNY02_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBB9432505.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGAPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="JNY02_000866"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="JNY02_000866"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBB9432506.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="JNY02_000867"
     CDS             complement(13550..13945)
                     /locus_tag="JNY02_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBB9432507.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="JNY02_000868"
     CDS             complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="JNY02_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBB9432508.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKIPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15232..15459)
                     /locus_tag="JNY02_000869"
     CDS             complement(15232..15459)
                     /locus_tag="JNY02_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBB9432509.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15651..16856
                     /gene="csdA"
                     /locus_tag="JNY02_000870"
     CDS             15651..16856
                     /gene="csdA"
                     /locus_tag="JNY02_000870"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBB9432510.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16856..17299
                     /gene="csdE"
                     /locus_tag="JNY02_000871"
     CDS             16856..17299
                     /gene="csdE"
                     /locus_tag="JNY02_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBB9432511.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELELRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="JNY02_000872"
     CDS             complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="JNY02_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBB9432512.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="JNY02_000873"
     CDS             complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="JNY02_000873"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBB9432513.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19700..19776
                     /locus_tag="JNY02_000874"
     tRNA            19700..19776
                     /locus_tag="JNY02_000874"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19734..19736,aa:Met,seq:cat)
     gene            19810..19886
                     /locus_tag="JNY02_000875"
     tRNA            19810..19886
                     /locus_tag="JNY02_000875"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19844..19846,aa:Met,seq:cat)
     gene            complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="JNY02_000876"
     CDS             complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="JNY02_000876"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBB9432514.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21446..22777
                     /gene="argA"
                     /locus_tag="JNY02_000877"
     CDS             21446..22777
                     /gene="argA"
                     /locus_tag="JNY02_000877"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBB9432515.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22839..24665)
                     /gene="recD"
                     /locus_tag="JNY02_000878"
     CDS             complement(22839..24665)
                     /gene="recD"
                     /locus_tag="JNY02_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBB9432516.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMLL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24665..28207)
                     /gene="recB"
                     /locus_tag="JNY02_000879"
     CDS             complement(24665..28207)
                     /gene="recB"
                     /locus_tag="JNY02_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBB9432517.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="JNY02_000880"
     CDS             complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="JNY02_000880"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBB9432518.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31264..34632)
                     /gene="recC"
                     /locus_tag="JNY02_000881"
     CDS             complement(31264..34632)
                     /gene="recC"
                     /locus_tag="JNY02_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBB9432519.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPLWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34645..34968)
                     /locus_tag="JNY02_000882"
     CDS             complement(34645..34968)
                     /locus_tag="JNY02_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBB9432520.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34953..35360)
                     /locus_tag="JNY02_000883"
     CDS             complement(34953..35360)
                     /locus_tag="JNY02_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBB9432521.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35357..35920)
                     /locus_tag="JNY02_000884"
     CDS             complement(35357..35920)
                     /locus_tag="JNY02_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBB9432522.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35911..36381)
                     /locus_tag="JNY02_000885"
                     /pseudo
     CDS             complement(35911..36381)
                     /locus_tag="JNY02_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36565..37359)
                     /gene="thyA"
                     /locus_tag="JNY02_000886"
     CDS             complement(36565..37359)
                     /gene="thyA"
                     /locus_tag="JNY02_000886"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBB9432523.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37366..38241)
                     /gene="lgt"
                     /locus_tag="JNY02_000887"
     CDS             complement(37366..38241)
                     /gene="lgt"
                     /locus_tag="JNY02_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBB9432524.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38392..40638)
                     /gene="ptsP"
                     /locus_tag="JNY02_000888"
     CDS             complement(38392..40638)
                     /gene="ptsP"
                     /locus_tag="JNY02_000888"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBB9432525.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSI
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40651..41181)
                     /gene="rppH"
                     /locus_tag="JNY02_000889"
     CDS             complement(40651..41181)
                     /gene="rppH"
                     /locus_tag="JNY02_000889"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBB9432526.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41866..42555
                     /gene="mutH"
                     /locus_tag="JNY02_000890"
     CDS             41866..42555
                     /gene="mutH"
                     /locus_tag="JNY02_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBB9432527.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42624..43337
                     /locus_tag="JNY02_000891"
     CDS             42624..43337
                     /locus_tag="JNY02_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBB9432528.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43475..43693
                     /gene="ygdR"
                     /locus_tag="JNY02_000892"
     CDS             43475..43693
                     /gene="ygdR"
                     /locus_tag="JNY02_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBB9432529.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43801..44841
                     /locus_tag="JNY02_000893"
     CDS             43801..44841
                     /locus_tag="JNY02_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBB9432530.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44874..46067)
                     /gene="lplT"
                     /locus_tag="JNY02_000894"
     CDS             complement(44874..46067)
                     /gene="lplT"
                     /locus_tag="JNY02_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBB9432531.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FCPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACVLAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(46060..48219)
                     /gene="aas"
                     /locus_tag="JNY02_000895"
     CDS             complement(46060..48219)
                     /gene="aas"
                     /locus_tag="JNY02_000895"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBB9432532.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSVITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48608..49639
                     /gene="galR"
                     /locus_tag="JNY02_000896"
     CDS             48608..49639
                     /gene="galR"
                     /locus_tag="JNY02_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBB9432533.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49646..50908)
                     /gene="lysA"
                     /locus_tag="JNY02_000897"
     CDS             complement(49646..50908)
                     /gene="lysA"
                     /locus_tag="JNY02_000897"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBB9432534.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51030..51965
                     /gene="lysR"
                     /locus_tag="JNY02_000898"
     CDS             51030..51965
                     /gene="lysR"
                     /locus_tag="JNY02_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBB9432535.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51952..52644)
                     /gene="ygeA"
                     /locus_tag="JNY02_000899"
     CDS             complement(51952..52644)
                     /gene="ygeA"
                     /locus_tag="JNY02_000899"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBB9432536.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52773..54186)
                     /gene="araE"
                     /locus_tag="JNY02_000900"
                     /pseudo
     CDS             complement(52773..54186)
                     /gene="araE"
                     /locus_tag="JNY02_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(54501..55262)
                     /gene="kduD"
                     /locus_tag="JNY02_000901"
     CDS             complement(54501..55262)
                     /gene="kduD"
                     /locus_tag="JNY02_000901"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBB9432537.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55292..56128)
                     /gene="kduI"
                     /locus_tag="JNY02_000902"
     CDS             complement(55292..56128)
                     /gene="kduI"
                     /locus_tag="JNY02_000902"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBB9432538.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(56448..57596)
                     /locus_tag="JNY02_000903"
     CDS             complement(56448..57596)
                     /locus_tag="JNY02_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBB9432539.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            57851..59080
                     /locus_tag="JNY02_000904"
                     /pseudo
     CDS             57851..59080
                     /locus_tag="JNY02_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14657 a        15972 c        14484 g        14332 t
ORIGIN      
        1 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
       61 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgcc
      121 atacaattta tgttgcaacg caaacgtttc cctattttca taaaccgtat tttatcgcta
      181 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
      241 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
      301 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt
      361 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
      421 aaattattac agcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
      481 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
      541 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
      601 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
      661 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
      721 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
      781 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
      841 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
      901 aatcaccaat accgttgaaa gcttcatgtc tcaccagctg agtatgacgc caccgccgcg
      961 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca
     1021 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
     1081 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
     1141 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
     1201 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
     1261 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
     1321 gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc
     1381 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
     1441 ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
     1501 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
     1561 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
     1621 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
     1681 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
     1741 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg ggctgattgc
     1801 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
     1861 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
     1921 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
     1981 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
     2041 cggctcgctc tctctgactg gtaaaggcca ccacacagat atctccatta ttatgggcct
     2101 ggcgggtaac ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt
     2161 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
     2221 tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac
     2281 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
     2341 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
     2401 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
     2461 cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc
     2521 gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
     2581 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
     2641 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
     2701 agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
     2761 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa
     2821 ctcactggct cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc
     2881 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
     2941 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga
     3001 aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt
     3061 accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc
     3121 actgcgccgt accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
     3181 aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa
     3241 gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt
     3301 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
     3361 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt
     3421 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt
     3481 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
     3541 tggcgtcatc agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg
     3601 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
     3661 gccagcggca acgaagccga tgacttagcc gccacactgg cggtcaaagt gacactggcc
     3721 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
     3781 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
     3841 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
     3901 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
     3961 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
     4021 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
     4081 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
     4141 gccgcaaatc gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct
     4201 ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg
     4261 ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg
     4321 gaataccgac atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt
     4381 ccaggctcat accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
     4441 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
     4501 gtggagtgtt ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
     4561 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
     4621 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
     4681 gtgcatcggt tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
     4741 catcgacacc cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa
     4801 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt
     4861 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg
     4921 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
     4981 caaactccgg gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac
     5041 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
     5101 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
     5161 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
     5221 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
     5281 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
     5341 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
     5401 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
     5461 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
     5521 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
     5581 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
     5641 taccgccagc cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa
     5701 ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
     5761 aggctgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
     5821 tgccatcaat aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
     5881 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
     5941 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
     6001 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
     6061 ttagccgttt aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt
     6121 atattttgtg acctagctca actaatcaca gtaaataact gcaagttctc tttttataac
     6181 cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc
     6241 tcgccagaaa tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt
     6301 tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat
     6361 aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg
     6421 aaaatgaatc catgagttca tttcagacag gcaaatattc accgatatga agcccgaact
     6481 cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga
     6541 ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc
     6601 agggcaaagc agaagttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg
     6661 ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct
     6721 gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc
     6781 aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt
     6841 atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac
     6901 cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc
     6961 aaaccctttt gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc
     7021 gcaaacattt aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt
     7081 gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag
     7141 tgcgtataaa cacagcttgg tattatcggt acagacacct tatatgatca tcgtggctat
     7201 cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa
     7261 tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg
     7321 tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg
     7381 gagctatttg attcgctacg ctgtagaaga aattccaggt atgactgcag gctttgccgc
     7441 taactattta accggaacca tggtgtgctt ctttattggt cgtatcaccg gtacctggct
     7501 catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact
     7561 gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag
     7621 cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca
     7681 ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac
     7741 tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccactgctg aactgatccc
     7801 cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa
     7861 ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa
     7921 attggtatcc gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa
     7981 caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatacc
     8041 tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc
     8101 gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct
     8161 tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg
     8221 ggctttaacg gcactgaacg ccccggcgct gtttacctgg cagcggctct ggcagctcac
     8281 agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac
     8341 acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc
     8401 gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc
     8461 ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg
     8521 gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa
     8581 atggcactgg cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag
     8641 tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg
     8701 tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa
     8761 tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa
     8821 tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg
     8881 cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg
     8941 ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca
     9001 gaagcaattg agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc
     9061 catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa
     9121 ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc
     9181 gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc
     9241 cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc
     9301 ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat
     9361 gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc
     9421 actggtaaag ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac
     9481 ggagttctga ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt
     9541 atcccggtat gtatgcacaa cgttgaagag accaaagtgt atcgtccttc tgcctgggct
     9601 gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg
     9661 tacaagcgtt aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg
     9721 aacaagcaag agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt
     9781 atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg
     9841 gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg
     9901 atggaaaaca acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca cgatagccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 ctccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca actaaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggtatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccgggatct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc
    15121 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    15181 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc
    15241 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    15301 tttatacgaa atcataccgg tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    15361 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    15421 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaagcgatt
    15481 aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    15541 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    15601 tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt
    15661 ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg
    15721 acagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca
    15781 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    15841 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta
    15901 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    15961 cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg
    16021 tcccctggct gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg
    16081 cgcagcgact gccggatgtc gatttgttgc cagaactgat tactccccgt agtcggattc
    16141 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    16201 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg gcggtgcatt
    16261 tccccgcgga tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt
    16321 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc
    16381 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat
    16441 ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg
    16501 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    16561 tagcaacgct tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc
    16621 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    16681 tactggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg
    16741 cagaattagg cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg
    16801 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    16861 aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    16921 taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    16981 acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    17041 aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcga
    17101 cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa
    17161 aaccgccgcc gaattacagg cacagtcgcc gctggcattg tttgatgagc tggaattacg
    17221 tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc
    17281 tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga
    17341 cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac
    17401 cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc
    17461 catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg
    17521 gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt
    17581 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat
    17641 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    17701 ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    17761 tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt
    17821 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    17881 gcgctccgcc atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg
    17941 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    18001 cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat
    18061 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    18121 acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg
    18181 aggttatccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc aggcctacat
    18241 aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca
    18301 tccggtatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcaccctg
    18361 tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc
    18421 ccggagccgt tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc
    18481 cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga
    18541 catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca
    18601 gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa
    18661 cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg
    18721 gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt
    18781 gtgttttgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac
    18841 ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga
    18901 aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa
    18961 tgaagttatc catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgccccgg
    19021 cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat
    19081 actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa
    19141 actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc
    19201 gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac
    19261 tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc tcggcaaaat
    19321 caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg
    19381 taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa
    19441 gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct
    19501 tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga
    19561 gcagcctctc cctgacgcaa attttgcaca aaaaataagc ttagtggttt gtttttgttc
    19621 aaaatcatgc taaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag
    19681 tatagtgcgc atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc
    19741 cgaaggtcgt cggttcaaat ccggcccccg caaccaatca aatttgatga agtaaaagca
    19801 gtacggtgac gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt
    19861 cggttcaaat ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
    19921 ctgatctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
    19981 ttgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct
    20041 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
    20101 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
    20161 ttttatgcag cttgttgatt tttccgagct tattcagtac cgctttacca aacttaaggc
    20221 tgtcagcaat agtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
    20281 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
    20341 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac cgaggaaccg ctcggctgac
    20401 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
    20461 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
    20521 tattgccctc tttctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt
    20581 tttcgcgcgt tttgtatttc cccaccgcac cggaatcttc gccaccgtgg ccagggtcaa
    20641 gcataatgac aatcggacga tcgcgccctg ctttacccgg ttgtggacca ctttgcgctg
    20701 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
    20761 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
    20821 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc
    20881 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
    20941 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
    21001 ccacgcgttc agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
    21061 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg
    21121 cagccaggct gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac
    21181 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
    21241 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
    21301 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
    21361 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
    21421 aaataatcat gcaaagaggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
    21481 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
    21541 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
    21601 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
    21661 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
    21721 aacactagaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
    21781 gtcaatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa
    21841 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
    21901 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gaaagcggtg caatagtgct
    21961 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
    22021 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
    22081 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
    22141 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
    22201 tggcgcagtg aaagcctgcc gcagcggcgt gcgccgctgt catttaatca gttatcagga
    22261 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
    22321 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
    22381 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
    22441 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
    22501 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
    22561 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
    22621 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
    22681 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
    22741 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaatca ccggatgcgg
    22801 caggcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
    22861 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
    22921 tcggcataca gcgacagacg acgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
    22981 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
    23041 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
    23101 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
    23161 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
    23221 accggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
    23281 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
    23341 cacaaaagct ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
    23401 agcagatcca gataacgtcc gtaacccgca agagcttcct caagcatcgc aatataatct
    23461 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
    23521 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
    23581 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    23641 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
    23701 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    23761 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatctggcaa ggcgtcgatc
    23821 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    23881 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
    23941 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    24001 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    24061 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc
    24121 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    24181 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    24241 tccagggttt gcgccagtag agcttcatca acctcaatgg tatgattcac ctcattgaag
    24301 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    24361 aggagcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    24421 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
    24481 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    24541 aacagtgccg ccgcgagggt gacagcagga tgttcatctc ccgccacggt cagggcaaat
    24601 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    24661 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
    24721 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
    24781 ggaacagata gataacgccg ccaaagtgaa gatcatagtc gtaatcagca atgcgatggc
    24841 gcagataacg atgcagtgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    24901 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    24961 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    25021 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
    25081 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
    25141 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
    25201 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
    25261 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    25321 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
    25381 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    25441 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
    25501 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
    25561 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
    25621 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    25681 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    25741 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    25801 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg
    25861 cagcattaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    25921 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
    25981 tatggatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
    26041 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    26101 tttccagctg cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac
    26161 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    26221 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    26281 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
    26341 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
    26401 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    26461 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    26521 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgt
    26581 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    26641 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    26701 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    26761 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
    26821 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
    26881 aagtgtagtg ggcgtgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
    26941 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    27001 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    27061 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
    27121 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    27181 tttcacgcgc tactgtttcg cggatctcag ccaatgctcg ggtgatcacc agatcgcgga
    27241 tcgacaatgg ttctgcaagc agttgatcga tcacctcaaa cagtggatgt cgcggggttt
    27301 cccccccggc cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    27361 gcaactgata actgtttgtc tcttcttctg cccaagcgct gatcttttcg atccatttag
    27421 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    27481 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
    27541 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc
    27601 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    27661 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    27721 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    27781 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
    27841 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    27901 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    27961 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
    28021 tgaccaccag cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    28081 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    28141 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    28201 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    28261 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
    28321 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    28381 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    28441 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    28501 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    28561 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    28621 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    28681 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    28741 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    28801 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    28861 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    28921 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
    28981 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    29041 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    29101 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    29161 tggttatacc aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac
    29221 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    29281 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    29341 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    29401 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
    29461 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    29521 ggatggtcgt atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta
    29581 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    29641 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    29701 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    29761 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    29821 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    29881 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    29941 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    30001 gccaggcctt tatcagttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    30061 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
    30121 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
    30181 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
    30241 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    30301 gtttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    30361 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
    30421 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    30481 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcactgacc
    30541 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    30601 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg
    30661 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    30721 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    30781 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    30841 tacgcctcgg gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact
    30901 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    30961 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    31021 gcctgactta atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg
    31081 cggggcattc acggacctca taatcaactt tattttctgg acagattcaa caggttaacg
    31141 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    31201 tcagtccgtg actggtgcgc atcataaagt aagcggatag attgcgcaat ttttatacaa
    31261 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    31321 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    31381 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    31441 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    31501 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
    31561 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    31621 accgtctttg cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag
    31681 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    31741 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    31801 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    31861 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    31921 tgccgcccgg aagcggcgga acaagcgttc agcatcatcc tgctcaacca gtgcattcaa
    31981 taactgctga ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg
    32041 gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    32101 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    32161 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    32221 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    32281 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    32341 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    32401 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    32461 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    32521 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    32581 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    32641 cagagtacaa atattaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    32701 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    32761 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    32821 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    32881 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    32941 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    33001 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    33061 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    33121 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    33181 gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa
    33241 gcgactgtca ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
    33301 cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    33361 tgcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    33421 agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc
    33481 acgctgcggg ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg
    33541 tttgttatcg ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag
    33601 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc
    33661 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    33721 cccccatgag gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    33781 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    33841 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    33901 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    33961 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    34021 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
    34081 ccagagcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggggcttg
    34141 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    34201 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    34261 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    34321 ctccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    34381 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    34441 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    34501 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    34561 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    34621 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    34681 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    34741 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    34801 ctgccaacca tgccgccaga gctgttggta ctggtttctg ctggcgagac tgttcattaa
    34861 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcgcaaccat
    34921 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa tgccccctct
    34981 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatttttcc atcaatgact
    35041 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    35101 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    35161 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    35221 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    35281 attccttgca atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    35341 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    35401 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    35461 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    35521 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    35581 tcagacgaaa tccaatctgg tcggactctt ttaccggttc gcgatcccag ataccgttac
    35641 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    35701 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    35761 gcagccagat ttcatcttcc agtgccagct tacgagtgct cgttaaactt tcacgctgta
    35821 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    35881 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    35941 ataaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    36001 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    36061 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    36121 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    36181 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    36241 ccgcgcctag ctggcggttt gccaaagccg ttgcgactgc tgccagtatt gccagccata
    36301 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    36361 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    36421 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    36481 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaccgacgc acactggcgt
    36541 cggctctggc agtatgtttc gtaattagat agccaccggc gctttaatgc ccggatgtgg
    36601 atcgtagcct tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg
    36661 tttgataatc aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc
    36721 catatggttg ctgtacaggt gcgtatcgcc accggtccag acaaaatcac ccacttccag
    36781 atcgcactgc tgcgccatca tatgcaccag taacgcgtag ctggcaatgt tgaacggcag
    36841 gccgaggaag acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac
    36901 atagaactgg aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt
    36961 ccacgctgaa acaataatgc ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt
    37021 agtaatctgg tcaatatgac gaccatctgg cgttggccag gcgcgccact gtttaccata
    37081 cactggcccg aggtcgccat tttcatcggc ccattcgtcc cagatggtga cattgttttc
    37141 gtgtagataa gcaatgttag tatcgccctg caggaaccac agcagttcgt ggatgatgga
    37201 acgcaggtgg caacgtttgg ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat
    37261 ctgatgacca aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc
    37321 gtcgagcact ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg
    37381 ctgtgggctg cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat
    37441 ggaaagaatt tgccccatgc tgatgtactg cacccaggca ccggtaaact gcgcgtcggg
    37501 ctggcggaaa aactcaacaa tgatgcgaaa cgcgccgtag ccaatcagga acaagcctga
    37561 gacagctccc attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac
    37621 accttccagc agcagttcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc
    37681 gaaaatggat tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc
    37741 tgggaacagc atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat
    37801 aaagttgccc agacgcccgg caccaagacc aaacggaatg agtggtgcga taaaatcaga
    37861 gacctggaag aaggaacgtt tagtacggcg ggcgaagata atcatcacca cgataacgcc
    37921 aatcaggccg ccgtggaaag acatgccgcc gtcccagaca cggaacagat acagcggatc
    37981 ggccataaac tgcgggaaat tgtagaacag aacataacca atacgtcccc cgaggaagac
    38041 gccgaggaag cccgcataga gtaagttttc aacttcattt ttggtccagc cactgcccgg
    38101 acgattcgcc cgacgtgttg ccagccacat tgcaaaaatg aaacccacca gatacatcag
    38161 gccgtaccag tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa
    38221 atgcagatag ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac
    38281 agcgattgaa atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag
    38341 gggacctggc tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc
    38401 tccgcgaatc agcccgccca tgccgcgacg ctccataaag gctgcaacct gatgacgaac
    38461 ttcggtcgcc agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cggcaaaatc
    38521 aatgcgccgc agcaggtatt ttacccgcgc tacagaacgt ccgttcatag acagatggcg
    38581 atacccaagc ccaatgagga ttgccacgca catgggatcg cccgccattt caccgcacaa
    38641 acggagatcg ataccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat
    38701 tgcaggatga agactgtcgt aaatgttcgc cacccgggta ttgttgcgat caacggccag
    38761 aatgtattga gtcagatcgt tggtgccaac agagatgaaa tcgacccgct ttgccagatg
    38821 cggcagcata aataccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat
    38881 ttcgtaaccg atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc
    38941 gtcaacttca tcgaggctgg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc
    39001 attagcacgc agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat
    39061 gcgaatacca cgccaaccca ggcatggatt ctcttcgctg atcggcatgt aaggcagctg
    39121 cttatctgct ccgacatcca gcgtacgcaa ggtgacgggt ttatcattga acatttgcag
    39181 catcccctga tactgcgcca cctgttcttc ttccgacggg aaaccacttt gcagcatgaa
    39241 tgggatttca gtgcgataaa gaccgatgcc atcaatacgg ctgcccagtt tttcttcatg
    39301 ttccgggctt aaaccagcat tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc
    39361 gggtaaattg acgtcatctt ccgccagacg gctaagctca atctcttcac taattagccg
    39421 ctgatattct tgcagcagta ccggctccgg atcgaccagc aattcaccgc gatagccatc
    39481 aacgatcagc gtcctacgat gcagcaccga aggctgaata tccgcgccca tcacggtagg
    39541 gatccccagc gcacgtacca tgatcgcagc atgggagttg gctgcgccat ctcgcacgac
    39601 aacaccgact aagcgatcct ggggcagctc agcaagagtt gtcgctgaca gttcatctgc
    39661 caccagaatg aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa
    39721 cagcaatcgc tgacccagcg cacgtaaatc gccagcccgc tctttgagat agttatcgct
    39781 tagcgcggca aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac
    39841 cgagccttta tcaacctcgg caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg
    39901 cgagtaaaga tcgaaaatag ccgccgtttc tttttgtgct ccggcggcaa agcgtttgct
    39961 gtagcggcga aactcgtttg ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag
    40021 agccggatcc agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg
    40081 ccagccttcg gcaatcgcca caccaggtgc tgccggtaat gcgcggattc gcgtctggcg
    40141 atattgccca aacagggcag tcaactgcga ctgagaaaga atagctgcca tctgggtggc
    40201 aagcgtcacc aggaaggatt cttcactttc gtcatactgg cgcaactctc gttgctgtac
    40261 caccagtaca ccaagcaact ggcgacgttg aataattggt acgcctaaaa acgcgcggaa
    40321 acgttcttct tttatggagg ggatgtattt gaagctgggg tgcttttgcg catctgcaag
    40381 gtttatcggt tccgccagcc tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt
    40441 tacagtgcga ccgcgtggtt ttttcagccc ccgggtcgcc atcaggtagt aacaacgtcg
    40501 atcatgatcg gccaggtaga ccgaacagac ctcggtatcc atcgcaagac agatgtcggt
    40561 aaccagaata tttaacgcct cattcaggcg tggtgcgctg gctacctttt cgactatttc
    40621 gcgcaggcga gtgagcataa ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc
    40681 gttttgtggt tttggcgtat tttcctgcag tgacatcacc acactcgcga actctttcat
    40741 taccctacgg tagacatcac gtttaaatga caccacctgt ctgaccggat accagtaact
    40801 tacccatcgc cagccgtcaa actctggtgt actgctggtt tgcatattga tttctgcatc
    40861 gccgcttacc agctgcaaga gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt
    40921 gtcccaacgc accaaacgtt tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag
    40981 gattcgaacg tctttgcggc ttaatcctac ttcttcaaac aattcacggt acatcgcctg
    41041 ctctgcggat tctccggggt tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa
    41101 tcgccgggcc cacattacct gcccctggcg attacaaatc acgataccta cgtttgggcg
    41161 gtagccatcg tcatcaatca ccggactacc tcaaaataaa gctttatata cgaatgattg
    41221 tttcatactc caggaagacg gtaaaccact ctctgcaggg cattacacac taataacaat
    41281 tgaataactc acagttatgt gcagagttat aaacagagga aggggtggat agccgttttt
    41341 attcaccttt tctgtggata gagttgtgaa gaactacgga attactacgg gaaaacccgg
    41401 agcattctga ataagcccga tatagacatt ttaaaatatt cattattagt caatgcatta
    41461 catgtaattt gagtaatgaa aaagagtaat tcgtgaccca ggtcacacct ctcatttacg
    41521 ggttggcgaa agatcaacca atgccgtatt tatccacaga atgtgccact aagttaagca
    41581 ctgaaccact aaaaactgga gtttcgtcgc acgtcaaggc tgtaaatgaa aacagtagtg
    41641 gaggtttttc acagttatcc cagctttctg tggataacat ggtgtaagat cctgtttatt
    41701 ttcagtgacc agatttggaa aacctatttc agtgttgcgc aactcgatta ccggcagcct
    41761 aaaaagcaat ataaatcaga gaattgaaca acacatgtgg aaaaagttac actgcgaata
    41821 ttcggcacat aattgctgtt tgttttttaa ccaaggcatc atgacatgtc ccaacctcgc
    41881 ccactgctct ctcctcccga aactgaagaa cagttgttag cgcaagcaca gcaactttct
    41941 ggttatacat tgggagaact ggcggcactt gccgggctgg ttacgccaga gaatttaaaa
    42001 cgcgataagg gctggattgg cgtgttactg gagatctggc taggtgccag cgcagggagt
    42061 aaacctgagc aagattttgc tgctctgggc gtggaactta aaactatccc tgtggatagt
    42121 cttggtcgtc cgctggaaac aacattcgtt tgtgttgccc cgttaacggg taatagcggg
    42181 gtgacctggg aaaccagcca tgtgcgccac aagctcaaac gcgtactgtg gataccggtt
    42241 gaaggcgagc gcagcatccc gctggcgcag cgtcgcgtag gatcaccgtt gctgtggagc
    42301 ccgaatgaag aggaagaccg acagctacgc gaagactggg aagaattaat ggatatgatt
    42361 gttctcggtc aggttgagcg gatcaccgct cgtcacgggg aatatttaca gataagaccg
    42421 aaagcagcga atgcgaaagc gcttaccgaa gccattggtg cccggggcga acggattctg
    42481 acgctgccac gcggctttta tttgaagaag aatttcacca gtgcactact ggcccgtcat
    42541 tttctgatcc agtagccatc gctttgacct gccgctttcc gggcatataa ttaccgcttc
    42601 atttttttgg cagggctttt tagatgttat ttgcatggat aaccgatcct aacgcctggc
    42661 ttgcgctcgg tacgctgacg ctgctggaga tcgttcttgg gatcgacaat attattttcc
    42721 tttctctggt ggtggcaaag cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt
    42781 tggcgggagc catggttatg cgtctggcgc tgctggcatc aatctcttgg gttacgcgcc
    42841 tgacaaatcc gctttttaca atattcagtc aggaaatttc tgcccgtgat ttgattctgc
    42901 ttctgggtgg cttgttcctt atctggaaag ccagcaagga aatccacgaa tccattgaag
    42961 gtgaagaaga agggctgaaa acacgcgttt catcattcct cggcgctatc gtgcagatta
    43021 tgctgctgga tattatcttc agcctcgact cggtgattac cgctgtgggt ctgtcagatc
    43081 acctgtttat tatgatggca gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc
    43141 gctcgattgg tgattttgtc gaacgccatc cttcggtaaa aatgctggcg ctctctttcc
    43201 tgattctggt gggctttacc ctgattctgg aaagtttcga catccacgta ccgaaaggtt
    43261 acatctactt cgcgatgttc ttctctattg cggttgaaag cctcaacttg attcgcaaca
    43321 aaaagaatcc gctctgatac tccgtacgct ctcctgcggg agggcgtact cactcctttt
    43381 accctcacag tttaagattt tcctgctttc aagatatata acgtcgattt ataaacagac
    43441 tattatcata ggtaggccaa atgaggataa ccggatgaaa aaatgggcag taataatttc
    43501 tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt gattacgtca tggcgaccaa
    43561 agatggccgt atgattttga ccgatggaaa acctgaaatt gatgatgata ccgggctggt
    43621 gagttatcac gatcagcaag gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat
    43681 tattgaacgt taacaaataa ggtcagcatc cggctggcct taagattttt ctctttccct
    43741 tttcccttcc ctctgccatt tttatattcc ttatgtcgtg attataaaaa ggaaacggct
    43801 atgcaatatc accgtatacc ccacagttcg ctggaagtca gcacgctggg gcttggcacg
    43861 atgacgtttg gtgaacagaa cagcgaagcc gacgcccacg cacaactcga ctatgccgtc
    43921 gctcagggca ttaaccttat cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa
    43981 acgcaagggt taaccgaaac ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa
    44041 aagttaatta tcgcctccaa agtgagcgga ccgtcgcgca ataatgacaa gggcatccgc
    44101 ccggatcagg cgctggatcg gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgc
    44161 ctacagactg attacctcga tctttatcag gtgcactggc cgcagcgccc gaccaactgc
    44221 ttcggcaaac tcggttatag ctggacggat tctgcgcctg cggtttcgct gctggatacg
    44281 ctggacgcac tggcagagta ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac
    44341 gaaactgcat ttggcgtaat gcgctacctg catctggcgg acaaacacga tctgccgcgt
    44401 attgtcacca ttcagaaccc ttacagtctg ttaaaccgca gttttgaagt aggtctggca
    44461 gaagtcagcc agtatgaagg ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg
    44521 ctgaccggga aatatctcaa tggtgcaaaa cccgctggcg cacgtaatac gctctttagt
    44581 cggttcaccc gctatagcgg tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc
    44641 gccagacgtc atggccttga tcctgctcag atggcgctcg cgtttgttcg ccgtcaaccg
    44701 tttgttgcca gcactctgct gggcgcaacc acgatggatc agctgaaaac taacatcgaa
    44761 agtttgcatc tggagttaag cgaagacgta ttagctgaaa ttgaagcggt acatcaggtt
    44821 tatacttatc ctgcaccata aagattaacg ccggttaaaa ccggcgttaa atattaatga
    44881 cggcgctgcc agatccacag cgccgttatt gccagcgcaa atagcgcacc gaagccaatg
    44941 ccaatgggca cgaccgggat gcctaccatt accgccagcg agtaaatgcc cagcatcaac
    45001 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    45061 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccagcacg
    45121 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga gaaaatcagt
    45181 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaacc
    45241 agcttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    45301 gtcggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    45361 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    45421 ccattgcacc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    45481 caggactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgca
    45541 ccaccgtagg ccagtacgca tgcggccagg gcgaccagga catgccagtc agccagcaca
    45601 ccaccggcta cggaaccgag taaaatcgcc gctatggcag aagcttccat taaaccgtta
    45661 gcttttacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    45721 taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat accaaagcag
    45781 atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    45841 aagctatccg ccacctgccc gacaaacggc gccaaaagaa tgtaagcacc tacaaacacc
    45901 atttgcagga tgggctggct ccactccgga cagaactgcg ctttcagtaa cgccagagtg
    45961 gcaaacagta gcgcgttatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    46021 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    46081 ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    46141 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    46201 atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    46261 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    46321 tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt
    46381 tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc
    46441 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc
    46501 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    46561 gccctcttcg ataccaggga ccgacaacag gcgtgcatcc attcctggta gaatacgccc
    46621 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcattcggt
    46681 cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    46741 ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    46801 ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg tgcaactgcg
    46861 gtcatacacc aagtccggca caatgcggta atgcagcggg cttggataaa ggaacacttc
    46921 tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    46981 taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    47041 gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt cagaaccgga
    47101 ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca ggcgcggcat
    47161 cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc
    47221 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    47281 gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaataa cactggtcag
    47341 cccttttacc ccggcagtgt agttcatcat tgcgggaatg cggcgacgcg cgatggcccc
    47401 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    47461 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    47521 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    47581 tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac gcgcttccat
    47641 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc
    47701 cggcatcgcc acctgcgttg gaggcaaaat atgcagagta atttgcggga acaagcgacg
    47761 tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg
    47821 aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag
    47881 cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac
    47941 cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    48001 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac
    48061 tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg
    48121 cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata
    48181 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    48241 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    48301 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc
    48361 gtaccgaata atctcaccaa tcaatacctc tgggatcacc actttagcaa cctgaagcca
    48421 aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc
    48481 tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa acacgccacc
    48541 ccttgaacca acgggcgttt tccgtaacac tgaaacaatg taagcgttta cccactaagg
    48601 tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag tcgccaccgt
    48661 ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggctgg ctgtgcatag
    48721 tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac
    48781 cactgaaacg gtcggtctga tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt
    48841 gaaagcggtc gaacaggtgg cttatcacac cggtaatttc ttattgattg gcaacggtta
    48901 ccacaacgaa caaaaagagc gtcaggctat tgagcaactg atccgccatc gctgtgctgc
    48961 gttggtcgtc catgccaaaa tgatcccgga tgccgattta gcctcattaa tgaaacaaat
    49021 gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt gtattgctct
    49081 tgacgatcgt tacggtgcct ggctggcaac gcgtcattta attcagcaag gtcatacccg
    49141 cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc tgcaagggta
    49201 ttacgatgcc cttgctgaaa gtggtattcc ggccaatgat cggctggtga catttggcga
    49261 accagacgaa aacggcggcg aacaggcaat gaccgagctt ttgggacgag gaagaaattt
    49321 cactgcggta gcctgttata acgattcaat ggcggcgggc gcgatgggcg ttctcaatga
    49381 taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg tgctggtgtc
    49441 acgctatgtg cgtccgcgcc tgaccaccgt gcgttaccca atcgtaacga tggcgaccca
    49501 ggctgccgaa ctggctttgg cactggcgga taatcgccct ctcccggaaa ttactaatgt
    49561 atttagtccg acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg aggcaagtca
    49621 tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat tcttcgatgg
    49681 tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct ggtaacagcg
    49741 gacggctatt gtagttggat gacattgatg cgccatatgc ccctgtatca tgcagtacca
    49801 gataatcacc tgccttcact tccggcaagg cgcgggtttc aacatttccc ccttcctgct
    49861 gggtaaagac atcgcccgat tcacataacg gcccggcgac gacggtttcc accgttggcg
    49921 cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa ctaccgtaca
    49981 ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg cggctaccca
    50041 tttgtttgac gctccgcacc tgagtaatta atacgccaga ctgcgctacc aggaagcgac
    50101 ccggttcaat ttccagtttc acagggtggc ccaaatggcg agcgatttgc tcacgcgcgg
    50161 cattccacag accataataa tgttcggtat caaccgcctc ttcaccctgt tgataaggaa
    50221 tagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg atgacctgac
    50281 gcaccatagc accacacacc tgctccagat gggcataatc aacgccagaa ccaatgtgca
    50341 tgtgaatgcc gactagctgc agatgatgac gttgtatcac gtccagtgcg gcgggcagat
    50401 cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt tggctatgtc
    50461 cgtgaccaaa ccccggatta acgcgcagcc atacccgatg ccctggcgaa acctggccca
    50521 gttggtcgag catatcaaca gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt
    50581 caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcgggg tgcgtttgcg
    50641 gattgtaacc cgccgccaac gcacgctcta tttcgcctaa cgagacggaa tccactttca
    50701 cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc tgtgcaaagc
    50761 gcaccacttc aaactgtttc agcgctgcaa tctgccgacg aataatttgc gcatcgtaga
    50821 cccacaccgg gcagccaaat tcagcgggca aacgcagcag attttcggcg gtgagatcgg
    50881 tatcggtgct gaacagtgaa tgtggcataa caaactccag atgagtgctt ttttatgatt
    50941 acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgaatctatg caaaaatgat
    51001 atggattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt catattgaaa
    51061 tttttcatgc ggtaatgacc gccggaagcc tgactgaggc agcacacctg ctacacacct
    51121 cacagccaac cgtcagccgc gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt
    51181 ttgagcgcgt acgtggacga ttacatccta ccgtgcaagg actgcgtctg tttgaagaag
    51241 tgcaacgatc ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt
    51301 ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct tttttaccgc
    51361 agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc gtgccccagg
    51421 aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga ctcactgaga
    51481 cactccatac gcctgcggga acagaacgta ccgaattact ctctttagat gaagtgtgtg
    51541 tgttacctcc gggtcatccg ctggcggtaa aaaaggtatt aacgccggat gattttcagg
    51601 gtgagaatta catcagcctt tcccgtactg acagctatcg ccagttgctg gatcagctat
    51661 ttactgaaca tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct
    51721 gcgcaatggt acgggcgggg gtaggtgttt cggtggttaa cccgctcacc gcactagatt
    51781 atgcggcaag cggtttagtg gtgcggcggt tcagtattgc ggttccgttc accgtcagcc
    51841 tgatccgccc cctgcaccgc ccgtcatcag cgctggttca ggcgtttagt gggcatttac
    51901 aagcggggtt accgaaactg gtcacttctc ttgacgctat tttgtcgtca gctacgacag
    51961 cataaaagcg acagcatcct cggcatggat cgccgcggta tcaaacacag gcaaaacact
    52021 gcgctcttct ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc cctgtgcgcc
    52081 ctgttctgca aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt cggtaaattt
    52141 ccccagacac agttcttcaa aaataatctg attaattttc gcccgttcat ccgcttcagg
    52201 aataagacaa ttgatggaaa attgttccgt cagccgcccg cgataaaaat cctgttccat
    52261 ggtgtaacgc gtacccagca gcgccacacg agtcattccg gccccggtaa ttgcacgtcc
    52321 ggtggcatcc gcaatgtgta agaaaggcag agtgcaacgt gactcaatgg catccgccac
    52381 tttatgcatc gtattggtac ataacacaat accttctgcg cccgcccgct gtaagccaag
    52441 cgccgcctca gccagaatgt ccccggtttt atcccattcc ccgcgacgct ggcactcttc
    52501 tatttcatga aaatcgacgc tatgtagcag catttgcgca gagtgaagcc caccaagccg
    52561 ctgtttaatg ccttcattta tcagacgata gtaaggaatg gtggattccc agctcattcc
    52621 tcccagcaaa ccaattgttt tcattcctct ctcctgatgt gtgttaccgc aacaaggctg
    52681 aataaggaac gagacaaacg cctcaacggc caggtgccca atctctatta acgaaaaaag
    52741 ggccggatgt acagcacatc cggcccgtga aatcagacgc cgatatttct caacttctcg
    52801 cctgccatca gtttgcgttc gatatgttcc agcgtgacat ttttggtttc cagaatgagc
    52861 cagaaagtaa tgcccacaaa cgcaatgttc aacgcagtgt agagccagaa cgtaccggca
    52921 gcgccaatgc tatcaagcag tgtcaggaag gccgcgccga taatcatatt cgacacccag
    52981 tttgtcgtcg tcgaacaggt aataccgaaa tcgcggcatt tcagcggctg aatttcagag
    53041 cacaggatcc acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc
    53101 atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg
    53161 caatagccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggt
    53221 ttacgacctg ctttatctac cgtaaagacc gcaatgaagg tcgcgaacat aaaggtcagt
    53281 cccacgacca gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag
    53341 atacgcggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac
    53401 aacataccga gaaacacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg
    53461 ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac
    53521 gtatcgcgca gcatacgcaa cacttcttcc gcctcaatat gacgcccctt ttccgccagc
    53581 cagcgcgggc tatttggcag gaagaccacc agaataatca gcagaactgc tggtaaagca
    53641 agaaccccaa cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg
    53701 ccagcacgat gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt
    53761 acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga cagcaatgcc
    53821 cagcaccaca cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg accctataga
    53881 accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca ggcggaacga
    53941 cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac taaccaccca
    54001 ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg ctccggcgat
    54061 tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat
    54121 attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg tattgatagt
    54181 aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg aacaattcac
    54241 acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca tgccgttatg
    54301 tgatggatat tccaatttta aaattaagtt gaattattga gattattatt aaccacctaa
    54361 ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg attatttcat
    54421 aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg cggcagggtc
    54481 ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc aatggtataa
    54541 ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat caggtcactc
    54601 ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg ttgttcatct
    54661 gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
    54721 atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac tccgcttttt
    54781 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
    54841 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag
    54901 aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga
    54961 gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaattca
    55021 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
    55081 cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg
    55141 ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc
    55201 ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc acttaaaatc
    55261 ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt
    55321 ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
    55381 ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
    55441 caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
    55501 tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc
    55561 acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt tccagtacat
    55621 ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg cctaacgtga
    55681 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc
    55741 aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg
    55801 cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga ccatcgacag
    55861 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc
    55921 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa
    55981 tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
    56041 ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt tttgcgtgcg
    56101 caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
    56161 tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg
    56221 ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga ttaaaaaaaa
    56281 tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta
    56341 tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag
    56401 gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta gcccccgccg
    56461 atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa
    56521 accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg
    56581 acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca
    56641 aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg
    56701 caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg
    56761 gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc
    56821 atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa
    56881 ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt
    56941 ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat taccgggacg
    57001 atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt
    57061 gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt ttcggcggtg
    57121 acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag actgtcaacc
    57181 aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg
    57241 ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga
    57301 gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt gattgcagaa
    57361 acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct
    57421 gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt
    57481 tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca
    57541 ccacgaaagc agccgatagg tgtccgtaac gccccgacaa tcacaacgtc tttcatcaca
    57601 acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt
    57661 ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac
    57721 tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg gcgttaatct
    57781 cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt gtagatgata
    57841 cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta
    57901 cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt cggcaggggc
    57961 tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc tacataaagc
    58021 cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa cgggcgcggt
    58081 aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt
    58141 ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc
    58201 aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat
    58261 tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg gatttttggt
    58321 attcccattg attgcctatt tcttatttct ttccatttac cttgtcggta gttggcaacc
    58381 tgatctatta acaacccagg tagagttcaa tcagaatacc cttcaccaga tatggatatc
    58441 gattcccgtg atggttttcg cctttagcca tacgcccatt atttctacgt ttgccataga
    58501 cagacgtgaa aaatatggcg aacacgctat ggataaatgc aaaaaaatta tgaaagtcgc
    58561 ttatctcatc atctgcataa gtgtactgtt ctttgtcttt agctgcctgc tttctattcc
    58621 atcttcgtat attgaagcgg ctaaagaaga aggggtcacc attttatcgg cgctttctat
    58681 gctgccgaac gccccagcat ggttgtcaat ttccgggatt attgtcgcag tagttgcgat
    58741 gtcgaaatca ttcctgggta cgtactttgg cgttattgaa ggtgccacag aggtcgtcaa
    58801 aacaacacta cagcaggttg gtgtaaagaa aagtcgtgca tttaaccacg cactatcaat
    58861 tatgttggta tcgctgatta ccttcattgt ttgttgcatt aacccgaacg cgatttcgat
    58921 gatttacgcg atcagcggcc cgctcattgc catgatactt ttcatcatgc ctacgctgtc
    58981 aacgtatctc atcccggcgc ttaaaccctg gcgttccatc ggaaatctga ttacgctgat
    59041 cgtgggtatc ctgtgcgtat cggtaatgtt ctttagctaa tatccgttgt ggatggttca
    59101 aaattgctac aattttgaac cattccattt ttcataatat tcatttgtta ttattccgcc
    59161 ctatcacttc tctcgtcata tcctagaatt atcagttagt taaacacaca aagagatctg
    59221 aacatcttaa ataagactat ttaagatgca taacttagat tcgcaagata tcttctggtc
    59281 attcaaaaac aatttgcgat atagatattc tcataccatg agatagttat ctggaccacg
    59341 ttcgaccatg caagattaaa tcagtgaaac ataatcatat ttaataacga gaaatgcatt
    59401 tttaaatatc ttaatcagaa ttatagcaaa tacagattaa tcgca
//
LOCUS       DADWTH010000015        58004 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.015, whole genome shotgun sequence.
ACCESSION   DADWTH010000015 DADWTH010000000
VERSION     DADWTH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.015"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(24..278)
                     /locus_tag="JNY02_000905"
     CDS             complement(24..278)
                     /locus_tag="JNY02_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBB9432540.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JNY02_000906"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JNY02_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBB9432541.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JNY02_000907"
     CDS             1228..2694
                     /locus_tag="JNY02_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBB9432542.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="JNY02_000908"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="JNY02_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBB9432543.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JNY02_000909"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JNY02_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBB9432544.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JNY02_000910"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JNY02_000910"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBB9432545.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JNY02_000911"
     CDS             5709..7265
                     /locus_tag="JNY02_000911"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBB9432546.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JNY02_000912"
     CDS             7347..9161
                     /locus_tag="JNY02_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB9432547.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JNY02_000913"
     CDS             9162..10256
                     /locus_tag="JNY02_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBB9432548.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JNY02_000914"
     CDS             10256..11281
                     /locus_tag="JNY02_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9432549.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGSPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JNY02_000915"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JNY02_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBB9432550.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JNY02_000916"
     CDS             complement(12876..13220)
                     /locus_tag="JNY02_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432551.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNY02_000917"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNY02_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBB9432552.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNY02_000918"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNY02_000918"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBB9432553.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JNY02_000919"
     CDS             15616..17376
                     /locus_tag="JNY02_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB9432554.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKDGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JNY02_000920"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JNY02_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBB9432555.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEINVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNY02_000921"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNY02_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBB9432556.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JNY02_000922"
     CDS             19113..19340
                     /locus_tag="JNY02_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBB9432557.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JNY02_000923"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JNY02_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBB9432558.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JNY02_000924"
     tRNA            21195..21271
                     /locus_tag="JNY02_000924"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNY02_000925"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNY02_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBB9432559.1"
                     /translation="MHQSCSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVDKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JNY02_000926"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JNY02_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBB9432560.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="JNY02_000927"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="JNY02_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBB9432561.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDTNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNY02_000928"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNY02_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBB9432562.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRVEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="JNY02_000929"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="JNY02_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBB9432563.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNY02_000930"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNY02_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBB9432564.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNY02_000931"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNY02_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBB9432565.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNY02_000932"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNY02_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBB9432566.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNY02_000933"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNY02_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBB9432567.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNY02_000934"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNY02_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBB9432568.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNY02_000935"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNY02_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBB9432569.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNY02_000936"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNY02_000936"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBB9432570.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNY02_000937"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNY02_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBB9432571.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNY02_000938"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNY02_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBB9432572.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNY02_000939"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNY02_000939"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBB9432573.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNY02_000940"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNY02_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBB9432574.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNY02_000941"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNY02_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBB9432575.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="JNY02_000942"
     CDS             37391..37555
                     /locus_tag="JNY02_000942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432576.1"
                     /translation="MHTPIGVKPAAGSKEWREAWQKRAFAHISNVYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="JNY02_000943"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="JNY02_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBB9432577.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39359)
                     /locus_tag="JNY02_000944"
     CDS             complement(38328..39359)
                     /locus_tag="JNY02_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBB9432578.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFELVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39593..39952)
                     /locus_tag="JNY02_000945"
                     /pseudo
     CDS             complement(39593..39952)
                     /locus_tag="JNY02_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40170..41812)
                     /gene="yojI"
                     /locus_tag="JNY02_000946"
                     /pseudo
     CDS             complement(40170..41812)
                     /gene="yojI"
                     /locus_tag="JNY02_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
     gene            complement(41888..42482)
                     /gene="alkB"
                     /locus_tag="JNY02_000947"
                     /pseudo
     CDS             complement(41888..42482)
                     /gene="alkB"
                     /locus_tag="JNY02_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42482..43546)
                     /gene="ada"
                     /locus_tag="JNY02_000948"
     CDS             complement(42482..43546)
                     /gene="ada"
                     /locus_tag="JNY02_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBB9432579.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALQENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43620..44675)
                     /gene="apbE"
                     /locus_tag="JNY02_000949"
     CDS             complement(43620..44675)
                     /gene="apbE"
                     /locus_tag="JNY02_000949"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBB9432580.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44787..45908)
                     /gene="ompC"
                     /locus_tag="JNY02_000950"
     CDS             complement(44787..45908)
                     /gene="ompC"
                     /locus_tag="JNY02_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBB9432581.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46646..49318
                     /gene="rcsD"
                     /locus_tag="JNY02_000951"
     CDS             46646..49318
                     /gene="rcsD"
                     /locus_tag="JNY02_000951"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBB9432582.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49335..49985
                     /gene="rcsB"
                     /locus_tag="JNY02_000952"
     CDS             49335..49985
                     /gene="rcsB"
                     /locus_tag="JNY02_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBB9432583.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50185..53034)
                     /gene="rcsC"
                     /locus_tag="JNY02_000953"
     CDS             complement(50185..53034)
                     /gene="rcsC"
                     /locus_tag="JNY02_000953"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBB9432584.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53309..54085)
                     /locus_tag="JNY02_000954"
     CDS             complement(53309..54085)
                     /locus_tag="JNY02_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBB9432585.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54090..55739)
                     /locus_tag="JNY02_000955"
     CDS             complement(54090..55739)
                     /locus_tag="JNY02_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBB9432586.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLAFLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55740..58004)
                     /locus_tag="JNY02_000956"
                     /pseudo
     CDS             complement(55740..58004)
                     /locus_tag="JNY02_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14169 a        15654 c        14265 g        13916 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccatc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gctcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcaatg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gccaggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact tggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcgcccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggca tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctag gtatcgatac ctggtggtat gacgttaata
     9121 aagcggcaaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcg tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcagcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaaca
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc
    13861 acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccctttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa agacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgaccccccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgct ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    17701 taaagaaatc aacgttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagaca ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ttggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttatcc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga acaagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata atgaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgacggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcaacaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 gtatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccacacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaggtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcca gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggt
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtacctctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc aaggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgttg gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac ggttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cgggcggata ccgttactgg ctttgcgcca gcgaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgca gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat gtttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgca gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcaactgcg gtttcggcgt actatctgct
    38401 gcttgcggtt tgtcgtattt cagccccagc acttcgctgg tgtactgcaa ctcgcgttct
    38461 gttgccgcta catcaccgtt cagcttgcgt ccataagacg gaacgatcgc tttcaacgta
    38521 gcctgccatt gcgggctgga aacgcgatcg ccaaatactt tttccagcag attcaacata
    38581 atcggcgcgg cggttgacgc ccctggcgat gcccccagca gcgccgcaat ggtcccttgc
    38641 tggtcactga ccacttcggt acccagacgc agtacgccac ctttctcggc atcacgcttg
    38701 ataatctgca cacgctgccc cgcttgccac aaacgccagt cctctttttt cgcttgcgga
    38761 tagtactctt tcaacgcttc aaaacgatcc tcttcactca acatcacctg actcaccaga
    38821 tatttcacca gctcgaaatt atccagcccg acgtgcatca tcggcatcac gttagaggtg
    38881 gtggtggaac tcatcagatc ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg
    38941 gcaaatggcc caaacagcac tacgcgttta ccgtccagaa cacgggtatc gatatgcgga
    39001 accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa ctttcgccag atggcgatta
    39061 accacgtctg gattttccga aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct
    39121 ttcgcttccg gaatccccga ttcctgtaac agcttcagcg ccgcaccgcc cgcgccgata
    39181 aagacaaatt tggtgcggat gttctgtgca gtgccgtttt tcagatcggc aacggtaacg
    39241 gtccaggtgt tatcgtcatt acgctttagg gcgcggactt cgctgctgag ttgcagcgag
    39301 aagttagatt tcttctgtaa ggaagcaatt aactggcggg tgatctcacc atagttcaca
    39361 tcagtgccaa tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
    39421 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
    39481 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
    39541 cgatattgat aaatgaacgc ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga
    39601 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatgctgc
    39661 catcggcgtt ttgcggggtg tagttcagtt ccatcagtgc agaatgcccg gttccggcgt
    39721 tgttccagcc gttcgaactc tcctgcgcgc ccccctccag gcgctccacc atggtcatcg
    39781 accattcagg ctccagctcg cgtaaatagg tccccaacgt ggcgctcata atgccgccgc
    39841 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
    39901 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
    39961 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacactt
    40021 aagtaacaac atttaaataa tgtttataaa ttatattcta attttagaaa cattataatt
    40081 tcgttgatta attatagggt tattagaaat gaagggattt ttagcatatt gacctgacgg
    40141 cagcagtccg ccaggtcagc gtcgtaatat tatgccgtcc gggcaacggc atcacgcgaa
    40201 gcggcatcgc gctcttcgcc cgtcagctcg ctaagttgcc cattgcgcat ttccagcagg
    40261 cggtcggcgt ggataaagta atgatcatca tgactgatag cgaaaatagt tttacccatc
    40321 tcctgcatca gcggcagcaa cacctgataa aactcacgac ggaagtgtgg atcctgatcc
    40381 gccgcccatt catccagcag gataatatcg cgttcttctg ccagcgccag caacagcggc
    40441 acgcgttttt tctgcccttt tgataacttc aggttaacaa tacgcccgtt gcttaactca
    40501 agcttatgag ccattttcag ctgcgccagc cacttctcaa ccagttgcgg gttagcgggt
    40561 tgcccctccg gccccagcag ttgatcaaac agccagacat cggtaaacac tgccgaaaac
    40621 agtttgcgat aatcttccgg ttgttcgccg ctgacaggtt tgccatccag caagatttcg
    40681 ccgctttgtg gctgatacaa gcccgtcaac aacatcgcca gcgtcgattt tccgctaccg
    40741 ttgccgccaa tcagaaacag cagctcgcca cgtttgatgg tgagattaat cggaccaacg
    40801 gaaaacgcgt tatcctgata agcaaacgtc acgttacgca gctccagcgt ttgccagttg
    40861 ggaaacgcat gcgggcgcgg aaactcagct ttgaaaggcg cgagcgcgaa tttgttcagc
    40921 ttgttaaacg ccacctgcgc cgtcagcagc gtcggcaatg cgccaaccgc cgaaagcagc
    40981 ggcgtacgca ggaataacag cgtcaacgaa taggtcgcag caacgttggt atcagcccaa
    41041 ccgagactgt tcgccatcca gaacaccagg ccgatagcgc ccagcatcat gatgtttgac
    41101 cagttcacgg cactaagatg gaaggtgtct gcgcgaataa tatgatggcg atactcttgc
    41161 gcatcgggga tgtagaggtt gttaaacaca tactcggcgc gttcccggtc agagtcagct
    41221 ctttgcgccc ttcaagtaca gtttgaaaat ccgtgtacag cttgtcttcg gtttcacgta
    41281 gggtcgccat atgtttgtac acccgcgcca ccagcacaaa accgccccag atggtgatcg
    41341 ccatccagat agccgttacc agcaacattt tgcccgacag catccacaga tacgccgatg
    41401 aaccgatagt gagaatgatc ccctgcccca gttccggcag acgcacaaaa gcaatggtga
    41461 tattgcgcac atcgctggtt aatcccgcca gcaacgaggc gctaccgagt tgttcaatgc
    41521 gctcgacgtg ggtatccaga atccgcttga taaattcact acgcagtcgg taaacgaagt
    41581 gatgccccaa agtggtgagc gccagttgcg atccgagagt gactgccatc aacagcagca
    41641 ataatcccag aaactccggt aacaccagca gactggtatc cgccgtttcg ataaggcgct
    41701 gattgataaa agcaattaag ccaatgccta atgccgcact ggcgaggctt agcgccatca
    41761 cactgataaa tggccagcga tactgccgcc agacaagtac aagaagttcc atgcagaaaa
    41821 cccggacaat gaattacagc ccgcagttta aacatcttcg cgcgcacagc aataataatt
    41881 cttattttta ttctttttta cctgcctgac ggaatgtcag gttgtagcgg cagtcggtgg
    41941 tgagtggatg aaaccccgct ttcaacggtt gaataccgtg ataaaacagc cgcgattcac
    42001 cgccccaggc caccaaattg aaaaatcgcg ggtaagccca gagaaacaga aacaattggc
    42061 gcgcgcagat ccggttcgtc tttatcctga tgcagcgaca gtttcgcgcc aggagcgtag
    42121 cggttgataa gacaagcatc tggctggaaa tctggatagc ccgccgccgt agccgcacgt
    42181 tgacataaat catgaaagct ttgtggcatg gcgggccacg gtttatttgt ttgcggatca
    42241 atgggcgaat agagataacc ttgccgatgg gtcgtccagc ccagatgccc acagttggtc
    42301 atcgccaccg acatggtata tccgcctgga gcgaccatct ggcgaaacgg cgactggctg
    42361 gcaacgtcat taatatcgcg gattagttgc tccgcagcat taaaagcaaa acgccgtaaa
    42421 attaccgcac cagccgccag tggctcttgc cacggttcag catcggcaaa cagatccaac
    42481 attacctctc ctcattttca gcttcgcggc gcagcagttg cgctttacgc gacacgcccc
    42541 agcggtaacc ggaaagtgtg ccatcaccac ggaccactcg atgacagggt attacgatag
    42601 ccagcttgtt ggcggcacag gcgctggcaa cggcccgtac cgctttcggt ttgccgatgg
    42661 cgttagccag ttgctgataa ctgacggttt caccacaagg tatcgtgcgc agtgcctgcc
    42721 agacttgttg ctgaaaagca gtgccgcgaa tatccagcgg taacgtcagc ggcgtatcgc
    42781 gttgattgag gctggcgatc acttcacgca catgttgctg aaacgtcaga tcggcaggcg
    42841 cgttgtcggc agcgggaaac atctgctgca actcgctgat taatgtcgcg tcatcatcgc
    42901 ccagcaatat cgcgcaaatc ccccgctcgc tttctgccac caggcaacga cccagctcac
    42961 aatcagccag cgcgtaacgc accgccagat tttcgccacc gtgacggaat tgtttagccg
    43021 tcatgcccag cgtttcatcg gctttgcggt agtaactgct gctgtcgggg aatccggcgt
    43081 taagaataga cgtcgtcacg ctctcccctt tcgccagcga ttcgcgcaaa cggcgagcgc
    43141 gccaggcctg ttgccaggct ttaggcgtca ttccggtagt agctttaaac aaccgatgta
    43201 gatgaaatgg actcatcgcc acctggtcgg ctaaggcttc cagcgttaca ggcgtttcct
    43261 gttccagcag tcgacacgcg tgggtgattt tatccaaccg atgttgccgg ggattggctt
    43321 tgtctggctg acaacgtttg caggggcgaa atccggcggc gagtgcctcg ctggcatttg
    43381 cgtagaagga gacattttcc tgcaaggcat gtctggcgcg gcaagacgga cggcaaaaga
    43441 tacccgtggt gcgcacggcg aaaacgaatt cgccgtcggc attcgggtcg cgggctaaga
    43501 cagattgcca gcgttgatcg tcagttaagc atgtggcgtt tttcataatc agctccctgg
    43561 ttaaggatag cctttagtct gcccggtcac catcacgcaa aaaccaacaa tcttgcgctt
    43621 taattttttt cgctgacaag gaagctttta aactgtggtg acatccaggt tttaaagcta
    43681 tcgccttctt tggtgatcat ataaaccgcc agcccttccc ggcgaacaac ctctttggct
    43741 ttctccggcc cgagtaccat caagccagta tcccaggcat cggcttccag cgccgtcgga
    43801 gcaatcaccg tcacggatac cagattgtgt tcgatgggac gcccggtttg cggatcgata
    43861 acatgggaaa gacgtttgcc gtccagttcg taatagttac ggtagctgcc agaggtgcta
    43921 atcccatgac cgttgatatc caccacagcc tgaaccgcgt tttctttatc ggttggtttt
    43981 tgaatcgcta cccgccacgg ctggccttca ccgttcatac cacggctgtt cagcgcgccg
    44041 cccaccgaca ccagatagcg ggaaatccct tcctgctcca tcaagcgtgc caggtgatcc
    44101 gccgcataac cttcaccgac ggtggagaga tcgacatata aatccggcag gtctttttgc
    44161 agatattgct gatgcgactg attgatgacc gtcaggtgct gtaagccggt tttggctttc
    44221 atcgcatcga tctgttcctg gctcggaatt tgaaccggct gttgttccgg gccaaagccc
    44281 cacagattca ccagcggccc gacggtaata tccatcgcac catcggtcct tgccccaatg
    44341 cgcagcgagg tggtgacgat atcggccatc gcttcactta ccggccacgg cgacaaactt
    44401 tgcgagtcgt taaagcgcat cagcgcggaa tcttttttat aggtcgaaag cagctgatcg
    44461 tcggcgtcca gctgggtctg aatcttttct ttaagttcgg cactgcgttt ggcatcaatg
    44521 cccgggatgc tggcacgcca gaaggtaccc atggttttgc cttcaagaac ggtaacttca
    44581 gtagcgtggg ttttggcggg ttgtggtttt tgatcgcaac caacaaagaa gagcgcggca
    44641 accagcagtg ccacgcgggt aaagcttatt tccatacgtg attatcctca tgcgaacggt
    44701 cgcaagagta caccaaaaaa ctgtgtttgt acgctgaaaa caatgaaaaa agggcccgca
    44761 ggccctttgt tcgatatcca tcgagattag aactggtaaa ccagacccag agctacgatg
    44821 ttatcagtgt tgatgccagc gtcacgagtg aactggttgt cgtccagcag gttgattttg
    44881 tagtcaacgt aggtggacat gtttttgttg aagtagtagg tcgcaccaac atcaacatat
    44941 ttcaggatat cttcgtcgtc gtagttacga ccattgatga cacccaggtt tttaccttta
    45001 gactgcaggt aagctaggga cggacgcaga ccgaagtcga actggtactg agcaacagct
    45061 tcgaagttct gtgctttgtt cgcccaaccc aagttgccta cgcgagttgc gttgtaggtc
    45121 tgggtgtact gagcagctag gtagatgttg ttcgcgtcgt atttcagacc accggtgtag
    45181 gtttcagcgc ggtcgccatt accgatgtaa cgctcatcat aacggttcag accgaagttc
    45241 tggtcatcag tacgtttgga gctggaaact gcagcaccga taccgaagcc ttcgtaatca
    45301 taagtaatag aaccgccaac gccgtcaccg ttctgacgca gagcttcacg accgttgttg
    45361 gtcatgcctt caccttccgg gctaccgttt ttgccctgat actgaacagc gaagttcaga
    45421 ccgtcaacca gaccaaagaa gtcggtgcta cggtaggttg cgaagccgtt accacgctgc
    45481 tgcatgaagt tgtcagaacc gtaagtgtca ccaccgaatt ctggcagtac gtcggtccag
    45541 gaagttacgt cgtaaactac gccgtagtta cgaccgtagt cgaaagaacc tacatcctgg
    45601 aatttcagac ctgcgaatgc cacacgggtc caggagttgt tttcgttttc agcgctgttg
    45661 ccctggatct gatattccca ctggccgtaa ccggtcagct ggtcagtaac ctgagtttca
    45721 cctttgaagc caagacgcat gtaggtctgg tcgccatcta cagacttgtc gtcagagaaa
    45781 tagtgcaggc cgtctacttt accgtacaga tctaatttgt tgccgtcttt gttgtaaact
    45841 tcagcagcgt ttgctgctcc tgctaccagc agagctggga ccaggaggga cagtacttta
    45901 actttcatgt tattaaccct ctgttatatg cctttatttg cttttttatg ccactgcata
    45961 ctgattaacc ctcattaatc agtcggcaag tccattctcc ccaaaaatgc agaataatcc
    46021 aacacgaata tgatactaaa acttttaaga tgtttcattt atcgctatag atgtttcaaa
    46081 atgtaaatac aagggaactt tttaagatta ttgcggaatg gcgaaataag cacctaacat
    46141 caagcaataa taattcaagg ttaaaatcaa taacttattc ttaagtattt gacagcactg
    46201 aatgtcaaaa caaaaccttc actcgcaact agaataactc ccgctatcat cattaacttt
    46261 atttattacc gtcattcatt tctgaatgtc tgtttacccc tatttcaacc ggatgcctcg
    46321 cattcggttt ttttaccctt ctttacacac ttttcattat tctgtgctac cacagaaaaa
    46381 ctataacgct tgttaactat ttcacaaata attaacatca gcataatttc cagcaatctt
    46441 tgtttattta caattaattt cgttgggctt tttgtaggtt atttgtacag caaaatggcg
    46501 cttgtacatc tatttccccc catacaggat gataaatatc acgggagaat agagaatcat
    46561 caatcaggta agagtctgga atttcacact gtacccttta tactgcccta tcacttcgcg
    46621 aagttttaac aggtcataaa cacgaatgcg tcagaaagag acaacggcca cgacccgctt
    46681 ttcactccta ccggggagca ttacccgctt ctttttactg ttgatcattg tgttactggt
    46741 gacgatgggg gtaatggtac aaagtgccgt taacgcctgg ctgaaagata aaagttacca
    46801 gattgtcgac attacccacg caatccaaaa gcgcgtcgat acctggcgtt acgtgaccag
    46861 gcagatctac gacaacattg ccgcgacgac ctccccctcc tccggcgaag gtttacaaga
    46921 gacgcgcctg aaacaggatg tctactatct ggagaaacca cgccgcaaaa cggaagcgtt
    46981 aatctttggc tctcacgaca actcaacgct tgagatgact cagcggatgt ccacttatct
    47041 ggatacattg tggggtgcag aaaatgtacc gtggtcgatg tattacctga atggtcagga
    47101 taacagtctg gtgctgatct caaccctacc ccttaaagat ctcacctccg gatttaaaga
    47161 atcgaccgtc agtgacattg ttgattcacg tcgtgcagaa atgttgcaac aggccaacgc
    47221 cctcgatgaa cgcgaaagtt tttctaacat gcgccgcctg gcctggcaga acggtcatta
    47281 ctttaccttg cgtaccacgt tcaaccagcc gggacatctg gcaacggtcg tggcttttga
    47341 tctgccgatt aatgatttga tcccaccggg tatgccgctg gacagtttcc gccttgagcc
    47401 agacgcgacg gcaacgggaa acaatgataa tgagaaagaa gggacggata gcgtcagtat
    47461 ccactttaac agtacgaaga ttgaaatctc ctcggcactc aactctaccg atatgcgcct
    47521 ggtctggcag gttccttatg gcaccttatt gctggatacg ttgcaaaaca ttctgctgcc
    47581 actgctgctg aacatcggtt tgctggcgct ggcgttattt ggctatacca cattccgcca
    47641 tttctccagc cgcagtacag aaagcgtccc cagcacggcg gtcaataacg aattgcgcat
    47701 tttacgggca attaatgaag agatagtctc actgttgccg ctcggcctgc tggttcacga
    47761 tcaggaatcg aaccgcactg tcataagtaa caaaattgcc gatcatttgc tgccgcatct
    47821 gaatctgcaa aacatcacca ccatggcgga acagcatcag gggattattc aggcgacgat
    47881 caataacgag ctgtatgaga tccgcatgtt ccgcagccag gtcgcgccgc gcacacaaat
    47941 tttcattatt cgcgatcagg atcgcgaagt gctggtaaac aagaaactca agcaggcgca
    48001 gcgtctgtat gagaaaaacc agcaggggcg gatgaccttt atgaaaaaca ttggcgatgc
    48061 gctgaaagaa cccgcacagt ccctggcgga gagtgcggct aaactcaacg ccccggaaag
    48121 caaacaactg gcgaatcagg cagatgtgct ggtgcggctg gtcgaagaaa tacagttagc
    48181 gaacatgctt gcggatgata gctggaaaag tgagacggtg ctgttctccg tgcaggattt
    48241 aattgatgaa gttgtgcctt cagtgttgcc tgccatcaag cgtaaaggtc tgcaactgct
    48301 gattaacaat catctgaaag cacacgatat gcgccgcggc gatcgcgatg ccttacgacg
    48361 tattttgctg ctactgatgc aatatgccgt gacctcaacg caattgggaa aaatcaccct
    48421 tgaggttgat caggatgagt cctctgaaga ccgcctgaca ttccgcattc tggacacggg
    48481 agaaggcgta agtattcatg aaatggataa tttgcacttc ccgtttatca accagaccca
    48541 aaacgatcgt tatggaaagg cggacccgct ggcattctgg ctgagcgatc aactggcacg
    48601 taaactgggc ggtcatttaa acatcaaaac gcgggatggg cttggtacac gctactctgt
    48661 gcatatcaaa atgctcgccg ctgacccgga agttgaagag gaagaagagc gtttactgga
    48721 tgatgtctgc gtaatggtgg atgttacttc ggcagaaatt cggaatattg tcactcgcct
    48781 gttagaaaat tggggtgcaa cctgtatcac acccgatgaa agattaatta gtcaagatta
    48841 tgatatcttt ttaacggata atccgtctaa tcttactgcc tctggcttgc ttttaagcga
    48901 tgatgagtct ggcgtacgag aaattgggcc tggtcaattg tgcgtcaact tcaatatgag
    48961 caacgctatg caggaagcgg tcttacaatt aattgaagtg caactggcgc aggaagaggt
    49021 gacagaatcg cctctgggcg gagatgaaaa tgcgcaactc catgccagcg gctattatgc
    49081 gctctttgta gacacagtac cggatgatgt taagaggctg tatactgaag cagcaaccag
    49141 tgactttgct gcgttagccc aaacggctca tcgtcttaaa ggcgtatttg ccatgctaaa
    49201 tctggtaccc ggcaagcagt tatgtgaaac gctggaacat ctgattcgtg agaaggatgt
    49261 tccaagaata gaaaaataca tcagcgacat tgacagttat gtcaagagct tgctgtagca
    49321 aggtagccta ttacatgaac aatatgaacg taattattgc cgatgaccat ccgatagtct
    49381 tgttcggtat tcgcaaatca cttgagcaaa ttgagtgggt gaatgttgtc ggcgaatttg
    49441 aagactctac agcactgatc aacaacctgc cgaaactgga tgcgcatgtg ttgattaccg
    49501 atctctccat gcctggcgat aagtacggcg atggcattac cttaatcaag tacatcaagc
    49561 gccatttccc aagcctgtcg atcattgttc tgactatgaa caacaacccg gcgattctta
    49621 gtgcggtatt ggatctggat atcgaaggga tcgtgctgaa acaaggtgca ccgaccgatc
    49681 tgccgaaagc tctcgccgcg ctgcagaaag ggaagaaatt taccccggaa agcgtttctc
    49741 gcctgttgga aaaaatcagt gctggtggtt acggtgacaa gcgtctctcg ccaaaagaga
    49801 gtgaagttct gcgcctgttt gcggaaggcc tcctagtgac cgagatcgct aaaaagctga
    49861 accgcagtat taaaaccatc agtagccaga agaaatctgc gatgatgaag ctgggcgtcg
    49921 agaacgatat cgccctgctg aattatctct cttctgtgac cttaagtccg gcagataaag
    49981 actaatcatc tgtaggccag ataagacgcg ttagtgtctt atctggcatt tgcaccgatt
    50041 gccggatgcg gcgtaaacgc cttatccggc ctacgattcc cattatttca acagattaca
    50101 ttaaagtagg ccagataaga cgcgtcagtg tcttatctgg catttgcact gaatgccgga
    50161 tgcggcgtaa acgccttatc cgtcctacga atcccgcgat ttcctgaccc tctcggcata
    50221 taccgtcagc gtctgtttta tcacatccag cgttaccggc ttcgagaggc agctgtccat
    50281 accggactcc agacaccgct gcttctcttc agccaacgca ttagcagtta ctccgattac
    50341 cggcaacgtc agtcccaact gacgaatgcg ttgcgtcaag cggtaaccat ccatatttgg
    50401 catgttgacg tcgctaagca cgatatcaat atgattctta ctaagtacat taagcgcatc
    50461 gacgccatca ttcgcggttt tacattgata gcccaacgat cccaactgat ctgccagcag
    50521 acgtcggtta atcggatgat catccacgac cagaatcatc atatcgtcgt tatcgctgac
    50581 cgctttgtcc gtcgacggca gagcgttagc aggatcgtcg ctctccatct cgatcaaata
    50641 aatacgcgcc aacaatgccg gtagctcatg cggagcagcc acactgtgta cccactcccc
    50701 tggcgctttc tccagcggaa taccaatatg gcgacgacag aaggtcacta ccgctctgcc
    50761 ctgccatttt ttactcacta cctcgtcagt gattaacaca tcttcgggag tcggttcctg
    50821 cccttcgtat gttgtaacga cgatgccgct gcgctgcaaa ctggtttcca ggaactgaca
    50881 gagcgacgca ttgcggaccg ccagccagca gcgtttacca ctcaaccctt ccacgccttt
    50941 tttctgcggg tactgagcgc cgtacaacgg aatacgcacg gtaaactggc tgcccattcc
    51001 cggttctgaa tctaccgaga tatcgccgtc catcatgctg atcagttttt cacaaatcgc
    51061 cagacccaga ccggtcccct ggaagtttcg ctgtacgccc gttccaacct ggaagaaggg
    51121 atcaaacaag cgcaccactt ctttcgccgg aatccccacg ccggtatcgc gaacacggat
    51181 agagagataa tcgccatcag cgcgaacatg caaaactata cagccggtat cggtgaattt
    51241 tatggcgtta ctcaacaggt tggagatgac ctgctgtaaa cgcatcgggt cgccatttaa
    51301 ggccactggc acatccggtt caataaagca gtacaagcct aactgcttgc gtaccaccag
    51361 cggtaaatag ttggcggtga tgtggttcat cacttcacgc ggtgaaaact cacgcggttc
    51421 gatcttcaac tgttccgatt caatcttcga gaaatcgaga atatcgctga taattttcaa
    51481 caacaggctg gaagagttgt tcattgccgt caccagacga tcgacgcctt tcggtaactc
    51541 tttggtttgc aacagatcca ggttaccgat aatgccatac agcggcgttc gcagctcatg
    51601 actgacggtg gcaaggaaca tcgattttga ctggctcgcc tgttccgctg cttgtgccat
    51661 ctcctgcaac gactcttcca tcttcacgcg cgaagaaaca tccaccagca cacaaatggc
    51721 cacgttttca ttacgatagc gcgaatggac gaagctgatt tgcagattgg tattgttgct
    51781 ggtcaggaca tcaacaaaat tgacctgctg cccacagata atttgcgtca gtcgttggcg
    51841 gtcctcatgc gtaagcatat tgagataggt atgcgccagt tcgttactta aaatattgac
    51901 gccatcagcg gtacgcaaaa tgcagatacc cactggcgcg gaggcgacaa tcttgcgatt
    51961 gaactgctca tgttcttcca gtcgcagggc gtcgctttcc gccggaatga aaatacgtcg
    52021 ctcgtacatc cgcgcgagag taaacaatgc agctccggca agcacattca gcaaaattgc
    52081 gttaaggatc aacatgcgaa tgcgttccag caccttatca accggcaccg aatacacgat
    52141 gcttagcgat gagggtggca gatttttctt cagcaccagc tcccggaacc cttccgtata
    52201 gccaaaccag gagcgttcct gcatccagcg aggatcgccc ttaattttac tttctggtcc
    52261 ggtaagcgaa atcagggtat gaccattttc atcaagaatg gtaaccccca tcggcaacgt
    52321 acccggtaag aaaaagttct ccatccggat ggtctgctcg acacccaaaa gcgcctgcaa
    52381 ccggttcgcc agataaactg gcgtcaacgc gtaaaaatac ccgacgccag ggcgcggacc
    52441 ttcgctgatc cagtagaggt tactgccgct atcatcttgt ggtgcatttc gatatttatt
    52501 gatgcgttca tgcaacgctt tcaacgcggt atcgcgttcc actggcatat cacgcagacc
    52561 gaaattggcc atgcagaggt tatcactgcc gattaaaaat acccggttga gatcgtaagc
    52621 cgcagaaaaa ttatcgcgcc agtagcgcat aaaccacgcc aatgactcca gagaacctcg
    52681 ccaggtgtta ctcattgcgg aacaatcgga gtcggcaaac agcggttcaa acgcaggcac
    52741 atccgcctgc gtttctcgtc cacgcgggga aagcacaccg ttttctgccg ataagcgatt
    52801 ttcggcgatg tacttaagct ctttcatcac atcagaggtg cgttgaataa agcgctgagc
    52861 ctgatcggaa ctcagattaa attcctgacg aatttccgat tctcgctgat gtaacgcatt
    52921 aacgatgtaa aaaacggatg aaaaagcaat caacagccag agcactaacg ccaatgctct
    52981 gaacatgtag cgcgaggctt tcagggttgt acgaaaagaa gcaaggtatt tcaaaggggc
    53041 gaagctccgc ctcaggtgac cgatggagtg tggttaaggt agcggtaaaa gcgtgttacc
    53101 gcaatgttct gtcaccattg caataatgcg tatcgcttag aaaccattgc cttaaccagg
    53161 aaagccggag tagatggaca gttctcagta cgaaaaataa gaaatgacca atctgccgac
    53221 gcttgttaat ggttatatcc tggcgtcggc agatcgccca gcagtcttat ctgaaaaaag
    53281 agagagccag gttgtgtgaa gggatggatc accagtcaaa acttttcacc agcgtcagct
    53341 cgccagcatt acgcatcggt acaataaatg tttcctgttt ctcattgacc gatccttcat
    53401 cggtgatcag cgtcagttgg gcggtcgtca attccgtttc gctgcgccca ccatagtagt
    53461 tgatatacac ctgatagcgc ccgtgaactg gtgctggcat ggcgaaaatc tcgggtccgt
    53521 accccgtcgt gacatccata tccagtgcac cactgttttt cagcacggtg ttaccgtacc
    53581 aggcgtgttc gccatcgggc gtgacaacat ggagatcgag atcggtattg tccgtatccc
    53641 acgagagaac cagccgtaaa cgtgcacgaa tagttcctgt acccggcgtt gagtaaaact
    53701 gcattttttg ccggctttgc ccatccgggc tgatgacctg cacgctgttg ctgccttcag
    53761 tgaaaatata agggcgtgca aatgaaccat cggattcaat tctctgcggc atactggcac
    53821 cgttgaccac cagccggccc tgctgaactt tacccgccgc agcataattc tttattttgc
    53881 cgctgatctg cgctgagata ttttgatcat ctgccatgtt gacggacgac gccgggtaat
    53941 tgatcgattg tgaaaaactg gcatcttcgc cctctgccgg atgccagcca gagagcggtg
    54001 cgtcaatctc gacttcctgc acaccttcac tgtgagcgac aggcgaaagt gccaccagta
    54061 acaacggaag aaaaatcttt cgcatagcat tagtccatta ataattgtcg ggtcagcgtt
    54121 tcgatatagt tctcatcaag cccggtggga taaccatcaa aggccaagtg cagatactca
    54181 tgggttaaat ccagccgatc ctgaagcgta aagaagttac gaataaacag ccgtttctgc
    54241 tgccgatcgg tataggggaa acctgagact aaacgacaga ccgcaaacac gtctggttcg
    54301 ttgtaccccg tctcgccttg taatatacgc cgccactgcg gcattttttt cgccagccag
    54361 gctttcgctt tgggtaataa ctggcaggtt gagcgtggag ctccccagcg gctaaggctg
    54421 ttgtcgggat aagcaaacgc gagtatctgg tcgtaacgct ccccctgccc cgcctgggcc
    54481 gtggcttgcc gccaggaaag tgtcccttcg gtagcgcgac tgccatgata atgaaccgga
    54541 tcacctgcat agatgagctc ctgcgtccag gcggtcatcg ttcgcgcccc cgtggtcgct
    54601 ggcgaagcag aaacacgctg cgtggcgctg ctgccgggga tggtcaggca atcaccttcc
    54661 cgattggcgt tttgctgtaa aaaagtacgt atcgccacag tcatggcttt cgctgcctca
    54721 ggtggcgtac ttttcgcctc gcgatccaga actctggcaa cgtactcttc acgatcgaga
    54781 tgcgactgca atttcagttt gcccttttcg ctcagtaagg tcgtctcacc gtggctgaca
    54841 aacgtaatgt ggttaccatt agtaaacgtc acacgatagc gcccattaag tacgctgggt
    54901 tttactgccg tcgtactctt ttccgctgtg attttcttca gcggatagcg ggcaaacagc
    54961 tccacctcta cacattgccc actggcaacc tgcgtcggca ctggcaaaac ccgattaagc
    55021 accgtcgcat aacgggttag taccgttttg ctggtgccac tgccagtaac ccacagcggt
    55081 gtaccatcgg ttaaccagcc agcaaaacca ccctgacgga tttcctgttt atcatcggca
    55141 aaccagctcc aggttttcac ccgtacccga ctaccgagca tggatgagac accgattttc
    55201 gcctcgtcca gcaccacatc cagtaagact tcctgagctt tgttttgcgc aggtaacgtc
    55261 gctaatgaat caagcagcga tttaaccgtt accgaggttt ctggcttcag cgttgtaaga
    55321 aacgctaacc acgcaggtgc ctgacgctgt tgccagtatt gcccccacac atctgcgcca
    55381 atatgcaacc gctgcggcgc aaaatagagc ccacaggaac gtaccagcgc ggtatcgcgc
    55441 gttatgctct ccccagcctg acaacaataa acctcttccg gggagtttcc gcgacactgg
    55501 tagggctgct ccggttgatg ggtatcctcc agccaggcgt aaacatacag tttccacaaa
    55561 ctccctaacg gcgtggtgag cgagtcaggt aatgctgaga ctttggtcac gcctgatgac
    55621 gacagctgat acagttgatc gtgctgcgca ccgcgcagca ccagttgtaa cggcgtctcc
    55681 tccgcaagcg ttggcagagt aaccaacgcc aatagccaga cgattctgcg ccagttcact
    55741 tatttcacct gcatcccggt ccattcactc cctgccgcga cactttgctg cgcaggtgca
    55801 taggaacgca cataacgagc aggaggcaga acgaattgcc ctttttgcga gaagcgcagc
    55861 aaatggcgga aagtgaccgt tcccgtcagt tctttcaccg gcaccatata ggccagttcg
    55921 cccatttcat tacgcgcttt ttccagcaat tgcccctgct gtttcgcggc gttgggttta
    55981 ttgactgaaa tgccccatgt tgtgcgctca acgtcggctc ccggcgggag cggtacttcc
    56041 acctgaccat agcgcagaac tgcatcctgc tcgcaggtaa gcgtaatttc atcgagatac
    56101 agcgcatcgc tgtcaatctc attgctggtc accggctgca gagtaaagct catctcttct
    56161 tcaccgggga taagccgata caactggcgt tcaacggtca ccggaatgtt actttgttga
    56221 gccgtttttg ccggttcacg ccagcggacc tgcacatttt gcggcgataa ttcgtcacca
    56281 aaagagagaa tgtccggcac accctgacca acccaacgcc agtcttcgcc ccctccagtt
    56341 aacttatgtt tagcccatgc gcccgcaggc gcaggcaaaa caactggagg cattgtcgcc
    56401 atatatttcg ccagccagtt catggccagc gcgcgctcaa tagtggattg ctcagcggtc
    56461 aaaccattaa aatagcggcc gtctgggtag cgtcaccacc gctattgagc agcacaaccg
    56521 tgtgcgccag cggctgattg ctgtttgccg ctttatccca cgcatattgc tgaacctgtg
    56581 gcatcaccgt ggacttcaac tgcgcttttt tcagcaacgt catggttaag acgtttgcca
    56641 ccgcatctgc cagtggagat tccggtgtat cgaggatcag gctgtcattg atatcgcgga
    56701 tgtcttcctc agagaaatct tcatctttag ttccgcgccg ggcgatagct tcatcccccc
    56761 ctttcaacaa cgttttgcac ggcagattca tctcctgtgc ccacgccagc accagcgccc
    56821 gatgcaataa cggcatgtta tccgcctgct cggcataact gtcgagcata tgctgccagt
    56881 attccggttg ttgcgttacg ccgagcgcct ggctggcctg ccaatcggcg taccatgccc
    56941 atgccgtaag gaaggcgtca ccattgccat cttcacccca ccaggtaaag cgcgctccgg
    57001 gccccgccag ttgcatcagc cgcagacggt tatcctgaat catctgacga atgtcgttag
    57061 cggcggcact ttgatgatcg gcaagcgaac gccaggcgag actgagcggg atcagacggc
    57121 taccggtgtt gatcacccca ccccacggtt catcgactaa cgcatcaaga ttgttgcgga
    57181 aaatctcctg cggcgtttca ctactttgta gccggatatt actcgcctgc tcgggcaaca
    57241 tcagcgcgtt ctcgccaccg ccgagcatga catttttctg ctgttcaacg ggccagctgt
    57301 tgtccacaaa agacagtttt gtgctaatgc tgtcctgcac ttgcccattt tgttgcagtt
    57361 ctgcacttaa caagccagat tgctgaatat tctgcgtcag cgaaatataa ttcgcccctt
    57421 tgtgcagcgt cagcgtctgg cgcatctcag cgcctgcaaa tttagtcacc agcgccaccg
    57481 gttcgttatc ctgctgactg aagataaaca gtcctgccgc cggtttgtcg cccacgcgat
    57541 acaccgttgg catactccac ttcatgtaga gatttttttc cgaacgcaga taagcacgcc
    57601 cctgcccgac cagcccgtcg ccgttcatcc cacgcgcggt gatacgccag cgggttaacg
    57661 aatcaggcat caggaacgtg aaatacgctt tgccttgttt atcggttgtg agtgacggca
    57721 tccatgccgc ggtatccacc tcttcacgcc gtggacgttc aagcattttt actcgccgct
    57781 cgctgcggtt agtcgcgcca ggcgcaaccg gctcgctgga gagcgcctgg tcatagctga
    57841 taaacgacag actggagctg gtacgcacat tgttacgccc cagcggatag aaaaatttgc
    57901 cgatattcgg cgcgatttct ggttgcagcg cgtagatcat ttcatcgacc acgcctaccg
    57961 ttagctgcgc agaaacaggt ttacctttca gcgacgaggt taat
//
LOCUS       DADWTH010000016        55740 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.016, whole genome shotgun sequence.
ACCESSION   DADWTH010000016 DADWTH010000000
VERSION     DADWTH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.016"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            77..865
                     /gene="modE"
                     /locus_tag="JNY02_000957"
     CDS             77..865
                     /gene="modE"
                     /locus_tag="JNY02_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBB9432587.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNAATSLQQGQNVTAYFNADSVIIATLC"
     gene            933..2405
                     /gene="modF"
                     /locus_tag="JNY02_000958"
     CDS             933..2405
                     /gene="modF"
                     /locus_tag="JNY02_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBB9432588.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            2666..3682
                     /gene="galE"
                     /locus_tag="JNY02_000959"
     CDS             2666..3682
                     /gene="galE"
                     /locus_tag="JNY02_000959"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBB9432589.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            3692..4738
                     /gene="galT"
                     /locus_tag="JNY02_000960"
     CDS             3692..4738
                     /gene="galT"
                     /locus_tag="JNY02_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBB9432590.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            4742..5890
                     /gene="galK"
                     /locus_tag="JNY02_000961"
     CDS             4742..5890
                     /gene="galK"
                     /locus_tag="JNY02_000961"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBB9432591.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            5884..6924
                     /gene="galM"
                     /locus_tag="JNY02_000962"
     CDS             5884..6924
                     /gene="galM"
                     /locus_tag="JNY02_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBB9432592.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            7127..7879
                     /gene="gpmA"
                     /locus_tag="JNY02_000963"
     CDS             7127..7879
                     /gene="gpmA"
                     /locus_tag="JNY02_000963"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBB9432593.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="JNY02_000964"
     CDS             complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="JNY02_000964"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBB9432594.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAIRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            9414..9794
                     /locus_tag="JNY02_000965"
     CDS             9414..9794
                     /locus_tag="JNY02_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBB9432595.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            9908..10849
                     /gene="zitB"
                     /locus_tag="JNY02_000966"
     CDS             9908..10849
                     /gene="zitB"
                     /locus_tag="JNY02_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBB9432596.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(10846..11565)
                     /gene="pnuC"
                     /locus_tag="JNY02_000967"
     CDS             complement(10846..11565)
                     /gene="pnuC"
                     /locus_tag="JNY02_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBB9432597.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="JNY02_000968"
     CDS             complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="JNY02_000968"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBB9432598.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(12895..12970)
                     /locus_tag="JNY02_000969"
     tRNA            complement(12895..12970)
                     /locus_tag="JNY02_000969"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12935..12937),aa:Lys,seq:ttt)
     gene            complement(12974..13049)
                     /locus_tag="JNY02_000970"
     tRNA            complement(12974..13049)
                     /locus_tag="JNY02_000970"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13014..13016),aa:Val,seq:tac)
     gene            complement(13101..13176)
                     /locus_tag="JNY02_000971"
     tRNA            complement(13101..13176)
                     /locus_tag="JNY02_000971"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13141..13143),aa:Lys,seq:ttt)
     gene            complement(13179..13254)
                     /locus_tag="JNY02_000972"
     tRNA            complement(13179..13254)
                     /locus_tag="JNY02_000972"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13219..13221),aa:Val,seq:tac)
     gene            complement(13391..13466)
                     /locus_tag="JNY02_000973"
     tRNA            complement(13391..13466)
                     /locus_tag="JNY02_000973"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13431..13433),aa:Lys,seq:ttt)
     gene            complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="JNY02_000974"
     CDS             complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="JNY02_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBB9432599.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAHAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(14432..14953)
                     /gene="pal"
                     /locus_tag="JNY02_000975"
     CDS             complement(14432..14953)
                     /gene="pal"
                     /locus_tag="JNY02_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBB9432600.1"
                     /translation="MQLNKVLKGLMIALSVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="JNY02_000976"
     CDS             complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="JNY02_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBB9432601.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="JNY02_000977"
     CDS             complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="JNY02_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBB9432602.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="JNY02_000978"
     CDS             complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="JNY02_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBB9432603.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="JNY02_000979"
     CDS             complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="JNY02_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBB9432604.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="JNY02_000980"
     CDS             complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="JNY02_000980"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBB9432605.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(19394..19687)
                     /gene="ybgE"
                     /locus_tag="JNY02_000981"
     CDS             complement(19394..19687)
                     /gene="ybgE"
                     /locus_tag="JNY02_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBB9432606.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(19687..19800)
                     /gene="cydX"
                     /locus_tag="JNY02_000982"
     CDS             complement(19687..19800)
                     /gene="cydX"
                     /locus_tag="JNY02_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBB9432607.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(19815..20954)
                     /gene="cydB"
                     /locus_tag="JNY02_000983"
     CDS             complement(19815..20954)
                     /gene="cydB"
                     /locus_tag="JNY02_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBB9432608.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(20970..22538)
                     /gene="cydA"
                     /locus_tag="JNY02_000984"
     CDS             complement(20970..22538)
                     /gene="cydA"
                     /locus_tag="JNY02_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBB9432609.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(23387..26065)
                     /locus_tag="JNY02_000985"
     CDS             complement(23387..26065)
                     /locus_tag="JNY02_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBB9432610.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLEPLEPLEPLATLAWTLGFE
                     YHHGLLEKIWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIAD
                     NMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPG
                     LIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENA
                     FWQIALNEDGTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQ
                     CEIIHEAWQSRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLD
                     NQADDHRLRVLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNML
                     NYAALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGI
                     KMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKA
                     GFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISC
                     SETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            complement(26083..27999)
                     /gene="mngA"
                     /locus_tag="JNY02_000986"
     CDS             complement(26083..27999)
                     /gene="mngA"
                     /locus_tag="JNY02_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBB9432611.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNA
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            28169..28891
                     /gene="mngR"
                     /locus_tag="JNY02_000987"
     CDS             28169..28891
                     /gene="mngR"
                     /locus_tag="JNY02_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBB9432612.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(28995..29864)
                     /gene="sucD"
                     /locus_tag="JNY02_000988"
     CDS             complement(28995..29864)
                     /gene="sucD"
                     /locus_tag="JNY02_000988"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBB9432613.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(29864..31030)
                     /gene="sucC"
                     /locus_tag="JNY02_000989"
     CDS             complement(29864..31030)
                     /gene="sucC"
                     /locus_tag="JNY02_000989"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBB9432614.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(31124..32341)
                     /gene="odhB"
                     /locus_tag="JNY02_000990"
     CDS             complement(31124..32341)
                     /gene="odhB"
                     /locus_tag="JNY02_000990"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBB9432615.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(32356..35157)
                     /gene="sucA"
                     /locus_tag="JNY02_000991"
     CDS             complement(32356..35157)
                     /gene="sucA"
                     /locus_tag="JNY02_000991"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBB9432616.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(35458..36174)
                     /gene="sdhB"
                     /locus_tag="JNY02_000992"
     CDS             complement(35458..36174)
                     /gene="sdhB"
                     /locus_tag="JNY02_000992"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBB9432617.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(36190..37956)
                     /gene="sdhA"
                     /locus_tag="JNY02_000993"
     CDS             complement(36190..37956)
                     /gene="sdhA"
                     /locus_tag="JNY02_000993"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBB9432618.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(37956..38303)
                     /gene="sdhD"
                     /locus_tag="JNY02_000994"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(37956..38303)
                     /gene="sdhD"
                     /locus_tag="JNY02_000994"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBB9432619.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(38297..38701)
                     /gene="sdhC"
                     /locus_tag="JNY02_000995"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(38297..38701)
                     /gene="sdhC"
                     /locus_tag="JNY02_000995"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBB9432620.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            39082..39189
                     /locus_tag="JNY02_000996"
     CDS             39082..39189
                     /locus_tag="JNY02_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432621.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            39395..40678
                     /gene="gltA"
                     /locus_tag="JNY02_000997"
     CDS             39395..40678
                     /gene="gltA"
                     /locus_tag="JNY02_000997"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBB9432622.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            41069..41635
                     /locus_tag="JNY02_000998"
     CDS             41069..41635
                     /locus_tag="JNY02_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBB9432623.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            41686..44145
                     /locus_tag="JNY02_000999"
     CDS             41686..44145
                     /locus_tag="JNY02_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBB9432624.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQY
                     CSLHLPEHMEDTANRLILPCH"
     gene            44160..44891
                     /locus_tag="JNY02_001000"
     CDS             44160..44891
                     /locus_tag="JNY02_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBB9432625.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            44888..45949
                     /locus_tag="JNY02_001001"
     CDS             44888..45949
                     /locus_tag="JNY02_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9432626.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIHVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            46101..47147
                     /locus_tag="JNY02_001002"
     CDS             46101..47147
                     /locus_tag="JNY02_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB9432627.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(47144..47935)
                     /gene="nei"
                     /locus_tag="JNY02_001003"
     CDS             complement(47144..47935)
                     /gene="nei"
                     /locus_tag="JNY02_001003"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBB9432628.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(47971..48705)
                     /gene="pxpA"
                     /locus_tag="JNY02_001004"
     CDS             complement(47971..48705)
                     /gene="pxpA"
                     /locus_tag="JNY02_001004"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBB9432629.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKETQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(48695..49627)
                     /gene="pxpC"
                     /locus_tag="JNY02_001005"
     CDS             complement(48695..49627)
                     /gene="pxpC"
                     /locus_tag="JNY02_001005"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBB9432630.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(49621..50277)
                     /gene="pxpB"
                     /locus_tag="JNY02_001006"
     CDS             complement(49621..50277)
                     /gene="pxpB"
                     /locus_tag="JNY02_001006"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBB9432631.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(50300..51043)
                     /gene="ybgI"
                     /locus_tag="JNY02_001007"
     CDS             complement(50300..51043)
                     /gene="ybgI"
                     /locus_tag="JNY02_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBB9432632.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLENDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            51314..52795
                     /gene="dtpD"
                     /locus_tag="JNY02_001008"
     CDS             51314..52795
                     /gene="dtpD"
                     /locus_tag="JNY02_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBB9432633.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(52945..54363)
                     /gene="phrB"
                     /locus_tag="JNY02_001009"
     CDS             complement(52945..54363)
                     /gene="phrB"
                     /locus_tag="JNY02_001009"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBB9432634.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(54360..54869)
                     /locus_tag="JNY02_001010"
     CDS             complement(54360..54869)
                     /locus_tag="JNY02_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBB9432635.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            54998..55315
                     /locus_tag="JNY02_001011"
     CDS             54998..55315
                     /locus_tag="JNY02_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBB9432636.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGMARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(55370..55690)
                     /locus_tag="JNY02_001012"
     CDS             complement(55370..55690)
                     /locus_tag="JNY02_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432637.1"
                     /translation="MPGYRTWIEISLIDELKPSYPFRLEEYAMIGHSPYANECNKDEA
                     KFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELKTRLSTLHVNLEQFINSSEDD
                     EYTL"
BASE COUNT        13636 a        14859 c        13644 g        13601 t
ORIGIN      
        1 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
       61 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
      121 attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag
      181 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
      241 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg
      301 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
      361 ccagcaaaaa gcctttgatg tgttaagtga tgatgacgcc ctgccgctga acagcctgct
      421 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat
      481 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
      541 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg
      601 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt
      661 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
      721 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
      781 aaatgcagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
      841 cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
      901 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
      961 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     1021 atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg
     1081 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     1141 tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat aacaccgata
     1201 tgctcggccc tagcgaagat gacaccggac gcactacggc tgagattatt caggatgaag
     1261 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     1321 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     1381 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     1441 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actttggtac
     1501 tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc gtgctggcgg
     1561 attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc
     1621 aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     1681 cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc gtggtttctt
     1741 ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc gaacactggc
     1801 aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt actggcgatc
     1861 atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa
     1921 ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     1981 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     2041 atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     2101 ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     2161 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     2221 aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg ctgattagcg
     2281 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     2341 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     2401 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt
     2461 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     2521 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     2581 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     2641 taccataagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     2701 tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     2761 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     2821 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca
     2881 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa
     2941 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     3001 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     3061 ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag
     3121 caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat
     3181 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     3241 tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg taggccgtcg
     3301 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     3361 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     3421 caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt
     3481 ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg
     3541 cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     3601 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     3661 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc
     3721 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     3781 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     3841 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     3901 cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     3961 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     4021 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     4081 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     4141 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
     4201 ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt
     4261 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
     4321 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
     4381 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
     4441 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
     4501 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
     4561 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
     4621 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     4681 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     4741 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     4801 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     4861 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga
     4921 tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     4981 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
     5041 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
     5101 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     5161 attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     5221 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
     5281 cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc
     5341 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
     5401 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
     5461 agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
     5521 cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
     5581 cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
     5641 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
     5701 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
     5761 cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
     5821 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
     5881 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
     5941 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttcc
     6001 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg
     6061 gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta taccaaccgc
     6121 atcgccaata gtcgttatac ctttgacggt gaaaccgtga cgctttcgcc aagccagggc
     6181 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
     6241 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
     6301 ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
     6361 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
     6421 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
     6481 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
     6541 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
     6601 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
     6661 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
     6721 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
     6781 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
     6841 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
     6901 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
     6961 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa taaccttaca
     7021 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
     7081 ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg ctgtaactaa
     7141 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
     7201 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
     7261 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
     7321 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
     7381 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga
     7441 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga
     7501 actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga
     7561 gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
     7621 gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg
     7681 taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga
     7741 gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
     7801 gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa
     7861 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga
     7921 gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc
     7981 gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
     8041 aaaccttacc cgcgacgcgc ttttactgca ttcgccagtt gacgtaacag agcatcggta
     8101 tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta
     8161 tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt
     8221 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
     8281 ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc tgctttgttc
     8341 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
     8401 agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg cccccattta
     8461 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
     8521 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
     8581 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
     8641 agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta
     8701 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
     8761 atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag
     8821 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgaatg
     8881 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
     8941 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgagc aaccgtattc
     9001 gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag taactctttg
     9061 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gtcgccatac
     9121 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
     9181 tttacactat gaacggatta atcctagaat tagcaagaaa aacgccagat tgtcgcgaaa
     9241 aacgagatct ttcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
     9301 tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca
     9361 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
     9421 tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac
     9481 tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg
     9541 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
     9601 taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg
     9661 gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata
     9721 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
     9781 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
     9841 atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
     9901 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
     9961 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg
    10021 tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac
    10081 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    10141 ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct
    10201 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    10261 ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    10321 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    10381 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    10441 ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    10501 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt
    10561 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    10621 tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    10681 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    10741 tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga
    10801 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    10861 gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa
    10921 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    10981 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag
    11041 aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg
    11101 ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg
    11161 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    11221 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc
    11281 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    11341 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    11401 aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    11461 cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcccgcccc
    11521 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    11581 taacgttacg ccttattatt cgttatccac gtagtgtaac cgcaaaatcc agcatacgat
    11641 tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc
    11701 cttcctgttc taatgcctct gcgatagcct gaaggccatt catggccatc cacggacaat
    11761 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    11821 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct
    11881 gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    11941 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    12001 cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc
    12061 agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg
    12121 cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg
    12181 aagttaccac ccaatctgcg cgcgttttta ccgcagcaga agtgttggcg tagacgacga
    12241 cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat
    12301 ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    12361 tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg
    12421 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    12481 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    12541 gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    12601 gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt
    12661 ttacaggctt aacgttaaaa ccgacattag cgtaacattc gcttttatcg gtgtaatttg
    12721 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat
    12781 tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt
    12841 gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt acattggtgg
    12901 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
    12961 ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgcccccctc cttgtaaggg
    13021 aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc
    13081 accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    13141 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
    13201 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgacact
    13261 acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt
    13321 ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acattttact accgcttcca
    13381 ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    13441 tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt ttgatatccc
    13501 gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa tttcgatgca
    13561 gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt
    13621 gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag
    13681 ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct
    13741 gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc ggatagtttt
    13801 tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg tagtttaact
    13861 gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt ttgatgaaat
    13921 tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc gcaatagctg
    13981 cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc gcagtaccag
    14041 catcagctgt cggcgtcgtt gatgccgccg caccgctttg atcgctgctg gttgattgcg
    14101 ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt tgccgctcca
    14161 cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa tcaatatcgg
    14221 attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga gcattagaaa
    14281 tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta ctgattggtg
    14341 catgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga ctcaatagtt
    14401 gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca cgacggtttt
    14461 tggagtatgc tgcttcgtca tgacccagta ctgcaggttt ttctttaccg taagaaacga
    14521 tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg gcgttcgcac
    14581 gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta
    14641 cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc agcatttgag
    14701 cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg ttctgctgca
    14761 gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg ccgccgttcg
    14821 catccatacc agtgccagca cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt
    14881 tggaagaaca tgccgcaatt gccataacag acagagcaat catcagccct ttcagcactt
    14941 tgttcagttg catttcaatg attcctttac tattcaatta attattatca cagatacggc
    15001 gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt gaaacgccca
    15061 tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta gattaccata
    15121 gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag aacttgtacg
    15181 cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga gctgaccatt
    15241 accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc ttcccaggta
    15301 atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg accggcctgg
    15361 tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt attgttactg
    15421 cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta caggttcaga
    15481 ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca
    15541 ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag cgtctgaata
    15601 accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc gtctggtgac
    15661 cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt gtaaccgtca
    15721 tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac aacgtaggca
    15781 atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc atcactggcg
    15841 gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg agcaagtaca
    15901 gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga accatccgga
    15961 ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca tgcagctggt
    16021 tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa cggattaaat
    16081 ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg tgccgcacca
    16141 ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac accgctgtcg
    16201 atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa accaaatgct
    16261 actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca cgataattta
    16321 gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca accggtgcct
    16381 gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt
    16441 ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag tttagctgct
    16501 gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc cagtaacata
    16561 ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga cgatgcgtca
    16621 tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat atcggcccct
    16681 gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt ccctttcgcc
    16741 cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat atcatctgcc
    16801 tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc tgccgctttt
    16861 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
    16921 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt cttatcagct
    16981 gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc agcagcttca
    17041 gttgccgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc tttcttcttc
    17101 agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
    17161 gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg cagcttcttc cgcagcttta
    17221 gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa ctcggcctgt
    17281 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
    17341 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
    17401 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
    17461 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
    17521 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
    17581 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
    17641 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
    17701 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
    17761 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
    17821 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
    17881 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
    17941 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
    18001 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag
    18061 caacagcacc agcagtacgt ccagcaacgg tacaatgttg atttcggact tgagatcgcg
    18121 acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa
    18181 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
    18241 tttgtttacg cgctggttga gacggttata agccattacc gccggaattg cggcgaacag
    18301 accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
    18361 ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg taccaaacag
    18421 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
    18481 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
    18541 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat
    18601 ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga ggcgagagag
    18661 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg ctgcgttaag
    18721 aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa
    18781 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
    18841 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
    18901 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
    18961 tgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
    19021 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
    19081 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
    19141 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
    19201 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
    19261 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
    19321 tgagggcaag ttaagggagc gtaaaaattt atgggcctgg agcggcccat aattttttaa
    19381 agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa
    19441 gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa
    19501 tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg
    19561 tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca
    19621 cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc gcgataatct
    19681 tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc
    19741 ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat
    19801 ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata
    19861 cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc
    19921 accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc
    19981 agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct
    20041 gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc
    20101 atacgtgcag tcaggatggt cagcagcggc agaaccacac ccagtgccgg aatagcccac
    20161 agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta
    20221 ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata
    20281 ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc
    20341 tgagccgttg cacgggtacg caggtgcagt tcgcccccgg tacgcatttg cagataggtt
    20401 gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta
    20461 agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt
    20521 acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca
    20581 atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag
    20641 tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag
    20701 ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg
    20761 atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc
    20821 tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca
    20881 gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact
    20941 tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac
    21001 tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaacttgaac
    21061 attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag
    21121 aagatgagat cgcctgcggt cagtgacgaa ttcgccacag ctgtcggcag cacttcaccg
    21181 atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac
    21241 ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg
    21301 cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc acacgccacc
    21361 atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt
    21421 tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg
    21481 taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca
    21541 gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc
    21601 tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg
    21661 cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg
    21721 ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc
    21781 agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca
    21841 gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg
    21901 aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc
    21961 acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc
    22021 tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg
    22081 ttttgcatcc agccgttcgc aatcagaatc cacagtgcgg acaggtttga accgagcgcc
    22141 accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac
    22201 agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca
    22261 ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc
    22321 atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac
    22381 ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc
    22441 aggaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag
    22501 gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat
    22561 gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg
    22621 tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca
    22681 aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca
    22741 ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatagctaa
    22801 attgccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat gcccatttcc
    22861 aagacttata cattgataaa tatcaatttc tacaaattat gttaatcatg tttcaacaag
    22921 gtaactttag tgacatttat gtttaaaatg tgtgaattat aagactgaaa ttagtttcgg
    22981 ttggcaatta aagataaaaa gagacgaagc aaaaacagca cggttgacct taattctttt
    23041 tttggtcact ggctaatacg acgcagagtt tcaggattat taataaaggc ataaatctcc
    23101 tccccgccga tattaacatt taattaactt ataattatat tataattaac tcacccgggg
    23161 ttattatttt gttaaaaata aagccacata tacgtagctt gagagtgaag ataaacgcaa
    23221 aattacctga tgcgctacgc ttgccagtgt ctacatcatc tctgcaatat attgaatttg
    23281 cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt
    23341 tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag gcaagccggt
    23401 aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa ccttgatttt
    23461 cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc tcgcgactga
    23521 aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt aaaatcactt
    23581 cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg ggcattttca
    23641 acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc gcatcccatg
    23701 ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga gctacaccag
    23761 cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc agaccacgta
    23821 gttgtgagtc cgggactggc attttaattc ccgaaggccg cctaggcctt aaaagcagat
    23881 cttctttccc cagtaagccc acgccgcgca gcaacgtaat ggcaaaggtt ttcttctctt
    23941 caccgatgac ttcaaattca cgtagccctt cgctaaagac agccatgccg ttacgccctt
    24001 cctgtaaggc tgcatagttg agcatattcc ataccggaac cggcgcttct ttccagcctt
    24061 cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa ccaaactggg
    24121 tatctgccag aacactatcg gtgttaaaag atgtagggat caggacgcga aggcgatgat
    24181 cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgttgtta tgactaagag
    24241 tgacaaccaa ctcgacacct actctgccag tggattgtct tgcgctgcgt tgttgcagat
    24301 tacgcggcac cgccatttca tagcggataa cagccctgct ctgccaggcc tcatgaataa
    24361 tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt gcgggtgaat
    24421 agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg tcatagcgca
    24481 caccgctatc tttatctacc agttgtagcg ttccatcctc attgagcgca atttgccaga
    24541 aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg ccaggctgat
    24601 tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg atctgtatat
    24661 caaactccat aaagggatcg taattaccgt aatgaactat ttgccgatcg attaggcctg
    24721 gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga tcgtctcgta
    24781 gattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc cgcggccagg
    24841 gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg cggcatgttg tcggcgattt
    24901 tacgcatata gaaacgcagc agattatccg ccatgtcttc agccagttcg aagcgggcga
    24961 cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg gcatgatttt
    25021 ttaagatctc tttccatatt ttctccagca agccgtggtg gtattcaaaa cccaacgtcc
    25081 aggccagcgt tgccagcggt tccagcggtt ccagcggttc cagcggttcc agcggttcca
    25141 gcggttccag cagattaaca atcttatttt caatacgtgc gtgggcaact ttgatatcca
    25201 tgcgcgtgga accgatggtg cgatgcacgc gcatatattt gccatcaata aattcccctt
    25261 tcagggttgc cagattatct cgctgcgctt cgatcttctc aaatacctct tcaaagcggc
    25321 tcatcacaaa ctttcgttga ggataaattt cgcgcagttt ctccatcact tcgaagatat
    25381 tttgctgcaa tggcatctga tcatgcccat tcggcagcaa aatctctttg gttacagacg
    25441 ctttttccag cacgtcaaaa taactgtcga ggcgtttgcg gaatccgttt tcgtccgcag
    25501 gtaagtactt accgatggca taacccaacg gcatcacctg cgccgtcact tcgctaccgt
    25561 cactgctttg ccacaaaaac tcggttttat ccgtaccgtg gcgctcagaa catccacgcc
    25621 agaacatggt gcaggtaatg ccaaatccat tgtagatatg cggcagttgc ccggacatgc
    25681 caaaggaatc gggtaaataa cctattttca tcggctcgcc aaacgcgagg cagtcacgca
    25741 ttccgtacat cagattacgg acgatggatt ccgcagaaac aatcgtggta tcggtctggg
    25801 tataccaggg gccgataatc aacttgccgg cttctaccag tttcttcacg cggtctttgt
    25861 tttccggttt caccgcgaaa taatcttcga ggatcgccgt ttgcccgtcg agtacgtaat
    25921 atttgtattc gttgtcctgt tccagtcggc acaggatctc ttccatatta ttgaccagca
    25981 gaatgcgtga ctcttcggtg gtgaaatacc actcccgatc ccagtgcata tgcggggtga
    26041 tgtgaacgcg agataccgct ttcatcgtcg tttccttttt gtttattgca ttacgccatc
    26101 agtcagatag ttgccatgct taaccgcgtg acgccgccag atcaggagaa ttgcagtcga
    26161 gattgcagcc cccaccaatg ccgcaccaaa ccagccaatt gccgccataa caccgcccgc
    26221 gccattatca tgaagtaaaa agagcgagaa aatgccagca ccgggtgtcg aaaggccgat
    26281 attcatcgca ccgacaatag cgcccgttac catagagccc agcacaaacg aaccaataac
    26341 ccgcagcgga tcttcaatcg ccatcgggat cgccccttcg gtaatacctg ccagccctaa
    26401 cagccaggtg gatttcccgg tctcaatttc aaactcttta aacaggcgcg gtgcgagcat
    26461 cgtggaagcg gttacggtaa atgccgaaac cattttgacg gaggcgaaaa tggcatacgg
    26521 gccgtaaacg ccgttcgcca ttgcgcccag gcagaatgca taagcggctt tattcactgg
    26581 ccctccaagg tcaaaggaac acataaaacc gagaatggca cccagcaaca gcgcgttact
    26641 tcctgacaga gcgttcagcc aggcggtaag cgagttattg atccaggcga caggttcccc
    26701 caccacaaac agcatcagac tgcccgctcc caatgtaccg agcaccaggt agagataaaa
    26761 agtcaggaag ccattgaatt tactgctaag acgcaagtga tttttcatcc agcgtatcaa
    26821 gtaaccggct atcaatccgc caacgaccgc gccgagaaac ccggagccga tcatgttggc
    26881 ggcaagtcca gccgcaaagc ctggcgctaa cgccggttta tctgccagcg aataggcggt
    26941 ataggctgcc agcaccggta ccatcaaaat tccgagcagc ccgccgccca gtttgcggta
    27001 catccacagc caggagtttt cttcattaaa cagatcttgt agcccgaaga tttgcgacag
    27061 taataccgcg accgccagca ccgtcccccc cgcgacaatc aacggtacgg caaaagagat
    27121 cccgctcaac agtgcctgtt tcagctccgt tttgacgctt ttcaccggtt gcgtatcttg
    27181 ctgtacggta cgcgtctcat cgccacgctc gagggtaagc gattgttgga tcaacgcttc
    27241 tgcatggcga atcggctcgg caacaggcac agaaagcgca ggaatgccgt taaaacgctc
    27301 actctccttg atggcgactt cagccgcaaa aatacaggca gttgcactat tgagttgatc
    27361 cgccgttaaa cgcccttcaa taccgttagc accttgcttt tcaacgtaga cattcacgcc
    27421 gagtttgcgt ccggcttttt ccagatactc cgcagccatg taggtgtgag caatacccgc
    27481 cgggcaggcc gttacgcaaa cgatagttgg cgcgttggaa aaagaggcag aaggttgcgt
    27541 gcctcccttg tcatccagcg ccgagagcaa ttcatcaggc gtcgtcgccg actgtatacg
    27601 cgcccgaatc tcatcatccg ccaggcgcgt ggtcagcgct gtcagcagtt gcatgtgcgt
    27661 agtacccgct tcattggggg gaatcgccag caacaccact aaatcaaccg cttccgggcc
    27721 atccacgctt tcccactgaa gcggctcgct gagtgtggcg accgcaaacg ccgcttcttt
    27781 taccgcagca gttttgccat gcggcacagc caacccttca cctaaggcgg tcgggccaag
    27841 gctttcacgg cgatacactt ctttcagaaa ttgctcagta ctggaaattt tccccagagc
    27901 agcaagacgt tgagtcaacg cgtgaatggc ctcttcacgg ctggtaaagc gcgcattcag
    27961 acacaacgca tcgcggtggg tcagagtcgt caggttcatc atgatcctca cttgatcgtt
    28021 tttatagtcg cgccagtgtg cccgataaaa caataccatt gcgtaatcaa tttcacttga
    28081 ttgaaattat ttgcattgta tttgtattat ttacaggatg aatttatcgg gcataatggc
    28141 taaattcgta ttaatgagat gctaaataat gggacacaaa cccttatacc gacagattgc
    28201 tgatcgcatt cgtgagcaaa ttgcgcgtgg cgagcttaaa cctggcgatg cgttacctac
    28261 cgaatcggca ttacaaacag agtttggcgt cagccgggtt acggtgcgtc aggctttgcg
    28321 ccagttagtc gagcaacaga tcctcgaaag cattcagggc agcgggactt acgtcaaaga
    28381 agagcgggtc aattacgata tttttcagtt aaccagtttt gatgaaaaac tgtcggaccg
    28441 tcacgtcgat acacacagtg aagttctgat atttgaagtg attccggctg acgattttct
    28501 tcagcaacag ctacaaatca ccgtgcagga tcgtgtgtgg catgtgaagc gcgtgcgcta
    28561 tcgcaagcaa aagccaatgg cgctggaaga aacctggatg ccgcttgcgt tgttcccgga
    28621 tctcacctgg caggtcatgg aaaattcgaa atatcacttt attgaagaag tgaagaagat
    28681 ggttatcgat cgtagcgaac aggaaatcat tcctctgatg ccaaccgaag agatgagccg
    28741 tctgcttaat atcagccaga caaagccgat tcttgaaaaa gtatcgcgtg gatatttagt
    28801 tgatggtcgg gtgtttgaat atagccgcaa cgcttttaat accgatgact ataaatttac
    28861 cctgatagcg cagcgaaaat catcgcgata agcacaaaaa aggccatcat acgatggcca
    28921 accatgtcga aaccgacagc ccttcgccat tcctgtagag ggaagcggcg agggctattt
    28981 cttattacag atatttattt cagaacagtt ttcagtgctt caccgatatc cgccaggctg
    29041 cgaacggttt tcacgcctgc ggcttccaga gcagcgaatt tctcatccgc agtccctttc
    29101 ccaccggcaa tgatcgcacc cgcgtggccc atacgtttgc ctttcggcgc agtcacacca
    29161 gcgatgtaac ccacaactgg cttggtaaca tgctctttga tgtacgctgc cgcttcttct
    29221 tcagcgctac cgccgatctc accgatcatc acgatcgctt cggtctgcgg atctttttcg
    29281 aacatttcga gaatgtcgat aaagttagag ccagggatcg ggtcaccgcc gataccgaca
    29341 caggtcgact gaccgaaacc gtaatccgtg gtctgtttaa ccgcttcata ggtcagtgta
    29401 ccggaacggg aaacgatacc cactttaccc ggtttgtgaa tgtgacccgg ctggataccg
    29461 attttgcatt cacccggggt gataacgcct gggcagttcg ggccgatcat acgaacgcct
    29521 gcttcgtcca gtttcacttt cacggtcagc atatccagcg tcgggatgcc ttcagtgatg
    29581 gtgataatca gtttgatgcc tgcatcgata gcttccagaa tggagtcttt gcagaacggt
    29641 gctggtacgt aaataacaga cgcggaagcg ccagtagcag caacagcttc acgcacggtg
    29701 ttgaacaccg gcaggccgag gtgggtagtg ccgcctttac ctggagttac gccaccaacc
    29761 attttagtgc cgtatgcaat ggcctgttct gagtggaaag tcccctggct accggtaaag
    29821 ccctggcaga taaccttggt gtttttatca attaaaatgg acattatttc ccctccactg
    29881 cggcaacaac ctgctgagct gcatccgtca gaccttttgc tgcaataata ttcaggccgc
    29941 tgtcagccag tttcttcgcg ccgagttcgg cgttgttacc ttccagacgt accacgaccg
    30001 gtacgttaac acccacttcc gctaccgcgc cgatgatacc gtcagcgatc aggtcgcaac
    30061 gaactatacc gccgaagatg ttaaccagaa cggctttcac tttgtcgtca gagaggatga
    30121 ttttgaacgc ttcggttaca cgttctttgg ttgcgccgcc gccaacgtcc aggaagttag
    30181 ccggttcgcc gccgtgcagt ttaactatgt ccatcgtacc catcgccaga cctgcgccgt
    30241 taaccataca accgatgtta ccgtccagcg caacgtagtt cagttcccac tgtgcagcct
    30301 gtgcttcacg cggatcttcc tgcgactggt cacgcatttc gcgcagatca ggctggcgga
    30361 acagggcgtt gccatcagcg cccagtttgc cgtcgaggca aatcagatcg ccctgtttgg
    30421 tgatgaccag cgggttgatt tcgatcagcg ccaggtcgcg ctccaggaaa atggtcgcca
    30481 ggcccatgaa gattttggtg aactgctgaa ccagtttacc ttccagaccc agtttgaacg
    30541 ccagctcgcg tccctgatac ggcatcgggc cagtcagcgg atcaagcgca actttatgga
    30601 tcaggtgcgg agtttcttcc gccacttttt cgatttccac gccgccttcg gtggaggcca
    30661 taaagaccac gcgacgggaa ctacggtcta caacggcacc gagatacagc tctttagcga
    30721 tatcggtcgc cgcttcaacc agaatctggt taaccggttg gccattggca tctgtttgat
    30781 acgttaccag acgcttgccc agccagtttt ctgcaaaagc acggatgtct tctttgctgt
    30841 ttacaacttt cacaccgccc gctttaccgc ggccaccagc gtgaacctga catttcacta
    30901 cccacggacc ggcaccgatt tttgaagcgg cttcttctgc ttcgcgcgga gtagtacagg
    30961 cataacccac cggtgctggt aagccatagc gggcaaacag ttgttttgcc tgatattcat
    31021 gtaagttcat gtgttctgtc catccttcag taatcgttat cttttaaacc gtagaccgac
    31081 accctatcca cgtcggtcta cagtgcaggt gaagcttaaa ctactacacg tccagcagca
    31141 gacgcgtcgg ctcttccagc aactctttga tcgttaccag gaagcccacg gattcgcgac
    31201 catcgatcag acggtgatcg taggacagcg ccaggtacat catcggcagg atctcaacct
    31261 gaccattcac cgccatcgga cgatctttga tagcgtgcat acccagaatt gcgctctgcg
    31321 gcgggttgat gatcggcgta gacatcaggg aaccgaacac accaccgttg gtgatggtga
    31381 agttaccacc ggtcagatct tcaaccgtca gcttgccatc acggcctttg actgccagct
    31441 ctttgatttt cttctcgatg tctgccatgc cgagggtatc gacatcacgc agaaccggcg
    31501 tcaccaggcc gcgcggcgta gaaaccgcca tgctgacgtc gaaatagttg tggtaaacca
    31561 cgtcatcgcc gtcgatagac gcgttcactt ccgggtaacg tttcagggct tcaaccaccg
    31621 ctttcacgta gaaggacata aagcccagac ggatgccgtg gcgtttttca aacgcttcac
    31681 cgtactgctt acgcagatcc ataatcggct tcatgttgac ttcgttgaac gtggtcagca
    31741 tggcggtgga gtttttcgct tccagcagac gctctgccac acgcttacgc aggcgagtca
    31801 tcgggacacg tttttcacta cgtgcagcca gagccggttg cgccgccgga gcagccgctg
    31861 ccggagcaga ctctttcgcc ggggctttcg ccagatgttt ttccacatct tcacgagtca
    31921 gacgaccacc cacaccggtg cctttaatgg cgctggcgtc gagattgtgt tcagccagca
    31981 ggcgacggat cgccgggctt aacgcatcgt tgttttgctc ttccagagac gcctgctggc
    32041 gttgcgccgg agtggacgct ttctcttcag atttggcgct ggtttcttta ccggcgctgt
    32101 tgccttcacg caggcgacca aggatctgac gagacgttac cgttgtacct tcatcttcca
    32161 gaaccgcatc cagaatgccg tctgctgatg ccggtacttc cagtaccact ttgtcagttt
    32221 cgatttctac cagcacttca tcacgtacga ctgcgtcgcc gggtttttta tgccaggttg
    32281 cgacggtggc atcggctacg gattcaggca ggtcagggac cagaatatct acgctactca
    32341 ttgtgtatcc tttatttatt cgacgttcag cgcgtcatta accagatctt gttgctgttt
    32401 ctggtgaacg gacatatacc ctaccgccgg agaggcggag gccgggcggc ctgcataacg
    32461 cagagaagcc ccaaacggaa tcacttcacg gaaatgatgc tggctgcagt accatgcgcc
    32521 ctggttgagc ggctcttcct ggcaccagac aaaatccttg acgtgagcaa actgctgcaa
    32581 cacttcctgc atcgctttat gcgggaacgg gtagagttgc tcgatacgca caatggcgac
    32641 atcgtgttga ttgttcttac gacgctgttc cagcaggtcg taataaacct taccagaaca
    32701 catcactacg cgcttcacgc ccttcggatc aagttcgtcg atttcaccga tggctggcag
    32761 gaaggtgccg ttcgccagtt cttcgaggct ggaaaccgcc agcggatgac gcagcaggga
    32821 tttcggcgac atcacgacca gcggacgacg catcccgcgc agcgcctgac gacgcagcat
    32881 gtggtaaacc tgtgccgggg tagacggtac gcaaacctgc atgttttgct cagcacaaag
    32941 ttgcagataa cgttccagac gcgcggagga gtgctccggc ccctgccctt cgtaaccgtg
    33001 cggcagcaac atcaccagac cacacatccg gccccatttc tgctcgccag aggagatgaa
    33061 ctggtcgata accacctgcg caccgttggc gaagtcaccg aactgcgctt cccagatggt
    33121 cagggtgcgt ggttctgcgg tggcataacc atattcaaac gccagcacgg cttcttctga
    33181 cagtacggag tcccagacac ggaacgcgcc ctgaccgtta tggatgtgtt gcagcggcgt
    33241 gtaagtggaa ccgttagact ggttgtggat caccgcgtgg cggtggaaga aggtaccgcg
    33301 accggagtct tcacccgaca ggcgaaccgg aatgccttca tcaaccagcg tggcgtaagc
    33361 gaggttttcc gcaccgcccc agtcgaacag tttctcaccg gctgccatcg cctggcgatc
    33421 gccataaatc ttggcaacgc gagactgcat ttcaactgct tctggtaccg tactgatacg
    33481 tttagccagt tcctgcaggc gcttcatctc aactttgttc gggtactctt cgtcccattc
    33541 gtggttgagg tacggcgacc aggtgaaaga gtgcatgttc atcggacgcc actctgccac
    33601 tacgcaatcg ccagcatcca gcgcatcgcg gtacaggtta accatctcgg tggcatcttc
    33661 cagcgtcgcc actttttcct gctccagctt gtcagcgtag attttgcgcg gtgtcggatg
    33721 ttttttgatt ttctgataca tcagtggctg ggttgcgctc ggctcgtcgg cttcgttgtg
    33781 gccgtgacgg cggtagcata ccaagtcgat gaagacatca cgtttaaagg tgttacggaa
    33841 atcgagcgcc agacgggtca caaaggcaac ggcttccgga tcatccgcgt taacgtggaa
    33901 aatcggtgcc tgaaccatct taccgatatc agtacagtac ggtgtagaac gggcatccag
    33961 cgggttagag gtggtgaaac caacctggtt gttgataacg atacgtaccg taccgccaac
    34021 ttcataacca cgcgctttcg acatgttcag agtttcctga accacgccct gcccggtcac
    34081 tgcggcgtca ccgtggatgg tgattggcag cactttgttg ctgctcggct catcaagtct
    34141 gtccagacgg gcacgaacag agccgataac gaccgggctt acaatctcaa ggtgagacgg
    34201 gttaaacgcc agcgccaggt gcaccaggcc gccatcggtc tggaagtcag acgagaagcc
    34261 catgtggtat ttcacgtcgc ctgtgccgag gtgttcttta tgtttaccgg cgaactcgtc
    34321 gaacaagtct tgcggttttt tacccagcac gttcaccagc acgttcagac gaccacggtg
    34381 cgccatcccg agaaccactt cgcgggtgcc gctgttgcca gcgtggcgga tcatctcttt
    34441 aagcatcggg attaacgcgt caccgccttc cagcgagaag cgttttgcgc cagggaattt
    34501 tgcgccgagg taacgctcaa ggccttcagc ggcggtcagt tcgcttaaga agcgtttttt
    34561 ctcttcgcta ttgaaagtcg cgcgaccaga ctcgatacgc tgttggatcc agcgtttttc
    34621 ttcggtgctg gtgatgtgca tatactcggc accaatcggg ccgcagtagg tttgcttgag
    34681 ggcggaaata agctcgccga gtttcatggt ttctttgccg ctggcaaatg aaccgacgtt
    34741 gaaggtttcc tggaagtctg cttcggtcag atcgtggaaa gacggatcca gatcggccac
    34801 tttatcttgc tgccacagtc ccagcggatc tagattcgca tgctggtgac cacggaagcg
    34861 gtatgcgtta atgagctgca ggactttaac ctgcttcaca ttggtgtcag ggtcggagat
    34921 cgttgaagag taacgtgaag cgtctttcgc caggcggcgg aaatattcac gcgtttgaga
    34981 gtggaattga tccggtttga ctcccgtacc aggtaactgc tggaacgtcg aacgccagtt
    35041 agcgtcaacc gagtcaggat cggttaagaa gtcttcatag agctgttcta tccagctctg
    35101 gtttgcgcca gagaggtaag aagagtccaa ccaggctttc aaagcgctgt tctgcatcgt
    35161 gatcccttaa gcatcttttt tatgcttact tcgccgtgga tactaccacg cacagtgatg
    35221 tgcgtgccgg ggttcacttg cgagctcttg tctacgtagt ggctcgcgaa ggaaccttta
    35281 aaaactgtct tagtgtcagg ggcagttttt aaagatttcc tgcatttctt tgttacgcct
    35341 gatgcgctgc gcttatcagg cctgcgggta atgacttgta ggccggataa ggcgttcacg
    35401 ccgcatccgg cactggttgc ctgatgcaac gcttgcgcgt cttatcaggc ctacagttta
    35461 cgcattacgt tgcaacaaca tcgacttgat atggccgatg gcgcgcgtcg ggttcagccc
    35521 cttcggacat acactgacgc agttcatgat gctgtgacag cggaatacgc tgaatgcatc
    35581 gctcaaaccg tcgaggcggc tgtcagtctc ggtatcacgg ctatcgatca ggaaacgata
    35641 tgccgctaac aagcctgccg ggccgataaa cttatcggga ttccaccaga aagacggaca
    35701 agaggttgaa caacatgcgc agagaataca ttcatacagc ccgtcgagtt tttcgcgctg
    35761 ctctggcatc tgtaaatgct cgcgagctgg cggattttgt ccattattca acaggtaagg
    35821 cttaattttc tcatattgcg catagaattg tcccatgtct accaccaaat cgcggatcac
    35881 cggtaaacct ggcagcgggc gaatcacaat cttcttgccc ggctggttga gtgccgaaat
    35941 cggggtaata caggccagac cattcttgcc gttcatgttc agaccgtcgg aaccgcacac
    36001 accttcacgg caggagcggc ggaacgacag gctgggatct ttctctttca gctggataag
    36061 cgcatccagc agcatcatgt cgcgaccttc ttccgcttcc agggtgtaat cctgcatacg
    36121 cggagcatca tcaacatccg ggttatagcg ataaattgaa aactcgagtc tcattttcct
    36181 gtctccgcat tagtaagtac gaatcttcgg cgggaatgcc gggcgcagtt tcggttccat
    36241 gttgacgctt cggcgcgtca tggattccga ctcaggcaga tacagggagt ggcacagcca
    36301 gttttcatca tcacgatccg ggaagtcgaa gcggctatgt gcgccacggc tttcggtacg
    36361 gaagttggca gaaacagccg ttgcatacgc cgtttccatc aggttatcca gttccaggca
    36421 ctcaacgcgc tgggtgttga actcgctgga agtgtcatcc agacgggcat ttttcagacg
    36481 ctcgcggatc actttcaact gctcaagccc tttcgccatc gcatcacctt cacggaagac
    36541 cgagaagtta tgctgcatac attcttgcag cgctttacgg atcgccaccg gatcttcacc
    36601 gttacgatta ttgttccacc ggttcaggcg atccagagac gcttcaacat cagactcgct
    36661 ggcatcgcgc agtgcgccct gctcggcgat agactcttgc agatgcagac ctgccgcgcg
    36721 accaaagacc accaggtcca gcagcgagtt gccgcccaga cggttagcgc cgtgtaccga
    36781 tacacaagcg atttcaccaa cggcaaacag tcccggaaca accacatctt cgcctttctc
    36841 attcacagtc agcgcctgac cggtaacttt ggtcggaata ccgcccatca tgtagtgaca
    36901 ggttgggata accggaatcg gctctttcac cggatcgacg tgagcgaagg tacgggaaag
    36961 ctccaggata cccggcagac gggattcgag aacttcttta cccaggtgat cgagtttcag
    37021 tttcgcgtgt ggcccccacg gaccatcaca gccgcgacct tcacggattt caatcatgat
    37081 ggaacgcgcc accacatcac ggcccgccag gtctttggcg ttcggcgcat aacgctccat
    37141 aaagcgttcg ccatgtttgt tcagcagata accgccttca ccacggcaac cttcggtgac
    37201 cagtacgccc gcaccggcaa tgccggtcgg gtggaattgc cacatttcca tatcctgcac
    37261 cggtacgcct gcgcggatag ccatgccgac accgtcgcca gtgttaatgt gggcgttagt
    37321 ggtggactga taaatacgcc ctgccccgcc agtcgccagc acggtagcgc gggctttgaa
    37381 ataaaccact tcaccggttt cgatgcacag tgcggtacaa cccaccaccg cgccatcctg
    37441 gtttttcacc agatccagcg cataccactc ggagaaaatg gtggtgtggt ttttcaggtt
    37501 ctgctgataa agcgtgtgca acagtgcgtg accggtacgg tcagcagccg ccgcagtgcg
    37561 tgccgcctgc tcgccgccga agtttttcga ctgaccgcca aacggacgtt gatagatacg
    37621 accatcatcg agacgcgaga acggcaggcc catgtgttcg agttccagaa tcgcttccgg
    37681 cccggtttta cacatatatt caatcgcgtc ctggtcaccg atatagtccg acccttttac
    37741 ggtgtcgtac atatgccatt cccagttatc ttcatgggta ttacccagcg caacggtaat
    37801 gccgccttgc gcagaaacgg tatgggaacg ggtcgggaag actttagaga gcagcgcaca
    37861 ggtctggccg ctctgggaaa tttgcagcgc cgcgcgcata cctgcgccac cggcaccaat
    37921 cacaactgca tcaaattctc tgactggcaa tttcatcaca ccccccacac cacaacgaat
    37981 ccataaatca cgtaaaccac cagtgcaacg acaatcacca gttgcagcat caggcgcaag
    38041 gccagcggtt taacgtagtc ggtcaacacc tgccacatgc cgatccaggc atgaatcaag
    38101 atagaaaaca gcgccagcag ggtgaacact ttggtgaacg cagaggcgaa gaaaccgatc
    38161 cagacttcat atgtcagttc gccactggtg gcgaaaaaac cgaccatata aatgatgtag
    38221 agcgtcagga cgatagcggt agcgcgaacg aggatgaaat catgtacgcc attgcgtcct
    38281 aatgcggagg cgttgcttac catacgagga ctcctgcgag aagtgaaagc acgacagtaa
    38341 taacaaagga gattttggcg gagcgtttac ccgcttcgaa tgtttcttcc agatagccaa
    38401 aatccatcat catgtggcga atacctacga cgacgtgata cgccagagcg gtaaggatgc
    38461 cccacatgat aaatttgacg aagaagctgc ccataatcgc ggaagcttgc tcgaaacctt
    38521 caggggaaga gaggctggta cccagaagcc acagcaggat gcccactgca acaaaggtga
    38581 tcacaccgga aacgcgatgg agaatggacg ctatcgccgt gatggggaac cggatggtct
    38641 gtaggtccag attaacaggt ctttgttttt tcacatttct tatcatgaat aacgcccaca
    38701 tgctgttctt attattccct ggggactacg ggcacagagg ttaactttct gttacctgga
    38761 gacgtcggga tttccttcct ccggtctgct tgcgggtcag acagcgtcct ttccataact
    38821 gcgcgtcatg caaaacactg cttccagatg cgaaaacgac acgttacaac gctgggtggc
    38881 tcgggattgc agggtgttcc ggagacctgg cggtagtata ggctgttcac aaaatcatta
    38941 caattaacct acatatagtt tgtcgggttt tatcctgaac agtgatccag gtcacgataa
    39001 caacatttat ttaattttta atcatctaat ttgacaatca ttcaacaaag ttgttacaaa
    39061 cattaccagg aaaagcatat aatgcgtaaa agttatgaag tcggtatttc acctaagatt
    39121 aacttatgta acagtgtgga agtattgacc aattcattcg ggacagttat tagtggtaga
    39181 caagtttaat aattcggatt gctaagtact tgattcgcca tttattcgtc atcaatggat
    39241 tctttacctg caagcgccca gagctctgta cccaggtttt cccctctttc acagagcggc
    39301 gagccaaata acaaacgggt aaagccaggt tgatgtgcga aggcaaattt aagttccggc
    39361 agtcttacgc aataaggcgc taaggagacc ttaaatggct gatacaaaag caaaactcac
    39421 cctcaacggg gacacagctg ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt
    39481 tattgatatc cgtactctcg gttcaaaagg tgtgttcacc tttgacccag gcttcacttc
    39541 aaccgcatcc tgcgaatcta aaattacttt tattgatggt gatgaaggta ttttgctgca
    39601 ccgcggtttc ccgatcgatc agctggcgac cgattctaac tacctagaag tttgttacat
    39661 cctgctgaat ggtgaaaaac cgactcagga acagtatgac gaatttaaaa ctacggtgac
    39721 ccgtcatacc atgatccacg agcagattac ccgtctgttc cacgctttcc gtcgcgactc
    39781 acatccaatg gcagtcatgt gtggtattac cggcgcgctg gcggcgttct atcacgactc
    39841 gctggatgtt aacaatcctc gtcatcgtga aattgccgcg ttccgcctgc tgtcgaaaat
    39901 gccgaccatg gccgcgatgt gttacaagta ttccattggt cagccatttg tttatccgcg
    39961 caacgatctc tcctatgccg gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc
    40021 gtatgaagtt aatccgattc tggaacgtgc tatggaccgt attctgatcc tgcacgctga
    40081 ccatgaacag aacgcctcta cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc
    40141 gtttgcctgt atcgcagcag gtattgcttc actgtgggga cctgcgcacg gtggtgctaa
    40201 cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt aaacacattc cggaatttgt
    40261 tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg ggcttcggtc accgcgtgta
    40321 caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga
    40381 gctgggcacc aaagatgacc tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa
    40441 cgacccgtac tttatcgaga agaaactgta cccgaacgtc gatttctact ctggtatcat
    40501 cctgaaagcg atgggtattc cgtcttccat gttcaccgtc attttcgcaa tggcacgtac
    40561 cgttggctgg atcgcccact ggagcgaaat gcacagttac ggtatgaaga ttgcccgtcc
    40621 gcgtcagctg tatacaggat atgaaaaacg cgactttaaa agcgatatca agcgttaatg
    40681 gttgattgct aagttgtaaa tattttaacc cgccgttcaa atggcgggtt aatttttata
    40741 tgcctaaaca caaaaaattg taaaaataaa atccactatc ggacattcat atattctaaa
    40801 aaaaaatata tcggtataaa ttataaacaa atcgatgatt tttaatcatc tatcttcctc
    40861 tcagttacat gaataaacca ccaaatatag cgcaccccta taatttccaa tactttattc
    40921 atcaaacatt taattcatac catcaattaa ttacttttac atttaataaa attaataata
    40981 tttttaagaa aaactaaaaa cattcgacaa tcaaataaga tattcttgca ccgatatact
    41041 tttttctttt ctgttaaagg actcagttat gtttaaagga caaaaaacat tggccgcact
    41101 ggccgtatct ctgctgttca cagcacctgt ttatgcggct gatgaaggat ccggtgaaat
    41161 tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg atattgataa
    41221 agaggttgaa ctcggtcagg tgaccaccag ccacattaat cagtcacatc acagcgatgc
    41281 cgttgctgtc gacctgctct tagtcaactg tgatctggaa aactccagca acggttccgg
    41341 tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca ccggcgcaga
    41401 tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtac gtttaatgaa
    41461 taaagatcaa agcaatatcg ttttaggcac cgctactcca gatatcgacc tggctccgac
    41521 ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc aggctacacc
    41581 tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg ctggattata aataagtatt
    41641 actttggcgg cgctatcgcc gcctttcata aaatatttat caggtatgga caccgtgaat
    41701 atttatcgac tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc attggcggtc
    41761 gaattcaatc tgaatgttct cgataaatca atgcgcgacc gcattgatat ttcattatta
    41821 aaggaaaaag gagtcattgc tcccggtgag tattttgtta gcgttgcggt aaataataac
    41881 caaatcagta acgagcaaaa gattaactgg cacaaaaatg acgataaaac cattccgtgc
    41941 atcaatgatt tactggtcga taaatttggc ttaaaacctg aagtccgtca gtcattacca
    42001 ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa tttcgatcaa
    42061 gccaatcagc aactgaatat tagtattccg caagcctggc tggcgtggta ctcagaaaac
    42121 tggaccccac cctccacatg gaaagaaggt gtcgccggtg tcctgatgga ttacaacttg
    42181 tttgccagca gctaccgccc acaggatggc agcagcagca ctaatcttaa cgcctatgga
    42241 acagccggaa tcaacacggg ggcatggcgc ttacgcagtg attatcagtt gaatcatact
    42301 gatagcgatg ataaccatga acagtcaggc gaaatatcac gtacctatct ttttcgcccg
    42361 ttaccgcaat taggttcaaa attaaccctc ggcgaaacgg attttagttc caatattttc
    42421 gacggttttt cttataccgg cgcggcactg gcaagtgacg atcgaatgtt gccatgggaa
    42481 ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac ggtgacgatc
    42541 agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac caggcccatt tatcattgac
    42601 gaccttaatc agtctgttca gggcacactg gatgtcaaag tgacggaaga agatggtcgg
    42661 gtgaacaatt tccaggtttc agcagcatcg acgcccttcc tgactcgtca gggacaggtt
    42721 cgctataaac tggccgcggg tcagccacgg ccctccatgt cacatcaaac tgaaaatgaa
    42781 acctttttta gcaatgaagt ttcctggggg atgctgtcaa acacctcgct gtacagcggc
    42841 ctgctgcttt ctggtgatga ctaccattct gccgcaatgg gtattgggca aaatatgctg
    42901 tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta gccattttga tactcagcag
    42961 gacgagcggg gcttaagcta ccgttttaat tacagcaaac aagtggatgc cactaacagc
    43021 acgatttccc tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta cgccaactat
    43081 ctggatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat cagcttatct
    43141 gtgggccaac cgattacccc actaaacctc aatctttacg ccaacctgct acatcaaacc
    43201 tggtggaatg cagacgcctc cacgaccgcc aacatcacgg caggttttaa tgttgatatt
    43261 ggtgactgga gagatatctc tatttctacg tcattcaata caacccatta cgaagataaa
    43321 gatcgcgaca accagattta cctgtcgatt tcgctcccct tcggtaacgg tggtcgggtt
    43381 ggctatgaca tgcaaaacag tagccacagc accacacacc gcatgtcgtg gaacgatacg
    43441 ctggatgaac gtaatagctg gggcatgtct gccggactgc aatccgaccg tcctgacaat
    43501 ggagcccagg tgagcggtaa ctatcagcac ctgagttcag cgggtgagtg ggatatttct
    43561 ggtacctatg ccgccaatga ttacagttcc gtcagcagca gctggagcgg ttctttcacc
    43621 gcaacccaat atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc acgcctgatg
    43681 gtcagcaccg atggcgtggc agatattccg gttcagggca atctcgacta caccaaccat
    43741 tttggcattg cggtggtgcc gttgatttcc agttaccagc cttccaccgt ggcggtgaac
    43801 atgaatgact tacccgacgg cgtaacagtt gcagaaaacg ttatcaaaga aacgtggatt
    43861 gaaggcgcga taggttacaa atcactggct tcccgttccg gtaaagacgt taacgtcatc
    43921 attcgcaacg ccagcggtca gtttcctccc ctcggagcgg atatccgcca ggatgacagc
    43981 ggcattagcg tggggatggt tggcgaggaa ggacatgcct ggttaagcgg agtcgctgaa
    44041 aatcaaaagt ttaccgtggt ctggggtgat agccagtatt gctcgctcca tcttcctgaa
    44101 catatggaag acaccgcaaa tcgcctgatt ttaccttgtc attaattaag gaaatgaaaa
    44161 tgacatttat gaaaggacta ccccttttgt tgttggttgc cagtctgtgc agccacgctg
    44221 cactacaacc cgatcgcact cgtattgtgt ttaacgctaa tgataaagct accagtctgc
    44281 gggtggataa tcgtagcgat aaactcccct acctcgctta ttcctggctc gaaaatgaaa
    44341 aaggggagaa aagcgatgat cttctggtcg ccctgccgcc gatacaacgt ctggaaccga
    44401 aagcaacaac gcaggtacgg atagtaaagc aagcctcgac cacaaaatta ccgggcgatc
    44461 gcgaaacgct atttttctac aatatgcgtg aaattcctcc cgccccggaa aaaaacagcg
    44521 accatgccgt tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg cgaccggctg
    44581 cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc cagcagggca
    44641 accaactgac actgaaaaat cctaccgcgt attatctgac cattgcctac ctgggacgaa
    44701 atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt agtacggtca
    44761 acacaaacac cggaaattat agcggcagcc agttttatct cggttatatg gatgattacg
    44821 gcgcgttacg catgacgacg ctcaactgca gcggacaatg ccatttacaa gccgtggagg
    44881 cgaagaaatg agtgctggca agggagtgtt gctcgtcatc tgtctgttat ttctgccgtt
    44941 gaagtctgcg cttgccctga actgctactt tggttcatcc ggtggctcgg tagaaaaatc
    45001 agaagcgatt caaccttttg ctgtaccagg caatgccaaa ctcggcgata agatctggga
    45061 atctgacgat attaaaattc ccgtctattg tgacaacaat accaacggca actttgaaag
    45121 cgaacatgtt tacgcatggg taaaccccta cccgggggtg caggatcgtt attatcaact
    45181 cggagtaact tacaatggcg ttgattatga cgccaaccag ggaaaaagcc gcatcgacac
    45241 taaccagtgt atcgacagta agaacattga catttatacc cctgagcaga tcatcgcgat
    45301 gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgtacctt
    45361 tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag
    45421 tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc
    45481 tactgcccaa aacctgaaat atcatattac tggtctggaa aacattcacg tgctggattg
    45541 cagcgtcaat ttttccattt caccggaaac acaggtgatt gattttggta aatttaattt
    45601 gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca
    45661 aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt
    45721 ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga
    45781 aaacgcctcg ccttatacct tcaataaata tgctgaatac gccgatttca ccagcgacat
    45841 gttggtctac gaaaaaacct atacagctga actttcgtcc attgcaggta cccccatcga
    45901 ggctgggccc ttcgatacag tggtgctttt taaaattaac tataactgat ttgccatgat
    45961 gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt
    46021 gattgctgtt attttttcct agcccggctt tctttatgct gtatcgatac accaaagaga
    46081 aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac
    46141 ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcgctatta cttgggccga
    46201 tgatcgccgg gatcatcttc agtatgcgcg gaattactct gcaacttccc cgctccgctt
    46261 ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctcact ggttcgattc
    46321 tcaccacgct ggccgccaac tggccgatcg tgcttgctat tttactggtg acgctgcttt
    46381 ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga aataccggtg
    46441 cctggggttc ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg
    46501 cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc gcgggggccg
    46561 ctgttctggt cacccgcatg atgctgggtg ataacgctga agcggttaac cagcaaatcg
    46621 tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgca gtcgttgccg
    46681 gtacggcggg atgcatgctg cgtcttcctt ccggcacgat gctcatcccg atgctggcgg
    46741 gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga
    46801 tggcgtatat ggcaattggc tggcggattg gtcttggttt cgataaacaa atcttactgc
    46861 gggcactacg cccgctaccg caaatcctgc tgtcgatttt tgctctgctg gctatttgtg
    46921 caggtatggc gtggggactg acccggttta tgcatattga ttttatgact gcctacctcg
    46981 ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata
    47041 tggcgcttat tatggcgatg caaaccctgc gcctgttcag tattttgctg acagggccag
    47101 ccattgcgcg gtttatttca acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg
    47161 gacaccagta aaacgggcgc gaggacaacg tggttttctc aatgatgccg ccacagcgtt
    47221 cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat
    47281 tctcatccac ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg
    47341 cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa
    47401 cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga
    47461 gcagtaatcc tgcaaactga cggttacgaa agcgcggcga caataaccgt tcctttacca
    47521 cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg
    47581 tggtcagttg ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt
    47641 tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct tcgccggtat
    47701 caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaaa ccgttgggaa
    47761 aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt
    47821 gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt
    47881 tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga
    47941 tatccttttt tcattcttta ttaagcgggg ttatgctgcg acaacaatcc cctttttggc
    48001 aaatgtagct cgtaatcggc gggcgaacgc cagagcgtgc tcgccgtcgc catgcaggca
    48061 gaccgtttgc gccgcgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg
    48121 caccatctcc agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg cgcctggctg
    48181 acttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg
    48241 cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccaacgag
    48301 aaccaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgtttc
    48361 tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg
    48421 cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc
    48481 cggcggcagc tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc
    48541 gccaatcgcg acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc
    48601 gcctgcatga aatccacaag caatattggc agaggaaacc agcgttaata gctctgcgtc
    48661 gctggcgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccacg
    48721 ctaattgttc aaaataacgt tgctgatctt gccgcgcttt cagtgcctct tccagtgaac
    48781 actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct
    48841 caatgataca ggcaatacgt ggataaccac cggtggtctg tgcgtcgttc atcaacacaa
    48901 tcggctgccc gttatgcggc acctgcacca cgcccggtaa caaaccgtga gataacagtt
    48961 cgcgatcggt gatgcgtttt aaaatttgcc cctgtaaacg atagcccatt cggttacttt
    49021 gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat
    49081 gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct
    49141 gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca tccttcagta
    49201 aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac cccattaccg
    49261 gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt
    49321 taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcgttat
    49381 catccaaccg cgcttcgcaa cctgcacccg tcagggcaaa ccacccatca gtttcgaact
    49441 caactgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca
    49501 gcaggttagc aatgcgcagc gcaggcatat ccagtgcgcc acagtggctg atacccgact
    49561 ggcgaaaacc gtgacgaccg ccatcctgca cagtggtgta catgcccgca cgaataatct
    49621 tcagcatatt ccctccttct gcggtacaaa tcgcacgctg tctcccggac gtaataagat
    49681 gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc
    49741 acccggcgtt gccagcggat aaacaccagt ctgcggcccg ccgatcccga cagaacctgc
    49801 cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc
    49861 gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg
    49921 caattcaaca acctgttttt cgctcaaccc gcaatacgcc gcgaccaccg ccaaatccgg
    49981 tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc
    50041 ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg
    50101 attacgcaaa atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat
    50161 atccaccaga cgctgcgcca gtcgccagat ccgcttctgg ctagccagcg tcaccggcgg
    50221 ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt
    50281 cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat
    50341 cggtattttc attcagccac tcgctcaatg cgcgaatacc accgcgttca gtggcatggt
    50401 gacctgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc tgttcagaaa
    50461 cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc
    50521 cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata
    50581 acggcttacg tcccagacgc gcttcaatcc aggaggctag ttccattccc ggcacaggca
    50641 tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta
    50701 acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc
    50761 catacaggtt gatatcattc tccagcaacg tttttaaacg gttacgcttc attccgcgaa
    50821 tgaccggaga ctctcctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca
    50881 gacgcactgc ctcatcgagc aaagcctggc tggcggtgac accggtaaca attttttgca
    50941 ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt
    51001 tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat
    51061 tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag ccaatctttt
    51121 gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc
    51181 agctaatgta tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa
    51241 agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa
    51301 ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact atgtcgttgc
    51361 gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct
    51421 caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc
    51481 gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggcaatcg
    51541 catggcggtg atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag
    51601 tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtctgcg gctatggcct
    51661 gtttaaatct aacgtaagct gtctgctcgg cgagctgtat gagccaaccg atccgcgtcg
    51721 tgatggcggt ttctcgctga tgtatgcggc gggtaacgtg gggtctatta tcgcacctat
    51781 cgcctgtggt tacgcccagg aagagtacag ctgggcgatg ggctttggcc tggcggcggt
    51841 tggcatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca ctcatacccg
    51901 cggcgttaac aaaaaagtac tgcgtgcgac aaactttctc ctgccgaact ggggatggct
    51961 gctggttctg ctggtggcaa cgcctgcact gattaccgtg ctgttctgga aagagtggtc
    52021 ggtatacgcc ttaattgtcg cgactattat tggcctgggt gtactggcaa aaatttatcg
    52081 caaagcagaa aaccagaaac agcggaagga gctggggctg attgtgacgc tcaccttatt
    52141 cagtatgttg ttctgggcct tcgcacaaca gggcggtagc tcgattagcc tttatatcga
    52201 ccgcttcgtt aaccgcgata tgtttggtta taccgttccg accgcgatgt tccagtcgat
    52261 taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt
    52321 cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct cttggccttg gcctgatgag
    52381 cgccggattc tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc
    52441 tctgccactg atggtgttag gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc
    52501 ggttgccatg tcgcaaatta cgcgcattga aattccgggc gtgaccggcg tattaaccgg
    52561 catctacatg ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca
    52621 gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat
    52681 tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg tactgatgat
    52741 ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cataataggc
    52801 cagcaccgat gcctgatgcg acgcttgacg cgtcttatca ggcctacgta tttcctgcaa
    52861 tttattgaat ttgcacaaat ttgtaggctg gattaggcgt tcacgccgca tccggcatct
    52921 gggctcgatt gcctgacgcg tctgttattt ccccttccgc gccgcctcat acgctgccaa
    52981 cgtttgtact ctcgcctctt tgtgctcgac tatcggttgc ggataatcca gcttcacacc
    53041 tgctttctgc gcccacttcc acggctcatg caccgctttc cctggtacat cgcgcagctc
    53101 cggtaaccat cgacgaataa actcgccctc acggtcaaat ttctcgccct gagttatagg
    53161 attgaaaata cgaaaatacg gcgcagcatc agtacccgta gaagccgccc actgccagcc
    53221 accgttatta gctgccaaat caccatcaat cagctgcgac atgaaatatc gctcgccttc
    53281 gcgccagtcg atcaacaaat ctttcaccag aaaactggct gtaatcatcc gtagccggtt
    53341 atgcatccag ccagtgctgt taagctgacg catagcggca tcaataatcg ggtatcccgt
    53401 tttgcctttc tgccaggcct gtaaatgtgc gggattgctt tgccactgca cacgatccgt
    53461 ccaggcaata aatggacgat gtttacacaa cgaggggtaa tacgtcatca gatgacggta
    53521 gaactcgcgc cagatcagct cactaagcca gacactaccg gccccaccgt ccagcgcctg
    53581 cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc gataacccgc ccgttgccaa
    53641 gctggcggac aaacggctgg tgccttccac tgccggaaaa tctcgttgtt gctcatactc
    53701 tccagcaccg ttctcgcaaa actgccgtaa ttgcgcaatc gccgcttttt cttccaccgg
    53761 gaaatgcgca gtatcgaaag actgacgagg ataattcagc gtaatggatg gcgcgggctt
    53821 tatcgatccg ctactacgaa cttttggtgc agcgacgcac tccggcatcc cttcccgcag
    53881 ccgtttcagc caggcattct taaaaggcgt aaagactttg tacatctcgt gattgccggt
    53941 catcaccgcc cccggcggca ggatcacgct gtcatcaaat ccttcacaca ccacgttacg
    54001 cagcgctctt tcaacgtcca catcccgcgc tcgctcattc acctcatact gatagttata
    54061 aaaaagatga gtaacgctgt tttccgcgca cacctgttta actatttcga cactggcgac
    54121 aaagtcatcc acttcacgga acaataaagg aatacctttt tccgccagcg cgatttgtag
    54181 cccattcagt tgagcattga taagttcagc ctgacgcggc gacatgttat gcgtctccca
    54241 ctggcgtggt gtagcgatat acagcgccag cacgcgtgca gacgaattgc ggcaggcggc
    54301 agccagtgcg agattatcgt gcagacgtaa atcctggcga aaccaggcca gatgggtagt
    54361 cataaaactc ctgaatggcg caagttaatc acacgcggcc aaagttcgaa ataacgctgc
    54421 cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat cagcggcgca
    54481 agaagtggtt gctcgccacg gcgataactg tcgataagcg cagccagctg ctggcgttct
    54541 gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg
    54601 cgcgggttgg cgggatgaga aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa
    54661 tcatccagat tttgccactg atgaatatca gccacaaacg ggccaagttt gcggtactcg
    54721 ggctgagaat gggccagaaa gacgagttta tagcggctgt gataatcgag aagcgccccg
    54781 cgcgttaagc catgttcttt cagtcgatgt aactcatgaa gggcgaagat acggattagg
    54841 gtgctgtcat caaatcgttg tagattcatt actgctcctg aacggtatac aggagtaagt
    54901 gtagacgttg gtaaatgatg tttgtgaagt taatccgcta gcagggcaag attacgaaag
    54961 cgcgctcccc gcaagcaact aatcaaggta gttaatcatg gctgaacacg atattacgcc
    55021 agaagtcacc atttccaaaa cacagcgccg ctataaagtc ggctatgtca gagcccgcca
    55081 ccaggaccgc aaaaccggta tggcccggta ttatagcctg caccttaagg gcgactggct
    55141 gaaagaggcc ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct
    55201 gaccatcatc gctgacagtg acgaggtgca ggaactgcgg gaggaacttt atcaggttaa
    55261 acaggtagtt aaggggatgc gggatgggat gtttagtgtg ctgaacgaaa gttaaatatt
    55321 aaaaaagctg ggaagattta aaaccttcct agctttttat tctgtcttat tataatgtat
    55381 actcatcatc ttccgatgaa tttataaatt gctccaagtt tacatgaaga gtacttaacc
    55441 tagtttttaa ctcattaagt gaagcgataa tttcatattt tccaacataa tcatatgatg
    55501 gatcgtattc attatctatg tcagaagcta aaaatgatga tattcgtagt ttaaatttag
    55561 cttcatcttt attacattca ttggcataag gtgaatgccc tatcatagca tattcctcta
    55621 atctaaaagg ataagatggt tttaattcat cgatcaatga aatttcaatc catgttctat
    55681 atccaggcat taatggacta atttctttga gcaaaacctt tccacttaat aatgtattga
//
LOCUS       DADWTH010000017        55423 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.017, whole genome shotgun sequence.
ACCESSION   DADWTH010000017 DADWTH010000000
VERSION     DADWTH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.017"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..373
                     /locus_tag="JNY02_001013"
     CDS             <1..373
                     /locus_tag="JNY02_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9432638.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            484..1407
                     /locus_tag="JNY02_001014"
     CDS             484..1407
                     /locus_tag="JNY02_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBB9432639.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVKGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNY02_001015"
     CDS             complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNY02_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBB9432640.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2451..2744
                     /locus_tag="JNY02_001016"
     CDS             2451..2744
                     /locus_tag="JNY02_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432641.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNY02_001017"
     CDS             complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNY02_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBB9432642.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4186..4638)
                     /locus_tag="JNY02_001018"
     CDS             complement(4186..4638)
                     /locus_tag="JNY02_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBB9432643.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHPPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4691..6025)
                     /gene="adeQ"
                     /locus_tag="JNY02_001019"
                     /gene_synonym="yicO"
     CDS             complement(4691..6025)
                     /gene="adeQ"
                     /locus_tag="JNY02_001019"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBB9432644.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6200..7966
                     /gene="adeD"
                     /locus_tag="JNY02_001020"
     CDS             6200..7966
                     /gene="adeD"
                     /locus_tag="JNY02_001020"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBB9432645.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNY02_001021"
     CDS             complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNY02_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBB9432646.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNY02_001022"
     CDS             complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNY02_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBB9432647.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNY02_001023"
     CDS             complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNY02_001023"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBB9432648.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNY02_001024"
     CDS             complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNY02_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBB9432649.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13037..13327)
                     /gene="ilvN"
                     /locus_tag="JNY02_001025"
     CDS             complement(13037..13327)
                     /gene="ilvN"
                     /locus_tag="JNY02_001025"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBB9432650.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNY02_001026"
     CDS             complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNY02_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBB9432651.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPVAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPLGAANTEMVGE"
     gene            complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNY02_001027"
     CDS             complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNY02_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBB9432652.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15788..15877
                     /gene="tisB"
                     /locus_tag="JNY02_001028"
     CDS             15788..15877
                     /gene="tisB"
                     /locus_tag="JNY02_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBB9432653.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16157..17341
                     /gene="emrD"
                     /locus_tag="JNY02_001029"
     CDS             16157..17341
                     /gene="emrD"
                     /locus_tag="JNY02_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBB9432654.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17349..17846)
                     /locus_tag="JNY02_001030"
     CDS             complement(17349..17846)
                     /locus_tag="JNY02_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBB9432655.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPSRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17843..18205)
                     /locus_tag="JNY02_001031"
     CDS             complement(17843..18205)
                     /locus_tag="JNY02_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBB9432656.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18195..18542)
                     /locus_tag="JNY02_001032"
     CDS             complement(18195..18542)
                     /locus_tag="JNY02_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBB9432657.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18651..19064
                     /locus_tag="JNY02_001033"
     CDS             18651..19064
                     /locus_tag="JNY02_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432658.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19111..20553)
                     /locus_tag="JNY02_001034"
     CDS             complement(19111..20553)
                     /locus_tag="JNY02_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBB9432659.1"
                     /translation="MVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCTPARAGLFT
                     GIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWD
                     ADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQP
                     VRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQA
                     MLSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDHGEMMGAHK
                     LISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGEN
                     ILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPN
                     EMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPR
                     PQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20588..22303)
                     /locus_tag="JNY02_001035"
                     /pseudo
     CDS             complement(20588..22303)
                     /locus_tag="JNY02_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22470..23363
                     /locus_tag="JNY02_001036"
     CDS             22470..23363
                     /locus_tag="JNY02_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB9432660.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23360..24174)
                     /locus_tag="JNY02_001037"
                     /pseudo
     CDS             complement(23360..24174)
                     /locus_tag="JNY02_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24174..25790)
                     /locus_tag="JNY02_001038"
     CDS             complement(24174..25790)
                     /locus_tag="JNY02_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB9432661.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26085..26759
                     /locus_tag="JNY02_001039"
     CDS             26085..26759
                     /locus_tag="JNY02_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB9432662.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26798..28459)
                     /locus_tag="JNY02_001040"
     CDS             complement(26798..28459)
                     /locus_tag="JNY02_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBB9432663.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28655..29083)
                     /gene="ibpB"
                     /locus_tag="JNY02_001041"
     CDS             complement(28655..29083)
                     /gene="ibpB"
                     /locus_tag="JNY02_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBB9432664.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29195..29608)
                     /gene="ibpA"
                     /locus_tag="JNY02_001042"
     CDS             complement(29195..29608)
                     /gene="ibpA"
                     /locus_tag="JNY02_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBB9432665.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29602..29674)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29913..30245
                     /locus_tag="JNY02_001043"
     CDS             29913..30245
                     /locus_tag="JNY02_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBB9432666.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30247..31384)
                     /locus_tag="JNY02_001044"
                     /pseudo
     CDS             complement(30247..31384)
                     /locus_tag="JNY02_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            31485..32503
                     /locus_tag="JNY02_001045"
                     /pseudo
     CDS             31485..32503
                     /locus_tag="JNY02_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32464..33801)
                     /locus_tag="JNY02_001046"
     CDS             complement(32464..33801)
                     /locus_tag="JNY02_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9432667.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33876..35024)
                     /gene="dgoD"
                     /locus_tag="JNY02_001047"
                     /pseudo
     CDS             complement(33876..35024)
                     /gene="dgoD"
                     /locus_tag="JNY02_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35021..35638)
                     /gene="dgoA"
                     /locus_tag="JNY02_001048"
     CDS             complement(35021..35638)
                     /gene="dgoA"
                     /locus_tag="JNY02_001048"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBB9432668.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35622..36489)
                     /gene="dgoK"
                     /locus_tag="JNY02_001049"
                     /pseudo
     CDS             complement(35622..36489)
                     /gene="dgoK"
                     /locus_tag="JNY02_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            complement(36486..37175)
                     /gene="dgoR"
                     /locus_tag="JNY02_001050"
     CDS             complement(36486..37175)
                     /gene="dgoR"
                     /locus_tag="JNY02_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBB9432669.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37453..38109
                     /locus_tag="JNY02_001051"
     CDS             37453..38109
                     /locus_tag="JNY02_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432670.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38155..38967)
                     /gene="yidA"
                     /locus_tag="JNY02_001052"
     CDS             complement(38155..38967)
                     /gene="yidA"
                     /locus_tag="JNY02_001052"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBB9432671.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMGNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39081..39479)
                     /locus_tag="JNY02_001053"
     CDS             complement(39081..39479)
                     /locus_tag="JNY02_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBB9432672.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39719..42133)
                     /gene="gyrB"
                     /locus_tag="JNY02_001054"
     CDS             complement(39719..42133)
                     /gene="gyrB"
                     /locus_tag="JNY02_001054"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBB9432673.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42162..43235)
                     /gene="recF"
                     /locus_tag="JNY02_001055"
     CDS             complement(42162..43235)
                     /gene="recF"
                     /locus_tag="JNY02_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBB9432674.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43235..44335)
                     /gene="dnaN"
                     /locus_tag="JNY02_001056"
     CDS             complement(43235..44335)
                     /gene="dnaN"
                     /locus_tag="JNY02_001056"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBB9432675.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44340..45743)
                     /gene="dnaA"
                     /locus_tag="JNY02_001057"
     CDS             complement(44340..45743)
                     /gene="dnaA"
                     /locus_tag="JNY02_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBB9432676.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46040..46120
                     /locus_tag="JNY02_001058"
     CDS             46040..46120
                     /locus_tag="JNY02_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432677.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46350..46490
                     /gene="rpmH"
                     /locus_tag="JNY02_001059"
     CDS             46350..46490
                     /gene="rpmH"
                     /locus_tag="JNY02_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBB9432678.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46507..46866
                     /gene="rnpA"
                     /locus_tag="JNY02_001060"
     CDS             46507..46866
                     /gene="rnpA"
                     /locus_tag="JNY02_001060"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBB9432679.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            46830..47087
                     /gene="yidD"
                     /locus_tag="JNY02_001061"
     CDS             46830..47087
                     /gene="yidD"
                     /locus_tag="JNY02_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBB9432680.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47090..48736
                     /gene="yidC"
                     /locus_tag="JNY02_001062"
     CDS             47090..48736
                     /gene="yidC"
                     /locus_tag="JNY02_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBB9432681.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48842..50206
                     /gene="mnmE"
                     /locus_tag="JNY02_001063"
     CDS             48842..50206
                     /gene="mnmE"
                     /locus_tag="JNY02_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBB9432682.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            50448..50522
                     /gene="tnaC"
                     /locus_tag="JNY02_001064"
     CDS             50448..50522
                     /gene="tnaC"
                     /locus_tag="JNY02_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBB9432683.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            50743..52158
                     /gene="tnaA"
                     /locus_tag="JNY02_001065"
     CDS             50743..52158
                     /gene="tnaA"
                     /locus_tag="JNY02_001065"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBB9432684.1"
                     /translation="MENFKHFPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            52249..53496
                     /gene="tnaB"
                     /locus_tag="JNY02_001066"
     CDS             52249..53496
                     /gene="tnaB"
                     /locus_tag="JNY02_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBB9432685.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWSAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGARLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            53628..54803
                     /gene="mdtL"
                     /locus_tag="JNY02_001067"
     CDS             53628..54803
                     /gene="mdtL"
                     /locus_tag="JNY02_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBB9432686.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            54778..>55423
                     /gene="yidZ"
                     /locus_tag="JNY02_001068"
     CDS             54778..>55423
                     /gene="yidZ"
                     /locus_tag="JNY02_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBB9432687.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT        13605 a        14753 c        13939 g        13126 t
ORIGIN      
        1 aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca ttgctatcgt
       61 gagtggtatg tgtttttacg ccagcgtact catggcgacg actccggcgg ttgagctgga
      121 attgcaaatt ctaaatgcca tcttccttgg tattctctgt ggtatcggca tgctttattt
      181 tcaggacttg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa atacttcacg
      241 cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct ggagctacca
      301 tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc tgctgtttat
      361 taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aacagagcca
      421 tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga
      481 aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga
      541 agttcagggg tctgtgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg
      601 actatgacgc gtttgatatt cgccggtttg attctgctga cgctgtcatt tgttcatggc
      661 gataaaatct tttctattat taacaatcac aaagatgcca ttagtctgct gattttttcc
      721 gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca
      781 gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg gttctcactg
      841 gtacgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc gctggtcggg
      901 acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta
      961 ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc ctcaacgctg
     1021 attgcccgct atggcacgtt gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt
     1081 ctattgcctt tttatgccag acaagggaca aactttgtcg ttaaaggcag tttgattttg
     1141 gcgttttttt atttgatcgt cattggtacg tccctgacat ttagtctgta cctgaaagga
     1201 gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc attaagtagc
     1261 gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg gctgggaacg
     1321 ctgctgattc tgtcatcggt aattttgatt tcaatggatt cccgtcgccg cgccagaaaa
     1381 ataaatcgtc cggcgcggga tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa
     1441 gatggtttct gccgctttag cgacttcgga tgattgataa gattgcagaa attccttcac
     1501 gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg gcgaattttt
     1561 atcttcaata aatacactgt cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt
     1621 gctgataatc gccacatcaa ctttgggatc atccagtacg cgcgggagct gcgccccctc
     1681 gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg taggtaataa
     1741 cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa gcgcacgacc
     1801 aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt gcgccacggt
     1861 tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca ccgctaccag
     1921 tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc
     1981 atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag
     2041 ttgaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg cgacatcttg
     2101 ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac tctggtcgca
     2161 acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat gatgtgttgt
     2221 cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta aggtcgctgc
     2281 ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt
     2341 ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga gcgatgaaag
     2401 caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc atgaaaccaa
     2461 cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac gccgagtcgc
     2521 cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat ttacgtaaaa
     2581 tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg
     2641 aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca
     2701 attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag caacaactac
     2761 tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg cagtaaccaa
     2821 caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca gaccaatatt
     2881 atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa taaccgccac
     2941 ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc acgttgacca
     3001 gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg tagcaacgat
     3061 tttatcgctt ccccacaacg tcagtaccaa cgcactcaca gccagtatta acggcgcgcc
     3121 tgctaaggcc agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa
     3181 gctggcgata ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag
     3241 gttcatatac accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa
     3301 gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt
     3361 ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag
     3421 cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa
     3481 actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag
     3541 cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca
     3601 gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt
     3661 agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta
     3721 gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa
     3781 ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat
     3841 tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc
     3901 caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc
     3961 aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa
     4021 cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga
     4081 gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac
     4141 ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg
     4201 aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg
     4261 catttcaggt tcggtttcac gcagaaaacc cagcgccagc ctgacctgag catcggtaat
     4321 cggcgcggaa aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct
     4381 atcatcgaca atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga
     4441 ctcatccggg ggatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc
     4501 gaccatcgac tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc
     4561 ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc
     4621 gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc
     4681 tcgccagtta ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca
     4741 cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa
     4801 agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa
     4861 acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca
     4921 gcacgccaac aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg
     4981 gtgagaagaa cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc
     5041 gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga
     5101 taaatgcgcc tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga
     5161 atttaccgtt gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg
     5221 aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg
     5281 cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc
     5341 cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca
     5401 gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac
     5461 ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga
     5521 caataacacc agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga
     5581 taccaatacg taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg
     5641 tgagcaaaaa tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga
     5701 tccccatcgc ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca
     5761 gcaccacggg taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac
     5821 aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca
     5881 caaaaacaat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga
     5941 cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca
     6001 cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct
     6061 tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta
     6121 atagatattc tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata
     6181 aaatcaaaat gagaaaaata tgaataattc cattaaccat aaatttcatc acattagccg
     6241 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
     6301 tgataatgtc tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat
     6361 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg
     6421 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
     6481 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
     6541 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
     6601 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc
     6661 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
     6721 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattaa
     6781 cgggcagaat gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca
     6841 ctgtccgggt ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
     6901 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
     6961 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
     7021 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
     7081 agggcatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
     7141 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
     7201 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     7261 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc
     7321 ggcgagactg gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc
     7381 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat
     7441 ccataacgaa ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     7501 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     7561 tggtttgctc ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga
     7621 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     7681 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct
     7741 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     7801 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     7861 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     7921 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg
     7981 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     8041 tctcgcggcg gattttgcgt tcttccatta ccgctactat cgccatcagg cagatacaac
     8101 caatcgcggc gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     8161 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     8221 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     8281 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     8341 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     8401 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc
     8461 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     8521 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     8581 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     8641 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     8701 cgctgacctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     8761 cgctaccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     8821 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg
     8881 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     8941 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     9001 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     9061 tggcactgaa gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt
     9121 tggtgttttt gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg
     9181 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     9241 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     9301 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     9361 ggtccagggt cggcttgcga acctggttta ggaaagccag catgggttac tcctgaaatg
     9421 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     9481 caagaaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     9541 tcacgcttca cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     9601 ggcggcgata gagataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     9661 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     9721 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg
     9781 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     9841 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     9901 cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat
     9961 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
    10021 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
    10081 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
    10141 ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg
    10201 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag
    10261 aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
    10321 atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
    10381 gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg
    10441 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
    10501 cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc
    10561 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
    10621 cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
    10681 gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag
    10741 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
    10801 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
    10861 catcgaacct tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
    10921 ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
    10981 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa
    11041 ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat
    11101 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc
    11161 tgattcatcg atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
    11221 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa
    11281 ccgtcctaac aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg
    11341 cccgctctgc ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
    11401 acgaatagca gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg
    11461 cacgctctct tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt
    11521 ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt
    11581 cagactttgc accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact
    11641 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg
    11701 ccaggccagc gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc
    11761 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
    11821 ccgcccgcgc cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
    11881 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa
    11941 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
    12001 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg
    12061 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
    12121 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
    12181 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
    12241 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag
    12301 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
    12361 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
    12421 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
    12481 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
    12541 cgtttggtca gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
    12601 taacagccgc ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
    12661 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
    12721 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
    12781 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
    12841 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
    12901 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
    12961 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
    13021 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
    13081 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
    13141 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
    13201 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
    13261 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt
    13321 tttgcatggc ttattccccc accatttcag tattcgccgc acctagcggc accatcgggt
    13381 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatta
    13441 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
    13501 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
    13561 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
    13621 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
    13681 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
    13741 tcgccacagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
    13801 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
    13861 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
    13921 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
    13981 gccactctgc acgcagttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
    14041 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
    14101 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
    14161 caaaacgcgc accgagcaca atcaacaaat ccgcctcctg caaaatatag ttggtactgc
    14221 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
    14281 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
    14341 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
    14401 ttgtagctgc gtcacgaatg ctttcttcgc taaaggaggg tgcagcggct ttttctgcca
    14461 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
    14521 acaccgggct agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga
    14581 gctcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
    14641 tgtccacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
    14701 aaatcagcgg gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac
    14761 ccggcccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
    14821 cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc
    14881 ttaaggcgtc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc
    14941 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg
    15001 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
    15061 ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca
    15121 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
    15181 gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt
    15241 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
    15301 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
    15361 ccgccggagc gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta
    15421 tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
    15481 cgtatttagt gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt
    15541 tatttataca gtaaacttct ataatatcac tgttcgcaat gtgttatgcg ggggccgcat
    15601 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
    15661 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
    15721 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
    15781 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
    15841 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
    15901 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc
    15961 tgacgcatct tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac
    16021 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    16081 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    16141 aaaagcatcc gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt
    16201 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    16261 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    16321 ttacggtgtc tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt
    16381 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
    16441 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    16501 aatggcgcgt actttgccgc gtgatttata taaacggaca cagttgcgcc atgctaacag
    16561 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    16621 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    16681 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    16741 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    16801 gctgattggc ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg
    16861 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    16921 ggcatttttt ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg
    16981 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    17041 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    17101 gctgtttccg ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    17161 tggtgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    17221 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    17281 gatcgtgctg tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta
    17341 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    17401 tcacgcatca cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    17461 agcacgctgt ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    17521 tggcagtcac ggctcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    17581 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt
    17641 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    17701 aattcaagat ggggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    17761 aaatcggcag gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca
    17821 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    17881 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    17941 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    18001 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
    18061 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    18121 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc
    18181 gtctggcttt acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc
    18241 atcaatatta agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    18301 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    18361 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    18421 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgccagaaaa
    18481 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    18541 attgttgctc ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt
    18601 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    18661 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    18721 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    18781 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    18841 ttaccactgt ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta
    18901 tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc
    18961 gctggggcat tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga
    19021 tattcctgat tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc
    19081 ccgccgggtg atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt
    19141 cactccttgc gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg
    19201 tgggcgtggg cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag
    19261 actccattgg taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc
    19321 atgcattttg ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt
    19381 tgggtcatta cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa
    19441 gtcatccgtc acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc
    19501 gtagcggtta aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc
    19561 cggcagaatc tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat
    19621 atgactgact ggcgtatcga cctgtcgccg ctccccttgc ggcgaacgga tgatcagcgg
    19681 aatacgggtg atgtcgtcat acatcgccgc tcctttactg atcagcttat gtgcgcccat
    19741 catttcaccg tgatcggagg tataaatgac ccacgtattt tcacgttgct ctggcgttaa
    19801 ggcattgatg acacgtccga tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg
    19861 atggtgataa agcccgtcat caccgactgg cgatagcatc gcctgcgccc ataagcggtg
    19921 atgttccggt ttgttcgcca ggtcatcctg tgatttctcg ccaagttcgt agtaaaaatc
    19981 agcgtatttc tctaaatact ccaccggaca ggtgaacggg tgattcggct catcatacga
    20041 aaccaccatc aggaagggtt cctcggcgcg cacgggctgt tgcagaaaat ctaccgctcg
    20101 attgctgatg cggtgcgccc aggtgaaggt ttcgtcaata tggttcgcct gtaaatcttc
    20161 gacgctgttt aggccattgc gccacaggct gatctctttt tccgtcagtt cgctaagata
    20221 gttcgcccca tcaaaccagt aatcagcatc ccactccggc ggacactcgc cagtgccgaa
    20281 atagtcatga ccgtcgagat gccatttgcc gatgtaacag gtgtgatagc cggcatcctt
    20341 aaagtagcgc cccatagtgg agatgttttt gcctggcgcg acgttgttgg tccacgggcc
    20401 ggactggtta gcgtagatac cggtgaatag tccggcgcgt gcaggcgtac aaaccggtga
    20461 acaagtgtag gcggaattaa agcgaatacc ttccgccgcc agactatcaa tattttgcgt
    20521 attcagcggt ttaccgctat agcaaccgac catattggtg gcctgggtat cggtcaattg
    20581 gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat
    20641 gccgccagct gtcaaaaatc aggtagacaa tcactacggc agcgatgaaa taactgatca
    20701 tcgctaacca gcgcgtaccg tagccgccga attcagccaa cccgacatag acgccaatca
    20761 tcgcgaacag gataccaatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg
    20821 caaacgcatc tttgaaggtg aacggcgtgg cgcgcgcttt gataaaaccg atcaccagca
    20881 tcacgaccac gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact
    20941 tcaccagata gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg
    21001 ccagcgccgg gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt
    21061 tgtaaatgcc gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc
    21121 acggcgctac caaaaccgaa acgatagcga taaagaaacc aaatttgcgc ccgacggtga
    21181 ccagctgctg cggctcggca ttctggttaa tgatgcgacg gtaaataccc atactgaata
    21241 atgtactggc gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg
    21301 cgccgaagaa acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca
    21361 tgtctgcttt cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta
    21421 ccagcgggtc gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca
    21481 ggctttttga cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt
    21541 tcaccagaat taaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga
    21601 ctgagtttaa tttctcggcg tgaacggtgg taatttgctc aatgccctgc ataaagctgc
    21661 ccttgcccat cgcgatcagg ccaaataccg gcaccatcaa cccgccgata accagcccaa
    21721 taccgttgat ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa
    21781 ttcccgccag accaagcaaa attaccagta gccagatagc cgcaccgtga gaaatctgta
    21841 gcgattcccc gacgtgaaac aggctgttga gcgccaacgc gccggagtag agaacaatcg
    21901 gcagaaagca gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt
    21961 tatcataacg ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg
    22021 gtaaaaagat tagcgcgagg aagatcagcg tcaccgctga agtcacttcc cagcccatca
    22081 ccgacatgcc gcttttgtag gcctgcccgg aaagaccaac cagttgttcc gtggaaaggt
    22141 tggttaacat taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat
    22201 agccttgttg cgatccggta tctgttttgc gaaccttcca ctaggtgatc accgccacca
    22261 gcagcgtaaa accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta
    22321 tgtttatgtc ccggacgcat gaatataggg agaggtaaaa tgtgtcgggt caggcagtca
    22381 gattcttaaa atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg
    22441 tgagcataat tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcagatgtaa
    22501 aaaacgaaac tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa
    22561 tttctctgat ctcgctctgg catacctacg ccgacgagca ttaccgggtg atctggccgc
    22621 gggacaagaa gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat
    22681 gtgggaaaat tcttttaaag aatggcgaac aaataactct gcatggcaac tgtattatat
    22741 ttttaaagcc aatggatatt cactcttatc actgtgaagg tttagtcttg gaacagtact
    22801 ggatggaatt tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt
    22861 ataatggcga aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag
    22921 aagcaataaa aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt
    22981 gtcttatgtc cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg
    23041 ccactttaca tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc
    23101 cctgtagcga agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag
    23161 aatattacct cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact
    23221 cggttggcga agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct
    23281 ttaaacataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
    23341 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
    23401 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg
    23461 cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
    23521 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
    23581 caggccgtag tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat
    23641 ccggctttca ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag
    23701 acgacgcgtg gcttctgcga caattgctgc cgggttggag tagttgagca tctaggcgtt
    23761 tggtgaatat ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc
    23821 gtacgctatt ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat
    23881 tttttcatcc agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc
    23941 aacgtcgctg aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt
    24001 ttctttaagg atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc
    24061 ataaaatttc agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat
    24121 gcctggcgta aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact
    24181 gcctccgtaa tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt
    24241 tcacgcaatt gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag
    24301 acgccgtgcg ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc
    24361 agtgcaatac gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc
    24421 agggcttgca ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt
    24481 tggcctcgcg aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt
    24541 cccggcgtgc acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg
    24601 gtaaagcaca acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc
    24661 gggatccagt tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca
    24721 ccaaagagat acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc
    24781 aacggtgaaa tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg
    24841 gttaaggttg cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg
    24901 aagtacatcg ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca
    24961 aaaccgcctt ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga
    25021 tgctgcgccc agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt
    25081 ccgtagatga agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc
    25141 cacaccccaa gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt
    25201 acttttggcc agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata
    25261 atcaccacat aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac
    25321 agacggttat gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta
    25381 tcgagggttt taatcccggc catcattgtc agaccactac ctgccaccgc gtcctgagtg
    25441 aaatcgacgc cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag
    25501 gtcaggaaac tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca
    25561 atgggtaaac cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct
    25621 tcaataatgt gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca
    25681 aacatcgggt tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc
    25741 agcactggcg tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc
    25801 tcttttctca attctgtaag taggaagcct gattggttat gcggcagacc tggagcgagg
    25861 ataggaagat gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc
    25921 ttttcgttgg gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg
    25981 tacacagcat cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt
    26041 gtgggcgcat tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca
    26101 ttgcggagcg gttaagaatt cgacttaatt ccgcggattt cacgctaaac ggccttcttc
    26161 ccggtgaaaa aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca
    26221 ttgacctgct ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg
    26281 tgcgcaaaga tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac
    26341 ggcagggaaa aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg
    26401 ccattgccag ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc
    26461 gttttgtgga agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc
    26521 gtaacctttc actgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg
    26581 ggattttgat tggcggtaat tatgaaagcc tgatgccggt gctcgccgat agactgctgg
    26641 cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata
    26701 gcgagagcgg ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg
    26761 tggagtacca tttacggcga ccccacccgg aaaagagtta accgatactc cagaagagca
    26821 ccgccagtaa ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt
    26881 aagagagcgc cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag
    26941 gatcggtcat ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca
    27001 tccgcgccag aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa
    27061 tccagcttag cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac
    27121 caacgaccga gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg
    27181 gcataaacca gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca
    27241 gcggaccgcc cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa
    27301 tagaacctaa caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt
    27361 gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc
    27421 ccaccaggtt gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga
    27481 ccccggcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc
    27541 gaatacgttt tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag
    27601 tgcctttcgt agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta
    27661 aatccgctgg ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg
    27721 aaggtacttt tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga
    27781 gaatcggtac atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga
    27841 tagtcttgat cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct
    27901 cgacattgac gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc
    27961 cgaatggata cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac
    28021 ccaggtcgcg caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg
    28081 agaaaatccc cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa
    28141 ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc
    28201 gcaatgaggc aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca
    28261 ggccaaattc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa
    28321 caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc
    28381 gaaatttgac gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg
    28441 ttaatgctat atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt
    28501 atggctgaat cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg
    28561 ccgaaatatc ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc
    28621 cccaccagaa tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc
    28681 tgatagcgat acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca
    28741 gtaaaccgtt tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca
    28801 ggctaaatgg ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct
    28861 ccggcgtgcc ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac
    28921 ggaaacctgc cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt
    28981 acggcgggaa gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac
    29041 cgatccattg acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta
    29101 agaagcgagt aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg
    29161 gagatgtcag gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt
    29221 ttttcgcttc cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag
    29281 ccccacgaac atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga
    29341 tgccctggta cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca
    29401 gcagattatc ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag
    29461 caatgcggta atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac
    29521 tctggctctg gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt
    29581 aaagcgggga taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta
    29641 agaccttcca caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga
    29701 ctacgatgta aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt
    29761 gcagtttatg gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc
    29821 cgccacagtg cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag
    29881 gccaacaccc acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca
    29941 tgatatgcag cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg
    30001 ggaaagaagg aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa
    30061 ccaactgggg cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca
    30121 cgcttttact gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa
    30181 aaagcgaagc caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata
    30241 actgatttag cgatcggttt cggtgatcca cagctggcca ccttccatcc acgccagcca
    30301 ttgaccatcc ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt
    30361 taaatactca acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa
    30421 gcccaaccat tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg
    30481 ggttaacttg cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt
    30541 atcatcacgc attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg
    30601 cgggtagggt tgtttccgtt ccacttaacg gcgcatcacc tgccgatttc cagctagctt
    30661 catcttgcgg taactcaacg ataaatagct ccggcacttt ctcgcccttt ggcgaaagtg
    30721 tgtcgccaat aaacgccagc gcgtgatttc ctacccatcc ttcttcataa gcacgattga
    30781 tttcatcact gccaggctgc ggcgtgggcg tggttttact caccagcaca caccagtggc
    30841 taccgctgta ttcacgcgga tgttgctttt gtacgttgac cgggccaaac ggtgcagcaa
    30901 ccccgacgtt tcgcaaatcc agcgccggat cgagttgatg cattacatgg tcgttatagg
    30961 taaagctccc cctttcaccg ttcgggctaa agacatgcac atggctgcca ccgcgcagcg
    31021 ctcctggcgt atacggcgcg gtaatatcca ttgcatcgag attgctcacc ttgccgcctt
    31081 cagcaatgac tccgcgtcga tgatggaagt cgtaatgcca tgtttcatca ggattttcag
    31141 ggccgtgaat aaagacatat ttctctgact tcggatgaac ggtcaccacg ccgacgtgtg
    31201 cgccctgtga cgcgcgatag ataacctcga cctcgcctgt atggatattc acacgctcaa
    31261 tggtctcgcc ggtaaacgac gccccagaag gacgcacgtc aaataccagc cactggctgt
    31321 cgggcgtcca ggtattggta ttggtgagca ggtgattacg gggagcaaag gttatctgtt
    31381 tcatggcgcg gtcctgatac aaaacggttc ccgccatcat acttcacaag gacttcactt
    31441 tcagacaata gtcttttgcc atcgtgatga atgtgagtga aaagatggaa catttcgacg
    31501 tggcgattat tggcctcggc ccggcagggt cggcgttggc acgaaagtta gccggcaaaa
    31561 tgcaggtgat cgcgctggat aaaaagcacc agtgcggtac tgaaggtttc agcaaacctt
    31621 gcagattttc agcgtcaaaa ctgtcgacgt cgccgcatcg ctcacgcgta actaccagcg
    31681 aagctatatc aatattaatc gccatgcttt cgacttgtgg atgaaatcgc tgatccccgc
    31741 cagcgttgag gtttatcacg atagcctgtg ccgaaaaatc tggcgtgagg atgataaatg
    31801 gcatgtcatt tttcgtgcag acggctggga gcaacatatt accgcccgct atctggtcgg
    31861 tgccgatggc gcaaactcga tggtgcggcg acatctctac ccggatcatc aaatccgtaa
    31921 atatgtcgct atccagcagt ggttcgcaga gaaacatccg gtgccgttct actcctgcat
    31981 ctttgataat gcgataactg actgttactc atggagtatc agcaaagatg gttattttat
    32041 ctttggcggt gcctatccaa tgaaagacgg tcagacgcgt ttcacgacgc tgaaagagaa
    32101 aatgagcgcc tttcagttcc agtttggcaa agcggtgaaa agtgagaaat gcacggtgtt
    32161 gtttccctca cgctggcagg attttgtctg tggtaaggac aacgcctttc tgattggtga
    32221 agcggcggga tttatcagcg ccagctcgct ggaggggatt agctatgcgc tggatagcgc
    32281 agagattctg cgtgcggtgt tcctgaaaca gccggagaag agcaacgccg cttactggcg
    32341 cgccacacgc aaactgcgtt taaaactctt cggcaagata gtaaaaagcc gatgcctgac
    32401 cgcaccggct ttaagaaagt ggattatgcg cagtggtgtg gcgcatattc cacagttgaa
    32461 agattatcca acgcgcttca catcacccac cagcaggatg taagagagcg cgccaatcaa
    32521 cgcgacggcg gagatataaa ccagtgcagg cgcgaaaccg taaccctgcg ccaggtaccc
    32581 caccaccagc ggaacggtga tgccgcccag accaccggcg aagttaaaca cgccgccggt
    32641 taaaccaatc aggcgcatcg gtgccagaga agagaccagc gaccaggtaa tcgaagcaaa
    32701 accgttgccg aagaatgcca gcgccatcag gcacataatc atcatcggat cgttggtgta
    32761 gttagcgccc ataatgcagg tggagatcag caagccgcag ataatcggcg ttttacgcgc
    32821 aaagcccagt gaaaagccct tacgtaccag cagatccgcg acccagccag agagcaggac
    32881 gccgacaaac gccgcgagga atggcaccgt ggtcataaag cctgctttca gcgccgtgat
    32941 tcctttttcc tgggttaaat agttcgggaa ccaggttaag aaaaaccaca gtgcagaagc
    33001 caccgcaaat tgcccaagat aaacgccgat cagtttacga tggaacacca gtttccagtc
    33061 tttggctgtt aacggctgac gcgcctcttt cttcaccggc gcatcaccat ccaccagacc
    33121 gccgccatca cgaatgtaat ccagttcagc tttgctgata cctttggtca ggcgcggcgg
    33181 ctgataaacc ttaaaccaaa tcagcgacca gataatgccg ataccaccag tgacaatgaa
    33241 cacccagtgc cagctcaaca tctcctgaat ccagatcagc agcggagtca aaaacgccag
    33301 accgacaaac tgaccagacg tataaaaacc aacggcggaa gcgcgttcat gttccggaaa
    33361 ccagctggtc accatccggt tattggtcgg gaaagcgggc gcttcgaaaa taccggttat
    33421 cgcacgcaga ccaattaatg acattaatcc cgtggcaaag ccctggaata aagtcgccac
    33481 tgaccaacca aatatcgcaa taaaataagt cacgcgagaa cctacgcgat ctaaaaacca
    33541 accgccgggg atctggcata gcgtataaag ccaggcgaag gccgaaaata catagcccat
    33601 ttccgctttc gtaatgccga actcttcctg aatatgggcg gaagccacgg ccaggttagc
    33661 gcggtcaaca taacaaatga ccaccgtaat aaagatcatc accagcgtca gataacgccg
    33721 acgccccggc tttgctgcat taacgggaat atccatagcg agctttctcc agattttggg
    33781 catagcgaag ccgctcacca tgccctgtaa tttacagagg gttatatttt tgtattgctg
    33841 ttttagtgcc cgatgagggg cttacgtggc aggaattacc actctgctac gctgttatct
    33901 tcatgacgcc agagcggatt acgccagtcc ggggcatttt tactgaactc aataacttta
    33961 gcttcgtcga tttccacacc taagcccggt ttcgttaacg gtttaaagaa gccgccaacc
    34021 atgctgaagt cttctttgtt tttcacaaag tcgagtaact ccgcgccttt gttgtaatga
    34081 atgcccatac tttgttactg aagtaccgcg ttataggaaa caaagtcgat atgcaggcaa
    34141 gccgccagtg caatcggtcc gaacggacag tgcggcgcaa gggtcacatc ataggcttct
    34201 gccattccag caattttgta gcattcggta ataccgcctg catgggagag atccggttgc
    34261 agaatcgaaa taccgcctgc ctccagcacg cgtttaaaat cgaaacgtga gaacatgcgc
    34321 tcacccgccg ccagtggaat atgcgtttgt gccgccaatt tcgggtagta ttcggcttgt
    34381 tccgccagca ccggctcttc aataaacagc gggcgatacg gctccagttc tttaatcagc
    34441 actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa gaccaaactc aatctgattg
    34501 ccaaaagctt cacgaatttg tgccacggtg ttgactgccg catctaccgc gcgggagtta
    34561 tcaattagcc ccagttcttc acaaccgttc agtttgaagg tatcgaagcc gatttcgcgc
    34621 agggttttaa tgccgtcgat aacatccgcc ggacgatcgc cgccgaccca actgtaggct
    34681 ttaattttgt cgcgaaccag gccgcccatc agttgccaga ctggcgcatt cagcactttg
    34741 cctttgatat cccataacgc ctggtcgatc ccggcgatgg cgctcatcag gattggaccg
    34801 ccacgataaa atccggcgcg atacatcact tgccataagt cgttgatgcg cgaaggatcc
    34861 tgaccaatca aatagtcacc cagctcgtga actgccgctt ccaccgtacg ggcgcggcct
    34921 tcaatcacgg gctcgcccca accgaccacg ccttcatcgg tttcaatttt caggaacatc
    34981 cagcggggag gtaaacgata cgtggtaatt ttggtgattt tcattgcact gcctctcgat
    35041 acgccttaac aaatgctgct gcctgctgcg cggtgcgttc tacggactgc ccggcgcgat
    35101 aaagatcgct gcctaagccc gccccaacac aacctgcgtc tatccactgc gccaggtttt
    35161 ctggcgtcac gccgccaacg gcaaagactg cgatgtccgg tggtaatacc gcttttaacg
    35221 ctttgatgta ttgcggacca aaagccgatg acggaaatat tttcagcgcc tgcgcgcccg
    35281 cttcgagcgc ggtaaaggct tcggtcgccg tcgcgcagcc ggggcaaacg gtcatgccgt
    35341 agcccaccgc gcggcggatc acttcactat ggatattggg cgtaacgatg agctgacagc
    35401 ccatcctggc gagcgcatcg acctgttcag gtttcagtac cgtacctgcg ccaatcaacg
    35461 ccttgtcgcc atacgcatca acgatggcgg gaatgctctg ctcccattgt ggggaattca
    35521 gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc gccaacatgc gccagcgcct
    35581 cgtcgggcgt aatgccgcgc aaaatggcga tcagcgggag tttagtttgc cactgcatga
    35641 gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg ccaccgccgc cacgtcgcaa
    35701 cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg atgttccggc gacaagggtg
    35761 atggcgtgtt gatgggtcac atagtcgcgc atactggcaa cctctgcgcc aatcaacaaa
    35821 ccagagagaa tgttcgcgga gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag
    35881 ctgcggcaat atggcgggcg cattaaggcc gcgctcaagg ccagccgcga aggcatcggc
    35941 agagttttcc tgcggcggca aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg
    36001 taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca
    36061 tttgcaatgc gtaccgggca taacataaag agaggaagga gccagagctc gcgcgccgat
    36121 caattgtgtt tcttcgccgc gcatcacatt gtggttatcg tcatgagaga cgcataatcc
    36181 gggaataatc cagatattgt cgccaacaga cgttaattgt tcgccaatag acgaaaaacg
    36241 ggcaggaaca gataaatacg gagcaacttt ccagccgaca ttgctgccga ccattcctgc
    36301 catcaccact ggcgtatttt cttcacgcca gtcggtcgtg acttctgcta acaccgcagc
    36361 cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg
    36421 gtcgccctga taaagccagg cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg
    36481 agctgtcatg tgatttcctt taaccttcgt gtcgagctgg caatcatggt aagcgccgcc
    36541 tgctctgccg catcgccgtc ctgatgccgt atcgcatcga acagcgcctt atgttcctgg
    36601 agcgtttgcg gcatgttggc ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc
    36661 agcgaactga tcgcaatgct aagttgctgt aacaccgggt tatgcaccga ctgcagcacc
    36721 gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc
    36781 atctcgttca gcgccgattc aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc
    36841 caacgggcaa tcgccggttc caccagattt cgcacttcgc tcatggcact gataagccgt
    36901 gggtcgtagt cgttttccag cacccattgc agtacgtcag tgtcgaggta attccactgg
    36961 ttacgcggtg ccacaaacgc cccgcgataa cgtttcattt caatcagccg cttcgccatc
    37021 agcgaacgga acacctcacg gatgatgttg cgcgaggttg caaactcttc acagagctcc
    37081 gcctcagccg gaagtggcga gccaggtacg tatttgccgt gaacgatctg tttacccagc
    37141 gtaatgacaa tgcgatcggt tttattgaga gtcatggaga gtccttgtgc tctgtatgtt
    37201 cttctctact ttaccccgat cgatgcataa cgcggcaact ttgtagtacc agcgtgatga
    37261 cgttcgcgtt tgccgtgcgt gtaatgtagt acaaacttat attgttgtgc tacaatttag
    37321 atcaaaaaaa gaacaatgca taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga
    37381 tatcaggcgc taaacaggag ggaaagaaga gtatgctttc aacggcttag ctactcgttt
    37441 aaaagattaa tcatgaagtt gaattttaag ggatttttta aggctgccgg tttattccca
    37501 ctggcgctga tgctttcagg ctgtatctcg tatgctctgg tttcccatac cgcaaagggt
    37561 agttcaggaa agtatcaatc gcagtcagac accatcactg ggctatcgca ggcaaaagat
    37621 agtaatggaa caaaaggcta tgtttttgta ggggaatcgc tggattacct tatcactgat
    37681 ggtgccgatg acatcgttaa gatgctcaat gatccagcac ttaaccggca caatattcag
    37741 gttgccgatg acgcaagatt tgttttaaat gcggggaaaa agaaatttac cggcacaata
    37801 tcgctttact actactggaa taacgaagaa gaaaaggcac tggcaacgca ttatggtttt
    37861 gcctgtggtg ttcaacactg taccaggtca ctggaaaacc taaaaggcac aatccatgag
    37921 aaaaataaaa acatggatta ctcaaaggtg atggcgttct accatccatt taaagtgcga
    37981 ttttatgaat actattcacc cagaggcatt ccggatggtg tttccgcagc attactgcca
    38041 gtgactgtta cgctggacat cattactgca ccgctgcaat ttctggttgt atatgcagta
    38101 aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt
    38161 cagcacatac ttctcaatag caaacgccac gccatcttca aggttggatt ttgtgacaaa
    38221 gttcgccatt tctttcaccg aaggaatagc gttacccatc gccacaccga cgcctgcata
    38281 ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc atgatttctt ccggtttaat
    38341 acctaacacg tcggccagtg atttcacacc cgtacctttg ttaacgcgtt tatcgaggat
    38401 ttcgaggaag tacggcgcac ttttcagcac ggtatatttc tctttcactt cctgcggaat
    38461 acgcgcgata gcctggtcga ggatggcggg ttcatcaatc atcatcactt tcaggaactg
    38521 ggtattgggg tccattttct ccgcttcgca gaacaccagc ggaattgtgg caacgaagga
    38581 ttcatgcacc gtgtagtagc tgatatcgcg gttagcggtg tacagcgtgg tgcggtccag
    38641 ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc aggaaacgat agtcgtcata
    38701 gctgagagcg gtttgcgcca cggtgctacc atcagcggcc ttctgtacca gcgcgccgtt
    38761 ataagtaatg cagtagtcgc ccggctgttc catatgcagc tctttcaggt agttgtgcac
    38821 acctgcatac ggacgacccg tcgttagcac gacattcacg ccacgggcgc gagctgcggc
    38881 aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc agcagaaggg tgccatccat
    38941 atcgatagca atgagtttga tagccatgag ttcccaaggt agattagttc ctgacccatg
    39001 ctaacgcgat tccgctcaaa aatcagtaca acacccgagg gaaaagggga tgcaacgcgg
    39061 gttgcatgct ccccatttgc ttagcggaag agtttccctt tcagcagttc catgcctgcg
    39121 gaaagcagat cgttattggc ttgtgctgac acttcacctt gcggtgagag cgcatcaata
    39181 atcttcggca attgctctgc cagtaaactg gaagccgtac tggtatccac gccaagtttt
    39241 tgcccgagat cggacaccgc atttgtgccg agcgccgatt ccagttgctc gccgctaact
    39301 gattgatttc tctgttgatt actcaaccaa gttgagagaa tggcccctaa gccgccactt
    39361 tgcagttttt ccagcagcac ctgaatgcca ccctgctcct caacccaact taaaatcgcc
    39421 tgatatttcc ccgcatcgcc tttcagaaag gcaccgacaa cttcatcaaa aagccccatg
    39481 ataatcacct gtaaagcgtt acgtgttgac ccaaaaagta tagatctgcg ggatgataat
    39541 tgcggattgc agaaataaaa agggcggaga tgatctccgc ccttttctta tagcttcttg
    39601 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatca tgcaaaatca atatattgca
    39661 ggagtctcgt aggactaata agcgtagcgc atcaggcacg ctcgcatggt taacgccatt
    39721 aaatatcaat attcgccgct ttcagggcgt tctcttcaat aaacgcacga cgcggttcta
    39781 cggcgtcgcc catcagcgtg gtgaacaact ggtcagcggc aatcgcatcc ttaacggtaa
    39841 cgcgcagcat acgacggctt tccgggtcca tggtggtttc ccacagctgt tccgggttca
    39901 tctcgcccag acctttataa cgctggatgg agaggccacg acgggactct ttcaccagcc
    39961 agtccagcgc ctgctcgaag ctggctaccg gctgacgacg ctcgccacgt tcgataaacg
    40021 catcttcttc cagcaagcca cgcagtttct cacccagcgt gcagatacga cgatattcgc
    40081 caccggtgat aaactcgtga tccagcggat agtcagtatc cacaccgtgg gtacgcacgc
    40141 gaacaatcgg ctcgaacagg ttttgttcgg cattggtgtg gacatcaaac ttccactggc
    40201 tgccgtgctg ttctttgtcg ttcagttcgc tgaccagcgc gttcacccag cgggtaacgg
    40261 tctgctcatc agaaaggtca gcttccgtca acgtcggctg atagataagc tctttcagca
    40321 ttgctttcgg ataacgacgc tccatgcggt tgatcatttt ctgcgtcgcg ttgtactcag
    40381 ataccagttt ctctaacgct tcgccagcca atgccggtgc actggcgttg gtgtgcagcg
    40441 ttgcgccgtc cagcgcgata gagatctggt actgatccat cgcttcgtcg tctttaatgt
    40501 actgttcctg cttgcctttc ttcactttgt acagcggcgg ctgagcgatg tagacgtgac
    40561 cgcgttcaac gatttccggc atctgacgat agaagaaggt caacagcagc gtacgaatgt
    40621 gcgagccgtc gacgtccgca tcggtcatga tgatgatgct gtgataacgc agtttgtccg
    40681 ggttgtactc gtcacgaccg ataccacagc caagcgcggt gataagcgtc gccacttcct
    40741 gagaagagag catcttatcg aagcgcgctt tctcgacgtt aaggatttta cccttcagcg
    40801 gcagaatcgc ctggttcttg cggttacgcc cctgcttcgc agagccgccc gcggagtccc
    40861 cttccaccag gtacagttcg gaaagcgccg gatcgcgttc ctggcagtct gccagtttgc
    40921 ccggcaggcc agctaagtcg agcgcacctt tacggcgggt catttcacgc gcgcgacgcg
    40981 ccgcttcacg ggcacgggca gcatcgataa ttttgccaac cacgattttc gcatcagttg
    41041 ggttttccag caggtattcc gccagcagtt cgttcatctg ctgttcaacc gccgatttca
    41101 cctcagaaga aaccagtttg tctttggtct gggaggagaa tttcgggtcc ggcactttta
    41161 cggaaacgac cgcaatcagg ccttcacgcg catcgtcacc ggtggcgctg actttggctt
    41221 ttttgctgta gccttctttg tccatgtagg cattcagggt acgggtcatc gccgcacgga
    41281 agcctgccag gtgagtaccg ccgtcacgct gcggaatgtt gttggtaaag cagtagatgt
    41341 tttcctggaa gccatcgttc cactgcagcg ccacttcaac gccaataccg tctttttcgg
    41401 tggagaagta gaagatattt gggtggatcg gcgttttgtt cttgttcaga tattcaacga
    41461 acgctttgat gccgccttca tagtggaagt ggtcttcttt gccgtcacgc ttgtcgcgca
    41521 gacgaatgga aacgccggag ttgaggaacg acaactcacg cagacgtttc gccagaattt
    41581 catattcgaa ctcggtcaca ttggtgaagg tttcgaggct gggccagaaa cgcaccatgg
    41641 tgccggtttt ttcagtctcg ccggtaaccg ccagcggagc ctgcggtaca ccgtgttcgt
    41701 agatctgacg gtgaatttta ccctcgcgct gaataaccag ctccagtttt tgcgacaggg
    41761 cgtttactac cgaaacacca acgccgtgca gaccgccgga cactttatag gagttatcgt
    41821 caaatttacc gcctgcgtgc agaacggtca tgatcacttc cgccgccgat acgccctctt
    41881 ccgggtgaat accggtcgga atgccgcgcc cgtcatcctg tacagagaca gagttatcgg
    41941 cgtgaatggt gacgataatt tctttacagt gacccgcgag cgcttcgtcg atagcgttat
    42001 ctaccacctc gaataccatg tggtgcagac cggtgccgtc atccgtgtcg ccgatataca
    42061 tacccgggcg cttacgcacc gcatccagcc ctttcaggac tttgatactg gaggagtcat
    42121 aagaattcga catcaacgtt tctcgctcat ttatacttgg gttaatccgt tattttaccc
    42181 ttttccacgg taaacatctt cgaattttcg tccgacatgt ctataacgtg ttcagcactg
    42241 atcgcgctga caaagacctg tgattgcgtc gcttttaagc ggctggcaag caacccgcga
    42301 cgctcatcat caagctcaga ggcaaaatca tctatcaggt agagacaccg ccgcccgctt
    42361 tcacgagtga ggaactctcc ttgcgccaga cgtaaggcgc acatcaacag cttaagctgc
    42421 ccacgcgata aggtatcttc caccggcgca ccgtcggcgc gaatgcgtaa gtccgcttta
    42481 tgcgggccat gcgcggtgta ggttagctgg cgatcgcgtt caaaattacg ttccagcacc
    42541 tcagcgtact ctgtctcttt ctcccagccg cgctggaaag agaaagtcag agaaaactca
    42601 gggagaaatt gcttacaggt atcatccata tcggccgcga taccggcgct atactccgcg
    42661 cgccaggtgc tgatttgctc cgccagcggg atcagttctt tatcccacgg gcgtagctgt
    42721 tcgtaacgag tcacctggcg cagcgccgca ttgcgctgct tgagcaatcg cttgagattg
    42781 ctccaggcgg tgaaaaatcc aggttcgttg tgaaagcatc cccagtcgag gaatgctctt
    42841 ctgtatttgg ggccgccgtt gagtaaagta aacccttctg gcgttatcaa ctgcatcggc
    42901 atcaggtgcg ccagttccgc gactttatgc ccgtccgtac cgtcaatacg gactttgctg
    42961 tcgccctgtt tgtctttggt taagccaatc gccgtctcgc gctcttcgcc ctgtaatcgc
    43021 ccgtggagaa caaatgcctc ctgctcatgg cgaatgacgc gaccaatctg caaactgcga
    43081 aacgcccgac catggccgag cgtatagatg gcttccagca cgctggtttt gccactgccg
    43141 ttggcaccta ccagaaagtt aaagccggga gataaagcga gatccgcggt ttcaatattg
    43201 cggaaatcgc ggatcaacaa gcgggtgagg gacattacag tctcattggc atgacaacat
    43261 aggcagccga ctgtgatgcg gcatcttcaa tctgcacgct ggaaaccgaa tcggtcagca
    43321 tcatgcggac gttttcgcat ttcagcgcgt tcagaacatc cagcacatag ctgacgttga
    43381 agccgatttc catctccgca ccgctatagg taacgtcgag gatctcttcc gcttcttcct
    43441 gttccgggtt gttggcagta attttcagct ggttttcgct gacatacagg cgcacaccgc
    43501 ggaatttctc gttagagaga atcgccgcgc gagcaaacgc ctgcttgagc agatcgcagc
    43561 ctgcttccag atgtttgtcc gggttcttcg gcagaacgcg acggtaatcc gggaagcgac
    43621 catccaccag tttcgaggtg aaaataaagt cgccaacgtg ggcacgaatg ttattgctgc
    43681 caatctgcac acgcagcaga ttgtcgccgc cgtcgagcat acgcatcagt tcaatcacgc
    43741 ctttacgcgg tacgatcacc gaatggcttg gcaaagattg accaattggc attgaacaga
    43801 ccgccagacg gtggccgtcg gttgccacgg tgcgcagttc ttcaccttcg gtttcaaaca
    43861 gcataccgtt taagtaatag cgaacatcct gatgcgccat cgaaaactgg gttgcttcaa
    43921 tcagacgctt catcgtcgcc tgcggcaggg taaattcgac ttcgctctgc cagtcatcga
    43981 ggttcgggaa atccgccgct ggcagggtag acagcgaaaa acggctacgc ccggagcgca
    44041 ccagcatccg ttcaccttcc agctgcacgg caatttccgc gccttcaggc agaccacggc
    44101 agatatcaaa gaatttgcgc gccggaacgg tcgtcgctcc tggctcgtgt ggctgaacca
    44161 gcgcaacgcg tgccaccatt tccatctcga gatcagtacc ggtcagcgac aacgtaccgt
    44221 cagcaacctg taacagcaga ttaccgagaa tcggtagcgt aggacgacca cctaacggac
    44281 cgctcacctg ttgtagcggt tttaataaat gctcacgttc tacggtaaat ttcataggtt
    44341 tacgatgaca atgttctgat taaatttgaa aaatcttctt tgatatcgtg gctctcttca
    44401 cgcaactgct cgatcttacg gcaggcatga agcaccgtcg tgtggtcacg cccaccaaac
    44461 gcatcgccaa tctctggcag actgtggtta gtcagctctt tcgctagcgc catcgccatc
    44521 tggcgcggac gcgccaccga gcgggatcga cgcttggaaa ggagatccgc gactttgatc
    44581 ttgtagtact ccgccaccgt cttctgaata ttgtcgatgg tgaccagttt ttcctgcaat
    44641 gccagcaagt cgcgcagcgc ctcacgcacg aagtcgatgg tgatcgcccg tccggtaaag
    44701 ttggcgttag caataacgcg gttcagcgcc ccttccagct cacgtacgtt agatcgtaga
    44761 cgcttggcga taaagaacgc cacttcaccc ggcaaacgaa tgtcattttc gtcggccttt
    44821 ttcatcagga tcgccacacg ggtttccagc tctggcggtt cgatcgccac agtcagtccc
    44881 caaccgaagc gggatttcaa acgatcctca acgccgttga tctctttcgg atagcgatcc
    44941 gaggtgagaa tgatctgttg attaccttcc agcagggcgt tgaaggtgtg gaaaaactct
    45001 tcctgagatc gttctttatt agcaaaaaac tgaatatcgt cgatcagcag tgcatctacg
    45061 gaacggtagt agcgtttaaa ctcttcgatc gcgttgtttt gcagggcttt aaccatgtcc
    45121 tgaacaaagc gctcggagtg catataaacc actttggcat tcggcttgcg cgccataatg
    45181 ccgttaccca ccgcatgcag caggtgagtt ttacccagac ccgtgccgcc ataaaggaac
    45241 aacgggttat aggcaccgcc agggttatcc gccacctggc gagccgccgc gcgcgccagt
    45301 tggttagatt taccttcaac gaagttatca aacgtgtgtt tgacgtttac gttagaacga
    45361 taggtcggtt ctgccggggc cgggacgtta tcccaacctg agcgcgtaga aggcgcagca
    45421 cgttgcggct gcgtttgcgc cacctgtgca ggggccgcga cgttgctcgt cactgccgct
    45481 tgtggcgttt gcgtcaccgg tttggtgccg acttcaaaac gcagctgtgg ggcatccgct
    45541 ccgcagaaac tggttagcag tccattgata ttattaaggt acttgtcccg tacccaatcg
    45601 aggacaaaac ggtttggcgc gtacagggac agcgtgttat cgctcagttc cgcctgcaat
    45661 gggcgtatcc acatactgaa ttctgtggct ggtaactcat cctgcaatcg ggcaagacac
    45721 tgctgccaaa gcgaaagtga cacggcggac tccactcgaa caaaagtcga taatgactaa
    45781 ggctgaaaca ttcatgattg ttgacgtacg tcgaaaagac cctgcttgag ggtgacgcac
    45841 gaaccgctgt ctgcggttat atgcccgatc aagatcctgc aaaacgatcg ggaccgcgga
    45901 tcatagccta aactgcgcaa gagatcttct gtttctcaca gattttccct atttatccac
    45961 aggactttcc agaactcgct aagtgtaaac gatcctgccg cgaggcgggc acgatttacg
    46021 ccgcatattg gaaaatttaa tgaccataga caaaaattgg cttaatcgat ctaataaaga
    46081 tccaggacga tccttgcgct ttacccatca gcccgtataa tcctccaccc ggcgcgccat
    46141 gctggtttcc actggtgtga ggtcgtacat tttccctgcg aaaaggtgcg gaaaagcgcg
    46201 gtaaataagg aaagagaatt gactccggtg tgtacaatta ttacaatccg gcctctttaa
    46261 tcacccatgg cttcggtgtc catcgtttca tttttcggcg gatatccaat aaagccattg
    46321 aatttattca agtttaggta gaaatcgcca tgaaacgcac ttttcaaccg tctgtactga
    46381 agcgcaaccg ttctcacggc ttccgtgctc gtatggctac taaaaatggt cgtcaggttc
    46441 tggcacgtcg tcgtgctaaa ggccgcgctc gtctgaccgt ttctaagtaa taaagctaac
    46501 ccctgagtgg ttaagctcgc atttcccagg gagttacgct tgttaactcc cagtcaattc
    46561 acattcgtct tccagcagcc acaacgggct ggcacgccgc aaattaccat tctcggccgc
    46621 ctgaattcgc tggggcatcc ccgtatcggt cttacagtcg ccaagaaaaa cgttcgacgc
    46681 gcccatgaac gcaatcggat taaacgtctg acgcgtgaaa gcttccgtct gcgccaacat
    46741 gaactcccgg ctatggattt cgtggtggtg gcgaaaaaag gggttgccga cctcgataac
    46801 cgtgctctct cggaagcgtt ggaaaaatta tggcgccgcc actgtcgcct ggctcgcggg
    46861 tcctgatagc cctcattcgg gtctatcaac gcctgattag tccgctactc gggccgcatt
    46921 gtcgtttcac tccaacctgt tcaagctacg gaattgaggc attgcgcagg tttggagtga
    46981 taaaaggcag ttggttgacg gtgaaacgcg tattaaaatg ccacccttta caccctggtg
    47041 gtgacgatcc cgtcccgccc ggaccatttg ataccagaga acactaacga tggattcgca
    47101 acgcaatctt ttagtcatcg ctttgctgtt cgtgtctttc atgatctggc aagcctggga
    47161 gcaggataaa aacccgcaac ctcaggccca acagaccacg cagacaacga ccaccgcagc
    47221 gggtagcgcc gccgaccagg gcgtaccggc cagtggccag gggaaactga tctcggttaa
    47281 gaccgacgtg cttgatctga ccatcaacac ccgtggtggt gatgttgagc aagctctgct
    47341 gcctgcttac ccgaaagagc tgaactctac ccagccgttc cagctgttgg aaacttcacc
    47401 gcagtttatt tatcaggcac agagcggtct gaccggtcgt gatggcccgg ataacccggc
    47461 taacggcccg cgtccgctgt ataacgttga aaaagacgct tatgtgctgg ctgaaggtca
    47521 aaacgaactg caggtgccga tgacgtatac cgacgcggca ggcaacacgt ttaccaaaac
    47581 gtttgtcctg aaacgtggtg attacgctgt caacgtcaac tacaacgtgc agaacgctgg
    47641 cgagaaaccg ctggaaatct cctcgtttgg tcagttgaag caatccatca ctctgccacc
    47701 gcatctcgat accggaagca gcaacttcgc actgcacacc ttccgtggcg cggcgtactc
    47761 cacgcctgac gagaagtatg agaaatacaa gttcgatacc attgccgata acgaaaacct
    47821 gaacatctct tcgaaaggtg gttgggtggc gatgctgcaa cagtatttcg cgacggcgtg
    47881 gatcccgcat aacgacggta ccaacaactt ctataccgct aatctgggta acggcatcgt
    47941 cgctatcggc tataaatctc agccggtact ggttcagcct ggtcagactg gcgcgatgaa
    48001 cagcaccctg tgggttggcc cggaaatcca ggacaaaatg gcagctgttg ctccgcacct
    48061 ggatctgacc gttgattacg gttggttgtg gttcatctct cagccgctgt tcaaactgct
    48121 gaaatggatc catagctttg tgggtaactg gggcttctcc attatcatca tcacctttat
    48181 cgttcgtggc atcatgtacc cgctgaccaa agcgcagtac acctccatgg cgaagatgcg
    48241 tatgctgcag ccgaagattc aggcaatgcg tgagcgtctg ggcgatgaca aacagcgtat
    48301 cagtcaggaa atgatggcgc tgtacaaagc tgagaaggtt aacccgctgg gcggctgctt
    48361 cccgctgctg atccagatgc caatcttcct ggcgttgtac tacatgctga tgggttccgt
    48421 tgaactgcgt caggcaccgt ttgcactgtg gatccacgac ctgtcggcac aggacccgta
    48481 ctacatcctg ccgatcctga tgggcgtaac gatgttcttc attcagaaga tgtcgccgac
    48541 cactgtgacc gacccgatgc agcagaagat catgaccttt atgccggtca tcttcaccgt
    48601 gttcttcctg tggttcccgt caggtctggt actgtactat atcgtcagca acctggtaac
    48661 cattattcag cagcagctga tttaccgtgg tctggaaaaa cgtggcctgc atagccgcga
    48721 gaagaaaaaa tcctgattcg gtgagttttc gctaaaataa gggcggtcag ttgaccgcct
    48781 tttttctttt cgtagggcgg ataagcaccg cgcatccgcc acacaaagca acaggaacat
    48841 catgagcgat aatgacacta tcgtagccca ggccacacct ccgggacgtg gcggcgttgg
    48901 catcctgcgc atctccggct tcaaagcccg tgaagttgcc gaaaccgtgc tgggtaaact
    48961 gcctaagccg cgctacgccg attatcttcc gtttaaagac gccgacggca gcgtgctcga
    49021 tcaggggatt gcgctatggt tccctggccc gaactcgttc accggcgaag atgtgctgga
    49081 actgcaaggt catggcggtc cggtgatcct cgacctgctg ttaaaacgca ttctgaccat
    49141 tcccggcctg cggattgctc gccctggtga gttttccgaa cgcgcgtttc ttaacgataa
    49201 acttgactta gcccaggccg aggcgattgc cgatcttatc gacgccagtt cggaacaggc
    49261 ggcccgttcg gcacttaact cgctgcaagg cgcattctcc gcacgggtta atcatctggt
    49321 agaagccctc acccacttgc gcatttacgt cgaagcggca attgatttcc ctgatgaaga
    49381 gatcgatttc ctctccgacg gcaaaattga agcccagctt aatgacgtga tggccgatct
    49441 cgatgcagtg cgtgctgaag cacgtcaggg tagtttgttg cgcgaaggga tgaaagtagt
    49501 gattgccgga cgtcctaatg ccggtaaatc gagcctgtta aacgcgctgg cgggccgtga
    49561 agcggcaatc gtaaccgata tcgccggaac cacgcgtgac gtgctgcgtg agcatatcca
    49621 cattgacgga atgccgctgc atatcatcga cactgccggg ctacgtgagg ccagtgacga
    49681 agtggaacgt attggtatcg agcgcgcgtg gcaggaaatt gaacaggccg accgcgtgct
    49741 gtttatggtc gatggcacca cgacagacgc cgttgatccg gcagagatct ggccggaatt
    49801 tatcgcccgt ctgcccgcga aactgccgat caccgtggta cgcaataaag ccgatatcac
    49861 cggcgaaacg ctgggaatga gtgaagtgaa cggtcacgcg ttaattcgtc tctcggcaag
    49921 gactggtgaa ggcgtggacg tgctgcgtaa ccatctcaaa cagagcatgg gctttgacac
    49981 caatatggaa ggcggcttcc tggcgcgccg tcgccactta caggcgctgg aacaggcagc
    50041 ggagcatttg cagcaaggta aagcgcaact gttgggagcc tgggcaggtg aactgctggc
    50101 ggaagagttg cgcctggcac agcagaactt aagcgaaatc accggggaat ttacttcaga
    50161 cgacctgctg gggcggattt tctccagctt ctgtattggt aagtaaccgc gcttacgaag
    50221 ccgtattctg actgtcagat gcggcttcgc ttcattgtta gcactcctgt tatgcctcaa
    50281 ccctttttta aacattaaaa ttcttacgta atttataatc tttaaaaaaa gcatttaata
    50341 ctgctccccg aacgattgtg attcgattca catttaaaca atttcagaat agacaaaaat
    50401 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
    50461 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
    50521 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
    50581 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
    50641 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
    50701 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
    50761 ttccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
    50821 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
    50881 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
    50941 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
    51001 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
    51061 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
    51121 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
    51181 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
    51241 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
    51301 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
    51361 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
    51421 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
    51481 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
    51541 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
    51601 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
    51661 tatggcggcc tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
    51721 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
    51781 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
    51841 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
    51901 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
    51961 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
    52021 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
    52081 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
    52141 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
    52201 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
    52261 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
    52321 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gagtgccttt
    52381 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
    52441 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
    52501 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
    52561 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
    52621 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
    52681 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
    52741 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
    52801 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
    52861 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
    52921 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
    52981 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
    53041 ggcaacgttg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
    53101 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
    53161 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
    53221 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
    53281 tttatttacg ggatcggcgg tgccaggctg tgcgccacca tctgggcggt cattattccc
    53341 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
    53401 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
    53461 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
    53521 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
    53581 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
    53641 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
    53701 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
    53761 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
    53821 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
    53881 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
    53941 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
    54001 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
    54061 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
    54121 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
    54181 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
    54241 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
    54301 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
    54361 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
    54421 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
    54481 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
    54541 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
    54601 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
    54661 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
    54721 gcattgtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
    54781 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
    54841 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
    54901 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
    54961 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
    55021 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
    55081 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
    55141 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
    55201 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
    55261 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
    55321 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
    55381 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cct
//
LOCUS       DADWTH010000018        55110 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.018, whole genome shotgun sequence.
ACCESSION   DADWTH010000018 DADWTH010000000
VERSION     DADWTH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.018"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..354
                     /locus_tag="JNY02_001069"
     CDS             <1..354
                     /locus_tag="JNY02_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBB9432688.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="JNY02_001070"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="JNY02_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBB9432689.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="JNY02_001071"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="JNY02_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBB9432690.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="JNY02_001072"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="JNY02_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBB9432691.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="JNY02_001073"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="JNY02_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBB9432692.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="JNY02_001074"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="JNY02_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBB9432693.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRATD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="JNY02_001075"
     CDS             6877..7281
                     /locus_tag="JNY02_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBB9432694.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="JNY02_001076"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="JNY02_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBB9432695.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="JNY02_001077"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="JNY02_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBB9432696.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="JNY02_001078"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="JNY02_001078"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBB9432697.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="JNY02_001079"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="JNY02_001079"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBB9432698.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12182
                     /locus_tag="JNY02_001080"
                     /pseudo
     CDS             10704..12182
                     /locus_tag="JNY02_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12252..13028
                     /gene="yaaA"
                     /locus_tag="JNY02_001081"
     CDS             12252..13028
                     /gene="yaaA"
                     /locus_tag="JNY02_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBB9432699.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13181..13477)
                     /locus_tag="JNY02_001082"
     CDS             complement(13181..13477)
                     /locus_tag="JNY02_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBB9432700.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13691..14977)
                     /gene="thrC"
                     /locus_tag="JNY02_001083"
     CDS             complement(13691..14977)
                     /gene="thrC"
                     /locus_tag="JNY02_001083"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBB9432701.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14978..15910)
                     /gene="thrB"
                     /locus_tag="JNY02_001084"
     CDS             complement(14978..15910)
                     /gene="thrB"
                     /locus_tag="JNY02_001084"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBB9432702.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15912..18374)
                     /gene="thrA"
                     /locus_tag="JNY02_001085"
     CDS             complement(15912..18374)
                     /gene="thrA"
                     /locus_tag="JNY02_001085"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBB9432703.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18400..18519)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18455..18520)
                     /gene="thrL"
                     /locus_tag="JNY02_001086"
     CDS             complement(18455..18520)
                     /gene="thrL"
                     /locus_tag="JNY02_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBB9432704.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18734..19420)
                     /locus_tag="JNY02_001087"
     CDS             complement(18734..19420)
                     /locus_tag="JNY02_001087"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBB9432705.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19820..19960)
                     /locus_tag="JNY02_001088"
     CDS             complement(19820..19960)
                     /locus_tag="JNY02_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432706.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20056..20772
                     /gene="arcA"
                     /locus_tag="JNY02_001089"
     CDS             20056..20772
                     /gene="arcA"
                     /locus_tag="JNY02_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBB9432707.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20831..22183)
                     /gene="creD"
                     /locus_tag="JNY02_001090"
     CDS             complement(20831..22183)
                     /gene="creD"
                     /locus_tag="JNY02_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBB9432708.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22241..23665)
                     /gene="creC"
                     /locus_tag="JNY02_001091"
     CDS             complement(22241..23665)
                     /gene="creC"
                     /locus_tag="JNY02_001091"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBB9432709.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23665..24354)
                     /gene="creB"
                     /locus_tag="JNY02_001092"
     CDS             complement(23665..24354)
                     /gene="creB"
                     /locus_tag="JNY02_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBB9432710.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGVDDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24367..24840)
                     /gene="creA"
                     /locus_tag="JNY02_001093"
     CDS             complement(24367..24840)
                     /gene="creA"
                     /locus_tag="JNY02_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBB9432711.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25051..25920
                     /gene="robA"
                     /locus_tag="JNY02_001094"
     CDS             25051..25920
                     /gene="robA"
                     /locus_tag="JNY02_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBB9432712.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25917..26564)
                     /gene="gpmB"
                     /locus_tag="JNY02_001095"
     CDS             complement(25917..26564)
                     /gene="gpmB"
                     /locus_tag="JNY02_001095"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBB9432713.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26616..27137
                     /gene="yjjX"
                     /locus_tag="JNY02_001096"
     CDS             26616..27137
                     /gene="yjjX"
                     /locus_tag="JNY02_001096"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBB9432714.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27222..27548)
                     /gene="trpR"
                     /locus_tag="JNY02_001097"
     CDS             complement(27222..27548)
                     /gene="trpR"
                     /locus_tag="JNY02_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBB9432715.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27638..29575)
                     /gene="sltY"
                     /locus_tag="JNY02_001098"
     CDS             complement(27638..29575)
                     /gene="sltY"
                     /locus_tag="JNY02_001098"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBB9432716.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29786..31453
                     /gene="ettA"
                     /locus_tag="JNY02_001099"
     CDS             29786..31453
                     /gene="ettA"
                     /locus_tag="JNY02_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBB9432717.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLDQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGSQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31560..31727)
                     /locus_tag="JNY02_001100"
     CDS             complement(31560..31727)
                     /locus_tag="JNY02_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432718.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31761..32993)
                     /gene="nadR"
                     /locus_tag="JNY02_001101"
     CDS             complement(31761..32993)
                     /gene="nadR"
                     /locus_tag="JNY02_001101"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBB9432719.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(33014..34396)
                     /gene="radA"
                     /locus_tag="JNY02_001102"
     CDS             complement(33014..34396)
                     /gene="radA"
                     /locus_tag="JNY02_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBB9432720.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34445..35413)
                     /gene="serB"
                     /locus_tag="JNY02_001103"
     CDS             complement(34445..35413)
                     /gene="serB"
                     /locus_tag="JNY02_001103"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBB9432721.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVLANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35519..36163
                     /locus_tag="JNY02_001104"
     CDS             35519..36163
                     /locus_tag="JNY02_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBB9432722.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTQLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36191..37207
                     /gene="lplA"
                     /locus_tag="JNY02_001105"
                     /pseudo
     CDS             36191..37207
                     /gene="lplA"
                     /locus_tag="JNY02_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37239..37502
                     /locus_tag="JNY02_001106"
     CDS             37239..37502
                     /locus_tag="JNY02_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9432723.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37663..38382)
                     /gene="deoD"
                     /locus_tag="JNY02_001107"
     CDS             complement(37663..38382)
                     /gene="deoD"
                     /locus_tag="JNY02_001107"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBB9432724.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38439..39662)
                     /gene="deoB"
                     /locus_tag="JNY02_001108"
     CDS             complement(38439..39662)
                     /gene="deoB"
                     /locus_tag="JNY02_001108"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBB9432725.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39714..41036)
                     /gene="deoA"
                     /locus_tag="JNY02_001109"
     CDS             complement(39714..41036)
                     /gene="deoA"
                     /locus_tag="JNY02_001109"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBB9432726.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDH
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41163..41942)
                     /gene="deoC"
                     /locus_tag="JNY02_001110"
     CDS             complement(41163..41942)
                     /gene="deoC"
                     /locus_tag="JNY02_001110"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBB9432727.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRYMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42200..43741
                     /locus_tag="JNY02_001111"
     CDS             42200..43741
                     /locus_tag="JNY02_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBB9432728.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSL"
     gene            43713..44576
                     /locus_tag="JNY02_001112"
     CDS             43713..44576
                     /locus_tag="JNY02_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBB9432729.1"
                     /translation="MNSRCALVCKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44790..45572)
                     /locus_tag="JNY02_001113"
     CDS             complement(44790..45572)
                     /locus_tag="JNY02_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBB9432730.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45569..46642)
                     /locus_tag="JNY02_001114"
     CDS             complement(45569..46642)
                     /locus_tag="JNY02_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBB9432731.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46764..46925)
                     /locus_tag="JNY02_001115"
     CDS             complement(46764..46925)
                     /locus_tag="JNY02_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBB9432732.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47052..47657)
                     /gene="osmY"
                     /locus_tag="JNY02_001116"
     CDS             complement(47052..47657)
                     /gene="osmY"
                     /locus_tag="JNY02_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBB9432733.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48050..49639)
                     /gene="prfC"
                     /locus_tag="JNY02_001117"
     CDS             complement(48050..49639)
                     /gene="prfC"
                     /locus_tag="JNY02_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBB9432734.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49730..50407)
                     /gene="yjjG"
                     /locus_tag="JNY02_001118"
     CDS             complement(49730..50407)
                     /gene="yjjG"
                     /locus_tag="JNY02_001118"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBB9432735.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50422..50868)
                     /gene="rimI"
                     /locus_tag="JNY02_001119"
     CDS             complement(50422..50868)
                     /gene="rimI"
                     /locus_tag="JNY02_001119"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBB9432736.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50837..51250)
                     /gene="holD"
                     /locus_tag="JNY02_001120"
     CDS             complement(50837..51250)
                     /gene="holD"
                     /locus_tag="JNY02_001120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBB9432737.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRVNPTARAALWQQICTYEHDFFPRND"
     gene            51353..52384
                     /gene="rsmC"
                     /locus_tag="JNY02_001121"
     CDS             51353..52384
                     /gene="rsmC"
                     /locus_tag="JNY02_001121"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBB9432738.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52583..52669
                     /locus_tag="JNY02_001122"
     tRNA            52583..52669
                     /locus_tag="JNY02_001122"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52617..52619,aa:Leu,seq:cag)
     gene            complement(52708..52944)
                     /locus_tag="JNY02_001123"
     CDS             complement(52708..52944)
                     /locus_tag="JNY02_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBB9432739.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53085..53873
                     /gene="fhuF"
                     /locus_tag="JNY02_001124"
     CDS             53085..53873
                     /gene="fhuF"
                     /locus_tag="JNY02_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBB9432740.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(53911..54588)
                     /gene="bglJ"
                     /locus_tag="JNY02_001125"
     CDS             complement(53911..54588)
                     /gene="bglJ"
                     /locus_tag="JNY02_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBB9432741.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(54546..>55110)
                     /locus_tag="JNY02_001126"
     CDS             complement(54546..>55110)
                     /locus_tag="JNY02_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB9432742.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13199 a        14864 c        13753 g        13294 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaaatc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaaac cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagaaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aaggcagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agtataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcta ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggtg
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcacc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ttctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tccacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggagctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagtcgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcattcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggagcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc acgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttacgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccgcc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg ttttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 cgggaatggg atcgacgcca aacgcggcag cggcagcggc agcggcagcg gcagcggcag
    11581 cggcagcggc agcggcagcg gcagctgcat cctggcctcc gcatccggca gcacaaggga
    11641 ttgtccagat gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc
    11701 tgatattact ggcgggtaac ggcacaacct acatgccgct ggaaggtatt cagcttatcc
    11761 agaaggcgat gcgggtgcta atgggttcct agggtgctga atttgttacc ctcgtggtta
    11821 ttctgtttgc cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct
    11881 ttttacgcct gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg
    11941 tcatcggcgg caccttgcta agccttccgc tgatgtggca actggcagat atcataatgg
    12001 cctgcatggc tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca
    12061 ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc
    12121 gttatccgga tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt
    12181 aatcacaact atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc
    12241 aaggactgga tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt
    12301 tgaccaccac gcgctatacg ctgccggagc tgttagacaa ttcccagcag ttgatccatg
    12361 aagcgcggaa actgacgcct ccgcagatta gcacgctgat gcgcatcagc gacaaactgg
    12421 cgggtatcaa cgccgctcgc tttcatgact ggcagccaga tttcacgccg gcgaatgccc
    12481 gccaggcgat tctggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaaccttca
    12541 gcgaagacga tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg
    12601 tactgcgccc gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga
    12661 atgcccgagg gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg
    12721 aggcgctcgc agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta
    12781 aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga
    12841 agaacggcaa atttaagatc atcagcttct acgctaagaa agcacgcggt ctgatgagtc
    12901 gtttcattat tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag
    12961 gttacttctt tgatgaagat tcctccagca atggcgaact ggtgtttaaa cgctacgagc
    13021 agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag
    13081 catgcagatt caatatattg cagaattaac gtaggcctga taagacgcgc cagcgtcgca
    13141 tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg
    13201 gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg
    13261 gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt
    13321 cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt
    13381 ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg accagaacca
    13441 gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc
    13501 cgttatgggt cgatatccgg agattacgcg atgagaacga cggttgttat tctccgcaat
    13561 cctaatctct aagttaccgc atttattgat aatttctcct ttttccctgt cgtttttctc
    13621 cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa
    13681 tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagaaaaa tcggcgggca
    13741 gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg
    13801 tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga
    13861 acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct acggcagcgt
    13921 gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat
    13981 catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt
    14041 ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag
    14101 tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg
    14161 tcgcagcaat aaaacgtttc accggcagac cgaatgactt cgccagcaga cccgccgtca
    14221 aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgcc tcctgcggca
    14281 gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatcg
    14341 agttagccga gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca
    14401 ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca
    14461 acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca
    14521 ctttcacatt cggcaaaccg tagaaagcat gagccactgc agctccggta tcaccggagg
    14581 tcgcggtcag aatggtcact ggcttatcat ccgcaatatg ggtcagcatt tgtgccataa
    14641 agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga
    14701 catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt
    14761 ccaggatttc ctgtgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg
    14821 tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcgt
    14881 gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga
    14941 cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc
    15001 ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac
    15061 ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg aacaaggtcg
    15121 ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct
    15181 gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct ttcatcagct
    15241 tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc
    15301 cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg
    15361 tcgagacttt aattcccgga tacgccagca cccacagcca ctcatcaaac cctggcactt
    15421 gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg
    15481 ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc cagctcgccc atcaaagcca
    15541 gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt catcgccatc agcgccgcga
    15601 ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg catattcttt tccagagtca
    15661 tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac tgataaacga
    15721 tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga
    15781 atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgcca
    15841 ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa
    15901 ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca
    15961 ccggcagcgg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga
    16021 taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca
    16081 ttcccatcca cttcggcaat cttcacgcgg cagacaccat cttcatcaat attgccaaca
    16141 tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg
    16201 agctgtgaca gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc
    16261 agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc acgagcgaga
    16321 atcaatagtt tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta
    16381 taacccattt cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg
    16441 ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcaccaa ttcatcacct
    16501 gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca
    16561 tagaggaatt tacgccgcga tttttccgcc gcataacgca actgatgata gtaatccatc
    16621 gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg
    16681 gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga
    16741 tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgtgca
    16801 agttcttcct gccagttttc cagattaagg ccatgcacac tagtgagcag agccttcgag
    16861 ttggcaacgc cgcagacacg taagtcgata tgtttattct tcagccagct ttgctgacgc
    16921 ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata
    16981 acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg
    17041 ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg
    17101 atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata
    17161 ccatcaccta caaccgagat aatggccagc cgttccgtca ccgccagcgg ctccagtaag
    17221 ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagctcg cacacagtcg
    17281 ctttgtggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg
    17341 gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg ccatgccaac catccctttc
    17401 atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc
    17461 ggtaattcgt cttcatcacg gctggcacca atgagcgtac ctggtgcttg aggatttccg
    17521 gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga
    17581 agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgacttcaac
    17641 aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac gtccgtccaa
    17701 atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg
    17761 ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg cggtgaaacc tgccatcaac
    17821 accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc agcaatatcg
    17881 acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc gataacggta
    17941 acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca
    18001 cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact aatgccatgc
    18061 aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg cgccagcggg
    18121 aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg
    18181 ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg
    18241 ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt
    18301 tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt accgccgaac
    18361 ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc ccgcactgtc
    18421 aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa
    18481 tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa
    18541 tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat
    18601 aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag acactctttt
    18661 tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt
    18721 actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca
    18781 ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg taaccagtcg accagtttta
    18841 tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca
    18901 gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat gttgctaatt
    18961 gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc gccaccatcg
    19021 gcacaccagt aagcacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc
    19081 gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac agcggcacca
    19141 gttctactgg cgtggcgtag taatgatatt tcgcccgact acgcgcagtg gtggcgacag
    19201 tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa aactttaata ttatcaataa
    19261 tatcactaga tccatgtgcg acccagcggg tggctggctc caggtgtgcc tgactatcga
    19321 caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc ccaatatttt
    19381 ctgctctggc gggtgcgacc aggataatcg ttatacgcat attgccactc ttcttgacca
    19441 aataaccgcg aacagggtga tcactgtcaa cttattacgc ggtgcgaatt tacaaattct
    19501 taacgtaagc cgcagaaaaa gccctttact tagcttaaaa aaggctaaac tattttctga
    19561 ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat
    19621 tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac
    19681 tagttggatt attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa
    19741 actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg
    19801 tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg
    19861 acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga
    19921 tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat
    19981 ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca
    20041 atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca
    20101 cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat
    20161 ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc
    20221 aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt
    20281 gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc
    20341 ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc
    20401 aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa
    20461 agctacaagt tcaatggttg ggaactggac atcaacagcc gttcgttgat cggccctgat
    20521 ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac
    20581 ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa
    20641 ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg
    20701 ccggatacgc cggaaatcat cgccaccatt cacggtgaag gttatcgctt ctgcggtgat
    20761 ctggaagatt aatcggcttt accaccgtca aaaaaacggc gctttttagc gccgttttta
    20821 tttttcaacc ttatttccag atacgtaatt catcgtccgt tgtaacttct ttactggctt
    20881 tcattttcgg cagtgaaaac gcataccagt cgatattacg agtcacaaac atcatgccgg
    20941 ccagcgccac caccagcaca ctggttccca acaacagcgc gctatcggca gagttgagca
    21001 gtccccacat cactccatcc agcaacaaca gcgcgagggt aaacaacatg ctattgcgcc
    21061 agcctttcaa taccgcctgc aaataaatac cgttcattaa cgccccaatc agactggcga
    21121 ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc cagcaagagc aaataaaaca
    21181 tcaccaatga aagccccacc agcaaatatt gcattgggtg taaacgttgc gcggtgagcg
    21241 tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag cagaatggcg tacttagtcg
    21301 cccggtccgt taattggtat tgatcggctg gcgtcgtcac tgcgacgcta aacgcgggga
    21361 agttttccca gccggtatca ttgcctgaag caaaacgctc accgagatta ttagcaaacc
    21421 agctgctttg ccattgcgcc tgaaaacctg actcgctagc ttcccgtttg gctggtagaa
    21481 aatcacctaa aaaactggga tgcggccagt tgctggttaa ggtcatttcg ctattacgcc
    21541 cggcaggcac cacagaaaga tcgccggtac cgcttaaatt cagtgccata ttcagctgca
    21601 ggttctgctt ccgccagtcc ccttcgggta aagggatatg cacgccctgc ccgccttgct
    21661 ctaacccggt accgggttca atggtcagtg ccgttccgtt aacttcaggc gctttcacca
    21721 caccaatacc acgcgcatcc ccgacgctaa tcacaataaa gggcttgcct aagatgatat
    21781 ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc ggcttttagc gttaaatcac
    21841 tgtgccagac ctgaccggta taaatcccta tcttgcgttc ttccacgttc tgattgccat
    21901 caaccatcaa tgactcaggt aaccaaaaat ggataaaact tcgtttccgc tccacggttt
    21961 tatcctcttc ctgcaccgta taaagctcgg tcacaggaat agcgataagc ggcccaacga
    22021 gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc cacatcgcta cggtaatcgg
    22081 cacgttcgac aatcacttgc cgaatcagca ttatcggaat caacaacagc aatactgcac
    22141 caaacaaggt ggtcattttc cagaacaggg gggatttcaa catagtcttc tcctttgcaa
    22201 tggcagcagg atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag ttacggtgaa
    22261 gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact tcgccgttaa
    22321 acaaacgggc gacctcactg acgaacgcca accccagacc gctgcttttt tgcccatttg
    22381 cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca ggaataccac
    22441 taccggtatc cagcacctta agcgtgacgt gttcctgatc cacttcggcg cttagcgtta
    22501 tgcaaccgct ctcgggagta aaatcgatgg tgttatccag taaattcccc agcgcctgat
    22561 ccagtaacgc cggttcagca gcaacattaa cctcggtggg cataacatgc aaagtgatgt
    22621 ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcgaaataat gccgccacat
    22681 caacagcagt cagaacgact tcctgacgat tctccagtct tgcctggcgt agtaacgttt
    22741 ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt gtcagtaaaa cgagccacta
    22801 cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc gccagtgggc
    22861 tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc ccttccagct
    22921 tcacgcgcat actttccagc gcctgcgcga gtttacgcag ctcgctacta ccgagatcgg
    22981 gaagaggaac gggcttattg tcagtgacgg aatcagcata gcgagtgagc cgggcaatgg
    23041 agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc aacaaaatgg
    23101 cgctggccca taaaattcgc cgctcgctgc gtttaatgac cggagccatc gccgcgttcg
    23161 gtttgcctac gctcaaaacg ccaataagcc gcgagccgcc cataatcggc gcggcaacat
    23221 acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca ccatactgac
    23281 cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgtccaacg gctttatttg
    23341 ccgaatcgaa caatactttg ccatgcgcat cggtcatata gacatgatat tcattgcgca
    23401 ctttgttaat gccaccaata ttggcgcgaa acgggcgatg ttgtagctga ttaaacgcct
    23461 gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc agctccgcaa
    23521 gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct tcgcacgccc ggtttaactt
    23581 ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa aaatagccca
    23641 gcagcaaccg catgccgata cgcattacaa gcccctcagg ctatatccca tgccgcgatg
    23701 agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca gcgttttaat
    23761 gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc tatccattag
    23821 ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca ataaaaactc
    23881 ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg gttcattcaa
    23941 ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag aacttcttca cccgacgcag
    24001 taaggtgcgc accctggcgc acacttcgcg gggtgaaaac ggtttagcca catagtcgtc
    24061 aacgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg tcaggaacag
    24121 tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc cactgatatc
    24181 aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc tggcgagctt tatccagcac
    24241 cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca tgtagaccag
    24301 cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt gcataaatcg
    24361 cctctgttat tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt tcggcgaacc
    24421 ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg cgtcataaaa
    24481 gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt tttttgaata ctacctcgcc
    24541 ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt gctgacaaga
    24601 aatggccgca tcggaggtat cttctgccag gcccaatccc cctttaatac caccggtttt
    24661 cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg cttccacaac
    24721 aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa tctcttctgc
    24781 atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct tgtatttcat
    24841 attgttacca tttttatcta ttacacgagc aattagttcg tcacggtaaa taacatattg
    24901 attaagaaca aaatctcaat tcttttattt ccgtcaggcc ctaaaacata ctctactaag
    24961 aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc tgatgtcagg
    25021 tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat tcgcgacctt
    25081 ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt agcggcgaaa
    25141 gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg ccatgctatt
    25201 ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg cctgactgcg
    25261 cgtccgattc tggacatcgc gctgcaatac cgcttcgact ctcaacagac atttacccgc
    25321 gcattcaaga agcagtttgc ccagactcct gcactttacc gccgttctcc tgaatggagc
    25381 gcctttggta ttcgcccgcc gctacgtctg ggtaaattca caatgccaga gcacaaattt
    25441 gtcaccctgg aagatacgcc gctgattggt gttacccaga gctactcctg ttcgctggag
    25501 caaatctctg atttccgcca tgaaatgcgt tatcagttct ggcacgattt tctcggcaac
    25561 gcgccgacca ttccgccggt gctctacggc ctgaatgaaa cgcgtccgag tcaggataaa
    25621 gacgacgaac aagaggtatt ctataccacc gcgttagccc aggatcaggc agatggctat
    25681 gtactgacgg ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt tacctatgaa
    25741 ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat acggaacgtg catgccaatg
    25801 ctcaatctga cgcgccgtaa aggtcaggat attgagcgat actacccggc agaagacgcc
    25861 aaagcgggag atcgcccaat taatctgcgc tgtgaactgc tgattcctat ccgccgttaa
    25921 cgctgcagct catctaatgc aggggcgtcc agatgcgaga tgtcccctgc ggtttcaacg
    25981 acccagccag acgccagcca caggctttcc tgataatcca cgcgcgaaat agaacagtta
    26041 cgcagacgta agcggcgctc tgcccatgct ggtaatccga gaatcgtact caccagacat
    26101 cccagtgcaa taccatggct caccagcagc ggtcggcttc cctgcggtaa gtcccggcaa
    26161 gattccagtg ccgcattgac gcgatcgctg agctcctgca ttgactcgcc ttcaggaata
    26221 cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt tctcttcttc ttcggtcaga
    26281 gaatcgatat gtcttttttc cagcacaccc atgtttaatt cacgcaggcg agaatcaaag
    26341 atgatgtcac agccgcaggc ctgggcgatg atttccgccg tacgccgggt gcgtcctaaa
    26401 tcgctactga taatatgcgt aatgccaagc tctttggcac gggttgccac ctgcatcgcc
    26461 tgttgctcac ctttggcggt cagcgggctg tcagactggc cctgaatacg tcgctcggcg
    26521 ttccactgcg tttcaccgtg gcggactagg tatacctgta acatgctttt tttccgtata
    26581 ctgctgtaac gttaattttc tgagtaatgc tgattatgca ccaagttgtc tgtgcgacca
    26641 ccaatcccgc taaaattcag gccattctgc aggcatttca cgagatcttc ggcgaaggat
    26701 cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc ggaacaaccc tttggcagtg
    26761 aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc ccgccgttta cttccagagg
    26821 ctgatttttg ggtggcgatt gaagctggca ttgatggcga cagcactttc agttgggtgg
    26881 tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc aactttgccg ttacctgcgg
    26941 tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc cgtgatgtcg cgttataccg
    27001 gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc gggaaactca
    27061 ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt cacaatgccg
    27121 tgtatcaggc attgtaggac ggataaggcg ttcacgccgc atccgacatt cggtgcacga
    27181 tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa atcaatcgct tttcagcaac
    27241 acctcttcca gccactgacg cagctccacg ggcgcggctt tcaggctgtt agatccacgc
    27301 gtaatcgtcg cgatgcccgc gccgagttca ttttttaact cacgctgact catttcgcca
    27361 cgcaacagct cttcgacaat acgcacgcga gtccccaacg cttcgcgctc atctggcgtc
    27421 agcatcaggt ttaacaacgg taaatggaga tcgttttggt aggcattctt aagcaggtcg
    27481 acaaaacgta accactcctg gtgacgctgt tctgccatcg ctgctgaata gggtgattgt
    27541 tgggccataa tatgtcgcca ttgttagcgg gggaagcaaa atgactcccc cggttgtact
    27601 cgctaaagag tacgatagca tatcataaac gtgcggatca gtaacgacgt ccccattccg
    27661 tggcgctcat caacgtcggt ttatccccca tgaaatagcg gtagtaagca tcataagcca
    27721 gcacgttctt cacataaccg cgcgtctcgg agaacggaat actctcgaca aatgccacag
    27781 cgtcgatacg cccggcgctg ttgccaagcc aggttcgcac ccgtccaggt ccggcgttat
    27841 aagctgctga ggagaaaata cggttattgc caaactgctg ataaacatat tgcaggtaac
    27901 tggtgccaat gttgatgttc gtttccggat ccagcaattg cccagggctg ctataaccag
    27961 gaatagagaa catcttcacc gtatgggtcg ctgtaccagg cataatctgc atcaggccgc
    28021 tggcccctac cggtgatttc actttcggat tccaggcgct ctcctgacgg gcaatcgcca
    28081 tcgcatagct ttgcgggatc tctttcccac tggtgtaacg tttgaaaaga tcgttgtaag
    28141 ccagcgggaa tcgctcttcc agatgatccc aaagcttccc ggcgatcgtt gcctgaacgc
    28201 taagatccca ccattggtta ttgaaagcat accgagccag ttgagcctgc tctgtttttg
    28261 acttgctctt caccagattg gcccactcgc tacgcgcggt attatcgaga ttccagtaca
    28321 tcaactcgcg cacgcgcgcc atctccggcc cctgagtcag ggtgctgtca acattctgcg
    28381 gcgctttatc aatcttcagc tcatactctt caccgatgcg ttgtgctgca accatcgggt
    28441 agaaaccacg ctgttgcatg agttgatgca aaatctcttt cgcttcagct tcacgtccgc
    28501 gttccagcaa taaatccgcc tgccagtaac gccattcatc tttctctttt gcttccatcg
    28561 gcagacgcgc cagccaggta ttcaggccgc gacgatcgcc ggtgccaagc gccattcgca
    28621 cacggcgttc aatcaacgac gtggattgcg agcgcataat ggcgtcatcg cgccatttcg
    28681 cctgctcatc ggtgacatca ttgcccatca aacgccaggc gacgatatcg cgcagctcct
    28741 gaatctgatc ttcattaagc tgctgcgcct gggcaagcga cgggatcatc agccgtgcat
    28801 tttcggcatc ctgccgcgcc acactggcaa acgccaccgc cgccatttga cgggtaaaat
    28861 cggtcgcgcc ggttgtacgc gcgaaggtca gtaccgtatt agggttgttc gccagtgaaa
    28921 tgattgccga ggcgatagtc tggtaatcgg caggcatctg ccctgccagc actgttacca
    28981 ggcctgtgtt acccgctttc atcgccagac ggatacgctc taaatacgcc agcggatctt
    29041 gtttacctga cgcacgccag acgctaaata acttgtcaca ggcgttaggc tggctcttgc
    29101 cggttagcca cagctctttc gccccttgcc aggcttcttc actctgcccg gtgttccatt
    29161 tcgcatagta gtaattacat tgcgcttcgg tagttccggg cttttccggg ctaaaggcta
    29221 acaagccacg ccagtcttca cgccgcgcca gttcattgac gaaacgagat tgcagcgtgc
    29281 gagcgggagg aagcgtgggg ttagcgcgaa caaagttagt gaccgtcacc gccggttgat
    29341 tcatcaggtc atcggtgatc tggcggtatt ccaggtaggg ataaagcgga taatccttca
    29401 gtccaggcat catttgttcg accacatcca tctgtcgatt atcccaggcc tgcttgattt
    29461 gcgcgtaacg gctacgctgc tcatccagtg agtcggctcg cgccacgctg ctgaccgtca
    29521 gcagacagac accggcagcc aacagccgcc aggtaacttg tttggctttt tccacaagcg
    29581 catcctctaa gtgtaaatac atcaatgcag cgtcataccg cgtaatagat ttatgctaac
    29641 cagtcattgc cgtttacgcc acgttacgga cactttttta cttttactgc gaaggtgatc
    29701 gaccaactgg gattaggcgg ggaaacaggc tacacttcgc ctttgaaaac tcatcatcac
    29761 gataaacata gaggcgaagt ccaacgtggc tcaattcgtt tataccatgc atcgtgtcgg
    29821 caaagttgtt ccgccgaaac gtcatatttt gaaaaacatc tctctgagtt tcttccctgg
    29881 ggcaaaaatt ggtgtcctgg gtctgaacgg cgcgggtaag tccaccctgc tgcgcattat
    29941 ggcgggcatt gataaagaca tcgaaggtga agcgcgtccg cagccagaca tcaagattgg
    30001 ttatctgccg caggaaccgc agctgaaccc ggaacacacc gtgcgtgagt ccattgaaga
    30061 agcggtttca gaagtggtta acgccctgaa acgcctggat gaagtgtatg cgctgtacgc
    30121 cgatccggat gccgattttg acaagctggc cgctgaacaa ggccgtctgg aagagatcat
    30181 tcaggctcac gacggtcata acctgaacgt acagctggag cgtgcggcgg atgcgctgcg
    30241 tctgccagat tgggacgcga aaatcgctaa cctctccggt ggtgagcgtc gtcgcgtggc
    30301 gttgtgccgc ctgctgctgg aaaaaccaga catgctgctg ctcgacgaac cgaccaacca
    30361 cctggatgcc gaatccgtgg cgtggctgga acgcttcctg cacgacttcg aaggcaccgt
    30421 ggtggcgatt actcacgacc gttacttcct cgataacgtt gcaggctgga tcctcgaact
    30481 ggaccgcggt gaaggtattc cgtgggaagg taactactcc tcctggctgg atcagaaaga
    30541 tcagcgtctg gcgcaggaag cttcacaaga agcggcgcgt cgtaagtcga tcgagaaaga
    30601 gctggagtgg gtacgtcagg gaactaaagg cagccagtcg aaaggtaaag cacgtctggc
    30661 acgctttgaa gagctgaaca gcaccgaata ccagaaacgt aacgaaacca acgaactgtt
    30721 tattccacct ggaccgcgtc tgggcgataa agtgctggaa gtcagcaacc tgcgtaaatc
    30781 ctacggcgat cgcctgctga ttgatgacct gagcttctcg atcccgaaag gggcgatcgt
    30841 cgggatcatc ggtccgaacg gcgcgggtaa atcgaccctg ttccgtatga tctccggtca
    30901 ggaacagccg gacagcggca ccatcacttt gggtgaaacg gtgaaactgg catcggttga
    30961 tcagttccgt gactcaatgg ataacagcaa aaccgtttgg gaagaagttt ccggcgggct
    31021 ggatattatg aagatcggca acaccgagat gccaagccgc gcctacgttg gccgctttaa
    31081 ctttaaaggg gttgatcagg gtaaacgtgt tggcgaactt tccggtggtg agcgcggtcg
    31141 tctgcatctg gcgaagctgc tgcaggttgg cggcaacatg ctgctgctcg acgaaccaac
    31201 caacgacctg gatatcgaaa ccctgcgcgc gctggaaaac gccctgctgg agttcccggg
    31261 ctgtgcgatg gttatctcgc acgaccgttg gttcctcgac cgtatcgcca cgcacatcct
    31321 ggactaccag gatgaaggta aagttgagtt cttcgaaggt aactttactg agtacgaaga
    31381 gtacaaaaaa cgcacgctgg gcgcagacgc gctggagccg aagcgtatca agtacaagcg
    31441 tattgcgaag taatgcgtaa aatgccggat gcggcgcgaa cgccttatcc ggcctacaaa
    31501 accagcaatt tcaatacgtt gtaaaactgt aggcctgata agacgcggta agcgtcgcat
    31561 caggcattga gcaccgaatg ccggatgcgg cgtgaacacc ttttccggtc tacaacagcc
    31621 ttagcatcct caatttcgtg gcgggtcacc cgtgcaggtt ttttcaaacc ttgtttaatc
    31681 tcgacagcct cttccagaga ggttttcaac tcatcaaaaa aactcatttt atacctcgcc
    31741 tttgagcagt ttcgtcacgg ttatccttgc tcccccatca tctcccgcac cagctccacg
    31801 cagcgcagga aacggctgtc gtaatcgtcc tcttcaaccc gcacgaattc gatattgttc
    31861 tcttccagca tctccaccag caagttctgg aactctttgc gatccaccga actgccgagg
    31921 ctgcgtaaac catccgccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa
    31981 cggtattcat caatcagcgc ctgcacgaac ggatgctcac gcccttcgta ctttttgcag
    32041 aacgcctggg tagtgacaaa atcggtgtca ataaacgcca ctttattggc gtatttcact
    32101 gcaaaatcaa tgtattgtgc gtggcccaac gcgattttat cgtagtcgga atactgcaat
    32161 gcgatctcat caccgccgag gtgtgaaaag acataatcgc ggccatattc ccacgcactg
    32221 gtggtgttga agatattggc aagtttgttt accagggtgg atttaccgct cgactcgccg
    32281 ccaaggatcg ccacggtacg cacaaaaaac ggcttcactt cggtaggaat atattcccag
    32341 tagcggaacg ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga
    32401 tcaaccagca ccgtctcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc
    32461 gaggtgtaga tcaggtccgg ctggatcccc ttttcagcca taaacttttt gatgccgttg
    32521 ctccacacat cccagccatg cggatacggc tccatgccct cttcgttgaa agcatgaata
    32581 cggatatttt tctgatattt aaaggtttgc aatagccagc gcagacgatc cggcaccgtg
    32641 ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc
    32701 ataatgatat gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg
    32761 gtatgcagcg ggtagaactt accgaatacg acaccaatgg ttttcttctg ccggggaaat
    32821 tcaagcccca aaaaacggtg caatgcctcc agcttttgcg cgctggggct tttaattttg
    32881 gcattcagta actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt
    32941 agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc
    33001 tccgtatctc tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc
    33061 caaaaatctg catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc
    33121 ggcgaaaacc gtgtttcgcc gcttcagaaa tccgctcctg gccactgggc accgggcgga
    33181 tctcccctgc cagcccgact tcaccaaaca ccaccagatc ctgtggcagc ggtctgtcac
    33241 gcaggctgga aaccatcgcc agcagtaacg ctaagtcggc gctggtttcg gttaccttca
    33301 cgccgccgac cacgttcaca aacacatcct gatcggccat ttgcagacca ccgtgacggt
    33361 gcaagacagc cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg
    33421 gattcgccat tatcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc
    33481 cttcccacac caccatcacg gagctgccgg aagtcacttc atctccgcga cttaagaaaa
    33541 ttgccgaagg gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct
    33601 cattcaccgc gccgaagcgg tttttatggc tgcgtaaggt gcgaaaacgg gagtcggcat
    33661 cgccatccag aagcaccgaa cagtcgatac agtgttccag cactttcggg ccagccagcg
    33721 aaccatcttt ggttacgtgt ccaaccatga caatcgccac accgcgcgtt ttggcgaaac
    33781 gcgtcagata agccgccgtt tcacgcacct gcgccacgct accaggcgat gattgtacat
    33841 ccgccatatg catcacctgg atcgagtcaa ttaccatcag cttcggttgc tcttcttcgg
    33901 caatcaggca gatctgctcg atgctggttt ccgacaacat attgagattg tcagtcggca
    33961 ggccaaggcg atgagcgcgc attgccacct gttgcagcga ctcttcgccg gtgacataca
    34021 gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag caacagcgtg gatttccccg
    34081 caccagggtt accgccaatc agaatggcac ttcctggcac cacgccgccg cctagtacgc
    34141 ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga
    34201 gtttctggac ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg
    34261 ccaccgttgg cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac
    34321 tgcactgccc ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt
    34381 ttggagcttt tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tgacgggcag
    34441 gcaattactt ctgattcagg ctgccagaga ggatgcagaa tacccccatc aggtcagcgt
    34501 gacggatggt gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca
    34561 gccctgctgc tttgatcatc ggcaggtcat tagctccatc gccaatcgcc acggtctgcg
    34621 ccagcgggat ttcatactcc tgcgcgaggc gagtcagagt tttcgctttg tactgtgcgt
    34681 ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg
    34741 ctagcacggc tgtcaggcgc agcttgtcgc gcaggtattc agcaaagaaa gtaaagccgc
    34801 cggaggcaat cgccactttc cagcccagcg tttccagctt gagcaccagc tgcgttaagc
    34861 ctggcatcag cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca
    34921 gcgtcgccac gcggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt
    34981 cggttacctc cgctaccatc tcgcccgttc cggccagctt ggcaatttca tcaatacatt
    35041 caatctggat ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg
    35101 ggattttccc cagcggggcg acatccagct gcgcttcgtg ggccaggcgt gtagcccgtg
    35161 cggtgagtga acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa
    35221 tcaccatcgc cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac
    35281 gaccatacag cagccagccg ctacgagctg cgtggtaatc cagtggcatc acttcatcac
    35341 cacttaatga aagaggcaga cccggccata aagagacatc ttcaggcagg tcgcaccagg
    35401 taatgttagg cattaaggct cctgtaaaat cgttcgaagc agggaaaata acgcatgagg
    35461 ctaccttgta tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat
    35521 ggctcgcaca aaactgaaat tccggctaca tcgggcagtg attgtcctgt tctgtcttgc
    35581 cttgttagtg gcactgatgc agggagcgtc atggtttagt caaaaccacc agcgacagcg
    35641 taatccacag ctggaagaac tggcccgcac cctggcgcgt caggtgacgc tgaacgttgc
    35701 accgctgatg cgtaccgact caccggatga aaaacgcatt caggcgatcc tcgatcagtt
    35761 aacggatgaa agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc
    35821 acgttctggc gaaagcgtcg aagtgcgcga tcggctggcg ctcgacggta aaaaagcagg
    35881 cggctatttt aaccagcaga ttgtcgagcc aattgcgggt aaaaacggac cgctcggcta
    35941 tctgcgcctg acactcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac
    36001 cactaacatt ttacgcctga tgttgctgct ctcactggca atcggcgtag tgctgacccg
    36061 cacgcagcta cagggtaaac gcacccgctg gcagcaatcg cccttcctgt taaccgccag
    36121 caaaccggtg ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa
    36181 ggaaatcgtt atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa
    36241 cctggcggtg gaagagtgta tttttcgcca aatgcccgcc acgcagcgcg ttctgtttct
    36301 ctggcgcaat gccgacacgg tagtaattgg tcgcgcgcag aacccgtgga aagagtgtaa
    36361 tacccggcgg atggaagaag ataacgtccg cctggcgcgg cgcagtagcg gtggcggcgc
    36421 ggtgttccac gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga
    36481 taaaactatc tccacgtcga ttgtgctcaa tgcgctgaac gcgctcggcg tcagcgccga
    36541 agcgtccgga cgtaacgatc tggtggtgaa aaccgtcgaa ggcgaccgca aagtctcagg
    36601 ctcggcctat cgcgaaacca aagatcgcgg cttccaccac ggcaccttgc tactcaatgc
    36661 cgacctcagc cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg
    36721 cattacttca gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggctcaccca
    36781 tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg agcacgtgga
    36841 agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc
    36901 ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc atctgctgga
    36961 tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac
    37021 ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg
    37081 actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt
    37141 tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt
    37201 aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg gaaaaccagg
    37261 aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc gccgtgcgta
    37321 tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc
    37381 taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atggcagaaa
    37441 tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat
    37501 gaatagattc gtgaatagat acttgaatgt attttatttt ctatccatat gaaaaataat
    37561 agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg agcagtcccc
    37621 cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt tatcgcccag
    37681 cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc
    37741 agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca gggctttcgc
    37801 gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc cgaggatgcc
    37861 gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa
    37921 caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg cgttacgcac
    37981 catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc ggttaacttt
    38041 ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt gcggcagaac
    38101 tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga tcagttcttt
    38161 ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa ttttgcggcc
    38221 tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc
    38281 aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc
    38341 agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat cctttttgtg
    38401 acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc tttgccatat
    38461 tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc gaaggtttca
    38521 cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg aatgtgttca
    38581 cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt gaggatcagg
    38641 atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa cagttccaga
    38701 cccgcagcat aaccggcgac gtcgcgacgg tggccccagg aagagtcgaa gtcaacgaag
    38761 ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt ggcgtcaaac
    38821 agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc gtagatgtct
    38881 gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt ctgcagcacg
    38941 gtcggtgctg gcggctcaac agccaggtcg tgacggttac cggtacgctg gaagttacca
    39001 gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc gccgttggtc
    39061 agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa agtttcttca
    39121 tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt gccggttttc
    39181 atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca gttaccgagg
    39241 taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg gaagctgttt
    39301 tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat ttcccagtga
    39361 ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc gccgataact
    39421 tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg tgctttcgcc
    39481 agccccagac ggatcagatt tggcagattg agcggccctt tacgaccatt atcagcttcg
    39541 cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc accaaagcgt
    39601 tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa tgcacgtttc
    39661 atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata ccgttattcg
    39721 ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag tttaattgcc
    39781 gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag gataaccgcc
    39841 agcggacgct gaccgtctac atggtcgccc agacgcgcca tatcagtaaa gccgacgctg
    39901 taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaaccac tgccatcccc
    39961 agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac tgctttcgtc
    40021 agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg gcctttttgt
    40081 gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac cgcctgcaat
    40141 ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcagcat ctccacgcac
    40201 agcaccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag gaactgcacc
    40261 gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt catgtcggtg
    40321 agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc ttcggcaagg
    40381 gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac gtccatcacc
    40441 agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat cagcgggatg
    40501 gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc cggagccagt
    40561 gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg gaaacggttg
    40621 tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt accgccagta
    40681 tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc gaccatcggc
    40741 cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc aacaatcggg
    40801 ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat cgccatggtc
    40861 agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc gagggcggca
    40921 atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg aatttcttca
    40981 tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag aaacaaggta
    41041 acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc cttatccggc
    41101 ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt aaagcatctt
    41161 acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag caggcttgcc
    41221 agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc accgaacagt
    41281 tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc gcccgccggt
    41341 ttgaaaccaa cggttttttc tacgcccata taacggatca cttccatcat gatgcgagcg
    41401 ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa gtccgcaccc
    41461 gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag ttcgccggtt
    41521 tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca ggctttcacc
    41581 aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa caccacgtca
    41641 acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat ttcgatgtcg
    41701 tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt gccctgctct
    41761 ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc ggcggtattg
    41821 ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt gtcgtcgtca
    41881 ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt cagatcagtc
    41941 atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt agtattctaa
    42001 catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac ttacaagttt
    42061 gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc gtcagtggtg
    42121 gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg catcaccttc
    42181 ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac aacgttgcca
    42241 gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc tggcactgga
    42301 agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg cgctggatga
    42361 aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct atgtcttacc
    42421 cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag gcgcgaaaga
    42481 tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt cggtcacatc
    42541 gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta gaattctaac
    42601 acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca gaaccctgcc
    42661 agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc gcgcgatctt
    42721 acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg aaatcacccc
    42781 tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac cgcacatcgc
    42841 caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg tgagctgtta
    42901 caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc tgaaagccat
    42961 tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc actactgcca
    43021 gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat cacacttctt
    43081 tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg tgccaatgtt
    43141 tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag ggattgccgc
    43201 gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg cgcaactggc
    43261 ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca tgttacacgc
    43321 acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc cgtatggcga
    43381 tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt attattattc
    43441 cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc aggcactggt
    43501 acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca atgtcagcgg
    43561 taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag aaaaatatcg
    43621 cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac gcaacactcg
    43681 tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct ttagtctgta
    43741 agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg tttttgcagg
    43801 gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt tgcaatgact
    43861 gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac ggcaaagtgg
    43921 tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt ccgcaacatg
    43981 ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg cgcaaagcgg
    44041 tgctgtttat tgaagggata acggtaagcg gcggcgaagt cacgacccaa ctgccgtttg
    44101 tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc acctgtctgg
    44161 tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg gtgtgtgacg
    44221 gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta accggacgcg
    44281 ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag ctggcggaac
    44341 tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa gaactggcgg
    44401 cgtttatcaa gggacttgga gatgttccgg ttcgcctgaa cgcgtttcat gcccacggcg
    44461 tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag ccgttggctg
    44521 atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat ttgtgacatg
    44581 ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg tcaagcgtcg
    44641 catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg gcctacggtt
    44701 cggcacaaac ctgtaggcct gataagacgc gttaagcgtc gcatcaggca ttatgcgccg
    44761 actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac cgcatacgtg
    44821 ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc gcaaagcacg
    44881 gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc gttgagcggc
    44941 atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc gatgacatcg
    45001 cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt gtagcccagc
    45061 tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac accagcacgc
    45121 ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg tgaatgcagg
    45181 atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa ccactgctgc
    45241 ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac cgccaccacc
    45301 ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc gctatgtttt
    45361 tccaacatac caggatgcaa gcccaatgcg gcatacagcg gttgataatt ttccgctaac
    45421 gcctgcacgc gggcaaaatt ttccgcctct gtcgccggaa caatgatctt gcctacgccc
    45481 gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg gaaatcaaaa
    45541 tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta aatgtcgggt
    45601 cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg actggcgcag
    45661 gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta agcggcgctt
    45721 tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa cgcccaagtt
    45781 tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt aatggcttcg
    45841 gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat tgctgaatgt
    45901 cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg ctactgtcac
    45961 ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt gacggcacag
    46021 tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc ggaatcgcat
    46081 cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta cgataaaagc
    46141 caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt ggtaaaaagt
    46201 aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg ccgctgtcaa
    46261 acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc tccaccagcc
    46321 agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa aattcgcgtt
    46381 ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag ataaacgccg
    46441 agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta aactgcgcgc
    46501 gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg ccttcgcaca
    46561 ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata ttaccaagcg
    46621 ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag aaagacaaag
    46681 taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg gctttggata
    46741 aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa actcactagg
    46801 aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt accggccaga
    46861 ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat gccccaacga
    46921 aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg tgaaaatcac
    46981 tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc gggaggacga
    47041 attacgacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47101 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47161 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47221 agtttagctt tgatttcact ggtggtggcg gtgtcacccg cgtagtcctt caccgagcct
    47281 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47341 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47401 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47461 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47521 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47581 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47641 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47701 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47761 tcacaaaagc gatgggtgat gaataacgac cattacagtc tctgaatcag ttattaatat
    47821 cggtagaatg acaatcgacg gctttagata ctgatatcta cgcattgaac ggtatttaac
    47881 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47941 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    48001 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48061 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48121 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48181 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48241 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48301 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48361 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48421 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48481 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48541 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48601 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48661 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcgggtcca
    48721 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48781 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48841 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48901 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48961 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    49021 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49081 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccag gtgatcggtg
    49141 cgcagccaat tttcagctcg ttctcaactt catcgagaag ttccatcggg tcgcggatat
    49201 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49261 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49321 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49381 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49441 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49501 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49561 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctcttgca
    49621 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49681 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49741 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49801 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49861 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49921 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49981 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    50041 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gccacgaatg gcgttaagca
    50101 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50161 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50221 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50281 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50341 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50401 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50461 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50521 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50581 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50641 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccaaca ccacttgcgt
    50701 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50761 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50821 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50881 gttgccataa cgcggcgcgt gccgttgggt ttacccgtaa ttcggtgagc gccggtgatg
    50941 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    51001 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51061 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51121 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51181 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51241 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51301 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51361 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51421 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51481 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51541 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51601 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51661 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51721 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51781 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51841 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51901 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51961 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    52021 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52081 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52141 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52201 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52261 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52321 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52381 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52441 acctacaaaa cctgcacgct agtttcctga tggacatttt tccagcaatt acacatcagg
    52501 cgataattaa ctattgacga aaagctgaaa accactagaa tgcgcctccg tggtagcaat
    52561 tctttttaag aattgatggt atgcgaaggt ggcggaattg gtagacgcgc tagcttcagg
    52621 tgttagtgtc cttacggacg tgggggttca agtccccccc ctcgcaccaa ttatctttac
    52681 ttcctttctt gtttcttccc tgttttgtca tccctgattc aaattcatta tcatcagcat
    52741 gatgccagca ataagcgcca ggcacgatgg tagcaaaatg taatgccgca actgtttgtg
    52801 atacagcatc accggcgtca atactaatcc catcagaatg acaatccgcc actgatccgg
    52861 cgataaatcc gcatacatca gccctgcaat cagcaatccc ggcagcaaca ctccccagcc
    52921 actgcccagc gtacgttgca acatggtttc atctctttca ttgataatga aaaccaatat
    52981 catatgataa tttttatcat ttgcaagcca gagaaatccc ttgctatcga gtaaacctat
    53041 cgataggatt agcaatcatt atcatttaga ttactatcac gattatggcc tatcgttccg
    53101 caccgctcta tgaagatgtc atctggcgaa cgcatctcca gccgcaggat gctgggcttg
    53161 cacaagccgt acgggcgatg atcgccaaac atcgtgaaca tttgctggag tttatccgcc
    53221 tggatgaacc cgctccgctt aacgccatga cgctggcgca atggtcatca ccgaatgcgc
    53281 taagttctct actggcggtc tattccgatc atatctatcg caaccaacca acgatgatcc
    53341 gcgagaacaa accgctgatc tcactatggg cacaatggta tatcggcctg atggtgccac
    53401 cattaatgct ggcgctactg acgcaggaaa aagcattaga tgtgtcgccg gaacatttcc
    53461 atgctgagtt tcacgaaacc ggacgcgtcg cctgtttctg ggtcgatgtg tgtgaagata
    53521 aaaacgcaac accacattcg ccgcagcagc gaatggaaac gttaatcagc caggcgctgg
    53581 ttccggttgt gcaagcacta gaagcgaccg gagagatcaa cggcaaactt atctggagta
    53641 ataccggtta tttgatcaac tggtatctca ctgagatgaa gcaactgctc ggcgaggcta
    53701 ctgttgaatc gctgcgccat gccctctttt ttgagaaaac gctcacgaac ggtgaagata
    53761 atccactctg gcgtaccgtg gtactgcgcg acggcctgct ggtgcgccgc acttgctgcc
    53821 agcgttatcg cttaccggat gtgcagcaat gtggctattg tacgctgaaa tgaaagcact
    53881 gccggggaag taaacccggc atcatgcgga ttaataggga tgcaacacat tacttgcttc
    53941 gcaatgccgc atacataaca gaatatctgc ggcctccaac aaccctgcat cagaactgac
    54001 gcccagtttc gacatcacat taaatttatg cgctcggatc gttttgatat tacgtttaag
    54061 ctgctcggca atttgtgtca ttgagtagcc acgcgacata aagcgcaata tttctctctc
    54121 cgtcgggctt aacgtccggc tttggttaat gtaccactga ttgttcagtc ggtcggtcgc
    54181 ctgacgtaca ccatttaatg acaaaaagag ttcctgatga aaaatctcca gcgttgacgc
    54241 tttacttaat acaccgtcca gcggtgatgg cgacaatgaa ccaatcagcc gagcttcaat
    54301 atcatcatcc acaataacta aacgccgggt acgcggaaac ttaatcgcca gctcagtcag
    54361 gcaagataat ccctctctgc gctcacttct catggcagaa aaagaaaaaa tgaccgccgc
    54421 aaacgagatc cgcgacatgg catcctgaaa tgactcctga ctggtatagg tatggagctt
    54481 ataagggttg cccgcaaact ttctgaataa actctcaata ccgatactac tcatgacgca
    54541 tttttctatg agtgcgacat ttctcttctt aattcggctg tgttccattc tgcggcatct
    54601 ccataaacag aaggcaagtt aagttctctt gcaccctgcg cgctatttat ccatgcatac
    54661 atttcagcgt tgctgtgaat tgccagtcga cgcatcgcac tgtttttttg ggcgctgata
    54721 gttttattac ttttcttaag caatgaagct atctggttga tgccccatcc tttacctaat
    54781 agccgcagta ccttgcgctc tgaaagagtg agaatgacgg agcgatagct aaaatcatga
    54841 gtttcagtca ttgccggaga ggtcagcatg gcgctgatac gctctgcatg cgtattgccg
    54901 gaacgtacgg tcttgaccag attctcaatg ggttcaacat cagacaataa cgtcgccgta
    54961 gggcacatga gcagttctac agcctgggaa taccaggagc gtgacaccat gaaaacccag
    55021 tgaatttccc gatattgtga gattaaagaa taataatgtt cacaaacaga acgtggatct
    55081 tcactttcac cggctaaatc agcaatgact
//
LOCUS       DADWTH010000019        53211 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.019, whole genome shotgun sequence.
ACCESSION   DADWTH010000019 DADWTH010000000
VERSION     DADWTH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.019"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(11..322)
                     /locus_tag="JNY02_001127"
     CDS             complement(11..322)
                     /locus_tag="JNY02_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBB9432743.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JNY02_001128"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JNY02_001128"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JNY02_001129"
     CDS             701..1054
                     /locus_tag="JNY02_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBB9432744.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNY02_001130"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNY02_001130"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBB9432745.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FINVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JNY02_001131"
     CDS             complement(2810..3280)
                     /locus_tag="JNY02_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBB9432746.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNY02_001132"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNY02_001132"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBB9432747.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNY02_001133"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNY02_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBB9432748.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNY02_001134"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNY02_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBB9432749.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JNY02_001135"
     CDS             complement(5283..8432)
                     /locus_tag="JNY02_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBB9432750.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNY02_001136"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNY02_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBB9432751.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JNY02_001137"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JNY02_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBB9432752.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JNY02_001138"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JNY02_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBB9432753.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISGLLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNY02_001139"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNY02_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBB9432754.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNY02_001140"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNY02_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBB9432755.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JNY02_001141"
     CDS             14911..15288
                     /locus_tag="JNY02_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBB9432756.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JNY02_001142"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JNY02_001142"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBB9432757.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JNY02_001143"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JNY02_001143"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBB9432758.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWTAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="JNY02_001143"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="JNY02_001144"
     CDS             18099..18428
                     /locus_tag="JNY02_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBB9432759.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JNY02_001145"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JNY02_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBB9432760.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JNY02_001146"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JNY02_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBB9432761.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JNY02_001147"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JNY02_001147"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBB9432762.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JNY02_001148"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JNY02_001148"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBB9432763.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNY02_001149"
                     /pseudo
     CDS             complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNY02_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="JNY02_001150"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="JNY02_001150"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBB9432764.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="JNY02_001151"
     CDS             complement(25482..27158)
                     /locus_tag="JNY02_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBB9432765.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="JNY02_001152"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="JNY02_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBB9432766.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALPGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="JNY02_001153"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="JNY02_001153"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBB9432767.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNY02_001154"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNY02_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBB9432768.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="JNY02_001155"
     CDS             complement(31206..32000)
                     /locus_tag="JNY02_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBB9432769.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALLVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="JNY02_001156"
     CDS             32138..32479
                     /locus_tag="JNY02_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBB9432770.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNY02_001157"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNY02_001157"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBB9432771.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAAFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="JNY02_001158"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="JNY02_001158"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBB9432772.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVLGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="JNY02_001159"
     CDS             36395..37687
                     /locus_tag="JNY02_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB9432773.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /gene="cueR"
                     /locus_tag="JNY02_001160"
     CDS             37812..38219
                     /gene="cueR"
                     /locus_tag="JNY02_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBB9432774.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="JNY02_001161"
     CDS             complement(38220..38678)
                     /locus_tag="JNY02_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBB9432775.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="JNY02_001162"
     CDS             complement(38675..39592)
                     /locus_tag="JNY02_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBB9432776.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="JNY02_001163"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="JNY02_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBB9432777.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EVRYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="JNY02_001164"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="JNY02_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBB9432778.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JNY02_001165"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JNY02_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBB9432779.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JNY02_001166"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JNY02_001166"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBB9432780.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="JNY02_001167"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="JNY02_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="JNY02_001168"
     CDS             43551..44237
                     /locus_tag="JNY02_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB9432781.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46648
                     /locus_tag="JNY02_001169"
     CDS             44234..46648
                     /locus_tag="JNY02_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9432782.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JNY02_001170"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JNY02_001170"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBB9432783.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JNY02_001171"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JNY02_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBB9432784.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="JNY02_001172"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="JNY02_001172"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBB9432785.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="JNY02_001173"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="JNY02_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBB9432786.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="JNY02_001174"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="JNY02_001174"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBB9432787.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53211
                     /locus_tag="JNY02_001175"
     CDS             52938..>53211
                     /locus_tag="JNY02_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBB9432788.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12818 a        13707 c        14022 g        12664 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttta tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatt
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc ggactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tggacggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtattgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgccttcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaaaattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaatcata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca aggggcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    28261 gccagcagaa ctgcgccaaa actgcccggc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 ttaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgagca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gactacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atgcggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagaaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca caggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacta ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 catcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatac
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagtacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt cgcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 gggtcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atatccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acggacgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tgtaactatg attttcttca tgtgctagat gaggactgcc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aacaaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggcggc gcgttcatcg ttattcatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcg
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gtaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gcgttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt taaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgatgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ttggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt c
//
LOCUS       DADWTH010000020        49573 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.020, whole genome shotgun sequence.
ACCESSION   DADWTH010000020 DADWTH010000000
VERSION     DADWTH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.020"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1224
                     /locus_tag="JNY02_001176"
     CDS             <1..1224
                     /locus_tag="JNY02_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBB9432789.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="JNY02_001177"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="JNY02_001177"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBB9432790.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="JNY02_001178"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="JNY02_001178"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBB9432791.1"
                     /translation="MSTLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="JNY02_001179"
     CDS             2714..3427
                     /locus_tag="JNY02_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBB9432792.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="JNY02_001180"
     CDS             complement(3399..4076)
                     /locus_tag="JNY02_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBB9432793.1"
                     /translation="MLTLNKISYRWPGAATDCLCGISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="JNY02_001181"
     CDS             complement(4070..4747)
                     /locus_tag="JNY02_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB9432794.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="JNY02_001182"
     CDS             complement(4735..5118)
                     /locus_tag="JNY02_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBB9432795.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="JNY02_001183"
     CDS             complement(5134..5442)
                     /locus_tag="JNY02_001183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432796.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="JNY02_001184"
     CDS             complement(5443..6021)
                     /locus_tag="JNY02_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB9432797.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="JNY02_001185"
     CDS             complement(6044..6475)
                     /locus_tag="JNY02_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB9432798.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="JNY02_001186"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="JNY02_001186"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBB9432799.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="JNY02_001187"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="JNY02_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBB9432800.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="JNY02_001188"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="JNY02_001188"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBB9432801.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="JNY02_001189"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="JNY02_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBB9432802.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="JNY02_001190"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="JNY02_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBB9432803.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="JNY02_001191"
     CDS             12291..13031
                     /locus_tag="JNY02_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBB9432804.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="JNY02_001192"
                     /pseudo
     CDS             13198..14148
                     /locus_tag="JNY02_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNY02_001193"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNY02_001193"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBB9432805.1"
                     /translation="METQWTRMTADEAAEIILHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNY02_001194"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNY02_001194"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBB9432806.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNY02_001195"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNY02_001195"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBB9432807.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNY02_001196"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNY02_001196"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBB9432808.1"
                     /translation="MPNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="JNY02_001197"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="JNY02_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBB9432809.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="JNY02_001198"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="JNY02_001198"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBB9432810.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="JNY02_001199"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="JNY02_001199"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBB9432811.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="JNY02_001200"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="JNY02_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBB9432812.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="JNY02_001201"
     CDS             23768..23827
                     /locus_tag="JNY02_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBB9432813.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23924..24526)
                     /locus_tag="JNY02_001202"
     CDS             complement(23924..24526)
                     /locus_tag="JNY02_001202"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBB9432814.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24547..24730)
                     /gene="ssrS"
                     /locus_tag="JNY02_001203"
     ncRNA           complement(24547..24730)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JNY02_001203"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="JNY02_001204"
     CDS             complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="JNY02_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBB9432815.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25269..25847
                     /gene="ygfB"
                     /locus_tag="JNY02_001205"
     CDS             25269..25847
                     /gene="ygfB"
                     /locus_tag="JNY02_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBB9432816.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25873..27198
                     /gene="pepP"
                     /locus_tag="JNY02_001206"
     CDS             25873..27198
                     /gene="pepP"
                     /locus_tag="JNY02_001206"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBB9432817.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHARGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27195..28373
                     /gene="ubiH"
                     /locus_tag="JNY02_001207"
                     /gene_synonym="visB"
     CDS             27195..28373
                     /gene="ubiH"
                     /locus_tag="JNY02_001207"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBB9432818.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28396..29598
                     /gene="ubiI"
                     /locus_tag="JNY02_001208"
                     /gene_synonym="visC"
     CDS             28396..29598
                     /gene="ubiI"
                     /locus_tag="JNY02_001208"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBB9432819.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVASIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(29672..29755)
                     /locus_tag="JNY02_001209"
     CDS             complement(29672..29755)
                     /locus_tag="JNY02_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432820.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30046..31140
                     /gene="gcvT"
                     /locus_tag="JNY02_001210"
     CDS             30046..31140
                     /gene="gcvT"
                     /locus_tag="JNY02_001210"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBB9432821.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31164..31553
                     /gene="gcvH"
                     /locus_tag="JNY02_001211"
     CDS             31164..31553
                     /gene="gcvH"
                     /locus_tag="JNY02_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBB9432822.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31672..34545
                     /gene="gcvP"
                     /locus_tag="JNY02_001212"
     CDS             31672..34545
                     /gene="gcvP"
                     /locus_tag="JNY02_001212"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBB9432823.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34812..35555
                     /locus_tag="JNY02_001213"
     CDS             34812..35555
                     /locus_tag="JNY02_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB9432824.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVVQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35612..37045)
                     /gene="bglA"
                     /locus_tag="JNY02_001214"
     CDS             complement(35612..37045)
                     /gene="bglA"
                     /locus_tag="JNY02_001214"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBB9432825.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37090..37401
                     /locus_tag="JNY02_001215"
     CDS             37090..37401
                     /locus_tag="JNY02_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBB9432826.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            37565..38224
                     /locus_tag="JNY02_001216"
     CDS             37565..38224
                     /locus_tag="JNY02_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBB9432827.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="JNY02_001217"
     CDS             complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="JNY02_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBB9432828.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39643..39909
                     /gene="sdhE"
                     /locus_tag="JNY02_001218"
     CDS             39643..39909
                     /gene="sdhE"
                     /locus_tag="JNY02_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBB9432829.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39890..40297
                     /locus_tag="JNY02_001219"
     CDS             39890..40297
                     /locus_tag="JNY02_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBB9432830.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="JNY02_001220"
     CDS             complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="JNY02_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBB9432831.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40970..41866
                     /gene="xerD"
                     /locus_tag="JNY02_001221"
     CDS             40970..41866
                     /gene="xerD"
                     /locus_tag="JNY02_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBB9432832.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41891..42601
                     /gene="dsbC"
                     /locus_tag="JNY02_001222"
     CDS             41891..42601
                     /gene="dsbC"
                     /locus_tag="JNY02_001222"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBB9432833.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42607..44340
                     /gene="recJ"
                     /locus_tag="JNY02_001223"
     CDS             42607..44340
                     /gene="recJ"
                     /locus_tag="JNY02_001223"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBB9432834.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44431..45529
                     /gene="prfB"
                     /locus_tag="JNY02_001224"
     CDS             join(44431..44505,44507..45529)
                     /gene="prfB"
                     /locus_tag="JNY02_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBB9432835.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45539..47056
                     /gene="lysS"
                     /locus_tag="JNY02_001225"
     CDS             45539..47056
                     /gene="lysS"
                     /locus_tag="JNY02_001225"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBB9432836.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47099..47647)
                     /gene="idi"
                     /locus_tag="JNY02_001226"
     CDS             complement(47099..47647)
                     /gene="idi"
                     /locus_tag="JNY02_001226"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBB9432837.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARILLSAFTQLK"
     gene            47702..47773
                     /locus_tag="JNY02_001227"
     CDS             47702..47773
                     /locus_tag="JNY02_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432838.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47770..47895)
                     /locus_tag="JNY02_001228"
     CDS             complement(47770..47895)
                     /locus_tag="JNY02_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432839.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47897..49345)
                     /gene="uacT"
                     /locus_tag="JNY02_001229"
     CDS             complement(47897..49345)
                     /gene="uacT"
                     /locus_tag="JNY02_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBB9432840.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11967 a        12403 c        13349 g        11854 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg tcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgccgcaa
     4021 agacaatccg ttgccgcgcc tggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatttctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 tgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcatt gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgtgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgcaggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ctaggttata
    15961 cgggacaccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttaggcat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gttaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta
    23881 aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt
    23941 tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca
    24001 ccaactgaca atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta
    24061 aggtccgatc ataaaaacca ccgcccattc ccaggcgctg accgtactca tcaaaggcga
    24121 ccagcggtgt gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt
    24181 ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga
    24241 acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc
    24301 gccagagttg ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag
    24361 cgaccgtatg cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac
    24421 ccatttccag ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga
    24481 tttcttgtcg ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat
    24541 aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt
    24601 gaatgtgtcg tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg
    24661 agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc
    24721 tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa
    24781 gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg
    24841 ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc
    24901 ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt
    24961 gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt
    25021 caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat
    25081 atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt
    25141 agacccatgg tctgcgaagg ggcgacgcta tagctaccct gatgagaaga gacaagccct
    25201 tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac
    25261 gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga
    25321 accaacaagg gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat
    25381 gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca
    25441 tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg
    25501 ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg
    25561 ttttcgatcg ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg
    25621 ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc
    25681 gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc
    25741 ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc
    25801 atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt
    25861 aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg
    25921 gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt
    25981 agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt
    26041 aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt
    26101 ctgtttaacc gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag
    26161 gatgccgcgc cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag
    26221 caactttatc aactacttaa cggcctggat gtggtttacc atgcccgggg cgaatatgca
    26281 tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat
    26341 ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc
    26401 aaatcgccag aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat
    26461 acacgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt
    26521 caccacgaat ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc
    26581 ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac
    26641 ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc
    26701 ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag
    26761 tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt
    26821 gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt
    26881 gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg
    26941 ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa
    27001 ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca
    27061 gaacaatatc gcggtatcgg cattcgtatt gaagacgaca ttgtgattac cgaaaccggc
    27121 aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct
    27181 gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg
    27241 cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg
    27301 cgccagagtc acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta
    27361 cctgtcagca actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca
    27421 tcactaccgt gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag
    27481 attaccaact ggcggcgctg gggcaggttg tcgaattgca taatgttggg caacggctgt
    27541 ttgcattgct gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg
    27601 ttgcccgtac tcagagtcac gttgaagtga cgctggagag cggcgagatg ctgacgggcc
    27661 gcgtgctgat agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt
    27721 ggcagcagga gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc
    27781 acgaaggccg cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt
    27841 ctgacggacg ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt
    27901 cgtggagtga cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc
    27961 aaattaccca cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac
    28021 cgattaccca tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg
    28081 ggcaagggtt taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg
    28141 cgcatgagcg cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc
    28201 gacagagcga tcgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca
    28261 accgttgggc accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca
    28321 ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt
    28381 tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg
    28441 ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc
    28501 gtaccggaac ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc
    28561 gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc
    28621 agttgctatc acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac
    28681 gatcaaagca tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac
    28741 gcgctgtgga acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta
    28801 cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta
    28861 acggcgcgtc tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat
    28921 attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgagcat tcgcacggaa
    28981 gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta
    29041 ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag
    29101 cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat
    29161 cgccttggct tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat
    29221 gcgcgccagt ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac
    29281 ccactggcgg ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa
    29341 ctgaaacggt tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat
    29401 gaacgtagcc gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat
    29461 ctcttttccg gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc
    29521 gacacgcttc ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg
    29581 tctgaatggc tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc
    29641 atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg
    29701 ttttcgcatt atattttcta atgccatgat tttttgattt agtgtttttt gacatttttt
    29761 tagctcttaa tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa
    29821 ttggtgtatt ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta
    29881 atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa
    29941 agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt
    30001 tcaccatgaa aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
    30061 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
    30121 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
    30181 atgtttgatg tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
    30241 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac
    30301 tcggggatgt tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact
    30361 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
    30421 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
    30481 gccgtgcaag ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt
    30541 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc
    30601 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
    30661 gatttctggc gtgcgctggt ggaggcgggc gttaagccct gcggtctggg ggcgcgtgac
    30721 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
    30781 cctttagccg ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc
    30841 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
    30901 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
    30961 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
    31021 gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
    31081 gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
    31141 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
    31201 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
    31261 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
    31321 gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc
    31381 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
    31441 acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agctagcgat gaaagcgaac
    31501 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt
    31561 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
    31621 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
    31681 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
    31741 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
    31801 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
    31861 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
    31921 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
    31981 gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc
    32041 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
    32101 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
    32161 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg
    32221 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
    32281 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
    32341 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
    32401 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
    32461 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
    32521 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
    32581 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
    32641 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
    32701 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
    32761 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
    32821 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
    32881 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
    32941 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat
    33001 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
    33061 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac
    33121 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
    33181 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
    33241 ccaatcactt ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
    33301 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
    33361 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
    33421 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
    33481 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
    33541 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
    33601 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
    33661 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
    33721 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
    33781 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
    33841 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    33901 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
    33961 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
    34021 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    34081 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
    34141 gaagaaatcg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    34201 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
    34261 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
    34321 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
    34381 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    34441 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    34501 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt
    34561 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
    34621 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
    34681 gaatatttac cctcaccccg accatctccc tgaaagggcg agggggaaaa gcgtgccaac
    34741 attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct
    34801 cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggtatc gggcgggcaa
    34861 ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc
    34921 acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc
    34981 aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg
    35041 atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg
    35101 aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc
    35161 tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta
    35221 tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg
    35281 cggcatcgaa aggggcgatt gatacgttaa ccaccggacg atcgctggaa gtcgccgcgc
    35341 aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg
    35401 gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac
    35461 aggcagaaga ggtcgtgcag gccattgtct ggctactaag tgataaagcc tcttacgtca
    35521 cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg
    35581 caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca
    35641 cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca gtaccgtcgt
    35701 catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta gtgaatgaca
    35761 cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg taagtcactg
    35821 cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc
    35881 tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc
    35941 tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc
    36001 agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa ccagagatcg
    36061 catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat
    36121 aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg atattaaatc
    36181 cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc tgcacatcgg
    36241 taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacatg
    36301 agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc
    36361 gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc acctggtaca
    36421 tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag taaccaaaca
    36481 gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag tatttgactt
    36541 tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca acgaatttac
    36601 ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg
    36661 ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg taaaacttca
    36721 gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc caggcgatgg
    36781 aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac
    36841 cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact tctttggtga
    36901 tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc ggtcctttgc
    36961 cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc cataagaaat
    37021 ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat tcttacagga
    37081 aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga tgacattctg
    37141 gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat
    37201 gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga agtcaccgca
    37261 acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga aaatatgacc
    37321 ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca attttatatg
    37381 attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat
    37441 ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac
    37501 tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt ctctggagta
    37561 agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa
    37621 cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt tgctactggt
    37681 tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg
    37741 cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc
    37801 aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta
    37861 cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat
    37921 ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc gattcaaaat
    37981 tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat
    38041 ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc
    38101 gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg
    38161 cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc
    38221 gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca ggcatcggag
    38281 cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg
    38341 ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg
    38401 cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg
    38461 tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
    38521 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac
    38581 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcac
    38641 tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
    38701 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    38761 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa ccagcttcaa
    38821 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    38881 tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    38941 accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc
    39001 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    39061 cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct
    39121 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
    39181 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
    39241 ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
    39301 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    39361 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    39421 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    39481 gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa ttgttgtgaa
    39541 ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    39601 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    39661 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    39721 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    39781 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
    39841 gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    39901 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    39961 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    40021 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta atgctcgcca
    40081 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    40141 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    40201 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    40261 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    40321 taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
    40381 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
    40441 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    40501 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    40561 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaataattt
    40621 taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcttggattt
    40681 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    40741 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    40801 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    40861 cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca
    40921 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    40981 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    41041 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    41101 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    41161 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    41221 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    41281 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    41341 gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    41401 taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    41461 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggggaaga
    41521 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    41581 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    41641 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    41701 tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    41761 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    41821 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    41881 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    41941 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    42001 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    42061 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    42121 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    42181 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    42241 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    42301 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    42361 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    42421 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    42481 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    42541 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    42601 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    42661 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    42721 caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag cggtgtcgaa
    42781 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    42841 gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
    42901 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    42961 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    43021 ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc ggttattgtt
    43081 accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    43141 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    43201 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    43261 attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtagc ggacgtcgtg
    43321 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    43381 aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc acaaaaacta
    43441 gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg gcgactggat
    43501 gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggagaagc gcgcgtgctg
    43561 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaggtt
    43621 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    43681 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgtatcaaa
    43741 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    43801 tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt agacacactc
    43861 taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    43921 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggcc
    43981 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    44041 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    44101 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    44161 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    44221 accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa gctcgatatc
    44281 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    44341 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    44401 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    44461 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    44521 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    44581 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    44641 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    44701 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    44761 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgttacct
    44821 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
    44881 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    44941 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg
    45001 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    45061 cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg atgatgatat
    45121 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    45181 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat
    45241 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg ccatgaagca
    45301 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    45361 ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    45421 ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
    45481 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    45541 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    45601 gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga acgatttccg
    45661 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    45721 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    45781 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    45841 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    45901 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    45961 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    46021 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    46081 taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    46141 tggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    46201 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    46261 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    46321 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    46381 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    46441 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    46501 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    46561 catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    46621 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    46681 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc
    46741 gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg
    46801 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct
    46861 ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta
    46921 cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg
    46981 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    47041 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    47101 atttaagctg ggtaaatgca gataatagta ttctggcttc gcgatttgtc gcctgcatca
    47161 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    47221 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    47281 cggcaaatac cggacacact tcattttcca caatgccact cggatcggtg gcgcggtagc
    47341 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    47401 ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa accgagttag
    47461 tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgcccct
    47521 tggcattaaa cagccaactg gaaaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    47581 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    47641 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    47701 aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt
    47761 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    47821 acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga atatagcgcg
    47881 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    47941 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    48001 catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg gttgcgagca
    48061 taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    48121 agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt
    48181 tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    48241 tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt
    48301 ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat
    48361 gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt
    48421 taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt
    48481 gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    48541 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    48601 taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc ccggataaat
    48661 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    48721 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    48781 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccagcggccc
    48841 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    48901 gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg gtgataaaac
    48961 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    49021 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    49081 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    49141 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    49201 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    49261 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    49321 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag aataaaaagc
    49381 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    49441 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    49501 aatgacaaga gaacctgata atggaattat ctgtaatttt ctcatattga tatttcatta
    49561 atcattctgg cat
//
LOCUS       DADWTH010000021        47765 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.021, whole genome shotgun sequence.
ACCESSION   DADWTH010000021 DADWTH010000000
VERSION     DADWTH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.021"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="JNY02_001230"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="JNY02_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBB9432841.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="JNY02_001231"
     CDS             complement(1899..2636)
                     /locus_tag="JNY02_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432842.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /locus_tag="JNY02_001232"
     CDS             complement(2839..3570)
                     /locus_tag="JNY02_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBB9432843.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3584..4093)
                     /gene="traS"
                     /locus_tag="JNY02_001233"
     CDS             complement(3584..4093)
                     /gene="traS"
                     /locus_tag="JNY02_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HBB9432844.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4090..6915)
                     /gene="traG"
                     /locus_tag="JNY02_001234"
     CDS             complement(4090..6915)
                     /gene="traG"
                     /locus_tag="JNY02_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBB9432845.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6912..8285)
                     /locus_tag="JNY02_001235"
     CDS             complement(6912..8285)
                     /locus_tag="JNY02_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBB9432846.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8272..8697)
                     /gene="trbF"
                     /locus_tag="JNY02_001236"
     CDS             complement(8272..8697)
                     /gene="trbF"
                     /locus_tag="JNY02_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBB9432847.1"
                     /translation="MNHQKYQRELMMKEKINDTEPGIKQIEREIERGCDNAKKYFWLF
                     VVFFAAGLIVRNVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLA
                     AASLSPLCEIIFGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="JNY02_001237"
     CDS             complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="JNY02_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBB9432848.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="JNY02_001238"
     CDS             complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="JNY02_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBB9432849.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="JNY02_001239"
     CDS             complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="JNY02_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBB9432850.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9819..10154
                     /locus_tag="JNY02_001240"
     CDS             9819..10154
                     /locus_tag="JNY02_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HBB9432851.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="JNY02_001241"
     CDS             complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="JNY02_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HBB9432852.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10490..11233)
                     /gene="traF"
                     /locus_tag="JNY02_001242"
     CDS             complement(10490..11233)
                     /gene="traF"
                     /locus_tag="JNY02_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBB9432853.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11226..11483)
                     /gene="trbE"
                     /locus_tag="JNY02_001243"
     CDS             complement(11226..11483)
                     /gene="trbE"
                     /locus_tag="JNY02_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBB9432854.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11510..13339)
                     /gene="traN"
                     /locus_tag="JNY02_001244"
     CDS             complement(11510..13339)
                     /gene="traN"
                     /locus_tag="JNY02_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBB9432855.1"
                     /translation="MVFFLSFSVFADSNSDYRAGSDFAHQIKGQGSSSIQGFKPQESI
                     PGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAP
                     FIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRTASITGDW
                     KYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFM
                     NTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLV
                     SGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKP
                     WNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSC
                     ESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAAGKDVAALNGVDV
                     RAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGEFC
                     SRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGITVDELQKI
                     QFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13650..14030)
                     /locus_tag="JNY02_001245"
     CDS             complement(13650..14030)
                     /locus_tag="JNY02_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432856.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="JNY02_001246"
     CDS             complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="JNY02_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBB9432857.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14674..14979)
                     /locus_tag="JNY02_001247"
     CDS             complement(14674..14979)
                     /locus_tag="JNY02_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432858.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15009..16001)
                     /locus_tag="JNY02_001248"
     CDS             complement(15009..16001)
                     /locus_tag="JNY02_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBB9432859.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15998..16630)
                     /gene="traW"
                     /locus_tag="JNY02_001249"
     CDS             complement(15998..16630)
                     /gene="traW"
                     /locus_tag="JNY02_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBB9432860.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="JNY02_001250"
     CDS             complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="JNY02_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBB9432861.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(17010..19640)
                     /gene="traC"
                     /locus_tag="JNY02_001251"
     CDS             complement(17010..19640)
                     /gene="traC"
                     /locus_tag="JNY02_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBB9432862.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(19766..20113)
                     /locus_tag="JNY02_001252"
     CDS             complement(19766..20113)
                     /locus_tag="JNY02_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432863.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(20141..20359)
                     /locus_tag="JNY02_001253"
     CDS             complement(20141..20359)
                     /locus_tag="JNY02_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432864.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(20458..20931)
                     /locus_tag="JNY02_001254"
     CDS             complement(20458..20931)
                     /locus_tag="JNY02_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432865.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(20924..21337)
                     /locus_tag="JNY02_001255"
     CDS             complement(20924..21337)
                     /locus_tag="JNY02_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432866.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(21330..21551)
                     /gene="traR"
                     /locus_tag="JNY02_001256"
     CDS             complement(21330..21551)
                     /gene="traR"
                     /locus_tag="JNY02_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBB9432867.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(21686..22201)
                     /gene="traV"
                     /locus_tag="JNY02_001257"
     CDS             complement(21686..22201)
                     /gene="traV"
                     /locus_tag="JNY02_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBB9432868.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22198..22566)
                     /locus_tag="JNY02_001258"
     CDS             complement(22198..22566)
                     /locus_tag="JNY02_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBB9432869.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINVITARVVPDKNVSDDFMHAVL
                     SNCTTKIVLPAPKVFSSESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKT
                     NLTAELISAVVGSNKKGEGQ"
     gene            complement(22505..23095)
                     /locus_tag="JNY02_001259"
     CDS             complement(22505..23095)
                     /locus_tag="JNY02_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBB9432870.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(23085..24512)
                     /locus_tag="JNY02_001260"
     CDS             complement(23085..24512)
                     /locus_tag="JNY02_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBB9432871.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(24512..25240)
                     /gene="traK"
                     /locus_tag="JNY02_001261"
     CDS             complement(24512..25240)
                     /gene="traK"
                     /locus_tag="JNY02_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBB9432872.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(25227..25793)
                     /gene="traE"
                     /locus_tag="JNY02_001262"
     CDS             complement(25227..25793)
                     /gene="traE"
                     /locus_tag="JNY02_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBB9432873.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(25815..26126)
                     /gene="traL"
                     /locus_tag="JNY02_001263"
     CDS             complement(25815..26126)
                     /gene="traL"
                     /locus_tag="JNY02_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBB9432874.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(26141..26500)
                     /gene="traA"
                     /locus_tag="JNY02_001264"
     CDS             complement(26141..26500)
                     /gene="traA"
                     /locus_tag="JNY02_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBB9432875.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(26534..26773)
                     /gene="traY"
                     /locus_tag="JNY02_001265"
     CDS             complement(26534..26773)
                     /gene="traY"
                     /locus_tag="JNY02_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HBB9432876.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(26881..27528)
                     /locus_tag="JNY02_001266"
     CDS             complement(26881..27528)
                     /locus_tag="JNY02_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HBB9432877.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(27720..28103)
                     /locus_tag="JNY02_001267"
     CDS             complement(27720..28103)
                     /locus_tag="JNY02_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBB9432878.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            28436..29026
                     /locus_tag="JNY02_001268"
     CDS             28436..29026
                     /locus_tag="JNY02_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBB9432879.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(29323..30144)
                     /locus_tag="JNY02_001269"
     CDS             complement(29323..30144)
                     /locus_tag="JNY02_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBB9432880.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(30255..30551)
                     /locus_tag="JNY02_001270"
     CDS             complement(30255..30551)
                     /locus_tag="JNY02_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432881.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            30851..31228
                     /locus_tag="JNY02_001271"
                     /pseudo
     CDS             30851..31228
                     /locus_tag="JNY02_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31466..31624)
                     /locus_tag="JNY02_001272"
     CDS             complement(31466..31624)
                     /locus_tag="JNY02_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBB9432882.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            complement(31904..32623)
                     /locus_tag="JNY02_001273"
     CDS             complement(31904..32623)
                     /locus_tag="JNY02_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBB9432883.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(32620..33054)
                     /gene="psiB"
                     /locus_tag="JNY02_001274"
     CDS             complement(32620..33054)
                     /gene="psiB"
                     /locus_tag="JNY02_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBB9432884.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(33109..35067)
                     /locus_tag="JNY02_001275"
     CDS             complement(33109..35067)
                     /locus_tag="JNY02_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBB9432885.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(35131..35364)
                     /locus_tag="JNY02_001276"
     CDS             complement(35131..35364)
                     /locus_tag="JNY02_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBB9432886.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(35420..35941)
                     /locus_tag="JNY02_001277"
     CDS             complement(35420..35941)
                     /locus_tag="JNY02_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBB9432887.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            complement(36763..37326)
                     /locus_tag="JNY02_001278"
     CDS             complement(36763..37326)
                     /locus_tag="JNY02_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB9432888.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(37373..38734)
                     /locus_tag="JNY02_001279"
     CDS             complement(37373..38734)
                     /locus_tag="JNY02_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBB9432889.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(38786..39016)
                     /locus_tag="JNY02_001280"
     CDS             complement(38786..39016)
                     /locus_tag="JNY02_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432890.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(40054..40245)
                     /locus_tag="JNY02_001281"
     CDS             complement(40054..40245)
                     /locus_tag="JNY02_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432891.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(40242..40664)
                     /locus_tag="JNY02_001282"
     CDS             complement(40242..40664)
                     /locus_tag="JNY02_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBB9432892.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(40711..41136)
                     /locus_tag="JNY02_001283"
     CDS             complement(40711..41136)
                     /locus_tag="JNY02_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBB9432893.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(41554..42330)
                     /locus_tag="JNY02_001284"
     CDS             complement(41554..42330)
                     /locus_tag="JNY02_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432894.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(42381..42815)
                     /locus_tag="JNY02_001285"
     CDS             complement(42381..42815)
                     /locus_tag="JNY02_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBB9432895.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(42829..43050)
                     /locus_tag="JNY02_001286"
     CDS             complement(42829..43050)
                     /locus_tag="JNY02_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432896.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(43051..43734)
                     /locus_tag="JNY02_001287"
     CDS             complement(43051..43734)
                     /locus_tag="JNY02_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBB9432897.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(43811..44104)
                     /locus_tag="JNY02_001288"
     CDS             complement(43811..44104)
                     /locus_tag="JNY02_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432898.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            44252..45214
                     /gene="parM/stbA"
                     /locus_tag="JNY02_001289"
     CDS             44252..45214
                     /gene="parM/stbA"
                     /locus_tag="JNY02_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBB9432899.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            45217..45567
                     /locus_tag="JNY02_001290"
     CDS             45217..45567
                     /locus_tag="JNY02_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBB9432900.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(45690..45971)
                     /locus_tag="JNY02_001291"
     CDS             complement(45690..45971)
                     /locus_tag="JNY02_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432901.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(46099..46333)
                     /locus_tag="JNY02_001292"
                     /pseudo
     CDS             complement(46099..46333)
                     /locus_tag="JNY02_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(47269..>47765)
                     /locus_tag="JNY02_001293"
     CDS             complement(47269..>47765)
                     /locus_tag="JNY02_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432902.1"
                     /translation="QGLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSD
                     TKATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKNIDNYFLFIKQKIESFNFNEAL
                     KQQILKDISLCSYEGLHVLKIEIKSVGEVCHFENRYYIRQGTATHLITDGEAISSLHR
                     LYYS"
BASE COUNT        11690 a        12957 c        11283 g        11835 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccca caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctgcatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcatttttt ttgttttcat ccagaatatc cttttaacta acagactctt
     3601 ttttgaacag acctattact gagactattg cagagagatt ataacgattt atatctatag
     3661 tataaataaa ggaaataatt acaaacagag ttattgataa aaacccaaat aagaacgtat
     3721 gagcaaagga aactattgaa aaaacaatca caaacgacaa gatataagtt ttgttcttat
     3781 catacataaa agatataact ttcgactcat ctctgaattt tttagggatg gcaagataca
     3841 acatcattcc attagtaatg gcaacaaaaa ggataaaacc cattacgcca ctaaaacata
     3901 tatcaaatcc agcatctcgg atatcaactt tatctgccag caaacgaaga aatgtcggcc
     3961 ataataaaac ccataaaaac acatacaccc ctggttttat gacttgtaat aatggcggaa
     4021 tattaactcc ctctacagag agtacatcct ttagtttctg tgtctccgtt tttatatcat
     4081 agtgagtgat cataatggcc ccttcagtct atccttttta tcatcaacca tttttttcac
     4141 agcctcttct gatggctcag gtattatctt ttgactttct cgctcttcat tatatttatt
     4201 attctgagat aatgcctctg ttttatgatg attttgcagt tcagaatact gccctcttac
     4261 aatattttcc tcgccacgga tactgttctg agtatctccg atattgccac gatattctgt
     4321 gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct gcgttctttg
     4381 ctcaataatg gcctgatgtc cctgatggtc agcaataata tcctgactgc caccacccga
     4441 aggtacgctc tccattcctt taccaatatc gccacgggat tcccgccagg cattatcaac
     4501 accaggctgt acctgttcct gcacaaaaaa ccaggccatc gcccgacgac gttctgcaat
     4561 ctccggcgaa ctggtatttg tcagaatagc ttctgcatcc tgcggtgcgc gtttcatcac
     4621 atactgtgca aactgttgcg acaggtcttc actcatctgc ccgctcatgc tttcagtacg
     4681 ggaggccatt tccgcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
     4741 ctgtttcgct gagttcaggg ctgcagacaa ctggtccacc cgggagtcag cattattgtc
     4801 agtatggctg ccggactcac tgaccttgcg actggtaaag taatcgctgg cttctttaaa
     4861 gtccttactg gctttagcgt caatatcatg gcgagcatca tgggtagctc tgctgccact
     4921 acttgcagaa tgtgaatcat catccgttcg atcaagactt cccttaaacc cgcctctgac
     4981 accaacccca ccaccaacac caagaattcc aggtttaatc ccccattcag catgagcatc
     5041 tgcatatccg ccatacgaga acttagaacc tgttgatgcc agttcctgag tcgcctgctc
     5101 attactgata ttatgcgcct tcgcatagct ttccactgca ctgcgcatac ggctggccat
     5161 catggagtcc tgtgcactca tggtgctgtc agcgccaccg gtgacagaat cactgctccc
     5221 ccggttagaa ccaaactgac tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
     5281 atgcagggca ctttctgctc tgttcgacgc ctcccgggcc atttcctgtt gcgccgctgc
     5341 aatctgacgc gttgcattga taccgaccgg taaacgggac atcgctccgc ttgcatccat
     5401 caccatatta ccgtcacggg tctgtgtggc agttgcgcca ctgccggtct ggtacatcat
     5461 ctgaccaaac gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tttccgtctg
     5521 catgttgccg taggagtaat taccgtcaac cacactgcct gctgcactgg ccgtcggact
     5581 gatagaggaa gaggcgaagt ggctgtacac gctggaaaaa cctgcgccaa ggccttttac
     5641 catcatccat gacaacgggg ggatcatggc tgaaagatac ccggcagtag aggccaggtc
     5701 agagtatttc agctgtatct gagagagttc agacagcacg accggcgcac cattcatctt
     5761 cgcatagaat gtcatggcac tgttcagaat ggcatacaac aacggccagg tctgcagcca
     5821 catcagggca aacacatagc ctttcaggac ggacagtgtc agtttgttga agacagctgc
     5881 cagtaccaga agcgggaaaa taccaatggc aattcccgtc aggatagtct gtaccattgg
     5941 caggtttcgc attgtcacat gaccgataga gacatgcgcc agacgttgct tctccatcga
     6001 tgacgtggtg gccagattca ccaggctggc ggtgtcacca ttacgggcag cattactggt
     6061 gataccctct ttcagggcat ttattgtgac gttctggcgc ataatctggc tggcagactg
     6121 actggagccg tagaaataac tgtaactgtc actgaccagt tgtctgaata acaggtccgg
     6181 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctcccgtctt
     6241 tgtatcgaga ttcagcctgt ctttcagcgt gaccgacgca tccttacagg ttatgaaatt
     6301 attattgttg tcataaacgc cccgtagcgg gctgggtcgg gaaaaaatca gcgtgtaggg
     6361 gtcagcggag gccatcagat cttccagcgt gtatttatga ttcaggtaaa tatcacccag
     6421 cacgcagttc tggacgtagt cctggaacag attgatgatt tccggattcc gggacaggaa
     6481 atcggtgctt ttcacaatca gattcgcccc gaacagcatg cctgtcttgc tgtaggtgac
     6541 gctgtccggt tgggtgaata tcatctcgta gctgaccacc atcgcatgcc cgatacgggt
     6601 cgtaagagaa agtggcatcg ccagaccgac cggaacatta tccacccgat ggactttgac
     6661 caggtcactg ttatcaataa tctgcaccga tgtgcggaca ttaaccagca ggctgataag
     6721 cacaaacacg gccacccagc ccagcaaatc catcacgtta tgccgctgta cccacataaa
     6781 agccacagca acaacagaca gggtgagcgc gattttttca attgaatccc aggtccgggt
     6841 actcataaat gcagcaatgg cgttcaggtt attccgcaac cactcaccac cggcaatcac
     6901 ataaacttca ttcacagtga attccctccg aagtgatagt tgttctggta acggctgagc
     6961 atacgggcgg aaagctgctg acgcatgtag ttcatctgac gttcgacaac cagcagcgca
     7021 tcctgctgga cctgtacctg actctggaaa atggcaatct gttgctgtgc ctgattcaga
     7081 ttctccagaa cattgtccat cacagcctgc ggatagttcc cggtggcaat catggcccgg
     7141 gcctgctgaa gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc
     7201 agctggtaaa cgacgctgtt ggacacaccg agcatctgcg ggtcaatcag atatttaaaa
     7261 acgggaatgg tggtgctgga aataaacccc tgttcccggg cgtcaagcgg agtgtcactc
     7321 actgctttat tctgaatact ggtgagcagt tttgtaatct gtgacttcag ggctttatcc
     7381 cgggcgatgg tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag
     7441 tggtaaattt ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta
     7501 cgcgccggca ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc
     7561 atgataaatt ctttcaactc tttgttgccg tcaaacattc tgtttttttt cagcgcctcc
     7621 catatgatat tctggttttt cagcacccgc tccttgtcct tgtcgctggc tttgtcacga
     7681 acagatgagg actgccctcc gaccgtgcac ccctgacgag atgcggccca gtcggcgaag
     7741 atattgcttt cactgccaat gtcctgacaa actttctgct gagattcctg cgtctgaggg
     7801 aacagccccc ccacaagccc ctgtgccacc tgacaggaac tgaggttcat actgttaatg
     7861 tcacttgcca ttttctgcag gaagtctttt gcggttttta tttccggcac cgttgtctgc
     7921 agggcaaggt caaaaaagta accggcagca ttgctcataa tctgcttaac aaaacgctgc
     7981 aactgatcgc cattaataaa actgaacgag ccgagatagg cgtcgatgcc gccacatccg
     8041 gcattgatat ccggcagcgt catggaaatc agctggatat ttttgacctg ggtgcgggca
     8101 tacagggagc caccataggc atacccggcg gcctgcccct gccagacgcc cggctgtgtg
     8161 gtattggagg cgaagcccag cttgttaaag aactgattca tatcgctgtt cacatccgct
     8221 gacgctgcgg gtgtgaccgt cagtaaagcg gcacaaagaa ccagaagagg tttaatgcga
     8281 agcattattg ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa
     8341 tatccggaca cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa
     8401 accagctgca agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata
     8461 acgaaaatta tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc
     8521 atgcataacg ttccttacaa tcaatccggc agcaaagaaa accacaaaaa gccagaagta
     8581 tttttttgca ttatcacaac cacgttcaat ctccctttca atttgtttga ttcccggttc
     8641 tgtatcattt attttttctt tcatcataag ttccctctga tatttctgat gattcatgca
     8701 gacaggtcat gcggctgtaa tatatttcag acaccgaaat ccatcccaca attgccccca
     8761 caaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc
     8821 tggcgggata aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg
     8881 tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc acctttttcc
     8941 tctccgtata tctgcagaac ggtatccatc cgcgccataa aactggcggc atccgttgcc
     9001 ccctgcaaaa gcggtaatgc ctcaagtgtg ttgacgttga ccaggaaggt ggtcggtgtg
     9061 gccaccggga tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc
     9121 ggaaaggcag tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc
     9181 tgtgccagct gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata
     9241 aacagcacca ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag
     9301 cggggagccg gcttttctgt tctggctgat gtgtctgctg ccggttgtgc aggctgcacg
     9361 gccattccct gcggattcca gagtcgttca atttcatccc gggtggaggc ctgcacacct
     9421 gtggccatca tcaacaaaat gaccggcagt aatttagtga gagacatgtc cgccctcctg
     9481 ctgcccttcc atcatggcct gttgcctggc ttccagtgcc tcacgctctt ccaggctgac
     9541 acgtgcaatc catgcatcca gcacctggta tgcgccgaac agcacaagga tggccgcaat
     9601 caactccgcc agaaaaaaag ccatccaggg tttgctgtaa acaagacggg cgacgatatg
     9661 aaaccagaca cccagggaaa atacccacat tcccgtaata tcaagccggg gtaaagagaa
     9721 tctgcgttta cttatcatgt taaactccgg ttattattta catgagagta ttcatgtctg
     9781 taatattcgc gtttgtgtga tattaatcag aggtacaaat gaaaaagtta tcttttaaag
     9841 aaaaactgga aatagtgaga aacatcataa gagagtcatt atcaggtaat gcggcttttg
     9901 ctttactggt gtattcatta ttgcactccc tccccattaa aacctttgct gatttatttg
     9961 tgacgatact gttatttata gcgattatcg ctgttgtgat atggctattt ctaatcatct
    10021 atatttattt ttgtgagtta ttcagaagtc actggatagc agtctggttt atcctctggt
    10081 ctgtggtaat caacctcact attggatttg gcatttatga caaattagca ccagtcattt
    10141 cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca acataataca
    10201 aacaaacatc cttctgaaac tgtcaaactt cgtgataacc ggtatggtac tgactgccat
    10261 tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca agaaattatc
    10321 tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc tcagcagata
    10381 aactccttta ttatttggtc ctgtaaagaa ataaccaaca acccccacga taaacaggct
    10441 ggcacctgta aaaaaacaca aatcccctgt atgcatatat aacctttggt taaaaattcg
    10501 gtttaaaatc ttcagaaacg ttcaggaact gttttgccag gtcgtcctgt gaaataaagc
    10561 cgtatgataa cgggcgaaca ctgccctgtt tcgggtcaac cagcatcatg gccgggaaat
    10621 atttcacacc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc aacagcggat
    10681 taatcacgcc atccacggaa acgggaataa cactcagacc atacgtatcc cggaagccat
    10741 taatgacctg cgccagttgc ccgtcgatgg ggtcctgccc ccggtaaaaa aacatgatgc
    10801 cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct gccgccagct
    10861 ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt tcgggatgtg
    10921 ccagcatggc ctttttagca ctcatggtga aaagcccggc ctgctgagtc cagtaattct
    10981 gcagccggaa atatttaaca aaattatcca cgccgggata cagaatggct tcgtacagcg
    11041 cccgctttgt tgccgtctgc agcgcggcca gtttctgcat aatatccggc tcctgacggg
    11101 gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg ttataccact
    11161 gccagcctgc atctttccct gacgccggaa aaataaagca gcagagtaac agtggcagta
    11221 atgctttatt catcttcatt cctttcatca ttttttcttt catcatcttt ctgcaggcca
    11281 gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta aaggataacg
    11341 aaagcagcag caagtatccc gtctgccgta gtttctttta ccgtatccca cgaccagata
    11401 attaccggag aaatgacaat cgctgtaatc gaaagccgga taagaaaatc aacaagacga
    11461 ttagaagtga atataacttt catcatttcc cccggttatt ttccatacct tactgtcctg
    11521 cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg ccactgtccg
    11581 ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt ctgtcgaact
    11641 gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc gcactgccaa
    11701 acccgatacg caactgcccg ttacgcccct gctgctggac tatctgcgcc agcttcgaat
    11761 caaactggca gtagctgcgc tttttctgca gacagacacc cagcactttc cgggagcaga
    11821 actcgcccac actcaccgtc agtttgttgt cctttgcctt tcccagcgcc ttttcttcac
    11881 tgttgcactt cgccagcccc acatcctgac cccagccgcc atccttacag cagttactga
    11941 agccggctgc ggccttacgg cagaatttcg cctcacccgt gaaagccctg acatccacac
    12001 cattcagggc ggcaacatcc ttacctgcgg cggcaagcgc agccagctgt gatactgctt
    12061 caccaaaatc actgtttttt ccgctctgtg ccttatcgca ttcaccgtca aggcagaaga
    12121 catcaccacc acagaccatc acttttcctg atgttctgga ctcgcaggag tacgtggcgt
    12181 actcatgcag acaggttccc tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac
    12241 aggccggatt atcgacatat ttctgacagg tgccgttatc tgcggactga gtgacatatt
    12301 tatcccggta tgcccagcat gcctgtgtga tgttccaggg tttaccttcc atcactccgg
    12361 ttttcgtacc acccggctct gaacactcag tgcctgtcag cacgccatcc gctttattaa
    12421 acggacaact ctccacccac tccacccggg gaacccattc tttatcacgg accttcatcc
    12481 tgagtttcag cgtaaaggtg gaagcaccac tgacaagcga ttcatagact ttcctgtcac
    12541 cattcccgtg cgggaggcaa ttaccgttag cagtacagcc actaccggtc agaacctgcc
    12601 cctgtgtcac ctgcaaacct gtcgccccgg acagggggta agtatccgac tgcccgtttg
    12661 ccaggccgac attaaaggtt gtattcatga aggtataacg ggagttcagg aagaaaaaag
    12721 cggcgtatac agacaatgat gcactttcca ctgtaccggt taccggagcc gtgacactga
    12781 acaccagttt cagcccgttc atactgaacc ggaactgtga gtgcggtatc gtcacttcac
    12841 gatattcatc cgtgtatttc cagtccccgg tgatactggc agtccgtgtg cagtactcct
    12901 ccaccatcgt gtcccgctca caggtatagt ttgtgaattc actgcggttt atctcctgcg
    12961 cactgcactg ctgtccggta tttcccacaa tgctgtcagc cctgttcacc acatccctgc
    13021 cggtctgaat gaacggtgca tccggtgaca gaatgtcttt cggcttgttc atgaaggact
    13081 ctgtgatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    13141 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    13201 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    13261 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagca aacacagaaa
    13321 agctcagaaa aaaaacaatt gcacaaagat tgcacggctt tacggaataa ttaaaattca
    13381 gagatctgaa aaaggtattt atcttttctt tcataacctg aaccatactg gagaatgaag
    13441 tgatgtcatc tcaacttcag aactgtgttg attgtggtaa agcattaagt cgaacagcca
    13501 caacgtgcgg gaattgcagc tcaacggacc cttttggaaa aaaacggcgc agcgatcgct
    13561 tttaccagtg gtttgtaggg ctgtttatta cggctattat tattgctggc ggattatggt
    13621 acatggggct tcttgacgta ccgggattac tactcagata acgacttcag taacgatatc
    13681 agtgttctga aatcggcaca taacgagtga cgacaatacc ggttatacca gtaatgaggg
    13741 gtgtcgggct gaagcctgaa acagagctga aacttactgt taaaagcccc cttcaccgac
    13801 catccccatg ccagacgtgg tattgctgat tcagcatcct gccgcagtcg ctgctcaaga
    13861 agggcagcca tttttaccgg acaggcaaag gcaatataag gaggcagctt tgtaacaggg
    13921 aaaccatgtc cctgacagga agcatatgtg cggaagccgt gaatgttcat cgcggccacc
    13981 agggcacgga tctccggatc aatctgataa tacccggcag gtaaagtcat ttttgtttat
    14041 ctcccgggtt attcagcaac gcccgagcca cctgccggca gtcccccgtg gcggccactt
    14101 tctccagcgc ctggccgacc cgcaggttac ctcggataat gtcgtatccc tgactgcaga
    14161 acaccaccag tgccggtaca ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct
    14221 gaacaccatc agtcgcgccg tctttcacca gggacagcac ggcttcagcg gtggtcttca
    14281 ggtcattatt caccatgccc cgcagtgtgg cgggtatacc gtagtgccgg gtttcaccca
    14341 gcatccgttt cagcccctct tcgggaatgg aaaacgacac aaaatacagg gcaccctgcc
    14401 ggggtttccc gtcctgcgaa gcctgctgtt tacgaaccag ctcatccaga aaatggttat
    14461 ctgaacgctg aagggggttt tccagcacct gtttctccgc ccaggctttc agctgatggt
    14521 caggtttctc tcgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt
    14581 caggtgtgtt cacattttct gaagcatgaa cagcaagatg catccctgct gtcagagcaa
    14641 aaagtgcaaa tattttccgg ttcatgatat ttctcacttt ctgctattta caggcatgaa
    14701 ctcatcagaa aaaagataat tgagaaaaac agccataaac gcacatgtaa atgtcacctt
    14761 cagatataat ctgacaagtg tcacttgttc tgatatatct gccttcttcc attcatgaat
    14821 aatttcagcg agaaaactgt caggtgcaaa ataaaataat ataacgggca ccattacaag
    14881 accaaaagga aataatttta atgttctgct tatcagcctg agcagcacag cacttatttt
    14941 cctgaggtgc tgaatatatt tactgtccat tgttgccatt ttcatttcct cttattcatt
    15001 aaaatgattt acaggaatac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
    15061 tttgtgttgg gcggattttt tcccgtctcc cagcgcatca cactgcgccc gaacggatgg
    15121 cactgcccgc tgtccggata catattcacc atctgataac gccagcgttc tttgggcagg
    15181 attggggacg gatattcatt acagacggcg ttatttttcc cgatggtttc cataatcatg
    15241 ccctgacggt gtagtttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
    15301 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
    15361 cagaacagaa catcgagagg catattaaag gcgctggcaa tggcatctgc tgcacaggcc
    15421 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
    15481 ctgtccgtcc aggtggggtc aatttcagaa agataagcga tatccaggtc accaccttcc
    15541 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
    15601 acgtgataga acgccccgtt cacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
    15661 ttaccaaaag ccggcaggct gaagcccagg ttaaccatgc atcccggtga ccgggtgacg
    15721 tccgtcagcg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
    15781 ggcggtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttaccc
    15841 tgactgactt tgacactgcc cagcgagagc gggaaaatac agctccagca gatatccgtg
    15901 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgctgcagg gacatgaccg
    15961 gcgagaagga ataacatcag cagccacagg cttcgcttca ttttctgccc tcctctgccg
    16021 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
    16081 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
    16141 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
    16201 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
    16261 gcgtctggtt aaaaggcaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
    16321 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
    16381 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
    16441 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
    16501 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatcgcccc
    16561 aggtgccaag atcggcggcg gccacactct gcccccatat cagcagggca atcagccccc
    16621 ggcatctcat ggttccgctc tcattcgccg ggcaatatcc tgctggattt cccgggtgat
    16681 atccggagcc ccctgtacca cggcaggcga caccagaatg accgcgtggt gtttctgctg
    16741 ccacgcctgc aggctggctt ccagtgccgt gttaaaacgc gctgaaaggg cttttgactg
    16801 tgcttctgac agttgtttct gactggcgct gtcaaaaaat gcatccacag tctgtttcat
    16861 gttgaaggcg gcagtcaccg gtgcgttcag tctgacaatg ccgtaactga cggctgcgtt
    16921 cagtaacacc attgccaggc atcccggcac cgtccaccac cagtggctgc gtcggggggc
    16981 tgtaacatca gtcgtttttt gcgttgtggt catgcaacac tcctgtattt ctcatgttct
    17041 tccagccagg cttccagcga agccatctcc ggccctgact tcttccacgc cagctgccac
    17101 accgcctcat gaatactcag gccctccctg cgtttctgct gcacaaactc aaaatccggg
    17161 ccgtcagagc tgtacatggc gcggcttaac gggtccacaa acagacggtg ccaggaggaa
    17221 tggttttcca cctgcagcag gaaggaactg aaccactggt ctttggccgc accaaacttg
    17281 ccaatcatgt cgcgctgcag tggctggaac tgatccggga ataactggtt atatttcgcg
    17341 aactccttcg cactctgttt gaggataatt ttgtaggagg agttacccca tgccgcacgg
    17401 gccgcactgg aggccttgtc agagtcaaag tcgacgatgt tctgtgtgat ggtgatatag
    17461 gcaccggtat ggcggcgaca ggtgcggtaa cctttttgaa taaactcacc gaccttgcga
    17521 tttttgaagt ccagcagacg ccagccttca tcaatgacgt tcagtttctt gagagtacgc
    17581 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
    17641 agtgacggac ggtcttccag tccgcccagc tccagcacca ccattttcgc atcatcccgc
    17701 agggacggct catcggagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
    17761 agcagcacaa tcatttcgtc cagacggctg cggatggtcg gtgacccggc atactgctcg
    17821 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
    17881 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
    17941 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
    18001 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgcccccc
    18061 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
    18121 gagtccagca cgctgcggat aagtggctgt atcagtccgg ttttaccggc cccggaggtg
    18181 ccacagaccg ccatgttgta gttggtgtta ttcatcccct taaagaagat atcgataaac
    18241 gccagctggt tacggtaggt gggcgccagc agacctgccg gtgtcagggg gttatccgcc
    18301 actaacggca tcaggttggc cacattaaag ctctctgcgc gctgaaccac accggcctct
    18361 ttcagctgtt taaacagccc tttcccggcc ataaagggca ggcaggtcag gaaattgcgc
    18421 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacggaagct gttcaggatg
    18481 tcctgctcca cttccagtgc cgtttcatta ttgtccttgc agaaggctgt gatgttgagg
    18541 aagtaggaca cgacagaaga ctggccggag cccagccgct ggcgcagttc cccccactcc
    18601 ttcgcctctt tctccacgga cggaaaccat ttggcatatg aggtcttcga cttcttctcc
    18661 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
    18721 gtcagcgtca ggatgaacgg gcaggaaatg gacatttccg ggttcagcag attgctgtag
    18781 ttgtcggcca tgttccacag gaaggcgatt tccgggttac gggccagatg gaaattcagg
    18841 atgcgggccg tgctgttcct gccgttctca cgcaggccca gcgtcaggta atcagcccgg
    18901 acattcaggt caaaactgtc ctccacacac tgataattca gatcagaata cgggtccagc
    18961 tgacgtcttt tcgggtacag agagtccgga ttatggttaa tcatctcccc gacaatctcg
    19021 atgaaagcct gtgcatccac tgtctgtgtg gtgatttttg cgccgtgaaa agaggcacgg
    19081 atgattttca ccagattttc catttccaga atgtcagccc ggcttttttt cttcgaggga
    19141 gaacagtacg agataaacac ccggtaatga cgcagggtca ggggcagatt catcccctcc
    19201 ggcagcggga actgtgtcgc tgccgctttc atataatagg cacgggtaat ggcgttaaac
    19261 cgttcagcct gttcacctga ccaggagaac tcacgcagcc cgtattcaat cctgtcacca
    19321 accagctgac tggacatcag atggatacac aacggaatac cgcgcggcag tttggtgcgc
    19381 agcatgtgat cgagtgcctc cacaatggac ttattcgccc cattgatggg aatggcttcc
    19441 agcataaagc ccatcgtggt gtcattcatg aacagaccgg attcctggtt gtaatcacgg
    19501 tacggcagca gacggctgaa ctgcgggaag ctcatttcgc ccagaacttc attggccttt
    19561 gcggattcgt caggcagttt cagtgctgtg acgagggagt taaccgcctg agtgacggcc
    19621 tcaagtgggt tattactcat atgagtattc cttagtgcgt ttaaaaaagg aaaatatcag
    19681 tctgtggccg tggatatatc acgcagtgcc attcctgaaa ccacagcaca aataagagat
    19741 ggaggctttt aacaggaaac caggattaac gttcctttac tgaatgagca tcatttaatg
    19801 cctgaatttg tttttcgcga atcttattgc gctcattttc tcttacccgt ttcacctctg
    19861 ccatttcgtt tctttttctg cattctgatg cgattttatt tgccttacca agtgttaaag
    19921 gttcagactg ataattaagt gtctcctgaa atgcttctgc tgcacaggga gtttcctgta
    19981 aaatctctgt cagttgttct ggtgttgcag gttgttgcgc aaggtaaacg atgtaaaaag
    20041 gaatacatac aatcatagcc ataacgccaa caaatataaa agccagaaca gattttaaat
    20101 atatcgactt catttcattt tccttcgaag cagtaagcac tcattgccca ccaccattta
    20161 gtgtttccat cacgctgttg aaaggttcat ttgttttaac ttcaatacac tcccctgtat
    20221 ttccaaagcc cctgccgcca tgtgcttcga ttgtccgaag aacctgcacg catacgtccg
    20281 tatttggcat gggtgtaata ttccagcttc cgccaccgtt aatatttttt atcagcacaa
    20341 gaaaaataaa ggtatgcatt ctttattatc tccgcttgta tattaatcac tgaatatccc
    20401 ttcagttaac ttatgaggtc ataaaacact gcagattttc attcagattt actctcttca
    20461 gcaaacttta ctctcttcag caaacttccg gatggtatcc tgttcacgct gacgggtggc
    20521 agactcctgc attgcatcca ctttctcctg ccagagacgc cggatatcct tttcatcccg
    20581 accagtcagt tttttaccgg acagcagacg gttaagcagt tcctgtctcg catccggtac
    20641 gtcttcagtc agacgcataa gctgaagcag gctctgacgg ctgagaacca tgagtgattc
    20701 cggcaggagg gtgaagaaga aaataaaaaa gcataccgca ccacagcagg caaggaccgc
    20761 aaacaggatt ttcagatata taccatcaac gccgaacgca gcaataatac agggaatcca
    20821 tgcaatacac gcacaatacg ccaggatgtt atctattcgg ttatgtctgc gatatatctg
    20881 ttgatgttca tccagaatag aatctatttt ttcagcttct gatttatgca tagtgtttcc
    20941 tctgcgtttc ctgccagaga cgccggatat cctgttcatc ccgaccagtc agttttttac
    21001 cggacaacaa acggttaagc agttcctgtc tcacatctgg tacgtcttca gtcagacgca
    21061 taagccggag taattcctga cgactgagta ccatgcgtga ttcctccggt acgctgtaat
    21121 aattaaaaat acgactgaat accccacaac agcaggcaac ggacaggcac agggatttca
    21181 gatacaggcc agaaacacag aatgtggcaa caccacaaag aatagctata actaaaaatc
    21241 ccttacccag tgccatacac aaagaaccct gttttctgga tatccgctca tgctcatcca
    21301 gaataaaatc tattttttca ttttctgatt tatgcatagt gtttcctctg cctttcctga
    21361 tacgcctgac attcaacgca caacgtcaca ccgggaaata ttttccgccg ggcttccgga
    21421 ataggatttc cgcatgcttc acagagataa acaggaatac catgaacatt tatttcctgg
    21481 cgtatccggt ttattcctgt catggtcagt tgttctgtca ctgaatatgc ttcatcggct
    21541 tcatcactca cggaatattc ctcccgacat gttctgacac gctaccgccc atcccttttc
    21601 acgcacaggg cactcgtttt acaggcggat tttgaataca gaaagcccgc cggcattgcc
    21661 ggctgatata caacttcagg gccagttaat taatacgtgg ttttccccac gcagacggtt
    21721 taataacaaa aaacacgctg gatggctgat gataaacatc ctggttatca atataaggcg
    21781 ctatccataa tgctgccgtc tgctcacccg ttcttagcgg acgaggaggt gttaccggcg
    21841 taactgaact gaccggaaca accgttttca cttcccgggc agcggtaaac agcgggcgtg
    21901 gtgccagcag tttctgctcc ggatgtgctg tcaccgcagg tctgctgccg gacggagtgg
    21961 tcgcggtgac ggtctgcacc ggcattgtcc ggaagttccc ttcagccagg cgcggcagtg
    22021 atgccgcaac cggctttgct tctgaggact gctccagttt tttggccttc tcattggcct
    22081 gctccatcgt catacaggta tcggatgtgg tggcgttaca ctcaaattcc gtatttgttc
    22141 ctgcgcagcc ggaaagtaat aaggttccca gcagaggaat aaataaagaa atctgtttca
    22201 ttgcccttct ccttttttat tactccccac aacagcagaa attaactctg ctgtcagatt
    22261 tgttttccct gcacagggat gtccggagac cactgtattt cccggagaag aaaataagct
    22321 catcgacatt cccttcttca tcacaccgac agcagccata ttaaatttgt gcggcaatga
    22381 ttcagaactg aacaccttcg gagcaggaag aactattttt gtggtgcagt tactgagaac
    22441 agcatgcata aaatcatctg atacgttttt atccggcaca acgcgggctg taataacatt
    22501 aatattacgc atgttcattt ttatctccgt tgcttctgtt tgttcggtca tcagggaaat
    22561 aaacatggca tttcctgatt tttcccggca gggaagcgtc ttccgtataa acgtccttca
    22621 gatattcatc tgcggttacc acccggatac atttcatttt tccgggcagc accggatatt
    22681 tcccggtgat cgctgccagc gagacataca tcagtgccag tgttgcccat aatgcggtga
    22741 ccagttgcat cagtgaccgg tcagtctccc tgacataatc tgcagcatca gccgcgcggt
    22801 aatggcagat atcctgtatt tctgatctgc ctgaagaatc agaggaagaa aaccagcatt
    22861 cactgaccgg gaactgtccg gcaggtgctg tttgtcttac gaagttaatc tccttaacca
    22921 gtaatttccc cggcgtggtg ttgtccttcc ataaaacaaa cagagccatc agggccattg
    22981 tggccagtgg cagaatgacg gtgtacttca gacaccagaa cagtcctcgc agaacccggg
    23041 cgacaacata ccgggcggca tggcacgcct gacgggacga catattattt gccatcgttg
    23101 cctcctccct ggctgccgga cataaactgc tgcggattca ggttgcccag ctgcttcagc
    23161 atctggtcag tattaaagcc gttaagatga ctttcagctg atggcggaac aggaaccgga
    23221 ctttccggat tatcttcttc cgctctgttc tgcgtgcgct ccagcgccat ctcttccacg
    23281 gttttcagct ggaagccgtc ctggaacacc acggtcactt cgttgcccgc accaatcggt
    23341 atcaccgggt gatactgttc ggcacgtttg atgtagtagt cactgagcgt ctgtgcggct
    23401 ttcgatgtgc cgccaccgat acccattttc aggacatcac cagccccgta agcggctgtg
    23461 gcacccagcc cgacagccgg ctgggaggcg cgctccattc cctgaccgat accgtcaaca
    23521 aatcccgcgc cccatgccca gccgaggatt ttgccgttac gcatcaccac ttcgcccttg
    23581 ataccgtttt taccccggaa gctgacatgc cccttaatcg gcatatcaat agttttgccg
    23641 tccttcaggc aactgatatt gcgggtgcgt acaatggcac gctcactgga cacatccccc
    23701 caggcttcca gccccacaaa acatcccgtt gcgtcatacg tcttgctgtt cggcatttcc
    23761 accaggccgg tgatacgcaa ctgcatcggc accgtggatt cattaccggt gactgaggca
    23821 ttggcatccg ccccttcaat cagcatggct ttcgcaaaac ttcctgacgg aatgtacggc
    23881 agcgatggtc cctgttttcc ctcattacgg gtaaacacct tacgctgtat ccggttaggc
    23941 accggcacag actggtacgt cacctgtggt ggtggcgtga caccattccc gggataaaac
    24001 gccgtcggcg gtggaacagc aacgctgccc tgcggcggga aggatacggg ggtgtttcct
    24061 ggctgtggtt cgccttccgg cccgggcggt gaggcaggca tctgaggtac aggctcaccg
    24121 gtcgccgtga cgggattagc acccagggct tttacctgct ctgccagggc ggcattgtcc
    24181 tgtcccagct tttcaatacg acgctggtca tcaccgcgct gtttgttcag gacatccagt
    24241 tcacgacgga tttcctcata ctgcttctgc atctgcgctg ccgtcacctg catctctgtg
    24301 gtggcatgct gacgcacctt gtcatcaaag gtcgtatcca ccacacctgt catatccggc
    24361 acaggctcct gttcagccac cgcctcaccg ttaccggaca tgtccacatc agacagatac
    24421 agtaccccac caatcgcgga ggctgcaccg acaaccacaa tccccagcca caggtactgc
    24481 ttgcgtttca caatggtatt gatactggcc atcagttgcc ctccccgtca cggatgacat
    24541 acacatccat gcgcgcacct gccagtaact ggctggcagg ctggctgaac atcacggcac
    24601 gggttcccgg ctgccagaag tcactttccc ggatattcag ggcagacagc gttttatttt
    24661 ccacggcaaa gcggaccatc ttcaggtgat tccccgtcca tacggcatct gccactgaag
    24721 acagccccgc cggggcctgc agggtttcct ttgtcacagg aacctgatac cagcctgcag
    24781 gtaattttcc gccacggaca gcctggctga tggtcacaag cagggattcg taaggcgtgg
    24841 acgtttccca cgctccggct tcttctccgg ttccgcgcag gtcactgacc agctgaatgg
    24901 tacgccccgc gccttcacgg ggaacagcct gaatggaaag gttcagacca cgctctgttt
    24961 ccagaatgaa cgtaaagggc tttttgttga cggtggccac caccacaccg ccggaggcgg
    25021 tctgctcatt attggtcagg gcaccatcca gactgttcac ggcgataatc cggtcaccgg
    25081 gaacggtaaa cagattcggg ctggtattgc tgatggcaat actggcctga ccaccgttca
    25141 ccattggcac cacagtgggg gccagcgtac cgtttgcggc catcacgctg caggaaaaaa
    25201 acaggccgcc gaatattatt gccgtattat tttttctcat cgtctgtttc cccgaaatta
    25261 tccagccagg tcaccccgtt ttcccgcttc agaataagga tgtaatgttt gatatcagta
    25321 aaaggttcgg agttaccaat ccatgttttc cgaattccac ggatttccac acgaccatac
    25381 tgaggccaga cacggacact ggtctggaaa aaagcagaat tcacgttatc ttttttaatg
    25441 agtttcgctt cttcagccag aataactttc atctggttct gtgcacccgg gcggatatat
    25501 tgcagaagcg cctgatgtga agcatcgacg atttccggtg agacattcag acggagggca
    25561 ataaatgaca gcgccatctg ctgcaaataa gaggcatcgg cactgttctg tgacacggca
    25621 aagggcacat taaatcccat tggggtcacg gcagtgcgtt gctcattctg aagacggtag
    25681 ttattcaccc cctgaatgag gttaacagag aggctgagaa caataagcac tgacataaat
    25741 ataaaggcga tggccattac acgactggta cttaaacggg caccgtgttc catataacaa
    25801 tcctggtatc agttctattt aatccactgc cggaaacacg aatcgggaac attatgaaaa
    25861 ataccgcgca gcagggctgt tggcatatac cagtaaatca ggtcacgtaa ccaggaactg
    25921 ccccgccctt ttttcagttt tttaatcccg aaataaacca gaaccgctgc accaataccg
    25981 aacagatatt tcgatgttgt gataccccag ccaacacaga ttgctgcggg gatcagttca
    26041 tccagtggca ggccaaacca gcggctctgg ttggtcagtg tttccgggaa acgatatttc
    26101 ttaagtttat tctcgtctcc cgacatcgtt ttatttcctg tcagaggcca acgacgctca
    26161 tacccacagc aataaataca gagatgatgg caaagccgac caggaacttg acgtttttgg
    26221 tcatcatgta catgaccgca ccgaccagca cctcagccag aacaacccat ttaacgatac
    26281 tggagtcctt accgaaggtc gccttaaccg tatcgttacc tttagccatc aggtcgcctt
    26341 taccagcagc catagctaac tcagggaaca acatcatcag aacagcaacc gggatcactg
    26401 cacgaaccag gcgaagcata ttcagacgag tgaatttaga aaaaaacgat ttattttcaa
    26461 cgggcgcaga agcaccctga acacttaaaa cagtattcat atgattcctc tgattaatat
    26521 tgacagatga aaactaaagt tctttaaatg ttgactcgtt ctcttcgatc acctccctga
    26581 atatttcttc attataaaaa tctggataac gttgcaaatg atcctgcaac ctcaccaatg
    26641 cttcgtttgt tttcgttctt ccactacgct ctctggctcc aagtaataat ttattcgttt
    26701 cctcatcaag ggtaagtaaa acttttctac ttggcgcaga tcttaaagct cctttaatca
    26761 ccctccctcc cataaataaa agagatttag tcaaaatatt cacataagac gcacacaaaa
    26821 ataaacaaca aaatagtaaa ttacgttaac aaaaaccggc acttttagtg ccgggaatgc
    26881 ttacatttct ttacagtgaa ggctgttaat attactagcg aagtattgat atagataaaa
    26941 taacacatca gagattatag catatcgcaa ccactcatcc ctgtctgaga aaccatagtg
    27001 ttttttaaaa ttcataaata tttttttaga ttggtcaaca gagatatcca ttctctgaga
    27061 agaatactca tgactataac ccaaaagata cggtattatc ttattaaagc gatatttgtc
    27121 aggttcacaa ctaaactcct tgccctttat aaaaaagcca ctaactacgt atctttcaaa
    27181 gaatctccag acaaacaaat tcaagttatt cacttccgta ctttcaacca ccacatccca
    27241 tgatacatca ttcaatatta caccattaat aatactcact ccattcgagg acgagaaagc
    27301 atcaacttca gcagtatgaa ataaaatttg cgtatcaata tcaataccag aaaaccatat
    27361 ttcagaatca ctattctgac actttattaa gtgtaaaaaa acagagtttg agtctatgaa
    27421 ttttcctgac tctgtgcgga cacagactgg atattttgcg gataaaataa tttccggcag
    27481 gcctgattga cgtatgagtg ctctttctct agtatccatc gcacacatag gaacctcctc
    27541 aaaggattct atggacagtc gatgcaggga gggacaagct ccctgcatcg gcgattttca
    27601 agttcaaatt ttaacgtggc attaaccacg taattaatat actaattatt ctgattattg
    27661 tcatcagttt gaaccattaa aaagtctgat atttgaacaa agtcaaaatt tatttccctt
    27721 tattcatcat cattttttgg aaagaacctt tccatctcag ccgacacctt ctctctgata
    27781 tcttcaacca tattggcata ttcaaacttt ggatttcctg agacatgagg actgagagac
    27841 tcaataccca aaattttcgc tactgatgac tgtgttttta caacgcattc aagaagtagt
    27901 ttattaaact cggcctgatt aaatgcagac tctttgcgct ccatctgagc ctcatataca
    27961 cgaagaccca gttcaagaag cattgaagcc gttgctgaaa aactgacatc tttttctctc
    28021 gccccctcct gtcgacgttt ttcaataatc gcgtttattt tttcataagc atcattgctg
    28081 atatacagat tcaccttagc cattagatat tacctttttt aaacataaaa caaagttaat
    28141 ttcgaaacac tagaattcat agtgcaaaca gggacgcacc gctagcagcg cccctgacgg
    28201 tatcctataa aaaaacacac cgcgccgcta gcagcacccc taatataaaa taatgttttt
    28261 tataaaaata gtcagtacca cccctacaaa gcggtgtcgg cgcgttgctg tagctgcgtt
    28321 aacgacgctg ctttaaaaaa atcagattta aacaatataa atccacaaat acaactcaat
    28381 gatattaaag ataaatcagc aaaaacttgt ttttgcgtag tgtgtggtgc ttttggtggt
    28441 gagaaccacc aacctgttga gcctttttgt ggagtgggtt aaattattta cggataaagt
    28501 caccagaggt ggaaaaatga aaaaatggat gttagcaatc tgcctgatgt ttataaatgg
    28561 gatctgcgaa gccgccgatt gctttgatct tgcaggtcgg gattacaaaa tagacccgga
    28621 tttactgaga gcgatatcat ggaaagaatc ccgttaccgg gttaatgcca tcggtattaa
    28681 tccggtaacg ggatatggca gcggactgat gcaggtagat tcccagcatt ttaacgaact
    28741 ggcccgctat ggaattaagc cggaacatct gacaacagat ccctgtatga acatttatac
    28801 cggtgcttat tatctggcaa tagcctttaa aaaatggggc gtctcctggg aggccgttgg
    28861 tgcatacaat gccggattca ggaagaccga acgccagaac cagagacgtc ttgcctacgc
    28921 atcagaggtt taccggattt atacctggat aaagagcagt aaaggcatcc gggttccgac
    28981 cacgaagaaa tcactttccc aaattaacag tgtgcagaaa aattaatatt gtatacattg
    29041 tcaaataccg gttatgccgc gacagcggca tggaaggcca cacacaaaaa tatcatatga
    29101 gtgataatgc tgactggccg aagccccgca aagcgggatc ccatcttata tatatccggt
    29161 aactgcgcag cctcctgcca gaccattttt cgcgggcaca atatttacag actaccgaaa
    29221 agccagttga tgaaattgag gcatatcaga cgacacagca atgccgcagg atgataatat
    29281 aagtaaagcc ccgaaaaatt tttcggggct ttacttatat tatcatgact tccactccct
    29341 gaactgttcg gcaatcatcc ataatgcttt gttgattcgg atatcaccgt caatgcctgt
    29401 aatggctctt gttctggtat ttttaccgga ggcacttcgc cccgataatc caccttttat
    29461 catattctcc tgaacccgct gccaggttgt ccagagatcg ttctgtttat cctcccaacg
    29521 gcggggagta attatttgtt ctggcgtcac tggcgtttta ttttcatctt cgtacctggc
    29581 catcaatgca gctctgccaa ataaatgttg ttcgtcactg ttaagatgta tctctttcat
    29641 tgtctccata ttttcagtga ctttatcaaa gacaccgagc acttcatacg ctccctcaat
    29701 aacctgcccg acaatatcac ctttatgtgg aacgcggatt tcgccaaaat tattcccaca
    29761 caccaggcca tttgtgcaga caaaacgaaa aattcctggg atcatctgat aactggatga
    29821 accatcatgt gaattaagca ggataatttc aggaacttct ttcccgttaa tatgcccttc
    29881 cctgcgaaga cgtaacatat gtttactgta ttcgcggcgt cccaaatcac gaacccgact
    29941 ctgacaggca aagaatggct ggaaaccttc atcacgtaac ttattgatga tattgatggt
    30001 tgggatatat gtataacgct cactccggga ttcatgttta tcaccagaaa agacagatgg
    30061 aacaaactgc attaattcgt catccgttaa cgggcgctca cggcgaatag aattatatcg
    30121 accaaaacgg gaagctaatc gcataacagt aactccttta tgaaggcata aaattaataa
    30181 aagttttttc gcttaatatc atcccccctc ggttatcgtt aacaaaaaga caacaattcc
    30241 gaggggaagg accatcaaat caggtcagtt cgggactgac caacctgccg agtgaaaagc
    30301 agtaacagcg cgatcaactc atcagcgttt tttctgacaa gaggagtatg attctccagg
    30361 ctatccagta agtcatgcag ctcattattg acctggtgga gcaccttaaa gagtgcacca
    30421 gaatcccggc agctctgata tgcaagattt gcctgaacag cacgattctg catgatcccg
    30481 atttgtgcga ttgcgtcttt ctgctgatcg cctgttggct gataagtggt gttaaaagaa
    30541 aatgcgctca taattcctcc tgtatctgat gttttcagcc cccatcatct gttgagtttc
    30601 gggagccgcg gttttgacgt tttgcgggga cctgcctttt gttccctccg gtattcacca
    30661 tcacctgttt gagaaccttc gccgcaaggg gcatcactgc cacggcctga cgcacctgtc
    30721 ctgggcagaa cgcaaaattt tttaccgggt tttgggtaaa aaattttgtg ggctgttcat
    30781 ttaccctgga cagtcagtgc ggcaggttgt ggctgtctat gcccccggcg acaggggctt
    30841 aaacaggtga atggcaccgg acagaacaac gggcagggac ccgccgcaac gacgtgaagc
    30901 gcggttcgcg aaacggcgtt gctccttaca tcccggcgtc ttttgccggg atgccgggag
    30961 cgcaggccac ggcgcgacaa gggtggcgcg accgttccgg tcgctgcaaa cccgggtttt
    31021 tgcgtcaggg atggaagccc gccagggcgg agacagcttc gctggctccg ttcacgacag
    31081 cccgccccgc cagggggacg cccggaaaag gcataagcag gagaacaaca aaacagacaa
    31141 taatcagggc agcagtaatc agagtcagag cagtaatcag agtcagagca ggaggaaaaa
    31201 gagaataatc aaagggaagg tgatgtgatt tctggtaagt cataaacaga gaaggcgggg
    31261 gaaaagaacg ggcgccgcag gcgcacgttc atccccgttc ccggagggaa acggccttcc
    31321 tggcagtctg gttgttcatg cccgtacaat gccagcaagg agaaaggggc caccggcgaa
    31381 ccagcagccc ctttataaag gcgcttcagt gcagtcagac cagcatcagc cctgaaaagg
    31441 cgggcctgcg cccgcctcca ggttgctact taccggattc gtaagccatg aaagccgcca
    31501 cctccctgtg tccgtctctg taacgaatct cgcacagcga ttttcgtgtc agataagtga
    31561 atatcaacag tgtgagacac acgatcaaca cacaccagac aagggaactt cgtggtagtt
    31621 tcatggcctt cttctccttg cgcaaagcgc ggtaagaggc tatcctgatg tggactagac
    31681 atagggatgc ctcgtggtgg ttaatgaaaa ttaacttact acggggctat cttctttctg
    31741 ccacacaaca cggtaacaaa ccaccttcac gtcatgaggc agaaagcctc aagcgccggg
    31801 cacatcatag cccatatccc tgcacgctga ccacactcac ttttcctgaa aataacccgc
    31861 tcattcaggc tgtttacggg taatctgtgt gattgttgcc gcatcacgcc gcctcccgga
    31921 gtttgttcgg caccatccac cgctccatct cacggacagc gtgaccggcc tctcttgctg
    31981 caagactggc ttccgcaata acctgccaga acggcatatc ctgccactgc cagcgttcca
    32041 ttccggccag tgacggaaaa cgctcacccc actgccagaa cagggtttcc aggtcatgct
    32101 ctgccacccg gtcactccag cgtgcatacc agcttccgac tgtttccggc gaatggaatg
    32161 ccagttcgat ttcagcctga gcgaccagcg cctggtacgc acgggctttc tgctctgcct
    32221 ccctcgcctg ctggcggctg tacttctgca tgaccagcgc ggatttatgg cgactgcgtt
    32281 ccctgacacg aaaccgcacc gacgggaaca agcgttctgc tgcatcgcct ggaagaggaa
    32341 ggaggcagac ctcgccctgt gtttcaatca gcacatcgat ggcatacagc cactgaagac
    32401 ggctgccgtg cagttcttct gcggtcaggg acggcatgaa aaatcgcagg tcagacagcg
    32461 atatcctgcg gcttccgttc agacagcgga aaaacgcccc cgcatacgga tattccgcca
    32521 gcgtgtttcc ctggtggcgg cgtttttctg tctccgccac cgcccgccat gcggcctgtt
    32581 gctctgcgct gaggggaacc agtgcctgtg aacgtgcgct catgcctgac ctccttccgc
    32641 catcaggata tggatgatgt cactgacgct gtacccctgc gcgtccagtg acgccgccag
    32701 tgccagtgaa tggctgacgg cttccggtgc aaaccgccgc tgtacctgaa ccaccgcaaa
    32761 cggctcacca ccggcattca ccagaaccag cacccagacc tgagagacat cgcccggtga
    32821 acataacgcc aggtgaaaac gttcgtgggc aggcgtgaaa cggacctgga cggggaaaaa
    32881 tccgccaaac tcgctgccgt actcgccgtt catcgtccag ttgtccggcg catccagttc
    32941 ccgcatcacg gcgtgtgtca gctcacgacg ttcatcactt ccggcagccc ggatatcttc
    33001 atattcctgt gcgctcatgg tctgtaatac attcagggtc agttcagttt tcatcacggt
    33061 atctcccttg ctgccgcccc gtctccggcg gggcggtgtg gttgttcatc aggcggcatc
    33121 agccaggtta tcggtgcgtt cagtggtatc tgtctgtgga tgtggtgcac acatccagcc
    33181 aggcacccag cggttgtctt tcatatggtg ctctgcatgt tcagccgcat cacctttctt
    33241 catcttctcc gcgtcccgtg cggcaccgga cagccctgcc tcattcaggg ctgcgataat
    33301 ctgcggcttt ttcaggtgtc cgaagaagtt tgcttttgtc ggctgccacc agtcgcgcat
    33361 gtgaaagccg atggcgcttt ccagcgtgtc aagcggactg cgtgacgtgt gaccacactc
    33421 acgggtctgg acaccgttaa ggctgcatgc ggtgcagaaa ctgagcaggg ataacagcac
    33481 ctcctgactg agtgacagga atgtcgtcat gtcccgtgac catccctccg gtaacagggc
    33541 agcaagacgg gctttttctg ccatcagcgc catgaatgcg gcaccttcct tccccgatgg
    33601 cgcatcgctg gtcagcgaat aatgtttaca ttccaggctg atttgtgctg gtttactgta
    33661 cgctccgctg ccaaacacat tcaggcagag cgtccatgcc agcagtgtca gggatttgtc
    33721 cggttgctgc atcagtgctg cctggactgc cagcgtgcgt tcggaagaca ttttcgtcag
    33781 cagtggcaga ctgatttcct ccactgacgc tttctcctgc acctgttccg tacggtcagc
    33841 gtcgtcagcc acgtcatcct cactgcgcaa ctgcacacca cgctggacac acacgttgcc
    33901 ataacgccag gacaccacca ctccacctcc ggcacgcatc tccggcgtcc acgccctgac
    33961 cttcgccatg cagcgcatca gcttcatttc tgcttccagc aggtcagatt cctcacactg
    34021 gttttccagc gcgtcgtaac gcgtcatcag ttcgttcaga cgttcttctt ccgcctccgt
    34081 cagcaccgct tccggctccg gcagacagcg gtatgttccg gcatcctcac ggcactcacc
    34141 gacaggctcc atgcgtccgg cgcaccattc ccagccttcg gcttcccgaa ggaactctgc
    34201 gacagcctgg agtttttcca gcagggcggc atcgagcgcc acgcagtcca catagccacc
    34261 ttcgtcgtcg ctgaacaaat cggtgcgcag ttcgtctggc gagaaggcat cagcccccac
    34321 gaagcggaat ttactgttcc ccgccaccgc cacttcactt tcggtcacca gacgacgaat
    34381 ggtctgtact tccggtttac cgccccatcc cgactgacag gcggcttcaa acacctgcac
    34441 ctgacgcgcg gtgtcgttct ccagcgccag cgcctgacag tgttcggtgg tgatgcggtc
    34501 ttctgccagc gcatcgagga tgacaggcgc aaggtcagcc agtttcagca ttcgctgaac
    34561 gtggcggggt gaatagccca gcaaatcacc gatttgtgca ggtgttttgc cttcctgcgc
    34621 cattgcgcgg aatccggcaa tctgttcggc agggtgcata tcccgacgct gaccgttttc
    34681 agtcattgat gcagccgtcg ccagctcctg cggaataacc ttcacgcgta caggccagtc
    34741 agccggaagg atgccacgct ctgccagcat gttgagtgct gccagtcggc gaccacctgc
    34801 ggcgacaccg tggcggtcac caggcagggc atgaacaacc agattctgca acaggccaac
    34861 gcccttaatg gactccgcca gttcgctgac ggactctgcg gaatacggca ccgtgcgcac
    34921 attcagcggt gatttaatca gcgaggccag cgacacgctc acttcctccg tctgctccag
    34981 cagggcagac aggactgttt cctgcgtttt tgcaggttta cggctggatt tacgctccgt
    35041 ttttgtctta gactctgtaa ctgacatata tttctccttt gaccgggatg gttgtatgtc
    35101 tgccggacgc gttcccgtcc ggcacctctc ttacttaaga atgccgaaat tccggatgac
    35161 atggttcatc cagtacccgg caccgtcctc aaaattccag gtacgccaga ccaggccact
    35221 gttattgcgg acaaccaggc gaaaatgcgt tccctgatca tcctcgataa agacattccg
    35281 gtactgtgcg gcaacggctt cagcctgttc gcgggtaaag gagccgtcag gcagcccctg
    35341 aaatattctg aaatattcgc tcatcctcat atctccggtg atgccccgca ctggacgggg
    35401 cggttgtcac tgtctttcgt tagaacggaa tgtcgtcatc gaagtcatac gcgggttcag
    35461 acggctgctg aggctccggc tgcgcggcct tacgtccacg gccttttgtt ttcgcgctcc
    35521 cttttttcgt cgtgtcagca ctctgtggct gaccattctg ctgaggctgc ggttgcgcct
    35581 gagcgttctg ctgtggtgca ctccccagca tctgcatggt gcccgtggtt ttcacaagga
    35641 tttcagtgac gtaacgggta atacctttat cttcccagct acgggttcgg agttgcccct
    35701 cgatgtagac ctgcgcgccc ttgcgcagat attcacctgc cacttccgcg agcttgccga
    35761 acagcaccac gcgatgccat tctgtctgct cccgcatctc ccccgtctgt ttgtcacgcc
    35821 agctttctga cgtggccacc tgcaggtttg ccactgcgcc cccgttgggg atgtaacgga
    35881 cttccggatc ttttcccaga cgaccgacaa gaatgacctt gttaatgcca cgaactgcca
    35941 tgatgtgtct ccttctgttg atgttttcag cccccatcat ctgttgagtt tcgggagccg
    36001 cggttttgac gttttgcggg gacctgctct ttgttccctc cggtattcac catcacctgt
    36061 ttgagaacct tcgccgcatg gggcatcact gccacggcct gacgcacctg tcctgggcag
    36121 aacgcaaaat tttttaccgg gttttgggta aaaaattttg tgggctgttc atttaccctg
    36181 gacaggtgcg gcaggttgtg gctgtctatg cccccggcga caggggctta aacaggtgaa
    36241 tggcaccgga cagaacaacg ggcagggacc cgccgcaacg acgtgaagcg cggtttgcga
    36301 aacggcgttg ctccttacat cccggcgtct tttgccggga tgccgggagc gcaggccacg
    36361 gcgcgacaag ggtggcgcga ccgttccggt cgctgcaaac ccgggttttt gcgtcaggga
    36421 tggaagcccg ccagggcgga gacagctccg ctggctccgt tcacgacagc ccgccccgcc
    36481 agggggacgc ccggaaaagg cataagcagg agaacaacaa aacagacagt aatcagagca
    36541 gcagaaaaaa gaagaacagt agcagtagca gtagcagtag caggaacaac aacatcatca
    36601 gtaacaggca aatgacaggt ctgaaaagac agaagcccac agcagaacgt ccgcgtaatg
    36661 aaaggggcgg cgcgtaagcg cggccccttt cagcgccacc ggcgcgcggt cttttcccgg
    36721 cgcgggcaga cgggcattta tcctcccgcc tgcccggatt catcaggcac gcagacgaat
    36781 aatcatcgtc ggaacacggg tataagcaaa tgtgccgcac ggcagcacct cgcggacatc
    36841 agaaaacggt aacagcttct cctgctggcg cggcccgttc agacagacgg caaccagaac
    36901 gccccccgga cgcaacaggg aaaaagcccg caggatatgc cggatatcct gcccgtgact
    36961 gaacggcgga ttcataatga tccggctgta atactgcacc ggctgccatt ccatgaagtc
    37021 gccacactgt accctgacac cgttaaagtt ttcccgcaga taccggacca gcccgctgtt
    37081 aatctccacc gcatcacaca tggcctccgg cgcggtatcc cgaatcgccc gcagtatggc
    37141 cccggtgccg gcagagggtt ccagaatgtg atcacggttg ctgatttccg ccagcgtcac
    37201 caggcgatca cagaccgggg caggagtcac aaaaagctga gattcagccc ggatttcagc
    37261 aaccggcgcg gcgttatcca gccttgcgcg catcagccgg atacgttctc ttaactgact
    37321 gtgcatgtca ttatctccgt catcccccgg ggatttcccg gggggatata ccttatttct
    37381 gcgggatttc cgtgattttc atgtcggtga tatacacatt caccagacgg aaattattgt
    37441 ccattgtgcg gcggtagcgg tatgccccgt ggtcttctgc ttcttccaca ctgcgcacgg
    37501 ccttacagtc ccggggcagg gcggcccact gcgcctttgt catttcccgg aaaccttccc
    37561 ccggatagtt gactaccggc ggacgcttcg cggcctgact ggcgacggca gcctcttctg
    37621 ccgatggtgc tttgtagtcc gtgatgcgat cgggcgtcac tttcatcgtg ccgctgtacc
    37681 cgagaaaact gtaatttggc gttgtgactg aactcaccgc cccgttgctt ttgttcacgc
    37741 ggatgatagt cagccactcg ccccggctga aaacctgtcc gcccggctca aaatcctgtg
    37801 tccgggtaac cacgccgccg ctttcgtcca gcatggcacg ctcatagatc aggcggttct
    37861 gatagtggtt aacccagcgt tgctgatgct gaatctgccg ttcatgacag cggatcgcaa
    37921 tttcgcgggc ctgctccgtg gtgatgatgt catcatccag ggccgaccat aacgaccggc
    37981 tcccttcata ctggcttttt tctgccgggc gcggatacgt atccagcggg aaacaggcgc
    38041 tgatatggtc atgactgctg atgagttttg ccatattcag atccagcgat tcagcccgcc
    38101 acatcgtcag atatttctgc gactgtgcga tggtcttttc agccttgcgc aaatccgctt
    38161 cgattttttt gatacgacgc cagcgaacgt ccgggcgttc tttatacttc gcgtgaagca
    38221 gtgccgaccg cgcccgctcc tcccagtatt ccgcacgttc aaagagcatc acggcacgtt
    38281 tcatgccgtt ttcaatacgc tgcgcatcac gacgggcgcg gcgttcgctg tgatgcccca
    38341 caagaatggg ctgaccgggc gggatcatcg cggccagtct ttccacttca tcaagtgcct
    38401 gtgcggattc actggcccgc tttccgctgt atccggtaaa ccgctccgcc cgtgcttcct
    38461 gacgttcagc gagcgtgctg tcttcatcct caatctctcc ggcaagtgag aggagcacgt
    38521 cttcccggcc cggtgtccag gctggcgcga caaacagcgc ctgttttggt gcccagcgaa
    38581 aaccagcatc atgcactttt ttatacgtct ccgggtcaag acgtgacacg gcatacaggc
    38641 gcagtttgtt gtcatccggt gagtaggtcg cgcggtacat ctgcacaggg ctggcggctt
    38701 ccggggcggt catggtgatt gctgctaatg tcataaaatg ttctccttcc gtctgttgcc
    38761 gcccctcgcg gggcggctca cctcatcagt taaaagcgcg tttcagttcg agtgtgcgga
    38821 gcacctgccc gaaagccgtg tttttcatgg cggggatgtg tacaccacgc ccccggtgaa
    38881 aactccgggc tatgtgtcgc ccgaaggctt tcaacacggg gtcaatttcc ggcgtgggga
    38941 tgtcttcgcg tctgaaacgg ggatgacggc ggggctttac agccgctgcg gaaggtgaaa
    39001 caatgcttac agtcataaat ggattcctta tgacaaattg gttaacaatg ccgtcaaaat
    39061 tggtcgcaca attcttacgg cgtggattaa gcaggtgcct gacctgcttt tttcatatct
    39121 gcatctgacg ctttccggtg ttttcctcct tctgttcctg cgttctcatt acctgtcagc
    39181 cggatgactg acagaacaaa taaatgcgta ttgccggagc ctgaaataaa tcctgatttt
    39241 taaagaacat cacccgttga gtttcgggag ccgcggtttt gacgtttcgc gggaacctgc
    39301 tgctgtattc actgtggtat gaacttcacc cgtttgagaa ccttcgccgc aaggggcatc
    39361 actgccacgg cctgacgcac ctgtcctggg cagaacgcaa aattttttac cgggttttgg
    39421 gtaaaaaatt ttgtgggctg ttcatttacc ctggacagtc agtgcggcag gttgtggctg
    39481 tatcagcccc cggcgacagg gactcatacg ggtgtgtggc acccggcagg caatgagcag
    39541 gggcccgccg caacgacgga gagcgcgtgc gcgaaacggc gttgcccctt acatcccggc
    39601 ggccttttgc cgggatgccg ggagcgcagg ccacggcgcg acagaggcgg cgcgaccgga
    39661 acggtcgctg caagccaggg gttttcacgc atcggaccgt agcggcagcc cgaaaccgcg
    39721 ccagcgggtt cggcggagac tggcaggtgc gccagcagct gcctgtcgga gtcgggcctg
    39781 acgaccggat gccgccaggc agccgggagc cgaaggtgcg cccggcagac agaacaacac
    39841 tgcagtagtc agaatcagag cagtagataa aggaacaaca acatcaggaa cagttccata
    39901 acataagaaa gaaacactcc tgagtcagac acatcacaga cgggaagggt aacgggcgac
    39961 gcgccagcgt ggcccgcttc ccttccggca ggcgtcagcc tgccaggggt tttcgcgcgg
    40021 gctgacaggc ggcctccacc gcctgcccct gtatcacctg tttatgctgc cctgaaggat
    40081 gtagtgaccg ccgaggaata cagcctgctg attcgccaca aaatcgcaga tttcatcaac
    40141 aatctgcgca gtggtgagcg gtttctgccc catttcacga cgccgcgcat taatcacggc
    40201 tttgtaattg cgcagaattt cgcgggtttc tgtgctgata ttcatgacat gtgctccccg
    40261 tccagcgcct gtaacacaac gtccattgct tcgcgttctt ccctgacgaa tgcgtcaccg
    40321 tcgcccccgt tctcgctccc gacggcatac aggcgcttca tttcactgcc tgccagcgcc
    40381 atcacttttt ccagcatcac cgccggaact gtcacctgac ggctggcgcg cttttcttcc
    40441 atcagctcac gaacaacgtc gtgaacaatg ctgacatccg caccatgcgc aaaggcatcg
    40501 tccagtcgct gcatgacggt ttccagctca tcgtctgtca gactccagtc ctgggctatg
    40561 tgacgcacat ccccacgggt cagcaccacg gcaaaaacgc attcactgtc cggcacgtct
    40621 gtgttcagca cttcgcggat aatttcttta acagtacagt acatgttctc gtctccttta
    40681 caggggcggc attgccgccc cgtccgttaa tcagtccagt gccgcgtaaa tcgcgttgca
    40741 ttcggggtga aattcgatgt gacgccacaa ctgcgcatcg cgcatccggt aaagctgggt
    40801 cagtcctgca tccccgctgt cgtggtacag ccacaactgg cggttaatca caagggaagt
    40861 cagaatgatg cctgcggcat cagcactcac ggtacggtca aaccagtttg caccgttcac
    40921 catgtggaaa cggtcgccat ccggcatcat gtaaccgcca ccgtccggca ggcggatgaa
    40981 gtgccagaag cctccctgat attccggcat cattttttgc gccagcgcgt acacatacgc
    41041 ctcggcatac agaaaatcgt tgccaaacag atacggtaga aaggagagac ggtcgcactc
    41101 ttcaacgttc agagtgacag gtttcgcata ttgcatgatg ttttcctccg ttgttcacat
    41161 attccgcgac accgggcggt gccgtgcctc cataaaagcc gggcgataag gcggggatgc
    41221 acagggcgca agggaagccg cagcctgacg cacacggctg gcgggagttg gagggtgaga
    41281 ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc agacgggaag gagggaccgg
    41341 gtgaccgcag cggcgaagcg tacccttgca tccctgccag cccggctaag atggaggcac
    41401 ggcacagccc gcacacgacg ctgaggccac ataactgaaa aaccacaagg ccatgccctg
    41461 catggccggg taaagcggag ccggcggcga cttcctttgt gctctgtccg gggaccggca
    41521 ggaaaaaggc ggggatttac cccgccaacg tcatcatttc cggatttttg ccagggcatc
    41581 cgccatgctg cggatttcgg cccgggcttc ccggaccacc tcccgcagaa caccacggtc
    41641 aaaccagcgg cggggcggtt catccggtat acgcagcaca tcgcaggcca gcccactgga
    41701 caggatatgc tcaatacaat cgctgaccag gtacatttcc accgacgaaa aacgtgctcc
    41761 ccggttcacg atagcatcga gcaccacggg caggttttcg tcatcttcat cgagccagca
    41821 ggggtgatcg ctttccttca caaccagacg aaagcggcgc gtctggcgag tcagcaacgt
    41881 ctgaccggtg gtggtcagcg ccgcccggat acgccgcgca tcttctgcca cccggcaact
    41941 gacactgcgt accgcatcgc gcaccgccgc aagtacagcc ctgatttcgt cttcggtcag
    42001 ccattctccg gcatacggga aagacacctg cgttccgtct gccggaagta ccggcagcga
    42061 ctgccactga ctgccggaca gtaccagttc cgccccggtt ccggtcagtt tctgaatggc
    42121 ggaggccagg agcggcggca gtacgccgcc gtgacggttg ataagtaacg tcgccagccg
    42181 gggcgcatcc agcagaacat aaatgctgat ccagcccgga tacttcgggt tacgctggtc
    42241 aacatccgtc tcttccggcc agccctgcgc cagcagcagg ttgcgggcgt gacgacaaat
    42301 cagtgcgttc agcgtgctgt tttcgttcat gattaagcgt tctccctgta tcgccgcccg
    42361 cagggcaggc ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag cagggtgcgg
    42421 gcctgattaa ccacctcctg acggcgggtg acgcattccg ggacggcgag gccatgatcc
    42481 cgggcggccc attcacgttt ccacaatgcc tgttcagcgg tctgaatcag ggacgccagc
    42541 agttcagcag ggacggtcac caggcggttt tcgctctcgt tgcggataat ctccatcacc
    42601 gcagcggaag aaataccgga ctcaatccgc tggtcttccg ggatgtcctc caggcgtgcc
    42661 aggacggtcc tgatttcatg accggtcaga gaaatttcca taccgtcagc cagtgcctga
    42721 atctcttccg gggaccagat aaccagcatc agcggcatgt ctcccggata ctttactgcc
    42781 agctcccggc atagcgtttc gcatgttccg tacattctcg tctccttctc aatctttaaa
    42841 gcacgcttcc agttccagaa tttcggtata tgcctccatc aggaggcggt ttgcgcgaaa
    42901 cagcgtctga cgcaccagac gttccggcag ggtgtcggca ttaaagctgt accgctgctc
    42961 catctcattc atggttgtac gcaggtcgca catggcattg gccacctccg ccacttcggc
    43021 gcgcagtttt tcgtgtcctg catagttcat ttacgcagcc tccggcataa accagtcatc
    43081 attcgcggcc ccctgctgca tggcccgttg cacggcggca aggcgttgct gcccggcacg
    43141 gtgatactgc tcaagcagct cgataccgat ataccggcgt ccggactgga gggcggcgac
    43201 gcaggttgag ccgctgcctg caaacgggtc cagcacaatt gcgttcgggt gtgtgaagct
    43261 ctcaatcagc ggttgcaggc tggtaacggg cttttccgtc gggtgatggc gattgcccga
    43321 atatttccag cccagcacgt ccggcagcgg gttttgtggc agacgtggac ggccttttgc
    43381 caggatgtag gcacattcgt ggcgatagcc cacatatgcg gccttcgatg tgtagttttt
    43441 ggtgaagacc aggtgaccaa caacgctgaa tcccgcattt ttccaggcgg ccataaagcg
    43501 atcgacgcgg ttccagccat agaagctcac cattaacgcg tcttttttga gtacgcggta
    43561 catttcatta caggctggtt gcagccattc atcggtttta tcgccggcga tggtgcgccc
    43621 ggaacggtca cggaaaccga cgagatacgg cgggtcggtg aggatgaaat cgacagcatt
    43681 gcccggaaac gtggccataa cacgcacgca gtcacccagg ataaaacggg acataacagc
    43741 actccttctc ttacagaggg caccgggagg gcacccctgt ggtgcccgtt ccccgctgcc
    43801 tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac cgcagccgtc
    43861 gtctttttga cggcgaggat atgccgcgaa agcggcttga agggcgttca tcagaaaagg
    43921 gcgacgggct ggcgtacact gtcaccaacg ccagccggga gcggtaaaag cgcccgtccc
    43981 gtgcccggcc ggacgcggga cactgaccac aggcagcgtt cctgccgcgc cgcgtcagcc
    44041 ggcgcagtaa aaagaggagc ccggcgacat cttttgttac ccgccaaaca aaacccaaaa
    44101 acaacccata cccaacccaa taaaacacca aaacaagaca aataatcatt gattgatggt
    44161 tgaaatgggg taaacttgac aaacaaaccc acttaaaacc caaaacatac ccaaacacac
    44221 accaaaaaaa acaccataag gagttttata aatgttggta ttcattgatg acggttcaac
    44281 aaacatcaaa ctacagtggc aggaaagcga cggaacaatt aaacagcaca ttagcccgaa
    44341 cagcttcaaa cgcgagtggg cagtctcttt tggtgataaa aaggtcttta actacacact
    44401 gaacggcgaa cagtattcat ttgatccaat cagcccggat gctgtagtca caaccaatat
    44461 cgcatggcaa tacagcgacg ttaatgtcgt tgcagtgcat cacgccttac tgaccagtgg
    44521 tctgccggta agcgaagtgg atattgtttg cacacttcct ctgacagagt attacgacag
    44581 aaataaccaa cccaatacgg aaaatattga gcgtaagaaa gcaaacttcc ggaaaaaaat
    44641 tacattaaat ggcggggata cattcacaat aaaagatgta aaagtcatgc ctgaatctat
    44701 accggcaggt tatgaagttc tacaagaact ggatgagtta gattctttat taattataga
    44761 tctcgggggc accacattag atatttctca ggtaatgggg aaattatcgg ggatcagtaa
    44821 aatatacgga gactcatctc ttggtgtctc tctggttaca tctgcagtaa aagatgccct
    44881 ttctcttgcg agaacaaaag gaagtagcta tcttgctgac gatataatca ttcacagaaa
    44941 agataataac tatctgaagc aacgaattaa tgatgagaac aaaatatcaa tagtcaccga
    45001 agcaatgaat gaagcacttc gtaaacttga gcaacgtgta ttaaatacgc tcaatgaatt
    45061 ttctggttat actcatgtta tggttatagg cggtggcgca gaattaatat gcgatgcagt
    45121 aaaaaaacac acacagattc gtgatgaacg ttttttcaaa accaataact ctcaatatga
    45181 tttagttaac ggtatgtatc tcataggtaa ttaatgatgg acaagcgcag aaccattgcc
    45241 ttcaaactaa atccagatgt aaatcaaaca gataaaattg tttgtgatac actggacagt
    45301 atcccgcaag gggaacgaag ccgccttaac cgggccgcac tgacggcagg tctggcctta
    45361 tacagacaag atccccggac ccctttcctt ttatgtgagc tgctgacgaa agaaaccaca
    45421 ttttcagata tcgtgaatat attgagatcg ctatttccaa aagagatggc cgattttaat
    45481 tcttcaatag tcactcaatc ctcttcacaa caagagcaaa aaagtgatga agagaccaaa
    45541 aaaaatgcga tgaagctaat aaattaattc aattattatt gagttccctt tatccactat
    45601 caggctggat aaagggaact caatcaagtt attttcttac cagtcattac ataatcgtta
    45661 ttatgaaata atcgtttgca ctgtctctgt tattcaggca atttcaataa aggcacttgc
    45721 tcacgctctg tcattttctg aaactcttca tgctgcattt cgatttttct tctaaaatca
    45781 taacttgcat acctgttctt tttatgtata tccagatcta tttttgtgac gagttcaaac
    45841 cacaatgata gcactcccca tgcagcgtta tgaaacattt ccgcaacaga tttagagtct
    45901 gtattaaccg cttcagtcat gtagtcgcta atacgttctg cagtgatttt cgttatttca
    45961 tcgtaattca ttttatcccc tacaagacta tttaatggaa tgaaaaatat ttggtctgat
    46021 aacaatgctc ctgaattaac acctgcaggc tatgttaaag ttgccctcac acacaatcta
    46081 cctgttttct cgtgtgtgtc acaggcgcaa tacataaata gtctggccat taagttcgta
    46141 acggatttcg tactcgccag caaaaatccg tctgacatcc ctggtttcaa actgaaacaa
    46201 ctgttcatcc catacgtgga tgagttaaca gaatgaccgg cgcctgtgtc agggactaca
    46261 ccgttttggc tgcagctggt ttactgacca acgccagaaa atcatataac tgcgtcaatg
    46321 tcagcgtatc cagcgcgact gtattatttt tctctgcaac cagaatattg cattaggtta
    46381 ttaccaggtg ttctaatgta caggtaaaga cgttctttat aatctgaaat ctctactttt
    46441 aatccctcag actttgcctg ctgaaagccc tcgtcaaggt gcgcttttat taggttaatg
    46501 tctggatgtc ttgtatcggg ggcaattctg tattggtgta acgatggggt ggatgttccc
    46561 tgcaataaga cctccagata gagattggca caaccattcc cattatttct aatagtttat
    46621 tggtaatcac aaactgtata tttagccatt atattgaatc ctgtgaagta aacggcgacg
    46681 gctcaaaatt ttatcaaacc taagataatt ctaccatcaa agttaataat ttgaatttat
    46741 atgacggctc tatggggggg taaaaatccc cgtgcagaga ggcgattttc agagttgaaa
    46801 acgtcgtcgt taagaacacc aaagtcagta ccaccaaccc ggaaagtagc ttcatgcacc
    46861 gggacaacag cagttttggt gtccgaaatc gtacaattct gtacatctac gcaaggaaat
    46921 gtacggaagt aagccaaatg gttatcggac ggtcggaaca tttcgtacag cctttacgtt
    46981 cagtgagtta gctggcctag atgtaaccac tcgttcaact caattctctt agcgtgggaa
    47041 aatttccgtt ttccaagcgg ccccctcaag cggacccctt atagtacaat aattttcgat
    47101 atccaaactg cccccaatta catctccagt ctctggcagc agagatcata tatttccatt
    47161 gccgccgtag ccacgatatt ctggctattc acaaaaacaa tttagcccca aagcgcagta
    47221 aaaaagtaca ctatgaaggt gtatgatacc ttcatagtgc ttaagtaatt atgagtaata
    47281 taatctgtgt aaactactaa ttgcttcacc gtcagtgatt aaatgcgtag ctgttccttg
    47341 tcttatataa tacctatttt caaaatgaca gacttctccg acacttttta tttcgatttt
    47401 taaaacatgc agtccttcat atgaacataa tgaaatatct tttagtattt gttgtttcaa
    47461 agcctcatta aaattaaatg attctatttt ttgttttatg aacagaaagt aattatctat
    47521 gttttttgat tgaattacag cttcatgatc aattccattg atataatagc cattgcactc
    47581 aataggggta acaccatata aagtcttaac tctgtctgcc gtagctttgg tatcggaaac
    47641 accaactaaa atataaccat tagattctct accgatgtta tttatggcga cagccgtttg
    47701 gactatatta atgaaacatt cttcgtctat tttattctcg cctgagagaa taaataatcc
    47761 ctgtt
//
LOCUS       DADWTH010000022        47520 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.022, whole genome shotgun sequence.
ACCESSION   DADWTH010000022 DADWTH010000000
VERSION     DADWTH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.022"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(15..107)
                     /locus_tag="JNY02_001294"
     tRNA            complement(15..107)
                     /locus_tag="JNY02_001294"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Ser,seq:gct)
     gene            complement(423..608)
                     /gene="csrA"
                     /locus_tag="JNY02_001295"
     CDS             complement(423..608)
                     /gene="csrA"
                     /locus_tag="JNY02_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBB9432903.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(843..3473)
                     /gene="alaS"
                     /locus_tag="JNY02_001296"
     CDS             complement(843..3473)
                     /gene="alaS"
                     /locus_tag="JNY02_001296"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBB9432904.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3601..4101)
                     /gene="recX"
                     /locus_tag="JNY02_001297"
     CDS             complement(3601..4101)
                     /gene="recX"
                     /locus_tag="JNY02_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBB9432905.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4344..5405)
                     /gene="recA"
                     /locus_tag="JNY02_001298"
     CDS             complement(4344..5405)
                     /gene="recA"
                     /locus_tag="JNY02_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBB9432906.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5485..5982)
                     /gene="pncC"
                     /locus_tag="JNY02_001299"
     CDS             complement(5485..5982)
                     /gene="pncC"
                     /locus_tag="JNY02_001299"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBB9432907.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6127..7212)
                     /gene="mltB"
                     /locus_tag="JNY02_001300"
     CDS             complement(6127..7212)
                     /gene="mltB"
                     /locus_tag="JNY02_001300"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBB9432908.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7468..8031
                     /gene="srlA"
                     /locus_tag="JNY02_001301"
                     /gene_synonym="gutA"
     CDS             7468..8031
                     /gene="srlA"
                     /locus_tag="JNY02_001301"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBB9432909.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8028..8987
                     /gene="srlE"
                     /locus_tag="JNY02_001302"
                     /gene_synonym="gutE"
     CDS             8028..8987
                     /gene="srlE"
                     /locus_tag="JNY02_001302"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBB9432910.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKLGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8998..9369
                     /gene="srlB"
                     /locus_tag="JNY02_001303"
                     /gene_synonym="gutB"
     CDS             8998..9369
                     /gene="srlB"
                     /locus_tag="JNY02_001303"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBB9432911.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9373..10152
                     /gene="srlD"
                     /locus_tag="JNY02_001304"
     CDS             9373..10152
                     /gene="srlD"
                     /locus_tag="JNY02_001304"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBB9432912.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10257..10616
                     /gene="gutM"
                     /locus_tag="JNY02_001305"
     CDS             10257..10616
                     /gene="gutM"
                     /locus_tag="JNY02_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBB9432913.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDILFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10683..11456
                     /gene="srlR"
                     /locus_tag="JNY02_001306"
                     /gene_synonym="gutR"
     CDS             10683..11456
                     /gene="srlR"
                     /locus_tag="JNY02_001306"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBB9432914.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11449..12414
                     /gene="gutQ"
                     /locus_tag="JNY02_001307"
     CDS             11449..12414
                     /gene="gutQ"
                     /locus_tag="JNY02_001307"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBB9432915.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12411..13925)
                     /gene="norR"
                     /locus_tag="JNY02_001308"
     CDS             complement(12411..13925)
                     /gene="norR"
                     /locus_tag="JNY02_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBB9432916.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRVVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14112..15551
                     /gene="norV"
                     /locus_tag="JNY02_001309"
     CDS             14112..15551
                     /gene="norV"
                     /locus_tag="JNY02_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBB9432917.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15548..16681
                     /gene="norW"
                     /locus_tag="JNY02_001310"
     CDS             15548..16681
                     /gene="norW"
                     /locus_tag="JNY02_001310"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBB9432918.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16809..19061)
                     /gene="hypF"
                     /locus_tag="JNY02_001311"
     CDS             complement(16809..19061)
                     /gene="hypF"
                     /locus_tag="JNY02_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBB9432919.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19214..19741)
                     /gene="hydN"
                     /locus_tag="JNY02_001312"
     CDS             complement(19214..19741)
                     /gene="hydN"
                     /locus_tag="JNY02_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBB9432920.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19890..20900)
                     /gene="ascG"
                     /locus_tag="JNY02_001313"
     CDS             complement(19890..20900)
                     /gene="ascG"
                     /locus_tag="JNY02_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBB9432921.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21160..22617
                     /gene="ascF"
                     /locus_tag="JNY02_001314"
     CDS             21160..22617
                     /gene="ascF"
                     /locus_tag="JNY02_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBB9432922.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            22626..24050
                     /gene="ascB"
                     /locus_tag="JNY02_001315"
     CDS             22626..24050
                     /gene="ascB"
                     /locus_tag="JNY02_001315"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBB9432923.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFHEKGVTIDKALGDDEILKNTVDFVSFSYYTSRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24165..24584)
                     /locus_tag="JNY02_001316"
     CDS             complement(24165..24584)
                     /locus_tag="JNY02_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9432924.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24581..24892)
                     /locus_tag="JNY02_001317"
     CDS             complement(24581..24892)
                     /locus_tag="JNY02_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HBB9432925.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25065..25808)
                     /locus_tag="JNY02_001318"
     CDS             complement(25065..25808)
                     /locus_tag="JNY02_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432926.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(25860..26330)
                     /gene="hycI"
                     /locus_tag="JNY02_001319"
     CDS             complement(25860..26330)
                     /gene="hycI"
                     /locus_tag="JNY02_001319"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBB9432927.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26323..26733)
                     /gene="hycH"
                     /locus_tag="JNY02_001320"
     CDS             complement(26323..26733)
                     /gene="hycH"
                     /locus_tag="JNY02_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBB9432928.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26730..27497)
                     /gene="hycG"
                     /locus_tag="JNY02_001321"
     CDS             complement(26730..27497)
                     /gene="hycG"
                     /locus_tag="JNY02_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBB9432929.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPSCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27497..28039)
                     /gene="hycF"
                     /locus_tag="JNY02_001322"
     CDS             complement(27497..28039)
                     /gene="hycF"
                     /locus_tag="JNY02_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBB9432930.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28049..29734)
                     /gene="hycE"
                     /locus_tag="JNY02_001323"
     CDS             complement(28049..29734)
                     /gene="hycE"
                     /locus_tag="JNY02_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBB9432931.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIACDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29752..30648)
                     /gene="hycD"
                     /locus_tag="JNY02_001324"
     CDS             complement(29752..30648)
                     /gene="hycD"
                     /locus_tag="JNY02_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBB9432932.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            complement(30651..32558)
                     /gene="hycC"
                     /locus_tag="JNY02_001325"
     CDS             complement(30651..32558)
                     /gene="hycC"
                     /locus_tag="JNY02_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBB9432933.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGAMSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGIILLGLGLGLGLGLGLGLGLGLGLGLGAGV
                     TGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKM
                     PVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAIT
                     GALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLP
                     MLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGY
                     DHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELA
                     VLVVIIVSRGA"
     gene            complement(32555..33166)
                     /gene="hycB"
                     /locus_tag="JNY02_001326"
     CDS             complement(32555..33166)
                     /gene="hycB"
                     /locus_tag="JNY02_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBB9432934.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPLLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33291..33752)
                     /gene="hycA"
                     /locus_tag="JNY02_001327"
     CDS             complement(33291..33752)
                     /gene="hycA"
                     /locus_tag="JNY02_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBB9432935.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33964..34314
                     /gene="hypA"
                     /locus_tag="JNY02_001328"
     CDS             33964..34314
                     /gene="hypA"
                     /locus_tag="JNY02_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBB9432936.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34318..35190
                     /gene="hypB"
                     /locus_tag="JNY02_001329"
     CDS             34318..35190
                     /gene="hypB"
                     /locus_tag="JNY02_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBB9432937.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35181..35453
                     /gene="hypC"
                     /locus_tag="JNY02_001330"
     CDS             35181..35453
                     /gene="hypC"
                     /locus_tag="JNY02_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBB9432938.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVSFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35453..36574
                     /gene="hypD"
                     /locus_tag="JNY02_001331"
     CDS             35453..36574
                     /gene="hypD"
                     /locus_tag="JNY02_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBB9432939.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            36571..37581
                     /gene="hypE"
                     /locus_tag="JNY02_001332"
     CDS             36571..37581
                     /gene="hypE"
                     /locus_tag="JNY02_001332"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBB9432940.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37655..39733
                     /gene="flhA"
                     /locus_tag="JNY02_001333"
     CDS             37655..39733
                     /gene="flhA"
                     /locus_tag="JNY02_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBB9432941.1"
                     /translation="MSYTPMSGLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(39770..40123)
                     /locus_tag="JNY02_001334"
     CDS             complement(39770..40123)
                     /locus_tag="JNY02_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBB9432942.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40155..40334)
                     /locus_tag="JNY02_001335"
     CDS             complement(40155..40334)
                     /locus_tag="JNY02_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432943.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMFMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            40410..42971
                     /gene="mutS"
                     /locus_tag="JNY02_001336"
     CDS             40410..42971
                     /gene="mutS"
                     /locus_tag="JNY02_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBB9432944.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43077..43729
                     /gene="pphB"
                     /locus_tag="JNY02_001337"
                     /pseudo
     CDS             43077..43729
                     /gene="pphB"
                     /locus_tag="JNY02_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(43780..44547)
                     /locus_tag="JNY02_001338"
     CDS             complement(43780..44547)
                     /locus_tag="JNY02_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB9432945.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            44743..45651
                     /locus_tag="JNY02_001339"
     CDS             44743..45651
                     /locus_tag="JNY02_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBB9432946.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            45648..46910
                     /locus_tag="JNY02_001340"
                     /pseudo
     CDS             45648..46910
                     /locus_tag="JNY02_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            46907..>47520
                     /locus_tag="JNY02_001341"
     CDS             46907..>47520
                     /locus_tag="JNY02_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBB9432947.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
BASE COUNT        11239 a        12982 c        12373 g        10926 t
ORIGIN      
        1 gctacggatg caaatggcgg tgaggcgggg attcgaaccc cggatgcagc ttttgaccgc
       61 atactccctt agcaggggag cgccttcagc ctctcggcca cctcaccaca cgcctcttac
      121 gagtgcttcg aagaacttgt ttatcgctca tcgtcgctgc gtggcgcaca tattactttc
      181 tgagacttat aagtcaaaca atttttccca cacttttatc gtttgcacac ttcacgctca
      241 attagtctgt aaaaacgtca aaaagagtgt tttatctaca gaaaaacgga ggtctggcag
      301 atagcagtaa tgcaaaaaaa tggagactta agttgaatga acgggagtaa agcgaaaaga
      361 ctatagagtg aaggagaaat tttgagggtg cgtctcaccg ataaagatga gacgcggaaa
      421 gattagtaac tggactgctg ggatttttca gcctggatac gctggtagat ctcttcacgg
      481 tgaacagaaa cttccttcgg ggcatttacg ccaatacgta cctggttgcc ctttacccct
      541 aaaactgtca cggtgacctc atccccaatc atgagggtct caccaactcg acgagtcaga
      601 atcagcattc tttgctcctt gaaagattaa aagagtcggg tctctctgta tcccggcatt
      661 atccatcata taacgccaaa aagtaagcaa cgacaaacac attacatgta agcagtcatg
      721 gcattacatt ctgttaaacc taagtttagc cgatatacac aacttcaacc tgactttatc
      781 gttgtcgata gcgttaatgc gaatgccgtg aagcgagtcc acggcattgc ctgacgctta
      841 tattattgca atttcgcgct gacccagcct ttcacactgg ctaacgctgc aggtaaggcc
      901 gcagcatccg taccaccggc ttgcgccatg tcaggacgtc caccaccctt gccgcccacc
      961 tgctgagcga ccataccaat cagttcccct gctttcacac gatctgtgac gtccttagat
     1021 acgcctgcaa tcagagaaac cttaccttcg gctaccgttg ccagcacgat aattgtcgac
     1081 cccagctgat tttttaaatc gtcaaccatg gtacgcaaca ttttcggctc aacaccgcta
     1141 agctcgctaa ccaacagctt aacaccatta acatcaattg ccttactgga aagatttgcg
     1201 ctctcctgtg cggcagcttg ttctttaagc tgttgtagtt ctttttctaa ctgacgcgta
     1261 cgttccagta ctgagcgcac tttatccgcc agattattgc tatcgccttt cagcagatgc
     1321 gcgacttcgc ttaagcgatc gctgtctgca tgaacggtgg tgatagcgcc ttctccggtt
     1381 accgcttcga tacgacgaac gcctgcagca gtacccgatt cagagatgat gcggaacaga
     1441 ccaatatcac cagtgcggct ggcgtgagta ccgccacaca gctcggtgga gaaatcgccc
     1501 atgctcagca cgcgtacacg ctcatcatac ttctcgccga acagcgccat cgcacctttc
     1561 gctttcgccg cttcgagatt catgatgttg gtttcgatcg gcaaattgcg acgaatctgt
     1621 gcgttcacca ggtcttcgac cgcacgaatc tcttccggtt tcatcgcttc gttgtgtgag
     1681 aagtcgaagc gcagcacctt gtcgttaacc agtgaacctt tctgcgatac atgagtcccc
     1741 agaacctggc gcagcgcagc gtgcatcagg tgcgttgcgg agtgattcag acgaatacgg
     1801 gcgcgacgag cctcatcaat atcagcctgc acagcgtcgc ccactttcag agaacccgca
     1861 gcaagtttac cgatgtgacc aatcgcctgg ccgtatttct gcgtatcttc caccgcgaag
     1921 gagaagttag cgcctttcag ttcgccttta tcaccaacct gaccgccgga ttccgcatag
     1981 aatggcgttt gatccagcac gaccacagct tcctggcctg cattgatggc atcaaccgct
     2041 ttaccatcaa caaacagcgc ggtcactttg ccgttcagtt ccagatggtc atagccttta
     2101 aattcagatg cactatcaac acggatcatt gcgttgtaat cggcaccaaa gccgctggct
     2161 tcgcgcgcgc gacgacgctg ctcttccatt gctgcttcaa aaccagcttc gtcaactttg
     2221 atgttgcgct cacgacaaac atcagccgtc aggtcaaccg ggaagccata ggtgtcgtac
     2281 agacggaaag ccgtttcacc atccagcgta tcaccagaaa gttttgccag ctcttcatcc
     2341 agcaacgcca gaccgcgctc cagagtacgg gcaaactgct cttcttcagt cttcagcacc
     2401 tgctcaacct gcgcctgctg gcgtttcagg tcttcacccg cagagcccat aacgtcgatc
     2461 agcggaccaa ccagtttgta gaagaaggtt tctttcgcgc cgagcatatt gccgtgacgc
     2521 actgcgcgac gaatgatacg acgcagtaca taaccacggt tttcattgga cggcattacg
     2581 ccatccgcga tcaggaacgc acaagaacga atgtggtcag cgattacgcg cagcgattta
     2641 ttgctcagat cggtcgcgcc agtgactttc gctaccgcct ggatcaacgt gcggaacagg
     2701 tcgatgtcat agttagagtt aacgtgttgc agcaccgcag caatacgctc cagacccata
     2761 ccggtatcta cagacggctt cggcagcggt tccatcgtgc catctgcctg gcggttgaac
     2821 tgcatgaaga cgatgttcca gatctcaatg tagcggtcgc cgtcttcttc cggacttccc
     2881 ggagggccac cccaaatgtg gtcgccgtga tcgtagaaga tttcggtgca cgggccgcac
     2941 ggaccagtgt cacccatctg ccagaagttg tcagatgcgt atggcgcacc tttgttatcg
     3001 ccgatgcgaa taatacgttc gcgcgggatc ccgacttctt tttcccagat ttcgtaggct
     3061 tcgtcgtcgc tttcatagac ggtaacccac agacgctctt tcggcagggc aaaccatttt
     3121 tcgctggtca gcagttccca tgcaaactga atggcatcgt gtttgaaata gtcgccgaag
     3181 ctgaagttgc ccagcatttc gaagaaggta tggtgacgcg cggtgtaacc gacgttttcc
     3241 aggtcgttgt gtttaccacc cgcacgcacg cagcgttggg aagtggtagc gcgggaataa
     3301 ttacgcttgt cgagcccaag gaacacatcc ttgaactggt tcatcccggc gttggtaaac
     3361 aacaaagttg ggtcgttatg gggtaccagg gagctgctgg caactacctg atgtccctta
     3421 ctatggaaaa agtcgagaaa cgcctgacgg atctcagcgg tgctcttgct cataattatc
     3481 ctgaaatcaa gctaacgaaa tatcgccacc agctccagcg tgtcttaacc gccgggctgg
     3541 taactgaaaa gtgggaataa gataagtttt cttgactggg aagtaaaata ccgtatgcgt
     3601 tcagtcggca aaatttcgcc aaatatcctg gatatcttcc atcagatagc cacgatagag
     3661 cagaaaacgc tggatcttaa ctttttctga aaagacagtt ggcaaaggtt caccatattt
     3721 tcgcgtcgcc tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt
     3781 ttctgtcgct tcgcgggaaa tacctttctg attcagttcc tgacgaatac gtgcaggtcc
     3841 ataacctttg cggctacggc tggcgataaa gcgcgcaaca aatcggctgt catcgagata
     3901 gccatgttca tggcaccagg caataacgcg ctcgtaatct tctgccgtag catcaatctc
     3961 ttctgggcca tttttgccca taatcggtgc cgcgagttta cgtcgcagtt cttgctcact
     4021 gtgatcgcgc accgccagaa tgcgtaccgc acgatccaac aggcgagcat atgccgggcg
     4081 acgggatgtt gattctgtca tggcatatcc ttacaactta aaaaagcaaa agggccgcag
     4141 atgcgaccca ggttttgctg ataaacagat ttaatagtca atgagtatgc agaatgttcc
     4201 cgcaggccca attcgttaga ggatcgaggt gagagtaatt tttcacgcca gtgctggcct
     4261 gttcccctca ccctaaccct ctccccaaag gggcgagggg accgtccgtg cacacattat
     4321 ctattatcat caataacgaa cgattaaaaa tcttcgttag tttctgctac gccttcgctg
     4381 tcatctacag agaaatccgg cgttgagttc gggttgctca gcagcaactc acgtactttc
     4441 ttctcgatct ctttcgcggt ttccgggtta tctttcagcc aggcagttgc attcgcttta
     4501 ccctgaccga tcttctcacc tttgtagctg taccacgcgc ctgctttctc gatcagcttc
     4561 tcttttacgc ccaggtcaac cagttcgccg tagaagttga taccttcgcc gtagaggatc
     4621 tgaaattcag cctgtttaaa cggtgcagcg attttgttct tcaccacttt cacgcgggtt
     4681 tcgctaccca ccacgttttc gccctctttc accgcgccga tacgacggat gtcgagacga
     4741 acagaggcgt agaatttcag cgcgttacca ccggtagtgg tttccgggtt accgaacatc
     4801 acaccaattt tcatacggat ctggttgatg aagatcagca gcgtgttgga ctgcttcagg
     4861 ttacccgcca gcttacgcat cgcctggctc atcatacgtg ccgcaaggcc catgtgagag
     4921 tcgccgattt cgccttcgat ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg
     4981 ataacgtcta ctgcgccaga acgcgccagg gcgtcacaga tttccagtgc ctgctcgccg
     5041 gtatccggct gggagcacag caggttatcg atatcgacgc ccagtttacg tgcgtagatt
     5101 gggtccagcg cgtgttcagc atcgataaac gcacaggttt taccttcacg ctgcgctgcg
     5161 gcgatcacct gcagcgtcag cgtggtttta ccggaagatt ccggtccgta gatttcgacg
     5221 atacggccca tcggcagacc acctgcccca agcgcgatat ccagtgaaag cgaaccggta
     5281 gagatggttt ccacatccat ggaacggtct tcacccaggc gcatgatgga gcctttacca
     5341 aattgtttct caatctggcc cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata
     5401 gccattttta ctcctgtcat gccgggtaat accggatagt caatatgttc tgttgaaaca
     5461 attatactgt atgctcatac agtatcaagt gttttgtaga aattgttgcc acaaggtctg
     5521 caatacatac gcagtagcct gacgacgcac cgcatcacgg tcgccgctga agcattcccg
     5581 ccgggtaatg ccttcaccgc gggcagtggc aaaagcaaac cagacggtgc cgacaggctt
     5641 ctcttcactg ccgccatccg gcccggcgat accactaata gacacggcat aatcagcacg
     5701 agccgctttc agtgcaccta tcgccatttc caccacgacg ggttcactca ccgcgccatg
     5761 ctgcgccagc gtctcttcgc gtacgccgat catctgcgct ttggcttcgt tactgtaggt
     5821 gacaaatccg cgttcaaacc aggcggagct accggcaata tcggtaatca ctttcgctac
     5881 ccaaccaccg gtacaagact cggcagttgt tacggttgcg ccacgggctt tcagcgcctg
     5941 cccaacctgt tcgcttaact gcatcagttc actgtcagtc atcacaacct ctgtcagtca
     6001 aaaatttatg ccggacaaga tagcactttc gtcaggaaga tggtgttggc gttgcaattt
     6061 tacgaggagg attcagaaaa aagctgatta gccagaggga agctcacgcc cccctcttgt
     6121 aaatagctac tgtactcgcg ccagcgccac ggcttgtcct aactgccaga ccgccattgc
     6181 gtaatgggtg ctgtggttgt aacgggtgat ggtgtagaag ttcggcagac cgtaccagta
     6241 ctggtagccg gtgccaacat ccagacgcag caggctggct tgttgatggt tgcccagcgg
     6301 ctgctgtggc gttaaacctg cggcggcaag ctgcgaaatg ctgtacttag ttttgaaacc
     6361 atttggcaag cctggagcct gaccgtttgc cattatcgcg acctgatcgc ctttcaccca
     6421 gccgtgcgct ttgaaatagt tcgccacgct accgatcgca tcaaccggat cccacaagtt
     6481 gatatgcccg tcgccgctga aatctaccgc atattgtttg taagacgacg gcataaactg
     6541 tccgtagccc atcgccccgg caaaggaacc tttcagattg agcggatcgt cctgctcgtc
     6601 gcgcgccatc agcaggaagg tttccagctc gccagagaaa tactccgcgc ggcgtgggta
     6661 gttaaatgac agcgttgcca gcgcatcgag gatgcgagtt ttccccatca cgcgccccca
     6721 gcgggtttca acgccgataa tcccgacgat aatttccggc ggtacgccat acacctgcca
     6781 cgcgcgattc aacgcatctt catactgatt ccagaaaacc acaccgttct gcacgttgtc
     6841 cggcgtaata aattttttgc gataacgcag ccatgcgccg ttcggacctg atggcggttt
     6901 caccgatgtg gttggtgcct ggttatccat cagccgcagt accgaatcca gacgcttcgc
     6961 ctgggagaga atttcctgca actgctgacg atcgaaaccg tgtttgttca ccattttgtc
     7021 gatgaactgc tgggcattcg ggttattagc gaaatcgccg cccatctgca tcacattgtg
     7081 ctgcggctca agcaggaagc cgccagacgg cgttccggtg gtcgtttcag tctcagtagg
     7141 ttttggcttg ctgctacagg cggcaagcaa cacaaaaagg ggaagcaatg ttacataacg
     7201 acgcttgaac atgaggggtc catttaacag attcaaccag gggcaagtat ggtaaagcat
     7261 cacgccccgc acaaggaagc ggtagtcact gcccgatacg gactttacat aactcaactc
     7321 attcccctcg ctatactttt attcaaactt tcaaattaaa atatttatct ttcattttgc
     7381 gatcaaaata acacttttaa acctttcaac ctgattagat taggttgccg tttggtaata
     7441 aaacaataaa tcctgaagga gagaacaatg atagaaacca ttactcatgg tgcagagtgg
     7501 tttatcgggc tgttccaaaa gggcggagag gtgtttaccg gaatggtgac cggcattctt
     7561 ccgctgttga ttagcctgct ggttatcatg aacgcactga ttaattttat cggtcagcat
     7621 cgtattgaac gttttgctca acgttgcgcc ggtaaccctg tttcccgtta cctactgtta
     7681 ccgtgcattg gcacgtttgt cttttgcaat ccgatgacct taagcctagg tcgctttatg
     7741 ccggaaaagt acaaacccag ctactacgcg gcggcctctt atagctgcca ctcaatgaat
     7801 ggcctcttcc cccatatcaa ccctggcgaa ctgtttgttt atcttggcat tgccagcggt
     7861 ctgacaacgc tgaacctgcc acttggccca ctggcggtga gttatctgct ggttggtctg
     7921 gtcaccaatt tcttccgcgg ctgggtgacc gatctgacca ccgccatttt tgagaaaaag
     7981 atgggcattc aacttgaaca aaaagttcac ctggcaggag caacatcatg acgcgtattc
     8041 ggatcgaaaa aggaacgggt ggctggggcg gcccgcttga gctggaagct acgccgggca
     8101 aaaaaatcgt ctatatcacc gccggtaccc ggcctgcgat tgttgacaaa ctggcacaac
     8161 ttactggctg gcaggctatt gacggattta aagaaggtga acccgcggag gcggaaattg
     8221 gtgtcgcggt aatcgactgt ggcggcacat tacgctgcgg catctatccg aaacgacgta
     8281 ttcccaccat taatatccac tcgacgggca agtccggtcc gctggcgcag tacattgtgg
     8341 aagatattta tgtctctggc gtaaaagaag aaaacatcac tgtagtgggt gatgcgacac
     8401 cacaaccctc ttccgtgggc cgtgactatg acaccagtaa gaaaatcacc gaacaaagcg
     8461 atggtttact ggcgaagctg ggaatgggca tggggtccgc cgttgccgtg ttgtttcagt
     8521 ctggtcgtga caccatcgac actgtattaa aaaccattct gccgtttatg gcattcgtct
     8581 cggcgctcat tggcatcatt atggcttctg gccttggtga ctggattgcc cacggtcttg
     8641 ctccgctggc gagccatcca ctgggtctgg tcatgctggc gctcatctgc tccttcccac
     8701 tgctttcacc tttcctcggc ccaggcgcag ttatcgcaca ggttatcggc gtattgattg
     8761 gcgtgcagat tggtctcggc aatattccgc cgcatctggc tttaccggca ctgtttgcca
     8821 tcaacgcgca ggcggcctgc gacttcatcc cggtcggttt gtcgctggcg gaagcccgtc
     8881 aggacacggt tcgcgtcggt gtcccttctg tactggtgag ccgcttttta accggcgcgc
     8941 cgactgtact gatcgcctgg tttgtctccg gttttatcta tcaatagagg ctgaaacatg
     9001 accgttattt atcagaccac catcacccgt atcggcgcga gtgccactga cgccctcagc
     9061 gaccagatgc tcatcacctt tcgtgaaggc gcgccagcgg acctcgaaga gtattgcttc
     9121 attcattgcc acggcgagtt gaaaggtgca ctccatcccg gtttgcaatt ttcactcggg
     9181 cagcatcgct atccggtgac cgctgttggc agcgtggcgg aagacaacct tcgcgaactg
     9241 ggtcatgtca ccctgcgctt cgatggttta aacgaagcgg aatttccggg cactgtccat
     9301 gtggcaggcc ctgtacccga cgatatcgcg ccgggatcgg ttttgaagtt tgaatctgtt
     9361 aaggagtaaa aaatgaatca ggttgccgtt gtcatcggtg gtgggcaaac cttaggcgcg
     9421 ttcctgtgcc acggtctggc tgccgagggg tatcgcgtcg cggttgtcga tattcagagc
     9481 gacaaagccg caaatgtggc acaagaaatt aacgccgaat atggtgaagg tatggcgtac
     9541 ggttttggtg ctgacgccac tagcgagcaa agcgttctgg cgctctctcg tggggtagat
     9601 gaaatctttg gtcgcgtgga tttgctggtc tatagcgccg gaatagcaaa agcagccttt
     9661 atcagcgact tccagctcgg cgattttgac cgttcgctac aagtgaatct ggtgggttat
     9721 ttcctgtgtg cccgtgaatt ttcgcgtttg atgatccgcg acgggattca gggtcgcatt
     9781 attcagatca actcgaaatc cggcaaagtg ggcagcaaac acaactctgg ctacagcgca
     9841 gcgaaatttg gtggcgtcgg gctgactcaa tcactggcgc tggatctggc ggagtacggc
     9901 attacggtgc attcactgat gctcggtaac ctgctgaaat cgccgatgtt ccagtcactg
     9961 ttgccacaat acgcgaccaa gctgggtatc aaaccggatc aagtcgagca gtattacatc
    10021 gacaaagtac cgctcaaacg cggctgcgat tatcaagatg tgctgaatat gctgctgttc
    10081 tacgccagtc ctaaggcgtc gtactgcacc ggacagtcga tcaatgtcac cggcggtcag
    10141 gtgatgttct gatcaacagc ggagatccgt taaggatctc cgtgagacta tagaatgcct
    10201 gatgcgctac gctcatcagg catacaggac ttccgccact acattaagga aaagttatgg
    10261 tatccgcact catcaccgtc gccgttatcg cctggtgtgc gcaactggcc ttaggcggct
    10321 ggcaaatttc tcgttttaac cgtgccttcg acacactatg ccagcaaggg agggttggcg
    10381 tgggccgttc cagcgggcac tttaaaccgc gggtcgtggt cgccatcgcg ctggacgatc
    10441 agcagtgcat cgtcgacatc ttgtttatga aaggactgac tgtcttcgcc cgaccgcaaa
    10501 aaattcccgc aattaccggc atgcatgcgg gtgatttaca gcccgatgtg atctttcccc
    10561 atgatccact atcacagaat gctctatcat tggcgcttaa actgaaacgt ggataatttc
    10621 gttgtgaatg ttacttgctt gcgaagttat cattttgaaa cctaaatcag gtaatcacgc
    10681 ccatgaaacc tcgtcagcgt caggccgcca ttctggagta tctgcaaaag cagggtaaat
    10741 gctcggttga agaattggcg caatactttg acaccacagg cacaaccatt cgcaaagatc
    10801 tggtcattct ggaacatgcc ggaaccgtca ttcgtactta tggcggagtg gtgttgaata
    10861 aagaggaatc cgatccgcct atcgatcata aaacgctcat caacacccac aagaaagagc
    10921 tgattgcaga agctgccgtt agttttatcc atgatggcga ttcgatcatt cttgatgctg
    10981 gcagtaccgt tttgcagatg gttcccctgc tctcgcgctt taataacatc acggtgatga
    11041 ccaacagcct gcatatcgtc aatgcgctat ccgaactgga taacgaacaa actatcctga
    11101 tgccaggcgg aacatttcgc aaaaaatcgg cctcatttca cgggcaactg gcagagaatg
    11161 ccttcgagca tttcaccttc gataaattgt ttatgggcac cgacggcatc gatctcaatg
    11221 cgggcgtaac cacgttcaac gaggtttata ccgtcagtaa ggcaatgtgc aatgccgcgc
    11281 gcgaagtgat tttgatggcg gactcatcaa agtttggccg taaaagcccc aacgtagttt
    11341 gcagtcttga aagcgtcgat aagctaatta ccgacgcagg tatcgatccg gcgtttcgtc
    11401 aggcgctgga agagaaaggg atcgatgtga tcataaccgg agagagcaat gagtgaagca
    11461 ctactgaacg cgggacgtca gacgttaatg ctggagttgc aggaagcaag ccgtttaccg
    11521 gaacgtctgg gcgatgattt tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa
    11581 gtggtggttt cgggaattgg caaatcgggc cacattggta agaaaatcgc cgcaacactt
    11641 gccagtaccg gcacaccggc tttttttgtc catccggcag aagcactgca cggcgatctg
    11701 gggatgattg aaagccgcga tgtgatgctg tttatctctt actccggtgg cgcgaaggaa
    11761 ctggatctga ttattccgcg tctggaagat aaatctatcg cgctgctggc gatgaccggc
    11821 aaaccgacgt caccgctggg cctggcggcg aaagcggtgc tggatatctc cgtagaacgc
    11881 gaagcctgcc cgatgcacct tgcgccgacc tccagcaccg tcaataccct gatgatgggt
    11941 gacgcgctgg cgatggcggt catgcaggcg cgcggtttta atgaagaaga ttttgcccgc
    12001 tcccacccag ctggggcact gggcgctcgc ttgctgaata aagtgcatca tctgatgcgt
    12061 cgtgatgacg ctatcccaca ggtagcgtta accgccagcg tgatggatgc gatgctggaa
    12121 ctcagccgca ccggtctggg gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc
    12181 gtctttaccg acggcgattt acgtcgctgg ctggtaggcg gcggcgcact caccacgcca
    12241 gttaatgaag cgatgacgac aggcggcacc acattacagg cacaaagtcg cgccatcgac
    12301 gccaaagaag ttctgatgaa acgcaaaatc actgccgcac cagtggtaga tgaaaacggc
    12361 aaactcaccg gcgcaattaa cctgcaggat ttctatcagg ccgggattat ttaatccttc
    12421 aatcccagac gtttcgccag ccgatgcagg ttggcgacgt cagtttccag cattcgcgca
    12481 caggctgccc agttgtgatg attttgtgcc agtgcctggc gaatagtttc acgctggaac
    12541 gcttctgtcg cttcacgcag gttttgctta acaacgggca ccgccgccgc ttctggcggc
    12601 ggcaacgtca cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc atcgccgttg
    12661 cgggtggctc tcgccagcac taccacccga tgaatagcat gttccagttc gcgcacgttt
    12721 cccggaaaac tgtagtgttg cagtaaattt cgcgccccgg cacttaatac cacgcgggag
    12781 agcccctgcc gcaaacgaca ctgctcgcag aaataccccg ccagcagaat gacatcatcg
    12841 ccccgctcac gcagcggcgg caccgaaagt ggaaacacgc tcaggcgatg aaacaaatcg
    12901 gcgcggaatc gccctgccag cacctcttcg cgtaagtcgc ggttagtcgc cgccagcacg
    12961 cgcacatcga cccgcaaact acggtcatcg ccaacgcgct gaatatcgcc atactgcaac
    13021 accctcagca gcttggcctg caatgccaac gacaactcgc cgatctcatc gagaaacaga
    13081 gtaccgttat ccgccatttc aaacttcccg ctgcggttac tgatagcgcc agtaaacgct
    13141 cctttcacat gcccgaacaa ctcactttcc gccacacttt ccggcagtgc ggcacagttg
    13201 agatagacca gcggattcac cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc
    13261 tccttaccgg ttcccgtctc accgctgatc agaacgttga gatcggacgc cgccacaatc
    13321 tcaatctctt ttttcagttg cgtcatgcca ggggacaagc caatcatctg cgtctgtttc
    13381 accgcttcaa acggcgtggc atcgcctggc agcatattct ggctttccag ttgttcaatc
    13441 agcaacgcat tgcttaacgc tcccgccgcc agcgcggcaa tcagccgtaa ctcttcgtcg
    13501 ctgaaaacat cgaactgatc gggctgcatc ccgtcgagcg tcagtgcgcc gatcaggttt
    13561 tgtccgacaa acaatggcag accaacgcag gcgtgaacct tcagactctc ctgcccagga
    13621 atcaaaccgt catagggatc gggcaattcg ctgtctgcgg gaaagcgcac cacatccccg
    13681 gcgcgggcaa tcgcttccag ccgtggatgc ccttccagcg caaagcgtct accgagtaca
    13741 tcctttgcca gaccgtcgat ggcaagcgga ataaactgcc gcgaatcgta acgtagcaac
    13801 gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg aaaacgatcc
    13861 tgatgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc aacggaaaaa
    13921 ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc atttgattga
    13981 tagtcatttt gactactcat taatgagcat aattttattt atagagtaaa aacaatcaga
    14041 taagaaactg gcacgcaatc tgcaattagc aagacatctt tttagaacac gctgaataaa
    14101 ttgaggttgc tatgtctatt gtggtgaaaa ataacattca ttgggtgggt caacgtgact
    14161 gggaagtgcg tgattttcac ggcacggaat ataaaacgct gcgcggcagc agctacaaca
    14221 gctacctcat ccgcgaagaa aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc
    14281 gcgaatttgt gcagaacctg cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga
    14341 ttaaccatgc agaagaggac cacgccgggg cgctgaccga actgatggcg caaattcccg
    14401 atacgccgat ctactgtact gccaacgcta tcgactcgat aaatggtcat caccatcatc
    14461 cggagtggaa ttttaatgtg gtgaaaactg gcgacacgct ggatatcggc aacggcaaac
    14521 agctcatttt tgtcgaaaca ccaatgctgc actggccgga cagcatgatg acttacctga
    14581 caggcgacgc ggtgctgttc agtaacgatg ctttcggtca gcactactgc gacgagcatc
    14641 tgttcaacga tgaagtggat cagacggagc ttttcgagca gtgccagcgt tactacgcca
    14701 atatcctgac gccgttcagc cgcctggtaa cgccgaaaat taccgagatc ctgggcttta
    14761 acttaccagt cgatatgata gcgacctccc acggcgtggt ctggcgcgat aacccgacgc
    14821 aaattgtcga gctgtacctg aagtgggccg ctgattatca ggaagacaga atcaccattt
    14881 tctacgacac catgtcgaat aacacccgca tgatggctga cgctatcgcc cagggaattg
    14941 cggaaaccga cccacgcgtg gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg
    15001 aaatcctgac caatgtcttc cgctcaaaag gcgtgctggt cggcacttcg acgatgaata
    15061 acgtgatgat gccgaaaatc gctgggctgg tggaggagat gaccggatta cgcttccgta
    15121 acaaacgcgc cagtgctttc ggatctcacg gctggagcgg cggtgcggtg gatcgccttt
    15181 ccacgcgcct gcaggatgcg ggtttcgaaa tgtcgctcag cctgaaagcc aaatggcgac
    15241 cagaccagga cgctctggag ttatgccgtg aacacggtcg cgaaatcgcc cgtcagtggg
    15301 cgctcgcgcc gctgccgcag agcacggtga atacggtagt taaagaagaa acctctgcca
    15361 cgacgacggc tgacctcggc ccacgaatgc agtgcagcgt ctgccagtgg atttacgatc
    15421 cggcaaaagg cgagccaatg caggacgttg cgccaggaac gccgtggagt gaagtcccgg
    15481 ataacttcct ctgcccggaa tgctccctcg gcaaagacgt ctttgacgaa ctggcatcgg
    15541 aggcaaaatg agcaacggca ttgtgatcat cggttcgggc ttcgccgccc gccaactggt
    15601 gaaaaatatt cgcaaacagg acgccactat tccattaacc ctgattgccg ccgacagcat
    15661 agatgagtac aacaaacccg acctcagcca tgttatcagt caggggcaac gtgccgacga
    15721 ccttacccgc cagacggcgg gtgaatttgc cgggcagttt aatctgcgcc tgtttccgca
    15781 cacctgggtg acggatatcg atgccgaagc ccatgtggtg aaaagccaga ataatcagtg
    15841 gcaatacgac aagttagtgc tggcaaccgg tgccagcgcc tttgtcccgc ctgtgcctgg
    15901 gcgtgagtta atgctgacgt taaatagtca gcaagagtat cgcgcctgtg aaacgcaact
    15961 gcgggatgcc cgacgcgtgt tgattgtcgg cggtggtttg attggtagcg aactggcgat
    16021 ggatttttgt cgtgcaggca aagcggtcac gctaatcgac aacgctgcca gtattctggc
    16081 gtcattaatg ccaccagaag taagcagccg cttgcagcat cggttgacgg agatgggcgt
    16141 tcatctgctg ttgaaatctc agttgcaggg gctggaaaaa acggattctg gcattctggc
    16201 aacgctggac caccagcgta gtatcgaagt ggatgcggta attgccgcca ccggactgcg
    16261 cccggaaaca gccctggcac gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga
    16321 tagttatctg caaaccagta atgccgatat ttacgcgctg ggcgattgcg cggaaattaa
    16381 cggtcaggta ttgccgttcc tgcagccgat tcaacttagc gggatggtgc tggcaaaaaa
    16441 tcttctcggc aataacacgc cgctgaaact accggcgatg ctggtgaaaa tcaaaacgcc
    16501 ggaattaccg ctgcatctgg caggcgaaac ccagcgtcag gatttacgct ggcagattaa
    16561 taccgaacgc cagggaatgg tggcgcgcgg cgttgacgat gctgaccagc ttcgcgcctt
    16621 tgtggtcagt gaggatcgga tgaaagaggc atttgtattg ttgaaaacgt tgccggtgta
    16681 ggtgggctac tgtgcctaaa atgtcggatg cgacgctggc gcgtcttatc cgacctacgg
    16741 ggacgcatgt gtaggccgga taaggcattt acgccgcatc cggcaagggt gtccaaatgc
    16801 aacacgcttt atccgttctg gacttcaccc gctaaccaac gcgccgcagc aataaccccc
    16861 tgccccagag acaaaccgcc atcacccgcc ggtaaactct gtggaaagag caatgtgaaa
    16921 tcagcgagat aatgcgccag acgtgcacgc agcaaacggt tatgaataac cccgccgcta
    16981 aataccagcg tagtgatacc acgcatcgtg gcctgctcac gcatcaacgc ggcaaaaccc
    17041 tgcgccagcg catcatgaaa cgcccacgcg cgttgattaa ccggtgcctg ccagttcagc
    17101 cactgctgcc agaaagtggc gagatccagt tgattgtcca cccgcggcat cgtcaccgga
    17161 tgcgtcactc cgtcgcatga ggctgcgagc gcctccagag cacaagccgc ttcaccttca
    17221 taacttaacg tggctggcgc acagcccagt gccgccgcca ctgcatcgaa caaccgccca
    17281 cacgatgatg ccagcggcgc gttaattcca cgctcaatgg cccgcgccag tacgctccag
    17341 ttttgctgtt gcacacttgc cgtttcaggg taattctgcc actccggcac aaagcgcagg
    17401 cactgcgcca gcaggtttcg ccacggctgc ttcgccgcca aatcgccacc cggaagcgcc
    17461 actgcaggca agccgcccag gtgctcacat tcgcgatagt tcacccgcag gcactcgccg
    17521 ccccacaaag cgccattctc ccccataccg ataccgtcga gcgtcaaagc aatgacatca
    17581 ccgccatcca gcggccactg atgctctgcc agacacgccg ctgcatgggc atgatgatgc
    17641 agtaccgttt gcgtcggcag attcatttcg ctcgcccact ggctggagac atagcccgga
    17701 tgcacatcat gcacaatgta ttgcggagtg aaatcgtaga tgttttgcat caggcgtaac
    17761 gcttcgcgcc actgcatctg gatgccatcg tcacttaaat cgcccagatg ctgactcaac
    17821 accgcttgtt caccgcgcac caggcagaag gtatttttca gatccgcgcc aagacacagc
    17881 acaggcggaa catttttaaa gcccggaggc aaaaccagcg catccggcac atacccccgc
    17941 gaacggcgca gcatttcgcc gctttcgcgc accactgaat catccatccg ctgcacgatg
    18001 tcgcggttat gtagcaagaa tccgtcggca atgccctgca aatccgccag cgcctgttcg
    18061 ttgctgatag ctggtggttt accgctcagg ttgccggagg tcatcaccag cgggcattgc
    18121 agttcctgta acagcaaatg ctggagcggg ttcgcaggca acatcacccc gacttcgtta
    18181 aggccagggg cgatatcatc acaaagctca ggtacgtatt ttttatccac cagcacaatc
    18241 ggcgcagcgg gcgtggtaag caactggcgc gcagcgtctg gtaaactttc tgccactggc
    18301 aacatgactg ccagcggttt cgccgggcga tgtttgcgct cccgcagtgt cgccaccgcg
    18361 ttactgttac gtgcatcgca ggcaagatga aatccgccaa tccctttgat ggcgacaatg
    18421 ttgcccattt ttaactgtgc gatagctgcc tgtaatgccg cctcttgttc tgcatgttca
    18481 ccatgactta cccattcaag atgcgggcca cactccgggc aggccaccgg ctgggcgtgg
    18541 aagcgacgat cgagcgggtc gcggtactct ttgtcacagg ccggacatag cggaaacgcc
    18601 gccatcacgg taaacgggcg gtcgtaaggc atcgcgcgaa taatggtgaa acgcgggccg
    18661 cagtgggtac agttgataaa cggataacga taacgccgct cgcctggggt attcatttcg
    18721 gcaaggcaag cagggcaagt ggcggcatcc gggacaattt gcgtattcat ggtgccgccg
    18781 gtgctctggc ggatggtgaa ctcggtgggc agttgtgacc agataaacgg ctcacgctcg
    18841 acgctatcaa tacgcgccag tggcggacag tgctgatgca attgaacaag aaacgtttcc
    18901 ggatcttcca gcagccggac ttctacgcca tcgccgtcat tacatacatc gccgtgaaga
    18961 tttaattgct gtgccagctg ccagacaaac ggacgaaaac cgacgccctg cactttgcca
    19021 cgaatacgca gttggacacc gcaagatgtg ttttttgcca ttgagttatt cccgccatca
    19081 tgaattgcgt aacccgccct gccggacacg acagcgtcgt atccggcagt ctcaggtcgg
    19141 cgataccgcc cgctccgtat tctacgaata tttccgggaa ttcctttgat gccagaacag
    19201 ttctgtaaga tttttagaac atcagcgccg tacggcggcg tttttctgcg ctcagttgtt
    19261 caagtttatt acgatcgaca caaatcagcg catgggtcgg gcaagccgcc atacacgccg
    19321 ggccgtcttc acgatggttg cacaggtcgc atttattggc ttcggctttg tcagcccgta
    19381 cattcagacc cgcgccgctg ttgcgaatca caggacgtac caccacttcc atcgcgccat
    19441 acgggcaagc caccacgcag gttttgcaac caatgcaacg ttcctgcatc acatgaacaa
    19501 accctttatc acggctgata gcaccattcg ggcagacgtt agcgcacggt gcatcttcac
    19561 actgacggca aactgtcgcc gtggaaatgt tcacaccttt aatgacatgg atacgcggta
    19621 aaaatgtttc cggggtcagc gatgcacagt cctgattttc ctgatgagaa accacgcacg
    19681 ccacttcaca ggtacggcaa ccaatacatt tactcgcgtc agcaatgatg aaacggttca
    19741 tcaaattctc cagcaatgac agttaatgcg ccgatacatt cacaaatcat gccagttttt
    19801 aatttgctgt tatttaagga aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca
    19861 ctagtgacga tgacatgtga tgacaatgtt tatcgcgaaa gggcaatgag tgagtcgcgg
    19921 cggatcagtt ttccgctaaa ggttttcggc ggtgagaaat ccccgccatc gagcataaaa
    19981 atcagccgtc caataatttc ctgaatcatc tcagtcaccg gaatttttac gctggagagc
    20041 gccggaacga tatagggggc aattgcgata tcatcgaatc cgataactga cacctgctct
    20101 ggcaccgcta cgccgcgctc gtgcaacgct ttgatcgcac ctatcgccat atcgtcgtta
    20161 ctggcaacta acgcgctaaa tttagcccca cgttcgagca acgtttctac cccttcggcc
    20221 ccgctggcgg gcgtccattt accgttagcg ataagttttt cattgagcgc aataccatgc
    20281 tgcgacagcg cgtctttata cccggcaaga cgttcaatgc tggtggggga atccatcgag
    20341 ccggtaagga aagcaatctc ctgatgcccg gcgtttatca actctgccac ggcgttaaag
    20401 ctggtctgtt tatgatcgca ccagacgcta tggctgctat tattgcgcag gcggcgatta
    20461 agcaccatta tcggctgact gtgcgcgtca atgatgtcat caatctcatc cacgcttaaa
    20521 aaacgcgggt aaatcataat ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg
    20581 cgctcttctt cggcgctgtg tttaccatct gccaatagca actgtcgccc tttctcttcc
    20641 gccattcgcg cggcatgaaa cagtaattca ctaaaataaa tgccgtgata aagcgtgttg
    20701 gtcactacca gccccagcgt ctgagtactc ttcgccgaca gattgcgggc cagcaaattt
    20761 gggcggtaac tgctctcttc taccgcctga aacacgcgat ctttagtctc ctggctgacg
    20821 tagccattac ctgaaagcac gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc
    20881 acttccagca tcgtcgtcat cattttcatc cctttacacg caatcaacgc agtgtactgc
    20941 accgtttgcc gattgtcctt gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac
    21001 aaatataaaa aatgaacaat taactctctt gcttatttag tgacaactat tcatgatttt
    21061 gtgaaaccgg tttcttaatt ccgtttcagc atcggtattt ttccgtcacg tcgactgata
    21121 acaactacat ctaccctact gataacagga taaaatccga tggccaaaaa ttatgcggcg
    21181 ctggcacgct cggtgatagc ggcactgggc ggcgttgata acatctcggc agtcacgcac
    21241 tgtatgacgc gtttgcgctt tgttatcaaa gatgaccagc ttatcgacag cccgacgtta
    21301 aaaaccatcc ccggcgtgct cggcgtggta cgtagtgaca accagtgtca ggtaattatc
    21361 ggcaatacgg tttcgcaggc ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag
    21421 cccgcacagc ccgtgggtaa acccaaactc acgctgcgtc gcattggtgc ggggatcctc
    21481 gatgcgctga tcggcaccat gtcaccgctg atcccggcga ttatcggcgg atcgatggtc
    21541 aaactgctgg cgatgatcct cgagatgagc ggcgtgctga caaaaggatc gccgacctta
    21601 accattctga atgtgattgg tgacggtgct ttcttcttcc tgccgctaat ggtcgcggca
    21661 tctgccgcca tcaaatttaa aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg
    21721 gtacatccga gttttattga attaatggcg aaagcggccc agggtgaaca tgtcgaattt
    21781 gccctgatcc cggtcaccgc ggtgaaatac acctacacgg tgatcccggc gctggtgatg
    21841 acctggtgcc tgtcatatat cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa
    21901 aacttcctca agccaatgct gattgtgttg attgccgcac cgctggcaat cctgctgatt
    21961 ggcccgattg gtatctggat cggtagcgcc atttcggcgc tggtttacac cattcatggt
    22021 tatctgggct ggctttcagt cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc
    22081 gggatgcacc gcgtctttac gccaaccatc attcagacca ttgccgaaac cggcaaagaa
    22141 ggaatggtca tgccatcaga gatcggcgct aacctgtcgc tgggcggttc atcactggcg
    22201 gtgccgtgga aaacgaaaaa cccggaactg cgccagacgg cgctggctgc ggcggcatca
    22261 gccattatgg cggggatttc cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt
    22321 ccgcttatcg ccagtcttat cagcggtttt atttgcggcg cggttgccgg tatggcgggg
    22381 cttgccagcc actcaatggc agcgccgggg ctatttacca gcgtgcaatt cttcgatccg
    22441 gcgaatccaa tgagcatcgt ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg
    22501 tttatcctca cactgttgct cggctttgag gatattcctg ttgaggaagc ggctgcccag
    22561 gcgcgaaagc atcagagcgt acaaccgacc gtcgccaaag aagtaagtct taattgagga
    22621 tgaaaatgtc agtatttcca gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt
    22681 ctgaaggtgc gttccgtgaa ggcggaaaag ggctgaccac cgtcgatatg atcccacacg
    22741 gcgagcatcg aatggcggtg aaactggggc tggaaaaacg ttttcagttg cgcgatgacg
    22801 agttttatcc cagccatgag gcgacggatt tttatcatcg ttataaagaa gatatcgccc
    22861 tgatggcaga gatgggattc aaggttttcc gtacctcaat tgcctggagc cgtctctttc
    22921 cgcaggacga tgaaatcacg cccaatcagc agggcattgc tttttatcgt cctgtctttg
    22981 aagagtgtaa aaagtacggt atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga
    23041 tgcatctggt caccgaatat ggctcctggc gtaaccgcaa gctggtggag tttttcagcc
    23101 gctacgccag aacctgcttt gaagcatttg atggtctggt gaaatactgg ctaaccttca
    23161 atgaaatcaa cattatgttg catagcccgt tctccggcgc gggtctggtg tttgaagaag
    23221 gtgaaaatca ggatcaggtg aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc
    23281 tagccaccaa aatcgcccat gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg
    23341 gcggtaactt ctacccttac agctgcaaac cggaagatgt ctgggcggcg ctggagaaag
    23401 atcgggaaaa cctgtttttt atcgatgtgc aggcgcgcgg cgcgtatccg gcttactctg
    23461 cccgcgtatt tcacgaaaaa ggggtaacca tagataaagc gctgggcgat gatgagatcc
    23521 tgaaaaacac cgtcgatttt gtctctttca gctattacac ctcgcgctgc gcctcggcgg
    23581 agatgaacgc caacaacagc agtgcggcga acgtggtgaa atcgctgcgt aacccgtatc
    23641 tgcaagtgag cgactggggc tggggaattg atccactcgg tctgcgtatc accatgaata
    23701 tgatgtacga tcgttatcag aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag
    23761 atgaacttgc tgccaatggc gagattaacg atgactatcg catcagctat ttacgcgaac
    23821 atatccgcgc aatgggcgaa gcgattgcag atggcattcc gctgatgggc tacaccacat
    23881 ggggctgtat tgatttagtt tccgcctcta cgggtgaaat gagcaaacgc tacggttttg
    23941 tctacgtaga ccgtgacgac gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt
    24001 tctggtggta taaaaaagtg attgccagta atggggaaga tttagagtaa gtaacagtgc
    24061 cggatgcggc gtgaacgcct tatccggcct acatagttcg tgcgacatgt aggcccgata
    24121 agcgcagcgc atcgggcaat ttagcatttg gcattagtca aaaatcaaag aaaccattcc
    24181 ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg atatcgtcag ggatcgttgt
    24241 ccagacaaaa tctgactgac cagggattta gagccgattt cgttcttcaa atccgcatag
    24301 gtcagattat gttgatcaat cagcgtgcga agtaacgcca cgccaacagg cattgccgca
    24361 acggctttgt cgaactctgc aaactcttgg ttgttgtttt catattctgc aatgcggctc
    24421 gccagaaagt cgataagtgg attggtgtca tcttcttcaa tcaaatattc gaccagttcc
    24481 agtgcttcac gataatcatc ttctgagtca ctacctccca gaaaggggac tgcattgacc
    24541 agttctcttg ttgctttaac agcgtgtgca gcattagcag tcattctttg ttctctctgt
    24601 agtaacgtgt cagtttgtca tactcggcgt gattagttat gtgcttcaca aagaaccgct
    24661 tattaacaaa gtttatgtag gcaatcaccc ctaggttgtt cccacctaca tccagaacgt
    24721 accatttgtt gcgatattta aaattgtcga ggttgggaaa cgccgttcgc atctcttctg
    24781 gcgtagaaaa atccgtttcc ttaataaccc gatacaatgc ctgaagagcc agagcgtcgt
    24841 taggatattt acgcgcagac tcttcaaatg gcgctttaga aatgatgtgc atttttaagt
    24901 tttaacttcc ttatagtaag taaacagcca catctgcgag ttctatcctt atgtgttcgg
    24961 aaactatagc tcatcaaatc caagttcaca atatgtaaac acttgtgcgc gatataatta
    25021 cccccaaaat cctcatagcc actctgcaaa aagtcagttc tggttcaccc ctcaagcaca
    25081 tcccccttta tcaagcaacg ctgcgtctgc gccagatctt ccgtttgcgg gtcgcgcccg
    25141 gcgatgggtt tgaggacgag tttacggttg ggggtataag taaaacgcgt gagcgcggga
    25201 tagcttaact tgtcaaacca gccgtgaaca cgctgccatt tggcgtcttt tacctgatac
    25261 agactggtta tccagtaacc agaattaaac tgcatatcca gcaattgcgt gtgaccattg
    25321 ccttgcaaat ccagcaaatc atctacgcca gttttgcttg cggtgtagaa tcccctgggt
    25381 tcaaagacac agggacggcc ttcgcgcgtg aacgtcataa gaattaaatg tgcataagga
    25441 gccagaccat taccggtact tggtaaccag ataaccagat cctgaatgcc gttacgatca
    25501 agatcggcgc gatagagttg ccctttgccc gctatgtttg ccagcatacc cacaggcaca
    25561 gtccaggatt taccggcgcg gtctttagcg gttaattgcc agtaatcttc ttcatttggc
    25621 atcttctcaa gcgtcacgcc agcaacgccc actttcctaa aatgccaggt ttgctcggca
    25681 tcataaggca tggtcaggag aggtttcagc gcccgctttt gcgcgtattc gctgtcactc
    25741 attggcagat attcggtaaa gctggtggcc tgtgcaggca gcactaagct caataaaaaa
    25801 gcgcacatcc ctgttctgat gttcaattcc ctgaattccc tgaattccct gatggagaat
    25861 tattcttctt ccaccgctaa ctgcgcaaat ccaccatttc cttcccagcc ttccagtcgt
    25921 tgataaacgg tttctaccgc atctttaatc ggctgggtca tcgggtagta aaagccgacg
    25981 atatccggct gaatgccgag gaaaatcact tcaccaatgt cttcttttaa ctggtcgata
    26041 aggtaattca acggcatgtt atgggtagtc atcataaaca tctcggcgat atcatccggg
    26101 tcgatgatgc ggatttcgcc ggggtttagc cccatatccg tggcgtcgac aatcagcagt
    26161 cgtgtcgggc gcagttcacg gatggcgacg atgtcgtttt ccggtgcact accgccgtca
    26221 atcaccaccc agttaccttt cggcgcggcg gcgcactttt ccgccagcag cgggccagcg
    26281 ccatcatcgc ccatcatgct attgccaaca cagagtaaaa cgtcagtcac gcagtctcct
    26341 caccatcaaa tagatggcgt tttcctgatg aatatcatgc agcatactta acagcattgt
    26401 actccattcc tgctgctgcg aagtttgcgc tgcccgggct ttatcgaatg cgttggccag
    26461 catcggcaca tggttaatgt cgatgacgat ctcgccatat ttcggtacgc cttccatttt
    26521 gtggcgggct tcgctgcccg cctccagagt ggcaatccat gccagatatt catcccatgg
    26581 gcaggtgagc gccgcttcca ggcaatcgat aaccccaagg tggtgaccaa tcgccaggct
    26641 gtaatagacc acctgctgcg cctcggcggg cgtggcatcg ttctcatcaa taaatttacg
    26701 gctcagttga ctgaacacca ccttttcact catcggatac gcgcctcttc aacaacatga
    26761 ttcagatggc tgacaatctc gttcagacgc ggatcgtttt ccgcttccag ccagcgtgca
    26821 acctgttctt cgccctgccc taactgtgta aggtaatcat cggcaatctg acgaccgtaa
    26881 cgataacccg ccaggcgacg tgcttcgcga tccactttca cgcgcagcgg ctgcaccata
    26941 tcgccatgca ggatctccgc cggttgttca tccagttcac ccggcccacg ggcgtgaatt
    27001 ttctgctcca gcaggccgag cgccattgca aagccgtaca gcgtggcggc aggcgttggc
    27061 gggcagctag ggatataaac atccacaggg acaattttat ccgtaccgcc ccacacgcag
    27121 tagagatcgt gtaagatccc gccgctgtta ccgcaggcac cgtatgagat acagattttc
    27181 gggtccggcg cggactgcca cgcacgcagc gcaggggatc gcattgcacg ggtgaccgcg
    27241 ccggtaaaca gtaaaatatc cgcatgacgc ggtgaaggaa cgactttaat gccgaagcgt
    27301 tctgcatcga acagcggaga aagcgtggcg aaaatttcga tttcgcagcc gttgcagccg
    27361 ccgcagtcca cgcggtaaac ataggcagaa cgtttgattt ttttcagtaa cgacgccttc
    27421 atgctggcga tggattcatc caccgtcatc gggaccggaa tgccgttggc gtcacggggg
    27481 cctaataaat tgctcatcag atggcctctt tcatatggcg agtcagttca atacggtcgg
    27541 acggcaccag gcatttctgg cgcttacatt ccgggcaagt ctcaaagctt tcgcggtggt
    27601 tttccgcgcg gctgtcacca ttgtgcttaa gcagcgcaat ggcgtagtcg atctctttct
    27661 gtacggcgaa aggacgattg cagacgcggc agttgcacag cgcgaagcgg gactgttgca
    27721 ggaagtcttc tttcttccac accgccagtt cgtactcttg cgacagtttg atcgccgccg
    27781 tcgggcaaac ttcttcgcag cgtccacaga agatgcagcg cccgagatta aactcccacg
    27841 ccagctctcc tgtggcgagg tcagtttcaa ccgttaaggc gtttgacggg caggcattga
    27901 cgcaagccgc gcagccgatg cactgctgcg ggttctgctc tggcttacca cggaagtttt
    27961 tatcaaccgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    28021 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    28081 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    28141 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    28201 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    28261 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    28321 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    28381 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    28441 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    28501 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    28561 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcaggcg atttccgggt
    28621 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    28681 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    28741 ctttcagcag atcgtgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    28801 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    28861 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gagtgcaggc
    28921 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    28981 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    29041 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    29101 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    29161 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    29221 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    29281 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    29341 catccggcag accaaccgga atcaaaccgt acatatcgcg cacttcgcgc tcgccccaca
    29401 ccgccgccgg aacgcgcggc gtcacggacg gatactccgg tttgttggcg tcaacttcga
    29461 cgcgaaccgt tacccaacac ttggtgccct tctccatcga cagcacgtaa taaacggcgt
    29521 aatgaccatt cagtttgcgt tcgtcgttac caaacagcac cgacagccag ccaccctgct
    29581 tgtagtagag aaactccacc acttccggca gatagttcac ctttacggtg acagtcagct
    29641 gatctttggt ctgccaggcg tggtccagca cgacgcctgg aaatgcctca ttcagcgcgg
    29701 cgagataatg ttgacctaat ttttcttcag acatgctcaa actctcttta atcacgccgc
    29761 cagcaaggag acgaacgcta aaaatgcaaa gccaaaccca gcccaggtaa tgcgcggagt
    29821 aatatcaaga cgcagacggg ccatactgtt ttcgaacagc gcgataacca ggacgccgac
    29881 caccagtttg acgatggcaa tcaccagcgc cagcagcagt ccaccgacgg tgaaggtttc
    29941 catttgtccc cacggaataa acaccccgac gaacatctgc aacaccacca gctgtttcag
    30001 gctgataccc catttcatga cgccaaagcc gctaccgctg tattcggaga gcggaccttc
    30061 ctgcaactcc tgctcggctt ccgccaggtc gaacggcagt ttgcccattt cgataaaagt
    30121 ggcgaacgca caggcgcaga gcgccagcac cagcgggatg ctttggctca gcggccagtg
    30181 ataaacggtg tcggtaatgt tgctgatgtt ggtggaaccg gcaacctgtg cggcaaccca
    30241 cagaccaagc agcaacatcg gttcgaccag cacgccaagc atcgcttcac ggctcgcacc
    30301 gatagcggta aacgggctac cggtatccag accggaaatg gcaaagaaga aacgcgcgat
    30361 ggcgaagaga tacagtaagg tgatcaaatc gcccagttgc ggcagcggag aaccgatagt
    30421 cagcatgacg cccaccatca catacggcgt caggcggaac atccagccag aggcatccgg
    30481 gccaacgctc tggcggccca gcagtttgat aatgtcgcga tactcctgca acacgcccgg
    30541 cccgcgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga gcagcggcgc
    30601 aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc tcaggctcct
    30661 cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg gcggaacagc
    30721 aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag ccatttgcgc
    30781 agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg agcggtaatc
    30841 accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga acgtgacggc
    30901 aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca ggcaatcagc
    30961 agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg cagaggtaca
    31021 gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac gcagcaaatc
    31081 gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt ttcggcttct
    31141 ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca catcaccgcc
    31201 agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg ggcaacaaac
    31261 gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc ggcaaacccg
    31321 ttcagtggcg gcagcgcagc cattgccatc aacccgacta acatggcgat ggagataacc
    31381 ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt acggaaccag
    31441 acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag cagatggtac
    31501 agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat acccgttacg
    31561 ccagcgccca gccccagccc cagccccagc cctagcccta gccctagccc tagccctagc
    31621 cctagcccta gccctagcag gataatgccg atattttcca gggtgtggta agccagcagg
    31681 cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt gatcatgccg
    31741 agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag tgacagggtt
    31801 aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc agctggcgca
    31861 gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc agccagcagg
    31921 ccaaagccaa tcacaccgag cagccagata tcggaaccga gcagccagat atcggaaccg
    31981 agcggcagct gttgcatacg catatccagc aggcgcagat ccagcgtgcc ataacgctgc
    32041 cacagcagcc agcaagcaat caccagcagc agagtgccaa gacgccccag cgcaaaccac
    32101 agtttgccct ctttgctgtt gctggtgagg aacaccgcac acaaggccat gacttcggcc
    32161 attaccacga acatgccgag gttgctggcg atgacggcgc agacggcggc agccatcaac
    32221 atattgatct gcaagccgtt gcacttcacc tgcgcgtggc gatgccagtc aatgttatag
    32281 aggctgacaa acagaccgca aaggccgagg gtaatcagcc aaatcgcgtt aagcggggag
    32341 atttgcacat cgtagcttac cagcgacatc gcaccgctca cgccaaccga gccagtcagt
    32401 acagtgaagc ccgcggctgc cgtatacaga ctaccaaccg cgccgccaat tccggctatc
    32461 cagccactta gcgctttttg aaaagaaaag agaaatgcca gaacagcggc ggcgacaaac
    32521 catgccacgc cgctattgat cagggaaatt gcgctcattt agcctctcca ctttgagcct
    32581 gctgaaacaa ggtgagatcg ccaaagtccg tgttaaaagt cagctcacgc ttacgtttgc
    32641 tgacgcgggc gatatcggtg ttatccacca gatgcagggc tttagtcggg cacatccgca
    32701 cacaggccgg accttgttca tcaaagctac aaaggtcaca tttgacggcg atcgcgcgaa
    32761 tacctggcac ccagtcaagc aatgtgctga cacgcgccgg agcaggcggt gccggtggcg
    32821 ctttcggggt attggcgttt gccggaatat ccagcggacg gctgccggaa aattcaattg
    32881 cgccaaacgg gcaggcgatg ccgcacagtt tgcagcttac gcacagactt tcattcaact
    32941 gcacggcccc atcaacgcgg gtgatggcgt taaccgggca gaccaccgcg cagggtgcat
    33001 cttcacagtg gtgacagagc agcggcgcag attctttttc attgagcatc actctcaggc
    33061 gcggcattga ttgcaggccg tgctggcgat gcgtctctga acaggcggcc tcacaggtgt
    33121 ggcagccgat acagagcgtg gagtcggcaa ttacaaaacg attcaccagg cattcctcag
    33181 gtgattgtca tttttgacga aaacatgccg atgaaatgtc attttcgaca ctcatcgaca
    33241 cgcccatccc caaacaggcg taacgcctgc aaaacgggca aagcctcagc tcatgctgcc
    33301 gggctttgtc cctttaccag ttggcttaaa ttcaccggca cattgttttc aatggcggtg
    33361 tataagctgt cgtaaacgcc agcgattttt tcgagatagt gttccagaac ctgttcgcga
    33421 tcgcggatgt tgaccttgcc atcaatcatg ccgtcaatgc tcagttgcgc ctgcgcaatc
    33481 cgctgtttgt cttcgccatc ttttgtttcg acgtaatact cgatggtgtg gctgttaaag
    33541 ccatccgcca gggtgacgta aatacgaaaa tgttcaaaaa ggacgaaaca gagttctttg
    33601 tccggcgcgc agctcatggc gtgatgcagg cgcgctttcg ataacgtgat cccctgaacc
    33661 agcgaattgc agtagatgtg ccactggtcc tgtaggcgac gatgccgctg tgcgatgtaa
    33721 tcggctttct cgcttatttc ccaaatagtc attgtcaggt tactcgttta acagagatgc
    33781 cagctttaag cattttttgt accaactttt aatttattgt tattaaagag atttttaagc
    33841 taaagatgaa tttcgtcgcc gtgtcgacgt gtcatttcga catcatcgac attattcacc
    33901 gcagggataa tcaacactgg cgcaattatt gcttgtagct ggcaatagtt aatgggaggc
    33961 gatatgcacg aaataaccct ctgccaacgg gcactggaat tgatcgaaca gcaggccgca
    34021 aaacacggcg caaaacgcgt aactggggtc tggctcaaaa ttggcgcatt ttcttgtgtc
    34081 gaaaccagct ctcttgcctt ttgttttgat ctggtttgcc gcggcagcgt ggcggaaggt
    34141 tgtaaactgc acctcgaaga acaagaggcc gaatgctggt gtgaaacatg ccaacagtat
    34201 gtgacgctac tgacccagcg cgtccgccgc tgtccacagt gtcatggtga catgctacag
    34261 attgtggcag acgacggttt acagattcgg cggatagaaa tagaccagga gtgagcgatg
    34321 tgtacaacat gcggttgcgg tgaaggcaac ctgtatatcg agggtgatga acataaccct
    34381 cattccgcgt ttcgtagcgc gccatttgcc ccggcggcac gcccgcagat gaaaatcacc
    34441 ggcattaaag cgcctgaatt tacccccagc cagactgaag aaggcgacct gcattacggt
    34501 catggcgaag cgggcactca cacaccgggc atgagccagc gtcggatgct ggaagtcgaa
    34561 attgacgtgc tggacaaaaa caaccgtctg gctgaacgca accgcgcgcg ctttgctgcc
    34621 cgcaagcaac tggtgctcaa cctggtttcc agccctggtt ccggtaaaac caccctgctg
    34681 acggaaacct taatgcgcct gaaagacagc gttccgtgcg cggttattga aggcgaccag
    34741 caaaccgtga acgatgccgc acgcattcgc gctaccggta caccagcgat tcaggtgaac
    34801 accggtaaag gctgccatct tgacgcacag atgattgccg acgccgcacc gcgtctgcca
    34861 ctggacgata acggtattct gtttatcgaa aacgttggca acctcgtatg cccggccagc
    34921 ttcgatctcg gtgaaaaaca caaagtagcg gtgctttccg ttaccgaagg tgaagacaaa
    34981 ccgctgaaat atccgcatat gtttgccgcc gcctcgctga tgctgctcaa caaagttgac
    35041 ctgttgccgt atctcaactt tgacgttgag aagtgcatcg cctgcgcccg cgaagtcaat
    35101 ccagaaattg aaatcatcct tatttccgcc accagcggcg aagggatgga ccagtggctg
    35161 aactggctgg agacacagcg atgtgcatag gcgttcccgg ccagatccgc accattgacg
    35221 gcaaccaggc gaaagtcgac gtctgcggca ttcagcgcga tgtcgattta acgttagtcg
    35281 gcagctgcga tgaaaacggt cagccgcgcg tgggccagtg ggtactggtt cacgttagct
    35341 ttgccatgag cgtaattaat gaagccgaag cacgcgacac tctcgacgcc ttacaaaaca
    35401 tgtttgacgt tgagccggat gtcggtgcgc tgttgtatgg cgaggaaaaa taatgcgttt
    35461 tgttgatgaa tatcgcgcgc cggaacaggt gatgcagtta attgagcatc tgcgcgaacg
    35521 tgcttcacat ctctcttaca ccgccgaacg ccctctgcgg attatggaag tgtgtggcgg
    35581 tcatacccac gccattttta aattcggcct cgaccagtta ctgcctgaaa acgttgagtt
    35641 tatccacggt ccgggttgcc cggtgtgcgt actgccgatg ggtagaatcg acacctgcgt
    35701 ggagattgcc agccatccgg aagtcatctt ctgtaccttt ggcgacgcca tgcgcgtacc
    35761 agggaaacag ggatcgctgt tgcaggcaaa agcacgcggt gccgatgtgc gcatcgttta
    35821 ctcgccgatg gatgcgttga aactggcgca ggagaatcca acccgcaaag tggtgttctt
    35881 cggcttaggt tttgaaacca ccatgccgac caccgccatc actctgcaac aggcgaaagc
    35941 gcgtgatgtg cagaattttt acttcttctg ccagcacatt acgcttatcc cgacgctgcg
    36001 cagtttgctg gaacagccgg ataacggtat cgacgcgttc ctcgcgccgg gccacgttag
    36061 tatggttatc ggcactgatg cctataattt tatcgccagc gattttcatc gtccgctggt
    36121 ggtcgctggt ttcgaacccc ttgatctact gcaaggcgtg gtcatgctgg tggagcagaa
    36181 aatagcggcc cacagcaagg tagagaatca gtatcgtcgg gtggtaccgg atgccggtaa
    36241 cctgctggcg caacaggcga ttgccgatgt gttctgtgtc aacggcgaca gcgaatggcg
    36301 cggcttaggc gtgattgaat cttctggcgt gcacctgacg ccggattatc aacgattcga
    36361 tgccgaagca catttccgcc cggcaccgca gcaggtctgc gatgacccgc gcgcgcgttg
    36421 tggtgaggta ttaacgggca aatgtaagcc gcatcaatgc ccgctgtttg gtaacacctg
    36481 taatcctcaa accgcatttg gtgcactgat ggtctcctcc gaaggagcgt gcgccgcgtg
    36541 gtatcagtat cgtcagcagg agagtgaagc gtgaataata tccaactcgc ccacggcagc
    36601 ggcggccagg cgatgcagca attaatcaac agcctgttta tggaagcctt tgccaacccg
    36661 tggctggcag agcaggaaga tcaggcacgt cttgatctgg cgcagctggt agcggaaggc
    36721 gaccgtctgg cgttctccac cgacagttac gtcattgacc cgctgttctt tcctggcggt
    36781 aatatcggca agctggcgat ttgcggcacc gcgaatgacg ttgcggtcag tggcgctatt
    36841 ccgcgctatc tctcctgtgg ctttatcctc gaagaaggat tgccgatgga gacactgaaa
    36901 gccgcagtga ccagcatggc agaaaccgcc cgcacggcag gcattgccat cgttactggc
    36961 gatactaaag tggtgcagcg cggcgcggca gataaactgt ttatcaacac cgctggcatg
    37021 ggcgcaattc cggcgaatat tcactggggc gcacagacgc taaccgcagg cgatgtattg
    37081 ctggttagcg gtacactcgg cgaccacggg gcgactatcc ttaacctgcg tgagcagctg
    37141 gggctggatg gcgaactggt cagcgactgc gcggtgctga cgccgcttat tcagacgctg
    37201 cgtgacattc ccggcgtgaa agcgctgcgt gatgccaccc gtggtggtgt aaacgcggtg
    37261 gttcatgagt tcgcggcagc ctgcggttgt ggtattgaaa tttctgaatc agcgctgccg
    37321 gttaaacctg ccgtgcgcgg cgtttgcgaa ttgctgggac tggacgccct gaactttgcc
    37381 aacgaaggta aactggtgat cgccgttgaa cgcaacgcgg cagagcaagt gctggcagcg
    37441 ttacattccc atccactggg gaaagacgcg gcgctgattg gtgaagtggt ggaacgtaaa
    37501 ggtgttcgtc ttgccggtct gtatggcgtg aaacgaaccc tcgatttacc acacgccgaa
    37561 ccgcttccgc gtatatgcta ataaaattct aaatctccta tagttagtca atgacctttt
    37621 gcaccgcttt gcggtgcttt cctggaagaa caaaatgtca tatacaccga tgagtggtct
    37681 cggacagcaa gggttgttcg acatcactcg gacactattg cagcagcccg atctggcctc
    37741 gctgtgtgag gctctttcgc aactggtaaa gcgttctgcg ctcgccgaca acgcggctat
    37801 tgtgttgtgg caagcgcaga ctcaacgtgc gtcttattac gcgtcgcgtg aaaaagacac
    37861 ccccattaaa tatgaagacg aaactgttct ggcacacggt ccggtacgca gcattttgtc
    37921 gcgccctgat acgctgcatt gcagttacga agaattttgt gaaacctggc cgcagctggc
    37981 cgcaggtggg ctatacccca aatttggtca ctattgcctg atgccactgg cggcgggagg
    38041 gcatattttt ggtggctgtg aatttattcg ttatgacgat cgtccctgga gcgaaaaaga
    38101 gttcaatcgt ctgcaaacat ttacgcagat cgtttctgtc gtcaccgaac aaatccagag
    38161 ccgcgtcgtc aacaatgtcg actatgagtt gttatgccgg gagcgcgata acttccgcat
    38221 cctggttgcc attaccaacg cggtgctttc ccgcctggat atggacgaac tggtcagcga
    38281 agtcgccaaa gaaatccatt actatttcga cattgacgat atcagcattg tcttacgcag
    38341 ccaccgtaaa aacaaactca acatctactc cactcactat cttgataaac aacatcccgc
    38401 ccacgaacag agcgaagtcg atgaagccgg aaccctcacc gaacgcgtgt tcaaaagtaa
    38461 agagatgctg ctgatcaatc tctacgagcg ggacgattta gccccctatg aacgcatgtt
    38521 gttcgacacc tggggcaacc agattcaaac cttgtgcctg ttaccgctga tgtctggcga
    38581 caccatgctg ggcgtgctga aactggcgca atgcgaagag aaagtgttta ccactaccaa
    38641 tctgaatttg ctgcgccaga ttgccgaacg tgtggcaatc gctgtcgata acgccctcgc
    38701 ctatcaggaa atccatcgtc tgaaagaacg gctggttgat gaaaacctcg ccctgaccga
    38761 gcagctcaac aatgttgata gtgaatttgg cgagattatt ggccgcagcg aagccatgta
    38821 cagcgtgctt aaacaagttg aaatggtggc gcaaagtgac agtaccgtgc tgatcctcgg
    38881 tgaaactggc acgggtaaag agctgattgc ccgtgcgatc cataatctca gtgggcgtaa
    38941 taatcgccgc atggtcaaaa tgaactgcgc ggcgatgcct gccggattgc tggaaagcga
    39001 tctgtttggt catgagcgtg gggcttttac cggtgccagc gcccagcgca ttggtcgttt
    39061 tgaactggcg gataaaagct ctctgttcct cgacgaagtg ggcgatatgc cactggagtt
    39121 acagccgaag ttgctgcgtg tattgcagga acaggagttt gaacgtctcg gcagcaacaa
    39181 aatcattcag acggacgtac gtttaattgc cgcgactaac cgcgatctga aaaaaatggt
    39241 cgccgaccga gagttccgta gcgatctcta ttaccgcctg aacgtattcc cgattcacct
    39301 gccgccacta cgcgagcgtc cggaagatat tccgctgctg gcgaaagcct ttaccttcaa
    39361 aattgcccgt cgtctggggc gcaatatcga cagcattcct gccgagacgc tgcgcacctt
    39421 gagcaacatg gagtggccgg gtaacgtacg cgaactggaa aacgtcattg agcgcgcagt
    39481 attgctaaca cgcggcaacg tgctgcagct gtcattgcca gatattgctt taccggagcc
    39541 tgaaacgccg cctgccgcaa cggttgtcgc ccaggagggc gaagatgaat atcagttgat
    39601 tgtgcgcgtg ctgaaagaaa ccaacagcgt ggttgccggg cctaaaggcg ctgcgcaacg
    39661 tctggggctg aaacgcacga ccctgctgtc acggatgaag cggctgggaa ttgataaatc
    39721 ggcattgatt taactgcaaa ttgccggaca gatctgcctg tccggcatac tattcacgag
    39781 gttttttcgg acgatatttt tccggcagtt ctggcaccgg acgcttgtca tcgatgagat
    39841 gacgcacggt taagatcgga tgacgccaca gcattctcgg cccggcccaa cgcataatct
    39901 gtttcatctc ttcacgcttt gcaggctggt aacagtgcac cggacactgc ttacaggctg
    39961 gtttctcttc gccgaacaca catttatcca gccgcttttg cgcgtaaaca aacaacgcct
    40021 cgtaatgctc cggctccgct gacgcctgcg ggcatttcgc ttgataaaga tcgatcattt
    40081 ttttaatcgt cagtttttca cgagagatac gcttgccgga catgctgcct ccacctcatt
    40141 aagatgcatt tatattacat cttaatctta aagggcacta tgactctaaa gaagaagggt
    40201 tcgccaatcg atacaatttt gcgtacttgc ttcataaaca tcacgcaaaa gctgcaaaac
    40261 agcatctttc ccggaaccag catcaagaac tcgccgttcg cttcttcccc tgaaatgatt
    40321 aactccggta tcatgtgcgc cttatgtgat tacaacgaaa ataaaaacca tcacacccca
    40381 tttaatatca gggaaccgga cataacccca tgagtgcaat agaaaatttc gacgcccata
    40441 cgcccatgat gcagcagtat ctcaagctga aagcccagca tcccgagatc ctgctgtttt
    40501 accggatggg tgatttttat gaactgtttt atgacgacgc aaaacgcgcg tcgcaactgc
    40561 tggatatttc actgaccaaa cgcggtgctt cggcgggaga gccgatcccg atggcgggga
    40621 ttccctacca tgcggtggaa aactatctcg ccaaactggt gaatcaggga gagtccgttg
    40681 ccatctgcga acaaattggc gatccggcga ccagcaaagg tccggttgag cgcaaagttg
    40741 tgcgtatcgt tacgccaggc accatcagcg atgaagccct gttacaggag cgtcaggaca
    40801 acctgctggc ggctatctgg caggacagca aaggtttcgg ctacgcgacg ctggatatca
    40861 gctccgggcg ttttcgcctg agcgaaccgg ccgaccgcga aacgatggcg gcagagctgc
    40921 aacgcactaa tccggcggaa ctgctgtatg cagaagattt cgccgagatg tcgctgattg
    40981 aaggccgtcg cggcctgcgc cgtcgcccgc tgtgggagtt tgaaatcgac accgcgcgcc
    41041 agcagttgaa tctgcaattt ggcacccgcg atctggtcgg ttttggcgtc gagaacgcgc
    41101 cgcgcggact ttgtgctgcc ggttgtctgt tgcagtatgc gaaagatacc caacgcacga
    41161 ccctgccgca tattcgttct atcaccatgg aacgtgagca ggacagcatc attatggatg
    41221 ccgcgacacg tcgtaacctg gaaattactc agaacctggc gggcggtgcg gaaaatacgc
    41281 tggcttctgt gctcgactgc accgtcacgc cgatgggtag tcgtatgctg aaacgctggc
    41341 tgcatatgcc agtgcgcgat actcgcgtgt tgcttgagcg ccagcaaact attggcgcat
    41401 tgcaggattt caccgccgag ttgcagccgg tactgcgtca ggtcggcgac ctggaacgta
    41461 ttctggcacg tctggcttta cgaactgctc gcccacgcga tctggcccgt atgcgccacg
    41521 ctttccagca actgccggag ctgcgtgcgc agttagaaac tgtcgatagt gcaccggtac
    41581 aggcgctacg tgagaagatg ggagagtttg ccgagctgcg cgatctgctg gagcgagcaa
    41641 tcatcgacac accgccggtg ctggtacgcg acggtggtgt tatcgcatcg ggctataacg
    41701 aagagctgga tgagtggcgc gcgctggctg acggcgcgac cgattatctg gagcgtctgg
    41761 aagtccgcga gcgtgaacgt accggcctgg acacgctaaa agttggcttt aatgcggtgc
    41821 acggctacta cattcaaatc agccgtgggc aaagccatct ggcacccatc aactacatgc
    41881 gtcgccagac gctgaaaaac gccgagcgct acatcattcc agagctaaaa gagtacgaag
    41941 ataaagttct cacctcaaaa ggcaaagcac tggcactgga aaaacagctt tatgaagagc
    42001 tgttcgacct gctgttgccg catctggaag cgttgcaaca gagcgcgagc gcgctggcgg
    42061 aactcgacgt gctggttaac ctggcggaac gggcctatac cctgaactac acctgcccga
    42121 ccttcattga taaaccgggc attcgcatta ccgaaggtcg ccatccggta gttgaacaag
    42181 tactgaatga gccatttatc gctaacccgc tgaatctgtc gccgcagcgc cgtatgttga
    42241 ttattaccgg cccgaacatg ggcggtaaaa gtacctatat gcgccagacc gcactgattg
    42301 cgctgatggc ctacatcggc agctatgtac cggcacaaaa agtcgagatt ggacctatcg
    42361 atcgcatctt tacccgcgta ggcgcggcag atgacctggc ttccgggcgc tcaaccttta
    42421 tggtggagat gactgaaacc gccaatattt tacataacgc caccgaatac agtctggtgt
    42481 taatggatga gatcgggcgt ggaacgtcca cctacgatgg tctgtcgctg gcgtgggcgt
    42541 gcgcagaaaa tctggcgaat aagattaagg cattgacgct gtttgccacc cactatttcg
    42601 agctgaccca gctaccggag aaaatggaag gcgtcgccaa cgtacatctc gatgcgctgg
    42661 agcacggcga cactatcgcc tttatgcaca gcgtgcagga cggcgcggcg agcaaaagct
    42721 acggtctggc ggttgctgct ctggcgggcg taccgaaaga ggttattaag cgcgcacggc
    42781 aaaaactgcg tgagctggaa agcatttcgc cgaacgccgc cgcgacgcaa gtggatggta
    42841 cgcagatgtc tttgctgtca gtgccagaag aaacttcgcc tgcggtcgaa gcactggaaa
    42901 atctcgaccc ggattcactg actccgcgtc aggcgctgga gtggatttat cgcttgaaga
    42961 gtctggtgta ataacaattc ccgatagtct tttgctatcg ggaatattaa cgacaactga
    43021 cgaatcaaat aaaaataccc tgtataatag gaaagcttat tttacagggt aaaaccatgc
    43081 catctacacg ctatcaaaaa atcaatgccc atcactatcg ccatatatgg gtcgttggtg
    43141 atattcatgg tgaatatcag ttattacaat cccgcttaca tcaactctct tttttccccg
    43201 aaaccgactt acttatttct gtcggcgata atattgatcg tgggccggag agtcttgacg
    43261 tcctgcgcct gctaaaccag ccctggttta tctccgttaa aggcaaccac gaagcaatgg
    43321 cgctggatgc gttcgcgacc ggcgatggca atatgtggct tgccagcggt ggtgactagt
    43381 ttttcgattt aaatgattgc aacaagaggc aatagatctg ttgctgaaat tccatcacct
    43441 tccacatatt attgaaatca ctaacgacat aataaaatat gtcatcgcac atgcagatta
    43501 tccgggtgat gaatatctct ttggcaaaga aatagcggag agcgaattac tctggcctgt
    43561 ttatcgtgtg cagaaatcgc ttaatggcga gttacaacaa ataaacggcg ctgattattt
    43621 tatatttgga catatgatgt ttgataacat tcagacgttc gctaaccaga tttatattga
    43681 taccggatcg ccgaaaagtg ggcggctgtc attttataaa ataaggtagt ctcatgcttc
    43741 ttctgtgaag catgagtaac cagatgttat tgcaggccat tattcatttt tcgctaccag
    43801 caaagagaga tcctgcttcg ccagcgcgcg actggcactc tccggcaaac cgtcgtctgt
    43861 aataatctga tcaaactcgc ttaatggtaa cgccagccat gtcgccacct gaccatattt
    43921 cgtcgcatca cagaccaaaa ctcgctggcg gctagcactg gcaatcgccc gtttcaccgt
    43981 gactttatct tccgctggcg tagaaatccc ccgcacactc catgacgatg cagaaataaa
    44041 agcctgatca atcatcaggc tgcgcagcat ggtcgcagcg gcttccccga cacaggaacg
    44101 gttttcccga cacactgcac cgccagtgtg aataattgtg caattactgt tgtcgagcaa
    44161 gtagttcgca ataacgaaat cgtttgtgac cacagtcagt gactccatgt gaatcagatg
    44221 ctgtgctatc gctaacgtgg tcgttcccgc atccagatag atacaacttc ccggctgaac
    44281 aagacttgcc gccagcttgc caatagccgc tttttgcgtc attgccagcg cagtttttac
    44341 ctgatgagaa ggttcatgcg ccacgcgtcc cggagactgg acgcctccgg acaccagcac
    44401 aacggctccc tgctgctcca gtttttgtaa atcccgacga atggtcatat gtgacacatt
    44461 aattctgtcc gttagttcag caatactgac aatgcctttt tcagctacca tctcaaggat
    44521 gatttggcga cgctctacgg gtatcaactt ttgctccttc ctttgtcctg ctgacattct
    44581 acgctatttg cctgcgaaac gtgcgcggcg caactaacgc ttagttcaca taaaataaca
    44641 caaaatgtta atttatgtga atcagctcac catatcgtta tctcccagcg cttatattca
    44701 caatgtcaaa caaaatatca cttaaattaa caaggagagc agatgaaaac gggatctgag
    44761 tttcatgtcg gtatcgttgg cttagggtca atgggaatgg gagcagcact gtcatgtgtc
    44821 cgcgcaggtt tttctacctg gggcgcagac ctgaacagca atgcctgcgc tacgttgaaa
    44881 gaggcaggtg cttgcggggt ttctgataac gccgcgacgt ttgccgaaaa actggacgct
    44941 ctgctggtgc tggtggtcaa tgcggcccag gttaaacagg tgctgtttgg tgaaacaggc
    45001 gttgcacaac atctgaaacc cggtacggca gtaatggttt cttccactat cgctagtgct
    45061 gatgcgcaag aaattgctac cgctctggct ggattcgatc tggaaatgct ggatgcgcca
    45121 gtttctggtg gtgcagtaaa agccgctaac ggtgaaatga ctgtcatggc ctccggtagc
    45181 gatattgcct ttgaacgact ggcacccgtg ctggaagccg ttgccggaaa agtttatcgc
    45241 ataggtgcag aaccgggact aggttcgacc gtaaaaatta ttcaccagtt gttagcgggc
    45301 gtacatattg ctgccggagc cgaagcgatg gcacttgcag cccgtgcggg gatcccgctg
    45361 gatgtgatgt atgacgtcgt gaccaatgcc gccggaaatt cctggatgtt cgaaaaccgg
    45421 atgcgtcatg tggtggatgg cgattacacc ccgcattcag ccgtcgatat ttttgttaag
    45481 gatcttggtc tggttgccga tacagccaaa gccctgcact tcccgctgcc attggcctca
    45541 acagcattga atatgttcac cagcgccagt aacgcgggtt acgggaaaga agacgatagc
    45601 gcagttatca agattttctc tggcatcact ctaccgggag cgaaatcatg atcaagattg
    45661 gcgttatcgc cgatgatttt accggcgcga cggatatcgc cagttttctg gtggaaaacg
    45721 gtctaccaac ggtacaaatt aacggtgttc caacaggtaa aatgccggaa gcaatcgacg
    45781 cactggtgat cagcctgaaa acgcgctcct gtccggtggt tgaagccaca cagcaatcgc
    45841 tggcggctct gagctggttg caacagcaag gttgcaaaca gatctatttc aaatacagct
    45901 ctactttcga cagtacggcg aaaggtaata ttggcccggt taccgatgca ttaatggatg
    45961 ctctcgacac gccgtttacg gtcttctctc cggccctgcc ggtcaacgga cgtacggttt
    46021 atcaggggta ttttttcgta atgaatcaac tgctggccga atccgggatg cgccatcacc
    46081 cggtaaatcc catgaccaac agctatcttc cccgtctggt tgaagcgcaa tccacagggc
    46141 gctgcggcgt cgtttcggca catgttttcg aacaaggtgt ggatgccgtt cgtcaagagc
    46201 tggctcgctt acagcaagag gactaccgct acgcggtgct tgatgcgctg accgaacacc
    46261 atctggaaat tcagggagaa gccttgcgcg atgccccact ggtaacgggc ggttctggtc
    46321 tggcgattgg cctggcccgg cagtgggcac aagaaaacgg taaccaggct cgcgaagcag
    46381 ggcgtccgct cgctgggcgc ggcgtagtgc tctccggttc atgctctcaa atgaccaacc
    46441 gctaggtggc acattaccgt caaattgcac cagcccgtga agttgatgtg gcacgctgcc
    46501 tctcaaccga aactctggcc gcttatgcac acgaactggc agagtgggtt ctgggccagg
    46561 aaagtgtact tgctccactg gtttttgcca ccgccagcac tgacgcattg gcagcaattc
    46621 aacagcaata cggtgcacaa aaagccagtc aggcagtaga aactctgttt tctcaactag
    46681 cggcgcggtt agcagcggaa ggcatgacac gctttattgt cgcaggtggt gagacctccg
    46741 gcgtagtcac acagagcctg ggcataaaag ggtttcatat tggcccaacc atttcccccg
    46801 gcgtgccgtg ggtaaacgca ctggataagc ctgtctcact cgcccttaaa tctggcaact
    46861 tcggtgatga agcctttttt tcacgagccc aaagagagtt tttatcatga gcgatttcgc
    46921 aaaagtagag cagtctttgc gagaggagat gacgcggatt gccagttcat tctttcagcg
    46981 cggctatgca accggttcgg ctggcaatct gtcgctgctt ttacctgacg ggaatttact
    47041 ggcgacaccg acaggttcat gcctgggcaa tctcgatccg cagcggcttt ccaaagtcgc
    47101 cgcggatggc gaatggttaa gtggtgacaa accctcgaaa gaggtgctct ttcatctggc
    47161 gctgtatcgc aacaatccgc gctgtaaagc ggtggtgcat ttgcacagca catggtcgac
    47221 ggcgctttcc tgcctgcaag ggctggacag cagcaacgtt attcgtccgt tcacaccata
    47281 cgtggtgatg cggatgggaa atgtcccgct ggtgccctat taccgaccgg gcgataaacg
    47341 catcgcacag gatctggcgg aactggcagc agacaatcag gcttttttac tggcaaatca
    47401 tggcccagtg gtttgcggtg aaagcctgca agaagccgcc aacaatatgg aagagctgga
    47461 ggaaacggca aagctgattt ttattctcgg tgaccgcccg atccgttatc tgaccgcagg
//
LOCUS       DADWTH010000023        44112 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.023, whole genome shotgun sequence.
ACCESSION   DADWTH010000023 DADWTH010000000
VERSION     DADWTH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.023"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="JNY02_001342"
     CDS             complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="JNY02_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBB9432948.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVAFAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="JNY02_001343"
     CDS             complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="JNY02_001343"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBB9432949.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5526..5930)
                     /locus_tag="JNY02_001344"
     CDS             complement(5526..5930)
                     /locus_tag="JNY02_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBB9432950.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="JNY02_001345"
     CDS             complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="JNY02_001345"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBB9432951.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIV"
     gene            complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="JNY02_001346"
     CDS             complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="JNY02_001346"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBB9432952.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="JNY02_001347"
     CDS             complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="JNY02_001347"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBB9432953.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9966..10750
                     /gene="glcC"
                     /locus_tag="JNY02_001348"
                     /pseudo
     CDS             9966..10750
                     /gene="glcC"
                     /locus_tag="JNY02_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            complement(10874..12016)
                     /locus_tag="JNY02_001349"
                     /pseudo
     CDS             complement(10874..12016)
                     /locus_tag="JNY02_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12420..14108
                     /locus_tag="JNY02_001350"
                     /pseudo
     CDS             12420..14108
                     /locus_tag="JNY02_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14105..15019
                     /locus_tag="JNY02_001351"
     CDS             14105..15019
                     /locus_tag="JNY02_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBB9432954.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15052..15300
                     /locus_tag="JNY02_001352"
     CDS             15052..15300
                     /locus_tag="JNY02_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB9432955.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15300..16472
                     /locus_tag="JNY02_001353"
     CDS             15300..16472
                     /locus_tag="JNY02_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBB9432956.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16508..17587)
                     /gene="lptG"
                     /locus_tag="JNY02_001354"
     CDS             complement(16508..17587)
                     /gene="lptG"
                     /locus_tag="JNY02_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBB9432957.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17584..18654)
                     /gene="lptF"
                     /locus_tag="JNY02_001355"
     CDS             complement(17584..18654)
                     /gene="lptF"
                     /locus_tag="JNY02_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBB9432958.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18685..19245)
                     /locus_tag="JNY02_001356"
     CDS             complement(18685..19245)
                     /locus_tag="JNY02_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBB9432959.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19257..20081)
                     /locus_tag="JNY02_001357"
     CDS             complement(19257..20081)
                     /locus_tag="JNY02_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432960.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20081..21430)
                     /locus_tag="JNY02_001358"
     CDS             complement(20081..21430)
                     /locus_tag="JNY02_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBB9432961.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNIVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21476..22234)
                     /locus_tag="JNY02_001359"
     CDS             complement(21476..22234)
                     /locus_tag="JNY02_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432962.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22266..22979)
                     /locus_tag="JNY02_001360"
     CDS             complement(22266..22979)
                     /locus_tag="JNY02_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBB9432963.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23153..23845
                     /locus_tag="JNY02_001361"
     CDS             23153..23845
                     /locus_tag="JNY02_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBB9432964.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23894..25393)
                     /gene="pitB"
                     /locus_tag="JNY02_001362"
     CDS             complement(23894..25393)
                     /gene="pitB"
                     /locus_tag="JNY02_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBB9432965.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGVNDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25685..27544)
                     /gene="gss"
                     /locus_tag="JNY02_001363"
     CDS             complement(25685..27544)
                     /gene="gss"
                     /locus_tag="JNY02_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBB9432966.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27749..28615
                     /gene="yghU"
                     /locus_tag="JNY02_001364"
     CDS             27749..28615
                     /gene="yghU"
                     /locus_tag="JNY02_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBB9432967.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGNEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28738..28986)
                     /gene="hybG"
                     /locus_tag="JNY02_001365"
     CDS             complement(28738..28986)
                     /gene="hybG"
                     /locus_tag="JNY02_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBB9432968.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29120..29461)
                     /gene="hypA"
                     /locus_tag="JNY02_001366"
     CDS             complement(29120..29461)
                     /gene="hypA"
                     /locus_tag="JNY02_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBB9432969.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29454..29942)
                     /gene="hybE"
                     /locus_tag="JNY02_001367"
     CDS             complement(29454..29942)
                     /gene="hybE"
                     /locus_tag="JNY02_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBB9432970.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29935..30429)
                     /locus_tag="JNY02_001368"
     CDS             complement(29935..30429)
                     /locus_tag="JNY02_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBB9432971.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30429..32132)
                     /gene="hybC"
                     /locus_tag="JNY02_001369"
     CDS             complement(30429..32132)
                     /gene="hybC"
                     /locus_tag="JNY02_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBB9432972.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32129..33307)
                     /gene="hybB"
                     /locus_tag="JNY02_001370"
     CDS             complement(32129..33307)
                     /gene="hybB"
                     /locus_tag="JNY02_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBB9432973.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33297..34283)
                     /gene="hybA"
                     /locus_tag="JNY02_001371"
     CDS             complement(33297..34283)
                     /gene="hybA"
                     /locus_tag="JNY02_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBB9432974.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34286..35404)
                     /gene="hybO"
                     /locus_tag="JNY02_001372"
     CDS             complement(34286..35404)
                     /gene="hybO"
                     /locus_tag="JNY02_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBB9432975.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPYNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35593..35880)
                     /locus_tag="JNY02_001373"
     CDS             complement(35593..35880)
                     /locus_tag="JNY02_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBB9432976.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35999..36886)
                     /locus_tag="JNY02_001374"
     CDS             complement(35999..36886)
                     /locus_tag="JNY02_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBB9432977.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37043..38083
                     /gene="gpr"
                     /locus_tag="JNY02_001375"
     CDS             37043..38083
                     /gene="gpr"
                     /locus_tag="JNY02_001375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBB9432978.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38123..38617)
                     /locus_tag="JNY02_001376"
     CDS             complement(38123..38617)
                     /locus_tag="JNY02_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBB9432979.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38808..39692
                     /gene="yghA"
                     /locus_tag="JNY02_001377"
     CDS             38808..39692
                     /gene="yghA"
                     /locus_tag="JNY02_001377"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBB9432980.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     ARLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39964..40389)
                     /gene="exbD"
                     /locus_tag="JNY02_001378"
     CDS             complement(39964..40389)
                     /gene="exbD"
                     /locus_tag="JNY02_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBB9432981.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40396..41130)
                     /gene="exbB"
                     /locus_tag="JNY02_001379"
     CDS             complement(40396..41130)
                     /gene="exbB"
                     /locus_tag="JNY02_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBB9432982.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41382..42569
                     /gene="metC"
                     /locus_tag="JNY02_001380"
     CDS             41382..42569
                     /gene="metC"
                     /locus_tag="JNY02_001380"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBB9432983.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRDLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVVRVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42709..43368
                     /locus_tag="JNY02_001381"
     CDS             42709..43368
                     /locus_tag="JNY02_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBB9432984.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43408..>44112)
                     /gene="yqhC"
                     /locus_tag="JNY02_001382"
     CDS             complement(43408..>44112)
                     /gene="yqhC"
                     /locus_tag="JNY02_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBB9432985.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10943 a        11766 c        10845 g        10558 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagcttgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taaattaatg
      541 attttttcat ttcttcgcgc ctaataaaag tattcaaatt cagatgattt ttctgctcaa
      601 cagacacaaa caggcaataa tactctccaa agcgaacaat atttggcatt acgatttaat
      661 gcttcataat caccaaatta tttaaccata aattttctta tttctaacaa aatgattggt
      721 gtgttgttgg agttaaaata catacataat attaacatct gccaacattt cactttccgc
      781 aagacggatg atttcgaatc ctcatccacg ttaccctatc ccaaatatct ttattaatag
      841 gcagtaacga acactgttat agcaagatta acttattaac ctaacggcat cttttatgta
      901 acaagtggag ataaattgct taaaattgat cctcgacaaa tcttaaaagt tctcattatt
      961 ctgcaaaatc agctttgcga ataatcttat gtttttggga gttttgggaa ttttgatcta
     1021 ctcattgttc tagtgaaaaa tataaataag agaaaaaacg ccattcgtca tgaaagcaac
     1081 agatgaagca atgtaataac atactaaaat tgatctcatg aaagtgggta ctttataacc
     1141 agcacagaag ccaagttgta taaatattaa acaattcgca tcgtgaaacg gagactacgt
     1201 ataaagaagc caatcgtaaa aacggttccc tggcgagata aacgacagac aaatggaaag
     1261 ccgggtgata tttaccttca tccggcttta ttgatttacg agactaacat cccggtaaac
     1321 acatacgcct gcagcagggt gataacgccg ataacgctgg caaaaatcag actgtgcttc
     1381 acggtgtagc ggaacagttc agattctcgg cccaccatgc ccgtcgcggc gcaggccacg
     1441 gcgatagatt gcggggagat catcttgcca gttacgccgc cgctggtatt tgctgccacc
     1501 agcagagtgt cagagacgtt gatttgctgc gccgtggtcg attgcagtga accaaacagg
     1561 gcgtttgagg aagtgtccga cccggtaagg aatacgccca gccagccgag aaacggtgag
     1621 aaaaacggga acatcacgcc tgtacctgcc agtaccagcg ccagcgtggt ggacatgccg
     1681 gaatagttgg tgacgaaggc gaatgccagc accatgccaa tcgacaatat cggccacttc
     1741 agactaatta gcgtttcggc aaagacgcca atccctttct tgatgcccac gccgaggatg
     1801 aagatagaga taatcgccgc aataaaaata gcggtgccgc cagccgagag aggttcgaat
     1861 ttaaacaccg catccattgg cgttggttgg gcgacaatgg gtgccgcttt caacacttgt
     1921 tgatgcaaat gagggatctg gaaattaatc accagtgaat aaaacgcgcc gcccggagca
     1981 aataacgctt taaacggctt catggtccag atggtgacca gcaccgttaa gattaaaaac
     2041 ggtgaccacg cacgaatgat ttgccccaga ctatattctg aaggcacggg accgccagaa
     2101 gatggcttat ttaccaccat cgcacctgcg gattgcccca tgctgattgc cgtttcggta
     2161 tttttcggcc gccagacttt aaggaataaa gcgagtgaga cgatactcac cagcgccgaa
     2221 gtaatatccg gcagttccgg accaatatag ttagaggtaa agaactgagt gacagcgaag
     2281 cttcccccag caaccagcgc cgctggccac gtctctttca cccctttcca gccgtccatc
     2341 attgctacca gccagaacgg cacaagaacc gacaggaacg gtaactgacg tcccgccatt
     2401 gcgccaatgt ggaacggatc gattcccgtt acctgaccgg cgaccagaat cggcacgccc
     2461 aacgcaccaa acgccaccgg cgcagtattg gcaatcagac acagccccgc cgcgtataac
     2521 ggtttgaagc ccaggcccac cagcagcgca ccggtaatcg ccaccggcgc accaaagcca
     2581 gccgctcctt ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga
     2641 tcgtcggtga tggagataac cgagctgcgg ataatatcga actgcccgct ggcaacggtt
     2701 aatttataca ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata
     2761 aagccatagc ccgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca
     2821 atcaggatag ataatataag ggttattgct ccagcgacat gtcctttcag acgtaatacc
     2881 gcgaatgcaa cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt
     2941 cctcccatcg gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct
     3001 ttatatgctt gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata
     3061 ggcgttatac tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa
     3121 gtatatcggg taagggtttc tgttccgcac acgcaggcgc agagtatcgt taagatgtcc
     3181 atattgttgt tttaggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg
     3241 ataatcgctc cggttatttc cgggataaat gtactaccgc agttactatc atagccccga
     3301 caataaaact tgccggggct tttttgacgc tattaatgac tttctttttc gcgtaaacgc
     3361 caggcgtgta ataacggttc ggtataaccg tttggctgtt tcacgccgag gaagattaaa
     3421 tcgctggcag ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg acgataagcc
     3481 ggatcgccag cattttgctg atcaaccact ttcgccatat tctccagcga cgcctgcacc
     3541 tgttctttgg tcagaatacc gtgacgtaac cagttggcga tatgctggct ggagatacgc
     3601 agcgttgcgc ggtcttccat cagcgccacg ttgtgaatat ccggcacttt tgaacaacca
     3661 atcccctgct ccacccaacg caccacgtac cccagaatcc cctgcacgtt gttatccagc
     3721 tcttgttgga tctcttgcgc cgaccagtta gcgttttcag ccaccggaat agtcagcaga
     3781 tcgtccagca gcggttcaaa ttcagcattg aactcggtct gggcaatgtt ggcttgcacg
     3841 ctctgtacgt tggtttggtg gtagtgcagc gcatggagcg tagcagcggt tggtgacgga
     3901 acccaggctg tgtttgcccc ggcacgcagt tggtcgccct tctggctgta catgtctgcc
     3961 atcaggtccg gcattgccca catgccttta ccgatttgcg ctttaccgcg cagcccacag
     4021 aacagaccgg aaagcacgtt attacgttcg taggctttga tccacggcgt cgatttcatc
     4081 tggtttttac gcagcatcgg gccagcttcc atcaccgaat gcatttcatc gccggtacgg
     4141 tcgaggaaac cggtattgat gaacgccacg cggttgcgcg cctgagcgat acagctccgc
     4201 aagttcagcg aggtccggcg ttcttcatcc ataatgccca ttttcagggt attcggtacc
     4261 ataccgagca ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac ttcctgcgga
     4321 ccgtgcattt tcggtttcac aatatagacg ctgccagtgc gcgagttttt ctgcactttt
     4381 aaatcataga gggcaatcgc gccagtcatg acgccatcaa gaataccttc cgggatttca
     4441 ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt gcggataaac
     4501 agcaacgagc gtccgtgcag agaaatttca gagccatcgg cggcggtgta ctgacgatcg
     4561 tcattcagtt tacgtacgat ttgccgaccg tttttctcca ttttctcttg cagagtcccc
     4621 tgcatcaggc ccagcagatt gcggtacagc aggattttat cttccgcatc aaccgccgcg
     4681 accgaatctt cgcagtcgag aatggtactg atagcagctt cgacgataac atcgttgatg
     4741 tgcgccggat cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc aatatgcagg
     4801 ccgttatttt tcagcaaaat gcaggtcggc gcagcggcat cgccacggta accgacaaac
     4861 tgtgctggag tacgtaacgt ggtttcttta ccatttttca actggatgcg taattgttta
     4921 tcgaccacct taaacgccac cacatcctga tagctgccgt tttccagcgg tagtgattca
     4981 tcgaggaaac gtcgaaccca ggcgataacc tgctcaccgc gctgcggatc gtagccactg
     5041 accatcgccc cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta cagtgagccc
     5101 cagcgagcgt tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac caccagctgt
     5161 ggccccgcct gactggtgat ttcgctgtca atgcccgtgg tttccaccgt cacgcgctcc
     5221 ggttgcggca ccaggtagcc cagttcacaa aggaaagatt tataggccgc tttatctttt
     5281 accggccccg gattgctgcg atgccactca tcaagcgccg cctgaatgcg atcgcgttct
     5341 gccagcaact gacgattttc tggtgccaga tcgtgaacga tctcatcaac attgtgccag
     5401 aatgcagcag cgtccagttc cacgcccggc aaaacttctc catccacaaa acgtttaaaa
     5461 ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac tcattgtttc tctcctcgtt
     5521 ttcgcttatt tcgccaacac cgctgctgcc gctttcgcga cctgcgcatc ctgtgctccg
     5581 gttaaaccag aaacgcccac ggcaccaata atttgcccat caacaacaac cggtacgccg
     5641 ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt gttcaccatc
     5701 tcttcatagc ccttagtttc acgacgcccc agcgcggcgg tacgcgcttt ctcctgggag
     5761 atgtaagccg caatcggcgc gcaatcgtcc atgcgactta acgccagcag atgaccgccg
     5821 tcatcggcaa cagcaatgga aacagaccag ttatttttct gcgcctcttc ctgacctgcg
     5881 gcaataattg cactcgccat ttgctggcta agaatgactt tagttttcat ttttttattc
     5941 cttttcaagg gcttgttata caatttcaat ccagtgacgc acagaggtac gaccggcgct
     6001 cgccagatgc gtctggcaac caatgttggc ggtgacgatc atttccggtt tgccgctttc
     6061 cagcgcattc attttgttat cccgcagctg gcgtgccaga tcgggatgcg ttaacgcata
     6121 tgttcccgct gaaccgcagc acagatggct gtcgggaacg tccgttaagg taaatccaag
     6181 acgaagtaac acgttttcca cttcgccgtt cagcttttgc gcatgttgta gggtacacgg
     6241 gcagtggaag gccagttttt tatcgccgcg aattgccagt ttttccagcg gttcctcgcg
     6301 cagaagttcg actaaatcga ccgccagttc actgacctga cgcgctttat cggcatataa
     6361 cgcatcgttt ttcagcatct gcccatactc tttgacgaac gcgccgcaac cgctggcggt
     6421 ttgcaaaatt gcctcggcac ctgcttcaat cgcgggccac caggcatcaa tattattgcg
     6481 cgcccgtgcc aaccctttct cctgcgcatt aagatgatag tccaccgcgc cacaacagcc
     6541 tgcttcgtta gctgacatga cgctgatccc cagacgatcc agcactcgcg cagttgccgc
     6601 gttggtgttg ggcgaaagcg taggctgggc gcagccttcc agcattaaaa cccgacgctt
     6661 atggcgcagc ggcggacgcg gtttagcttt caccgtttca gcaggcagtt ttgctctgac
     6721 ctgttccggt aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac ggaagaccgc
     6781 cggacgcggc actacctggc gcaatccttc gcgcagcatt cgctccggca gtgggcgttt
     6841 cactttctgc tcgacaatat cacgcccgat atccagcaaa ttgtgatagc gcacaccaga
     6901 aggacaggtg gtttcacaat tgcggcaagt gaggcagcga tcgagatgct cctgtgtttt
     6961 aagcgtgact tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc cgcgcggccc
     7021 gtccagttca tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa atccgcagtg
     7081 aacacaggcg cgcaggatgc tgtcggcttc cagcgcgcgc gcgttctgcc gcatctcttc
     7141 ggttaatcgg gtttgcatag cctgctcctc aaagttccgc gtacatgcga ccggggttaa
     7201 acacgccgca agggtcgagc tgctgtttaa gctgctggtg atagcggaat aaaggagccg
     7261 atagcggggc aaagccacca tctccggcac taaagcgggt cgcatgaccg ccagcgttgc
     7321 gggcgatgcg atggatttga ttgtcatcgg ctgtcgattt cagccagcgt aacgccccgc
     7381 cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg cgcatcgctg ggtaatgaaa
     7441 tgcgccataa ggtacctggt aacgagaaga acggcagttg ttgttcacgc aattgctgcc
     7501 agaactgacc cgcaacctct tcgccaccca gcagttcacg cgctgctttt accgatcctt
     7561 cgccgccctc aaggcggatc cacaacgcat tgtcgaagta acataagcca ctaatgggta
     7621 atggctggag ttgccactcg gcgatttcac tcatggcttc ttgcaggctg atttcccgac
     7681 gcaggctcag agaggcacgc ggtcgcggta acactttcat tgagatttca gtgagcacgc
     7741 caagacaacc gtagcttccg gccattaacc gtgagagatc gtatccggca acgtttttca
     7801 tcacttcgcc accaaaacgc agatgttttc cagtgccggt aatgatgcgc gtgccgagga
     7861 caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc cgccagcccg caggcgacca
     7921 tcccgcccca ggtggcttct tcaccataat gcggcggctc acagggaagc atttgcccgg
     7981 cgctctccag cgccgcttca atcgccacca gcggcgttcc ggcacgagcg gttatcacca
     8041 gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg cacatccagc gtttgcccgg
     8101 tgactgggcg acctaaaaag gctttgctat tgctgccctg aatcaccagc ggcgttttat
     8161 cgctaattgc ctgattcacc tgctccagca gcgcctgact gtaatcacac tcgcgtagca
     8221 tcaaaagcgc tccagttcag gaaaaggtaa atgaccgtga tgcacatgca tggcaccaaa
     8281 ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg ttcagcaaac catcggggtc
     8341 aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg ctgttgaact gggcgcacat
     8401 ttgattgatt ttttctcgcc cgatgccatg ttccccactg atgctgccgc caacttcaac
     8461 gcagagttcg aggatcttcc cgcccagctc ttccgcgcgg gcaaattcac caggttcgtt
     8521 ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg gcatgaaaga cgttggcaac
     8581 acgtaaatca tattgctgcg ataaacgggc aatgccttcc agtacgccag gcagggcgcg
     8641 acgcgggatg gtgccatcca tgcagtagta atccggggag atacgtccta ccgccgggaa
     8701 cgcatttttg cgaccggccc agaaacgtac gcgctctgct tcgtcctgtg ccaaacggac
     8761 gtcagtcgcg cccgctttca acaagatgta gttaacccgc tcgcagtctt cctgtacgtc
     8821 agactccacg ccgtccagtt cgcataacaa aatcgcttcg gcgtcgacgg gataaccggc
     8881 atgaataaaa tcttccgccg cgcggatcga caggttatcc atcatctcca gcccgccggg
     8941 gataatgcca ttggcgatga tgtcaccaac cgcaagtccg gctttttcta ccgagtcaaa
     9001 gctggctaac agaacccgcg ccacgggcgg cttcggcagc agttttaccg tcacttcggt
     9061 ggtcacgccg agcatacctt ccgatccggt gaacagcgcc agcaggtcaa aaccaggtga
     9121 atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc agcgtttgca cttcaatttt
     9181 cagcaggtta tgtacggtca gaccatattt caggcagtgg acgccaccgg cattttccgc
     9241 cacattgccg ccaattgaac aggcgatttg tgaggaagga tccggcgcgt aatagagatt
     9301 atgcggcgca acggcctggg agatcgccag gttacatacg cccggctgca cgcgcgcgcg
     9361 gcgaccaacg gggttaatgt cgaggctctc tttaaagcgc gccatcacca acaacacacc
     9421 tttttccagc ggcagcgcgc caccagaaag cccggtgcct gcaccacggg tcaccaccgg
     9481 tacacgcagg cgatggcaga cagccagaat cgctgtcacc tgttccattt gcttaggcag
     9541 aacaaccagt aatggacgcg tgcgatacgc gctcaacccg tcacactcgt aaggaatgat
     9601 ctcctcatcg gtatgcagga tctcaagtcc agggacatgc tcacgcagtg ccatcagtac
     9661 cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc tcttcgtaca agatgctcat
     9721 gagtaggctt cgctttgttg tgtggcagct gatttttgcg cgctgcttct gtgaacagtt
     9781 attaagcggg cttttcgttt tcgtctatct ctttagctac cggtcagacc attttttttc
     9841 cagctctgtg accttgtctt ggttaactta atgttaaatt gatgtaacat aatcacttgc
     9901 gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg tcctacctgt gcacgaggtc
     9961 cgggaatgaa agatgaacgt cgccctattt gcgaagtggt tgcagagagt atcgaacggt
    10021 taattatcga cggcgtactg aaggtcggtc agccgcttcc ctcggaacgt cgactgtgtg
    10081 aaaagctcgg cttctcacgc tccgcactgc gtgaagggct gaccgtgctg cgcgggcgcg
    10141 ggattattga aacggcgcag ggtcgcgatt ctcatgtcgc acggcttaat cgggtgcagg
    10201 acaccagccc gctgatccat ctgttcagta cgcagccgcg aacgctgtac gatctgctcg
    10261 acgttcgcgc attactggag ggcgaatcgg caaggctggc ggcaacgctg ggaacgcagg
    10321 ctgattttgt tgtgataacc cgctgttatg aaaaaatgct cgccgccagt gagaaccaca
    10381 aagagatttc gctgatcgaa catgcgcagt tggatcacgc tttccatctc gccatttgtc
    10441 aggcttctca caatcaggtg ctggtgttta cgctgcaatc attgaccgat ctgatgttta
    10501 attcagtgtt tgccagcgta aataatctct accatcgacc acagcaaaaa aagcagatcg
    10561 atcgccagca tgcgcggatc tacaacgcgg tgttgcagcg gctgccgcac gtcgcgcagc
    10621 gcgcagcgcg cagcgcgcag cgcgcgatca tgtacggacc gtgaaaaaga atctccacga
    10681 tatcgagctg gaaggccacc atttgattcg ctcggcggtg ccgctggaga tgaacaaggt
    10741 gggaatgtga gcagagtttt accctatccc caaggcgagg ggaccgcccc tgcacttaac
    10801 aggatctggt gttaaatctg ttcatctata ccctctcccg aatgaccgcc cctgcgctaa
    10861 acaggatctg gtattaaatt tgcttttcat acaatcggta acgcttgtac ggctccgccc
    10921 caatgcgttc cagcatgtta ttcatgcctg tattggtttc gaggatccat tacatctcca
    10981 gcgcatcgat cttccggcgg gcaaacggat cgcgtaaggc ttcaatcaat aacagcgcaa
    11041 tcaccgggcc gatgcggctg aactgatact cgtcgcgcac gcccatcagc ggtactcgcg
    11101 cagttcgcac accgctgact ttcaaacgcc acagcaattt tgcccagccg aagggaaaga
    11161 gcgatccgtt cagatcggca atcgcctcgt tgatgttagg caagccgaca ataaacgcgc
    11221 agggtgcaga atcaatctca gcgatgtaga tcatatcgtc cggcaccaga tatttaagtt
    11281 ggtcgcccat ggtcgcgaat tcatgttcgg taaacggcac aaatccccag ttgtgctgcc
    11341 agccagagtt gaaaatctca cgcaggatct gcatctcttc ggcaaaccgc tgacgattga
    11401 tgcagcgaat ggtcaccttt ttgcgcacct gatccatccg tttttttagc gccggagaga
    11461 aagtgagatc ggttcgctgc atccaccacg ccagtaaatc aatgcctttg tgataaccca
    11521 gttgttcaat atgcgcggca taccacggtt tgccgtgtgg catcatcgca cagggtggtg
    11581 tgtcaaaacc ttcaatcagt aatccgcttt cctgattgat attcaggctg aaaggcccgc
    11641 agatcttact tgcaccttgt gacttcaacc acgcttccgc tgcgccaaac aacgcggcaa
    11701 aatcctgcgg atcatcaatg gcgtcaatca tgccgaaatg accggtatct tcaccgtaac
    11761 gctcacggtg caaggtatct atttgcgcgg taatacgccc aactatctgc cccgcttttt
    11821 ttgccaccca cgcctgccag atgatatggt ccgtccccgg atttttcgca gacaaatgct
    11881 cgttgcgttc aatgaataaa gggggtatcc agtttggatc gtcgggacaa agtgatgacg
    11941 gaaaagcgat aaatgcctta aggtcatttt tattaattac tttttcaatt ttaatcatca
    12001 gcagatcgca ttccatatct ctgtggataa caaataatta atggtgaaaa acaggttgca
    12061 acagcaacga gcggcatctt aataatcaac tcaatgaata caaacgacga ataaatagta
    12121 ttaattaatg attatgataa ggtcagaggc tacacagaaa cgcctcaatg ccttttgtcg
    12181 atttaaggcg accggacaag gcttcttctt aatatattca taaattaaat atcattcata
    12241 ataaaaataa aaaactcaaa ctcattattt aaatcaatgt tcattttcac accgatgaaa
    12301 atactatctc atttatcttt tatgcctata taatccaccg cattattgtc attttatttt
    12361 tgatatccaa ttttttcgtg gtgtatatgt ctaataaaat ctttacgcat tccctaccta
    12421 tgcgctatgc cgattttcca acgctggttg atgctttgga ctacgccgct ctgagtagag
    12481 ccggaatgaa tttttatgac agacgttgcc aacttgaaga tcaactggaa tatcagacat
    12541 taaaaacgcg tgccgaagct ggtgcgaagc ggttgttatc gctgaacctg aaaaaaggcg
    12601 aacgcgtggc gctgattgcc gaaacgagta gcgggtttgt agaggctttt tttgcctgtc
    12661 agtatgctgg cttagtcgcc ttcccgttgg cgattccaat gggcgtcggt cagcgggatt
    12721 cctggagcgc caaactccag ggtttactgg caagttgcca gcccgcagcc attatcactg
    12781 gtgatgagta gttgccactg gtcaatgccg cgacgcataa caaccccgaa ttacatgttt
    12841 taagccacgc ttggtttaag gcattaccgg aagccgatgt tgcgctccag cgtccagttc
    12901 cgaacgatat cgcctacctc cagtacacct ccggcagcac ccgttttccc cgtggcgtca
    12961 ttatcaccca tcgcgaagtg atggctaatc tacgtgctat aagccacgat ggcattaaat
    13021 tacgccctag cgaccgctgc gtctcctggc agcctttcta ccatgatatg ggactggtcg
    13081 gctttctcct gccccccgtc gccacgcaac tttcagtaga ttatttgcgc actcaggatt
    13141 ttgccatgcg tcctttgcaa tggcttaaat tgatcagtaa aaatcgcggc accgtttccg
    13201 ttgcgccgcc gtttggctat gaattgtgcc aacgccgcgt gaatgaaaaa gatctcgctg
    13261 aactggatct ttcctactgg cgcgtcgctg gtattggtgc tgagccgatc tccgcagaac
    13321 aactccatca attcgctgaa tgtttccgtc aggttaactt tgacaataaa actttcatgc
    13381 cgtgctacgg actggcagaa aatgcgctgg ctgtcagctt ctctgatgaa gcctccgggg
    13441 ttgtggttaa cgaagtggat cgcgacatcc tcgaatatca gggtaaagcc gtcgcgccgg
    13501 gtgcagagac acgcgccgta tcgactttcg tcaactgcgg caaagcgttg ccggaacatg
    13561 gtattgaaat ccgcaatgaa gcaggtatgc cggtcgcgga acgtgtggta ggccatattt
    13621 gcatctccgg tcccagtctg atgagcggtt actttggcga ccaggtttcg caagacgaga
    13681 ttgccgcgac gggctggtta gacaccggcg acctcggtta tctgctggac ggttatctgt
    13741 atgtcaccgg acgcattaaa gatctgatta ttattcgtgg ccgtaatatc tggccgcagg
    13801 atattgaata tatagcggaa caggaaccgg aaattcattc tggcgatgcg attgcttttg
    13861 ttaccgccca ggaaaaaatc attttgcaga tccagtgtcg gatcagcgac gaagaacgtc
    13921 gcgggcagct tatccacgcg ctggcggcac ggatccaaag cgaatttggc gtgaccgcgg
    13981 ctatcgatct gttgccgccc cacagtattc cccgaacgtc ctccggcaag cctgcccgtg
    14041 cggaagcgaa aaaacgttat cagaaggctt atgctgccag tcttaatgtg caggaatccc
    14101 tggcatgaat caaactgtcg cggtgacggg cgctaccggg tttatcggta aatatattat
    14161 cgataacctg ctcgcccgcg gctttcacgt tcgcgcattg acgcgtactg cccgcgctca
    14221 cgtcaatgat aatctcacct gggtgcgcgg ttcgctggaa gatactcatt cacttagcga
    14281 gctggttgcc ggagccagcg tagtggtcca ttgcgccggg caagtgcgcg ggcacaaaga
    14341 agagattttc acccgctgta acgttgacgg cagcctgcgc ctgatgcaag cagcaaaaga
    14401 gagcggcttt tgccaacgtt ttctgtttat ctcttcgctg gcggcgcgcc atcccgagct
    14461 ctcctggtat gcaaaatcca aatacgtcgc cgagcaacga ctggcagcaa tggcggacga
    14521 aattacgctg ggcgtttttc gcccgacagc cgtgtatggt cccggcgata aagagttaaa
    14581 accgctgttt gactggatgc tacgcggcct gctgccacga cttggtgcgc cagatacaca
    14641 actctctttt cttcacgtca ccgattttgc gcaagcagtg ggtcagtggt taagcgccga
    14701 aactgtacag acgcaaacct atgaattatg cgatggcgtc gccggtggct atgactggca
    14761 acgcgtacga cagcttgccg ccaaagcccg ttgtggttcc gtgcgaatgg ttgacattcc
    14821 tctgccggta ctcacaggcc ttgcggatat cagtaccgcg ttgagtcgcc tggcaggtaa
    14881 agaacctatg ctgacccgct cgaaaattcg tgaattaacc cacgccgact ggtcggcaag
    14941 taataaccgt atttctgaag atatcaattg gtttcccggg attagcctgg aacacgcatt
    15001 acgcaacggg ctattttgat tgtcttgttt taaaggtgtg ttaacgtttc tatggtaaat
    15061 cgtgaaatag taatggatta catcctttcc tgtttacagg atttggtcga aaacggtgtg
    15121 gaaattaagc ccgacagcga tctcgtcaac gatcttggcc tggagtcgat caaagtgatg
    15181 gatctcttaa tgatgctgga agacagattc gatatttcca ttcctattaa tattctgctg
    15241 aatgtcaaac cccctgcgca attaatggaa actctacttc catggctgga gaataaataa
    15301 tggggctata cgataaatat gcgcgccttg ctggcgagcg tctgcaattt agtgacaacg
    15361 ggttaacacc gtttggcacc tgtattgatg aagtgtattc cgccaccgaa ggccggatcg
    15421 gcaataaaaa agtgattctg gccgggacca ataattatct cggtttaacc tttaatcatg
    15481 atgccatcag cgaaggtcag gccgcgttag cggcccaagg caccggcacc accggttctc
    15541 gtatggcaaa cggcagttat gcgccgcacc tggcgttaga gaaagagatt gccgagtttt
    15601 tcaatcgccc taccgctatc gttttttcaa ccggttatac cgctaattta ggcgtcatca
    15661 gcgcacttgc cgaccataac gccgtggtgt tactggatgc cgacagccac gccagtattt
    15721 atgacgcctg ttctctcggt ggcgcggaaa ttattcgttt ccgccataac gatgctaaag
    15781 atctggaacg gcgcatggtt cgcctcggtg aacgggcgaa agaggcgatt atcatcgttg
    15841 agggcatcta cagcatgctc ggtgacgttg cgccgctggc ggaaattgtt gatatcaaac
    15901 gtcgtctcgg tggttatttg attgtcgatg aagcgcattc gtttgtcgtg ttaggcgcaa
    15961 cggggcgcgg actggctgaa gctgtcggcg ttgaagatga tgtggatatt atcgttggta
    16021 ctttcagtaa aagtctggcc tcgattggcg gttttgctgt cggttcggaa gcgatggaag
    16081 tgctgcgcta cggcagccgc ccgtatattt ttaccgcctc tccttctccg tcctgcattg
    16141 cgaccgtgcg ctcgtcgtta agaactattg ccacacaacc agagctgcgg caaaagctga
    16201 tggataacgc caaccatctg tatgacggtc tgcaaaaact gggttatgag ttaagttcac
    16261 atattagccc ggtagtgcca gtaataatcg gttcgaaaga ggaaggttta cgcatctggc
    16321 gcgagcttat ctcgccgggc gtgtatgtga atcttatcct gccgcctgcc gcgcctgcag
    16381 gcatcacgct gctgcgctgt agcgtaaatg ctgcccacag tcgtgagcaa atcgatgcca
    16441 tcattcaggc atttgctact ctgaagcagt aatgtgagcc cgccgtggtg tttgcgccac
    16501 ggcgggttta atgttgtctg tcataccaat acaccaggct caacgccacc aacacaaacg
    16561 ctaccggtaa accgagcgcg gtaacaggtg cgcttaacgc aaatagcaat cccagattaa
    16621 ccatgatttg atagctgatc caggtgagta agcccacgat gacgcctaca gcgagacggc
    16681 tacccatccc aggcgaacgc ggggcactaa aggtaaacgg cacagccagc aaaatcatcg
    16741 ccagggtcag gatgggttgc cccagttttt cccacagagc aaggcggtat tcgctgctgg
    16801 gttgcccggt ttcctgcaaa taatggatgt aatgattaag ctgacggaca gaaaaagtat
    16861 tccccggcat tgataactct tcaagattca tactggtgaa ggccgattgc caggcaagat
    16921 tatctaatgt ttccagcgtt tctttgccat tcagccattt tttatgattc acaccatgca
    16981 ggacccacgt tttgtcgtct tcaatggtgg cactgcgtgc ataaatgtag gattcaagcg
    17041 agagatcgtc gcgataatga aatatctcca cgcccaccgg ctggccctgc tcgttcagcg
    17101 atttcactgt cacaaattca ttaccacgcc tggcccacag catattgccg gtaatgtcat
    17161 cgtcctcccc caacgcggtg gcggtcgatt tgatttgcaa cgcctgttgc tgcaatggcg
    17221 acgccaccca ctcatctatc gcgcctaacg aaacagtcca caatatcccg gcaaccagcg
    17281 ccaccagtgc aatacggaag atagaaaacc ccgtgctgcg aatggcggta agctcactgt
    17341 ttttcgataa ctgccccagt ccaacaatac cacccaataa cgcgataaac ggcgaaagtt
    17401 cgaccagtgt gcgcggtaag gtcattagca ccaccagcac cgcctgagtc cagcgataac
    17461 cgcccgggct gacaccatcc agttcgttaa tcaggttgaa ggtggtaaaa agcggcagca
    17521 atagccctgc agcggcggca aaaccgagaa agagatgacg gattaaatag cgactgaaaa
    17581 cattcatcgg gaaaatttct gcagcaaaga gaagtcacgt gcgaccagta tcagcaggcc
    17641 cattagcatg agtccgggca ctaaccacaa accaggaatg aggggaatcg cgccgttagc
    17701 caccagtgta cggcagacgt cgccgccata aaaaatggca ataaacagca acgtcagtgg
    17761 caataacgtt gaaaatcgcc cttgccgcgg ttttacccga cttaatgaaa tggccagcag
    17821 cgccatcaac aatgcggtca ggccacggct ttgtcgccat tgcagttcgg catgatcggc
    17881 aggaaaggcg gagcgcgcca gctccgtgac cgatttcgct ttgcgtttga cgttcgggct
    17941 ttgatccagc ggattcagat gtaattgcag attacggtag atctgttcgt tgtcgtcacg
    18001 cccctgatga tcgagaagat aggcagtgcc gttgtgcaac ataacggtcg gtttctcggg
    18061 tgatgggtca accacatcaa ccgaacgggc gcggaaaata cgggttcgat tggcagtaga
    18121 agtataaatc agcgcgtcag tcaggcgatt attatcctga tcaaccgtct gcgaaaggat
    18181 cattcgtccg ttatcgttag tgttaaattt ctttgcccgc aactggcgaa catccagctc
    18241 cgactgcgac tgctgctcca gttgataaat ctgcgcataa gcccacggac gaccatacat
    18301 cgacagaagg gtgacaaaaa tacttagcgg tatcgccaga taaagaacgg ctttgtacaa
    18361 gcgtcccgga ctgccccccg ccgcagagat agcggtaatt tccgagtcgg tatacatctg
    18421 ccctagcgtc acgccaactg acacatacag accaacaggt aacaacatct ccagtgcaat
    18481 cagtactttg tagaaaacga tatccagcac aacatcaaga gctaatgtcc catttgccgc
    18541 ttcggtcaga taacgctgtg cggagtagct ggcgaaaata aagatcagaa aaccgacgat
    18601 aatcagcacc agacggcgcg taccacgcat gatgtagtgt tcaaccagct tcatctcact
    18661 aacgccctca atcctttgag ttttttattc attgatcagt ttctgcgcca acgcaatgac
    18721 atgatctacc tcttccggcg tcaggctgcc gtcctttgcc cactgaattt gcccggcttt
    18781 attcagcacc agaatggttg aactctgctc cggcaagcgc caggcaacgc gtccctgtcc
    18841 attgccgtca ataacgaatt gcgcccacgg atagcggcgt ttattttttt cgattttgcc
    18901 aacaacaaaa tagccagtac caaagatggc atcatcggtg ttcacaatgg tggtgggctg
    18961 aaaacgatcc tgcggaaagt ttgccgcttc aaccgcttta attaacagtg agttcttttt
    19021 cttcgccgac ttacgcccgg cgatatactg aataatgcgg acttttcccg ctaattgtgt
    19081 gctgttccac ttttggtagt gaatttcgtc gtcgctattc agaagcagct cgccgccttc
    19141 ctggatataa acaggggtaa ccgttttgcc agtgacgaaa ttgtgtgcct gcacacccgg
    19201 ggctaagagc atgataatca acgcaataat caaacgagat gacatcattg ctccggctat
    19261 agcggaaaac gcgaaatcag cagctctaag cgctgacaaa tatcgctatc cagcgccaac
    19321 ggtgcataat acgactggcg gttttccata tcatccgcca ccatgtcgtc gtgtttatct
    19381 cccatcaggc cattaatggc ggcgaggaaa cgggaaatct gtaacgcctc tttattttca
    19441 ggatcaagcg atcttaaggt gtcgtaataa gaaacctgct ggctgatgat ttttcgcaca
    19501 tcatgaaatt ccctgccggt tagtcgcggt ttggcaataa tctctttcag ggtatgcatt
    19561 tgcttttgcc gataggcaat aaaactgctt acgctttcta actggctatg gcgtaatgaa
    19621 tagttcacca acgaccagac cggagagctt aaataaatcc gtaacaggtt gccgtaatac
    19681 gacacaatat ttttcttacc gttacgaaag ccgtcctgaa aacgccccag aaaaacggtc
    19741 gttttgccga ataaaaatcc ggcctgatgc ttctttaaat acagccgttg cgcaaaacgc
    19801 aggtgcttgt agcgcgctcg catgtattta aattgtttgc gctcatcagc agtcagctca
    19861 tcgcctttgg cttgtttgcg aactaaacgc agtaattcct gacgatcaac gccattgagc
    19921 cagaattgca ggcataacgc gtaattatca tgaatgattt cggtggtaca acttagtgca
    19981 atagtcggcg gcaaactaac ctgtgcatca acgacatcat cttcttcaat cgcaacaaat
    20041 agacgctcgc tcgcctcaag cgggaaatat tttctcatca tcaggatttc tctccggctg
    20101 cgttataaag caaggaatgg cgctgaaaaa atgcctttaa ggttggaatt aacgagagcg
    20161 ccacgtccag acattgccca ccgaagtaag caaacgctgc tcccagcaca ccaaacagat
    20221 gacagaacat gaacagcaat acgatgtagc cgattgaggc gatggtttgc gccacgagaa
    20281 aagcgcgctg ttttcccgcc atcaatagca atgactcctg cgggaagccc agcatcgaaa
    20341 tcacaattgc gcccagcatc acctgaatca ggtcatacgc ttcgagatac ttaacgccaa
    20401 acaccagcga aatgagcggc ttgccgacga tcaacaccgc gagcgccacc agaataccga
    20461 ttccgcccgc cagtaaaccg gacttcacac ctaacaacca cggtctggtg gtgcgcggat
    20521 ctaaacgcat cacctctggg taaaaacttt tacccagcaa acccgctggc gttccggcgg
    20581 cgtcgaagaa tgtcatggcg attttaaata acccagcggc agcgggtcct aacacgatcc
    20641 ccaccaacac tgtgctgcac gagttacgcg ccgaccagat ggagtgggca atgttggttg
    20701 accagacaaa actccacgcg cctttaatgt gtcgggcaga ctcaaacaga ttcaatttga
    20761 aggcgttatg gatatttcta cggcgtaatt cgcgcgcggc aaaccaccag tacatggtgc
    20821 cgccaaccag attcgacacg taccaggcaa taacaaaacc cgcaaaacca aagtcaaaat
    20881 accaggctgc gacgctcccc gctgcgcgca gaaaaggttt cgtcgcctgc tgtacagcaa
    20941 tcaaatcaaa gcgatctacc gcacgcagaa tgccggtcgg cgtggaggaa gccattgaag
    21001 gaatgagcgt gcaatacagc gccgccagcc aaaagctttg gtcatccaga cctaatgaat
    21061 gggaaaggaa tggcagtaag gcaatgccgc cgacaatcgc caccgcgccg ctgacgatat
    21121 ccagcgagaa ggaaaatgag acgatattgc gaaattgctg cggattattg ttggtcaagg
    21181 ctggcgttcc gtactgaacc accagctgcc atgtctgaaa cttaataaaa tcgctgatcg
    21241 acttggcgta cgattgcaca atcaccagta caccaaacat ggcgggcgtc atccctttac
    21301 cggcacacga gagcgccaac agccccagca aggcgctctc gacattactg gagcctaacc
    21361 aggcgctatt gcgaataatg gtgcgaaacg cgccatctgc aaaccaatgt ttgatgttaa
    21421 aacccgccaa ttcagcctga ccttatcgtt gatagtaaaa aatatcccgc cagccttaag
    21481 ttaaacttcg gcggtcagaa acgatggcaa ccagagaaac cgccttctgt acctgttcca
    21541 gcacttcgct gtagggcgct ctggaatcaa tctcaagaat ttttgtgccg ttatagccaa
    21601 tcgtcgacat gacgccgatt ttgtcctgca gctcggcata ctcatggtca ggcttgcggg
    21661 agatggcagt atcaatatca atccccaaac gaatgattaa ttcaggacgg tattgcgcca
    21721 ttttttggta taaactccgt tcgcgctgcg ccagaaacat gctgattttc ccggtcgcac
    21781 gttcgacgcc gatccccggt ccgtcataat aaaagcccga aatttcagcc tgcgggaagc
    21841 gatcgctgac caccagaacg ccactttgcg ccagtcgctg aaccttgcgt agattcgcca
    21901 ttcttcgcag cgagaagcag tacataatca ccgccgccca tagcgccgga gatttggttt
    21961 tcatgctttg ggttttcgag gatttggccg ccagtcgccg ttccagccag acgccaacca
    22021 acggcaatcg tttgattttg tcgccgtctt cgccggagag cagccccaga tagcggcgct
    22081 cggtttgcca gtgttgttgc agcgatttca ccaggtcggt ggtcagcgtg gatttaccgg
    22141 tgccatcaca cccgaccacc gcaatcagcc ccggaatgta gttgggctgc ggcgcggttg
    22201 tactattaac agtttgtgtt tgtagtgcat ccattcgtta aatcatcccc tatccatttt
    22261 ggtgatcagg aaagcgcggc gtgaattgcg cgtagtgacg cttgcataat ttcatcggct
    22321 ggttgccgac catccagttc aaggatcttg gcgccattga atgtcagttg cggcgtaacg
    22381 gcgatttttt cctgtaacgc tgccagttgg tggtcaggtt tacgcgcaaa cgcggtttgt
    22441 tcatcaatgc caagacgaat caacaatacg ggtaaataag acgccatcca ttggtacagc
    22501 ttcaactcgc gctgccttaa catttttatc caaccgttac cgcccgtggt ttttgccaat
    22561 tgcgggccat caaagcgaaa acccggcact tcaacctgcg ggtagcggtc ggtgatgagc
    22621 agatagcctt gctggctttt acacaacatt ttgcgaaact tgtacgcccg ccagcaggaa
    22681 agcagataga taaccagcgc agtaatattg ccaggcggtg ttgagggctt ttcgtgtaca
    22741 tgtgccgctt tacttcgcag ataacaccca caaggtgcgc caataacagg gagctgtgaa
    22801 atccattcgc caattcgccc ggacgattgc ccgagataaa tgtgttctgt tggcattctt
    22861 gctgccagtt catttaccag gcttgccgtg agggtcgatt tacctgaacc atcacatcct
    22921 acaatggcaa taacacgcac tggcgtggaa ttaattattg acatattacg ttgattcacg
    22981 aaaaacccgg cagtaaataa tgtattgaat attacgtggt ccgtgctcag aatatccgtt
    23041 caaccttatt gtgacaatgg ttaattgtga cattgacctg aattacgctt tcataaaaac
    23101 atattaacca aataaatatt tttaatggat atttaaatta aaggatatat tcatgcagtc
    23161 aataacacct ccattaattg ccgttattgg tagcgatggt tcaggcaagt caacggtgtg
    23221 tgaacatctt attaccgttg tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa
    23281 acaggccgga aatgtcggtc gtgcagtgac aaaattacct ttgatgggaa aatccttaca
    23341 taaaacaatt gaacgaaatc aggtgaaaac agcaaaaaaa ttgcccggac cagttccggc
    23401 gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg
    23461 tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa ttcctggcgc
    23521 ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct catggctggc
    23581 aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc tggtcatcaa
    23641 actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac gggaatcgct
    23701 ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac tggttgatat
    23761 cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga ttacggattt
    23821 tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc aggaccgggc
    23881 attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca gaaagaaaaa
    23941 ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt ttacgctgta
    24001 acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg tgtgttgtgg
    24061 agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc atttgtgccg
    24121 ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta
    24181 cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc
    24241 acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct
    24301 ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca gagatgcaca
    24361 gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat
    24421 tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt
    24481 gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt tccatcgcaa
    24541 tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtaacggcat
    24601 cgcgggtacg ggtaatttca tagccggacg cattcatatt gacgacgaag ccagcagggg
    24661 caatccccac cagtaccagc attaccaggc cgatcccttt ctgcccgtcg ttcacgccgt
    24721 gcgaaaacgc cacgcctgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt
    24781 tacgtttgcc ctttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt
    24841 tcgtcccact ccagaaacgg cgcagcagga aaatcagacc acccgcaatg accaggccga
    24901 cgatagggga aacaatcagc gaggagaaaa ttttggttac ttcacgcagg tttaacgcat
    24961 ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa
    25021 tcaaggtgtg cgaactggag gccggtaaac caaagaacca cgttcccagg ttccagataa
    25081 tcgccgccag cagcatggaa aagaccatcg ccagaccgtg agttgacccc atattcagca
    25141 acaaatcggt tggtaacata tggacaatgg cataggcaac gctaagtccg cccaataaca
    25201 cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggctgc atggcacgag
    25261 tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga
    25321 acaacacaaa tgccagagca agtaataaca aaagccccgt atatatatca aggccaacaa
    25381 ataaatttag catatattag attacgccat tttgaattta cgaacggacg cattatcagt
    25441 gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag agtgttattt
    25501 cttgtgtatt agacaataag cattcattca aaacctttag aaataatcaa aacacaggta
    25561 ttccactggt taatatattc acatatgaaa tgaataaata ttggaatata taaatattga
    25621 atattttgat taatccccgt actaattatt cttcataatc agtacggggt tgctacaaca
    25681 tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg
    25741 attcatcacc gcgcaaacac gtcccaccgt agttgccgcc aacggtgaag gtacatacct
    25801 gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct
    25861 cggcaaattt accgctggtt ttgtccagca cctcttcatg atggccgacc aggtcgatat
    25921 tgctaccaca gcgaccggcg atcggtttca ctgcgtaacc tgttttcacc aattcatcat
    25981 taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg
    26041 gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagacc agcacttccg
    26101 ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca
    26161 ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc
    26221 aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc ccggcagcat
    26281 cccagcccag ttcatccagc ccgcgcaaga tacgcgtttc aaagcccgcc tggtgcagcg
    26341 cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga
    26401 caaacggacg tgcacgactg tgtttccagg caccagccag ttcgttaatc agcccttccg
    26461 ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg
    26521 cttcggtatg acaggaggcg gagtcggcgt tgtattcgta aaccttcagg ccacgctcat
    26581 ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac
    26641 gcaaacgtgg ccagaggatt ttcggaatgt cgaacagcgc cagcaggttg tcatctttca
    26701 gcaccttgtc ggttgcgtga agatacatca gatgcagctc gttggtggct ttaattagct
    26761 cctgctcggc actctcggta atggtgtagt aatgatacgg atcctgattg atcacctgac
    26821 cgttggcctg cacataggcg ttttgtagcg gatctttttc atccagccat ttaccgtcaa
    26881 actggccttt gttttccaga cgcgctccac tgattttcag cagctcgcct gcaatttccg
    26941 gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt
    27001 catcaaaagt gtctttaagg gtatagcagc cgttttcgac caccatctcc agctcgcgcg
    27061 tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt
    27121 tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt
    27181 tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct
    27241 gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc
    27301 acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa
    27361 attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg
    27421 cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc
    27481 cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc
    27541 tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt
    27601 acctgctcag cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg
    27661 ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc
    27721 aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt
    27781 ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cgttttctgg
    27841 tccgacgcat gaaaaaacgc tgcccgttgg caaacatcca ttgcaacttt attcgctggg
    27901 aacgccgaac ggtcagaaag taacgattat gcttgaggag ctactggcgc tgggcgttac
    27961 tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg
    28021 ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc
    28081 gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga aatttggcta
    28141 cttcctgccg caggatctgg caaaacggac tgaaacgttg aactggctgt tctggttaca
    28201 gggtgccgcg ccgttcctcg gcggtggttt tggtcacttt taccattacg cgccggtgaa
    28261 aattgagtac gccatcaacc gctttaccat ggaagccaag cgcctgctcg acgtgctgga
    28321 taagcaactg gcgcagcata agtttgttgc gggcaatgag tacaccattg cggatatggc
    28381 gatttggccg tggtttggca acgtggtgct cggcggcgtg tatgatgccg cagagtttct
    28441 ggatgcggga agttataaac atgtgcaacg ctgggcgaaa gaagtgggcg aacgtccggc
    28501 agtgaaacgt gggcgtattg ttaaccgcac caacgggccg ctgaacgagc agttgcatga
    28561 gcgccatgac gccagtgatt ttgagacaaa tacagaagat aagcgtcagg ggtaagggtt
    28621 ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc
    28681 gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacaaaag ctaatcatca
    28741 cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc
    28801 ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc ccagcagatc
    28861 ggcagggtta ccttcgcaaa tcagggcgat attcacgtcg cgcttgatac cacatacttc
    28921 aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat
    28981 acacattgtt aactccggtt atttcgattg ccggacaaac gcgaaaaaga acctttagcg
    29041 ggtgttccct ctcaccctaa ccctctcccc ggaggggcga ggggaccgtc cgtgcgcttt
    29101 ttaggcgttg tcctcagtct tattcaactt caatactttt gacgatcagc gaatcgccgg
    29161 tatcgacacg caaccgctcg ccgtgacaga gcggacactg cgcatcgtgc tgatgaatct
    29221 ccaccacctg gctgcaatcc cagcaccagg cctgggcggg tttatagacg atatgtaaat
    29281 cgcacccttg cgccaccgtt ccgtggcaga caatttcaaa actaaaacgg acggcgctct
    29341 cctcaacgca ggagagcgcg ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt
    29401 gctgctccgc ctgccgttgg ataatttcaa cggcgctctg gcaaagcgac aactcatgca
    29461 ttttcgcccc tcctgcggcc aaacagcagg gcgcgacgcc ctgcgtgtgg tacatccgga
    29521 ttggtgactg gcaacgaaag gatcattcgt gcgcagtcat ccgtcaggcg ttggccttct
    29581 tcaatcgaca tgctgtgcga aagcggcgac atcagcgaac aggagaggta ttgcgaaaca
    29641 ccgtccagtt cgccaacggt aaaggtcatg gtgccatacg gcagttgcag accaattttt
    29701 tcactcactt tgcgcagcgg ccagagttga tccgggccgg ggaagataac tgcactcagc
    29761 atccacgggg tgatcacaca ccccgtccac tgaccttcga acagcgtaaa atcagaaaca
    29821 tacaccggca ttgaaggatg cagaaaagag agatcgtgca tcgaacgccg ggcaatttct
    29881 tcaaacgctg cctgtacttg cgccttcggg gaggtctgga aacctgctat ctcttcagtc
    29941 atgaatcgcc tcccgtggga tggcttccac gcctgattcg cgcagcgcag ccagaaccag
    30001 ctcaagcgca ggttcaatca ttgcttcaac cgtcggcgtt aagccgatgt gtggctccag
    30061 cgattccggg atcacgccga ccagggtcag ctttttcgga aactcaccgg tgaagcgcag
    30121 ggccgacaat acgtcggcca ggccaagctg atgcggagag attttgttgg taaacaacgc
    30181 cggaacttct tcatcccgca ggatcatcat cgttcccggc gtgttctttt tcgacacaat
    30241 ggcatccgca ataatcagat gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc
    30301 cgtgccgcca tcgaggatct caacataatc cggcagaatg tatcgttgct ctaacgcttc
    30361 gacaatccgc acgccgatgg cttcatcggt cagcaaaata ttgccgaccc ctaagactaa
    30421 aatacgcatt acagaacctt cactgaaacc acttcgttgc cgtcggcatc cactacgtgt
    30481 accgcacagg ccatgcacgg gtcgaaggag tgaatggtac gcaccacttc cagcggttta
    30541 ttcggatcgg caaccggtgt acccaccagc gactgctcgt aaggaccgac gtcatcattg
    30601 aagttacgcg gaccagagtt ccaggttgat ggaacaaccg cctggtagtt gctgatgata
    30661 ccgtctttaa taaccatcca gtgagagagc ataccgcgcg gcgcttcgag gaagccaaca
    30721 cctttgaact cacccgttgc cggaatgttc ggcttcacaa aggtggtgtg atcgcctttg
    30781 ccgatattgg tgatcagtgc actgtattgg ttttgcagga tatcctgcaa ttcacagcag
    30841 tgaacggtac gaccaataat acggcccagc gtggagtgca gctgtgccac ttccagcgtg
    30901 ttgccagtca gtttctgata aatcgcaacg atttcattca gtttgttctg ggtagattcg
    30961 cgacctgccg ccagtttcac cagcatatta gccagcggac ccacttctac cgttttgccg
    31021 tagaaagtcg gtgatttcac ccaggaatat ttcccgtcgt cagaccaacc atcataagcc
    31081 ggaatggtgg taccttccca tggcgcctgt ggcgcttcgt ctttatacca ggagtgcttc
    31141 gcgctttcct gaatgccttt gatcaggtat tcatcggaat gagaagtgat cggacgatac
    31201 gaggacagat ccgcattctc aatgtagccg cccgggaaca ggaagctgcc gtttttgctg
    31261 tcggtcggga attccggcac gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat
    31321 tccgggtaga acgcggcgat aaccgcggta tcaaccttat aaacctgctc aacaaagtcg
    31381 ctcagtttgt cgatgaaaga cttgatgtac atcaggcgct caaggttcaa cacgcccaac
    31441 ccgtcgaggt tgattgggtt cgcgacacca ctcactgcca ggttctgaat gtgcggcgtt
    31501 ttaccgccca gcagcgccac gacgcggtta gcgtcacgct ggcactccag cgcctgcagg
    31561 tagtgcgcta ccgcaatcag gttcacttcc ggcggcagtt tcatcgccgg gtgaccccag
    31621 tagccattgg cgaaaatacc caactgaccg ctggcaacca gatctttgat cttgttctga
    31681 actttagtga actcttccgg actgttcaga tgccaggtcg aaacgccttt cagcatttcg
    31741 gaggctttgg ttgggtcagc ttgcagtgca gaagtgatgt ccacccagtc cagcgccgaa
    31801 agctgataga aatgaacgat atggtcatgc gtggtgtgcg cagccagaat gatgttacgg
    31861 atgtattgcg cgttaaccgg aacgtcgata ttcagcgcac tttctgccgc acgaacggaa
    31921 gacagcgcgt gagtggtagt acatacgcca cagatacgtt gcacaatcat ccatgcatcg
    31981 cgcggatcgc ggtttttcac gatctcttcc atgccgcgcc acatggtacc ggaagcccat
    32041 gctttcgaaa cgacgccatt ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg
    32101 gttaccggat caatagtaat tctctggctc atgctttgct cgcctcatga cgattatgat
    32161 cgttttgttt taaaggagga agtatcggca gtagacgaat gagtacgatg taagcgcaaa
    32221 tctcaatagc cacaaaacca atagaaatca acagttcttc ccaggtcggg aagtaggcgt
    32281 aaccgccgcc cgggttgaat gccaccaacg aataggtcag acgccaggtt gcacaaccta
    32341 acagtgcgct cagtgctgac aggaacagca tgcgggaatc attacgcagc ttcgccacac
    32401 gcagaacgac cagcgggaag agcatcagca ggacttcaat ccagaacatc acggagtaga
    32461 agtcaccggc aaacgctaac gacagcttgt cgcgatagat aagctcgcca aagcgcagcc
    32521 cgatgaaaat cgccagcaac acactgatgg tgttggtcag cttgacgaac aggctctttt
    32581 catccggacc gttgccacgc agacccgcct gcaccagtga accttcaaag atgacaatcg
    32641 agaagcccat gatgaacgcc gtcagcagcg agaacagcgg caacatttca tagctctgcc
    32701 agagcggatg caccttgtag cccgccgaga tcatcagcga ccccattgaa gactggtgca
    32761 tggtcggcag cagcgcaccg agcgcgatga tgaagaacat caccttgttc agacgttgta
    32821 gcgacacctt ccagcccaga cgttcaaaca gtgccggagc aaactccaga gccatcacgc
    32881 cgatgtagat ggtcatacag accgccgtct cgaacagtac cgagttcacg ttgaagtgac
    32941 ccggaatgta gaagtacggc aggttccagt agcgacccac gtcgatagtg atcgacaagc
    33001 cacccagtga gtaaccaaac agactcgcca acagcgccgg acgcaccagc ggatggtatt
    33061 gcccacggtt aaagacgtat accgcccacg ccagcgccca gccgccacag gcaaagccgg
    33121 tgccaatcag caggtcaaac gcgatccaca cgccccacgg gaagccgccg ttcaggtcag
    33181 agacagagcc cagaccgaac accagacgct tcacaatcag gagcatacag atgacgatta
    33241 acggtccaaa aatcatgacc ggtttactga tgattttgcc gcccagcggt tgtggatcat
    33301 gactcatgat cgtctcctcc gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg
    33361 gttaagcccg ccagcacagc cagtggtagc atcatgcctt tatacagggt gtgttgaata
    33421 ttttcggaac gcgcaccggt agaaagatcg tccagtttcg gcaggtcgag attttcataa
    33481 ggcacacccg tcagtaccag aacctgagta ccgccgccct ctttctcgcc gtacagatgc
    33541 ggataatatt tcggcaccgt atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga
    33601 tagtggtatt cgctgccagg cttcagcgcc agacgttttt tcgcctccgc catcagctct
    33661 tcacgcgtac cgaaaatcac cgcgcccgcc gggcacactt ctacgcagcc aggcagaccg
    33721 cctttatcga gacgttccac acctttctgg ttgcacagct cgcacttatg cagcgcacca
    33781 aacgggttgt tgtagtcgta cttcggcacg ttgtacggac aggcgaccat gcagtaacgg
    33841 caaccggtgc acacgtcttt gtcgtaatgg acaatgccgg ttttcggatc ttttttcagt
    33901 gcagagaccg ggcacacaga gacacagttc ggatcgacgc agtgcataca ctgtttctta
    33961 atgtacgcgt agccgttctc ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc
    34021 tgaatgatgt tattggtata cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct
    34081 tgcgggttac gttcagggaa gttgatatcc tgacacttgg tgacgcaagc ctggcagcct
    34141 acgcacaagg tcgagtcgta caacatcccc agcgatcccg gaattggcgg gcggttttca
    34201 gcagccgcat gactgacaga cggcagcgcg cccgtcagca atgccccgca ggaggctgct
    34261 ttaataaaat tacgtctgtt cacggttatt ctccccgtga gtcagcgtta tctttctttt
    34321 gctgacgacc cagttcacgc accgccatca cgctgacacc ggcaaccagc ccaaccacac
    34381 cgccgagcaa accaatagcg cctgcagaga cgttgccgcc ctctttagcg ttaacatccg
    34441 gtttctgtga acgcggagtc tgattttcga cgttggcaag ctgatggatg cctttatgga
    34501 agccgatacc tttttcgtta cagccatagc acgggtgacc aatcgccacc ggccacacac
    34561 cgccaacatc gcagaattgc agcgttgagc agttgccgta agtttctggc cctttacagc
    34621 cgaggtggta caggcaccag ccttcgcggt ggccttcatc accgaactct ttggcaaaac
    34681 gaccagcatc gaagtgcggg cgacgttcgc agtgttcgtg aatcagacgg ccataggcga
    34741 aggtcggacg gtttttgtca tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa
    34801 cggtcgcgag gaagttgtac gggttcggcg ggcagcccgg aatattgata acggttttgc
    34861 ctggcagaac ttcttgcaag ctgactgcgc cagttgggtt aactccagct gcggcaacac
    34921 cgccccacgc ggagcaggaa ccaatagcga taatggctgc tgcgccttcc gccgctttgc
    34981 ggatgtgatc cacaatcggc tcaccggcaa ccatgcaata aataccgtta tcttttaatg
    35041 ggatggaacc atccaccact aacacatact gccctttgta cttctcgaga gcgttatgtt
    35101 tgttctcttc gacctgatga ccgaaggcgg cggaaagcac ttcgtgatat tccagagaga
    35161 tagtctccag cacgaggttt tctaccgttg gatgcgttgc acgaagcaga gattccgtac
    35221 aaccggtgca ctcctgcgcg ccaatccaga taactggcgg acgctgcggg ttagtaaccg
    35281 attcggccat ctctgcagcg gctttgctac ttaaccccat ggtggcggct aatgctgcac
    35341 aaagcttcat gaaatcacga cggttaatgc cgtgagaatg gatgagggtg ttatctccag
    35401 tcatttatag ttattccgtt gcgaggacct ggcatatatt ttgcttcaat cgcaaaatca
    35461 ataatgcgat cgatgcgcca tttaccacac atttattatg gttatcgata tcatgatact
    35521 gcggcggggc gctaaaacga agggaagaag cattagtgta attaattaaa gcaagataat
    35581 acgtatgttt gatcaatttt cgaggcgata accggccata aaaaagcgca acgtagaaca
    35641 ggaattattc tcacgaaaga aatccattac catctcttta tccagtccat agcggcgcgt
    35701 cagaataccc gcttcccagg cgctaagctg gcgatctccg gtcttttcgt acatccggtg
    35761 tgagtagcct aatacaaaac cgcgtttata atcggcacag aattttgtca cattaaccgc
    35821 actgtcggca tgcgttgctt ttaatcccgc cattaagcct ttaccaaaat ggttattcat
    35881 cgttcacccc attcgttata tatcgccata tatagcgata ttttaggcaa agaacgactt
    35941 ttgcgcacgc tattttttca tacttgttta agacgtaatc ccgtaagaga taaacctatc
    36001 aggagagata tttatcgaac catttcagtg tcctttgcca ggcaagatcg gcggcagatt
    36061 tgtcataacg gggcgtggaa tcattatgga atccgtgatt aacccccgga tagatataag
    36121 cctcataaac cttattattg tctttcaacg ccgcctcgta aggaggccag ccctcgttga
    36181 ttcgggtgtc cagttccgcg aagtggagta gtaaaggcgc ttcaatcttc gccacatcgg
    36241 cagtgggtgc ctgacgacca taaaacggca ccgcgcaggc cagttccgga tacgcgacag
    36301 ccgccgcgtt cgatacgcca ccgccatagc aaaatccggt aatacccact ttacctgtcg
    36361 cttgcggata gcgttgcata aactcaattg cggcaaagaa atcattcatc agtttggttg
    36421 gatcaacctg ctgttgcagc tcacgacctt tatcatcatt tcccggataa cctccaacgg
    36481 aacttaatcc gtcaggtgcc agggcgatat accccgcctt cgccactcgc cgtgccacat
    36541 cttcgatata cggattcagt ccacgattct catgcaccac ccccacggct ggcgttttgc
    36601 cgctcatttt tgcgggtttc accagataac cccgtacctc gccgtgacca tttggcgaag
    36661 gatacgtgat gtactcagca acaatctccg ggtcggtaaa ctctacctga gtcgccagcg
    36721 cataatttgg cttgagcaaa tcaaacaacg ctaatgccgt catcccgccc accgcatact
    36781 tcgccgcaag gttgaggaac tcacgtttcg aaattttccc gtgaacgtaa tagtcgtagt
    36841 aatccaacaa ctcttgtggg aaatctttgg cggttaaacg cggcatcgtt gcattcctca
    36901 actggtgttt ttttaagcaa agcataagcg cgtgtttttg cccagttttt cgtcactctg
    36961 tgagccagac tacgggatac gcgctggcga atcgctaaac tagaaacatt gtttcgaaat
    37021 tgaacggtgg aaaggagagg tcatggtctg gttagcgaat cccgaacgtt acgggcagat
    37081 gcagtaccgc tattgcggaa aaagtggttt acgcctgccc gcgttatcgc tcggtttatg
    37141 gcacaatttc ggtcacgtta acgcgctgga atcacagcgt gcaatcctgc gtaaagcgtt
    37201 tgatttaggc attacgcact ttgatttagc caacaattac gggccgcctc caggaagcgc
    37261 agaagagaac tttggtcgcc tgctgcgcga ggattttgct gcttatcgcg atgaactgat
    37321 tatctctacc aaggctggct acgatatgtg gcccggcccg tacggctctg gcggttcacg
    37381 taaatacctg ctcgccagcc tcgaccaaag cctaaagcgt atggggcttg agtatgtcga
    37441 tatcttttac tctcatcgcg tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct
    37501 ggctcatgcg gtacaaagcg gtaaggcgct gtatgtcggg atctcctctt actcgccaga
    37561 gcggacgcaa aaaatggtcg agttgctgca cgagtggaaa attccgcttt taattcatca
    37621 accttcgtac aatttactga accgctgggt ggataaaagc ggcctgctgg ataccctgca
    37681 aaataacggc gtgggctgta ttgcctttac tcctctggct cagggattgc tgaccggaaa
    37741 atatctcaac ggcattccgc aagattcacg gatgcatcgt gaagggaata aaattcgtgg
    37801 tctgacgccg aaaatgctta ccgaagccaa cctcaacagc ctgcgcttat tgaatgaaat
    37861 ggcacagcag cgtggacaat caatggcgca aatggcgtta agctggttgc tgaaagatga
    37921 tcgcgtgacg tcggtattga ttggtgccag ccgcgcggag caactagagg agaacgtgca
    37981 ggcgctgaat aatctgacat ttagcaccga ggagctggcg cagattgatc agcatatcgc
    38041 cgatggcgaa ctgaatctgt ggcaggcgtc gtccgataaa tgacctgtta ataacgggcg
    38101 cgcgaaccgc gcccgaatca gatcagtgat tatgacgagt cagtcggtca agatagccca
    38161 tcacaaatgc agaaagcaca aacgtcagat ggataatgac gtaccacatc agtttgttat
    38221 ccgggacatt tttcgcatcc ataaagacgc gcagtaagtg aatggaagaa attgccacaa
    38281 tcgacgctgc tactttgttt ttcagcgacg ttgcgtccat tttccccagc cagttcagct
    38341 tctctttgtt ctcggagata tccagctgtg agacgaaatt ctcataaccg gaaaacatca
    38401 ccatcaccag caaaccgcca accagcgtca tatccaccag cgacagcaac acgaggatca
    38461 aatctgattc cgccatcgag aagatattcg gcagtacgtg aataatctcc tggaagaact
    38521 tcagcgccag ggcaactaac gccagcgaaa ggccaaagta cacgggggca agcagccagc
    38581 gagaagcata cattgcattt tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa
    38641 cgggcaacag tatatcgcaa ttgagtaaac agcacgcaac agcaaatcaa catatacgtt
    38701 aaacctttgt cgcataattc tgaggtttag ggatcttcct ggttccctta tcaacgcatc
    38761 gagagcgtct acccttaaag gacgttgaac ctgaagggag aaaaacgatg tctcatttaa
    38821 aagacccgac cacgcagtat tacactggtg aatatcccaa acagaaacaa ccgacgccag
    38881 gcatccaggc gaagatgaca ccgataccgg attgcggcga gaaaacctat gttggtagcg
    38941 ctcgcctgaa agatcgtaaa gcactggtga cagggggcga ttccggaata ggtcgcgctg
    39001 ccgccatcgc ttacgcgcga gaaggggctg acgtggcgat cagttatctt cccgtggaag
    39061 aagaagacgc tcaggatgtg aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc
    39121 tgccaggcga tttaagcgat gagaaattcg cccgttcgct ggttcacgaa gcgcacaagg
    39181 cgttaggcgg gctggatatt atggcgctgg tcgccgggaa acaggttgcc attccggata
    39241 ttgcagacct caccagcgaa cagtttcaaa agacctttgc cattaacgtt ttcgcgctgt
    39301 tctggctaac ccaggaagcg atccccctgc taccgaaagg tgcaagtatc atcaccactt
    39361 cgtcaatcca ggcataccag ccaagtccgc atttactgga ctatgcggct acgaaggcgg
    39421 cgattctgaa ctacagccgt ggcctggcaa aacaggtcgc ggagaaaggt attcgggtga
    39481 atattgtcgc gccaggccct atctggacgg cactgcaaat ttccggcgga caaacgcagg
    39541 ataagatccc gcagtttggt cagaaaacgc cgatgaagcg tgcggggcaa ccggcggaac
    39601 tggcccctgt atatgtttat ctggcaagtc aggagtcgag ctacgtcacc gcagaagtgc
    39661 acggcgtgtg cggcggcgag catttaggtt aaaaaaatgc ccggttgtga aaagcaaccg
    39721 ggcatcattg tgaattcact cttacctgat gtggcgaaaa cgccttatcc gcctacaaac
    39781 gcatgaagat tcaacatgtt gcaatagatt ttgtaggcct gataagcgta gcgcatcagg
    39841 cattgagcac ctaatgccgg atgcgtcgtg aacgccttat ccggcctaca aacgcacgaa
    39901 gattcaacat gttgcaatag attttgtagg cctgatgagc gtagcgtatc aggcaattct
    39961 actttacttc gctttggcgg tttcttcgcc gaccagacct atcttcaggt aacccgcctg
    40021 atgcagcgta tccattacct tcatcaacgt ctcgtaatcg acggttttgt ccgctcggaa
    40081 gaagatggtg gtgtctttct tgccttcggt taacgcattc aacgccgtaa tcattgtttc
    40141 atcggtgacc ggatcgttac cgataaacat cgagttgtct gccttcaccg acagataaac
    40201 cggtttttcc gggcgcggct gcggcgtgct ggtagaagca ggcaagttca ccttcacatc
    40261 taccgtcgct aacggtgccg ccaccataaa gataatcagc agaaccaaca tcacgtcgat
    40321 aaacggcgtc acgttgatat catgcatttc gccgttatcg tcgaggtttt cgttaagatg
    40381 cattgccatc ggacattatc ctgcgcgtaa tttttgtgcg acacgaaccg gatgcgcagc
    40441 ggcgctggct tccagatcca ggtcacggct ttgcagcaac aatacctgcg ctgcaacatc
    40501 acccagcatc gctttaaagc cgccaatctg gcgtgcaaat acgttataga taacgaccgc
    40561 aggaatcgct gccacgaggc cgattgccgt tgctaacaga gcttctgcga tacccggcgc
    40621 aacgactgcc aggttagtgg tctgcgtttg cgcgatacca ataaagctgt tcatgatgcc
    40681 ccagaccgta ccaaacagac caacgaacgg agaaatcgcg ccgatggttg ccagatagcc
    40741 gttaccgcgg cccatttgac gacccactgc ggcgacccga cgctccagac ggaagctggt
    40801 acgttcttta ataccttcgt tatcgtcgct gccttctgac agttccagct cattctgcgc
    40861 ttcattgagc aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga tatcgttggc
    40921 ctggtttaag gaacgtgctt cagccagcag ttgctgctcg cgcttaaggc gacgcttctg
    40981 attgaagaac tctacgctct tactgaagaa gattgcccag gtgactacgg aggccaaaat
    41041 aagcccaatc atcacgcact taacgacaat atcggcgtgc tgatacatac cccagacgga
    41101 aaggtccgtc tgcattaaat tattacccac tgtgtatctc caggacgcaa gtcacaaaat
    41161 ctgcgcataa taatatcaaa acgacgtcga attgatagtc gttctcatta ctatttgcat
    41221 gctgccgtac ctttgctttc ttttccttgc gtttacgcag taaaaaagtc accagcacgc
    41281 catttgcgaa aattttctgc tttatgccaa ttcttcagga tgcgcccgcg aatattcatg
    41341 ctagtttaga catccagacg tataaaaaca ggaatcccga catggcggac aaaaagcttg
    41401 atactcaact ggtgaatgca ggacgcagca aaaaatacac tcttggcgcg gtaaatagcg
    41461 tgattcagcg cgcttcttcg ctggtatttg agagtatgga agccaaaaag cacgcgacgc
    41521 gcaatcgcgc caatggcgag ttgttctatg gacggcgcgg aacgttaacc catttttcct
    41581 tacaacaagc gatgtgtgaa ctggaaggtg gcgcaggctg cgcgctattt ccctgcgggg
    41641 cggcagcggt tgctaattcg attcttgctt ttgtcgaaca gggcgatcat gtgttgatga
    41701 ccaacaccgc ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc aaactgggcg
    41761 taacgacatc gtggtttgat ccgctgattg gtgccgatat cgttaagcat ctgcagccaa
    41821 acactaaaat cgtgtttctg gaatcgccgg gttccatcac tatggaagtc cacgacgttc
    41881 cggcgattgt tgccgccgtg cgcagtgtgg tgccggatgc catcattatg atcgacaaca
    41941 cctgggcagc cggtgtgctg tttaaggcgc tggattttgg catcgatgtt tctattcagg
    42001 ccgccaccaa atatctggtt gggcattcag atgcgatgat tggcactgcc gtgtgcaatg
    42061 cccgttgctg ggagcagcta cgggaaaatg cctatctgat gggccagatg gtcgatgccg
    42121 ataccgccta tataaccagc cgtgacctgc gcacattagg tgtgcgtttg cgtcaacatc
    42181 atgaaagcag tctgaaagtg gccgaatggc tggcagaaca tccgcaagtt gtgcgagtta
    42241 accaccctgc cctgcctggc agtaaaggac acgaattctg gaaacgagac tttacaggca
    42301 gcagcgggct attttccttt gtgcttaaga aaaaactcaa tgatgaagag ctggcgaact
    42361 atctggataa cttcagttta ttcagcatgg cctactcgtg gggcgggtat gaatcgttga
    42421 tcctggcaaa tcaaccagaa catatcgccg ccattcgccc acaaggcgag atcgatttta
    42481 gcgggacctt gattcgcctg catattggtc tggaagatgt cgacgatctg attgccgatc
    42541 tggacgccgg ttttgcacgg attgtataac attgccactt ttggacaatt ttgcagacat
    42601 tttattgtga aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg acgattcagg
    42661 agtacaatag gcagataaag gcttaaacgc tgttccacag gaaagtccat ggctgttatt
    42721 caagatatca tcgctgcgct ctggcaacac gactttgccg cgctggcgga tcctcatatt
    42781 gttagcgttg tttactttgt catgtttgcc acgctgtttt tagaaaacgg cctgctgccc
    42841 gcctcatttt tgccaggcga cagcttgttg atactggcag gcgcattgat tgcccaaggg
    42901 gttatggatt ttctgcctac gattgcgatt ctgaccgccg cagcaagtct gggctgctgg
    42961 ctaagttata ttcaggggcg ctggttaggg aataccaaaa cggtgaaagg ctggctggca
    43021 cagcttcctg ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca cggtctgctg
    43081 gcgctgctgg ctggacgttt tcttgcattt gtccgtacgc tgctgccaac catggcggga
    43141 atttccggtc tgccaaaccg ccgcttccag tttttcaact ggttaagtgg attgctgtgg
    43201 gtcagcgtgg taaccagttt tggctatgcc ttaagtatga ttccgttcgt taaacgccat
    43261 gaagatcagg taatgacgtt cctgatgatc ctgccaattg ccttgttaac cgctggcttg
    43321 ttaggcacgc tgtttgtggt gattaaaaaa aaatactgta acgcctgacg attttccccg
    43381 ttcccggctg ctgtaccggg aacgtattta attcccctgc attgcccgca ttcttgccgc
    43441 atcttccccc ggcgtcacac cgaagtaacg tttaaactca cggctaaatt gcgatgcgct
    43501 ttcatagccg acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga tgatcatcat
    43561 ccgcgcctta tgcagacggt aattcttcaa atactgcaac ggcgaggtgc tggtgacaga
    43621 cttaaaatta tggtggaacg ccgatacgct catgttggct tctgccgcca gttgctcgac
    43681 gctcaggttt tcggtgtatt tattctcaat tcgtttcagc acgcggctaa tcagactgaa
    43741 gtgagtctgg cgactgacca gcgccagtaa cgcgccgccg caaggtccgg tcagcacgta
    43801 gtacagaatt tcgcggatga tctgtttgcc gagaatacgc gcatccagtg gtcgctccat
    43861 cacgtcgagt aaccgctccg ccgcgaataa aatctcttct gataacgtgg cggagttaat
    43921 cccgctggct gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca tcaacagttc
    43981 ctgtaactgc aaaatatcga cattgagacg caaccctgcc agcggcacct ctgacgtggc
    44041 ataggtttcg cactcaaacg gcaacggcac cgtcagcagc aggtattcat tggcatcata
    44101 acgaaacacg cg
//
LOCUS       DADWTH010000024        43914 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.024, whole genome shotgun sequence.
ACCESSION   DADWTH010000024 DADWTH010000000
VERSION     DADWTH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.024"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..1261)
                     /locus_tag="JNY02_001383"
                     /pseudo
     CDS             complement(1..1261)
                     /locus_tag="JNY02_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            1655..3025
                     /locus_tag="JNY02_001384"
     CDS             1655..3025
                     /locus_tag="JNY02_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9432986.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAITAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3207..5371
                     /locus_tag="JNY02_001385"
                     /pseudo
     CDS             3207..5371
                     /locus_tag="JNY02_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
     gene            5424..7154
                     /locus_tag="JNY02_001386"
     CDS             5424..7154
                     /locus_tag="JNY02_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9432987.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQYVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(7214..7705)
                     /gene="yajQ"
                     /locus_tag="JNY02_001387"
     CDS             complement(7214..7705)
                     /gene="yajQ"
                     /locus_tag="JNY02_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBB9432988.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7873..8784
                     /gene="panE"
                     /locus_tag="JNY02_001388"
     CDS             7873..8784
                     /gene="panE"
                     /locus_tag="JNY02_001388"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBB9432989.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARTHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8747..9337
                     /gene="yajL"
                     /locus_tag="JNY02_001389"
     CDS             8747..9337
                     /gene="yajL"
                     /locus_tag="JNY02_001389"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBB9432990.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(9391..10839)
                     /gene="thiI"
                     /locus_tag="JNY02_001390"
     CDS             complement(9391..10839)
                     /gene="thiI"
                     /locus_tag="JNY02_001390"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBB9432991.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            11045..11287
                     /gene="xseB"
                     /locus_tag="JNY02_001391"
     CDS             11045..11287
                     /gene="xseB"
                     /locus_tag="JNY02_001391"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBB9432992.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            11287..12186
                     /gene="ispA"
                     /locus_tag="JNY02_001392"
     CDS             11287..12186
                     /gene="ispA"
                     /locus_tag="JNY02_001392"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBB9432993.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            12211..14073
                     /gene="dxs"
                     /locus_tag="JNY02_001393"
     CDS             12211..14073
                     /gene="dxs"
                     /locus_tag="JNY02_001393"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBB9432994.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTICVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            14128..15102
                     /gene="yajO"
                     /locus_tag="JNY02_001394"
     CDS             14128..15102
                     /gene="yajO"
                     /locus_tag="JNY02_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBB9432995.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(15156..15674)
                     /gene="pgpA"
                     /locus_tag="JNY02_001395"
     CDS             complement(15156..15674)
                     /gene="pgpA"
                     /locus_tag="JNY02_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBB9432996.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15652..16629)
                     /gene="thiL"
                     /locus_tag="JNY02_001396"
     CDS             complement(15652..16629)
                     /gene="thiL"
                     /locus_tag="JNY02_001396"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBB9432997.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(16707..17126)
                     /gene="nusB"
                     /locus_tag="JNY02_001397"
     CDS             complement(16707..17126)
                     /gene="nusB"
                     /locus_tag="JNY02_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBB9432998.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(17146..17616)
                     /gene="ribE"
                     /locus_tag="JNY02_001398"
     CDS             complement(17146..17616)
                     /gene="ribE"
                     /locus_tag="JNY02_001398"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBB9432999.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17708..18808)
                     /gene="ribD"
                     /locus_tag="JNY02_001399"
                     /pseudo
     CDS             complement(17708..18808)
                     /gene="ribD"
                     /locus_tag="JNY02_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     gene            complement(18812..19261)
                     /gene="nrdR"
                     /locus_tag="JNY02_001400"
     CDS             complement(18812..19261)
                     /gene="nrdR"
                     /locus_tag="JNY02_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBB9433000.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            19412..19951
                     /locus_tag="JNY02_001401"
     CDS             19412..19951
                     /locus_tag="JNY02_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBB9433001.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            20250..21134
                     /gene="tsx"
                     /locus_tag="JNY02_001402"
     CDS             20250..21134
                     /gene="tsx"
                     /locus_tag="JNY02_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBB9433002.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKIRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(21311..21658)
                     /locus_tag="JNY02_001403"
     CDS             complement(21311..21658)
                     /locus_tag="JNY02_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBB9433003.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21787..22758)
                     /gene="secF"
                     /locus_tag="JNY02_001404"
     CDS             complement(21787..22758)
                     /gene="secF"
                     /locus_tag="JNY02_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBB9433004.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22769..24616)
                     /gene="secD"
                     /locus_tag="JNY02_001405"
     CDS             complement(22769..24616)
                     /gene="secD"
                     /locus_tag="JNY02_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBB9433005.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24644..24976)
                     /gene="yajC"
                     /locus_tag="JNY02_001406"
     CDS             complement(24644..24976)
                     /gene="yajC"
                     /locus_tag="JNY02_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBB9433006.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24999..26126)
                     /gene="tgt"
                     /locus_tag="JNY02_001407"
     CDS             complement(24999..26126)
                     /gene="tgt"
                     /locus_tag="JNY02_001407"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBB9433007.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(26181..27251)
                     /gene="queA"
                     /locus_tag="JNY02_001408"
     CDS             complement(26181..27251)
                     /gene="queA"
                     /locus_tag="JNY02_001408"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBB9433008.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            27344..27917
                     /gene="acpH"
                     /locus_tag="JNY02_001409"
                     /pseudo
     CDS             27344..27917
                     /gene="acpH"
                     /locus_tag="JNY02_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(27922..29739)
                     /gene="malZ"
                     /locus_tag="JNY02_001410"
     CDS             complement(27922..29739)
                     /gene="malZ"
                     /locus_tag="JNY02_001410"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBB9433009.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYLFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQSEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29895..31268)
                     /gene="proY"
                     /locus_tag="JNY02_001411"
     CDS             complement(29895..31268)
                     /gene="proY"
                     /locus_tag="JNY02_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBB9433010.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKIAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(31344..32663)
                     /gene="brnQ"
                     /locus_tag="JNY02_001412"
     CDS             complement(31344..32663)
                     /gene="brnQ"
                     /locus_tag="JNY02_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBB9433011.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(33070..34365)
                     /gene="phoR"
                     /locus_tag="JNY02_001413"
     CDS             complement(33070..34365)
                     /gene="phoR"
                     /locus_tag="JNY02_001413"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBB9433012.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(34423..35112)
                     /gene="phoB"
                     /locus_tag="JNY02_001414"
     CDS             complement(34423..35112)
                     /gene="phoB"
                     /locus_tag="JNY02_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBB9433013.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            35302..36504
                     /gene="sbcD"
                     /locus_tag="JNY02_001415"
     CDS             35302..36504
                     /gene="sbcD"
                     /locus_tag="JNY02_001415"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBB9433014.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            36501..39644
                     /gene="sbcC"
                     /locus_tag="JNY02_001416"
     CDS             36501..39644
                     /gene="sbcC"
                     /locus_tag="JNY02_001416"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBB9433015.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADMKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVELDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            39770..40951
                     /gene="araJ"
                     /locus_tag="JNY02_001417"
     CDS             39770..40951
                     /gene="araJ"
                     /locus_tag="JNY02_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBB9433016.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(41094..42002)
                     /gene="mak"
                     /locus_tag="JNY02_001418"
     CDS             complement(41094..42002)
                     /gene="mak"
                     /locus_tag="JNY02_001418"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBB9433017.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            42127..43038
                     /gene="rdgC"
                     /locus_tag="JNY02_001419"
     CDS             42127..43038
                     /gene="rdgC"
                     /locus_tag="JNY02_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBB9433018.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(43116..43571)
                     /locus_tag="JNY02_001420"
     CDS             complement(43116..43571)
                     /locus_tag="JNY02_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433019.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAAGRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(43568..>43914)
                     /locus_tag="JNY02_001421"
     CDS             complement(43568..>43914)
                     /locus_tag="JNY02_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HBB9433020.1"
                     /translation="QVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASL
                     LTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKI
                     RSEARHRLEEKQ"
BASE COUNT        11437 a        11353 c        10679 g        10445 t
ORIGIN      
        1 cccccccctt ttagctcaag cgtcaactgc aaattagtta tactactaat tttatctgca
       61 ttaatttcaa tactgacatc gcatattttg ggtttttcat ttgcgttaaa gccataggtc
      121 ccgtaatgtt ttaacgtgat gtaaggtaaa gtatcaccct gtgtttctcg ggttttctga
      181 tcttgattac aacgggttaa atattcatcc ttattgaatt tcattgaata tttccccgca
      241 cctttttcgt aataatccca taccttatca caatcaagcg tttcacctaa tccgttgtaa
      301 tacatccaac ttaaccacaa tgcggctttt ttgccccttg ctgttcagcc ttgtcaaaaa
      361 gcatgctcgc tttagaataa tcaacctcac cggtcaaacc agaatagtat atattcccca
      421 gggccactaa tgctttttcg tcattcttat ttgcagctaa ttcgtaccat tttttggctt
      481 cgacagcatc aatctttaaa ccagcactgc cattttcgta ggtatcgccc atggtatact
      541 gcgccatcgg ataacctttt tctgccgcag tacgaaacca ttgcagtgct ttctttatgg
      601 tgtctaaaga acgtctatca tcagaaggtg aaaaatcatc gagataaaga tttccgacaa
      661 tataatccat ttcggggtcg ccttcctgcg cccatagagt cgccagacgc agaatttcag
      721 gtttactgaa accaattcgc ccccaaaaaa gcaaatcatt ctcgggtgcc tgaccgacat
      781 aacgactgac aaattcaatt aaactcgccc gtttcgtttt gtctgtctca gccccggatt
      841 tcaattcata ggcctgattg attattttat tcatcaaatc aggcggaata ttaagatcgt
      901 cagttttcga cttactggca tacttctgtg ctcgtgtcac atctagggtg acgcctttac
      961 catattgaaa gaaataacgt gcattataac aaccgtattc gcccccctgg ttacaggctt
     1021 tctcatacca ctgaatcgcc tttgggatgt caacagatac tgtcactccg tgttcataaa
     1081 taaatcccag tagcactagc gcctgcgtgt tattggtatc agctaatttt ttcagttctt
     1141 ttaacgccgt ctgatcttta ttgacaatac gttcatacag ttgatcaata tctgttgaag
     1201 catgacaaaa agaagccaga gataaaccca atgataataa aataacttta ccagaccaca
     1261 tacattcctc cttgatatat tcaaccacca aacatccacc tcatgaataa atacgctgcc
     1321 ataacggccc aggtaataag taaacattta agctgaaaca gcataaccag tgcctccaac
     1381 gcttctattt attgcgataa aataacacca tcctttgata aaaagtcaca cgcccatcaa
     1441 taaatccaaa aataatgtca ttagttgcag gataaaatca tttgagcttt tcatttgtta
     1501 tatttaacgc atgatgtatt aatggcaaga atatgagaga aactcatacc tgacgtaaaa
     1561 atcactctct tatgtaatat ttacttatta acactctgtt tacctaacat gcgctccgca
     1621 ctacactatg tgggcaattt tttaagaggt ggtaatgaac gattataaaa tgacgccagg
     1681 tgagcggcgc gcgacctggg gtttagggac cgtattttca ttgcgcatgc tgggcatgtt
     1741 tattgtactg ccggttctga ctacgtacgg tatggcactg caaggcgcca gcgaagcatt
     1801 aatcggtatt gccattggta tttatggtct gacccaggcc gtttttcaga ttccgtttgg
     1861 cctgctttcc gaccgcattg gtcgaaaacc attaattgtc ggtgggctgg cagtgtttgc
     1921 cgccggtagc gttatcgctg cgctttctga ctccatctgg ggaattattc tgggccgggc
     1981 gctacaaggc tccggtgcga ttaccgctgc cgttatggcg ctgctttccg atctcacgcg
     2041 cgaacaaaac cgtaccaaag caatggcgtt tatcggcgtg agctttggca ttacctttgc
     2101 cattgcgatg gtgcttggcc cgatcatcac tcacaaactt gggctgcacg cgctgttctg
     2161 gatgatcgct attctggcaa cgaccggcat tgcgttgacc atttgggttg tgcccaacag
     2221 tagcactcac gtacttaatc gtgagtccgg aatggtgaaa ggcagtttca gtaaagtgct
     2281 ggcggaaccg cggctgctga aactcaactt tggcattatg tgtctgcata ttttgctgat
     2341 gtcgacgttt gttgccctgc ccggacaact ggctgatgcg gggttcccgg cggctgaaca
     2401 ctggaaggtc tatctggcga caatgctaat cgcctttggc tcggtcgtgc ctttcattat
     2461 ctacgctgaa gttaagcgca aaatgaagca agtctttgtc ttctgcgtcg ggttgatcgt
     2521 ggttgcggaa attgtgttgt ggaacgcgca aacgcagttc tggcaactgg tggtcggcgt
     2581 gcagcttttc tttgtggcgt ttaatttgat ggaagccctg ctgccctcac ttatcagtaa
     2641 agagtcgcca gcaggttaca aaggtacggc gatgggtgtt tactccacca gccagtttct
     2701 tggcgtggcg attggcggtt cgctgggcgg ctggattgac ggcatgtttg acggtcagcg
     2761 ggtatttctc gctggcgcaa tgctggccgc agtgtggctg gcagtcgcca gtaccatgaa
     2821 agaaccgccg tatgtcagca gtttgcgcat tgaaatccca gaaaacgtaa tggcagatga
     2881 aaaactggaa aaatgcttac tgacaacgcc tggtgtcaaa gatgtactgc tggcgaagtt
     2941 agaacattgc gtctatgtga aagtagacag taaaatcacc aatcgttttg aaatagagcg
     3001 agtaatcaac gaagtatctt attaaataat aagcacggtg ctattcatga aaaataaaat
     3061 agcaccgtac ttcgtaacac cataatcctt taaaccctcc attattaata cttcgcttaa
     3121 ttccatataa ctggacgatt acattagaag taaactatac tttttatgaa atatcaggga
     3181 taaattattc acatggaaaa tacatcatgt acaaatcatt atttactatt atcattctac
     3241 tgtatagcgc cagttcatat gcaaaaagcg aatcccctcc cgcaagcact ataactttca
     3301 ttgcacaaat cgatcatgtt gagctaaata aaacagcatt atctcatgat atgaaactcg
     3361 ttcttgccga tcgtttttat ttcaaaacca gtaatccttg cgaaacaaat tcaatttctg
     3421 ctcgaccaga atcaatggat attactgaca aagcctatgc caacaaatta aaaaaattag
     3481 aaactccttt tcttgatgat cgctatttct ttatgaccat caatcagtgt gacggtaatg
     3541 gcactcccat gctgacagac ataaaagtat gtacaacaga attatgtggt gctgaatata
     3601 caaagaaaat gtctactgta tggctcgatg aaactttaaa accaactgct aaacgtctgg
     3661 caatttattt tattcaatct cctttaccct atgacgagga gaaaaaacaa tggaaagtca
     3721 ccggatggta tatctcatca actgataatc agtcttcaac taaacagatt gcatttgaag
     3781 gatatacaga cacagataac ttctccgatt ctaaatttat ttcttcattt aaatcctggt
     3841 ttgaaagtgg aaagataaaa aacattgcta actataatcc aaaaggagaa agagatggaa
     3901 aatacgaaag ttattacaat agcaatggta acgtctatga atctctaagt ttttctaatg
     3961 gtcttatcaa cggtgagttt attacatatt atgaaaatgg ggccattgaa agtaaacgtc
     4021 attttaataa cgacaaaatt aaagatggtg aatgccctca tttttatgaa gatgggaaaa
     4081 tcaaacagca acatagctat ctgaatagta aactcgatgg tcctgcatat gaatattatc
     4141 ctgatggcaa actcatgcag gaaaatttct atcagcaaag tgtgttgatt ggaaacgata
     4201 ccagttacta ccaatccgga aaaataagct ctatacacaa tagaaattcc cgtggtcagt
     4261 atgatggagc taacgaacgt tatagtgaag agggtaagtt acagtctaaa tccgtctata
     4321 aagacggaaa acaaatttct gttcaaacct ggtatgaaaa tgggcagaaa gaagaggaga
     4381 aacattttga tgagcaaggc caattaaata gtctggttaa gcaatggtat aaaaatggta
     4441 atcttgcaaa atcacaaaac tataaacatg acatactcga tggtaacagt gaagagtggt
     4501 acgaaaatgg cattcctgaa agtttgtacc cttataagaa tggaaaaacc gacggcattg
     4561 ccaaatcatg gaataagtat ggtaaattaa cctattcaat cgaatacaaa aatggcgtag
     4621 agaatggggc atatcgtaac tggagcaaaa acacaggtaa actaaccaaa gaaaccctgt
     4681 acgtcaatgg tatcagacag ggagtggaaa aagagtttaa cgatcgcaca ggtaaactat
     4741 taacctccac gcaatatgtt aataataaaa gacatggtac ggaggaaact tatgatcaaa
     4801 acggaataaa atatattaca tgctatcaaa atgatcagaa attatcgtct ttagacaatc
     4861 caacacaaat aaaagataat gcaacaacag gcgattccag tgctcaattt gcattaggga
     4921 aatacgaatt catctgtgca aatatagatg aagggattaa atggttaacc aaatctgcag
     4981 agcaaaaaaa acactgatgc gatatacttt ttagctacag cctacaaagg caatggtata
     5041 cctgcaaaca atgaaaaata cctagcatat ctgcaacaag ctgcaacaag ctgcaacact
     5101 gggcagtagt aatgcccagg cggaaattgg ctatttatat ctcatcggaa aagaactacc
     5161 acagaatctt cctgatgctg gtgtctggtt caaaaaagca gctgcacaag gtaattttgc
     5221 cgcacatttc tatctgggaa ggatgtatca gaatggtgac ggcgttgaga gaaatatgga
     5281 aaaagccagg tttcatctat ctaatgcggc agaaggtgga atagaacctg cgcttaaagc
     5341 attaaatgaa cttgaacatc aaacaaaata aagcgctcag ggaccacgta aggttcatca
     5401 aacagaaaag gatatagttt gcgatgaaag cgaaatctac tttgttatta atgattttat
     5461 ctgtttttta ttctcatcag gttttatcca gcgaccccac tcagccggat gtttattctg
     5521 tcgtcgagaa aaaactaaac tctgctttac ctctggaaga aaataccacc ttcagatcgc
     5581 aggctgaatg gtttaatttg caatatgaat tattagtatc tggctaccct gaacgtgctt
     5641 acaatttact gagcaaactt gagacagaat cgaaaaacgc taaatattat ctggatttaa
     5701 ctgcctccag agtcgctgaa gaagaagcac cagagacagc cttaactttt ttatcaaaaa
     5761 ttgggttaaa taaaccggct actcttacat cctttgagag ctatatcaat agctggataa
     5821 aaagcaaaca acccgaaaag gctatgcagc ttctcagcct cgatgagaat gccctgaatt
     5881 attatttacc gactgtgtta gtcgcatatc agatcgttcc tgataaggca ataagtattt
     5941 ataattctgt atatggagat aatgttgtta taccctatca gcaagtcacc atgttactta
     6001 cagttgcgga gggttatcaa acaaaaggcg atataaaaaa cgccattcat tatgccgata
     6061 aggcgctgaa tatgtttgat aaagctattg ctgtagatag cagtcgtgaa tcccattata
     6121 gtgatgagta tttgaaactc atgaatatct acgccgccaa tggcaacaaa gaaaaagcgg
     6181 tggttttatc tcaacgtcta cacaaagcca ttaccgaatc tcaatcgaat tacttaagta
     6241 ctctggaagg aattttattg ttttatagaa ataatgatat gcagcaagac tatcaaaaca
     6301 gcctttcaaa ttacatagta tttatcgatg aaatttttgc cttttcccca tcaacaagaa
     6361 gtgaattaag cctcattaat ttattaaatt cattaaatga ggtggaattg atgagaaaac
     6421 gtctggcgtt tctgatgaca gcaccagaat acgcttgtta tgatagtcag tattgttatg
     6481 aagacaaagt cagcgcatta aaaatcctgt atcgtcatca tgacaatgag ctaatggaca
     6541 aatatttcac gactcttctt aaagagatca gtaatcaacc attagaagac tgggatataa
     6601 taataagtaa tactggtgaa aaatttagtg aagtgggtct aaccaaatat gctcaacatc
     6661 tcgcatcttt cgctgaagaa ttttatataa aagagcaaaa atcacattct gaaagtgaaa
     6721 tgagaggact attttcatca cttgccgaat tatatagttt tggtaatgat actgccagcg
     6781 caaaccgagt gttccatcag tatgtccctg ccttcaatac agatcatatg attcaatatt
     6841 ttataaatgc caaagaatgg aacaatgcca gggaattatt gataaaagag gaaatgttgg
     6901 gtatgcataa tctgatattg ttggagaata tttgtatgca aaaaaatgca gaatgcatgg
     6961 cacacattac ctttacgctt aataaattaa caacacaacc agcaataaca aaaatagata
     7021 gcgtgggtaa tgagcaactt tatcagattg gcaaaatcta ccataagttg gaaattaaac
     7081 ctgagccaga gcaacaggtt ttaattcagt cgctttatga ccgggcatca ggaagcgcct
     7141 ctgcaactca ataaatgcat ggtcaaaagg gccaccacgg tggccctgac aatcagtagc
     7201 tatatccaga aagttaatcg cggaagtttt tgaactggaa cggctgaccg agatcgccac
     7261 cgcgcaccat cgccatgaca gcctgcaaat catcacgaga tttgccagtc acgcggatct
     7321 catcgccctg aatttgcgcc tgcactttca gtttgctgtc tttgatcatc ttgacgattt
     7381 ttttctgagt ggcgctctca atgccctgtt tcagtttcgc ttccacaaac caggttttac
     7441 cgctatgaac gatattttcc ggtacatcca gtgaactgcc ttcaatgccg cgcttcagca
     7501 gcttggcacg cagaatatcc agcaactgat tgacctggaa gtcggactcg ctcaacactt
     7561 tgatggtttt gctggcgtcg ttcagctcaa atgaggcttc aacgttacgg aagtcaaaac
     7621 gggactccac ttcgcgactc gcgttatcga ccgcgttacg tgcttcctga agatcaactt
     7681 cagagacaat atcgaaagat ggcatctttt cctctccctt catttttgat gcgaagcata
     7741 atacctgcaa agttaaataa ccaacagtcc tgcaccagga cgtatccctt aattgtgcat
     7801 agtcgcaaca gcatgcacaa ttaagggata gccttattga acacccggag tggttgtggg
     7861 tgaggaggaa caatgaaaat taccgtattg ggatgcggtg ccttagggca attatggctt
     7921 acagcacttt gtaaacaggg tcatgaagtt cagggctggc tgcgcgtacc gcaaccttat
     7981 tgtagtgtga atctggttga gacagatggt tcgatattta acgaatcgct gaccgccaac
     8041 gatcccgatt ttctcgccac cagcgatctg ctcctggtga cgctgaaagc atggcaggtt
     8101 tccgatgccg tcaaaagcct cgcatccaca ctgcctgtaa ctacgccaat actgttaatt
     8161 cacaacggca tgggcaccat cgaagaattg caaaacattc agcagccatt actgatgggc
     8221 accaccaccc atgccgcccg ccgcgacggc aatgtcatta ttcatgtggc aaacggaatc
     8281 acgcatatcg gtccggcacg gcaacaggat ggcgattaca gttatctggc ggatattttg
     8341 caaaccgtgt tgcctgacgt tgcctggcat aacaatattc gcgccgagct gtggcgcaag
     8401 ctggccgtca actgcgtgat taatccactg actgccatct ggaattgccc gaacggtgaa
     8461 ttacgtcatc atccgcaaga aattatgcaa atatgcgaag aagtcgcggc agtgatcgaa
     8521 cgcgaagggc atcatacttc agcggaagat ttgcgtgatt acgtgatgca ggtgattgat
     8581 gccacagcgg aaaatatctc gtcgatgttg caggatatcc gcgcgctgcg ccacaccgaa
     8641 atcgactata tcaatggttt tctcttacgc cgcgcccgca cgcatgggat tgccgtaccg
     8701 gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga gtgaatatga gcgcatcggc
     8761 actggtttgc ctcgcccctg gtagtgaaga gactgaagcc gtcaccacga tcgacctctt
     8821 ggttcgcggc ggtatcaaag tcaccactgc cagcgtcgac agcgatggca acctggcaat
     8881 tacctgctcg cgcggcgtga agctgctggc ggatgcgccg ctggtcgaag tggctgatgg
     8941 cgaatatgac gtgatcgtgc tgcctggtgg cattaaaggc acggagtgtt ttcgcgatag
     9001 cactctgctg gttgaaaccg ttaaacagtt ccaccgttcc gggcgtatcg tcgcggctat
     9061 ttgcgccgcg ccagccaccg tgctggtgcc gcacgatatc tttccgattg gcaatatgac
     9121 cggcttcccg acgctgaaag acaaaattcc cgctgaacaa tggcaggaca agcgcgtcgt
     9181 ctgggatgca cgggtaaaat tgctgaccag ccaggggccg ggtacagcta tcgactttgg
     9241 tctgaaaatt atcgacctgc tggttgggcg cgagaaagcc catgaagtgg catcacaact
     9301 ggtgatggcg gcagggattt ataattatta cgagtagtgt cggatgcggc aaacgtcgca
     9361 tctgaccaga tgcgacgtaa aaactacaaa ttacgggcga tataccttca cattgttaaa
     9421 gccttgctcg cgcagataga gcgcctgcag acggctcatc accccgcgct cacaccacag
     9481 cagccaggtt ttgttctggt cgagatcgcc aaatttggtg ctcagtttat agaacggcag
     9541 agaaaccaca tcaatccctt cgactttcag tggcttatct tcctgttcat cgacagaacg
     9601 gatatcgaga atcacgtcgt tcgggccgaa gccgttgacg gtttccactt ccaccacttc
     9661 ctgctcggtc tgctgggcga tttcgcggat atcaacgtta ttcgcttcct caaccacttt
     9721 atcgagaata ctgaagtcga acttctcttc ttccgcttca atcttcgatt taaccgcttt
     9781 caccgtcggg cttttggaga tcacgccaca atattccggc atcgtgcgag caaagtcttc
     9841 ggtgccaatc tggcgagcca ggttgatgat gtgctctttg tcgtaagaaa tcagcggacg
     9901 caggatcagc gtgtcagaga cgttatcaat caggcgcagg ttggtcagcg tctgactgga
     9961 cacctggccg agcgcttcgc cggtgaccag cgcctgtacg ccgtaacgtt cagccacttt
    10021 agatgcggca cgcaccatca tacgtttgag gataacgccc atctgaccgt cgtcgatttt
    10081 ctcgagaatt tccccgacaa ccggttcgaa attaatggcg acaaaacgca cgcggtggga
    10141 gctgccaaag cggttccaca gataatgcgc cacctgacgc acgccaattt catgcgctgc
    10201 gccgccgagg ttaaagaagc agtaatgcac gcggcagccg cgacgcatca acatataact
    10261 ggaaacaccg gagtcgaaac caccggaaat gagcgacagc acatcttcct gggtgccaat
    10321 cgggaaacca ccaatacctt cgtagcgacc tttaatcagc aggaggcgat cgtcttccac
    10381 ttccagatga acagttacat ccgggttagt cagcttcacg cgcgcggatt caatatgctg
    10441 atttaaaccg ccgccgacgt aacgttccac atcaatcgag ctaaaatcat gtttgccacg
    10501 gcgcttcacg cgtacgcaga aggttttgcc ttccagctga tcgcgatact gaaccaacgc
    10561 tttctcaaaa atatcgtgca tgtcggtaaa cggcacgtct tcgacttcga gaatatggtg
    10621 gatacccgga atacgggtca gggcgtcgcg aatagtcaga cgctggtttt catctttcgc
    10681 gcgaacttcg atgttatccc agtggcggac gacagcgagc gtctcatcat agtgctttaa
    10741 aacgttacga atgttcccgg taaggatttt tataaagcgc aagcgcacag attggctttt
    10801 gatggtgatt tccgggaaca atttaatgat aaacttcatg gcggcaatgg ttcgttggca
    10861 agccttaagc gacttgtata gggaaaaata cagcagccca cacctgcggc tgcatccagg
    10921 cgcggaagta taccactaac atcgctttgc tgcgcacatc accttaccat tgcgcgttat
    10981 ttgctatttg ccctgagtcc gttaccatga cggggcgaaa aatattgaga gtcagacatt
    11041 cattatgccg aagaaaaatg aggcgcccgc cagctttgaa aaggcgctga gcgagctgga
    11101 acagattgta acccgtctgg aaagtggcga cctgccgctg gaagaggcgc tgaacgagtt
    11161 cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa ttacaacaag ccgaacagcg
    11221 cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta acccctttta caccggacaa
    11281 tgagtaatgg actttccgca gcaattcgaa gcctgcgtta agcaggccaa ccaggcgctg
    11341 agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac catgcagtat
    11401 ggcgcattat taggtggtaa gcgcctgcga cctttcctgg tttatgccac cggtcatatg
    11461 tttggcgtta gcacaaacac gctggacgca cccgctgctg ccgtagagtg tatccacgct
    11521 tactcattaa ttcatgatga tttaccggcg atggatgatg acgatctgcg tcgcggtttg
    11581 ccgacctgcc atgtgaagtt tggcgaagca aacgcgattc tcgctggcga cgctttacaa
    11641 acgctggcgt tctcgattct aagcgatgcc aatatgccgg aagtgtcgga tcgcgacaga
    11701 atttcgatga tttctgaact ggcgagcgcc agcggtattg ccggaatgtg cggtggtcag
    11761 gcattagatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga gcgtattcat
    11821 cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt aagcgccgga
    11881 gataaagggc gtcgtgctct gccagtactc gacaagtacg cagaaagcat cggccttgcc
    11941 ttccaggttc aggatgacat cctggatgtg gtaggagata ctgcaacgtt gggaaaacgc
    12001 cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg tcttgagcaa
    12061 gcccggaaga aagcccggga tctgatcgac gatgcccgtc agtcgctgaa acaactggct
    12121 gaacagtcac tcgatacctc ggcactggaa gcgctagcgg actacatcat ccagcgtaat
    12181 aaataaacaa taagtattaa taggcccctg atgagttttg atattgccaa atacccgacc
    12241 ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag tttaccgaaa
    12301 ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag cgggcacttc
    12361 gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta caacacccca
    12421 tttgaccaat tgatttggga tgtgggacat caggcttatc cgcataaaat tttgaccgga
    12481 cgccgcgaca aaatcggcac catccgtcag aaaggcggtc tgcacccgtt cccgtggcgc
    12541 ggcgaaagcg aatatgacgt attaagcgtc gggcattcat caacctccat cagtgccgga
    12601 attggtattg cggttgctgc cgagaaagaa ggcaaaaatc gccgcaccat ctgtgtgatt
    12661 ggcgacggcg cgattaccgc tggcatggcg tttgaagcga tgaatcacgc gggcgatatt
    12721 cgtcctgata tgctggtggt cctcaacgac aatgaaatgt cgatttccga aaatgttggc
    12781 gcgcttaaca accatctggc tcagctgctt tccggtaagc tttactcttc actgcgcgaa
    12841 ggcgggaaaa aagttttctc tggcgtgccg cccattaaag agctgctcaa acgtaccgaa
    12901 gaacatatta aaggcatggt ggtgcctggc acgttgtttg aagagctggg atttaactac
    12961 atcggcccgg tggacggtca cgatgtgctg gggcttatca ccacgctaaa gaacatgcgc
    13021 gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg ttatgaaccg
    13081 gcagaaaaag acccgatcac tttccacgcc gtgcctaaat ttgatccctc cagcggttgt
    13141 ttgccgaaaa gtagcggcgg tttgccgagc tattcaaaaa tctttggcga ctggttgtgc
    13201 gaaacggcag cgaaagacaa caagctgatg gcgattactc cggcgatgcg tgaaggttcc
    13261 ggcatggtcg agttttcacg taaattcccg gatcgttact tcgacgtggc tattgccgag
    13321 caacacgcag tgacctttgc cgccggtctg gcgattggtg gatacaaacc cattgtcgcg
    13381 atctactcca ccttcctgca acgcgcctat gatcaggtgc tgcatgacgt ggcaattcaa
    13441 aagctaccgg tcctgttcgc cattgaccgc gcgggcattg ttggtgctga cggtcaaact
    13501 caccagggcg cttttgatct ctcttacctg cgctgcatac cagaaatggt cattatgacc
    13561 ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta taacgatggc
    13621 ccgtccgcgg tgcgctaccc gcgcggcaac gcggttggcg tggaactgac gccgctggaa
    13681 aaactaccaa ttggcaaagg cattgtgaag cgtcgtggcg agaaactggt gatccttaac
    13741 tttggtacgc tgatgccaga agcggccaaa gtcgccgaat cgctgaacgc tacgctggtc
    13801 gatatgcgtt ttgtgaaacc acttgatgaa gcgttaattc tggaaatggc cgccagccat
    13861 gaagcgctgg tcaccgtaga agaaaacgcc attatgggcg gcgcaggtag cggagtgaac
    13921 gaagtgctga tggcccatcg taaaccagta cccgtgctga acattggcct gccggacttc
    13981 tttattccgc aaggaactca ggaagaaatg cgcgccgaac tcggcctcga tgccgccggt
    14041 atggaagcca aaatcaaggc ctggctggca taatccctac tccactcctg ctatgcttaa
    14101 gaaattattc atggcaggag tggcagcatg caatacaacc ccttaggaaa aaccgacctt
    14161 cgcgtttccc gactttgcct cggctgtatg acctttggcg agccagatcg cggtaatcac
    14221 gcatggacac tgccggaaga gagcagccgt cccataatta aacgcgcgct ggaaggtggc
    14281 ataaatttct ttgataccgc caatagctat tccgatggta gcagcgaaga gatcgttggt
    14341 cgcgcactgc gggatttcgc ccgccgtgaa gacgtagtcg ttgccaccaa agtgttccat
    14401 cgcgttggtg atttaccgga aggattatcc cgtgcacaaa ttttgcgctc tatcaacgac
    14461 agcctgcgac gtctcggcat ggattatgtc gatatcctgc aaattcatcg cggggattac
    14521 aacacgccga tcgaagagac gctggaagcc ctgaacgacg tggtaaaagc cgggaaagcg
    14581 cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg ctcaggcgct ggaactacaa
    14641 aaacagcact gctgggcgca gtttgtcagt atgcaggatc actataatct gatttatcgc
    14701 gaagaagagc gcgagatgct gcctctgtgt tatcaggagg gcgtggcggt gattccgtgg
    14761 agcccgatgg cgcgggggcg actgacgcgt ccatggggag aaactaccgc acgactggtg
    14821 tctgatgagg tggggaaaaa tctctataaa gaaagcgatg aaaatgacgc gcagatcgca
    14881 gagcggttaa cgggcgtcag tgaagaactc ggtgcaacac gagcacaagt tgcgctggcc
    14941 tggttgttaa gtaaaccggg cattgccgca ccgattatcg gaacttcgcg ggaagaacag
    15001 cttgatgagc tgctgaacgc ggtggatatc actttgaagc cagaacagat tgccgagctg
    15061 gaaacgccgt ataaaccgca tgcggtcgta ggatttaaat aagagtccag gcctgatgag
    15121 acgtgacaag cgtcacatca ggcatcggtg cacaactacg acagaatacc cagcggccag
    15181 tgatgaccga taaagtacag gatgcctgcg gaaatcaccc cggcgacaat atcgtcgatc
    15241 atgatcccca tgccgccatg tacattgcga tcaaaccagc ggatcggcca cggcttccac
    15301 atatccagaa tacggaaaat cacaaacccg gcggcaaccc actgccagtc attggtcggc
    15361 agcgccatga gcgtgatcca cataccaata aattcgtccc agacaatgct gccgtgatcg
    15421 tgcacgccca tatctttcgc cgtttgatga caaagataga cgccgataca gatccccaac
    15481 atcaccacca gcgagtagag ctgccagggt aaaaaggtca tcagatacca gaaagggatc
    15541 gccgccagcg atcccatcgt gccaggaacg attgggctta atccacttcc gaatccgaca
    15601 gcaagcagat gccacggatt actcatcttc aggcgacttt tcgcgacatc tttatggcgt
    15661 gacaaaatgg tcatatcctt tccagtcaaa cgtgacaggt tcgccgtcac gaataaaaca
    15721 aagcccttcg atatcggcgg tcatttgccc gatacaggta aacggtacgc caaggtgtcc
    15781 gagagcaaca tccagcgcgc cacggttcag ttccggcacc gtgaaacaca actcgtaatc
    15841 ttcaccgcca gagagcgccc agcgcagcgc ctgttccggt tcaacatggc gagaaagcgc
    15901 atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg cagtcgctgg ctttcacgat
    15961 atgcccgaga tcggaaatca gaccgtcaga gagatcgata gctgaatttg ccagatcgcg
    16021 cagtgcctgc ccctgtaaaa tacgcggcga tggacggaga tgacgtttga tcaagtagtc
    16081 agcatcttta gcatcggcaa cctgcaaacg gttttgcaaa atcgccagcc cggcggcgct
    16141 atcgcccggt gtaccggtca catagatcca gtcacccggt ttcgccccag agcgcgttaa
    16201 ggctcgtccc atcggaacaa agccgtggat acccaacgtc attgataatg gcccacgcgt
    16261 ggtatcgccg ccaatgagtt gcatatcgta ataattgaga agatcaaaca aactgtcgct
    16321 gaaggactca agccacgctt cgtctacgtc cggtaaggtt aatgccagcg tcagccaggc
    16381 cggatcggcc cccatcgctg ccagatcgct taggttcacc gccagtgctt tataagccag
    16441 atcagcagga tcgatatcag ggaggaaatg gttgcccgcc accagcgtat cagtgctgat
    16501 cgccagggtc tgtttctcgg ggatattgag aagagcgcag tcgtcgccga tgcccagttc
    16561 gacatcaaga cgagaacttc ttacacggtc aaaataacgg gcaatcaggg agaactcgcc
    16621 acatgccata cgttatgcct cagcaggtaa aaaagaaaag gccggcgatc atggaacagt
    16681 cttccgtgaa tctaccggcc tggatattac tttttgttag ggcgaatcac aggtgctgct
    16741 ttatcgagta cgccgttgac gaacttatgg ctgtcttctg cgccgaacga tttcgccagt
    16801 tcgatcgctt cgttaatggc cactttgtat ggcacatcac tacgtttaga cagttcgtac
    16861 agcgcaatgc gcagtactgc tttttctacc tggcccagtt cttccagcag gcgggacagg
    16921 tagggcttca tcagtccgtc gaggtatgcg gtgttagtcg ccaccccggc cagcagctca
    16981 cggaagtaca ggacgtcaac gtcttttaca tcctgttcag ccaggaactg gtattcaaca
    17041 tcagcgatgt cgttctggga caactgccag gagtagagcg cctggacggc acactcacga
    17101 gcgcggcgac gagcagcagg tttcacggat ttccccttac taatttcagg ccttgatggc
    17161 tttcaataca ttaatcattt caagcgcggt cagtgcagct tctgcacctt tgttgccagc
    17221 tttggtgcca gcacgttcga tcgcttgttc aatgctttca gtggtcagaa ccccaaaagc
    17281 aaccggaatt tcgctgtcct gggcaacatg cgccaggccg ttgcttgcac caccagcgac
    17341 atattcaaag tgggcagtgc caccacgaat aaccgtaccc agcgcaatca ccgcgtcgta
    17401 tttaccggtt ttagccagtg cacccgccgc cagcggcagc tcataggcac caggcaccca
    17461 aacaacggta atgttttcat cttttacctg accgatacgt ttcagtgcgt caattgcacc
    17521 ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg atggtgatgg cgacgcgagc
    17581 gtccggggta gcaacgttag cttcaataat gttcattttc ttccttcggg ttcgagtatg
    17641 gccccgcagg ggggcggatt ttagcataat atttcgtgcg ctgcttccct ttcgagccgg
    17701 gagagcatgc ccccactaaa tgcaggcaaa catccgggcc tacatgacgt atctctttga
    17761 atttaaattg gggggcgtcg gctaatttct caagccctgg cagcgagcat aatccacggg
    17821 cgtcgctgcc taatagttta ggtgcgatat agacaatcag ttcatcgact aaacccgcct
    17881 gtagcaatac gccagcgagc gttggccccg cttccaccca gatgctgtta atctgctgtt
    17941 tacccagttg catcatcagt acaaccagat ccagatgacc tttatgctct ggaatcagca
    18001 aggtacgcac cgtttccggc cactcacgag aatcttcctg cgtacgcgcg aaccaggttt
    18061 cgccgggctg ctgcacaatg cgatgttccg gcgtcacgcg attttggcta tcaatcacaa
    18121 tacgtactgg ctggcggaga ttttgttgcg ggtagagcgc ctgagtttgt tcatccagtt
    18181 cagaccaacg cactgttaag gctggatcat ccgccagcac cgtggcgctg ctggttaaaa
    18241 tggcatgact ttgtgcgcgc tgtcgttgta cgtcgcgccg cgcctgaggc gaagtgatcc
    18301 actggctttc gccgctcgcc atcgccgtgc ggccatcaag cgatgcgcca agttttaact
    18361 gaatataagg aaagccggtg cgcatccgct tgagaaagcc tttattcaat tgctcggctt
    18421 cactcatcat cagcccgtgg ctgacgtcaa tgccagcctg ttgcagacgg taaagtccac
    18481 gcccagcgac ctgcgggtta ggatcttgca tcgctgcgac cacgcgcgct acgccagcgg
    18541 cgatgagtgc gtcacagcac ggtggcgtac ggccatgatg gctacagggt tcgagtgtca
    18601 catacgccgt tgcgcctttg gctttttcac ccgccatacg caacgcgtgt acttcggcat
    18661 gtggttcacc cgctcgttgg tggtaacctt caccgacaat ttcgccatct ttgacaatga
    18721 cgcacccgac attcgggttg gggtgggtgg taaaacgtcc tcgttgcgcc agctttagcg
    18781 cccgcgccat gtaatactcg tcctgcacgg cttagtcctc caggcgcgcg atctcttcgc
    18841 caaattcttt gatatcttcg aaactgcggt agacagaggc aaaacggata taggcgactt
    18901 tatcgagctt tttcaattgc tccatcacca gattgccaat catcttgctc ggtacttcgc
    18961 gctcaccggt ggcgcgcagc tgcgatttaa tatgattgat tgccatttca acgtaatcgg
    19021 aactcaccgg acgtttttcc agcgcccgca gcattccgct acgcaatttc tcttcattaa
    19081 acggttcacg cacgtcgttg ctttttacaa cacgcggcat aaccagctcc gccacttcaa
    19141 aggtggtgaa acgttcatta cacaccagac actgccgacg gcggcgtacg gatgaaccct
    19201 cgcccacgag acgagagtca attaccttag tgtccacggc gaaacagaat gggcaatgca
    19261 tacggcgtcc tgaccaggtg gttaacggaa atctatttta ccctgaactg acgatacaac
    19321 aaaggcgcaa cgcttttgag ataaaggatc gatgctttcg ctgcttttac cctctaccat
    19381 taggctaatt gcgacgagaa agggacgacc tatgaatact aacgtttttc gactgctcct
    19441 gctgggaagc ctgttcagcc ttagcgcctg tgtgcagcaa agtgaagtgc gacagatgaa
    19501 acacagcgtc agcacgctga accaggagat gacgcaactc aatcaagaaa cagtcaaaat
    19561 cactcagcaa aacaggctga atgcaaaatc cagcagtggg gtttaccttc tgcctggagc
    19621 gaaaacaccg gcaagactgg aaagccagat cggtacttta cgtatgtcgc tggtgaatat
    19681 tacgcctgat acagatggca ccacccttac actacgtatt cagggtgagt cgaatgatcc
    19741 cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt cagggaacaa tagacaactt
    19801 tcaggaaatc aatgtgcaga atcaattgat taatgcccct gccagcgtcc tcgcccccag
    19861 cgatgttgat attccgttac aattaaaggg gatttctgtg gatcagttag gctttgtacg
    19921 tatccacgac attcaacctg ttatgcagta aacgtatttc gggacgattt tgtgcctccc
    19981 gcaacatctt tccccgtcat tttgttactc tgcttacatc acttggattg atagtaaaag
    20041 tttgcaacaa gggcgaaagt cagtacaatc cccgcccgaa tgtgtgtaaa cgtgaacgca
    20101 atcgattacg taaatgatag aactgtgaaa cgaaacatat ttttgtgagc aatgattttt
    20161 ataataggct cctctgtata cgaaatattt agaaacgcaa tttgcgcctt tttcactccc
    20221 gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac attactggca gccggtgcgg
    20281 tactggcgct ctcttcgtct tttactgtca acgcagctga aaacgacaaa ccgcagtatc
    20341 tttccgactg gtggcaccag agcgttaacg ttgtcggaag ctatcacacc cgtttcggac
    20401 cgcagatccg caacgatacc taccttgagt acgaagcatt cgctaaaaaa gactggttcg
    20461 acttctatgg ttatgcggat gcgccggtat tcttcggcgg taactccgat gctaaaggta
    20521 tctggaacca cggttctccg ctgtttatgg aaatcgaacc acgtttctcc atcgacaagc
    20581 tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg gtacttcgcg aacaactaca
    20641 tttacgacat gggtcgtaat aaagatggtc gccagagcac ctggtacatg ggtctgggta
    20701 ccgatatcga cactggcctg ccgatgagcc tgtccatgaa cgtctatgcg aaataccagt
    20761 ggcagaacta tggcgcagcg aacgaaaacg agtgggacgg ttaccgtttc aaaattaaat
    20821 actttgtgcc gattaccgat ctgtggggcg gtcagctgag ctacatcggc ttcaccaact
    20881 tcgactgggg ttccgattta ggggatgaca gcggtaacgc aatcaacggt attaagatcc
    20941 gtactaataa ctctatcgct tccagccata ttctggctct gaactacgat cactggcact
    21001 actctgtcgt agctcgttac tggcacgacg gtggtcagtg gaacgacgat gcagaactga
    21061 acttcggcaa cggcaacttc aacgttcgct ctaccggctg gggtggttac ctggtagtag
    21121 gttacaactt ctgattatga aaatgccggg atttattccc ggcatttctg attgttaaca
    21181 acgtgtgctt tgttcatgcc ggatgcggcg taaacgcttt gtccggccca caaaagcacg
    21241 taaattcaat atattgctga aataacgtag gcctgataag cgtagcgcat caggcaattt
    21301 tacgttttaa tcacttcttc ttgttcatca tcgctttcag gtcagcgaat gggttgtact
    21361 tcgcttcacc gacatctttc tgcgcatctt cccctgcgat aacggtcgta ccatactgat
    21421 ccgcttcggt atatttcgaa tgctcatgat cgtggcaata gagacacaac aattcccagt
    21481 tactgccatc ttccgggtta ttggtatggt cgtgatcaat gtggtgaacg gtaagttcac
    21541 gcaggttaga ataaacaaac tcgcgggaac agcgaccgca gacccacgga tagattttta
    21601 atgctttttc gcgatagccg ctttctaacc gtgcatagtt ttttgggatg atagccatga
    21661 aaagttacct ggatgaaatg tagggttacg cgcagtttcc caagatgcgg ggattttcgc
    21721 aatctccata catcaaaaaa aatcccgatc ttctgaccgg gattttcaac atcaacggga
    21781 acttgattaa ggcagaattg acggctgatc cgccccttct ttttccactt tctgctgcaa
    21841 catgtgttcg cgcttcatac ccagtttcag agccaacgca gatgctacgt agatggaaga
    21901 tgcagtgccg atggaaacac cgataagcat ggtcagcgag aagccttcca gtaccggacc
    21961 accgaagaga tacagcatca ggataaccat caaggtagta ccggatgtga tcaaggtacg
    22021 gtgcagcgtc tgggtcaagg acacgttaaa gatttcgtaa ggcgtaccgc gacggatctt
    22081 gcggaagttt tcacgaattc ggtccgatac cacgatactg tcgttaagcg agtaaccgat
    22141 aaccgacatc aacgatgcca caatggtcag gtcaatctcg atatggaata acgacaaaat
    22201 acccagcgta atgataacgt cgtgcgccag cgcaataacc acccctgccg ccagtcgcca
    22261 ctcaaagcgg aaacctacgt acacgaggat agacagcagc gctgccatca acgccatcgc
    22321 accggtttgc gcaaggtctg cccccacgct cggaccgacg aactcaatac gcttcactgc
    22381 tgcattctga ttggtggatt cgttaatcac cttcagaact tggctgccca gcacctgacc
    22441 gccggtttcg ccttcagcag gcggcatacg gaccatgatg tcgtggctgc taccaaagtt
    22501 ttgcagcatt ggctcttcaa aaccggcttt ttgcaatgca tcacgcatta cgtcaatttc
    22561 agccggtttt tcgagcgtaa tttcgataac cgtaccaccg gtgaaatcca gcccccagtt
    22621 aaagccgcgc acgcccataa taacgatagc agcgattaac agcagaccag agatgccgaa
    22681 agcccagtag tcccagcgca taaagtcata gactttacgg ccgtggttta gttgttcaac
    22741 agtatattcc tgtgccacat cgcactcctc agattgacag cttcttgacg cgcttgccgc
    22801 catatagcag gtttacgatg gcacgcgtac cgacaatcgc ggtaaacatc gacgtcgcca
    22861 caccgatacc ggtagtaatc gcgaaccctt taattgcccc ggtacccact gcgtacagga
    22921 tgatgacttt aatcagcgtg gtgatgttcg catcgaagat agaactgaat gcgccacgat
    22981 aaccttcatc aattgcctgt tgaacagtac gtccgttgct caactcttct ttaatacgtt
    23041 cgttgatcag tacgttcgca tcgaccgcta ccgcaagggt taagacgata cccgcgatac
    23101 ctggcatact cagtgttgcg cctggcaaca gcgacataat accgacgatc aagatcaagt
    23161 tggcaatcag agcactggtc gcaatcagac caaacttctt atagaagatg atcatgaaca
    23221 gaatagaaac cagcagaccg gcaaggcaag cctccagccc ctgctcaatg ttctgcatac
    23281 ccagggtcgg gccaattgta cgttcttcaa caatctgaat cggcgcgatc aacgcaccgg
    23341 cacgcagcag cagtgacagc tgacgggctt cgttcgggtt gttgatgccg gtgatacgga
    23401 agctgttacc cagacgagac tggatgttgg cgatgttaat cacctcttcc tgtttcacca
    23461 gaaccgcacg accatttgca tctttcttac cgctgtcttt gtactccaca aacagggttg
    23521 ccatcggttt gccgatgttg tccttggtga agttagacat aatgttgcca ccagcgctat
    23581 cgagcgagat gttaacctgc ggctggttgt attcgtcctg gctggaagtg gagtcggtga
    23641 tatggtcacc agtcagaatt acgcgtttgt acagcacaac cggctgacct tcgcgggtct
    23701 gtttcacttc agagtcgccc ggtacgcgac cggatgccgc agcggcctga tcaacattgg
    23761 tgtttaccag acggaattcc agcgttgccg tcgcgcccag aatctctttc gcacgcgcag
    23821 tgtcctgaat accaggcagt tcaacaacga tacggtcagc accctgacgc tgaaccaccg
    23881 gctccgccac gccaagttgg tttacacggt tacgcaggat attaatgttc tgctgcaccg
    23941 catattcacg cgcttcactc agacgagcat cgctcattac cgcacgcagc tggttgctgc
    24001 cctggctgct aatcaccagg tccggatggc gcttgctcag atacgcaatg gcttcatcac
    24061 gagctttagc atcgcggaaa gtgatgctca ggccgtagtt gttttcttta cgaacagtgg
    24121 tatacgggat gcccttttcg cgcaggtcac tgcgtaggct atcgatattt tgttcctgga
    24181 gtttgccaag cgcggtgtcc atatccactt ccatcaggaa gtgaacgccg ccacgcaggt
    24241 caaggccgag cttcatcggc tcagcatgaa tagctgccag ccagcgcggc gttgccgggg
    24301 caaggttaag cgccacgacg tatttgtcac ccataacgcc cattaatgct tcacgagcgc
    24361 gcaactgggt gtcagtggag tcgaagcgcg caagaatagc gccctcttcc agtgccacag
    24421 acttagcagt tattttttct tcttgtaacg ttttctggac ctggatcagc gtttgctcac
    24481 tggcggcgac tccgcgcgca ccagtgatct gaacagccgg atcctcacca aacaggttgg
    24541 gaagcgcata cagcagaccg atgacaatca ccacgatcag catgacgtac ttccacaaag
    24601 gataacggtt taacacggca attcccttag ggaaaaattt taattacagc gccttcatgg
    24661 tgcctttcgg caggacggca gctacgaagt cacgtttaat aactacttca gtggtgtcat
    24721 tcagcgcgat agcaatgtag ccgttttccg ctactttggt tacgcgacca accaggccac
    24781 cgttcgtcag aacttcatca cctttggcaa tggagtccat cagctttttg tgttctttgg
    24841 tgcgcttctg ctgtggacgc aggatcatga aatagaaaat cagaccgaat accaccagca
    24901 tcaaaatcaa agacatcggg ctaccttgcg ccggtgcacc cgttgccgct accgcatcag
    24961 aaataaaaaa gctcattaaa tttccctcat tattaatatt aatcaacgtt caaaggtggt
    25021 acttctcgcc cctgacgctg gtaaaaatca gttacgaagc tctctaattt accctcttca
    25081 atagccttgc gtaaacccgc catcaaacgc tggtagtaac gaaggttatg aatggtgttg
    25141 agtcgcgcgc ctaatatttc gttgcaacgg tcgagatgat gcaagtaagc gcgtgaataa
    25201 ttgcgacagg tgtagcaatc acactcagga tcgagtgggc cagtatcgct cttatatttc
    25261 gcattgcgga ttttcaccac gccatcggtc acgaacaaat gaccatttcg ggcgttgcgg
    25321 gttggcatta cgcagtcaaa catatcgata ccgcgacgta cgccttcaac caggtcttcc
    25381 ggtttaccaa cgcccatcag gtaacgcggt ttgtctgccg gaatttgcgg acacacatgc
    25441 tccagaatac ggtgcatatc tgctttcggc tcacccacag ccagaccgcc gacagcgtag
    25501 ccatcaaagc cgatatctac cagaccttta acagatatat cacgtaaatc ttcgtaaata
    25561 ctgccctgaa ttataccaaa taacgcattt ttgttaccaa gactgtcaaa acgctcacgg
    25621 ctacgcttcg cccaacgcag agacatctcc atagagcgtt ttgcgtaatc ccagtcagca
    25681 ggatacggcg tacactcatc aaagatcatg acgatatccg aaccaagatc gtactgaatc
    25741 tccatcgact tttccgggtc gaggaaaatc gggtcgccgt tgatcgggtt acggaagtga
    25801 acgccctgtt cggtgatttt acgaatatca ccaaggctga agacctggaa gccgccggag
    25861 tcggtaagaa tcggtccttt ccactgcata aaatcgtgca gatcgccgtg cagtttcatg
    25921 atttcctggc ccgggcgcag ccacaggtgg aaagtgttgc cgaggataat ttgcgcgcca
    25981 gtggcttcaa cttcttccgg cgtcatccct tttacggtgc cgtaggtgcc aacaggcata
    26041 aaacaaggcg tttccactac gccacgatca aagaccaggc ggccacggcg tgcgcgaccg
    26101 tcggtggtgt ccagttcaaa tttcattttt tctccactac gtcagaaaaa cagtccaacg
    26161 tttaaaccag cgccgcggaa ttactccccg acgcgctcat taattgcctg cggattgtac
    26221 gtgataaaca tcgcatcacc gtaactaaaa aagcgatatt tctcttctac cgccgcttta
    26281 taggcattca tggtgtgttg ataaccggca aaggccgaaa ccagcataat cagcgtcgac
    26341 tctggcaagt ggaagttcgt caccagcgca tcgaccactt tgtactggaa gcccggatag
    26401 ataaagattt gggtatcgtc aaagaacggt tcgataagat cgttttttgc cgcctgtgcc
    26461 gcgctttcca gcgaacgtac tgaagtggtg ccaaccgcaa tcacccggtt accgcgcgct
    26521 ttcgccgcca gtaccgcgtc taccacatcc tgcggtactt cagcgtattc cgagtgcatg
    26581 atgtgatctt caatggtgtc gacgcgcacc ggctggaagg tgcccgcacc aacgtgcaac
    26641 gtcacaaacg ccatctccac gcctttggcg cgtaattttt ccagcaaagg ctcgtcaaaa
    26701 tgcagaccag cggtcggggc tgcaaccgcg cccggttttt cgctataaac ggtttgataa
    26761 agttcgcggt cagcgtcttc gtccggacga tcgatatacg gcggcagcgg catatggccg
    26821 atgctgttga ggatatccag taccgagcgt tcatcattaa attcgacttc aaacagtgcg
    26881 ccgtggcgcg cggtcattgt tgcgttgata ctttcgtcat cgcccagcaa cagttctgcg
    26941 ccaggttttg gcgctttcga ggcgcgaata tgcgcaagaa tgcgtttgtc gtcgagcatc
    27001 cgctcaacca gcacttcaat cttgccgccg ctggctttac gcccaaacag acgcgccggg
    27061 atcacgcggg tattattaaa aaccagaaga tcgccggggt tgagcttatc gagtaaatcg
    27121 gtgaaagtac cgtgcgtcag cgcacccgtc ggcccgtcca gcgacagtaa acgacagcta
    27181 ctgcgttcag gcatgggata gtgggcaatc agggattcgg gcaattcaaa ggagaaatcg
    27241 gtaacgcgca tgacactgac tcagactaaa ataagagtcg ggtagtctag tgccggggcg
    27301 ctctccctgc aacctttacc cccctttgga tagaatataa tcgatgaatt ttttagctca
    27361 cctgcattta gcccatctcg cggaaagctc gctttctggc aatttactgg ctgatttcgt
    27421 acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg gctggcattc atatgcatcg
    27481 acgtattgac tgacaatctg ccggaagtcc gcgaagcacg ggagtggttt cgtagtgaaa
    27541 cgcgccgcgt tgcgcctatt acgctggatg tcatgtggga tcactttctt tcccgccact
    27601 ggtcgcagct gtcgccggac tttccgctac aggaatttgt ctgttatgcc cgcgagcaag
    27661 tgatgacgat tttgccggac tcaccgccac gttttatcaa tctgaacaat tacttgtggt
    27721 cagagcagtg gctggtgcgc tatcgcgata tggatttcat ccagaacgtg ttaaacggca
    27781 tggcaagccg ccgcccacgt ctggatgccc tgcgtgactc ctggtacgat ttagacgctc
    27841 attatgacgc cctcgaaccc cgcttctggc agttttatcc gcggatgatg gcgcaggcgt
    27901 cacacaaggc gttataaaac gttagttcat ccataccgta gccgaaatgg caggcaaagc
    27961 cagaatcccg tcagtcagtt gcccatgccc ttctttgcat tgccattgca cggcattgag
    28021 aaacggtgac gcgggtagca ccacttcact ggcctcgcca cggttgattg ccaccagtac
    28081 acgttgctga ttcagcacgc ggacaaatac cactacgtta tcttccgcat acagcacctg
    28141 acagccgcca cgacgcagcg cctgactttt cttacgcagc acaatcattc gccggtacag
    28201 cgcgaataac gccgtatcct gcttttccac ctgccagggg aacggtttac ggcaagacgg
    28261 atcgttttta ccatccagtc ccacttcatc accgtaataa atgcacggta caccaggcca
    28321 ggtgaacagc cagaccaccg ccagcggcag gcgcgcaata tcccgaccga gcagcgtttt
    28381 aaatcgcgca gtatcgtggc tatcgagctg attaaacata cgtaattgtt gttgatgaga
    28441 aagccctgcg cggtaattat ccatccaggc catacaggtt tgggcgtcaa tttgctgcgg
    28501 atcataagag atgtcggtat tggcaagaaa tccccacaac gggaatgtga agccacgata
    28561 gttcatggca gcatcttcca catcggcctg taaccattgc cgtgcatcgc caaaatgttc
    28621 gccgacaata tacgcttccg actgggtttc tttcgccgct tcggtgatcc cggcaacgtg
    28681 ctgcatatta ttgcgcgccc cacccgcctc ccccagcata tgcaccacat ccagccgcca
    28741 gccgtccata ttccacggcg ctttcagcca gtggcggaca atactgtctt ccccgcgata
    28801 aatttcattc accagacttt ccgactgata atccagcttc ggcaagctgg catagccaag
    28861 ccagtcgagc gccgtgccat catcactaaa caagtaccag tcgcgccagg gcgattcaga
    28921 gttgtgacaa gctccccccg tgccaagatt gtgcctgtca aaccaggcat gggaatcgcc
    28981 actgtggtta aacacgccgt ccagcaccag ccgcattccc agctgctgcg tattgtgtcg
    29041 caaacgcagc aacgccccat caccgccaaa ctgcggatcg acatggcgat aatcctcggt
    29101 atcgtattta tgtacgctgg gagctttaaa caccggattg agatacagcg ctgtcacgcc
    29161 aagctttttc agatacggca gtttttcgct tatcccgtcc agatcgccgc catagaacgt
    29221 tgatccgccc gcctgcgccg tgaccggttc atcccagtca cgcaagatga tctcttgtcc
    29281 ggctgcatga tggtaataga catgatcctg ttcagcttca cgaggaagac tacgcgcaaa
    29341 acgatcaggg aagatctgat aaaaaatctg atccgcagcc cattgtgggc cgatatccgg
    29401 tacatcgacg gcaaactgct ccagtcgtgc cggcggcatt cggctgaagc cctgcggtgt
    29461 aaaccaacgc tggcgatcgt gccacagcag tttgaaactg taacgccgcc ggggttgtcc
    29521 gctggagaga tcaatcgccg cacgccatgc ggtgacgcct ggctgcggct gactgcgctg
    29581 cttatgcatt ggtactgaca tttcttcgtt atcgtgttct gtacgcagca taatgcgctg
    29641 cggtgggtct tcgcccgtca gccacagtgt aatgagcagt tgatctttgc tttgtttaac
    29701 aaatgggggc accggcaggt gccatgcatt taacatcata attcccctgt gatgaaaatc
    29761 tgatcacctt gccatagcct aatgcaggat cactcatcat ctgaaggata tttgggggag
    29821 gaggacgggg gcggataact ttcggaatag ccggatcagg atttcctcct gaaccggctt
    29881 acagaacgca agtattattg gttttcagcc agctgacgat cgtggcggcg tttaaacatc
    29941 cagccaatca acagcacaac aatccacgcg aaaccgacat acagcgagat acgcgtatcc
    30001 gggtgataac caatcaaccc gataataaag agcaggaaaa tcagcccgcc gatggtcgtt
    30061 gctaccccac ccggcacttt aaatttcagc gccttaactt cttctggcgg caaacggcga
    30121 cggaaggcga tttgcgacag caggatcata atccacaccc ataccgtggc gaaggttgcc
    30181 agcgaagcga tcaccaggaa gacgttttcc ggcatgatgt agttcagata caccgcaaac
    30241 agcagcgcgg tagtcatcac cagcaccgta acccacggaa taccgcgacg cgacgttttg
    30301 ctgaaaattt tcggtgcgct gccctgctct gccataccgt ggagcatacg gcctacgcca
    30361 aatacgtcac tgttaattgc cgacagcgaa gcggtcagca caacaaagtt aagaatgctg
    30421 gcggcaaagg taatgcccat atgctggaac gtcagcacga acgggctacc ggcagtgcca
    30481 acctgattcc acgggtagat agacataatg acgaacagcg taccgacgta gaacaccaga
    30541 atacgcatcg gcacggagtt aatcgcacgc ggaatcgatt tctcaggatc tttcgcttca
    30601 ccggcggtaa tcccgataat ttcgatccca ccgtaagcaa acatcaccat ttgcaacgac
    30661 attaccatgc caagccagcc gttactgaag aagccgccgt tgctccacag gttatgaata
    30721 ccggtcggtt gcccgccgtt gccaatcccc cagatgatga tgccgaaacc ggcgacaatc
    30781 atgatgatga tggtggcgac tttaaagaac gagaaccaga attccagctc accgaagacc
    30841 ttcacgctca tcaggtttac ggcgcaaatg atcagcacca cgctcagtac ccaaatccag
    30901 tgcggcaccg tcgggaacaa gacacccata tagataccaa aagcggtcac atcggcgatg
    30961 gcgacaataa ggatttcaaa gcagtaggtc catccggtaa tgtaacctgc cagcgggcca
    31021 aggttttcct gcgcataacg cgagaaagag ctggcggccg ggttatgtac cgacatttcc
    31081 cccagcgcac gcataatgat atacgccgcg ataccaccga taatataggc caacaacacg
    31141 ctcggaccgg ctattttgat ggcgtctgcc gaaccgtaaa acagcccggt gccaattgct
    31201 gaacccagtg ccataaagcg tatgtggcgg gtacttagcc cacgctttag cttattctta
    31261 ctttccatca attcctaacc catcaacaca ataaaaaacc acggggcatc gcagccccgt
    31321 ggttaaaaca aatgttcagt gatttagtga gcgctggagg tcacctgacg acctgccgcg
    31381 cgatcccaga taatggccag aaccaccatc accactgttg gcattaacca cgccagacct
    31441 tgttcggcca gcggtaaacg ctgcgcccag gacggtaaga tatcgctgaa tgcagatgct
    31501 ttgatcccgt cgagaatacc aaaaagcagg ctgataaaca tcggcggagc aatcacgcgg
    31561 gacgaattat gccaccatga gcgtgtaaaa cttaatacaa ccagtgcgat acacggcgga
    31621 taaatagcgg tcagcaccgg tacggagatc tggatcagct ggcttaagcc gaggttagac
    31681 accaccatcg agaagccgcc gaggataaac accagcgtac gataagagag cggtacgtac
    31741 tgggcaaaga attctgcaca agcacaggtc aggccaaccg ccgtgaccag acaggcgatg
    31801 aagattaacg ccgccagcag gaagctaccg ccgccgccaa aggtgtgctg aacgtaagca
    31861 tgcagaatag cagcgccgtt tgcagactga tcgaccagcg acgcgctgtc tgaacccaga
    31921 cagaacagcg ccaggtacag caaagtcaga ccaacacccg ccatcaggcc agcccagacg
    31981 gtataacggg tcagcagacg cgcttcggta acgccacgag aacgcgccgc gttaacaata
    32041 acgataccaa acaccattgc gcccagcgta tccatggtca gatagccgtt aacgaagccg
    32101 ttagaaaatg cagcgttttg ataagcctca gtcgccgtgc tgatagaacc cgccggccag
    32161 acaatcgcga caacagacag gatgaccagc gcgataattt tcagcggcgc aaggaagttg
    32221 cccacggtat ccagcagctt gcccggatag agcgaaacca gaataacgat agcgaaatag
    32281 accaggctgt agataaacag cggcagcgcg gaatcacccg tcagcggcgc aatccccact
    32341 tcaaaggaaa cggtagctgt acgcggcgta gcgaaaagcg gccccaccgc caggtaacaa
    32401 accgttgcca gcagtacgcc agcgacttta ccgattggcg tgctgaggct gtcaacaccg
    32461 ccgccaactt ttgccagcgc cactaccgtt aataccggca ggccaacggc agtaatgagg
    32521 aagccgaatg ccgcagtcca gacgtgttcg cctgcctgta agccgaccat tggagggaaa
    32581 ataatgttac ctgcgccgac gaacaacgca aatgtcataa agcccagagc gatgatatcg
    32641 cgcgatctta attgatgggt cataaatcat actgcctgtg gatgtggtgt tgtaacgtta
    32701 aatttttgtg ccaatccctg gggatgatac agcggtatat ttaaacaatt ttagcgggaa
    32761 atactcccaa ccaggcgcgg cgaagaatag tttttcgatt taccgcgtta atgcagtcag
    32821 tatgacagca cctgaagcgc aatttaaacg cttataacgt tttaaagcaa ggagggatct
    32881 aaaaaccaga ggtatatagc gataagaaat caccaaccag tataaaacac tatttccgtg
    32941 aaaaagacat ggctaatcat gcgaacaaaa aatcaccaac cgttgtaaaa tctaacgccc
    33001 gtattttgac tgcaaagaaa ataagccagc atcggctggc ttatggcaat aaaagatgac
    33061 aaaggcggat taatcgctgt ttttggcaat taaacgttcc gggataacaa aactgaaacg
    33121 tgttcctttt cctactgtac tctcaatatt caggcgactt tcgtgatgat tcaccgcatg
    33181 tttcacgatc gctaacccta atccgctacc gccggtttgc cgggaacgcg ctttatcaac
    33241 gcgataaaaa cgctcggtca ggcgcggaat atgctccggt gcaatgcccg gtccgttatc
    33301 ttcaacgcta aattcggcac cgtgcggcac tcgctgccag cgtacggtga tatgcgtgcc
    33361 ttccggcgta tgattcacgg cgttatagac caggttcgaa atcgcactgc gtagctgatc
    33421 ttcgttgcca gacaccttga ggccgttatc tatctcaaag gtaaatgtct gttttttctg
    33481 actcagagtc tgagcctcgc gctcaacaac gcgcagcatc atcggcacat caaccttttc
    33541 attgagcaat tgcgtcggcg aggcttcaat tttcgacagc gttagcaact gcttcaccag
    33601 cccttccatc cgctgggtct gctcgcgcat ggtgtgcaac gctttttcgc gtaccgcgcc
    33661 ttccagcggc tgctcatcca tcatctccag gtaaccctgt aacacggtca atggcgtacg
    33721 taactcatgg ctcacgttgg caaaaaagtt acgccgcgcc ccttccagtt gatgcatttg
    33781 cgtgacatca cgcgccacca tcaacaactg tttgtgggta taaggcatga cgcgaatttc
    33841 cagatgccgc ccggtgttga gcaccagatt gagcgggcga gaaaaatcac gcgttttcag
    33901 atattgcgta aactccgggt aacgcagtag gttaaggatg ttctgcccgt tatcttccgg
    33961 ccagcgcaaa ccaagaattt gttgcgccag accgttacac cagaaaatac cgccctcttc
    34021 cgtggtcagc accaccgcgt cgggcagcga ctccgcgccg ctacgaaagc gtttaatcag
    34081 attgcccagt tcacggcggc gttttttatt tcgcagctgc atctggtgta agccgtatag
    34141 tagcggttcc cagctaccac gccccggtgg cggggtcata ctgcgatcca cccacagcca
    34201 ccatgaaagg cgcaataaat tccagaaatg ccagataagc agtcctgtta ccgatgccag
    34261 caaaaaccag ggcaggtaac caaaaaatgc acccaggatg aaagccggga ggcagcaaag
    34321 tagcagctcc agcaccagcc ttttccacga cagccgttcc agcacgtaag atactccagt
    34381 taagaaatca taagccctgc tctgcgtccg atgagcaagg cgttaaaagc gggttgaaaa
    34441 acgatatcct gtaccgcgca cggtctgcac catgcggtca tgcccgccgg gctccagtgc
    34501 tttacgcagg cgacgaatgt ggacatcgac cgtgcggtct tccacataca cgttagttcc
    34561 ccagacgtgg tttaacagct gctcgcggct gtacacgcgc tcaggatgcg tcataaaaaa
    34621 gtgcagcagt ttaaattctg tcggccccat ctccagcggc tcttcgcccg ccatcactcg
    34681 gtgagatgtc gggtcgagac ttaatccctg catctcaatc acctcttcca ccgccattgg
    34741 cgaaatacgg cgcattaccg ctttgattcg cgccaccagc tccttcggcg aaaatggctt
    34801 ggtgatatag tcatccgcgc cggtttcaag gccgcgcacg cgatcttctt cttcccctct
    34861 ggcggtcaac atcaccactg gaatatcccg ggtcatcgac tcgcgcttga ggtgtttgat
    34921 gaactggata ccggagccgc caagtaacat ccagtcaagg agaattaaat ccggccaagg
    34981 ttcattcagt tgattcacag cactgtcata atcttccgct tcgaccggct gaaagccatt
    35041 ttgttcgagc acgaagcaga ccatttcgcg aattggagct tcatcttcta cgaccagaat
    35101 acgtctcgcc atgatttgcc ctgttgtaat aaataggttg cgatcattaa tgcgacgtca
    35161 ttatgcgtca gatttatgac agatttatga aaagctcgtc gcacatatct tcaggttatt
    35221 gatttccgtg gcgcagaaaa aagcaaatgg cacatctgtt tgggtataat cgcgaccatg
    35281 ctttttcgcc agggaaccgt tatgcgcatc cttcacacct cagactggca tctcggccag
    35341 aacttctaca gtaaaagccg cgaagctgaa catcaggctt ttcttgactg gctgctggag
    35401 acagcacaaa cccatcaggt ggatgcgatt attgttgctg gtgatgtttt cgataccggc
    35461 tcgccgccca gttacgcccg cacgttatac aaccgttttg ttgtcaattt acagcaaact
    35521 ggctgccatc tggtggtact ggcaggaaac catgactcgg tcgccacgct gaatgaatcg
    35581 cgcgatatca tggcgttcct caatacaacc gtggtcgcca gcgccggaca tgcgccgcaa
    35641 atcttgcctc gtcgcgacgg gacgccaggc gcagtgctgt gccccattcc gtttttacgc
    35701 ccgcgtgaca ttatttccag ccaggcgggg cttaacggta ttgaaaaaca gcagcattta
    35761 ctggcagcga ttaccgatta ttaccaacaa cactatgccg atgcctgcaa actgcgcggc
    35821 gatcagcctc tgcccatcat cgccacggga catttaacga ccgtgggtgc cagtaaaagt
    35881 gacgccgtgc gtgacattta tattggcacg ctggacgcgt ttccggcaca aaactttcca
    35941 ccagccgact acatcgcgct cgggcatatt caccgcgcac agattattgg cggcatggaa
    36001 catgttcgct attgcggttc ccccattcca ctgagttttg atgaatgcgg taagagtaaa
    36061 tatgtccatc tggtgacatt ttcaaacggc aaattagaga gcgtggaaaa cctgaacgta
    36121 ccggtaactc aacccatggc ggtgctgaaa ggcgatctgg cgtcgattac cgcacagctg
    36181 gaacagtggc gcgatgtatc gcaggagcca cctgtctggc tggatatcga aatcactact
    36241 gatgagtatc tgcatgatat tcagcgcaaa atccaggcat taaccgaatc attgcctgtc
    36301 gaagtattgc tggtacgtcg gagtcgtgaa cagcgcgagc gtgtgttagc cagccaacag
    36361 cgtgaaaccc tcagcgaact cagcgtcgaa gaggtgttca atcgccgtct ggcactggaa
    36421 gaactggatg aatcgcagca gcaacgtctg cagcatcttt tcaccacgac gttgcatacc
    36481 ctcgccggag aacacgaagc atgaaaattc tcagcctgcg cctgaaaaac ctgaactcat
    36541 taaaaggcga atggaagatt gatttcaccc gcgagccgtt cgccagcaac gggctgtttg
    36601 ccattaccgg cccaaccggt gcggggaaaa ccaccctgct ggacgccatt tgtctggcgc
    36661 tgtatcacga aactccgcgt ctctctaacg tttcacaatc gcaaaatgat ctcatgaccc
    36721 gcgataccgc tgaatgtctg gcggaggtgg agtttgaagt gaaaggtgaa gcgtatcgcg
    36781 cgttctggag ccagaatcgg gcgcgtaacc agccggacgg taatttgcag gtgcctcgcg
    36841 tagagctggc gcgctgcgcc gacggcaaaa tcctcgccga caaagtgaaa gataagctgg
    36901 aactgacagc gacgttaacc gggctggatt acgggcgctt cacccgttcg atgctgcttt
    36961 cgcaggggca atttgctgcc ttcctgaatg ccaaacccaa agaacgcgcg gaattgctcg
    37021 aggagttaac cggcactgaa atctacgggc aaatctcggc gatggttttt gagcagcaca
    37081 aatcggcccg cactgagctg gagaagctgc aagcgcaggc cagcggcgtc acgttgctca
    37141 cgccggaaca agtgcaatcg ctgacagcga gtttgcaggt acttactgac gaagaaaaac
    37201 agttaattac cgcgcagcag caagaacaac aatcgctaaa ctggttaacg cgtcaggacg
    37261 aattgcagca agaagccagc cgccgtcagc aggccttgca acaggcgtta gccgaagaag
    37321 aaaaagcgca acctcaactg gcggcgctta gtctggcaca accggcacga aatcttcgtc
    37381 cacactggga acgcatcgca gaacacagcg cggcgctggc gcatattcgc cagcagattg
    37441 aagaagtaaa tactcgctta cagagcacaa tggcgcttcg cgcgagcatt cgccaccacg
    37501 cggcgaagca gtcagcagaa ttacagcagc agcaacaaag cctgaatacc tggttacagg
    37561 aacacgaccg cttccgtcag tggaacaacg aactggcggg ttggcgtgcg cagttctccc
    37621 aacaaaccag cgatcgcgag catctgcggc aatggcagca acagttaacc catgctgagc
    37681 aaaaacttaa tgcgcttgcg gcgatcacgt tgacgttaac cgccgatgaa gttgctaccg
    37741 ccctggcgca acatgccgag caacgcccac tgcgtcagcg cctggtcgcg ctgcatgggc
    37801 agattgttcc ccaacaaaaa cgtctggcgc agttaatggt cactatccag aatgtcactc
    37861 tggaacagac gcaacgtaat gtcgcactta acgaaatgcg ccagcgttat aaagaaaaga
    37921 cgcagcaact tgccgatatg aaaaccattt gcgagcagga agcgcgcatc aaaacgctgg
    37981 aagcccagcg tgcacagtta caggcgggtc agccttgtcc actttgtggt tccaccagcc
    38041 acccggcggt cgaggcgtat caggcgctgg agcctggtgt taatcagtct cgattactgg
    38101 cgctggaaaa cgaagttaaa aagctcggcg aagaaggtgc ggcgctgcgc gggcaactgg
    38161 atgcattaac gaagcagctt cagcgcgatg aaaacgaagc gcaaagcctc cgacaagatg
    38221 agcaagcact tactcaacaa tggcaagccg tcacggccag cctcaatatc accttgcagc
    38281 cacaggacga tattcaaccg tggctggatg cacaagatga gcacgaacgc cagctgcggt
    38341 tactcagcca acgacatgaa ttacaagggc agattgccgc gcataatcag caaattatcc
    38401 agtatcaaca gcaaattgaa caacgtcagc agcagctctt aacggcattg gcgggttatg
    38461 cactgacatt gccacaggaa gatgaagaag agagctggtt ggcgacacgt cagcaagaag
    38521 cgcagagctg gcagcaacgc cagaacgaat taaccgcgct gcaaaaccgt attcagcagc
    38581 tgacgccgat tctggaaacg ttgccgcaaa gtgatgatct cccgcacagc gaagaaactg
    38641 tggagctgga taactggcgg caggttcatg aacaatgtct cgcattacac agccagcagc
    38701 agacgttaca gcaacaggat gttctggcgg cgcaaagtct gcaaaaagcc caagcgcagt
    38761 ttgacaccac gctacaggcc agcgtctttg acgatcagca ggcgttcctt gcggcgctaa
    38821 tggatgaaca aacactaacg cagctggaac agctcaagca gaatctggaa aaccagcgcc
    38881 gtcaggcgca aacgctggtc actcagacag cagaaacgct ggcacagcat caacaacacc
    38941 gacctgacgg gttggctctc actgtgacgg tggagcagat tcagcaagag ttagcgcaaa
    39001 ctcaccaaaa gttgcgtgaa aacaccacga gtcaaggcga gattcgccag cagctgaagc
    39061 aggatgcaga taaccgtcag caacaacaaa ccttaatgca gcaaattgct caaatgacgc
    39121 agcaggttga ggactgggga tatctgaatt cgctaatagg ttccaaagag ggcgataaat
    39181 tccgcaagtt tgcccagggg ctgacgctgg ataatttagt ccatctcgct aatcagcaac
    39241 ttacccggct gcacgggcgc tatctgttac agcgcaaagc cagcgaggcg ctggaagtcg
    39301 aggttgttga tacctggcag gcagatgcgg tacgcgatac ccgtaccctt tccggcggcg
    39361 aaagtttcct cgttagtctg gcgctggcgc tggcgctttc ggatctggtc agccataaaa
    39421 cacgtattga ctcgctgttc cttgatgaag gttttggcac gctggatagc gaaacgctgg
    39481 ataccgccct tgatgcgctg gatgccctga acgccagtgg caaaaccatc ggtgtgatta
    39541 gccacgtaga agcgatgaaa gagcgtattc cggtgcagat caaagtgaaa aagatcaacg
    39601 gcctgggcta cagcaaactg gaaagtacgt ttgcagtgaa ataactattc agcaggataa
    39661 tgaatacaga ggggcgaatt atctcttggc cttgctggtc gttatcctgc aagctatcac
    39721 tttattggct acggtgattg gtagccgttc tggtggttgt gatggtggta tgaaaaaagt
    39781 cattttatct ttggctctgg gcacgtttgg tttggggata gccgaatttg gcattatgag
    39841 cgtgctcacg gagctggcgc ataacgtagg aatttcgatt cctgctgccg ggcatatgat
    39901 ctcgtattat gcactggtgg tggtggtcgg tgcgccaatc atcgcactct tttccagccg
    39961 ctactcactc aaacatatct tgttgtttct ggtggcgttg tgcgtcattg gcaacgccat
    40021 gttcacgctc tcttcgtctt acctgatgct cgccattggt cggctggtat ccggctttcc
    40081 gcatggcgca ttttttggcg tcggagcgat cgtgttatca aaaattatca aacccggaaa
    40141 agtcaccgcc gccgtggcgg ggatggtttc cgggatgaca gtcgccaatt tgctgggcat
    40201 tccgctggga acgtatttaa gtcaggaatg ctggcgttac acctttttat tgatcgctgt
    40261 ttttaatatt gcggtgatgg catcggtcta tttttgggtg ccagatattc gcgacgaggc
    40321 gaaaggaaat ctgcgcgaac aatttcactt tttgcgcagc ccggccccgt ggttaatttt
    40381 cgccgccacg atgtttggca acgcaggtgt gtttgcctgg ttcagctacg taaagccata
    40441 catgatgttt atttccggtt tttcggaaac ggcgatgacc tttattatga tgttagttgg
    40501 gctagggatg gtgctgggaa atatgctaag tggcaggatt tcaggacgtt attcaccact
    40561 gcgcattgca gcagtgactg actttatcat tgtactggca ctgctgatgc tctttttctg
    40621 cggcggcatg aaaacaacgt cgcttatttt tgcttttatt tgttgcgcgg gattatttgc
    40681 cctttcagca cctctgcaaa tattgttact gcaaaatgcc aaaggcggag agttattagg
    40741 tgccgcaggt gggcaaatag cgtttaacct cggtagcgcc gtcggcgcat attgcggagg
    40801 tatgatgctg acgctggggc tggcatataa ttacgtggcg ctgcctgccg ccctgttttc
    40861 gtttgctgcg atgtcatcgt tgctgctgta tggtcgctat aagcgccagc aagcggcgga
    40921 tagtccggtg ctggcgaaac cactggggta gattactgtc acggtgcgcg ttgcttattg
    40981 ccggatgcgg cgtgaacgcc ttatccgccc tacgcggttc tggcacattt tgtaggcctg
    41041 ataagacgcg gcaagcgtcg catcaggcat gatgcgccaa ttgcctacgt tttttactct
    41101 tgtggccata accacgcagc gccgcgtacg ccgctggaat caccgtgctt cgccttactc
    41161 accggcgttt cacattcgcc gccgaagaca aattgtttaa tcaactgccc aaccgtttga
    41221 tataaacggt ctacattgct catcccgccc cccaggacaa tcacatccgg atcgagaata
    41281 ttcacgacat gtgccagcga ttttgccagc cgcagctcgt agcgacgcaa tgccagttcc
    41341 gctaccggat cgctttcttc aaccaggcgg ataatttcac tgcctttcag cgcatgtccg
    41401 ctcaaacgac gataatccgt cgcgaatccc gtgcccgaaa taaaggtttc aatacaacct
    41461 tgtttaccgc aataacaagg gacttcctcg cgataacgca gttcgtcttc gtccatccac
    41521 ggtagcggat tgtgtcccca ctcacctgcc gtgccattgc cgccgatatg cgtccgccca
    41581 ttgaatgcca cgcccgcgcc gcatcccgtg ccgataatca cggcaaatac cgtctgcgct
    41641 cccgctgctg cgccatctac tgcttctgaa accgccagac agttggcatc atttgccagc
    41701 cgcacttccc gctgcaacct cgcgcttaag tctttatcga atggctgacc gttgagccag
    41761 gttgaattgg cattcttcac cacaccggtg tagggcgaaa tcgaacctgg aatgcccata
    41821 cctaccgttc cgcgctgccc tgtcgcctgc tccgccatat caaccaacgt ggcgatcgtt
    41881 tcaatagtct gccggtaatc atcacgcggc gtgggcagac gatggcggta caactgctcc
    41941 cctgcatcgc ccagtgcaat cacttcagtt ttggtgccgc ctaaatcgat acctatacgc
    42001 actgtactct ccttattttt ttcaatatca atagcgtaga gacggacaac cggattggca
    42061 atgcaaggcc gcctacaatt cgttatcatg cccgctaaat ttaacgacaa ggccgtggaa
    42121 attatcatgc tgtggttcaa aaatttaatg gtttaccgtc ttagccgcga gatttcgctg
    42181 cgtgcagaag agatggaaaa acagctagcc tcgatggcat ttaccccatg cggcagccag
    42241 gacatggcga agatgggctg ggttcctccg atgggatcgc acagcgatgc gttaacgcac
    42301 gttgccaatg gtcaaattgt tatctgcgcg cgcaaagaag aaaaaatcct cccgtctccg
    42361 gtgattaaac aggcgctgga agcgaaaatc gccaaactgg aagcggaaca ggcgcgtaag
    42421 ctgaagaaaa ccgaaaaaga ttcgctgaaa gacgaagtgc tgcactctct gctgccgcgt
    42481 gctttcagcc gttttagcca gacaatgatg tggctcgaca cggttaacgg tttgattatg
    42541 gtggactgcg ccagtgccaa aaaagcggaa gatacgctgg cattactgcg taaaagcctg
    42601 gggtcgttac cggttgtacc attgagcatg gaaaacccga ttgaactgac gctgaccgaa
    42661 tgggttcgct ccggtagtgc ggcacagggc ttccagctgc ttgatgaagc cgagctgaaa
    42721 tcgttgctgg aagatggcgg cgtgatccgc gcgaagaaac aagatctgac cagcgaagag
    42781 atcaccaatc acattgaagc cggaaaagtg gtgactaaac tggcgctcga ctggcagcag
    42841 cgcattcagt ttgtgatgtg cgacgatggt tcgctcaagc gtcttaagtt ctgcgacgag
    42901 ctgcacgatc aaaacgaaga tatcgaccgt gaagatttcg cccagcgttt tgatgccgat
    42961 ttcatcctga tgactggtga actggcagcg ttaattcaaa acctgattga aggattaggt
    43021 ggcgaagcac aacgttaatt gctgattttc ctttaatgcc ggatgcgacg cctgccgcgt
    43081 cttatccggc gtacgaagcc acaccaggca tataattatt cgctacggcg agcaataatt
    43141 tttagcgcag caatattatg cgttttacgc cgtaactgat ctaatcgcct catccctatt
    43201 cgcctgccag cagcacgcag aatattcact aacccccatt tatcgctggt aaattgtttt
    43261 gttaacagct ccgcttttgg ggtatcaata tattcacgca ataaatcaga aattgaacaa
    43321 atatccgtca ggtaaggata ccagcaagtt tcaccgtgaa aattacgcac tccagcgtta
    43381 cctgcaataa agtctttcgg gtaatcccag atcacgtttt tatccaacgt gatccaataa
    43441 cgcggtaaat cggtacttcc accattttgg ctgcgcatgg gataacgggt acagtgaatt
    43501 tgcaggttga tcgttggcgt cagtaagtca tagatctccc gctgtaactt gctccatgga
    43561 cgttgtgtca ttgtttttcc tcaagccggt gacgcgcttc actgcgaatt ttccgcgttg
    43621 ccccggtttt cactaaatca cgtaactgcg aaagatcggg ttgttcgaca aataagagta
    43681 atgatggatc ctcttttaac cacgccgttt tcacatcggc agccagctgt ggattattaa
    43741 tagccagcga ccgatcctga ggctcagtca atgtcgttaa caacgacgcc ggggtatgtg
    43801 cgttgcgtaa tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacgccacc
    43861 attcatcatc tgatttatct tttatcgtag ccagttccgc aaaaaatacc tgga
//
LOCUS       DADWTH010000025        43461 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.025, whole genome shotgun sequence.
ACCESSION   DADWTH010000025 DADWTH010000000
VERSION     DADWTH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.025"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..413
                     /locus_tag="JNY02_001422"
     CDS             <1..413
                     /locus_tag="JNY02_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBB9433021.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="JNY02_001423"
                     /pseudo
     CDS             438..3037
                     /locus_tag="JNY02_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="JNY02_001424"
     CDS             3028..4098
                     /locus_tag="JNY02_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9433022.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4110..4652
                     /locus_tag="JNY02_001425"
     CDS             4110..4652
                     /locus_tag="JNY02_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9433023.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4660..5175
                     /locus_tag="JNY02_001426"
     CDS             4660..5175
                     /locus_tag="JNY02_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBB9433024.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="JNY02_001427"
     CDS             5141..5878
                     /locus_tag="JNY02_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBB9433025.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="JNY02_001428"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="JNY02_001428"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBB9433026.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="JNY02_001429"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="JNY02_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBB9433027.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNY02_001430"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNY02_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBB9433028.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNY02_001431"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNY02_001431"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBB9433029.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPSATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="JNY02_001432"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="JNY02_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="JNY02_001433"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="JNY02_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBB9433030.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQLLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="JNY02_001434"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="JNY02_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBB9433031.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="JNY02_001435"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="JNY02_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBB9433032.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="JNY02_001436"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="JNY02_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBB9433033.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNY02_001437"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNY02_001437"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBB9433034.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="JNY02_001438"
     CDS             complement(17760..19520)
                     /locus_tag="JNY02_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBB9433035.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="JNY02_001439"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="JNY02_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBB9433036.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNY02_001440"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNY02_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBB9433037.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNY02_001441"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNY02_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBB9433038.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="JNY02_001442"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="JNY02_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBB9433039.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNY02_001443"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNY02_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBB9433040.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="JNY02_001444"
     CDS             complement(25127..25573)
                     /locus_tag="JNY02_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBB9433041.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="JNY02_001445"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="JNY02_001445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBB9433042.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNY02_001446"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNY02_001446"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBB9433043.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="JNY02_001447"
     CDS             complement(28336..28998)
                     /locus_tag="JNY02_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBB9433044.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="JNY02_001448"
     CDS             29171..29584
                     /locus_tag="JNY02_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBB9433045.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNY02_001449"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNY02_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBB9433046.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNY02_001450"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNY02_001450"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBB9433047.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="JNY02_001451"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="JNY02_001451"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBB9433048.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNY02_001452"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNY02_001452"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBB9433049.1"
                     /translation="MLIFEGKEIEMDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNY02_001453"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNY02_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBB9433050.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="JNY02_001454"
     tRNA            complement(32850..32937)
                     /locus_tag="JNY02_001454"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="JNY02_001455"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="JNY02_001455"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBB9433051.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36253
                     /gene="hyaB"
                     /locus_tag="JNY02_001456"
                     /pseudo
     CDS             34478..36253
                     /gene="hyaB"
                     /locus_tag="JNY02_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36272..36979
                     /gene="hyaC"
                     /locus_tag="JNY02_001457"
     CDS             36272..36979
                     /gene="hyaC"
                     /locus_tag="JNY02_001457"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBB9433052.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36976..37563
                     /locus_tag="JNY02_001458"
     CDS             36976..37563
                     /locus_tag="JNY02_001458"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBB9433053.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37560..37958
                     /gene="hyaE"
                     /locus_tag="JNY02_001459"
     CDS             37560..37958
                     /gene="hyaE"
                     /locus_tag="JNY02_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBB9433054.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37955..38812
                     /locus_tag="JNY02_001460"
     CDS             37955..38812
                     /locus_tag="JNY02_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBB9433055.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38946..40490
                     /gene="appC"
                     /locus_tag="JNY02_001461"
     CDS             38946..40490
                     /gene="appC"
                     /locus_tag="JNY02_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBB9433056.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40502..41638
                     /gene="appB"
                     /locus_tag="JNY02_001462"
     CDS             40502..41638
                     /gene="appB"
                     /locus_tag="JNY02_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBB9433057.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41650..41742
                     /gene="cbdX"
                     /locus_tag="JNY02_001463"
     CDS             41650..41742
                     /gene="cbdX"
                     /locus_tag="JNY02_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBB9433058.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41822..43120
                     /gene="appA"
                     /locus_tag="JNY02_001464"
     CDS             41822..43120
                     /gene="appA"
                     /locus_tag="JNY02_001464"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBB9433059.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43235..>43461)
                     /locus_tag="JNY02_001465"
     CDS             complement(43235..>43461)
                     /locus_tag="JNY02_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HBB9433060.1"
                     /translation="AVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIK
                     GAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10809 a        10588 c        11566 g        10498 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttgctg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacga ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggcgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcggacg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccggact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctagcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagctgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt gaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccaccga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcaggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggatgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagta gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 catttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgcaaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacatat
    34981 ttcttcgacg tacaaaatcg cctgaagaaa ttcgttgaag gcgggcagtt ggggatcttc
    35041 cgcaatggct actgggggca cccgcagtac aaattgccgc cagaagccaa cctgatgggc
    35101 tttgcccact atctcgaagc tctcgatttc caacgtgaaa ttgtcaaaat tcacgcggtc
    35161 tttggcggta aaaacccgca tccaaactgg attgtcggcg ggatgccttg cgccattaac
    35221 attgacgaaa gcggcgcggt cggggcggtc aatatggaac gcctgaacct ggtgcagtcg
    35281 attatcaccc gcacggcaga cttcattaac aacgtgatga tccccgacgc cttagccatc
    35341 ggtcagttca acaaaccgtg gagcgaaatc ggcacgggtc tttctgataa atgtgtcctc
    35401 agctacggcg cattcccgga tattgccaac gactttggtg agaaaagtct gttgatgcct
    35461 ggcggcgcgg tgattaacgg cgacttcaac aatgtgctgc cagtggattt ggttgatccg
    35521 cagcaggtgc aggagtttgt cgatcacgcc tggtatcgtt atcccaacga tcaggtcggg
    35581 cgtcatccat tcgatggcat taccgacccg tggtacaacc ccggcgatgt caaaggcagc
    35641 gataccaaca ttcagcagct gaatgaacag gaacgctact cgtggatcaa agcgccacgc
    35701 tggcgcggta acgcgatgga agtggggccg ctggcacgca cgttaatcgc ttatcacaaa
    35761 ggcgatgctg cgaccgttga gtcggtcgat cgcatgatgt cggcgttgaa cctgccgctt
    35821 tccggtatcc agtcaacgtt gggccgcagg taagttgcag tatttcttcg acaagctgat
    35881 gaccaacctg aaaaatggca atctcgccac tgcttccact gaaaaatggg aacctgcaac
    35941 ctggccgaca gagtgccgtg gcgtcggctt taccgaagcg ccgcgcggag cgttaggcca
    36001 ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg
    36061 gaacgccagc ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa
    36121 caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga
    36181 tccgtgcctc gcctgttcaa ctcatgtgct gggcgacgac ggtagcgagc tgatctccgt
    36241 gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag
    36301 ccactatgtc tttgaagcac cagttcgtat ctggcactgg ctgacggtgt tatgcatggc
    36361 ggtgttgatg gtcaccggtt actttatcgg caagccgcta ccttccgtca gtggcgaggc
    36421 gacgtatctg ttctatatgg gttacatcag gttaattcac ttcagcgccg ggatgatttt
    36481 taccgtggtc ttgctgatgc ggatctactg ggcttttgtt ggcaatcgtt actcccgcga
    36541 gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg
    36601 ctggtatctg tttctggcaa aacgtccgag tgctgatata ggccataacc ccatcgccca
    36661 ggcggcgatg ttcggctatt tcctgatgtc ggtctttatg atcatcaccg gttttgcgct
    36721 gtacagcgaa cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt
    36781 ctactggacg ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct
    36841 gattggggcg tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga
    36901 cgacacggtg atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag
    36961 taacaaggag cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg
    37021 tgggccgatg aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg
    37081 cccgagtatg tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc
    37141 gaaagcgcca gccatctgtt gattctcgat gccattgact acgggctgga acctggaagg
    37201 ctgcgaacct atgccggaga acgcattccg gcctatctca gcgcgaagaa aatgagcctg
    37261 catcagaaca gtttctccga agtgctggcg ctggcagata tccgcggaca tctgccagca
    37321 catattgccc tcgtcgggct gcaacccgcg atgctcgacg actacggcgg cagcctgagc
    37381 gaactggcac gggagcaact gcccgctgcg gaacaatcgg cgctggcgca gttggctgcg
    37441 tggggaagtg tgccgcaacc ggctaatgaa tcgcgttgtc tcaattatga ctgtctgtcg
    37501 atggaaaatt acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga
    37561 tgagcaacga cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc
    37621 cggtcagtga atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat
    37681 taagcagtga cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat
    37741 tactgcacga gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg
    37801 aagccatcgg cgatcgcttt ggcgcctttc gctttcctgc cactttagtg tttaccggcg
    37861 gaaactatcg cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc
    37921 gcgggcttgt cgaaccgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct
    37981 ggggccagga acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg
    38041 tcaggtgaat gatgaaccga gtatggcggc cttggagcaa tgtgctcaca gcccgcaggt
    38101 gattgcgctg ttaaacgagt tacaacatca actaagcgaa cgccagccgc cgttgggcga
    38161 ggtgctggca gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct
    38221 tctcggggaa ggggaagtgt cagtgcgcat tcagcaggct gacgacagta aaagtgaaat
    38281 acaggaggcg atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct
    38341 ggaggacaaa ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa
    38401 tctcttgccg acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc
    38461 gttggcgcat gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa
    38521 tctgacgcaa ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc
    38581 gggaaatatt cagattcgta ccattggcta tggcgagagc tatatcaact ccacggggtt
    38641 acgccatgtc tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta
    38701 tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc
    38761 gcagcggctt agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg
    38821 taagtatcgc tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt
    38881 tcaggataaa gagggagatc taacattatc gggttatttt tctctcttcg cctacaggag
    38941 tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc
    39001 actttttatt tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca
    39061 tttacgtggt caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct
    39121 tcggtatcaa ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta
    39181 ctaactggtc attctattcc aactatgtgg gcgatatttt tggcgcacca ctggcgatgg
    39241 aagcattaat ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc
    39301 aacggctgaa taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc
    39361 tctctgcatt gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt
    39421 ttgatatcga caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg
    39481 tcagccaggt gaaatttgtg cacaccgtca tggcgggcta cgtgaccggg gccatgttta
    39541 ttatggcgat cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgct
    39601 cgtttgccat cggttccgtc ttcggtactc tggcgattat cggtaccctg caactcggag
    39661 acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg
    39721 agtggcaaac ggaacctgct cctgcaccgt tccatgtggt tgcctggccg gaacaggatc
    39781 aagagcgtaa cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact
    39841 cattagataa acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc
    39901 aacgcggacg tatggcctgg ctgttaatgc aggaaatatc tcaaggcaat cgtgagccgc
    39961 atgtgttgca ggcattccgg gagctggaag gcgaccttgg ttacggcatg ttgctctccc
    40021 gctatgcgcc ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg
    40081 cgatacctca ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc
    40141 tgctgctact ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc
    40201 atcgctgggt gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag
    40261 ccgggtggtt tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga
    40321 catactccgc gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg
    40381 taggtcttta caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc
    40441 tggggccgag cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat
    40501 catgtttgat tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt
    40561 ggtctttatg atctccgacg gatttgacat ggggatcggc tgtttgctgc cgctggtggc
    40621 gcgtaatgat gatgaacgcc ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa
    40681 tcaggtctgg ttgatcctcg caggtggggc attatttgcc gcctggccca gagtgtatgc
    40741 agcggcgttt tccggctttt atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg
    40801 tccgctggcc tttgattatc gcggaaaaat tgccgatgca cgctggcgta aaatgtggga
    40861 tgccggtctg gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa
    40921 cttgttgctc ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atctcggcag
    40981 cttctggcaa ctgctgacgc cattcccttt attgtgtggt ttgctcagcc ttgggatggt
    41041 gattttgcaa ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc
    41101 acagctggcg actaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcaggtta
    41161 ctggctgtgg gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc
    41221 caatccgtta atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga
    41281 atcgcccgtt ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat
    41341 ggcgatttat cgtggtcgcc cgggttgggg atttttgatg gcatcattga tgcaatttgg
    41401 cgtgattttc acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gtgtgagtcc
    41461 aatctccagc ctgacgttgt gggacagcac ttccagtcag ctgacgctga gcattatgtt
    41521 ggtaatcgtg ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa
    41581 aatgtgggga cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg
    41641 agcaggaaca tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc
    41701 acccttgtgt tggtatggct ggacctgcgt ctgaaaagtt aacgaacgta ggcctgatgc
    41761 ggcgcattag catcgcatca ggcaatcaat aatgttagat atgaaaagcg gaaacatatc
    41821 gatgaaagcg atcttaatcc catttttatc tcttctgatt ccgttaaccc cgcaatctgc
    41881 attcgctcag agtgagccgg agctgaagct ggaaagtatg gtgattgtca gtcgtcatgg
    41941 tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc
    42001 aacttggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctatct
    42061 cggacattac caacgccagc gtctggtagc cgacggattg ctggcgaaaa agggctgccc
    42121 gcagtctgct caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga
    42181 agccttcgcc gccgggctgg cacctgattg tgcaataacc gtacataccc aggcagatac
    42241 gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataacgc
    42301 gaacgtgact gacgcgatcc tttgcagggc aggagggtca attgctgact ttaccgggca
    42361 tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat ttcccgcaat caaacttgtg
    42421 ccttaaccgt gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact
    42481 caaggtgagc gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac
    42541 ggagatattt ctcctgcaac aggcacaagg aatgccggag ccggggtggg gaaggatcac
    42601 cgattcacac cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttactaca
    42661 acgcacgccg gaggttgccc gcagtcgcgc caccccgtta ttggatttga tcatggcagc
    42721 gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct
    42781 gtttattgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg
    42841 gacgcttcca ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg
    42901 gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca
    42961 gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac
    43021 cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca
    43081 aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca
    43141 gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa
    43201 aaggcgttca cgccgcatcc ggtcactttc agttttactc tttctcggag taactataac
    43261 cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga
    43321 ttgcgccttt aatattaacg ccagcctgtt ccagacgctg cattgacaag ctcacctctt
    43381 tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccgacagag cgtcccacga
    43441 ccgcagcatc gctcaccgcc a
//
LOCUS       DADWTH010000026        40384 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.026, whole genome shotgun sequence.
ACCESSION   DADWTH010000026 DADWTH010000000
VERSION     DADWTH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.026"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..789)
                     /locus_tag="JNY02_001466"
     CDS             complement(<1..789)
                     /locus_tag="JNY02_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBB9433061.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1036..1662
                     /gene="gstB"
                     /locus_tag="JNY02_001467"
                     /pseudo
     CDS             1036..1662
                     /gene="gstB"
                     /locus_tag="JNY02_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1659..2773)
                     /gene="yliI"
                     /locus_tag="JNY02_001468"
                     /pseudo
     CDS             complement(1659..2773)
                     /gene="yliI"
                     /locus_tag="JNY02_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNY02_001469"
     CDS             complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNY02_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBB9433062.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3480..4805
                     /gene="rimO"
                     /locus_tag="JNY02_001470"
     CDS             3480..4805
                     /gene="rimO"
                     /locus_tag="JNY02_001470"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBB9433063.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4852..6180)
                     /locus_tag="JNY02_001471"
     CDS             complement(4852..6180)
                     /locus_tag="JNY02_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBB9433064.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVESINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6188..8536)
                     /locus_tag="JNY02_001472"
     CDS             complement(6188..8536)
                     /locus_tag="JNY02_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBB9433065.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFCVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8714..9625)
                     /gene="gsiD"
                     /locus_tag="JNY02_001473"
     CDS             complement(8714..9625)
                     /gene="gsiD"
                     /locus_tag="JNY02_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBB9433066.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9628..10548)
                     /gene="gsiC"
                     /locus_tag="JNY02_001474"
     CDS             complement(9628..10548)
                     /gene="gsiC"
                     /locus_tag="JNY02_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBB9433067.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10566..12104)
                     /gene="gsiB"
                     /locus_tag="JNY02_001475"
     CDS             complement(10566..12104)
                     /gene="gsiB"
                     /locus_tag="JNY02_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBB9433068.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVIPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(12124..13995)
                     /gene="gsiA"
                     /locus_tag="JNY02_001476"
     CDS             complement(12124..13995)
                     /gene="gsiA"
                     /locus_tag="JNY02_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBB9433069.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13982..14947)
                     /gene="iaaA"
                     /locus_tag="JNY02_001477"
     CDS             complement(13982..14947)
                     /gene="iaaA"
                     /locus_tag="JNY02_001477"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBB9433070.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMESVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15151..16386
                     /gene="moeA"
                     /locus_tag="JNY02_001478"
     CDS             15151..16386
                     /gene="moeA"
                     /locus_tag="JNY02_001478"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBB9433071.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16386..17135
                     /gene="moeB"
                     /locus_tag="JNY02_001479"
     CDS             16386..17135
                     /gene="moeB"
                     /locus_tag="JNY02_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBB9433072.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17314..17976)
                     /gene="fsa"
                     /locus_tag="JNY02_001480"
     CDS             complement(17314..17976)
                     /gene="fsa"
                     /locus_tag="JNY02_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBB9433073.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            18107..19006
                     /locus_tag="JNY02_001481"
     CDS             18107..19006
                     /locus_tag="JNY02_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBB9433074.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19012..21444
                     /locus_tag="JNY02_001482"
     CDS             19012..21444
                     /locus_tag="JNY02_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBB9433075.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21590..22405
                     /gene="ybiV"
                     /locus_tag="JNY02_001483"
     CDS             21590..22405
                     /gene="ybiV"
                     /locus_tag="JNY02_001483"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBB9433076.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22557..23822
                     /locus_tag="JNY02_001484"
     CDS             22557..23822
                     /locus_tag="JNY02_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBB9433077.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24063..25655)
                     /locus_tag="JNY02_001485"
     CDS             complement(24063..25655)
                     /locus_tag="JNY02_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBB9433078.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25874..26794
                     /gene="ldtB"
                     /locus_tag="JNY02_001486"
     CDS             25874..26794
                     /gene="ldtB"
                     /locus_tag="JNY02_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBB9433079.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26852..27970)
                     /locus_tag="JNY02_001487"
     CDS             complement(26852..27970)
                     /locus_tag="JNY02_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBB9433080.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFQTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27967..28434)
                     /gene="mntR"
                     /locus_tag="JNY02_001488"
     CDS             complement(27967..28434)
                     /gene="mntR"
                     /locus_tag="JNY02_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBB9433081.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28620..28748
                     /gene="mntS"
                     /locus_tag="JNY02_001489"
     CDS             28620..28748
                     /gene="mntS"
                     /locus_tag="JNY02_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBB9433082.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29020..30603
                     /gene="opgE"
                     /locus_tag="JNY02_001490"
     CDS             29020..30603
                     /gene="opgE"
                     /locus_tag="JNY02_001490"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBB9433083.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYKTFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30651..31166)
                     /gene="ompX"
                     /locus_tag="JNY02_001491"
     CDS             complement(30651..31166)
                     /gene="ompX"
                     /locus_tag="JNY02_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBB9433084.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGLNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31219..31284)
                     /locus_tag="JNY02_001492"
     CDS             complement(31219..31284)
                     /locus_tag="JNY02_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433085.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31519..32406
                     /gene="rhtA"
                     /locus_tag="JNY02_001493"
     CDS             31519..32406
                     /gene="rhtA"
                     /locus_tag="JNY02_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBB9433086.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32705..33208
                     /gene="dps"
                     /locus_tag="JNY02_001494"
                     /gene_synonym="pexB"
     CDS             32705..33208
                     /gene="dps"
                     /locus_tag="JNY02_001494"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBB9433087.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33612..34358
                     /gene="glnH"
                     /locus_tag="JNY02_001495"
     CDS             33612..34358
                     /gene="glnH"
                     /locus_tag="JNY02_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBB9433088.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            34497..35156
                     /gene="glnP"
                     /locus_tag="JNY02_001496"
     CDS             34497..35156
                     /gene="glnP"
                     /locus_tag="JNY02_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBB9433089.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35153..35875
                     /gene="glnQ"
                     /locus_tag="JNY02_001497"
     CDS             35153..35875
                     /gene="glnQ"
                     /locus_tag="JNY02_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBB9433090.1"
                     /translation="MIEFKNVSKHFGSTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36136..38360
                     /gene="ybiO"
                     /locus_tag="JNY02_001498"
                     /pseudo
     CDS             36136..38360
                     /gene="ybiO"
                     /locus_tag="JNY02_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38357..39283)
                     /gene="rlmF"
                     /locus_tag="JNY02_001499"
     CDS             complement(38357..39283)
                     /gene="rlmF"
                     /locus_tag="JNY02_001499"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBB9433091.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39559..39819
                     /locus_tag="JNY02_001500"
     CDS             39559..39819
                     /locus_tag="JNY02_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBB9433092.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            40084..>40384
                     /locus_tag="JNY02_001501"
     CDS             40084..>40384
                     /locus_tag="JNY02_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBB9433093.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10048 a        10679 c         9825 g         9832 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ctcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
      841 taagttcggt gattaataac gcacacacaa acttaaagaa accgtaagtt ggtaaagttt
      901 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
      961 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagtctgtg gctgcctgtc
     1021 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaactaa cgtaaaaaaa
     1081 gtattgctga cgctcgaata actggaacta ccttatgagc aaattctcgc gggccgtgag
     1141 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
     1201 ttacgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
     1261 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg
     1321 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
     1381 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
     1441 gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctct
     1501 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
     1561 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
     1621 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
     1681 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
     1741 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
     1801 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cccaataaat aatttttgct
     1861 gccactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg ggcgaatctt
     1921 tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct tccggtatct
     1981 taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt ttgccttttt
     2041 gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac agtgcattac
     2101 tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag atctcggcgc
     2161 gcgcaccgga ttcctttata aaaggattat catccgggat ttcgccctgg tcggtcagac
     2221 gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga ttgttttcgc
     2281 ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca aaatggttgc
     2341 cggtagacag ttttggcatc tggcggaaga cggtgcggaa gtcggtcact tttgagagat
     2401 catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg ccaacttcgg
     2461 aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc acgtccagca
     2521 ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca gataatcctt
     2581 ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata ccgtgattat
     2641 cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact tcgacattta
     2701 ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca aggaaaaagg
     2761 attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca gtggaggaga
     2821 taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc tcccccttag
     2881 ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca gcgttaagct
     2941 catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc aatcatggtg
     3001 cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat gaagagcact
     3061 ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt
     3121 cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt atccttttgc
     3181 cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag
     3241 atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc cttgttgcaa
     3301 ttaaatagta tggctatttt ttgagcaaaa tgctgtctag gatcaattct tcacgccgaa
     3361 ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat acaaatttta
     3421 tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt ttttaagcta
     3481 tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc
     3541 ttgtcgattc agagcgtatt ctcaccgaac tccgcactga aggttatgac gtggtaccga
     3601 gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac agcgcggtac
     3661 aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg
     3721 gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca
     3781 ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg ccaaaaccga
     3841 aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg ccgcgtcatt
     3901 atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc attattccgt
     3961 ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc
     4021 tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc gcctatggcg
     4081 ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc agcatggtca
     4141 gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac gtttaccctt
     4201 atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg ccgtatctgg
     4261 acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt ccgggttctg
     4321 tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac
     4381 gctcaacctt tattgtcggc ttccctggcg agacggaaga agatttccag atgctactcg
     4441 acttcctgaa agaagcacgt ctggatcgcg ttggctgctt taaatacagc ccggttgaag
     4501 gtgcagacgc caatgccctg cctgaccagg ttccggaaga agtgaaagaa gaacgctgga
     4561 accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag aaagtgggcc
     4621 gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg
     4681 cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac attaagccgg
     4741 gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg
     4801 tttaattaat tcaggccggg caaaatcatt tgtccggcct gcgaataatg attactgcca
     4861 gctaatctgt gccctgccgt ttttcttact cttatacagc cgctcatccg ccagcatttt
     4921 caagcgttcg acggtagtac atttatttga atgcgctaca ccgatactgg tgcttaaatt
     4981 aatgctttcc acctggaaat gaacgatttc ttttagtatc gatagcaact cttccagcgt
     5041 gccgccagta taaatcaccg caaattcgtc acctccgaag cggtagactt tacctttatc
     5101 taccgggatc tgtgatttaa ggattttcac cactttcatc aacacttcat cgcccagcaa
     5161 atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg tcaaacagac acaaataacc
     5221 gcctttttcc ggcagcgctt ttaaatcttc atcaaaggat tttctgccca ttgctttggt
     5281 caggtcgtcc aggaacgccg agttatacat actgtagagt tttttgacat cggcagccga
     5341 aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta ttaatgatgg cttctttctc
     5401 cacaatacca cgattaatca ctctgcgtac cagatagagg caactggcac ccgatatcac
     5461 tgccaccaat gcggcaaaga aaataatcaa tgtacaaacc cgcaaataaa catctttttt
     5521 atacgcaaat acgtacaatc cggtcggcaa ataggcgaag tgctccatcg cttccgaaac
     5581 ggtgaagatg gcgtttttag cattatattc atcaccggtc accggtttac cgttcttaaa
     5641 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
     5701 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
     5761 gaaggtttta ccatcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
     5821 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
     5881 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
     5941 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
     6001 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
     6061 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
     6121 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
     6181 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
     6241 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
     6301 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
     6361 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
     6421 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
     6481 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
     6541 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
     6601 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
     6661 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgttga ctaaaatgtc
     6721 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
     6781 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
     6841 ggcacaacgc ctccgcgctc agaatggcgc gggtgttaat atcaacgatc ggttgataga
     6901 cgaggaatag ttcgccaatt tccattgcat ggcgaatatt acgcgcaatc cagatattct
     6961 cttctatttc gttgagtttc gcgtcatcac agaaaataaa ctcggagaac atatggtcac
     7021 ggatactgga aatcgccagt ttcagttttt ctgcgtaact ccaggcgttt tccccctgca
     7081 aaggttcaca aatggcagac ttatggatgc gcagattgcg gtagccaaag ctgctgtcaa
     7141 aatcgtttac catcgctatt ttacgggtaa atgtctgtac attttcccga cacagaagta
     7201 aatagagatc ggcattgaat gtcacgatat cgcagcattg cgcatatttc tcggcaattc
     7261 gctgacacaa tttcctgacg attttattgc gttcatcaac acaaaacacc gcttccacgg
     7321 cttccagacc acacagtttg atgatcccca cagccagata ctgattattg ttggactcaa
     7381 taatcgccat atttttaata aaaccgcttt cctgatcgta agagatcagt gagagcaatt
     7441 tatttgaccg ttcaatttcg gcatctttca tttccgcgat cgaatttttg atgctgataa
     7501 tctctttcgc atcgccttcg cgaattttca gagcctcgat ctgatccgtt gagtcgggca
     7561 aaaacgccag cgtattaacc agctcggtca tttcagaaaa ttgccgacgg aacacacgag
     7621 tggtcatgaa atataccacc agcaacacga taataatgaa cgcaaacggt agataaaaaa
     7681 gtttatctga tgcgttcaat aaattaccgc caggataata aaaatagatt tcgaacaatg
     7741 cattatttgg cactgctgat gtctttgcaa tatactccgt atcgacagaa tcaataatat
     7801 tataattacc ttctctatta taaataatca gtttttcacg agcgagttga ttcttggcaa
     7861 tgatctcatg accttgtttc aaaaatagaa cttcatcatt agcgctgatt tgatcaagat
     7921 actcgttcag ataccgccag tttaaatcga catgaatttt atctaccgct aacaaatcgt
     7981 gatctttgcc gttgatacgt agcgtaaaag agatacattt tttacccgga cgatcaaagc
     8041 aattgtatgc cggattccag ctgttgttag agtcatctga agccaccgac cacggtcgtt
     8101 tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc ggtccattta
     8161 cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag agataatatt
     8221 tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc attttcagaa
     8281 tcacggcgcg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc tttaatgtca
     8341 gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata agttcaaagc
     8401 ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatc atgaaaaaaa
     8461 gaccaataaa tgacagtgct gccaataaaa ataaaattat cagccttatc tttatctttt
     8521 cgtataaact cagcatgtta atacatttaa caaaataact atctgaacgc tgtgccgctg
     8581 caaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca aaatcagcag
     8641 aaagttacaa cacttgctta gggcaataat ttcctgtgag gtgattaccc tttcaagcaa
     8701 tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca
     8761 acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg
     8821 gcgcgataac catatccgct cgtgcctcat tgagcattgc tccccactct ggtgtcggcg
     8881 gctgcgcacc taggccgaga aatgacaggc tggcggcaga gataatcgag gtgccaatgc
     8941 gcatggtgaa aaacaccacg atagaagaga cggtcccagg caggatatga cgcaataaaa
     9001 tggtcatatc gctggcacca atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca
     9061 ccagcgtgtt gccgcgcacc aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga
     9121 taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac
     9181 ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag ccgatcccac cagccttcat
     9241 aatatccagc caacaagccc agcaacgtcc ctatcgccac accgataaac acggcaaaca
     9301 cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca
     9361 gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt attcagattg tcataatcaa
     9421 aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc acaatcaata
     9481 aaataacgaa taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt
     9541 catgccacgg tgtacgtacc tggtcaggtt tgaccagtgg catggcgttt aacaccgcct
     9601 gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt taatggcggc
     9661 gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa gcagaatttc
     9721 cgcctgaatc accgggtaat cgcgcatttc tacagagtca acgagtaagc gtccaagtcc
     9781 gggccagttg aaaactttct ccaccacgat agaacctccg agcaaaaagc caaactgtaa
     9841 gcccatcatg gtcactaccg ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca
     9901 ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta aaacgtcgac
     9961 aaatgacgca cgggtaaagc gcgccatcac ggcggccact gccgcgccga gcgtcaggga
    10021 gggtaaaatg tagtgctgcc aactgtctgc tcccacagta ggcagccagc ccagttcaac
    10081 ggagaatacc tgaattaaaa acatccccag cgcaaatgcc ggaaacgaga tccccgacac
    10141 cgcaatggtc atactcaatc gatccggcca acgattacgc cagacggcgg cgataattcc
    10201 cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc acagcgttgg
    10261 cataaagcgg ctggcaatct catcggcaac cggacgacgc gacaccatcg acaggccaaa
    10321 atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg gctgatccaa
    10381 ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgggcc cggcaatcaa
    10441 tcgcgccgga tcgccgggca gcatgtggac aaataaaaac accagcaccg agacgataaa
    10501 cagcgtcgga atcaacccca gtaatcgttt gataacgtaa ttaagcattc cactccctgc
    10561 gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgc tgtctggcat gatccaaaaa
    10621 ccggtcaggt ttttactgtg tgccgacacc agtttttcta ccaccagcgg gatccacggc
    10681 gattcttgcc agatgatatc ctgcgccgcc ttatataagc gggtcttttc cgccggatca
    10741 ttcgttttca gtgcctgagc caggaagtca tccacctgtt tattgctgta aaacgcggta
    10801 ttaaacagcg tcggtggcca gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct
    10861 tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc tttttgccct
    10921 ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cggtcacctg ggctttaatc
    10981 ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta
    11041 tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt cagtaattcg
    11101 cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc gatacttggc
    11161 ggtatcacac cagtagctgg cgttgcatag cccgcaaagg caactttcac cagcgccgga
    11221 cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc
    11281 acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc gatatttttg
    11341 tttttctcca gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg
    11401 gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc
    11461 agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc
    11521 tgattccagg tatccagttc atacggtccg gttcccaccg gatgaaaacc aatctccttg
    11581 ccatattttt ccagcgctgt cggtgaaatc atcgcggtcg ccggatgggc aagaatatta
    11641 ataaacgctg agaacggctg tttgagggta atctttaccg ttgtcggatc gatcgcctcc
    11701 gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg atcgctggcc
    11761 cggtccagat tcgcttttac cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt
    11821 ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg aaacggtata actctccgcc
    11881 agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta aaacgatttc
    11941 gctacggcct gagataacgt gtcattcgcg tcatacggat cgagcgtggt gaaattcgat
    12001 cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa cgctgtcgca
    12061 atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt ctccgcctga
    12121 atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat gccccggccc
    12181 gacgcattgc aacgagacgg ctgccacctc ttcgccacgc agatgaatat tgctgggaag
    12241 atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc
    12301 cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc accgtgaacc
    12361 aatttcaaca atttgcccga gatacatcac cgccacacga tgactaatcc gctctaccac
    12421 agccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga gatcgagcaa
    12481 caagttgata atctgcccgc gaatagaaac atccagcgcc gaaacggctt cgtcggcaat
    12541 gatcactttt ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc
    12601 ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac aggcccacgc gctccagcaa
    12661 ccacgcaacg cgtgcagccg cgtctttacc tggcaataaa ccgtgtacac gcagcggttc
    12721 gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat
    12781 aaactgaata tcccggcgca atgcctgaag tttgccaggt gacaaggtat cgattcgctg
    12841 accgttaaag ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt
    12901 agtggattta ccgctgccag actcgcccac cagcgatagc gtttcgccag gccagagatc
    12961 aaaactgact ttctcaacgg catgcacttc ccgcgttacg cgattcaaca aaccgctgcg
    13021 caaagggaaa cgggtgacaa gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt
    13081 tttctgctcg atggggggtt cctgtttcgc tggatgttca agcgatatca atgggaaacg
    13141 tcggggataa tctaaccctt tcatcgcacc aagttgcgga acagcagcta acagcgcacg
    13201 ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc
    13261 gccctgatac atcaccagta cccgatcggc aatctctgcc accacgccca tatcgtgagt
    13321 gataaagata acgcccatcg acatctcttt ttgcaatact ttgattaatt gcaggatctg
    13381 cgcctgaata gtgacatcca gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg
    13441 gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga gttgatgcga
    13501 ataacgtgaa agaatggttt gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc
    13561 ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga cgaattgatt cggcaatctg
    13621 ttcacccaca gtaaatactg ggttcagcga tgtcatcggc tcctgaaata tcatcgccat
    13681 atccgcaccg cgcacatggc gcatttgtgc agcgctctgc tcgctaagtt caatcacatc
    13741 acgactgcgc cgccgcaaca gcattttatc gcactgtact aaaccgcccg cctgttccaa
    13801 cagacgcatc aatgccagcg cagtcactga cttaccggag ccggattcgc ctacaattgc
    13861 cagcgtctca ccgcgttgca gactaaaaga gagattgcgg accgcagcta ttttctgctg
    13921 gtcctgcata aaggcaatat taagattttc aaccgccagc acattaccgg catcaagttc
    13981 atcactgtgt ggcaacggtg tccccttttt cacggtagat accggtggtt ggcgtatcgc
    14041 ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg acattccctt
    14101 catggtcgat agcgattaag ccaccgctac cgccaagcgc agggagtttt tccattacta
    14161 cccgctcgca ggcttccgcg agacttaatc cgccgtaatc cattaacgcg gcgatgtcat
    14221 atgccgccag cgcgcggatg aagacttcgc ctgtgccggt acaagaaacc gccacactgg
    14281 cgttattggc gtagcatccg gcacccacta agggactatc gccaactcgt ccgggtaatt
    14341 tattggtcat tccgcctgtg gacgtggctg ccgccagatt gccgtctaaa tccaacgcca
    14401 cggcccccac ggtgcccatt ttctgttttt catccagtgg cgcaccgcta tggtcgagga
    14461 ctgttgcccc ttcctcgcgc gctgccagta gttgttcata acgcaaaggc gtggagaaaa
    14521 tctccggtga gacgctctcc atgccatgag caaacgcaaa attttctgcc ccttcgccaa
    14581 tcatcatcac atgcgggctt tgctccatca ccagccgggc ggcaagaacc ggattacgca
    14641 gatgactaac gcccgccacc gcaccggctt tcagggtgtt accatccatc acacaggcgt
    14701 ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta aacagtggac
    14761 actcttccag cagacgcacc gcttccgtca ccacatccag cgcactttcg ccggcttcca
    14821 gcattttctg cccggtttca acaatggcag acaacgcctc gatgtagcgt aattcctgtt
    14881 gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc atgaattgca atgactgctt
    14941 tgcccatagt attcgtccat taagggtggc tcgccacttt tgctataaat atcagaattt
    15001 ttatgaatta ctcattcaat ttttgagtat agaaagatgg atatggcatg taaaggcagg
    15061 agccaacgct acgcccgagg tggcatacag tctatggcgt ttttctgaca taataggcaa
    15121 attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt gatgtcgctc
    15181 gacaccgcgc ttaatgagat gctttctcgc gttaccccac tgaccaccca ggaaacgctg
    15241 ccactggttc agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc
    15301 ccggggtttg ataactccgc tatggacggc tacgcggtgc gtttagccga tattgcctcc
    15361 gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc agccatacca tggtgaatgg
    15421 cctgcgggta cctgcattcg tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg
    15481 gtggtgatgc aggagcagac tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg
    15541 cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc
    15601 ccggcgggaa ctcgcctgac taccgcagag ctgccagtaa ttgcttcact ggggattgcc
    15661 gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga tgaactccag
    15721 ttgcccggtc agccgctggg cgacggccaa atctacgaca ccaaccgtct cgccgtacac
    15781 ctgatgttag aacagctggg ttgcgaggta atcaacttag gaattatccg cgacgatcca
    15841 catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtagt gatcagttcc
    15901 ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga gctgggggag
    15961 atcgccttct ggaagctggc gattaaacca ggtaaaccgt tcgcattcgg taaactcagc
    16021 aatagctggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac cttctatcaa
    16081 ctggtacagc ctttgctggc aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc
    16141 cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc
    16201 ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg acatcagggt
    16261 tcacatatat ttagctcctt tagtctcggc aactgcttta tcgtgctgga acgcgatcgc
    16321 ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc
    16381 ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa atcattctgc
    16441 gcggttttga ttttgacggt caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc
    16501 tcggtggact cggctgcgca gcttcgcagt atctggcaag cgccggtgtc ggcaacctga
    16561 cgctgctcga cttcgacacg gtttcgctct cgaatctgca acgccagaca ctgcacagtg
    16621 atgccacggt cgggcaaccg aaggtggaat ccgcccgtga cgccctgacg cggatcaacc
    16681 cacatatcgc gattacgcca gtcaatgcac tgctagatga cgcagaactt gcagcattga
    16741 ttgctgaaca cgatctggtg ctcgactgta cggataacgt tgcggtacgt aatcaactga
    16801 acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag
    16861 ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt
    16921 tgtttggcga gaacgcgtta acctgcgtag aagcaggcgt gatggcaccg ttgatcggcg
    16981 taattggttc gttgcaggca atggaagcga tcaaactgct ggcaggttat ggcaaaccgg
    17041 ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa atgaaactga
    17101 tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc ctgataagac
    17161 gcgacaagcg tcgcatcagg catttgctcc agacgccaga tgcggcgtga acgccttatc
    17221 tgccctacgg ttcgggcaca gatttccaag cgcggcaggc atcggtgcac tccgccacgt
    17281 catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc caaacgctcc
    17341 ctgccagtcc tgctcaaact tcgccacagc ggcttccacc gccggatagc taatcatctg
    17401 ttgtgccaca tccagtggca gagtaattga ttcacatccg gccagtaagc agtccagcgc
    17461 ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat gcattttcaa
    17521 taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat caatacgatt
    17581 aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc
    17641 atataccgcg gttcccagcg tcggaatccc ttccgctttt aacatcttaa tcgctgccag
    17701 cccctcggct gtcaccggaa ctttcaccac gatatccgca ataatagaac gcagcttaag
    17761 cgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca gacgcccctg
    17821 accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggttttt tacccgcggc
    17881 gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca gcgccttcac
    17941 cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa tgtacttttt
    18001 gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat caatctaact
    18061 ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga ttttcaatat
    18121 tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc ttaaaggctg
    18181 ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct
    18241 gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg cgccggaagt
    18301 gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc cggagcatct
    18361 gacggcgtta accgactgct gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag
    18421 cgttgaggag atcatggcga ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg
    18481 cggcttaacg ctttcgggtg gtgaaccctt catgcagccg gaaatggcga cggcgctgct
    18541 gcaagcctgc cacgaggcag gcattcatac tgcggtagaa acctgtctac atgtgccgtg
    18601 gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt taaaacacgt
    18661 tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagggtgc tggataacct
    18721 gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga ttcagggctt
    18781 taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg agctgcacgt
    18841 tggcgaaatt cattttctgc cctaccacac gctgggcatc aacaaatatc acttacttaa
    18901 tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gagctgctcg attttgccca
    18961 gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac catgaccaca
    19021 ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt
    19081 gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta tcaacaacat
    19141 ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc
    19201 accatctgga tcaaacacta tgagttgatc attggcaacc aggcaagcga agttcgcgcc
    19261 gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga tgatctggca
    19321 gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg
    19381 tgcccgtggt ggcgcggtca gacagtacag gatcgctgct atggcatgtt taccgatgag
    19441 caaaaaggtc tgctggcgac cggcatcatt aaagcggaag gcaatatgac ctccggcgat
    19501 gcgcacctgg cggtcaattt cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag
    19561 aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa
    19621 cagttcctga aagcgattga tatcgtgctg gtggcagtca gtgaacacat tgaacgtttc
    19681 gctgccctgg cgcgtgcaat ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg
    19741 ctggcgatgg cagaaaactg cgatcttatc gcccaccagc cgccgcagac tttctggcag
    19801 gcgctgcaac tgtgttactt catccagttg attttgcaga ttgaatctaa cggtcactcg
    19861 gtgtcgtttg gtcgtatgga tcagtatctc tacccgtact atcgccgcga cgttgaactg
    19921 aaccagacgc tggatcgcga acacgccatc gaattgctgc atagctgctg gttgaaattg
    19981 ctggaagtga acaaaatccg ctccggctcc cactcaaaag cctctgcggg aagtccgctg
    20041 tatcagaacg tcaccattgg cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg
    20101 aatccactct cttacgcgat cctcgaatcc tgcggtcgcc tacgttccac tcagcctaac
    20161 ctcagcgtgc gttaccatgc aggaatgagt aacgatttcc tcgacgcctg cgtacaggtg
    20221 atccgctgcg gcttcgggat gccggcattc aacaacgacg aaatcgtgat cccggaattt
    20281 attaaactcg gtattgaacc gcaggacgct tatgactacg cagcgattgg ttgtatagaa
    20341 accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat caacttcgcc
    20401 cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg
    20461 ccacaagaaa aagcgttgtc ggcaggtaac ttcaacaact tcgatgaagt gatggccgcg
    20521 tgggatacgc aaatccgtta ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac
    20581 accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga tgactgtatt
    20641 gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc tggcctgcag
    20701 gtcggtattg ccaacctcgg caacagcctg gcggcagtga agaaactggt gtttgaacag
    20761 ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga tggcctgact
    20821 catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa cgacgatgat
    20881 accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact gaaacagtac
    20941 cataacccac gctacggtcg tggtccggtt ggcggcaact attacgcggg tacgtcgtct
    21001 atctccgcta acgtaccgtt tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc
    21061 cacaccccgc tggcagaagg cgctagtccg gcctctggta ctgaccatct cggccctact
    21121 gcggtcattg gctcagtggg taaactgcct acggcagcga ttctcggcgg cgtgttgctc
    21181 aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa actgatgatc
    21241 ctgctgcgta ccttctttga agtgcataaa ggctggcata ttcagtacaa catcgtttcc
    21301 cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt
    21361 gtcgcgggtt attccgcctt cttcaccgcg ctctctccag acgctcagga cgatatcatc
    21421 gcccgtactg aacatatgct gtaatctctt cggcccggct gcggcgtcgc cgggctaaat
    21481 cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg ctctgcgtca
    21541 cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta tgagcgtaaa
    21601 agttatcgtc acagatatgg acggtacttt tcttaacgac gccaaaacgt acaaccaacc
    21661 acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attaagttcg ttgtcgccag
    21721 cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg agatctcttt
    21781 tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc acggcgaact
    21841 gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc aactcaattt
    21901 tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc
    21961 actgatggca aaacactacc atcgcctgaa acctgtaaaa gattatcagg agattgacga
    22021 cgtactgttc aagttttcgc tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa
    22081 actacacata gcgctggatg gcattatgaa acccgtcacc agtggttttg gctttatcga
    22141 cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga aacgctggga
    22201 tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg agatgctgaa
    22261 aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg
    22321 ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga
    22381 taacacatcc ccttttaaca gctgacctct ctaccggagc catgcgctcc ggttttccct
    22441 tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt atttataact
    22501 tgtattgtca catgcaaatc agcatagttt cgaatgaaag ttaatgcgag gtggttatgg
    22561 cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc cgtcagatga
    22621 agcacgcgct gcgggcgcag cttggcgatg tccagcagat ctttaatcag ctaagcgatg
    22681 acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat gccgtctggc
    22741 cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag cgcgaacaga
    22801 ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa gcgctaggct
    22861 gggatcagtc gatgctggac tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc
    22921 ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac cccatctact
    22981 ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc
    23041 tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg
    23101 tcatctaccc tgaccgtatc cgccgccttc cgcccggaac gacctccaaa ggtcttggag
    23161 cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag cacgttttcg
    23221 ccaacgtctt taatggcaat ctggcgaaat acgatccctg gcatgcggca catcgtacgg
    23281 aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg acattccagg
    23341 gctggactgc gctctcggat atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc
    23401 ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac
    23461 tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat ccactgttaa
    23521 ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg tggcactgcg
    23581 acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca
    23641 ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac
    23701 tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag
    23761 gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttgggc atggatgttt
    23821 gagagttaat ggaagtgaat tgatgtggga ttaagaaaat cgattcgacg ttactcagat
    23881 tgctgacaag tgcgctttct taatgcctga tgcggcgtta acgccttatc cggcctacaa
    23941 agttctgcag attcaatata ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag
    24001 gctattttgc atttgtcatc agccgtcgtc accccgacag tatcggggtg acggataatc
    24061 acttactcga tccctttact acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg
    24121 cgttccgggg tgatttccag aatgcgggtc gccagggagc ttacgaactc acggtcgtgt
    24181 gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca tgttcagtga ctcgatggat
    24241 tccatatcca ggtggttggt cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt
    24301 aacttaccaa acagcatccg ccctttttcc ccaccggaaa gcactttagc tggctttttg
    24361 atgtcgtcct ggctgaacag caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg
    24421 ccttcctgct tccactgact catccattcg aacacggtca gatcattttc aaactcatat
    24481 tcgtggtcct gggcgtagta accaatgcgc gcgttttcag accattttac ggtgccgctg
    24541 tccggttgca gatcgcccac cagcgttttc agcagcgttg atttaccgac gccgttggta
    24601 cccagtaccg caagtttttc accgacctcc agcagcaggt tgagattttt aaacagtgga
    24661 ccgttatcaa accctttggt cagaccttcc acttccagcg cgttacggaa cagtttctta
    24721 tcctgttcaa aacggatgaa cgggttctga cggctggagg ctttcacctc ttccagtttg
    24781 attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta
    24841 aagcggctaa cgaaagattg caactcagca atctgcgctt tcttcttggc gttatcggcc
    24901 agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt acccggataa
    24961 acgcgcagct cgccgtaatc cagatccgcc atgtgggtac agaccatgtt aaggaagtga
    25021 cggtcgtgcg agatgatgat catggtgctg tcacgctcgt tcagcacctg ttccagccag
    25081 cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat atccggctca
    25141 gcaaacagcg cctgcgccag aagcacacgc agcttccagc caggagcaac ttcactcatc
    25201 gggccgtagt gctgttccac tggaattccc acgccaagca acagttcacc ggcgcgagct
    25261 tccgcagagt aaccgtccat ttcgccgtat ttcacttcca gatcggccac tttatagccg
    25321 tcttcttcac tcatttccgg caaagcatag atgcggtcgc gctcctgctt cacttcccac
    25381 aactctttat gccccatgat caccgtatcc agcacagtga acccttcaaa ggcaaactga
    25441 tcctgacgca gcttaccaat gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc
    25501 tcaaggtcgc cgccgagaat cttcataaag gtggatttac cactaccgtt cgcgccaatc
    25561 aggccgtaac ggttgccgcc gccgaatttg acggaaatgt tttcaaacaa cggcttactg
    25621 ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa agagatatga
    25681 caaaccgcgc attatgccat aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca
    25741 ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt gagctggaat
    25801 gaacttataa tgcgcttcca atactctaat attctcaacc caatggcctg ccaggcacaa
    25861 aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct tcgctgttgt
    25921 cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct
    25981 ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc tggagtattt
    26041 tgccgcggag taccagatgg gactttccaa tatgatggaa gcgaacccgg gtgtggatac
    26101 cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc tgccggatac
    26161 cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact atccgaaagg
    26221 gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag atacgcctat
    26281 caactggacc accaaagttg agcataagaa agcaggcccg acctggacgc cgaccgccaa
    26341 aatgcacgca gagtaccgcg ctgcgggcga accgctaccg gctgtcgttc ctgcaggtcc
    26401 ggataacccg atgggtctgt atgcactcta catcggtcgc ctgtatgcta tccatggcac
    26461 caacgccaac ttcggtatcg gcctgcgtgt aagtcatggt tgtgtgcgtc tacgtaacga
    26521 agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt ttattgatga
    26581 gccggtaaaa gcgaccaccg agccagacgg cagccgttat attgaagtcc ataacccgct
    26641 gtctaccacc gaagcccagt ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag
    26701 cgtgcagaca gtgaccggtc agccagatgt tgaccaggtt gttcttgatg aagcgatcaa
    26761 aaatcgttcc gggatgccgg ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga
    26821 ctcataatcc gcctttttta tttgccagac cttaccaggc cgggatcata actaacaaaa
    26881 cgtatcccac cagcgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc
    26941 agatgcggcg atcgttcgcc atacgtagcg caatcaaatt tgccagcgat cctggtaata
    27001 aaccaaagcc ccccacgttt accgcccatg ccagtaataa agacggaggt acataattga
    27061 gcagcaaaat ggtactcggc acgttactga tcacctgcga taaaccgact gccgttaacc
    27121 ataacccggg ttcagataaa tgactcacgt tacccaacac gccttgcaac gctggaagct
    27181 gggtcagtaa atggacgtcg ataaacatcg ccataaacac cagcagcaac gtccagtcca
    27241 cactcaacac cacgcgacgc gccagcagca caaagcccgc cgccacaatc accagtcccc
    27301 acagctcttg tttgaactcc agcgccgtca gaaaaacgat atacagcccc agacaattcc
    27361 acaccagccg cggtttccac tccggtgttt gcatccccgt atggtattgc agcgcctttc
    27421 cagggaaaca acaccagcac aacagcagga gtgtcagcat cattgcgcca gccagcggtg
    27481 ccatttgggc aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt
    27541 gcgggttgcc aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa
    27601 tcagccgatt aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa
    27661 taaacagcgc gacatcgttg gtctgaaagg tagaaagcag cgccgccgcc agcaccataa
    27721 acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa
    27781 aataaccgct taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg
    27841 tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc
    27901 cgataccaac aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag
    27961 gcaggctcat ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc
    28021 actaacatgg tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa
    28081 caccaacaag aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc
    28141 tccttctgcc gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc
    28201 cagccgctta agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc
    28261 cacctgacga gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc
    28321 aataagctcg cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac
    28381 gttcactaac tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct
    28441 tcctcattat ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag
    28501 ttgcgaaggt acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata
    28561 tctgtatgta atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta
    28621 tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa
    28681 tgctgctctc tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttcag
    28741 ataaatagcg gcgtcgcggt acgccgcttc actcctgctt ttatgcaggc ataacgcgtt
    28801 ttggtctgaa aaaaaccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc
    28861 gtcagtagtt gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg
    28921 gctcgcggat gcggacccct ttcccctctt cacgcactct tgcaggtatt gacccttgac
    28981 gccagggtaa gcacatggcg tttgttacca tagtggcata tgaatttaac cctcaaagaa
    29041 tcgcttgtta cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg
    29101 ctattaatta acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg
    29161 gctattttgc ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc
    29221 agttcgctgg tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc
    29281 aatacattgc tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt
    29341 tttccgtggt acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc
    29401 aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta
    29461 ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt
    29521 aaactgaaag atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat
    29581 aacgaggtga ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg
    29641 acggtcactg ccgttaagcc gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg
    29701 cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc
    29761 gttaacggtt tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt
    29821 ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc
    29881 ctagcaaatc gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa
    29941 tacgatactg ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa
    30001 ggtaattttg aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa
    30061 gtgctggcgc aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat
    30121 ccgcaggcct gcgacaggac acaaggcaaa tacaaaacct ttgtgcaatc gaaagaaacg
    30181 tcgtgctatc tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag
    30241 ttacgcaaca gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt
    30301 aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc
    30361 caggtgcctt ttatggtcat ttccagcggc gataaagcgc atcgggtgat taaagcccgc
    30421 cgctcagcca atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa
    30481 ataaatatca aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac
    30541 ttccagttac agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct
    30601 taaaaacaaa aatccgcccc gagaggcgga tttttatatc accaaagtga ttagaagcgg
    30661 taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa
    30721 gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc gtaggagaaa
    30781 ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa tttaccataa
    30841 cccacaccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca
    30901 gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg gcttttctcg
    30961 gtgtaagtga aagaaccgat cacacccagc gggctgttgt cttcttcata gcggtatttc
    31021 aggtttaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca
    31081 gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac tgcggccagt
    31141 gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat aagtcctaag
    31201 ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca
    31261 tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat cactttttca
    31321 gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta ttcctaatca
    31381 ccagactaat gattccatca atcctggcgc attttagtca aaacggggga aaattttttc
    31441 aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac ttaagtctag
    31501 atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg gttaccaata
    31561 gtcatattac tcgttgccat ggcgtctatt cagggtggag cctcgttagc taagtcactt
    31621 tttcctctgg tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg aacgctgatc
    31681 ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg gttaccgctg
    31741 ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctttc tattcagaca
    31801 gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt ggcgctgttc
    31861 tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc
    31921 ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc gctggcactg
    31981 ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg agcggaacat
    32041 ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt gccaattgga
    32101 gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg tctggctgtc
    32161 gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac ccgtttgcca
    32221 acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt ttccgggatg
    32281 attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc tatcatcgcc
    32341 gcttcaatgg ggtctacgct gacagtacgc aaagagagca aaataaaaga attagacatt
    32401 aattaaattt acatttctgt atggttatgc ataaccatgc agaatttctc gctacttttc
    32461 ctctacaccg tctttatata tcgaattatg caaaagcata tttattccga aaattcctag
    32521 cgagcagata aataagaatt gttcttatca atatatctaa ctcattgaat ctttattagt
    32581 tttgtttttc acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca
    32641 catagccggt gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga
    32701 aattatgagt accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa
    32761 cgatgtctcc gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca
    32821 gtttattgat ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt
    32881 cattgccgta catgaaatgc tggatggctt ccgcaccgca ctgatcgatc atctggatac
    32941 catggcagaa cgtgccgtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa
    33001 cagcaaaacc ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa
    33061 agaactggct gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc
    33121 gaaagatgac gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct
    33181 gtggtttatc gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ctttagtacc
    33241 cgataaaagc ggcttcctga caggaggccg ttttgttttg cagcccacct caacgcactt
    33301 atttagtgca tccatctgct atccccagct gattaagtaa attttttgta tccacatcat
    33361 cacacaaccg ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg
    33421 ttgagtgcac aattttagcg caccagattg gtgccccaga atggtgcatc ttcagggtat
    33481 tgctctataa atcgtgcatc acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa
    33541 ctggcacgat tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa
    33601 aaggaaatgc tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg
    33661 cggtttcttc tcatgccgcg gataaaaaat tagttgtcgc gacggatacc gccttcgttc
    33721 cgtttgaatt taaacagggc gataaatatg tgggctttga cgttgatctg tgggctgcca
    33781 tcgctaaaga gctgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc
    33841 cggcactgca aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc
    33901 gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga
    33961 aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga
    34021 agagcggtac tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc
    34081 agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc
    34141 tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag
    34201 cggtaggtga ctctctggaa gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg
    34261 agctgcgtga caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg
    34321 aagtctacaa aaaatggttc agtactgaac cgaaataata acgctacacc tgtaaagcgc
    34381 actggcagtt ccctctcccc cctggggaga ggattagggt gaggggcgca aacccgctcc
    34441 ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc
    34501 agtttgactg gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga
    34561 ccctgtggat ttcggtcctc ggtctggctg gcggtctggt aatcggattg ctggcaggtt
    34621 ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga
    34681 tccgcggcac acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt
    34741 ttaacgactt acgcatcgac ccatttactg cggcggtggt caccatcatg atcaactccg
    34801 gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg
    34861 aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc
    34921 tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca
    34981 cctcactgtt tattgttatc ggcgtggcgg aactgacccg tcaggggcaa gaaattattg
    35041 ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta
    35101 ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga
    35161 atttaaaaac gtctccaagc actttggctc aacccaggtg ctgcacaata tcgatttgaa
    35221 cattgcccag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct
    35281 gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct
    35341 gaaggttaac gatccgaaag ttgacgagcg cctgattcgc caggaagcag gtatggtgtt
    35401 ccagcagttt tacctcttcc cgcatctgac ggcgctggga aacgtcatgt ttggtccgct
    35461 acgcgtgcgt ggcgcgaaca aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa
    35521 agtcggtctg gcagaacgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca
    35581 gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc
    35641 gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc
    35701 tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc
    35761 ttcgcggctg atctttatcg acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt
    35821 gatcaagaac ccgccgagcc agcgcttgca ggaatttttg cagcacgtct cttaataaga
    35881 cacattgcct gatgcgctac gcttatcagg cctacaggat atctggcaac ttattaaaat
    35941 tgcatgaact tgtaggacgg ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg
    36001 ttgtcagcaa ccggcttaat atcccttcct ccctttcacc cgaaagggag gcacaccaga
    36061 ttcctctcat ttaaaatcgc ccctccccca gcatctatac ttatcttttt gctctgtttt
    36121 ctcactggag gagtcatgcg gtggatcctg ttcatcctct tctgcctgct gggcgcacct
    36181 gcccacgcgg tatccatacc cggcgttaca accacaacga caacggactc aacgactgaa
    36241 ctggccccgg aaccggatat cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc
    36301 tggataacga cacctcgcgt aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc
    36361 cccctgcgga accggtcccg aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc
    36421 tgcaaaaggt caccgaagtc agccgccatt atggtgaagc cctttccgcc cgcttcgggc
    36481 aactttatcg caatatcacc ggctccccgc ataagccatt taatccacaa accttcagca
    36541 atgcgctgag ccatttttca atgttagcgg tattagtgtt tggtttttac tggctgattc
    36601 gcctgtgcgc actgccgcta tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg
    36661 agcgcagcaa ctggttgcag cttccggcga tgattattgg ggcgtttatt atcgacctgc
    36721 tgttactggc cctgacatta tttgtcggcc aggtattaag cgacaacctg aatgcaggta
    36781 gtcgcaccat cgctttccaa caaagtttgt ttctcaacgc ctttgccctg attgaatttt
    36841 tcaaagccgt actacgcctg attttttgcc caaacgtggc ggagctgcgc ccgttcacga
    36901 ttcaggacga gagcgcccgt tactggagtc gccgcctgag ctggttaagc agcctgattg
    36961 gctatggcct gattgtggcc gtgccgatta tctctaatca ggtgaatgta cagataggtg
    37021 cgctggcgaa cgtcatcatt atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc
    37081 gaaataaaaa agagattacc cagcatttgc tcaactttgc ggagcattcg ctggcttttt
    37141 tcagcctgtt tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc gcctatttta
    37201 tcgtgctgtt tttcttttcg ttgttcgatc cgggcaacag tctgaaattt atgatgggtg
    37261 caacggtgcg cagcctggcg attattggta tcgcagcgtt tgtttccggt atgttttccc
    37321 gctggctggc gaaaaccatc accctctcgc cacatactca gcgtaactat ccggagctac
    37381 aaaaacggct gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct
    37441 gcgtggcagt aatgttgctg ttgagcgcat ggggattgtt cgatttctgg aactggctgc
    37501 aaaacggcgc ggggcagaaa accgtagata tcctgatccg catcgcactc attcttttct
    37561 tctcggcggt tggctggacg gtgctcgcca gtttgatcga aaaccggctg gcttcggata
    37621 ttcatggccg cccgctaccc agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg
    37681 cgctggcggt gattatcagt accatcacca tcatgattgt gttgtcggaa atcggcgtca
    37741 atatcgcgcc attgctggca ggtgccgggg cattaggtct ggctatctcg tttggttcgc
    37801 aaacgctggt aaaagatatt atcaccgggg tatttattca gtttgaaaac ggcatgaaca
    37861 ctggagattt ggtgactatc gggccgttga ccggcacagt ggaacggatg tcgattcgct
    37921 ccgtgggcgt gcgccaggat accggggcgt atcacatcat tccgtggtct tcgataacca
    37981 cctttgctaa cttcgtccgc ggtattggtt cggtagtggc aaactacgat gttgatcgcc
    38041 atgaagatgc tgataaagcc aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa
    38101 acgaagaaat tcgcgggctg attattggtg aaccgaactt tgccgggatt gtcggcttaa
    38161 gcaataccgc gtttacactg cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg
    38221 tacgctttgc cctcgacagc caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc
    38281 cagtgcagac ttatcaggtg ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc
    38341 cgggggagcc aacgctttaa cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca
    38401 taaaggtcca ggcaataaag cgactctgct tttgcccctg ggccatctct ttttttacca
    38461 ccttcaccgc gcccacgtcg gtcagagcac gatacaacgg cggtaagttt tcaccacgag
    38521 ataccagcga ggtaaaccac atcacctgct tcgcgaagcc tttgctctct tcaatcatct
    38581 ttttgataaa gttgacttca ccgccttcac accacaactc ctgttgctga ccaccaaagt
    38641 tcagtgcatc gtctttgttc agccccaggt tacggcgttt acgctcacta cctgcccgcg
    38701 ccgcagcggc ggaatcgtgg aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt
    38761 tgtggatgat gccgttaaaa atcgccccac tctctttttg ccgacgcaga cgaatggcgc
    38821 ggttaagccc cggattagca ctgataatcg cctgcgcact gcttaacgcc tggctgctgg
    38881 tttcactacc ggtaaaacgc cagccatatt catgtacgcc aatcagcgga taaatacagt
    38941 tcgcaccaac gccgatatcc agaatgctgg cattcgctgg aattgttccg cttgcttcag
    39001 ccagtaaatc ggcaaggtga tgaatataat ccgcccggcc cggtactggt gggcagagaa
    39061 aaccgtcggg gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac gccttattga
    39121 gcgccttcac cgccagcgga ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg
    39181 tgaggaattg cctgagttca ggattgacct gacaaagcgt ggcgagatcg tagcggctgt
    39241 gatgacggtt gcgcggatgc aaccccggtt tctgggcgga catagcattc tcctttaagc
    39301 agcggcgtaa gatacccgca taacgcgaca gggtaaatat ctggctgcgt ttatcattcc
    39361 ccaaaacctt aacatggcta tggtttatgg caattcatca taaaaatcag cattatgcat
    39421 gtcattgatt tgtattattt tgtaaaaata tatcgttcaa aaaataagca tatctctgtc
    39481 aattttttgc gtcatttgca tcgcgcaggc gacctacact tatcaggcac tacccgttgt
    39541 atttatgagg atgtcattat gaaaaagtgc ctcacactac tgattgccac cgtcctgagc
    39601 ggaatctctc tcacggctta tgccgcgcaa ccgatgagta acctggacag cggtcagcta
    39661 cggcccgccg gtaccgtttc ggcaactggc gcatcaaacc taagcgatct ggaggataaa
    39721 ctggcagaaa aagcgcgcga acaaggcgcg aaaggttatg tcattaattc cgcaggcgga
    39781 aatgaccaga tgttcggtac tgcaaccatc tacaaataac cccaccaacg taactgcatt
    39841 cgcctctgga tgcagttttc gcctgttttg taatcaaaaa acatcaaata tgaacgcaat
    39901 gtaaataaat gtatttcttt ttcgcgcaat gggtgataga aaatcgcgtc aagtgataat
    39961 acttatcaaa attattatca cttgcacaaa cactatcacg ggattaacag tggcatcgca
    40021 tccgcagaga tgctttctcg tggcagtgaa aatttcaaca tataagaaaa agtcacctgc
    40081 aaaatggaaa acaatcgcaa tttccctgcc agacaatttc attcgctcac gttctttgcc
    40141 ggtctttgta ttggtattac gcctgtggca caggcactcg ccgccgaagg gcaagctaac
    40201 gcggatgaca cgctggttgt cgaagcatca acgccttcgc tttatgcgcc acaacaatct
    40261 gccgatccga aattctcgcg tccggtagcg gatactaccc gcacgatgac ggtgatttct
    40321 gaacaagtga ttaaagatca gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc
    40381 ggcg
//
LOCUS       DADWTH010000027        39557 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.027, whole genome shotgun sequence.
ACCESSION   DADWTH010000027 DADWTH010000000
VERSION     DADWTH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.027"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..189)
                     /locus_tag="JNY02_001502"
     CDS             complement(<1..189)
                     /locus_tag="JNY02_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBB9433094.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(330..2363)
                     /gene="metG"
                     /locus_tag="JNY02_001503"
     CDS             complement(330..2363)
                     /gene="metG"
                     /locus_tag="JNY02_001503"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBB9433095.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2495..3604
                     /gene="apbC"
                     /locus_tag="JNY02_001504"
     CDS             2495..3604
                     /gene="apbC"
                     /locus_tag="JNY02_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBB9433096.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3866..4147
                     /locus_tag="JNY02_001505"
     CDS             3866..4147
                     /locus_tag="JNY02_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBB9433097.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4440..4982
                     /locus_tag="JNY02_001506"
     CDS             4440..4982
                     /locus_tag="JNY02_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9433098.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5063..5737
                     /locus_tag="JNY02_001507"
     CDS             5063..5737
                     /locus_tag="JNY02_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBB9433099.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5753..8233
                     /locus_tag="JNY02_001508"
     CDS             5753..8233
                     /locus_tag="JNY02_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBB9433100.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPLVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8249..9282
                     /locus_tag="JNY02_001509"
                     /pseudo
     CDS             8249..9282
                     /locus_tag="JNY02_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9364..9702)
                     /gene="rcnB"
                     /locus_tag="JNY02_001510"
     CDS             complement(9364..9702)
                     /gene="rcnB"
                     /locus_tag="JNY02_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBB9433101.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9921..10769)
                     /gene="rcnA"
                     /locus_tag="JNY02_001511"
     CDS             complement(9921..10769)
                     /gene="rcnA"
                     /locus_tag="JNY02_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBB9433102.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            10890..11162
                     /gene="rcnR"
                     /locus_tag="JNY02_001512"
     CDS             10890..11162
                     /gene="rcnR"
                     /locus_tag="JNY02_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBB9433103.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11254..11350
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11385..12173
                     /gene="thiM"
                     /locus_tag="JNY02_001513"
     CDS             11385..12173
                     /gene="thiM"
                     /locus_tag="JNY02_001513"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBB9433104.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12170..12970
                     /gene="thiD"
                     /locus_tag="JNY02_001514"
     CDS             12170..12970
                     /gene="thiD"
                     /locus_tag="JNY02_001514"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBB9433105.1"
                     /translation="MKRINALTIAGTDPSGGAGILADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13035..13853
                     /locus_tag="JNY02_001515"
     CDS             13035..13853
                     /locus_tag="JNY02_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBB9433106.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13905..14651
                     /locus_tag="JNY02_001516"
     CDS             13905..14651
                     /locus_tag="JNY02_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB9433107.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14625..15590)
                     /locus_tag="JNY02_001517"
     CDS             complement(14625..15590)
                     /locus_tag="JNY02_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBB9433108.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15587..16590)
                     /locus_tag="JNY02_001518"
                     /pseudo
     CDS             complement(15587..16590)
                     /locus_tag="JNY02_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16587..17063)
                     /locus_tag="JNY02_001519"
     CDS             complement(16587..17063)
                     /locus_tag="JNY02_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9433109.1"
                     /translation="MLARSSFHRKGIQKVLLCGIVIAYLTLPVNFTYALLFLGILLHG
                     VSYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQE
                     PVNGLTFNWSGMWTFGAVMIAIIAVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            17217..18269
                     /gene="fbaB"
                     /locus_tag="JNY02_001520"
     CDS             17217..18269
                     /gene="fbaB"
                     /locus_tag="JNY02_001520"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBB9433110.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18577..19431
                     /gene="gatY"
                     /locus_tag="JNY02_001521"
     CDS             18577..19431
                     /gene="gatY"
                     /locus_tag="JNY02_001521"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBB9433111.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRLKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNLAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19460..20722
                     /gene="gatZ"
                     /locus_tag="JNY02_001522"
     CDS             19460..20722
                     /gene="gatZ"
                     /locus_tag="JNY02_001522"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBB9433112.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAVVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            20732..21184
                     /gene="gatA"
                     /locus_tag="JNY02_001523"
     CDS             20732..21184
                     /gene="gatA"
                     /locus_tag="JNY02_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBB9433113.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21215..21499
                     /gene="gatB"
                     /locus_tag="JNY02_001524"
     CDS             21215..21499
                     /gene="gatB"
                     /locus_tag="JNY02_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBB9433114.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21503..22859
                     /locus_tag="JNY02_001525"
                     /pseudo
     CDS             21503..22859
                     /locus_tag="JNY02_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            22907..23947
                     /gene="gatD"
                     /locus_tag="JNY02_001526"
     CDS             22907..23947
                     /gene="gatD"
                     /locus_tag="JNY02_001526"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBB9433115.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24053..24826
                     /locus_tag="JNY02_001527"
     CDS             24053..24826
                     /locus_tag="JNY02_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB9433116.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQEAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(24908..25807)
                     /gene="yegS"
                     /locus_tag="JNY02_001528"
     CDS             complement(24908..25807)
                     /gene="yegS"
                     /locus_tag="JNY02_001528"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBB9433117.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            26213..26530
                     /locus_tag="JNY02_001529"
     CDS             26213..26530
                     /locus_tag="JNY02_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433118.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(26872..28233)
                     /gene="yegQ"
                     /locus_tag="JNY02_001530"
     CDS             complement(26872..28233)
                     /gene="yegQ"
                     /locus_tag="JNY02_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBB9433119.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28380..28702)
                     /locus_tag="JNY02_001531"
                     /pseudo
     CDS             complement(28380..28702)
                     /locus_tag="JNY02_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(28893..29615)
                     /gene="baeR"
                     /locus_tag="JNY02_001532"
     CDS             complement(28893..29615)
                     /gene="baeR"
                     /locus_tag="JNY02_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBB9433120.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHESGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(29612..31015)
                     /gene="baeS"
                     /locus_tag="JNY02_001533"
     CDS             complement(29612..31015)
                     /gene="baeS"
                     /locus_tag="JNY02_001533"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBB9433121.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(31012..32427)
                     /locus_tag="JNY02_001534"
     CDS             complement(31012..32427)
                     /locus_tag="JNY02_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9433122.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(32428..35493)
                     /gene="mdtC"
                     /locus_tag="JNY02_001535"
                     /pseudo
     CDS             complement(32428..35493)
                     /gene="mdtC"
                     /locus_tag="JNY02_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
     gene            complement(35494..38616)
                     /gene="mdtB"
                     /locus_tag="JNY02_001536"
     CDS             complement(35494..38616)
                     /gene="mdtB"
                     /locus_tag="JNY02_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBB9433123.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAEIIGYRALPVS
                     ALPEVDYPTIQVVTLYPSASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            complement(38616..>39557)
                     /locus_tag="JNY02_001537"
     CDS             complement(38616..>39557)
                     /locus_tag="JNY02_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HBB9433124.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
BASE COUNT         9918 a         9685 c        10238 g         9716 t
ORIGIN      
        1 ccccttcttc accttctccg caatcagctt cagttccgct ttttccgccg ccgcagcatc
       61 tgaaaaactt tttatctgac tttgcccttt ggtgccaact tttccccaac tgatatgcaa
      121 ctcgtttccc tgttgctcca ccgtccagaa tttatgtgat ttttcgtcct gatagataaa
      181 gtgtctcatt ctgcattcct gtaattttat ccagttgaat gcagatgcta ccagtattta
      241 tacgggttag agagagacaa aatcgcaaga aaacaacttt aggaatttat aaagaaaagg
      301 cgctgtcgat gcagcgcctt gaagggggat tatttcacct gatgacccgg tttagcaccg
      361 gcatccgggc ttagcaggaa aatatctttc ccgccaggac cggcagccat caccatgcct
      421 tcagagatac cgaagcgcat tttacgtggt gccaggttag ccaccataat ggtgtgacga
      481 ccaatcagtg cctgcggatc cgggtaagca gaacgaatac cggagaagac attgcgtttt
      541 tcaccgccga gatccagcgt caggcgcagc agtttgtcag aaccttcaac aaactctgcg
      601 ttttcgatca gcgccacgcg caggtcaact ttggcgaagt cgtcaaaggt gatggtttcc
      661 tgaatcggat catctgccag tgggccagta actggcgcgg tagtggcttt cacttcttct
      721 ttagaggctt ccaccagagc ttcaacctgt ttcatatcga tgcggttata cagcgccttg
      781 aacggattca ctttgtggcc cagcagcggt tgctggatac catcccaggt cagttccgta
      841 ttgaggaagg cttctgcacg ctcggtcagt ttcggcagta ccggcttcag gtaagtcatc
      901 agcacgcgga acaggttgat gcccattgag caaatcgcct gcaggtcagc atcgcgacct
      961 tcctgttttg cgactaccca cggagcctgc tcgtcgacat agcggttagc caggtcagcc
     1021 agtgccatga tttcgcgcac tgctttacca aattcacggc tttcccacgc ttcaccaatc
     1081 acttcagcgg catcggtaaa ggttttgtac agctcagggt cagccagctc gcttgccagc
     1141 acaccgtcaa aacgcttgtt gataaagccc gcattacggg aggccaggtt aaccactttg
     1201 ttcacgatat cggcattcac acgctgaacg aaatcttcca ggttgagatc gatatcatca
     1261 atgcgcgaag agagtttcgc agtgtagtag taacgcaggc tgtcagcgtc aaaatgattc
     1321 agccaggtgc tggctttaat aaaggtgccg cgagacttgg acatcttcgc gccgttcacc
     1381 gtcacatagc catgaacaaa caggttggtc ggcttgcgga agttgctgcc ttccagcatg
     1441 gcaggccaga acaggctgtg gaagtaaacg atatctttac cgatgaagtg gtacagctcg
     1501 gcggtggagt ctttcttcca gtatttatcg aagcttacgc tgtcgccgcg cttgtcgcac
     1561 agattcttga aagaacccat gtagccaatc ggtgcgtcca gccagacgta gaaatatttg
     1621 cccggcgcgt tcggaatttc aaaaccaaag tatggcgcgt cgcgggagat atcccactgt
     1681 tgcaggccgg attcaaacca ctcctgcatt ttgtttgcca cctgctcctg caacgcaccg
     1741 ctgcgggtcc atgcctgcag catttcgctg aaagagggca gatcaaagaa gaagtgttca
     1801 gaatcacgca tcactggtgt agcaccagaa accaccgatt tcggctcgat caattcagtc
     1861 gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt attgatccgg ggatttacat
     1921 ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca tgcctttttc cggatcgtac
     1981 agctgagaga tggtgcggtt tttaataaaa ccgttttctt tcaggcgaga gtagataagt
     2041 tctgacaact ggcggttctc ttcgctgtgc gtcgagtgat agttgtcata gctgatgtta
     2101 aagcctgcga aatcagtctg atgctcctga ctcatttcgc caatcatctg ctccggggtg
     2161 ataccgagct gctgagcttt cagcatgatc ggtgtaccgt gggcatcgtc ggcgcagatg
     2221 aagttgacct cgtggccgcg cattcgctgg taacggaccc agacatcagc ctggatgtgc
     2281 tccagcatat ggccgaggtg gattgagccg ttagcgtacg gcagtgcgca cgtcaccaga
     2341 attttcttcg cgacttgagt catagtaggc attacttctt aatagtgaaa aggggttaga
     2401 tagtaccaaa tgggaaaatg ttaagtaagt acttctctat attcttcagg taaactatca
     2461 gctgatggtt tatctataca aaaaaggagt cgggatgaac gaacaatccc aggccaaatc
     2521 accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc aattttcagc acccaaccct
     2581 gaagcataac ctcaccacgc tgaaagcgtt gcaccatgtc gcctggatgg acgacacgtt
     2641 gcatgtggaa ctggttatgc cgtttgtctg gcatagcgcg ttcgaagagt taaaagagca
     2701 atgtagcgcc gaactgctgc gcatcaccgg ggcgaaagct atcgactgga aactgtcgca
     2761 taacatagcc actctgaaac gcgtgaagaa tcagccggga attaacggtg tgaagaacat
     2821 tatcgcggtg agttccggta aaggcggcgt gggtaaatcg tccacggcgg taaacctggc
     2881 gctggcactg gcggcggaag gggcgaaagt cggcattctg gacgccgata tctacggccc
     2941 atcaattcct accatgctgg gcgcagaaaa tcagcgtcca acttcgccgg acggtaccca
     3001 catggcaccg attatgtctc atggcctggc aaccaattca attggttatc tggtcactga
     3061 cgacaatgcg atggtgtggc gtggaccgat ggcgagcaag gcgttaatgc agatgttgca
     3121 ggaaaccttg tggccggatc tcgactatct ggtgcttgat atgccgccag gcaccggtga
     3181 cattcaattg acgctggcgc aaaacattcc agtaactggt gcagtcgtgg tcaccacccc
     3241 gcaggacatc gcgttgatcg atgcgaaaaa aggcattgtg atgttcgaga aagtcgaggt
     3301 tccggtgctc ggtattgtcg agaacatgag tgtgcatatt tgcagtaact gtggtcacca
     3361 tgaaccgatt ttcggtaccg gtggtgcaga gaaactggct gaaaaatacc atacacagct
     3421 gctggggcag atgccgttac acatctcctt acgtgaggat ctcgataaag gcacgccaac
     3481 ggttatcagc cgtccggaga gcgaatttac cgctatctac cgccagctgg ctgaccgcgt
     3541 tgcagctcag ctctactggc agggtgaagt cattcctggc gagatttcct tccgcgcggt
     3601 ctaacgccta ttaaccattc tggttattct tcatacaccg cagagatgtt atattgatgc
     3661 ggtgtatttg tttttgccgc attaatgaac acttatttaa tactgcgaat attctgcgga
     3721 ccttcgtccg aaactctaca aagtcgagtg atcgtacagg caatgcttca ttatcatcct
     3781 tcgtgctgtg tagtggatgt acagctatag tttgttatgc actcattcta tccaggcata
     3841 acctttacta cagggataac gttcaatgaa taagtattgg ctgtcaggaa ttatttttct
     3901 tgcctatgga ttggcttctc ccgcagtctc ttcagagact gccacattaa cgattaatgg
     3961 caggatcagt cccccaacat gcagtatggc tatggttaat agtcaaccac aacagcattg
     4021 tggtcagtta acgtataatg ttgacacccg tcatcaggtt tcttcgcccg tcaaaggtgt
     4081 tacgacagaa gtggttgttg caggtagcga tagcaaacgt cgaatcgtgt taaatcgcta
     4141 cgattaattt ggcaacctga ttttaattgc atttagaaag acgaaatcat ttatataaat
     4201 gatttcgtct tttttattga tatcaacaat accatttaca taaatctaca ttaattaata
     4261 gatgtgataa atatttaata ttcttaagta tttgttgtat ttatattgca ttggttaatt
     4321 tgttttaaat caataaaaca atatgtttac attgcggcag cttttttgat gtgagaaatt
     4381 tgtttccaag agatacagac tcttaacaac caaaaataaa ttttattaag gaacatatta
     4441 tgaaacgttc aattattgct gccgctgtct tttcttcttt ttttatgagc gctggagtat
     4501 ttgctgcaga cgttgatacc ggaacattaa ccattaaagg aaatattgca gaatctccgt
     4561 gtaaattcga agcgggtggt gattcagtaa gtattaatat gccgactgta ccaaccactg
     4621 tctttgaagg taaagctaaa tattctacct atgatgatgc agtcggtgta accagcagca
     4681 tgttaaaaat tagctgcccg aaagaagttg ctggtgtaaa actctcgttg atcacaaacg
     4741 acaaaataac cggtaacgat aaggcgatag ccagtagcaa cgataccgtg ggttactatc
     4801 tctatttagg tgataacagc gatgtcctag atgtttctgc accttttaac attgagagtt
     4861 ataaaacagc agaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag
     4921 ataactcagt gcaatcaggt gatgtgttat cttctctggt tatgcgtgta gcgcaggatt
     4981 aataaataaa attgcaagtt tctccaggta ataaacatgg ccgctatccc catggcggct
     5041 ttttaattta agaggcatta agatgaaagg attattatct ttactcattt tttctatggt
     5101 ccttcctgca catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa
     5161 taaagaagtt atggtgcagt tgatgaacca gggaaatcgt tcttcgctgc tgcaggcgtg
     5221 gattgatgat ggcgatacgt cgttaccacc agaaaaaatt caggttcctt tcatgttaac
     5281 gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc
     5341 gaataaactg cccactaata aagaaagcat tttttatctg aatgttctgg atattccacc
     5401 aaatagtcca gagcaagaag gcaagaatgc actgaagttt gcgatgcaaa acagaattaa
     5461 gttgttttac cggccagcgg gtattgctcc ggtaaataaa gcgaccttta aaaagttact
     5521 ggtaaatcgc agtggcaatg gattggtgat aaaaaatgac tcagctaatt gggtgacgat
     5581 ttcggatgtc aaagctaata atgttaaagt caattatgaa actattatga ttgccccctt
     5641 agaaagtcag agtgttaata tcaaaagtaa taatgcaaat aactggtatc tgaccattat
     5701 caatgaccac ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
     5761 aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc
     5821 tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa
     5881 tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa
     5941 taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt
     6001 atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca
     6061 atgtttaaca tttgagcaac ttgttcaggg tgggagctat agctgggata tcggggtttt
     6121 tcgtctcgat ttcagtgtcc cgcaggcttg ggtggaagaa ctggaaagtg gctatgttcc
     6181 accggaaaac tgggagcggg gtattaatgc gttttatact tcttattatg tgagtcagta
     6241 ttacagcgac tataaagcgt cgggtaacaa caagagtaca tatgtacgtt ttaacagcgg
     6301 gttaaattta ctggagtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa
     6361 tccaggggtg tggaaaagca ataccctgta tctggaacgt ggatttgccc aatttctcgg
     6421 cacgcttcgc gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag
     6481 cggtgtgcgg ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa attttacgcc
     6541 acgggtgcag gggattgctc agagtaacgc gctggtaact attgaacaga atggttttgt
     6601 ggtttatcag aaagaggttc ctcctggccc gtttgcgatt acagatttgc agttggccgg
     6661 tggtggagca gatcttgatg ttagcgtgaa agaggcggac ggctcggtaa ccacctatct
     6721 ggtgccttat gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat atgattttgc
     6781 ggcgggccgt agccatattg aaggggcgag caagcaaagt gattttgtcc aggcaggtta
     6841 tcagtatggt tttaataact tattgacgct gtatggcggc acgatggttg ctaataatta
     6901 ctatgcgttt accctcggaa cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc
     6961 cacgaagtcg catagtaaac aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat
     7021 tgcctacaac aaatttgtga gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta
     7081 ttcgtcgcgt gattaccgga catttaacga tcacgtttgg gcaaacaata aagataatta
     7141 tcgccgtgat gaaaacgata tctatgacat tgccgattat taccagaacg attttggccg
     7201 taaaaatagc ttttctgcca atatgagcca gtcattgcca gaaggctggg gttctgtgtc
     7261 cttaagtacg ttatggcgag attactgggg gcgtagcggc agcagtaagg attatcagtt
     7321 gagttattcc aataactggc ggcggataag ctataccctc gcggcaagcc aggcgtatgg
     7381 cgagaatcat catgaagaga aacgttttaa tatttttata tcaattccct gtgattgggg
     7441 tgatgacgtt acgacgcctc gtcggcaaat atatatgtct aactcaacga cgtttgatga
     7501 tcagggtttt gcctcaaata atacgggatt atcaggaaca gtagggagtc gggatcagtt
     7561 caattatggt gtcaacctga gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa
     7621 tttgacctgg aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt cgagtactta
     7681 tcgacagact ggagccagtg tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct
     7741 ggcgaaccgt ctttccgaaa cgtttgctgt gatgaatgcg ccaggaatta aagatgctta
     7801 tgtcaatggg caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat acgacggaat
     7861 gacaccttat cgggaaaatc acctgatgct ggatgtgtcg caaagcgata gcgaagcaga
     7921 attacgtggc aaccggaaaa ttgccgcccc ttatcgcggc gcggttgtac tggttaattt
     7981 tgataccgat cagcgcaagc cctggtttat aaaagcgtta agagcggatg gtcaaccatt
     8041 aacgtttggt tatgaagtca atgatatcca tggtcataat attggcgttg tcggccaggg
     8101 aagccagtta tttattcgca ccaatgaaat accgccattg gttaatgtag caattgataa
     8161 gcaacaagga ctttcatgca caatcacctt cggtaaagag attgatgaaa gtagaaatta
     8221 tatttgccag taatagtcag gtggttctat ggaaattcgc ataatgctat ttatattaat
     8281 gatgatggtt atgcctgtga gctatgcggc atgttatagt gagttatctg ttcagcacaa
     8341 tttggttgtt caggggaatt ttgcacttac tcaaacacaa atagcgacat atgagcataa
     8401 ttttaatgat tcgtcatgcg tgagtacaaa tactatcacc cctatgagcc catcggatat
     8461 tattgttgga ctttataacg ataccattaa attaaattta cattttgaat ggaccaataa
     8521 aaacaacatc acgttgtcaa ataatcagag cagtttcacc agtggttatt cggtaacggt
     8581 gacacctgcg gccagtaatg caaaagtgaa tatttctgcg gggggcggtg gttcagtgat
     8641 gattaatggt gttgcgacat tatccagtgc ttcatcatcg acacgcggga gtgccgcagt
     8701 acaatttcta ctgtgtttat taggtggcaa gtcatgggat gcatgtgtaa atagctacag
     8761 aaatgcattg gcacaaaatg caggtgtcta ttccttcaat ctgacattgt catacaaccc
     8821 gataaccaca acctgcaaac aggacgattt attaattact ttagacagta ttcccgtttc
     8881 acaattacca gccacaggta acaaagcaac aataaatagt aaaaaaaggg atattattct
     8941 gcgttgtaaa aatttattag gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt
     9001 atcaagttct gacttgttaa ccaacagcga cacaatactg aaaggtgcgg aagataatgc
     9061 gtaggattta ttcttgaaag taatggttcg ccagtcacac ttttaaatat cactaacagc
     9121 agtaaaggat acacaaattt aaaagaaatt gcggcaaagt caaaacttac agatacaacg
     9181 gtttcaattc cgataacagc cagttactac gtctacgata caaacaaagt taaatctggc
     9241 gcactggagg caaccgcatt aatcaacgtg aaatacgact aataacaagc aagacgagca
     9301 agtggctaat aataaaaaat aacaaggtgt ctgcatttcc ccctcatgat gagggggctt
     9361 tttttagcga tgataaaaaa tctcaccgtc gtaggcttta atgattttac cgtcggtgtc
     9421 gctgatcaac acgtacgcgc cacccatata ggtccagtgc gtcccggcat caggcgcggg
     9481 caggttacgt aactgccact gcttaatgtt gtactcatcg gtgcggtaca tctctgggac
     9541 ggtatcacct ggtttgaaat gcttaaagtc agcaatgaaa ctacttaact catacttaga
     9601 aattcccgag gtattggtcg aacccgcggt ggctggtgcg gcccaggcgg cactggtaac
     9661 cagcaaaagc acacccagca acatcttatt tttaatggtc ataaactctc cactttgggg
     9721 atctgtaata tcagtcgcat atctttcctg ccatacaata taactgacaa gcgatatttt
     9781 gttagtgatc ccatgatttc gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt
     9841 agcgagcaga gagtcagcgg aaatagctga tttaagaatc atcctgggct gaagataaca
     9901 tgcaagccta aaggattgag ttatcgcatt atgcccatga agccatgtac gcccatatac
     9961 acaccgacta agccaatcaa cagactggaa aaataggggg cgcgtttagc gagagtgtta
    10021 aatccgctcc agcgttttgc gacctgctga acgctgattg ctgcgccaac gcctacggtg
    10081 acaagcgtta acgccaggcc aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg
    10141 gctttcaact gaatgcatat caacagcacg gtaattgctg ccgggcaggg gataaggcca
    10201 ccggttaagc caaataacaa aatttgccag ttgatgacct ctctaccatc aaagcgtcgt
    10261 ttaatgtcat tggcatgggc tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg
    10321 tgatgatgtc cttggtcgtg gtgatgttcg tggtcttgat gatgttcgtg atcgtgatga
    10381 tggtgatgat gctcataatc atgcccgtgc atattctcca gccagttgcg ttcgccgcgc
    10441 caggtacgcc agaacatcca gaacacggtg ccaataatga tcactgcgga aatcagctgg
    10501 agccacggtt ctgctgattg agcagtaaag cgcttactga tcaccatccc gccaaaggca
    10561 atcaaccaga ccaccgcggt atgcgaaatg gttgctgcca gtccgagcat caccgcctgt
    10621 ttaatggtgc ctttgatggc gatgataaac gccgccatca tcgtttttga gtgccctggt
    10681 tccaggccat gaagcgcacc aagtaagatg gcgctgggga tgaagaacca ggcgtttcct
    10741 tgctgaagaa gagttgtaaa ttcggtcatg ataataattc ttagtattaa ttcggcaatc
    10801 tgattctact cccccccagt acctgatact accccccagt agattaatag tgctatgatt
    10861 tttcatattc ttgttaacca ggtgttgcca tgtctcatac aatccgtgat aaacagaaac
    10921 tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc gctcaagaaa atgctcgacg
    10981 agccgcacga atgcgctgca gttttacaac agattgctgc tatccgtgga gcggtaaacg
    11041 gtctgatgcg ggaagtgatt aaaggtcatc tgacggaaca catcgttcac cagggggatg
    11101 agctaaaacg tgaagaagat ctggatgtcg ttctgaaggt gctggattca tatatcaaat
    11161 aatttattaa cgcgattgta aaactgccgt ttttcctcgt ttacaacggg tgcgctggac
    11221 attaccatcc tcctctgcga tttatcatcg caaccaaacg actcggggtg cccttctgcg
    11281 tgaaggctga gaaatacccg tatcacctga tctggataat gccagcgtag ggaagtcacg
    11341 gaccaccagg tcattgcttc ttcacgttat ggcaggagca aactatgcaa gtcgacctgc
    11401 tgagttcagc gcaatctgcg cacgcgttac acctttttca ccaacattcc cctcttgtgc
    11461 actgcatgac caatgatgtg gtgcaaacct ttaccgccaa taccttgctg gcgctcggtg
    11521 catcgccagc gatggttatc gaaaccgaag aggccagtca gtttgcggct atcgccagtg
    11581 ccttgctgat taacgttggc acactgacgc agccacgcgc acaggcgatg cgtgctgccg
    11641 ttgagcaagc aaaaagctct caaacacgct ggacgcttga tccagtagcg gttggtgcgc
    11701 tcgattatcg ccgccatttt tgtcatgaac ttttatcttt taaaccggca gcgatacgtg
    11761 gtaatgcttc ggaaatcatg gcattagctg gcattgctaa tggcggacgg ggagtggata
    11821 ccactgacgc cgcagctaac gcgatacccg ctgcacaaac actggcacgg gaaactggcg
    11881 caatcgtcgt ggtcactggc gaggtggatt atgttaccga tggacatcgt atcattggta
    11941 ttcacggtgg cgatccgtta atgaccaaag tggtaggaac tggctgtgca ttatcggcgg
    12001 ttgtcgctgc ctgctgtgcg ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc
    12061 actggatgaa acaagccgga gaacgcgcag tcgccagaag cgaggggcca ggcagttttg
    12121 ttccacattt ccttgatgcg ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat
    12181 taacgctctg acgattgccg gtactgatcc gagtggtggt gcggggattc tggccgatct
    12241 taaaaccttc tcggcacttg gcgcttatgg ttgctcagtt attactgcac tggtggcgca
    12301 aaatacccgt ggcgtacagt cggtgtatcg cattgagcct gattttgtcg ccgcccagct
    12361 cgattcggtg ttcagcgatg tgcgaatcga taccactaaa atcggtatgt tggcggaaac
    12421 cgatattgtt gaagcggtgg cagaacggtt gcaacgttat cagatccaaa acgtggtact
    12481 cgacaccgtt atgctggcaa aaagcggcga cccgctgctt tcaccttcgg cggttgctac
    12541 gctgcgcagt cgattattgc cacaggtttc attaataacg ccaaacttgc ccgaagctgc
    12601 cgccttgctc gacgcgccac acgcgcgcac cgaacaggaa atgctggaac aagggcgatc
    12661 gctgttggcg atgggctgtg gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca
    12721 aagcccggac tggctgttta cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat
    12781 gaccaaaaac acccacggca ctggttgtac actctctgcg gcgttggctg cactacgccc
    12841 gcgccataca aactgggctg acaccgtaca ggaggcaaaa agctggcttt catcggcgtt
    12901 agcccaggcc gacacgctgg aagttggtca cggtattggt ccggttcacc acttccacgc
    12961 ctggtggtga gcaccgtact atagctgctt tggttattgt cagcctttgg cactgacatt
    13021 cagggatgaa accaatgcag ctaagaatta ccagtcgaaa aaaactcacc gctctattat
    13081 gcgctcttgg gctaatttcc atcgttgcaa tttatccgcg tcagacggtg aattttttct
    13141 attcgacagc agttcagatt acagactaca tccacttcta cggttatcgc ccggttaaat
    13201 ctttcgctat tcgcattcct gccagttaca ccattcacgg attagatgtt tcgcgctggc
    13261 aggagcgtat cgactggcag cgggtggcaa aaatgcgcga caacgatatc cgcttacagt
    13321 ttgcttttat taaggctacg gaaggcgaaa agctggtgga cccctatttt tcgcgtaact
    13381 ggcaattaag ccgcgaaaat ggcctgctgc gcggggcgta tcattatttc tccccgtcgg
    13441 tatccgcttc agttcaggcg cgattatttc tgcaaacggt ggatttctca caaggcgatt
    13501 tccctgccgt gctggacgta gaagaacggg gaaaattatc ggcaaaagaa ttacgcaagc
    13561 gggtaagtca gtggctaaaa atggtcgaaa aacgcacggg aaaaaagccg attatttact
    13621 caggagccgt tttttatcac accaatctgg cgggctattt caatgaatat ccgtggtggg
    13681 tggctcacta ttatcaacgt cgtccggaca atgacggcat ggcctggcgc ttctggcagc
    13741 attccgaccg tggacaggta gatggcatca atggtccggt ggattttaat gtgtttaatg
    13801 gcacggtgga tgagttgcag gcattcgttg atgggattaa agaaacgcct taaataagaa
    13861 gcagtgatat actcccacca gactccctgg caggaaaaag acaaatggaa caagcgcata
    13921 cccagctgat tgcccaactg aacgagcgta ttttagcggc ggataacacg ccgctttata
    13981 ttaagtttgc cgaaacggta aaaaatgccg tgcgcagcgg ggtgctggag catggcaata
    14041 ttttgcccgg tgagcgtgat ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca
    14101 aggcgatgca ggcgctggaa gaagagggcg tggtgacgcg ttcgcgcggt tacggcacgc
    14161 agatcaacaa catcttcgaa tactcgttga aagaagcgag aggtttttct cagcaggtgg
    14221 tattgcgcgg gaaaaagccc gatacgctat gggttaataa gcgtgtcgtg aaatgtcccg
    14281 aagaagttgc gcagcagctg gcggtcgaag cgggaagtga tgtctttttg cttaagcgta
    14341 ttcgctatgt cgatgaagaa gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa
    14401 ttcatgatgt tgatgccatc ggaatttctc tttatgacta tttccgcagc cagcacattt
    14461 acccacaacg tacgcgttcc cgcgttagcg cccggatgcc ggatgccgag tttcagtcac
    14521 atattcagtt agatagcaaa ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac
    14581 agcaacggcc tattgagtac agcattagcc actgtcgcag cgatctatac gtttttgtgt
    14641 gcgaggagta gttcctcgcg cgttggcgca caatcaccgc cccgatgccc gacaacccac
    14701 gacgccactg cattgcccag taatacggca tccgccagtg gcagaccaga ggccagcccg
    14761 gcaagtacgc cacctgcatg actgtcgccc gccccaatgg tgtctacaac ttgcgttgga
    14821 aatgcccgaa tgcagccaga agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga
    14881 cgaacgatca acggcgcggc aaatttctcc tgccattgct tgccaagtgt tgttatctct
    14941 gcagataaag caaaacgttc ggcggcaatc tcagcctctt gacgattgag tgacactaaa
    15001 ggtcgacagg ccatgatccg cgccagtaat gcgtccggga tatcgccaat acgtgggcca
    15061 aaatcgataa acggcgtcac gtcttgcagc ttttccagcc attccactaa caattcgccg
    15121 cagggcgagg ccagttgata accggaaaaa tagagcaggt tgccaggcgc aacggttaat
    15181 cgtgccaacc attggcgatt ccattgattt tccacaccgc tgaatgacat aaaagtgcgt
    15241 tcgccatccg gctcaaccag tgccagacac cagccgttat cgctttcggc gttatcgatc
    15301 aggctgatta acccctcttt cgccatccgg ttgcgaataa tctccgccca cacgccctga
    15361 ccgagcggca aggcattacc cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc
    15421 agtgcgcagc cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca cccacgccag
    15481 gggagggcat aagcgtcggc gatcacgtca atgaccgccg cgccgacgac catcaccggc
    15541 tgacgcgtgg ttaattcagg cagcagtgtg tgtaatcgag cgccgctcat accgcctccc
    15601 gttgttgacg atatttcgcc agcgctgtgg catagcggtt gaaatcaagc tgatttaccg
    15661 catccagctc cgcctttaac gcaggatcga cagcgttaat gccatgcaac gcgccgcaaa
    15721 tcgccgtcgc catagcacct atggtgtctg tgtcgccgcc aagattggcg cacaggacgg
    15781 cgcagcggtt tgggtcggtt tgtgccagtt caactagcgc aatggcgcac ggaacggact
    15841 caatagtgct ggtgcctgcg ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc
    15901 cgtcggcatt acgcacaatt ttcagcgcaa tctccagacg tgccgccagt gaggcgctga
    15961 acgtggtggt gcgtttttgc tgtgcatgtc gcgcaattga aggcaatgaa tcaacaatcg
    16021 ctgaccagcg ttctccgtca atggcacgag aaatcgccca tgcgatgact accgcgcctg
    16081 caaccgccag atcggattta tgggtcgggc tggacgccag cgccacatca tcaataaagg
    16141 aatcaacatc acgcgctgga agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt
    16201 tggttacgcc gttgttttcc agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg
    16261 caatcttcga ggtcggacct agtacgtttt tgttaaaggc gtcgaaacgc aacgcccagt
    16321 cgagaatatt acgcccaatc agattcggat cgatcttgcc ttcacattcc agtaacgcat
    16381 ccgccagaca caggccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc
    16441 cgcgttattc tcctttggtc caggcagaaa acggtcaatc cagccaaagt gtgctttaac
    16501 gcggctgcgt ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata
    16561 aagagcaccg agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatct
    16621 cgatcatcga ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac
    16681 agcacggcga taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa
    16741 gtcagtccgt ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga
    16801 cgatagccga gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg
    16861 gtacgcatat gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa
    16921 tcgtaactta cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaattgaca
    16981 ggtaatgtaa ggtacgcgat gacaatacca cataataata ccttttgaat acctttgcgg
    17041 tgaaaacttg accgcgctaa cattttttct gatgaatcga gccaacagaa aacgctgaaa
    17101 aaacatccaa aagatggaaa aactcgcccg taacgaccag gattatgatt gcccgcaaaa
    17161 ggcgggccag gacaatccat agccgatatc caatcggaat ttacgggagc atagtaatga
    17221 cagatattgc gcagttgctt ggcaaagacg ccgacaacct tttacagcac cgttgtatga
    17281 ctattccttc tgaccagctt tatctccccg gacatgacta cgtagaccgc gtgatgattg
    17341 acaataatcg cccgccagcg gtgttacgta atatgcagac gttgtacaac accgggcgtc
    17401 tggctggcac aggatatctt tctattctgc cggttgacca gggcgttgag cactctgccg
    17461 gagcttcatt tgctgctaac ccgctctact ttgacccgaa aaacattgtt gaactggcga
    17521 tcgaagcggg ctgtaactgt gtggcatcaa cttacggcgt gttggtgtcg gtatcgcggc
    17581 gctatgcgca tcgcattcca tttctcgtca aacttaatca caacgagacg ctaagttacc
    17641 cgaataccta cgatcaaacg ctgtatgcca gcgtggagca ggccttcaac atgggcgcgg
    17701 tggcggttgg tgcgactatc tattttggct cggaagagtc acgtcgccag attgaagaaa
    17761 tttctgcggc ctttgaacgc gcgcacgagc tgggcatggt ggcagtgctg tgggcctatt
    17821 tgcgaaactc cgcctttaag aaagatggcg ttgattacca tgtttccgcc gacctgactg
    17881 gtcaggcgaa ccatctggcg gcaaccatag gtgcagatat cgtcaaacaa aaaatggcgg
    17941 aaaataacgg cggctataaa gcgattaatt acggttatac cgacgatcgc gtgtacagca
    18001 agttaaccag cgaaaacccg attgatctgg tgcgttatca gttagctaac tgctatatgg
    18061 gccgggctgg gttgataaac tccggcggtg ctgcaggcgg tgaaactgac ctcagcgatg
    18121 cagtgcgtac tgcggttatc aacaaacgcg caggcggaat ggggctgatt cttggacgta
    18181 aggcgttcaa gaaatcgatg gctgacggcg tgaaactgat taatgccgtg caggacgttt
    18241 atctcgatag caaaattact attgcctgat acgcctcata cgaacagcac caccccgtag
    18301 gacggataag gcgtttacac tgcatccggc aacctatgcc tgatgcgacg cggaagcgtc
    18361 ttatcatgcc tacatagcac tgccacgtat gtttacaccg catccggcat aaaaacacgc
    18421 gcactttgct acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacaa
    18481 ttttcatatt gtcgtttttg tgatcgttat ctcgacattt aaaagcaaat aatttcatta
    18541 tattttgaaa tcgaaagcaa acgacaagat atgaaaatgt acgtggtatc gacaaagcag
    18601 atgctgaaca acgcacagcg cggcggttat gcggttccgg cattcaatat tcacaatctc
    18661 gaaacgatgc aagtggtggt ggaaaccgct gccaacctgc atgcgccggt catcatcgcc
    18721 ggaacgcctg gcacatttac tcatgctggt acagaaaatc tgttggcgct ggtcagcgcg
    18781 atggcgaagc aatatcacca tccactggca attcatctcg accatcacac gaaatttgac
    18841 gatatcgctc agaaggttcg ttctggcgtg cgctcagtca tgattgacgc ctcgcatttg
    18901 ccttttgcgc aaaatatttc acggctcaaa gaggtggtgg atttttgcca tcgctttgat
    18961 gtcagcgtcg aggcggagct ggggcaactt ggcggccagg aagatgatgt gcaagtcaat
    19021 gaagccgatg cgttgtacac caacctcgca caggcgcgtg aatttgccga ggcaaccggg
    19081 attgattccc tggcggtcgc catcggcacg gctcacggta tgtatgccag cgcaccggtg
    19141 cttgattttt ctcgactgga gaacattcgc cagtgggtga acttaccgct ggtgctgcat
    19201 ggcgcgtcag gattatcgac taaggatatt cagcaaacca tcaaactggg gatatgcaaa
    19261 atcaacgttg caacggagct gaaaaatgcc ttctcgcagt cgttaaaaaa ttacctgacc
    19321 gagcaccccg aagcgaccga tccccgggat tatttgcagt cggctaaatc cgcaatgcgc
    19381 gatgtggtga gcaaagtgat tgccgattgt ggctgcgagg gcagggcata acgcacctgc
    19441 catttaacaa ggaaaaaaca tgaaaacgtt aattgcccgg cataaagctg gtgaacatat
    19501 cggcatatgt tcagtctgtt ctgcccatcc gttggttatc gaagcggcgc tggcatttga
    19561 tcgcaacagc acgcgcaaag tgctgattga agcaacgtca aaccaggtca atcaatttgg
    19621 cggttatacc ggaatgacac cggcagactt tcgcgaattt gtttttacga ttgccgataa
    19681 agttgggttt gcacgcgaac gcattattct cggcggcgat catctggggc caaactgctg
    19741 gcagcaagaa aatgcggatg cggcgatgga aaaatccgtc gagctggtaa aagcatatgt
    19801 tcgtgctggc ttcagtaaaa ttcatcttga tgcgtcaatg tcctgcgcgg gggatcccat
    19861 accgttagca ccagaaacgg ttgcggaacg agctgttgtg ctttgctttg ctgcggaaag
    19921 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    19981 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    20041 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    20101 acgtgtgatt gccatcgtgg tgcagccggg tgtggaattt gatcacagca atattatcca
    20161 ttatcagccg caggaagcgc aggcgctggc gcaatggata gaaaacaccc gaatggttta
    20221 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    20281 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    20341 actggcacaa attgagcagg aacttatcgc ccccgaaaat cgcagcggtt gcctggcggt
    20401 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gcacgggttt
    20461 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    20521 gcatagtcgg attaaaaata gcgttgaaac gatgatggtg aatctgcaag gcgtggacat
    20581 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    20641 attatcagca atgccgcatc agcttattat gaataaaatt tatgatgttt tgcgcgccta
    20701 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    20761 ttcttttgtc gatcgtagcg aagttttaac ctatatcggt aatgagatgc tcgcgaaagg
    20821 tgtggttcat gatacctggc cacaggcatt aattgccaga gaagcagaat tccctaccgg
    20881 gataatgctt gagcagcacg ccattgcaat accgcattgt gaggcgattc atgctaagtc
    20941 gtcagccatt tatctgttaa ggccaacaaa taaagttcat tttcagcaag cggatgatga
    21001 taacgacgtg gcggtatcgt tggttattgc gttgattgtg gaaaatccgc agcagcaatt
    21061 gaaactttta cgctgtttat ttggcaagtt acaacagccc gatatcgtcg agacactaat
    21121 tactcttcct gaaactcagt taaaggaata cttcacaaag tatgttttag attcagacga
    21181 ataaatccct ctgtaacaat aaaaaggact atttatgaaa cgcaagatta ttgtcgcttg
    21241 cggaggcgcg gttgcgacct ctacgatggc ggcggaagaa attaaagagt tgtgtcagag
    21301 tcataatatt cctgttgaat taatccagtg tcgggttaat gaaatagaaa cctatatgga
    21361 tggtgtgcat ttgatatgta ccactgccag ggtggatcgt agttttggcg atattccgtt
    21421 agttcacggc atgccttttg tttctggtgt cggtatcgaa gcattacaaa ataaaattct
    21481 gactatctta caggggtgac ctatgttttc agaagtcatg cgttatattc tcgacctcgg
    21541 ccctacagtg atgctgccgg ttgtcatcat tattttttct aaaatattag gcatgaaggc
    21601 aggcgattgc tttaaagcgg gtctgcatat cgggattggc tttgttggca ttggccttgt
    21661 gattggctta atgctggatt ccattggtcc ggcggcgaaa gcgatggcgg aaaatttcga
    21721 cctgaatctg catgtggtcg atgtcggctg gccgggctct tcaccaatga cctgggcgtc
    21781 gcaaattgcg ctggtggcga ttccgattgc gatattggtt aacgtggcga tgttactgac
    21841 ccgtatgacg cgggtggtaa atgttgatat ctggaatatc tggcatatga ccttcaccgg
    21901 cagcgttgct gcacctggca accggttcat ggatgatagg gatggcgggt gtggtaattc
    21961 acgcggcgtt tgtttataag ctcggcgact ggtttgcccg cgatacccga aatttctttg
    22021 agctggaagg tattgctatt ccgcacggta cgtcggcgta tatggggccg attgcggtgc
    22081 tggtcgatgc tatcatcgag aaaatcccag gcgttaaccg aattaaattt agcgccgacg
    22141 atattcagcg caaatttggc ccttttggcg aacctgtcac cgtgggtttt gtgatggagc
    22201 tgattatcgg catcctcgcg ggttacgatg tcaaaggtgt attgcagctg gcggtaaaaa
    22261 cggcggcagt tatgctgctg atgccacgga tgattaaacc catcatggat ggtttaacgc
    22321 ccatcgctaa gcaggcccgt agtcgtttac aggcgaagtt cggcggtcag gagttcctga
    22381 ttggcctgga tcccgcatta ctgctggggc atacggcggt ggtatcggca agcctgattt
    22441 ttattccgct caccatttta attgctgttt gtgttccggg taatcaggta ctgccgtttg
    22501 gcgatcttgc cactatcggc ttcttcgtgg cgatggcggt cgcggtgcat cgtggaaatc
    22561 tgttccgcac cttaatctcg ggtgtcatca ttatgagcat caccctgtgg atcgcgacgc
    22621 aaactattgg tttgcacacc caactggcgg ctaatgctgg ggcgttaaaa gccgggggta
    22681 tggtggcttc aatggatcag ggcggttctc ccattacctg gttactgatt caggttttct
    22741 ccccgcaaaa tattcccggt ttcattatta tcggcgcaat ttatctgacc ggtattttca
    22801 tgacctggcg tagagcgcgt ggttttacta aacaagagaa agccgttctc gcagaataat
    22861 ttttacctga gggggaggtt atccccctta acaatcagga gtttttatga aatcagtagt
    22921 gaatgatact gatggtatcg tgcgcgttgc aaaaagcgtc attcctgaaa ttaaacatca
    22981 ggatgaggtg cgggtaaaaa ttgccagctc gggcttatgt ggttccgatt tacccaggat
    23041 atttaaaaat ggtgcacatt attatccaat aacgttaggc catgaattta gcggctatat
    23101 tgatgcggtg ggatccggtg ttgatgattt acaccctggc gatgcggttg cctgtgtgcc
    23161 gttattaccc tgttttactt gtccagagtg tttgaaaggg ttttattccc agtgcgcaaa
    23221 atatgatttt attggctcgc ggcgtgatgg tggatttgct gaatatattg tcgttaagcg
    23281 aaaaaatgtc tttgctctac ccacggatat gcctattgag gatggggctt ttattgagcc
    23341 gattaccgtt ggcctgcatg cttttcattt agcgcaaggt tgtgagaata aaaacgttat
    23401 tattattggt gccggaacca ttggcctgct ggctattcag tgcgctgtcg cgctgggagc
    23461 aaagagtgtg acggcgatcg acattagctc agaaaaactg gcactggcaa aatctttcgg
    23521 tgcgatgcaa acatttaaca gtagcgaaat gagcgcgccg caaatgcaga gcgttttacg
    23581 cgaactgcgc tttaatcagc ttatcctcga gacggctggc gtaccgcaaa ctgtcgaact
    23641 ggcggtagag attgccggtc ctcatgccca actggcgctg gtgggcacgt tgcatcagga
    23701 tctgcattta acatcggcaa cgtttggcaa aatattgcgt aaagagctga cggttatcgg
    23761 cagttggatg aactattcca gcccttggcc ggggcaggag tgggaaacgg cgagccggtt
    23821 gctgacagaa cgtaagttaa gcctggatcc attaatcgct caccgtggaa gctttgaaag
    23881 cttcacccag gcggtgcgtg acatcgctcg taatgccatg ccgggcaaag tgttgctcat
    23941 tccatgaaac cgcgggccag cgtgatgctg gcccggtatt gtgcaaaaca gatcattcac
    24001 caatggtccc ccttcgttta cactagccac aattgaatat gggtaaatga cgatgaattc
    24061 attcgagcga aggaataaga tcatccaatt agtgaatgaa cagggaaccg tgcttgttca
    24121 ggatctggcg ggagtatttg ctgcctcgga agcgacaatc cgtgccgatt tgcgctttct
    24181 cgaacaaaaa ggcgtggtta cgcgctttca tggcggtgcg gcgaaaataa tgtccggtaa
    24241 tagtgaaacc gagacccagg aagtcgggtt taaagagcga tttcagctcg ccagcgcgcc
    24301 aaaaaacaga atagcgcagg aggcagtcaa aatgatccat gaagggatga ccgttattct
    24361 cgacagcgga agtaccacaa tgcttatcgc cgaaggatta atgaccgcta aaaatatcac
    24421 ggtgattacc aacagtctcc cggcagcgtt tgccctttcc gaaaataaag acattactct
    24481 ggtcgtctgt ggtggcacgg tccgccataa aacgcgctcg atgcatggtt ccattgccga
    24541 gcgttcattg caggatatta atgccgattt aatgtttgtc ggtgctgatg gtattgatgc
    24601 ggttaatggt attacgacct ttaatgaagg ttattcgatt agcggggcga tggtcaccgc
    24661 cgcaaataaa gtgattgccg ttctcgattc atcgaaattt aatcgtcgcg gttttaatca
    24721 ggtactgcca atcgaaaaaa ttgacattat tgttaccgat gatgctgtgt cagaggtgga
    24781 taaattggca ttgcagaaga cacgggtaaa attaattacg gtgtagtatt gttctgtatg
    24841 ccggataagg tgtttacacc gcatccggca ttacggattc tccctggttc aagagagaat
    24901 agtttgatta acgcaataat ggacaatctg gtggtaatcg acaacgcaac gccgccggaa
    24961 gtatttcaat atggaagttt tgcccactca acggttcgcc atcaagatta aaagtgattt
    25021 cgtgtggggc ttgtatatca aaccacgacg aagcgccttc gataatattc gggttatctt
    25081 cgtcagattt taaggttgat acgagagccg gaagaatttc atcgccggta aaaatgcgca
    25141 gttgcagcaa gccatcgtta attaacgcgt tcgggcacaa ttgttgaccg ccaccggcct
    25201 gacgcccgtt accaatacca atgaccaggg cgtcaccttg ccagtgaaag ttttcaccgc
    25261 ggatttcaca acggtccggt tgcagagtat ccatgcgcat taagccatga atgatgtaag
    25321 agacgccacc cagcgcggct tttaattttt ccggcgtttc tgtggtaata cgcgtcccaa
    25381 atccgcctgt cgccatatta ataaaacagg tttgtttgtt gacctgcgcc atatctatcg
    25441 caatggcatt accggcaatt gccagtttca gcgccttatc cagtgcctca ggaatcccta
    25501 cactggtggc aaaatcattg gcggttccta atggcaaaat tcccagcgcg ggtatgtcat
    25561 ccccctcaca ctgaatcaac gccgtagaaa cttcattaat ggtgccatcg ccaccaccgg
    25621 caatcaccgt tgcgacgccc aacttccggg cctcctctac aaatcgtgcg gcatcacctt
    25681 tctcccaggt gacccgcaca tggatcgtca ttccttcctc acgcaacagc ataattgctt
    25741 cgcgcaaggg tagattgtca gtacttttgc cattaagaat cagtaagctg gcgggaaatt
    25801 ctgccatgat cgtatgtgcc tttatgattg gtctgtagat agtgtagagc agaaaacaaa
    25861 aggtggagtc agaacaggat gaaagtcgga ggataaaaag tcagttcaag tatggaggat
    25921 tcagtggtta ccaccaggag cttgcaatgg ggacagcgaa tactaacggc tgccaccgat
    25981 aaatttcaaa aaagagcata tacctaatat tcaactaaac agtggcatct tcaatataat
    26041 atattaaagc ccccatggag ttaccctgaa gggcctcaat gtccgtaatt cctacttatg
    26101 taggaaatgt tgtacagaac atttattata atcctattca attataataa tcatgccatt
    26161 attatattta aacactagag agtgtcgttg gtatttaatg ggggaaggtg agatgaaaaa
    26221 gatagctgct atatcattaa ttagtatttt tcttatatct ggttgtgctg tgcataatga
    26281 tgaaacaagt atcggtaaat ttggtcttgc atataaaagt aatattcagc gtaaactcga
    26341 taaccaatac tacaccgaag ccgaagcttc tttagccagg ggcagaatat ctggtgcaga
    26401 aaatatagta aaaaatgatg cagcccattt ctgtgttact cagggcaaaa aaatgcagat
    26461 agttgacctg aagacagaag gtgcaggatt acatggcgtc gctcgtctga cattcaaatg
    26521 tggagagtga gaatattttt tggtaagcgt caaacatgcg cgttctggct gtgcgtagcc
    26581 ggaacctggg cgagcacgat accgttacgt gaaaggcatt gtgctatgaa gggggattct
    26641 atcgatgtgg tcaatggaag acggttatca gggatcaatc cttctttaca gcttatgtac
    26701 aaaaaataag cccgtgtaag ggagattaca caggctaagg aggtggttcc tggtacagct
    26761 agcattttat ggattatgtt tttcagcgaa acggatgata accttaataa atgcagctgt
    26821 atgtgatcgg tttctaagaa ttttccatcc gggaaaaata atcgaaatta atcacttacc
    26881 gtgggggtta cgcgtggttt ccccggagaa attacgcatt agcagcgcgt aatttagctc
    26941 aagatcctgc gggaccggga gccacacagt ataaccatcg cctggtgcga ccggcatagc
    27001 ttcgcctttg gcgttttcca tgtgctcaag ggtaaagtta atgttgcctt gcggcgtcat
    27061 cagctcaaga ctgtcgccaa cggagaattt atttttcacc gctaccgctg cgaggtcccc
    27121 cttgcgctca ccggtaaact caccaacaaa ctgctggcgg tcagaaactg aataaccgta
    27181 ttcgtagttc tgataatcgt cgtgagtatg acgacgcagg aaaccttcgg tatagccacg
    27241 atgcgccaga ccttccagag tttccagcag gctggtatcg aacggttttc ccgcagcggc
    27301 gtcatcgata gctttgcggt aaacctgtgc ggtgcgtgcg caatagtaga aagatttggt
    27361 acgaccttcg attttcagcg aatgcacgcc cattttggtc aggcgttcta catgggcgat
    27421 ggcgcgcaga tctttcgagt tcatgatgta agtgccgtgc tcatcttcaa acgcggtcat
    27481 atactcgccc ggacgctggg cttcttcgat cataaacact ttgtcggttg gcgcgccgat
    27541 acccagcgtc ggctcaacat tttgcaccgg aatcggctcg tacttgtgta cgatgttgcc
    27601 aacgtcatct tctttccctt cctggacgtt gtactcccag cggcaggcgt tggtgcaggt
    27661 gccctggttc gggtcgcgct tgttgatata gccagagagc aggcagcgac cggagtaggc
    27721 catgcacagt gcgccgtgaa cgaagatctc gatctccata tccggcacct gattatggat
    27781 ctcttcaatc tcttccagcg acagctcgcg agagaggatc acgcgggtca gtcccatttg
    27841 ctgccagaat ttcaccgtcg cccagttcac ggcgttagcc tgcaccgaga ggtggatcgg
    27901 catttctggg aagtgctcac gcaccagcat aatcagccct ggatcggaca taatcagcgc
    27961 atccggcccc atttccacca ccggtttcag gtcacggata aaggttttca gcttggcgtt
    28021 gtgtggtgca atgttgacca cgacataaaa ctttttcccc agcgcgtggg cttcattgat
    28081 gccgagctga agattttcgt ggttgaattc gttgttgcgc acacgtaggg agtaacgcgg
    28141 ctggcccgca taaacagcat ctgcgccata agcgaaagcg taacgcatat ttttcagcgt
    28201 tcccgccggg gaaaggagtt ccggtttaaa cataattctc tcgttctgat gacaggtcag
    28261 atccgcttca cctgatgaag cggttagggg aggcccccac tttaagggcg ggcattgtag
    28321 cgctgtaggg gggatctggt aaagcgccgg acgccaacaa cccacgcccg gcatcgtaat
    28381 tacgtattgt ctttcaccgt ctggcttgtg ccattggctt taacggaggc aattccggtt
    28441 tcacgagatt gcgcggtggc gtacatctgg ctggagccga taatttgatg attagcggct
    28501 ttcagattga aatagaattt gccgtttttt tctcatagcg ctcttcttgc gggctgttgc
    28561 tacgcaccga cgcgatgccc ttttccgcag aggcttttga ggtatacagc tcgctggtga
    28621 ggatagtctc accattgccc gcttttagca caaaccggaa ctgattatca ctgctcttac
    28681 tgagttcaaa ccaaccagcc ataatgactc cttgattgaa attgcttatc gaaaacagca
    28741 tctaaaggtg ctgtttactg attaaagtat gagcgatttt ctgatgcctg ccagggcgga
    28801 gagataatgt cagtttgctg accatgcgtg gaataatgtt gccggatcgg gtctgaacgc
    28861 catctccggc taacaaagta atgtcgctaa aactaaacga tgcggcaggc gtcggcttcc
    28921 cagcggtaac caacgccgta aacggcgcgg ataaatgact gttcggcgtc gagagattcc
    28981 agcttgcggc gcagattttt aatgtggctg tcaatggtgc ggtcggttac tacgcggtag
    29041 tcgtcataaa gatgattgag caattgctcg cgggagaaca cttttcctga ttcgtgagag
    29101 agcgttttaa gcagacgaaa ttccgcaggc gtcaggtcaa gcattttacc gcgccatgaa
    29161 gcctgaaaac gaccttcgtc gataatcaac gggctttcgg catcctgttg ctgaagttcg
    29221 cgttgcggtt tacaacggcg caaaatggtt ttgacacgcg ctaccacttc ccgtgggctg
    29281 tacggcttgc agatgtagtc atcggcgcca atctccagcc ccagcaggcg gtcgatctct
    29341 tcgatttttg ccgtcaccat cacgatcgga acatcggaaa aacgacgaat ttcccggcat
    29401 aatgtcaggc catcggtgcc agggagcatc agatccaaca ggatcagatc cggtggtgtc
    29461 tggcgcacat acgccagtac ctgatcgccg tggctgataa gcgtcggcgc atagctcgca
    29521 gcacgcagat aatcaatgag caactgcccc agcttcggtt catcttccac gatcaaaata
    29581 cgcggtgtgt tttcgtcgat tggtaactcg gtcatacttc tctctgtaaa tcccgttcca
    29641 gcggtaactc tactgtaatg cttaccccgc caaaaggcga atgggcggca ataatgcggc
    29701 cattatgggc ttcaacaatg ttcaggcaaa tcgccagccc cagcccggaa ccgccgctgg
    29761 cgcggttgcg ggaaccttcg gtgcgataaa aacgttcaaa caatttttgt agctgatcgt
    29821 cactgacacc tggcgcactg tcggcaaagg tcaggcgcac cgttttgtcg tgctgctcgg
    29881 cagagatttt caggctgccg ccgctgtcag tgtagcgcag gctgttttcc agtaagttat
    29941 tgaataactg cattaaacgg tcgcgatcgc caaatacggt gatactgtct ggcagggaaa
    30001 attgcagttt caggccacgg ctggcgaatc gttcgcgaaa tgcgccgccc gccacttcca
    30061 gcagtgggat caaatctacc ggtgcttttt gataggcgag agcgccttca tcagacatcg
    30121 acaactgatg aagatcgtca accagtttgg tcagtgtacc gacctccgcc tgtaaagacg
    30181 ccaccgtctc cggcgtgaat ttgcgcacgc catcctgaat ggcttccagt tcaccgcgca
    30241 gcaccgctaa tggcgtacgc agttcgtgag aaatatcggc cataaaatcg cgccgcattt
    30301 gctggttttt ctccagcgtg ctggcgagct ggttgaagtc ttgcgccagt ttgcccagtt
    30361 catcttcact ggtgggcgtt acgcgggtag tgaaatcgcc cgccgccagt ttgtgcgtgc
    30421 catcgataag tcgttttacc ggtgccagca aaccgcgcgc cagcagaaag gtggcgagcg
    30481 ccgcgagtaa cgttgccagg gcgacgatca gccagctggt ttgccgctgt tgtttatcga
    30541 aattgatatc agtattgcgg gtcagccgct caacagggga ggcaatcacc gcgcccactt
    30601 ccgcaccgtt gaccagaatg ggtcgtcgtg taccgtcagg tggaatcggc gctcgcggac
    30661 caaccagtac tttgttgttt tgatcgacca cccagaactg ggtacgccag ccgtgcggcg
    30721 gcatacccgg gccgggttta tcttccgaat tatcgtgttc aaatgaacgc aggatctgaa
    30781 agacaaagcg atcgttgttg cgcaggaagc gccagttacc atgctgcgca tactgctcgc
    30841 caagcgcatc acttaacagc tgtaatcgct gttcattgcc gtgtttgatg taatcaataa
    30901 agccacgctc aaaactgata cgcaccgccc agtgcatact gatcagcaag acaatgcagg
    30961 tggcgaaaat cgccagaaac agtttgccgg taataccggg tcgccagaac ttcattgcgc
    31021 gctccttttt cgccgcgaaa tagctacatt ttgatgcgta tcgttcggca ctctggcaaa
    31081 gatgaacgcc ggaagggcga tgataaacgc catgctaagc caggtgtaca taaagacggt
    31141 ttgtgtggtg ccgctgtcga cgctgacatg ctgcgaacca aaaagcccca gcaacagccc
    31201 ggcgatggtg acgccgatac tcatcgacaa ttgcataatc atcgacagca ggctgtttcc
    31261 gctgctcgcc agattgtccg ggagatcttt cagcgtcagg gtgttcatgg aggagaaacg
    31321 ggtcgagttg accatccctt gtaaaaacag gacgaacggc aaaacgtagt accagcccaa
    31381 cagagcggta gtcataaaca acagggtgac cagcgacaga cccagcgtgg tcgctaccag
    31441 tacccgacga taaccaaagc gattcaccac ctgtaccaca attcgcttca ttcccatgct
    31501 gccaagcacc atcgggatca tcatcagtcc ggcatgaaac ggcgagaaac cgaggccaat
    31561 ttgcaggaaa accggtgtca taaagggcaa catgccactg ccaatacgtc cggcaaagct
    31621 ccccgccagg cccagcgaaa aggtacgagt gcggaacagt ttcagactga acagggcgcg
    31681 gttgttattt ctggcgtgca gcagataaag caccagtgcc accacgccaa ctgcgaccag
    31741 gccagcaatc gccagcggcg ataaacctgt acctttactg ccgtccagcg ccagggttaa
    31801 taccgccatg ccaaccgcca gcaataaaaa gccggaaaga tcaaagcgcc gcgtctgcat
    31861 ggtgtagttt ggcattaaca tcaatgtggc gatcgcaccg ataatcccca ccggaatgtt
    31921 gatcaaaaag atccagtgcc acgacgcgta ttctaccagc agaccgccga gcgccggacc
    31981 gagtagcgga ccgacctgac cgggtaacgt gacaaaggtc atcgccgcca tatattgctc
    32041 gcgcggtacg attttcatca ccgtcaacct gccgaccggc accatcatcg caccgccaac
    32101 gccctgtaac gcgcgtgcca gcaacagttc gttcagcgtg ccggaaagcg cgcaaaacag
    32161 tgaaccgaga gtaaacagca cgatggcggt aaagaaaata ttgcgcacgc cgactttgtc
    32221 cgccagccag ccgctggcgg gcagcatcac cgccacggtc agcacataag agacaatgac
    32281 catgtgcata tgcaacggac tttccccgag gctttgcgcc attgagggaa gggcggtgtt
    32341 tacgatggtg gtgtccagcg actgcataaa gaagccgaaa gccacaatcc ataattgcca
    32401 gcgggtgctg tcgggaagat ctgtcattta ctcggttacc gtttgtttag gtttacgcga
    32461 aaaacgcagc cgcagacggt cgaaaaagag atacaccacc ggcgtggtat acagcgtcag
    32521 gagctggctc atcaccagac cgccgacaat ggtgatcctc aggggttgcc gcagctccga
    32581 gccgtcaccg cccgacaata ccagcggcag cgcgccaaac agcgccgcca gggtggtcat
    32641 cataatcggg cgaaaacgca gcagacaggc ctggaaaata gcttcctgcg gcgtcaggtt
    32701 accgtgccgt tgggcttcaa gcgcaaaatc gaccatcata atggcgtttt tcttcacgat
    32761 gccgattaat agcatgatcc ctatcagggc gattaggctg aacggggcat tgaacagctc
    32821 cagcgccaac agcgctccaa cgcccgccga gggcagggtg gagagaatcg tcagcggatg
    32881 tacgtaactc tcgtaaagga tacccagcac gatatacacc gtggcgatgg cggcgataat
    32941 caggatcacc tgcgagttca tcgtctcctg gaaaacctgc gccgtgccgg caaaactgcc
    33001 gcgcaccgtc gaaggcacac caagctgggt cattgcgcga tcgatcgccg cactggcgtc
    33061 cgagagcgat tttccggtcg gcaggttgaa cgagatggtc gaggccgccg ataatccctg
    33121 atgattcacc gatagtgggg cattcgccgg ttgccattta gcgaaatacg acagcgggat
    33181 cgctttgcct tcgttattga taacgaacat tttttccagc gcactgatgt cctgggtata
    33241 gcgcggatcc acttccatca ccactttata ctggttcatc ggctggtaaa tggtcgagat
    33301 ttgccgctga ccgaaggcgt tatttaacag actgttggcg gcctgtacgt cgattcccag
    33361 ccgtgccatg gtgtcgcggt cgtaaaccag attcatctcc gcgccgttat cctgctgatc
    33421 ggagttcacg tccgccagtt ccggcaacgt cgccagtttt ttgcggattt tcggctccca
    33481 ttcgcgcagt gccgccaggt cgtcggataa caacgtgtac tggtagctgg cgttcgactg
    33541 acgcccacca acgcgaatat cttgtaccgc catcaggaac agattcgccc ccggttcttt
    33601 cgccagcttc acgcgcagac ggtcgataat ttgctgcgcc gtttcgctgc gttcgtcgcg
    33661 tggcttgagg gtgataaaca tcatcccact gttaactcgt gaaccgccgg taaagccggt
    33721 gacattatcc actgccggat cgtcacggat aattttcatg aaatcctgca atttgccgcg
    33781 catcgcctga aacgaaatac tctgatccgc ctgtatccca cccatcagta cgccggtgtc
    33841 ctgctccggg aagaaggttt tcgggatcga gatattcagc gcaatggtgc caagcagcac
    33901 cacgcccacc agacgggtat gattgagcac ccatttcagc gacttgccgt agccttgttg
    33961 cagggctacc aacatgcggc caaaaccacg cagtcgcttt tgctcacgcg gcttgctggc
    34021 ttttagcatc cagccgcaca tcattggcgt tagcgtcagg gaaaccagca acgaaatacc
    34081 aatggcgaca gaaagcgtca cggcaaactc gcgtaacagt cggcccggca atccgcccat
    34141 caacagcagc ggcaggaaca ccgccaccag tgacagactc atcgacagca ccgtaaagcc
    34201 gacttcgcga gtaccttgca gcgcggcctg tagtggtttc atccccgctt ccagatgacg
    34261 ggcaatgttt tccagcacca caatggcgtc atccaccacg aaaccagtag caatggtgag
    34321 cgccatcagc gaaagattat tgagactgaa tccgcacagg tacatcgccg caaacgtacc
    34381 aatcagcgaa accggcaccg caacggcggg aataatagtg gcgcgacccg agcgcaggaa
    34441 taaaaagacc accagaatca ccagcgccac cgagataatc agcgtttgct cgacttcttc
    34501 cagcgaggcg cgaatggtgg gggagcgatc ctgggcaatt tgcagatcaa tcgccgccgg
    34561 aatggtctcc tgcaactctg gtaattttgc ccggatgctg tctaccgtct ggataatatt
    34621 ggcttccggc agtttgcgga tcatcagtaa aatagccggt ttggcgttgg tcatcccggc
    34681 gttgcgcaca tcctgcactg agtcagtcac cgtcgccaca tcgcccagac gaaccgcgcc
    34741 gccgttgttg tagtgaataa tcagcggctg atattcagcg gcggttttca gttcatcgtt
    34801 ggtctggatc tgccagcggt gagtgccatc ttccagcgcc ccctgcggtt tacgcacgtt
    34861 ggcattgctg atggcagtgc gtacgtcgtc cagcgacacg ccctgattaa acagcgcctg
    34921 cgggttcagc ccgacgcgca cggcgggcag cgagctgcca ccgacatcga catcaccgac
    34981 accgtcgatt tgcgaaatcg tcggagccag ctgcgtcgag gcgaaatcgt acagttcacc
    35041 ctgcgaataa gtatcggatg tcagcgtgag gatcataatt ggcgcatccg acgggttcgc
    35101 tttgcgatag gtcgggcggc tgggcatccc gctgggcagc aaactttgtg cagcgttgat
    35161 cgccgcctgc acatcacgcg ctgcgccgtt gatatcccgg tcaaaatcaa actgcaaaat
    35221 aatgcgcgtg ctgccgagcg aactgctgga ggtcatttca ctaaccccgg caatgcgccc
    35281 aagttagcgc tccagcggcg tggcaacgga agacgccatt gtctccggcg acgcaccggg
    35341 cagcgaggcg ctgaccataa tcaccggaaa atcgacctgc ggcagcgggg cgaccggcag
    35401 catacggaaa cccagtatgc cgcacagggt aatggcgacc gacagtaaaa tcgtcgccac
    35461 cgggcggtaa atgaagaggg caaaaaactt cacttacgcc tcctcttcat ggcgagcaaa
    35521 gcgacttttg gtccacaata ccaagcggtc gaacagcaga taaatcaccg gcgtggtgaa
    35581 cagcgtcagc acctggctga caatcagacc gccgaccatg ccgataccta acggacgacg
    35641 cagttccgcg ccgaccccgg tactcaacat cagcggcagc gcgccaagca gagccgccag
    35701 ggtggtcatc aggatcggac gaaaacgcaa cagacaagcc tggtagattg catcgcgcgg
    35761 cgacatgcct tgctcgcgct cagcagccag cgcgaagtcg atcatcatga tggcgttctt
    35821 cttcacaata ccgatcagca aaataatgcc gataatcgca atcacatcca gttcgctacc
    35881 ggcaatcatt aatgccagca gcgcgccgac ccctgcggtg ggtagcgtcg agagaatggt
    35941 gatcgggtga ataaagctct cgtacagaat gccgagcacg atatacatcg ccaccaccgc
    36001 cgcgacaatc agccagacag tgctgcccag cgccgactgg aaggcgaggg tgctgccctg
    36061 gaattgcgtg gtgatatcca ccggcagatt cagcgtcttt tcggtgtcca taatcgcctg
    36121 caccgcatcg cccagcgaat agttatccgg cacgttaaag gagatggttg ttaccgggaa
    36181 ctgatccaga tggttgatgg aaagcggcgc aaaacgctgc tcaattttgg caattgagct
    36241 taacggcacc acgccaccgt cgctgctggt caggcgaatg gtatccagcg ccgcgaggcc
    36301 tggggtattt tcggtattgt gctccagcac cacgcgatac tggttagcct gagtataaat
    36361 agtggaaatc agtcgctgac caaacgcgtt gtacagggcg ttatcgacgt ccgccatgct
    36421 gatccccaga cggctggcgc tgtcgcgatc aacattgaca tacgccacca gccctttgtc
    36481 ctgccagtcg ctggagacat cagaaagctg tggcagttgc tggagttttt ccatcaactg
    36541 tggcacccag gtactgagcg catccagtga cgtggcctgc aaggtaaact ggtactgggt
    36601 gcggctgacc tgagtatcga tggtcaggtc ctgcgttggt tgcaggaaga gatcgacgcc
    36661 cggcacttta tctaccgccg tttgcagacg ggcgatgact ttttgcaccc gatcatcacg
    36721 ttcatccaac ggtttgaggt tgatttgtaa acgtgcacta ttcagcgacg ggttagtgcc
    36781 atcaacgcca acaaatgagg tcaggctttg cactgccgga tcctgcaaaa tcacgtccgc
    36841 gacctggcgt tgtcgctggg ccatattggc aaaggagctg gattgcggtg cctgcaaagt
    36901 gccctgaata atgccgttgt cctgtaccgg gaagaaacct ttcggaatga acacccacag
    36961 cagcacgcta agcagcagcg tgctgagtgc cacgcttaag gtcagccacg gatgattcag
    37021 cactttcgcc agtccacgac cataggcggc gattatcctg tcgaacattt tttccgaggc
    37081 acgggagaag cggttctgtt tacgcaacga ctcctggctg agcatccgcg cgcacatcat
    37141 cggtgtcagg gtcagcgaca ccaccgctga gatcaaaatc gctaccgcca gggtaatagc
    37201 aaattcgcgg aacagtcgcc cgacgatatc gcccataaac agcagtggga tcaacaccgc
    37261 aatcagtgag aaggtcagcg agataatggt aaagccgatt tcacctgcgc ccttgagcgc
    37321 cgccgccaac ggtttttcgc ctttttcgat atagcgggaa atgttttcga tcaccacgat
    37381 ggcgtcatcg accacgaatc cggtggcgat agttaacgcc atcagtgtca ggttattgat
    37441 tgaaaaatcg agaaacacca taaccgcgaa agtgccgatt aacgacagcg gtacagcaac
    37501 accgggaatg atggtcgccg gaatattgcg caaaaacagg tagataatca tgactaccag
    37561 cgcgatagcc atcatcaatt caaactgagt atcatcgacg gatgcgcgga tattggtggt
    37621 gcgatcggaa agcactgtca ccttcaccga tttcggcaga ctctcagtga gctgtggcag
    37681 catctgccga atgctgtcgg cggtggagat aatgttagca ccgggctggc gctgaacatt
    37741 catcacaatg gcctgttctt tgttcgccca cgcgccgagc cagctgtttt ctgcaccttg
    37801 ctctacagtt gctacatcgc ccagacgaat tggcgcgccg ttctggtagg cgatgattag
    37861 ctggcgatac tcttcggcgg attgcatctg gtcgttcgcg gaaagcgtga ccgcacggga
    37921 agggccgtcg aggctacctt ttgccgagtt aacgttagcg ccggtaatgg cggtgcgcac
    37981 ggtttcgctg gtcaggccga gggcggcaat cgcctgagcg ttaagtttga cgcgaacagc
    38041 cggacgctga ccgccggaaa gcgtcaccag gccgacgccg gaaatctgcg agattttctg
    38101 cgcgacgcgg gtttccacca tatcttccac ttgcgtcatc ggcatggcgg ttgaggtgac
    38161 ggcgagcgtc atgatcggcg gatctgccgg gttcactttg ctgtaaaccg gcgggttagg
    38221 cagatcgctc ggcaacaagt tggtcgcagc gttaatcgcg gcctgcactt cctgctcggc
    38281 gacattgagc ggtaatgtta gctggaactg caaagtgata actgacgcac cgccggaact
    38341 ttgcgacgac atctgtttca ggccagacat ctgcccgaac tggcgttcga gcggcgcggt
    38401 aacggcagag gtcatgacat ccgggctggc acttgggtag agcgtgacca cctgaatggt
    38461 cggatagtcc acttccggca gcgccgaaac gggcagggcg cgataaccga taatctcggc
    38521 gagtaagatc gccaccatca gcagcgtggt ggcgacagga cgcataataa acaggcgcga
    38581 cgggccgcct gtgctgctcg ggggtaacac ctgcatcagg agcgtgctcc ttttttcgcg
    38641 tattcgcggc tggtggcttt ctcttccgga gtggtggcgc tctgggcttc caccacttcc
    38701 actttctgac tgtcctgaat gcccggcgtc accagatgtt tgctgacctt gttttcgcta
    38761 ttcagcaccc agacaaaatg gccttcattg cccatttgca gggcggctgt tgggatcact
    38821 acggcgtttt gttcggtgtc gactaacatg cgcgcgttaa caaactgatt gggaaacagc
    38881 gcatcatcct gattattaaa gcgtgctttc actttaatcg taccggttgt ggcatcgatt
    38941 tggttatcga ggcttaacag cgtgccttca cttaatttct tcgaattggt gcgatcccag
    39001 gcttctacca tcagcggttt tccggctttt tgcgcctgca ctacagtagc gatatcgctt
    39061 tccggcaggg taaagactaa atcgatagga tgcgtctggg tgatcaccac aattccggtg
    39121 gtatcaccac tggagatttg gttaccaaca tcaacttgct tgagaccaac gcgaccatcg
    39181 actggtgcgg tgatgcggct ccagtcgagt tgcaactgcg cgctggcgac gcttgcttca
    39241 tcagccttaa tggtgccttc ggtttcactg accagcgcct gttgggcatc cagctcctga
    39301 cgggagacga gattggtttt tgccagttgc tgataacgcg ccagatcacg gcgggcgtta
    39361 gcaagcgtgg ctttatcttt tgccagttgg ccctgggttt gtgctaatgc aactttgaac
    39421 tggctggggt caatttctgc cagtaaatcg cctgctttga cctgctggcc ttcctggaaa
    39481 tgtaacgcca tcagttgacc gtccacgcgg ctgcgcaccg taacagtatt agcggcggta
    39541 atggtgccaa gcccggt
//
LOCUS       DADWTH010000028        38755 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.028, whole genome shotgun sequence.
ACCESSION   DADWTH010000028 DADWTH010000000
VERSION     DADWTH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.028"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNY02_001538"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNY02_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBB9433125.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="JNY02_001539"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="JNY02_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBB9433126.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    556..679
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="JNY02_001540"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="JNY02_001540"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBB9433127.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="JNY02_001541"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="JNY02_001541"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBB9433128.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="JNY02_001542"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="JNY02_001542"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBB9433129.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="JNY02_001543"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="JNY02_001543"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBB9433130.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="JNY02_001544"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="JNY02_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBB9433131.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="JNY02_001545"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="JNY02_001545"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBB9433132.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="JNY02_001546"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="JNY02_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBB9433133.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /locus_tag="JNY02_001547"
     CDS             7148..7759
                     /locus_tag="JNY02_001547"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBB9433134.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNY02_001548"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNY02_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBB9433135.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNY02_001549"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNY02_001549"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBB9433136.1"
                     /translation="MKITISGTSYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNY02_001550"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNY02_001550"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBB9433137.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12358)
                     /locus_tag="JNY02_001551"
     CDS             complement(11999..12358)
                     /locus_tag="JNY02_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433138.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(13008..13571)
                     /locus_tag="JNY02_001552"
     CDS             complement(13008..13571)
                     /locus_tag="JNY02_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433139.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13779..14717)
                     /locus_tag="JNY02_001553"
                     /pseudo
     CDS             complement(13779..14717)
                     /locus_tag="JNY02_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14780..15154)
                     /locus_tag="JNY02_001554"
     CDS             complement(14780..15154)
                     /locus_tag="JNY02_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBB9433140.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15158..16306)
                     /gene="wzy"
                     /locus_tag="JNY02_001555"
     CDS             complement(15158..16306)
                     /gene="wzy"
                     /locus_tag="JNY02_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBB9433141.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16326..17231)
                     /gene="rfbG"
                     /locus_tag="JNY02_001556"
     CDS             complement(16326..17231)
                     /gene="rfbG"
                     /locus_tag="JNY02_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBB9433142.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17224..18114)
                     /locus_tag="JNY02_001557"
     CDS             complement(17224..18114)
                     /locus_tag="JNY02_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBB9433143.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18144..19400)
                     /gene="wzx"
                     /locus_tag="JNY02_001558"
     CDS             complement(18144..19400)
                     /gene="wzx"
                     /locus_tag="JNY02_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBB9433144.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19416..19961)
                     /gene="rfbC"
                     /locus_tag="JNY02_001559"
     CDS             complement(19416..19961)
                     /gene="rfbC"
                     /locus_tag="JNY02_001559"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBB9433145.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19966..20844)
                     /gene="rfbA"
                     /locus_tag="JNY02_001560"
     CDS             complement(19966..20844)
                     /gene="rfbA"
                     /locus_tag="JNY02_001560"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBB9433146.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20903..21802)
                     /gene="rfbD"
                     /locus_tag="JNY02_001561"
     CDS             complement(20903..21802)
                     /gene="rfbD"
                     /locus_tag="JNY02_001561"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBB9433147.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21802..22887)
                     /gene="rfbB"
                     /locus_tag="JNY02_001562"
     CDS             complement(21802..22887)
                     /gene="rfbB"
                     /locus_tag="JNY02_001562"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBB9433148.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23260..24153)
                     /gene="galF"
                     /locus_tag="JNY02_001563"
     CDS             complement(23260..24153)
                     /gene="galF"
                     /locus_tag="JNY02_001563"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBB9433149.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24328..25722)
                     /gene="wcaM"
                     /locus_tag="JNY02_001564"
     CDS             complement(24328..25722)
                     /gene="wcaM"
                     /locus_tag="JNY02_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBB9433150.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25733..26953)
                     /gene="wcaL"
                     /locus_tag="JNY02_001565"
     CDS             complement(25733..26953)
                     /gene="wcaL"
                     /locus_tag="JNY02_001565"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBB9433151.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26950..28230)
                     /gene="wcaK"
                     /locus_tag="JNY02_001566"
     CDS             complement(26950..28230)
                     /gene="wcaK"
                     /locus_tag="JNY02_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBB9433152.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28410..29888)
                     /gene="wzxC"
                     /locus_tag="JNY02_001567"
     CDS             complement(28410..29888)
                     /gene="wzxC"
                     /locus_tag="JNY02_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBB9433153.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29890..31284)
                     /gene="wcaJ"
                     /locus_tag="JNY02_001568"
     CDS             complement(29890..31284)
                     /gene="wcaJ"
                     /locus_tag="JNY02_001568"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBB9433154.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31339..32709)
                     /gene="cpsG"
                     /locus_tag="JNY02_001569"
                     /gene_synonym="manB"
     CDS             complement(31339..32709)
                     /gene="cpsG"
                     /locus_tag="JNY02_001569"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBB9433155.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32814..34250)
                     /gene="cpsB"
                     /locus_tag="JNY02_001570"
     CDS             complement(32814..34250)
                     /gene="cpsB"
                     /locus_tag="JNY02_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBB9433156.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34253..35476)
                     /gene="wcaI"
                     /locus_tag="JNY02_001571"
                     /pseudo
     CDS             complement(34253..35476)
                     /gene="wcaI"
                     /locus_tag="JNY02_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(35473..35952)
                     /gene="gmm"
                     /locus_tag="JNY02_001572"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35473..35952)
                     /gene="gmm"
                     /locus_tag="JNY02_001572"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBB9433157.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35952..36920)
                     /gene="fcl"
                     /locus_tag="JNY02_001573"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35952..36920)
                     /gene="fcl"
                     /locus_tag="JNY02_001573"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBB9433158.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENSQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36923..38044)
                     /gene="gmd"
                     /locus_tag="JNY02_001574"
     CDS             complement(36923..38044)
                     /gene="gmd"
                     /locus_tag="JNY02_001574"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBB9433159.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38070..38618)
                     /gene="wcaF"
                     /locus_tag="JNY02_001575"
     CDS             complement(38070..38618)
                     /gene="wcaF"
                     /locus_tag="JNY02_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBB9433160.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38634..>38755)
                     /locus_tag="JNY02_001576"
     CDS             complement(38634..>38755)
                     /locus_tag="JNY02_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBB9433161.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10346 a         9697 c         8725 g         9987 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac
     3661 ccgctctcga cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcagg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgtgctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagct agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg ggccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtga tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcagaata aaaatatttg aattcaaaat gcaacgatcg ccccgaaaaa aaaataatac
    12421 acacaaggat aaaatatcgt cccaacttgt aatttgtaat gttgatatct tttattaaac
    12481 ttgcaattga gatcgcaacc agcaatactg acaatgggta aatgggtgat aaatacctgt
    12541 caccttgcgt aacagaaaac caaaataaga catatgttaa aacaatcgag aaaaataccc
    12601 aaagtatttt attattccta agtgtaaaga cagacagtat agcataaaaa acaacatcac
    12661 tgccaacttt attaattgct tgttgcaaat ctaatgtggc aggacttata ccatgtcgag
    12721 cttgttctgc tgtttgaact tgtagatcaa tttcattcca taggtagtga ccaaatatat
    12781 ttcctccagc aggactaaaa ggatcaccaa agatccagaa actacggaca taccaataaa
    12841 tgccaccaag aattgttgcc aagctaaaaa taagaaatgt ctgtatgttt ctctttttcg
    12901 ctatcaatat aacaaatatg atggctccat atatggcacc aaatagtttt acgccacaag
    12961 caattccaat aaaaaaacca cataaaaaaa ttaattcatt ttgtgtttta ttgttttctg
    13021 agcgaatata tataagaata ctggctgcag caaaacaaag atagcgttaa atcaatatat
    13081 gcataaccaa gatgatcttt aagtggattg atagtaaata gtaggattgt agcaataatg
    13141 ctaggataat aagttttcgt aatttcccat gaacagccaa caagtccaca ggctattaca
    13201 aaaaaacaga tatttgctaa aaactgagct aaccttacat ctcctaactg taaacctaaa
    13261 gccattaaaa gatctgcatt ttgcgggaac agaggaaaac gcaaatattg ttcaacaaca
    13321 attgcatggt gctcaactaa tgaacgagcc aaaggtaagt gatacatagt atcatcccaa
    13381 tcaccgggag ctcgcaggga cgataacaat gtaaccacaa aaatcaaaac aagaaataaa
    13441 ataacaaaca gcgtactgaa ctcaatatta tttattgcca gccatctaaa ttgctgacgt
    13501 ttaataatgc ttaaaaccca acacaccgta aaaattaaaa agatagcaat cttattaaat
    13561 tgcccgacca tgcccagaaa gtaaattatc aaaatattta aagacatccc tatgcatagg
    13621 caataaaaaa ataaagaaaa tttgttaagt cctgccaccc cctccttaaa aaaagatgac
    13681 aatacaatat taccaatgac aaaaaatgtc attccaaaag caaaacaata cacaacatac
    13741 aaatacaacg gcaattcaat catagtaaaa tactctattt acattatcag tttatagttt
    13801 ttttcagaaa gataaaataa tgccttcatt tcacgtctag ttgttgtatt tgaatgcaaa
    13861 ataatgccac atacaaatgc cattattgac gataacatga tggaggcagt taaaaaggca
    13921 gtcggaaatc ttctcaccaa acctgtatca atataatcaa caatgatggg caatcctaaa
    13981 attatagaca aagcagcaag taaaacgccg ataatactaa aaaagtatag tggccgttca
    14041 agcgagtaaa gtttaattat tgttttcaga atcttgaacc catcactcca agttgatagt
    14101 ttgctcacac tcccttcaga tcgctcgcca tattttgtat taacctcacc atacttcata
    14161 cgtaattcta aagcatgaat tgtaagctca gtttcgattt caaatcccct tgatagacat
    14221 ggaaaagatt taacatagcg tctggaaaat acacgatacc ccgacaacat atcagtaaaa
    14281 ttccctttaa agatagtctg aacagtacca gtcaacagtt tatttcctca gcgatgccct
    14341 tttctgtatg tattttgatc tccattctct tgacgacatc ctacaaccat atccaattga
    14401 ttttttatga gatggttaat tatttctgga gcagaacttg catcataagt gccatctcca
    14461 tcaaccatta gataaatatc cgcatctaca tctgagaaca tacgtcttac gacattcccc
    14521 ttgcctttca gaggaacaga gtgaactgtt gctccagcat cctctgccac ttgtgctgta
    14581 ctgtcagtcg aattattatc aaatacatat atagaagcag ttggaatatt cgttttgaaa
    14641 tcatttatga cttttgctat tgccttctgc tcattataac aaggaataat tacagcgata
    14701 ttaaaagagt gatacataag cccctcatat aaactattta gctaatgtgc aagtaattgt
    14761 gtttttaaca aatcgtttac taacgaaaaa tgaaatattt tgatgcgcaa tatcctaaaa
    14821 ccaaactaaa aagagaaaag gtgaacaatg taaatattgg agataaagct aagagatcaa
    14881 ataaaacgcc gaataaataa ctcatcgccc ccataaaaaa tataaatata aaatatctag
    14941 ccttggttgc agatacttta aatgagcatc ttgcatttgc aaaaaaagaa aaggttgctg
    15001 cacatataaa accaacaatg ttacttcggc cttgaccaaa agctaaatta ttataacacg
    15061 cataaaatac cccccaatgt aaagcagtat tgacaattcc aattaaaaaa taactaaaaa
    15121 aagaactggt caataacttc cctattttta acatccttta ttttgctcca gaagtgaggt
    15181 tattactaat ttggatattt tctatagaaa atacccataa aactacaacg cctataataa
    15241 aaattcccat gggtgtaaaa agaaatggat tagttataga aacggtaata ctgaaaaaaa
    15301 gtattaacga catcattaat gatgtttttg tactatttaa aaaatagcct ttcaaaaaga
    15361 taagcaacat aggaactaat gatataaata caccaataac gcctgttttc tgaagtatct
    15421 caagaggtac tatttcaatc cttaatcgat ctaatataaa tgaaccaaat cctcttccaa
    15481 acaagaacgt cgctaaatca acatttttat aataaaaata taaatcatta aatctaacag
    15541 agtcagaatc ttcactgcgt gatccgacca tgtaaagcat aattataaat agaacgctgc
    15601 ccaatattat tataatagta tttaaatata gcttatgtaa tttaaaactg gctaataaca
    15661 gtgataatgt tgtaaaaagt aatacacctc ttgattctgt caataatatc gcaagcacta
    15721 ttatgagaca ttttaaatat tttttgtcga caaaactgat aatcgcaccg acaccaaaaa
    15781 agtagaagga tttggaaaaa aaagccccat cgggacgaaa aaagaactcg ctagtcaatg
    15841 ataagtgttc ataaattaaa ttaaaattta acaaaccgaa attcaacaaa attatgtatg
    15901 aaatatataa cagtgatcca tataatgcaa atgcattaac tattttaata aaaatctcac
    15961 ataaaaatac ttttaaagta tatcttagtg taaatagata tataagtgac agcataaaaa
    16021 ggtatggttg cacatcaaac agtatatcat ttattttatt accagctaat aagccgacaa
    16081 agaagccata aaaaggaaat aaaagcaacc atataaaaag actgacacat ttttgggtga
    16141 ttgaaaaatt gaatgtaact aaattaaaaa ttaaaagtat aaaactaaat acaaataata
    16201 aatatcgcaa agggaatatt cccaatggct ccagtaatct tccaccacca ccaataatca
    16261 gttcaataca taaaaatgtt ataaaaattt tatttatatt attcatattc gtaaggtgat
    16321 gttttttaaa atatgtttaa aatcaacatt aataaaatat aaattttatc agcctttttc
    16381 cgccttaact tccaaaaact taaagccaag atcaaattgc ctatataacc tttacccaat
    16441 tgattattca caaagctatc atcagctaag gcattagcta ttttagttac ttcttttcga
    16501 taccacttgt ttctcaccaa accaagccgt ttgtattttg ctttaaaact aatgtttgct
    16561 cccacttggt tgttttcatg ctgtctgtac agcattgttg gttttctatc aatataccac
    16621 gaataatttc tcgtccgcgc aaatgaatat aaaaaccaat catgcagaca gatatcttgt
    16681 gcagcatttt tattatttat gatgaacttc ttaaattcaa taagcgtttc tttttttata
    16741 acatatgtac accccggacc tgctgcttca aaaaaataat cgaattgagt ttgcgactga
    16801 gccttatcaa ctaaattttt ttgccctgat ggataatatg caattacatt gctggaataa
    16861 caattcgcat tattaaatac caagcagtca attgcatttt ttattttatc tttataccaa
    16921 atatcgtcct gatcactaaa acagatataa tcatagtttt caagatcgac atctttaatt
    16981 aatctataaa aatttttacc tgccccccca aacttattga cgcttggaag tatatttata
    17041 attgagggat atgataattg aaattcttca caaatattta atgtattatc agttgataaa
    17101 tcatctgata taaaaattga gatgtctaca tctttttgtt caataatact ttttaattgt
    17161 tcctcaatcc agcattcgcc attatacgta gccataagta cagctactct tttttgatca
    17221 gaactatgca ttataacgac cgcccccagt aattcctctt attccatcca gcgtagccaa
    17281 aatatgtttt ttcatatatt caattttatt tttttgagtt gcgataatta aagtcgtatg
    17341 aattaaagaa aaaacaactt ccctaataga gagtaaaaga ggcacatgat tttttctaag
    17401 caaaagaaat gcatttctta ctcgataata acgacgtact ggcgagtgaa ttggtatgtt
    17461 aatcccacag atttttactg acttatcccc aatagcatga accatttcta tatcaggaat
    17521 gatatgaact aatatgccat agtttaagca tctaagacac cattctgtat cgacataatc
    17581 aataaaaagt tcctcatcca tcattccaac aatctcaaaa acttcttttg aaaccatggt
    17641 tccggatgag attgttactg aacttttaaa cggtgtctgt ccttcagtga catgaatttt
    17701 ctctcgaaga ccattttttt tgatattaca aataggataa taatgtgact tatctctatc
    17761 aataaaaact gggccagcta tttttgcatt ttcattatcc atatagcagg aaagtttttt
    17821 gataaactca ttgcgaatac ggctatcttg atcaaaaaaa attatcttct ctgccccttt
    17881 ttcaagagaa tatcgaatcc caatattttg tgcttttgct ataccttcat tcctgccaag
    17941 acaaattaaa tgaacttttt caatgtctgc taatttatct ttcaaggtga aattagtcct
    18001 attatcgact actacaacgt aacaattttg ctcctttagt tcagtgatca gtgcattcag
    18061 atttttaagt tcgggattat atgtcacaat gacagcgtaa atattactat tcattatcca
    18121 agtgactcag taattggtta attttagttt tcatatacag aacacattct agtataaagc
    18181 cattgtaata acagagcatc taaaattaca cgtatgctcc aggatattgc ggcacccagt
    18241 aacccatatt tttcgaccat aaaatacaaa agggctaaat aaggaaccaa ttcagcacaa
    18301 tgaatcaaag cagtaatttt agattttcct aatgcctgga ttgcagagaa tggtatcatt
    18361 gccagcgcat tgaagaaaaa accgatcaat aacacattta atatattaaa ggaattaatt
    18421 tggtaattct caccaaacca tattttcaat acaagccctg aatataacaa gcctatgatt
    18481 atcaccggca aacagattag aaacattaaa agcaatgatt tatttacatt acgtttaaat
    18541 tctttaaagt ctttgatgtt acttaacctt ggaaacactg ccctcccgat tgctgcaggt
    18601 attattccta attttagaat aacctccgct ggtgcagaat aaaatgcaac tttatctgcc
    18661 cccataatat ttgacactat aaatcgatca aaatacacca tgaccggact tatgatatta
    18721 cttactgtca tccaaccgcc aaagaaaaag agtcgtttaa aggttttata acaaaaatga
    18781 acccccgaaa ttttaatatc attcctgaca taataggcag agactaaaat cgaaataact
    18841 cttgcaaata ttaaaccagc aaccgctgcc gacaatgtag cactataaaa aacaaatatg
    18901 gccggaattc ccgcgatgca agagcttgat atagattttt gaatatttac aatgccaaat
    18961 ttttcatccc cctcaagaat ggctgaccat aattgattta gaataaataa tggaatgcaa
    19021 atagccaaca gtttaaatgc tagttgtata tcactatgtt caacaccaga aatttttaga
    19081 taattaacaa ttccatcaga gaaaatcaat aataaaaaag caccaaagca tgaaaatagc
    19141 accaaaaaag atgtacttgt tgaaattacc ttggttcttt catgatgatt atcacgatga
    19201 attgcaattt ctctaataac agagcgagtc aggcctacat caaaaattcc agcataccca
    19261 acaagtgcaa ttgcaattgt ataaacaccg aaaccttcag gccccaaacc tcgagcgaga
    19321 aatcctagcg aggggatagc aattaaagtt ggtattgcat agccaaaaag gttccagaca
    19381 ctatttttta ttatgctcat tattacattt ctttatcata taaaataatc agcatctaca
    19441 aatttttgac cattaatatc tttatcagaa agcaatggat tttgaaccgg ccattctata
    19501 tttatacttt tgtcgctcca taaaatccca cgctcttgca tttttgaata gaaattattt
    19561 gttttgtaga caaattctgc ttcatcacta agaacgcaaa acccatgagc gaatccttcc
    19621 ggtatccata attgacgctt attctccgcc gacaaattta caccaaccca cttgccaaat
    19681 gttggtgatg atttccgaat atcaacagct acgtcaaaaa cttcaccgac aacacagcgt
    19741 accagttttc cttgagcata aggttccagc tgatagtgca gtccgcgtaa cacaccctta
    19801 gttgatttag aatggttatc ctgaacaaac tcaacctttc gtcctacagc ctcttcgaaa
    19861 actttctgat tgaagctttc cataaaaaag ccccgctcat caccaaatac ttttggttca
    19921 aaaattagca catcaggaat ttcagtttta attacgttca ttttattaat aacctttaat
    19981 cattttcagc agatactgac cataagcatt tttcttcagc ggctcggcta ataccttcac
    20041 ttgctcagca tcaataaagc ctttacggta agcaatttct tccgggcagg aaactttcag
    20101 cccctgacgc tcttcaattg ttgcaatgaa gttgcttgct tcaataagac tttgatgtgt
    20161 ccccgtgtcc agccatgcat aaccacgccc catcatagcg acagacaaac gcccttggtc
    20221 catataaata cggttaatat cggtaatttc cagttcgcca cgtgcagatg gtttaaggtt
    20281 tttcgccatt tctacaacgt cattgtcata gaaataaagc ccagtaaccg catagttact
    20341 ttttggttcc agcggttttt cttccagact aattgccgta ccgttatcat caaattccac
    20401 cacaccgtag cgctcaggat catttacgtg ataggcaaaa accgttgcgc cactctctct
    20461 gttaacagcg gtatccatta atttcggcag gtcgtgacca tagaagatat tatcaccaag
    20521 taccagcgca caatcatcac cacctataaa ttcttcacca atgataaatg cttgcgccag
    20581 accatccgga ctcggttgta ctttatattg cagattaagc ccccactggc ttccatcacc
    20641 aagcaattgt tgaaaacgtg gtgtatcctg cggcgtactg ataatcagaa tatctcgaat
    20701 acccgccaac ataagcgttg aaagcggata ataaatcatc ggtttatcat aaatcggtag
    20761 cagttgtttg ctcacagcca ttgtcacagg ataaagacga gtgccggaac caccagccag
    20821 aataatacct ttacgcgttt tcatttcact attcctttta attcatctcg ctctggcatc
    20881 atgagcgaga tgcaataatt tgttaaattg ccgtggtcgt aaataattcg ttgagcatac
    20941 gtttcacgcc cacctgccag tcaggcaaga caagcgcaaa gttctgctga aatttttcgg
    21001 tattgaggcg agaattatgg ggacgacgtg ctggtgtggg ataggccgtt gtcggcacgg
    21061 cattaagttt gttaagagca aggttgatcc ccgctctgcg cgcttcttca aataccagag
    21121 cggcataatc gtgccaggtt gttgtaccac cagcaaccag atggtacaaa ccagcaactt
    21181 ctggtttatt tgcggccacg cgaatggcat gagcggtgca atcagccagt aattcagccc
    21241 ccgttggtgc gccgaattga tcgtttatta cagccaactc ttcgcgttct tttgccaggc
    21301 gcaacatcgt tttggcaaag ttatttcctt taccagcgta tacccagctg gtacggaaaa
    21361 taagatgctt tgcgcaatgt tcttgtaacg ctttttcccc agctagcttg gtttcaccgt
    21421 agacatttag cggtgcggtt gcatccgttt ccagccatgg cgtgtcaccg ttgcctggga
    21481 aaacataatc agtggagtag tgtataaccc aggctccgac ttcattagct gcttttgcaa
    21541 tcgcttcgac gcatgtcgcg ttaagtaatt gcgcgaaatt cggttctgat tccgctttgt
    21601 ctactgcagt gtgagcagca gcattaacaa taacatcggg gcgaattttt ttgacggttt
    21661 cagccacacc ttcggggttg ctgaaatctc cacaataatc agtggagtga acatcgagcg
    21721 caatcaagtt acccagagga gccagagcac gctgcagttc ccaacccacc tgccctgttt
    21781 tgccgaaaag caggatattc attactgacg gccctcatag ttttgttcaa tccacgactg
    21841 ataagcaccg cttttcacat tctcaaccca atttgtatta gccagatacc attccaccgt
    21901 tttacgaatc ccgctctcaa acgtttcttg cggtttccag cccaattcgc ggctaatttt
    21961 atcggcatca atggcgtaac ggcggtcatg ccccgggcga tcggcaacat aagtaatttg
    22021 ctcacgataa gatttctctt tcggtacaat ctcatccaac aaatcacaaa tagtgagcac
    22081 tacgtcgatg tttttctttt cgttatgtcc accaatgtta taagtttcac ccgctttacc
    22141 ttcggttacg acggtatata acgcacgagc atgatcctct acatacaacc agtcgcgaat
    22201 ttgatcccct ttaccataaa taggtaatgc cttaccttcc agagcattca gaataaccaa
    22261 tggaataagt ttttccggga agtgataagg accataattg ttcgagcaat tagtcacaat
    22321 ggttggtaaa ccataagtcc gcttccaggc acgtaccaaa tgatcgctgg aagctttaga
    22381 cgcagaatac gggctacttg gcgcgtaagc tgtcgtttcc gtaaatagcg gtaacgcttc
    22441 gttattattt gcttcatccg gatggggtaa gtcaccataa acttcatcag tagaaatatg
    22501 atgaaagcgg aaactttttt tcttttcatc attcagagca gaccagtaat tgcgcgccgc
    22561 ttctaaaaga acataagtcc ccacaatatt ggtttcaata aatgccgcag ggcccgttat
    22621 tgaacggtca acgtggctct ctgccgccag gtgcatcacc gcgtctggct ggtgctgtgc
    22681 gaaaatacgc gacatggcta cagcatcgca gatatctgca tgctcaaaag cataacgttc
    22741 agaatcagaa acatcagcga gtgattctag gttcccggcg tatgttaatt tatcgacatt
    22801 aacaacacta tcttgcgtat tatttattat gtgacgaaca acagcagaac caataaaccc
    22861 tgcgccacca gtaacaagta tcttcacttt tttattccat atagctatag agcatgctgt
    22921 gaaatagact gctccagatt tgattaatag aggtattaat acacgctacc gcccctggct
    22981 taacagctac cagtgcactg catgcatctc aacgatgtgg cgagcgttag ccactcgcgc
    23041 caaatccgaa aaattcaaac gttaattgtc ttaccaaaca gccgtagaaa caaggaaaat
    23101 cctggaaaaa tttgaataat atgagctatg taacttcttg tttttatagg ctttataaaa
    23161 acggcaacgg caggaaaatt caatgagcaa aacttatgcc acgaatcttc actgccgtta
    23221 taattttctt atcaaccgtt acatccggtc agattttcat tattcgctta acagcttctc
    23281 aataccttta cggaacttcg ccccttcttt caggttgcgt aggccatact tcacaaacgc
    23341 ctgcatatag cccatttttt tgccgcagtc gtaactgtcg ccggtcatca gcattgcatc
    23401 aacggattgt tttttcgcca gctcggcaat agcatcagtc agctgaatac gtccccatgc
    23461 accaggctga gtacgttcca gttccggcca aatatcggca gaaagcacat agcgacctac
    23521 ggccatgatg tctgagtcca gcgtctgcgg ctgatccggt ttttcgataa attcaacaat
    23581 acggctgact ttaccctcac ggtccagcgg ctctttagtc tggatgacgg agtattcaga
    23641 gaggtcaccc ggcatacgtt ttgccagcac ctggctgcgg cccgtttcgt tgaaacgtgc
    23701 aatcatggca gcaaggttgt aacgtagcgg gtcggcgctg gcatcgtcga tcacaacgtc
    23761 tggcagtacc acgacaaatg ggttgtcacc aatggcaggt cgcgcacaca aaatggagtg
    23821 gcctaaacct aaaggttcgc cctgacgcac gttcataatg gtcacgcccg gcggacagat
    23881 ggactgtact tccgccagca gttgacgctt cacgcgctgc tcaaggagtg actctaactc
    23941 ataagaggtg tcgaagtggt tttcgaccgc gttcttggac gcgtgagtta ccaggaggat
    24001 ttctttgatc cctgcagcca caatctcgtc aacaatgtac tgaatcattg gcttgtcgac
    24061 gattggtagc atctctttgg gtatcgcctt agtggcaggc aacatatgca tcccgagacc
    24121 cgctacagga ataactgctt ttaaattcgt cattatttca tccacctgta aaatggttgc
    24181 tgaattatag cttgttcgat ttttttcgcc agcatcaatt accctgaatt gattactgaa
    24241 ttacttgtga tgttacgccg cttcgttgtg gattgcagta gcattgttcc taagtatggc
    24301 tccatttttc caggaatggt cgcaaatcta ctccctccgt tccggcaatc taaagttaat
    24361 cttttccaca ttaacaatat ggtgattaat cctgtcgata tcgacggagc tttgccctct
    24421 ttcattcacc gcatgaacat ttgcaagaga cagcagtgtt tcttttttcg ccataaagac
    24481 gccgcgaacg tctttgcgca tgtcgaagtt catgctcaat gctggtccaa ctgaggattc
    24541 ctgcatcaca ttgatatttc gtataaaaag atgttgcggc ttgttgtgta actccagcga
    24601 ggcacgcttc atctcaatgt tagttagcga cacaaaggag acggcattcc cggcggagat
    24661 ttggatgccg cgcaatttat aagcaagatg ggtattatcc agttgaatat tattcactcg
    24721 gaaattttgc ggtatcgaga gatatttgcc tttaattacc ccatagccga ttaacatccc
    24781 ggcgctatta atcatttcaa tattatcaat cacgaaattg tcacaaccgt aaatagcgac
    24841 tgtcgcgtta tcaatgcccg ctttcttact gaaatccggc gtgatattgc gggcattgat
    24901 attacgaata acaaaatgtt taccattttc aacatggttc aactgccgac aatccgatcc
    24961 cgtgatattc gccacgacaa agtttttcac tgcctggtct tccgggtagt tgttatcata
    25021 agtgcttccc gcaaggccta tgccgatgcc ccagttgatt ttgccgttgg tacagttgat
    25081 acgctcgatg acatggtcag atatcaaaat atcactgtcg ttaattgcca cgttccattc
    25141 aatggcgtcg ccctgtaagt cgctgaactt acagttggta atgttggcac cgataatctg
    25201 gttatgaaat ccctggcgta agatggcgta attagcgtgg ctgacagtga ggttatcgat
    25261 ggtcaggttg cgcatgaccc gtttgttttt accgccaata taaatctgca ttaccgggcc
    25321 aaagccgctc atcgccagcc ctttgatggt gcagtcagaa ccacgcacat ccagggtaat
    25381 gttatgcata ccgccgccct ctccccctgt cacctggctg ccgtcctgta agacaaatcg
    25441 ccctctgccg ttgccgcgca ggcttccaag gatgtgtaac gttttgccgg gagggatgaa
    25501 gatgccggtg ttgatattgt cacaaacgaa tccggcaggc acgacgaccg tttgcccttc
    25561 gctgaaggct tgtttaaatg aggcgatcca gtcatgtggg ttgtagtcgt taatgttaac
    25621 gctttgtcgg gcgggaagtg cgcgagcgaa aggggtatgg aggaaggcaa gcgccgagct
    25681 tgccgtcagg aaggtgcgtc gggagagtgt tttaaatggc atacgttctc ctctataaag
    25741 cctgcagcaa gctggcgagt tctcgattaa tcacctgctg gttaaaatcg tgttcgactt
    25801 tttcgcgcgc acgtttgacg accggagcca gttcgtcggt atccagttgg ctaaacgccg
    25861 ccagacgttg tgccagtgcg cgagcatcgt tctcaggcac cagccagccg gatttatcgg
    25921 cctccaccag ttcaggaatt ccgctatgca gagtagaaac caccggaata ccgaccgcca
    25981 tcgcttccat tagcgccacc ggaatgcctt ccatatcgcc atccgcgccc gttaccgatg
    26041 gcaacaggaa gacatccgcg tcgtcgagca tctccttcac ttcatggctc ggtttaaagc
    26101 ccggcatctc taccacatct tccagttgat attgttcgat gagggtgcgc aggcgtcgtt
    26161 cccacaggcc aatgccgagg atgcgatagc gaaatgccac gccctgctct ttcaactgac
    26221 ggcaggcttc aatcgccaca tgcaggcctt ttttctcggt taagcgtgcg acagatatga
    26281 tttccagcgg cgttgcgggc gctttcaccg gacgcgggct aaagcgcgtc atgtccacgc
    26341 ccatacgcga tacggcgatt ttttctctgg ggcagcccat tttttgcagc cttccggccc
    26401 acagatcgct tatcggtaac atcaggtcgc cacggcgaaa cagttgttga tattcgggag
    26461 tgtagtggtt gagcacttcc cgactggaga tatcaatacc gtggaagatg gtggcaattt
    26521 tgccgcgaat gatacccagt tcgcgtagtt ttgctgcggt tacccccgcc gggccaaaat
    26581 gagcgatgaa cacatcggcg cgaaacggtg ttgcgacctg accacaaatg gcagacaaaa
    26641 tcaggttccg cgactcggca ccatagcgtt tgaggttgag cgcatgccag gtatttttac
    26701 gatgaatgcc acgtaaggtc tggctggcgc ggtggcgcag tttcgctact ttgcccgcag
    26761 gttcgtcctg taaccagcgg gttctggcga caaggttgta tttcgtccat gccgcgtggg
    26821 tgttctctgt atcgcctttt tgcaaagcga caatctccac ttcaaagccc atatcaataa
    26881 acgcggtaat ttgattgagg acgaaggttt ccgacgacag cgggaatttc aataagaaga
    26941 agccgacctt catttcacct ccccgatgcg ctcaagcaca gactgcacca tctgcattcc
    27001 cgtctgacgt tcgcgactga cggcttcgtt aaatcgagcg ttcagcaccg gaagctggcc
    27061 taaggtatcc gcaaccatcg cttgcaggct gccgtccagt aaatgacgga tatcaatcgc
    27121 catctctggc agtcccagct gctgcataat cccggcggat ttatgttcat agttgatggc
    27181 aatcgccgga gtagcaaagt tcattgagat aatggcggag tgcaggcgcg taccgacggt
    27241 gagttcacag gctccgagaa ttttgcccat ttccagatcg ttgagttcat ccatcactac
    27301 gtggtaacgg gcaggatcgc tgatgtgctg gcgcagattg agcgccacca tgcggtcgtc
    27361 tttgttatag ctgtcgatac ccgtacaggt ggagagcgca atcacctgat agccttcatc
    27421 gagaatgcga ttgaccaccc cggcaaaggc tttttcatac gcttgttgag tggtgccgag
    27481 acgtttgtcg aacggtgcca gttcgcgcag ggtaattgcc actgttttct gttgtgcggc
    27541 aacgtccagc cagtgttgaa cggcatagct ggcggtgaag tcttcggtgt ggtgatcgac
    27601 cagccacgcg gtatcgacgc catgttccac ttttgcggtg gtgatattgc tgcgtttcat
    27661 caaatcaagg ctgaccgatt cgcgcaggat cagcgcgtcg cagtgaccaa agacatagtt
    27721 cgccagttgg ttaaattgct catcctggaa ggggccgacg ctgtgaccaa tcataaacag
    27781 cggctttttc gccataaatg tgcaaagcgc atgttcaaac tgcggcacgc cgtagagatc
    27841 gacaaaaaac gatccgccga cctgaataat ggcgtcgtaa cctgacagca ggcgcacgaa
    27901 gtcggtgaat ccctgggcga tggcgatatt gcgcagcttg ccagtgtcgg tgacgcgtga
    27961 gagcaatacc tgatgctggt agcggcgacg gaggactttt ttaacgcgcc cgacaacgcc
    28021 cgccgcgcta ttgtgttgtg tcatctgcag gaacagcgga tcgcccatta ccgggcggtt
    28081 gagcagccag gaagaactga ccggatagcg gctcatcacg tccacttcgg tatgtggatt
    28141 gaggatgttg atggcatcaa gtaagccgcg caagatggcg ctgtcgccac gattgccgca
    28201 agtgtggttg cccagaataa gtaatttcat tttttcctca taaatttgat gccaggtgag
    28261 gctgtgtttg ttgtgttcgt tgccgttttt tgcctgatgc gacgctggcg cgtcttatca
    28321 ggcctacagg tcccgagcac agaaccgtag gacggataag gcgttttacg ccgcatccgg
    28381 caaccgttgt cggaaccgaa aacagcaatt caccccgccc gtaaaagcat tttcattttt
    28441 tcgctgcgac aaaactgacg cttcacttcc accaccagcg gatggcgtga cagcacaatc
    28501 atcaccacaa acgccagcac gcccgcggct atttgcaccg ccagcagcat ccccagcgcc
    28561 agttgccctt tcagcacaat gcccagcgca taactgacca ccagcgtcgg caacgagaga
    28621 taaaacggca gccacaaact caggatgtac tggcgataac tggaaccaag aaccggttta
    28681 atcatcacga agtaactcag aatggtgttg ataatctgca ccagcaggaa accgagcgtg
    28741 acgccgattg cgcccgccat ctgcccacca ataacaatcg ccggaataaa cagaaatgtt
    28801 ttgaatacat tgaatttaaa gctgatatcg acccgcgctt tcgccatcag cagcgaacca
    28861 atcgggttac ccacggagcg cagcagaccc acaatacaca gcaattgcag caccggaata
    28921 atgctgttcc acttttcgcc gaataccagc ggtacgaagt tattcgacac caccatcagc
    28981 cccagcagcg ccggaaagtt gataatcccc actatcgaca gcagcttgta gaagttaaca
    29041 cgcagctttt cggtgtcgtc ctgaattttg gcaaacgccg gaaacaacac gcgggtgatg
    29101 attgggttca gcttcatcgg cggtacgacg gccacgttgt acgccaggtt gtatccccct
    29161 gccacgcccg cgccaagaat acgcgccagc accagcgttg aaaggttggt gttgagatag
    29221 ttgatgatgc tgtccgccgt cagccaggca ccaaaacgta agttcggtgc cacagacgcc
    29281 agcgagaagt gcagaccggg gcaataaatt ttgcgtccaa agtagccaaa cagcagtgtt
    29341 ctcaccgcac tattgaccag ataaccgagg atcgcggtca tcgccagcgg ccagaaatgg
    29401 gcgctaacta ccgtaaaagt gaagcccgcc agcaccgcgc tggtttcgat catgccgatt
    29461 ttgttgaact ccagctcctt ctgcatcaac gcgcggaact gttgcccgtg ggggatcacc
    29521 acaaacgcca gcgacaatgt tttaatcaac ggtgccaggt ccgggttatt cagcacgtcg
    29581 ccgatgacat cactcaacaa aaacaccgcc acacatacca caagccccag cccgacgttc
    29641 agccagtaca gcgtggtgag ttcaaggtga ctgatttctt ttcgctgaat aatcgagtta
    29701 gcgataccga agtcagaaag cgtatctgcc agcgcgataa tcaccagcga cacagtcagc
    29761 aggccgaact ggtggttgtc gataatccgc gccagcacgg tcatctgcac cagcccgagg
    29821 ccgatgatga tcaccgtggc aatagccgac cacttcgcgc cgctgatggt tttttcacgt
    29881 aagctcatat caatatgccg ctttgttaac gaagcccttg aataccgtca ggaaaacgat
    29941 tttgatatcg aaccagacgc tccattcgcg gatgtactca aggtcgaact cgacgcgttt
    30001 ttccattttc tccagcgtgt cggtttcgcc gcgccagcca ttaatctgcg cccagccggt
    30061 aatgcccggt ttcactttat ggcgcagcat gtagccttca atgagctggc gatactgttc
    30121 gttatgcgct actgcgtgcg gacgtggacc gacaatcgac atccccccgg tcagcacatt
    30181 gataaactgc ggcaattcgt ccagcgaggt gcggcgcaga aagttcccca ctttagtgac
    30241 gcgcggatcg ttctgcgtcg cctgggtcac cactttgtcg ttctccatca ctttcatgga
    30301 acggaacttc cacactttga tcggcttgcc atccatacca tagcgagtct ggcggaaaat
    30361 aaccggccct ggtgaactga gtttcaccgc cagcgcaata cagcacagca ccggggagat
    30421 cagcagcaga ataagcgtcg ccagcacaat gtcttccgca cgttttagca ggcggttacc
    30481 ccccgaaagc ggcgtgtcat acagcggcac caccggaacg ccgttcatct cttcgagacg
    30541 tgaatggaga atgttgaagg taaagacgtc ggggatcagc agcaccgaac aggtggtatc
    30601 cgccagttga tgggccagtt ttttcactcg tgcgccgtcg cacatttgca tcgcgatata
    30661 gacgttatga atcttgcccg cttttgcatc ctcgaccagc tgttgcaggt tacccgccca
    30721 gtcgttagaa acgccgcccg gtttcgggtc gtggtaaacg cccaccactt caaaccctaa
    30781 ccacggctga ttacggaaac tctccatcag catttgtccg gcggctaaat cccccgctac
    30841 ggcgaccatc cgcttgttgt agccatgatt acgcagccag cccgccccaa tgcgaataca
    30901 tgaacggcaa accaccagtc cgatgctggt cagcccatac cacgccagcc agattttcag
    30961 ttgcgtgtcg aaatcattgt tgaacgccac cagtccggcg ctgaaaatca cgcttaaggt
    31021 ccagttttgt agcaacaggg caaattctgt cgctgcccga acaccgcgcc atgagcgata
    31081 aaaatcggtg atgccgccca gcatctggaa caccaccagc gtaatcagcg ccaccaacag
    31141 gtgcatgtag aggaatgaca gtccgctgac ttcgcaaacc agccatagcc cggcaaacat
    31201 gatggtgata tctgaaaagc gttgcaccat agagattaac gatgcattgg ttttcgctcg
    31261 ctcgcgcttt tttagatttg tcatcgttgt tcctgttatt agccccttac ccgcagtggg
    31321 gtaagggaag atccgacatt actcgttcag caacgtcagc agagttcgcg ttcgcgcttc
    31381 catcagcggc acatctccgc gcgattccac attcaggcgc accaccggtt cggtattgga
    31441 ggtgcgcagg ttaaagcgcc agtcggcaaa ggtcatgctg atgccatcgc tgcgatccac
    31501 cgccagcgcc tcacggctaa aatgctgttc cacgcggtta atcgcctcaa cgggttgcgc
    31561 cagtttgctg ttgatctcac cgcttgccgg aaacgccgcc atccggtcgc gcaccagttc
    31621 gcccagcgtt tttcctttca ggcacaccag ttcggcgacc agcagccacg ggatcatgcc
    31681 gctgtcgcag taagcgaaat cacggaagta atggtgggcg ctcatttcgc caccgtagat
    31741 ggcgtcttcc ttgcgcatcc gttctttaat aaaggcgtgt ccggttttcg acattaccgg
    31801 cgtgccgcct gcagcagtca ccacatcaat agtgttccag gagagacgcg gatcatggat
    31861 gatcttcgcg ccgggatttt tttcgaggaa cgcttctgcc agcaggccga caatgtagta
    31921 gccctcgata aactgccctt tttcgtcaaa cagaaaacag cggtcaaaat cgccatcaaa
    31981 ggcaatgccc atatccgcgc cgtgtttgat gaccgcattg cgggtgtcgt cgcgacattc
    32041 cggcagcagc gggttaggaa taccgttggg gaaattgccg tccggtgtgt tgtgtacttt
    32101 gattaattcc acgggcgcgc cgagggcttt aaagcgggct tcaatggcgt ccaccaccgg
    32161 acccgctgcg ccattcccgg agttgatcac cagcttgagc ggcgtgagat tcttgacatt
    32221 gatataaccg aacaggtgat caacgtaagc gtcacgcagg ttgatttgct gatagcgacc
    32281 gcgtttggtt tcatcgacgg gaggaaagtc gttggcttca gccagacgct ggacgtcgcg
    32341 cagtccggta tcaccgctga tcgggcgagc cccctcgcgc accagcttca tgccgttgta
    32401 atccatcgga ttatggctgg cagtaacttc aatgccgcca tccacgccga gatggaacgt
    32461 ggcgaaatag atctcttcgg tgccggacat accgatatcc agcacatcga cgcccgcatc
    32521 ctgtaaacct tttgccagcg ccagttttaa ggtttcgctg gtgagacgga catcaccgcc
    32581 taacacaatg gttttcggtt tgagaaattc gccataggcg cgaccaatgc gccaggcgat
    32641 atcttcattc agttcttcgc ctaatttccc gcgaatatca taggctttaa agcaggttaa
    32701 ttttttcatg tcgttaccct ttttcaggca atagttggcc ctgaccgaaa cggccaattt
    32761 tatttttgtc gttatttaca ccgcggtttc gcattcattg cctgatgcga cgtttacacc
    32821 cgtccgtagc gatccgcgaa gcgcaccaca tcatcctctt cgagataaga gccggagcgc
    32881 acttcaatta aatcgagcgg aattttcccc gggttttcca ggcagtgcgt cgcccccagc
    32941 ggaatataaa tggactcgtt ttcaccaagc agtttgatat caccatcaat ggtgactttt
    33001 gccgttcccg cgaccactac ccagtgttcc gcgcggtgat ggtgcatctg taccgacaaa
    33061 ccctcgcccg gtttcacggt gatgcgtttc acctggtagc ggtcgcccgc gtcgatagag
    33121 tcatatttgc cccacggacg atacacttcg cgatgcaccc gatgctcatg gcgaccatcg
    33181 gctttgatct gctcgaccac ttttttcaca tcctgtaccg cgttacggtc ggcaatcagc
    33241 accgcatctt tggtctgcac cactaccaga tctttcaccc cgacggtggt gactaggcct
    33301 gattcggcat acacatagct gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg
    33361 ttgccctcgg cggtgtgggc gctgatctcc cataatgaag accaggaacc aacatcgctc
    33421 cagcccgcat ccatcggcac cacaacagca tctgccgtac gttccatgac cgcgtaatcc
    33481 accgactctt ccgggcaggc gagaaacgct tcttcatcta cacgaataaa atcgagatcc
    33541 ggatcgacgg cgctcatcgc tttttcgcag gcatcgagga tatccgggcg atattttttc
    33601 agttcttcga gatagcgtcc ggcgcggaac aggaacatgc cgctgttcca gtaatattcg
    33661 ccgcttgcta cataggcctg ggcggtttcc agattcggtt tttcgacaaa ctgcgccact
    33721 tcaaaggcca ccgtatcctg ctcacccgca gacacttcac cgcgacgaat atagccataa
    33781 ccggtttctg gtagatccgg cacaatgcca aaggtcacca gcttgcccgc ttcggcataa
    33841 ggcatggcat tacgcacggc ggcacggaac gcgtctttat cggcaatcac atgatccgcc
    33901 gccaatacca gcattaacgg gtcgctctcc gggctatgac gttttgccgc cagcgccgcc
    33961 agtgcaatgg caggtgcagt gttacgccct gccggttcga gaataatgtt ctcggtgagt
    34021 ttgttcagtt gacgcagctg ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc
    34081 accgggcttt cgcactccac gccgttcagg cggcagatgg tggtttgcag catggtgaga
    34141 tcgcctttca ggcataaaaa ctgcttgggg taaagtacgc gggaaagcgg ccataaacgg
    34201 ctaccggagc cacctgccat cacaactgga tagagtttcg actgcgccat aattatcccc
    34261 gaatatcatt tataaattga cgtaacacgt tctctttatc gagcgtgcgt tcggcatatt
    34321 cacgtgccac cgtgttatgt ttgggcagca ggagcgcctg acgaatcccc gccaccagcg
    34381 cctcgaccga ttccggttct acgcaaaccg caatacccgg aaaggtttcg caaagctgcc
    34441 ccagttctgt gtgggcctct gcggtaatca ccgcgttacc gcctaccgcc agaatattgg
    34501 tcagtttcga cggcaatacg gcatctgccg cgccgcgttt ttacaccacc agatggcaat
    34561 cgcccatctt cagcagtgcg ggtaaagcgt catacgattg cagcgggaaa aattgcatgt
    34621 tgcgcagtcc acgctgctgc gccatttttt ccagccgcgc tttgccgccg ccctgcccga
    34681 caatggcaaa aagcagcggt tcatcgcgca ggagatcggc agcttcaata acgttttcca
    34741 gcccctgctt ttcaccaata ttgccggagt aaagaatgat ttttttgtta tccggcaggc
    34801 caagctggtt acgaagggca tcaacatcgg cgtctgcaac atgctgaaaa cgggcaattt
    34861 ccgaccagtt ggggaagaag atgacgttat ccgccgccac gcctttttcg atggctttat
    34921 tcatcatcga acgcgaaatc gtggaaacgt tatcgacgtt atgcagtccg ctacgttcga
    34981 acgccgttgc cagctgtgcc actttgccgc ctttgccttt tccggcaagg cccagcccca
    35041 gcatggcgtc cacttcgtaa tcctgaatat gcagcacggt acgcgcacca gagagcttcg
    35101 ccagcaggcg cattcccggc gtgcaaaaca gcgttggcac tacgccaata atgcgatccg
    35161 gcttccagcg acgttgcgcc atcagtggaa agaaactgct gacggcaaaa ctgccgagat
    35221 gcaacaagcg tttcagggtg ctcggctgtt ttggcacgta cagcgggcag cgccacaccg
    35281 tggcggcccc ttcttctcgt ttgtagcgcc aggcggaata gttctcaccc acctgccatt
    35341 gcgggtagta aggcggtgcg gtaatgaccc gcacctcatg accttgtgcc gccaaccatt
    35401 ccaccatctc gccggtgtat ttgccgatgc cggttaactc cggcgagtag ttaatgccgt
    35461 agaccagtat tttcataatc cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg
    35521 gctgttagcg tgaacatcgt tactggcgag cagcgcgtcc ggcgtcagcc agcggtaatc
    35581 gtcatgctgc tcatccggca gtaacagctc ttcttccgct actctgaagc gaaaaccgag
    35641 caccacatag tgagtggtga aatccgtgcc ggagaagtta tcgtcataaa aatgctgcca
    35701 gacaccgtaa aactggcctg ctgttatcgg cagacgcagc cccagttccg ccatcgtcag
    35761 ccgctcaaat gcggcttcca gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca
    35821 gtaaccctgc gccgggcggt tggttctttt gccaagcaga aactcgccgc gactgttctc
    35881 gacaataaag tcgagagaga caagcggtgt ggagcgcact acagtggcaa agtcttcctg
    35941 acgtaaaaac atcattcccc ccgaaagcgg tcttgattct caaggaacca ctggtaagtg
    36001 ctggcaagcc ccgcttccag tgagatttcg tgataccagc caagctgatg caggcgcgtc
    36061 acatccagca gtttgcgcgg cgtaccatcc ggtttgctgg catcaaaaac cacccgacct
    36121 ttgtaaccca ccactttggc gatggtttgc gccagctcgc ggatagtgca gtcaacgccc
    36181 gtgccgacgt taatgtgcga cagcatcggt tggctattct ccagccagac ttcatgcgcc
    36241 agctccatga catgaatgct cgccgccgcc atatcatcga cgtgcaggaa ttcacgcatc
    36301 ggtgtaccgc tgccccatac caccacgtcc ggcgcattct gtgccgtcgc ctcgtggaag
    36361 cgacgcagca atgctggtat cacatgggag ttactcgggt ggaagttgtc atgcggcccg
    36421 tacaggttgg tcggcatgac agagcggtaa tcgcgtccat actggcggtt gtaagattcg
    36481 cacagtttga tcccggcgat tttggcaatg gcgtaaggct cgttggtcgg ctccagcgtg
    36541 ccctgcaata actcgctttc tgccatcggc tgttttgcca gtttcgggta gatgcaggac
    36601 gatccgagaa acatcagttt gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc
    36661 tcaatcatca tgttctggta gatgaaatcc gccggatagg tgttgttggc aacaatgccg
    36721 cccactttcg ccgccgccag atagacctgg tcaatgcgtt cgctggcaaa gaaatcatgc
    36781 acggcgcggc tgtccagcag gttcagttca tcgcgggtac gtaataccag ctccacatca
    36841 ccgcgctgtt cgagctgccg cttgatggca gaaccgacca tcccacaatg gccagcgata
    36901 aaaatgcgtt gtttacttat gcttatgact ccagcgcgat cgccacgtcg tagccgtgag
    36961 atttcagcag agagtgtttt ttcgccgctt cgaggtcatt agccaccatt tcagacacca
    37021 tctctctgag ggtgatttcc ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgc
    37081 cgagcagcgt ttcaacttct gccggacgga agtaacgcgg atcaacggcg ataatcacat
    37141 cacccggttt aacgcccggc gcgtcatgcc cggtgacgga aaccacaatg cccttctctt
    37201 caacacccgt gccttcaaag cgcagtttga tgcccagctg tgctgccgcc atttccacaa
    37261 actgacgtac ggagtactga acgccggtcg cgataacgaa atcttccggc tgttcctgtt
    37321 gcagcatcat ccactgcatt tttacgtagt ctttggcgtg gccccagtca cgcagggaat
    37381 ccatatttcc gaggtacagg cacgactcca gcccctgggc gatgttggcg attgcgcggg
    37441 tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga ttcatggttg aagagaattc
    37501 cgttacaagc gtacatgccg taggattcgc ggtagttaac ggtgatccag taggcgtaca
    37561 gtttggcgac cgcatacgga gatcgcgggt agaacggcgt ggtttctttc tgcggaattt
    37621 cctgcaccag accatacagt tcagaggtgg aagcctgata gaaacgagtt ttcttttcca
    37681 gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc catcgcgtcg acgtcagcgg
    37741 tatattctgg tgactcaaaa gagaccgcaa cgtggctcat tgcgcccagg ttgtacactt
    37801 catccggctg tacttcacgc aaaatgcgcg tcaggttaga ggtatcactc aggtcgccat
    37861 aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct
    37921 cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac ctcgtaacct ttttccagca
    37981 gaaactctgc caggtaagaa ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg
    38041 acatgtctta ttcctctgta tttttgaatt tattcagttt caacgcgttc gcgtatcacc
    38101 actgcgggat tcccccggca aaccacattt gccggaagcg atttaaaaac actgcttcgt
    38161 gcacccacga cggtgccgtc gccgattgtg acgcctgggg caacaaagac atcggttgcc
    38221 agccagcatt tctcgccaat cacaataggc gtggcgttaa tggtgaaatg ttgacttgca
    38281 tggtcgtggc taccggtgca taaataactt ttttgcgata tcaccgaatg tgcgccaatg
    38341 gttatttcac cgagggtata taaattgacg tcatcgccaa cccacgcgta atcacctaag
    38401 gttaatttcc acggataggt aatttttact gacggacgaa taactacgtt ttttcctatt
    38461 tttgctccga ataaacgtaa taaaaaagcc cgccagcgat acaatacttg tggcgaccag
    38521 gcaaatattg ttgcctgtac tgcccaccat aattgcactt taatagcgtt gccgccccgg
    38581 aaccctttcg gcaccgagaa accgcttaaa tcttgcattg gttttcctta tattcagact
    38641 ttgttatata aggctttagt cttacctgtc gtacgttggc gtaaatgcca ggataattct
    38701 gcccagaagc caggtacatg taatatctgc cgttggactt ttttggcgtc agcac
//
LOCUS       DADWTH010000029        37864 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.029, whole genome shotgun sequence.
ACCESSION   DADWTH010000029 DADWTH010000000
VERSION     DADWTH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.029"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..2005
                     /gene="speF"
                     /locus_tag="JNY02_001577"
                     /pseudo
     CDS             1..2005
                     /gene="speF"
                     /locus_tag="JNY02_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="JNY02_001578"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="JNY02_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBB9433162.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGTSLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNY02_001579"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNY02_001579"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBB9433163.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNY02_001580"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNY02_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBB9433164.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="JNY02_001581"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="JNY02_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBB9433165.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6677..6970
                     /gene="ybfE"
                     /locus_tag="JNY02_001582"
     CDS             6677..6970
                     /gene="ybfE"
                     /locus_tag="JNY02_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBB9433166.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="JNY02_001583"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="JNY02_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBB9433167.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="JNY02_001584"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="JNY02_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBB9433168.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="JNY02_001585"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="JNY02_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBB9433169.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="JNY02_001586"
     CDS             complement(8459..8785)
                     /locus_tag="JNY02_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433170.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNY02_001587"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNY02_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBB9433171.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="JNY02_001588"
     CDS             complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="JNY02_001588"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBB9433172.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="JNY02_001589"
     CDS             complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="JNY02_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBB9433173.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14952..15752
                     /gene="nagB"
                     /locus_tag="JNY02_001590"
     CDS             14952..15752
                     /gene="nagB"
                     /locus_tag="JNY02_001590"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBB9433174.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15812..16960
                     /gene="nagA"
                     /locus_tag="JNY02_001591"
     CDS             15812..16960
                     /gene="nagA"
                     /locus_tag="JNY02_001591"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBB9433175.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAGAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPNFKITKTIVNGNEVVTQ"
     gene            16969..18189
                     /gene="nagC"
                     /locus_tag="JNY02_001592"
                     /gene_synonym="nagR"
     CDS             16969..18189
                     /gene="nagC"
                     /locus_tag="JNY02_001592"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBB9433176.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18237..18979
                     /gene="nagD"
                     /locus_tag="JNY02_001593"
                     /pseudo
     CDS             18237..18979
                     /gene="nagD"
                     /locus_tag="JNY02_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19376..21040
                     /gene="asnB"
                     /locus_tag="JNY02_001594"
     CDS             19376..21040
                     /gene="asnB"
                     /locus_tag="JNY02_001594"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBB9433177.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYARDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21420..21496
                     /locus_tag="JNY02_001595"
     tRNA            21420..21496
                     /locus_tag="JNY02_001595"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21454..21456,aa:Met,seq:cat)
     gene            21505..21589
                     /locus_tag="JNY02_001596"
     tRNA            21505..21589
                     /locus_tag="JNY02_001596"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21539..21541,aa:Leu,seq:tag)
     gene            21613..21687
                     /locus_tag="JNY02_001597"
     tRNA            21613..21687
                     /locus_tag="JNY02_001597"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21645..21647,aa:Gln,seq:ttg)
     gene            21722..21796
                     /locus_tag="JNY02_001598"
     tRNA            21722..21796
                     /locus_tag="JNY02_001598"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21754..21756,aa:Gln,seq:ttg)
     gene            21812..21888
                     /locus_tag="JNY02_001599"
     tRNA            21812..21888
                     /locus_tag="JNY02_001599"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21846..21848,aa:Met,seq:cat)
     gene            21937..22011
                     /locus_tag="JNY02_001600"
     tRNA            21937..22011
                     /locus_tag="JNY02_001600"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21969..21971,aa:Gln,seq:ctg)
     gene            complement(22165..23340)
                     /gene="ubiF"
                     /locus_tag="JNY02_001601"
     CDS             complement(22165..23340)
                     /gene="ubiF"
                     /locus_tag="JNY02_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBB9433178.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23486..24910
                     /gene="miaB"
                     /locus_tag="JNY02_001602"
     CDS             23486..24910
                     /gene="miaB"
                     /locus_tag="JNY02_001602"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBB9433179.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25063..26103
                     /locus_tag="JNY02_001603"
     CDS             25063..26103
                     /locus_tag="JNY02_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBB9433180.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26100..26567
                     /gene="ybeY"
                     /locus_tag="JNY02_001604"
     CDS             26100..26567
                     /gene="ybeY"
                     /locus_tag="JNY02_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBB9433181.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     MVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26657..27535
                     /gene="corC"
                     /locus_tag="JNY02_001605"
     CDS             26657..27535
                     /gene="corC"
                     /locus_tag="JNY02_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBB9433182.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27560..29098
                     /gene="lnt"
                     /locus_tag="JNY02_001606"
     CDS             27560..29098
                     /gene="lnt"
                     /locus_tag="JNY02_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBB9433183.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29495..30402
                     /gene="gltI"
                     /locus_tag="JNY02_001607"
                     /pseudo
     CDS             29495..30402
                     /gene="gltI"
                     /locus_tag="JNY02_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30572..31312
                     /gene="gltJ"
                     /locus_tag="JNY02_001608"
     CDS             30572..31312
                     /gene="gltJ"
                     /locus_tag="JNY02_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBB9433184.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTASRVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            31312..31986
                     /gene="gltK"
                     /locus_tag="JNY02_001609"
     CDS             31312..31986
                     /gene="gltK"
                     /locus_tag="JNY02_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBB9433185.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            31986..32711
                     /gene="gltL"
                     /locus_tag="JNY02_001610"
     CDS             31986..32711
                     /gene="gltL"
                     /locus_tag="JNY02_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBB9433186.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32829..33764
                     /gene="rihA"
                     /locus_tag="JNY02_001611"
     CDS             32829..33764
                     /gene="rihA"
                     /locus_tag="JNY02_001611"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBB9433187.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33848..35518
                     /gene="hscC"
                     /locus_tag="JNY02_001612"
     CDS             33848..35518
                     /gene="hscC"
                     /locus_tag="JNY02_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBB9433188.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASASDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35578..37029)
                     /gene="djlC"
                     /locus_tag="JNY02_001613"
     CDS             complement(35578..37029)
                     /gene="djlC"
                     /locus_tag="JNY02_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBB9433189.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIINRMLLAKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37026..37733)
                     /locus_tag="JNY02_001614"
     CDS             complement(37026..37733)
                     /locus_tag="JNY02_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBB9433190.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9256 a         9688 c         9587 g         9333 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatt agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggca cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatcg ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcttg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cttgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgactcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga actgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctgacagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    10321 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    10381 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    10441 cggttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    10501 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    10561 attaattcgc gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg gattgtttgc
    10621 tatttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaattcaa
    10681 tatattgcag agatcatgta ggcctgataa gcgaagcgca tcaggcaatt tagcgtttga
    10741 aatttattgt ctatttattg ccggatgcga cgtaaatgcc tcacccggct tggtagaact
    10801 taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc
    10861 ggtttttcgg ccgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg
    10921 aatgctttac ccgctaccgc atctttcagc gacggttcag caaagccctg tttgatcacc
    10981 agcgattccg ggttaatcac cgacaggaaa tcatccgcag cacctgggtt aggcacgctg
    11041 aacagacgat catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga
    11101 ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa
    11161 gtacagaaga tggtggtgat attaccttcg gcatctttct ccacgcgttc agccttaatg
    11221 acgtaagcat tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta
    11281 gcttcttcgc ggaaatcggc gcgatcgatc caaatttcac cgctaaacgg cacctgacgg
    11341 ctccccattt ccggtttgtt cggatggttc ggcatggtga ccatttcgcc ttcgccctga
    11401 tagttttcga taaccagttt caccggatcg ataactgcca ttgcgcgcgg cgcattttcg
    11461 ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg
    11521 gtcacgccga tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc
    11581 agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc
    11641 agcaggttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg
    11701 tactggcgcg gatgaacagg aatggtgatg ttatccagta cccagtcgta cagacgacgg
    11761 ttgtcctgga actcaagcgt acacagagag tgcgtaatac cttccagcgc atcgctgatg
    11821 caatgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca
    11881 gcaaatttaa tgcggtacag caccggatcg cgcattacga taaatggcga cgccatgtcg
    11941 attttcgcac gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac
    12001 agcgccaagt tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgcgtc
    12061 agggtaccgc ggtattcgcg gatctgttcc ggtgtcagtt catcaacgta cgccaggcct
    12121 ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg
    12181 acgttaccag accagtgaaa acctaaccac tctacgtcgt ttttgatcga atcgacatac
    12241 tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag
    12301 tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca
    12361 ttcggctccg gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca
    12421 tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc
    12481 tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg
    12541 ttagtttttt aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag
    12601 cggcagagaa gatctccacc gcttgtcatt gttggatgcg acgctgccgt gtcgcatcag
    12661 gcatacagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
    12721 ctgagcttta atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat
    12781 catcgagcgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
    12841 gcttacctgc gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
    12901 gtcgataccc atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg
    12961 gttggtgttg aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt
    13021 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
    13081 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
    13141 tggtacagcc tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc
    13201 agcggctacc ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt
    13261 cgcgccaaca atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag
    13321 acgtttacac atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt
    13381 aatacaggca tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt
    13441 tgccagttga gtcagacctt cttcagtatt gctgttggct tcttcagtaa cgatctcgtc
    13501 ttctttatct tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac
    13561 cacgaagtag atagcgaaga agataacgcc catcaccagc agcatccaga cgttctggct
    13621 ggcggccggc aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc
    13681 ggcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
    13741 gaggtacagc agcggcgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
    13801 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
    13861 cggtgctgcg aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa
    13921 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
    13981 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
    14041 cagcacctga tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga
    14101 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
    14161 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
    14221 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
    14281 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
    14341 ttctgggttg atgatcacca tcgctttggt taacacaaag taacctaccg cacccgccag
    14401 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
    14461 tgcgaggtta tcaaaaattg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
    14521 tggctgaccg aatcgcagca acagcgccgc caccggcagc accgcgatag ggagctgtaa
    14581 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
    14641 ctatttggct cgtttaaagc cgtattttta ttttgctgca aattttactg ccgatgttct
    14701 gtaatcagat tgttagatca tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa
    14761 ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
    14821 tggctaaacc agaaaactta ttttatcatt caaaaaatca agtcggattg acgcctgtct
    14881 gcgcaaatcc aggttacgct taaagatgtc taatccgcca acggcttaca ttttacttat
    14941 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
    15001 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccaac tgccgatcgt ccgtttgtac
    15061 tgggcctgcc gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata
    15121 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
    15181 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
    15241 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgatgccg
    15301 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
    15361 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
    15421 ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg
    15481 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg
    15541 aagaagtgat gattctggtg ctgggtagcc agaaagcact ggcgctgcag gccgccgttg
    15601 aaggttgcgt gaaccatatg tggaccatca gctgtctgca actgcatccg aaagcgatca
    15661 tggtgtgcga tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
    15721 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctgg ttccttgctc
    15781 agggcaatat tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt
    15841 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
    15901 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
    15961 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
    16021 caccgctgga gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
    16081 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
    16141 cgtgcgcgtt atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct
    16201 ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa
    16261 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
    16321 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
    16381 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg
    16441 gattaccttt gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg
    16501 cctggcgggc gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct
    16561 gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct
    16621 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
    16681 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
    16741 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
    16801 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt gacgttgaaa aacgtctcgg
    16861 cacgctcgcc gcaggtaaag tagccaacct gaccgcattc acacctaatt ttaaaatcac
    16921 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
    16981 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
    17041 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
    17101 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
    17161 gttgatcagc aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc
    17221 aatttccacg caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat
    17281 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
    17341 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaagctg
    17401 cgcgagctaa tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa
    17461 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actgaaagaa
    17521 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
    17581 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
    17641 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa
    17701 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
    17761 ctggaaacta tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag
    17821 ggctaccaga gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg
    17881 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggtaaa
    17941 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc tggtgaaatc
    18001 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac ccaagcgctg
    18061 aaggcgtttc gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc
    18121 ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
    18181 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
    18241 ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac
    18301 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
    18361 ccaactatcc ttcgcagact gggcaagatc tggcgaaccg ctttgccact gccggtgtcg
    18421 atgtgccgga tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc
    18481 aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag
    18541 ccggtttcac tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acacgttcct
    18601 acaactggga catgatgcat aaagcagcct atttcgtcgc taacggtgca cgctttatcg
    18661 ccaccaaccc ggacacggtt tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa
    18721 aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcggc
    18781 attaaacaaa atgcaggcgc attcggaaga aacagtgatt gtcggcgata acctgcgtac
    18841 cgatattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt ctggtgtttc
    18901 atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc
    18961 tgaaatcgac gttatctgaa taccccccac gtctgcggac gtggttggct ctgatgacaa
    19021 acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc aatatattga
    19081 attttcatgc ttttgtaggc cgtataaggc gttcacgccg catccggcat gaacaaatcg
    19141 cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc cgctaaaaat
    19201 tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat cgacaatcac
    19261 cattacgttt tttatttttg cactgccaaa tagcttgcgc acagtgctgc tttgcggcat
    19321 ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga aggttatgtg
    19381 ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta agaaagcact
    19441 cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt atgcccgcga
    19501 taacgccatt ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc
    19561 tctctacaac caacaaaaaa cccacgtact ggcggtaaac ggtgaaatct acaaccacca
    19621 ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg actgtgaagt
    19681 gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc agggcatgtt
    19741 tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg accatctggg
    19801 gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg cctcagaaat
    19861 gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga gctatttgtg
    19921 gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact ttgatgcagt
    19981 gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt ccgttaaaag
    20041 ccatctgatg tctgatgtgc cttacggtgt gctactttct ggtggtctgg attcctcaat
    20101 tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga
    20161 agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac cggatcttaa
    20221 agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc acttcactgt
    20281 acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt atgatgtgac
    20341 cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat
    20401 taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc tttacttcca
    20461 taaagcgccc aacgctaaag aactacatga agagacggtg cgtaaactgc tggccctgca
    20521 tatgtatgac tgcgcgcgcg ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt
    20581 tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccgc aggataaaat
    20641 gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgagt catacctgcc
    20701 cgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt acagttggat
    20761 cgacaccctg aaagaagtgg cggcgcagca ggtttctgat cagcaactgg aaactgcccg
    20821 cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc gcgagatctt
    20881 tgaagaactg ttcccacttc cgagtgccgc tgagtgcgtg ccgggcggtc cttccgtcgc
    20941 gtgttcttca gctaaagcga tcgaatggga tgaagcgttc aaaaaaatgg acgatccgtc
    21001 tggtcgtgcg gttggcgttc accagtcggc gtataagtaa gaaatgcggt actgccccgg
    21061 agaaatccgg ggctgtttta gttattgcgc tgcttgttga ttctgatatg cagtaaggaa
    21121 atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca gtatttcata
    21181 gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtacgac aaaagaatgg
    21241 ctgtagggtg gggaaaaaac acaaagtaaa caataattga cgaatatagc gccacgctgt
    21301 tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag gttgacgctg
    21361 caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga aaaaaagatg
    21421 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
    21481 cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat tggtagacgc accagattta
    21541 ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat tcaccagaaa
    21601 gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    21661 ctggttcgaa tccaggtacc ccagccatat tcttcgagta agcggttcac cgcccggtta
    21721 ttggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat
    21781 ccaggtaccc cagccatcga agaaataatc tggctacgta gctcagttgg ttagagcaca
    21841 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa ttctgaatgt
    21901 gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc
    21961 accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc acattaaaaa
    22021 agctcgcttc ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat gcgatgttga
    22081 cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac ctacggttgg
    22141 cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg cctgacgttt
    22201 caacacgcca gcacgctctg ccgccattaa cccgagatta cgcacaaaac gcagcggcgg
    22261 cagattatta ctgaatccgg cataaaacag atccatacca ctttgcataa tgaagttatc
    22321 cgccatgcgc cgcatctggt aacgcttgag gacaggataa ctggcccacg cttcaccgta
    22381 gctgcgggca ttcaccagaa catcaatcag ggcatcgaca tcacgataac caagattcac
    22441 cccctgcccc gccagcggat ggatggtatg cgcggcatcg cccaccagcg caagccccgg
    22501 ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac gcaccagcgg caagcggtgt
    22561 aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc gcctggagtt gcgccatatt
    22621 catattctgc aactggcgaa tacgcgccgg agagtcatac cacaccagcg atgcccagtt
    22681 atcaaacaac ggcagaaacg cacgcggtcc gtccggggta aattgctgcc aggtgctgtc
    22741 gccgggatcg ttctcacact gtacgctaat caacatgcac gactgcgcat actgccaggc
    22801 atgaacgcca attcccgcca tctgccgcac ctgagaattt gcgccgtcgg caccaatcac
    22861 cagtttcgcg cggatcgttt caccgccctt cagttccagc tcctgaagat cattatctcg
    22921 atgtaatgca atcagcgagg ttggcacacg taacgttact ttcggatgcg cttccagcgc
    22981 ctgccacaac gcctgttgca ggactgtgtt ttccaccatg tagccaagca acggcaactt
    23041 taattcggct gcgtcaaata ccacatgtgc cgtttcccac tcccaggttt ccagtctgcg
    23101 gtaaggatgg caacgcatag cctgtactgc atcccagacc cctacccctt taagcaatga
    23161 taccgaagcc gcgctgatcg ccgagatccg cacgtccggt tggctgtcag cgacaaacgg
    23221 tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg tgctgtgcca gccccagcgc
    23281 cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt gatttgtcat
    23341 ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc ccaagggctg
    23401 ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc tgcattcctg
    23461 gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa cctggggctg
    23521 tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcca cccacggcta
    23581 tcaactgacc gacgtggcgg aagaagcgga tgtgctactg ctgaacacct gctcaatccg
    23641 cgagaaggct caggaaaaag tcttccatca gttgggtcgc tggaaactgt taaaagagaa
    23701 gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat
    23761 tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc accgtctgcc
    23821 ggagatgatc aactccgtgc gcggcgaccg cagcccggtt gtagatatca gcttcccgga
    23881 aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc
    23941 catcatggaa ggctgcaata aatattgcac ctactgcgtg gtgccttaca cccgtggtga
    24001 agaggtaagc cgtccgtctg acgatattct gtttgagatt gcccagcttg cggctcaggg
    24061 cgtgcgtgaa gtcaacctgc tcggtcagaa tgtgaatgcc tggcgtggtg agaactacga
    24121 cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga
    24181 tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca tcgaagtgta
    24241 ccgcgacacg cctgagctgg tgagcttcct gcatctgccg gtacagagcg gttccgatcg
    24301 cattctgaac ctgatgggac gtacccatac ggcgctggag tacaaagcga tcatccgtaa
    24361 actgcgtgcg gcgcgtccgg atattcagat cagttctgac ttcattgttg gcttccctgg
    24421 cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca atttcgacat
    24481 gagctacagc tttatcttct ctgcacgtcc aggaacgcct gccgccgata tggttgatga
    24541 tgttccggaa gaagagaaga agcagcgtct gtatattctg caagaacgta tcaatcagca
    24601 agcgatggca tggagccgtc ggatgctcgg caccacccag cgtattctgg tagaagggac
    24661 ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa aataaccgcg tggtcaactt
    24721 cgagggcaca ccggatatga tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc
    24781 gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa atgggtctgc gcatggcaga
    24841 aacaccggag tcggtgattg cccgcacccg caaagaaaac gaccttggcg tgggttatta
    24901 tcagccgtaa ttctcaggcc tgccgttccg gtgggccttg ttttttctct ctttatcccc
    24961 atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct tataggccgg
    25021 tgaatgttca gcttccggcg gcatacagat aagaggaacg gtttgaacat agacactcgc
    25081 gaaatcaccc tggagccagc agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat
    25141 gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg cgataaccac
    25201 tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct gcgtagcctg
    25261 tatgtcgata ctgccccgat gcgcggtcag attcaggata tcgaaccgga acagatccac
    25321 cttgcgatta aagaagcgcg ggtactggag caaagcgcgg agagcgtgcc ggagtacggc
    25381 aaagcggtca atatcaaaac taaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg
    25441 cagtacatcg ccaatattct cgaccatgac attaccttcg gcgttggccc ggcgggtacg
    25501 ggtaaaacct acctggcagt ggctgcggca gttgatgccc tggagcgtca ggaaattcgc
    25561 cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt cctgcctggc
    25621 gatttaagcc agaaagtaga cccgtatctg cgcccactgt acgacgcgct gtttgaaatg
    25681 ctgggctttg agaaagtcga gaaactgatt gagcgcaacg ttattgaagt cgcgccgctg
    25741 gcctatatgc gtggtcgtac gctgaacgac gcgtttatca ttctcgatga gagccagaac
    25801 actaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc aaaagcggtt
    25861 atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg cttacgtcat
    25921 gccattgaag tgctggccga tgtcgaagag atcagcttta acttcttcca cagcgaagac
    25981 gtggttcgtc acccggtggt ggcgcgtatc gttaacgctt atgaagccag ggaagaagca
    26041 gaacaaaaac gtaaagcggc gctggcagca gaacgcaagc gcgaagaaca ggaacaaaaa
    26101 tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taattccggg ttaccggaag
    26161 agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa gagtcggaag
    26221 tgactattcg catggtcgat accgccgaaa gccacagtct gaatctgacc taccgcggta
    26281 aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc atggagatgt
    26341 cgctactggg cgatctggtt atctgccgtc aggtggttga gaaggaagct caggagcaag
    26401 gcaaaccact ggaggcgcac tgggcgcata tggtggtgca cggcagtctg catttgttag
    26461 gttacgatca cattgaagat gacgaagcag aagaaatgga agccctcgaa acagagatta
    26521 tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt gccaggcatt
    26581 tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta actaacatga
    26641 gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga taagcaacaa
    26701 gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga aaaaccgtga
    26761 cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg aagatacgcg
    26821 cgatatgctc gaaggggtga tggacatcgc agaccaacgc gttcgcgaca tcatgatccc
    26881 ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat
    26941 catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc acattgaagg
    27001 gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag ccttcggcat
    27061 ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag accggatgct
    27121 gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat tcggtggggt
    27181 ttccggtctg gtgaccattg aagacatcct ggaactgatt gttggtgaga ttgaagacga
    27241 gtatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg
    27301 cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta gcgatgaaga
    27361 agtcgacact atcggtggtc tggtgatgca ggcgtttggg catcttccgg cgcgtggcga
    27421 aactatcgac atcgacggtt accagttcaa agtggcgatg gccgatagtc ggcgtattat
    27481 tcaggttcat gtcaaaatcc cggatgactc accccagcca aagctggatg aataaaaccg
    27541 aaactggata gataactaca tggcttttgc ctcattaatt gaacgccagc gcattcgcct
    27601 gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg
    27661 gcctgcggcg attatttcgc tgatggggct tcaggcgttg acctttaacc gtcgcccact
    27721 ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg gtattaactg
    27781 ggtctatgtc agcatcgcga cctttggcgg gatgcctggc ccggttaaca tcttcctggt
    27841 ggtgctgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg tgctgtcgcg
    27901 tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc gcccctgctc tctggcaagt
    27961 gaccgagttt ctgcgcggtt gggtgctgac tggcttcccg tggttacagt tcggctatag
    28021 ccagattgat ggcccgttaa aagggctggc accgctaatg ggcgtggaag ccattaactt
    28081 cctgctgatg atggttagtg gcctgctggc actggcgttg gtcaaacgca actggcgtcc
    28141 gctggtggtg gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca tccaatggtt
    28201 taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata ttccgcaatc
    28261 gctgaaatgg gacgaaggcc agcttcttaa tacgctaaag atttactaca acgcaacggc
    28321 accgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa ccgatctgga
    28381 aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgtgata aaggtagctc
    28441 gctggtaacc gggattgtcg acgcgcgtct caataagcag aaccgctacg atacctacaa
    28501 caccatcatc acgctgggta aaggtgcacc gtacagctac gaatcagccg atcgctataa
    28561 caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt
    28621 agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata tccagccgcc
    28681 gctgtcggca aatggtattg agcttactgc ggctatttgc tacgagatca ttctcggcga
    28741 gcaagtgcgc gataacttcc gcccggatac cgactacctg ctgactatct ccaacgatgc
    28801 gtggtttggt aaatctattg gtccatggca acacttccag atggcgcgaa tgcgtgcgct
    28861 ggagctggcg cgcccactgt tgcgcagcac caacaacggc attacggcgg tgattggccc
    28921 gcagggtgag attcaggcga tgatcccgca gttcacccgc gaggtgttaa ccactaacgt
    28981 gacgccgacc accggactca caccatacgc acgtaccggc aactggccgc tgtgggtgct
    29041 gacggcactg tttggttttg ctgctgtgtt gatgagtctg cgtcagcgac gtaaataatc
    29101 ccttcttatc gtgccggaat acatcccata tttcggcacg ctcaccggat gcggcatcag
    29161 tgcattatcc ggataacaat attctcctat ccaactttgg cacatctatt gctttgttat
    29221 acaaggcaaa gcttgaacca gcatcagtac aacccggttg caccacagca atccaccggt
    29281 agcaccaaaa gagtgcattt taagaatttt gcttcagaat ggtgcggtgc acctcgcaaa
    29341 aacaaacaat aaaacatcaa tatctttaca tttagccaaa ctaaatgtta cctaacaggc
    29401 acaacactgc acaataaagt tgcagacgat aacaacacaa acactcacaa cgggtatcca
    29461 tgcgttctta acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac ctgccacagc
    29521 aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc
    29581 aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt gtcgtcggtc accgtgaatc
    29641 ttcagtacct ttctcttatt acgacaatca gcaaaaagtg gtgggttact cgcaggatta
    29701 ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac aaaccggact tgcaggtaaa
    29761 actgattccg attacctcac aaaccgtatt ccactgctgc aaaacggcac tttcgatttt
    29821 gaatgtggtt ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact
    29881 attttcgtgg tcggtacgcg cctgttgacc aaaaagggcg gcgatatcaa agattttgcc
    29941 gacctgaaag gcaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac
    30001 aaactgaatg aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac
    30061 tctttccgca ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgctctgctg
    30121 gccggtgaac gtgcgaaagc gaagaaacca gacaactggg atatcgtcgg caagccgcag
    30181 tctcaggaag cctacggttg catgttgcgt aaagacgatc cgcagttcaa aaagctgatg
    30241 gatgacacta tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataaatgg
    30301 ttcaaaaatc caattccgcc gaaaaacctg aacatgaatt tcgaactgtc agacgaaatg
    30361 aaagcactgt tcaaagaacc gaatgacaag gcactgaact aattacaaga accaggggcg
    30421 gaaattccag ccctctcgat tgttacgtag cacggacaga ctatacgcct gatggtcgtt
    30481 ccccatcggg cctgaaaacc gcaatacgct gggtaacaat cttcgagggt agcagttaac
    30541 gctgctaccc tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt
    30601 tttacaacaa gccccgttcg gcaataccac ctatcttggt tgggtctgga gtggttttca
    30661 ggtgacgatc gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt
    30721 cggcatttta cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga
    30781 actgttccgc aacgtgccgt tgattgtgca attctttacc tggtatctgg tgatcccgga
    30841 gctgctgccg gagaaaatcg gcatgtggtt taaggccgag ctggatccca atattcagtt
    30901 tttcctttca tccatgctct gcctggggct gtttaccgcc tcccgtgttt gcgaacaggt
    30961 acgcgccgct attcagtcgc taccgcgtgg gcagaagaat gccgcgctgg cgatgggcct
    31021 gacgctaccg cagacttatc gatacgtact gctgcctaat gcttatcgcg ttatcgtccc
    31081 gccgatgact tccgagatga tgaacctggt gaaaaactcc gccatcgcct cgaccatcgg
    31141 tctggtggat atggcggcgc aggcgggtaa attgctcgac tactcggctc acgcctggga
    31201 gtcgtttacc gccatcacgc ttacgtatgt gttgattaac gcttttatca tgctggtgat
    31261 gacgctggtt gaacgtaaag ttcgcctgcc cggcaacatg gggggcaaat aatgtacgag
    31321 tttgactgga gttccattgt tccttccctg ccatatctgc tcgacgggct ggtgatcacc
    31381 ctgaaaatca ccgtcacggc agtcgtgatt ggtattttgt ggggtaccat gctggcggtg
    31441 atgcgcttgt ccagcttcgc gcctgtttcg tggtttgcca aagcctacgt taacgtattc
    31501 cgctcgattc ctttagtcat ggttttgctg tggttttact tgattgtgcc gggatttctg
    31561 caaaatgtgc tgggattatc gccaaaaaat gatattcgtt taatttcggc gatggtggcg
    31621 ttttcgatgt ttgaagcggc ctactattca gagattatcc gtgccggtat tcaaagtatt
    31681 tcccgtggtc agtcgagcgc cgcgctggcg ttggggatga ctcactggca gtcgatgaaa
    31741 ctgattattc tgccgcaggc tttccgcgcg atggtgccgc tgctgctcac tcagggcatc
    31801 gtactgttcc aggatacctc gctggtgtat gtgttatctc tggccgattt cttccgtacc
    31861 gcctcaacca ttggtgagcg tgatggtacg caggtcgaga tgatcctgtt tgccggattt
    31921 gtttatttcg ttattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca
    31981 gcataatgat taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg
    32041 actgctcaac cgaagtgaaa aaaggcgaag tggtggtggt ttgcggcccg tctggttccg
    32101 gcaaatcaac gctgattaaa accgtcaacg gcctcgaacc agtgcagcaa ggtgaaatca
    32161 ccgtcgatgg tatcgtggtt aacgacaaga aaaccgatct ggcaaagctg cgctcccgcg
    32221 tcgggatggt attccagcat ttcgagctgt tccctcatct gtcgattatc gaaaacctga
    32281 ccctggcgca ggtgaaagtg cttaaacgcg ataaagcgcc cgctcgtgaa aaagcattga
    32341 aattgctgga gcgcgtggga ctttccgcac atgccaataa atttccggcg cagctttccg
    32401 gcggtcagca gcagcgtgtg gcaatcgctc gcgcattgtg tatggctcct attgcgatgc
    32461 tgttcgacga accgacatcg gcgctggatc cggagatgat caacgaagtg ctggacgtga
    32521 tggtggaact ggcgaacgaa ggaatgacca tgatggtggt gacccacgaa atgggctttg
    32581 cccgcaaagt ggcgaatcgg gtgatcttta tggacgaggg taaaattgtc gaagactcgc
    32641 cgaaagacgc tttcttcgat gatccgaaat cggaccgcgc aaaagacttc ctcgcgaaaa
    32701 tcctgcatta atcactctgg cgcgcatacc caattgcgcg ccatctcacg ctttatcgtt
    32761 tcgcccctcg tatctgttac atcctggcgt tacccttgtc gcaaagaagc acacaacaag
    32821 gagcaacaat ggcactgcca attctgttag attgcgaccc aggtcatgat gacgctatcg
    32881 caatagttct cgccctcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg
    32941 gaaaccagac accagaaaaa accttacgca atgttctgcg tatgctgacc ttgcttaatc
    33001 gcaccgatat tccggtggca ggcggcgcgg taaaaccgtt aatgcgtgag ttgattatcg
    33061 cggacaatgt gcacggcgaa agcggtctcg acggcccggc attaccggaa ccgacgttcg
    33121 caccgcaaaa ctgtacggcg gtggagctga tggcgaaaac gctgcgcgaa agtgcggaac
    33181 ctgtcacgat tgtttctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccatc
    33241 cggaactgca tagcaaaatt gcccgtatcg tgattatggg cggcgcaatg gggcttggta
    33301 actggacgcc tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct
    33361 tccagtcagg gatcccggtg gtgatggccg gtctggatgt tactcataaa gcgcaaatcc
    33421 acgttgaaga caccgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg
    33481 aactgctgga tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac
    33541 cgctgcatga cccatgcacc atcgcctggc tgttgaaacc cgagttgttt accactgttg
    33601 agcgctgggt tggcgtggaa acacagggga aatataccct gggtatgacg gttgttgatt
    33661 attattatct gactggcaat aaaccgaatg ccactgtaat tgtcgatgtt gatcgtcagg
    33721 gctttgttga tttactggct gatcgtctga aattttacgc ttaacactcg tcattgatta
    33781 agatcaccac gcgttacctg ggtggtctta atctatttat ctctttttct ttcaagatat
    33841 aaatgcaatg gataatgcag aactcgccat aggtatcgat ctcggcacta ccaatagttt
    33901 aattgccgtc tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt
    33961 aacaccatcc ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt
    34021 atcacggcgt acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag
    34081 taataccaac tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt
    34141 attacgctca ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt
    34201 gatctccgtt ccggcttatt tcagcgataa acaacgcaag catacccgtt tagcagcgga
    34261 cttagccggg ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcgta
    34321 tggcctgcat acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt
    34381 tgacgttacg gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctagcga
    34441 caacttcctc ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc
    34501 ggatgtcaca aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga
    34561 agcggcaaaa tgtagcaatc aatcgccaat gcacattcgc tggcagtacc aggatgaaac
    34621 gcgggaatgc gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg
    34681 cttgcgagtg ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga
    34741 cagtctggtg ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg
    34801 tctgtttggc aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc
    34861 agcaatccag gccgcctgcc gcttacgcag tgaagatatt gaagaagtga tcctcactga
    34921 tatttgccct tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc
    34981 gccgattatt gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat
    35041 gcacccggaa caggattcaa ttacggttaa cgtctatcag ggagaaaacc acaaagttaa
    35101 aaacaacatt ctggtggaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc
    35161 gattgatatt cgctttagtt atgatatcaa cgggttgctt gaagttgacg tgcttctgga
    35221 agacggcagc gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca
    35281 gattgaagag agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat
    35341 caatcgcacc tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg
    35401 agaagagatt ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc
    35461 ccgcgtcgat gaggtcaggc ggcgggcgag cgtttattta gccattgaga cctcataatc
    35521 acggctgccg gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca
    35581 actaaacaac atcgcgattt tgccgagagc ataacctata accagcaccg ttaaactgat
    35641 tttgataatg ctggtaatgc tctgtggttt ttcaccctca tcattttttt cttcatggtt
    35701 ttcaggtgtt ggctcatcga catcttcatc ctccggctcg agttttactt tcgccaacag
    35761 catccggttg ataatggcct ctaatggatg agcatctccg ccttccagcc ttgtattaag
    35821 acgggccagc atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata
    35881 caaatccagt aacggtgaat cttcttctgt ctcgctaccg actctcgatt tccaccacgc
    35941 ccatgctttg cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt
    36001 tccgctatgt aaaatttccg cccataacag ccgcgtccgg gcgctctcac caggaacata
    36061 tttcagcggt tgaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa
    36121 cgcgaaataa tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt
    36181 agatgggtaa ctttcccagt acgcgccgag atcagcaagc aggctttccc cctcgccgca
    36241 gtacaaacgt tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg
    36301 ccactgtttg gctacaggaa tgagttccac catacctgtt tgcaccgagc tgtaccagcg
    36361 caacagtttg cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa
    36421 aaacgcagtg acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc
    36481 aacagtctgg ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc
    36541 accagcctga atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc
    36601 attaaacgaa tgccagttca aacgctctgc caacaaagaa gcacaattta atgacagata
    36661 tcgcgcttct atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt
    36721 ccaggtattt aattgctgaa taaatttctg ccaagcggag ggctgaaaac gatcactttc
    36781 tgaatccagt aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc
    36841 ttcatcatct gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg
    36901 gcgtaatgct ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag
    36961 ataagcctgg cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca
    37021 tgttttcata ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat
    37081 ggctgccagg gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc
    37141 cgactcccgg cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct
    37201 tcgcactgag aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc
    37261 cagctgtgat agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct
    37321 tcttccgtca gccagttgtt gagcacaccg gcccgtgaca gaaaccccat tcgaacataa
    37381 tcccaggctt tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta
    37441 taaatcaacc ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag
    37501 cgttgccatg tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga
    37561 taaatagtct gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat
    37621 tcttttatcc cggtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt
    37681 atttccatgg gcgcagaaag cgcaaaaaga agatattgtt cttccttatt cattttttaa
    37741 taatccctta agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc
    37801 tttcaaaata aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt
    37861 cgca
//
LOCUS       DADWTH010000030        37625 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.030, whole genome shotgun sequence.
ACCESSION   DADWTH010000030 DADWTH010000000
VERSION     DADWTH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.030"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..174)
                     /locus_tag="JNY02_001615"
     CDS             complement(<1..174)
                     /locus_tag="JNY02_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBB9433191.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQYR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNY02_001616"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNY02_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBB9433192.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNY02_001617"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNY02_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBB9433193.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNY02_001618"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNY02_001618"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBB9433194.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNY02_001619"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNY02_001619"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBB9433195.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNAVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNY02_001620"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNY02_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBB9433196.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="JNY02_001621"
     CDS             complement(5178..5420)
                     /locus_tag="JNY02_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBB9433197.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNY02_001622"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNY02_001622"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBB9433198.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNTIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNY02_001623"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNY02_001623"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBB9433199.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="JNY02_001624"
     CDS             complement(8337..9311)
                     /locus_tag="JNY02_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB9433200.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="JNY02_001625"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="JNY02_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBB9433201.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVIEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /gene="mreB"
                     /locus_tag="JNY02_001626"
     CDS             11708..12751
                     /gene="mreB"
                     /locus_tag="JNY02_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBB9433202.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="JNY02_001627"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="JNY02_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBB9433203.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="JNY02_001628"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="JNY02_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBB9433204.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="JNY02_001629"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="JNY02_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBB9433205.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="JNY02_001630"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="JNY02_001630"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBB9433206.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /gene="yhdP"
                     /locus_tag="JNY02_001631"
     CDS             16537..20337
                     /gene="yhdP"
                     /locus_tag="JNY02_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBB9433207.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="JNY02_001632"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="JNY02_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBB9433208.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNY02_001633"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNY02_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBB9433209.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="JNY02_001634"
     CDS             23178..23381
                     /locus_tag="JNY02_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBB9433210.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="JNY02_001635"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="JNY02_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBB9433211.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="JNY02_001636"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="JNY02_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBB9433212.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGMLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="JNY02_001637"
     CDS             26386..26658
                     /locus_tag="JNY02_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBB9433213.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JNY02_001638"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JNY02_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBB9433214.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JNY02_001639"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JNY02_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBB9433215.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="JNY02_001640"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="JNY02_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBB9433216.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JNY02_001641"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JNY02_001641"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBB9433217.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="JNY02_001642"
     CDS             complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="JNY02_001642"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBB9433218.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31926..32324)
                     /locus_tag="JNY02_001643"
     CDS             complement(31926..32324)
                     /locus_tag="JNY02_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBB9433219.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32518..33645
                     /gene="zapE"
                     /locus_tag="JNY02_001644"
     CDS             32518..33645
                     /gene="zapE"
                     /locus_tag="JNY02_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBB9433220.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33864..34292
                     /gene="rplM"
                     /locus_tag="JNY02_001645"
     CDS             33864..34292
                     /gene="rplM"
                     /locus_tag="JNY02_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBB9433221.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34308..34700
                     /gene="rpsI"
                     /locus_tag="JNY02_001646"
     CDS             34308..34700
                     /gene="rpsI"
                     /locus_tag="JNY02_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBB9433222.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35095..35733
                     /gene="sspA"
                     /locus_tag="JNY02_001647"
     CDS             35095..35733
                     /gene="sspA"
                     /locus_tag="JNY02_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBB9433223.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35739..36236
                     /gene="sspB"
                     /locus_tag="JNY02_001648"
     CDS             35739..36236
                     /gene="sspB"
                     /locus_tag="JNY02_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBB9433224.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36279..37625)
                     /gene="dcuC"
                     /locus_tag="JNY02_001649"
                     /pseudo
     CDS             complement(36279..37625)
                     /gene="dcuC"
                     /locus_tag="JNY02_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
BASE COUNT         9109 a         9412 c         9904 g         9200 t
ORIGIN      
        1 tctgtactga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgcggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaga ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac ggcattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac gagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagtgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga cgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accctggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 agcactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctggcgtagt ggtcaggata ccgagcaggt aatagaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgtg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gagccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcaccgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg acatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttggcggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cctaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttggtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg atgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccttt
    26461 ggtctggcta aagataaggt acgcgatctc gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaaa gcccccgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtacacac taattattga
    27061 actaattaat accacgctaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcgaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggcgtg
    30661 atcatttcag cgctggccga cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt
    30721 ggtttgccgt tacgcagcag gccaagcttc actttcgtgc ccggctctgt agtcgcgata
    30781 cgagagcgca actcagcaaa gctattcagc ggtttgccgt tgaggctggt aataatatcg
    30841 cccgctttga cgcccgcttt cgctgagcca gaacttggca acacttcgct gacaaacgcg
    30901 ccacgctgca cgtcaaggtt gaaggctttg gcgatatcgg cactcatctc ggtgcctttg
    30961 atgcctaaca aaccgcgttt gatttcacca aagtcgataa gctgctgcgc cagtgttcgc
    31021 gccatattac tggggatggc aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt
    31081 gcagtgttga tgccaattaa ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg
    31141 cggttaatgg aagcatctgt ctggataaag ttttccagac cttcaagatt caacccgctg
    31201 cggcctaatg cggaaacaat gccagaggtg gcggtttgcc caaggccaaa tgggttaccg
    31261 accgctacgg caaaatcacc gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt
    31321 aatttgctcg ggttttgaat ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc
    31381 agttttgcat caaactcgcg cccatcattg agctgaatac tgattttctg tgcctgatta
    31441 atcacatggt tgttggtcag cacatagcct ttactggcgt tgatgatgac accggaacct
    31501 aaaccttcga agggttgtgc aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat
    31561 tcttccggga ttttctgtcc ctgactggcc gttccttcca cccgtacgct caccactgcc
    31621 ggaagcactt tttccagcat tggagccaga ctggggagag gggcctgatc ggcaacctgg
    31681 cctggaatcg acgcgacggc ctgaaatgac gccgagagag ttaacccggc acttaacgct
    31741 aatgcactca acagctgggt ttgttttttc attattcctg ctctcgtacc tgaatgataa
    31801 gaaaagagat tcaaaacagt ttgatgttat tgaattttca gacggctaac aatgagacac
    31861 cagattaaat gatagtcggg gagggagaga agaggagggc gcaatggctg cgcccgaaaa
    31921 ataaattagt cgcgcttcgc gccagtacgc agcaggccgg atgcgccttc agaatagtcg
    31981 cgaggcatct gcaccggtgc ctgatcgttg ctggcttcag actctgccag acgattacgg
    32041 aacgggtttg cttcagcaga cagttccggc agcaggctgc tggagctttt tgccatgtgc
    32101 tgatacagct ggcgatagtc gtgcgccatg gtatccagta attccgcgct gcgggcaaag
    32161 tggctaacca gctcttcgcg atactcgtcc agttcagctt tattcttttc cagttcgtac
    32221 tgcaacgcct gttgctggcg tagtttacga ttaccaaaac gcatggccac agcaccaata
    32281 atgatgccga cgactaaccc aattagcgca tattcccagg tcatgaacat ctcccgttgt
    32341 cttttgtttc cgtagggtgt tggcttcagg ctccatgcct gcggctgatt atgccactat
    32401 aaccgttaat tccacagaag tggaatcccg actgcatatc gcgtagtgta gaacggcctt
    32461 tttttcgtca accgtgaaca acggcgcacc gattattcaa ggaataacaa taagatcatg
    32521 caaagcgtta ccccaacatc gcaatacctg aaggcgctca atgaaggcag ccatcaaccc
    32581 gacgacgttc aaaaagaggc cgtcagccgc ctggaaatta tttatcaaga actcatcaat
    32641 agcaggccac cagcccccag gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt
    32701 aaacgcgaag acacaaagca tacgccagtg cgtggcttat atatgtgggg cggtgtagga
    32761 cgcgggaaaa cctggctgat ggaccttttc tatcaaagcc tgccgggaga gcggaaacag
    32821 cgcctgcact ttcaccgttt tatgctgcgg gtgcacgaag agctaactgc cttacagggg
    32881 cagaccgatc cgctggaaat tattgccgat cgctttaaag ccgaaactga cgtgctctgt
    32941 tttgacgaat tttttgtttc tgatattacc gacgccatgc tacttggcgg tctgatgaaa
    33001 gccctgttca cccgcggtat taccctggta gcgacgtcaa atattccgcc ggacgaactt
    33061 tatcgaaatg gcctgcaacg tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat
    33121 tgtgatgtaa tgaacgtgga cgctggtgtt gattatcgac tgcgtacact cactcaggcg
    33181 catctgtggc tttcgccact caacgatgaa acccgggcgc agatggataa actatggttg
    33241 gcgctggcgg gggtgaaacg agaaaattca ccgacgttag aaatcaacca tcggccattg
    33301 gcgacaatgg gcgtcgagaa ccagacgctg gcggtttctt ttactacgct gtgcgtcgac
    33361 gcccgcagtc agcatgacta tattgcgctc tcacgcctgt ttcacacagt aatgttgttt
    33421 gatgtgccag tcatgacgcg gttgatggag agcgaagcgc ggcgctttat tgcgctggtg
    33481 gatgagtttt acgagcgcca tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa
    33541 atttatcagg gtgagcggct gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag
    33601 atgcaaagcg aagagtatct gaagcgcgag catttggcgg gttaaaacct gccacaaatc
    33661 acaaaaaggg gtcgatcttt gaccccgact tctctataat cctgcgaccc cacgttacaa
    33721 gaaagttttt ttcccaaaac tttttgtgtg ctggcatagg ctattcgaag gggtaggttt
    33781 gccggacttt gtcgtgtgaa cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta
    33841 actatttatt gggtaagctt ttaatgaaaa cttttacagc taaaccagaa accgtaaaac
    33901 gcgactggta tgttgttgac gcgaccggta aaactctggg ccgtctggct actgaactgg
    33961 ctcgtcgcct gcgtggtaag cacaaagcgg aatatactcc gcacgtagat accggtgatt
    34021 acatcatcgt tctgaacgct gacaaagttg ctgtaaccgg caacaagcgt actgacaaag
    34081 tgtactatca ccacaccggc cacatcggtg gtatcaaaca agcgaccttt gaagagatga
    34141 ttgctcgccg tcctgagcgt gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc
    34201 cgctgggtcg tgctatgttc cgtaaactga aagtttacgc gggtaacgag cacaaccacg
    34261 cggcacagca accgcaagtt cttgacatct aatcgggatt ataggcaatg gctgaaaatc
    34321 aatactacgg cactggtcgc cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca
    34381 acggtaaaat cgtaatcaac caacgttctc tggaacagta cttcggtcgt gaaactgccc
    34441 gcatggtagt tcgtcagccg ctggaactgg tcgacatggt tgagaaactg gacctgtaca
    34501 tcaccgttaa aggtggtggt atctctggtc aggctggtgc gatccgtcac ggtatcaccc
    34561 gcgctctgat ggaatacgac gagtccctgc gttctgaact gcgtaaagct ggcttcgtta
    34621 ctcgtgacgc tcgtcaggtt gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc
    34681 cgcagttctc caaacgttaa ttggcttctg ctccggcaga aaacaatttg cgaaaaaacc
    34741 cgcttcggcg ggttttttta tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct
    34801 ttttgctgcc gtctgaaaga caggtaaaac aaaaaccaca tcgccaataa gggtctaagt
    34861 caactatttc agactaaagc gcatctcttt ttccccattt ccggcatcca ctcaccacaa
    34921 aggtcgcaaa atctggtaaa ctatcatcca attttctgcc caaatgtcgg gtattgctca
    34981 ttttttgttt gattttcgaa caaagagagt cgttccttat tgggtaacac aacttctgac
    35041 tggccacctg gtggctggta gcagtaaaaa ttctgactat acctggaggt tttcatggct
    35101 gtcgctgcca acaaacgttc ggtaatgacg ctgttttccg gtcctactga catctatagc
    35161 catcaggtcc gcattgtgct ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa
    35221 aaggacaatc cgcctcagga tctgattgac ctcaacccga atcagagcgt tccgaccctg
    35281 gtggatcgtg agctgaccct gtgggaatct cgcatcatta tggaatatct ggatgagcgt
    35341 ttcccgcatc cgccactgat gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac
    35401 atgcatcgca tcgaaaaaga ctggtacacg ctgataaaca ctatcatcaa cggttcagct
    35461 tctgaagcag atgccgcacg taagcaactg cgcgaagaac tgctggcgat tgcgccggtc
    35521 ttcggtcaga agccgtactt cctgagcgat gagttcagcc tggtcgattg ctaccttgct
    35581 ccgctgctgt ggcgtctgcc gcaactgggc atcgagttca gcggcccggg tgcgaaagag
    35641 ctgaaaggct atatgacccg cgtctttgag cgtgactctt tccttgcttc tttaactgaa
    35701 gcagaacgtg aaatgcgtct gggccggagt taatctgtat ggatttgtca cagctaacac
    35761 cacgtcgtcc ctatctgctg cgtgcattct atgagtggtt gctggataac cagctcacgc
    35821 cgcacctggt ggtggatgtg acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg
    35881 acgggcaaat cgtactcaac attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg
    35941 atgaggtgcg ctttaacgcg cgctttggtg gcattccgcg tcaggtttct gtgccgctgg
    36001 ctgccgtgct ggctatctac gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag
    36061 ctgcctacga tgaagatacc agcatcatga atgatgaaga ggcatcggca gacaacgaaa
    36121 ccgttatgtc ggttattgat ggcgacaagc cagatcacga tgatgacact catcctgacg
    36181 atgaacctcc gcagccacca cgcggtggtc gaccggcatt acgcgttgtg aagtaataca
    36241 aaacaggccc aggcggcctg ttttgtcttt ttaatgactt aataaaatag cgtgattgtg
    36301 gcaatcatat tcaccacgaa accgactgcc atggggatcg ctgtccgctt caccaccgca
    36361 aaaggcgaaa cgcctgcaat tcctgacgta acgaccacca ccgcagtaat cggcgaaacc
    36421 gcgcgcgcta gcgtcgtggc aaaatgcatc ggcataatca ttacaaccgc tggtacatgt
    36481 agtccggctg cgatattcgg aataagactg gcaaatgaca taaacggcgc attgccagag
    36541 cccatcacaa tggcacaaat ggcaatgact agcgccataa taatcatcac gccaataccg
    36601 cccagaccag aatgctccgc acccctgata accgcatcaa cagtgccaat cgtcgttaag
    36661 cctttcgcaa atatttcacc cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc
    36721 atgccgtcaa aaaacgcctg cacatcgtcc attgtttcgc gcaagttatg cttgcggaag
    36781 aactcaacaa acatcgtcac cgtcaaactt agtaacatca ccaccaccag atgcagttct
    36841 gacttcatca gcccgacgtg ggcgaggaac agcgagccga gcatcaggat taacggcatc
    36901 acaggtaaaa tggcgtaata gagcggcggg acattatcga gagttttttg ctctgcctgt
    36961 tcgtgattga tatttttatc ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc
    37021 gccaccgaga taatgacgca agaggcgacc ggaagctggt agtggaagaa gtatgtggca
    37081 attttcattc ccgcgacctg ggctgcaaaa atggagttcg tttccagaat cccccactca
    37141 atggacatcg tggttgcgat aaccgccacc gcagagaggc gacttactcc cagactcacc
    37201 agcgtcggaa ataaggtgac catcagcaac atacccagac cggaggcgct ggtaataaac
    37261 tgcgccatga tttggccgat gacgtaagtt gccgacagaa taatatacgg cgagcgaatg
    37321 agttttaacg ggcggcttag caagctcacc atcgcgcgac tggctcctat gcgctccatg
    37381 tagcgggcat aaccgcccac cgccataatc gacagcccca atccggcaat gcggttggac
    37441 agcatgcgca ggatttcatt ataaatgtcg acgatcaagt agccgctgct tttggcggga
    37501 tcgagtacac caccgaaccc taaccagact ccgcacatca tcaggaagac acctgcggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaac
//
LOCUS       DADWTH010000031        36607 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.031, whole genome shotgun sequence.
ACCESSION   DADWTH010000031 DADWTH010000000
VERSION     DADWTH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.031"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="JNY02_001650"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="JNY02_001650"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBB9433225.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /gene="adiY"
                     /locus_tag="JNY02_001651"
     CDS             2619..3380
                     /gene="adiY"
                     /locus_tag="JNY02_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBB9433226.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="JNY02_001652"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="JNY02_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBB9433227.1"
                     /translation="MSSDADAHKVGLIPVILMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="JNY02_001653"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="JNY02_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBB9433228.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="JNY02_001654"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="JNY02_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBB9433229.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="JNY02_001655"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="JNY02_001655"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBB9433230.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNY02_001656"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNY02_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBB9433231.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNY02_001657"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNY02_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBB9433232.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="JNY02_001658"
     CDS             complement(10310..11188)
                     /locus_tag="JNY02_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBB9433233.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNY02_001659"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNY02_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBB9433234.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="JNY02_001660"
     CDS             13815..14150
                     /locus_tag="JNY02_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBB9433235.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="JNY02_001661"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="JNY02_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBB9433236.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="JNY02_001662"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="JNY02_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBB9433237.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="JNY02_001663"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="JNY02_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB9433238.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="JNY02_001664"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="JNY02_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBB9433239.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="JNY02_001665"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="JNY02_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBB9433240.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVAEALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="JNY02_001666"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="JNY02_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBB9433241.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="JNY02_001667"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="JNY02_001667"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBB9433242.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="JNY02_001668"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="JNY02_001668"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBB9433243.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLVHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="JNY02_001669"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="JNY02_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBB9433244.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHVLEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="JNY02_001670"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="JNY02_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBB9433245.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="JNY02_001671"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="JNY02_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBB9433246.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="JNY02_001672"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="JNY02_001672"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBB9433247.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNRIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="JNY02_001673"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="JNY02_001673"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBB9433248.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="JNY02_001674"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="JNY02_001674"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBB9433249.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="JNY02_001675"
                     /pseudo
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="JNY02_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
     gene            complement(25844..26173)
                     /locus_tag="JNY02_001676"
     CDS             complement(25844..26173)
                     /locus_tag="JNY02_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433250.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="JNY02_001677"
     CDS             complement(26242..26874)
                     /locus_tag="JNY02_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB9433251.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRVAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="JNY02_001678"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="JNY02_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="JNY02_001679"
     CDS             29284..30804
                     /locus_tag="JNY02_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBB9433252.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="JNY02_001680"
     CDS             30801..31775
                     /locus_tag="JNY02_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBB9433253.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="JNY02_001681"
     CDS             31806..32747
                     /locus_tag="JNY02_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB9433254.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="JNY02_001682"
     CDS             32802..33485
                     /locus_tag="JNY02_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HBB9433255.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQGLMLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="JNY02_001683"
     CDS             33500..34360
                     /locus_tag="JNY02_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HBB9433256.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="JNY02_001684"
     CDS             34357..35358
                     /locus_tag="JNY02_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBB9433257.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="JNY02_001685"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="JNY02_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36607
                     /locus_tag="JNY02_001686"
     CDS             36352..>36607
                     /locus_tag="JNY02_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433258.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         8525 a        10223 c         9725 g         8134 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gattacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt catcctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgccgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctgggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccgcccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgaagcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatggttc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg tgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg tgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttcggtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aatgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccgccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcgtattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tacaggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gtaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagc gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagcc gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccactcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctcg ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgctgcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaggtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacagg
    33001 ggctgatgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatc
//
LOCUS       DADWTH010000032        36502 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.032, whole genome shotgun sequence.
ACCESSION   DADWTH010000032 DADWTH010000000
VERSION     DADWTH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.032"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(156..1391)
                     /locus_tag="JNY02_001687"
     CDS             complement(156..1391)
                     /locus_tag="JNY02_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBB9433259.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="JNY02_001688"
     tRNA            complement(1538..1614)
                     /locus_tag="JNY02_001688"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="JNY02_001689"
     tRNA            complement(1657..1743)
                     /locus_tag="JNY02_001689"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="JNY02_001690"
     tRNA            complement(1764..1839)
                     /locus_tag="JNY02_001690"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="JNY02_001691"
     tRNA            complement(1898..1974)
                     /locus_tag="JNY02_001691"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="JNY02_001692"
     CDS             complement(2077..3462)
                     /locus_tag="JNY02_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB9433260.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="JNY02_001693"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="JNY02_001693"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBB9433261.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="JNY02_001694"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="JNY02_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBB9433262.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIFRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="JNY02_001695"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="JNY02_001695"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBB9433263.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTWMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="JNY02_001696"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="JNY02_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBB9433264.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="JNY02_001697"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="JNY02_001697"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBB9433265.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="JNY02_001698"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="JNY02_001698"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBB9433266.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10723)
                     /gene="rfbA"
                     /locus_tag="JNY02_001699"
     CDS             complement(9860..10723)
                     /gene="rfbA"
                     /locus_tag="JNY02_001699"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBB9433267.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTDTHDSLIEASTFVQTVEKRQGFKIAWRNGWLDDEGVKRAASSLAKTGYGQYL
                     LELLRARPRQY"
     gene            complement(10742..11809)
                     /gene="rffG"
                     /locus_tag="JNY02_001700"
     CDS             complement(10742..11809)
                     /gene="rffG"
                     /locus_tag="JNY02_001700"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBB9433268.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGHYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11809..13071)
                     /gene="wecC"
                     /locus_tag="JNY02_001701"
     CDS             complement(11809..13071)
                     /gene="wecC"
                     /locus_tag="JNY02_001701"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBB9433269.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13068..14198)
                     /gene="wecB"
                     /locus_tag="JNY02_001702"
     CDS             complement(13068..14198)
                     /gene="wecB"
                     /locus_tag="JNY02_001702"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBB9433270.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14254..15300)
                     /gene="wzzE"
                     /locus_tag="JNY02_001703"
     CDS             complement(14254..15300)
                     /gene="wzzE"
                     /locus_tag="JNY02_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBB9433271.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15312..16415)
                     /gene="wecA"
                     /locus_tag="JNY02_001704"
     CDS             complement(15312..16415)
                     /gene="wecA"
                     /locus_tag="JNY02_001704"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBB9433272.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16655..17914)
                     /gene="rho"
                     /locus_tag="JNY02_001705"
     CDS             complement(16655..17914)
                     /gene="rho"
                     /locus_tag="JNY02_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBB9433273.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17999..18100)
                     /gene="rhoL"
                     /locus_tag="JNY02_001706"
     CDS             complement(17999..18100)
                     /gene="rhoL"
                     /locus_tag="JNY02_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBB9433274.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18241..18570)
                     /gene="trxA"
                     /locus_tag="JNY02_001707"
     CDS             complement(18241..18570)
                     /gene="trxA"
                     /locus_tag="JNY02_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBB9433275.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18701..19966
                     /gene="rhlB"
                     /locus_tag="JNY02_001708"
     CDS             18701..19966
                     /gene="rhlB"
                     /locus_tag="JNY02_001708"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBB9433276.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20100..21584
                     /gene="gppA"
                     /locus_tag="JNY02_001709"
     CDS             20100..21584
                     /gene="gppA"
                     /locus_tag="JNY02_001709"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBB9433277.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21631..23652)
                     /gene="rep"
                     /locus_tag="JNY02_001710"
     CDS             complement(21631..23652)
                     /gene="rep"
                     /locus_tag="JNY02_001710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBB9433278.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23739..24020
                     /gene="ppiC"
                     /locus_tag="JNY02_001711"
     CDS             23739..24020
                     /gene="ppiC"
                     /locus_tag="JNY02_001711"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBB9433279.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24066..25541)
                     /gene="ilvC"
                     /locus_tag="JNY02_001712"
     CDS             complement(24066..25541)
                     /gene="ilvC"
                     /locus_tag="JNY02_001712"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBB9433280.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMVDWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25691..26581
                     /gene="ilvY"
                     /locus_tag="JNY02_001713"
     CDS             25691..26581
                     /gene="ilvY"
                     /locus_tag="JNY02_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBB9433281.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26578..28122)
                     /gene="ilvA"
                     /locus_tag="JNY02_001714"
     CDS             complement(26578..28122)
                     /gene="ilvA"
                     /locus_tag="JNY02_001714"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBB9433282.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28125..29975)
                     /gene="ilvD"
                     /locus_tag="JNY02_001715"
     CDS             complement(28125..29975)
                     /gene="ilvD"
                     /locus_tag="JNY02_001715"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBB9433283.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30040..30969)
                     /gene="ilvE"
                     /locus_tag="JNY02_001716"
     CDS             complement(30040..30969)
                     /gene="ilvE"
                     /locus_tag="JNY02_001716"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBB9433284.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30989..31252)
                     /gene="ilvM"
                     /locus_tag="JNY02_001717"
     CDS             complement(30989..31252)
                     /gene="ilvM"
                     /locus_tag="JNY02_001717"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBB9433285.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31249..32895)
                     /locus_tag="JNY02_001718"
     CDS             complement(31249..32895)
                     /locus_tag="JNY02_001718"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBB9433286.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32898..32948)
                     /locus_tag="JNY02_001719"
     CDS             complement(32898..32948)
                     /locus_tag="JNY02_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433287.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33034..33132)
                     /gene="ilvL"
                     /locus_tag="JNY02_001720"
     CDS             complement(33034..33132)
                     /gene="ilvL"
                     /locus_tag="JNY02_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBB9433288.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33484..35004
                     /locus_tag="JNY02_001721"
     CDS             33484..35004
                     /locus_tag="JNY02_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBB9433289.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35029..35367)
                     /gene="maoP"
                     /locus_tag="JNY02_001722"
     CDS             complement(35029..35367)
                     /gene="maoP"
                     /locus_tag="JNY02_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBB9433290.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35486..36325
                     /gene="hdfR"
                     /locus_tag="JNY02_001723"
     CDS             35486..36325
                     /gene="hdfR"
                     /locus_tag="JNY02_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBB9433291.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLSGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGSLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36421..36496)
                     /locus_tag="JNY02_001724"
     tRNA            complement(36421..36496)
                     /locus_tag="JNY02_001724"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36461..36463),aa:Trp,seq:cca)
BASE COUNT         8919 a         9988 c         9194 g         8401 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccaccctgcc aagtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttatgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaaa attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccac gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cacgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcataacggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atcttaaagc cctggcgttt ttctactgtc tgcacaaaag
    10021 tgctggcctc aatcaggctg tcgtgagtgt cggtatccag ccaggcaaat ccgcgcccga
    10081 gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca
    10141 gttcaccgcg ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga
    10201 aataaagccc ggtcaccgcc cagtttgact tcggctgttt tggcttttct tcaagcgaga
    10261 tagcgcggaa attgtcgtca aattccacca cgccaaagcg ttccgggtcc atcacctgat
    10321 agtcaaaaac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc
    10381 cctgaccgaa gaagatgtta tcgcccagca ccagacaaga aggttcacca ttaaggaagg
    10441 tttcaccgat gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcatattcca
    10501 gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg
    10561 gcgtagtgat gatgagaatt tcgcggatac cggccagcat cagcaccgac agcggatagt
    10621 aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat
    10681 gcaatcgggt accggaaccg cccgccagga taataccctt catgtttgcc tccgctgaaa
    10741 attagccttt cagacctaaa cgctcaccct gatagctgcc gtcctgcacc tgcttccacc
    10801 agctttcatt agccagatac cactgaaccg ttttacgcat tccactttca aaggtttcct
    10861 gcggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat
    10921 gccccggacg gtcagcgaca aaggtgatca ggtcacgata gtgcgccacg ccgtgcggct
    10981 tgttcgaagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac
    11041 gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat
    11101 acagcgcgcg ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat
    11161 ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg
    11221 gaaagtggta atgaccgtag ttattcgagc agttggtgat cagcgtcggc agaccgtagg
    11281 tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc
    11341 tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata
    11401 cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat
    11461 tccagtaagc ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa
    11521 acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac
    11581 agtctggttg atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt
    11641 tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt
    11701 aggtcagctt atcgaccact accaccgcat cgctcgtttc gttgatgata taacgcacca
    11761 gcgccgagcc aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac
    11821 tcctttggca tcgacgacat actgctgatg gacattgtcg ccattgataa ctttgaactg
    11881 actatgatcg accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc
    11941 cagagtacaa agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag
    12001 cgtttcgccg ctgtgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg
    12061 caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc
    12121 gcgtttatcg gtagcagcaa ggcagtcggc aaccgccgct ttcacctgat cgataaccca
    12181 gaacggtttg tgatcgttaa cttcgcgcgc ggtacggata agccgcgcct gctgggggtt
    12241 ctgtgccacg ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg
    12301 ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat
    12361 cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa
    12421 gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc
    12481 ttcgaggaaa attttgtaca attcgctggc gcgggccgaa caaaccggcg tcataccacc
    12541 aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaata cacgttccgg
    12601 gcagtaagca atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tcagatccgg
    12661 gcgcatctct gctaaccatt cagccatctt ctcggttgac cccaccggcg aggtggattc
    12721 aaggatcacc agggcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac
    12781 gtaggtcata tctggctcat gatcgccttt aaacggcgta ggtacagcaa tcagccaggc
    12841 atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac
    12901 tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc
    12961 gtgttggttg atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt
    13021 aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc
    13081 cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta
    13141 tgggcgcggc tcatagcttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg
    13201 acaattcgct gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga
    13261 cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga
    13321 atgccgcctg agtcagtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa
    13381 tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc
    13441 tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg
    13501 gcgatgtctg ccagcgcgtg gcagatttct tcaaagccac gaccgaaact ctcacgccta
    13561 tgaccggtca ccagaatcat ctttttatcg ggggcgataa acgggtaatt tgccgccagt
    13621 tctgaacgca gcgtgtcgct gctcatcacc tggtcacgca cccataacag tgcatcaatg
    13681 actgtattac cggtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc
    13741 cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta
    13801 gcctcttccg gccacggcga atagagatcg cccgtgcgca gaccagcctc aacgtgacca
    13861 acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt cgtatcgccg
    13921 tgaaccagca caacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc
    13981 cgacaggtta tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt
    14041 acaatggaaa agagtttcag tacctgatca agcatctccc gatgctgcgc agtgacgcaa
    14101 actttagcct caaaaaaagg atcttttgcc aacgcatgca ccaacggagc catcttgatg
    14161 gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg
    14221 gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc gggttaatgc
    14281 gacaccagcc ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg
    14341 tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg
    14401 cggatccagg gttggaccaa cattcagggt gtttaacatg gctcgattct gatcatagtc
    14461 gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg
    14521 cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg
    14581 cgaaatatta tgctgctcag caattttcag cgcctgctca atgctgttca tccggcggtc
    14641 gtagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct ggatggtgcg
    14701 tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa
    14761 agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac
    14821 gctgtcattg accgcgcgag taaagtctcc ggggataaac tggatgttgt taatcatttc
    14881 atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt
    14941 ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata
    15001 ggcttcgtcc atgaccgatg gttgatcggc agaagccatg tttgaacgga catccaggtt
    15061 acgcaaaaat tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt
    15121 aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg cgattaacgc
    15181 aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc
    15241 acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat
    15301 cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt
    15361 tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg
    15421 aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct
    15481 gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctga
    15541 cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctggcggtc aggagagaat
    15601 gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg
    15661 gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg
    15721 agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact
    15781 ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc
    15841 atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt
    15901 gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg
    15961 aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc
    16021 accatctccc aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc
    16081 atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa
    16141 cgatcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca
    16201 tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc
    16261 cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa
    16321 ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata
    16381 ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat
    16441 tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc
    16501 agccctgtgc aaaaattaac catttgatat gcaaattgca taacatgcca gcaaattcca
    16561 ggaattttcc taacatcgcc agcgcgacat taagattaca gatataaaag caaaacgcca
    16621 cgtaaacacg tggcgttttt ggcataagac caatttatga gcgtttcatc atttcgaaga
    16681 aatcgtcatt ggtcttggtc atggccagtt tattaatgag gaattccatt gcatcgattt
    16741 cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg
    16801 tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt
    16861 tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt
    16921 aaataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc
    16981 tgccgccctc ttccacgtta cgcgccgcac caaagaagcg tttcggacga tgaagggcat
    17041 tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacagtg ttgtaagcgc
    17101 gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc
    17161 gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa
    17221 aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac
    17281 gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag
    17341 caatgttctg cagcagcata gttttaccgg ctttcggcgg tgccacaatc agaccacgct
    17401 gaccacgacc gataggtgat gccagatcca gtacgcgagc agttaaatct tcagtagaac
    17461 cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga
    17521 ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg
    17581 caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc
    17641 ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt
    17701 aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat
    17761 caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct
    17821 gcttacgcat gcgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag
    17881 aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg
    17941 taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc
    18001 atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag
    18061 acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg
    18121 ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt
    18181 agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc
    18241 ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg cgcccacttt
    18301 ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata
    18361 tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg
    18421 atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc
    18481 tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc
    18541 gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg
    18601 ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt caccggatac
    18661 gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac
    18721 agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa
    18781 agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg
    18841 tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac
    18901 gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccgcgtgc
    18961 cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc cacgccgacg cggaaccgct
    19021 ggcggaagct accggcctga agctgggcct ggcttacggt ggtgatggct atgacaaaca
    19081 gctgaaagtg ctggaaagcg gcgttgacat tctgattggc accacgggtc gtttaattga
    19141 ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtagtggtac tggacgaagc
    19201 cgatcgcatg tacgatctag gctttattaa agatatccgc tggctgttcc gccgtatgcc
    19261 gcctgcaaat cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga
    19321 actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac
    19381 gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct
    19441 gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca
    19501 ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac
    19561 aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct
    19621 ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca
    19681 cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac
    19741 aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt atgcattgaa
    19801 tttgcctgct attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga
    19861 cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg
    19921 tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac
    19981 tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga gcttactgta
    20041 gtaagtgatt cgggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta
    20101 tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc
    20161 tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaattaaa cgcaaagtgc
    20221 gtctggctgc tggcctgaat agcgaaaatg acctctctaa tgaagcaatg gagcgcggtt
    20281 ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg caaattcgcg
    20341 ttgtcgctac ggcgacatta cgacttgccg tcaatgcggg tgattttatt gccaaagcgc
    20401 aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagcg cgtctgattt
    20461 atcagggcgt tgctcacacc actggtggtg ccgatcagcg cctggtggtg gatataggcg
    20521 gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt
    20581 cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaagaaa
    20641 attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac
    20701 ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa
    20761 tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac
    20821 agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac
    20881 gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata
    20941 ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc
    21001 atctggccgt cgagcaggat attcgcagcc gtacactgcg taatattcaa cgccgcttta
    21061 tgatcgatat tgatcaggca cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg
    21121 tggaaaacga atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc
    21181 ttcatgaaat cggcctgagc gttgacttca aacaagctcc gcaacacgct gcttatctgg
    21241 tgcgtaacct ggatcttccc ggttttaccc ccgcacagaa gaaactgctg gcgacgctac
    21301 tgctcaacca gactaacccg gtcgatctct catcgctgca tcagcaaaat gccgtaccac
    21361 cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc tatcattttt gccagccgtc
    21421 gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga
    21481 cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa
    21541 accagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat
    21601 gcgatgctga cgcatcttct ccggccttga ttatttccct cgttttgccg ccatcatcgc
    21661 tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcac tgaccacttt
    21721 gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc
    21781 cgggcgcacc agttcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt
    21841 ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc
    21901 gatactgctc tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa
    21961 ctccagccct tttgacgcat ggagagtcat cagttgcacc tgatccagct cttcttcact
    22021 ctcaccacgc tccatcatgt cgcgtaaagt aaagcgcgtc accacctggg tgagcgtcat
    22081 cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac
    22141 gttcttcatg cgcatttcgg cagctttcgg gctgggcgat gtttcgtaca gccaggattc
    22201 ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg
    22261 ctggatttct gccaaccagt gagtgaagcg ggtcaatgct tcataaccac gtccgctaag
    22321 cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc
    22381 ccactcaccc agctttttca gcgtcgccgg gccaatctct cgcttcggcg tgttaacgat
    22441 acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc
    22501 cttgatttca ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt
    22561 ttgcatcagg aatttttcaa acacccgcga ctgatggtta ccgcgataaa gaatagcgta
    22621 atctttgtac tgcgttttat tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc
    22681 ggcttcatgt tcttcgttat tcgcgcttaa aacttttagc tctgtgccgt aacccagttc
    22741 ggagaacaga cgcttttcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt
    22801 caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca gcgccggaaa
    22861 atcctggctc agcagcacca ggttttgcgg acgtgcgccg cgccaggagt agatcgactg
    22921 gtcatcgtca cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc
    22981 atactggctg gtgttggtgt cctgatattc gtccaccagc aggtagcgaa ttttgttctg
    23041 ccagcgctcg cggacttctt cattgcgttg cagaagtagc gtcggcaata aaatcagatc
    23101 atcgaagtcg agcacattac aggctttcag gtgtgcatca tacagcccat aacaatgggc
    23161 aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc tgggacggtg ttttgagatc
    23221 gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt catcttcaat
    23281 cagcccttcg gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa
    23341 gttcgccttc atccccagcg ccgcatactc acgcttgatg atatccagcc ccagcgtgtg
    23401 gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc cgacacgctc
    23461 tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata
    23521 accgcacccg cggatcagat gggcgatttt attggtgata acacgagttt taccggaacc
    23581 cgcacccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg
    23641 gtttagacgc ataggaaaat cgctcaatga aagtcgggag gggaaaataa cagcgtggta
    23701 gtatagccag cctcatcctt accactcaag gcacgatcat ggcaaaaaca gcagcagcac
    23761 tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgtgcag attaagaacg
    23821 gtgccgattt cggcaagctg gcgaagaaac actccatttg cccatcaggc aaacgcggcg
    23881 gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa gttgtgttct
    23941 cttgtccggt actggagcca accggcccgc tgcacaccca gttcggttac cacatcatta
    24001 aggtgctgta ccgtaactaa tagcaaggcc ttctccggga gaaggccttg aactctgaat
    24061 cgctattaac ccgcaacagc aatacgtttc atatccgtca tatagccgcg cagtttctta
    24121 cctacttgct caatcgcatg gctgcgaatc gcttcattta catcacgcag ctgcgcgtta
    24181 tctaccgcac cttccggaat agctttaccc aaatcgcccg gttgcagttc tgccataaac
    24241 ggtttcagca gcggcacaca agcgtaagag aacagatagt taccgtactc ggcagtatca
    24301 gagataacca cgttcatttc atacagacgc ttacgggcga tggtgttggc aatcagcggc
    24361 agttcgtgca gtgattcata gtaagcagat tcttcgatga tgccggaatc aaccatggtt
    24421 tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca tcagtacgcc tttatcgaag
    24481 tactcctgct cgccgatttt gccttcatac tgcggcgcgg tttcgaaggc ggttttgccg
    24541 gtctcttcac gccaggtcag cagtttctta tcatcgttgg cccagtccac catcataccg
    24601 gaggagaatt ctccggagat gatgtcgtcc atatgtttct ggaacagcgg tgccatgatc
    24661 tctttcagct gttcagacaa cgcgtaagca cgcagtttcg ccgggttaga gagacggtcc
    24721 atcatcagtg tgataccgcc ctgcttcagc gcttcggtga tggtttccca accgaactga
    24781 attagttttt ctgcgtatgc cggatcggtg ccctcttcca ccagcttgtc gaagcacagc
    24841 agcgagccag cctgcaacat accgcacagg atggtttgct cacccatcag gtcagatttc
    24901 acttccgcaa cgaaggacga ttccagcacg cccgcacggt gaccaccggt tgctgccgcc
    24961 catgctttag cgatcgccat gccttcgcct ttcggatcgt tttccgggtg aacggcaatc
    25021 agcgtcggta cgccgaatcc acgtttgtac tcttcacgca cttcggtgcc agggcatttc
    25081 ggcgcaacca tgactacggt gatgtcttta cggatctgct cgcccacttc gacgatgttg
    25141 aaaccgtgcg agtaacccag cgccgcgccg tctttcatca gtggctgtac ggtgcgcact
    25201 acatcagagt gctgcttgtc cggcgtcagg ttaaccacca gatccgcctg tgggatcagt
    25261 tcttcgtaag tacccacttt aaaaccattt tcggtcgctt tacgccagga cgcgcgcttc
    25321 tcggcaatcg cttctttacg cagagcgtag gagatatcga gaccagaatc acgcatgttc
    25381 agaccctggt tcagaccttg tgcgccacag ccgacgatga ctactttttt accctgaagg
    25441 tagctcgcgc catcggcgaa ttcatcgcgc cccataaagc gacatttgcc cagctgtgcc
    25501 agctgctgac gcagattcag tgtattgaag tagttagcca tggtgattcc tcgtgatgtt
    25561 gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt cactatatga caggaaattt
    25621 attgcggaaa ttgatatatt cacaacgtca cattgcaatt tctgcaacgt caacattgag
    25681 ggctgcgcca ttggatttac gcgatctgaa aaccttcctg catctggcgg aaagccgcca
    25741 ttttggccgc agcgcgcggg cgatgcacgt tagcccgtcc acgctctcac ggcagattca
    25801 gcgcctggaa gaagatctcg gccagccgct gtttgtgcgc gataaccgca cggtgacgct
    25861 gactgaagcg ggcgaagaac tgcgcgtttt cgcccagcaa acgctgttgc agtatcagca
    25921 gctgcgccac accatcgacc agcaagggcc gttgctctat ggcgaattac atatcttctg
    25981 ctcggtaacc gccgcttaca gccatctgcc gccgattctg gatcgcttcc gcgcggaaca
    26041 cccgtcggtg gagatcaaac tcactaccgg tgatgcggca gatgcaatgg aaaaagtcgt
    26101 cacaggtgaa gctgatctgg cgattgccgg gaagccagag actctgcccg gcgcggtggc
    26161 gttttcgatg ctggagaatc tggcagtggt gttgattgca cccgcattgc cctgcccggt
    26221 gcgtaatcag gtttctgtcg aaaagccgga ctggtcaacg gtaccgttta tcatggccga
    26281 tcaggggccg gtgcgccgcc gcattgagtt gtggttccga cgcaataaaa tcagtaaccc
    26341 gatgatttac gccacggttg gcgggcatga agcgatggta tcgatggtgg cgctcggctg
    26401 tggcgtggca ttgttgccgg aagtggtgct ggaaaacagc cccgaaccgg tgcgtaaccg
    26461 cgtgatgatt ttagagcgca gcgatgagaa aacgccgttt gagctgggcg tgtgtgcgca
    26521 gaaaaagcgg ctacatgagc cgctaattga ggcattctgg aagattttgc cgaaccatta
    26581 acccgccaaa aagaacctga acgccgggtt attggtttcg tcgtggcaat cgtagcccag
    26641 ctcattcagc cgggtttcga aatccggttc atggtcgcca agttcgaacg ccgccagtac
    26701 gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc agtgcgtacc
    26761 cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc gggaattcga agctgtagag
    26821 gcgttcctgc aacggatgtg acggacgccc gccgaccata tagcgcacat gcaacttcgc
    26881 catttcgtcg tcggagagat ccaccacgct gtagccgccg tcgttgagca tctgcaaaat
    26941 ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc aggcgttttt
    27001 ggcatcggca aaacggtagt tgaactcggt gaccgatcgc ccgccaagca gttggcagaa
    27061 tttgaggaag ctgccttttt cttccggaat ggtcaccgcc aacaacgctt cacgctgttc
    27121 gcccagttcg cagcgttccg agacgtagcg caggccgtgg aagttcacgt tggcaccgga
    27181 aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt ttttcattcc
    27241 cgccagcgcc agcgcgccag agggttccgc caccgcacgc acatcttcga ataaatcctt
    27301 catcgccgca cagatcgcat cgctatcgac ggtgatgatg tcgtcgagat actcctggca
    27361 taaacggaag gtttcgtcac cgatgcgttt taccgcgaca ccttcggcga acagtcccac
    27421 gcgtggcaga tcaaccggat gacccgcatc cagcgctgct ttcaggcagg cggagtcttc
    27481 cgcttctacg gcgatcactt tgatttgcgg catcagttgt ttgatcagca ccgctacgcc
    27541 cgccgccaga ccgccgccgc cgactggcac aaatacgcgg tcgagatggg agtcctgctg
    27601 gagcagttcc agcgccagcg tgccttgccc ggcaatcacc atcggatggt cgaacggcgg
    27661 cacccaggtg aacccctgct gctgtgacag ttcgatcgct ttagctttcg cttcgtcaaa
    27721 gttcgcgcca tgaagcagca cttcgccgcc gaatccacgt accgcatcga ctttgatgtc
    27781 ggcggtggcg gttggcataa cgatcagggc cttcacgcct aaccgcgcag aagaaaacgc
    27841 gacgccctgc gcgtggttac ccgcagaagc agtgatcacg ccgtgcgctt tttgttcttc
    27901 cgtcaggccc gccatcatgg cgtatgcgcc gcgcagctta aagctgtgca ctggctggcg
    27961 atcttcgcgc ttcaccagaa tgacgttatc aagacgcgac gacagttttt ccattttttg
    28021 tagcggcgta acctgcgccg cctcgtaaac cggcgcgcgc agcactgctc ttaaatattc
    28081 ggcaccttcc ggagcaccgg acaggggttg cgagtcagcc attattaacc ccccagtttc
    28141 gatttatcgc gcaccgcacc tttgtcggcg ctggttgcca ggctggcgta ggcgcgcagg
    28201 gcaaaggaaa cctgacgttc acggtttttc ggcgtccagg ctttgtcacc ccgagcttcc
    28261 tgcgcttcac gacgcgccgc cagttcggca tcgcttacct gtaactgaat gccacggttc
    28321 gggatgtcga tagcgatcag gtcaccatct tcaatcagac caatgctgcc gccgcttgcc
    28381 gcttccggtg agacgtggcc gatggaaaga ccagaggtgc cgccagagaa acgaccgtcg
    28441 gtgatcagcg cacaggcttt accgagaccc attgatttca ggaagctggt tgggtagagc
    28501 atttcctgca tgccagggcc gcctttcggg ccttcatagc ggatcaccac cacatcgccc
    28561 gcgacaactt tgccaccgag gatcgcttct accgcgtcgt cctggctttc gtacactttc
    28621 gccgggccgg tgaatttgag gatgctgtca tcgacgcccg cggttttcac gatgcagccg
    28681 ttttcagcaa aattaccgta gagcaccgcc aggccgccgt ctttgctgta ggcgtgttcc
    28741 agcgagcgga tacagccgtt tgcgcgatcg tcatcgagag aatcccaacg gcaatcctgc
    28801 gagaatgcct gtgtagtacg aatgcccgca gggcctgcgc ggaacatatt ttttaccgcg
    28861 tcatcctggg tcagcataac gtcgtattgt tccagagttt gcggcaacgt caggccaagt
    28921 acgtttttca catcacggtt cagtaacccc gcgcgatcca gttcaccgag aataccgata
    28981 acaccaccag cacggtgaac atcttccata tggtattttt gggtgctcgg cgcaactttg
    29041 cataactgcg ggactttacg cgaaagtttg tcgatatcac tcatggtgaa gtcgatttcc
    29101 gcttcctgcg ccgccgccag caggtgaagt accgtgttgg tagagccgcc catcgcgata
    29161 tccagcgtca tggcattttc aaacgccgct ttgctagcaa tattgcgcgg cagtgcactt
    29221 tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa tgcgtttacc ggcattgagg
    29281 aacaactgct tacggtccgc gtgggttgcc agcagcgagc cgttgcccgg ctgcgacagg
    29341 cccagcgctt cggtcaggca gttcattgag ttagcggtaa acatcccgga gcaggagccg
    29401 caggtcggac acgcggaacg ttcaacctga tcgctctggg agtcagaaac tttcgggtct
    29461 gcgccctgga tcatcgcatc aaccagatcg agtttgatta tctgatcgga cagtttggtt
    29521 ttcccggcct ccatcgggcc gccggaaaca aagatcaccg gaatattcag gcgcagggaa
    29581 gccatcagca tccccggggt gattttgtcg cagttagaga tgcagaccat ggcgtcggcg
    29641 cagtgggcgt tgaccatata ctcaacggaa tcagcgatca gttcgcgaga tggaagtgaa
    29701 taaagcatcc ccccgtggcc catggcaatc ccatcatcca ccgcaatggt gttgaactct
    29761 ttggcaacgc cgccagccgc ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga
    29821 tggacgtgac ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat cggcttgcca
    29881 aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc catattacga
    29941 ccatgagtgg tggtggcgga acggtactta ggcatacttt atttactccc agtgtctgtc
    30001 tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt tattgattaa cttgatctaa
    30061 ccagccccat ttatcttcgg tttcgccagt gaagaggccg aagaaggctt gctgaatgcg
    30121 tttggtaacc gggccacaac ggccttcgcc aatctgaata ccgtctacgc tgcgcactgg
    30181 cgtgatttct gccgccgtac cggacataaa gacttcatcc gccaagtaca gggattcgcg
    30241 cgacagcacc tgctcacgca cttcaattcc cagctctttc gccagtttga tgatggcatc
    30301 acgggtaata cccggcagcg cggaggaggt gaacggtggg gtgaacagca caccatcttt
    30361 cacttcaaac aggttttcgc ctgcgccttc agagatataa ccgttcacat ccagcgcgat
    30421 accttcctga taaccgtggc ggcgcgcttc gctacccacc agcagggaag agagatagtt
    30481 accaccggct tttgccgcag ttgggatggt gtttggtgct gcgcggttcc aggaggaaac
    30541 catcgcatcg atcccctgct ccagcgcttc tgcgcccaga tacgctcccc acgggaaagc
    30601 ggcgataatc acatcggttg agtatcccgc tggcgggttt actcccatgc caacatcacc
    30661 gacgaagatc agcggacgga tataggcgct ggtgagattg tttttgcgga tcacgtcacg
    30721 acaagcttcc atcagctcat caatgctctg tgaaaccggg aagcgataga ttttggcgga
    30781 gtcatgcaga cgctgcatat gctcacgatg gcggaataca accggtcctt tgtgtgagtc
    30841 gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg
    30901 caccttcgcg tcttcccagc gaaccatctc cccattgaac caaatgtaat cagctttctt
    30961 cgtggtcatt tttatattcc ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc
    31021 tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt taactgacta aacagtaagt
    31081 cgaccgaccg tgggctggca acggtcaatt cgatatttat attttgtgca tcgctggcgg
    31141 cagccatatt cattgagcag acgtggaaac cacgatgacg caccacgcgt aaaacacgtt
    31201 ctaaggtttc cggattgaag cgagccgata cattgacctg atgttgcatc atgataattt
    31261 ctccaacatt tctgaattac tggcacctgg cggcaccagc ggccagacgt tctcaagttc
    31321 gtcgattgag acatgaagca ggtatggccc atcactgttc agcatggtgt cgagtgccgc
    31381 ttcaacctgg tctttacggg taatgtgttg gccagggatg ccgaaggcgc tggctaacat
    31441 gaggaaatcg gggttatcgg taagggtggt ttcgctgtat cgttcctgaa aaaacagttg
    31501 ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt aagacgattt tcaacggtaa
    31561 ctgcttgcgt tttacggtgc ccagctcttg cacattcatc atgaaagagc cgtcaccgga
    31621 gatacagaca acggtatcgt tcggtcgcgc gacttgtgcg ccaactgccg ccggtaaacc
    31681 aaaacccatg gtacctaagc cgctggaggt gatgaaattt tccgggcgag tgtgggcgat
    31741 gtgctgcgca gcccacatct ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg
    31801 tttacgatcc gacagttgtt ttaacaacaa cggagcgtag atagcgtcac cgggatggtc
    31861 gtaacgccag gaatgttcat cacgcagctg cgcgcagtgt tgctgccagt cattgatatt
    31921 taacggctgc tgtaatgctg gtaacagagc atttaaatca ccttgtaatg ccacatgtgc
    31981 ctgacgcagc ttgttcattt ctgccgggtc gatatccata tggataacac tggcgtgtgg
    32041 tgcgaaggtg ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag
    32101 caggtcacac tcctgcaccg cgaagtttgc cgctttggtt ccgtgcatcc ccagcatgcc
    32161 cagatagtac ggataatctg cttctactgc gcccagccct ttcagcgtac aggtggcagg
    32221 catttttgtg gcagcgagaa attcacgcaa agccggaact gcctgcgcca tccccacgcc
    32281 accgccaacg tacagcatcg gtttttgcgc ttttgccagc atctggcgcg cttgctcgac
    32341 ttcggcatgt gggaaagtca cttcgttttc aacggtggtg aaccacggtt ccagatcgcc
    32401 gctggctaac tggatatctt ttgggatatc gaccagaacc ggaccaggac gacctgagct
    32461 ggcaacgtcg aatgcttcag ccatgatgcg cggcaactct tccagagact gcaccagaaa
    32521 gctgtgcttg gtacaggcta acgacaatcc caggacatcc acttcctgaa atgcgtcggt
    32581 gccgataaac ggtgcggaca cctgaccagt gatggcaacg acagggatgg aatctaacag
    32641 tgcgtccgca agcccggtta tcaggttggt tgcgcccgga ccagacgtgg cgatacatac
    32701 gccagtttta ccggtagcac gggcataacc gatagccgct attgccgcac cctgctcatg
    32761 tcggcacagc aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg gcataattgc
    32821 gccacccgga taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat gtaccaccca
    32881 ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg
    32941 ttattcattg tctgctcctc ggttatgttt ttaaggtcaa aaaaaccccc ggacctttcg
    33001 gtgcgggggt cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc
    33061 cccgcacggt gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag
    33121 aagggctgtc attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca
    33181 tagattttgc gaatagcaca agatattttt gggcctattt ttatgatggc tttaataatt
    33241 tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaattc aatttcagta
    33301 taaatgttgt tcccttcctc gtagttcatc ctttgtccgc tgcatttttg cgagcatcat
    33361 tccaccgcca gaacaactca acttcacttg cacaaataaa caggaatcag ccactaaaat
    33421 tcacgaatta gcgggctaca gcagcgatat cttatgcaca taatggtgac tcaaggaggg
    33481 tttatgtcac tgtcaattgt tcatacccgc gcagccctgg aagtaaatgc gcccccaatc
    33541 actgttgagg tacatatcag taaaggtcta cccggcttaa cgatggtggg cttaacagaa
    33601 acaacggtga aagaagctcg cgatcgcgtg cgcagcgcca ttatcaatag cggatatgaa
    33661 tatccggcga aaaaaatcac catcaacctt gctccagccg atctgccaaa agaaggggga
    33721 cgatatgatt tacctatcgc cattgcgttg ctggcggcct cagaacagct tacagccaat
    33781 aagttagatg aatatgaatt agtcggagaa ctagcgctta caggcgctct gcgtggcgtt
    33841 cccggtgcaa tctccagtgc aactgaagct attaagtcgg gcagaaaaat tatcgtcgcg
    33901 aaagataacg aagatgaagt ggggctaatt aacggtgaag gatgcctgat agccgatcat
    33961 ctgcaggctg tctgtgcgtt tctggaaggt aagaacactc tcgaacgccc gaaaccaact
    34021 gatgcagtat cccgggcgct acaacatgat ctcagtgatg ttgtcggtca ggagcaagga
    34081 aaacgaggac tggaaattac cgccgctggt gggcacaacc ttttactgat tgggccgccg
    34141 ggaacaggta aaacaatgct cgccagccgt attaatggcc tgttgccaga tttaagcaat
    34201 gaagaggcac tggagagcgc tgcgatatta agtctggtaa atgctgaatc agtacaaaaa
    34261 caatggcggc agcgcccgtt ccgctcacct catcacagtg cgtcgttaac tgcgatggtg
    34321 ggcggtggtg caattccagg gccaggtgaa atttcgttgg cgcataacgg cgtgcttttt
    34381 cttgatgagc tacctgaatt tgaacggcgt acactggatg ccttgcgaga gccgattgaa
    34441 tccgggcaga tccatctttc acgcacacga gcaaaaataa cctatccagc ccgtttccag
    34501 cttgttgcgg cgatgaatcc cagccctacc ggacattatc agggaaacca taaccgctgc
    34561 acgccagaac agacattacg ttatctcaac cggctctcgg ggccctttct cgaccgcttc
    34621 gatctctcac tggagatccc attaccaccc cccggcattt tgagtaaaac ggtagtgccg
    34681 ggagaaagca gcgccaccgt taaacaacgc gtaatggccg ccagagagcg ccaatttaag
    34741 cggcagaata aactgaatgc ctggctggat agtccggaaa tacgccaatt ctgcaaactt
    34801 gagagcgaag atgcgatgtg gttggaagaa acgctgatcc atctggggtt atcgattcgt
    34861 gcctggcagc ggttactgaa agttgcacga accattgctg atattgatca gtctgacatt
    34921 atcacacgtc agcatttgca ggaggcagtt agctatcgtg cgattgaccg tttgctcatc
    34981 catctgcaaa aactactgac ataaaaaaag ggcatttcgc cctttttatt aatcgtcaga
    35041 atcggtgtag tcttcagcac cttcaacctg cggtttaccg ccggaaaggg tgtgaaaacg
    35101 ttttggacgc ttaatacgcg tcatatactt ggaccacacg cgttctgctt ctgtcactgg
    35161 ctcacgttcg ccacggcata ctgctacgaa gagtttctct tcctcggtaa ccggctcgcg
    35221 tttgccaaga tccaactcat tgaaggcata accatgacgc tcaagcagtt gtgcctcttt
    35281 gatggtgaaa tcaccatgac gagagaatcc acgtggataa tgtttattgt cgaaatatcg
    35341 attagtcgtc gtaaagcttt ccgccatcct gcacgctcct aattctttga ccgagctagt
    35401 tatggcgcgg agtattagtt acgcttgaca gagtgtaaaa caaaacattt aaatcataac
    35461 gacaaataat tttgcggaga gcactgtgga tacggaattg ttaaaaactt tcctggaagt
    35521 tagccgaacg cgtcactttg gtcgagcggc tgaatcgctc tatctgaccc agtcagcagt
    35581 gagctttcga atcagacaac tggaaaatca actgggtgtg aaccttttca cccgccacag
    35641 aaacaatatc cgtttaaccg ctgccggtga aaaactactg ccttatgcag aaacgctcat
    35701 gagcacctgg caggccgccc gcaaggaggt ggcgcatacc tcacgacata acgagttttc
    35761 tatcggtgcc agcgcctcgt tgtgggaatg tatgcttaat cagtggttgg gacgcttgta
    35821 tcaaaatcag gatgcccata caggcttaca gttcgaagcg cgaattgccc aacggcagtc
    35881 tctggtaaaa cagctgcatg aacgccagct tgatcttctt attaccactg aagcgcccaa
    35941 aatggacgaa tttagtagtc agttgctggg gtatttcact ttagcgcttt ataccagtgc
    36001 cccttctaaa ctaaagggag atcttaacta tctgcgactg gagtgggggc cagattttca
    36061 acagcatgag gcaggtttga gcggtgctga cgaagttccc attctgacaa ccagttctgc
    36121 tgaactggca cagcaacaga ttgcgatgct taatggttgc acctggctac ccgtcagctg
    36181 ggcgcgtaaa aaaggcagcc tgcataccgt tgtcgatagc acaacacttt cacggccgct
    36241 ttatgccata tggctgcaaa atagcgataa aaatgcgttg attcgcgatc ttttgaaaat
    36301 taacgtgctg gatgaagtgt attaatctga atggctggca aggatgccgg tagaaggatt
    36361 tacttcggag aggattattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
    36421 tggcaggggc ggagagactc gaactcccaa cacccggttt tggagaccgg tgctctacca
    36481 attgaactac gcccctaatt ag
//
LOCUS       DADWTH010000033        35744 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.033, whole genome shotgun sequence.
ACCESSION   DADWTH010000033 DADWTH010000000
VERSION     DADWTH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.033"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..218)
                     /locus_tag="JNY02_001725"
     CDS             complement(<1..218)
                     /locus_tag="JNY02_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBB9433292.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRTLAEVY"
     gene            complement(399..1874)
                     /locus_tag="JNY02_001726"
     CDS             complement(399..1874)
                     /locus_tag="JNY02_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBB9433293.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKCFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3300)
                     /locus_tag="JNY02_001727"
                     /pseudo
     CDS             complement(2021..3300)
                     /locus_tag="JNY02_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="JNY02_001728"
     CDS             complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="JNY02_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBB9433294.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGITLAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5783..6529
                     /gene="mipA"
                     /locus_tag="JNY02_001729"
     CDS             5783..6529
                     /gene="mipA"
                     /locus_tag="JNY02_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBB9433295.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6619..7380
                     /locus_tag="JNY02_001730"
     CDS             6619..7380
                     /locus_tag="JNY02_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB9433296.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7431..8315)
                     /locus_tag="JNY02_001731"
     CDS             complement(7431..8315)
                     /locus_tag="JNY02_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBB9433297.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="JNY02_001732"
     CDS             complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="JNY02_001732"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBB9433298.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9736..10149
                     /gene="msrB"
                     /locus_tag="JNY02_001733"
     CDS             9736..10149
                     /gene="msrB"
                     /locus_tag="JNY02_001733"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBB9433299.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10191..10463
                     /locus_tag="JNY02_001734"
     CDS             10191..10463
                     /locus_tag="JNY02_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBB9433300.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10833..11909
                     /locus_tag="JNY02_001735"
     CDS             10833..11909
                     /locus_tag="JNY02_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBB9433301.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11936..13304
                     /locus_tag="JNY02_001736"
                     /pseudo
     CDS             11936..13304
                     /locus_tag="JNY02_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13321..14366
                     /locus_tag="JNY02_001737"
                     /pseudo
     CDS             13321..14366
                     /locus_tag="JNY02_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14387..15223
                     /locus_tag="JNY02_001738"
     CDS             14387..15223
                     /locus_tag="JNY02_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBB9433302.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15228..16176
                     /locus_tag="JNY02_001739"
                     /pseudo
     CDS             15228..16176
                     /locus_tag="JNY02_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16186..17166
                     /gene="ydjG"
                     /locus_tag="JNY02_001740"
     CDS             16186..17166
                     /gene="ydjG"
                     /locus_tag="JNY02_001740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBB9433303.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDPALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17303..18061
                     /locus_tag="JNY02_001741"
     CDS             17303..18061
                     /locus_tag="JNY02_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB9433304.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18179..19537
                     /locus_tag="JNY02_001742"
     CDS             18179..19537
                     /locus_tag="JNY02_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9433305.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19630..20271)
                     /gene="pncA"
                     /locus_tag="JNY02_001743"
     CDS             complement(19630..20271)
                     /gene="pncA"
                     /locus_tag="JNY02_001743"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBB9433306.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20282..21298)
                     /gene="ansA"
                     /locus_tag="JNY02_001744"
     CDS             complement(20282..21298)
                     /gene="ansA"
                     /locus_tag="JNY02_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBB9433307.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(21465..23321)
                     /gene="sppA"
                     /locus_tag="JNY02_001745"
     CDS             complement(21465..23321)
                     /gene="sppA"
                     /locus_tag="JNY02_001745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBB9433308.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23482..24033
                     /locus_tag="JNY02_001746"
     CDS             23482..24033
                     /locus_tag="JNY02_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBB9433309.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24150..25193
                     /gene="selD"
                     /locus_tag="JNY02_001747"
     CDS             24150..25193
                     /gene="selD"
                     /locus_tag="JNY02_001747"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBB9433310.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSKMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25198..27159
                     /gene="topB"
                     /locus_tag="JNY02_001748"
     CDS             25198..27159
                     /gene="topB"
                     /locus_tag="JNY02_001748"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB9433311.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIEQDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27286..28326
                     /locus_tag="JNY02_001749"
     CDS             27286..28326
                     /locus_tag="JNY02_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBB9433312.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMRLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28443..29786)
                     /gene="gdhA"
                     /locus_tag="JNY02_001750"
     CDS             complement(28443..29786)
                     /gene="gdhA"
                     /locus_tag="JNY02_001750"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBB9433313.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARAITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30022..30294
                     /locus_tag="JNY02_001751"
     CDS             30022..30294
                     /locus_tag="JNY02_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBB9433314.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30260..30667)
                     /gene="nudG"
                     /locus_tag="JNY02_001752"
     CDS             complement(30260..30667)
                     /gene="nudG"
                     /locus_tag="JNY02_001752"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBB9433315.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30754..31374
                     /locus_tag="JNY02_001753"
     CDS             30754..31374
                     /locus_tag="JNY02_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBB9433316.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(31383..32690)
                     /gene="ynjE"
                     /locus_tag="JNY02_001754"
                     /pseudo
     CDS             complement(31383..32690)
                     /gene="ynjE"
                     /locus_tag="JNY02_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(32757..33410)
                     /locus_tag="JNY02_001755"
     CDS             complement(32757..33410)
                     /locus_tag="JNY02_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB9433317.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33410..34944)
                     /locus_tag="JNY02_001756"
                     /pseudo
     CDS             complement(33410..34944)
                     /locus_tag="JNY02_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34917..35744)
                     /locus_tag="JNY02_001757"
                     /pseudo
     CDS             complement(34917..35744)
                     /locus_tag="JNY02_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         9072 a         8462 c         9141 g         9069 t
ORIGIN      
        1 cagtacacct cagcgagagt gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 cttatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gagtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaac atttcattaa catgaaaaga
     1561 aaaatgatgg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgatttt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac aatgtctgat
     2161 gtgcacgacg ggtaatttcg atatagctgt aataacgaac aacaggtaat aattttttcg
     2221 ccaggatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg
     2281 cagcgtaaat attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt
     2341 tcagggcgct ggaaacaatg gtgccgcctg tttcctgcga gtagaaaaac tcatgttcat
     2401 cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc
     2461 tgctgaggaa cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat
     2521 ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc
     2581 gcttctcgta gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg tgttcaattt
     2641 tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt
     2701 cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg
     2761 ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg
     2821 ctggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt
     2881 gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat
     2941 ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggcct ggccctgacc
     3001 actgccggaa cctccgccgc caccctgggg acgttcaatt cggtcgttct gaacgaaatg
     3061 gtcattgccc ggatgcacgc ggtggcgcag accgccacgc ccctgatgaa acattggttc
     3121 gctaatatct tccgtgggaa tggatacgga ttcgctgctg tcgacgtcag tcaccgaacg
     3181 cttattaatg gcctcggaga tcgactgttt aatttgcgct ttataacggc gtaaaaaacg
     3241 ctggcgattc accatgcttt tgtttttgcc gttcagacgc cggtcaataa accaggtcat
     3301 atgccccccg tactacattt gccaactgta tgctacgcct gtgttgccgc ttctcaggcc
     3361 cacggttcgc tatcgttgta ggcccggtag catgtgtacc gggccagggt tgttaagacg
     3421 atttacgtac gcgcaaatac cattcgcaca gtaaacgcac ctgtttacgg gtgtagcctt
     3481 tttccatcat acggtcgaca aagtcgtcgt gtttcttctg ctcgtcggtt gacgttttgg
     3541 cgttaaacga gataaccggc aacagctcct cggtattgga gaacattttc ttctcgataa
     3601 ccgtgcgcag tttttcatag ctggtccagt tcggattgcg tccgctgtta ttcgctctgg
     3661 cgcgcagtac gaagttaaca atctcgttgc ggaaatcttt tggattactg atccccgccg
     3721 gtttctcgat tttctccagc tcggcgttaa gagactcgcg gtcaaacagc tgcccggtat
     3781 ccggatcgcg atactcctga tcctgaatcc agaaatccgc gtaggtaaca taacggtcga
     3841 aaatgttttg cccatattcg gaataggatt caaggtaggc cgtctggatc tctttgccga
     3901 taaactcggc atatttcggg atcagataac ctttcaggaa ctccagatag cgttctgcct
     3961 gctcttgcgg gaactgctcg cgctcgatct gctgttccag gacgtagaac agatggaccg
     4021 ggtttgccgc cacttctaca tgatcgaagt tgaacacgcg ggagaggatc ttaaacgcaa
     4081 aacgcgtcga cagaccgttc atcccttcat cgacaccggc gtagtcacga tattcctgat
     4141 acgacttggc tttcggatcg gtgtctttca gactttcgcc atcataaacc cgcatctttg
     4201 aataaatgct ggagttttct ggctctttca ggcgcgaaag aatggaaaaa cgtgacagtg
     4261 tttcgagcgt gccaggggcg catggggcgt gagtcaattc actgtgatta agcaatttct
     4321 cgtagatttt gatctcttcg gaaatgcgca agcaatacgg caccttcacg atgtaaacac
     4381 ggtcgaggaa ggcttcgttg tttttgttat tacggaaagt gacccattcg gactcgttcg
     4441 agtgggcgag agtaatcccg ttgaacggca gggcggagat accttccgtc ccgttgtagt
     4501 tatcttcctg agtggcggtt aacaagggat gcagcacttt tatcggtgct ttaaacatct
     4561 caacgaattc catgatcccc tgattggcgc ggcacagcgc accggaatag ccgtaggcgt
     4621 ccgggtcatt ctgcgcgtag tgttcgagtt tacgaatatc gactttccca accagcgcgg
     4681 agatgtcctg gttgttctca tcaccgggtt ccgttttggc gatagcaatt tgttgcagaa
     4741 ttgacggcca gaccttcact acccggaact tagtgatatc gccaccaaat tcatgcaggc
     4801 gttttgccgc ccatggcgac atgatggtgc cgagataacg gcgaggaatg ccatactctt
     4861 tttccagaat ctgagcatct tcctgcggat tgaaaagaca gaacggatga tcgttgaccg
     4921 ggctacgctc accgttggcg ctcaatacat aaatcggtac gagctgcatt aatgatttca
     4981 gtcgctcagc aagcgatgat ttacccccac ccacaggccc cagcagatac aggatttgtt
     5041 tcttctcttc cagcccctga gccgcgtgtt tcagataaga gacaatctgt tcaatcgcgt
     5101 cttccatgcc gtaaaactct tcaaatgccg gataacgtgc aatgacccgg ttagaaaaga
     5161 gtcgagaaag tctgggttcc tgggctgtat cgaccatgac aggctcaccg atagccatca
     5221 atagccgctc agccgcgttg gcataagcac tgcgatcttg ccgacaagtg gtaagaaact
     5281 cctgcagtgt gaactcttcg tccttggcag cttcatatcg ctggcgatag tgatcgaata
     5341 tattcatggt atgccgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata
     5401 gtagaggctc ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc
     5461 atgccatttt atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt
     5521 agatggcaaa tggaaaactt gcctgcacgg ctttcttaat ttcaatggca tatcaacaac
     5581 tcatccataa attttgcata attaatgtaa agaccaggct cgccagtaac gctaaatcca
     5641 tttggctgta agcgcggtgt catccgcggc aggaaaatta aacagttact ttaaaaaatg
     5701 aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca
     5761 tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat
     5821 cgcaacgtct gcaggcgttg cgcacgctga aggtaaattt tccctgggcg caggcgtagg
     5881 tgtcgttgag cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa
     5941 ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga
     6001 cgcaacggat aaactttcaa ttaccgctta ctggtcgccg ctttacttca aagcgaaaga
     6061 cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct
     6121 gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg gcgataccct
     6181 ggataacagc aacggcattg tctgggatat ggcttggttg tatcgttaca ccaacggtgg
     6241 cctgaccgtg actccgggta ttggtgtgca gtggaacagc gaaaaccaga acgaatacta
     6301 ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtgggtata acccgaatga
     6361 cagctggagc ccttaccttg agctgagcgc cagctacaac ttcctcggcg actggagtgt
     6421 ttacggtacc gcgcgctaca cccgtctgtc tgatgaagtt actgacagcc cgatggtgga
     6481 taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt
     6541 tccagggcat tttgtgcata aaaatagggc gtcacgccct gttttgcatt acggtggtgc
     6601 aatacctggg aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg
     6661 ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa
     6721 gttgctgcac tacgcgcagg cattgaactc ggtttaaccc tcattgatac cgccgaaatg
     6781 tatgccgatg gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgagagaag
     6841 gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca
     6901 tgcgaagcca gtttacgccg tctcaatact gattatctcg atctctactt attacactgg
     6961 tctggcagtt tcgcttttga agagactgtt gctgcgatgg aaaaattgat cgcccaggga
     7021 aaaatccgtc gctggggcgt ttctaacctt ggttatgccg atatgcagga actctggcag
     7081 ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg
     7141 attgagtacg atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt
     7201 ccattagtcc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa
     7261 attgcacatg tccaacaaaa tgcagctgcg cttgaggtcg aactttcgtc agcggaatta
     7321 gctatgctgg ataaggcata tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga
     7381 tgttgatacg tgccggatgc atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttg
     7441 gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt tggtgacttt ttgcgtttct
     7501 gaagcgtaag ccgtttctac acaaacaaag gtcttgtagc catcgtccgg catgtcgccc
     7561 atgctaactg aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg
     7621 cctacggcga taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgcggattc
     7681 agatacacgc gatcggtacg gtcagggaag gtctgaatac cgtcggtcag tacatcttct
     7741 ttcgcgtcat tcactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg
     7801 atatcaccca cgttaaagta ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat
     7861 tcaagatcga tttcacaggt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc
     7921 ggccagaatt ttttcgtctc ttcgctttgt gtcaattcaa aagtcagcgc tacgccatca
     7981 gcatcttcat gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc
     8041 agaccttgtt gtgccgccgg accaaaccac ggccagcaaa ccggtacgct accgcgaata
     8101 gcgacgccat ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca
     8161 ggtttccacg agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga
     8221 tcgaccacaa tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt
     8281 tcgatgaccg gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta
     8341 tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt
     8401 atttggagat gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt
     8461 accaagatac cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca
     8521 cggaagtgca aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca
     8581 gaacgccttt catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag
     8641 ttgcagcttt ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc
     8701 ggaacgccat accagtcagt ttgccattca gttctggcag tactttacct acagctttag
     8761 cagcaccggt agaggacggg atgatgttct gggaagcgcc gcggccgccg cgccagtctt
     8821 tgtgagacgg gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca
     8881 gaccttcgat gatgccgaag ttatcgttga taactttagc cagcggagcc aggcagttgg
     8941 tggtgcagga agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt
     9001 taacgaacat cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag
     9061 cggtgatgtg tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa
     9121 cgtcaacacc cacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga
     9181 tttttttacc gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc
     9241 cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga
     9301 ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac
     9361 ggccaaaacc gttgatacct actttgatag tcatatattc caccagctat ttgttagtga
     9421 ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa
     9481 tcattgcgac aaatcaatcc tgtgcctaag cattacgcgg ctgactcgcc tcactcttcc
     9541 tttgggcttg agaccacatg gggtcggcgc cccgaatttt aaagggcaat tacgataaaa
     9601 atgtgatttt catcacgatt taggtgcagc catttcgtcg agatcaagtt taggacaaaa
     9661 ttttgccagt tggtgttaat gttttgttag aatcagtcac gtaatgtgag cacgattaaa
     9721 gtgagatgtg agtaaatggc gaataaacct tcggcagaag aactgaaaaa aaatttgtcc
     9781 gagatgcagt tttacgtgac gcagaatcat gggacagaac cgccatttac gggtcgttta
     9841 ctgcataaca agcgtgacgg cgtatatcac tgtttgatct gcgatgcccc gctgtttcat
     9901 tccgaaacca agtatgattc cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa
     9961 gaatccattc gttatatcaa agacttgtca catggaatgc agcgcataga aattcgttgc
    10021 ggtaactgtg atgcccatct ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa
    10081 cgttattgtg ttaactctgc ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc
    10141 aacggttgaa aaaacgattc agcaacttat tccacaggag cggaaaaatt atgaatcttg
    10201 atgacattat caacagcatg acgcctgagg tataccagcg tttgtcgacc gccgttgaac
    10261 tggggaaatg gcctgatggc gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac
    10321 tggtgatgct gtggcaagcc cgccataata ccgaagcaca gcatatgaca attgacacaa
    10381 atggtcagat ggtcatgaag agcaaacagc agttaaaaga agattttggt atctcagcaa
    10441 agcctattgc catgtttaag taagtggttc atcccctttt tgccgcggct atgaagggga
    10501 tagccaacaa tgaccccacg actcgtctca attatctcga cccgacctgg ctaagtccca
    10561 gtccgatctc ttgcagtcgc gttcacgcct gaatgacttc tcctgaactt aagtctcaga
    10621 cctatttggc cggtaatccc tctcgaattt gctctttttg cgtcccgcct tccaccaacc
    10681 tgtaacagac ccagccattt tctgcgtaat tgtgatctaa gttgtaaaat gcaggtcatt
    10741 atctgtgttg tgcagattta atgttgacct caaaaatcat gtgggtaagt atgatggcca
    10801 tctaagcaaa acaaaacaca gaggatgacg caatgaaagc actggctcgg tttggcaagg
    10861 cctttggcgg ctacaagatg attcatgtcc cacaacccat ttgtggcccg gaagacgtag
    10921 tgattgaaat taaagccgcg gcaatctgcg gcgcagacat gaagcactac aatgtcgata
    10981 gcggttctga tgagtttaac tctatccgcg gccatgagtt cgcaggttgt attgcgcagg
    11041 ttggtgaaaa agtcaaagac tggaaagtgg ggcaacgcgt cgtgtcggat aacagcggtc
    11101 acgtttgcgg cgtttgtccg gcctgtgaac agggtgattt tctgtgttgt acagaaaaag
    11161 taaaccttgg tctggataac aatacctggg gcggtggttt ttccaaatat tgtctggttc
    11221 ctggtgaaat tctcaaaatt catcgtcatg cgttgtggga aatccctgat ggtgttgatt
    11281 atgaggacgc agccgtactt gaccctatct gtaatgccta taaatccatc gcgcagcaat
    11341 cgaaattcct tcctggtcag gatgttgtcg tcattggcac tggcccactc gggctgtttt
    11401 ccgtacaaat ggcgcggatt atgggggcgg taaatatcgt cgtcgttggt ctgcaagaag
    11461 atgtggcggt ccgcttcccg gttgcaaaag aactgggggc gacggcagta gtaaatggtt
    11521 ctaccgaaga tgtggtggcg cgctgccagc aaatttgtgg caaagataat ctgggactgg
    11581 tgattgaatg ctccggtgcc aatatcgcac tgaaacaagc catcgaaatg ctccgcccga
    11641 atggggaagt ggtacgcgtt ggaatgggct tcaaacctct tgatttctcg attaatgaca
    11701 ttaccgcctg gaataaaagc atcattgggc atatggccta tgactccacc tcatggcgta
    11761 acgctatccg gctattagcc agcggcgcta tcaaagtcaa accgatgatc acgcatcgta
    11821 tcggcctgtc gcaatggcgc gaagggtttg atgcgatggt cgataaaacc gcaatcaaag
    11881 tgatcatgac ttacgacttt gatgaataac tactctcctg aaggaacgtt atcgcatgga
    11941 acagataaca aaaccgcatt gtggtgtacg gctggatcgc ttaccggatt gccgctggca
    12001 ttcgtcaatg tttgccatcg tcgcgtttgg cttgcttgtc tgctggagta atgccgttgg
    12061 tggcttgatc ctcgcgcagc tgaaagcgtt gggctggaca gataattcca ccacagccac
    12121 attctcagca atcacgaccg ccggaatgtt tctcggtgcg ctggtgggcg gcatcattgg
    12181 cgacaaaacc ggtcgcagaa atgcgttcat cctctatgag gccattcata ttgcctcgat
    12241 ggttgtcggt gctttctcac cgaatatgga ttttcttatt gcctgccgtt ttgtgatgga
    12301 cgttgggctg ggagctttac tggttacact gtttgctggt ttcaccgaat atatgcccgg
    12361 tagaaatcgc ggaacgtggt ccagtcgggt ttcctttatt ggcaactggt catatccgct
    12421 ctgctcactg attgcgatgg gactcacgcc gctgattagt gcagagtgga actggcgggt
    12481 acaactgctt attcctgcaa tattgtcgct tatcgctacg gcgctggcct ggcgctactt
    12541 tcctgaatcc ccgcgctggc tggaatcgtg cggacggtat caggaagccg agaaagtcat
    12601 gcggagtata gaagaaggcg tcatacgcca gacgggtaaa cctttgccgc ctgtggttat
    12661 tgctgatgac ggtaaagcgc cacaagcggt gccgtattca gccttactga caggagtatt
    12721 actgaaacgc gtgatattag gctcttgtgt gctgattacc atgaacgttg tgcagtacac
    12781 actaattaac tggttgccaa caatattcat gacccagggg attaatttaa aagactcgat
    12841 tgttttaaat accatgagta tgtttggcgc gccatttggt atttttattg ccatgctggt
    12901 gatggataaa attccgcgta aaacaatggg tgtggggcta ttaatcctga ttgcggtgct
    12961 cggatatatc taccagtatg ttgctcataa cgctgattgg tttcttcctg attactttcg
    13021 tctatatgta cgtttgctat gcctcggcag tgtatgtccc tgaaatctgg ccgacagagg
    13081 ccaaactccg tggctccggt ctggcgaatg cggtagggcg aatcagtggt attgccgcac
    13141 cttatgccgt tgcagtgctg ctcagtagtt atggtgtaac gggagtcttt attcttctgg
    13201 gggctgtttc aattattgtc gcaattgcta tcgccaccat tggaattgaa accaaaggtg
    13261 tctccgttga aagtttaagt attgatgcag tagccaataa ataatacggg tggattcaaa
    13321 atgaaaaatt caaaagcaat attgcaggtg ccgggcacaa tgaaaattat ttcagcagaa
    13381 ataccagtgc ctaaagaaga tgaagttttg attaaagtag aatatgtcgg tatttgtggt
    13441 tcagatgtac atggttttga atcaggcccg tttattccgc ctaaagaccc aaatcaagaa
    13501 attggcctgg gtcatgaatg cgccgggacg gttgtggctg tgggaagccg cgtgcgcaaa
    13561 tttaaaccgg gggatcgggt aaatatcgaa cctggcgttc cttgcggtca ctgtcgttac
    13621 tgtctggaag gcaaatataa catctgcccg gacgttgatt ttatggcgac acaacccaac
    13681 taccgcggcg cattaacgca ctatctgtgt catccggaga gctttactta caaactgccc
    13741 gacaatatgg acacgatgga aggggcgctg gtggagcctg ccgcagtcgg gatgcatgcc
    13801 gcgatgctgg cagatgttaa accgggtaag aagataatta ttctgggagc aggttgtatt
    13861 ggtttgatga cgttgcaagc gtgcaaatgc ctgggcgcaa cggaaattgc cgtcgttgat
    13921 gtactggaaa aacgtctggc aatgggggaa cagcttggtg cgacagtggt tattaatggc
    13981 gcaaaagagg acactattgc acgctgtcag caatttaccg aagacatggg cgcagatatt
    14041 gttttcgaaa cagcgggttc tgcggtcacc gttaaacagg cacctttaat ctggtaatgc
    14101 gcggcggtaa aattatgatt gttggtactg tacccggcga ttcggcaatc aatttcctca
    14161 aaatcaatcg cgaagtcact atccagacgg tattccgcta tgccaatcgt tatccggtca
    14221 cgattgaagc tatttcttca gggcgattcg atgtgaaatc gatggtgacg catatttacg
    14281 attatcggga tgtacaacag gcatttgaag agtcagttaa caacaaacgc gacattatta
    14341 aaggcgttat taaaattagc gattaattaa tacgggagaa cagaacatgc tcgcagatat
    14401 cagatattgg gaaaacgatg ccacaaataa acattatgca attgcccatt ttaacgtatg
    14461 gaatgcagaa atgttgatgg gcgttaaaga cgcagccgaa gaagcgaaat ccccggttat
    14521 tatttctttt ggtacaggtt ttgtcggtaa cacctcattt gaagatttct ctcacatgat
    14581 ggtatcaatg gcacaaaaag caacggtgcc ggtaataact cattgggatc atggtcggag
    14641 tatggagatt attcataacg cctggactca tggtatgaat tcattaatgc gtgatgcttc
    14701 tgcatttgat ttcgaagaaa atattcgttt aaccaaagag gctgttgatt tctttcatcc
    14761 gctgggtatt ccggtagagg cggaattagg gcatgtcggt aatgaaaccg tttatgagga
    14821 agcgctggcg ggctatcact ataccgatcc tgaccaggcg gctgaatttg ttgaacgtac
    14881 gggctgtgac tcattggctg tcgccatcgg caaccagcat ggggtttata cgtcagagcc
    14941 gcaattgaac tttgaggtcg tcaaacgcgt acgcgatgct gtttctgttc cgctggtttt
    15001 gcatggcgca tcggggatca gtgatgccga cattaaaact gcaatttcgt tgggtattgc
    15061 gaaaatcaac attcatacgg agctctgtca ggccgcaatg gtcgcagtga aagaaaacca
    15121 ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa gctgtaaaag aacgggcatt
    15181 ggaaaaaatt gaactgttcg gttctgatgg caaagcggaa taatgatatg gataatctcg
    15241 acgttatttg tataggtgcc gctattgttg atattccatt acaaccggtc agtaaaaata
    15301 tctttgatgt agattcttat cctcttgaaa gaatcgcaat gagcaccggg gggcgatgca
    15361 ataaatgaag caacaattat ttctcgtctg gggcatcgta ccgcattaat gagccgcatt
    15421 ggtaaagatg cctcagggca atttattctc ggccattgtc gcaaagagaa tattgatatt
    15481 caaagcctga aacaggacgt cagcatagat acatctatta acgttggact ggtgacggag
    15541 gatggcgagc ggacgtttgt cacgaaccgt aacggcagtt tgtggaaatt aaatattgac
    15601 gatgttgatt ttgcgcggtt tcctcaggcg aaattattat cactggccag tattttcaac
    15661 agtcctctat tggatggtaa agcgctaaca gaaattttta cgcaagcgaa agcccggcag
    15721 atgatcattt gtgccgatat gatcaaaccg cggttgaatg aaacgctgga tgatatttgc
    15781 gaagcattga gttatgtcga ttatctgttt ccaaattttg ccgaggcaaa attactcaca
    15841 gggaaagaga cactggatga aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg
    15901 gtgattaaaa cgggtaaaga cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg
    15961 ccggcggtcg caggaataac cgccatcgac accattggcg cgggcgataa ctttgcttca
    16021 ggttttattg cggcactgtt agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat
    16081 gcaacggcgg ctatctcggt tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag
    16141 ctggtcgaac aattgctgga agaatacgaa ggataatgaa ggcaaatgaa aaagatacct
    16201 ttaggcacaa cggatataac gctttcgcga atggggttgg ggacatgggc cattggcggc
    16261 ggtcctgcat ggaatggcga tctcgatcgg caaatatgta ttgatacgat tcttgaagcc
    16321 catcgttgtg gcattaatct gattgatcct gcgctaggat ataactttgg caatagtgaa
    16381 gttatcgtcg gtcaggcgtt aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa
    16441 tgcggcattg tctgggaacg aaaaggaagt ttattcaaca aagttggcga tcggcagttg
    16501 tataaaaacc tttccccgga atctatccgc gaagaggtag aagccagctt gcaacgtctg
    16561 ggtattgatt acatcgatat ctatatgacg cactggcagt cggtgccgcc attttttacg
    16621 ccgatagctg aaactgtcgc agtgcttaat gagttaaagg ctgaagggaa aattcgcgct
    16681 ataggcgctg ctaacgtcga tgctgaccat atccgcgagt atctgcaata tggtgaactg
    16741 gatattattc aggcgaaata cagtattctc gaccgggcaa tggaaaacga actgctgccg
    16801 ctatgtcgtg ataatggcat tgtggttcag gtttattccc cgctagagca gggattgttg
    16861 accggcacca tcactcgtga ttacgttccg ggcggcgctc gggcaaataa agtctggttc
    16921 cagcgtgaaa acatgctgaa agtgattgat atgcttgaac agtggcagcc actttgtgct
    16981 cgttatcagt gcacaattcc cactctggca ctggcgtgga tattaaaaca gagtgattta
    17041 atctccattc ttagtggggc tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg
    17101 aatatcaact tatcggatgc agacgcaaca ttgatgaggg aaatggcaga ggccctggag
    17161 cgttaaatac tcaacacgat tcacctctgt ttctgtgaaa actgtgatga aaatctgtga
    17221 ttcaaatagg ttattttgaa agtaaacatc ggttatgcga taatcgcgct aatgtgatgt
    17281 aaaaagatac aggggtgtaa tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat
    17341 ggttgccaac gataaaaagg tgacagtctc aaatttgagt gggatttttc aggtaaccga
    17401 agaaaccatt cgccgcgatc ttgagaagct ggaagatgaa ggctttttga ccagaaccta
    17461 tggtggtgct gttttaaata cagcggtgtt gacggagaat atccattttt ataagcgcgc
    17521 ttcatcgttt tatgaagaga agcagcttat tgcacgcaag gcactaccct ttatcgacaa
    17581 taaaaccacc atggcagccg attccagtag taccgtaatg gaattgctca aattgttaca
    17641 ggaccgtagt gacctgacgt tgctaaccaa ctcggcggaa gcgattcatg ttctggctca
    17701 gtcagaaatt aaagtcgttt caacgggtgg ggaactaaac aaaaatacac tttccctgca
    17761 aggaagaatt actaaagaga tcatcagccg ctaccatgtc gatatcatgg taatgagttg
    17821 taaaggtctt gatattaaca gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa
    17881 aaagacaatg atccgtcagg cgacagaagt tgcgttattg gttgatcact ctaagtttga
    17941 tcgtaaagct tttgtccagt tagctgactt tagtcatatt aattatataa taactgataa
    18001 atcaccgggt gcagagtgga tagcattttg caaagacaat aatatccagc tggtgtggta
    18061 atttaagcac acgttgtcaa tattatttgt tcctgcgcct ggaaataaaa tgatgataaa
    18121 gatgcggcaa gtgaatttgc cgtaattgct accttttata gcaaatccga ggcacccaat
    18181 ggaacaatat gatcaaattg gcgcaagact ggaccgcttg cctttggccc ggtttcatta
    18241 tcgtatattt ggtataataa gctttagtct gttattaacg gggtttttga gttactcagg
    18301 taatgtcgtc ttagcaaagc tggtaagcaa tggatggtca aataatttcc tcaatgccgc
    18361 ctttacctcg gcattaatgt ttggttattt catcggctca cttactggtg ggtttattgg
    18421 tgactacttt gggcggcgca gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc
    18481 aacaggggcc gcttttgtcc ctgatatgta ctggctcatt ttcttgcgct tcctgatggg
    18541 aacaggaatg ggggcgctga ttatggttgg ctatgcctca tttacggagt ttatccccgc
    18601 gacggtgcgt ggaaaatggt ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct
    18661 gtctgcggcg ataggcgtgg tggttatcgc tttttttagt tggcgaataa tgtttcttct
    18721 gggtggtatt ggcatactgt tagcctggtt tctctcaggt aaatacttta tcgagtcgcc
    18781 acgatggctg gcagggaaag ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga
    18841 gcagcaaatt gaaagggaga agagtattcg tttaccccag cttactttga accagagcaa
    18901 cagcaaggtt aaggtaatca agggtacctt caggctcctg tttaaagggg aaatgttacg
    18961 acgtacatta gttgcgataa ctgttttaat tgcaatgaac atttcgcttt ataccattac
    19021 cgtatggata ccgaccatat ttgttaactc cggcattgat gtcgataaat caatattaat
    19081 gaccgctgtt attatgattg gtgctccggt aggaatattt attgcggcat taattattga
    19141 tcattttcct cgtcgattat ttggctccgc cttacttatt attattgccg tgttaggcta
    19201 tatctattca agtcagacta cagagtgggc gattttaatc tatggtctgg tgatgatctt
    19261 ctttttatac atgtatgttt gcttcgcgtc ggcggtttat atcccggagc tttggccaac
    19321 gcatttacgc ctgcgcggtt cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt
    19381 cacgccctat ggcgttgcgg cattattaac acattatggg tcgatcacgg tgtttatggt
    19441 gcttggtgtc atgttagtgc tctgtgcgct ggttctctcc atttttggca tcgaaacgcg
    19501 gaaggtgtcg ctggaagaga tttctgaggt gaattaaatc tttcgttgat ggcatcaatt
    19561 gccagatgta ccactgacgt gttttatttg gcctacaatg ccagtacaga attgtaggcc
    19621 agcgtaaaat tacccctgcg tctcttccca gtctgccagc gtatatagcg ttgccccagc
    19681 tgctgacatc tccataaacg cgtgtgcact gtcctggggc tggatattca cgccacgaca
    19741 gccatcggta atcacgttta ccttataacc taactgtaac gcgtccagca cggtaaactt
    19801 cacgcaatag tcagtagcca ggcccataac gatcaattca tcgatttcat gatcgcgcaa
    19861 ccagtcatca agcgcggttt tctgccgacg tccgttatca aaaaaggcac tgtaactgtc
    19921 aactaaagga ttttcgcctt tatggaacac cgctgcgatc tctttttgat tcagtaacgg
    19981 atgtaattgt gcgccttcac tgttctgcac acagtgttca ggccagaaag tttgccgcag
    20041 gccgtcgagt tggcctggcg tataaggctc tacgccgtgc tggctggcaa agctgccgtg
    20101 attcgccgga tgccagtcct ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc
    20161 aataaggcgg ttagcaacct ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc
    20221 agcacagaaa tcattttgta aatcgaccag taacagggcg cgatggggca ttgcagtctc
    20281 cttaatcatc cggcgtcagt tcgccgcgca ggttttggct catggccttg cgaatggttt
    20341 cagtatccag ttcctggctc agcaggtaat gcagtttggt tagcgtggct tctacagtca
    20401 tatctgcacc gccaataacg ccggcatggg cgaggacgtt accggtggca taaccactca
    20461 tattcacttt accggacata cattgtgtca ggttgaccac cacaataccg cgatcgctgg
    20521 cttcttgtaa ttcctgcagg aaggctttgt tttgtggcgc attacccacg ccataggagc
    20581 gcagaatcaa tgctttcacc ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc
    20641 ctggataaat cgtcactacg ccaattggtt gtggcgtgat tggatgaacg attaattccc
    20701 cttcaccgtg cggggcgggt ggcgtattca aacgacgaat atggatacca gcttccagta
    20761 acggaggaag gtttggagag gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc
    20821 ggttgccgcg atacaatcgg ttattgaaaa agagcgttac ttcgttgatc ggataattcg
    20881 ccgcaacgta caacgcattc agcagattaa tttgtccgtc agagcgtaac tcagccagcg
    20941 ggatttgtga ccctgtcaca atgaccggtt taccgagatt ctcgagcatg aacgacagcg
    21001 cagaggcggt atacgccatc gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt
    21061 catagtgcgc tttaatatct tcagcaatat gctgccagtc ttccggcgtc atatcagaag
    21121 aatccatcaa cggcgtatat tcatgaatgg tgaaatctgg catctccagg cgatggaatt
    21181 ccggcatcag cgccagttgg cgttgtagat gacctgacac cggaatataa ccctgctcgg
    21241 aacgctgcat cccgatggtc ccgcccgtgt aggcgacgta aattgatttc ttttgcatga
    21301 tattgatatg aatgatatcg aaagagcaaa agtatatacg tgaggcagga ggagcgcacc
    21361 tgatactatg cggataagga aatatggggc gagacagcgg gaagaggatt cgtcgaggta
    21421 aaaaataatg tggccgacga tctgtcggcc actcagtaca agacttaacg cacgttggcg
    21481 caggtcaggc aaaacgcata acggttttgt gggtcgttaa acgcggccag cttgtcgctt
    21541 tcacttttaa cggtagaggc taccgaggca agcggtgcag gtaacatggc ctggaacgca
    21601 tctggcaaca ttgcccggac agaaccagac atgttgtcca tcactttgtc gaagaaggtc
    21661 ggttcatcaa cgtagtattc cagatgccac tgtttcactt ttgcgagctc tgctgctttg
    21721 gcaaccgcat catcgaaatc cccgagacta tcgaccagcc cgttagcttt tgcatcctga
    21781 ccggtccaga cgtggccctg ggcgatttta tcaatttgct ccggcgtcga atgacgcgca
    21841 tcagcaacca gcgtgataaa gcgtttatag ccattctcaa tgcttaattg catcatctgc
    21901 tgcgcttccg gcggcagtgc cctggtgata gaaacatccg ccagcggtga agttgagaca
    21961 ccatcagtat gaacaccaat cgaatccaga ctattttcta cggtggtgat cacgccgaag
    22021 ataccgatag aaccggtcag ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa
    22081 atccagtaac caccagatgc cgccatgccg cccatcgata caaccacaga cttacccgct
    22141 gcccgggctg ctgccagttc agcgcgaatc acttcagacg cggtaacgct gccgcctggg
    22201 ctattaacac gcaggacaat cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt
    22261 gccgcagtgg tatcaccgcc aacattcccc tgagtttcct cgccatccat aattgcgcca
    22321 ttagcgaaga cgacaccgat gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg
    22381 taataactga tggcgcgata atttttatca gtcttactcc agccgaactc tttggtcaga
    22441 gctttttcga tttcggcact cgatgccagt gcatcgacca gcttgttttc cagtgcatat
    22501 ttcgcggtat cgccaccggt tttggttaaa ccctcaagca acccttgcgc gccagggaat
    22561 acctgctgag cagggatctg ccggttagcg gcaacagtat tcagatagtt ttgccacagc
    22621 tcaccaatcc agcggctgtc agcttcgcgg gctgccggtg acatatcatc acgaataaac
    22681 ggttcaacgg cagatttata cgtacccacg cggaacacat gggtggaaac tttcagctta
    22741 tccagcaacg atttgtagta cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct
    22801 tgcggtgaca gccaaatttt attggcgaaa ctggcgagat aatattgccc ctggctgtag
    22861 ttctcgccaa cggcataaac cggtttcccg ctgtcacgaa actctttcag ggctttgccg
    22921 atgtactgca tagacggctg gtcgccgcct gcgaagtttt tcagatccat cacgataccg
    22981 gtgatattgc ggtcgtcctt cgcctggcga atggtgttga cgatatcaaa cagtgagttt
    23041 tcctgcagac gatcggaact ggcaccaagc agctggcggc ttaatttact aaaccgctga
    23101 gaactgtctg gtttatcgac gatcacacca gaaatgtcca gcagcagtgc gccacgactg
    23161 gccgtttctt tcgaatcacc accactgacc tgcatccaga tccccacacc aaccagtacg
    23221 aggaaaataa agaacaggtt aagtaccatt tcacggacga aattcagcag acgccacgtc
    23281 catttaaaaa atccggcaat aaatcgccaa agggttcgca tgtattctcc caacttaagg
    23341 tcacacctgt cacaggcgca atagatgtgg ttatcgtaat gacccaaccg gcacttgtca
    23401 gcaggaattg cggactgtgc tgtaacaaaa tccactctcg tgttaatttt gtgaataaat
    23461 atcacgacag gagttaatca aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc
    23521 tcccgcttgg ctgaacccgc gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt
    23581 atgcgtgcgc cggaccataa gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa
    23641 gggtgcgagc gtttcagcgc cgtactggaa cagggggcga ttgctgccgg tagtgatgat
    23701 aaagctatcg acaaagcccg taatgcgccg ttccgcgcac cgctcatcat cacggtggtg
    23761 gcgaaatgca aagaaaatca taaagtcccg cgatgggaac aggaaatgtc tgccggatgc
    23821 gcggtcatgg cgatgcaaat ggcagccgtt gcccagggat ttggcggcat ctggcgcagt
    23881 ggcgcattaa ctgaaagtcc ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa
    23941 attgtcggtt ttctctacct cggcacgccg cagttgaaag catctacgtc gattaacgtc
    24001 ccggacccga cgccgtttgt aacttatttc tgataattgt cgcgaaactg tctggattgt
    24061 gagcaaaggc gcaggaattc agacactctc acttatcact tcacggaatg agggttacca
    24121 tacccggatt gcattgacag gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat
    24181 acagccacgg agctggttgc ggctgtaaaa tttccccaaa agtgttggaa accatcctgc
    24241 acagtgagca ggcgaagttt gttgatccga atttgcttgt gggtaatgaa acccgcgacg
    24301 atgcggcggt gtacgatctg ggcaatggca ccagcgttat cagtaccacc gacttcttta
    24361 tgccgatcgt tgataatcct ttcgattttg gccgcattgc ggcgactaac gccatcagcg
    24421 atatcttcgc gatggggggc aaaccgatta tggcgattgc gatcctcggc tggccgatta
    24481 acaaactttc cccggaaatt gcccgcgaag taaccgaagg tggacgctat gcatgccgtc
    24541 aggcgggtat tgcgctggct ggcggtcact ccatcgatgc gccggagccg atttttggtc
    24601 tggcggtaac ggggatcgta ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg
    24661 gatgcaaact gttcctgacg aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa
    24721 aatcactgtt gaaaccagaa catcagggac tggcgacgga agtgatgtgc cggatgaaca
    24781 tcgcaggcgc gtcctttgcc aacatcgaag gcgtaaaagc gatgaccgac gttacgggct
    24841 ttggtctgct gggccacttg agcaaaatgt gtcagggggc tggtgtgcag gcacgcgtcg
    24901 actatgaagc gatcccgaaa ctccccggtg ttgaagagta cattaagttg ggcgcagtac
    24961 ctggcggcac tgaacgtaac tttgccagct acggtcatct gatgggtgaa atgccgcgtg
    25021 aagtgcgcga tctgctgtgt gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc
    25081 cggaagcaga aaatgaggtt aaaactacag ccgccgagtt tggcattgaa ctgacggcga
    25141 ttggcgaact ggtgccagcg cgcggcggtc gtgccatggt tgagatccgt taattcaatg
    25201 cggttgttta ttgccgaaaa accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa
    25261 ccgcaccgga aaggcgatgg ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt
    25321 atcggtcacc tgcttgagca ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg
    25381 aatcttgcgg atttgccgat tgtcccggaa aagtggcaat tacagccacg accctccgtg
    25441 accaaacaac ttaacgtcat caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc
    25501 ggggacccgg atcgtgaagg gcaattgctg gtggatgaag tgctggacta tctgcaactg
    25561 gcaccggaaa agcgccagca ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg
    25621 gttgagcggg cgatcgaccg tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct
    25681 gcgctggcgc gagcgcgtgc cgactggctg tacggcatca atatgacccg tgcgtatacc
    25741 attctcggtc gcaatgccgg ttatcagggc gtactttccg tgggacgcgt gcagacaccc
    25801 gtgctcgggc tggtggtgcg ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc
    25861 tttgaagtca aagcacatat cgtgacacct gccgatgagc ggtttaccgc tatctggcaa
    25921 ccgagcgaag cgtgtgaacc gtaccaggat gaagaagggc gcttgttaca tcgtccactg
    25981 gcggagcatg tggttaaccg cattagtggt caaccggcta ttgtcaccag ctataacgat
    26041 aaacgggaat cagaatccgc gccgctgccg ttttcgcttt cggcgttgca gattgaagcg
    26101 gcaaaacgct tcggtctgag cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa
    26161 acgcacaagc taatcactta tccgcgttcc gattgtcgct atttgccaga agaacatttt
    26221 gccggacgcc acgcggtgat gaatgccatc agcgttcatg caccggatct gttgccgcag
    26281 ccagtggtag atccagatat acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac
    26341 cacgccatca tcccgaccgc acggagttct gcgatcaacc tgacggagaa cgaagcgaag
    26401 gtctataacc tgattgcccg tcaatacctg atgcagttct gcccggatgc ggtgttccgc
    26461 aagtgtgtta tcgaacagga cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt
    26521 gctgaagcag gctggcgcac gctgttaggc agcaaagagc gcgatgaaga aaacgacggc
    26581 acgccattgc ctgtggtggc gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta
    26641 gagcggcaaa cccagccgcc gcgccatttt accgatgcaa cactgctttc ggcgatgacc
    26701 gggatcgcgc gctttgtgca ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt
    26761 ctggggacag aagcaacccg tgccgggatt attgaactgc tgttcaagcg tggttttctg
    26821 accaaaaaag ggcgctatat ccactccacc gacgccggaa aagcgttatt ccattcgctg
    26881 ccagaaatgg cgacgcgacc ggacatgacc gcgcactggg aatcggtgct gacgcaaatc
    26941 agcgaaaagc agtgtcgcta tcaggacttt atgcagccgc tggtggggac gctatatcag
    27001 cttattgatc aagccaaacg tacgccggtg cggcagtttc gcggcattgt ggctccgggc
    27061 agtggtggca gtgctgataa gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt
    27121 ccgccagcag atgaagccgg aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta
    27181 ttcgagtcat attctgaaat atccagcgga tcaagaaaat tcgttggata tttttttgca
    27241 tggataaaat tatcgcctct aaagtatgta ataacaggga atgtgatgaa aaaagtttta
    27301 ttacaaaacc atcctgggag cgagaagtat tcttttaatg gctggggaat atttaatagt
    27361 aattttgaac gggtgattaa agaaaataag gccatgctgc tttgtaagtg ggggttttat
    27421 ttaacatgtg ttgtcgctgt aatgtttgta ttcgcagcga taacatccaa cggtttgaat
    27481 gaaagaggcc tgattaccgc gggatgctct tttctttatc tattaattat gatgaggctt
    27541 attgttcggg ccggttttaa agcaaaaaaa gaacaactac attattatca ggctaaaggt
    27601 attgagccgc tcagtatcga gaagttacag gcgctacaat tgatcgcacc ttatcgattc
    27661 tatcataagc aatggtctga aacgctagag ttctggccgc gaaagcctga acctggcaaa
    27721 gataccttcc aatatcatgt gcttcctttt gactcgatcg atatcatcag taaaagacgc
    27781 gagtctttag agaatcaatg gggtatcgaa gatagcgaaa gttattgtgc cttaatggag
    27841 cattttcttt ctggcgacca tggagccaat acctttaaag caaacatgga ggaagcccca
    27901 gagcaggtta tcgccgtgtt gaataaattt gctgtttttc cctcagacta tatctctgat
    27961 tgcgctaata atcgctccgg taaatcctcg gcgaagctaa tatgggcggc ggaattatca
    28021 tggatgatct cgatatcaag cacagctttt caaaacggga caattgaaga agaactggcc
    28081 tggcattata taatgcttgc ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat
    28141 tatcaaaaaa atagtctaat gggatttctt tactggcata tctgctgcta tcgcagaaag
    28201 ttaacggatg ctgaacttga agcgtgttat cgttacgaca agcagttttg ggagcactac
    28261 agtaagaaat gccgttggcc cataagaaat gttccctggg gagcatcatc cgttaaatac
    28321 tcataaacgc ctgaaatttt gccgggggcg ctgcgcttgc ccggcctaca ttagagcgta
    28381 actgcaatga attgtgccca tctgtaggcc tgataagcgt agcgccatca ggcatttaca
    28441 acttaaatca caccctgcgc cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc
    28501 gcgccctgca cgtagttggt ttgctcacct tcaccaccat gctcaacaca ggcatggtgg
    28561 atatccagca tgatgtgatg caaacgtgcg tcaactttct cggctttcca gcccaggcgc
    28621 gcagcgtttt gtgccatttc caggcccgac gttgctacac cgccagcatt agccgcttta
    28681 cccggtgcaa acagtacgcc tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc
    28741 atatttgccc cttcagcgac ggctttaacg ccattggcga taagctgatg cgcggtgtca
    28801 acatccagtt cgttctgggt ggcgcaaggc agggcgatat caaccggtac agaccacggc
    28861 tgttggcctt cgagatagac cagaccaaat tctttggcgt aatctgccac tcgaccatcg
    28921 cggctggctt tgatttcgat aagacgtgcc agtttctctt tcgtgaatcc gctttcatca
    28981 actacagtgc cgctggagtc tgacgcagtg atcgcacgag caccaaattc catcgctttt
    29041 tcgatagcgt actgggcgac gttgccggag ccagaaacgg aaacgcgcat cccttcaaaa
    29101 cccataccgt ggcgttttaa cattgcttct gtgaaataaa ccagaccgta gccggtagct
    29161 tccgggcgaa taagactacc gccaaatgaa aggcccttac cggtgaagac gcaggcggta
    29221 ttgttggaga gctttttcat catccccgcc ataaagccga cttcacgacc accaacgccg
    29281 atatcacctg ccggaacgtc ggtatccgcg cccaggtggc gatacagttc agtcatcagc
    29341 gcctggcaaa aacgcatcac tttaccttcg ctttttcctt tcggatcgaa atcgctgccg
    29401 cctttaccac cgcccatcgg cagggtagtc agggcatttt tgaaggtttg ttcaaagccg
    29461 aggaatttga gaatggaaag gttaactgac ggatggaagc gcataccgcc tttgtacggg
    29521 ccgatggcag agctgaactg cacacgccat gcacggttga cctgtatctg gttgcgatca
    29581 tcaacccata ccacgcgaaa ctggatcacg cgctccggtt caaccagacg ctccagtaat
    29641 gacatctggc gatattttgg attttgttca agaaaaggcc agagtgtggt cattacttca
    29701 cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct tttggacatg gttgaggaat
    29761 gactccagag aatatgtctg atccatagat ataaaaacct tatatattaa tacgattgtg
    29821 cttatgttgt ttatgtcatg tgcttttgca gttttcgact ataccatcaa gaatgtgacg
    29881 gtaagcaaga aaaaaatgac ccaggaaagc aaattaataa cgagagtaat ctcataacca
    29941 aatgcgataa ctcagacgct aaagttttgc cacccggcta ccgttatcag tagcagagac
    30001 aataacgtta aggagttccc tgtgagtcga gcattgttcg ccgtagtatt agcatttcca
    30061 ttaattgcac tggctaaccc gcattatcgt ccggacgtgg aggtaaacgt accgccagaa
    30121 gtgttcagtt caggcgggca gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat
    30181 caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa tactgctgca atgccagcgt
    30241 gatgacaaaa cccttagcac taatccgctg ctctggcggc gcgtaaagcc ataaacgcct
    30301 ctaataatgg aatgtcagca ggggccagcg gatattgcag cgcctcttca ggtgagcacc
    30361 agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg
    30421 catgaagatg gataatccgg cccgaaactt ctcgctgatg gctggcaaca tactcaccca
    30481 cagttgcttc gatgtccaat tcttcgttta actcacgcac cagcgcctgc tgctggcttt
    30541 catccagctc gactttacct ccggcaaact cccataatcc cgcctgatcg ctctgggcgg
    30601 ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat ggcggcaaca acttcaatca
    30661 ttttcataaa ttacacacgg gcagataagc gaaaaatgcc ataatatccg cactgttgct
    30721 caatcagaaa ggctttatat ttaaggagtg cctgtgctag accgccatct tcatccccgg
    30781 attaaaccgt tgttgcatca gtgcgtgcgg gttcttgata aatcgggcat tacgccagat
    30841 ggtttaacat tagtgggatt tgccatcggc gtgctggctc tgccgtttct ggcgctgggc
    30901 tggtatctgg cggcgctagt cgtcattttg ttgaacaggc tgcttgatgg tctggacggt
    30961 gcgctggcgc ggcgcagagg gcttaccgac gcgggcggct ttcttgatat ttctctcgat
    31021 tttctctttt acgcgctggt gccgtttggc tttattcttg ctgcaccgga gcaaaatgcg
    31081 ctggcaggcg gctggttgtt gtttgcgttt atcggcacgg gcagcagttt tctcgccttt
    31141 gctgcactgg cagcaaaaca tcagattgat aaccccggtt atgcacataa gtcgttttac
    31201 tatctggggg gattaaccga aggcacagaa acgatcttac tgtttgtgct gggatgttta
    31261 ttccctgcat ggtttgcgtg gtttgcgtgg atcttcggtg cgttgtgctg gatgacgacc
    31321 tttacgcggg tgtggagtgg gtatctgacg ctgaagtcgc tccagcgtca gtaattgcaa
    31381 tgttatttgc tactgcccgg gccgcgttca ccggttgcaa ccggattttt tggatcgctg
    31441 ctccattcgt accagccgcc gtcatacacg gaaacattct tccaacccat ggcgcgtgcg
    31501 tacataaagg tttcggacgc gcgccagccg gtgccgcagt agaatgaaac ttgctgatct
    31561 ggtttgatat tccatgcttt ccacatagcg gtaatatcat cggcgctacg catggtgcca
    31621 tccgggttat ggaaatcttc catatgcgtc gagtcgctac cagcgtgtcc ccaacgtgct
    31681 ccggctattt caccttttgg tttaatatag ctgtaaccgc tggtcgtacc gataaattct
    31741 ggccacgaac gaatgctcac cagcgatgca tcctggcgat gcagcagtcc acgggcctgt
    31801 tccatatcga gcatcaactg cggttgtgcc gggatcttca cgccgaaatc tggttccgct
    31861 ttcactttcg gtggcgttcc tcgctcaaca ggcagtcccg cgtcggacta ggtttgccag
    31921 ccgccatcca gcagacgcac atctttcacg cctgcgtaga gcataatttg cgccacacgc
    31981 gctccagcgt atacgtcacg cccgtacaga atgaccgtgg tgtcatggcg aatgccgtgt
    32041 tttgccagca tcgcttttag ttgttcatca gaaactttgt tccacagcgg ttcgctttcc
    32101 acttcgttgg tatcgatgta gtcagcgcca ggaatatggc tgataaggta aagcttagga
    32161 gcgccccagg ccgcttcaat gactttccag ccaccgacag gtttcgccgt aacctcttta
    32221 ccttgttgca ggtcatgcag ccattgcgga taaaccagct gctcaaagtg cggcagtttt
    32281 tgtagacggg aaggttcgct tagcgtgtca ctcagaatgg agatatgcgt aaaacctgct
    32341 ttttgcagtc gcgttttgac ggcgtcgaca tctttgccat taccgtacag cgccaccggg
    32401 gcatcggctt tcaggttatg ttgcttgatc cacgcgttga gctgttcggt gctcattttg
    32461 tcgagccagc tagcagagag gtttaaggcg gcaggttcat gaccagaagg gccatttaag
    32521 gtttgtggcc agccgttata aaacgcgctg gggcgagtat ctatcgcttt gccattttgt
    32581 tgctgaagtt ggtcaagtgt aagaggcttc gccagttcag cggcccacga agaagcgcaa
    32641 gccagcccta aagccattgc cagcgcggtc atttgagaaa cacgtttcat cgaaaatccc
    32701 gacattatag ttttgagtgc gccattctga actgacgcat tgagaaaaac tttgcgttat
    32761 cgcagtttgt tgtaattttc tgaccactgc gccatatcca gaacagaact atcagcagga
    32821 acatcctgga gatcgtgcgt tacctgaacg acggggatcg ccagggcgcg aacttcgctg
    32881 aacacccact ggcgaaaatt atcgcgcaga gccacatcaa gacggctgaa tggctcatcc
    32941 aggagtaacg cttttggctg ggcgagaagg gcgcgtagta gagcaacgcg cgctcgctga
    33001 ccgccagaca aagtggcagg atcctgatgg aaagctccgt caaggcctga acgctcaagt
    33061 gcatcattca cggcattacg tcgggcattc cccttaagtg tcgccggtag cgccagcagt
    33121 aaattttgcc cgacgctgaa ctggtcaaat aacagtgcat cctgaaaaag aatgccaatc
    33181 tgacgctgtg cggtgggtag catgtcaatc cgttgctcat tgagccatag ctcacctgta
    33241 caagaaaact gtccggccag tgcaccaatc atccatgaaa acagagtgga ttttccacag
    33301 ccagatggcc ccattaacgt gacgatgtca cctttatcaa ccgtaaagtt aacgtttgtc
    33361 agcaagcggc tttctggtaa acgtagcgaa acatttttca cgcagagcat tagcggattc
    33421 cttgtctgac ataacctacc cattttgcga ctaacgcggt cagggcaaaa ataataagcg
    33481 gtaacaacag ttgccataaa gcctgggcgg cgagaatacc gttgctgccg ccgctgctta
    33541 atgccaccgc ctcagtggtg agcgtcggaa aacgccccgc gcccagccac agcgtcggca
    33601 tatactgcgc aatactgact gaaaatccca ccgcgaaggc aatcagcaca gggcgcaaca
    33661 tgagtgggca tttcacgtaa aagaagattt tggcccgcga ccagcccagt gtttgcgcaa
    33721 tcaaaattaa ccgtgaatca atgcgctgcc aggcgggttg caggataaac agcatccacg
    33781 gcattaccca cagcagatgc ccccagacca ccgccgtcca gcttccatcc aggttcagcc
    33841 atagcgccag cgtgtactgg cccgccacca gtggcagagc gggcaataga atgggcaacc
    33901 atagccacaa ctggcgacgc tgtgcccccc attccagcca cagcagtaac aggagcaaag
    33961 cgatgaatgt tgctaccagc cccattgtca gactgttgat gagtgcttca ctattgatcg
    34021 tcgactgatc cgcgaggatc gccagcagaa ccacacacag cacaccggtt aagggtaaaa
    34081 aactcgccag cgtagtgcct ggtaataaag gtgtggcagg cttgcgaatg ccatctacgc
    34141 ggggaatagt gcgccgccag ctgcgccata gcagatagct cagcaaaacg taggcggcga
    34201 gtaacagcat cagcagcaga ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct
    34261 gagttaacca ctgccagcta attactgcca gcgtcggtgg attacctggc ccaagaataa
    34321 tcgccacatc cacgaccgaa agcgaccagg caacaatcgc cagcatcgcc attgccagcg
    34381 aaggcgcgac ggagggcaac agcagccagt tcaggcattg ccagcggctg tagcccagcg
    34441 aatccagcac aatgacctgc tgcaacagcc gtttttcgct caatactgcc actaagatcc
    34501 acagcagaaa tgcgctttct ttcactgcaa gggtgaggcc cagaccgatc caaatcggtc
    34561 cattggcgga gtgaaatacg ggaaatagtc atatagcagc cctccgtcag caaagagcag
    34621 cagggcgctg gtggcaaaag ccacatgggg aatggcgagc agccacggca gacgggcgca
    34681 catacgctgc catttcggcc caggccatag cgccacaatc accagcaggg caatcaacaa
    34741 tgccccgacc gccacgatgg ttgtcgacac cagcgttgcc agtaatgcct gcggtaactg
    34801 tggatcggca aataacgcct gccagcgtgt caacgacaac gcaggcgata tcagcgtgag
    34861 agctgccggg atcagcggtg cataaatcac cgccaccatc gcccacagca aaaagattaa
    34921 tgcgtaccgt aacggcgtaa ccattcttgt tccagtgcat tcacccatcc tgcgtgcggt
    34981 tcagccagta ccggcggcag atcctgcggc attcttgatt gcaatatttc gcgctgcccg
    35041 tcaggcagtt tttgcggatc gagaacagaa ggatcgcccc agacaacggg atctgcttta
    35101 cgcagttgcg catcgggtga gagcaggaaa ttggcaacta ccttcgccgc agcactggca
    35161 ttcgcgttgg ctggaatggt gacgaaatgc acgttgccaa tcatcccctc gcgaaagcca
    35221 aaactgtaac tgcttgccgg caaatcgccg ctggcgattt tttgctgcgc atgcgcgggg
    35281 ttaaaggtca gcgacaggcg caatgtgccg gctttcagca gagcatccat ccgcgcgggc
    35341 gaaggcggga aatcctttcc ttcgcgccac aaatacggat gcagtgcatc aagatattgc
    35401 cacaagggcg cagtgacacg ggcgaaagtc gcatcgtccg gcgcttcttt taatgcggcg
    35461 ggatctggcg tcagcataat cagcaactgt tcaagaaacg ccgtgccggt aaagtccggt
    35521 gggcgcggat aagtaaccgt gccgggattg gctttagcaa actccagtaa ggcttgcggc
    35581 gtttgtggtg gctgtggtaa acgtcagttg tgcgccgccc cagggcgatt ccgcaccttg
    35641 tgtcggcact gaaaaatctt cccgcaccgg cagctgtgtg tcgacatagc gccagttggg
    35701 cagagtctcc gcccagcccg tttgtaataa attggcctct ttta
//
LOCUS       DADWTH010000034        35252 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.034, whole genome shotgun sequence.
ACCESSION   DADWTH010000034 DADWTH010000000
VERSION     DADWTH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.034"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1151)
                     /gene="setB"
                     /locus_tag="JNY02_001758"
     CDS             complement(<1..1151)
                     /gene="setB"
                     /locus_tag="JNY02_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBB9433318.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1519..2649
                     /gene="fruB"
                     /locus_tag="JNY02_001759"
     CDS             1519..2649
                     /gene="fruB"
                     /locus_tag="JNY02_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBB9433319.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2649..3587
                     /gene="fruK"
                     /locus_tag="JNY02_001760"
     CDS             2649..3587
                     /gene="fruK"
                     /locus_tag="JNY02_001760"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBB9433320.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3604..5295
                     /gene="fruA"
                     /locus_tag="JNY02_001761"
     CDS             3604..5295
                     /gene="fruA"
                     /locus_tag="JNY02_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBB9433321.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5719..6660
                     /locus_tag="JNY02_001762"
     CDS             5719..6660
                     /locus_tag="JNY02_001762"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBB9433322.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6648..7586
                     /gene="psuG"
                     /locus_tag="JNY02_001763"
     CDS             6648..7586
                     /gene="psuG"
                     /locus_tag="JNY02_001763"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBB9433323.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7680..8930
                     /locus_tag="JNY02_001764"
     CDS             7680..8930
                     /locus_tag="JNY02_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBB9433324.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9002..9700)
                     /gene="yeiL"
                     /locus_tag="JNY02_001765"
     CDS             complement(9002..9700)
                     /gene="yeiL"
                     /locus_tag="JNY02_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBB9433325.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLT
                     LEMDPENKFSGMMQ"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="JNY02_001766"
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="JNY02_001766"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBB9433326.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10872..12122
                     /locus_tag="JNY02_001767"
     CDS             10872..12122
                     /locus_tag="JNY02_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBB9433327.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVIGILIRILGGIFQKALNISKIESFVAVTTIFLVQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13262)
                     /locus_tag="JNY02_001768"
                     /pseudo
     CDS             complement(12178..13262)
                     /locus_tag="JNY02_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(13265..14122)
                     /gene="nfo"
                     /locus_tag="JNY02_001769"
     CDS             complement(13265..14122)
                     /gene="nfo"
                     /locus_tag="JNY02_001769"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBB9433328.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14196..15245)
                     /locus_tag="JNY02_001770"
     CDS             complement(14196..15245)
                     /locus_tag="JNY02_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBB9433329.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15344..16225
                     /locus_tag="JNY02_001771"
     CDS             15344..16225
                     /locus_tag="JNY02_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9433330.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16431..17900
                     /gene="lysP"
                     /locus_tag="JNY02_001772"
     CDS             16431..17900
                     /gene="lysP"
                     /locus_tag="JNY02_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBB9433331.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18192..20183
                     /gene="cirA"
                     /locus_tag="JNY02_001773"
     CDS             18192..20183
                     /gene="cirA"
                     /locus_tag="JNY02_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBB9433332.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20215..21051)
                     /gene="yeiG"
                     /locus_tag="JNY02_001774"
     CDS             complement(20215..21051)
                     /gene="yeiG"
                     /locus_tag="JNY02_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBB9433333.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATYYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21309..21977
                     /gene="folE"
                     /locus_tag="JNY02_001775"
     CDS             21309..21977
                     /gene="folE"
                     /locus_tag="JNY02_001775"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBB9433334.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21994..23151
                     /locus_tag="JNY02_001776"
     CDS             21994..23151
                     /locus_tag="JNY02_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBB9433335.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23293..24332
                     /gene="galS"
                     /locus_tag="JNY02_001777"
                     /pseudo
     CDS             23293..24332
                     /gene="galS"
                     /locus_tag="JNY02_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24612..25610
                     /gene="mglB"
                     /locus_tag="JNY02_001778"
     CDS             24612..25610
                     /gene="mglB"
                     /locus_tag="JNY02_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBB9433336.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25671..27191
                     /gene="mglA"
                     /locus_tag="JNY02_001779"
     CDS             25671..27191
                     /gene="mglA"
                     /locus_tag="JNY02_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBB9433337.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27207..28217
                     /gene="mglC"
                     /locus_tag="JNY02_001780"
     CDS             27207..28217
                     /gene="mglC"
                     /locus_tag="JNY02_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBB9433338.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28253..29488)
                     /gene="preA"
                     /locus_tag="JNY02_001781"
     CDS             complement(28253..29488)
                     /gene="preA"
                     /locus_tag="JNY02_001781"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBB9433339.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29482..30721)
                     /gene="preT"
                     /locus_tag="JNY02_001782"
                     /pseudo
     CDS             complement(29482..30721)
                     /gene="preT"
                     /locus_tag="JNY02_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30915..31154)
                     /locus_tag="JNY02_001783"
     CDS             complement(30915..31154)
                     /locus_tag="JNY02_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBB9433340.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31157..31876)
                     /gene="sanA"
                     /locus_tag="JNY02_001784"
     CDS             complement(31157..31876)
                     /gene="sanA"
                     /locus_tag="JNY02_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBB9433341.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32026..32910)
                     /gene="cdd"
                     /locus_tag="JNY02_001785"
     CDS             complement(32026..32910)
                     /gene="cdd"
                     /locus_tag="JNY02_001785"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBB9433342.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33040..33735)
                     /locus_tag="JNY02_001786"
     CDS             complement(33040..33735)
                     /locus_tag="JNY02_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBB9433343.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEITASILPKSVTTPIAMSVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33732..34130)
                     /locus_tag="JNY02_001787"
     CDS             complement(33732..34130)
                     /locus_tag="JNY02_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBB9433344.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34369..>35252
                     /gene="dusC"
                     /locus_tag="JNY02_001788"
     CDS             34369..>35252
                     /gene="dusC"
                     /locus_tag="JNY02_001788"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBB9433345.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8662 a         8614 c         9306 g         8670 t
ORIGIN      
        1 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
       61 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      121 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      181 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      241 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
      301 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      361 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
      421 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      481 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      541 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      601 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      661 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      721 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      781 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
      841 ttgcgattcc aggcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
      901 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
      961 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     1021 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1081 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1141 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1201 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1261 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     1321 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1381 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1441 gtctttaacc ctaagccagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1501 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1561 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1621 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1681 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1741 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1801 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1861 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     1921 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     1981 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2041 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2101 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2161 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2221 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2281 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2341 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     2401 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2461 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2521 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2581 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2641 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2701 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2761 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2821 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     2881 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     2941 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt tgaagtcacc cccgccgact
     3001 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3061 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3121 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3181 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3241 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3301 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3361 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3421 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3481 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3541 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3601 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3661 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3721 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3781 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3841 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     3901 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     3961 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4021 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4081 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4141 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4201 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4261 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4321 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4381 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4441 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4501 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4561 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4621 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4681 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4741 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4801 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4861 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     4921 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     4981 caggaaggtg gcaaagccgc tctggtattg gggctgtgct tcatttcgga aggtgcaatt
     5041 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5101 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5161 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5221 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5281 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5341 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5401 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5461 tattttctgc ccttttttag tgttcctgtt ctgtgtatcc cgtcacaaat tcctcgcttt
     5521 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5581 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5641 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5701 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5761 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
     5821 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
     5881 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     5941 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6001 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6061 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6121 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6181 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgtggaaatg tgtcaaagtg
     6241 cgcgaccgtc taaatcagat ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6301 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6361 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6421 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6481 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6541 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6601 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
     6661 aaatttcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6721 aaccggttgt ggcgctggaa tcaaccatta tttctcacgg gatgccgttc ccacaaaatg
     6781 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6841 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     6901 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     6961 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7021 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7081 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7141 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7201 taattggtta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7261 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7321 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7381 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7441 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc ggcggtgaca
     7501 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7561 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7621 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7681 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7741 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7801 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7861 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     7921 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     7981 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8041 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8101 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8161 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8221 tggcgtccat tgctggttcg atgatgattg gttatgccgg aatgggcgta ccaattgact
     8281 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8341 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8401 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8461 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8521 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8581 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8641 gcctgattgg gcagaaactg gcgattaacg aattcatcgc ttacctgagt ttctccccat
     8701 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8761 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8821 caaaacgtgc tccagaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     8881 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctacgatt
     8941 tgtagaccgg ataaaacgta ttaagcgttg catccggcaa tcgcgctatt gcaaataatt
     9001 tttactgcat catcccggag aatttattct ccgggtccat ctccagcgtc agtcctgaca
     9061 actgctttct gtttttaatt agatatcctt tcttgctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcattaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcagca aagaaatcga
     9481 tcagcgacac gcgaccatta gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcatcagcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ccaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatagggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcgtg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacagttgc tgaacacctt gctgatgcaa tgcattcact
    12481 gcggcattac ggtcagcatc gctggtgatc gcctgtcccc ataaaatttc cagctccggt
    12541 aaagtgggtt tcagggtgtg aatatgcgcc agccagtgtt tgattttgcc cgctttgaat
    12601 tctgaaacgg tatcgacaaa caccgggatc tcatcagcga gggtaaagac ccattccagc
    12661 gcctcggctg tcaggttgca atcaaccagt accacgcccg catgacgaag taaatcgcgc
    12721 gacccgttca gtagttgcgg tgtcaactgt tccagcagat gggtatcgtt aatcgccagc
    12781 acggtttgat cgtctcgatt ggcaattgcc agatacgtcg atgtgctttg accatgcaaa
    12841 cgaacgcagc cggagacatt cacgccggca cggcgcgttt cttccaggag catttcgcca
    12901 taaaagtcat cgccaatcac tgaaagcaaa tggacgtcac ggcctaacag cgccagattg
    12961 tgggcgatat tgcgtccaac gccgcctgcc gaacaatgaa ttgtaccggg atgagaagcc
    13021 gcttgcgggt aatggatatc cgccatcccg cgaatatcca tattgattgt ccccaccact
    13081 acgcagtatt cctgctcggt gagaatgtaa cctttgcctt taatccggcc tttgcgcatt
    13141 aaatccataa tatgcgccgc aacgcgcgaa cgactgattt gcagcatgtc cgcaatttcg
    13201 ttctgctgaa tcagcgggtt acgccgtaaa attgcaagga tctccttttc ccggttattc
    13261 atcttcaggc taccgctttt tcagtttgtt gcgctttcag ccaggcgatc tcttctgccc
    13321 agatatccgg gttgatggtt tcaaggatca gcggaatgcc gtcgaaacgg tcgttctgca
    13381 taatccagcg gaacgcatca tgaccgatat tgccttcacc gaggctatga tggcggtcaa
    13441 cgcggctgcc aaaggtactt ttcgcatcgt taaggtgcat cccgcgcaga tacttaaagc
    13501 cgacaatacg ggcaaaatca gcgaacgttt tttcgcattc ggctggagta cgcaaatcat
    13561 acccggcagc gaaagcatgg caggtatcaa tgcagacgcc gacgcgggat ttatcttcca
    13621 cgccgtcgat aatcgccgcg agatgttcga atttaaaccc taagttactg ccctgaccgg
    13681 cggtgttttc aatcaccgct gttacacctt gagttttatc cagcgcaatg ttgatggatt
    13741 cggcaatacg cgcaaggcaa tcctcttctg aaatctgcat cagatggctg ccagggtgga
    13801 agttgagcaa agaaagcccc agttgttcgc aacgctgcat ttcatctata aaggcatcgc
    13861 gcgatttttc cagagcttca gtgaccggat gtccgaggtt gatcagataa ctgtcgtggg
    13921 gaagaatttg cgccgatgtg tagtgatatt tttcacaggc ggctttgaat tcatcgatgg
    13981 tttgcgtcgt gagcggtgcg gcacgccact gacgttggtt tttggtgaac aaggcaaacg
    14041 cggttgcgtc gatttcggcg gcgcgaattg cggcatttgc cagaccgcca gcagcactaa
    14101 cgtgcgctcc aatgtatttc atgcgaggac tcctgttaaa cccgctggag gaaaacggta
    14161 atgatagcgg gttaacagga gcaagaagta gtggtttatg cgatgacgct ttgaatcaca
    14221 tagttaatcg caccaccacc aacaatcagc caggcaaaca gtaccagtgc catcagcaga
    14281 ggtttcgccc cagctttttt cagcgcgctg acgtgagtgg tcagacccag cgccgccatc
    14341 gccattgcca gcaggaaggt atccagcgtt accagcatgt tcaccacgct ctgcggtaac
    14401 aggtggaacg agttaaagat ggcaactacg atgaacaaga tggcaaacca cggaatagtg
    14461 attttgcttt tctcgccgct gttcgcccca gacagctgtt taacacgcgc cgccagcagg
    14521 atgaggaacg gagccagcat catcacgcgc agcattttgg aaataactgc tgcgttttct
    14581 gcatccgggc tgatggcatg acctgccgcc accacctgcg ccacttcgtg cacagtagaa
    14641 ccaatgtaga taccgaaagt ttccggacta aaccattgag acatcagcgg atatatcgcc
    14701 gggtagagga aaatcgcgac ggtcccgaag ataacaacgg ttgcaacagc cacggttact
    14761 ttactggctt ccgctttcac taccggctca gtcgccagta ccgcggcagc accacagata
    14821 ctgctaccgg caccgatcaa ccaactggtg tgcttatcca gaccaaacac tttctgcccc
    14881 aggaagcaag ccagcaggaa ggtactggac agcgtcagca cgtcaatgat gatcccactg
    14941 ataccgacat cggcaatttg cgagaacgtc agacggaagc cataaagaat gatacccaga
    15001 cgtaataaat attgcttggc aaacagcaca ccaccgtcac agcttttcca gatgtgcgga
    15061 tagatggtgt tgcctaaaac catccccaac aagattgcga gggtgagggc actaaacccg
    15121 gcacccgcaa ccgcgggaat ggaaccaccc cacagggcga ccccggtgat aactgcactc
    15181 agggctaacc ccggaataaa atgccacagt gtacgatgtt gtttctgtaa ggtgatattc
    15241 gtcataaccc tctcctttaa ccgggcataa ggttacgact aactggttta aaaataaaat
    15301 tgattatata tttataatta atctttataa gtggtaagcg actatgcaca tcaccctccg
    15361 gcagttggaa gtttttgcag aagtattgaa aagtggatca accacccagg cgtcggtgat
    15421 gctggcgttg tcgcaatcag cagtgagcgc agccttgact gacctggaag ggcagcttgg
    15481 cgttcaactg tttgatcgcg tggggaaaag actggttgtt aatgaacacg ggcggctgct
    15541 ctatccgcga gcgttggcac tgcttgaaca ggcggttgaa atcgaacaac tgtttcgcga
    15601 agacaacggc gcgattcgta tctatgccag tagtaccatc ggtaactaca ttctgcctgc
    15661 agttatcgcc cgttatcgcc atgattatcc gcagttgccg attgaactta gcgttgggaa
    15721 cagccaggac gtgatgcaag cggtgctgga tttccgcgtt gatattggct ttattgaagg
    15781 accgtgccac agcactgaaa tcatttctga accgtggctg gaagacgagc tggtggtttt
    15841 cgccgcgccg acttcgccgt tggctcgtgg tccggtcacc ttagaacagc tggccgctgc
    15901 gccgtggatc ctgcgtgaac gcggttccgg cacgcgggag attgtcgatt atctgttgct
    15961 gtcacattta ccgaagtttg agatggcgat ggaattaggt aactccgagg caatcaaaca
    16021 tgcggtgcgt catgggttgg gaattagttg cctgtcgcga cgtgtgattg aagatcaatt
    16081 gcaggcaggc acattaagtg aagttgcggt ccctctgccg cgcctgatgc gtacgttgtg
    16141 gcgtatacat catcggcaaa aacacctttc caacgcgcta cggcgctttc tggactattg
    16201 cgatcccgca aatgtgccgc gttaagttgc tgtacaagaa catgctggtg ctgtgtcgat
    16261 ttcgtgacgc agcgccttca gcatgcattc gccagaaaag agattggctg ctttacttat
    16321 aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat ttctgccgga aggattgcca
    16381 atcgtctgct acaatcgcgc catcattttt aagatggata gcatttttgt atgggttccg
    16441 aaactaaaac tacagaagcg ccgggcttac gccgtgaatt aaaggcgcgt cacctgacga
    16501 tgattgccat tggcggttcc atcggtacag gtctttttgt tgcctctggc gcaacgattt
    16561 ctcaggcagg tccgggcggg gcattgctct cgtatatgct gattggcctg atggtttact
    16621 tcctgatgac cagtctcggt gaattggctg catatatgcc ggtttccggt tcgtttgcca
    16681 cttacggtca gaactatgtt gaagaaggct ttggcttcgc gctgggctgg aactactggt
    16741 acaactgggc ggtgactatt gccgttgacc tggttgcagc acagctggtc atgagctggt
    16801 ggttcccgga tacaccgggc tggatctgga gtgcgttgtt cctcggcgtt atcttcctgt
    16861 tgaactacat ctctgttcgc ggctttggtg aagcggaata ctggttctca ctgatcaaag
    16921 tcacgacagt tattgtcttt atcatcgttg gcgtgctgat gattatcggt atcttcaaag
    16981 gcgcgcagcc tgcgggctgg agcaactgga caatcggcga agcgccgttt gctggtggtt
    17041 ttgcggcgat gatcggcgta gctatgattg tcggcttctc tttccaggga accgagctga
    17101 tcggtattgc tgcaggcgag tccgaagatc cggcgaaaaa cattccacgc gcggtacgtc
    17161 aggtattctg gcgaatcctg ttgttctatg tgttagcgat cctgattatc agcctgatta
    17221 ttccgtatac cgatccgagc ctgctgcgta acgatgttaa agacatcagc gtcagtccgt
    17281 tcaccctggt gttccagcac gcgggtctgc tctctgcggc ggcggtgatg aacgcagtta
    17341 ttctgacggc ggtgctgtca gcaggtaact ccggtatgta tgcgtctact cgtatgctgt
    17401 acaccctggc gtgtgacggt aaagcgccgc gcattttcgc taaattgtcg cgtggtggcg
    17461 tgccgcgtaa tgcgctgtat gcgacgacgg tgattgccgg tttgtgcttc ctgacctcca
    17521 tgtttggcaa ccagacggta tacctgtggc tgctgaacac ctccgggatg acgggtttta
    17581 tcgcctggct ggggattgcc attagccact atcgcttccg tcgcggttac gtattgcagg
    17641 gacacgacat taacgatctg ccgtaccgtt caggtttctt cccactgggg ccgatcttcg
    17701 cattcattct gtgtctgatt atcactttgg gccagaacta cgaagcgttc ctgaaagata
    17761 ccattgactg gggcggcgta gcggcaacgt atattggtat cccgctgttc ctgattattt
    17821 ggttcggcta caagctgatt aaaggaactc acttcgtacg ctacagcgaa atgaagttcc
    17881 cgcagaacga taagaaataa gttttctccc ttccttgcta agccctctca accgagaggg
    17941 ctttttcaat tccatttccc tgacaaatca tgcggatata aaatttaaca tttggattga
    18001 taattgttat cgtttgcatt atcgttacga cgcaatcaaa aaaggctgac aaatcagagg
    18061 ctgttccggc tttctgggat gatcacctgc atacaaataa gtccaccgcg atgctgccgt
    18121 acgcaagggg acgtaaagaa gatgtgagcg atacccattt tattttcgta gttacctcat
    18181 ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt cgggctgtgt ttgtccgcta
    18241 tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg cgaaacgatg gttgtcactg
    18301 catcttccgt ggaacaaaat cttaaagatg cacccgccag tatcagcgtc attacccagg
    18361 aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt cctcaaagaa gtgcctggcg
    18421 tacaactgac gaacgaaggg gataaccgta agggcgttag tattcgtggt ctggacagca
    18481 gctacaccct gattcttgtc gacggtaaac gcgttaactc ccgcaatgcc gtcttccgcc
    18541 acaatgattt cgatctgaac tggatcccgg tcgattccat cgaacgtatt gaagtggtgc
    18601 gtggcccgat gtcgtcgcta tacggctccg atgcgctcgg cggtgtagtg aatatcatca
    18661 ccaaaaaaat cggtcagaaa tggtcgggta ccgttaccgt cgataccacc attcaggaac
    18721 atcgcgatcg cggtgatacc tataacggtc agttctttac cagtggacca ttaattgatg
    18781 gtgtgctggg aatgaaagct tacggcagcc tggcaaaacg tgaaaaggat gacccgcaaa
    18841 actcaacgac caccgatacc ggagaaacgc cgcgtattga aggattctcc agccgcgacg
    18901 gcaatgtcga atttgcctgg acaccgaatc aaaatcacga ttttactgcc ggatacggtt
    18961 tcgaccgtca ggatcgtgat tccgactcgc tggacaaaaa ccgcctggaa cgccagaact
    19021 actgcgtcag ccataatggg cgttgggatt acggcaccag cgaactgaaa tcctacggtg
    19081 agaaagtcga gaacaaaaac cctggcaaca gcagcccgat aacttccgaa agcaatacgg
    19141 tcgacggcaa atacacgttg ccgctgacgg cgattaatca gtttctcacg gttggcggtg
    19201 aatggcgtca cgacaaactt agcgatgcgg tgaacctgac cgggggaacc agctccaaaa
    19261 cgtctgccag ccagtacgcg ctgtttgtgg aagatgaatg gcggatcttc gagccgctgg
    19321 cgctgacgac cggcgtgcgt atggacgatc acgaaaccta cggtgaacac tggagtccgc
    19381 gtgcctacct ggtttataac gccaccgaca ccgtaacggt gaaagggggc tgggcgacgg
    19441 catttaaagc accttctctg ttgcaactta gccctgactg gacgagcaat tcctgccgtg
    19501 gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga aaccagcgaa agttgggagc
    19561 tggggcttta ctacatgggt gaagaaggct ggctggaagg gggtgaatcc agcgttatcg
    19621 ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg tacgtctgac gtcaacgctg
    19681 caccgggcta ccaaaacttt gttggttttg agacgggcgc taacggacgg cgcataccgg
    19741 tatttagcta ctacagcgtt aacaaagctc gtattcaggg cgtggaaacc gaactgaaaa
    19801 ttccgttcaa cgatgaatgg aaactgtcga tcaactacac ctacaacgat ggtcgtgatg
    19861 tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt ccatactgct aacggtacgc
    19921 tggactggaa accgctggcg ctggaagact ggtcattcta tgtttctggt cactataccg
    19981 ggcagaaacg cgccgacagc gcgacggcta aaacaccggg cggttatacc atctggaata
    20041 ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg cgcaggcgtg ctgaaccttg
    20101 gcgacaagga tctcagtcgt gacgactaca gctataacga agacggacgt cgttacttta
    20161 tggcagtgga ttatcgcttc tgatgagaag atgcccggcg aaccgggcgg actttcactt
    20221 cagtaaatac tgcgcatgga agcgcaggtg atcctctata aaagaggcga tgaagtagta
    20281 actgtgatca tatcccggct gaatacgcag cgtcatcggc cacgctttct ggcgcgcggc
    20341 ttctgccaat accgcaggtt gcaactggtc ggcaagaaac tgatcattat cgccctgatc
    20401 gataagcgtc gggatcgcat cctgcgcgtt actggcatac atcagcgcgc aactatccca
    20461 ttccagccat gcatttttgt cctcacctaa ataggtgcta aacgctttga ttccccacgg
    20521 gacgctgcac ggattcacaa tgggcgcaaa ggccgaaacg ctggtgtatt tacccggatt
    20581 tttcagcgcc ataatcagcg caccgtgacc acccattgag tgaccgctaa tggcgcagcg
    20641 gtcgctgaca ttaaattgcg actgaaccag cgccggtaat tcatcgcgca gataatcata
    20701 catccggtaa tacgtcgccc acggcggttg cgtggcatta agataaaagc ctgcgccctg
    20761 gcctaaatcg tagccatcgt cgttggcaac cttttcgccg cgcgggctgg tgtctggcat
    20821 caccagtaca atccccagtt ccgccgctac ccgctgggca cccgccttgg tggtgaagtt
    20881 ctcgtcattg caggttaatc cggaaagcca gtacagcact ggtggcgggg tgtgatcacg
    20941 agggggtggg agaaagatac tgaacgtcat cgggcagttt aaggtactgg agtcgtgtcg
    21001 ccagcgttgc tgccagcctt caaaacagcg gtgctcttcg agcatttcca tgcgtggctc
    21061 cttgttgtgt tgtttgcaat ttcttcatca taccgtttat tcaatgtgct gtgagtaact
    21121 ttcacttccg tatttgcata acgatgtttt aacatctgct gatgaaaggc agcggcaatt
    21181 acaataatta tcgctgtgaa tactggatta tgtgcgccgc ctcacgcaca ataatcaagc
    21241 tgtaaatcag cttaataact ttgcccccac gcagggcgga ggcgtcacac ctgcaggaga
    21301 aatcataaat gccatcactc agtaaagaag cggccctggt tcatgaagcg ttagttgcga
    21361 gaggactgga aacaccgctg cgcccgcccg tgcatgaaat ggataacgaa acgcgcaaaa
    21421 gccttattgc tggtcatatg accgaaatca tgcagctgct gaatctcgac ctagctgatg
    21481 acagtttgat ggaaacgccg catcgcatcg ctaaaatgta tgtcgatgaa attttctccg
    21541 gtctggatta cgccaatttc ccgaaaatca ccctcattga aaacaaaatg aaggtcgatg
    21601 aaatggtcac cgtgcgcgat atcactctga ccagtacctg tgaacaccat tttgttacca
    21661 tcgatggcaa agcgacggtg gcttatatcc cgaaagattc ggtgatcggt ctgtcaaaaa
    21721 ttaaccgtat cgtgcagttc tttgcccagc gtccgcaggt gcaggaacgt ctgacgcagc
    21781 aaattcttat tgcgctacaa acgctgctgg gcaccaataa cgtggctgtc tcgatcgacg
    21841 cggtgcatta ctgcgtgaag gcgcgtggca tccgcgatgc aaccagtgcc acgacaacga
    21901 cctctcttgg tggattgttc aaatccagtc agaatacgcg ccacgagttt ctgcgcgctg
    21961 tgcgccatca caactgatta aaaggcagga accatggagc gcaacgtcac gctcgatttt
    22021 gttcgcggcg tcgccattct ggggatcctg ctattaaaca tcagcgcctt tgggctacca
    22081 aaggcggctt atctcaatcc cgcctggtac gatgctatta cgccgcagga tgcatggacc
    22141 tgggcatttc tcgatctcat cggccaggtg aaattcctca cgctttttgc gctgctgttt
    22201 ggtgctggcc tgcaaatgtt gctgccccgt ggcagacgct ggatccagtc acggttaacg
    22261 ctgttagtct tgttgggctt tattcacggt ttattgttct gggacggcga tattctgctg
    22321 gcttacgggc tggtgggctt aatctgctgg tggctggtgc gcgatgcgcc atcggtaaaa
    22381 agcctgttta ataccggcgt catgctttat ctggtggggc ttggcgtttt gctgttattg
    22441 gggctgattt ccgacagcca gaccagccgc gcctggacgc cggatgcatc ggctattttg
    22501 tatgaaaaat actggaagct tcacggcggc gttgaagcga tcagtaatcg tgccgatggt
    22561 gttggcaaca gtttactggc actgggcgca cagtatggct ggcaactggc agggatgatg
    22621 ctcattggtg cggcgctgat gcgcagcggc tggctgaaag ggcagttcag cttacgtcac
    22681 tatcgtcgta ctggttttgt gctggtggcg attggggtga ccattaacct tcctgccatc
    22741 gccctgcaat ggcagctgga ctgggcgtat cgctggtgtg ccttcttact tcaaatgccg
    22801 cgggaactga gtgcgccgtt tcaggcgatt ggctatgcgt cgctgtttta tggcttctgg
    22861 ccgcaattga gccgctttaa gctggtgctt gcgatcgcct gcgtcggacg gatggcgctg
    22921 accaactatc tattgcaaac gctgatttgt accacgcttt tttaccacct cggtttgttt
    22981 atgcattttg accgcctgga gctgctggcg tttgttattc cggtatggct ggcgaatatc
    23041 ctcttctctg ttatctggct gcgttacttc cgccaggggc cggtggaatg gctctggcgt
    23101 cagttaactt tgcgtgctgc cggactggca atatctaaaa catcaagata acgatctgga
    23161 tcacattcgt taacaaaacg gctgtaaccg tttccattgc tgtgactcga ttcacgaagt
    23221 cctgtattca gtgctgacaa aatagccgcc agcaagcagt catttactgc aatctcataa
    23281 caggtagtga atatgatcac cattcgtgat gtagcgcgtc aggctggcgt ctctgtggca
    23341 acggtttccc gggtgctcaa taacagcacg ctggtcagtg ccgacacgcg tgaagcagta
    23401 atgaaagccg tgagtgagct ggattatcgg ccaaacacca atgcccaggc gctggcaact
    23461 caggttagcg acaccattgg cgtggtggtg atggacgttt ctgatgcgtt tttcggcgcg
    23521 ctggtaaaag cggtggatct ggtcgcccag cagcatcaga aatacgtgct aatcggcaat
    23581 agctatcatg aagcggaaaa agagcgtcac gccattgagg tgttaattcg ccagcgttgt
    23641 aatgcgttga ttgttcactc aaaagcattg agtgacgatg aactggcaca atttatggat
    23701 aacattcccg gtatggtgtt aatcaaccgc gttgtgccgg ggtacgccca tcgttgcgtt
    23761 tgcctagata atctcagcgg tgcccgaatg gcgacgcgca tgttgctgaa taacggtcat
    23821 caacgtattg gttatctttc ttccagccac ggcattgaag atgacgccat gcgtaaagca
    23881 ggctggatga gtgcgttgaa agagcaggat attattccgc cggaaagctg gattggcact
    23941 ggtacgccgg acatgccggg cggtgaggcg gcgatggttg aactgctggg gcgcaatcta
    24001 caacttaccg ctgtatttgc ttataacgac aatatggccg ctggcgcact gacagcatta
    24061 aaagataatg gcattgcgat tccgttacat ctctcaatca tcggtttcga tgatattccc
    24121 atcgcccgtt acaccgaccc gcaattaacg accgtgcgtt atcccattgc ttcaatggct
    24181 aaattagcca ccgaactggc cttgcagggg gcagcaggca atattgatcc tcgtgccgcc
    24241 actgttttat gccgacgtta gtgcgtcgcc attctgtcgc aacgcgccag aatgcggcgg
    24301 cgatcactaa ctcaacaaat caggcgatgt aaccgctttc aatctgtgag tgatttcaca
    24361 gtatcttaac aatgtgatag ctatgattgc accgttttaa tgttgtaacc cgtatgtaac
    24421 agtgaataat cactttcacc gaggtaatag cgtcataaca acaattaaag ccgttttctg
    24481 gagcgttacc gggcatggaa gaacgaattt taaaaagtga gcttcggcgt tcagtaacac
    24541 ttcattaact ctactgcccc gccgagcatt tatctcaagc actaccctgc ataagaaaaa
    24601 ccggagatac catgaataag aaggtgttaa ccctgtctgc tgtgatggcc agcatgttat
    24661 tcggtgccgc tgcacacgct gctgatactc gcattggtgt aacaatctat aagtacgacg
    24721 ataactttat gtctgtagtg cgcaaggcta ttgagcaaga tgcgaaagcc gcgccagatg
    24781 ttcagctgct gatgaatgat tctcagaatg accagtccaa gcagaacgat cagatcgacg
    24841 tattgctggc gaaaggggtg aaggcactgg caatcaacct ggttgacccg gcagctgcgg
    24901 gtacggtgat tgagaaagcg cgtgggcaaa acgtgccggt ggttttcttc aacaaagaac
    24961 cgtctcgtaa ggcgctggat agctacgaca aagcctacta cgttggcact gactccaaag
    25021 agtccggcat tattcagggc gatttgattg ctaaacactg ggcggcgaat cagggttggg
    25081 atctgaacaa agacggtcag attcagttcg tactgctgaa aggtgaaccg ggccatccgg
    25141 atgcagaagc acgtaccact tacgtgatta aagaattgaa cgataaaggc atcaaaactg
    25201 aacagttaca gttagatacc gcaatgtggg acaccgctca ggcgaaagat aagatggacg
    25261 cctggctgtc tggcccgaac gccaacaaaa tcgaagtggt tatcgccaac aacgatgcga
    25321 tggcaatggg cgcggtagaa gcactgaaag cacacaacaa gtccagcatt ccggtgtttg
    25381 gcgtcgatgc gctgccagaa gcgctggcgc tggtgaaatc cggtgcactg gcgggcacag
    25441 tactgaacga tgctaacaac caggcgaaag cgacctttga tctggcgaaa aacctggccg
    25501 atggcaaagg tgcggctgat ggcaccaact ggaaaatcga caacaaagtg gtccgcgtac
    25561 cttatgttgg cgtagataaa gacaacttgg ctgaattcag caagaaataa gactgattgg
    25621 gcgcagtcgt ttactgcgcc cttattgtat ggccaacaag gtaaaattat atggtcagct
    25681 caacgactcc gtcttccggg gaatacttgt tggaaatgag cggtatcaac aagtcctttc
    25741 ctggtgttaa ggcacttgat aacgttaatt taaaagtccg gccacattct atccatgcat
    25801 taatggggga aaacggtgca ggaaaatcga cattattaaa atgcctgttt ggtatttatc
    25861 aaaaagactc cggcaccatt ttattccagg gtaaagagat cgatttccat tccgccaaag
    25921 aggcactgga aaatggtatt tcgatggtac accaggagtt aaacctggta ttacaacgtt
    25981 cggtgatgga taacatgtgg ctggggcgat atcccaccaa aggcatgttt gtcgatcagg
    26041 acaaaatgta ccgcgaaacc aaagcgattt ttgatgaact ggatattgat atcgatccgc
    26101 gtgcgcgcgt cggcacatta tccgtttcgc aaatgcagat gatcgaaatc gccaaagcgt
    26161 tttcctataa cgcgaaaatt gtgattatgg atgaaccgac ttcttcgtta accgaaaaag
    26221 aggtcaatca tctgttcact attattcgta aattaaaaga gcgcggctgc ggtattgttt
    26281 atatctcgca taaaatggaa gaaatcttcc agttatgtga tgaagttacc gtattgcgcg
    26341 acggtcagtg gatcgccacc gaaccgctgg caggactgac gatggacaag atcatcggca
    26401 tgatggttgg gcgttctctt aatcagcgtt ttcctgacaa agaaaacaag ccgggcgaag
    26461 tcatcctcga ggtgcgtaac ctaacgtcac tgcgccagcc gtcgattcgc gatgtctcgt
    26521 ttgatctgca taaaggggag atcctcggta ttgccgggct ggtgggggcg aaacgtaccg
    26581 atattgttga gacgttattt ggtattcgcg agaaatcggc tggcaccatt acgttgcacg
    26641 gcaaacagat caataaccat aatgccaacg aagccataaa ccacggattt gcactggtaa
    26701 ctgaggagcg ccgctcaacg ggaatttatg cctatctgga tattggtttt aactcgttaa
    26761 tttccaatat tcgcaactac aaaaataaag ttggtttact ggataactcg cggatgaaaa
    26821 gcgataccca gtgggtgatt gattcgatgc gggtaaaaac gccgggtcat cggacgcaaa
    26881 ttggttcgct ctccggtggt aatcagcaaa aggtgattat tggtcgctgg ctactaacgc
    26941 aaccagaaat attaatgctc gatgaaccga cgcgcggtat tgatgtcggg gcgaagtttg
    27001 aaatttatca gttaattgcc gaactggcga agaaaggcaa ggggattatt attatctcct
    27061 ctgaaatgcc tgagttgtta gggataacag accgtattct ggtcatgagc aatggtctcg
    27121 tttccggaat tgtcgataca aaaacaacaa cgcaaaacga aattctgcgt cttgcgtctt
    27181 tgcaccttta agattagggg cttcccatga gtgcgttaaa taagaaaagt tttcttactt
    27241 acctgaaaga gggcggtatt tacgtcgttc ttttagtttt gctggcgatt attattttcc
    27301 aggacccaac atttttaagt ctgttgaact taagtaatat tctcacccag tcatcggtgc
    27361 gtattattat cgcgctcggt gtggcagggt taattgtcac ccaggggacc gatctttctg
    27421 ctggtcgtca ggtagggctg gcggcagtgg tggctgcgac attattgcag tccatggata
    27481 acgccaacaa agtgttcccg gaaatggcga cgatgccgat tgcgctggtt attctgattg
    27541 tctgtgccat tggtgcggtg atcggtttga tcaacggtct gattatcgct tatctcaacg
    27601 tgacgccgtt cattaccacg ctcggcacga tgatcatcgt ctatggcatc aactcgctct
    27661 attacgactt tgtcggggcg tcgccaattt ctggttttga cagtggcttc tctacctttg
    27721 ctcagggctt tgtcgcgctg gggagtttcc gtctctctta catcaccttc tacgcgttga
    27781 ttgcggtggc gttcgtctgg gtgttgtgga acaaaacccg cttcggtaag aacatttttg
    27841 ccattggcgg taacccggaa gcggcaaaag tatctggtgt caacgtcggc ctgaacctgc
    27901 tgatgatcta cgcgttgtct ggcgtgttct atgcctttgg cgggatgtta gaagccggac
    27961 gtatcggctc tgccaccaac aacctcggct ttatgtatga gctggatgct atcgcggcgt
    28021 gcgtggtagg cggtgtatcg ttcagcggcg gtgtggggac ggtgattggc gtggtgaccg
    28081 gggtaattat ttttaccgtc atcaactatg gcctgacgta tatcggcgta aacccatact
    28141 ggcagtacat catcaaaggg gcgattatta tcttcgccgt agcgctggat tcactgaaat
    28201 acgcgcgtaa gaagtgatgt gattgcggat attgccggat gcgtctctgg ttttacaacg
    28261 ttaccgggtg ttctttctcg cctttcttaa acttcacttc cccgagctca atacaaccca
    28321 ccgggcagac gtgaccacaa agcagacaac ccacacattt ctcggtatta caatgcggtg
    28381 tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac
    28441 agcgtccaca gccaacacat ttgtcaagat tgatacgggg atagacaata taactgcggt
    28501 ctaaatcttc cgccgggaca atattgttat tcgccagacc taccatctcc tgcaacgaat
    28561 caaatccctg atcggcgaga taatgcgata acccgctcgc catatcttct acgatgcgat
    28621 agccgtactg cataatgccg gtggtgacct gtaacgttgc tgcgccgagc aataaaaact
    28681 ccgccgcatc ttcccaggtt tcaatgccgc cgataccgct gattgggaaa tcgcgcagtt
    28741 ctggatgggt gcgcatttgc tgaatgaagc gcagggcgat cggttttacc gctttaccgg
    28801 aatatccgga aatactcgat ttaccgttaa cgatcggcat accgattttc tgattaagat
    28861 cgatattggt gatggattta acggtgttaa ttgccgcaat gccatctgcg ccgccgcgct
    28921 tcgccgccag cgccacttcg cacatatcac cgatattcgg cgtcatcttc gccagcattg
    28981 gcagcgtgga accccgtttc actgcccgac aatatttttt taccagctcc gggctttgcc
    29041 cgacatcgct acccatcgca tgagaagtca tttgcggaca ggagaagtta cactcgatca
    29101 tatccgcgcc agcttcttgc accaggcgcg ccagctcctc ccattgctgc tcattttccc
    29161 ccatgatcga agcgatcaat actttgtccg ggtaatcttc cttcagccga cgcagggcgg
    29221 tcagattctc ttccaacgga tgttcagcaa tctgctccat atttttgaag ccgataaaac
    29281 cggtatcttc tttcaccaga tgatcaaaac gcggcgagac ttcgttggcg ataaaaaagc
    29341 cgatcgtttt aaacaccaca ccgccccaac ctgtgtcgta ggctttggca cacatctcat
    29401 agcagttgcc taccggcgaa gaagagaggc agaacgggtt gggaaagttc acgccgcaaa
    29461 aagtaatcga aagatctttc gttaacatga gcaagctccc tctaatatag tgatgaatcg
    29521 cctcggcggc ttctttcccg gttttcacgg catagaccac ggttttgtca ccctcaacaa
    29581 tatcgccagc agcaaagact tgcggatcgc gggtctgata attttgtgtt ttgatggtgt
    29641 tacgctgctg ctctaactcc gcaaaggcat ccagtctggc atgctgaccg acggcgagaa
    29701 taattttatc tgccgccatc gtcagttcgc ctgataaccg tacatgctta aacgtgactt
    29761 tattgccttc cacggctact ggcgtgaatc catcgatgat cgaaacgccc agctcccggg
    29821 cgctggcaaa ctctttttcg cttgccggaa actcatctaa ctcttcacgt gctacgcaag
    29881 ttaccgcctg acagccgaga actttcagcg tgctggctac gtccatcgcg acatcaccgc
    29941 cgccgataat taatgcgctt tgtggaatgc tgatatcgcc ttgcgcctgt cgtacacgtt
    30001 gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa cagcggtagc ccggaaccgc
    30061 ttgataaccc cacggtgacc agtaccgcgc ggttttccgc tttcagttgc tccagggtga
    30121 gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc cattttttca atacgggcga
    30181 tctctgcatc cagcactgat tgcggtaacc ggaattgcgg aataccgtta cgcaaccagc
    30241 caccggggtg cgcttctttc tcataaatcg tgacgtcata accctggttt gtcagtgtca
    30301 cactggcctg caatccggca ggacctgcgc caataatcgc gactttgccg agcgttttag
    30361 taccgggctg ataaatttcc atcccggttt gttgttcaaa atcagtaaca aaacgctgta
    30421 agcggccgat atcaatggga gcatcaacac cggcacgggt acaaccgctt tggcataatt
    30481 tctccgtcgg gcacactctg gcacaaacgg caccgagggc attattttcg cgaattgtct
    30541 cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat aaatttcccc ggatcggtct
    30601 gtgccgggca agcctgacta cagggagcgt cgtgacataa taaacagcga gaggcttctt
    30661 taatcgccag taacggcgta aaagccggag tgagttcatc cagataattt tgttgcggca
    30721 tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc taaataaata ttgcgtgctg
    30781 atattaaggc tatgcgcgtg gggaaattta aactgtgaaa ggattcacaa atagctgcgg
    30841 ttcagaatgt tatatccgca gcttattgag tgaagtaata attattctca ataattaaca
    30901 gaaaagagaa aatattaacg atagaaaata agataaacaa tcatgccgat agataaaatt
    30961 ccgaagccaa tatatgccac catataagcg aagaagagtc cgggattttt tgctcgccag
    31021 acatacaccg gttccggtgc atttttaacc cgtttatcaa tcgcgcctgc gcgccagcct
    31081 ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa aaacggcaac gacaaaaaac
    31141 tgctgtacat ccataactac tttccttgtt tcttttgtaa ttcaagtaac tgttcgggtg
    31201 taacggcggg atacccctgc gcatcttccg gcacctggtg catagccgga atagggacca
    31261 gcggccctaa aaaacgcggt tcacgtttaa aaatataaag gtcagccagc gcaccgaaac
    31321 gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt cggtgacggt acggcataac
    31381 actgagcctg aatccccata tgcagcgcaa taaataatgc tcgctcacag tggaaacgtt
    31441 gggtgataat aatgaaatca ttagtatcga aaactttgcg tgtacgcacg atggaatcca
    31501 gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg gtcgacacca gcagcgatta
    31561 aatctttgcg catggtcatc ggctcgttat aactttgcaa tgcgttatcg ccgctcagta
    31621 atagataatt taccttaccg ctgttatagg cattaatcgc tccttgaatg cggtagcgat
    31681 aatactgatt aataacgccg gtacgataat attttgctgt gccgagcacc acgccgacct
    31741 ggcggtaggg gagatcctgc aattcgtcgt agatataagg cgcggttttc cagctcatcc
    31801 agcgatcgag gccgagcaca gtcaacagca gcaagccgat caggactaac aggctgagga
    31861 acacgcgctt taacatgcag gagactcgag ctaaggaaaa tttccatcag gctactttac
    31921 ccgctggttt tgcgcaagaa acggcaaact gtttgccggg aatttcgaca ccaggagagg
    31981 gctggcaagc cggacaaggc gttcacgccg catccggcac caggcttaag cgagaagcac
    32041 gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg gaggtggcat cccactgaat
    32101 caacggcgca tcggcttttt ctgccagaac cgcgcgctgg atatccgggt aatcataacc
    32161 cttgagattc aacagaatta acgccccctg caacggcggc agagtcgggt tgaatgcggc
    32221 gttttcagcg tagctgccgc tgaaaatacg accgtcttta cattccagcg cgacaccgct
    32281 tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct gcaatcgccg cctgagaaag
    32341 cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc atcagcagcg ttttaatctc
    32401 cagatctttc ggcccaaagg catccggcag atagtcacgc agcgcgtgtg cctcgcggcc
    32461 cggcaaatga atacgcagat ccagaccgct gttcagttca ttcataaact gacggcagtg
    32521 accacaaggc gtatagttaa cggtgatggc tgcaagcgct ttttcaccac tcaaccaggc
    32581 gtggctgatc gcgctttgtt cggcatgaac ggtttgctgc attgttgcac caataaattc
    32641 catattggca ccaaaatacc aggttccgct cacaccgcgc gcaattgcgc caacattaaa
    32701 attcgacaat ggcgtacgcg cacaggccgc cgccagcgga agtagtgcga atgccagcgc
    32761 gtcttcgtcc agccccgttg cgctcttcag cgatgagact tgctccccgg tcaacaaagc
    32821 ggggaagtac ttgtctgcca gaataggttc cagtgcagat tgcaagttat ccgcaagttg
    32881 ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct cataatcatg taatgggata
    32941 acagtttacg aacccgttat ggctttatat gtgatctgaa tctcattaat tatgcaacac
    33001 gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt tagcccatta ctgccagaat
    33061 aatcgggaaa aggaacggtg cgatcagcga agtaattatc ccgcataaca ccagcgctag
    33121 cgaactaaat gcaccttcct gataatccag ctcggcgcag cgcgccgtac cgagggcgtg
    33181 cgaggcagtc cccattgcca gaccgcgcgc agctttggta cgaatacgca tcgcattaag
    33241 caatgtatgg ccaaataccg cgccgaggat gccgacgaaa atcacgcaaa ctgcgctgat
    33301 tgccggaata ccgccaatac tgccgccaac cgacattgca ataggcgtgg tgactgattt
    33361 cggcaggatt gacgcggtga tttccggtga agcgcccatc aacaatgcca cggaagtccc
    33421 cgtcaccatc gcaaccacgc tgccgataaa acagatggtg atgatcgatt tccagcgcgc
    33481 gcggatctgg tgtagctgct catataaagg ataggccagc gcgacgaccg ccggttgcag
    33541 cagatcgttt agcacttcgc tacctttaaa gtaactgtcg taagagatgc cggtcagcat
    33601 taaaaaagga atgatcacca ccattgctac cagcaacggg ttaagcaggg gaaacttata
    33661 ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac gtcagcggta atgaccacca
    33721 gatattcgcc atcattcttc tgatcctttc tgacctacga ctttacgttc accgtgtacc
    33781 agttgtgaac tccagctcac caccagaaaa accaccagcg tactgacagc acaggaaacc
    33841 actaccgggc caaactgcgc gcggagcaaa tcaaaatatt gcatgacgcc tacgccaatc
    33901 ggcacaaaca atagcgccat atagcgaatc agtacgtagc accccggatt gacccatttc
    33961 gccggaagaa tttgcaaggc cagcaggaca aacaggatca gcatcccgat gatgctgccc
    34021 ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg catacaggca ggcataaatc
    34081 aggacgaaag cgcgtaaata ttgccagata atgttcagtg tcttactcat aatcaacacc
    34141 ccagatgaga tggattcatc atacaattaa ttcgtaaaac gtgctaccga tcacattaag
    34201 aaatcagcgc ttattttttc gctacccggt attacgcaat gagcagcgaa ctcaggcata
    34261 catcaagcat agagagggat tgcagtttgc agcaatccct ctttgcagac ctttaatccg
    34321 ctgaccatag gcgctaacat agcgcctcat ttttttgcgg gtgatgatat acgtgtgtta
    34381 ctggcaccga tggagggagt gcttgactct ctggtgcgtg aattgctgac cgaagttaac
    34441 gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg atcaactgct gccggtaaaa
    34501 gtctttcatc gcatttgccc tgagctacaa aacgccagcc ggacaccatc tggtacgctg
    34561 gtgcgcgtgc agttgttagg tcagttccca caatggctgg cagagaacgc cgcccgtgcg
    34621 gtggagttag gttcctgggg cgtggatctc aattgcggct gcccgtcgaa aacggttaac
    34681 ggtagcggcg gcggggcgac gttactcaaa gatcctgaac tcatctacca gggggcaaaa
    34741 gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg tgaaagtgcg tctgggctgg
    34801 gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc aacaggctgg cgctacggag
    34861 ctggtggtgc atgggcggac gaaagagcag ggttaccgcg cggagcatat tgactggcag
    34921 gcgattggcg agattcgcca gcggctgaat attccggtga ttgccaacgg tgaaatctgg
    34981 gactggcaga gcgcgcaaca atgcatggcg atcagcggct gcgacgcagt gatgattggt
    35041 cgcggggcgc tcaatattcc caacctgagc cgggtggtaa aatataacga accgcgaatg
    35101 ccgtggccgg aggtggttgc tttgctgcaa aaatataccc gtctggaaaa gcagggcgat
    35161 accgggttat atcacgttgc gcggattaaa cagtggttga gttatttgcg taaagaatac
    35221 gatgaagcaa cggaattatt tcagcatgtt cg
//
LOCUS       DADWTH010000035        35035 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.035, whole genome shotgun sequence.
ACCESSION   DADWTH010000035 DADWTH010000000
VERSION     DADWTH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.035"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="JNY02_001789"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="JNY02_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBB9433346.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="JNY02_001790"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="JNY02_001790"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBB9433347.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="JNY02_001791"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="JNY02_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBB9433348.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNY02_001792"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNY02_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBB9433349.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="JNY02_001793"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="JNY02_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNY02_001794"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNY02_001794"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBB9433350.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNY02_001795"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNY02_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBB9433351.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="JNY02_001796"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="JNY02_001796"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBB9433352.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="JNY02_001797"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="JNY02_001797"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBB9433353.1"
                     /translation="MRFDTVIMGSGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNKENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQITQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="JNY02_001798"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="JNY02_001798"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBB9433354.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13532
                     /locus_tag="JNY02_001799"
     CDS             12630..13532
                     /locus_tag="JNY02_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBB9433355.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13573..14043)
                     /locus_tag="JNY02_001800"
     CDS             complement(13573..14043)
                     /locus_tag="JNY02_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBB9433356.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14098..15300)
                     /locus_tag="JNY02_001801"
     CDS             complement(14098..15300)
                     /locus_tag="JNY02_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBB9433357.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15387..15929)
                     /locus_tag="JNY02_001802"
     CDS             complement(15387..15929)
                     /locus_tag="JNY02_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433358.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16208..16633
                     /gene="nudI"
                     /locus_tag="JNY02_001803"
     CDS             16208..16633
                     /gene="nudI"
                     /locus_tag="JNY02_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBB9433359.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16672..17274)
                     /locus_tag="JNY02_001804"
     CDS             complement(16672..17274)
                     /locus_tag="JNY02_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB9433360.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17582..18721
                     /gene="arnB"
                     /locus_tag="JNY02_001805"
     CDS             17582..18721
                     /gene="arnB"
                     /locus_tag="JNY02_001805"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBB9433361.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18725..19693
                     /gene="arnC"
                     /locus_tag="JNY02_001806"
     CDS             18725..19693
                     /gene="arnC"
                     /locus_tag="JNY02_001806"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBB9433362.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19693..21675
                     /gene="arnA"
                     /locus_tag="JNY02_001807"
     CDS             19693..21675
                     /gene="arnA"
                     /locus_tag="JNY02_001807"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBB9433363.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYDS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21672..22562
                     /locus_tag="JNY02_001808"
     CDS             21672..22562
                     /locus_tag="JNY02_001808"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBB9433364.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22562..24207
                     /gene="arnT"
                     /locus_tag="JNY02_001809"
                     /pseudo
     CDS             22562..24207
                     /gene="arnT"
                     /locus_tag="JNY02_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
     gene            24204..24539
                     /gene="arnE"
                     /locus_tag="JNY02_001810"
     CDS             24204..24539
                     /gene="arnE"
                     /locus_tag="JNY02_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBB9433365.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24539..24925
                     /gene="arnF"
                     /locus_tag="JNY02_001811"
     CDS             24539..24925
                     /gene="arnF"
                     /locus_tag="JNY02_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBB9433366.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24919..25191)
                     /gene="pmrD"
                     /locus_tag="JNY02_001812"
     CDS             complement(24919..25191)
                     /gene="pmrD"
                     /locus_tag="JNY02_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBB9433367.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25296..26651)
                     /gene="menE"
                     /locus_tag="JNY02_001813"
     CDS             complement(25296..26651)
                     /gene="menE"
                     /locus_tag="JNY02_001813"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBB9433368.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26648..27610)
                     /gene="menC"
                     /locus_tag="JNY02_001814"
     CDS             complement(26648..27610)
                     /gene="menC"
                     /locus_tag="JNY02_001814"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBB9433369.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFTKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27610..28467)
                     /gene="menB"
                     /locus_tag="JNY02_001815"
     CDS             complement(27610..28467)
                     /gene="menB"
                     /locus_tag="JNY02_001815"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBB9433370.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDICYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28482..29240)
                     /gene="menH"
                     /locus_tag="JNY02_001816"
     CDS             complement(28482..29240)
                     /gene="menH"
                     /locus_tag="JNY02_001816"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBB9433371.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29237..30907)
                     /gene="menD"
                     /locus_tag="JNY02_001817"
     CDS             complement(29237..30907)
                     /gene="menD"
                     /locus_tag="JNY02_001817"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBB9433372.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30996..32291)
                     /gene="menF"
                     /locus_tag="JNY02_001818"
     CDS             complement(30996..32291)
                     /gene="menF"
                     /locus_tag="JNY02_001818"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBB9433373.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAKGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGERLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32370..32675)
                     /gene="elaB"
                     /locus_tag="JNY02_001819"
     CDS             complement(32370..32675)
                     /gene="elaB"
                     /locus_tag="JNY02_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBB9433374.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32730..33191)
                     /locus_tag="JNY02_001820"
     CDS             complement(32730..33191)
                     /locus_tag="JNY02_001820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB9433375.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDCIPHIGMAREVIQA"
     gene            33256..34173
                     /gene="rbn"
                     /locus_tag="JNY02_001821"
     CDS             33256..34173
                     /gene="rbn"
                     /locus_tag="JNY02_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBB9433376.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34361..35035
                     /locus_tag="JNY02_001822"
                     /pseudo
     CDS             34361..35035
                     /locus_tag="JNY02_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8599 a         9382 c         8647 g         8407 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa gggatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgaggaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgtt ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgagcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgctgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcagc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa taaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgcagatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attacccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gagataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ctttgcgcat gctggaacag ggcattgatc gtgaccaagt gctcgcggcc acccagctaa
    13501 gcgaagccga tctggcagcg aataaccact aattaacaca ggcccacagc cgatccccat
    13561 gggcctttga tatcaataac taccttttat gcgtggccca tttttgcctg atttaaacat
    13621 cgccagtcgt tgatccaggg catcgctgta gagcatcgtg tcaacgccaa cagcgacaaa
    13681 gttcgctccc cacgccaggc attgctgcgc catatcagga gccacagcca gaaaaccagc
    13741 cgctttaccc gcagcacgga tccgccgaat gctggtttca ataattcgct gcacttccgg
    13801 gtgcccggcg ttatccgggt agcccaacga cgcagaaaga tccgctggtc caataaacac
    13861 gccatcaatc ccttcgacgt cgaggatctc gtccaggtta tccagtgccg ttttactttc
    13921 cacctgcact aacagacaaa gcgaatcgtt gacttgcgcc atgtaattct caatgcgtcc
    13981 ccagcgcgca gcccgtgcca cactggcccc aacaccacgc tcaccgtagg gagggttgga
    14041 caaattttgc cggaggggga atgtgccggt gcagtaaacc ggcacagagt ggcgaaatca
    14101 gacggataaa gtcatggact caacaacctc aatccagcca tgctcactag cgatgtcctg
    14161 gccgtttaac caacggcgta gcatattcag tgccatcatt gcgcacactt cctgacggat
    14221 agccaggctg tagcgtgtag tgctgaaacg cacacgcaga gcgaaagtgc cgtctggcgt
    14281 ggctagcgca aagttgagat gctcgttctc gaaacccgaa acagccagtg ccagcccggc
    14341 aaaatggttg gcccgccgtt ctgtaatcca gtgcgcagtt tgcgccaggg tttcttcctg
    14401 tgaaggaatc acttcacacg ccagcaatgg agcacctgcg cgagaaagtt gcaaagccaa
    14461 taaaccaccg gtgaattgct cgctcaacgt caggctgaac tggcggtttt gcaattcgcg
    14521 actgatctgc gcgggcagtc cttcggtgcc ttcgaaaatc acgctctgtc cggcaacacg
    14581 tttaacgtcc agccacagtt tttccatcgc ctgttgctcg cttgccggtc cggtgagttt
    14641 cagttcgatg atcggcattg aggagcgata gcccattgtt acgcccggcg gcagttgtag
    14701 agtgtccagg ctttgtgcca gatcgctttc cgaacgacca aaggtagtta aacgcagaca
    14761 aaccggcggc tgtggtaaag aaaaacgctc gcgcaggcgc ggcaggattt cgtgctcgac
    14821 catcacctta aattctgacg gtacgccggg agtaaagaac atcaggcaac gattaagctg
    14881 cacggcaaaa ccacaggcgg tgcctaccgg gttattgata aattcagcac tggcaggcag
    14941 ctccgcttgt ttacggttgc tcggtgccat tactcgtcca cgttcgtgga aatagcgttc
    15001 catctctttg agccaggctt catgcagcac aaggccttca ccttttgctg tcgcagcggc
    15061 gagtgcgctt aaatcatcgc tggtcggtcc cagcccgccg ttaacgatca gcacatcggc
    15121 gtgctgacta cgttcgcgaa gaatggtgac taagtcatca aggttatccc ccaccgtatt
    15181 gcggcgagat aatggcagcc cctgatgaaa gaaaaaatcg gccagccagg cagcgttagt
    15241 gtcaacgatt tgcccgtata acacttcatc cccggtggat agcatttcca cttttaacat
    15301 ggttatctcc tgcttttgtt atcgaatcac tataacgcaa agggagataa ggcgtagtaa
    15361 gaaactgaat gccgcgccgg aagggatcag aaactggcgt taaccccaac atacaggccg
    15421 tcagccacag cgttgtcgcg gttaccgtct ttacccgaca gattcaggta gcgataaccc
    15481 gcctcaatac tgactggacg cataatggtg taacgcgcgc cagcattcgc ttcctcataa
    15541 ctttgaatac cgctggagag cgaatccgga gagtagtaat actcgccaaa caaacggaag
    15601 ctgttgccaa ttttccactg caaaccacct cctaccgctg cggcataacc ttcatcgccg
    15661 taattcgggt tggtgtacac gccttttccg ccaacggtcg ccattaacgg cccgagaggc
    15721 agattcaacc ccagccccac gcccgcgacg tcaccgtcgt cgtcgttatg tgtccagtta
    15781 ccgcttaaag ccaggcccgt cgattcagtc ccaaaaccga caccaatatt ggtgtattct
    15841 ttacccgcct ggccgctgat gctcattgca ttgaccgatg cagaaaccaa cagcattccg
    15901 gccagacctg ctagcgcaat ttttttcatt atcccctttc ccacaagcaa ttaatcaaaa
    15961 ttcccaaagc tgcgggattg tactgcctgg cacagaggat tcaatgagcg aaaaaaggga
    16021 tctagtcact tattgtaact aaatacttct taaataatta aacgccgaaa aaataaacac
    16081 actttaccga tacacacaaa aataataata agtgcagtta tcattgcaaa agagattact
    16141 gtcactttca catatttctg agttaccgta ttctcatggt ttctttttat ttataaggaa
    16201 tacccctgtg cgacaacgga ctattgtatg ccctttgatt caaaatgatg gtgcttattt
    16261 gctgtgcaaa atggccgacg atcgcggcgt tttccccggt caatgggcgc tttcgggtgg
    16321 cggcgtggag tctggcgaac gaattgaaga ggcactacgc cgcgaaattc gcgaagaact
    16381 gggagaacag ctgcttttga cagaaatcac gccgtggacc ttcagcgatg atattcgcac
    16441 caagacatat gcagatggtc gcaaggaaga gatttatatg atttacctga tttttgactg
    16501 cgtttctgcc aaccgagaag tgaaaataaa cgaagagttt caggactacg cgtgggtaaa
    16561 acctgaagat ctggtgcatt atgatttaaa tgttgccacc cgaaaaacgt tacgtttgaa
    16621 aggtcttctt taacaatgat gacagccatc actggctgtc agattgaaaa tttagtggtt
    16681 aacgaattcc ccatccagat aaactttgcc tttttccaca tgcatgacta aaccatccag
    16741 ataatcaggt ttaaatatcg catcacgctt atttttagca atatatgtca ggcaatgatt
    16801 atgggtgaaa ataacgatat ttttatcagg cgctttgctt tgtaagtcct taattgcact
    16861 ataaatctca ttaccgcact gaagaagtcg tttatctacc gtcagctttt tacccgcgga
    16921 aaaccaggtc gccgactgaa tggtccggac ggtattactg gaataaagat cgaaatcagg
    16981 gatgtcagcg ctaaaagcgt tgcccagttc acgggcatcc tgggtacctt taaccgtaat
    17041 acctgtttta tctgacaagc attgattggt tgaacgatcg caacgttcag catggcgaaa
    17101 caaaacgaca accgggtgct gctgtgccag tctggccaat gttttattgt cgatacgtgg
    17161 caaaccattg ctactccagg cggcatgagt acccagtccg gcaattgcag cgagcgccag
    17221 taaaatgatg aaatattttt ttgacttcaa tgatgagcgg caaaaagcta acacgtaaac
    17281 tccacctgta gacaagcgca accagacaat taccgtgaaa ttgagcgaca tttctggcga
    17341 taattagcag ttggtgtaat attaaaaatc ctacgatgtc ggcaaaatgc ctcaaatttt
    17401 tgccaaatgc aaagcctaaa taagaaaaaa tataaaaatt tcaatattta cgtctaatat
    17461 tagtttctta aggttaagtt aatattctat ccttaaaatt tcgctccaaa tggcaaaata
    17521 tacacaacac tctttatagc aaatataagt ggacaggtat tcaatggcgg aaggaaaagc
    17581 aatgtcagaa tttttgcctt tttcgcgacc agcaatgggc gtggaggaac tcgctgcagt
    17641 taaagaggtt ctcgaatccg gttggatcac aaccggtccg aagaatcagg cgcttgagca
    17701 agccttttgc cagttgacgg gaaatcagca tgccatcgcg gtcagttcag ccaccgccgg
    17761 aatgcatatc acgctaatgg cgttggaaat tggcaagggc gatgaagtga ttacgccttc
    17821 cctgacctgg gtttcaaccc tcaatatgat ttccttgctg ggtgcaacgc cggtaatggt
    17881 ggatgtcgac cgcgatacgc tgatggtcac gcctgaagcg atcgaatcag ccattacgcc
    17941 acgcactaaa gccatcattc cggtgcatta tgccggtgcg ccagcagata ttgacaccat
    18001 tcgcgccatt ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc atgccgtcgg
    18061 tacgtattac aaagggcgac atattggcgc aaaaggtacc gctatttttt catttcatgc
    18121 cattaaaaat attacctgtg ctgaaggtgg cctgattgta actgataatg aaaaccttgc
    18181 ccgccagcta cggatgctga aatttcacgg tctgggtgtc gatgcctatg acagacaaac
    18241 ctggggccgt gcaccgcagg ctgaagtctt aacaccgggc tataagtaca atctgaccga
    18301 tattaacgcc gcgattgccc tgacacagtt agtcaaatta gagcacctca acacccgtcg
    18361 gcgcgaaatt gcccagcaat atcagcaagc actggcagct ctcccctttc agccattaag
    18421 ccttcccgcc tggccgcacg ttcacgcctg gcatctgttt attattcgtg tcgatgaaca
    18481 acgttgtggt atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag gcattggtac
    18541 cgggttacat ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt ttcccacgct
    18601 gtcgttaccg aataccgaat ggaatagcga acgcatctgt tcgttgccgc tgttcccgga
    18661 tatgactacc gccgatgccg accgcgtcat cacagccctt cagcaactcg caggacaata
    18721 agccatgttt gaaatccacc ctgttaagaa agtctcggtg gttattcccg tttataacga
    18781 gcaggaaagc ttaccggaat taatcaggcg caccaccaca gcctgtgaat cgttggggaa
    18841 agagtatgag atcctgctga ttgatgacgg cagtagcgat aattccgcgc atatgctggt
    18901 cgaagcctca caagcggaga acagccatat tgtgtctatt ttgcttaacc gcaattacgg
    18961 gcaacattca gcgattatgg cgggattcag tcacgttact ggcgacttaa ttattaccct
    19021 tgatgccgat ctccagaatc cgccagaaga aatcccccgc ctggtggcaa aagccgatga
    19081 aggttacgac gtggtaggga ctgtacgcca gaaccgccag gacagctggt ttcgtaaaac
    19141 agcttcgaag atgattaacc ggcttattca gcgcaccact ggcaaagcga tgggtgacta
    19201 cggttgtatg ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc actgccatga
    19261 acgcagcacc tttatcccga ttctggcgaa tatcttcgcc cgccgtgcaa ttgaaattcc
    19321 agtacatcat gccgagcgtg agtttggtga atccaaatac agttttatgc gcctgattaa
    19381 tttgatgtac gacctggtga cctgccttac cacaacgccg ctacgtatgc tgagtctgct
    19441 cggcagcatt attgcgattg gaggttttag cattgcggtg ctgctggtga ttttacgcct
    19501 gaccttcgga ccacaatggg cggcagaagg cgtctttatg ctatttgccg tgctgtttac
    19561 ttttattggc gctcagttta tcggcatggg attactcggt gaatatatcg gcaggatcta
    19621 caccgatgtc cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc catccagcaa
    19681 ggaaaatgaa taatgaaaac cgtcgttttt gcctaccacg atatgggatg cctcggtatt
    19741 gaagctctgc tggctgccgg ttacgaaatt agcgccattt ttacccatac cgataatccc
    19801 ggtgaaaaag ccttttatga ttcggtggct catctggcgg cggaaagaga cattccggtt
    19861 tatgcgccgg ataacgttaa tcatccgctg tgggtggaac gcattgccca actgtcacca
    19921 gaggtgattt tctcttttta ttatcgccat cttatttgcg acgagatttt tcagctcgct
    19981 cccgcaggtg catttaatct gcacggttcg ctgttaccaa aatatcgtgg tcgcgcgccg
    20041 ctgaactggg tgctggtgaa cggtgaaacg gaaacgggcg ttacattgca ccgaatggta
    20101 aaacgtgccg atgctggggc cattgtggcg caactgcgcg ttgccattgc gccagacgat
    20161 atcgctatta cgctgcatca taaattgtgc catgccgcgc gccagctact ggaacagaca
    20221 ttacccgcca ttaaacacgg taatattctg gaaatcgccc agcgcgaaaa cgaagccacc
    20281 tgttttggtc gcagaacgcc ggatgacagt ttccttgaat ggcataaacc ggcatccgta
    20341 ctgcacaaca tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag ctatgttggc
    20401 aatcagaaat tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa agcacagccg
    20461 gggagcgtga tttctgttgc gccactgctg attgcctgtg gcgatggcgc gctggaaatc
    20521 gtcaccggac aggcgggcga cggcattact atgcagggct cgcaattagc gcagatgctg
    20581 ggcctggtgc aaggttcacg cttgaatagc cagcctgcct gcaccgcccg acgccgtacc
    20641 cgggtactca tcctcggggt gaatggcttt attggcaacc atctgacaga acgcctgctg
    20701 cgcgaagatc attatgaagt ttacggtctg gatattggca gcgatgcgat aagccgtttt
    20761 ctgaatcatc cacattttca ctttgtcgaa ggcgatatca gtattcattc cgaatggatt
    20821 gagtatcacg tcaaaaaatg tgatgtcgtc ttgccgctgg tggcgatagc cacgccgatt
    20881 gaatataccc gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa tctgcgcatt
    20941 atccgctact gcgttaagta ccgtaagcga atcatcttcc cgtcaacttc agaagtttat
    21001 gggatgtgta gcgataaata cttcgatgag gaccattcta atttaatcgt cggcccggtg
    21061 aataaaccac gctggattta ttcggtgtca aaacaattac ttgatcgggt gatctgggcc
    21121 tatggcgaaa aagagggttt acagttcacc ctcttccgcc cgtttaactg gatgggacca
    21181 cgactggata accttaatgc agcgcgaatt ggcagctccc gcgctattac gcaactcatt
    21241 ctcaatctgg tagaaggttc accaattaag ctaattgatg gcggaaaaca aaaacgctgc
    21301 tttactgata tccgggacgg tatcgaggcg ttgtaccgca ttatcgaaaa tgcgggaaat
    21361 cgctgcgacg gcgaaattat caacattggc aatcctgaga acgaagcgag cattgaggaa
    21421 ctgggcgaga tgctgctggc gagcttcgaa aaacatccgc tgcgccatca tttcccaccg
    21481 tttgcgggct ttcgcgttgt cgaaagtagc agctactacg gcaaaggata tcaggacgta
    21541 gagcatcgta aaccgagcat ccgcaatgcc caccgctgcc tggactggga gccgaaaatt
    21601 gatatgcagg aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt tgatcttacg
    21661 gataaaccat catgaccaaa gtaggcttac gcattgatgt cgataccttt cgtggcaccc
    21721 gtgaaggcgt gccgcgtctg ctggaaatct tgagtaagca taatattcag gccagcattt
    21781 ttttcagcgt cggcccggac aatatgggcc gccatctctg gcgactggtg aagccacagt
    21841 ttttgtggaa gatgctgcgc tcaaacgcgg catcgcttta tggctgggat attttactgg
    21901 caggtacggc ctggccaggt aaagagattg gtcatgccaa tgccgatatc attcgtgaag
    21961 cggctaaaca tcacgaagtc ggtctgcacg cctgggatca ccatgcctgg caagcccgta
    22021 gcggtaactg ggatcggcaa acaatgatcg acgatattgc acgcggtctt cgcactctgg
    22081 aagagattat cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt gccgaccaga
    22141 aggtgatcga agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt cgtggggcca
    22201 tgccgttccg tccactgctc gaatcaggaa accctggcac tgcgcaaatt ccggtgacct
    22261 tacccacctg ggatgaagtg attggtcggg atgtgaaagc agaagatttt aacggttggt
    22321 tactcaaccg catcctgcga gataaaggca cgccggttta taccattcat gcagaagttg
    22381 aaggctgcgc ttatcagcat aattttgtgg atctcctcaa acgcgcagct caggaaggcg
    22441 tgacattttg ccctttaagc gaactgttat cagagacgtt gccgctcgga caagttgttc
    22501 gcggaaatat tgccggacgt gaaggctggc tgggttgcca acaaattgcg ggtagtcgct
    22561 gatgaaatcg gtacgttacc ttatcggcat cttcgcgttt attgcctgct attacctgtt
    22621 accgatcagc acgcgtctgc tctggcaacc cgatgaaacg cgttatgcgg aaatcagtcg
    22681 agaaatgctg gcatccggcg actggattgt gccccatctg ttagggctac gttatttcga
    22741 aaaacccatt gccggatact ggattaacag cattgggcaa tggctatttg gcgcgaataa
    22801 ctttggtgtg cgggcaggcg ttatctttgc gaccctgtta actgccgcgc tggtgacctg
    22861 gtttactctg cgcttatggc gcgataaacg tctggctcta ctcgccacag taatttatct
    22921 ctcattgtct atgccatcgg cacttatgcc gtgctcgatc cgtttattgc attctggctg
    22981 gtggcgggaa tgtgcagctt ctggctggca atgcaggcac agacgtggaa aggcaaaagc
    23041 gcaggatttt tactgctggg aatcacctgc ggcatggggg tgatgaccaa aggttttctc
    23101 gcccttgccg tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa acgctggaaa
    23161 gatctcttta tttacggctg gctggcggtt atcagttgcg tactgacggt tctcccctgg
    23221 ggactggcga tagcgcagcg ggagcctgac ttgtggcact attttttctg ggttgagcat
    23281 attcaacgct ttgcactaga tgatgcccaa catagagctc cgttctggta ctacgtgccg
    23341 gtcatcattg ccggtagcct gccgtggctg ggattactcc ccggtgcact gtacacaggc
    23401 tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga gctggacgat aatgccgctg
    23461 ctgtttttct ccgtcgctaa aggcaaattg cccacctata ttctttcttg ctttgcacct
    23521 ctggcaatgc tgttggcgca ttacgctttg ctggcagcaa aaaataatcc tctggcgctg
    23581 cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc cacatttgtg
    23641 atctccccct ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag ctataaagtc
    23701 ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg gctggtacac cttaacaaac
    23761 gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg ggctggcgtt gctggtagga
    23821 ttttcaattc ctgacagagt catggaagga aaacatccgc aattttttgt cgagatgaca
    23881 caagaatcac ttcagccaag ccgctatatt cttactgata gcgtcggtgt tgccgcaggt
    23941 ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg tgagttgaaa
    24001 tacggcctta attatccgga tgcgaaaggg agatttgtca gcggtgatga gttcgcaaac
    24061 tggcttaatc aacatcgtca ggaggggatt attacactcg tgctttcggt tgaccgcgat
    24121 gaagatatca acagtctcgc cattccgccc gcagatgcca tcgttcgtca ggagcgtctg
    24181 gtgctgattc agtatcgtcc caaatgatct ggctaacatt agtctttgcc agcttgctta
    24241 gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgtggcgata aacaaacggc
    24301 gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt gccatggtgc
    24361 tctggctgct ggtcttgcag aacgtaccgg taggcattgc ctacccgatg ttaagtctga
    24421 attttgtctg ggtgacgctg gctgcagtaa aactgtggca cgaaccggta tcgccgcgtc
    24481 actggtgtgg ggtggcgttc attattggtg gcattgtgat cctcgggagt acggtgtaat
    24541 gggcctgata tggggattat tcagcgtcat tattgcctca gttgcgcaat taagcctggg
    24601 ttttgcggca agtcatctgc cgccgatgac gcacttatgg gattttatcg cggctctgtt
    24661 agcttttggc ctggatgccc ggatactgtt actcgggctg ctgggttatc tgctttccgt
    24721 tttttgttgg tataaaacgc tacataaact tgcgctaagc aaagcctatg ccttgctcag
    24781 tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg aagggacttt
    24841 ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc tgattttcct
    24901 gcccacgaca aaacaacgtt actgagtttt ccctgccgct gtacactgcc gttcccactc
    24961 atcggggcta taactgcttg ccgagaggac tttcaccgta tgcagctttt cacgattaat
    25021 acaataaagc gcattctgca aaggcgagag taaatccccc actttcacgg cgaagtcact
    25081 cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa gtacatgtct
    25141 gttatcttgt ttattgcagc acgatttttt gtgccagcca ttccattgca ttatcctgtt
    25201 tgctaagagt tttcacatca atttacagat taacagataa actctttatc aacggcaatg
    25261 gaatggcaga aatgagaagt gaaacggagg gttaattatt gctgacgttg cacccactct
    25321 tttagcgcct gacgtgaaat tttaataccg ccgtttttca gctccggcgg taaagtcagc
    25381 cagcgcaccg gttgttgaaa acgagccagc ttatctttca cccattcgcc aagatcaacg
    25441 gtttgctggt catactccac caccgctacc ggccgatgac caaactcctt gtcggcaacg
    25501 gaaacgataa acacctgcaa aaccgcagga tgtgcagcaa taacacgctc gacttcttcc
    25561 ggctgaatac cctcaccgcc actgaagaac agattgtcta aacgtccgac aatggtcagc
    25621 ttgccattat gcatctcacc gcgatcgcgc gtagcgtacc agccttcgtc attaaccagt
    25681 gaaaccagtt gcccgttacg ccagtaacct tctgccatac tggcagcccg cagccacact
    25741 tcattattaa cgattttcac ttcccgaccc ggcagcggca aaccaacgtc tgccaggccg
    25801 tcggcttctt tcgcacatac cgtggaggca aactcggtca gaccatagcc gcaaaagcaa
    25861 cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg ggatagccgc gccgccaagt
    25921 aacaccgctt tcagggaaac ggaactgcgg ttaaccagca aacgccagag ttgtgttggc
    25981 accagtgaag cgtgagtaca gcctgccagc atttgctcca atggctgttt atcacgcacc
    26041 gtcatccgcg caccagcgta taaccagcgc cacataattc cctgaccgga gacgtgaaac
    26101 agcggtaaag agagcaacca gtcatcgtga tcgccaaacg gaatcagcga taacacacct
    26161 tgcgcactgg caagatgggc ctgataagta tggacagcgg ctttcggcaa accggtagaa
    26221 cccgaggtca acgtcattga gcacagacgc gtcggctgcc acgcagcggc atgtacgcct
    26281 tcaaccagct gaatgtgcag cgacgttaat gccggaaacg tgttttcccc atccggcacc
    26341 agcgcaaatt gcagcgtcag attgggcagc aattcttcaa gcaacggttg cggcagctga
    26401 gggttcacgg gcaacacccg cgccccgcat tgcagtaacg ccagccaggc gagcagcgtt
    26461 tgcggcgtat tccacgcccg caacatcacg ccgctgccct caaccacacc ctgcgccgca
    26521 aagcccgtag ctaactcatc gacgcgagca caaagctcgc gccagttgag ttgctcgtca
    26581 ttaagacgta aggcgatggc ttctccccgc acttgccgcc agtgacgcca cggccagtca
    26641 gagaagatca taacaactgc tccagtacat cagcgtccac taacggcagt gggctccccg
    26701 gccagcgacg aacctgctgc gcctgcatca gatccagcgt gtccagtccc ggaatggtgt
    26761 ctggcgttaa ccaggcagca atccgcgcca gttgcgttag acctaagctc gactcaatgg
    26821 aagaactgat caccgccgtc agccccagcg catgcgccgc ctgtacctgc tcgcgtactt
    26881 tttccagact gcccgtgagc gtgggtttga taactaccgc gcgcacgccc tcttcagcca
    26941 caaaggcaaa atccggctcg cgcaggcttt catcccaggc aatggcaatg ccggtttcac
    27001 gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc ttcgagaaac gcgatgcggt
    27061 ggcgataatc cgggttaaca tatttggtaa actgctgacc tttcagcggt gtccaggcgc
    27121 gatttgcgtc aagacgcaaa tgcagatccg gaattgcctc cagcaacaga ttcaccacca
    27181 tgccgtcgcg caccgcttcg tacaatccca ctttgacctt cgccactttc tcgcccggca
    27241 tatcggcaag tttgaggatc agatcgtccg gatcgccatt acacagcggt gccgcacggt
    27301 agttggctgc ttgcggcaac gtatctgtca gttctgccaa tgcacagctt acgccaaagg
    27361 ccacggaagg catctgcggt agctcgcaat cgcctgccag ccagttattt acccaggcaa
    27421 gcagcacgct ttgcgcctct tcccagtttt cctggctgaa gcccggcagt ggggagatct
    27481 ccccccaccc ttcgcgctcg ccctcacgca ggcaaacgta cagcccgtcg cgggttttta
    27541 accgcctgtc gcgcagaacc acccccgcgt ccatggggat ctgccagcgg tatacctgcg
    27601 cgctacgcat tacggattcc gtttaaattt gctgaaatca ggctgacgtt tctggttgaa
    27661 agcgttgcga ccttcctgac cttcttccgt catgtagaac agcatggtgg cgttgcccgc
    27721 cagctcctgc agccccgcct gcccgtcaca gtcggcgttc agcgcagctt tcaggcagcg
    27781 cagcgccatc gggctgtttt gcagcatttc gcggcaccaa cggacggttt ctttttccag
    27841 atccgccagc ggtaccacgg tgttgacaag gcccatatcc agcgcctgtt ttgcgtcgta
    27901 ctgacggcac aggaaccaga tttctcgcgc ttttttctgc ccgacgatac gagccatgta
    27961 ggaagcgccc cagccgccgt cgaaggaacc gactttcggg ccagtctgac cgaagatggc
    28021 attatctgct gcgatagtca ggtcgcacat catgtgcaga acgtgaccac cgccgatgga
    28081 gtagccagcc accatcgcga caaccggttt cggacaggta cggatctgac gctggaagtc
    28141 cagcacgttc aggtgatgta cgccggaatc atctttatag ccgccgtaat caccacgcac
    28201 tttctggtca ccaccggagc agaacgcttt atcgcctgcg ccagtcagaa tgatcacgcc
    28261 gatattgtcg tcataacgcg catctgccag tgcctggatc atctctttta ccgtcagagg
    28321 acggaaggca ttgcgcacct gcggacgatt aatggtgatt tttgcgatac cgtcggtgga
    28381 tttttcataa caaatgtcct cgaaaccttc ggagcagtcg tgccattcaa ccggtgcgta
    28441 aggcattgct tcatcaggat aaatcatatt gtgtccttta gtcaaaaacg caagatctgc
    28501 gccagacttg cgattacgcc agcgggattt tcccgatgcg cgttatgtcc ggcgcgagga
    28561 atgacatggc agtcggcagc cagttccgcc gccagggcgc ggaatttgct gtcacgttca
    28621 ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt tagcacgtaa atcaggctgg
    28681 acggcgagag aagtcgcctc cagcatggcg gcaagggttg cgccattatt gttgctgcgc
    28741 agcgccacca gctcccggcg ttgatcgtca ttgagcgagg caaaaacagg ctgttgatac
    28801 cagtcggcaa ataccgccgt taacggttct gtgcgaaaac gctgcgccca ttggcgatcg
    28861 gaacgctgac gttccgcacg ttgttcagca ttttgcagcc ccggatgccc gccttcgata
    28921 ataaccccac aaagccccgc caacccctgg caagccgcca tcatcgccac ccgtccgcca
    28981 agcgagtacc ccaccagcca gaagtcaagg atgttgtaac taaccaaggt tttacgcagt
    29041 aagtcggtga catcatcaaa tccatcgacg ctaatcgccg ccgaaccacc atgacctggg
    29101 agatcaacat acaaccgtga gtagtcggca aacgcctcgc ccacttcttg ccattcgtgg
    29161 caatcgccgg aaaaaccgtg caaaaacacc agccagggta aacctggttt tccgtgtttt
    29221 gcctgcgcgt gcaggatcat aaatggctta cctgcgccag aagttgctgg agcgtttgcg
    29281 caccatcggt gtcgttaacc accatttcaa tcaccgtggt ggttggcgtg cgccaggcgt
    29341 cggcaaatgc cgtttcaagt tcctgccagt tttgcggacg atgatatttc agctcgaaca
    29401 tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc tcacgctcgc
    29461 tttgcggcgt tggcaacagc gagaaaattt gcccgccgtt gttgttcacc acaattaata
    29521 ccagcggcgc agaaacctga cgcaataacg ccagcgcgtt gagatcgtaa agtgcggaga
    29581 gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg ctgaacgcct gcggcggtcg
    29641 aaagcagtcc gtcgataccg ctggcccctc ggttgctgta caccgggtaa cctgccggaa
    29701 gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt accaacaaac aattgcccct
    29761 gttcaggcag atagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac gcatcacggc
    29821 gggcaataac cgcctgcatt gcctgttccg ccaggcgcgg gatttcaacg caccagggct
    29881 ggcgtttttc tgccggatgc agctccagcc agtcggcaat attgccaatt aagcgacgtc
    29941 cgcgatggtg tgccggatca agtcgccctt caatgtcatc aacaatccag tactcttctg
    30001 gttcacagct tgcctgccat tgcaggagcc gtttccccgt caggctgctt cccagttgca
    30061 ccacaatttg tgcctgctgc agctcgctgg tcgctttggc attgcctaac caaagatcgg
    30121 cacacggcag cggctgcccg gtttgtgaca gcacatcgcc aatcagcggc cagccaagag
    30181 tttgcgccca cagggcaact tttttgccct cttccgcact catgcgcccg gcaaccacca
    30241 cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt ttcactttcc agacgaggcg
    30301 cttcacgcag ccacggtttg tcgtcctgcc accagtcacc cagacgctgt tgccagctaa
    30361 gcccggtatc gtccatttcg ccatacagcg gttcagcaaa cgggcagttg atatggactc
    30421 cccccgcatg aagcgtaccg agagcgtggt cgatggtaga aaccagccaa cgtgcgggga
    30481 tatcctgggt cgggcgcggc aatgaaatac tgtgcgtggg gtgagaggcg aacattcccg
    30541 gctggcgaat tgcctgattc gcgccgcagt caattagctc cggcgggcga tcggcggtta
    30601 agagaatcag tttttctccg gttaacccgg cttcaatcag tgccggatag agatttgcca
    30661 ccgccgtgcc ggaggtcaca atcaccgcca ccggctgctt gctgactttc gccagcccca
    30721 gcgccagatg ccccaaccca cgctcatcga aatgggtgtg gtgaatgaat gcggaattct
    30781 ccgccgccgc taacgttaac ggtgtagaac gcgagcctgg ggcgatacag atgtgtctga
    30841 cgccgtgacg cgttaatgct tccagaatga ccgccgccca gcgtcggtta aatgcgctta
    30901 ctgacatgag tttgtccggt atcaatattg cggctaagta taaggagcta caaaaataga
    30961 ttattgatat gaatcggtaa tgatgcgact cattactatt ccatttgtaa taaagtacgc
    31021 agccctgccg ctttgttgtc gatttcctgc cactcttgct cggggtcgga accacggaca
    31081 atgcccgcgc cagcatataa tcgcacgaca ttgccgctaa tttttgctga gcgcagggaa
    31141 acgcagaatt cgctttgttg taatgagaga tagcccgcag aaccggcgta ccattctcgg
    31201 gtgaacggtt cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa gccagcaact
    31261 gctgccgttg gctgcaactg atgtaaacag atcacatcat ccgctttgtt gagtgaagtc
    31321 cagatacagc ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca
    31381 tccagcgtct gggtatcggc ctgtaatcgt tggcagatat cttccaccac cagcatgttc
    31441 tcgcgctggt ttttatcatc cgccatcagc ctctctccta actgctgcgc ctgcttatca
    31501 tcaggatgat ttgctactgt tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc
    31561 cgccataacc gttccggtga agagccaaga aaagcatttt cgccatcaaa ggccatgtaa
    31621 aaatggtagc aattcagatt cagtcgacga ctggcagcca tcatcgccgc cgcgttgacc
    31681 ggacttgcga aatgcaggtc agttgcccga gcgagcacca ctttgtcgag ctcacctttg
    31741 gcgatggttt tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt
    31801 tgttctcgcg tggtggttaa atgtaaccca ggcaagggct tgatactcac cagtgtggcg
    31861 ataaattctt ttgcctgaat cgcatcgtgc tgaagggagc tttcgctgaa tagcgtcaac
    31921 cgcagcgtgg ctttaccgcc acagcgtcgc cattccaggc ggggtaaaag taaattgccc
    31981 tgcgacgggt caaaagcatt cagcccccaa atgcgtaagt cggcgtgttc cgggtgctgg
    32041 cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc caggacggca
    32101 gcttcttcat caccattacg ttgttgccag tagaattgcg ggtacgtttg ctgactggcc
    32161 agccagctca aggcatcaaa agcgtctttg agagggaaag gaatatcgat aacccgaatg
    32221 ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta gattttccag cgccgtagta
    32281 agtgattgca cgcggacctc tccctgttaa aagcctcaca ttatacgggg tactacaaaa
    32341 aaaatgcagt accccggtgt agggaggttt taacggcgtg ccagcaacag tcctagtacc
    32401 agcccgacgg ccgcgcccac accaattcct tgccagggtt tttcgtggac gtagtcatca
    32461 gcacgataaa cagcctgctt cgcccgatag taataactgt ctgaagcctg gctaacccgt
    32521 tttttcacat catccagcgc tttttctgca cgcgctttca gctcaacata tttctgatcg
    32581 gcgggctcgc cagaggagcg gagcacctct tccagtgttt cactaagcag cgtcaggtcg
    32641 tcatcgatac gtgtatcacc aaactgatta gacattctcg ttctccattt gcgtaaaacc
    32701 tgtcactaac tatagacaag agttgttgat tacgcctgaa ttacctcgcg tgccatccca
    32761 atgtgtggga tacaatcttc ttcatagacc tccgtcacag ggataaaacc gaaactctgg
    32821 tagaagtttt gcagatgcgc ctgcgccccg aggtaaacag gcttatcagg ccagtgatgc
    32881 gtacatgttt cgagtgtttt actcatcagc tgctgcccca ctttttcacc acgcaacgct
    32941 tcactgacaa tcacccgacc tataacgacc ggctcaagat catcatcact tttcagaatc
    33001 ctcgcatacg ccaccagttc atcatttttc caaccgagaa tatggcgatt atcccccgtc
    33061 aaatcatcac catcgatatc ctgataaggg caattctgct cgacaacaaa caccgcgcaa
    33121 cgcagttgta ataaggcata aagctgggaa accgataatt cagagtgatg cagatcttgc
    33181 cattcaatca taatgctctc cttttagatg tatgccttat actagccgga atattttttg
    33241 aaacgtgatg aactcatgga attaattttt ttaggtactt cagccggtgt gccaacccgc
    33301 acgcgcaatg tcacggcaat attgcttaac ctgcaacatc cgacccagag cggactttgg
    33361 ttgtttgact gcggtgaagg cacccagcat cagctactgc ataccgcctt taaccctgga
    33421 aaactggaca agatttttat cagtcacctt catggcgatc atctttttgg tttacccggc
    33481 ttgctgtgca gtcgttctat gtcaggcatt atccaaccct taacgattta tggtccgcaa
    33541 ggtatccgtg aatttgtgga aaccgcgctg cggattagcg gctcatggac cgattatccg
    33601 ctggaaattg tcgaaattgg cgctggcgaa attctcgatg atggcctgcg caaagtaacc
    33661 gcttatccgc tggaacaccc actggaatgt tatggctatc gtattgaaga acatgataaa
    33721 ccgggtgcat taaatgccca ggcattaaaa gctgctggcg tgccgcctgg cccactgttt
    33781 caggaattaa aagcgggcaa aacaatcacg ctggaagatg gaaggcagat taacggcgca
    33841 gattacttag ctgctccagt gccaggtaaa gcgctcgcta ttttcggcga taccggcccc
    33901 tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg
    33961 gatataacca tggaagccaa agccaatagt cgcggccata gctctacacg ccaggctgcg
    34021 acactagccc gtgaggctgg agtcggcaag ctaatcatta cccacgtcag ctcgcgctat
    34081 gatgacaaag gttgtcaaca cctgttacgt gaatgcaggt caattttccc ggcgactgaa
    34141 ctggcgaatg atttcaccgt gtttaacgtt taactctgct tattattaaa aacagggtga
    34201 aacttgccct gttatagcaa cccgcgctac atatatccac ccacacaggc atattgagcg
    34261 atttttgata tttattcatc tccttacgca ctatattcag agcaataagt tgcctctttt
    34321 catcttgatt cagcacaacc tttgcaacta aaaggagccc atgatggtta cagttgtcag
    34381 caattattgt caattatctc aaacccaact cagtcagaca tttgcagaaa aatttactgt
    34441 gaccgaggaa ttactgcagt ctttaaaaaa aacagcgtta tccggagatg aagaaagtat
    34501 tgagttactg cataatattg cgttaggtta tgatgaattt ggaaaaaaag ctgaagatat
    34561 tctttaccat attgttagaa acccaacaaa tgagacctat cgattatccg acttattaaa
    34621 aatgcctgtt taaaattata taatttggca cataccgcaa ccaactccca ccttaaacca
    34681 actggtccgg ataattcaga tgttctcctg ttcaaaaagc tattctcccc ttcgaaatta
    34741 atgacaatta tcggtaatga aattcctctt atatctgaaa aacagtcgct ttcaaaggtg
    34801 cttttaaatg atgagaataa tgaactgagt gatggtacaa acttctggga taaaaatcgt
    34861 caattaacca cagatgaaat agcttgctat cttcagaaga tcgccgctaa tgcaaaaaat
    34921 actcaagtca attatcctac tggtctctac gtcccctact ccaccagaac tcacctggaa
    34981 gacgctctca atgaaaatat taagagcgat ccatcatggc cgaatgaagt ccagt
//
LOCUS       DADWTH010000036        34281 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.036, whole genome shotgun sequence.
ACCESSION   DADWTH010000036 DADWTH010000000
VERSION     DADWTH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.036"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(93..2189)
                     /locus_tag="JNY02_001823"
     CDS             complement(93..2189)
                     /locus_tag="JNY02_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9433377.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYARELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2191..2442)
                     /locus_tag="JNY02_001824"
     CDS             complement(2191..2442)
                     /locus_tag="JNY02_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9433378.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2595..3158
                     /locus_tag="JNY02_001825"
     CDS             2595..3158
                     /locus_tag="JNY02_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433379.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3434..3856)
                     /locus_tag="JNY02_001826"
                     /pseudo
     CDS             complement(3434..3856)
                     /locus_tag="JNY02_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(3856..4449)
                     /locus_tag="JNY02_001827"
     CDS             complement(3856..4449)
                     /locus_tag="JNY02_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBB9433380.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4596..5003
                     /locus_tag="JNY02_001828"
     CDS             4596..5003
                     /locus_tag="JNY02_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB9433381.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5123..6115)
                     /gene="rpoS"
                     /locus_tag="JNY02_001829"
     CDS             complement(5123..6115)
                     /gene="rpoS"
                     /locus_tag="JNY02_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBB9433382.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6178..7317)
                     /gene="nlpD"
                     /locus_tag="JNY02_001830"
     CDS             complement(6178..7317)
                     /gene="nlpD"
                     /locus_tag="JNY02_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBB9433383.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGAQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7457..8083)
                     /gene="pcm"
                     /locus_tag="JNY02_001831"
     CDS             complement(7457..8083)
                     /gene="pcm"
                     /locus_tag="JNY02_001831"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBB9433384.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8077..8838)
                     /gene="surE"
                     /locus_tag="JNY02_001832"
     CDS             complement(8077..8838)
                     /gene="surE"
                     /locus_tag="JNY02_001832"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBB9433385.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8819..9868)
                     /gene="truD"
                     /locus_tag="JNY02_001833"
     CDS             complement(8819..9868)
                     /gene="truD"
                     /locus_tag="JNY02_001833"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBB9433386.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9865..10344)
                     /gene="ispF"
                     /locus_tag="JNY02_001834"
     CDS             complement(9865..10344)
                     /gene="ispF"
                     /locus_tag="JNY02_001834"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBB9433387.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10344..11054)
                     /gene="ispD"
                     /locus_tag="JNY02_001835"
     CDS             complement(10344..11054)
                     /gene="ispD"
                     /locus_tag="JNY02_001835"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBB9433388.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11073..11384)
                     /gene="ftsB"
                     /locus_tag="JNY02_001836"
     CDS             complement(11073..11384)
                     /gene="ftsB"
                     /locus_tag="JNY02_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBB9433389.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11578..11901)
                     /locus_tag="JNY02_001837"
     CDS             complement(11578..11901)
                     /locus_tag="JNY02_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBB9433390.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11951..12556)
                     /gene="cysC"
                     /locus_tag="JNY02_001838"
     CDS             complement(11951..12556)
                     /gene="cysC"
                     /locus_tag="JNY02_001838"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBB9433391.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12556..13983)
                     /gene="cysN"
                     /locus_tag="JNY02_001839"
     CDS             complement(12556..13983)
                     /gene="cysN"
                     /locus_tag="JNY02_001839"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBB9433392.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13985..14893)
                     /gene="cysD"
                     /locus_tag="JNY02_001840"
     CDS             complement(13985..14893)
                     /gene="cysD"
                     /locus_tag="JNY02_001840"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBB9433393.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15145..16182
                     /gene="iap"
                     /locus_tag="JNY02_001841"
     CDS             15145..16182
                     /gene="iap"
                     /locus_tag="JNY02_001841"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBB9433394.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16328..17419)
                     /locus_tag="JNY02_001842"
                     /pseudo
     CDS             complement(16328..17419)
                     /locus_tag="JNY02_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(17613..17765)
                     /locus_tag="JNY02_001843"
     CDS             complement(17613..17765)
                     /locus_tag="JNY02_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBB9433395.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18029..18763)
                     /gene="cysH"
                     /locus_tag="JNY02_001844"
     CDS             complement(18029..18763)
                     /gene="cysH"
                     /locus_tag="JNY02_001844"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBB9433396.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(18837..20549)
                     /gene="cysI"
                     /locus_tag="JNY02_001845"
     CDS             complement(18837..20549)
                     /gene="cysI"
                     /locus_tag="JNY02_001845"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBB9433397.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20549..22348)
                     /gene="cysJ"
                     /locus_tag="JNY02_001846"
     CDS             complement(20549..22348)
                     /gene="cysJ"
                     /locus_tag="JNY02_001846"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBB9433398.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22667..23029
                     /gene="queD"
                     /locus_tag="JNY02_001847"
     CDS             22667..23029
                     /gene="queD"
                     /locus_tag="JNY02_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBB9433399.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            23107..24378
                     /locus_tag="JNY02_001848"
     CDS             23107..24378
                     /locus_tag="JNY02_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB9433400.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPSWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24369..24629
                     /locus_tag="JNY02_001849"
     CDS             24369..24629
                     /locus_tag="JNY02_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBB9433401.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24646..25221
                     /locus_tag="JNY02_001850"
     CDS             24646..25221
                     /locus_tag="JNY02_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBB9433402.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25369..26229)
                     /locus_tag="JNY02_001851"
     CDS             complement(25369..26229)
                     /locus_tag="JNY02_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBB9433403.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(26226..27005)
                     /locus_tag="JNY02_001852"
     CDS             complement(26226..27005)
                     /locus_tag="JNY02_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBB9433404.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26983..28392)
                     /locus_tag="JNY02_001853"
     CDS             complement(26983..28392)
                     /locus_tag="JNY02_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9433405.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28414..29868)
                     /locus_tag="JNY02_001854"
                     /pseudo
     CDS             complement(28414..29868)
                     /locus_tag="JNY02_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29938..30724)
                     /locus_tag="JNY02_001855"
                     /pseudo
     CDS             complement(29938..30724)
                     /locus_tag="JNY02_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31042..32319
                     /locus_tag="JNY02_001856"
     CDS             31042..32319
                     /locus_tag="JNY02_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9433406.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     HGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32346..33824
                     /locus_tag="JNY02_001857"
     CDS             32346..33824
                     /locus_tag="JNY02_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBB9433407.1"
                     /translation="MSKKYIIGIDGGSQSTKVVIYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHILFDVQMPGTVLGHITPQGALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         8283 a         9076 c         8422 g         8500 t
ORIGIN      
        1 tcatcaatat cagtcatagt atttcctcgg ttatttcaaa tcagctttgt ttcctttatt
       61 taacagtccg ttcccgcgga ctgttttttt attcaggcca gcggatcatc ttcttcctgc
      121 atggcaaaaa gtttttgcat ccccagccgg aaatcgcgtt caaattcttc gtcatgctct
      181 tcttcatatt ccggttgctg aactgccacc acattaccaa ccgggcgata aacattactc
      241 agccagggtt cagcctgttt gtgttccaga atctgaatac cttcatcttc agcatcacga
      301 attttttcct cagcgcgttt gcgcattcct ttaatacgct gctgttgttt gtgatattca
      361 gcactgacag caaaggctgc gcgtttattg ccgttccata tcgcctcaca gataaagctg
      421 ccgtccttgc gacgtaccgt aattcgctcg gcgtcatgaa tgtcataact gataagcact
      481 ttgcgaccgt gttcgtcacg caactcccgc gcgtagtaaa tattgttcag ccactgaatt
      541 tcgcagcgtc ttaccggtcg ttccaccatt gggcggaaca tgtcgcgcag ctcaagatca
      601 ctcagccact caatttcggt gtcttcttct gccaggcgtt tttttctgaa ctctgtcggg
      661 ctgtaatgcc gtccgttcgg tttcatcggc agctcagaat gcgggcggtt gttgtaccat
      721 tccacgcctt cacgaatagc ctcaataagc tccgcccacg atggcagctc gcgcattgcc
      781 gctttctggc ggtcgttcag aggcttgtcc tgttgcaggg cgttaaacgc tgaacgcaga
      841 tcacggttaa gtacacgcaa cgattcccgg tcagcaccct tgccaaaata ggtgcgatac
      901 ctcctggcta tacgcatcgg cagtgtccgg ttcaggcgtt caataatgcc gcgtccctgt
      961 ggattaccgg caataccggt agggtgttta atccccaggc gtggcagaat acccacgacc
     1021 tctttgtcca gcatgtcagc cgtttcaccg gaaccgttat ccgagtaata caggaacggt
     1081 tttccgtggt tgcgaatgcc gtgctgtatc gcaccggcaa cggcgaacac gttttcggca
     1141 aggtcaagac tccagccaac aacaaaccgt gttccaccat cgatcacaaa cgtgacttcc
     1201 ggtgagaatg gacgcccgtg gatcggatgc gcacatttca gcttcatacc gtgaccgtca
     1261 ccaatccaga cataattcac cggcatagct gtccagtcgc ggcgggtgaa tccttcaagc
     1321 tgacggtatt cactgccggt aacgcgccct ttttgtttca ccacttccgg cagttttttc
     1381 atggcatacc ggacaacatc ataggaaggc atcacctcca gcatgtacgg ttcgtccgca
     1441 tggcgacgct gccattctgc gacaaaatcc tcgtaggctt ctgacatggg tctgccgttc
     1501 acctggcgat actgcgccag aaattcaggc agccagctga tttcttccgg ttttatttcg
     1561 tcgcgttttc ccggtgccag taacagcagc cgttcggcgg cgttctgcga ctggttgtat
     1621 ttcagaaccc actggtaaag tggatcgcgg ctgatagtac gattagcgcc acgttttgca
     1681 ttggcgttaa ctgcggcctg ttgcaggcgc tccggcagct cacctttacg ggctttttca
     1741 acaagaaagt taatggcttt cagtcgcccg aggttaccgg cgctttccag cttcagcact
     1801 tccaccacca gcgcagctct tgcttcagat acccagcgct ggttttcggt cagcgcattc
     1861 aggcgttctt ccatcagttg tggagaaccg cgatatgctt cgattgcctg gacagcgtca
     1921 ggattgcgtg tttttctggt aacagctttc acttcagaaa cattgttctc tgtagccacc
     1981 agctgttcca catagcgttc acgcagtgcc tttcgtgtta tttcaggcag acaatcaatg
     2041 tggtactcaa tggcttttgt gccttcccga cggcgactaa cgtcaccaag atcacgagaa
     2101 aaacgctgta acgcctgtcg gatgcctttt gtggtggctg gcattcctgg tacgccgagc
     2161 aggtcattta cggtaacgta cgactgcata tcacgacgcc ttcctgataa atccatcgtt
     2221 acgataccgg ctgggccaga tgatttctgg cggtacacct atagcgtcag aaattacgcg
     2281 ttcgtatttt ttgtttggtg tgcgaactac cgctttcagt gaatcctgag cataaccagc
     2341 atggagtgaa agctcccgga aggaagacca cccgttatct ttgagcgcca gctgaatctt
     2401 cagcgaaggc caatcgcggt cactatcttc tctttgtctc atctttcctt taacctacaa
     2461 aataacccgc gcggatatcc gtatggataa agattaagat accaaagatt ccctataaag
     2521 ggttctttgg tgcttttatg gtttattttt taatttcatt atttatcaat gaattagata
     2581 gaaaggaacc aagaatgaga aaagcacccc aaggttcttt tcctgccgat ggaaaagaac
     2641 cgattatcga aagaatcttt aggttagtag aacgataccc ttcaagaaat gcggctgctc
     2701 gggcatgggg gatcaatgaa ggcactttaa aaaattatta caacaggcgg gattacgcac
     2761 cgataccacg cccgcatcag ttaaaaaaga tagcagaaag cgaaaatgtt accttggaat
     2821 ggctcatgac gggagagggt gatgttgaac taaaagattc caaagaaccc aaaaaagaga
     2881 atgcggataa tgttgatgct caaattctaa tgttcttgtc atttcttaaa ccacaagaaa
     2941 aacaacaagt agctgatgtt cttgggagaa aaggagcgga gcaattactc attcttctgg
     3001 atgagcacat ccaagagttg cacgctctag ttggagttcg tagagccata gcgttaagcc
     3061 ttggtaattt gccagacgaa aaggttagag agatttacgc gttgtccgaa gctaagggcg
     3121 atcacttaaa tctacctcag aaagcagcca gcgcataacc cagtgcaagt gcctagctgt
     3181 gctaaaatta cattggtttt ggcgcttgca cccatcagcg ctataataaa cacctgaatc
     3241 gtttttgaac ggtctttaaa ggctattgta agaacctgtg agttatcttg aatgttttat
     3301 tgatatcgaa ccgtttaaca tttttggact cgctttatct tgaaaacctt gagttcctaa
     3361 cttgttgatt ccaatctatt cccgtctttt ttaatctctt cccggttatc ttgaataaat
     3421 tacctagtca tacgagatag aaaccgccat cgccatcatt caggcgaaac agcggcagaa
     3481 tatcgaacag gttgatctct tcgccatcga cggtgttctg cgcccaggct ggattggcgc
     3541 ggcgctccgg ggcaatcggg aagttatccc agcggcggat aaactcatca atctgctttt
     3601 tgaccggggt gtttggtggc aggccaaggg aaatcgcgtg gttctgccag gaaccgatgg
     3661 tattcatcgc cacgcgggca tcggtaaagc cgcgaatatt atcaaaccac agcgcgggcg
     3721 cgccgtcgcc gatacgcccg gtggcgttag ctgctgctgc cagatccggc tcagcgttca
     3781 cttcttcgct gattttcagt aactggccgt ggtcatcaag cgcctgtaaa aagctgcgta
     3841 aatcatcaaa tgccattatt cattctcctg agaaaaattc cgggcctgcg gcaatccttg
     3901 ccagcgcctg gcgtggggat gttcgaggcc aaattgatcc agcacgcggg ctaccacatg
     3961 gtggacaatg tcatctaccg tttcgggatg gttataaaag gcaggcatcg gcggcaccat
     4021 cgccacgccc atgcgtgaaa gtgcgagcat attttcgaga tggatggtgc taagcggcat
     4081 ttcacgcggc accagcacca gtttgcggcc ttctttgagc acgacgtccg ccgcgcgccc
     4141 taccaggcca tcggcgtaac cagcgcggat accggcgagc gttttcatac tgcacggaat
     4201 aacgatcatg ccgtcagtac gaaaagaacc ggatgagatg atcgccgcct gatccgccgg
     4261 gttatggctg aagtctgcga gggcagcaac atcgcgagcg ctgtaaggcg tttccagttc
     4321 aatgatggtt ttcgcccact tcgacatcac cagatgagtc tcgacattcg gcatctcacg
     4381 cagcgcttgc agtaatgcca caccaagagg cgcaccggta gcccctgtca tcccgacgat
     4441 cagtttcatt gcttattctc ttaattattc gcatacgaac gttattatta acatactcct
     4501 tcatttccac agcggcaagg attgaaaagt aacaacatct tcttcgtcac taaaaaaccg
     4561 ctatgatagc gacagagaat tagcccggag tttacatggc gttacgaaat aaagcgtttc
     4621 atcagttacg acagcttttt cagcagcaca cggctcgctg gcagcacgag ttacctgacc
     4681 tgaccaaacc acagtacgcg gtgatgcgcg ccattgctga taaacctggt attgaacagg
     4741 tggcactgat agaagcagca gtcagcacca aagcaacgct agcagaaatg ttggcaagaa
     4801 tggaaaatcg tggcttagtc agacgtgaac atgacgtcgc tgacaagcgg cgacgctttg
     4861 tctggctgac tgccgaagga gaaaagctac ttgctgcggc gatacctatt ggtgacagcg
     4921 tggatgagga atttttgggg cgtttgagtg gagaagagca ggagctgttt atgcagttgg
     4981 tgcgcaagat gatgagcaca tagaatgctg attgccgggt aggacgctga cgtgtcttat
     5041 ccaggcgaca aaaacacaag cttcccaaac aaaagaaaaa ggccagcctc gcttgagact
     5101 ggcctttctg acagatgctt acttactcgc ggaacagcgc ttcgatattc agcccctgcg
     5161 tttgcaggat ttcgcgcaaa cggcgcaggc cttcaacctg aatctggcga acacgttcac
     5221 gggtgaggcc aatttcacga cctacatctt ccagtgttgc cgcttcgtac cccagcaaac
     5281 cgaatcgacg tgccagtact tcacgctgtt tggcgttcag ctcgaacagc catttgacga
     5341 tgctctgctt catatcgtca tcttgcgtgg tatcttccgg accgttctct ttttcatcgg
     5401 ccaggatgtc cagcaacgct ttttcggaat caccacccag cggggtgtct accgaggtaa
     5461 tgcgctcgtt aagacgaagc atacggctga cgtcatcaac tggcttatcc agttgctctg
     5521 cgatctcttc cgcacttggt tcatggtcca gcttatggga caactcacgt gcggttcgca
     5581 ggtaaacgtt cagctccttt acgatgtgaa tcggcaaacg aatagtacgg gtttggttca
     5641 taatcgcccg ttcaatcgtc tggcgaatcc accaggttgc gtatgttgag aagcggaaac
     5701 cacgttccgg gtcaaacttc tctaccgcgc ggatcagccc caagttgccc tcttcgataa
     5761 ggtccagcaa cgccagacca cgattgccat aacggcgggc aatttttacc accagacgca
     5821 agttactctc gatcatccgg cggcgagagg cgacatctcc acgcagtgcg cgacgcgcaa
     5881 aataaacttc ttcttcggcc gttaacagtg gtgaataacc aatctcacca aggtaaagct
     5941 gagtcgcgtc caacacacgc tgtgtggctc cctgcgataa cagttcctct tcggccaaat
     6001 cgttatcact gggttcctct tctactaagg ccttttcgtc aaaaacctca actccgttct
     6061 catcaaattc cgcatcttca tttaaatcat gaactttcag cgtattctga ctcataaggt
     6121 ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa agcctggttc cgccgattta
     6181 tcgctgcggc aaataacgca gcgggtttac ggatttcccc ttgtaacgaa tttcaaaatg
     6241 caagcgtgtt gaactggttc cggtgctacc catggttgct attttttgcc ccgccttcac
     6301 ttcttgttgt tcccggacca gcattgtgtc gttatgggcg taggcactca ggtaatcatc
     6361 attatgtttg atgataatca gattaccgta gccgcgcagc gcgttaccag cataaacaac
     6421 gcggccatct gcggtcgcga taattgcctg tcctttgctg cctgcgatat caatcccctt
     6481 gttgcccccc tcagaagcgc caaaggtttc gatcactttg ccctcagtcg gccagcgcca
     6541 ggtggagata ggcgtactgg ttgatgtact gctgacagtc ggctcggttg tgcttgctgt
     6601 tggtaccgtt acaggcgctg tgaccgtggt cgcagttggc ttgttgttcg gcaacatttt
     6661 gttagcactc tgtgcacccg aagactcaga atacgtaatt gtcggttgcg acgcaacagc
     6721 aacggtggaa ttttgtgcag gcttgatcac aactccttgc tctgctgcgt cggcctgggt
     6781 aatagcattt ccgccagtga ttggcgtacc ggaagcatta cccacctgca aggtctgacc
     6841 aacgttcagc gcgtatggtg cctgaatatt gttgcgctga gcaaggtcac ggaaatcgtt
     6901 gccagtaatc caggcgatat agaaaagtgt gtcacctttt ttcacggtat aggtactgcc
     6961 gctataactg cctttcggaa tgttcccata ctgacggtta tagacgatgc gtccgttttc
     7021 catctgtacc ggctgctgag ctactggctg cactggctgg atttgcggtt gttgagtagc
     7081 ctgaatttgt ggctgctgta ccggctgaat ttgcggttgc tgcgctgtag acgtcgtccc
     7141 tattttcggc ggcggcgtaa tcaacatacc agaattagta tttgcaggcg cattgccatt
     7201 aacggagctg accggtgccg gtggatttga agtgtcagaa cagcctgcca gccatagcga
     7261 aaccagtgac aaagccgcaa tgcggcgaac ggtgaatttt gggcttcccg cgctcattta
     7321 tcccccagga aaaattggtt aataaccagt gacataatta ccgtgcaagg caccctactg
     7381 aacactggaa aagatgttca cgatacgctg acctacggca aaataaccag gaaaaatcca
     7441 ggtatttcct cacgttttaa gccagctcac ccttcactaa agggacaaag cgcacggcct
     7501 ccacggtatc gataataaat tcgcctcccc gacgacgcac ccgtttcaaa tactggtgct
     7561 cctcccctac gggtaagacg agaatcccgc cttcgtccag ctgcgtcatt agcgcagttg
     7621 gaatttccgg cggtgccgcc gtaacaatga tagcgtcaaa cggcgcacgt gcctgccaac
     7681 cttgccatcc atcaccatga cgggttgaaa cattatgtaa atcaagattt ttcaggcggc
     7741 gacgtgcctg ccactgcaag cctttaatcc gttcaaccga gcaaacatgc tggacaagat
     7801 gcgccaggat tgccgtttga tatcccgaac cagtgccaat ttccagcacc cgcgactgcg
     7861 gcgtcagctc gagtaattcg gtcattcgcg ccaccatata cggctgcgaa attgtctgcc
     7921 cctgacctat cggcaaggcg atattgtccc aggctttttg ttcaaacgct tcatcaacga
     7981 atttttcacg cggcacggcg gcaagtgcat tcagcacctg ctcatcctga ataccttgcg
     8041 cacgtaattg atccagaagt gcttgtacgc gtctgcttac cattgcgtgc caactcccac
     8101 gctgtttaac cagtctgaaa ccacatcttg cgcgctatgc gcagttaaat ccacatgcag
     8161 cggcgtgatg gagacatagc cctcatctac cgcagcaaaa tcggtccccg gaccggcatc
     8221 acatttaccg cccggcgggc caatccagta cagcgtattg ccgcgcggat cttgctgcgg
     8281 gatcacctga tctgccggat gtcgtgtacc gcagcgcgtc acgcgaatac ctttgatttg
     8341 atccaagggt aaatccggaa cgttaatatt aagaatacgt ccggtgcgca gcggctcttt
     8401 acacagtgcg cgcaaaattg aacaggttac cgccgcggca gtgtcgtaat gtttatgccc
     8461 gtcaagcgag acggccagcg ccggaaaacc taaatgacgg ccttccatcg cggcggctac
     8521 cgtaccggaa taaataacat catcccccag attcggcccg gcgttaattc cggacacaac
     8581 aatgtccggg cgcggacgca tcagagcatt cacgccaaga tagacacaat cggtcggagt
     8641 tcccatttgc acagcaatat caccattttc aaaggtaaac gtgcgcaggg aggattccag
     8701 tgtcagagaa tttgaagcgc tgctgcggtt acgatcgggg gcgaccacct gaacgtcagc
     8761 aaactcacgc aaggctttcg ccagcgtttg tataccgggt gcatgtaccc cgtcatcatt
     8821 actcagcaat atgcgcataa tcacctgttg tgttgataag ttcccttaca acgctggttg
     8881 caaaactacc cgccggaagc cagaaacgga tctctacggt gacgtcatcc caccagttcc
     8941 agcttaattg ttgcggatac agcagcatcg ctctgcgcgc ggcttcaact ttttcgcgca
     9001 ccagtaaagc ttgtaattca gtttctgcgg cgacagctgc ttgttcgaat gccagcgctt
     9061 cacgctgagt tccccattcg ccactgcccg gcaatgcggc ggttatcatc agctctttat
     9121 cgttgacgcg acgctgtaat tccgccagtt cttcggtggt tgctacaaac cagctaccac
     9181 gtccggctaa ttgtagcgca tcgccgtcaa caacttgatt aacgtctgct tttttgaggc
     9241 gctcagcaac aatctgatta aacaacgcac tgcgggctgc cgacaaccaa aaactccgtt
     9301 tattgcgatc gcgcaccgga gtattggttt gcgcccagcg cagcgcgccc tgcaagttgc
     9361 taccgccaat cccaaaacgt tgggcaccga agtagttcgg tacacctttt acgcaaatat
     9421 cgatcagacg ttgttcaacg tcatcgcgat tgctcacttc gcgcagaacc agggtaaagg
     9481 cgttaccttt cagcgcccct aaacgcagct tgcgcttgtg ccgcgcatac tccagcacct
     9541 ggcagccttc cagttgaaag gcgctcagat cgggcatttc cttgcccggc acgcgagcac
     9601 ataaccactg ttccgttaca gcatgtttgt ctttttgccc agcgaagctg acttcacggg
     9661 aatgaatttt caggaatttc gccagtgcat ccgccacaaa acgggtattg cagccgtttt
     9721 tgaggattct aaccagaata tgctcacctt caccatcagg ctcaaagccc aaatcttcca
     9781 ccaccacaaa gtcttccgga ttggctttca gcagcccggt gccttgcggt ttaccgtgga
     9841 ggtaagtgag attatcaaac tcaatcattt tgttgcctta atgagtagcg ccaccgcttc
     9901 acaggcaatc ccttccccac gtccggtaaa tccaagtttt tccgtagtag tggctttcac
     9961 gttaacatca tccatatggc agccgagatc ttcggcaata aacacgcgca tttgtggaat
    10021 gtgcggcaac atcttcggtg cctgagcgat gatagtgaca tcgacgttgc caagggtata
    10081 acccttcgcc tgaatacgac gccaggcttc gcgtagcagc tcgcggctgt cggcaccttt
    10141 aaatgccgga tcggtatccg ggaacagttt gccgatatcc cccagcgccg ccgcgccaag
    10201 caatgcatcg gtcaacgcat gaagcgccac gtcgccatca gaatgcgcca gcaatccttt
    10261 ttcgtaagga atgcgtacgc caccaatgat aattgggcct tcaccgccaa aggcgtgtac
    10321 gtcaaaaccg tgtccaattc gcattatgta ttctcctgat ggatggttcg ggtgaggtaa
    10381 aactcggcca gtgccaaatc ttccgggcgc gtgactttaa tgttatccgc gcggccttcg
    10441 accaactgcg gatgaaatcc gcaatattcc agcgccgagg cttcgtcggt aatggtagcg
    10501 ccttcattta gagcgcgcgt cagacagtca tgtaacagct cacgagggaa aaattgcggc
    10561 gtcagcgcgt gccataagcc gttgcgatca acggtatgag caatggcatt tttgcccggt
    10621 tcggcacgtt tcatagtatc gcgcactggt gcggcgagga tccctcccgt gcggctggtt
    10681 tcgctcaacg ccaacaatcg cgcgaggtca tcctgatgca aacaaggacg agcggcgtca
    10741 tgcaccaata cccactgcac atcgccagcg gctttcagac ctgccagcac ggaatcggca
    10801 cgctcatcac cgccatctac aacggtgatt tgcggatgat tcgccagagg aagttgtgca
    10861 aaacggctat cgccaggact tatggcaatg acgacacgtg tcacccgggg atgcgccagc
    10921 agcgcatgca ccgagtgttc aagaatggtt tgattaccga ttgagagata ttgcttagga
    10981 cattccgttt gcattcgacg gccaaatccg gccgccggaa ccacggcgca aacatccaaa
    11041 tgagtggttg ccatgttaat tcccgggctg atttatcgat tgttttgccc cgcagactgt
    11101 gcgcgcttcg acgcgtcagg caccagacga taaaaagttt cgcccggcct ggtcatgctg
    11161 agttcattac gcgcacgctc ttcgagcgcc tcctggccgc cattgagatc gtcaatttcg
    11221 gcaaaaagtt gatcgtttcg cgctttaagt ttcgcgtttg tagcttgcag tgccgccaca
    11281 tcatcattga cgcgggtata gtcatgtata ccgttcttac cgaaccacag cgaatactgt
    11341 agccagacca gaatagccag caacagcagc gttagtttac ccatcctgcc ccctgaaaaa
    11401 ctgcatcatc atcccatgca tccgaagacg actctacatc ctctgttggg gataccgcga
    11461 caacgcgggc aaatgtacca catttgtcca ttgttacgta tacccagggc gtgcagaaca
    11521 taatctcatt attagttacg gtttgaatta tgaacagagg agacaagaaa gtacaaatta
    11581 gcccagtagc cacataaaca gtgcgccaaa cataatgcct actgtcacca gggtgaaaac
    11641 aatactgtag cgtagctttc cgtccatcaa tgaatgcagc gcaatcccca ccactaccgc
    11701 gacaggcatc agtgccagaa agaaaggcca ggtgtagata aagaagaaca gcgtgttaga
    11761 gccataaatc aacatcggca tcgccagcgc aaataaccag gagataaaac cgaccacggc
    11821 accaggcagt gaccatgtag tttcttcatc ctcagtaagg ctgtcgttat ttgttagtgt
    11881 aatgttatgg ctattacgca tatttgatcc tgttactttg acgaaccggg catggaaacc
    11941 cggtggtgtc tcaggatctg ataatatcgt tctgtctcaa cagatctaat aattgctgta
    12001 ccaaatttgt tactaattgt tcaccattga gatgaatttc tgccgattca ggctcttcgt
    12061 aaacggaatc tattcccgta aagttgcgca gttcaccggc acgcgctttc ttatataagc
    12121 cttttggatc gcgggcttcg caaatcgcca gcggcgtatc gacaaacact tcaataaagc
    12181 gcccttctcc tacgcgttcg cgaaccatct ggcgttcggc gcggtgtggc gagataaatg
    12241 cggtcagcac caccagtccg gctgcaacca tcaaattcgc cacttcaccg acgcgacgga
    12301 tattctcttt acgatcggca tcgctaaaac cgagatcgct gcataatccg tggcgaacat
    12361 tgtcgccatc cagcagatac gtactgacgc cgagtttatg taacgcctcc tccagcgccc
    12421 cggcgaccgt tgatttaccg gacccggaga ggccggtaaa ccacagcact acaccacgat
    12481 gaccgtggtg tagttcgcgt tgttgcacag tgaccggatg actatgccag acgacgtttt
    12541 cgtcatgcag cgccattatt tatcccccag caaatcgcgc gcgccccagt gcggaaagtg
    12601 gcgacgaacc agggcattca attccagttc gaatgcactg aattcagatg acgcagctgt
    12661 tgcctggcta actggctcgt gcaccatacc ggcacccacg gtcacattgc tcaggcgatc
    12721 gataaaaatc agcccaccgg taaccggatt ttgctgatag cgatctaaca ccagtggctc
    12781 gtcaaaagtg agatccacca ggccgatgcc gttcagcggc aggttttcaa cttcgcgttg
    12841 ggtaaggtta ttaatatcaa cctgataacg aatgccatca acacgagcac gcgtcttctt
    12901 accggcaatt ttgatgtcat aactctggcc cggggaaagc ggctgttccg ccatccatac
    12961 cacatccacc gacgcactct gcaccgccgg taacgcttcg tctgccgcca gcagcagatc
    13021 accacggctg atatcgatct catccgtcag caccagggtg atcgcttctc cggcaaagac
    13081 ttcttcgcga tcaccatcaa aagtcacgat ccgcgcgacg tttgattcca caccagaggg
    13141 cagcactttt acacgttgcc cgacttccac gcgaccggat gccagcattc cggcgtagcc
    13201 acgaaaatcg agatttgggc ggttaacgta ctgcaccggg aagcgcattg gctgggcatc
    13261 caccactcgc tggatctcca cggtttccag cacttcgagc agtgtcagac cgctgtacca
    13321 cggcatactt tcactttgcg aagccacgtt gtcaccttcc agtgcggaga gcggcacaaa
    13381 gcggatatcc agattacccg gcagctgccc ggcaaaggtc agataatctt cacgaatacg
    13441 ggtgaacgtc tcttcactgt aatccaccag atccattttg ttgatcgcca cgaccagatg
    13501 tttgatcccc aacagtgtgg agataaaact gtgacgacgg gtttgatcga gcacgccttt
    13561 acgggcatca atcagtaaga tcgccagttc acatgtcgat gcgccagtcg ccatattgcg
    13621 agtgtactgc tcgtgccctg gggtgtcggc gataataaat ttacgcttct cggtagagaa
    13681 atagcggtag gccacatcaa tagtgatgcc ctgttcgcgc tcagcttgca ggccgtccac
    13741 cagcagagcc agatccagct tttcgccctg ggtgccgtga cgcttactgt cgttatgcag
    13801 cgatgagagc tgatcttcgt agatttggcg ggtatcgtgc agcagacgac caatcagggt
    13861 acttttgccg tcatcgacgc taccacaggt cagaaaacgc agcaggcttt tatgttgttg
    13921 cgcaatcatc caggcttcga cgccgccttc attggcgatt tgttgtgcaa gtacggtgtt
    13981 catcttaaaa atacccctga cgttttttca gctccatgga tcccgcctgg tcgcggtcaa
    14041 tcacgcgacc ctgacgttca ctggtggtgg aaaccagcat ctcttcgatg atttccggca
    14101 gtgtttgtgc atttgactcc accgcaccgg tcagcggcca gcagcccagc gtacggaaac
    14161 gcaccatccg ttttttgatc acttcgcccg gttgcaggtc gatacggttg tcatcaatca
    14221 tcatcaacat accgtcgcgt tccagaaccg gacgttccgc agcgagatac agcggcacaa
    14281 tgtcgatatt ttccagccag atgtattgcc agatatcctg ctcggtccag ttagagagcg
    14341 ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt gtagttgtgc cacagctccg
    14401 ggcgctgatt tttcggatcc cagcgatgga agcggtcacg gaaagagtag attcgctctt
    14461 tagcgcggga tttctcttcg tcacggcgcg caccaccgaa ggcggcatca aaaccgtatt
    14521 tgttcagcgc ctgtttcagg ccttcagttt tcataatatc ggtatgtttc gcgctgccgt
    14581 gcacgaatgg attaatcccc atcgccacgc cttccgggtt tttatgcacc agcagctcgc
    14641 agccgtaggc tttcgccgta cgatcgcgga actcatacat ctcgcggaat ttccagccgg
    14701 tatcgacatg cagcaacggg aaaggcagcg tacctggata aaacgccttg cgcgccagat
    14761 gcagcatgac gctggaatct ttaccgatag agtagagcat caccggattt gagaattctg
    14821 ccgccacctc gcgaataatg tggatgcttt ccgcctccag ttgccgcagg tgagtaagtc
    14881 gtttttgatc cataaccgtt cctttgcaat accgctattt tcttgccatc agatgtttcg
    14941 actataggga gcgtaagaga acgaatgaaa ttaccaatta gaatgagtag ttccttaacg
    15001 gaataacgat ttggcaaagc taatatcaaa aagtgcttaa ggcaccggat ttcgggcgtt
    15061 taggaagatt tgaaattgtt ttagcgcagc ggcagtttca tactatggcg gtaaaaaaat
    15121 ttgcatggta tttaaggact cactatgttt tccgcattgc gccaccgtac cgctgccctg
    15181 gcgctcggcg tatgctttat tctccccgta cacgcctcgt cacctaaacc tggcgatttt
    15241 gctaataccc aggcacgaca tattgctact ttctttccgg gacgcatgac cggaactcca
    15301 gcagaaatgt tatctgccga ttatattcgc caacagtttc agcaaatggg ttatcgcagt
    15361 gatattcgga catttaatag tcggtatatt tataccgccc gcgataatcg caagaactgg
    15421 cataacgtga caggaagtac ggtgattgcc gctcatgaag gcaaagcgcc gcagcagatc
    15481 atcattatgg cgcatctgga tacttacgcc ccgctgagcg atgctgacgc agatgccaat
    15541 ctcggcggac tgacgttaca aggaatagat gataacgccg caggtttagg tgtcatgctg
    15601 gaactggcag aacgcctgaa aaatacgcct accgagtatg gtattcgatt tgtggcgacc
    15661 agcggcgaag aggaaggaaa attaggcgct gagaatttac tcaagcggat gagtgacacc
    15721 gaaaagaaaa atacgctgct ggtgattaat ctcgataact taattgttgg cgataaattg
    15781 tatttcaaca gcggtgtaaa aacccctgaa gcagtaagga aattaacgcg cgacagggcg
    15841 ctggcaattg cacgcagtca cggaatagcc gcaacgacca atccgggttt gaataaaaat
    15901 tatccgaaag gcactgggtg ttgtaatgac gcagaaatat tcgacaaagc gggcattgct
    15961 gtactttcgg tggaagcgac taactggaat cttggaaata aggatggtta tcagcaacgc
    16021 gcaaaaacac ctgccttccc ggcgggaaat agctggcatg acgtaagact ggataatcaa
    16081 caacatattg ataaggcact tccaggaaga atagaacgtc gctgccgtga cgttatgcgg
    16141 ataatgctac cgttggtgaa ggagctggcg aaggcgtctt gatgggtttg aaaatgggag
    16201 ctgggagttc taccgcaggg gcggggaatt ctaagtgata cccatcatcg catccagtgc
    16261 gtccggttta cttccgggaa aaccgcaccg tattgcgccg gtttatctcc gctggagcgg
    16321 ggaacacctc tggcgaatag aaagcaagcc aggttttgac cagtcgatcg tacatggcat
    16381 tggccgttgc cattgttggc aggccaaaaa acagcccctg agcgttaccc gcagccatca
    16441 acctgtgcgc caggataagc gccgcttctg ttttacctgc gccggtcaca tcttccagaa
    16501 taaacaactg tggtcctggc tgattgatat ccagatccag caccttttgc tgtaacggtg
    16561 tcgtattctc aataaaagga aacagcgtat taattccggt gaaaggcgcg gtttctgctt
    16621 gtggcggaaa gacggttaat gcgttttgag cctgaactaa agccttttgc cagtaatctt
    16681 caatatccat tgcctgtgcg acgcgtggaa aaaatcgcgt tgacgaccct gtccagtctg
    16741 caagtacgac ggttgcagag atataccagg aaagttgttt tataagttca acgccctcgt
    16801 catcatccca gaatgtgggg atctctattc gcggaaacag tggcttgatt tcaaggagaa
    16861 aatctcgtgc tgcagctttg tcttcaggca gaaaattatc caattcatcc atacgatcag
    16921 ggggccgacc atgatgccct gtcgtcacgg gcatccacat ttctatcact cgcctaagtt
    16981 tgcgcggaga gagcgaagat gaaggaagta actcctgaca ttcacttaaa taatgattcc
    17041 acaaccagta gcccagcgtt gaatgagaga ttttttcgta attttttctg gcaccttcct
    17101 gaatcttgaa ttcagggggc aggtaaagtt gttgaaaaga gcgggcaaat tttccaatat
    17161 cgtgccagca cagcaaccag gcgaaaaatt gagctgcctg ttccttatca gaaatcccta
    17221 attgacgaaa ataatcagcc agcccgaagc aatttctttt aaccattaaa tagcccattg
    17281 cggccacatc cagcaaatgc cagcaaagaa ggtgatagtc gtcgctaccc tctttctcgc
    17341 cacgtcggtt ttttccccag aaatcaaaga aagtcacaat atttttatcc ttcagtaaac
    17401 ttaaaggata tttacgcata ttttaaaaac tatctgtgat atatatcagc caataaaaca
    17461 tttacattat gaataaatta atgattttca tttgaaattc ataataataa atagagacta
    17521 tatattcaca taaaaaatat aaattttaaa aaagggtgca ggagaccaaa ccatcacaat
    17581 tcagtacgcg gggttttacc cccgcgtggt ggctacttct tcgattcgta agcgagaact
    17641 gccttaaact ccataccacg ttctctgata ctcaactcac ataacgagtc acctaccatc
    17701 aacgtgaatc ccagtactgt acaacacagt acgatgattg ccacaagggc atattttgtc
    17761 agcatgtctt gcctccctgc aaagaagaga ctaacatccg aattgctacg ttcgaacgcg
    17821 agcctcaggt tgatagaaat atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc
    17881 ttaacgccag acagcctcaa gcacccaacg ccattctata cctgttaatt ttccctgcga
    17941 tgttcacctt taaatgccgg aatgctgctc gcaaaataaa aacgccccca tcatttctga
    18001 cagaggcgtt taatttatcc ggcaatattt acccttcgtg taatccacat tcccttttta
    18061 agccaaagaa acgcgtttct tcttccgcca tgccgggttc ccatttacgg gttgtatggg
    18121 tatcgcccac cgataaatat ccttcgtccc ataatgggtg atatttcagg ccatgttttt
    18181 gcaggtactg ataaatagtt cggttatccc agtcgataat cggcagcact ttaaatacgc
    18241 cacgctgaat tgccagcacc ggtaaattgg cacggctgcc ggattgttca cgccgcaggc
    18301 cagcaaacca cgtttgcgca ttcagttctt tcagcgcccg gttcatcggt tcgactttgt
    18361 tgatgtcatt gtacttttca atgccttcaa cgccctgttc ccacagtttg ccgtagcgtg
    18421 cttcctgcca ggcggcgctt tcggtagcgc ggtacacttt caggttgagc ttgagtttgt
    18481 ccgttaactc gtcaataaag cggtaggttt ccgggaacag atagccggtg tcggtgagga
    18541 tcaccggaat atccgggtga atctgattca ccagatgcag gctcaccgcc gcctgaatgc
    18601 caaagctgga agaaagtaca tattcaccgg gcagattatc cagcgcccag gcaacgcggc
    18661 cttcagcgtc cagtttttcc agttcggcgt tagtttctgc cagcgccaga atgcgatcta
    18721 ccttcggcag ttcgttcagg gcgtttagat cgagtttgga catagattcc tcactgtttg
    18781 ccttgcctga tgcgacgctc acgcgtctta tcaggcctac aagaccgggc tgatggttaa
    18841 tcccacaaat cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag
    18901 tcgccgaagc cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc
    18961 gacgccagga tttccggctc ggtgatgttt tctttataca tccgtgggat acgtgtccca
    19021 atgcggttgc cgccaagatg caggttgtag cgacccggtg ctttacccac caggcccact
    19081 tccgccagca tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc
    19141 tcatcgctga caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg
    19201 aaacgctccg cttccgccat cgccagcggg caagtcggga atgacacgca ggccatcgag
    19261 ttttcacgct gcggcgtgac ggcattcatt aacccgctct ctttggcaat tttctcgatc
    19321 ttcgctttct cgctttccgg cacaccggcg atgatcagat tctggttcgc cgtaatgcgg
    19381 aaatcacctt tgtggatctt cgcgatctcc agcaggccag ttttcagcgg acgccccgga
    19441 taatcaagga tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgccc
    19501 ttaacccagc caatacgatc gccgcgtcct gtaaattcat acgggcggat cggttcaaat
    19561 ttgatccccg cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagt
    19621 gtgtattttg ttttggcatt tttacgatcg gttcggttac cccagtcacg ctgagttgtc
    19681 acgacggctt ccgccaccgc tagcgtatgc tctagcggca gatagccaaa ctcactcgcc
    19741 gtgcgggcgt aggttttctt gttaccgtgt tcgatagaaa gcccaccgcc caccagcagg
    19801 ttgaagccca ccagcttgcc gttttccgcg atcgccacga agttcatgtc gttggcgtgc
    19861 agatcaatat cgttctgcgg cgggatcact accgtggttt tgaacttacg cggcaggtag
    19921 gtctgaccaa ggatcggttc ttcatcggtg gtggcgactt tttcctgatc gagccagatc
    19981 tccgcatacg cgcgggtacg cggcagcaga tgttcagaaa tcttcttcgc ccactcgtac
    20041 gcttctgcgt gcagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg
    20101 tcgttagcag tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg
    20161 acgttctttt tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg
    20221 ccatagatgg tgttttcacc ggcaaattta tcgatcgcct gccactgttt ggtggtgata
    20281 actccacccg gcagacggca gcgcaacagc atcgcgtggc gcggctccag cttctgttca
    20341 gcacgttcgg cgcggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc
    20401 aggaagttat cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg
    20461 ccgcgcaggt agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc
    20521 actaaaggcc ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta
    20581 cgcgcagctc acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa
    20641 tcacttccag aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat
    20701 aaatgtgggc accgtcattg atccagcgcc acagctccgc gccctgttcg cgcagtttat
    20761 cttgtacgta aactttttct ttttggtcgc gcgaccaggc gagatcgatc cgcgtcagca
    20821 caccctcttt gacgtagcgc tgccactcca cctgatacag gaagtcttcc gtaaaatgcg
    20881 gattgccaaa gaacagccag tttttacctg gcgcttcgtc agcggcgcgt tgctgcataa
    20941 aggcgcggaa cggcgcaata ccggtgcctg ggccaatcat aatcaccggg gtttccaggt
    21001 tagcgggcag gcggaagtta tcgttatgtt cgataaatac gcggacttcg ccctcttctt
    21061 ccacgcggtc agcgaggaag ctggaggcac caccggcacg ggcgcggcct tccacgtcgt
    21121 aacgcaccac accaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcaa
    21181 tggaatagag acgcggggtc agcgggcgca gcagattaat tagcgcttcg gcatcaagct
    21241 gtgccgggga gaaacgcacc atgtcaacaa tcagcgtcgt cgcggcgtaa tgctgtaact
    21301 tcgctttatc gcccaccagc ggcagcagtg tttcactgcg ggtaagcgtg gcgtaattct
    21361 caacaatgtt ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag
    21421 gcaacgtttt gccctcgaca gtgacaggtt catcgcctgt cagccacagc agttcgacaa
    21481 gttctttcac cagtgccgga tcgttctgat accagacgcc cagcgcgtca cccggctggt
    21541 aacgcaggcc cgagtcgcct aagtcaattt cgatatggcg aacgtctttt tcagagttac
    21601 gcccagtaat tttctggtta acagagaggc tagccaccag cggcgcgtct ttgctgtacg
    21661 ggctggtgtg gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag
    21721 gcgcacgcga tttaagcgca tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt
    21781 attcaacatc agaatcgaca cggtcgagca ggcgttcacc acccagttcc gccagcttgc
    21841 tgtcaaaatc tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca
    21901 cggcaaacgc ggtgttttcc agctttggcg cttttttgga gaacaggaac ttatgcagcg
    21961 cgacggcttc ttccggcggt tccccttccc cttgcgttga cgtcactacg atgagcagtt
    22021 tttcgctggc gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt
    22081 ttgctgctaa taaatcatcg cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg
    22141 aagcagagat aatcgttata cccggcattt ctgcggcggg cgctggcgtc actgcaagcg
    22201 caacaggctg ctggttgagc acgccccaga aatagccaga aacccaggca agctgggtag
    22261 gcgttaaatc ggtcgtggcc gcctgaaggc gtgccagttg ctccgggttc aacggaagca
    22321 acgcggaagg tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt
    22381 ctgcgccctg tcagcgccca taaaacagaa gagaaggtaa ggttaacggg gtgaacggtg
    22441 tggattaaag acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata
    22501 gtctttaaca acaaaataga ttaaccaacc taatgaaaaa caaatgaatt tagccaatca
    22561 ttaagataaa tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc
    22621 tttccggtac tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt
    22681 atttaaagat ttcaccttcg aagccgctca ccgcttacca catgtcccgg aagggcataa
    22741 atgtggtcgc ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga
    22801 tccgcatacg ggctggatta tcgatttcgc tgaactgaaa gcggcgttta agccaactta
    22861 tgagcgcctc gatcaccatt atctcaatga tattccaggt ctggaaaacc caaccagcga
    22921 agttttagca aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt
    22981 gatggtaaaa gaaacctgca ccgcagggtg tatctatcgc ggcgaatgat aagagtgtgt
    23041 cggcggtcaa tttcccttta gtaacgctat gttagggtgt tgtgttctgg atatctgggg
    23101 catgacatgg aagacgactg cgacattatt attattggtg ccggtattgc aggcaccgct
    23161 tgcgcgttac gctgcgcgcg agcggattta tccgttttgt tactcgaacg cgctgaaatc
    23221 cccggcagca aaaatctttc cggcaggcgg ttatataccc atgcactcgc ggaactcctc
    23281 ccgcaatttc atctgaccgc gcctcttgaa cgacgcatca ctcacgaaag cctttccctg
    23341 ttaacgccgg atggcgcaac gacgttttcc agcttacagc ccggcggtga atcctggagt
    23401 gtattacgtg cacggttcga tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa
    23461 tgcatccccg gagcgacggt ggatgcactg tatgaagaaa acggcagagt ctgtggcgtt
    23521 atttgtggtg acgatattct ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc
    23581 gtcctggctg aacgtcacgg gttagtgact cgtcctgctg gcgaagcgat ggcattgggg
    23641 atcaaagaag tgctgtcgct ggaaacatcc gctattgaag aacgttttca tctggagaat
    23701 aacgaaggcg cagcgttgct gttcagcggc ggaatctgtg atgacttacc cggcggcgca
    23761 tttctttata ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt
    23821 acgcaaagtc gtgttccggc aagcgagctg ctgactcgct ttaaagcgca tccggcagtg
    23881 cgcacgctta tcaaaaacac ggaatcactg gagtatggtg cgcatctggt gccagaaggt
    23941 ggcttgcaca gtatgccggt gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg
    24001 ttgcgcagtt gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctgactggc
    24061 gcacaggcgg cggcacaaac gctaataagc gcctgccagc accgcgagcc gcaaaatctg
    24121 tttgcgcttt atcatcacaa cgtagagcgc agcctgctat gggatgttct acaacgttat
    24181 cagcatgttc cggcgctttt gcaacgccct agctggtatc gggcgtggcc tgcgttaatg
    24241 caggatattt cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag
    24301 ttattctggc gtcatttacg tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg
    24361 agtctgcgat gtctgtagcc cgtaatctct ggcgcgttgc tgatgcgccg cacattgttc
    24421 cggctgactc cgttgagcgt cagatggtac aacggttgat taacgcctgt ccggtaggtc
    24481 ttttttctct cacacaggaa ggtggcttac gtattgacta tcgcggctgc ctggagtgtg
    24541 gcacctgccg tttgctgtgc gacgaatcca cactacaaca gtggcgctat ccgccttccg
    24601 gattcggcat cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac
    24661 ctgctccgcc agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggcc
    24721 atcgattctg aatgccagtt tatttccgtg ttgtacggca atatctgcac catcagtaat
    24781 atcgttaaaa agattaagaa cgccgggaaa tatgctttta ttcatgttga tttgctggaa
    24841 ggcgcgtcaa ataaagaagt ggtgattcag tttttgaaac tggtgaccga ggcggatgga
    24901 attatcagca ccaaagcatc gatgctaaaa gcggcaagag cggaagtttt tttctgtatt
    24961 catcgcctgt ttattgttga ttcgatttcg tttcacaaca ttgataagca agttgcgcaa
    25021 tcgaatccgg attgtattga gatcctgcca ggctgcatgc ccaaagtgct gggctgggtg
    25081 acggaaaaaa tccgccaacc gctgattgct ggcgggctgg tatgcgatga agaagatgcg
    25141 cgtaatgcga ttaacgcggg tgtcgtggcg ctttccacca cgaataccgg agtctggacg
    25201 ttagcgaaaa aattgctttg acgggataat cgtaaccaat tgaatttgtt ttgatttgtt
    25261 ggccgcacgc cacatccgac attcagcgcc tgatgcgacg cttaacgcgt cttatcaggc
    25321 ctacaggtcc ccagtccaga accgtaggca ggatcatccg gcagcacgtt attggtattc
    25381 tgcgtgaatg tttgtcacca gtgcctccag caccactttc cagtcatcaa ccacgtcaat
    25441 atctgcctgt gaaaataccg ctgcgctggc gtcatgatta atcgccacca taaacttgct
    25501 attgcgtacg cccgccatta acgctgctgc gccggaagcg ccaacaacaa tgcacacctc
    25561 aggtgccagc agatgtccgg aaataccaat cactttttca gcatcaacgc caccgttcat
    25621 cactcgcgcc cgactgtagc ccacttcggt tcccagcttt tctgccagca taacgatttc
    25681 ctggttatct gcctctccgc cctgccccac caccagcacc cgtctggcct cagctaatgg
    25741 gtcgcggcta acatttttca gatcctctgt gctaaccagc cagtcgggaa gcgcacctgg
    25801 gacaatgttc agttgctgca ttccagaggg caacgtggcg tttttcgctg ctcccgcctg
    25861 acgcgccagg gaaaggcata acgggcgttt ttcagtttgc agcgtcgccg ttaacgcatt
    25921 tccccagtgg gatttgcgca tgcttacggt tggaatatct aacgaagtca cctgacagat
    25981 gcttgcacca tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa acgttcccgg
    26041 cggaaagaga acaacgtccg caggcgttcg ctgccactgt tccaccatcg catccagcac
    26101 ctgttccgcc actacgggct gaggttctat ttgccagtgc gccagcgtac aaccgctgaa
    26161 atcctgcgcc gccagccagc tcgctattgc ggcattctcc tgatttattg tcacaattgc
    26221 gatattcatg gctgcatcct ttggcgtaaa tagtcctgcc atagcttttg agctttttct
    26281 gctaccgttt gcccatcaat cagcgtcgct ccgcatcgtt gctctgctct tgccagttgc
    26341 agacactgca tcgctggcat ttcggcagca acagttttac ggataatttc tgctttcccc
    26401 gcagccattc gctggcgcat tcccggtact ggcaaggcca cttcaccgca ctgacgcacg
    26461 gcaatcaccg caggcaagcg aacccggcaa cagcgcaacc catgttcagt acgctgttcg
    26521 agggtgataa acagcgcgtc gagagtgaaa cgctccacct gggtgaagca gggccagccc
    26581 agcatttccg ccagcaaaaa tggcgtttgc ccattctgcc cttcgctgct ttggcagccg
    26641 gtaattatca gatccagcgg attctgacgc tgccattcgg caatatggcg agcgacaaat
    26701 tccggcgcaa agcgcagatc tgccgccgtc tccagcaata cagcttcctc aaacccaaga
    26761 gccatgagat agcgcagcca gtgcaacgcc cgttcatccc ccatgctcaa cgcggttaaa
    26821 gacatcggcg taccgttttt tctctgcgct aacagcagcg cggcagcggc ctgttcatca
    26881 gcaccgagta aacttcgcag tagcgaaaca tccggtccgc tattaccctg agccgccgcc
    26941 tgccactctt tttccgccag cattccggca tccggttcgg ctttaaacgc taacagaatg
    27001 ttcattcgca cctcctacat ttcccgccgc caccagcggg agggctttag tttctggtgc
    27061 ccatagccag gtcacaacaa aaccaaccaa caacactgtc gccaggagca ataaggtgac
    27121 ttgcattccc cactgcgcca gcacccacgg cagcaggcca gtacttaccg ccgcgccaag
    27181 tcgactcatg gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag gaaaactttc
    27241 cgcaggcaaa atgcccacca gattactgac tgccgaaatg gtggtgctga agagaacaaa
    27301 aagcagcagc gttaatgaac tgccggaagg caaacaggcc attactacca gcgttgccgc
    27361 cagcagcaaa aaacttccca gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa
    27421 tcccagcagc gcgcccacaa ttaacaacgc attaagcatc aggctggcag tcagcgcatc
    27481 ttccagacca atagtctggg cgatagttgg cagccaggta taaatcacaa accatgggat
    27541 tacgaggcag acaaagaaga cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa
    27601 caaggttttg atgtgtttat gggtcgccgt taccacttca tcgcccagta aaacatgggg
    27661 accaaaatag cgatgcacga tagcgtgagc ttctgcaaaa cgcccctggc acagtagcca
    27721 acgtggcgat tctggcgttc cccagcgtaa taacgtaatc aacaacgcgg gcaaagctgc
    27781 cgatgccagc aaccagcgcc aggcctccgg gttttcggaa ataaagtgat gtccggcaat
    27841 acttgccaac acatagccta cggtccacac cacgctgaat gcgcccagca aaataccgcg
    27901 atggcggcgc ggggaaaatt cagccagcaa ggtgtgacct actgagtaat cgcctcccag
    27961 accaatgccg atcaaaatgc gcagcccaat aagatgctct ggcgtggtgg caaaaaattg
    28021 caagaacgaa gcgagcgtaa tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat
    28081 atggtcggag atccacccaa gaaccaggct gccaagaaac aaaccaagga gcgccgagcc
    28141 gccgatcatt cccgccataa acggcgtcag ttgcatggcg ggcgtaagtt gaataatggc
    28201 gtaaccaatg acgccgagaa cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc
    28261 aattcgacag tgaaaacggt taagcggtaa atcatccatt cgcaccggtg aagtgttcat
    28321 ctttacccct tcattgtccg gtaataagaa cggcttctcg cctgagaagc cgaaaaggca
    28381 acgccccgtc atcgcggaac gctgcctgat acattatttt tcaatcggat agatagtacc
    28441 cgtattcata atgccgttag gatcgaactg ctttttcagc ccttccagca acgcccacgc
    28501 gctgccgtgt tccagcttgc tccagtgaac gcgatgttta ccaatgccat ggtggtgcac
    28561 catcgaaccg ccgaggcgaa tggtttcttc gcagatgatt ttgttgagcg gattgtagta
    28621 cttgtcgatt tcctcttccg gcttacagtc aacaacgttg tagtcataga cgaagtacat
    28681 gttggtgccg ttctgatagc tatgagagga gtgaccgccc agcatggtga tgtcgtcggc
    28741 gtgcgggaac tcagtacgaa tacggttaat aacgctttcg tagatttcgt ggatgcagct
    28801 ccagcagccg gacacttcgg tggtaaagcc catgttgccg gttttgagga tctgcacacg
    28861 ttcggcagcc actttatccg gtccccagtt caggttgtta aaccaggttt caatcagttt
    28921 gctgtccacg cgctggcact gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt
    28981 cgccttcgca atgcgagggt taccttcagc cataaagatc agcacccatt ttccgtcggc
    29041 aaaatgggtg aagtgttggg tgccatcttc agcgtcatac aaacgagcga tcgacggacg
    29101 atacccttcc accatgattt cacgcaggat gttgaagccg gttttcatgt cttccaggat
    29161 atagccgtag aagaggttgt tttccggggt gtatttaaag attttcactg ttacttcagt
    29221 gatatagcac aatgcacctt cgttgccgat gatgatgtga cgaatgtccg ggccagccgc
    29281 gcggcgtggc acgtttttaa tgcgtgtgac ggtgccatct gccaacactg cctccagacc
    29341 aacgaccata tcttcgattg cgccgtagag tgtggagaac tgcccgatac tgcgggttgc
    29401 taccaggccg cccatctgcg ccagcggctt tgactgcgga gaatgccccg tggtgtaacc
    29461 tttttcacgc aacgcgtttt ccagcacttc cagcggaaca ccacattgca ccgtcgcctg
    29521 catattctca atatcaatat taatgatttg attcatggcg gagccgtcga gcaccaccga
    29581 gttttctaca acagtttcca gcccaccttc ggtggcggaa gcaccggtac gcggcacacc
    29641 gttaattttg tgcgcattca taaaattcag cacacgggat acttactctg tggaaccgag
    29701 ttttacgacc gctgccggaa tcggcaaagt ataaatgcca tgaatatccg gaaatttacg
    29761 aaaacggtca atactgtttt tctttaatac tgtttcatcg gtaattacgc gatctgcacc
    29821 aacaatttcc tttagctggt cgacaatcgc tgcgcgagat aaagacatag taattccttc
    29881 ctgataataa aaatggaata atcattaata agagaaaaca gaaaccaccg ttatggatta
    29941 gcgcactaaa taaccgccat cgaccactaa taaatgcccg ttgacataat tcgatgcctg
    30001 acttgcgaga aacacggctg cgcccattaa atcctgagta tcgccccaac ggtttgccgg
    30061 aatatgatca agaacgcgct gattggtttc tggattactg cgtgtcgcca gcataatatg
    30121 ctgttgcata atagccaggg gcgataccat ttacctgaat attatattga cctagttcat
    30181 cacaataagc tttggtgaac ccggcaagag catgtttagt ggcagaatat gcaggtgacc
    30241 attgtccacc taagtaagag aacaatgaac agatattaat aattttgccg cttttctgcg
    30301 ggatcataat ttttgccgct tcatagctta actcgaatgc ggcggtcagg ttcacatcaa
    30361 tcatcggatc ccagtcggca cgaccgaagt ccagcacctt attcagctta caaataccgg
    30421 cattgttaac cagaatatcg acagtaccga agcgctcaca gcaggcagcg ataatcttct
    30481 gcggtgcgcc ttctgcggta atatccacct gcatgaagtc cacttcaaca ccctgttttt
    30541 caatcatttc ctttgtttcg ccgttatctt tgacgaagct aggaataaaa atatttgcgc
    30601 cagctttggc caacgccatt gcaaatgcct gacctaaacc gctattcccg ccggtaacaa
    30661 ttgctgtttt acctttcagg gagaaaaaat ccattgagaa cgcattgaga gattcgattg
    30721 acatagttag ctccaatttc cgtaaggcaa aaaaaagaaa ggtaacttcc cccgcgagag
    30781 ggaagttacc tttctcatta tctctggtaa ctggagataa tttttagcat aacggcataa
    30841 ctgctaatgt gatcgtaatc acagtgtgat attcgtattg tcaaatagtt ttaaaaagca
    30901 gaagcgtgat tactctcaca caacacctct gctttccatg ttatttctta ttgcaattac
    30961 tagagacctg agaaaagtaa tccacctgaa agggcggctt acttttctct tttttttgtc
    31021 tattacaaat gggaatacga tatgcaacac aactcatatc gccgttggat aaccctcgcg
    31081 ataattagtt ttagcggcgg cgttagtttt gacctggctt atttacgtta tatttatcaa
    31141 attcccatgg cgaaatttat gggattcagc aataccgaga taggtttaat aatgagtacc
    31201 tttggtattg cggctattat tctttatgcc cccagcggcg ttattgccga taaattttca
    31261 caccgcaaaa tgattacttc cgcgatgatc attaccggat tactgggtct gttaatggca
    31321 acgtatccac cgctgtgggt aatgctctgt attcaggtcg cctttgcgat aacgacgatt
    31381 ttaatgctgt ggtcggtgtc gattaaagcc gcctcgttgc ttggcgatca tagcgagcaa
    31441 gggaaaatta tgggctggat ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg
    31501 tttaccatgt gggtcttttc tcgctttgca ccggatgaca gcgccagcct gaaaacagtc
    31561 attatcatct acagtgtggt ttacatcttg ttggggattc tgtgctggtt ttttgttagc
    31621 gataacaaca acctgcgcag tgccaataac gaagaaaaac agtcattcca gcttagcgac
    31681 atccttgccg ttttgcgtat cagcaccacc tggtattgca gcatggtgat ttttggtgtc
    31741 ttcacaatct acgccattct gagttactcc accaactatc tgaccgaaat gtacggcatg
    31801 tcgctggtgg cagcgagcta catggggatt gtgatcaaca aaatcttccg cgcgctgtgc
    31861 ggcccacttg gaggcattat caccacctac agtaaagtga aatcccctac ccgcgtgatt
    31921 caaatccttt ccatcatcgg tttgctggcg ttgacagccc tgctcgtcac gaactctaac
    31981 ccgcaatcgg tcgcgatggg gattggcctg attttactgc tgggattcac ctgttacgcc
    32041 tcacacgggc tgtactgggc ctgccccggc gaagcgagaa caccgtctta cattatgggc
    32101 accacggtag gtatttgttc ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt
    32161 atcggccact ggcaagacac cctgcccgct gcagaagcct accgcaatat gtggctgatg
    32221 ggcatggcgg cgcttgccat ggtgatagtc tttacctttt tgctgttcca aaaaattcgt
    32281 actgctgata gcgcccccgc aatggctagc agcaagtaag ccaggaaaat tacaggagaa
    32341 aaatcatgtc gaaaaaatac atcataggga ttgatggcgg aagtcagagc acaaaagtgg
    32401 tgatatacga tctggaaggt aatgtggttt gcgaagggaa aggcttattg cagccgatgc
    32461 acacgccaga tgccgatact gcagaacatc ctgacgacga tttatgggca tcattatgtt
    32521 ttgccggtca cgatttgatg agtcagtttg ccgggaataa agaagatatt gtcggtattg
    32581 gtctgggatc catccgttgc tgccgtgcgt tattgaaagc cgatggcacg cccgctgcgc
    32641 cgttgattag ctggcaggat gcacgcgtaa cgcgtcccta cgaacacact aatcctgacg
    32701 tggcatatgt cacctctttt tcgggttatc tgacgcatcg cttaaccggc gagtttaaag
    32761 acaatatcgc caactatttt ggtcagtggc cggtggatta taagagctgg gcatggagcg
    32821 aagatgctgc ggtaatggag aagtttaata tcccccgtca tatactgttt gatgtgcaaa
    32881 tgccaggcac tgttcttggg catatcacac cacaaggcgc gctggcgaca catttcccag
    32941 ctgggctgcc ggttgtttgt accaccagtg ataaaccggt agaagctctg ggggccggat
    33001 tactggatga tgaaacggcg gtgatttcac tcggtactta tatcgcgctg atgatgaacg
    33061 gcaaagcctt gccgaaagat ccggtggcgt actggccgat tatgtcttct attccgcaaa
    33121 cattgctgta tgaaggttac ggtattcgca aaggtatgtg gacggtgagc tggctgcgcg
    33181 acatgttagg cgagtcgtta attcaggatg ccagggcgca ggatctttca ccggaagatt
    33241 tactcaacaa gaaagcctct agtgtgccac ctggctgtaa tgggctgatg acggtgctgg
    33301 attggctgac caatccgtgg gaaccgtaca aacgcgggat tatgatcggc tttgattcca
    33361 gcatggatta cgcatggata tatcgttcga tactggaaag cgtggcgctg acgctgaaga
    33421 acaattacga caatatgtgt aatgaaatga atcactttgc gaagcatgtg atcattactg
    33481 gcggcggttc gaacagcgat ctgtttatgc agatttttgc cgacgtgttc aaccttccgg
    33541 cacgacgtaa cgccattaac ggttgtgcaa gcctgggggc agcgattaat acagcggtag
    33601 gtctggggct atacccggat tacgcaacgg ctgtcgacaa aatggttcgc gtgaaagata
    33661 tctttatgcc tgttgagagc aatgccaaac gctacgacgc gatgaataaa ggtattttca
    33721 aagacctaac caaacatact gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg
    33781 aattggggaa tgcggattcg atccagagct ggtcgaatgc gtaagtgagg aagaccgggc
    33841 gggaaactgc ccggcctgaa tatacctgaa tggttatccc cgctgacgcg gggaactcga
    33901 ataactgacc aacgtctggc tctctaaatc cggtttatcc ccgctggcgc ggggaacact
    33961 ctaaacataa cctattatta attaatgatt ttttaagcca gtcacaatct accaacttta
    34021 caggatcaca caaacaacac atccattatg ttaaagagca cttaatccat tgattaaaaa
    34081 ggtaaatatt taaaataact ctatacaaac taaaatctac caaacttcgc cgcaataatt
    34141 ttcactccag caaaaaatta acgccgcaga atttgtagag aatgataatg attagtccgg
    34201 gatgggcaag atggatgagt tttcaggggc gacaaataat acaaatgagt ggaatttttt
    34261 acaagttcca cccatttata a
//
LOCUS       DADWTH010000037        33552 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.037, whole genome shotgun sequence.
ACCESSION   DADWTH010000037 DADWTH010000000
VERSION     DADWTH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.037"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            535..672
                     /locus_tag="JNY02_001858"
     CDS             535..672
                     /locus_tag="JNY02_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433408.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="JNY02_001859"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="JNY02_001859"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HBB9433409.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /gene="virB"
                     /locus_tag="JNY02_001860"
     CDS             complement(2999..3928)
                     /gene="virB"
                     /locus_tag="JNY02_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HBB9433410.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="JNY02_001861"
     CDS             complement(4145..4381)
                     /locus_tag="JNY02_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB9433411.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="JNY02_001862"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="JNY02_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HBB9433412.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYNAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="JNY02_001863"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="JNY02_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HBB9433413.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRK
                     GYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="JNY02_001864"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="JNY02_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HBB9433414.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSI
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSL
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="JNY02_001865"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="JNY02_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HBB9433415.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="JNY02_001866"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="JNY02_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HBB9433416.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="JNY02_001867"
     CDS             complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="JNY02_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HBB9433417.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="JNY02_001868"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="JNY02_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HBB9433418.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="JNY02_001869"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="JNY02_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBB9433419.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="JNY02_001870"
     CDS             13569..15185
                     /locus_tag="JNY02_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HBB9433420.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVYYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="JNY02_001871"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="JNY02_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HBB9433421.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="JNY02_001872"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="JNY02_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HBB9433422.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17130
                     /gene="mxiG"
                     /locus_tag="JNY02_001873"
     CDS             16024..17130
                     /gene="mxiG"
                     /locus_tag="JNY02_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HBB9433423.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQSNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFFR"
     gene            17148..17399
                     /gene="mxiH"
                     /locus_tag="JNY02_001874"
     CDS             17148..17399
                     /gene="mxiH"
                     /locus_tag="JNY02_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HBB9433424.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17412..17705
                     /gene="mxiI"
                     /locus_tag="JNY02_001875"
     CDS             17412..17705
                     /gene="mxiI"
                     /locus_tag="JNY02_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HBB9433425.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17711..18436
                     /gene="mxiJ"
                     /locus_tag="JNY02_001876"
     CDS             17711..18436
                     /gene="mxiJ"
                     /locus_tag="JNY02_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HBB9433426.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18433..18960
                     /locus_tag="JNY02_001877"
     CDS             18433..18960
                     /locus_tag="JNY02_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBB9433427.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18902..19597
                     /gene="mxiN"
                     /locus_tag="JNY02_001878"
     CDS             18902..19597
                     /gene="mxiN"
                     /locus_tag="JNY02_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HBB9433428.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19590..19997
                     /gene="mxiM"
                     /locus_tag="JNY02_001879"
     CDS             19590..19997
                     /gene="mxiM"
                     /locus_tag="JNY02_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HBB9433429.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19981..20409
                     /gene="mxiM"
                     /locus_tag="JNY02_001880"
     CDS             19981..20409
                     /gene="mxiM"
                     /locus_tag="JNY02_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HBB9433430.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20539..21171
                     /gene="mxiE"
                     /locus_tag="JNY02_001881"
     CDS             20539..21171
                     /gene="mxiE"
                     /locus_tag="JNY02_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HBB9433431.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21158..22858
                     /gene="mxiD"
                     /locus_tag="JNY02_001882"
     CDS             21158..22858
                     /gene="mxiD"
                     /locus_tag="JNY02_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HBB9433432.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22877..23944
                     /gene="mxiC"
                     /locus_tag="JNY02_001883"
     CDS             22877..23944
                     /gene="mxiC"
                     /locus_tag="JNY02_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HBB9433433.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23957..26017
                     /gene="mxiA"
                     /locus_tag="JNY02_001884"
                     /gene_synonym="virH"
     CDS             23957..26017
                     /gene="mxiA"
                     /locus_tag="JNY02_001884"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HBB9433434.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26030..26431
                     /gene="spa15"
                     /locus_tag="JNY02_001885"
                     /gene_synonym="spaK"
     CDS             26030..26431
                     /gene="spa15"
                     /locus_tag="JNY02_001885"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HBB9433435.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26435..27727
                     /gene="spaL"
                     /locus_tag="JNY02_001886"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26435..27727
                     /gene="spaL"
                     /locus_tag="JNY02_001886"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HBB9433436.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27820..28158
                     /gene="spa13"
                     /locus_tag="JNY02_001887"
                     /gene_synonym="spaM"
     CDS             27820..28158
                     /gene="spa13"
                     /locus_tag="JNY02_001887"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HBB9433437.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28145..29023
                     /gene="spa32"
                     /locus_tag="JNY02_001888"
     CDS             28145..29023
                     /gene="spa32"
                     /locus_tag="JNY02_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HBB9433438.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29017..29898
                     /gene="spa33"
                     /locus_tag="JNY02_001889"
                     /gene_synonym="spaO"
     CDS             29017..29898
                     /gene="spa33"
                     /locus_tag="JNY02_001889"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HBB9433439.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29903..30553
                     /gene="spa24"
                     /locus_tag="JNY02_001890"
                     /gene_synonym="spaP"
     CDS             29903..30553
                     /gene="spa24"
                     /locus_tag="JNY02_001890"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HBB9433440.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30568..30828
                     /gene="spaQ"
                     /locus_tag="JNY02_001891"
     CDS             30568..30828
                     /gene="spaQ"
                     /locus_tag="JNY02_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HBB9433441.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30828..31598
                     /gene="spa29"
                     /locus_tag="JNY02_001892"
                     /gene_synonym="spaR"
     CDS             30828..31598
                     /gene="spa29"
                     /locus_tag="JNY02_001892"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HBB9433442.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31605..32633
                     /gene="spa40"
                     /locus_tag="JNY02_001893"
                     /gene_synonym="spaS"
     CDS             31605..32633
                     /gene="spa40"
                     /locus_tag="JNY02_001893"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBB9433443.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32646..32888
                     /locus_tag="JNY02_001894"
     CDS             32646..32888
                     /locus_tag="JNY02_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433444.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10477 a         4880 c         6711 g        11484 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccctgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc tggtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagttact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg tggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca agtcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgcgttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagagggggg gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatatccttt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgatgtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtagtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgtttttttg
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa atactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtaa gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttg tattaatatt ttggttagca gtaagatcag caattgtaag
    10081 agaaaataat ttgttagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgt
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttatta atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttatt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggga gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgtc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ctaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt ttttagataa aaataagtga ggataaaatg agtgttacag
    17161 taccgaatga tgattggaca ttgagttcat tatctgaaac ttttgatgat ggaactcaaa
    17221 cattacaagg tgaactaaca ttggcactag ataaattagc tagaaatcct tcgaatccac
    17281 agttgctggc tgaataccaa agtaaattat ctgaatatac attatatagg aacgcgcaat
    17341 ccaatacagt gaaagtgatt aaggatgttg atgctgcaat tattcaaaac ttcagataat
    17401 agggagcatt catgaattac atttatccag tcaatcaggt tgatattatc aaagccagtg
    17461 attttcaatc tcaagagata tcaagtctgg aagacgtcgt gtcggctaaa tatagtgata
    17521 ttaagatgga tacagatatt caagtatcac aaataatgga gatggtaagc aatccagaat
    17581 cattaaaccc agaatctttg gccaagttac agacgacgct ctcaaattat tcaataggag
    17641 tatcattagc tggcacgtta gcaagaaaaa cagtttcggc tgttgaaact ttattaaagt
    17701 cttaatttat atgattaggt ataaaggttt tattttattc ttgttgctga tgttgattgg
    17761 atgtgagcaa cgtgaagagt taatttctaa tttatctcaa agacaggcaa atgaaataat
    17821 atctgtgcta gaacgccata atattactgc tagaaaagtt gatggaggta aacaggggat
    17881 ctcggtacaa gtcgaaaagg ggacatttgc atcggcagtt gatttgatgc gcatgtacga
    17941 tttgccaaat ccggagagag ttgatatctc acaaatgttt cctacagatt cattagtgtc
    18001 ttctccaaga gctgaaaagg cccgtttata tagtgctatt gagcaacggc tggaacagtc
    18061 tttagtttct attggtggtg ttatttcggc aaaaatacat gttagctatg atcttgaaga
    18121 aaaaaatata tcttcaaaac cgatgcatat atcagtaatc gctatatatg actcaccgaa
    18181 agagtctgaa ctattagtta gtaatattaa gcgatttttg aaaaacacct tttctgatgt
    18241 taagtatgaa aatatatctg tcatattaac tccgaaagaa gaatatgttt atacaaatgt
    18301 acaacctgtt aaggaagtta aatcggaatt tttaacaaat gaagtaatat atttatttct
    18361 cgggatggct gtactagttg tcattctttt ggtatgggca ttcaaaacag ggtggttcaa
    18421 gagaaacaaa atatgataag aatggatgga atttataaaa aatatctttc aataattttt
    18481 gatccagcgt tctatataaa tagaaatcgg ttgaatttgc cttctgaact gttagaaaat
    18541 ggcgtaatca gaagtgagat taataatctc ataattaata aatatgatct aaattgcgat
    18601 attgaacctt taagcggggt aaccgctatg tttgttgcca actggaattt acttccagct
    18661 gttgcttatt ttatcggtag tcaagagtct cggttaatta atcatagtga gatggttatt
    18721 agttattatg ggggcaaaat cagtaagcaa ggggaggccg ctattcgaag tggtttttgg
    18781 catcttattg cgtggaagga aaatatatct gttggtatat atgaaagaat aaacttatta
    18841 tttaatccaa ttgctttgga gggaaattat acgcctgtag aaagaaattt gtcccgatta
    18901 aatgaaggta tgcaatatgc aaaaaggcac tttaccggta tccagacatc atgcctatga
    18961 tggtgttgtt ataaagagaa ttgaaaagga attatgtaaa accataaaag atagagacac
    19021 tgagagtaag aagaaagcaa tttgtgttat aaaggaggct acgaaaaaag ctgaatcact
    19081 gcgtatagat gctgtatgtg atggttatca aattggtata cagactgctt ttgagcatat
    19141 tattgactat atttgtgaat ggaagttaaa gcaaaatgaa aacagaagaa atattgagga
    19201 ctatataact agtttgcttt cagaaaattt acatgatgag aggatcatta gcactttatt
    19261 ggagcagtgg cttagttcgt tgcgtaatac agtaacagaa ttaaaagtgg ttttgcccaa
    19321 gtgtaattta gcactgagta aaaagttaga acttgatctc cataaatatc gctcagatgt
    19381 caagattatt ttaaaatatt ctgaagggaa taattatata ttctgtagtg gtaatcaagt
    19441 agtggagttt agtcctcagg atgtaatatc aggtgttaaa atagaacttg cggaaaaatt
    19501 aacaaaaaat gataaaaaat atttcaaaga gttagcacat aagaaactga gacaaattgc
    19561 agaagacttg ttaaaagaga atcctgttaa tgattaatca aataaatgca agcaatgctc
    19621 tgcagcaaag attaaatagt gaagaatttg ttaatttaaa tgagagatta tcatcttcgc
    19681 aatcatttga tgaggatata atctatgaaa taatgcaata tttctcacag agtgaattaa
    19741 attccataga taatgatgaa ctgcacaata agattgaaca attattcaat tcacgttttc
    19801 cttatcttac agctgcacaa aaaagttcac ttcttaataa actcatagat gcaaatcaat
    19861 atgtcgattt gcatgaggga ttttatgcat cattgtccat atataacaat attgatttct
    19921 atattaagac tacaaccttc gattcattaa ttagtgtctt tgaagcaggg agagaggcag
    19981 atgattcgac atggtagtaa taagttgaaa atatttattt taagtatatt gctattaaca
    20041 ctgagtgggt gtgctttaaa gtcatcatct aattctgaaa aagaatggca tattgttcct
    20101 gtaagtaagg attatttttc tattccaaat gatttattat ggtcgtttaa tacaaccaat
    20161 aaaagtataa atgtttactc taaatgtatt agtggtaagg cggtttatag ttttaatgca
    20221 ggtaaattca tgggcaactt taatgttaag gaagtagatg ggtgcttcat ggatgcacaa
    20281 aagatagcta tagataaact attttctatg ctgaaagacg gggttgtttt aaaaggtaat
    20341 aagataaatg ataccatcct tatagagaag gatggggaag ttaaattaaa attaattcga
    20401 gggatataat tgtattgtga gtaaatataa aggtctaaat acaagtaata tgttttacat
    20461 ttactctagt ggacatgaac cagttaacgt tgagcttgta aaagataaag aacgtaacat
    20521 aattgagctg gctccagcat ggaagggttt ttttttgtcc gaaatcaaaa tataaaattc
    20581 agtgataatg tgaactatca ttatcgcttt aacattaata gttgtgcaaa atttcttgct
    20641 ttttgggact atttcagtgg tgctctagta gagcatagcc atgctgaaaa atgtattcat
    20701 ttctatcatg agaatgatct tcgggatagt tgtaataccg aatcgatgct tgataagtta
    20761 atgttaagat tcatcttttc atctgaccag aatgtaagta atgcactggc tatgatacga
    20821 atgactgagt catatcattt ggttctttat cttctgagaa ctattgagaa agaaaaggag
    20881 gtaagaataa agagtctgac agagcattat ggtgtgtcgg aggcttattt tagatcttta
    20941 tgtagaaaag ctttaggggc aaaagtcaaa gaacaactga acacttggcg gttagtaaat
    21001 ggtttattag atgtattttt gcacaatcaa acaatcactt ctgctgccat gaataatggg
    21061 tatgcatcaa catcccattt ttcaaatgag ataaaaacac gactaggctt ttctgcaaga
    21121 gaattatcga atattacttt tttagtgaaa aaaataaatg aaaaaattta atattaaatc
    21181 tttgactctc ttgattgtat tgttaccatt gatagttaat gctaataata ttgactctca
    21241 tttattagaa caaaatgaca tagcgaaata tgttgctcaa agtgatacgg taggatcttt
    21301 ttttgaacgt ttttctgcgt tattaaatta tcctattgtt gttagcaaac aagctgcaaa
    21361 aaagagaata tctggggagt ttgatttatc taatccagaa gaaatgcttg aaaaactgac
    21421 attacttgtg ggactcatat ggtataaaga tggaaatgca ttatacatat atgactctgg
    21481 agaattaata agcaaagtta ttttgctgga gaatatatca cttaattatc ttattcagta
    21541 tctgaaggat gctaaccttt atgatcacag atatcctata agaggaaaca ttagtgataa
    21601 aacattctat atatctggtc ctccggcatt ggttgaactg gtagctaata cagcaacttt
    21661 gcttgataag caggtttctt ctattgggac agataaagtt aattttgggg ttataaaatt
    21721 aaaaaataca tttgttagtg acagaactta taatatgaga ggggaggata ttgtcatccc
    21781 aggggtggct actgttgtag aacgtttatt aaataatggt aaggctctga gtaacagaca
    21841 agcccaaaat gatccgatgc caccctttaa tattacacag aaagtatctg aggatagtaa
    21901 tgacttttca tttagttcag ttaccaatag ttctatatta gaggatgtat cacttattgc
    21961 atatccagaa accaattcta tattagtcaa aggtaatgat cagcaaatac agattattcg
    22021 cgatattatc actcagttag atgtagccaa acgacatata gaattatcat tatggataat
    22081 cgatattgat aaatctgaat taaataacct tggtgttaat tggcaaggaa cggcttcatt
    22141 tggtgattcg tttggtgcgt catttaatat gagctcatca gcatcgataa gcactttgga
    22201 cggcaataag tttattgctt cagttatggc attaaatcag aaaaagaaag caaatgtagt
    22261 ttcaaggccg gtgattttga cacaagagaa tattccggct atatttgata ataacaggac
    22321 tttttatgtg tcattagttg gtgagcgcaa ttcttcgttg gagcatgtta cgtatggaac
    22381 attgataaat gttatacctc gcttttcatc tcggggacaa attgagatgt ctttaacgat
    22441 agaggatgga acaggaaata gtcagtcaaa ttacaattat aataatgaaa atacatctgt
    22501 ccttccagaa gttggaagga ccaaaatcag tacaatagcg agagttccac aaggcaaaag
    22561 tttgttgata ggtggttata cgcatgaaac gaactctaac gaaatagtat ctattccttt
    22621 tctctcctct attcctgtaa tcggtaatgt gttcaagtat aaaacaagta atatcagtaa
    22681 tattgtaagg gtttttctga ttcaaccccg tgagataaag gagtcaagtt attataatac
    22741 tgcagaatat aaaagcctca tatctgaaag agaaatacaa aagactacac aaataatccc
    22801 atctgaaact actttgctgg aagacgaaaa atcattggtt tcatacttaa attactaact
    22861 ataaagtagg tgatgtatgc ttgatgttaa aaatacagga gtttttagct ctgcattcat
    22921 tgataagttg aatgcaatga caaattcaga tgatggagat gagactgctg atgcagagct
    22981 tgattctggc ttggctaata gcaagtatat tgactcatct gatgagatgg cttccgctct
    23041 ttcgtcattt ataaacagaa gagaccttga gaaactgaaa ggaacaaata gtgatagtca
    23101 ggaacgtatt ttagatgggg aagaagatga aattaatcac aagatttttg atttaaagag
    23161 aacgttaaaa gataaccttc ctctagatcg ggattttata gacagactaa agagatattt
    23221 taaagatcca agtgatcaag tcttagcatt aagggaactt ttgaatgaaa aagatcttac
    23281 tgctgaacaa gtcgaattat taactaaaat tattaatgag ataatatcag gtagtgaaaa
    23341 aagtgttaat gctggaataa attcagctat acaggctaaa ttatttggca acaaaatgaa
    23401 acttgaacca cagcttttgc gtgcatgtta tcgtggtttt atcatgggga acatatcaac
    23461 aacagatcag tatatagaat ggcttggtaa ttttggtttt aatcacagac atacaattgt
    23521 gaattttgta gagcagtcac tgattgtaga catggattct gagaaaccga gctgtaatgc
    23581 ttatgagttt ggttttgtgt tatctaaatt aattgcaatt aagatgatta gaacttcaga
    23641 cgtaattttt atgaagaaac tggaatcctc aagcttgcta aaagatggca gtttaagtgc
    23701 agagcagcta ttgctaactt tattatatat ttttcaatat ccaagtgaaa gtgagcaaat
    23761 tcttacttct gttatagaag tatcacaagc cagtcatgag gattctgtag tgtatcaaac
    23821 atatctatct tctgttaatg aaagtcctca tgatatattt aaaagtgaaa gtgaaagaga
    23881 aattgcgatc aatattctac gagagcttgt cacaagtgca tacaagaaag agctttctag
    23941 ataacaggag ataaaagtga tccagtcttt tcttaagcaa gtaagtacta agcctgaatt
    24001 aattatatta gtgcttatgg ttatgatcat tgctatgttg atcataccat tacctacata
    24061 ccttgttgat tttttaatag ggcttaatat tgtacttgct attctcgttt ttatgggatc
    24121 attttatatt gaaagaatat tgagtttttc tacattccct tcggttttgt tgataacgac
    24181 attatttcgt ctcgctttat caataagtac tagtcgacta attttagttg atgcggatgc
    24241 gggtaaaatt attactacat ttggccagtt tgtcattggt gatagtctgg ctgtaggttt
    24301 tgttattttt tcaatagtaa ctgttgtgca gttcattgtt ataacaaaag gatctgaaag
    24361 ggttgcggag gtagctgctc gcttttctct tgacggtatg ccggggaaac aaatgagcat
    24421 tgatgcggat ttaaaggctg gaattattga tgccgcagga gctaaagaaa gacgcagtat
    24481 tctggaacgt gagagtcaat tgtatggttc atttgatgga gcaatgaagt tcattaaagg
    24541 tgatgcaatt gctggcatca ttattatttt tgtcaattta ataggaggta tttctgttgg
    24601 tatgagtcaa catggaatgt ccctatctgg tgcattatct acttatacca tactaactat
    24661 tggtgacgga ttggtctctc agattcctgc tctgttaatt tctataagtg caggatttat
    24721 cgttacaaga gttaatggtg atagcgataa tatgggacgt aacataatgt cccagatatt
    24781 tgggaatcct tttgttctca ttgttacatc agctcttgcc ttggcgatag ggatgttgcc
    24841 aggttttccg ttttttgttt ttttcctgat agcagttact ttgacggctt tattctatta
    24901 taaaaaggtc gtagaaaaag aaaaaagtct gtctgagtct gattctagtg gctatactgg
    24961 tacatttgat atcgataata gccatgactc atctttggca atgatagaaa atctggatgc
    25021 tattagttca gaaaccgttc ctttaatttt attatttgcc gaaaataaga taaatgcaaa
    25081 tgatatggaa ggtcttattg aaaggataag aagtcagttc tttattgatt atggtgtcag
    25141 gcttccaact attttatata gaacaagcaa tgagcttaag gtcgatgata ttgttttgtt
    25201 aataaatgag gtgcgtgccg atagttttaa tatatatttt gacaaagtgt gtattacaga
    25261 tgaaaatgga gatatagatg ctctaggtat tcctgttgtt tcaacttcat ataatgaacg
    25321 tgttatttct tgggtagacg tttcatatac agaaaatcta actaatattg atgctaaaat
    25381 taaaagtgct caagatgaat tttatcacca gttgtcacaa gctttattaa acaacataaa
    25441 tgagattttt ggtatacaag aaacaaaaaa tatgttagat cagtttgaaa atcggtatcc
    25501 tgatctatta aaggaagtct tccgacatgt gactatacag agaatttctg aggtattaca
    25561 aagattgctt ggagaaaata tttctgttcg caatttaaaa cttattatgg agtctttggc
    25621 gctttgggct cccagagaaa aagatgtcat aacattagtt gaacatgtcc gtgcatcact
    25681 ttctaggtat atttgtagta aaatagctgt ttctggtgag attaaagttg tgatgctttc
    25741 cggatatatt gaggatgcaa taagaaaagg gataaggcaa acctctggtg gctctttctt
    25801 gaatatggat atagaggttt cggatgaggt aatggaaact ttagcacatg ctttgagaga
    25861 attgagaaat gcaaaaaaaa atttcgttct tttggtatca gtagatatac gtaggtttgt
    25921 taaaagactt atagataaca gatttaagag tatactcgtt atatcgtatg ctgagattga
    25981 tgaagcatat accattaatg tattaaagac tatttagtga ggtttaaata tgagtaacat
    26041 taatttagtt caattagtta gagatagtct tttcacgatt ggttgtcctc catcaattat
    26101 tacagattta gacagccatt cagcgattac catttctttg gatagtatgc ctgctattaa
    26161 tatagctctt gtcaatgagc aggttatgtt atgggctaat tttgatgcac caagtgatgt
    26221 aaaacttcag tcatctgctt ataacatatt aaatttaatg ttaatgaact ttagttatag
    26281 cattaatgag ctggtagagc ttcatcgttc tgatgagtac ttacagttaa gagttgttat
    26341 taaagatgac tacgttcacg atgggattgt ttttgcagag attctgcatg agttttatca
    26401 gaggatggaa atcttaaatg aggtcttata atcaatgagc tatacaaaat tgctcactca
    26461 attatctttt cctaatagaa tctcggggcc aatcttggaa acaagtctta gcgatgtttc
    26521 gattggtgag atttgtaaca ttcaggctgg aattgaaagt aatgaaattg ttgcaagagc
    26581 tcaggttgta ggatttcatg atgaaaaaac aatattaagc ttgattggaa attctcgtgg
    26641 actttcacgg caaacgttga ttaagcccac tgcccagttt cttcatacgc aagtcggccg
    26701 tggattattg ggagcagtag tcaatccttt aggggaggtt actgataagt ttgctgttac
    26761 ggataacagt gaaattcttt atcgacctgt agataatgct cctccgctat atagtgaaag
    26821 ggctgcaatt gagaagcctt ttttaacagg tattaaggtt attgattctt tactcacgtg
    26881 tggtgaagga cagcgaatgg ggatttttgc gtcagctggt tgtggcaaaa cttttctcat
    26941 gaatatgctc attgaacata gtggtgctga tatatatgtt attgggttaa ttggtgagcg
    27001 aggtcgagag gttacagaaa cggttgatta tttgaaaaac tctgagaaaa aaagcaggtg
    27061 tgttttagta tatgcaactt cggattactc ttcggttgat cgttgtaatg ctgcatatat
    27121 agccactgct atagccgaat tttttaggac tgaaggacat aaagtagcgc tttttattga
    27181 ttcattaaca aggtatgcca gagcattacg tgatgtggcc ttagccgctg gagaatcacc
    27241 tgccagaaga ggctatccgg tttcggtttt tgatagctta cccagacttc ttgaaaggcc
    27301 aggaaagtta aaggcaggtg gctctattac tgcattttac actgttcttt tggaggatga
    27361 tgattttgct gatccattag ctgaagaggt aagatccatt ttagatggac atatatattt
    27421 gagcagaaat ctagcccaaa aaggacaatt tcctgcaatt gattccttaa aaagtataag
    27481 cagggtattt acacaggttg ttgatgaaaa acatcgtatt atggccgctg catttcggga
    27541 gctactttct gaaatagaag agctaaggac aattattgac tttggtgaat acaaaccggg
    27601 ggagaatgcc tctcaggata aaatctacaa caaaatatct gttgttgaga gttttctgaa
    27661 gcaagattat cgactcggtt ttacttatga gcagacaatg gagcttattg gtgaaacaat
    27721 tagataaggt tttaaaaatc aaagataaat atcaacggtc tgttaaatta attgaagcac
    27781 acatattaac actaaaaaaa aaactctttg tatcgagatg tggaggcatt agataaaagg
    27841 attatatact tcctgcaact ggagaatgac ttagaacctg taggcgctca gagcgttagt
    27901 cagcttttca atacaagacg taagatcgct atagtaaaaa agcatattat ccaatatcag
    27961 tctgaacgaa ttcttctaaa gggaagaatt gaagaaattc aaaaagatat tgatgaggca
    28021 aatgcttcta aaagaaaact gcttcataaa gagagtaaaa tatgtaaacg gattggcttg
    28081 attaaaagaa ataactttgc caaacagttg atacttgatg agttatcaca agaggatatg
    28141 aagtatggca ttagataata taaacctaaa ttttagtagt gacaaacaga tagaaaaatg
    28201 tgagaaacta tcttcaatag ataatattga tagtctcgtt ttgaagaaga agaggaaggt
    28261 tgaaattcct gagtactctt taattgcatc taactatttt actattgata agcactttga
    28321 acataagcat gataaaggag aaatttatag tggcattaaa aatgcgttcg aacttagaaa
    28381 cgaacgagcg acatattctg atattccaga atcaatggcc attaaagaaa atattttgat
    28441 accagatcaa gatatcaaag caagggaaaa aataaatatc ggcgatatga gggggatctt
    28501 ttcatataat aagagtggaa atgcagacaa gaacttcgaa agaagtcata cttcttctgt
    28561 aaaccctgat aatctgctag aatctgataa tagaaatggt caaattggtt taaaaaatca
    28621 tagcttgtct attgataaga atattgctga catcatttct ttactaaatg gaagtgttgc
    28681 taaatcattt gagctgcctg taatgaataa aaatactgca gacataaccc catccatgtc
    28741 attgcaagaa aaatcaatag ttgaaaatga taaaaatgtt tttcaaaaaa atagtgaaat
    28801 gacttaccac tttaaacagt ggggggctgg acattctgtt agtatttcag tggagtctgg
    28861 ttcttttgtt ctaaaaccgt cagatcaatt tgtaggaaat aaacttgact taattttgaa
    28921 acaagatgct gagggtaatt acagatttga tagcagtcaa cataataagg ggaataaaaa
    28981 taatagtaca ggatataatg aacagagtga agaagaatgc taagaattaa acattttgac
    29041 gctaacgaaa aactacagat tttatatgca aagcaactct gcgagcgttt ttcgattcag
    29101 acattcaaaa ataaatttac aggcagtgaa agtttagtca ctcttacttc cgtgtgtggg
    29161 gattgggtaa ttcgtattga tacattatct tttttgaaaa aaaaatacga ggtattttca
    29221 ggattttcta cacaagaatc tttactgcat ttatcaaaat gtgtctttat agagtcgtca
    29281 tctgtatttt cgattccaga actgtctgat aagattactt tccggatcac gaatgaaatc
    29341 cagtatgcaa ctactgggag tcatttatgc tgtttttcct cttctttagg tattatttat
    29401 tttgacaaga tgccggtatt acgtaatcaa gtttctcttg acttattgca tcatctttta
    29461 gagttttgct taggttcatc taatgtaagg ctggctactt taaaaagaat tcgcactggt
    29521 gatataatca tagttcagaa actttataat ttattattgt gtaatcaagt tattattggg
    29581 gattatattg tgaatgataa taatgaggca aaaattaatc tgtcagaaag taatggtgag
    29641 tcagaacaca cagaagtttc tttggcatta ttcaattatg atgatatcaa tgtaaaagtg
    29701 gactttattc ttttagaaaa aaatatgaca atcaatgaac taaaaatgta tgtagaaaac
    29761 gaattattca agtttcccga tgacatagtt aaacatgtaa atattaaagt aaatggttct
    29821 ttggttgggc atggggaact tgtttctatt gaggatggtt atggtatcga gattagttct
    29881 tggatggtaa aggagtaatc tcatgctgag tgacatgtcc ctcatagcta cactttcttt
    29941 ttttacgcta ttaccatttt tagttgctgc tggtacctgt tatataaagt tttctattgt
    30001 ttttgtaatg gtacgaaatg cacttggttt gcagcaggtt ccatcaaata tgactctcaa
    30061 tggtattgca ttgattatgg ctttgttcgt gatgaaaccc attattgaag ctggttatga
    30121 aaattatcta aatggccctc aaaaatttga tacaatatct gatattgtcc gtttttctga
    30181 ttctggcttg atggaatata agcaatactt gaaaaagcat acagatcttg agttagcacg
    30241 ttttttccaa aggtctgaag aagaaaatgc tgatttaaag agtgcagaga ataatgatta
    30301 ttcattgttt tccctcttgc ctgcatatgc gctaagtgaa atcaaagatg cgttcaaaat
    30361 aggtttttat ttatatttgc catttgttgt tgttgatctt gttatttcaa gcattttact
    30421 tgcacttggt atgatgatga tgagtccaat cacaatatca gtaccaataa aacttgtatt
    30481 atttgttgcg cttgatgggt ggggtatcct gtctaaggcc ttgattgaac aatatatcaa
    30541 tattcctgct tagatgatgg aggtaatgtg tctgatatag tttatatggg taataaggct
    30601 ctttatttaa tccttatctt ttccttatgg ccagtaggta tagctacggt tattggatta
    30661 tctattggtt tgttacagac agtgactcaa ctccaagagc agacacttcc ttttggtata
    30721 aagcttatag gtgtatcaat atctttgcta cttctttctg gatggtatgg tgaggtttta
    30781 ttgtcttttt gtcatgaaat aatgttttta attaagagtg gggtttgatg gacatttcaa
    30841 gctggttcga aagtattcat gtgtttttaa tactcctgaa cggcgttttt tttagattgg
    30901 ctccattatt tttctttctt ccatttttaa ataacggtat aatttctcca tctattagaa
    30961 tacctgtgat ttttcttgtt gcatcaggat taattacttc tggtaaggta gacataggtt
    31021 cttctgtttt tgaacatgtt tatttcctta tgttcaagga aataattgtt ggcctccttc
    31081 tctctttttg cttgtctctt cccttttgga tatttcatgc tgttggtagc attattgaca
    31141 accagcgtgg ggcaacgctt agtagttcaa ttgatcctgc caatggtgtt gatacgtctg
    31201 agttggcaaa atttttcaat cttttttctg cagttgtatt tctatacagt ggtggtatgg
    31261 tctttatttt agaatccata caattgtctt ataatatatg cccgttattt tctcaatgtt
    31321 ctttccgtgt ctcaaatatc ttaacatttc tgactttatt ggcaagtcag gctgttattt
    31381 tagccagtcc tgttatgata gtattgttac tatcagaagt attacttggt gtattatcga
    31441 gatttgctcc gcagatgaat gctttttccg tatcattaac tattaaaagt ttacttgcaa
    31501 tatttattat tttcatctgt tcttctacta tttacttttc taaagttcaa tttttcctcg
    31561 gtgaacataa atttttcaca aatctatttg ttagataaaa tattatggca aataaaacag
    31621 aaaagccgac acctaaaaaa ctaaaggatg ccgcaaaaaa aggacagtca tttaaattta
    31681 aggatttaac gactgttgtt attattctgg tagggacatt tactataata tcattctttt
    31741 ccttaagtga tgtaatgctc ttatacagat atgtaataat taatgacttc gaaattaatg
    31801 agggtaaata cttttttgca gtggttattg tcttttttaa gataattggc ttcccacttt
    31861 ttttctgtgt tctttcggct gtgttgccaa cattggttca aacaaagttt gttcttgcga
    31921 ctaaagctat caagattgat ttttcagtat taaaccctgt taaagggtta aaaaaaatat
    31981 ttagtataaa gacaataaaa gaatttttca aaagcattct gcttcttatt attctagcat
    32041 taacaaccta tttcttttgg attaatgacc gaaaaataat tttttctcag gtgttttcta
    32101 gtgttgatgg cttatatctt atttgggggg ggctgtttaa ggatataata ttatttttct
    32161 tggcattttc tatttttgtt attattcttg actttgtgat tgagttcatt ttatacatga
    32221 aagatatgat gatggataaa caggagataa aaagagaata tatagagcaa gagggacact
    32281 ttgagacaaa gtcgagaagg cgtgagttgc atatcgagat tctttcagag cagactaaat
    32341 ctgatatacg taattcaaaa ttagtggtaa tgaacccgac tcatattgca attggtattt
    32401 attttaatcc agaaatagcg cctgcacctt ttatttctct cattgaaact aaccagtgtg
    32461 ccttggctgt cagaaaatat gcaaatgaag ttggtatacc gactgtgcgt gatgtgaaat
    32521 tagctagaaa actatataaa acacatacaa aatatagttt tgttgatttt gaacacttgg
    32581 atgaagtcct acgtcttatt gtttggcttg agcaggttga aaacactcat taataagtaa
    32641 aggagatgtg ttatatggga gttaatttct gtaataaaat aggtattgat cagtctgaat
    32701 ttgaaataga atcttccatc attaactcca ttgctaatga ggtattgaac ccaatatctt
    32761 tccttagcaa taaggatata ataaatgttt tactcagaaa gatttcatct gaatgtgacc
    32821 tggttagaaa agacatttat cgttgtgctc tggagttggt cgttgaaaaa acacctgatg
    32881 atttataatc attcataata actcttttgc tgatataaat atttttgttg aagatattta
    32941 tgtagtcaca ggatcaattg tagaaaattg ttatgttttt gttaggaact gcactaaata
    33001 atatatgata agacagcaaa aacgactgac cataatcctt cttttattag gagttgataa
    33061 gagagactat tcatcctgta atgttaaaac tctgttatat agtattcgcg attatgcaaa
    33121 gtctgtaaat gatcatgaga tactaaccga gagtaatcgt ttattatctc attgcatttc
    33181 agatagtaac ggagctttct ttaaaagtag taaatacgtt ccccttaagt acttacgtaa
    33241 aaggaaaata gcacgaaaga taccaaatga ttagcacttt gctagtaagc ttgctggtaa
    33301 actatattga ccgttgggtt tgttttgaga ctgtaattta aattgtgtaa ttgcctgttt
    33361 ttgatatgtt tactccaaca acggagacag gtaaattatg gacgaaaaga agcttaaagc
    33421 ccttgcggct gaactggcta aaggcctcaa aactgaggcc gacctcaatc agttttcccg
    33481 tatgctgacg aaatatcccg gctcttaaaa cgatagtggc tcaggctttc atttttcagc
    33541 gttccccaga ag
//
LOCUS       DADWTH010000038        33044 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.038, whole genome shotgun sequence.
ACCESSION   DADWTH010000038 DADWTH010000000
VERSION     DADWTH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.038"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            14..4591
                     /locus_tag="JNY02_001895"
     CDS             14..4591
                     /locus_tag="JNY02_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HBB9433445.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDA
                     SVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANAD
                     YDNVGDALNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSD
                     AINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALL
                     WDKTANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQII
                     NQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAI
                     GQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDD
                     GKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAM
                     GAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGY
                     TAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSI
                     TNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIA
                     AADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVR
                     YDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQR
                     MVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSM
                     VSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            4959..6614
                     /gene="lldP"
                     /locus_tag="JNY02_001896"
     CDS             4959..6614
                     /gene="lldP"
                     /locus_tag="JNY02_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBB9433446.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6614..7390
                     /gene="lldR"
                     /locus_tag="JNY02_001897"
     CDS             6614..7390
                     /gene="lldR"
                     /locus_tag="JNY02_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBB9433447.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7387..8577
                     /gene="lldD"
                     /locus_tag="JNY02_001898"
     CDS             7387..8577
                     /gene="lldD"
                     /locus_tag="JNY02_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBB9433448.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8773..9246
                     /gene="trmL"
                     /locus_tag="JNY02_001899"
     CDS             8773..9246
                     /gene="trmL"
                     /locus_tag="JNY02_001899"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBB9433449.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9299..10120)
                     /gene="cysE"
                     /locus_tag="JNY02_001900"
     CDS             complement(9299..10120)
                     /gene="cysE"
                     /locus_tag="JNY02_001900"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBB9433450.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10200..11219)
                     /gene="gpsA"
                     /locus_tag="JNY02_001901"
     CDS             complement(10200..11219)
                     /gene="gpsA"
                     /locus_tag="JNY02_001901"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBB9433451.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11219..11686)
                     /gene="secB"
                     /locus_tag="JNY02_001902"
     CDS             complement(11219..11686)
                     /gene="secB"
                     /locus_tag="JNY02_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBB9433452.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11749..12000)
                     /gene="grxC"
                     /locus_tag="JNY02_001903"
     CDS             complement(11749..12000)
                     /gene="grxC"
                     /locus_tag="JNY02_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBB9433453.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12142..12573)
                     /locus_tag="JNY02_001904"
     CDS             complement(12142..12573)
                     /locus_tag="JNY02_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB9433454.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12818..14362
                     /gene="gpmM"
                     /locus_tag="JNY02_001905"
                     /gene_synonym="pgmI"
     CDS             12818..14362
                     /gene="gpmM"
                     /locus_tag="JNY02_001905"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBB9433455.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14372..15655
                     /gene="envC"
                     /locus_tag="JNY02_001906"
     CDS             14372..15655
                     /gene="envC"
                     /locus_tag="JNY02_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBB9433456.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15659..16618
                     /locus_tag="JNY02_001907"
     CDS             15659..16618
                     /locus_tag="JNY02_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBB9433457.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16605..17639)
                     /gene="waaH"
                     /locus_tag="JNY02_001908"
     CDS             complement(16605..17639)
                     /gene="waaH"
                     /locus_tag="JNY02_001908"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBB9433458.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17878..18903)
                     /gene="tdh"
                     /locus_tag="JNY02_001909"
     CDS             complement(17878..18903)
                     /gene="tdh"
                     /locus_tag="JNY02_001909"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBB9433459.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18913..20109)
                     /gene="kbl"
                     /locus_tag="JNY02_001910"
     CDS             complement(18913..20109)
                     /gene="kbl"
                     /locus_tag="JNY02_001910"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBB9433460.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20384..21241)
                     /gene="yibB"
                     /locus_tag="JNY02_001911"
     CDS             complement(20384..21241)
                     /gene="yibB"
                     /locus_tag="JNY02_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBB9433461.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21546..22478
                     /gene="rfaD"
                     /locus_tag="JNY02_001912"
     CDS             21546..22478
                     /gene="rfaD"
                     /locus_tag="JNY02_001912"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBB9433462.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22488..23534
                     /gene="rfaF"
                     /locus_tag="JNY02_001913"
     CDS             22488..23534
                     /gene="rfaF"
                     /locus_tag="JNY02_001913"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBB9433463.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23538..24530
                     /gene="rfaC"
                     /locus_tag="JNY02_001914"
     CDS             23538..24530
                     /gene="rfaC"
                     /locus_tag="JNY02_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBB9433464.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLDYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24527..25735
                     /gene="rfaL"
                     /locus_tag="JNY02_001915"
     CDS             24527..25735
                     /gene="rfaL"
                     /locus_tag="JNY02_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBB9433465.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25771..26913)
                     /locus_tag="JNY02_001916"
     CDS             complement(25771..26913)
                     /locus_tag="JNY02_001916"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBB9433466.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26922..27935)
                     /locus_tag="JNY02_001917"
     CDS             complement(26922..27935)
                     /locus_tag="JNY02_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HBB9433467.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27960..28667)
                     /gene="rfaY"
                     /locus_tag="JNY02_001918"
     CDS             complement(27960..28667)
                     /gene="rfaY"
                     /locus_tag="JNY02_001918"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBB9433468.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28693..29700)
                     /gene="waaO"
                     /locus_tag="JNY02_001919"
                     /gene_synonym="rfaI"
     CDS             complement(28693..29700)
                     /gene="waaO"
                     /locus_tag="JNY02_001919"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBB9433469.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29743..30540)
                     /gene="rfaP"
                     /locus_tag="JNY02_001920"
     CDS             complement(29743..30540)
                     /gene="rfaP"
                     /locus_tag="JNY02_001920"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBB9433470.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30533..31657)
                     /locus_tag="JNY02_001921"
     CDS             complement(30533..31657)
                     /locus_tag="JNY02_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB9433471.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31654..32712)
                     /gene="rfaQ"
                     /locus_tag="JNY02_001922"
     CDS             complement(31654..32712)
                     /gene="rfaQ"
                     /locus_tag="JNY02_001922"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBB9433472.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8732 a         8085 c         7801 g         8426 t
ORIGIN      
        1 gcgagcatca ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta
       61 tactgttacc agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt
      121 aatttctgcg ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg
      181 gaatgacaac ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc
      241 tataggcaaa ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt
      301 gggttatgac gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc
      361 gattggtggt gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat
      421 agcactgggt gcctcttcgt attcattggg ccaatactca atggccctgg ggcgtagcag
      481 tgtagctagc ggtactgaca gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa
      541 cgctattgcg ataggtgctg agaccgaagc cgctgaaaat gcaactgcta ttggcaataa
      601 tgcgaaggca aaagggacta atagcatggc aatggggttc ggaagccttg ccgataaagt
      661 caatactatc gcattaggaa atggcagcca ggctctggca gataatgcaa tcgccatagg
      721 ccagggcaac aaagctgatg gcgtggatgc catcgctctg ggtaatggta gccagtcgag
      781 aggcttaaac accattgcct taggcacagc cagtaatgca actggtgata agagtcttgc
      841 gcttggtagt aatagcagtg ccaacggtat taactctgtc gcgctgggcg cagattccat
      901 tgcggattta gacaataccg tctctgtcgg caatagttca ttaaaacgca agatcgttaa
      961 tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc attaatggtt cacagcttta
     1021 tgccattagc gactcggtag caaaaaggct tggaggaggg gctgcagtag atgttgatga
     1081 cggtactgtt acagcaccaa cctacaattt aaaaaatggt agcaaaaata acgtaggggc
     1141 tgcgctcgct gtacttgatg aaaacaccct gcaatgggac caaaccaaag gcaaatacag
     1201 cgctgctcat ggtactagta gcccaactgc cagcgtaatc accgatgttg cggatggcac
     1261 gatttcagcc tccagtaagg atgcggttaa cggttcccaa ctgaaagcta ccaatgacga
     1321 tgtcgaagcc aacaccacca atatcaccaa cctgacggat tccgttggtg accttcaggc
     1381 tgatgccctg ctctggaacg aaactaaaaa ggcattcagt gcagctcacg gccaggatac
     1441 caccagcaaa ataaccaacg ttaaagatgc cgacctgacg gctgacagca ctgatgctgt
     1501 taacggctct cagctgaaaa ccaccaacga tgctgtggcg acgaatacca ccaatatcgc
     1561 caataacact tccaatattg ccactaacac caccaacatc tctaacctga ctgagacggt
     1621 gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtat tcacggcagc
     1681 tcatggcacc gagaccacca gcaaaataac caacgttaaa gatggcgacc tgacgactgg
     1741 cagcaccgat gccgttaacg gctctcagct gaaaaccacc aacgatgccg tggcgacgaa
     1801 taccaccaat atcgccacta acaccaccaa catctctaat ctgactgaga cggtgactaa
     1861 tcttggtgag gatgcgctga aatgggataa ggacaatggt gtcttcactg cagctcatgg
     1921 caacaatacc gccagcaaaa tcaccaatat cctggacggc acagtcactg caaccagttc
     1981 cgatgccatt aacggtagcc agctttatga cttaagcagc aatatcgcca cctacttcgg
     2041 cggcaatgct tctgtgaata ctgacggcgt gtttaccggt ccaacctaca aaatcggtga
     2101 aacaaattat tataacgtcg gcgatgcact ggctgcgatt aactcctcat ttagcacgtc
     2161 tctcggcgat gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca aacacggtac
     2221 taatggtgac gcaagcgtga tcactgatgt cgcagatggt gaaatttcag actccagttc
     2281 tgacgcagta aacggctcac aactccacgg cgtgagcagt tatgttgttg atgcgctggg
     2341 gggtggtgcc gaagtcaatg cagacggcac catcactgcg ccgacgtaca ccattgctaa
     2401 tgctgattac gataatgtcg gtgatgccct gaatgctatc gataccactc ctgacgacgc
     2461 tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga
     2521 taaaactgcc agtgtaatca ctaacgtcgc taacggtgca atctctgctg ccagcagcga
     2581 cgcgattaac ggctcacaac tctataccac caataagtac atcgctgatg cgctgggtgg
     2641 tgacgcagaa gtcaacgctg acggcaccat caccgcaccg acttacacca ttgcgaacgc
     2701 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
     2761 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
     2821 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
     2881 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
     2941 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
     3001 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
     3061 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
     3121 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
     3181 aggttctcgt gacactagcg taacggaaaa tggcttggtg attggttatg acaccactga
     3241 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
     3301 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
     3361 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
     3421 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
     3481 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
     3541 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
     3601 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
     3661 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
     3721 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
     3781 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
     3841 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
     3901 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
     3961 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
     4021 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
     4081 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
     4141 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
     4201 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
     4261 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
     4321 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
     4381 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
     4441 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
     4501 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
     4561 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
     4621 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
     4681 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
     4741 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
     4801 aactcacatc aatattgcta atacataaca tttagttaac cattcattgt cattatccct
     4861 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
     4921 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
     4981 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
     5041 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
     5101 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
     5161 cggtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
     5221 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
     5281 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
     5341 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
     5401 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
     5461 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
     5521 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
     5581 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
     5641 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
     5701 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
     5761 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatataa
     5821 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
     5881 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
     5941 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
     6001 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
     6061 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
     6121 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
     6181 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
     6241 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
     6301 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
     6361 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
     6421 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
     6481 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
     6541 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
     6601 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
     6661 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
     6721 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
     6781 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
     6841 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
     6901 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
     6961 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
     7021 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
     7081 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
     7141 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
     7201 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
     7261 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
     7321 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
     7381 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
     7441 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
     7501 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
     7561 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
     7621 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
     7681 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
     7741 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
     7801 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
     7861 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
     7921 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
     7981 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
     8041 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
     8101 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
     8161 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
     8221 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
     8281 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
     8341 attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca
     8401 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
     8461 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
     8521 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
     8581 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
     8641 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
     8701 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
     8761 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
     8821 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
     8881 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
     8941 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
     9001 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
     9061 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
     9121 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
     9181 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
     9241 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
     9301 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
     9361 ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg
     9421 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
     9481 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
     9541 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
     9601 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
     9661 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
     9721 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
     9781 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
     9841 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
     9901 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
     9961 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    10021 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    10081 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    10141 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    10201 tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    10261 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    10321 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    10381 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg
    10441 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    10501 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    10561 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    10621 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    10681 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    10741 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    10801 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    10861 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    10921 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    10981 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    11041 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    11101 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    11161 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    11221 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    11281 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    11341 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    11401 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    11461 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    11521 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    11581 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    11641 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    11701 taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag
    11761 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    11821 tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg
    11881 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    11941 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    12001 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    12061 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    12121 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    12181 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    12241 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    12301 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    12361 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    12421 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    12481 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    12541 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc aacgtagtga
    12601 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    12661 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    12721 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    12781 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    12841 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    12901 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    12961 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    13021 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    13081 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    13141 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    13201 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    13261 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    13321 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    13381 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    13441 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    13501 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    13561 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    13621 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    13681 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    13741 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    13801 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    13861 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    13921 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    13981 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    14041 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    14101 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    14161 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    14221 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    14281 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    14341 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    14401 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    14461 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    14521 tattcaggcc gatatcgccg caaaagagca cgcggtacgc caaaagcaac aacaacgcgc
    14581 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    14641 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    14701 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    14761 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    14821 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    14881 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    14941 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    15001 tcaggttctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    15061 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    15121 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    15181 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    15241 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    15301 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    15361 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    15421 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    15481 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    15541 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    15601 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    15661 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    15721 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    15781 ccaagtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    15841 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    15901 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    15961 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    16021 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    16081 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    16141 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    16201 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    16261 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    16321 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    16381 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    16441 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    16501 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    16561 gtggcaaggc tggggcaaac agcacgaagc ggcgaagttt aacgctagcg caaattaaag
    16621 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    16681 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    16741 tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat atccggctct
    16801 ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg aaattccgga
    16861 taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg ggtaatctta
    16921 atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg actcactgac
    16981 gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg cagcgcgtta
    17041 aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat aaatttaatg
    17101 ttatttttaa caataacatc acggcgataa acccccatcc agacaacgtg agtccaacgg
    17161 cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacaccggt tgagcgaagg
    17221 cgatcgctgg ggatggattg ccaggtttct cccgtttcac gaaaagacca gtcagcgttg
    17281 cactgcgcca cgtcgaggtc gtcctctaac gccatggtca tcagcgtttc gtacatggtg
    17341 ggatagacct catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc cacctcgatc
    17401 ccacgattac gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg aacgtgcgga
    17461 tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc atcgttaata
    17521 ataatgattt ccagagcagt ccaggtttgc gtaattaaag attccataca agtgcggaaa
    17581 tcatcgcccg cattatataa cggaataata acactaagtt tattggtgct gttcatcata
    17641 aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt aagaaactat
    17701 taagcctgtg agatattatt tttacaggtg agagagtcac ttcagggtag agagataatg
    17761 agaacagcta tataaaagat cagcgcaatt acgaactctt tattacactg acaggatttt
    17821 acataattaa cagattatcc tcagataaaa gggctggaat accagccctt gttcgtgtta
    17881 atcccagctc agaataactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
    17941 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat
    18001 cagcgccgcc atcttgtacc aagtttcaaa catctcacga ccgtaaatgc ctttaatgaa
    18061 caagccttta aagatcactt tggtccagtc gatagacata tcagacggtg gaatacccag
    18121 catcgcaata cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc
    18181 gccggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc
    18241 attaaggttt tctttcgcaa cgttaaccgc acgggtgata cccattttgc gcgccagctc
    18301 aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac
    18361 cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag
    18421 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
    18481 gtcggagata ttgtcgggga ttttgaaagc gttgaacgcc ggaatcacca gatattcggc
    18541 gaagcagccc ggacggttaa caccgacacc aatggtgttg cggcacagat gggtacgacc
    18601 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
    18661 gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
    18721 cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
    18781 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
    18841 cggtacaggg acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
    18901 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
    18961 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
    19021 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
    19081 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
    19141 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
    19201 ccacagacgg tcacgcaact cgctacccgc ttcaaccatc tccagcactt tgatggaagc
    19261 ggcgacaatc gctggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
    19321 ccactcaacc acttctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
    19381 accgagcgtg ccggtgatga tgtcgacacg ccccatcaca tcgcagtatt catgggaacc
    19441 acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc
    19501 gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa
    19561 cacgccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
    19621 agcttcaagc tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg
    19681 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc
    19741 cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
    19801 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
    19861 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
    19921 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
    19981 gtggcttcca tcagccacgg tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
    20041 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
    20101 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatag
    20161 gtcgcgacga gatgatgcgt taacacttct gcaaaattca ggataaataa cgtgtatctt
    20221 gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg agcataatga
    20281 tgagtgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct
    20341 atctcagttg gcatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga
    20401 aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat
    20461 cgaaccattt tcctctcccc agatagttta ggttgaagag atcggaacgt ttgtaatagc
    20521 acatgacaaa aatgccctgg tcatcgtcca caatgttatt gtttagtgtt attttttgac
    20581 tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc
    20641 caataatata tacatgattg ccaatcatga aatcaaaagc ctgttgttga gatgtaacag
    20701 taagcccttt ttttatagtg aaaagatgca tcttattttc atcaaatggg aaatcccaaa
    20761 ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa
    20821 gaggcgtttt cactaaaccc atgttgatag ctttatttac aaaataggcc ttaaggttac
    20881 ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc
    20941 tatttgtaaa cgactcatct ttttgaattt tttcgatacg acttctgata tatctaaatt
    21001 tcttttttat atcgataacg ataaccgttg ttggttttcc gttgcgaatc gcttcaatcc
    21061 ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct
    21121 caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag
    21181 cagtccaatc ccctctacca atgtcaaaat atgcagtgat aattgtggtg gatgatttca
    21241 ttactaataa tgaccattaa tataaaaaaa gaattatatc ataaatatta acagaatgta
    21301 tactgtatat tatccattca aatgattccg ttatttatca tacatcggaa tattgatact
    21361 aaagcactat tcacatgcaa aaccaacatc cgccatgaag gactagctaa aacccaaact
    21421 agtttgttgc aattagcatc cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg
    21481 atggtatgat tacagacatt cgtgtctgag attgtctctg actccataat tcgaaggtta
    21541 cagttatgat catcgttacc ggcggcgcgg gctttatcgg cagcaacatc gttaaggccc
    21601 tgaatgataa aggcatcacc gatattctgg tggtggacaa cctgaaagac ggcaccaagt
    21661 ttgtgaacct ggtggatctg gatatcgcgg actatatgga taaggaagac ttcctgatcc
    21721 agattatggc tggcgaagag ttcggtgatg tcgaagcgat tttccacgaa ggcgcatgct
    21781 cttccaccac cgagtgggat ggcaagtata tgatggataa caactatcaa tactccaaag
    21841 agctgctgca ctactgtctg gagcgcgaaa tcccgttcct gtatgcctct tccgcagcca
    21901 cctacggcgg acgcacctcc gactttattg aatcccgcga gtacgaaaaa ccgttgaatg
    21961 tctacggtta ctcaaaattc ctgtttgatg aatatgttcg tcaaatcctg ccagaagcga
    22021 actcgcagat tgttggcttc cgctatttca acgtttatgg accgcgtgaa ggccataaag
    22081 gcagcatggc gagcgtcgct ttccatctca acacccagct taataacggt gaatcaccga
    22141 agctgttcga aggtagcgag aacttcaaac gtgacttcgt ctatgtgggc gacgtggcag
    22201 atgtaaacct gtggttcctg gaaaatggcg tttccggtat cttcaacctc ggcaccggtc
    22261 gtgcagaatc cttccaggca gtagcagatg ctacgctggc ttatcacaag aaaggccaga
    22321 tcgaatacat tccgttcccg gataagctga aaggccgcta ccaggcgttc actcaggcag
    22381 atctgacaaa tctgcgcgcg gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg
    22441 taacggaata catggcctgg ctgaatcgcg acgcataaga gctctgcatg aaaatactgg
    22501 tgatcggccc gtcttgggtt ggcgacatga tgatgtcgca aagtctctat cgcacgctcc
    22561 aggcgcgcta tccccaggcg ataatcgatg tgatggcacc ggcatggtgc cgtccattat
    22621 tatcgcggat gccggaagtt aacgaagcta tccctatgcc tctcggtcac ggagcgctgg
    22681 aaatcggcga acgccgcaaa ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct
    22741 acgtcttacc aaactccttc aaatctgcat tagtgccttt cttcgcgggt attcctcatc
    22801 gcaccggctg gcgcggcgag atgcgctacg gtttactcaa cgatgtacgc gtgctcgata
    22861 aagaagcctg gccgctaatg gtggaacgct atgtcgcgct ggcctatgac aaaggcatta
    22921 tgcgtaccgc acaagatctg ccgcagccat tgttatggcc gcagttgcag gtgagcgaag
    22981 gtgaaaaatc atatacctgt aatcaatttt cgctttcatc agaacgtccg atgattggct
    23041 tttgcccggg tgcggagttt ggtccggcaa aacgctggcc acactaccac tatgcggaac
    23101 tggcaaagca gctgattgat gaaggttatc aggtggttct gtttggctct gcgaaagatc
    23161 atgaagcggg caatgagatt cttgccgctt tgaataccga gcagcaggca tggtgtcgga
    23221 acctggcggg ggaaacacag cttgatcaag cggttatcct gattgcagcc tgtaaagcca
    23281 ttgtcactaa cgattctggc ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg
    23341 ccctgtatgg tccaagtagc ccggacttca caccgccgct atcccataaa gcgcgcgtga
    23401 tccgtctgat taccggctat cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc
    23461 agagcttgat cgacattact ccccagcgcg tactggaaga actcaacgcg ctattgttac
    23521 aagaggaagc ctgacggatg cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc
    23581 tccatacgtt gcccgcactc actgatgccc agcaggcaat cccagggatt aagtttgact
    23641 gggtggtgga agaagggttc gcacagattc cttcctggca cgctgccgtt gagcgagtta
    23701 ttcctgtggc aatacgtcgc tggcgtaaag cctggttctc ggcccccata aaagctgaac
    23761 gcaaagcgtt tcgtgaagcg ctacaagcag agaactatga cgcagttatc gacgctcagg
    23821 ggctggtaaa aagcgcggca ctggtgacac gtctggcgca tggcgtaaag catggattgg
    23881 actggcaaac cgctcgcgaa cctttagcca gcctgtttta caattgtaag catcatattg
    23941 caaaacagca gcacgccgta gaacgcaccc gcgaactgtt tgccaaaagt ttggactata
    24001 gcaaaccgca aacccagggc gattatgcta tcgcacagca ttttctgacg aacctgccta
    24061 cagatgctgg cgaatatgcc gtatttcttc atgcgacgac ccgtgatgat aaacactggc
    24121 cggaagaaca ctggcgagaa ttgattggtt tactggctga ttcaggaata cggattaaac
    24181 ttccgtgggg cgcgccgcat gaggaagaac gggcgaaacg actggcggaa ggatttgctt
    24241 atgttgaagt attgccgaag atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta
    24301 aatttgtagt gtcggtggat acggggttaa gccatttaac ggcggcactg gatagaccca
    24361 atatcacggt ttatggacca accgatccgg gattaattgg tgggtatggg aagaatcaag
    24421 tagagtgtcg ttctacaagt atgtctcttg cagatttgcc agctcaaacc gtttttcaaa
    24481 atttaaatct tgaaataata accaataagt tgacatcgga gataagatga cctcaacatt
    24541 atttttctct ctcgagaaaa aaaactggat agcgtactgg aacagagctc tcgtattctt
    24601 attcattacc acctattttt tgggtgggat tacaaggtat aaacatctta ttgttattct
    24661 tatgacaata acgacaatcg tctatctctg caaacggcca aaacactatc tctcactatt
    24721 taaaacattt ctttttggta gtgttgccat attaactatt gctgcattgc tgtcacttct
    24781 tcaatcccct gatgcaggtg ctagcatgaa ggaaattttc aaagctatta ttgagaatac
    24841 tttactatgc acaatagcaa taccggtcat attgagagac gagaaaagag aagatgtcga
    24901 aaaaatcgtt ttcttctcat ttattagtgc gttgggctta cgctgttttt ctgaattgat
    24961 tacctattat aaggactatc aacaagggat aatgccattc gcagattata gacaccgtag
    25021 catttctgac tcgatggtct ttttattccc tgcattgtta aatctctggc ttatcaaatc
    25081 agcaaaatac cgcatttctt ttgtggttct aagcgttatt tttatttttc tgatattagg
    25141 aactttatcc agaggggcct ggctttccgt gttagtcatt ggattaatat ggattctgat
    25201 gtttaaacaa tggaagttac tattagtagg agtaatggtt tccatcattg cattgtcggt
    25261 tattttcaca cataaggaga tgaccgcaaa gctaacgtat aaacttcaac aaactaatag
    25321 ttcttatcgc tatgcaaatg gtactcaagg cagcgcactc gatctaatat tagaaaatcc
    25381 tgttattggt tatggttacg gtaacgttgc atataaagat gtctataata aacgtgtcat
    25441 tgattatcca gaatggacct ttagacaatc aatagggcca cataattttg cgctattcat
    25501 ctggtttggc actggtttat tagggctggt aagtcttatg atgctatact gtgcaatatt
    25561 gaaagagtgt ataaaaaatg gcgtcaagaa taaatatcgc tcaccatata atgcatatta
    25621 tataatctta ctatctttta taggttattt tgttatccgt ggaaacgtag aacaaattga
    25681 accaaattta ttaggcgttt acgccggctt attattagcg atgaaaaaca agtaaaaaat
    25741 aaaaaaggct gcataatgcg gcctttttat ttattcaaac caattatgaa taacctcttc
    25801 gaatctttga gttacgccat cccaactgta gtgatcaaac acaaagtcct gaccttgctt
    25861 tgcaactgct gttaattcag gattcgctaa cgtttttaaa atatcgctgc ttatggagtc
    25921 tgcagtcata ggttctttta aatggaatcc cgtagtattt tctttgacaa attccgtcat
    25981 ccccccacgc gtactcacta aaacaggctt accagcaccc atagcttcaa ttgccaccat
    26041 gcaaaatggt tcctggaact gggaaggaat gacgactaaa tctgccagtg ggtaataaca
    26101 atgcatcttt tcaggcggta tgcttcctaa cataatgcaa cgatccttca aacgttttgc
    26161 aatttccctg acattgcgtt gatatgcacc tttgtcactt ttactcattt ctgtataatc
    26221 accaatcaca actaatttta aatttttgtg agcagcggcc aatttttcga atgattgcat
    26281 caacagcaat atgcctttgt cagggacgat acgcccagca taaaaaataa ttttctcttc
    26341 aggggagata ttaagttcgc ttctagtgat cggctgaaaa ttagattgat aggtttctaa
    26401 atcaattcca tttggtacaa tctcaatatc tgcatttgct aaataagatt ggtaatactt
    26461 tttcaagtat aaactaggga ctatcatttt aacattttgc tcaagcaatt ttggttcaaa
    26521 tgcattatgc atatgtataa ccactcttgc ctgaggagct cgcttacgaa tttgagtgta
    26581 tagcttcatg ctattatgaa ctacaataac actttctttc gttataggaa aatcatgtgc
    26641 aatattcaat attctctgtg aatatggaag aggatcaagt cttgtccatt tttgaaataa
    26701 ccgcttatat atgcgactga atccaaccct atgaatacta caattatcat taacaaaggt
    26761 gtaatttgag tatcctggat ttttgataca aacaatacga tttggaaaat tggtacgctg
    26821 agcaacctga tacatccacg tttcaacagc agccgctccg cgaggaggaa ttgaaaaaat
    26881 aggagtaacc gtaaatatta ttttatcaac catctcgtat attaaccttt cataacatta
    26941 tatttaattg ctgtaaaaac accatcgaga aattttttct ggtaaagcaa gtgtttatat
    27001 ttttctttgt gctcatgttt tttaacagct tttttataag gtatatttct ccatggtgat
    27061 atattataaa tattacgaaa ataatctgca gaggcgtagt tcgcccaatc atgccatggc
    27121 ttagttatac cagtataatg tatgaagaca gtgtcatcat taataaaccg ggtgtaatat
    27181 tcactatttt tttcttcaaa ttctgacttt atagtataaa tacaattata ttttctcggt
    27241 aatattttag catgatgtag aaacattaag tttaaaatat cttgatccgg gtactttaat
    27301 ttttttataa ttgactcatc tgataataga tcaaagaatt tttccgttag tctttgtttt
    27361 aaccattccc gtaaattaat atacattaca ccagagttaa aatagctacc gttaaaatcc
    27421 tcattacaca aacgctcagc gcttttactt tgcatagcat ctacatcaat taccactgcc
    27481 ccatattcat ctttaaactc taatgctatt aactcgttca atgaaccctt acagacgata
    27541 tcagcatcaa gatataataa tgaatccaat cgcgcagaaa aataatcaaa tgaaagcaaa
    27601 cgataataaa tagatactgg ccagatattt gattgaggta gcgccttcaa tggctcagaa
    27661 tcaatatgat ataatttaat tactgtttga tacttcgttg ctaattgtga taataattca
    27721 atatactctt tgtctaagta atcagcgatt atgtgaaagg taaatttgtt ttgtttattc
    27781 ttttcaagaa ttgaagcaat tgaaatcgca gccccaacct ggtagttctc atcaataccc
    27841 catgagacat taaacgtttc ccgttcatca agtttaacgg ggcgcttgtc cagttcaatt
    27901 atatctttaa attgagtaag atgtttaaaa tccaatttaa ttacccttca tcataatcat
    27961 tatttgctct tcaatcctag tttgactttt acatccctga ttactttttt aattttcttt
    28021 ttgaatataa gatatgtata gccaaagtct ttcaattcat tttttatatt atagtgccgt
    28081 tcaagatcaa tgcgatcttt tgcttttaaa acagcggttg tttttttacc tgaaaggtca
    28141 attagtctta aacctttttc ggatactata aaatttcctt tatgaggatc gccagacacc
    28201 attccatgct gatgcaactc ttttatcgat tctgataatt gatccttcag atcttcagaa
    28261 atttccaaat actcatttaa cccgacacct tcaatatact caatgagcat aatataataa
    28321 tgagcgaaat ttaatgtttt acgttcagct agcaaaaaat agtcattaat tgattggatg
    28381 ccttcaccac gtacccgatc ggtttgataa attaaatttt cataataatc tttttttatg
    28441 caagatttta agaaacgttc ggtcatttta tgctttggcg cataaacttt aagtacaaga
    28501 ggtccacgag cagtgtcaat aagaatgact ttagtatcat caatagaacg aaaaactttt
    28561 aatgttttaa ccctgcaagt taaaaagtca tttagaactt gctcataaaa gggatcatta
    28621 tcttttatga aaactttcaa cccatttatc gttttattat atatcataat gaattatttt
    28681 aaccttaaat ctttagaagc atttttcttt ataatacttt aaataattaa aaatgcctgc
    28741 catataatgc ttttgtttaa atttatgttt tgcacaatac cgatactgat tgctatttac
    28801 aggtttaagt aaatcttctt tactccacgg agaagctgct ttggcgatca aaaaactacg
    28861 tgaaaccgga taatcagccc actcatgcca gggttttgta ggtcccacat agtgtataaa
    28921 cacggtgtca tcattgactg gattatcaac tttgtctttt aattcatagt taatactata
    28981 tcgggtatta tatttctccg aaataaactt cactttacca ttcaataata cattcagtac
    29041 atcttgatca aggtgggtga ttttacttac ccaatctggg tcacgcaaca tttcaatagc
    29101 ttttgacgaa atgttattta gattccactc atcaatattt atcagtaaaa aaccagcatt
    29161 gaaatatcca gaagctaact gtggtgtagt taataccgag gctcgatttt gccaccattc
    29221 aatatctctt tcagctacaa ctgcggcaat ttcattagta gaaaattgat aatctaagag
    29281 ttctttaata ctacccttgc aagcaatatc tgcatcaaga tatagtattt tttcatgttt
    29341 atgatagaaa taatctgcaa ttataaatcg aaaatatgtt gcgtaagtcc agtttttcgt
    29401 gcttggtaat gacttcagct tatcacaatt gataacataa atattaattc ttgaattata
    29461 ttgttttgct aaatcagaaa aatataattt gtctttatca ctgatataat ctgtgaaaac
    29521 atgaaattca caagagattt ctctattgtt taatagaata gatgcgatgg ctacaccaca
    29581 tccaaaaaga aaatttctgt caataccaaa tgctatatca agagtatttt tagatcttat
    29641 attttgataa tgaaaattat actcaaaaag gatgatgtca ctatcattga gttgagacat
    29701 atttattctg tatccaaaat tttggcaaaa ataagttacg ggttacaacg attttcgaat
    29761 cgttctttcc ctgatttttg tggcttttgc ttctgcttgc gacagcagtc cttgttcctg
    29821 cttgagaatg tctttaagcg gggcggcaaa atacaccttc ataaagcgcc agatatcccg
    29881 ctgagtcagg ccaatattca ttgaagaaaa ataaagccct ataagatctt tatcgcgcca
    29941 acgacctgga acgcgcgtgc gaagctgcgc ccggtgtagg tcaattaccg aaatttttaa
    30001 ctcctcttcc ttaccggaaa aaggcaagtg cagcaggaaa tgacagatat aacagtcacg
    30061 atggttaatg cccgcagcat gcatatcgcg caccatcgtc gcgacacgct taataagcat
    30121 acgctttacg cgaacatctg gtgggttggt cgcccagtca gcacagtaat cttccagact
    30181 gatggttggt gtcagatctt cggtaataat aaacgaggtt ctggtcagcg gattcatgcc
    30241 tttttcgccg aatgccaccc catacatagt atcaacgccg acatcccgca gtcgatgaat
    30301 cgcattccat tcgcggtctg cgcctaatac tggcatccgc aatgagagta aatttttgat
    30361 tatctctttc agggtcgtgc cgcgatgcca tttgagaaaa tagctttttc ccgccatttc
    30421 aaagcgcaga gtacggcgag tttccagttc acgaaatacc tcaccctgca aggttttaac
    30481 ttcctcaaaa ggatctttgc cgcgccataa cgtggcaaac ggctctttaa gttcaaccat
    30541 ccagaccacc cgttatgata tccgccgctt tctctggcag actgtataaa tcttgtgtat
    30601 cagcataatg tcgcgcattt tccgcccaag cctggcgcaa tgaagattgc gttaacgctt
    30661 tgcgtaaaat ctcattcaat gtttcctggc ggaatggctc agcaatagcc tcgccgcaat
    30721 tagcgtcgac aatataatgc gcatagccac aaacggcagt tgttagtacc ggtaatcctg
    30781 cagtaatcgc ttccagcagc acaattcccg ccgcttcctg gtaggcagga tgcagtaata
    30841 aatccgccgc cgccattaat tccgagacat cgttgcgccc cgagaagaag tgaacattac
    30901 tgcgcacgcc gcgtttttct gccagtgcct caaattttcg cggtttatcc tgcccaacaa
    30961 catatagcaa tgtgttgtgg cgcagcgaat ccggtaacga agcaagtgct tcaatggaac
    31021 gatcgacacc tttacgcgtg aagtctgaac cgacctgcaa caataaatat tgttgttcgg
    31081 ttattccatt cttcttacgg aagatttcac ggctattggc tggctgctgg ctatatttac
    31141 gatcaggata aatccccggt ggcagaatat gaaaacgctc cgcttcagtc tgataatgtt
    31201 tctggaaatc ggcgatttgc ttatctgtca gcatcagcag ctgtgtcggc ttgccctgtt
    31261 cgaaggttgc ccgctcaaag gcggcataat ggcgataacg tgacgtcagg cgatagaaaa
    31321 agcctttttc ctgcgctact ttctcggcat aacaaacatc agcggcataa taaacgtcca
    31381 gccccggcat tttgttgaat ccaacgactt tatcgacggg atgttcgcgt aaatgttttt
    31441 gcacccaggc aaaatactcc gcattgcgcc cgtgattggt atgcgattta accggcactt
    31501 tgatcagttc aaatacatca gggcattcgc cttcccacga ctgggtataa acccgaacat
    31561 gatgacctcg ggcggcgact gtctgagcaa tacgcataaa atcgcgctgc aaaccgccaa
    31621 agggaaaata tttatataaa caaaaagcaa cgatcataac gacgtacctg tcgtggagga
    31681 gggcagtaat ttatcgacag cagcaatgac atctgccgcc ggaataaccg aaagatacat
    31741 ctcatttcgg tcacgctgat cgcgcgttgg catttcccgg taatctcccg cccagaattg
    31801 aatcatgtta tttgaccagg gacgccagaa aatatgatct gttgcgccaa acagcgatat
    31861 cagcggcgta ttaactgcag cggcaatatg cgccggtgcg gaatcaacgc caataaacag
    31921 ctgcgcatga tcgattaacg caccaagttc cgggaaggtc acctttccag ccagcgccgt
    31981 tactggtggc gtctggcatc cctgcgcaat ttcattgacg caggccagat cgtctttatc
    32041 tgggccggac gtcagaacga cttcataacc acgagcatgt aaggcatcaa tcacagcgga
    32101 aaacttggcg ttgtcccagc atttgaagat ttgccgcgcc gtaggttgga taaccacata
    32161 attttgtcca acaccagcgt gatcaagttc gcgacgcata cgtttccagc ttgcaggcgg
    32221 gtaactcatg gttgtctgct tcaccaacga atcaagtccc aatggtgtca gcacggataa
    32281 gttactttcc accacatttc caccctgcaa cggcaccaaa tgggtgaaac ttttacgcca
    32341 aaaagcagac tgccgatgat gataatcctg ggaaattttc acacgagcat ttaataagcg
    32401 aaccagtata gcaaccatcc attgatcggt aagattgacg ataaggtcat acttattggc
    32461 acgtaatacc ttgatgagat gaaaaaagtt ggcaattttt tctgaggctt ttgctttttt
    32521 attttttatg ccgtagagcg cgttaatctc tggattttca gacaggatcg ggatggtgtc
    32581 ctgataaagc agcacatcga tttttgcgtc agggtaattt tttttcagag aactaatgac
    32641 gggagtagtt aataacatat ccccatgaaa acgcatctta atgagcaaaa ttcttcgaaa
    32701 tggcttatcc acaagtaact ctttttgttg agattatccg attaaggtga agcagaaaaa
    32761 gcacgctacc gccccaggct caacagctac ctgaatactg ataccgcact gatattgacg
    32821 cgctagtgta tcacttcttt cgcggcaatc cgacccgaat gatttttaag cacattgttg
    32881 attttcaata aagaaatcaa tcaaataaaa tcattagcaa ccatattctt tcactgttgc
    32941 tctgcgattc cttcctgcac gtacagcaga aatcaatgaa tcgcataaaa atacaaaaaa
    33001 agtatagatg ttcagcaaga attggcgggt aaattgctat ttaa
//
LOCUS       DADWTH010000039        32327 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.039, whole genome shotgun sequence.
ACCESSION   DADWTH010000039 DADWTH010000000
VERSION     DADWTH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.039"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNY02_001923"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNY02_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBB9433473.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JNY02_001924"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JNY02_001924"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBB9433474.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JNY02_001925"
     CDS             complement(3736..4092)
                     /locus_tag="JNY02_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBB9433475.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JNY02_001926"
     CDS             complement(4096..4512)
                     /locus_tag="JNY02_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBB9433476.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRSVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="JNY02_001927"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="JNY02_001927"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBB9433477.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5960
                     /locus_tag="JNY02_001928"
                     /pseudo
     CDS             5738..5960
                     /locus_tag="JNY02_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JNY02_001929"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JNY02_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBB9433478.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JNY02_001930"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JNY02_001930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBB9433479.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="JNY02_001931"
     CDS             complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="JNY02_001931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBB9433480.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="JNY02_001932"
     CDS             10540..11523
                     /locus_tag="JNY02_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBB9433481.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JNY02_001933"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JNY02_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBB9433482.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JNY02_001934"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JNY02_001934"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBB9433483.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14449)
                     /locus_tag="JNY02_001935"
                     /pseudo
     CDS             complement(13465..14449)
                     /locus_tag="JNY02_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14767..15282
                     /gene="zur"
                     /locus_tag="JNY02_001936"
     CDS             14767..15282
                     /gene="zur"
                     /locus_tag="JNY02_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBB9433484.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15324..15533)
                     /locus_tag="JNY02_001937"
     CDS             complement(15324..15533)
                     /locus_tag="JNY02_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBB9433485.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15649..16974)
                     /gene="dinF"
                     /locus_tag="JNY02_001938"
     CDS             complement(15649..16974)
                     /gene="dinF"
                     /locus_tag="JNY02_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBB9433486.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="JNY02_001939"
     CDS             complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="JNY02_001939"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBB9433487.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17765..18133)
                     /gene="dgkA"
                     /locus_tag="JNY02_001940"
     CDS             complement(17765..18133)
                     /gene="dgkA"
                     /locus_tag="JNY02_001940"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBB9433488.1"
                     /translation="MVNNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18304..20727
                     /gene="plsB"
                     /locus_tag="JNY02_001941"
     CDS             18304..20727
                     /gene="plsB"
                     /locus_tag="JNY02_001941"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBB9433489.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="JNY02_001942"
     CDS             complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="JNY02_001942"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBB9433490.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVASALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="JNY02_001943"
     CDS             complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="JNY02_001943"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBB9433491.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22487..24067)
                     /locus_tag="JNY02_001944"
                     /pseudo
     CDS             complement(22487..24067)
                     /locus_tag="JNY02_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24296..25216)
                     /gene="malM"
                     /locus_tag="JNY02_001945"
     CDS             complement(24296..25216)
                     /gene="malM"
                     /locus_tag="JNY02_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBB9433492.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKIQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="JNY02_001946"
     CDS             complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="JNY02_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBB9433493.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26780..27895)
                     /gene="malK"
                     /locus_tag="JNY02_001947"
     CDS             complement(26780..27895)
                     /gene="malK"
                     /locus_tag="JNY02_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBB9433494.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28260..29450
                     /gene="malE"
                     /locus_tag="JNY02_001948"
     CDS             28260..29450
                     /gene="malE"
                     /locus_tag="JNY02_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBB9433495.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29604..31147
                     /gene="malF"
                     /locus_tag="JNY02_001949"
                     /pseudo
     CDS             29604..31147
                     /gene="malF"
                     /locus_tag="JNY02_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            31162..32052
                     /gene="malG"
                     /locus_tag="JNY02_001950"
     CDS             31162..32052
                     /gene="malG"
                     /locus_tag="JNY02_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBB9433496.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8228 a         8289 c         7802 g         8008 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtgaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acgctgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aataatgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt ttacgtgcat
    14221 gccataaatt atgcgtgatt cgttgaataa ataaatctat ttttgcaata attatatccc
    14281 gatgacgcag cgctgcataa tcaatagatt taccatatat attatttaat ttacctttga
    14341 ttgaacctct attatccaat ttatctttgg ttgcatcttt attttcgaat tgatttgaat
    14401 tgagtgtttt attaaagtat ccagttgcac cgggtattat cttcaacatg attcatctac
    14461 cttttttgtc gtaccgtgtc ctccttataa ttctttttcc atctggtcac tatcaaaaat
    14521 cgctcataat ttctgaacta caggtatgcg tttaaaccca gttaaataaa tagcgagtta
    14581 acttgaagca atttatttaa aaatgaatta ttaattatta tcgtgttgat aatggtacga
    14641 caaggcaaca caacacctta cctacaaaac agaaaatcaa cattacggca acaataaggg
    14701 ttctcggtgt ttttcctgga acatggtaaa gtaaggacat tcttaacccc cactttgagg
    14761 tgcccgatgg aaaagaccac aacgcaggag ttattagcgc aggctgaaaa aatctgcgcg
    14821 cagcgtaatg tgcgcctgac cccacagcgc ctggaagtgt tgcgcctgat gagtctgcag
    14881 gatggcgcta tcagcgctta tgatctgctt gatttactgc gcgaagctga accgcaagcc
    14941 aagccgccaa cggtttatcg cgcgctggat tttctgcttg agcaaggttt tgtgcataag
    15001 gtggaatcca ccaacagtta tgtgctctgt catctgttcg accagcccac gcatacgtca
    15061 gccatgttta tttgcgatcg ctgcggcgcg gtgaaagaag agtgtgcaga aggcgtggaa
    15121 gacattatgc atacgctggc ggcaaaaatg gggtttgccc tgcggcataa tgtgattgaa
    15181 gcacatgggc tctgtgcggc atgcgtggaa gtggaagcgt gtcgtcatcc tgaacagtgc
    15241 cagcatgatc actctgtgca ggtgaaaaag aaaccgcgtt aagagggcgt acatccttgt
    15301 acacgtcggg caggagggat taattaccag cgatattcat tgcgggtttc ccaatccacg
    15361 acctcttttt ctgcctgatc tttctgataa ccataacgtt cctggatttt accgaccagt
    15421 tgatcacgtt taccttcaat gatcgtcata tcatcatcgg tcagtttgcc ccattgctct
    15481 ttcactttac ctttaaactg tttccagtta ccgccggctt catctttatt cataatcaag
    15541 acctcatcgt taggttgtga atgagagtac gttcactttt cttctgaacg tgagattaag
    15601 tgtagtcagc aatttggctt aggataattt attcagaatt tttaaccgtc acgttgcggc
    15661 aaaccaggta ccgttgcgcc agtgacgccg ccagatagcc gccagagaaa gcccgcgcaa
    15721 cgccagaaag acggttaatg ccagccacaa accatgatta cccagccacg gcagcgtaag
    15781 gagcgtcagc gcaaaacctg cggcggccac cgccatactg ttacgcattt cggcggcacg
    15841 cgttgcgcct ataaacatgc cgtccagcag ataacaccag acgccaacca acggcaaaat
    15901 cacctgccag ataagatagc ggtcagccag ctgctgaatc tgggttaacg acgtcagcaa
    15961 cgcaatgatg tgttcccctg ccagcaaata aaccaccgaa aacagtaacg ctacaatccc
    16021 cgactggcgg cacgctgccc gccatacatc cagcaactgg ctaccgtcgc gcgcaccgta
    16081 tgcctgaccg gagtgcgctt caaccgcgta ggcaaaacca tcaagcgcat aggcggtaaa
    16141 ggtgagtagc gtcatcagaa ccgcgttaac agcgataatg tcactcccca gtcgcgcacc
    16201 aagtacggtg atcgcgccga aacagagttg caacaacagc gaacgcagca tgatatcgcg
    16261 gttaagcgcc agcaagcgac ggaagtttcc tcgccaggca gttttcagca tttcgccgga
    16321 aattccgcgt agtttgagga ttttacgcac cattagcaga ccaatcagca atgttgcata
    16381 ttccgcaata accgtcgcca gcgccgcgcc ctgcacgttc atatgcagcc ccatcaccag
    16441 ccagacatcc agcacaatgt tgaggatatt gccgaccact aacaaaatta ctggcgcacg
    16501 ggcatattgc acgccgagta accaaccgag taataccaga ttcgccagcg acgccggagc
    16561 gcttaaccag cggatttcaa gaaagcgccg cgcctgttcc aggaccgctt cactaccgcc
    16621 aacaatatgc agcgccagat cgataatcgg cgtacgcaac agcgcaatta acgccccggc
    16681 ccccaacgcc aacagcaacg gttgcaccag cgcacgggct aatgcctgag gatttttggc
    16741 accataagcc tgcgcagtca gcccggtggt gctcatgcgt aaaaacagca acagcataaa
    16801 gagaaagctg gtcgccgttg caccaaccgc cacgccgccc aaataaaccg gactatcaag
    16861 atgaccaatt accgccgtat cgaccagtcc cagcaacgga acggtgatat tggagaaaat
    16921 catgggtaag gcgagatgcc agagtgcttt atcagatgaa gtgaggaatg ccatgcagac
    16981 aagcctgatg aagagagatg aaaaacaaac cgcgacgcca ggcggcatcg cggtctcaga
    17041 gatatgttac agccagtcgc cgttgcgaat aaccccaacc gccagccctt caatggtgaa
    17101 gctctgctga cgaaggtcaa cgacaattgg tttaaactcg ctattttctg gcaacagttc
    17161 gactttattg ccctgttttt tcaggcgctt aacggtaact tcgtcatcaa tacgtgcgac
    17221 aacgacctga ccgttacgta catcctgagt tttatgcact gccagcaagt cgccatccat
    17281 aatgccgata tctttcatcg acatcccgct gacgcgcagc aggaaatcag cattcggctt
    17341 gaacaaggaa ggatcgacct gataatgacc ttcaatatgc tgttgcgcca gaagcggttc
    17401 accggcagcc acacgaccta ccagcggcaa cccttcttcc tcttcctgca acagacgaat
    17461 cccgcgtgat gcgccggaaa caatttcaat aacgcctttg cgtgccagcg ccttcagatg
    17521 ttcttcagcc gcgtttgggg aacggaaccc caaacgctgc gcgatttccg cacgcgtcgg
    17581 cggcatacct gtctggctga tgtgatcacg gatgagatca aacacctctt gttgcctggc
    17641 cgttaacgct ttcattccgc cccctgggtg tatatacagt tatactgtga gtatatacag
    17701 caaaaggcga ttttggaacc ataaactgca caataaacca gagatttatc gaaatctgga
    17761 agggttatcc aaaatgcgac cataacagga tgcaccaggt aatcacggcg acgataatgg
    17821 caatcagcac cgcggcggat cccatatctt ttgcgcgtcc ggaaagctca tggtattcag
    17881 agccaattcg gtcaaccact gcttcgatgg cgctattgag gatttccaca atcatcacca
    17941 gcatcacgga gctgataagc agcacgcggg taattgcgtc cacatccagc cagcaggcga
    18001 tgaccaccgc caacaatacc gctacgcctt cctgacggaa tgccgcttcg ttgatccatg
    18061 cagcgcgtaa acctttccag gaatagccag cagctttgat aattcgggtg aatccagtgg
    18121 tattattgac cattgaaaga acctttttac attatgagcg tcaatatcag tgtaccgtta
    18181 aatccgatga ttcgggtttg cgtaacgccg cgcgaaacat gagcggatac cacagaattt
    18241 cccatgactt tctgctatcc ttgccgcgca tttgcattat taaccagagg ctttacatcg
    18301 tttatgtccg gctggccacg aatttactac aaattactga atttaccatt aagcatcctg
    18361 gtaaaaagca agtctattcc ggcagatcct gccccggaac tggggctgga tacctctcgt
    18421 ccaattatgt acgttttacc gtacaactcg aaagcagatt tgctgacgtt gcgcgcccag
    18481 tgtctggcac atgatttgcc tgacccgtta gagccgctgg aaatcgacgg cacgctactg
    18541 ccgcgctatg tgttcattca cggcgggccg cgtgtattca cctattacac gccgaaagaa
    18601 gagtctatta agcttttcca cgactatctc gatttgcacc gtagcaaccc aaatctggat
    18661 gtgcagatgg tgccagtatc ggtgatgttt ggtcgcgcgc cggggcgtga aaaaggcgaa
    18721 gtgaacccgc cgctgcgtat gcttaacggc gtacagaaat ttttcgctgt actgtggctc
    18781 ggtcgcgaca gttttgtgcg tttctcgccg tcagtgtcgc tgcgccgtat ggcggatgaa
    18841 cacggcacgg ataaaactat cgctcagaaa ctggcgcgcg tggcgcgtat gcactttgcc
    18901 cgtcaacgtc tggctgccgt aggcccacgt ctgcctgctc gtcaggatct gtttaataag
    18961 ctgctcgcct cccgcgccat tgccaaagcg gtagaagatg aagcgcgcag caaaaaaatc
    19021 tcccatgaaa aagcgcagca gaacgcgatt gcgctgatgg aagagattgc ggcgaatttc
    19081 tcttacgaga tgatccgcct gactgaccgc attctgggct tcacctggaa ccgattttat
    19141 cagggcatca acgtccataa cgccgagcgc gttcgccagc tggcccacga cggtcatgag
    19201 ctggtgtatg tgccttgcca ccgcagtcac atggactacc tgctactttc ttacgtgctg
    19261 taccaccagg ggctggtgcc gccgcatatc gccgccggga tcaacctgaa cttctggccg
    19321 gccgggccga ttttccgccg tctgggggcg ttctttattc gccgtacttt taaaggcaat
    19381 aaactttatt ccaccgtttt ccgtgagtat ctcggcgaac tgttcagccg tggttattcc
    19441 gtcgagtact tcgtggaagg cggtcgttcc cgcacggggc gtttgctgga tccgaaaacc
    19501 ggtacgctgt cgatgaccat tcaggcgatg ctgcgtggcg gcacgcgtcc gattacgctg
    19561 attccgatct atatcggtta tgagcacgtc atggaagtgg gtacttacgc caaagaactg
    19621 cgcggcgcga cgaaagagaa agagagcctg ccgcagatgc tgcgcggttt aagcaagctg
    19681 cgtaatctcg gtcagggtta cgtcaacttc ggtgaaccaa tgccgttgat gacctacctt
    19741 aaccagcacg taccagactg gcgtgaatct atcgatccca tcgaagcggt gcgtccggcc
    19801 tggttaacgc cgacggtcaa taatattgct gccgatctga tggtacgcat taacaacgca
    19861 ggcgcggcaa acgccatgaa cctgtgctgt actgcgctac tggcatcacg tcagcgctca
    19921 ctcacccgcg agcagttaac cgagcaactc aactgctacc tggatctgat gcgcaacgta
    19981 ccttactcca cggactctac cgttccttca gccagcgcca gcgagcttat cgatcacgcg
    20041 ctgcaaatga acaagtttga agtcgagaaa gacactatcg gcgacatcat cattctgcca
    20101 cgcgagcaag cggtgctgat gacctactat cgcaacaaca ttgcgcatat gttggtgctg
    20161 ccttcgctga tggcggcaat tgtcacccag catcgccaca tctcccgcga cgtattgatg
    20221 gagcacgtca atgtgcttta cccaatgctg aaagcggagc tgttcctgcg ctgggatcgc
    20281 gacgagttgc cggacgttat tgatgcgctg gcaaatgaga tgcaacgtca ggggctgatt
    20341 accctgcaag atgatgagtt gcatatcaac ccggcgcatt ctcgcaccct acagctgctg
    20401 gccgcaggcg cgcgcgaaac gctgcaacgt tatgccatca ccttctggtt gttgagtgcc
    20461 aacccgtcga tcaaccgcgg tacgctggag aaagagagcc gcaccgttgc gcaacgtctc
    20521 tccgtgctgc acggcatcaa cgcgccggag ttcttcgaca aggcggtgtt cagttctctg
    20581 gtgctgacgc tgcgtgatga agggtatatc agcgatagcg gcgatgccga accagcagaa
    20641 acgatgaagg tttatcagtt gctggcggag ttgattacat cagacgtgcg tttgacgatt
    20701 gagagtgcga cgcagggcga agggtaatca gagagaattg ccggatgcgg cgaaaacgcc
    20761 ttatccggcc taccatgacc tgcaaattca ataaattgcg attcaccggg taggcctgag
    20821 aagcgcagcg catcaggcaa cccagaagaa agccggatga tcatccggct tttttacatc
    20881 atcagaaatg ccagtaactc attgccagtc ctaaaaacag taccagacca acatagttat
    20941 tattcataaa tgctttaaag caggcttcac gctcgcggtt ggcaatcagt ttttgttgat
    21001 aaacaaacag cgcgctagcc accacaattg accagtaata tccccagcct aagccattta
    21061 actcaccgat gatcgccatc agtgccagta cgccaatctg caaaatacca ataatcaatt
    21121 tatcgtattg gccgaacagg attgccgtgg atttaatgcc aatcttcaca tcatcatcgc
    21181 ggtcaaccat cgcatactgc gtgtcgtaag ccaccgccca gagaatattg gcgaggaaca
    21241 ttaaccagca actcaatggc accgactcac tcacagcggc aaaagccatt ggaatcgacc
    21301 agccaaacgc cgcgcccagc accacttgcg ggagatgggt ataccgcttc ataaacgggt
    21361 acacccacgc caatgccaga gcggcaatcg acaacagaat ggtcatcgta ttcagcgtca
    21421 gaaccagtaa aaacgaaatc agtaccagca ctacaaacag cgcgcgcgcc tctttctctg
    21481 ttaccgcgcc gctgggaagt ggtcggttcg ccgtgcgctt aacatgacca tcaaacttgc
    21541 gatcagcata atcattcacc acgcaaccag cagcgcgcat cagccagaca cccgcgacaa
    21601 acaccgccag gatccagagc tgaggaacgc ccggtgtcgc cacccacaac gcccataatg
    21661 ttggccagag cagcagtaac gcgccaattg gcttatccgt acgcattaag cgatgaaacg
    21721 ccagcagctt attctgcgtc agactccact ccatattttt ttcctcttag tacaacggtg
    21781 acgccggtaa aaacagttct gttagcaaca gcggtttacc gcttaatcgc aggcgggaac
    21841 gtcgccccca cagtccggca tcacggccta tctcgataaa atcccgggtt agtgtcgatg
    21901 atgtgaacag atagcgtcct aacggcgttt tacccaattt ttgtaacgct agttccggcc
    21961 cgcttaatgt tgatacagga acgacggtac gaccggcaag ccacggttca ccatcggcac
    22021 ataacaaaat ttcacgtaac cagtaacgag actctttcgg cagtagcggc agttcttcgg
    22081 ggatttcatt ctgctcgaca aacccctcgc ggatcatcgt cacactcacc gtttttccct
    22141 gctgttcaaa acgttttgtc atggaatcct ccagcaacag ccagtcgaac agttgcggat
    22201 ccagggcagg gatctcttta aaatagcgca gcgcacgcag ttgcgttaac gcggggtgtg
    22261 acatgccgaa ctctccgtta cataacgtaa aggcattgta tcgcagaaaa gtgtttacgg
    22321 gagatgtaaa gcaaattaag tgatctaatg cgcaacaaaa acgcaacaat cgcatgaagt
    22381 ggtaaaacgc tgttgaacct tacgctttgc cgatgcccgt gctggcgagg gcaaccatag
    22441 gggcatcacc gcccatgatg ctaggaacgg tgatgttttc ctttacttaa atacctttat
    22501 tctttatacg agtagctcgc caactcgcag taaaaattgg gaaaagcgca tatttaccat
    22561 gagatttgga gctgaaaagt gtttcctgag taagattaat aggcaacatc ttatgtctgg
    22621 atttatagat acacgtggaa ttccaattgg tatcttttgt aggattcaac atatcatgta
    22681 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
    22741 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
    22801 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
    22861 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
    22921 tatttttatc aaccagccct ttattcatat taaatctgtt aataaaatgg atcatctgtt
    22981 gggtatcgaa attcacctta ctgaatcgat gttgacgcat aaaattatcg aacttgtcat
    23041 cttccccaga atgtatataa ctacgtataa ttggatcttt tatataagga caatggctaa
    23101 atgttttctt catattctca ataaagtttt tattaacaat attgagaatt tttaattgca
    23161 tcaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
    23221 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
    23281 aatcaatagt taattccaat tgttctgtaa ttcaatctta aaaattccct gtcgacattc
    23341 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
    23401 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
    23461 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
    23521 tatcaagaat attttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
    23581 tgtgtaaacg tcgacattta aatatgacat tatgaactgt tgccttagta aaattgcaat
    23641 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
    23701 tagaaataac gtcaccttta aaatgacaat ttgttaaatc aaaatcagac aggtcgatat
    23761 acccctgcgc atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
    23821 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
    23881 gatttttttc gttccgttgt tttatattta aaaatatatc actgatataa atgaagttat
    23941 ctctgtttaa acctgtcacc ccattgaaat tacgtagtaa atcaagatct actatattat
    24001 ttccttgcac cacatcatgt ttcatcaaag agttattgcc gccagaattt gtgatatgac
    24061 ctatcataaa acctctgtaa tacagaaata aaataacctg gaaaagtagc ctgagcgtcc
    24121 agacactacc ggcgaaagga aaagaatctc tgataatgca ttgaaataat ggacattctt
    24181 agtgaagaag aagtgaagta ctataaaaaa tcgctcatat cccaggaagg aagcataaaa
    24241 aaaggtgcgc caggagacgc accagttgtt gcaaaatcag cactgtgggg cgtaattacc
    24301 ccttgccttt tacactgctg ataaaggtgg aacgggcaga tgtcgatccc aggcgttcag
    24361 cttcatcaag cagttttaac gccttatcaa catcaccttt cgcgacagcg tttttgatcg
    24421 cggtattaaa ataactttcc gtgtcgttga gcatcggctc gcttttcttc accggcaccg
    24481 gagcaggtgc agccacagct ggtgccgccg tgttgcctac cgtaaccgga gctggagcgg
    24541 aagaaccaaa tagtggtcct accaacacgc tggagctgga gttcgttttg actttcagtt
    24601 tcagtaagcc atcgctggta tgacgagcaa ccggatcggg aatatccggg atcgagttac
    24661 cgacgccttt ggcataggct ttagccgggt cgagcagttg ggtcgtctgc tggagatctt
    24721 tttccatggt aaagaccaga acataaagtt tttgctgccc caacgccggt gtcaggcgca
    24781 taacgccttc cagccgatct gcactcatca cgcctagttc ctggtaggtg aaataactgc
    24841 tggggaagaa ggctgatggg gtcatgttct gatcaataat cagcacgttc ggcgcaaaaa
    24901 cgctggtttg tttgttcact tcgctggtca gcgtcagggt cagttcgcca atgtttgccg
    24961 ggacgctgta cgcagcaacc ggaccactga tgccgggaac gttcagttgt tggccgccgg
    25021 tcgccagttg ggtggtctgg attttagatt gatcgaccgg tgtccaggtg agttgttgca
    25081 gcgcggcaga tggaatggct ggcgcgtcgc tggtgttttg cggtacgtag ttaacatcgg
    25141 caaggctaat tccaggcgcg cttgccagta accctgctga taaacagagg gcgatgagac
    25201 ttttattcat tttcattgtt ttcacctcaa aatctggagc tcagcggtag ccaggcaata
    25261 gcgcgctaaa cccgataatc agaggggctt tcgccccttt cagataatga caacctgttt
    25321 ttatgccgga tgcggcgtaa acgccttatc cggcccaggt tttgctatta ccaccagatt
    25381 tccatctggg caccgaaggt ccactcgtcg ctgtcgccac gaccgaagct gccgccgttg
    25441 aaatcagcag gaacggcttt gccgtagttc gggttaacct tgctatcgcc gttgtagtcg
    25501 taaccccatt tctcatccca cttggcgtag gttgcgaaga cacgaatagc cgggcgtgac
    25561 cagatgctgt cgccagcctg ccattgttgt gcgagggtaa ttttgtactg attgttcttg
    25621 tcgccggtgc gctgggattc gacgttgtcg tagccgattt ccatcacggt gctcatgatt
    25681 ggcgtccact tgtacatcgg gcgaatacca acggtccacc acttggtgcc gttgtcgtta
    25741 tcccagttga tatcctggta catacccacg tacatcatgt cccagttgtc gcccatggag
    25801 atcgcaccgt ggtcgaggat acgcagcatg tgaccgttgt tgttgatatt gtaggcaaat
    25861 ttttcgttat caaacgcgac gccagaaccc tgcgacagac ctttaccctg cgaggtcatc
    25921 gagtcagtag cgtactgaac aacaaacttg ttaaagccct tcaggacact ctgagtatgt
    25981 tcagcagtga ataaccagcc gtctttcgat gcgccatcaa ccagacgata gttatcacgc
    26041 aggttggcac gaccgtagtc gacacccagt tctaatgtgc cgcccgggtt gatttccatc
    26101 tgcgctaaac gcacatcgaa aacgtcgttc gcggtttcgt tggtatagtc ataaatattg
    26161 ttgctggcaa aagaggaaga accaccagct tcagaggagc gggttgctgc cagagagagt
    26221 ttaccgaagc caacatcgat gttttccaga ccggcaccag gaccagaaat atcccagtag
    26281 tagaagtcga tcatatgaac gtcatgacgt tggtagaagc gcttacctgc ccagatggtg
    26341 gaacctggca gccattcgat caggtttttt ccctgcacgt ttgcttcacg gaaggccgga
    26401 tcggtagctt cccagtcatt ctgttgcgcg acggaatagg ccacgttagt gtcgaaatag
    26461 aagctcttat cgccctcttt ccacacttcc tgacccaatt ttaattcagc ataagtttca
    26521 cattcgttgc caagacggta tttactttga gcaccggtag tctggaaaca ctgttgttca
    26581 ccgccgctac ctgtccagcc aataccggaa cgtgcatagc cgtggaaatc aacagccatt
    26641 gcctgagcag acattacgcc cgctgcgacg gcaaccgcca gaggaagttt gcgcagagta
    26701 atcatcattc tatctcctga gtcattgctt ttcttttttc acatcacctg tgacaggctt
    26761 tgtgtgtttt gtggggtgct taaacacccg gctccttatg cagtcgacga catgcagtgc
    26821 catcctcacg gaacagatgg caacgctctg gcggcaggcc gatagcgaat gtggcacctt
    26881 cttctaccaa caccacgtcg ttctggcggt acaccaggtt ttgacgaatg gaagggatct
    26941 ggatatagat ttgggtttcg ttgccgagtt gctcgacgac ctgaacttca ccctcaagga
    27001 tgacgtcagc gatatcactc ggcagtagat gttccgggcg aatacccagc gacatattgg
    27061 ctccaacctg gacatcacgg ctttcaacag gcagccagac ttgttgacga tttggcatcg
    27121 gcagctccac ctgcacttga tcgattgcgg tggcggtcac ttttaccggc aggaagttca
    27181 tctttggcga accgataaat ccggcgacaa aacggtctgc cggatagtgg tacagttcca
    27241 gcggtttccc aacctgtgcc acgcgaccgg cgtccagcac cacgattttg tcggccagcg
    27301 tcatcgcttc gacctgatcg tgggtgacgt aaatcattgt gcggcccagg cgtttatgca
    27361 gacgggagat ttcgatacgc atttgcacac gcagtgcagc atcgaggttg gagagcggtt
    27421 catcgagcaa aaatacgctt ggctcggcca ccagcgtacg gccaatcgcc acgcgctgac
    27481 gctgaccacc ggagagcgct ttcggtttgc gatccagcaa atgcgccagt tgcagcactt
    27541 ccgccacctg gttgacgcgt tggttaatca cctctttttt tgcgccagcc agtttcaggc
    27601 caaatgacat gttttctgct actgacaggt ggggatagag cgcgtaagac tgaaacacca
    27661 taccaacgcc gcgttctgct ggcggagtgt cattcatccg tttctcaccg atgaacaggt
    27721 cgccgctggt gatcgtctca agcccggcaa tcatgcgcag taaagtcgat ttaccgcagc
    27781 cagacggtcc gacaaacacc acgaattcac cttcatggat atcgagattg atatctttcg
    27841 ataccacgac ctcgccccag gctttcgtta cattttgcag ctgtacgctc gccatgccct
    27901 tctccctttg taacaacctg tcatcgacag caacattcat gatgggctga ctatgcgtca
    27961 tcaggagatg gcttaaatcc tccaccccct ggctttttta tgggggagga ggcgggagga
    28021 tgagaacgcg gcttctgtga actaaaccga ggtcatgtaa ggaatttcgt gatgttgctt
    28081 gcaaaaatcg tggcgatttt atgtgcgcat ccccacatta ccgccaattc tgtaacagag
    28141 atcacacaaa gcaacggtgg ggcgtagggg caaggaggat ggaaagaggt tgccgtataa
    28201 agaaactaga gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta
    28261 tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt
    28321 ccgcctcggc tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
    28381 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
    28441 tcaccgttga gcatccggat aaactggaag agaaattccc tcaggttgcg gcaactggcg
    28501 atggccctga cattatcttc tgggcgcacg accgctttgg tggctacgct caatctggcc
    28561 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacatggg
    28621 atgccgtacg ttacaacggc aagctgattg cttacccaat cgctgttgaa gcgttatcgc
    28681 tgatttataa caaagacctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
    28741 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
    28801 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
    28861 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
    28921 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
    28981 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
    29041 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
    29101 ctaaaccgtt cgttggcgtg ctgagcaccg gtattaacgc cgccagtccg aacaaagagc
    29161 tggcgaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
    29221 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
    29281 cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
    29341 agatgtccgc tttctggtat gccgtacgta ctgcggtgat caacgccgcc agcggtcgtc
    29401 agactgtcga tgaagccctg aaagacgcgc agactcgtat caccaagtaa tgctgtgaaa
    29461 tgccggatgc ggcgtgaacg ccttgtccgg cctacaaaac tgaaacgcat gtaggcctga
    29521 taagacgcat cagtgtcgca tcaggcagtt gttgtcggat aaggcgtgaa agccttatcc
    29581 gtcctggaat gaggaagaac cccatggatg tcattaaaaa gaaacattgg tggcaaagcg
    29641 acgcgctgaa atggtcagtg ctaggtctgc tcggcctgct ggtgggttac cttgttgttt
    29701 taatgtacgc acaaggggaa tacctgttcg ccattaccac gctgatattg agttcagcgg
    29761 ggctgtatat tttcgccaat cgtaaagcct acgcctggcg ctatgtttac ccgggaatgg
    29821 ctggaatggg attattcgtc ctcttccctc tggtctgcac catcgccatt gccttcacca
    29881 actacagcag cactaaccag ctgacttttg aacgtgcgca ggaagtgttg ttagatcgct
    29941 cctggcaagc aggcaaaacc tataactttg gtctttaccc ggcgggcgat gagtggcaac
    30001 tggcgctcag cgacggcgaa accggcaaaa attacctctc cgacgcgttt aaatttggcg
    30061 gcgagcaaaa actgcaactg aaagaaacga ccgcccagcc cgaaggcgaa cgcgcgaatc
    30121 tgcgcgtgat tacccagaat cgtcaggcgc tgagtgacat taccaccatt ctgccggatg
    30181 gcaacaaagt gatgatgagc tccctgcgcc agttttctgg cacgcagccg ctctacacac
    30241 tcgacggtga cggcacgttg acgaataatc agagtggcgt gaaatatcgt ccgaataacc
    30301 aaattggctt ttaccagtcc attaccgccg acggcaactg gggggatgaa aagctaagcc
    30361 ccggttacac cgtgaccacc ggctggaaaa actttacccg cgtctttacc gacgaaggca
    30421 ttcagaaacc gttcctcgcc attttcgtct ggaccgtggt gttctcgctg atcactgtct
    30481 ttttaacggt ggcggtcggc atggttctgg catgtctggt gcagtgggaa gcgttgcgcg
    30541 gcaaagcggt ctatcgcgtc ctgctgattc tgccctacgc ggtgccatcg ttcatttcaa
    30601 tcttgatttt caaaggattg tttaaccaga gcttcggtga aatcaacatg atgttgagcg
    30661 cgctgtttgg cgtgaagccc gcctggttca gcgatccgac caccgctcgc acgatgctga
    30721 ttatcgtcaa tacctagctg ggttatccgt acatgatgat cctctgcatg ggcttgctga
    30781 aagcgattcc ggacgatttg tatgagcctc agcaatggat ggcgcaggtc cgttccagaa
    30841 cttctttaag attacgctgc cgctgctgat taaaccgctg acgccgctga tgatcgccag
    30901 cttcgccttt aactttaaca acttcgtgct gattcagctg ttaaccaacg gcggcccgga
    30961 tcgtcttggc acgaccacgc cagccggtta taccgacctg cttgttaact acacctaccg
    31021 tatcgctttt gaaggcggcg ggggtcagga cttcggtctg gcggcagcaa ttgccacgct
    31081 gatcttcctg ctggtgggtg cgctggcgat agtgaacctg aaagccacgc gaatgaagtt
    31141 tgattaaggg agataacaaa aatggcaatg gtccaaccga aatcgcaaaa agcacgtttg
    31201 tttattactc acctgctact gctacttttt atcgcggcga ttatgttccc gctgctgatg
    31261 gtcgtcgcta tctcgctgcg tcagggaaac ttcgcgaccg gtagcctgat cccggagcaa
    31321 atctcctggg atcactggaa actggcgtta ggttttagcg ttgaacaggc tgatggtcgc
    31381 attacgccac cgccattccc ggtactgctg tggctgtgga actcggtaaa ggtcgctggg
    31441 atttccgcga ttggcattgt ggcgctctcc accacctgcg cctacgcttt cgcccgtatg
    31501 cgctttccag gcaaagcgac gctgctgaaa ggaatgctga ttttccagat gttcccggca
    31561 gtactttcac tggtcgcgtt gtatgcgttg tttgatcgtc tgggtgagta cattccattc
    31621 attggcctga atactcacgg cggcgtaatt ttcgcgtatc tgggtgggat tgcgctgcat
    31681 gtctggacca tcaaaggcta tttcgaaacc atcgacagtt cgctggaaga agcggctgcg
    31741 ctggatggtg cgacaccctg gcaggccttc cgccttgtcc tgttgccgct gtcagtaccg
    31801 attctggcgg tggtattcat cctgtcgttt atcgctgcca ttactgaagt tccggtcgcg
    31861 tcgctgttac tgcgtgacgt aaacagctac accctggccg tggggatgca gcaatacctc
    31921 aacccgcaaa actacctgtg gggtgacttt gccgccgctg ccgtgatgtc tgcattaccg
    31981 atcaccatcg tcttcttgct ggctcaacgc tggctggtca acggcctgac ggcaggtggt
    32041 gtgaaaggtt aaagatgttg ttctgccaat gttatgccgc tgcaccctca acttacgtta
    32101 tcccaacttg tgactattat tcggcgctcc acggagcgcc tttttttctt tcgtctgcaa
    32161 tctgaatcgt tcgccggtta atattcccat catagaactt attattttta cgttatttgt
    32221 tttcccactt acgataattc tctttcgtgc tctgagtcac ggcaatagta ttgttttcat
    32281 caatttttga taattatcac aattaagatc acagaaaaga cattacg
//
LOCUS       DADWTH010000040        31689 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.040, whole genome shotgun sequence.
ACCESSION   DADWTH010000040 DADWTH010000000
VERSION     DADWTH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.040"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(130..1650)
                     /locus_tag="JNY02_001951"
     CDS             complement(130..1650)
                     /locus_tag="JNY02_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB9433497.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKSKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            1741..2472
                     /gene="tonB"
                     /locus_tag="JNY02_001952"
     CDS             1741..2472
                     /gene="tonB"
                     /locus_tag="JNY02_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBB9433498.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRAL
                     SRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWR
                     YEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(2512..2910)
                     /gene="yciA"
                     /locus_tag="JNY02_001953"
     CDS             complement(2512..2910)
                     /gene="yciA"
                     /locus_tag="JNY02_001953"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBB9433499.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3015..3554)
                     /locus_tag="JNY02_001954"
     CDS             complement(3015..3554)
                     /locus_tag="JNY02_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBB9433500.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(3584..4327)
                     /locus_tag="JNY02_001955"
     CDS             complement(3584..4327)
                     /locus_tag="JNY02_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBB9433501.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4355..4444)
                     /locus_tag="JNY02_001956"
                     /pseudo
     CDS             complement(4355..4444)
                     /locus_tag="JNY02_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4684..5322
                     /gene="ompW"
                     /locus_tag="JNY02_001957"
     CDS             4684..5322
                     /gene="ompW"
                     /locus_tag="JNY02_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBB9433502.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5382..5888)
                     /locus_tag="JNY02_001958"
     CDS             complement(5382..5888)
                     /locus_tag="JNY02_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBB9433503.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(5934..6434)
                     /locus_tag="JNY02_001959"
     CDS             complement(5934..6434)
                     /locus_tag="JNY02_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBB9433504.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6520..6699)
                     /locus_tag="JNY02_001960"
     CDS             complement(6520..6699)
                     /locus_tag="JNY02_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBB9433505.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(7080..7886)
                     /gene="trpA"
                     /locus_tag="JNY02_001961"
     CDS             complement(7080..7886)
                     /gene="trpA"
                     /locus_tag="JNY02_001961"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBB9433506.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(7886..9079)
                     /gene="trpB"
                     /locus_tag="JNY02_001962"
     CDS             complement(7886..9079)
                     /gene="trpB"
                     /locus_tag="JNY02_001962"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBB9433507.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(9091..10452)
                     /gene="trpCF"
                     /locus_tag="JNY02_001963"
     CDS             complement(9091..10452)
                     /gene="trpCF"
                     /locus_tag="JNY02_001963"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBB9433508.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(10453..12048)
                     /gene="trpD"
                     /locus_tag="JNY02_001964"
     CDS             complement(10453..12048)
                     /gene="trpD"
                     /locus_tag="JNY02_001964"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBB9433509.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(12048..13610)
                     /gene="trpE"
                     /locus_tag="JNY02_001965"
     CDS             complement(12048..13610)
                     /gene="trpE"
                     /locus_tag="JNY02_001965"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBB9433510.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(13632..13724)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            13883..14764
                     /gene="yciV"
                     /locus_tag="JNY02_001966"
     CDS             13883..14764
                     /gene="yciV"
                     /locus_tag="JNY02_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBB9433511.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            14761..15381
                     /locus_tag="JNY02_001967"
     CDS             14761..15381
                     /locus_tag="JNY02_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBB9433512.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKS
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15482..16354
                     /gene="rluB"
                     /locus_tag="JNY02_001968"
     CDS             15482..16354
                     /gene="rluB"
                     /locus_tag="JNY02_001968"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBB9433513.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16394..16984)
                     /gene="cobO"
                     /locus_tag="JNY02_001969"
     CDS             complement(16394..16984)
                     /gene="cobO"
                     /locus_tag="JNY02_001969"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBB9433514.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(16981..17739)
                     /locus_tag="JNY02_001970"
     CDS             complement(16981..17739)
                     /locus_tag="JNY02_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBB9433515.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            17959..19008
                     /gene="sohB"
                     /locus_tag="JNY02_001971"
     CDS             17959..19008
                     /gene="sohB"
                     /locus_tag="JNY02_001971"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBB9433516.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     AWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(19044..19295)
                     /locus_tag="JNY02_001972"
     CDS             complement(19044..19295)
                     /locus_tag="JNY02_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBB9433517.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            19675..22272
                     /gene="topA"
                     /locus_tag="JNY02_001973"
     CDS             19675..22272
                     /gene="topA"
                     /locus_tag="JNY02_001973"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBB9433518.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVIDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            22482..23456
                     /gene="cysB"
                     /locus_tag="JNY02_001974"
     CDS             22482..23456
                     /gene="cysB"
                     /locus_tag="JNY02_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBB9433519.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            23751..23915
                     /locus_tag="JNY02_001975"
     CDS             23751..23915
                     /locus_tag="JNY02_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBB9433520.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            23918..24085
                     /locus_tag="JNY02_001976"
     CDS             23918..24085
                     /locus_tag="JNY02_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433521.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            24199..24294
                     /locus_tag="JNY02_001977"
     CDS             24199..24294
                     /locus_tag="JNY02_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433522.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            24458..27133
                     /gene="acnA"
                     /locus_tag="JNY02_001978"
     CDS             24458..27133
                     /gene="acnA"
                     /locus_tag="JNY02_001978"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBB9433523.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTQYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(27197..27787)
                     /gene="ribA"
                     /locus_tag="JNY02_001979"
     CDS             complement(27197..27787)
                     /gene="ribA"
                     /locus_tag="JNY02_001979"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBB9433524.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            27957..28721
                     /gene="pgpB"
                     /locus_tag="JNY02_001980"
     CDS             27957..28721
                     /gene="pgpB"
                     /locus_tag="JNY02_001980"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBB9433525.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            28870..29178
                     /gene="lapA"
                     /locus_tag="JNY02_001981"
     CDS             28870..29178
                     /gene="lapA"
                     /locus_tag="JNY02_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBB9433526.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            29185..30354
                     /gene="lapB"
                     /locus_tag="JNY02_001982"
     CDS             29185..30354
                     /gene="lapB"
                     /locus_tag="JNY02_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBB9433527.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAVADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            30547..31284
                     /gene="pyrF"
                     /locus_tag="JNY02_001983"
     CDS             30547..31284
                     /gene="pyrF"
                     /locus_tag="JNY02_001983"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBB9433528.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            31284..31610
                     /gene="yciH"
                     /locus_tag="JNY02_001984"
     CDS             31284..31610
                     /gene="yciH"
                     /locus_tag="JNY02_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBB9433529.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         7943 a         7699 c         8136 g         7911 t
ORIGIN      
        1 tcacagcctc agctaatgag cgagcttcaa aagtgcttaa gtcgggtaat gctttacgga
       61 agtactctgt gaatccgaat aagaagggcc ttattgagac ataaaaaggg gcttcatagc
      121 ccctgaaaat tacagttgat aagcatcaat atccagtagc tctttatcag caatttcaaa
      181 gattggtgac ccatcattta gcggtagcgg gtctgtaggt atggtgattt cagtgtcgtt
      241 ctgtagcgtt atagttactg aacccttacc caatgtaagc ccttttagat gtttggacaa
      301 aattaattga cactcgacac gacccgcttt agttgtcagg tcaatatctt taagctgggg
      361 tagttcctta tcagtgagtg aagccttacg cttcatagta tcaatcgtgg tctgtagttc
      421 tgtgcgcttc tggttaagtt ccttaagctg aatttttagt tcatcaatat catccatctc
      481 aagaagtagg gcggtagctt tcttgattct ggctgtgaca atttcaacct catgctgtaa
      541 cgctgcaatc ttcctctctg attctccatc attccccacg tcaatcatcc ccagaaaatt
      601 atcaatcatc catcggtcaa acggtttacg cttcaacggt ggaacatcac agcgaccgtc
      661 taagtggtta ttgcacctgt agaccccagc atacttactc tttgttccgg taggatggac
      721 actagcccca cactgacacc taaacagacc tttgaagatg ttaatcgtta gtgggttgtc
      781 acttgcaggt gtacggcctt tacgattctt atcaagaatc tcttgagctt tattgaacgt
      841 tgcagcatct atagcggctt cgtagtagtt cggtatagcg gggcggtcat tgcgctgtga
      901 tgctggtagt gtacctatcg ttgtctctga ccttaagata gtacgcacag aatgaacgct
      961 aaatccatta gcatctttac gtcgtgagaa tgaactaatg ttattagcct gtaggtgacg
     1021 tgcaatgtta tgcagacttt caccatctag cagcagcttg aaaatcaagc gtacagtttc
     1081 agcatgttca ttgagtttaa aagtctcagg gtcaatccat ccgggaacat tccttgttaa
     1141 acgttgttca ccagcaaggg caagctttgc acgttgttcc catttctgac taacacggaa
     1201 agacttctgt ttactctcct gatgcgctat gtagctttta acccgctgaa tgattgagat
     1261 tcctaaatca tcggatagag catcccttgt gtagattagc ccatcctgta gagtgtgaat
     1321 ctcgatacca tgagcaataa tgcgattcat caaggcttct gtttcaagat gttcaaggcg
     1381 gcctatgcgg tcgatagctt cgactagtaa aattgtccct tctggaaact cccctgattc
     1441 aatgcgggag agcatatccc caaattgccc tgataagtgt ttacctttgt aaccggatag
     1501 accttcatca ttgaaagatg ccactacctg agcatcgcta ttcttcctta accactgttt
     1561 gaatagctca ttttgtcgtc tggttgaatc agcaccttca gcaccttgtg tggctgatga
     1621 gaatctcaag tatgttatta cggttctcac ggtgataaac tcccatgcaa ttgattatca
     1681 tttaaagatg aacttataaa cgttatggct aatgctacag tgccgaatag ctacaataca
     1741 atgccccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat
     1801 ggtgctgttg tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg
     1861 cctgcgcaac caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc
     1921 gttcagccgc caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa
     1981 ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg
     2041 aagccaaaac cgaagccggt gaaaaaggta caggagcagc caaaacgcga tgtcaaaccc
     2101 gtagagtcgc gtccggcatc accgtttgaa aatacggcac ccgcgcgccc cacatcaagt
     2161 acggcaacgg ctgcaaccag caagccggtt accagtgtgg cttcaggacc acgcgcatta
     2221 agccgtaatc agccgcagta tccggcacga gcacaggcat tgcgcattga agggcaggtt
     2281 aaagttaaat ttgacgtcac gccggatggt cgcgtggata acgtacaaat cctctcagcc
     2341 aagcctgcga acatgtttga gcgtgaggtg aaaaatgcga tgcgcagatg gcgttatgag
     2401 ccgggtaagc caggcagtgg gattgtggtg aatatcctgt ttaaaattaa cggcaccacc
     2461 gaaattcagt aagcagaaag tcaaaagcct ccgaccggag gcttttgact attactcaac
     2521 aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca tacttaaata atgcttctgt
     2581 cgctttatag cgttgcccga ttggttcaga cgctactttt ttcacccaca cttcaatatt
     2641 aatgctgacc gatgtcgtcc ctttctggac acagcgtgca tagcagcaca ccacatcgcc
     2701 gaccgcaacc ggccgtaaga aagtcattcc ttcaacccgc acagtcacta cgcgaccgtg
     2761 ggcaatttct tttgccagaa tagcgccgcc aatatccatt tgtgacatta accaaccacc
     2821 aaagatgtca ccattggcat tggtatcggc gggcatggct aaagtacgta aaacaagatc
     2881 gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg acttaaaagg aaaatcacag
     2941 gcgcgatgct actatgattt tcctgaggag aacagaggag atacagcgcc agccccgaag
     3001 gactggcagt ctggttagga tttatcttcc tgcggcatgt ggcggtagat atagataccg
     3061 cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa agactttaaa gttgacccaa
     3121 atattttgcg gcagccagaa tgcgatgtag atgtttgcca gaccgcaaag gataaagaaa
     3181 acagcccagg ccagatttag cttcgaccat accggttgcg gcagcgtgag ttctttaccc
     3241 agcatccgct gaattagcgg ctttttcatc acccattggc tgactaacag ggctaccgca
     3301 aacagagcat aaatgactgt aaccttccat ttaataaact catcattgtg gaagaacagc
     3361 gtcaagccac cgaagacgac caccagaaca aaagtgatca gggccatctt ctcaacctta
     3421 cgaaagcgaa cccagctata gataagcaca atcgccgtgg cgacgatcag cgctgcagta
     3481 gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg taaaaaatca
     3541 agaaactgct tcattttacg attccgtaat caaatgctta aggttattgg cgaatcaaca
     3601 tatacaggcg gaacagatag atgagcaata tggcagaaat caggttgctc aaggtgttcg
     3661 ccagtacggc accaatttcc ggggttaatg cggcaaaaga agaggcaaaa agcagcaaca
     3721 gtgtttttgc cagcaaccag ctcagtactg cgggtgccac cagacgcata ttcgcccaag
     3781 tcagccgcat actgctacgc atcgaggcaa aaatgcccat tttgtcctgt accagcatca
     3841 ccggtgccag ggccagtaaa atggccatga taattcccgg aacgaccacc agcatgatgc
     3901 caatctgtac taaaagggta gtcagaaaaa tcagaataaa taacttgggc aatatcggcg
     3961 cactggcacc aatagctcgc agcgcacaga ctctctgacc cgcagacacc agctggataa
     4021 ttaatattac gcctccggcg agaatggcgt taccgattaa ttctgaaaaa gtggacgccg
     4081 ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat attctgaacc aggtcgaaca
     4141 acccactact gccgctaacg ggcacgccgt cattgagctg cgcaagctgt gcatcactgg
     4201 gtgagaaaac atgccctaac accactgtga taaacgcaca tagcaacgat accaacagaa
     4261 tggtcataaa ttgattacgg aagaaatttc cggtgtcacg gtatacggac tgcgccgtga
     4321 tagacatgca ctctccttga gttttatgca ggtgttaatt agcgggcaat tgtaccctgg
     4381 ttaagccttg ggtggcagca tcaggtcttg tatggaaaag catatctttg taaagcggag
     4441 gtaattgctg gccttcaggc gtgggcaaac cgcggcaagt ggcgatcgag ttaatatttg
     4501 cgtagagaaa atatttaaaa attgatttaa atcacattaa ccaggattct caatgcaact
     4561 tctaaattaa tccagatcaa taaatggtga attatcatat gtaatgtgat ctatgtagga
     4621 tcatttgtta ctccaatgta ggtatattcg tcacgttttt ataaccataa cgacggagcg
     4681 gatatgaaaa agttaacagt ggcggctttg gcagtaacaa ctcttctttc tggcagtgcc
     4741 tttgcgcatg aagcaggcga attttttatg cgtgcaggtt ctgcaaccgt acgtccaaca
     4801 gaaggtgctg gtagtacgtt aggaagtctg ggtggattca gcgtgaccaa taacactcaa
     4861 ctgggcttga cgtttactta tatggcgacc gacaacattg gtgtggaatt actggcagcg
     4921 acgccgttcc gccataaaat cggcacccgg gcgaccggcg atattgcaac cgttcaccat
     4981 ctgccgccaa cactgatggc gcagtggtat tttggtgatg ccagcagcaa attccgtcct
     5041 tacgttgggg ccggtattaa ctacaccacc ttctttgata atggatttaa cgatcatggc
     5101 aaagaggcgg ggctttccga tctcagtctg aaagattcct ggggagctgc cgggcaggtg
     5161 ggggttgatt atctgattaa ccgtgactgg ttggttaaca tgtcagtgtg gtacatggat
     5221 atcgatacca ccgccaatta taagctaggc ggtgcacagc aacacgatag cgtacgcctc
     5281 gatccgtggg tgtttatgtt ctcagcagga tatcgttttt aattccgcac aaaaacgacc
     5341 ccgtaatata cggggtcaat aaggacatgg tataaagggt attatttctt cgcttctacg
     5401 ccatcagttt cagaacgaat taaaaatttc tcagttgttt gcggaatatg ctgaatcagc
     5461 cagtcggcca tttgcttttc ctcatttaaa atcgcttcga tgatcggaat tgagactgta
     5521 tcaccggcat tttttgctgc tgctaatagt gaggtgtaac aggcgatttc aaattgctcg
     5581 aagacatatc cgctaataga gcctttaact atttcatcag aagggaatat accaccgatt
     5641 gactgcccaa gcgcagccat tttactcatg gaatctttaa tgactgaacg tgaaatgtca
     5701 ttacgatcaa gaatagtttc cagttgaaca atctggtttt tggtttcact aagatgttgt
     5761 tcaatacgag cgcgtagttc aggataatta tctatacggc tggccatgga ttcaagcata
     5821 gattcggctt gcttttccat tgcgtgggcg tcacgtaacc agtcatgata atgttcaata
     5881 cgattcatga aaatgtcctc tttataaaat gactaaaagt taaaattcat atttcaggct
     5941 ttattttcgg ctttcttatt tacgttatta agggccagat cagtcagttt gatgtcggtg
     6001 gccttttctt cttccagggt ttctttcaga agcttcgctg ctttacggta acctaattgt
     6061 tcagccagcg tcgctaatgt cccgtaactg gcaatctcat aatgctcgac tttctgtgct
     6121 gcggcaatca gtgcggcatc acgcacttcg tttttctcgg tactttcgat gacctcatta
     6181 gcttcttcaa taagaccttc cattgccaca catttcatgc gcttaatttt cagatttgat
     6241 tctgattcca caacttgatc aattcgttca atctgtccat gagtttcctc gaggtgcgca
     6301 tgaaaagcct gacttaattt ttcatttgat gttgctcttg cgagttttgc cagtgcccgg
     6361 ggtaattgtt tttctgcgct gtaggtatct gaaagcagtt gaataaatac atcttcaatg
     6421 gtcttcatat tcatattttt ctccagtgaa atcactgcga gccatacggc ccgcagatgc
     6481 aattatcttt atcagctgat attaataatt aacggtgaat caggatttgt ctgatttatt
     6541 accaccgctt tgttgaccgc cttttttacc cgcttcagat gcgcgttgcg gatcattttt
     6601 aaaattaccg ccgctatgct gaccgccttt acggcctgcg tcggatgcct tctcacggtc
     6661 ttcggcgaaa tttcctgaac caccacgatg ttcggccatg ttatttctcc cgttgcgttg
     6721 cattgtttca ttaatatgag tgttgtgtgt cgacactcat taaaattagt cgctaatgag
     6781 aattagtcaa attaagcgca acgagaagat agagggaaaa tatattttga ggaacattct
     6841 ggatatatta acaattacct gaggaataaa tgacttagag aaaattattg attttactgg
     6901 tgttatgttg cgggattaat ttgtttgatt atattactgt tgggcggaaa aatgacgtaa
     6961 gttgacgttc gaccggggta agcgaaacgg taaaaagata aatattaaat gaatttagga
     7021 tttttccggc ttcattaaag aaagttaaaa tgccgccagc ggaactggcg gctgtgggat
     7081 taactgcgcg tcgccgcttt catcggttgt acaaaaactt tcagtgccgc cagcattttc
     7141 tctggctcat taatatgttg ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc
     7201 gcagctcctg catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaacccctgc
     7261 aatggaggtg cagcgttgta ctctttcagc ttcgcaacca gatgattgag gggtaacgcg
     7321 gcgcggtttt ctgcgccggt cacgcccgct cgtgacagca aatatgtgta accacgaccg
     7381 taagaggcta tctggcgcag caggtcgtca tcggcatttg gcgggcagat gaagataggt
     7441 gcgacattat ggcgcaacgc ggcctggcgg aagggcgcgg actcttcaac tggcacatcg
     7501 gcaaccagca ccgaatcgac gccgactttc tcgcactggg cataaaactc atcaatgcct
     7561 ttgttaaaca ccagattggc atacatcaac aggccaatgg gaatggtcgg gtgtttctgg
     7621 cgaatcagtg ccagcatttc aaaacattgt gccggagtca cacctgccgc aaaggcgcgc
     7681 agagtggcgt tttgaatggt cgggccatcc gccagtgggt cggagaaggg gatacctaac
     7741 tccagcgcgt cagcaccggc ttcaattagc gtatcgataa ttttcaatga ctgctcaatg
     7801 cccggatcac cgagcgtgac gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg
     7861 gcaaacagag attcgtagcg ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa
     7921 cggtgaagat gtctttatcg ccgcgaccgg aaaggttaac caccagtagc tgctctttat
     7981 ccgggttttc gcgcatcatt ttcagcgcat gggccagggc gtgggaggat tccagcgccg
     8041 ggatgatccc ttcgtgcagg cacagcgttt tgaaggcttc aagggcttca tcatcggtaa
     8101 tagacacgta atcagcgcgt ccagtgctgt taagatacgc gtgttgtggg ccgacagacg
     8161 ggaaatccag tccggcggag atggagtaag attcttcaat ctgcccgtct tcggtttgca
     8221 tcatcggcgc tttcataccg aaataaatgc ccacgcgacc atgttttagc ggtgcgccgt
     8281 gctcgccagt ttcgataccg tgaccaccag gctccacacc aatcaggccg acgttggttt
     8341 cattgatgaa atcagcaaac atgccgatgg cattcgaacc gccgccaaca caggcgataa
     8401 cggcatccgg caggcgacct tctctttcca gaatctgcgc tttggtttct tcgccaatca
     8461 tccgctgaaa ctcacggaca atggtcggat aaggatgcgg gccagccgcg gtgcccagca
     8521 tatagtgcgc ggtttcataa ctgccggacc agtcgcgtag cgcctcatta caggcatctt
     8581 tcagcgtggc ggaaccgcta tgtaccggga tcacttccgc acccattaag cgcatacgaa
     8641 aaacgttagg cgactggcgt tcaacgtctt tggcacccat ataaatacgg catttcaggc
     8701 cgagcagggc gctggcaagg gccgacgcca cgccatgctg accggccccg gtttcggcga
     8761 tgatttcggt tttacccatc cgcttcgcca gcaacgcctg ccccagcacc tggttggttt
     8821 tatgtgcgcc gccgtgcagc aaatcttcac gcttgagata cagcgtggtg ttcgtcccgg
     8881 ctgtaatgtt ctggcatttg gtcagtgcgg ttggacgccc ggcatagttt ttcagcaggt
     8941 cgttgaactg ggcttgaaat tcaggatctt tttgcgcgct gacaaaagct tcttccagct
     9001 ggcgcagagc aggcatcagg atttgtggca cgtacatgcc gccaaactca ccaaaatagg
     9061 ggttaagtaa tgttgtcatt gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga
     9121 ggccaaaaga cgtgcgtctt tgatgcccgg ttgcgactct acagcagaat taaaatcaag
     9181 tccggcgcag ccggtttgtg ccgcttccac gcagttatct gcgcctaagc cccccgccag
     9241 cagaacgttg ccaagcgatt gaccatttaa tagtgaccag tcaaaacgtt gcccgcttcc
     9301 accctggccg ttgtctaaaa catatttatc aacgtgctga aactcgcggg cgggcagggt
     9361 ttcaccgacg cttaatgctt tccagatggc aacatgtgct ggcagagctt cacgcagcgt
     9421 atcgatatac agctgatctt cattaccatg cagttgcact gccgccagcg ataacacctt
     9481 agctttgtcc accacatcgg caatatcgtg attgcggaac acgccaacat actgcaacgg
     9541 tgccgcagcc atcacttcct gcgcctgttc aacgttgacg caacgcggtg atgtcgcaac
     9601 aaaaatcaac ccaccgtaaa tcgcgcccgc gtcataagct gctttagcat cttgcccacg
     9661 cgtcaggcca catactttat tctcacccag caacacccgg cgcacggcgg cgtgcaaatc
     9721 gtcatgggcc atcaacgccg aaccaatcag aaaaccgtta gcgaagtggc ttaactcgcg
     9781 cacctgagcg taagtattga tgccggattc gctgattacc gttacgttgt gccccagttt
     9841 cggcgcaagc tcgcgggtac ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt
     9901 gatgccaacg acctttgccc ctaatgcaat ggcgcgctcc agttcctctt cattactgac
     9961 ttcggtcagc acccccatct ccagactgtg agcgacggct gcaagctggc gatattggtc
    10021 gtcatccagt actgaaagca ttaataagca ggcatcggcc tggtaatagc gcgccagata
    10081 gatctggtaa gggtcgataa tgaagtcttt acataaaatc ggctgcgggg cgatttggct
    10141 gacgatgagg aggaaatcaa agctcccctg aaaatatttc tcatcagtca gcaccgaaat
    10201 tgccgaagcg taatgtttat aaatggcggc aatgcgtgcc ggatcgaaat catcacggat
    10261 cacgcctttt gacggcgacg cttttttaca ctccagaata aacgccgtgc gtgcaccctg
    10321 tagcgcatca taaaaatgtc gtgtgctcgg ctgaacctca ttctgaaaac tggccagcgg
    10381 ttgctgctgt ttgcgggttt ctacccaaat cgccttgtct gcgacgattt tcgctaaaac
    10441 ggtttgcatc atttaccctc gtgcagccag cgcggtaact ctgtcgtaag cggaaccact
    10501 gcgcagtacc tcaagaacgg tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg
    10561 cattaacatg gcgacgttcg cagcgacggc tgcttcatgg gcggcgtcgc ctttaccttg
    10621 taacaagcgt gttaaaatgt cacggttttc ttcaggtgtt ccgcctgcca gttgctcctg
    10681 gtggtaggga gtcaggccaa aatcttcagc ggtcaattga tagctcttaa ttttgccgtc
    10741 atgcagctcg gcaacgattg tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg
    10801 caccaccgcc gcgcgttgat accccagcac acgcaaggtt tcggcaatcg gcagcaccag
    10861 ttccggacta taaacaccaa ttaacgccag cggcggatgc gccgggttaa tcaatggccc
    10921 cagcacattg aacagggtgc gggttttcag ttgctggcga accggcatcg cgtggcggaa
    10981 tccggtgtga tatttcggcg caaagaggaa acatacgcct aactcgtcca gcgcctggcg
    11041 cgatttattg gcgttcatat caagattaat accgaatgcc gccagcagat ccgacgagcc
    11101 ggatttactg gagacgctgc ggttaccgtg tttcgccact ttcagcccac aggccgcggc
    11161 gacaaacgca ctggcggtag aaatattgat actgttgctg ccgtcaccgc cagtaccgac
    11221 gatatcagca aacagataat ccgggcgcgg gaacggcgct gcgttttcca gtagcgcggt
    11281 tgctgccccg gcgatctcgt tcgggtgctc accgcgaatt ttcatgctca ccagcgccgc
    11341 cgccagttgt tccggcttca gctcgccacg caccaccgct gaaaacagct ggtggctttc
    11401 ttgttggcta agcgtctgcg cctgatacag tttttccaga atcggttgca gcgtgttggc
    11461 tggctctagt ttctgctgcg cccaggccag cgtttgttcc agcaggcgag cgccctgggt
    11521 ggtgagaatg gattccggat ggaactggaa tccacaaacg cgatccgcat cgtgacgtac
    11581 tgccatcacc atgccattaa aatgggcgtt gatggttaaa ccggccggaa tgttactgcc
    11641 aaccagcgag tgataacgcg ccaccggcag cgggtttgtt aatccggcaa acatcgcctg
    11701 accgtcatgt tcaatgctcg acgctttacc gtgaagaatt tcgcccgcct gaccgacata
    11761 gcccccgtaa gcttcgacaa ttgcctgatg tccgaggcaa atgccaataa tgggcagctt
    11821 gccacgcaag cgggtgagga gttccggcat acaaccggct tcgctcggca caccggggcc
    11881 aggagaaagc atcagtaccg gattgctcat cgtcgccagg cgttcaatta aggtctgcgc
    11941 cggaatatgg ttgcggtaaa tcaccacgtt atgaccattg ctgcgcaact gatctgccag
    12001 gttgtacgta aaagagtcga tattatcgag cagcagaatg tcagccatca gaaagtctcc
    12061 tgtgcatgat gcgcggtggc aatagcgcgc agtacagcgc gggctttgtt acgggtttcg
    12121 tcggcttccg actgcggaac agaatcaagg actacaccag cacccgcttg cacggtggcg
    12181 ataccgtttt ccaccagcgc cgagcggatc acaatgcagg tgtcgagatc gccatgcgcg
    12241 gtgaaataac ctaccgcgcc gccgtagctg ccgcggcgac gaccttccgc ctcggcaatt
    12301 aactgcatag cgcgtacttt cggcgcaccg cttaacgtcc ccatattcat acaggcgcga
    12361 taagcgtgca gggcgtcaag atcgtgacgc agttcgccga ctacgcgaga gacgaggtgc
    12421 atcacatagg aataacggtc aactttggtg agatcggcga cgtagcggct gccaggggtg
    12481 caaatgcgtg ccagatcatt acgggcgaga tcaaccagca tcagatgttc agacagctct
    12541 ttatggtcag tacgcatttc cagttcaata cggctgtcga gatctctgtc cagtgaacca
    12601 tcggcgcgac gaccgcgtgg gcgtgttccg gcaatcgggt agatctcaat ctggcggctg
    12661 gtggcatcat acttgagcga gctttccggc gacgcgccaa atagggtgaa atcattatcc
    12721 tgcataaaaa acatgtacgg gctgggatta ctctttttca gcacgtaata ggccgccagc
    12781 ggtgacgggc agggcagaga gaaacggcga gatggcacca cctggaaaat ttctccagcg
    12841 cgaatcgctt tttgcaacaa acgcactacg ccaccgaact cttcatcgct ctgattacat
    12901 tcacaacgca tatgcggcac ggaaaccact ggcagcggcg gcgcggcttc ggtcagttgc
    12961 tgacgtagtt cgttcaggcg agcagtaaga cgttgttttt cttcttcatt cggagcaaac
    13021 aggctggcct gaatacgggt gctttttttc tgatggtcaa tcaccatcag cgtttcagcg
    13081 agataaaaac agaaatcagg gcagttattt tccgctgaca gttgcggtaa atcttcaaat
    13141 cccgccacaa ggtcataaga gaacaggccg ccgaagaaca ttgcttctcg ttcttccttc
    13201 ggtacattca acagattctg caataaacgg aaagcgtcaa aaaccgaaag ggagcataag
    13261 cgggcgtctt catccagcag tggactgaca ggggggaagc gcagcacacg gcagtttggt
    13321 gattgttcac tttccacacc cgcaggcagg gcgttatcca gtagtgccag cagggcttcg
    13381 ccattgccgg aaagtgcctg aattgtgacg gtgtcaccta atgctgtaat gtgcagcgca
    13441 ctgtctatca gcagcaggct ttttaaatca tctttgctgt cgatatctgc ggattccagc
    13501 agcagcgttg ccggacgatt cccacacaac tggtgaaaaa gcgcagtcgg gttgtcgcga
    13561 taagcgcctt cgcaggttag cagttcgaga gtcggttttt gtgtttgcat tgttattctc
    13621 taattttgtt caaaaaaaag cccgctcatt aggcgggctg ggtatctgat tgctttacgc
    13681 atggtgaata cactgcccgt ttcaggaagt ggccaccaac ctttcagtac gaaaattgct
    13741 ttcattgtcg ataccatttt tacgtgaact tgcgtactag tcaactggtt cgatgattaa
    13801 ttgtcaacag ctcatttcag aatatttgcc agacccgtta tgatgtcggc gcaaaaaaca
    13861 ttatccagaa cgggagtgcg ccttgagcga cacgaattat gcagtgattt acgacctgca
    13921 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
    13981 cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg caatcgcgcc
    14041 agcaagagag gaaatttcac gttcgggact ggcgctgaat cttattcccg gcgtggaaat
    14101 ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca
    14161 tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagca
    14221 aattgccgaa agacttgaaa aagcgcaaat acctggggcg ctggaaggcg cacaacgact
    14281 ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt ttccttgtgg agtgcggcaa
    14341 agccagttca atggcagatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt
    14401 tccgccacag tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa
    14461 ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt
    14521 tgcgcatttt gccgaacacc acggcgacgc gatggaagtc gcgcagtgcc agcaatcgcc
    14581 caatgaacgc acccagctgg ccacacttgc gcgtcagcat catttatggg catcacaagg
    14641 atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc
    14701 aggcgttgaa ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt
    14761 atgagccagt ttttttatat tcatcctgat aacccgcagc aacgtctgat caaccaggcg
    14821 gtggagatcg tgcgtaaagg cggggtgatt gtctatccaa ctgattccgg ctatgcgctc
    14881 ggctgtaaaa gtgaagataa aaacgccatg gagcgtattt gtcgtattcg ccagctgccg
    14941 gacggtcaca actttaccct gatgtgtcgc gatctttctg aactctcgac ctattcattc
    15001 gtggataacg ttgcgtttcg tttgatgaaa aacaacacgc cgggcaacta taccttcatc
    15061 ctgaagggga cgaaggaagt gccacgccgt ctgttgcagg agaaacgcaa aaccatcggt
    15121 atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg aagcactggg cgaaccgatg
    15181 ctttccactt cgctaatgct gccaggcagc gaatttaccg aatccgatcc cgaagaaatt
    15241 aaagaccgtc tggaaaaaca ggtagatttg attattcacg gcggctatct cgggcagaaa
    15301 ccaacaacgg ttattgatct taccgatgat acgccggtcg tggtgcgtga aggcgtaggt
    15361 gatgtgaagc ctttcttata agtgcgtaac tctgtatact acgcggcctt aaaatggcgc
    15421 tatacggcgc cattgattct cctgttcgac gcctgtgaag gcgacaccaa aaggaagctc
    15481 tatgagcgaa aaattacaga aagtgctggc ccgcgctggc cacggctccc gccgtgaaat
    15541 cgaatctatt attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga
    15601 tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat ggtcatctga tttcggtacg
    15661 tgagtccgct gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg
    15721 tacccgtaac gatccggaag ggcgtccgac ggtgtttgac cgtctgccaa agctgcgtgg
    15781 cgcacgctgg attgccgtag gtcgtctgga cgttaacacc tgtggtctgc tgctgttcac
    15841 caccgatggt gaactggcaa accgtttaat gcatccaagc cgtgaagttg aacgtgaata
    15901 cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt
    15961 gcaactggaa gatggtccgg cagcttttaa aaccatcaag ttcagcggcg gcgaagggat
    16021 caaccagtgg tacaacgtga ctctgaccga aggccgtaac cgtgaagttc gtcgtctgtg
    16081 ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt cgttacggtg atatcccact
    16141 gccaaaaggt ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct
    16201 gcgcgaactg gtggagcttc cgccagaaac cagctctaaa gtcgctgtag aaaaagaccg
    16261 tcgtcgcatg aaggctaatc agattcgtcg tgcggtgaaa cgtcacagtc aggtgagcgg
    16321 cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag tatgccggac gtcatatccg
    16381 gcatttttac agattaataa tcaatcccta tttgcgcctt aacaccggca tcaaacgcat
    16441 gtctgatggg gcgcagctca cttaccgtat ccgccagttc aagaatatcc cgatgacagc
    16501 ctcgacccgt gataatcacc gtctgttgat gtggacgttc atttaacgcc tgcaccactt
    16561 cttccagtgg caaatagtca tacgccacca tatacgtcag ttcatcaagc aaaaccatat
    16621 ccagtgagga atcagcaagc atccgctttg catgttgcca gacttcgcgg caggcggcgg
    16681 tatcagactc gcggttttgt gtatcccagg taaagcccgt tgccatcacc tgaaactcaa
    16741 cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca ggtgccttta ataaactgca
    16801 cgacgcccac tttttttccg tgaccaactg cgcgtgttgc cgtaccaaat gccgcggtgg
    16861 ttttgccttt tccattgccg gtaaagacga tgataatacc gcgttcatcc tgggcctggg
    16921 ccacacgagc atctactttt tctttcactc gctgctgacg ctgttggtag cgttcatcac
    16981 tcattgggaa attcctggtt tacggcccgg ttgggcgtca aaggtcatgc cggttttacg
    17041 gcggctgtca tcgcccatca gccagaggta gagcggcatg atatcagcgg gtgttttaag
    17101 tttctgtgga tcttcggtcg ggaaggcgct tgcgcgcatt gcggtgcgcg taccgcctgg
    17161 gttaatgcag ttgacgcgca ggcgctgctg atattcatcg gccagtacct gcatcatccc
    17221 ttcggtggca aatttcgacg ctgcatatgc accccagttg gctcgtccct gacgtccaac
    17281 gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg agtaataaag gaagcagtgc
    17341 ctgggtgagc ataaaggtgg cattaacgtt gacctgcatg acgtcctgcc agacctgcgg
    17401 attttgttcg ctcattgggc aaacatcgcc gagcaatccg gcattatgca aaacaccatc
    17461 cagacgcgga taattaacga caatgcgctg tgccagttgt tggcaatttt cggacgtgca
    17521 ggtcagcaaa tcgagaataa accactgtgg ctgacgccca gtttcttcgt ttatgtggct
    17581 ggctacctga cgtaattttt cttcattacg gcccaacaga atcactgtcg caccatagcg
    17641 tgcatacgtc atcgcggctt cacgaccaat cccatcgctg gctcccgtca ccaggataat
    17701 gcgatcattg agtaaatctt gttttggctg gtaatgcatg gctactcctc aacgacgttg
    17761 tctgtcgcgc tgccagtgta ctttatgact ttggggcttt atgcctgaaa cagaagggca
    17821 tttcaatcag cctggggcga taacaccgca aaatggatac tttgtcatac tttcgctgca
    17881 ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa ctgtgagcca aagcgttgtt
    17941 taaccaaggt ggggactcgt ggaattgttg tctgaatatg gtttgttttt ggctaaaatc
    18001 gttaccgttg tgctagcgat tgcggcgatt gccgccatta ttgtcaatgt tgctcaacgt
    18061 aataaacgcc agcgtggcga gttacgggtc aacaatctca gcgaacagta taaggagatg
    18121 aaagaagaac tggccgcggc gctgatggac tcacatcagc aaaaacagtg gcacaaagcg
    18181 cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag cgaaagccaa actgggcgag
    18241 gtggcaactg acagtaaacc tcgcgcctgg gtgctggatt ttaaaggcag catggacgcc
    18301 catgaagtga actcgctacg tgaagagata acggcggtac tcgcagcatt caaaccgcag
    18361 gatcaggttg tgctccgtct ggaaagccct ggtggcatgg tgcatggtta cgggttggcg
    18421 gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt taactgttac ggtagacaaa
    18481 gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg acaaaattgt ttccgcaccg
    18541 tttgctattg tgggttccat tggggtggtg gcgcaaatgc ccaactttaa ccgcttcctg
    18601 aaaagcaaag atattgatat cgaactgcac accgccgggc agtataagcg cacgctgacc
    18661 ttgctgggtg aaaataccga agaagggcgg gagaaattcc gcgaagagct gaacgaaacg
    18721 catcagttat ttaaagattt tgtgaagcgt atgcgtccgt ctctggatat tgaacaggtg
    18781 gcaacgggtg aacactggta cggacaacag gcggtagaga aaggcctggt tgatgaaatc
    18841 aacaccagtg atgaagttat tcttagcctg atggaaggcc gtgaagtggt taatgtacgc
    18901 tatatgcagc gtaaacgact cattgaccga ttcaccggca gcgcggcaga gagcgccgat
    18961 cgattgttgc tacgctggtg gcagcggggt caaaagccat tgatgtaaaa gacaaacgcg
    19021 aggctaagac ctcgcgtttt gctttaatca accagatgat atttttctga aagcacatgg
    19081 gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg ctgttggcaa tccttgttca
    19141 tctaaaaagt agtcgccggt aaataccagc acgccattac gctgcgtgac gccggtggct
    19201 tcaatccctg cgagcgtatc ctcatgctca cgaatgattt tgttagcctc tttcaacagc
    19261 gtttcgcggt cgatgggttg tgtctctttg ttcattattt actccttaaa caaggacatt
    19321 agtctacgcc aggcatggct tgcagacaaa tataccacgc tggtggcaag agcgccttac
    19381 tggcaacttt ggattttgca tgctaataaa gttgcgtatc ggattttatc aggtacagtg
    19441 tgacgctttc gtcaatctgg caatagattt gcttgacatt cgaccaaaat tccgtcgtgc
    19501 tatagcgcct gtaggccaag acctgttaac tcagtcacct gaattttcgt gaacagagtc
    19561 acgacaaggg ggtgatatcc gcagagagcg agtccatatc ggtaactcgt tgccagtgga
    19621 aggtttatca acgtgcgacg cattcctgga agaatcaaat taggtaaggt gaatatgggt
    19681 aaagctcttg tcatcgttga gtccccggca aaagccaaaa cgatcaacaa gtatctgggt
    19741 agtgactacg tggtgaaatc cagcgtcggt cacatccgcg atttgccgac cagtggctca
    19801 gctgccaaaa agagtgccga ctctacctcc accaagacgg ctaaaaagcc taaaaaggat
    19861 gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt ggcacaattg ggaggcgcat
    19921 tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac tgaaacaact ggctgaaaaa
    19981 gccgaccaca tctatctcgc aaccgacctt gaccgcgaag gggaagccat tgcatggcac
    20041 ctgcgggaag tgattggtgg tgatgatgcg cgctatagcc gagtggtgtt taacgaaatt
    20101 actaaaaacg cgatccgcca ggcatttaac aaaccgggtg agctgaatat tgatcgtgtt
    20161 aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg ggtatatggt ttcgccgctg
    20221 ctgtggaaaa agatcgctcg tggtctgtct gccggtcgtg tgcagtcggt ggcggtccgc
    20281 ctggtggtag agcgtgagcg tgaaattaaa gcgttcgtac cggaagagtt ctgggaagtc
    20341 gatgccagca cgactacgcc atctggtgaa gcgttggcgt tgcaggtgac tcatcagaat
    20401 gacaaaccgt tccgtccggt caacaaagaa caaactcagg ctgcggtaag tctgctggaa
    20461 aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga caaccagtaa acctggcgct
    20521 ccttttatta cctctacgct gcaacaagct gccagcaccc gtcttggttt tggcgtgaaa
    20581 aaaaccatga tgatggcgca gcgtttgtat gaagcaggct atatcactta catgcgtacc
    20641 gactccacta acctgagtca ggacgcggta aatatggttc gcggttatat cagcgataat
    20701 tttggtaaga aatatctgcc ggaaagtccg aatcagtacg ccagcaaaga aaactcacag
    20761 gaagcgcacg aagcgattcg tccttctgac gtcaatgtga tggcggaatc gctgaaggat
    20821 atggaagcag atgcgcagaa actgtaccag ctaatctggc gtcagttcgt tgcctgccag
    20881 atgaccccag cgaaatatga ctccacgacg ctgaccgttg gtgcgggcga tttccgcctg
    20941 aaagcacgcg gtcgtatttt gcgctttgat ggctggacaa aagtgatgcc tgcactgcgt
    21001 aaaggcgatg aagatcgtat cttaccggca gtcgataaag gcgatgctct gacgctcgtt
    21061 gaactaacac cagcccagca ctttaccaag ccgccagccc gtttcagtga agcatcgctg
    21121 gttaaagaac tggaaaaacg tggtatcggt cgtccgtcta cctatgcgtc gatcatttcg
    21181 accattcagg atcgtggcta tgtgcgagta gaaaatcgtc gtttctatgc ggaaaaaatg
    21241 ggcgaaatcg tcatcgatcg ccttgaagaa aatttccgcg agttaatgaa ctacgacttt
    21301 accgcgcaga tggaaaacag cctcgaccag gtggcaaatc acgaagcaga gtggaaagct
    21361 gtactggatc acttcttctc ggatttcacc cagcagttag ataaagctga aaaagatccg
    21421 gaagagggtg gtatgcgccc gaaccagatg gttctgacca gcattgactg cccgacttgt
    21481 ggtcgcaaaa tggggattcg cacagcgagc accggggtat tccttggctg ttctggctat
    21541 gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc tggtgccgga aaacgaagtg
    21601 ctgaacgtgc tggaaggcga agatgctgaa accaacgcgc tgcgcgcaaa acgtcgttgc
    21661 ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc cgaaacgtaa gttgcatgtc
    21721 tgtggtaata acccaacctg cgacggttac gagatcgaag agggcgaatt ccgcattaaa
    21781 ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct ctgaaatgca cctgaaaatg
    21841 gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt gtaaaaacac acgtaagatt
    21901 ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg tgccattacc tgagctgccg
    21961 tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg ctgccggtgt gttcctggct
    22021 gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga gctttatcgc
    22081 ttccgcgacc gtctgccgga aaaactgcgt tatctggccg atgcgccaca gcaggatccg
    22141 gaaggtaata agaccatggt tcgctttagc cgtaaaacca aacagcaata tgtctcttcg
    22201 gaaaaagacg gaaaggcgac tggctggtca gcattttatg ttgatggcaa atgggttgaa
    22261 ggaaaaaaat aacctttaat tctgtcaggt ttttataaac aaagggtcgc gaaagcggcc
    22321 cttttttatt gcatattatt ttttcttcac acctatacac taaggctata aatgatatag
    22381 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
    22441 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
    22501 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
    22561 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
    22621 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
    22681 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc
    22741 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
    22801 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
    22861 accagggccc gccgacacaa attgctgatg ccgtctctaa aggcaatgcc gatttcgcta
    22921 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
    22981 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
    23041 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
    23101 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgttttcacg gcaacggatg
    23161 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
    23221 cggtggatcc ggtcgccgat cccgaccttg tgcgcgttga tgctcacgat atcttcagcc
    23281 acagtacaac caaaattggt tttcgccgta gtactttttt gcgcagttat atgtatgatt
    23341 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
    23401 gctccaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
    23461 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta
    23521 cttatttcta taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt
    23581 aaagtagtgt ctaattaccc gcctcgcaaa ttccagtttt caaatatcta tttcaggtca
    23641 aaagattgaa ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt
    23701 ctttaggatt tgtctcatcg tatgattaat tacaccaatc attcaacgtc ttgctgataa
    23761 gtgatggcga ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgtgatc
    23821 ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg
    23881 ttgtcgcact gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga
    23941 gtcgtcacca ttatgccagc atagtgacaa tgaaccagaa acgaaacggg aatgttccgt
    24001 cgttattcca gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc
    24061 tgaagatgat cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt
    24121 aaagggcttt taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa
    24181 ccgttaaggg tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg
    24241 ttttctctgt cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg
    24301 accccgctgc ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt
    24361 tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag
    24421 aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc
    24481 cagtaaggac acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc
    24541 tgctaaatca ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa
    24601 cctgctgcgc tggcaggatg gtaactcagt taccgaagag gatatccacg cactggcggg
    24661 atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat
    24721 gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa
    24781 acgcctcggt ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga
    24841 ccactcggtg accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct
    24901 ggaaatggaa cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag
    24961 tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct
    25021 cggcaaagca gtgtggagcg aattgcagga cggtgaatgg attgcttatc cggatacact
    25081 cgttggtact gactcgcaca ccactatgat caacggcctt ggcgtgctgg ggtggggcgt
    25141 gggtgggatc gaagcagaag ccgccatgtt aggccagccg gtttccatgc ttatcccgga
    25201 cgtagtgggc tttaaactta ccggaaaatt acgtgaaggt attaccgcca cagacctggt
    25261 tctcactgtt acccaaatgc tgcgcaaaca tggcgtggtg gggaaattcg tcgaatttta
    25321 tggtgatggt ctggattcac taccgttggc ggatcgcgcc accattgcca atatgtcgcc
    25381 agaatatggt gccacctgtg gcttcttccc aatcgatgca gtaaccctcg attacatgcg
    25441 tttaagcggg cgcagcgaag atcaggtcga gttggtcgaa aaatatgcca aagcgcaggg
    25501 catgtggcgt aacccgggcg atgaaccgat ttttaccagt atgttagaac tggatatgaa
    25561 tgacgttgaa gcgagcctgg cagggccaaa acgcccacag gatcgcgttg cactgcccga
    25621 tgtaccaaaa gcatttgccg ccagtaacga actggaagtg aatgccacgc ataaagatcg
    25681 cctgccagtc gattatgtta tgaacggaca tcagtatcag ttacctgatg gcgctgtggt
    25741 cattgctgcg ataacctcgt gcaccaacac ctctaaccca agtgtgctga tggccgcagg
    25801 cttgctggcg aaaaaagccg taactcttgg cctcaagcgg caaccgtggg tcaaagcgtc
    25861 gctggcaccg ggttcgaaag tcgtttctga ttatctggca aaagcgaaac tgacacagta
    25921 tctcgacgaa ctggggttta accttgtggg atacggttgt accacctgta ttggtaactc
    25981 tgggccgctg cccgatccta tcgaaacggc aatcaaaaaa ggcgatttaa ccgtcggtgc
    26041 ggtgctgtcc ggcaaccgta actttgaagg ccgtatccat ccgctggtta aaactaactg
    26101 gctggcctcg ccgccgctgg tggttgccta tgcgctggcg ggaaatatga atatcaacct
    26161 ggcttctgag cctatcggcc atgatcgcaa aggcgatccg gtttatctga aagatatctg
    26221 gccatcggca caagaaattg cccgtgcggt agaacaagtc tccacagaaa tgttccgcaa
    26281 agagtacgca gaagtttttg aaggcacagc agagtggaag gaaattaacg tcacacgatc
    26341 cgatacctac ggttggcagg aggactcaac ctatattcgc ttatcgcctt tctttgatga
    26401 aatgcaggca acaccagcac cagtggaaga tattcacggt gcgcggatcc tcgcaatgct
    26461 gggggattca gtcaccactg accatatctc tccggcgggc agtattaagc ccgacagccc
    26521 agcgggtcga tatctacaag gtcggggtgt tgagcgaaaa gactttaact cctacggttc
    26581 gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc gccaatattc gcatccgtaa
    26641 tgaaatggtg cctggcgttg aaggggggat gacgcggcat ttacctgaca gcgacgtagt
    26701 ctctatttat gatgctgcga tgcgctataa gcaggagcaa acgccgctgg cggtgattgc
    26761 cgggaaagag tatggatcag gctcaagccg tgactgggcg gcaaaaggac ctcgtctgct
    26821 tggaattcgt gtggtgattg ccgaatcgtt tgaacgaatt caccgttcga atttaattgg
    26881 catgggcatc ctgccgctgg aatttccgca aggcgtgacg cgtaaaacgt tagggctaac
    26941 cggggaagag aagattgata ttggcgatct gcaaaatcta caacctggcg cgacggttcc
    27001 ggtgacgctt acgcgcgcgg atggtagcca ggaagtcgta ccctgccgtt gtcgtatcga
    27061 caccgcgacg gagttgacct actaccagaa cgacggcatt ttgcattatg tcattcgtaa
    27121 tatgttgaag taacaagtat ttgcttgccg gttattttgc ttccggcaag caaatgaatt
    27181 acacaatgca agagggttat ttgttcagca aatggcccat tttctcggct ttggtatcga
    27241 gataatgttc gttattgggg ttacgaccta caatcaatgg tacgcgttca acaatattaa
    27301 tccctgcttc ggtcagaatt tcgacttttt tcgggttatt ggttaacaag cggacttcat
    27361 tgacgccaag gagtttgaac atatcagcgc aaagagtgaa gtcgcgctca tcagcggcga
    27421 agcctaactg gtggttagcc tctacggtat cgtaaccttg atcctgcagt gcgtaagcgc
    27481 ggattttatt cagcagacca atgttacgac cttcctgacg gtgatacagc aaaataccac
    27541 ggccttcctc ggcaatttgc gtcaatgccg cttcgagctg gaagccacaa tcgcagcgca
    27601 agctgaacag ggcgtcaccg gtcagacatt cggaatggac gcgcgcaagt accggggtat
    27661 gcccggaaat atcgccatag actagcgcga catgatcgtg tccggttgcc agttcttcaa
    27721 atcccaccat caggaaatcg ccccatgggg ttggcagttt ggcttctgcc acacgtttaa
    27781 gctgcatgaa attctccaga taatgctggt tctgtattgg cttattttgc cataacgaga
    27841 aggggttcac ctaatcacga cgcgtcgatc gttcacggaa tggcacaaat ctgtcaattt
    27901 ttcttggaac tggcattttc agttatgatt gtgggactta tcaaaaagga gaggccatgc
    27961 gttcgattgc ccgacgtacc gcagtgggag ctgcactatt gtttgtcatg ccagtagccg
    28021 tatggatttc tggctggcgt tggcaacctg gagaacaaag ttggcgacta aaagcggctt
    28081 tttgggttac tgaaactgtc acccagccct ggggcgtcat tacacatttg attttgttcg
    28141 gctggtttct ctggtgtctg cgttttcgca ttaaggctgc ctttgtatta ttttccattc
    28201 tggcggcggc aatccttgtt ggacaaggcg ttaaatcctg gatcaaagac aaagtacagg
    28261 aaccacgacc ttttgttatc tggttggaaa aaacacatca tattccggtt gatgagttct
    28321 acactttaaa gcgagcagaa cgcggaaatc tagtaaaaga acagttggct gaagagaaaa
    28381 atattccaca atatttgcgt tcacactggc agaaagagac ggggtttgcc tttccttccg
    28441 gtcacacgat gtttgctgcc agttgggcac tgctggccgt tggtttgctg tggccgcgtc
    28501 ggcgaacgtt aaccattgct atcttgttgg tctgggcaac gggagtcatg ggaagccgcc
    28561 tgctactcgg gatgcattgg ccacgcgatc tggtggtagc tacgttgatt tcgtgggcgc
    28621 tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg gccattaaca ccacctgcgg
    28681 aagaaaatcg cgaaattgca caacgagaac aagaaagtta acgctggttg attttccgaa
    28741 tttaggcctt aaatcatcaa caatgcgcgt ggatgccatt tcgcagacgg cgcgaaaatg
    28801 gtactttaaa gggctattgc ggtaagttga ccataattta ttcgctctaa ccacataacg
    28861 ggaagtaatg tgaaatattt actcattttc ttactggtgt tagcgatctt cgtgatttcg
    28921 gtcacgttgg gtgcgcagaa cgatcaacag gtgacgttta attatctgtt agcgcaaggg
    28981 gagtaccgta tttccacatt gctggcggta ttgtttgctg cggggtttgc tatcggttgg
    29041 ttgatttgtg gcctgttctg gctgcgagtt cgtgtttccc tggcgcgcgc tgaacgtaaa
    29101 ataaagcgac tggaaaacca gctttcaccc gcgactgacg tggctgtagt gccgcactcg
    29161 tcagcggcga aggaataact ttctatgctg gagttgttgt ttctgctttt gcctgtagcc
    29221 gctgcctatg gctggtatat gggccgcaga agtgcgcaac aaaacaagca agatgaagcc
    29281 aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc tgcttagtaa tcaacaggat
    29341 aaagcggtag acctgtttct cgatatgctt aaagaggata caggcaccgt tgaagcccac
    29401 cttacgctcg gaaacctgtt ccgttcgcgt ggcgaagttg atcgcgctat tcgcatccat
    29461 cagaccctaa tggaaagcgc ctcgctgacc tatgaacagc gtctgttggc gattcaacaa
    29521 ctggggcgtg attacatggc cgccgggtta tacgaccgcg cggaagacat gttcaatcag
    29581 ctgaccgatg aaactgactt ccgcattggc gcgctgcaac agttgctaca aatctaccag
    29641 gctaccagtg agtggcagaa agcaattgat gttgccgaac gcctggtgaa gctgggtaaa
    29701 gataaacagc gcgtcgaaat tgcccatttc tactgtgagt tagccctgca gcatatggcc
    29761 agcgacgatc tcgatcgtgc catgaccttg ctaaaaaaag gggcggtggc agataaaaac
    29821 agcgcccgcg tatccatcat gatgggacgc gtgtttatgg cgaaaggaga atacgccaaa
    29881 gccgtcgaaa gtttgcaacg cgtcatatcc caggacagag aactggtcag cgaaacgctg
    29941 gaaatgttgc aaacctgcta tcagcagttg ggtaaaactg ccgaatgggc agaattcctg
    30001 caacgcgcgg tggaagagaa caccggtgcc gatgctgaat tgatgcttgc tgatatcatc
    30061 gaagcgcgcg acggtagtga ggctgcacag gtctatatta cacgccagct tcagcgtcat
    30121 ccgaccatgc gtgtattcca taagctaatg gattaccact taaatgaagc ggaagaaggg
    30181 cgcgccaaag agagtctgat ggtgctgcgt gacatggttg gcgagaaggt gcgtagtaag
    30241 cctcgttata gctgccagaa atgtggtttt accgcataca ccctctactg gcattgtccg
    30301 tcttgtcgtg cctggtcaac tattaaaccg attcgcggtc ttgatggcct gtaattttta
    30361 aaaaaatccg actttagtta caacatacta gttattgatg tcccattgtg ctccggcaat
    30421 gacggagcag aaaaagcctg ctaggggaac aatcgcaact gttaattttt tatttccacg
    30481 ggtagaatgc tcgccgttta cctgtttcgc gccacttccg gtgcccatca tcaagaaggt
    30541 ctggtcatga cgttaactgc ttcatcttct tcccgcgctg ttacgaattc tcctgtggtt
    30601 gttgcccttg attatcataa ttgtgatgac gcgctgtcct ttgtcgacaa gatcgaccca
    30661 cgcgattgtc gtctgaaggt cggtaaagag atgtttacat tgtttgggcc acagtttgtg
    30721 cgcgaacttc aacagcgtgg ttttgatatc tttcttgacc tgaaattcca cgatatcccc
    30781 aacactgcag cgcacgctgt cgctgctgca gctgacttag gcgtgtggat ggtgaatgtt
    30841 catgcctccg gtggggcgcg tatgatgacc gcagcgcgtg aggcactggt tccgtttggc
    30901 aaagatgcac cgcttttgat tgctgtgaca gtgttgacca gcatggaagc cagcgacctg
    30961 gccgatcttg gcgtgacact gtcacctgca gattatgcag aacgtctggc agcactgacg
    31021 caaaaatgtg gccttgatgg tgtggtgtgt tctgctcagg aagctgtgcg ctttaaacag
    31081 gtattcggtc aggagttcaa actggttaca ccgggcatcc gtccgcaggg gagtgaagct
    31141 ggtgaccagc gccgcattat gacgccagaa caggcgttgg cggctggtgt tgattatatg
    31201 gtgattggtc gcccggtaac gcaatcggta gatccagcgc agacactgaa agcgatcaac
    31261 gcctctttac agcggagtgc atgatgagtg attccaacag ccgtctggtc tactcaacgg
    31321 aaaccgggcg tattgatgag cccaaagcgg ccccagtacg ccctaaaggc gatggagcgg
    31381 tgcgtattca gcgtcagacc agtggacgta aaggtaaggg cgtttgcctg attaccggta
    31441 tcgatctcga tgatgccgaa ctgacaaaac ttgcagcgga actgaagaaa aaatgcggct
    31501 gcggcggagc agttaaagat ggagttattg aaatccaggg cgataagcgt gatttattaa
    31561 aatcactact tgaagcgaaa gggatgaagg taaaactcgc aggcggttaa cataaaaagc
    31621 cacggatata tccgtggctt tcgaatattt tactgtgtgt attattcagt tatttcgtac
    31681 tgaacagaa
//
LOCUS       DADWTH010000041        30047 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.041, whole genome shotgun sequence.
ACCESSION   DADWTH010000041 DADWTH010000000
VERSION     DADWTH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.041"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..108
                     /locus_tag="JNY02_001985"
     CDS             <1..108
                     /locus_tag="JNY02_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433530.1"
                     /translation="VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            338..721
                     /gene="secE"
                     /locus_tag="JNY02_001986"
     CDS             338..721
                     /gene="secE"
                     /locus_tag="JNY02_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBB9433531.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            723..1268
                     /gene="nusG"
                     /locus_tag="JNY02_001987"
     CDS             723..1268
                     /gene="nusG"
                     /locus_tag="JNY02_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBB9433532.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1427..1855
                     /gene="rplK"
                     /locus_tag="JNY02_001988"
     CDS             1427..1855
                     /gene="rplK"
                     /locus_tag="JNY02_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBB9433533.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1859..2563
                     /gene="rplA"
                     /locus_tag="JNY02_001989"
     CDS             1859..2563
                     /gene="rplA"
                     /locus_tag="JNY02_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBB9433534.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2976..3473
                     /gene="rplJ"
                     /locus_tag="JNY02_001990"
     CDS             2976..3473
                     /gene="rplJ"
                     /locus_tag="JNY02_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBB9433535.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3540..3905
                     /gene="rplL"
                     /locus_tag="JNY02_001991"
     CDS             3540..3905
                     /gene="rplL"
                     /locus_tag="JNY02_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBB9433536.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4225..8253
                     /gene="rpoB"
                     /locus_tag="JNY02_001992"
     CDS             4225..8253
                     /gene="rpoB"
                     /locus_tag="JNY02_001992"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBB9433537.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8330..12553
                     /gene="rpoC"
                     /locus_tag="JNY02_001993"
     CDS             8330..12553
                     /gene="rpoC"
                     /locus_tag="JNY02_001993"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBB9433538.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12795..13928)
                     /gene="thiH"
                     /locus_tag="JNY02_001994"
     CDS             complement(12795..13928)
                     /gene="thiH"
                     /locus_tag="JNY02_001994"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBB9433539.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13925..14695)
                     /gene="thiG"
                     /locus_tag="JNY02_001995"
     CDS             complement(13925..14695)
                     /gene="thiG"
                     /locus_tag="JNY02_001995"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBB9433540.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14697..14897)
                     /gene="thiS"
                     /locus_tag="JNY02_001996"
     CDS             complement(14697..14897)
                     /gene="thiS"
                     /locus_tag="JNY02_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBB9433541.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14881..15636)
                     /gene="thiF"
                     /locus_tag="JNY02_001997"
     CDS             complement(14881..15636)
                     /gene="thiF"
                     /locus_tag="JNY02_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBB9433542.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15629..16264)
                     /gene="thiE"
                     /locus_tag="JNY02_001998"
     CDS             complement(15629..16264)
                     /gene="thiE"
                     /locus_tag="JNY02_001998"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBB9433543.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16264..18159)
                     /gene="thiC"
                     /locus_tag="JNY02_001999"
     CDS             complement(16264..18159)
                     /gene="thiC"
                     /locus_tag="JNY02_001999"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBB9433544.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18240..18340)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18393..18869)
                     /gene="rsd"
                     /locus_tag="JNY02_002000"
     CDS             complement(18393..18869)
                     /gene="rsd"
                     /locus_tag="JNY02_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBB9433545.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18964..19737
                     /gene="nudC"
                     /locus_tag="JNY02_002001"
     CDS             18964..19737
                     /gene="nudC"
                     /locus_tag="JNY02_002001"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBB9433546.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19777..20841
                     /gene="hemE"
                     /locus_tag="JNY02_002002"
     CDS             19777..20841
                     /gene="hemE"
                     /locus_tag="JNY02_002002"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBB9433547.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20998..21669
                     /gene="nfi"
                     /locus_tag="JNY02_002003"
     CDS             20998..21669
                     /gene="nfi"
                     /locus_tag="JNY02_002003"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBB9433548.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21712..22302
                     /locus_tag="JNY02_002004"
     CDS             21712..22302
                     /locus_tag="JNY02_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBB9433549.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22489..22761
                     /gene="hupA"
                     /locus_tag="JNY02_002005"
     CDS             22489..22761
                     /gene="hupA"
                     /locus_tag="JNY02_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBB9433550.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22774..23469
                     /locus_tag="JNY02_002006"
     CDS             22774..23469
                     /locus_tag="JNY02_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBB9433551.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23471..23896)
                     /gene="zraP"
                     /locus_tag="JNY02_002007"
     CDS             complement(23471..23896)
                     /gene="zraP"
                     /locus_tag="JNY02_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBB9433552.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24134..25510
                     /gene="zraS"
                     /locus_tag="JNY02_002008"
     CDS             24134..25510
                     /gene="zraS"
                     /locus_tag="JNY02_002008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBB9433553.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25507..26832
                     /gene="zraR"
                     /locus_tag="JNY02_002009"
     CDS             25507..26832
                     /gene="zraR"
                     /locus_tag="JNY02_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBB9433554.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26829..28118)
                     /gene="purD"
                     /locus_tag="JNY02_002010"
     CDS             complement(26829..28118)
                     /gene="purD"
                     /locus_tag="JNY02_002010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBB9433555.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28130..29719)
                     /gene="purH"
                     /locus_tag="JNY02_002011"
     CDS             complement(28130..29719)
                     /gene="purH"
                     /locus_tag="JNY02_002011"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBB9433556.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7025 a         7838 c         8270 g         6914 t
ORIGIN      
        1 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
       61 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      121 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      181 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      241 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      301 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      361 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      421 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      481 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
      541 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
      601 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
      661 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
      721 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
      781 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
      841 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
      901 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
      961 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1021 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1081 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1141 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1201 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1261 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1321 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1381 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1441 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1501 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     1561 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     1621 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     1681 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     1741 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     1801 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     1861 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     1921 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     1981 cgtggacgta gctgttaacc tcggcatcga tgctcgtaaa tctgaccaga acgtacgtgg
     2041 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2101 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2161 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2221 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     2281 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2341 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2401 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2461 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     2521 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     2581 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     2641 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     2701 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     2761 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     2821 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     2881 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     2941 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3001 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3061 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3121 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3181 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3241 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3301 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3361 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3421 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3481 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     3541 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     3601 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     3661 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     3721 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     3781 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     3841 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     3901 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     3961 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4021 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4081 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4141 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4201 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4261 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4321 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4381 ttccgttctg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4441 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4501 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     4561 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     4621 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     4681 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     4741 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     4801 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     4861 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     4921 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     4981 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5041 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5101 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5161 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5221 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5281 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5341 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5401 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5461 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     5521 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     5581 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     5641 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     5701 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     5761 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     5821 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     5881 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     5941 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6001 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6061 aactacgtta tcgcccaggc gaactccaac ctggatgaag gaggccactt cgtagaagac
     6121 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6181 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6241 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     6301 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6361 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6421 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6481 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     6541 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     6601 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     6661 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     6721 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     6781 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     6841 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     6901 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     6961 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7021 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7081 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7141 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7201 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7261 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7321 cgccgaaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7381 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7441 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7501 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     7561 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     7621 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     7681 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     7741 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     7801 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     7861 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     7921 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     7981 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8041 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8101 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     8161 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8221 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8281 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8341 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8401 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8461 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     8521 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     8581 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     8641 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     8701 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     8761 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     8821 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     8881 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     8941 gctgaacgaa accaactccg aaactaagcg taaaaagctg accaagcgta tcaaactgct
     9001 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9061 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9121 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9181 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9241 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9301 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9361 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9421 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9481 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
     9541 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
     9601 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
     9661 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
     9721 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
     9781 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
     9841 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
     9901 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
     9961 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10021 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10081 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10141 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10201 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10261 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10321 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10381 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10441 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10501 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    10561 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    10621 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    10681 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    10741 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    10801 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    10861 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    10921 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    10981 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11041 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11101 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11161 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11221 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11281 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11341 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11401 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11461 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    11521 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    11581 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    11641 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    11701 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    11761 gttcgaagct cgtcgtccga aagaaccggc aatcctggct gaaatcagcg gtatcgtttc
    11821 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    11881 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    11941 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12001 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12061 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12121 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12181 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12241 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12301 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12361 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12421 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12481 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    12541 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    12601 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    12661 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    12721 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    12781 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    12841 cataccggct gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg
    12901 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    12961 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13021 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13081 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13141 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13201 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13261 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13321 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13381 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13441 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13501 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    13561 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    13621 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagttgtt agcgcaaaga
    13681 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    13741 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    13801 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    13861 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    13921 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    13981 tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg
    14041 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14101 cggcccccat ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa
    14161 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14221 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14281 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14341 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14401 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14461 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    14521 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    14581 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    14641 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cacaatacgt aacatctcaa
    14701 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    14761 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    14821 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    14881 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    14941 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15001 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15061 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15121 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15181 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15241 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15301 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15361 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15421 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15481 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    15541 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    15601 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    15661 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    15721 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    15781 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    15841 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    15901 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    15961 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16021 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16081 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16141 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16201 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16261 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16321 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16381 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16441 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16501 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    16561 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    16621 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    16681 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    16741 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    16801 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    16861 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    16921 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    16981 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17041 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17101 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17161 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17221 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17281 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17341 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17401 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17461 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    17521 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    17581 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    17641 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    17701 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    17761 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    17821 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    17881 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    17941 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18001 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18061 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18121 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18181 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18241 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18301 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18361 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18421 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18481 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    18541 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    18601 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    18661 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    18721 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    18781 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    18841 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    18901 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    18961 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19021 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19081 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19141 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19201 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19261 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc
    19321 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19381 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19441 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19501 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    19561 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    19621 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    19681 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    19741 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    19801 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    19861 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    19921 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    19981 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20041 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20101 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20161 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20221 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20281 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20341 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20401 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20461 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    20521 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    20581 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    20641 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    20701 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    20761 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    20821 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    20881 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    20941 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21001 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21061 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21121 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21181 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21241 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21301 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21361 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21421 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21481 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    21541 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    21601 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    21661 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    21721 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    21781 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    21841 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    21901 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    21961 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22021 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22081 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatgag cgatgaagag
    22141 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22201 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22261 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22321 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22381 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22441 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22501 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    22561 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    22621 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    22681 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    22741 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    22801 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    22861 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    22921 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    22981 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23041 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23101 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23161 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23221 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23281 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23341 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23401 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23461 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    23521 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    23581 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    23641 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    23701 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    23761 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    23821 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    23881 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    23941 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24001 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24061 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24121 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24181 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24241 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24301 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24361 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24421 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24481 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    24541 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    24601 tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    24661 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    24721 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    24781 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    24841 ggcgctaggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    24901 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    24961 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25021 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25081 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25141 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25201 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25261 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25321 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25381 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25441 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25501 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    25561 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    25621 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    25681 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    25741 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    25801 ggggcgctgg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    25861 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    25921 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    25981 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26041 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26101 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26161 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26221 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26281 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26341 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26401 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26461 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    26521 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    26581 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    26641 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    26701 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    26761 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    26821 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    26881 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    26941 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27001 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27061 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27121 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27181 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27241 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27301 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27361 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27421 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27481 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    27541 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    27601 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    27661 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    27721 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    27781 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    27841 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    27901 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    27961 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28021 cccggagcaa caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28081 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28141 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28201 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28261 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28321 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28381 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28441 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28501 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    28561 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    28621 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    28681 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    28741 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    28801 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    28861 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    28921 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    28981 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29041 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29101 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29161 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29221 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29281 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29341 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29401 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29461 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    29521 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    29581 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    29641 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    29701 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    29761 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    29821 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    29881 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    29941 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30001 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgcct
//
LOCUS       DADWTH010000042        29733 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.042, whole genome shotgun sequence.
ACCESSION   DADWTH010000042 DADWTH010000000
VERSION     DADWTH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.042"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            347..640
                     /locus_tag="JNY02_002012"
     CDS             347..640
                     /locus_tag="JNY02_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBB9433557.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JNY02_002013"
     CDS             712..1371
                     /locus_tag="JNY02_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBB9433558.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1463..1909
                     /locus_tag="JNY02_002014"
     CDS             1463..1909
                     /locus_tag="JNY02_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBB9433559.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNY02_002015"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNY02_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /locus_tag="JNY02_002016"
     CDS             3389..3808
                     /locus_tag="JNY02_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBB9433560.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5076
                     /gene="umuC"
                     /locus_tag="JNY02_002017"
     CDS             3808..5076
                     /gene="umuC"
                     /locus_tag="JNY02_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBB9433561.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSTSVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JNY02_002018"
     CDS             complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JNY02_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBB9433562.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JNY02_002019"
     CDS             complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JNY02_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBB9433563.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7560..8279
                     /gene="fadR"
                     /locus_tag="JNY02_002020"
     CDS             7560..8279
                     /gene="fadR"
                     /locus_tag="JNY02_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBB9433564.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8331..9863)
                     /locus_tag="JNY02_002021"
     CDS             complement(8331..9863)
                     /locus_tag="JNY02_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBB9433565.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARHYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10193..11491
                     /gene="dadA"
                     /locus_tag="JNY02_002022"
     CDS             10193..11491
                     /gene="dadA"
                     /locus_tag="JNY02_002022"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBB9433566.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11501..12571
                     /gene="dadX"
                     /locus_tag="JNY02_002023"
     CDS             11501..12571
                     /gene="dadX"
                     /locus_tag="JNY02_002023"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBB9433567.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12739..14165)
                     /locus_tag="JNY02_002024"
                     /pseudo
     CDS             complement(12739..14165)
                     /locus_tag="JNY02_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14775..16511)
                     /locus_tag="JNY02_002025"
     CDS             complement(14775..16511)
                     /locus_tag="JNY02_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBB9433568.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="JNY02_002026"
     CDS             complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="JNY02_002026"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBB9433569.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17620..18231
                     /gene="emtA"
                     /locus_tag="JNY02_002027"
     CDS             17620..18231
                     /gene="emtA"
                     /locus_tag="JNY02_002027"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBB9433570.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLRYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18233..18967)
                     /locus_tag="JNY02_002028"
     CDS             complement(18233..18967)
                     /locus_tag="JNY02_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBB9433571.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19168..19422
                     /locus_tag="JNY02_002029"
     CDS             19168..19422
                     /locus_tag="JNY02_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB9433572.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19472..20932)
                     /locus_tag="JNY02_002030"
     CDS             complement(19472..20932)
                     /locus_tag="JNY02_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBB9433573.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVPENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20869..21425)
                     /locus_tag="JNY02_002031"
                     /pseudo
     CDS             complement(20869..21425)
                     /locus_tag="JNY02_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
     gene            complement(21451..22305)
                     /locus_tag="JNY02_002032"
     CDS             complement(21451..22305)
                     /locus_tag="JNY02_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBB9433574.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22305..23114)
                     /locus_tag="JNY02_002033"
     CDS             complement(22305..23114)
                     /locus_tag="JNY02_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB9433575.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23124..23882)
                     /locus_tag="JNY02_002034"
     CDS             complement(23124..23882)
                     /locus_tag="JNY02_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB9433576.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APIIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23879..24859)
                     /locus_tag="JNY02_002035"
     CDS             complement(23879..24859)
                     /locus_tag="JNY02_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBB9433577.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24859..25881)
                     /locus_tag="JNY02_002036"
     CDS             complement(24859..25881)
                     /locus_tag="JNY02_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBB9433578.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWLPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26947..27387
                     /locus_tag="JNY02_002037"
     CDS             26947..27387
                     /locus_tag="JNY02_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433579.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(27466..29163)
                     /gene="treA"
                     /locus_tag="JNY02_002038"
     CDS             complement(27466..29163)
                     /gene="treA"
                     /locus_tag="JNY02_002038"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBB9433580.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLGTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(29483..>29733)
                     /locus_tag="JNY02_002039"
     CDS             complement(29483..>29733)
                     /locus_tag="JNY02_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBB9433581.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         7456 a         7225 c         7640 g         7412 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     4561 tcgtttggtg aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct
     4621 gcccgggcgg cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt
     4681 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcac gtcggtaaaa
     4741 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     4801 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     4861 agtcagggag tcgcgcagct caatttattc gatgacaatg caccgcgccc cgggagtgag
     4921 caattgatgg cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt
     4981 gccgggcagg ggatccaaca gcaatggcag atgaagcgag ctatgctttc accacgttat
     5041 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgtt
     5101 cccgcaggag cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg
     5161 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     5221 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     5281 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     5341 acaggtggca aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     5401 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     5461 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     5521 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     5581 tgccagagca gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa
     5641 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     5701 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     5761 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     5821 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     5881 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     5941 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     6001 cgacagaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     6061 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     6121 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     6181 catgttcaga tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa
     6241 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     6301 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga
     6361 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     6421 cgccaataat cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc
     6481 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     6541 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     6601 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     6661 tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac
     6721 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat
     6781 gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc
     6841 gatgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     6901 catcgaggaa cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt
     6961 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     7021 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacccctt
     7081 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     7141 cggggagtag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     7201 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     7261 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     7321 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgtttttg
     7381 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     7441 aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt
     7501 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     7561 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     7621 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     7681 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     7741 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa
     7801 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     7861 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc
     7921 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     7981 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     8041 acggtctgat ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg
     8101 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     8161 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     8221 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     8281 cccttccgtt taaagagcaa acccctcaaa cgaggggttt tttgttgttt ttacagattt
     8341 cccattcttg gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg
     8401 agcatcacat caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga
     8461 tccagcggtg cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa
     8521 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgataac
     8581 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     8641 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga
     8701 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     8761 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     8821 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     8881 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     8941 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag
     9001 aaggtcgcac agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     9061 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     9121 tccataaaat agagcaggtt ttcttgtggt tcggacggat agtggcgcgc ttcagcaacc
     9181 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga
     9241 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     9301 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     9361 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     9421 tcgacaatcg aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag
     9481 ttatgcccat agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc
     9541 atcaagtaag cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga
     9601 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     9661 ttaattggca tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca
     9721 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     9781 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     9841 ttaatagaat cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga
     9901 gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct
     9961 ttgcgatttc ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga
    10021 taaaaagggc gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat
    10081 gtgagtcagc tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa
    10141 gatgtaatta gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt
    10201 tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg
    10261 acatgaggtc accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa
    10321 tgccgggcaa atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa
    10381 agcgattaaa tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca
    10441 gtttcagttg aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga
    10501 aaacaaaggg cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg
    10561 cgccgaaacc aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac
    10621 cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc
    10681 gtatcagctg ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc
    10741 gcacaaactg acgggcggcc tgcagttacc caatgatgaa accggagact gccagctgtt
    10801 tacccagaat ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc
    10861 cgttgaccaa ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt
    10921 gattaaggcc gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg
    10981 cattgttgat attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca
    11041 ggaagatggt gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg
    11101 tttcgataac cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct
    11161 gttgcaaccg cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg
    11221 tcatgtcgag caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc
    11281 ggttgtcggg cgtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg
    11341 ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc
    11401 agcgatccca tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg
    11461 tccgggccat ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc
    11521 cagcctcgat ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca
    11581 cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg
    11641 gagcgcgctc ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt
    11701 acgtgagcgc ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga
    11761 tctggagatt tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa
    11821 agcactgcaa aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg
    11881 gatgaatcgg ttgggcttcc agcccgatcg cgtgcttacc gtctggcagc agttgcgggc
    11941 aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga
    12001 tggaatttcc ggcgcgatgg cgcgtattga gcaggcggcg gaggggctgg agtgtcggcg
    12061 ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg
    12121 gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac
    12181 cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc
    12241 gggcgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat
    12301 tgtcgccgca ggatacgccg acggttatcc gcgccatgcg cctaccggta ctcctgtttt
    12361 agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tggcggttga
    12421 tttaacgcct tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat
    12481 caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcgct
    12541 ggcgttacgc gtcccggttg tgacagtgta acttattgta agccggatcg gaggcagcgt
    12601 cttctggttg cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac
    12661 agagccattg cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga
    12721 actcgccgca caggcgttct acataaaacg cttacgcttc attattgact cggagtcgac
    12781 ttagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta
    12841 acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc
    12901 tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga
    12961 tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa
    13021 aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc
    13081 gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg
    13141 ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg
    13201 ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga
    13261 gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt
    13321 ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta
    13381 atcactctca ggttagtcga cagctcaggc agccagataa tacagttttc atagcacttc
    13441 agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag
    13501 tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg
    13561 cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg
    13621 ctaagattga gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct
    13681 gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct
    13741 tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct
    13801 tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc
    13861 cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag gcgagtcccc
    13921 aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca
    13981 atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc
    14041 tgcaaaattt gattcttgag ttccaccgac aggaagagcc gccctggcgg gcgtcgtacg
    14101 tctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc tttaaagcta
    14161 gccataatgc tacgtttcct tgaaataaat atcctctggc agcgccggag acgtgaatgt
    14221 ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc atcgttgact
    14281 ttttccctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg gcctaggtag
    14341 ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt tattaagcaa
    14401 aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga aacgcctatt
    14461 gtgtgggcta tgtcagtgcg gcaggttgcg tggggaatat gttcaggcga tgtcaggcag
    14521 gggcccctga tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa
    14581 aaagccctta cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa
    14641 tggtttgtca tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg
    14701 aaatgacaag tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag
    14761 atgctgcatt aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac
    14821 acttcattat cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca
    14881 ccgacaaccg gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta
    14941 tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg
    15001 ctggcttcga gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg
    15061 ctgaacagtt taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg
    15121 ccttcgcgca gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct
    15181 gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta
    15241 tcagcactca attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca
    15301 acgcgtgaca ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac
    15361 gagagtgatg ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag
    15421 aacagtcgcg cattctccag ccccgccatc atcgggaaca cggccaggat gattggcacc
    15481 gcgccgcgta atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa
    15541 gggagcaatc cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa
    15601 atgagcgccg gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc
    15661 aggaacatgg cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag
    15721 cggttgcgaa tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg
    15781 ctgccttcca gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat
    15841 ggatataatc cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg
    15901 ccaagaccga tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa
    15961 ctgacactgc tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc
    16021 attggatcat tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc
    16081 ttaccaccca gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt
    16141 aataagcctt caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca
    16201 gaggtgataa gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag
    16261 gaactggcct gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt
    16321 accatgtagg cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc
    16381 agcatgccga tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa
    16441 ctaagtaata tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt
    16501 gtggcatcca acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat
    16561 tagcggcaga gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac
    16621 aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg ttattcagaa tggcatgtgc
    16681 gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg
    16741 aatcgacagg cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata
    16801 atcattgggc gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat
    16861 tcccgcatga tagagctgca acagcatacg ttcgacgcgg aaagggtgct cgttaatatc
    16921 ttccagcacc agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat
    16981 cagcatcgca agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc
    17041 ttgccattca atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa
    17101 ggcgttcagt tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac
    17161 attgccttgc gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag
    17221 taacgggtca tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg
    17281 actggcaccg taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc
    17341 aagggaattg agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg
    17401 ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg
    17461 cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat
    17521 ggcaattcct tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg
    17581 tcacctcaac ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc
    17641 tttttgattg tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg
    17701 aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt
    17761 gcagcctggg gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt
    17821 aatccgaacg cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc
    17881 tccggacgtg atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg
    17941 aaaaatccag agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc
    18001 ccgctggcag gcattgaaga tccgaaggta ctgcgatatg cgctggtggt gtcatacgct
    18061 aacggggcag gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa
    18121 atcaacgatt tggatgctga tgagttcctc gatcacgtag cgcgaaatca tcctgcgccg
    18181 caggctccgc gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc
    18241 gcactttgtc cgctttttcc cgtgcttctc gctcgagaga gaaaataatc cgctgtaatt
    18301 gccgctccac cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg
    18361 tttcattctt gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt
    18421 gaaaaacacc ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta
    18481 attcggcagg ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca
    18541 agcggaaacg taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga
    18601 gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta
    18661 cggtaataaa tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact
    18721 cgactttcgc accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga
    18781 tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta
    18841 ttttgctgat cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca
    18901 aatcgcgtaa cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat
    18961 gactcacgcg gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac
    19021 aaaacttaat acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac
    19081 gcccagcatc gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga
    19141 ccgattcatc gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg
    19201 atagccggca ttatcgccaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg
    19261 acctgtattc tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc
    19321 attggcggct ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct
    19381 atcctggtgc tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat
    19441 agcgatacag atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga
    19501 aattgcgacc ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat
    19561 tgaccgacgc attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca
    19621 ccgcaaaacc ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct
    19681 cttccgacag cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg
    19741 tctgcgttgg aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca
    19801 gagcgtagct caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca
    19861 gaccgctgta atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt
    19921 gcgagaggat ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc
    19981 gcgtgaaggc accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgtg
    20041 ggttaacaat cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg
    20101 tcggaaagcg tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt
    20161 atttccccat cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc
    20221 tgccatcttt ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg
    20281 ccacgacatc gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac
    20341 gatcttcata gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt
    20401 tccagttaac ggcaaacgac agcagatcgt tttcaggcac atcggctgcc agttgtaatc
    20461 cggcaccgtc gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag
    20521 ccagcgagtt gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca
    20581 gggtaaaacg gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact
    20641 ctggccactg ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt
    20701 tttcaccatc ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac
    20761 cgagcttcac aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc
    20821 ctgcaatatc attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac
    20881 cgctgacttg caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg
    20941 cattgtcctg actacggctc catccctggc gatatcccag acttgcttct tcagggtggt
    21001 gatattaatg gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga
    21061 aacttcgact gcccccagat tgttggtcag aacccgcgcc agatcgaggt tgccgtcata
    21121 gggaacataa atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg
    21181 aacttttacc tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac
    21241 acttaatgcc tgagcgacat tctgtttatc caaggctttc atggtgggtt gatcgagaac
    21301 ggtggtcgtc gtcgatgata ccggactgga ccatacggtc agcgtgtcgc ctcctgagcc
    21361 gctacggcct gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt
    21421 ctcatagaat aaattccgtt taacaaagaa ttaaatagag gctgtgggtt gcagactgac
    21481 tttaatgtca gctggtgccg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca
    21541 gtcgaggtaa tttttgagtg tcgtcagatt aattccgccg gtcagagcca gcgtgcagtg
    21601 aggagccagc gacggcgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc
    21661 gagctgaagt acgtctggtt gtgcgcgtaa cgcggcgata gcctctttcg gcgtgtcggc
    21721 ttcaacaaca attttcttct cgggtgcgtg gcgacgtaac tgagcgattg cgcctgacca
    21781 gccctgattg tcatgaagaa aatggcggtg gttggcaaac aataatatgg tttccgcaca
    21841 tccggcacga tgaattagtc ctccggcagc cagaattgcc tgcgaagcca gtaagcgggt
    21901 gcccggaatt gcttttcggg tgcaggcgat attgccgtca ggataacgtt cacgaagcaa
    21961 tgccagcatt tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac
    22021 tgccttccat ccctgatgaa gtgctgcggc attaccctgc gcacggatta gccgttgacc
    22081 cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa
    22141 catcttacac gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa aaaacgctat
    22201 atagccaggc tggtgtccaa tatttaatgc tcgcgtggtc aggtcacctc cctggatatc
    22261 ttccagcagt aacgcatcga tttgtgcctg agaaaggaaa atcatcacag tgcctcctgc
    22321 ggtacgctat cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca
    22381 tcttgttgtt gataccagtg atataaccgc tcacgctcat cgtcgttaag cgcgccaaga
    22441 ctccagctga cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt
    22501 ccacggataa aatcaacgtg ggcataaatt cccatctgat acaggacgtt gagcgcaaag
    22561 atatagttgg gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg
    22621 aacgaggtgc tgaccagatg agttgtatag acgcgaagcc gtgcctgatt attcagtttg
    22681 ctcattgcat ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag
    22741 cgtggcaggt cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtcagcc
    22801 ttcagtgcgg cggcgcggcg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca
    22861 taaaccgtag tgagtttgtc agccagcgcc agcgatacgg tgcctggtcc gcagcccata
    22921 tcgaataagg tctgagcgcc ttgcaggtca attttagcga tcagttgttg caggtagctg
    22981 tccgttggcg aggcgcaatt ttccgccatt ttctccgccc gttgatccca gtgatcgggc
    23041 gttttttctg tgcggtgagc cagtttgagt tgctgcagat aaaggtctgc aaaatcaata
    23101 tcatcaatga gcatgattgt tccttagcgg ctaattgcag agaggtgatg atgaatttgg
    23161 tcggcagaaa cccgatacag ggccgccagt ttgttcgttg ttaactgttc ggtcggtaag
    23221 ccttgtgtta ctcgtccatc tggctctacc tggataatgc tgtcggcaat ggcgttggca
    23281 tggagcggat ggtgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc
    23341 gtgtccagaa gttggatctg atgaccgaaa tcgaggctgg aggcgggctc atccagtaac
    23401 aacaagcgcg gttgttggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg
    23461 ctaagcgtat tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc
    23521 gtggctttca ggcgttcttg cttaccggga acggaaaaag cgcctatgat gggggccagg
    23581 cccatcaaca ccatatccag cacactgaaa gcaaaaatac cgtcgtgcgc ctgcggcacc
    23641 caggcaatag cctgtgcgcg ctggcgatgg ctgagtgtct gcacagggag gctatccagt
    23701 aggatctcgc cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg
    23761 ccgcaaccat tcgcgccaag cagacaacaa attgttcctt gcgacagggt gaaggacacg
    23821 tcgcgtagca ctggatggcg ggagtaaccc agagacgcat gacgaacggc aagcaaagtc
    23881 actggcgacc tccgcgtaac aacaaaagca ggaaaaacgg tgcgccaaca aaagcggtca
    23941 gaatgccaag tgggatttct gttgttccaa tgctgcgcgc cagcgtatcg gtaagcagga
    24001 gcagaatagc gccagtacac attgccatag gcaacagctg ctgatggtta tgaccggtca
    24061 acaagcgggc aatgtgcgga acgaccaggc cgatccaacc aattatgccc gctattgcta
    24121 ccgtgctggc ggtgattagt gtggcgcaga caatcaatcc aaagcgcaga cgcgtcacgt
    24181 tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga
    24241 gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta
    24301 aggtcacggt ggacagtccc cccagcaacc agaaggtaat tgacggtaac tgggtgtaag
    24361 gatcggccag cgttttaatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc
    24421 ctaccagcac cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga
    24481 cgcccgcgac caccatcagc tcgccgcaaa atgcgtacag ctggatgtaa acaatgggta
    24541 atccccacag gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat
    24601 ccggtgaaac taagggatta cgcaacattc cctgataggt ggttccggct cctgccagcg
    24661 ctgcgcccag taacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt
    24721 gttcctgcac agacacattt tcttgacgaa gaattagcgc cagcagttgc tgcatatcga
    24781 gatgataagc cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac
    24841 atccgtttac gattctcatt agctcgccac caatttttga tagtcggcat cactgagtga
    24901 acaatgccag aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc
    24961 gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc
    25021 atcgagccag ccaaagggca agccacttaa aaataagatg cgttgttccg caacggcttt
    25081 cacgccctgc cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag
    25141 aataatatcc ggcagccagc gcaggagatt ttccatggaa acctgtgtca ggccgtggcg
    25201 atcggctatt tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga
    25261 accctgtaag cccgtttcca gccctcgggg accgcgcgca gcataaaagc ggaggttagc
    25321 ggcgggtgag gtggcgaatg cctgagcttc accaacgaag tggctggcga gattggcttg
    25381 ttctgcggcg cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga
    25441 ttgcgccagt tttccgttaa gcaataacca ggggatttgt gtctgttcgc taacctgccg
    25501 tgcctgggag atcaaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa
    25561 cgccataagc ccttccagcg atagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg
    25621 taactggcga atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga
    25681 aaaaccgacc attttttctg gcgcgacagc cagcaatagc aaatcggcgg gcgcgcctgc
    25741 gctgacgatg cgtgtaatgt tttgggcttc gggaagagat tgcgaagcaa aacgattaac
    25801 tgtttgtgaa aaggacggta gagactgcag gagcagggta gatgcaagtg cctgcgcaaa
    25861 cgtccgtctg gttatgggca tggtgaacat cctgaaaagc aaaatcgata tatactttca
    25921 tatatatcga taaaaatcat gttcaatatc aaagtttagc tattgaaaac atgaagatgt
    25981 gacgacttcc gcaagcctgt tgaccctctc tatcattaaa aatgtattta tcaggtggct
    26041 aatattattt aaatcattta aggctaaatc atttaaatat gaatgacatt attgtttttt
    26101 atcgtccacg gttacgcgac gttgaagtta cttgttttac aatgagcgat ttttgatatc
    26161 tcgccgagaa aattatcagc attctctcgt ttcagtgtcg tgaatatact gttaacggag
    26221 gtgaaaagaa tgctgggggt tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt
    26281 gctgatagta agcagcgtaa ccagaagtta ccgcaacaag actttgagga ttttaagaac
    26341 gacgttatta atgtcattaa caacaatgaa gatatttatt ttcttagaga gagtaattta
    26401 ctgaatgcgt ttattcgaac gtgcaaaaaa agagatgata acagttacgg aaatatgata
    26461 tttacgcttt attttgataa cgatatctat gggaacagaa ttatcaaagc atcgctgaac
    26521 ggcggagagt tgcagttatt aagcacaggg tgtagcaatg ataacattct ccattttatt
    26581 aatgcatttc tcattcgggt gaaatttccg ctacctggac gcataaaacc ggaactctcg
    26641 ccaaaagccg ggcactccga aggaattgac gcgttgcgct gctctgtaac ggcatttacc
    26701 gcaagccatt tatttcgcca gcgacaatcc gcagagaacc aaacgcatca ggaaacgact
    26761 gaacacaata aatactgtat tgatcaacat attatactgg cacctccagc acgcagtgag
    26821 gatacactgt atcatcagcg aatcttatca ctgcggagct ggttgaacga ctttctgagc
    26881 gaaataagtc ggttcccggt aacaacaaca gaatcctttt ttaattattg tttcgttgtt
    26941 tttggtgtga ttcagaaaat aaatatttta gacagtagcc agtcaggcgt taaaaatggg
    27001 gcggatagtg aggcggtttt ggctgcgctt attgaacatt tagaattgat caatccttct
    27061 ggcagattaa gccaaaatac ccgttcggca atgcttcagt tacgcgaaga ggaatggtct
    27121 gaattctttt tttggttgct aaattctctg gagtgtctgg attatgtgat tattaattta
    27181 actccagaaa gcaaaaaaac gttgatgagt gaacaccgta acaatattca ggtagcaatt
    27241 gatgctttat atagccaacg tcggcgtaaa tcgccgggtg atgaaagtga aacacttacc
    27301 cgtagaaatg acgctatctt cggcaaccac gtatggcaaa catttgcgca atacttcccg
    27361 ccaggacttg aaaaaccgtc tgtgtagaaa aaaccataga tatggtgtta tctgccgaca
    27421 acgccatccg gagaagccga atgacgttgt cggagtaagc gctggttaag gtgtgggttg
    27481 tgcctctttg gttgagggtt gcgtcgttgc tgacttaacg gtcggacgcg tcgccggaac
    27541 attgtcacac ggttgctctt tcgggcagat caaatccagc attttcagcg tcacgccatt
    27601 ggtccagcca aagccatcct gtaatggata ttcgccaccg ccgccccccg ttccggtggt
    27661 gctgacatca tatttttcca ccagcttttt ctcccggtca taggtgtgct gaacattggt
    27721 caggaagtgc cagctaatgt ccatcgccac ctctttttgc ccgtagtttt gtaatccttc
    27781 tgtcgcgacc cactgtaacg gtgcccagcc atttggcgca tcccattgtt gcccactttt
    27841 caccgacgtg gtgttcaggc cgccgggttg cagcagatgt gttttcgtcg ccgtcgccat
    27901 tttgttggcg cgatctttcg ctgccgcatt gacgtacagc gggaacaggg cggccgcggt
    27961 taactgattg cgcactttat gacttttcag gtcgtaatcg gcataccagc cttgttgatc
    28021 gttccacagg tatttttcga tccctttttg acgggcattc gccagcgttt cgtactggtt
    28081 tgccatcgcg ttatctcctg cagctttgct ggcgcgggcg aggatttttt ccattttaaa
    28141 catcaggctg ttcagatcga ccggtacgat gctggtggtg cctaaggtat ttaactgctg
    28201 cgggttgtcc atccagcgcg agctgaaatc ccagccagac gcagcggcag agcgcaggtc
    28261 gcggtaaatt tcagtggcag gtcgattcgg attgcttttg gcggtggcaa tatcttccac
    28321 ccatgactct ggtcgtggcg tatcgcgatc gtcccagtag cggttgagaa gggtaccatc
    28381 ctgaagtttt acaacgcgtt tttcctgttg tccggcttgc aggttttcaa caccgtccat
    28441 ccagtaagca tattcttttt gcatttgcgg caggtattgc ttcaacgcgg catcgccttc
    28501 atgctgcgcc agtaactcta ccatcagggc aaagaagggc ggttgcgagc ggcttaaata
    28561 gtaactgcgg ttgccgttgg gaatatgacc gtaagtgtct atttcatgag caaaattggc
    28621 caccatatcc gcgactttat cccagtgacc gctttcggca agtcctaaca tggtgaagta
    28681 actgtcccag taatatacct cgcgaaaacg tccgcccggc acgacataag gttccggcag
    28741 cggtaacaga gaatcccatt tttcggtgtt ttcggtagaa cgcgttaata ccggccaaag
    28801 tccgtcaata tgttcgcgca gtgactgccc ctctggcgga acatatttct cgccttcttt
    28861 cggcagggtg aaattgacgt taacgaaatg gcgcagatca aatccgctct ggttttgctg
    28921 catccgataa tcagcaagga tcatcagcgg atcgctgttc ggcacggcat cggcaaaggt
    28981 tttttggtcc ggaaaaagtt tggcgttttg cacatcatta aacagcggcc ctaataaaat
    29041 atcaggcggc tgtggtgtta ccggtgtttc ttctgcctgc accgatagcg cagcgaaaca
    29101 caaaaagata caggctggaa ttaacgccat tttttgcggg cgagaaggtg cgggggattt
    29161 catcaatcat tctcctttgg cgaaaccgaa taaaacggta ttcaagtatc agaaaacctt
    29221 agttcaggat cgcactagct gcatgatcga aatctcattc tgcgaacggt caggcatttt
    29281 agaaaaggga attaaaggga taaataacgg cgaaatagag tgatcaaata acgtctgttt
    29341 attagccagc cagcgacatt tggttcgcga ttagcgaaga aaaatcatca taaattggta
    29401 aaaaatataa caggtagtta aaaaacatca gtgctgggta aattgccgga tgacatcaga
    29461 acgatgccat ccgaacggtg gcttaaccct gacggttgaa acgttgcgtt ttaacgtcca
    29521 gcgttagcgt ttcttctggt tgtatcgcat acagtttctc accctgctgg caaatccagc
    29581 caatccccag ttcacgggcg attagggcgc tgtgggatac cggacttccg gcgctaaggc
    29641 agataccttt tacaaccgcc ggatccagtt gcagtactgt ggaaggataa atgttctccg
    29701 ccagtagaat agttggcgag ttaaactgcg gga
//
LOCUS       DADWTH010000043        29417 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.043, whole genome shotgun sequence.
ACCESSION   DADWTH010000043 DADWTH010000000
VERSION     DADWTH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.043"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..109)
                     /locus_tag="JNY02_002040"
     CDS             complement(<1..109)
                     /locus_tag="JNY02_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HBB9433582.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(179..1168)
                     /gene="araF"
                     /locus_tag="JNY02_002041"
     CDS             complement(179..1168)
                     /gene="araF"
                     /locus_tag="JNY02_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBB9433583.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAH
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1964..2467
                     /locus_tag="JNY02_002042"
     CDS             1964..2467
                     /locus_tag="JNY02_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBB9433584.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2547..2798)
                     /locus_tag="JNY02_002043"
     CDS             complement(2547..2798)
                     /locus_tag="JNY02_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBB9433585.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2913..2999)
                     /gene="azuC"
                     /locus_tag="JNY02_002044"
     CDS             complement(2913..2999)
                     /gene="azuC"
                     /locus_tag="JNY02_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBB9433586.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3262..3585
                     /locus_tag="JNY02_002045"
     CDS             3262..3585
                     /locus_tag="JNY02_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBB9433587.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3756..4253
                     /gene="ftnA"
                     /locus_tag="JNY02_002046"
     CDS             3756..4253
                     /gene="ftnA"
                     /locus_tag="JNY02_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBB9433588.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4290..4529)
                     /locus_tag="JNY02_002047"
     CDS             complement(4290..4529)
                     /locus_tag="JNY02_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBB9433589.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4719..5930
                     /gene="tyrP"
                     /locus_tag="JNY02_002048"
     CDS             4719..5930
                     /gene="tyrP"
                     /locus_tag="JNY02_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBB9433590.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5992..6657)
                     /gene="yecA"
                     /locus_tag="JNY02_002049"
     CDS             complement(5992..6657)
                     /gene="yecA"
                     /locus_tag="JNY02_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBB9433591.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6853..6939)
                     /locus_tag="JNY02_002050"
     tRNA            complement(6853..6939)
                     /locus_tag="JNY02_002050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6903..6905),aa:Leu,seq:taa)
     gene            complement(6952..7025)
                     /locus_tag="JNY02_002051"
     tRNA            complement(6952..7025)
                     /locus_tag="JNY02_002051"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6991..6993),aa:Cys,seq:gca)
     gene            complement(7307..7855)
                     /gene="pgsA"
                     /locus_tag="JNY02_002052"
     CDS             complement(7307..7855)
                     /gene="pgsA"
                     /locus_tag="JNY02_002052"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBB9433592.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7912..9744)
                     /gene="uvrC"
                     /locus_tag="JNY02_002053"
     CDS             complement(7912..9744)
                     /gene="uvrC"
                     /locus_tag="JNY02_002053"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBB9433593.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9741..10397)
                     /gene="uvrY"
                     /locus_tag="JNY02_002054"
     CDS             complement(9741..10397)
                     /gene="uvrY"
                     /locus_tag="JNY02_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBB9433594.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10693..10869
                     /locus_tag="JNY02_002055"
     CDS             10693..10869
                     /locus_tag="JNY02_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433595.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10856..11080
                     /locus_tag="JNY02_002056"
     CDS             10856..11080
                     /locus_tag="JNY02_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBB9433596.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11113..11733)
                     /gene="sdiA"
                     /locus_tag="JNY02_002057"
                     /pseudo
     CDS             complement(11113..11733)
                     /gene="sdiA"
                     /locus_tag="JNY02_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11963..12715)
                     /gene="yecC"
                     /locus_tag="JNY02_002058"
     CDS             complement(11963..12715)
                     /gene="yecC"
                     /locus_tag="JNY02_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBB9433597.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12712..13380)
                     /gene="tcyL"
                     /locus_tag="JNY02_002059"
     CDS             complement(12712..13380)
                     /gene="tcyL"
                     /locus_tag="JNY02_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBB9433598.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13395..14381)
                     /gene="dcyD"
                     /locus_tag="JNY02_002060"
     CDS             complement(13395..14381)
                     /gene="dcyD"
                     /locus_tag="JNY02_002060"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBB9433599.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14486..15286)
                     /gene="tcyJ"
                     /locus_tag="JNY02_002061"
     CDS             complement(14486..15286)
                     /gene="tcyJ"
                     /locus_tag="JNY02_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB9433600.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15374..15925)
                     /gene="fliZ"
                     /locus_tag="JNY02_002062"
     CDS             complement(15374..15925)
                     /gene="fliZ"
                     /locus_tag="JNY02_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBB9433601.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15971..16690)
                     /gene="fliA"
                     /locus_tag="JNY02_002063"
     CDS             complement(15971..16690)
                     /gene="fliA"
                     /locus_tag="JNY02_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBB9433602.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDAPQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16855..18507)
                     /gene="fliC"
                     /locus_tag="JNY02_002064"
     CDS             complement(16855..18507)
                     /gene="fliC"
                     /locus_tag="JNY02_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBB9433603.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18773..20179
                     /gene="fliD"
                     /locus_tag="JNY02_002065"
     CDS             18773..20179
                     /gene="fliD"
                     /locus_tag="JNY02_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBB9433604.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20204..20614
                     /gene="fliS"
                     /locus_tag="JNY02_002066"
     CDS             20204..20614
                     /gene="fliS"
                     /locus_tag="JNY02_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBB9433605.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20614..20979
                     /gene="fliT"
                     /locus_tag="JNY02_002067"
     CDS             20614..20979
                     /gene="fliT"
                     /locus_tag="JNY02_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBB9433606.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21057..22544
                     /gene="amyA"
                     /locus_tag="JNY02_002068"
     CDS             21057..22544
                     /gene="amyA"
                     /locus_tag="JNY02_002068"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBB9433607.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22578..22991)
                     /locus_tag="JNY02_002069"
     CDS             complement(22578..22991)
                     /locus_tag="JNY02_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB9433608.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23178..24383
                     /gene="yedE"
                     /locus_tag="JNY02_002070"
     CDS             23178..24383
                     /gene="yedE"
                     /locus_tag="JNY02_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBB9433609.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24380..24613
                     /gene="yedF"
                     /locus_tag="JNY02_002071"
     CDS             24380..24613
                     /gene="yedF"
                     /locus_tag="JNY02_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBB9433610.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24720..25390
                     /locus_tag="JNY02_002072"
                     /pseudo
     CDS             24720..25390
                     /locus_tag="JNY02_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25430..26638)
                     /locus_tag="JNY02_002073"
                     /pseudo
     CDS             complement(25430..26638)
                     /locus_tag="JNY02_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            26706..27321
                     /gene="tnpA"
                     /locus_tag="JNY02_002074"
                     /pseudo
     CDS             26706..27321
                     /gene="tnpA"
                     /locus_tag="JNY02_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            27649..28731
                     /locus_tag="JNY02_002075"
     CDS             27649..28731
                     /locus_tag="JNY02_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBB9433611.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(29220..>29417)
                     /locus_tag="JNY02_002076"
     CDS             complement(29220..>29417)
                     /locus_tag="JNY02_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9433612.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT         7415 a         7407 c         6888 g         7707 t
ORIGIN      
        1 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
       61 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
      121 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
      181 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagta
      241 ccacgtctgt aacttcggta aattttggcg tttcaacgtc ttttgctacc cagttgtaaa
      301 gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc
      361 cggttgcctg tgctttagac agttcgctta ctgcatccac accgttaata ccaataccga
      421 tgatatcggc tgctttaaag ccctgacctt cggttgcacg tacgccgcca agtacggtgc
      481 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
      541 tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga taaatttgtt
      601 tttccgggaa tcctgcagct ttcagtgcgt ccatagaacc cgttgtacgg cggcgggcgg
      661 tatccagttc gttggcggta atcgccatca ctgcgctttc tttgacatcc cagccacgtt
      721 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttagtc gccgccatca
      781 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccactg
      841 caatgacttt catatcgtag ccatgcgctt tcgcgacgat ggcagagccg agtttcgggt
      901 ccggagtaca aataacgaaa ccttttgcgc cactggctgc caggctgtcg atcgcgttca
      961 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
     1021 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagaa
     1081 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
     1141 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta
     1201 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttatcta
     1261 aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca taggagagca
     1321 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa agacataagg
     1381 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
     1441 tggaaatcct tgttgtcttc ggtacccatg cgggatgtct tctttttaac cagtcaatag
     1501 gccgcattac ctggcgttga gtttttgaaa tggtgtaata accacaactc aaagatgtgg
     1561 aaaatgcgcg tcattcattt cgccattaat tatcactgtg ctcattaatt aacagaacac
     1621 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat cacttcatca
     1681 tgaattcaaa ttcatcgatt aatatcaaca agatacaaaa gcactatcat taaaattcat
     1741 tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat gatttaagca
     1801 ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa ttgactccac
     1861 gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat ggtgttttga
     1921 tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa ccgctggaat
     1981 gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc tctaccttca
     2041 cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc ttcgcgccca
     2101 ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc
     2161 tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact
     2221 gttccaaaaa acgatggaag aatacgagca acgttcaagc acgctggcgc agttagccga
     2281 tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga
     2341 acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact
     2401 tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt cacaccagct
     2461 gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag aaaccgctgc
     2521 ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt aaacttgagt
     2581 tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc ggcagcggga
     2641 tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac ttcaacgggc
     2701 gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc taccacatca
     2761 gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg atagcatttt
     2821 gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac cgtcatcagg
     2881 acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc tggcggtacg
     2941 tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg cagtttcatc
     3001 gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata atatgacaac
     3061 catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt aatgttataa
     3121 aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca agaaatagcg
     3181 cctgccaggc atcttttccg gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc
     3241 tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt aataacgctg
     3301 gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt
     3361 gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg ttatgtcagt
     3421 cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat cccctttggt
     3481 cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac ggaattcact
     3541 ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt ttaatcagcg
     3601 aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat tcgtattttt
     3661 aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt atagtgacgc
     3721 cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa atgattgaaa
     3781 aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag caaatgagcg
     3841 cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg
     3901 aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc aatttaccgc
     3961 gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa ttattccagg
     4021 aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct catgctgcaa
     4081 tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct gagcagcatg
     4141 aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc aaaagcggcg
     4201 aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac taatgctctc
     4261 cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact ctgatcggtc
     4321 gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc taccagctct
     4381 cccgctgtca tcccttctgc cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct
     4441 ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat catcatattt
     4501 aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg
     4561 attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt gtacatttat
     4621 atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc
     4681 agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa
     4741 gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg
     4801 cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt
     4861 gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg
     4921 gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga
     4981 tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa ttgttggcct
     5041 ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca
     5101 cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt
     5161 ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata
     5221 ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg tctgcaatcc
     5281 cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg
     5341 atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc cccctggtgg
     5401 catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc tttatgggat
     5461 tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa atggtgccct
     5521 ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc acgtcatttc
     5581 tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt tcaaataccg
     5641 ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt
     5701 tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg
     5761 cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct caggcgggtt
     5821 accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga
     5881 ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga tcagatagcc
     5941 tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct cttaatgcag
     6001 gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac gccccggttt
     6061 ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtatgc
     6121 atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga
     6181 cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc
     6241 tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc
     6301 aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa
     6361 cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa
     6421 agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca catagtcagc
     6481 cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag aactcaacac
     6541 cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt
     6601 ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg
     6661 cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga
     6721 tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg acaagttgca
     6781 ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt gattttattc
     6841 ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga
     6901 ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc
     6961 gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac
     7021 gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact
     7081 ggagcgggaa acgagactcg aactcgagac cccgaccttg gcaaggtcat gctctaccaa
     7141 ctgagctatt cccgtattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg
     7201 caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat
     7261 atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca
     7321 aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca
     7381 caaagaaaag tgcaataccg gcgtactcaa cccaaatgtt cggacgccac agcagccagg
     7441 ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg gccacgctac
     7501 tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga
     7561 tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt accagcacca
     7621 tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca aaccgggtac
     7681 tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga
     7741 aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga
     7801 atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga
     7861 cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt
     7921 caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc
     7981 ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg
     8041 acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg
     8101 ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac
     8161 atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct caaagaacag
     8221 cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt
     8281 tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt
     8341 gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt taccataacg
     8401 ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt
     8461 atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac
     8521 ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa
     8581 tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt
     8641 gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg ccctatcgcc
     8701 gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag
     8761 cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg
     8821 gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca gttccgtacc
     8881 gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa
     8941 tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc
     9001 ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat
     9061 acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc taatgagttg
     9121 cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc
     9181 gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctggta
     9241 ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa
     9301 aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa
     9361 atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc
     9421 actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg
     9481 atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt
     9541 tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct
     9601 gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt
     9661 accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc
     9721 ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg
     9781 cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga
     9841 tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc
     9901 ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat
     9961 gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatatcagaa
    10021 gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc
    10081 gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg taaagggttt
    10141 tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc gattttacgc
    10201 gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca
    10261 tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac gacttttata
    10321 ccctttatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca
    10381 agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt
    10441 cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga
    10501 tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg
    10561 gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat tgactttatt
    10621 cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg
    10681 caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt ttatagcact
    10741 gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc
    10801 gccgccttaa catttttcac cgcaaatttt cattgcaaca tgacgaggaa aataaatgag
    10861 tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct
    10921 gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat
    10981 gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc
    11041 caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgagagcg
    11101 tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct tcccttccgc
    11161 cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat
    11221 ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa
    11281 ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa
    11341 gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc
    11401 gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc acattaaatg
    11461 gtcctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat tttttgcctg
    11521 ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa
    11581 tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag
    11641 ctctatttca tggtagacct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt
    11701 gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga
    11761 gtatcattta taatgataat tcaaactatc acataaataa gatttatata taatttatat
    11821 tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat taatgagtta
    11881 tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg acaggtgaat
    11941 cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac tggcgggtgc
    12001 gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccct
    12061 ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa
    12121 tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact tcgcccacca
    12181 gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga cgcattgcca
    12241 gtgcacgcgc aatcgcaaca cgctgctgtt gacctccaga caaacgacgt ggatagctgg
    12301 tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt
    12361 ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc accgtacgat
    12421 gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca
    12481 gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg
    12541 ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt ttgccggaac
    12601 cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca
    12661 gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca ctcattttgg
    12721 ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataacacagt
    12781 cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg
    12841 cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga
    12901 cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc
    12961 ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac tggcagcggc
    13021 ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc
    13081 ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt cgataccaaa
    13141 ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat
    13201 agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc
    13261 gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt gcagggtata
    13321 cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac tttcttgcat
    13381 gagcgttccc ggagttaaac gtggggatga taggcgaaca gcgcaggcgc gccgccggta
    13441 tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc gtcaatcagc
    13501 cccgccatcg cttttccggt atacacagga tcaagcagaa taccttccag acgcgccagc
    13561 aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca
    13621 tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt
    13681 aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac gccaatcagt
    13741 tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt tccggcactg
    13801 cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg cgcgatttcc
    13861 agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac cggaatgaca
    13921 tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct cttccagttg ggcattggga
    13981 tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa caaacgattg
    14041 ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacgcag
    14101 tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga ctggatcgcg
    14161 ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag aaattccagc
    14221 ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt gatgaaaatt
    14281 tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg cgcgccgata
    14341 aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc cgatgtgtgt
    14401 tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa
    14461 gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg
    14521 aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc accgctttca
    14581 gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac
    14641 cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacgatcaa
    14701 cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg tcatcatcat
    14761 aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag ttggtgccca
    14821 gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg ccttcgttac
    14881 cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt
    14941 tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt ttagagtcca
    15001 gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt
    15061 ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca tccccctgaa
    15121 aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgcgc tctttgactt
    15181 tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgccacgc
    15241 ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc accccgaatg
    15301 ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac
    15361 atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc
    15421 cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg taattgttgg
    15481 tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat
    15541 cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga acgacatatt
    15601 cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta aagccgataa
    15661 tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg
    15721 cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc aggcgggaaa
    15781 tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac
    15841 ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc
    15901 gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca
    15961 tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc tggctgtgta
    16021 actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg agattcagct
    16081 cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatggctt
    16141 ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt tctcgctgat
    16201 gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca tcgtaggaga
    16261 agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata tcgatcccta
    16321 aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta
    16381 ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc
    16441 gaagttcatc cagcatagcg ccacggatac gctgcactgc gtaagttgta aatgccgttc
    16501 cttgtggggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc gcctgtagca
    16561 gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca
    16621 ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca gcggtataga
    16681 gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg
    16741 cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttacgcaaa
    16801 aagccgaatg taaaaaaccc cgtcggtggc ggggtttgag aggcattcag acgattaacc
    16861 ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca cggagttacc
    16921 ggcctgcagg ataatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    16981 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    17041 ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga tggagctgat
    17101 ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt cagcatctgt
    17161 cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag tcactgaacc
    17221 atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt tgttatcaac
    17281 ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc tataacctgc
    17341 agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag ctgcctgatc
    17401 aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat taccggtaaa
    17461 ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg ctgcaagtgt
    17521 ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc gcccagttgt
    17581 atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc tgatctgaac
    17641 atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa ctcccggggt
    17701 caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt atgacttact
    17761 tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt tattaatggt
    17821 tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt tagcagcatc
    17881 gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag ctaccaagtc
    17941 agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa acccattcag
    18001 ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc cgtcattcgc
    18061 accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt tgaactgggt
    18121 ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct gaatggagtc
    18181 cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac ggatacgctg
    18241 taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag aaataccgtc
    18301 gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac ggttagcaat
    18361 cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag acagacgctc
    18421 gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga tcagcgagag
    18481 gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag tcgttgatta
    18541 cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt ctgtcgtaaa
    18601 acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga aatactcttt
    18661 tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt gcgcaattca
    18721 ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc gcatggcaag
    18781 tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg atagcctcac
    18841 cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt ttaccgctaa
    18901 acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg ccaatactgc
    18961 attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca ccgcgttcag
    19021 tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca cccatctggc
    19081 gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg cgatcgccac
    19141 cagcgacagc aaactcacca ttcaacaagg cggcgacaaa gatccgatta ccattgatat
    19201 cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg caaaagcggg
    19261 cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag tcacatcaaa
    19321 tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg cgctacaaag
    19381 ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg tcgccgccca
    19441 gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca ctatcagcga
    19501 cgcgctggaa aacatcaccc tgaacctgaa cgatgtcacc acgggcaacc agacgctaac
    19561 catcactcag gacacctcca aagcgcaaac ggcgattaaa gactgggtga atgcctataa
    19621 ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg cgggagctga
    19681 tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga cgattcagac
    19741 gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa cgctggcgca
    19801 gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg acaaactcac
    19861 cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg atggtaaaaa
    19921 aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga caacgggcat
    19981 tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta aagactacaa
    20041 cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat ttacccaact
    20101 ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc agttcgaaaa
    20161 caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg cggcaaaagg
    20221 cacccaggcc tatgcgcaaa ttggcgtcga aagcaccgta atgagcgcca gccaacagca
    20281 gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc gactgtttat
    20341 gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca acatcattga
    20401 gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc aaaacttaat
    20461 tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca acgatgtctc
    20521 cgcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga aagagtcttt
    20581 actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca tttgtatttc
    20641 gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac ggaagaacaa
    20701 tgggatgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga gatcgcacac
    20761 ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc gatgctgcac
    20821 ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat ggatgaactg
    20881 gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta tggcgatcag
    20941 ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag ccgatggctc
    21001 gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt cgaagaatgc
    21061 gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt aagctctggc
    21121 ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg gtctggttgc
    21181 cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc tatgatttat
    21241 ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt gataaagcac
    21301 aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg ctggacgtgg
    21361 tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag cgtgtaaatg
    21421 ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg acgcgttaca
    21481 ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa tgttttagcg
    21541 gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt aacgactata
    21601 ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat tatctgatgg
    21661 gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac tgggcgcgct
    21721 gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa catattccgg
    21781 catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag ccgctgttta
    21841 ttgtggctga atactggtcg catgaagtcg ataaactgca aacgtatatt gatcaggtgg
    21901 aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca
    21961 tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa gccgatcctt
    22021 ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc ctcgaagcac
    22081 cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg gaaaatggcg
    22141 ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc ggtggtgacg
    22201 ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta attctcgccc
    22261 gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac tgcattgcct
    22321 ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg aacggagatg
    22381 atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg cgtgattttc
    22441 tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc ttcttttgca
    22501 acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat ccccggcggc
    22561 aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc cagcgcggcg
    22621 gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta ctcaatggtg
    22681 ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt gagatcatca
    22741 gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg gcaatccaag
    22801 gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac cagcgcacgt
    22861 tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt tttcacgaca
    22921 ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc tgcaattgcg
    22981 agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt aaagctctga
    23041 tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt ttgtgaatta
    23101 ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca aaccttctga
    23161 gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg attaaattct
    23221 gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat tttggcatta
    23281 ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag ctcctgcagc
    23341 tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg gaaggatcgc
    23401 cattaacccg catcgacggc atgatgatcc tcggtatgtt tggcggctgc tttgccgctg
    23461 cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc attatgcagg
    23521 ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc tgtaacctgg
    23581 cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt gccatcgcca
    23641 ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc cgtattcccg
    23701 tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat caggcgcggc
    23761 gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg ctgctcacgg
    23821 cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt ggtttactga
    23881 ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc accggacgta
    23941 cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg atcttcagtt
    24001 acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg gtaattggtg
    24061 gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc tggatgtacc
    24121 gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg atcggctcaa
    24181 cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac tgggacaaaa
    24241 tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat ttgctgctgt
    24301 ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt gcggcaccgc
    24361 agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg atatggtggg
    24421 tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat taaaaaaagg
    24481 tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc cactggatgc
    24541 gcgtaatcac ggctacactg tgctggatat tcagcaagac gggccgacca ttcgttattt
    24601 aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg ttacctctga
    24661 cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag gaggttgcta
    24721 tgtgtggacg ctgtgcccaa tcccaaacgc gtgaggatta ccttgcgctt ctcgcggaag
    24781 atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat gtcgcgccgg
    24841 gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat ccggttttct
    24901 ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg
    24961 cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg
    25021 atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta tctatcgcgc
    25081 tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc gtggtgacga
    25141 agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag atattcatga
    25201 ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc aagacattgg
    25261 tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc agttcacctg
    25321 gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt taattcaacc
    25381 tgtttgttga catcctccac gccctgaatg acgaggcccc ctgctacgtt caggctgtcg
    25441 cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc atctctccac
    25501 aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc gccatttaca tcggcgttcg
    25561 ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt tctttcgctg
    25621 tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca agcacctgac
    25681 cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag ccctgatcca
    25741 gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg ctgaccgtac
    25801 ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc attacgtggt
    25861 ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt gcgatacagg
    25921 aatgcagtcg ctgtattttg cgtttctgct tctgccagtt gttgctgaat ttgaccttgc
    25981 ggcttaactg tcgctgaagt ctcgccagct tcttctggtt tttctgaaaa ctgtttacag
    26041 gctcaaagac tgtgccatct gacagcgagg caagtttagc cacgccagca tccagcccga
    26101 ccattgatgc tgaagggtga accggagttg atacttcact ttctgcctga atactgatgt
    26161 accacttacc gcaggactgg ctgacagtga catttttcac aacacccgtg acctgacggc
    26221 tgttccggta gcgcatccag ccaagttttg gcagaaaaat acggctgttt tcctgatcga
    26281 gcttaacgcc ctgcggatag cggaatgcat cattctgtca ccgctttttg aatcagggga
    26341 acgccgaccg cttctggaaa aagtttttgc aggcctgctc agggtctttc agtgactgtt
    26401 gcaatggctg tgacggagca tctttaatcc attgcgtttc agtggcattt ttccactcaa
    26461 ccaatcagga agccattttc gcgtaaggga tgtctttgtt accggcctta tgattctcat
    26521 tctgacgtgc cagagtacga ctgaaaacga aacgacatgc gcctgcgaag cgcctcattt
    26581 cacgcgcctg ttgaccaccg ggtcttaacc ggaatttgaa tacctgtagt cgtttcacag
    26641 ggcaaattat actttggtct gtgaaaaaaa ctgatatccg acgcggaagg cactgtgttt
    26701 tcctgatgca tgctcaccgg gtctttgtta ccaagtaccg acgccagatt tttgaccatg
    26761 acgcgacaga aaaactacgt acttactttt caaatgtatg tgctgatttt gaagctgaac
    26821 tggttgaaat ggatggcgaa ccagagcacg tccatctgtt aattaactat ccccccaaac
    26881 tggcgatatc cagtctggta aacaaacagc ctcaaaggcg tatcgggtag gttactgcga
    26941 cgggatcggc cagatattgc tgccaggtat taccgcaaag atgttttgtg gcgtcccggc
    27001 tattttgcca gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa
    27061 cagcaaacac ctggtcaggt gaaaaaacga gccttatatc cccgccctga aggacgggct
    27121 tttacggtgc actggataag cctggagatt atctacacct gcatggacat cattgtacta
    27181 tgccccgtac atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt
    27241 atgcgtctca gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc
    27301 gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa ataaatatca
    27361 tgcaaataat ttccatggtt atttttaggc tatttaaatt aaaaaaataa attatgagtc
    27421 aatcataaat aacaaccttt aatataattt gactaccaaa tttatattcg ttcacaaaat
    27481 agtcatgcat tgtgtgcaat agaaacagtt cagataaaaa tagggatcta cttcacaatt
    27541 taaatgagaa actaaaactt acatcttgaa ataatctcat tgattggatg aatatttgta
    27601 gtgcagtgac atcattttta aataatagtt caaataaaag ggctcacgat gaaaaaatta
    27661 acagtggcaa tttctgctgt agctgcatca gtactgatgg cgatgtctgc tcaggcagca
    27721 gagatttaca ataaagacag taacaagctg gatctgtacg gaaaagttaa tgctaagcac
    27781 tacttctctt ctaacgatgc agatgatggc gacacaactt atgttcgtct gggcttcaaa
    27841 ggcgaaactc aaatcaacga tcaactgact ggtttcggtc agtgggaata tgaattcaaa
    27901 ggcaaccgtg ctgaatctca aggttcttcc aaagataaaa cccgtcttgc cttcgctggc
    27961 ctgaaattcg gtgactacgg ctccatcgat tatggccgta actacggtgt agcatacgat
    28021 atcggtgcat ggaccgacgt tctgccagaa ttcggtggcg atacctggac ccaaacagat
    28081 gtgttcatga ctggtcgcac cacaggtgtt gcaacctatc gtaacaacga cttctttggt
    28141 ctggttgatg gtctgaactt tgctgctcag tatcagggta aaaatgaccg cactgacgta
    28201 actgaagcta acggtgatgg tttcggtttc tccactactt atgagtatga aggattcggt
    28261 gtaggtgcaa cctatgctaa atctgaccgc actaatgatc aggttatcta cggtaacaac
    28321 agcctgaatg catctggtca aaatgctgaa gtatgggcag ctggtctgaa atatgatgcg
    28381 aacaacatct atctggctac cacctattct gaaacccaga acatgactgt ttttggtaat
    28441 aaccatattg ccaacaaagc acaaaacttc gaagtagttg cacaatatca gttcgacttc
    28501 ggtctgcgtc cgtccgttgc ttacctgcaa tctaaaggaa aagacttggg tgcgtggggt
    28561 gatcaggacc tgattgaata tattgatgta ggtgcaacct attacttcaa caaaaatatg
    28621 tccacttttg ttgattacaa aatcaacctg attgataaga gcgatttcac gaaagcatct
    28681 ggcgttgcta ccgatgatat cgttgctgta ggattggttt accagttcta atctgattac
    28741 tcaaagatat gttgtgggag gctttgcctc cccaacatat aagtggcgcc ctcaagccac
    28801 tttccttaga agcactatct tgcttctgac tatataaatt accctttatt ttctgggacg
    28861 tttcaacgcc ccatttttaa taatactcag tgaacaatta acgtatcaat tagatttaat
    28921 aacaacgata tccgtaccta acctgatatc taatgtcata agcatccctt taattatccc
    28981 ctcaacctct tgtaaatttc ccactcttga gctccacata gtccaaacgc cggatgcaaa
    29041 aaaaatcaca cgtgcatctc tgaaattgtg atggaggaag tgagtatcaa ttatatgtgc
    29101 ttataaacgt tcccgattgg cacagaagtc accggattgt tcaggctcca ataggttgag
    29161 tatgtcgtat ttattttcca aaataaaaat cgatgctgtt gctccttaat aaaatacttt
    29221 tactctctgg acgcttttct tagctctctt ggtgttatct taaaacgatt atggaacctt
    29281 tcagtaaaac gagaaggaca cttgtaacca ttttccctgg caatctcgct gatgggtttt
    29341 actgtcgttt gtatagcaga caacgcatta tttaacctca cgtcgtccaa tatactttga
    29401 aaacttattc cctcgct
//
LOCUS       DADWTH010000044        29082 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.044, whole genome shotgun sequence.
ACCESSION   DADWTH010000044 DADWTH010000000
VERSION     DADWTH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.044"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="JNY02_002077"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="JNY02_002077"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBB9433613.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="JNY02_002078"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="JNY02_002078"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBB9433614.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JNY02_002079"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JNY02_002079"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBB9433615.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JNY02_002080"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JNY02_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB9433616.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JNY02_002081"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JNY02_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBB9433617.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /gene="radC"
                     /locus_tag="JNY02_002082"
                     /pseudo
     CDS             complement(3421..4089)
                     /gene="radC"
                     /locus_tag="JNY02_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="JNY02_002083"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="JNY02_002083"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBB9433618.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="JNY02_002084"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="JNY02_002084"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBB9433619.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="JNY02_002085"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="JNY02_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBB9433620.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JNY02_002086"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JNY02_002086"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBB9433621.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JNY02_002087"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JNY02_002087"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBB9433622.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="JNY02_002088"
     CDS             8207..9070
                     /locus_tag="JNY02_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBB9433623.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="JNY02_002089"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="JNY02_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBB9433624.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="JNY02_002090"
     CDS             10406..11023
                     /locus_tag="JNY02_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBB9433625.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="JNY02_002091"
     CDS             complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="JNY02_002091"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBB9433626.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12960..13583
                     /gene="gmk"
                     /locus_tag="JNY02_002092"
     CDS             12960..13583
                     /gene="gmk"
                     /locus_tag="JNY02_002092"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBB9433627.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13638..13913
                     /gene="rpoZ"
                     /locus_tag="JNY02_002093"
     CDS             13638..13913
                     /gene="rpoZ"
                     /locus_tag="JNY02_002093"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB9433628.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13932..16040
                     /gene="spoT"
                     /locus_tag="JNY02_002094"
     CDS             13932..16040
                     /gene="spoT"
                     /locus_tag="JNY02_002094"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBB9433629.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16047..16736
                     /gene="trmH"
                     /locus_tag="JNY02_002095"
     CDS             16047..16736
                     /gene="trmH"
                     /locus_tag="JNY02_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBB9433630.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16742..18823
                     /gene="recG"
                     /locus_tag="JNY02_002096"
     CDS             16742..18823
                     /gene="recG"
                     /locus_tag="JNY02_002096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBB9433631.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="JNY02_002097"
     CDS             complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="JNY02_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBB9433632.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLRLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20342..21733
                     /gene="xanP"
                     /locus_tag="JNY02_002098"
     CDS             20342..21733
                     /gene="xanP"
                     /locus_tag="JNY02_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBB9433633.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21854..23563
                     /locus_tag="JNY02_002099"
     CDS             21854..23563
                     /locus_tag="JNY02_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBB9433634.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNREWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="JNY02_002100"
     CDS             complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="JNY02_002100"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBB9433635.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25944..27327)
                     /locus_tag="JNY02_002101"
                     /pseudo
     CDS             complement(25944..27327)
                     /locus_tag="JNY02_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27620..27714
                     /locus_tag="JNY02_002102"
     tRNA            27620..27714
                     /locus_tag="JNY02_002102"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27654..27656,aa:Sec,seq:tca)
     gene            28014..>29082
                     /locus_tag="JNY02_002103"
     CDS             28014..>29082
                     /locus_tag="JNY02_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB9433636.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAH"
BASE COUNT         7182 a         7794 c         7310 g         6796 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccggaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacgcgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gccgctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaactt gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gccccagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccggcaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 gttggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cgctacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccttccagt agtcatcgtc ccactgtttt atttgctgtt gcaggcggga
    12601 aatttcttcc tgtgctctgg ctggcgacca ggccggacag accgcccaca cagatgattg
    12661 ccagcacaag atacttatta atatcgccat ccatactttc atcttcacct ccgctgtagt
    12721 caggctggca gatatacaac gtggtgaagg caaagccgag tggcaaaaac ggagtctgcg
    12781 aggacgcttc ctgagaatcg tctttattgc agtaaatcac aggcaaatgc ggaaacagct
    12841 acgcaaaacg caacaacttt gcgcaaaaag tgtgagcaag ggctacgtca catggccgcg
    12901 ccgtgtataa taagctcgta tgtaggcttt atttcgctaa tcacatacga aagatactca
    12961 tggctcaagg cacgctttat attgtttctg cccccagtgg cgcgggtaaa tccagcctga
    13021 ttcaggcttt attaaaaacc caaccgttgt atgacaccca ggtttctgtt tcacacacca
    13081 cacgccaacc gcgtcctggt gaagtccacg gtgaacatta tttctttgtt aatcatgatg
    13141 aatttaaaga aatgattagc agagatgcgt tcctcgaaca cgcagaagtt tttggtaatt
    13201 actatggcac ttcgcgtgag gccattgagc aagtactggc gaccggtgtc gatgtttttc
    13261 tcgatatcga ctggcagggc gcgcagcaaa ttcgccagaa gatgccgcac gcgcggagta
    13321 tctttatttt accgccgtcc aaaattgaac tggaccgccg tctacgcggt cgcggtcagg
    13381 acagcgaaga ggtcattgca aagcgtatgg cgcaagctgt tgcagaaatg agccattacg
    13441 ccgaatatga ttatctgatt gtgaatgatg acttcgatac cgcgttgacc gatttgaaga
    13501 ccattattcg cgccgaacgt ctgcgcatga gccgccaaaa gcagcgtcat gacgctttaa
    13561 tcagcaaatt gttggcagac tgaacctgat ttcagtatca tgcccagtca tttcttcacc
    13621 tgtggagctt tttaagtatg gcacgcgtaa ctgttcagga cgctgtagag aaaattggta
    13681 accgttttga cctggtactg gtcgccgcgc gtcgcgctcg tcagatgcag gtaggcggaa
    13741 aggatccgct ggtaccggaa gaaaacgata aaaccactgt aatcgcgctg cgcgaaatcg
    13801 aagaaggtct gatcaacaac cagatcctcg acgttcgcga acgccaggaa cagcaagagc
    13861 aggaagccgc tgaattacaa gccgttaccg ctattgctga aggtcgtcgt taatcacaaa
    13921 gcgggtcgcc cttgtatctg tttgaaagcc tgaatcaact gattcaaacc tacctgccgg
    13981 aagaccaaat caagcgtctg cggcaggcgt atctcgttgc acgtgatgct cacgaggggc
    14041 aaacacgttc aagcggtgaa ccctatatca cgcacccggt agcggttgcc tgcattctgg
    14101 ccgagatgaa actcgactat gaaacgctga tggcggcgct gctgcatgac gtgattgaag
    14161 atactcccgc cacctaccag gatatggaac agctttttgg taaaagcgtc gccgagctgg
    14221 tagagggggt gtcgaaactt gataaactca agttccgcga taagaaagag gcgcaggccg
    14281 aaaactttcg caagatgatt atggcgatgg tgcaggatat ccgcgtcatc ctcatcaaac
    14341 ttgccgaccg tacccacaac atgcgcacgc tgggctcact tcgcccggac aaacgtcgcc
    14401 gcatcgcccg tgaaactctc gaaatttaca gcccgctggc gcaccgttta ggtatccacc
    14461 acattaaaac cgaactcgaa gagctgggtt ttgaggcgct gtatcccaac cgttatcgcg
    14521 taatcaaaga agtggtgaaa gccgcgcgcg gcaaccgtaa agagatgatc caaaaaatcc
    14581 tctcggaaat cgaagggcgt ttgcaggaag cgggaattcc ctgccgcgtc agtggtcgcg
    14641 agaagcatct ttattcgatt tactgcaaaa tggtgctcaa agagcagcgt tttcactcaa
    14701 tcatggacat ctacgctttc cgcgtgatcg tcaatgattc cgacacctgt tatcgcgtgc
    14761 tcgggcaaat gcatagcctg tacaagccgc gtccgggtcg cgtgaaagac tacatcgcca
    14821 ttccaaaagc gaacggctat cagtctttgc acacctcgat gatcggcccg cacggtgtgc
    14881 cggttgaggt ccagatccgt accgaagata tggatcagat ggcggagatg ggtgttgccg
    14941 cgcactgggc ttataaagag cacggcgaaa ccagcaccac cgcgcaaatc cgcgcccagc
    15001 gctggatgca aagcctgctg gagctgcaac agagcgccgg tagttcgttt gaatttatcg
    15061 agagcgttaa atccgatctc ttcccggatg agatttacgt tttcacaccg gaagggcgaa
    15121 ttgtcgagct gcctgccggt gcaacgcccg tcgacttcgc ttatgcagtg cataccgata
    15181 tcggtcatgc ctgcgtgggc gcacgcgttg accgccagcc ttacccgctg tcgcagccgc
    15241 ttaccagcgg tcaaaccgtt gaaatcatta ccgcaccggg cgctcgcccg aatgccgcgt
    15301 ggctgaactt tgtcgttagc tcaaaagcgc gcgccaaaat tcgtcagttg ctgaaaaacc
    15361 tcaagcgtga tgattctgta agcctgggcc gtcgtctgct caaccatgct ttgggtggta
    15421 gccgtaagct gaatgaaatc ccgcaggaaa atattcagcg cgagctggat cgcatgaagc
    15481 tggcaacgct tgacgatctg ctggcagaaa tcggacttgg taacgcaatg agcgtggtgg
    15541 tcgcgaaaaa tctgcaacat ggggacgcct ccattccacc ggcaacccaa agccacggac
    15601 atctgcccat taaaggtgcc gatggcgtgc tgatcacctt tgcgaaatgc tgccgcccta
    15661 ttcctggcga cccgattatc gcccacgtca gccccggtaa aggtctggtg atccaccatg
    15721 aatcctgccg taatatccgt ggctaccaga aagagccaga gaagtttatg gctgtggaat
    15781 gggataaaga gacggcgcag gagttcatca ccgaaatcaa ggtggagatg ttcaatcatc
    15841 agggcgcgct ggcaaacctg acggcggcaa ttaacaccac gacttcgaat attcaaagtt
    15901 tgaatacgga agagaaagat ggtcgcgtct acagcgcctt tattcgtctg accgctcgtg
    15961 accgtgtgca tctggcgaat atcatgcgca aaatccgcgt gatgccagac gtgattaaag
    16021 tcacccgaaa ccgaaattaa tgttttatga acccaacacg ttatgcacgc atctgcgaaa
    16081 tgctcgccag gcggcagcct gatctgaccg tctgcatgga gcaggtccac aaacctcata
    16141 acgtttctgc gattattcgt accgcagatg ccgttggcgt acatgaagtt cacgccgtct
    16201 ggcctggtag ccgcatgcgc accatggctt cggcagcggc gggtagtaac agctgggtac
    16261 aggtgaaaac acaccgcacc attggcgatg ccgtcgctca tctcaaaggc cagggcatgc
    16321 agattctggc aacccatctt tctgataatg ctgtcgattt ccgcgaaatt gattacactc
    16381 gcccgacctg cattttgatg ggacaggaga aaacgggcat cacgcaggaa gcattggccc
    16441 tggcggatca ggacatcatc attccgatga tcggcatggt gcagtcgctg aatgtttccg
    16501 ttgcctcagc cctcattctt tacgaagccc agcgtcagcg gcaaaatgca ggcatgtacc
    16561 tgcgtgaaaa cagcatgttg ccggaagcag agcaacaacg cctgttgttt gaaggcggct
    16621 atccggtgct ggcgaaagtc gcaaaacgca aaggcctgcc ttatccccac gtcaatcagc
    16681 aaggcgagat cgaagctgat gccgactggt gggctactat gcaggctgca gggtaagtgc
    16741 catgaaaggt cgcctgttag atgctgtccc actcagttcc ctaacgggcg ttggcgcagc
    16801 acttagtaac aaactggcga aaatcaacct gcataccgtg caggatctac tcttacacct
    16861 tcccctgcgc tacgaagatc gcacccatct ctaccccatc ggagaactac tgccgggcgt
    16921 ttatgccacg gtggaaggcg aagtgctgaa ctgcaatatc tccttcggcg gtcggcggat
    16981 gatgacctgc cagatcagcg acggttccgg catcctcacc atgcgctttt tcaatttcag
    17041 cgcggcaatg aaaaatagcc tggcgacggg ccgccgtgta ctggcttatg gcgaagcaaa
    17101 gcgcggtaaa tatggtgcgg agatgatcca cccggaatac cgcgtgcagg gcgatctcag
    17161 cacgccagaa ttacaggaaa cgctcacgcc ggtttatcca acaacggaag gcgtaaagca
    17221 ggccacgctg cgtaaattaa ccgaccaggc gctggatctg ctcgacacct gcgccattga
    17281 agaactcctg ccgccggaac tgtcacaagg aatgatgacg ctaccggaag cgttgcgcac
    17341 tttgcaccgc ccgccaccga cgctacagct tagcgatctg gaaaccgggc agcatccggc
    17401 gcaacgtcgt ctgattctgg aagaactgct ggcgcacaac ctcagcatgt tagccttacg
    17461 tgccggagca cagcgttttc atgcccagcc gctgagcgcc aatgacacgc tgaaaaataa
    17521 actcctcgcc gccttaccgt tcaagccaac gggcgcacag gcacgcgtag tggcggagat
    17581 cgagcatgat atggcgctgg atgtgccgat gatgcgcctg gtacagggcg atgtaggttc
    17641 cggtaaaacg ctggtcgccg ccctcgccgc gctgcgtgcg attgcccacg gcaaacaggt
    17701 ggcactgatg gcaccaaccg aattactcgc cgagcagcac gccaataact tccgcaactg
    17761 gtttgcaccg ctcggtatcg aagtgggctg gctcgccggt aagcagaaag gcaaagcgcg
    17821 actgtctcag caggaagcca tcgccagcgg tcaggtgcag atgattgtcg gtacacacgc
    17881 catcttccag gaacaggtgc agtttaacgg cctggcgctg gtgattatcg acgaacagca
    17941 tcgttttggc gtgcatcagc gtctggcatt gtgggagaaa ggccagcagc agggcttcca
    18001 tccgcatcag ttgatcatga ccgccacgcc gatcccccgc acgctggcaa tgacggcgta
    18061 tgccgatctc gatacctcgg tgatagatga gctgccgcca ggccgcacgc cagtgactac
    18121 ggtcgctatt cctgataccc gccgtaccga catcattgac cgcgtgcgcc acgcctgcat
    18181 aactgaaggt cgtcaggcgt actgggtttg tacgttgatt gaagagtcag agttactgga
    18241 agcgcaggcg gcggaagcta cctgggaaga gttgaaactg gcgctaccag aattgaatgt
    18301 tggcctggta cacgggcgga tgaaaccagc cgagaaacag gcggtgatgg cgtcgtttaa
    18361 acaaggtgag ctacatctgc tggttgccac taccgttatt gaagtcggtg ttgatgtgcc
    18421 taacgccagc ctgatgatta tcgaaaaccc tgagcgtctg ggtctggcgc agttacacca
    18481 gctgcgtggg cgcgtaggtc gtggcgcggt ggcttctcac tgcgtgctgc tctacaaaac
    18541 gccgctttct aaaacggcgc aaattcgcct gcaagtgctg cgcgacagta acgacggttt
    18601 tgtgattgcg caaaaagatc tggagattcg cggccctggc gaattgttag gcacgcgtca
    18661 gacgggtaat gctgaattta aagtggcgga tttactgcgc gatcaggcga tgatcccgga
    18721 agttcagcgc ctggcgcgac atattcacga acgttaccca caacaggcaa aagccttgat
    18781 agaacgctgg atgccggaga cagaacgtta ctcgaatgcg taaaaggcgg cggtgagaag
    18841 accgccgctt catcggttaa ccggcaaaaa tcggcaacat taaatacagc ttaatcacca
    18901 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    18961 gcgacgggcc aaagcgttca gtgatcgcct gcatgttggc gattgccgtt ggcgttgcac
    19021 cgaggccaaa accacagtga cccgcagcca gcactgccgc atcgtagttt ttgcccatca
    19081 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    19141 ccagcatcgg cagtgccagc gaagccagct cccacagtct cagccccatc aacgccatcg
    19201 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    19261 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    19321 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    19381 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    19441 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    19501 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    19561 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    19621 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    19681 acagccccat cagcggatca agccctaaca ggctagccat accaatacca atggcatttt
    19741 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    19801 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    19861 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    19921 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    19981 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    20041 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    20101 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    20161 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    20221 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    20281 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    20341 aatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    20401 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    20461 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    20521 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    20581 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    20641 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    20701 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    20761 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    20821 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    20881 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    20941 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    21001 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    21061 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    21121 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    21181 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    21241 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    21301 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    21361 gatccagttg actggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    21421 cgtgctgggt ctgttcccgg cagtgagcga ttttgtacaa cacattccag aaccggttct
    21481 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    21541 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    21601 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    21661 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    21721 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctctttc
    21781 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    21841 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    21901 agtggtgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    21961 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg atcaccgttt
    22021 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gtcagcccgc
    22081 gcccctggtg gcaaaaagtg tcgacattgc gctaagcagt cgccaactga ccgaaccacg
    22141 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    22201 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    22261 agaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    22321 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    22381 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    22441 gactaacctg ggtgccgaca tcgcccgcgg gacattaacc ggaaacgcgc agcgtaacgc
    22501 cgacggcagc tggcaagtgg aaaatctgcg catggcggat atccgtctac aaagcgaaaa
    22561 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgctt
    22621 ggaagtcatc gatgctcgtc tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    22681 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caggaaggca aactgtcgat
    22741 gaacgccagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    22801 attttccccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    22861 cagaacgtca gggaactggc tgcgtgacgg taaaacgttg atccttgatg atgcggcaat
    22921 tgccgggctg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    22981 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    23041 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    23101 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    23161 cgcgacattc aatcgtgttg atgttcgtcg cccgtcgctg gcgctgaccg ccaacagcag
    23221 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    23281 aagtgtttca caaacgccac aacgtcagac ccatatcagc ctgaatggac gcggtgtgcc
    23341 ggtgaatatt ttgcaacaat ggggatggcc tgaattacag ttgactggcg acggcaatat
    23401 tcagcttacc gccagtggag atattcaggc caatgccccg ctgaaaccta cggttagcgg
    23461 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    23521 cgtttccagc ggtgaagtta catcgacgga gccggtgcgg taaacatcaa tctcgccggg
    23581 tacgactcag acgtacccgg cattccgtca atagattaca acgtaattgt cagcgcattc
    23641 ccttgaggcg tcaccaccag cccctgctca ctttcagcct gcgaaccgcc ttgcagacca
    23701 tttactttca caacattgcg caagcacagc gtccagttct tcgcctcgcc cgcaccagtc
    23761 acagtaatcg tgttgccagt acgtgctgct tttaaagtaa agatcaccga tccgtcagca
    23821 gcaggcactt cacagacggc ttcatgcccg tcttgcagat taaagaggtg gaatgccgtg
    23881 ccttcgtgcc acacgtaatc gggacgttga tcgttgttgc ccagcgccag tagagtgtta
    23941 tcacgcacat aaacgggcag actcaggaag ccgtgctgct gtttatgcca gcgactaccg
    24001 tcgagttcat cgttgtgcca caggtgtgtc cagcgacctt ccggcaggta gaactgcaca
    24061 tcgcccgctt cagtgaacac cggcgcaacc atcacgttgt cgcctaacat gtattgacgg
    24121 tcaaggtaat cacaagccgg atcgtccggg aactccatca tcatggcccg catcatcggc
    24181 gtaccccgcg cgttcgcacg cgcagcttca cgatacagat acggcatcat gcggcatttc
    24241 agttgcgtga agaagcgcac cacatcacag gactcatcat cgtaggccca cggcacacga
    24301 taagatttgc taccgtgtaa acggctatgg ctggagagca aaccaaacgc gcaccagcgt
    24361 ttgtaaacgt gcgccggagc ggtattttca aagccgccga tatcgtggct ccagaagcca
    24421 aaacctgaaa ggccaataga caaaccaccg cgcaggcttt ccgccattga ttcgtagtta
    24481 gcgtaacaat cgccacccca gtgtaccggg aatttctgcg caccgacgga ggccgagcgg
    24541 gcaaacaaga cagcttcttc ctcaccaacg gtgtccttga gcacgttcca caccagttcg
    24601 ttgtagatgt acgcataatg gttatgcatt ttctgcggat cggaaccgtc aaaccactga
    24661 acatcagttg ggatacgttc gccaaagtcg gtcttaaagc aatcaacgcc catcgcgacc
    24721 agacctttca gtttgtcggc gtaccatttg caggcatccg gattggtaaa gtcataaatc
    24781 gccagacctg gctgccattt atcccactgc catagcgaac cgtccgggcg tttgagtaaa
    24841 tagcctttct cttgtaactc tttaaagacg ggggattttt gaccgatata ggggttaatc
    24901 cagacgcaga ttttcagccc tttcgctttc aggcggcgga tcatcccttc cgggtcaggg
    24961 aaagtcagcg ggtcccactc aaaatcgcac cactggaagg ctttcatcca gaaacagtca
    25021 aagtggaaaa catgcagcgg cagattgcgt tccgccatac catcgataaa gctgtttacc
    25081 gtcgcttcgt cgtagttggt ggtaaatgaa gtggttagcc acaggccgaa ggaccacggg
    25141 ggcggcagcg ccggacgacc agtaaagcgg gtataacgat caagtaccgc tttcggcgtc
    25201 gggccgtcga taacaaagta ttcgagatat tcactctcaa cgctgaactg cactttggag
    25261 actttctccg atcccacttc aaaagagaca cactggggat gattgaccag taccccataa
    25321 ccacggttag tcatgtagaa cgggatattt ttatacgcct gttcagtact tgtgccgccg
    25381 tcccggttcc aggtctctac cgtctggcca ttgcgcacca gggcagtaaa gcgctctccc
    25441 agaccgtaaa ctgtttcgcc aacgccaaga tcaagccgct caaacatata attgcgttga
    25501 ttattcgtgt cctgcacgta gccattattt ttcacctgac taccggtaat acgttcgccg
    25561 ttgcgcagaa aatccagtga ccagaactca cctttgctga cacgcgcgct taagttgcca
    25621 cttttaaact cagcataacg ttctgtgttt tcgattgtga ccttcacgtc ctgcaaaata
    25681 ttgagcggat aatgaggacc gttattcagc gccccctgaa aatactcaat ccgcacaccg
    25741 acaatacctt cctgtgggga gaaaaagcgc agcgtaaata aaggcgtatc aagctgccag
    25801 gtacgttcac gcacatcacg gggggcagca tagaccacca tttcattatc ctgctgttca
    25861 acctcgaata cctgaagcgg gtgaatcaaa ttgaggccag gttgaatcaa ccagtttcca
    25921 tcgctaattt tcattgccgt tccttagttc tgcacttctt gagaggtgaa ttgttgctgg
    25981 caacggcgtt taccctgagc cagctgttcc ataacggttt tcagattgtg cgtcgtgagt
    26041 gagtagtagc gtttagcgat aatcgcgctc agcaaataac agatcgccgg aacaatcgtg
    26101 aatagcgcaa taatgatgct aatcgtggcg ctgttctgcg ctttttctgc cgcatcatat
    26161 ccgccataag ccagcatcca gccgataaga gcgccgccga aggccaaccc cagtttgagc
    26221 acaaacagcg tgccagcaaa actgatcccg gtcaggcgtt taccattgca ccactcgccg
    26281 tagtcgacgg tatcgaacat cattacccac tggataggtg tcaccagttg atgcaacaca
    26341 ccaatcacga agatgaagac aaacatagtg atgctggcct gcatgggaac aaagaacatc
    26401 gcgaggctaa tcactgccag cagggcgttc gtccaccaga agatagtgac tttacatttc
    26461 cagtcggtca gaggttttgc cagtgcggaa ccaatcaggt tacccacgca ataagtggtg
    26521 agaaaagcga caaacacttc cggcgtgccc aaaatccatg tgacgtaata catcatcgcc
    26581 ccaccgcgta cgcacaccgc caggatattg aaaatggtta gtaaaccgac aatccgccac
    26641 tggtcgtttt gccagatatc acgtaaatct tcccgcatag acgttgttgt aggtggtgct
    26701 tcaacgcgtt ctttagtggt gaagaaacaa aatgccagca tcatgaatgc caccacggaa
    26761 aggaccacga taccgccctg gaaaccgagt ggtttattat caccgccaat taaattaacc
    26821 agtggcatca tcagaacagt agaaagcatg cctcccgcgg tcgccagcac aaaacgccag
    26881 gattgcagcg agatacgctg agtcgggtca ttggtgatta caccacccaa tgcgcagtaa
    26941 gggatattga cgacggtata aagtaaggta agttagggtg taagtaattg ctgcatagat
    27001 cattttgccg ttcatactga gatctggcgt gctataggcc agtacacaga cgatcccgaa
    27061 tggcagtgcg ccaaacagta cccacggacg aaatttaccc cagcgagagc gcgttcgatc
    27121 ggccaacaac cccatgcaag gatcggaaat cgcatccagt gcacgagcga ccaaaaacat
    27181 ggttccgaca aatccggcag gaatgccaaa aatatcggta taaaagaaca tcatatataa
    27241 cattacgtta tcgaaaataa tgtggctggc ggcgtctccc atgccataac caattttctc
    27301 tttaacggac aacacttcac tcttcatctg cttttccttg atgctatagg ggtgctgaga
    27361 ccgataacgc attttgtaaa ccatccctgt gagagcgaat attccgatat ctggttatta
    27421 atttatgatt cttgttttat gtgatcgtgg tagcgttaat tccgctcata tatcattgta
    27481 aaatatgggt tttatataaa ttataatgct ttcgtgataa tacgctgcgt ttattaggcg
    27541 gaaaaaactg atctggggga tgtagaaact caaggaagta gctataatgc gccccgcctc
    27601 catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg aggcggctgg acttcaaatc
    27661 cagttggggc cgccagcggt cccgggcagg ttcgactcct gtgatcttcc gccagtttac
    27721 ctcttctgaa ctctcccaat gtcaataaat ctcttctaat cccttgtata tcagtattta
    27781 tttgtatcct gaggtcaacc tacttcaccc cgtatcaagt ttcttttggg ggtactttag
    27841 ggggtgttat ttcctattga aatacaagca cccccataat aggaaaaatg tgatacataa
    27901 caacggcttt taacaatatc tttgttatac aaggggtgta ttgggggtat caggttagtt
    27961 actggcagaa atccccctca ggaacatgaa ttcatcaaaa gccggaggat gtcatggcat
    28021 taaccgatat caaagtgaaa accgcaaaac ccaaagacaa accctataag cttgctgatg
    28081 gcggcggtat gtatctactg attaacacta atggttctaa atactggcgc atgaagtacc
    28141 gctttgctgg taaagaaaag atgctttcta ttggcgtata ccccgatgta acgctagccg
    28201 atgctcgcga gaaacgcagt gaagccagga aactcctcgc tgctggaggc gatccagggg
    28261 aggcaaaaaa ggaagaaaaa atcgcgcagc aaatgagtct gaaaaatacg ttcgaggcta
    28321 ttgctcgcga atggcaccag ttaaaagctg atcggtggtc attacgctat cgtgatgaaa
    28381 tcattgatac ctttgaaaaa gacatttttc cctacattgg caagcgccct attgccgaaa
    28441 tcaaaccaat ggagttactg gaagcactgc gtaaaatgga aaaacgtgga gctctggaga
    28501 aaatgcgcaa ggtccgccag cgctgtggtg aggtgttccg ctatgcaatt gttactggcc
    28561 gggcagacta taacccagcc cccgatcttg ccagtgcttt agctacaccg aaaaaagtac
    28621 attttccctt tcttactgct aatgaacttc cccacttcct caacgatctg gcgggttata
    28681 ccggaagtat aatcactaag acagctactc agatcattat gttaaccggc gtacgaacac
    28741 aagaactgcg ttttgcacgc tgggaagata tcgattttga gacaaaattg tgggagatcc
    28801 cggcagaagt aatgaagatg aagcgacctc atattgtgcc gctctctgag caggtcatta
    28861 tgctatttaa gcagcttgaa ccaatctcaa aacatcatcc cctagtcttt atcggtagaa
    28921 atgatccccg caaaccaatt agtaaggaaa gcattaacca agtaattgaa ttacttggat
    28981 ataagggaag actcacaggg cacggtttca gacacaccat gagtacaatt ctgcatgaac
    29041 aaggctttaa ttccgcctgg attgaaatgc agttagctca tg
//
LOCUS       DADWTH010000045        28463 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.045, whole genome shotgun sequence.
ACCESSION   DADWTH010000045 DADWTH010000000
VERSION     DADWTH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.045"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="JNY02_002104"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="JNY02_002104"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBB9433637.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNY02_002105"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNY02_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBB9433638.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="JNY02_002106"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="JNY02_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBB9433639.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /gene="ghxP"
                     /locus_tag="JNY02_002107"
                     /gene_synonym="yjcD"
     CDS             2778..4127
                     /gene="ghxP"
                     /locus_tag="JNY02_002107"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBB9433640.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="JNY02_002108"
     CDS             4278..5927
                     /locus_tag="JNY02_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBB9433641.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="JNY02_002109"
     CDS             complement(6081..7373)
                     /locus_tag="JNY02_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBB9433642.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNY02_002110"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNY02_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBB9433643.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVMLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIISFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="JNY02_002111"
     CDS             complement(9197..9511)
                     /locus_tag="JNY02_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBB9433644.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNY02_002112"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNY02_002112"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBB9433645.1"
                     /translation="MSQIHKHTIPANITDRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="JNY02_002113"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="JNY02_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBB9433646.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAVLDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="JNY02_002114"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="JNY02_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBB9433647.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="JNY02_002115"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="JNY02_002115"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBB9433648.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="JNY02_002116"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="JNY02_002116"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBB9433649.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAVCGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="JNY02_002117"
     CDS             15750..17468
                     /locus_tag="JNY02_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBB9433650.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLAAFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="JNY02_002118"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="JNY02_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBB9433651.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="JNY02_002119"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="JNY02_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBB9433652.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="JNY02_002120"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="JNY02_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBB9433653.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="JNY02_002121"
     CDS             complement(20170..20859)
                     /locus_tag="JNY02_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBB9433654.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNY02_002122"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNY02_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNY02_002123"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNY02_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBB9433655.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNY02_002124"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNY02_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNY02_002125"
     CDS             complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNY02_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBB9433656.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGDDSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAALEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27862..28137)
                     /locus_tag="JNY02_002126"
     CDS             complement(27862..28137)
                     /locus_tag="JNY02_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433657.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28346..>28463)
                     /locus_tag="JNY02_002127"
     CDS             complement(28346..>28463)
                     /locus_tag="JNY02_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433658.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6485 a         7207 c         7641 g         7130 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcagtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttggtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctagagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag attatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcgg ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tccttttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagctgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcatcaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgt gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg tgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggtgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaacttaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cggcgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgtgct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttcga gcgccgcacg ttgcgccacc agcgcatcga cgccgctgac
    27301 ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt ctgcggcgcg
    27361 ctgcgccgtt cttgcacggt caacgtcttc tgctgagaca aaaccctctt tcagtaatgg
    27421 ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt
    27481 ggcattaacc gagtcgtcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat
    27541 ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg
    27601 cgggtcgatg cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag
    27661 ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc
    27721 gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc
    27781 caacgcaacc actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc
    27841 catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc
    27901 aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac
    27961 tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata
    28021 ataagcgccg atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg
    28081 aattatccag gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt
    28141 aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg
    28201 tcagtatggc tattttctcg atgaataaaa atagctttat tcagccaccg cattcataac
    28261 ttatatattt tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct
    28321 tcagcgcccc caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga
    28381 aattatccag acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc
    28441 tcttcgcttt caccagatcc gcc
//
LOCUS       DADWTH010000046        28092 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.046, whole genome shotgun sequence.
ACCESSION   DADWTH010000046 DADWTH010000000
VERSION     DADWTH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.046"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..192)
                     /locus_tag="JNY02_002128"
     CDS             complement(<1..192)
                     /locus_tag="JNY02_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB9433659.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
     gene            386..1549
                     /gene="yqhD"
                     /locus_tag="JNY02_002129"
     CDS             386..1549
                     /gene="yqhD"
                     /locus_tag="JNY02_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBB9433660.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKVAR"
     gene            1654..2481
                     /gene="dkgA"
                     /locus_tag="JNY02_002130"
     CDS             1654..2481
                     /gene="dkgA"
                     /locus_tag="JNY02_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBB9433661.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDEPGKIAKLDQGKRLGPDPDQFGG"
     gene            2681..3607
                     /locus_tag="JNY02_002131"
     CDS             2681..3607
                     /locus_tag="JNY02_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBB9433662.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            3658..3915
                     /gene="yqhH"
                     /locus_tag="JNY02_002132"
     CDS             3658..3915
                     /gene="yqhH"
                     /locus_tag="JNY02_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBB9433663.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(3958..6177)
                     /locus_tag="JNY02_002133"
     CDS             complement(3958..6177)
                     /locus_tag="JNY02_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBB9433664.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSLINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(6288..7700)
                     /gene="ftsP"
                     /locus_tag="JNY02_002134"
     CDS             complement(6288..7700)
                     /gene="ftsP"
                     /locus_tag="JNY02_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBB9433665.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(7775..8512)
                     /gene="plsC"
                     /locus_tag="JNY02_002135"
                     /gene_synonym="parF"
     CDS             complement(7775..8512)
                     /gene="plsC"
                     /locus_tag="JNY02_002135"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBB9433666.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(8746..11004)
                     /gene="parC"
                     /locus_tag="JNY02_002136"
     CDS             complement(8746..11004)
                     /gene="parC"
                     /locus_tag="JNY02_002136"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBB9433667.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(11550..12032)
                     /locus_tag="JNY02_002137"
     CDS             complement(11550..12032)
                     /locus_tag="JNY02_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB9433668.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(12085..12477)
                     /gene="ygiW"
                     /locus_tag="JNY02_002138"
     CDS             complement(12085..12477)
                     /gene="ygiW"
                     /locus_tag="JNY02_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBB9433669.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            12629..13288
                     /gene="qseB"
                     /locus_tag="JNY02_002139"
     CDS             12629..13288
                     /gene="qseB"
                     /locus_tag="JNY02_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBB9433670.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLSSDFIRTVHGIGYTLGE
                     K"
     gene            13285..14634
                     /gene="qseC"
                     /locus_tag="JNY02_002140"
     CDS             13285..14634
                     /gene="qseC"
                     /locus_tag="JNY02_002140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBB9433671.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTPDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLIGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLLALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14680..15012)
                     /locus_tag="JNY02_002141"
     CDS             complement(14680..15012)
                     /locus_tag="JNY02_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBB9433672.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            15331..15912
                     /gene="mdaB"
                     /locus_tag="JNY02_002142"
     CDS             15331..15912
                     /gene="mdaB"
                     /locus_tag="JNY02_002142"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBB9433673.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            15943..16257
                     /locus_tag="JNY02_002143"
     CDS             15943..16257
                     /locus_tag="JNY02_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBB9433674.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(16305..18197)
                     /gene="parE"
                     /locus_tag="JNY02_002144"
     CDS             complement(16305..18197)
                     /gene="parE"
                     /locus_tag="JNY02_002144"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBB9433675.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(18226..18807)
                     /gene="yqiA"
                     /locus_tag="JNY02_002145"
     CDS             complement(18226..18807)
                     /gene="yqiA"
                     /locus_tag="JNY02_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBB9433676.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(18807..19634)
                     /gene="cpdA"
                     /locus_tag="JNY02_002146"
     CDS             complement(18807..19634)
                     /gene="cpdA"
                     /locus_tag="JNY02_002146"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBB9433677.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(19659..20081)
                     /locus_tag="JNY02_002147"
     CDS             complement(19659..20081)
                     /locus_tag="JNY02_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBB9433678.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(20082..20711)
                     /gene="nudF"
                     /locus_tag="JNY02_002148"
     CDS             complement(20082..20711)
                     /gene="nudF"
                     /locus_tag="JNY02_002148"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBB9433679.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            20916..22397
                     /gene="tolC"
                     /locus_tag="JNY02_002149"
     CDS             20916..22397
                     /gene="tolC"
                     /locus_tag="JNY02_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBB9433680.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            22545..23216
                     /locus_tag="JNY02_002150"
     CDS             22545..23216
                     /locus_tag="JNY02_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBB9433681.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            23222..24382
                     /locus_tag="JNY02_002151"
     CDS             23222..24382
                     /locus_tag="JNY02_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBB9433682.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(24420..25208)
                     /gene="ygiD"
                     /locus_tag="JNY02_002152"
     CDS             complement(24420..25208)
                     /gene="ygiD"
                     /locus_tag="JNY02_002152"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBB9433683.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            25351..26124
                     /gene="zupT"
                     /locus_tag="JNY02_002153"
     CDS             25351..26124
                     /gene="zupT"
                     /locus_tag="JNY02_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBB9433684.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(26182..26352)
                     /locus_tag="JNY02_002154"
     CDS             complement(26182..26352)
                     /locus_tag="JNY02_002154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433685.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(26613..27266)
                     /gene="ribB"
                     /locus_tag="JNY02_002155"
     CDS             complement(26613..27266)
                     /gene="ribB"
                     /locus_tag="JNY02_002155"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBB9433686.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(27371..27519)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27641..27931
                     /gene="ubiK"
                     /locus_tag="JNY02_002156"
     CDS             27641..27931
                     /gene="ubiK"
                     /locus_tag="JNY02_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBB9433687.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         7095 a         7454 c         6954 g         6589 t
ORIGIN      
        1 attgatataa ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac
       61 cggtgtacgt gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga
      121 caaactattt tctttatttt tcagtttatt aactttatcc gccagcaagc gacaaatctc
      181 ttcacgtttc atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt
      241 ttgtagcatt tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg
      301 agagccaggg cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat
      361 attggcccag caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat
      421 tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt
      481 attgattacc tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga
      541 tgccctgaaa ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga
      601 aacgctgatg aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt
      661 tggcggcggt tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga
      721 aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc
      781 gatgggctgt gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat
      841 ctcccgtaaa accacaggcg acaagcaggc gttccattct gcacatgttc agccggtatt
      901 tgccgtgctc gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt
      961 agtggacgcc tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat
     1021 tcaggaccgt ttcgcagaag gcattttgct gacgctaatc gaagatggtc cgaaagccct
     1081 gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct
     1141 gaacggtttg attggcgctg gcgtaccgca ggactgggca acgcatatgc tgggccacga
     1201 actgactgcg atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact
     1261 gtggaatgaa aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg
     1321 gaacatcact gaaggttccg acgatgagcg tattgacgcc gcgattgccg caacccgcaa
     1381 tttctttgag caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc
     1441 catcccggct ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca
     1501 tgacattacg ttggatgtca gccgccgtat atacaaagtc gcccgctaag ctttttacgc
     1561 ctcaaacttt cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc
     1621 ctgctccggt gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta
     1681 caggatggca atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa
     1741 gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg
     1801 gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa
     1861 gaactgttca tcaccactaa gctgtggaac gacgaccaca agtgcccccg cgaagccctg
     1921 ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc
     1981 gttcccgcta tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag
     2041 ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt
     2101 gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa
     2161 cgccagctac acgcctggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta
     2221 gcgcaaggag gaaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac
     2281 ggcaaaaccc cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc
     2341 ccgaaatcgg tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc
     2401 gacaaagacg aacccggcaa aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat
     2461 cctgaccagt tcggcggcta acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg
     2521 gacttttcag aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca
     2581 ggaatgccaa atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca
     2641 tctatatccg ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt
     2701 tttagcagcc ctggcaagtt ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca
     2761 aacagtccgg cagatttatc agaactataa atcagatgcc agtacgcctt atttcggtga
     2821 aaccggagag cgggcgataa cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa
     2881 tcttacacta ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga
     2941 ttttggcgat ctggtgttag aaagcgtagc gataacccaa actgacgtcg atcatgccga
     3001 tgccgttgtg cgctttcgta tctttaaaga tgataaagaa aagaccacac agacactgaa
     3061 aatggtggcg gaaaatggtc gttgggtcat tgatgatatt gtcagcaatc atggcagcgt
     3121 cttacaagca gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcagaa
     3181 agaacagcca gaatcctttg ttgctgaact ctttgaacat attgctgatt atagctggcc
     3241 gtggacgtgg gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac
     3301 cttcaagacg gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga
     3361 caatccgatc tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct
     3421 ggagaaaacc gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa
     3481 tgaagagcaa gagctggttt tacagcggcg cgaaggtaag tgggaaatcg ctgattttat
     3541 ccgcccgaac agcggcagcc tacttaagct gattgaggca aaaaccgccg ccagattaaa
     3601 gcaatgagct gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg
     3661 aaaacgattt tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc
     3721 aatgagcaaa aggtcaatca actggcgagc aatgtacaaa cattaaatgc caaaatcgcc
     3781 cggcttgagc aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct
     3841 aacagagcca atacgcgtct tgatgcacag gactattttg attgcctgcg ctgcttgcgt
     3901 atgtacgcag aatgataaaa aaatccccgg cagcatgtca gttgccgggg atttttttta
     3961 acgtccaacc gccgctttag ggcgtttctt cgcaccagca ttcaccggac gagattgcgt
     4021 agacgacgct ttttttgccg tagcaggcgt ctgacgctga gtcgccatcg gcgtatgttt
     4081 agtcaacgcc ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt
     4141 tggcgcaggc actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc
     4201 cagcgcctgg cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg
     4261 caaacgacgc tgtttgtcgc ccttcggtac gaagacgtct tcgctcttat aaccaatctt
     4321 cgccagcggt tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta
     4381 gaagttctgt acctggtcga gacggaaacg atgctttttc agccacagcg ccaaattcac
     4441 catatcttca tcacgcgtac cggggtgcgc agagatgaaa tacgggatca gatactgctc
     4501 tttacctgcc tgtttcgagt aggtatcgaa cagctcttta aagcggtcat agctgcccat
     4561 gcccggcttc atcatcttcg acaacggccc ttcttcggta tgttccggag caatcttcag
     4621 ataaccgccg acgtgatggg tcgccaactc tttgatatag cgcggatctt ccacggcgat
     4681 gtcataacgt acgcctgagg cgataaggat ttttttaatg cctttcagat cacgcgcacg
     4741 gcgatagagg ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa tatccggata
     4801 aacgcacgac aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata
     4861 catgttggca gttggcccac cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc
     4921 gcggatcgct tcgatctcat taatgagcga atcttccgaa cggctctgaa taatgcgccc
     4981 ttcgtgctcg gtaatagaac agaaagaaca gccgccaaag cagccgcgca taatgttgac
     5041 cgaaaaacgg atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac
     5101 gcgcttgtac ggcagcgcaa aaacgcagtc catctcttcg gtagaaagcg ggatagcagg
     5161 cgggttgatc cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg
     5221 gttggtttcg tggtgcagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac
     5281 tttctcgaaa gaaggcagca atacgtaggt tttttcccac ggtttctggc gcggtggctg
     5341 cacgattaca gctttcgctt cctgcttttt cggcgcaaca ggtttgttat ccgcacacgg
     5401 caaatcttcg ccatacggat gcgggattgg gtcgattttc cctggggtat caagacgggt
     5461 ggaatccaca ccgctccagc caggcagcgc ctcttttacg ataattgcgg tattacgcac
     5521 atcgcggatt tcactaatcg gctcgcccat cgccagacga tgcgctactt ccaccagcgg
     5581 acgttcaccg ttaccaaaca tcagcatgtc ggctttcgaa tctaccagaa cggaacggcg
     5641 cacggtatcg gaccagtaat cataatgcgc ggtacggcgc aggctagcct caataccgcc
     5701 gaggatcacc ggcacatctt tccacgcctc tttacaacgc tgggtataaa ccagcgtggc
     5761 gcgatccggt cgcttacccg cgacattatc tggcgtgtag gcatcatcat gacgtaaacg
     5821 gcgatcggcg gtatagcggt tgatcatcga atccatgttg ccagcagtaa caccaaaaaa
     5881 cagattcggt ttacccagac gcataaagtc gtctttgctg ctccagtccg gctgggcgat
     5941 gatcccgacg cgaaagccct gcgcttccag catacgaccg caaatcgcca tcccgaagct
     6001 tgggtgatcg acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag
     6061 ttgatccatc tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca
     6121 gtactgcggc caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt
     6181 gcttccagaa atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag
     6241 aaatcgaaag gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga
     6301 ttgaccaaca gttgcccaat cgagccacgg tccgccattt ccagcgtctg actgttgaag
     6361 tagaacggga agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg
     6421 tcaacccaaa cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg
     6481 ttacggatct ggaacattac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc
     6541 gtccagcgtt cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc
     6601 tgtccattaa tacccgggtc atcacccaga ctgatatcgc gactgcgaat tggcgaacca
     6661 gccatgattt cagttggcag caagcgcatc ggaagactgt ctgtgaccag cggcagaagg
     6721 ccggttgggc gcagcgttag caccaaggta gaaaccagaa tactggatgg ctcaaagaag
     6781 ccgcgaatac gatcaacaat gctcgccgct tcgccacagg tgatcgacac ttcatcgccg
     6841 ttgctcatat ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc
     6901 gatacaggag caggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg
     6961 ctcatctgta gttgatagcg acgagagttc gacgcgttca gcagtcgcaa gcgcacccag
     7021 ccacgcgaga cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca
     7081 aagccgccgc ttcccggttc gttgtattct ggcgtaccaa agttatccag ccgtttatcc
     7141 tggataatga ccggaaaatc atccacacca taatggttgg ggataggcag cgacttgctg
     7201 acttcatctt ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg
     7261 ttgggagtat tggcgtgata ccacagagta gctgcgttct ggcgaatagg cagtacgggt
     7321 gcccagtcag cgtttggcga catcatccgt gccggaccgc ccatcagcgg gcctggtacc
     7381 tgtagcccgg ccaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta
     7441 acatcgtcgc ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccag
     7501 accgacgcgc gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc
     7561 ggttgcccac ggcgagattc aagtagcggc ggaacgggta gcggttgctg ttgcccggct
     7621 gcgctggcct tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac
     7681 tgacgccgac tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag
     7741 gaaagtgttc cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt
     7801 ctgcgacttc tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag
     7861 ccagctcacg aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga
     7921 caatcaccag accgttgtgc agtcgattaa gattaatctt attcgaagtt gtagagacgc
     7981 acacgggaat aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg
     8041 gtagcaggcc gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc
     8101 gttttttgaa atgattcacg acttccgcaa tggtgccgtg agctttagtg cgattgtttc
     8161 tgtcgatcaa taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca
     8221 agctcttttt acctaccgtc accgtcggcg gttgcacaat gttcgatgct gtcaccatgt
     8281 catagttgtt ctggtggtta gcgatataga tagcattgcc gtagctttca gcatctgcag
     8341 gtttgcggca ctcaactttc aggccgaaca gtggcgcaag acggccaaac atatgcccaa
     8401 aggtggccac atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac
     8461 agactaagat gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct
     8521 ctgaaggtct gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa
     8581 agccgctctc tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag
     8641 ggaattttta tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc
     8701 cggcgttcct tgctagcggg aggaaacagc gccctccccg gcatattact cttcgctatc
     8761 accgctgctg gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg
     8821 catcaacgta ccgcggcgtc cacgttcgcc agtgactttc tgtagctctt ccggacgcag
     8881 tttaattttg cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacag
     8941 ttgcgccaga ccatcctcgc cacgtgcggc ttctgccgat ggaatgttga taatcttgtt
     9001 gcctttgccc ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc
     9061 ctgagtgatt gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg
     9121 ggcattttcc ggtaaggtga tcaaagcctt acccgcacgg ttacgcgcca ccagatcgtt
     9181 aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct
     9241 ttccatcagc atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg
     9301 ttcgccctga ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt
     9361 ggaatcaaca aacactaccg gttggttact cttacctttc accgccgctt tgaagctatc
     9421 acccgcttta taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca
     9481 gcccatctgc gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat
     9541 agctttcgct tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc
     9601 gtctgcctgc agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc
     9661 ctgcaactgg tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc
     9721 cagtttggca agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag
     9781 gccaaaccgc gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac
     9841 ttcgtcgatg ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac
     9901 tttctccaga cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc
     9961 ggagaggatt tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag
    10021 gttaatgcga tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc
    10081 catatccacg cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga
    10141 ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg
    10201 ctccagtacg cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc
    10261 ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg
    10321 aatctcggcg cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat
    10381 atccagcagc tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc
    10441 tacttcacgc aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt
    10501 gccgttaagc aaaatgtttg gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc
    10561 gtcgaagttt ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga
    10621 atatttcgac aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg
    10681 cgcgccccag ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat
    10741 caggaccatc gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc
    10801 accgacagta cgggccgatt ttttaaattt ggctctggca ttcaggccca gttcagacat
    10861 cgcatacaca atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg
    10921 gtccatgatc acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc
    10981 aaggcgctct gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa
    11041 aggggcaata ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg
    11101 tgaatagcgc cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac
    11161 aactctttgc tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca
    11221 ccactcggca tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg
    11281 gaagcctaaa gcatcaaagt agtcgactgt aagtatacgg ttcagcccag tttagctgtg
    11341 tctccaccag tgccccgcgc ctcctgccat aagtgcggct tgctcatgga tacccccgag
    11401 acggcttacg gcgcaccgga taataaattt cgcgtataca tggtattcct taccggagaa
    11461 attataagcc caacaacaat acgcctgtga cattgttcgc tcaaacttat cgcgagtgat
    11521 ttggctcaca ctccggtaaa gattccccat cacgccaacg gcacataaac atccgtttcc
    11581 agtcgctgct ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta
    11641 cgcattttct cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa
    11701 atatcgtcca gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca
    11761 ttgctcacac cataacgatt atcgggaatc ggttcgctaa cgctgccgca gatatcaaag
    11821 cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa agtctggctt
    11881 tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag
    11941 ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg
    12001 ataatgttta catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg
    12061 aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat
    12121 ttcaacagaa ttccagtctt tatcgacttc accctgaatc tcaaccgtat ctttcggcgt
    12181 caccgtcaca ccgttccagc gtttgtgatc gatatcaaca ttgatagtac cgctggcatc
    12241 tttaaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca
    12301 ggtgtcgtca cgcagggatt ttacgctttc tacagtcgtt acgctgccgt tcggcccctg
    12361 aaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct gctctgctgc
    12421 catcaccggt gcactgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt
    12481 tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt
    12541 aagggaaaaa aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag
    12601 caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct
    12661 gattggtgac ggcatcaaaa ctggccttag taaaatgggt tttagtgtcg actggtttac
    12721 acaaggtcgt cagggaaaag aggcgcttta tagcgcacct tatgatgcgg tgatcctgga
    12781 tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag agaaaggtca
    12841 gcgtgagccg gtactgaacc tgaccgcgcg tgatgcgtta gcggaacgtg tagaagggct
    12901 gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag
    12961 gctggaggct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa
    13021 cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa
    13081 accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg
    13141 caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga
    13201 agtgcatgtg catcatctgc gacgcaaact cagtagtgat tttattcgta ccgtgcatgg
    13261 tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg
    13321 ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc
    13381 tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc
    13441 aaacggttaa gtacgcccga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg
    13501 ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc
    13561 cacgacggca gaatggtcct taatgatggc gataacggag aagatattcc ctatagctat
    13621 caacgggaag gttttgctga cgggcaactg atcggtgaag acgatccttg gcgttttgtc
    13681 tggatgacct cacctgatgg caaatatcgc atcgttgttg gccaggaatg ggaataccgt
    13741 gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggctgct cgcactgccg
    13801 attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg
    13861 gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc gactggcgta
    13921 cccagcgaag tgcggccact ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg
    13981 atgatggttc gagaacgacg ctttacctcc gatgcagctc acgaactacg tagcccgtta
    14041 acagcgctga aagtgcaaac cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg
    14101 aaaaaagcac tgctccaatt acattccggg atcgatcgcg ctactcgtct ggttgatcaa
    14161 ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg
    14221 cttgaagatc tcctacaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa
    14281 attgacgtgc gactgacact caatgcccac ggcatcaaac gcaccggaca accgctattg
    14341 ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acagggcagc
    14401 gtgatagacg tcacgctgaa tgctgataat ttcatcgtga gggataacgg ccccggtgtg
    14461 acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccacccgg acaaaccgct
    14521 accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaagctgca tgacatgaat
    14581 gttgagtttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg gtaacttctc
    14641 tgtctatgag agccgttaaa acgactctca tagattttac taatagcaaa atataaaccg
    14701 tccccaaaaa agccaccaac cacaaaccag acaaataacg agcgagaata catatagcca
    14761 aaactcgaac atttcccgca taaagagttt tcttaagata agaataataa gtggcgtaag
    14821 aagaaaaaat gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa cggaaggtat
    14881 actgcaaata ttgcctcccg actcaagcat ccagtcatac ttattgaaag caaaaaaaga
    14941 atctattaac caataagtaa gcattatata tagcaatgct tcaaatattg ttttaatttt
    15001 ctgctttaac atatatcagt acgctcataa aaatccattt ataattatca acatgacacg
    15061 ttgatatttt catcttttac ttattcaaag ccatattttc aatccccaaa aaaaccgaac
    15121 caaacgtgta aattattccg gtacgagtaa gaattatgag gaatggctat cagtattgtc
    15181 attttcagaa aatatttatc ctgcatcggt gagtcagagc tagatcagac ttttgctaaa
    15241 ttcgcaaaag actttgcaca ttttgctaat ttcaccgtac cgctctgtga cgtactatag
    15301 tcgacaaacg tctcaccttg aggttaaaaa atgagcaaca tcctgattat caacggcgcg
    15361 aaaaaattcg cccactccaa tggtcaactg aacgacaccc tgaccgaagt cgcggatggc
    15421 acactgcgcg acctcgggca tgatgtccgc atcgttcgcg ccgacagcga ctacaatgtc
    15481 aaagcggaag tacaaaactt tctctgggct gatgtggtga tctggcagat gccaggctgg
    15541 tggatgggcg cgccgtggac agtgaaaaaa tatattgatg atgtattcac cgaaggtcac
    15601 gggactctgt atgccagcga tggtcgtacc cgcaaagatc cgtcgaaaaa atacggttcc
    15661 ggcggcctgg tacagggcaa aaaatatatg ctttctctaa cctggaacgc accaatggaa
    15721 gccttcaccg aaaaagatca gttcttccac ggcgttggcg ttgacggtgt gtatctgccg
    15781 ttccataaag caaaccaatt cctcggtatg gaaccgctgc cgacatttat cgctaatgac
    15841 gtgataaaaa tgcctgatgt tccccgctat actgaagaat atcgcaagca tcttgtggaa
    15901 atttttggtt aactagagct caggctttag aaggagttaa ccatgcttac cgtaatcgca
    15961 gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcgg tattggatca gtttgctaaa
    16021 atcgttccaa ccgtactgaa agaagaaggt tgccacggct atgcgccaat ggtggattgc
    16081 gctgctggcg tgagtttcca gtctatggca ccggattcta tcgtgatgat tgagcagtgg
    16141 gaaagcatcg cgcaccttga agcgcatctg caaaccccgc acatgaaggc gtacagcaaa
    16201 gccgtaaaag gcgacgtgct ggagatgaat atccgtattc tgcagccagg gatttaatcc
    16261 tgccttgttt gcccggccat cctgaccggg caatgttctt tcctttaaac ctcaatctcc
    16321 gccatgtcgc ctttctcttg caaccagttg cggcgatctt ccgagcgttt cttcgccagc
    16381 agcatatcca tcatcgcgtc agtacgctga tcgtcttcat catcgatagt caactgcacc
    16441 agacggcgag tgttcggatc aagcgtggtt tcacgcaatt gcatcgggtt catttccccc
    16501 agacctttaa aacgctggac gttcggcttg cctttcttgc gttttaattg ctcaagtacg
    16561 ccctctttct cttcttccgt cagcgcgtaa taaacctctt tcccgagatc aatacggtag
    16621 agcggtggca gtgcgacgta aacgtgaccg tgtttcacca acgcgcggaa gtgttttacg
    16681 aacaaagcgc agagcagcgt ggcaatgtgc agaccatcag agtccgcatc cgcgaggata
    16741 cagattttgc cataacgaag ctggctcaga tcgtcgctgt caggatcgat accgatcgct
    16801 accgaaatat catgcacttc ctgcgaggcc agcacttcgt cggaagaaac ttcccaggtg
    16861 ttaaggatct tacctttcag cggcatgatc gcctgatatt cacgatcgcg cgcctgcttg
    16921 gcagatccgc ctgcggagtc accttccacg aggaacagtt cggtacggtt aaggtcctgc
    16981 gcggtacaat cagccagttt gccaggcagc gccgggccgc tggtcagctt tttacgcacc
    17041 acttttttag ccgcacgcat acgacgctgg gcgctggaaa tcgccatctc tgccagcaac
    17101 tccgccgcct gaacgttctg gttcagccac aggatgaagg catctttcac cacgcccgaa
    17161 acgaatgccg cacactgacg cgaagagaga cgctctttgg tctgcccggc aaactgcgga
    17221 tcctgcattt taaccgacag aacataggcg cagcgatccc agatatcttc cgccgacagc
    17281 ttcacgccgc gcggcagaat gttgcggtat tcacagaact cacgcatcgc gtccaacagg
    17341 ccctgacgca gaccattaac atgggtaccg ccctgcatcg ttgggataag gttgacgtag
    17401 ctttcggtca gcagttcacc gccttccggc agccacagta gcgcccagtc cacagcttca
    17461 gtatcgccag cgaaattacc gataaacggt ttttctggca gcgtcggtaa accgtttacc
    17521 gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc agcgctgttc ggtgttgttg
    17581 atctcatctt taaaggtaat ctcaacgccg gggcacagta ctgctttggc cttcagcaca
    17641 tgcgtcaggc gtgaaacaga aaaacgcggg ctgtcaaaga aggtttcatc cggccagaag
    17701 tgcacgctgg taccggtatt gcgtttaccg caagtgccga caacctgtaa atcctgcacc
    17761 ttttcgccat tttcaaaggc gatgttataa acctgaccat cgcggcgcac gttaacttct
    17821 acgcgcttcg acaaggcgtt aactaccgaa atccccacgc catgcaggcc gccagagaac
    17881 tggtaatttt tgttagagaa tttaccgcct gcatgcagac ggcaaagaat cagttcaacc
    17941 gccggtactc cttcttccgg gtgaatatcc accggcatcc cgcgcccatc gtcaatcact
    18001 tctaacgact ggtcagcatg taagattaca tccacgcgtt ttgcgtgacc cgccagtgct
    18061 tcatcgacac tgttatcaat aacttcttgc cccaaatggt tagggcgagt ggtatcggta
    18121 tacatccccg gacggcggcg aaccggctca agcccggtga gtacctcaat ggcatcagcg
    18181 ttataagttt gcgtcatggt ttaagttagc aattcgagtt gatcgtcaga gatggtgcag
    18241 accaagaaaa tcgacgatcg ggttgaaata atcttcgaag cccgtgaatg cgtggttgcc
    18301 gccttctatg acagtctggc ggcaggaagc gtagtacgcc accgcctggc ggtaatccag
    18361 cacttcatct cccgtctgtt gcagcagcca gatcaaatcc ggcgcttcca gcgggtcaat
    18421 ctgcatgact ttgagatcgt aaatatggcg tgactctagc acatattgct gcccggtgta
    18481 ggggttctcg ttctgaccga gatagtccgt cagcaattca aacgggcgta ccgccgggtt
    18541 taccaccact gcgggcagca taaaacattg cgacaaccag gtggcgtaat atccccccag
    18601 tgacgaaccg acaataccca gcgaatcgcc gccatgttcc aggacaatgg attccagcag
    18661 ttctgccgcg tcggaaggat acggcggcaa ctgcggaatg atcatctcaa cgtcagggtg
    18721 atgttccgcc agccagtttt ttaacaagct cgcttttgca gagcgcggcg agctgttgaa
    18781 accgtgtaaa taaagaagcg tagacatcag tagccttctg aagcggtatc aggttggaaa
    18841 cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc catcagcatg taactcgaga
    18901 gtacgccagc cgggcgcgat agtatccagc gtaaagttgg aacagtgcgg cttaaactgc
    18961 acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc agtcgagatc cagctcctga
    19021 tgaatatgac cgcacagcaa gtatttgacg tgcggaaact tcgccagcac ggtatccagt
    19081 tcgcccgcgt tacgcagact gtgttgatcg agccaactac aacccgcagg tagcggatga
    19141 tgatgcagca gcagcaacgt atggcgttct ggcgcatcgg ccagtttacg ttccagccac
    19201 tcaagctgaa actcgctcag ctcaccgtgc ggcacgccaa acacctggct atccagcaac
    19261 aggatttgcc attgctcacc aataaacacc cgcttcgccg gggagatacc cgcatcctgt
    19321 aacgcgctgt acatcgcggg ctggaaatcg tggttgcccg gcagccagac gcagggcgca
    19381 cgaaaacttg cgatgccttc agcgaaatgc tgataggccg cagaggattg atcctgcgct
    19441 aaatcacctg tcgcgacaat caggtcgaat tcgtgctggt gtggacgaat cgcctccagc
    19501 accgcctggt agctttccca ggtgtttacc cctaacaggg cttcgtgctt ttgtgcaaac
    19561 aggtgagtat cggtaatttg taaaatcctt cctctggcct caccagccag aggaagggtt
    19621 aacaggcttt ccaaatggtg tccttaggtt tcacgacgct agtaaaccgg aatcgccatc
    19681 gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa actggttaat ttgatgcttt
    19741 tcgtcgcgtt gatgcaactt tttattagga taatcatacc gcgctttgaa gcgaaaaatc
    19801 tgctggcttg aacacacttc agccaccatc gcgtcatgat acagacgcac cgtcattgac
    19861 ggaaggctcc agtaactgat cgcgggcgca gtctgttcta ttgtcaccag ggtagtgtat
    19921 cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg ccacctgata gcttacagtt
    19981 tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt gtgaaaagtt catctcgcac
    20041 aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca tttatgccca ctcatttttt
    20101 aacgcttgat gatgcagctg cagccattgc aaagcgatga ccgacgctgc gttgtcgatt
    20161 ttcccctctt ctacccactg gtatgcctgt tcccggctta ccacatgaac gcgaatatct
    20221 tcgttttcat cagccagacc atgaataccg cttgcggtcg tggcgtccac ttcgcccacc
    20281 ataattgacg aacgctcact ggtgcccccc gggcttgcca ggaaacttaa caccggtttg
    20341 gtccgtttga ctatcagtcc cgcctcttca atcgcttcgc gacgggcaac atcttccaca
    20401 ctttcaccct cttcaatcat cccggcaacc atctccagta gccagggggt ttcgctggtg
    20461 tcgtacgcgg caatccgaat ctgctcaatc agcacaactt catcacgcac tgggtcaaag
    20521 ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc gccgtacctc atgactcatt
    20581 tgcccgttga atagacgatg acgaaatcta taaagatcta atgaaaaaaa gccgcgataa
    20641 agtgtttctc gtgcaataat ttctacatcg tttttgccaa atgtaacggg caggttgtct
    20701 ggcttaagca ttgttaatgt cctggcacta atagtgaatt aaatgtgaat ttcagcgacg
    20761 tttgactgcc gtttgagcag tcatatgtta aattgaggca cattaacgcc ctatggcacg
    20821 taacgccaac cttttgcggt agcggcttct gctagaatcc gcaataattt tacagtttga
    20881 tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa gaaattgctc cccattctta
    20941 tcggcctgag cctttctggg ttcagttcgt tgagccaggc cgagaacctg atgcaagttt
    21001 atcagcaagc acgccttagt aacccggaat tgcgtaagtc tgccgccgat cgtgatgctg
    21061 cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc acagctaggt ttaggtgcag
    21121 attacaccta tagcaacggc taccgcgacg cgaacggcat caactcgaac gcgaccagtg
    21181 cgtccctgca gttaactcaa tccatttttg atatgtcgaa atggcgtgcg ttaacgctgc
    21241 aggaaaaagc agcagggatt caggacgtca catatcagac cgatcagcaa accttgatcc
    21301 tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat tgacgttctt tcctatacac
    21361 aggcacaaaa agaagcgatc taccgtcaat tagatcaaac cacccaacgt tttaacgtgg
    21421 gcctggtagc gatcaccgac gtgcagaacg cccgcgcgca gtacgatacc gtgctggcga
    21481 acgaagtgac cgcacgtaat aaccttgata acgcggtaga gcagctgcgc cagatcaccg
    21541 gtaactacta tccggaactg gcggcgctga atgtcgaaaa ctttaaaacc gacaaaccac
    21601 agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa cctgtcgctg ttacaggcac
    21661 gcttgagcca ggacctggcg cgcgagcaaa ttcgccaggc gcaggatggt cacttaccga
    21721 ctctggattt aacggcttct accgggattt ctgacacctc ttatagcggt tcgaaaaccc
    21781 gtggtgccgc tggtacccag tatgacgata gcaatatggg ccagaacaaa gttggcctga
    21841 gcttctcgct gccgatttat cagggcggaa tggttaactc gcaggtgaaa caggcacagt
    21901 acaactttgt tggtgccagt gagcaactgg aaagcgcaca tcgtagcgtc gtgcaaaccg
    21961 tacgttcctc cttcaacaac attaatgctt ctatcagtag tattaacgcc tacaaacaag
    22021 ccgtagtttc cgcgcaaagc tcattagacg cgatggaagc gggctactcg gtcggtacgc
    22081 gtaccattgt tgatgtgttg gatgcgacca ccacgctgta caacgccaag caagagctgg
    22141 cgaatgcgcg ttataactac ctgattaatc agctgaatat taagtcagcc ctgggtacgt
    22201 tgaacgagca ggatttgctg gcactgaaca atgcgctgag caaaccggtt tccactaatc
    22261 cggaaaacgt tgccccgcaa acgccggaac agaatgctat tgctgatggt tatgcgcctg
    22321 atagcccggc acccgtcgtt cagcaaacat ccgcacgcac taccaccagt aacggtcata
    22381 accctttccg taactgatga cgacgacggg gcttcggccc cgtctgaacg taaggcaacg
    22441 taaagatacg ggttatctgc cgcattcttc ccccttctcg cttcaatttc gaccagccat
    22501 cctctattct gatgggtatt taccactggt cccggaagac aaaaatgaaa cggacaaaat
    22561 ccatacgcca cgcatcgttc cgcaaaaact ggagcgcacg ccatctgaca ccagtcgctc
    22621 tcgcggttgc cactgttttt atgctggctg gctgtgaaaa gagtgatgaa acagtgtctc
    22681 tctatcaaaa tgctgacgac tgttcagctg caaacccagg caaaagcgcc gaatgtacca
    22741 ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc gccgaaatac gccacccgtg
    22801 aagactgtgt tgctgaattt ggtgaaggtc agtgtcagca agcaccagcc caggctggca
    22861 tggcaccaga aaaccaggcg caggctcagc aatccagcgg gagtttctgg atgccgctga
    22921 tggccggtta catgatgggg cgtctgatgg gcggcggcgc gggatttgca cagcagccgc
    22981 tgttctcctc gaaaaacccg gccagtccgg cttacggtaa atataccgac gcgacgggta
    23041 aaaactatgg cgcagcccag ccaggccgca ccatgaccgt accgaagacg gcaatggcac
    23101 caaaaccggc gaccaccact accgttaccc gtggcggttt tggtgaatct gttgccaaac
    23161 aaagcactat gcagcgtagc gcaaccggca cctcttctcg ttcaatgggt ggctgatacc
    23221 gatggaaaga gtcagtatta ccgagcgccc ggactggcgt gagaaagccc acgaatacgg
    23281 tttcaatttt cacaccatgt acggcgagcc gtactggtgt gaagatgctt actacaagtt
    23341 gaccctcgcc caggttgaaa agctggaaga agtcaccgcc gaactgcacc agatgtgcct
    23401 gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg accaaattcc gcataccaaa
    23461 acacacctgg agttttgtgc gtcaatcatg gctgactcac cagccatcgc tttattcgcg
    23521 tcttgatctg gcgtgggatg gcactggcga acctaaactt ctggaaaata acgccgatac
    23581 gccaacgtca ctatacgagg cggcgttctt tcagtggatc tggctggaag atcagcttaa
    23641 cgccggtaac ttgccggagg gcagcgacca gtttaacagt ctgcaagaaa aactgatcga
    23701 tcgcttcgtt gagctgcgtg aacagtgtgg cttccagttg ctgcatctca cctgctgtcg
    23761 cgacacggtg gaagatcgcg gaaccattca gtatttgcag gactgcgcaa ccgaagctga
    23821 aatcgccacc gagttcctct acatcgacga gataggttta ggcgaaaaag gccagttcac
    23881 ggatttacag gatcaggtga tttctaacct gttcaagctt tatccgtggg aatttatgct
    23941 gcgcgaaatg ttctccacca agctggaaga tgctggcgta cgctggctgg aaccggcgtg
    24001 gaagagtatt atctccaaca aggcacttct accgctactg tgggagatgt tcccgaatca
    24061 cccgaacctg ctgcccgctt attttgcgga agatgatcat ccgcaaatgg aaaaatatgt
    24121 ggttaaaccg atcttctccc gtgaaggcgc aaacgtgtcg atcattgaga acggcaaaac
    24181 cattgaagca gcggaaggtc cgtatggcga agaagggatg attgttcagc aattccaccc
    24241 gttaccgaaa ttcggcgaca gctatatgct gattggtagc tggctggtga acgatcaacc
    24301 tgccggaatt ggcattcgtg aagaccgtgc gttgatcacc caggatatgt ctcggtttta
    24361 tccacatatt tttgttgaat aagccacgat accggatggc actcgccatc cggtaattgt
    24421 tagcctatct gcaccgacag catacttaaa ctgcccattt ctataccatc gaccggaatg
    24481 gtaattggct cctgcccatc ccacacacct aacacataca acaacggcag atagtgctct
    24541 ggcgttgggt tcgataacgc gccaccttca tggtcgagat aattcaccag aggatgctgc
    24601 tctaccggcc cttgccacgt cagattcgct ttcacatact cattaaacga cgtcgcccac
    24661 ggatacggtg aactatcacc gtgccacttc actgtgcgca ggttatgcac cacgttaccg
    24721 ctggcgacca acattattcc ttcatcacgc aacgccgcga gtttgcgccc catttcgaaa
    24781 tgccaggcgg caggtttgct actgtcgata ctcaactgca ccatagggat atcggcgtca
    24841 ggatacatct taatcagcac gccccacgag ccgtggtcaa agccccaggc ttctttatcc
    24901 agcgtcaccg ggatcggcgc taacagctca accagacgct gtgccagcgc aggcgaaccc
    24961 ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg
    25021 ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa accagtgagc cgaaaccacc
    25081 acaatcgctt gcgggcgtgg caatgtcatc cccaacttct gccagctgcg ggtatacaaa
    25141 ttatcttcca gcacgttcat cggactaccg tgacctaaaa acaatgctgg catacgtgtt
    25201 gaagacatga tgatatcctt aactaaaggt gtcattttga tatcctcaca atacgcttgt
    25261 tcggcggagt aagaacccgg ataacaatga tgatgatcat cagttatttt gacgatctgc
    25321 ctgaaggtga agatttataa ggagttgtcg atgtcagtac ctctcattct gaccatactg
    25381 gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc tcggacaaaa accctcgaac
    25441 cgcttactgg cgttttcgct aggttttgcg gcggggatca tgttgctcat ctcattaatg
    25501 gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc cggtgttggg ttatggaatg
    25561 tttatcttcg gtctgcttgg ctactttggc ctggaccgca tgttgccgca tgcgcatccg
    25621 caggatttaa tgcaaaaatc ggtgcagctg ttgccaaaat cgatcaagcg cacagccatt
    25681 ctgctcactc tcggcatcag tctgcataac ttcccggaag ggattgccac ctttgtcacg
    25741 gcgagcagca acctggagct gggatttggc atcgcactgg ccgtcgcgtt gcacaatatc
    25801 cctgaaggtc tggcagtggc aggcccggtt tatgcggcaa cggggtctaa acgcaccgcg
    25861 attctgtggg cggggatttc tggactagca gaaattcttg gtggtgtgct ggcgtggtta
    25921 atcctcggta gcatgatttc cccggtggtc atggcggcaa tcatggcggc ggttgcagga
    25981 attatggtgg cgctctcggt tgatgaatta atgccgctcg ccaaagagat tgaccctaat
    26041 aataacccca gctatggcgt actatgtgga atgtcagtga tgggattcag tttagtgctg
    26101 ctacaaacgg cgggaattgg ttaaaaacaa catcagaagt tgtccagcac atggacaact
    26161 tctgccagct tcacgaaagc cttattctac cttttcggct gtctcttctc tcgtactgtt
    26221 taagtatttg ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg cccgacaata
    26281 acgtttcaaa tgcaggaaat aacccaccac aaccagagca atcaatacaa tccagtacca
    26341 ggcgataaac atccttttac ccttaatatt taacaatact aaacatttag cgcataaatt
    26401 tcacatatcc tttttcggat atattcatat ggtcgcataa taacggaaca attatatgat
    26461 tttgatcata atgaaaaaga atgcctgata ttaatgaaat tttctcatag aaagagaagc
    26521 ggaattaaca tcttgcatag cagggcaaaa taaaaaccga agccagctac aacttcggtt
    26581 tcttgattaa ggcagtaatt aagcagcggt tttcagctgg ctttacgctc atgtgcctga
    26641 cggtatgcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt ggcaaactca
    26701 atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca cagcacacca
    26761 gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg cttcagtatg accgccacgc
    26821 gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt ggcccgggcg attcagatca
    26881 gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taatacgatc agcggcagaa
    26941 acaccggtag tcacaccttc agccgcttca atggtcacgg taaaaccggt gccataggcg
    27001 ctggtgttat tttctaccat cattggcaga tcaagttgtt tacggcgatc ttcagtgatg
    27061 cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc aacggtcatg
    27121 gtttctgccg ggaagatcat atcgccttcg ttttcacggt cttcatcatc aagcaccatt
    27181 acaccgcgtc cttcacgcag cgcagccagt gcattttcaa cacgttcgaa aggcgtacca
    27241 aaagaggaaa gtagcgtctg attcatggta aaaaaaacct cactaaaatt atggttacca
    27301 gaatcagggc agtcttagga gtggcggcat atagccaaaa taacgtgagc gggtccatgc
    27361 ccgacagaat cgttactctc tcccatccgg actctaaccg tcggccccgg aattacaccg
    27421 gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact gagttgattt
    27481 accgccggtg gggaatttcg ccccgccctg agaataagcg gattcactat aactctaatg
    27541 attagcggca gcaacgcata gcttcacata attctggttt atgacttacc cttatcgcac
    27601 tacaatggta ctcaacacct atcagtacag ggaaactaca atgattgacc cgaaaaaaat
    27661 tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga
    27721 agatgtggag aaaaaaatcc gccaaaccct acaagctcag ctgacgcgcc tcgaccttgt
    27781 aagccgtgaa gagttcgacg tccaaacgca agtcctgtta cggactcgtg agaaactggc
    27841 gctgcttgaa caaagaatca gcgaactgga aaatcgcagt actgagatca aaaagcagcc
    27901 agatccagag actctccccc caacgctgta aacatttgtc tttaatgcac tgtaaattat
    27961 gaattaatcc tctaatttac agtgttttct attacattat cgcttaatcc cattcagagt
    28021 atcatcacaa aaatgaatga ttgcaattga aaatataatt gttatgtatt ctttaagttt
    28081 tacttaatcg at
//
LOCUS       DADWTH010000047        27687 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.047, whole genome shotgun sequence.
ACCESSION   DADWTH010000047 DADWTH010000000
VERSION     DADWTH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.047"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..677)
                     /gene="ycjM"
                     /locus_tag="JNY02_002157"
     CDS             complement(<1..677)
                     /gene="ycjM"
                     /locus_tag="JNY02_002157"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBB9433688.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYV"
     gene            complement(889..1203)
                     /gene="pspE"
                     /locus_tag="JNY02_002158"
     CDS             complement(889..1203)
                     /gene="pspE"
                     /locus_tag="JNY02_002158"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBB9433689.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="JNY02_002159"
     CDS             complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="JNY02_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBB9433690.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="JNY02_002160"
     CDS             complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="JNY02_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBB9433691.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="JNY02_002161"
     CDS             complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="JNY02_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBB9433692.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="JNY02_002162"
     CDS             complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="JNY02_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBB9433693.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2980..3957
                     /gene="pspF"
                     /locus_tag="JNY02_002163"
     CDS             2980..3957
                     /gene="pspF"
                     /locus_tag="JNY02_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBB9433694.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLQDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="JNY02_002164"
     CDS             complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="JNY02_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBB9433695.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5380..6660)
                     /gene="puuB"
                     /locus_tag="JNY02_002165"
     CDS             complement(5380..6660)
                     /gene="puuB"
                     /locus_tag="JNY02_002165"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBB9433696.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="JNY02_002166"
     CDS             complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="JNY02_002166"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBB9433697.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="JNY02_002167"
     CDS             complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="JNY02_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBB9433698.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="JNY02_002168"
     CDS             complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="JNY02_002168"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBB9433699.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9859..11276
                     /gene="puuA"
                     /locus_tag="JNY02_002169"
                     /pseudo
     CDS             9859..11276
                     /gene="puuA"
                     /locus_tag="JNY02_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
     gene            11425..11589
                     /locus_tag="JNY02_002170"
     CDS             11425..11589
                     /locus_tag="JNY02_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433700.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11579..12964
                     /gene="puuP"
                     /locus_tag="JNY02_002171"
     CDS             11579..12964
                     /gene="puuP"
                     /locus_tag="JNY02_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBB9433701.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVHQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13098..13343
                     /locus_tag="JNY02_002172"
     CDS             13098..13343
                     /locus_tag="JNY02_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBB9433702.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTHLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13656..15299
                     /gene="sapA"
                     /locus_tag="JNY02_002173"
     CDS             13656..15299
                     /gene="sapA"
                     /locus_tag="JNY02_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBB9433703.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15296..16261
                     /gene="sapB"
                     /locus_tag="JNY02_002174"
     CDS             15296..16261
                     /gene="sapB"
                     /locus_tag="JNY02_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBB9433704.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16248..17138
                     /gene="sapC"
                     /locus_tag="JNY02_002175"
     CDS             16248..17138
                     /gene="sapC"
                     /locus_tag="JNY02_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBB9433705.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17138..18130
                     /gene="sapD"
                     /locus_tag="JNY02_002176"
     CDS             17138..18130
                     /gene="sapD"
                     /locus_tag="JNY02_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBB9433706.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18132..18938
                     /gene="sapF"
                     /locus_tag="JNY02_002177"
     CDS             18132..18938
                     /gene="sapF"
                     /locus_tag="JNY02_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBB9433707.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19006..19359
                     /locus_tag="JNY02_002178"
     CDS             19006..19359
                     /locus_tag="JNY02_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBB9433708.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19727..20515
                     /gene="fabI"
                     /locus_tag="JNY02_002179"
     CDS             19727..20515
                     /gene="fabI"
                     /locus_tag="JNY02_002179"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBB9433709.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20659..21786
                     /locus_tag="JNY02_002180"
     CDS             20659..21786
                     /locus_tag="JNY02_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBB9433710.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21854..23788
                     /locus_tag="JNY02_002181"
     CDS             21854..23788
                     /locus_tag="JNY02_002181"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBB9433711.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23866..23968)
                     /locus_tag="JNY02_002182"
                     /pseudo
     CDS             complement(23866..23968)
                     /locus_tag="JNY02_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24022..25996
                     /gene="pdeR"
                     /locus_tag="JNY02_002183"
                     /pseudo
     CDS             24022..25996
                     /gene="pdeR"
                     /locus_tag="JNY02_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26144..26317
                     /locus_tag="JNY02_002184"
     CDS             26144..26317
                     /locus_tag="JNY02_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433712.1"
                     /translation="MSEFDTQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26407..27156
                     /locus_tag="JNY02_002185"
     CDS             26407..27156
                     /locus_tag="JNY02_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB9433713.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27425..27643
                     /gene="osmB"
                     /locus_tag="JNY02_002186"
     CDS             27425..27643
                     /gene="osmB"
                     /locus_tag="JNY02_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBB9433714.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         6438 a         7149 c         7117 g         6983 t
ORIGIN      
        1 acatactcag cacctttctc aaggtaacag agtaaaacat ccaccatcgc cagcaacact
       61 tcagggctac ggtaattcag gtcaatttga tcgtcactaa aggtggtcca taaatggcgc
      121 gtggaattat catccatttg aaacggcgtt aatagcggta acgcacgcgg acgggtgacg
      181 gcactgagat cggttttcgg atcaaccgca ataaagaaat cttcaaaacc cggaagctgt
      241 tgtaaatagt ttttaaacca ttcacttttt gcagacatat ggttgcagac aaaatcaaac
      301 attaaatgac tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg
      361 acctgatgat aatcaattac cgaaaatcca tcatcagatg accatgggta aaaaggtaat
      421 aaatgaacat gtgaaaaaat gctttgcagc cattgatggt agaactgatt aaatatgggt
      481 agtggtttta aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt
      541 tcatcccagt gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt
      601 acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatcc
      661 gtaattttct gtttcatgtt gcctttccgt ggtagcggtc ccatgaggcg agagactact
      721 ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt
      781 taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatagggat gtaaataagg
      841 tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac
      901 cttcggcatt gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc
      961 gctaaggatc tcttttgcct gcccggactg gcgtccggca ttgcaataca ctttcacggt
     1021 gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt
     1081 gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg
     1141 ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa
     1201 catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa
     1261 tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc agcccgactt
     1321 aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc aactgatttt
     1381 atcgcccagc ccgccacacc cgccgggcca tagcgcagtg cggtaagaag taccagcttg
     1441 cctgctaatt tgaaaccaag cttcaccttt tgcccggcct gttgccagcg agtattcata
     1501 actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac
     1561 gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca
     1621 attcgctgct ggaaggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg
     1681 caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca
     1741 gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca atcccggcgc
     1801 agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc
     1861 ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt ccagcgcctg
     1921 aatacgttcg cgcatccgtt ttgcttcatc aaccagttgc gctaatcgct gctgctcact
     1981 ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa
     2041 aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca taagtactcc
     2101 ttagacgatg agcggcggcg cgttcggacg ccgcctggat gttattattg attgtcttgc
     2161 ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc tttcagttcg
     2221 gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc
     2281 atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca
     2341 ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatgacg taacatcaat
     2401 gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcgccaat ctctttcttc
     2461 atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagggactt aatcagatcg
     2521 gtcagtttct gtttttcaat taacgctgca cgtgccagat cctctctctc tttcagcagc
     2581 gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga
     2641 gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc aaccagtgta
     2701 tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc tttctctaac
     2761 agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg
     2821 tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca
     2881 attttttatc tttttgattt ataaagatct gattgaagaa tcaacagcaa catgccagga
     2941 tgaattagcg aattacacta acaagtggcg aatttcatca tggccgaata caaagataat
     3001 ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg
     3061 ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagtt gattgccagc
     3121 cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg
     3181 ttaaatgaaa atctgcagga ttccgaactg tttggtcacg aagcgggggc gtttaccggt
     3241 gcgcaaaaac gtcatcctgg gagatttgaa cgtgccgacg gcggtacgct atttcttgat
     3301 gaactcgcta cggcaccgat gatggtgcag gaaaaattat tgcgtgtgat tgagtacggt
     3361 gaactggagc gcgttggcgg tagccagccg ttgcaggtga atgtgcggtt ggtatgcgcg
     3421 acgaatgccg atctcccggc gatggtcaat gaaggtactt ttcgcgctga cctgctcgac
     3481 cggctggctt tcgatgtagt gcaactgccg cccctgcgcg agcgcgaaag cgacattatg
     3541 ctgatggcag aacactttgc cattcagatg tgtcgggaaa tcaagctgcc tctgttcccg
     3601 gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg aaatattcgt
     3661 gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga ttatccgctt
     3721 gatgacatca ttattgatcc ctttaaacgg cgtccacctg aagaagccat cgccgtttca
     3781 gaaaatacct cgcttccgac gctgccgctg gatttacgcg agtttcagat gcagcaggaa
     3841 aaagagttgc tgcaactcag tttgcaacag gggaaatata accagaagcg cgcggctgaa
     3901 ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca gatttagcga
     3961 agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat acataatcaa
     4021 tacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt gggctattaa
     4081 tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc cgggatggtc
     4141 agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag gagcagcccc
     4201 tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc
     4261 ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc aatggccgga
     4321 acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg attcgcgcgt
     4381 tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc caccgccagc
     4441 gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat aatattcgcg
     4501 ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat cgtcattaag
     4561 tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc aaagccgctt
     4621 tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg aatggcggca
     4681 accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc gaaaataatg
     4741 gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc gtcgagggag
     4801 tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac cgaaccaggg
     4861 aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat ggtcatatat
     4921 gtacgaccgt ggaagccacc gctaaacgca atcacgccag gtcgtccggt atgggcgcga
     4981 gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc ggttttagcc
     5041 tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac
     5101 ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc
     5161 agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat
     5221 tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa gaagttacac
     5281 atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc attgttgctc
     5341 atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca gacgatcgcg
     5401 caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg
     5461 gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac cgcgcagcag
     5521 ttcagcaatc aaacgcccgg caagatgggt acaggtgacg ccgtggccgc tgtaaccctg
     5581 catgtaatag atgttggtat caaggcggcc gaactgcggc atgcgcgaca aggtcagcag
     5641 gaagttgccc gtccagcggt aatcaatttt cacgcccttc aactgaggga aggtttttag
     5701 tagtttcggt accacaagac gctcaacgtc atccgggttg cgtgcaccat acactacacc
     5761 cccgccgtac agcaggcggt tgtcggcggt aagacggtag taatcaagca gatagttaca
     5821 gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg acagccgttc
     5881 ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gctagttccg gctctacttt
     5941 atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct gaccattggc
     6001 agtccgaacc acggctggcg tggtgtgctg gatctgcgtc acggcggaaa gctcatacac
     6061 gcgcccgccg ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta gcgggtgaat
     6121 atgcccaccg ctgtgatcca gcagcgcacc agtgtagcga tcgctggcga cctcgcggcg
     6181 aatagcgttg gcgtccagca gctccagctg tttattgccg tagcgttccc agttctcttt
     6241 ctgctcttcg agtgtggcga gttgtttatc attcatcgcc acaaacagac cgccggggcg
     6301 gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcgc caccctcgaa
     6361 catcatgctg ccgagcatac gggcggtgtc catgccgtag cttttttcga tcacgtcgat
     6421 gtcgcggctg taggagttca caagctgccc gccattgcgc ccgcttgcgc caaagccgat
     6481 gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca gcgcggagga
     6541 gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact cattcagtgt
     6601 gtcgaatggt gcatatttat tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat
     6661 ttcaggcctc caggcttatc cagatggttt tcagttcagt gaatttttca agggcatgca
     6721 gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg gtcatatcgc
     6781 cgtcgttgta gttattgacg aagacggaac cggctttcag gcgtcggctc atgcggtgcg
     6841 cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg tcgttggcaa
     6901 gctgcagcgc ctgatcttct gatgtgaaac gggtgaccac cagcaccgga ccgaaaatct
     6961 cttcgcggct taaggacgca ttcgggtcca catccacgaa gatggtcggg ccgatggccg
     7021 ctgccagccc ggcgttgcga ccatccagca gcagttgccc cttgccttcg ccttcacgaa
     7081 taaaggtgtg taccgagtca gcgtgagcgc tgtcgattaa ggtgcccatg gtggttgcgg
     7141 gatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg gctaagaatt
     7201 catcggcgat gctctcttcc agtaacaaac gcgttccggc gatgcacacc tggccctggt
     7261 tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga caatcggcga
     7321 aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac acgtttcatg ttgctatcac
     7381 ccgcgtcttt cagcagttgt ttacccgtac gcgtggagcc ggtaaaggcg atggcatcga
     7441 tatcgttatg acgcgaaagc gcctgccccg cttcatgacc aaaacccgtc accacgttca
     7501 acacaccatc cggcaagcct gcttctttcg ccagccccgc gagacgaatc gcactgagcg
     7561 gtgatttttc agacggtttt agaatcacgc tgtttcccgc cgccagcgcc gggccgagtt
     7621 tccagcaagt cagcaacagc gggaagttcc acggcacgat ggcggcaatc acgccgaccg
     7681 gttcacgcac gatcatcgcc agctcatggc tactggtggt cgccacttcg ccatacactt
     7741 tgtcgatcgc ttcggcgtac cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca
     7801 gactgtgacg aatcggtttg ccggtgtcga gagtttccag cagtgccagc tcttcggcgt
     7861 gggcttccat taaatcggcg agtttattca gtaccgcttt acgtttagcc ggagaagaga
     7921 gtgaccagtc gccgcgttca aatacgccgc gagctgcgct caccgcacgg tcgatatcga
     7981 cgctcttgcc gcgggcaatt ttcgccagcg gtgcctgggt gaccggatca acggtttcaa
     8041 aggtttcatt ttccgccgca gcagtatatt caccgttaat aaataagcgg ttttcaatgg
     8101 cgagacttaa cgctttatcc tgccagtaag ccagatgatg aaaattcatt atgactcctg
     8161 tttcacgtct atcagatata tgcagtttgt gaacgtcagg gcgcagagat ccccgggctg
     8221 catgcccgtt tatttctggc gacattcttt atggattgcc gggcgcattg cgcttacccg
     8281 gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa ttcggcaaat
     8341 acctgccgac gtattactga aactgtgcgg gatgccggta ttaatggcat aactttgccc
     8401 cgcgacgagg tggtaatcct gaccattaat cgtcagaaca atttcacctt ccagtactgt
     8461 gcctatttcc tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtacgt
     8521 ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac
     8581 accctgactg cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg gctcatcagg
     8641 tttttccggc tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg
     8701 cagcgtactg atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt
     8761 cagcccggag agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga
     8821 caagcgtttt cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt
     8881 ggtcagagtc gctgtttttc agcgatatgg tgctgccaag cggtgataaa gccctcgaac
     8941 aatatacgcg aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgcccagc
     9001 gcaaaaggat gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca
     9061 acacgcaacc gtgggctaac gaccttcgcc ccttgtccat gtagagagtt tacccaaaag
     9121 ttgctacatt caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc
     9181 gacggtgcat attgctgttc caccggcagt tcgggatctt cccgatgctc cagcaattca
     9241 ggctgttcgc acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcttgtaaa
     9301 ccccggcaga tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat tatcgccatg
     9361 ctcagaagat cacgcccggg atcggcgtca ggctcatcgc cgttttcacc atataggtgc
     9421 ggctgcacat tgctgggact actaggaaga taaatgccat cgagtttcgg caaaagttgt
     9481 tcaagtaacg acggttccgc cagcgcatgt ggtagcgcaa ttggcaagcc gcctgcgtgg
     9541 atgatggcat tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc
     9601 ctgcacatta cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca
     9661 aagtggacta aattatcgcc attactgcgc tttaaatgac ctttatgttc aatatttttt
     9721 caatctagca gtggattttc atttttgcaa actaaattta acatttgaca aacatttggt
     9781 ttgcatacag attcgaatga tggtcattat attttacgct ttgataacga gcggaaaaca
     9841 aaccaaaggc gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag
     9901 tcagaagaga ggcggggtag cgcctttacg caggaagtga aacgctacct ggagcgctac
     9961 ccgaatacgc aatatgttga tgttttactt actgatttga atggttgctt ccgtggaaaa
    10021 cgtatccctg tttcaagcct gaagaagctc gagaaagggt gttatttccc ggcctcggta
    10081 tttgcaatgg atattctggg caacgtagta gaagaagcag gtctgggtca ggaaatgggc
    10141 gagccggatc gtacctgtgt tcctgttctc ggttccttaa ctccttctgc cgccgatcca
    10201 gagtttatcg atcagatgct cctgaccatg gtcgatgaag atggcgctcc ctttgacgtt
    10261 gagccgcgga acgttcttaa ccgcctgtgg cagcagctgc gccagcgcgg attgttcccg
    10321 gttgtagcgg tagagctgga gttctattta ctggatcgcc agcgcgacgc tgaaggggat
    10381 ctgcaaccgc cctgcgcgcc tggcaccgat gaccgcaata cacaaagcca ggtttactcc
    10441 gttgataacc tcaaccactt cgccgacgtg ctcaatgata ttgatgaact ggcgcagttg
    10501 cagctgattc cggcagatgg cgcggtcgct gaggcctcgc cgggtcagtt tgaaatcaac
    10561 ctttaccata ctgataacgt gctggaagcc tgcgatgatg cgctggcact aaaacgcctg
    10621 gtgcgtctga tggcagaaaa gcataagatg cacgccactt ttatggcgaa gccgtatgaa
    10681 gagcacgcgg gcagcgggat gcatatccat atcagtatgc aaaataatcg tggcgagaat
    10741 gtgctttctg acacagaagg cgaagattcg ccgctgctga aaaagatgct cgccgggatg
    10801 attgacctga tgccgtcgtc gatggcgttg ctggcaccaa acgtgaactc gtatcgccgc
    10861 ttccagccgg gaatgtatgt gccgacgcag gcgtcgtggg gccataacaa ccgcaccgtc
    10921 gccctgcgta ttccgtgcgg tgaccgtcat aatcaccgcg tggaatatca cgtggcgggt
    10981 gccgatgcca acccatatct ggtgatggca gcgatttttg ccggtatttt gcatggcctt
    11041 gataacgagc tgccgttgca ggaagaagtc gaaggcaacg ggctggaaca ggaaggctta
    11101 cccttcccga ttcgccagag cgatgcctgg gtgagtttat cgagaatgat cacctgcgcc
    11161 gctatttagg cgaacggttt tgccatgtgt atcacgcctg taaaaatgat gagctgttgc
    11221 agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac gcgtaattcc
    11281 ccttgcgggg ttgtttcatc ggcctgagcg gagccaggcc tgacatttgc tggtaatcca
    11341 gacatttccc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg ttaacgatta
    11401 agactgtgac aaggaaatga gatgatgccg atgattaaat ccccgcatgg cgagggaggc
    11461 tgcgtatgcg cccctcctgc tactgactgg actccgcccc ctttgcttcc cttgctaaac
    11521 aggtttgatt tcaggtcgac acgaccgcaa accttattac gcaggggagg cagcaattat
    11581 ggctattaat tcaccactga atattgctgc gcaacccggc aaaacccgtc tgcgaaaatc
    11641 actgaaattg tggcaggtgg taatgatggg tctggcctat ctcacgccga tgaccgtatt
    11701 tgatactttt ggtattgtct ccggcattag cgacggtcac gttccggcgt cctatttgct
    11761 ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc aaactggttc accagtttcc
    11821 ggaggccggt tcggcctata cctacgcgca aaagtcgatt aacccgcacg tcggatttat
    11881 ggtcggctgg tcatcactgc tggattatct ctttttgcca atgatcaacg ttttgttggc
    11941 gaaaatctat ctctccgccc tcttcccgga agtgccgccg tgggtgtggg tggtaacctt
    12001 cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac ctggtcgcta acttcaatac
    12061 cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt atcttcctgg tggttcaggg
    12121 actgcataaa ggagaaggcg ttggcaccgt ctggtcactt cagccgttta tcagcgagaa
    12181 cgcgcacctg atcccgatta ttaccggtgc gacgattgtc tgtttctcgt tcctcggttt
    12241 cgatgcggtg accacgcttt cggaagagac gccggacgcc gcacgcgtga tcccgaaagc
    12301 catcttcctg acggcggtat acggcggcgt tatctttatc gcggcgtcgt tctttatgca
    12361 gctgttcttt cccgatatca gccgctttaa agacccggac gccgcactgc ctgaaattgc
    12421 gctgtacgtc ggcggcaagc tctttcagtc gattttcctc tgcaccacgt ttgtgaacac
    12481 attagcgtct ggcctggctt cacatgccag cgtgtcgcgt ctgctgtatg tgatggggcg
    12541 tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac ccaaaatggc ggactccggc
    12601 actcaacgtc attatggtcg ggattgtcgc gctgtcggcg ctgttctttg atttagtcac
    12661 cgcgacagca ctgattaact tcggtgctct ggtggcgttt accttcgtga atctgtcggt
    12721 gtttaatcat ttctggcggc gtaaaggaat gaataaaagc tggaaggatc acttccacta
    12781 tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg ctgtgggtta acctagagtc
    12841 aacgtcactg acactcggtc tggtatgggc ttcgttaggc ggcgcatatt tgtggtatct
    12901 gatccgccgc tatcgcaaag tgccgctgta cgaaggtgac agaacgccgg tgagcgaaac
    12961 gtaatagccg gatgcgcggc gcattatcct gcctgagggt aacgcgccgc gccattaact
    13021 ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc ttagcgcatt
    13081 aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat attgccgatg
    13141 agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg ctggcccact
    13201 acttccagtt actgctcacc catttgatga acaatgaaga gatcagcgaa gaagcccaac
    13261 atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc gcagagtttt
    13321 tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt atcgttgtcc
    13381 ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg tgctttaatg
    13441 ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt tatagttata
    13501 gagaaatcac gggtacgaat gttgaataga tggcgacgcc acctgttcaa cgaccaacca
    13561 cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat taaatattag
    13621 taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta tcgtctcttt
    13681 tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc cccccgcatg
    13741 ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac acctttaacc
    13801 catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt tatgatcgac
    13861 tgctggatgt cgatccctat acctatcgcc tgatgccgga acttgccgaa agctgggaag
    13921 tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg tttcaaaaaa
    13981 ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt accttccagc
    14041 gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc ccctacttcg
    14101 acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat cataccgttg
    14161 agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc cattatgctt
    14221 cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag gagcaactcg
    14281 accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc gggcaattta
    14341 ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg caggtagtgg
    14401 tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg gaatgcgacg
    14461 ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg cgcttgcgtt
    14521 taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc aaaccgccgc
    14581 taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag cgcctgatgc
    14641 aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc gcctcgtggg
    14701 cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc gaacagttga
    14761 aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt tcgcaggcgt
    14821 ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg caggttggcg
    14881 taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg atggatatga
    14941 gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac agtttcttcc
    15001 gtccgttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac tggtgcgatc
    15061 cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggca gcgcgtattg
    15121 aagcctatga cgaagcgcag agtattctgg cgcaggaatt acctattctg ccgctggcgt
    15181 cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt agcccgtttg
    15241 gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa aaaccatgat
    15301 tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc tgaccttcgt
    15361 tggcttcagc ttgagctatt tcactccaca cgcgccgtta caaggcgcgt cactgtggaa
    15421 tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt ccagcattaa
    15481 cggtcaacca attgccgaac agttaaaaga ggtcttcccg gcgacgatag aactgtgcat
    15541 cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg ccgggattac
    15601 gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct tttcaatccc
    15661 ggtgttctgg ctggcgcttc tgttgacact gttttgttca ctcacgctag gttggttgcc
    15721 cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt ttgcgttgat
    15781 tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg caatccgcca
    15841 tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga tccgcctgat
    15901 gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg cgacccgcgg
    15961 tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac cgccggttat
    16021 tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca ccgaaatggt
    16081 ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc aggattacgc
    16141 agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta acgtgatttc
    16201 tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg ccttacgata
    16261 gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc aaattttata
    16321 gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg actggctgta ctgtgtattt
    16381 ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat caattattgc
    16441 cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac gacctcggac
    16501 gtgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc gcatttgtgg
    16561 tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg gcgacacacg
    16621 gcctccgctc agcggtgctt aaccatattc tggatacatt actggcgatc ccttcgctgc
    16681 tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct atgtttgctg
    16741 tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg catgacgaac
    16801 tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg aatattctct
    16861 ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc cgcgcactgt
    16921 cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca caactcccct
    16981 cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc gccccgtgga
    17041 ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac ctccttggcg
    17101 atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg atattcgtaa
    17161 cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc gggtaagtat
    17221 gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg gcaagagttt
    17281 gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg ctgaccgtat
    17341 gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca aactggttgg
    17401 ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt cagaacgtgt
    17461 gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt ggtggcagcg
    17521 ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga ttaaagatca
    17581 caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc agaaagtgat
    17641 gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac cgacaaactc
    17701 aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca accaaaacag
    17761 caataccact attttgctta tcagccatga cttacaaatg cttagccaat gggcggataa
    17821 aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg agttggtgac
    17881 gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact tcggcagcgc
    17941 gatgccgcat aaaagtcgcc tcaatactct acccggcgct atcccattgc tggaacagtt
    18001 accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat gcattgtgac
    18061 gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc tgaacatgga
    18121 gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa acgttccgct
    18181 accggaccgg ttggtttcgt cgtcagaccg tagaagcggt aaaacccttg agctttacgc
    18241 tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa tccacactgg
    18301 caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt gacgatcatc
    18361 cgctgcattt tggcgattat tccttccgta gtcagcgtat tcgcatgatt tttcaggatc
    18421 cttcgacctc gttgaatccc cgtcaacgta tctcacaaat tctggatttt ccactgcgcc
    18481 tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg atgcgtatgg
    18541 ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc ggacaaaaac
    18601 agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt gccgatgaag
    18661 ccctcgcttc actggatatg tcgatgcgtt cgcagttgat taatctgatg ctggagttac
    18721 aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg atgaagcaca
    18781 ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc agcaccgcgg
    18841 atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg cactttggcg
    18901 aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc tccttccaga
    18961 ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat ggataaaata
    19021 aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa gctctggcga
    19081 tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc ggtggggagc
    19141 tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga tggtgggcag
    19201 catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc gcagggctgg
    19261 accgtgctgc gtttctggaa taacgagatt gattgtaatg aggaggcggt gctggagatt
    19321 attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg ggccgaccga
    19381 gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc ggcaaggtct
    19441 gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt ttgttgctgg
    19501 ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt tagggtaagg
    19561 ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta tagctactca
    19621 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
    19681 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
    19741 cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct acggtatcgc
    19801 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
    19861 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt
    19921 tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
    19981 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
    20041 taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
    20101 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
    20161 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
    20221 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
    20281 cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag acttccgcaa
    20341 aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt
    20401 gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtagt
    20461 ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga aataatcgtt
    20521 ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
    20581 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
    20641 tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat
    20701 gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga agccgcaaag
    20761 gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc ccccttcctt
    20821 tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca gcttaccgtg
    20881 acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac ggttgctcag
    20941 gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc gctccccgga
    21001 cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata tgcccgccaa
    21061 ctggctagct cgcctgctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt
    21121 cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg ctttcaggcg
    21181 cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt acccgggctg
    21241 gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc
    21301 agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc agaactttgc
    21361 caactggccg aaacacactc tctccaccct atttcacttt cccttctcga aaagttgtta
    21421 aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt atgcgcgcgt
    21481 ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga atataaaaaa
    21541 atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga ccgtcattct
    21601 gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
    21661 gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat tattaatctg
    21721 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
    21781 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
    21841 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
    21901 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct ttggcttcct
    21961 ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
    22021 tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg cagagccaga
    22081 agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
    22141 tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc gcgcagcccg
    22201 tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc
    22261 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggcgacga
    22321 ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag aagcaccaga
    22381 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga cctcgctgga
    22441 ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt tcgctaaggc
    22501 gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
    22561 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
    22621 cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa
    22681 tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
    22741 taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt atgaccaggt
    22801 ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa ttgccgaaca
    22861 agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata accacgcact
    22921 agtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
    22981 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc
    23041 taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct ataacgtgca
    23101 tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct
    23161 gcatgtcgat gccgaagaag tactcacgct ggacggtttc tgcaaactgc gtcgtgaact
    23221 ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
    23281 aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg ccacctggac
    23341 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
    23401 aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg aacgtcgccg
    23461 tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct tcctgaaaga
    23521 taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc gtggcggcat
    23581 gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt tcttacacgc
    23641 tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta aaggtgaaac
    23701 ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca tggaaacccg
    23761 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct catggatggg
    23821 ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac tgactttcgt
    23881 ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa acaatgcgtt
    23941 tatccgccgt taatttcatg taagtcatgt tgttcctttt atttaaccgc ggactccgct
    24001 gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt gtacaacttt
    24061 ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgcttt gcgcttttct
    24121 accaccaaaa ccacagaacc tgatcgtaca ttgcagttat ctgccgaaca ggctgctcgc
    24181 atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt cgatgaaagc
    24241 gatctttctg agcatctggt aggacgaaaa atcaataaac gggaatgggc tggcaacgcg
    24301 tctgcatggc atgacacacc ggcggttgct cgtgatttat cacacgggct ttcctttgct
    24361 gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg ggggaatatc
    24421 caacgcttca atcggttatg tgaagattac acagggttga aagaacacga cgtcattggg
    24481 caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg cggcatccag gcgcaataac
    24541 cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc aacacgtaaa
    24601 ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg caaaaacgag
    24661 atttttttaa tctgttccgg taccgacatt accgaagagc gccgcactca ggagcgactg
    24721 cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc aatgcaggag
    24781 ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt gtatcttgat
    24841 ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga ccagttatta
    24901 cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt ggcgcgtcca
    24961 ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct ggaagcaatg
    25021 gcatcacgaa ttttaacccg cttacgcctc ccctttcgta ttggtttaat tgaagtttat
    25081 accagctgtt cagtaggtat ttcgctctct cccgaacatg gttcggacag cgcgactatt
    25141 attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg acaattttgc
    25201 gtttttactc cagaaatgaa tcagcgggta tttgaatatc tctggctgga taccaacttg
    25261 cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc acctagcgtg gcgaagtgcg
    25321 cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc caccgttgga
    25381 cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt gggtgattct
    25441 cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc gagtggcggt
    25501 aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga aacaggttct
    25561 ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga gttgtctgat
    25621 tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag gtgcgcaagt
    25681 gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc gctttccgat
    25741 cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac ctgtctcgca
    25801 gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg tgatcgccga
    25861 aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca atgagcggca
    25921 aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta
    25981 tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg tcattatata
    26041 tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta gcggcaattt
    26101 ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg aatttgatac
    26161 ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca
    26221 ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg ttgctgaatc
    26281 tacgccagac atcccgaaga caccgtggga gatttaacaa tcgtgccttc aggttttcct
    26341 atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt tataagaatt
    26401 acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca gggtcaggtt
    26461 agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg ccaggatctc
    26521 aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt ttcgcttgat
    26581 agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga actcgccgag
    26641 tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg cagcagcaat
    26701 gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat cacggtcagc
    26761 agctacatcg cgcatttgct gaaagacgcg ccttgtgaag tcattttgct cggtggcgtg
    26821 taccagaaaa aaagcgaaag tatggttggc cctttgacac gccagtgcat ccagcaggta
    26881 catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg attcaccggt
    26941 cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg aaaaagagtg cgaagcgata
    27001 gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg tcccgttgag
    27061 cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat gcatcttgag
    27121 cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc tacggatgat
    27181 ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta agactattta
    27241 cctgttatta acactctcaa gatataaaat tattatcagc gatataacag gaagtaatta
    27301 tcacgtgcgt gatataaccc tgcgcgcgag cagatttcac ggaataattt caccaggctt
    27361 attcttagct attatagtta tagagagctt acttccgtga atcataaatt caggagagag
    27421 tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta ctttggcaat
    27481 gtctctgagt gcctgttcta actggtctaa acgggaccgc aacaccgcaa tcggcgcggg
    27541 tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta cattaggtgg
    27601 tgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa taaatgtcga
    27661 ttatcgactc gcaattataa tacgctt
//
LOCUS       DADWTH010000048        27536 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.048, whole genome shotgun sequence.
ACCESSION   DADWTH010000048 DADWTH010000000
VERSION     DADWTH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.048"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JNY02_002187"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JNY02_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBB9433715.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="JNY02_002188"
     CDS             complement(1614..1937)
                     /locus_tag="JNY02_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBB9433716.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JNY02_002189"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JNY02_002189"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBB9433717.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JNY02_002190"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JNY02_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBB9433718.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDIYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="JNY02_002191"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="JNY02_002191"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBB9433719.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGVAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JNY02_002192"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JNY02_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBB9433720.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JNY02_002193"
     CDS             7537..8574
                     /locus_tag="JNY02_002193"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBB9433721.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JNY02_002194"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JNY02_002194"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBB9433722.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JNY02_002195"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JNY02_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBB9433723.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JNY02_002196"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JNY02_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBB9433724.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JNY02_002197"
     CDS             10745..11626
                     /locus_tag="JNY02_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9433725.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JNY02_002198"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JNY02_002198"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBB9433726.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JNY02_002199"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JNY02_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBB9433727.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JNY02_002200"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JNY02_002200"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBB9433728.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JNY02_002201"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JNY02_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBB9433729.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JNY02_002202"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JNY02_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBB9433730.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JNY02_002203"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JNY02_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBB9433731.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JNY02_002204"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JNY02_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBB9433732.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHACLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JNY02_002205"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JNY02_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBB9433733.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JNY02_002206"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JNY02_002206"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBB9433734.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JNY02_002207"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JNY02_002207"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB9433735.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JNY02_002208"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JNY02_002208"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBB9433736.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JNY02_002209"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JNY02_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBB9433737.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JNY02_002210"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JNY02_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBB9433738.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JNY02_002211"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JNY02_002211"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBB9433739.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JNY02_002212"
     CDS             24893..25273
                     /locus_tag="JNY02_002212"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBB9433740.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JNY02_002213"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JNY02_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBB9433741.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JNY02_002214"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JNY02_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBB9433742.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27536
                     /locus_tag="JNY02_002215"
     CDS             26442..>27536
                     /locus_tag="JNY02_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HBB9433743.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6792 a         6856 c         7219 g         6669 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa atatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgcca ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag gcagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaattg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgatac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcatgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagt atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc actggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga aaacatacag cccggacggg gctgta
//
LOCUS       DADWTH010000049        26934 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.049, whole genome shotgun sequence.
ACCESSION   DADWTH010000049 DADWTH010000000
VERSION     DADWTH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.049"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..438
                     /locus_tag="JNY02_002216"
     CDS             <1..438
                     /locus_tag="JNY02_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBB9433744.1"
                     /translation="LIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQ
                     VELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAW
                     FTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(500..1120)
                     /gene="rhtB"
                     /locus_tag="JNY02_002217"
     CDS             complement(500..1120)
                     /gene="rhtB"
                     /locus_tag="JNY02_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBB9433745.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1231..2253
                     /gene="pldB"
                     /locus_tag="JNY02_002218"
     CDS             1231..2253
                     /gene="pldB"
                     /locus_tag="JNY02_002218"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBB9433746.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWHQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2261..3061
                     /gene="yigL"
                     /locus_tag="JNY02_002219"
     CDS             2261..3061
                     /gene="yigL"
                     /locus_tag="JNY02_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBB9433747.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3137..4036
                     /gene="bioP"
                     /locus_tag="JNY02_002220"
     CDS             3137..4036
                     /gene="bioP"
                     /locus_tag="JNY02_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBB9433748.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3924..4877)
                     /gene="metR"
                     /locus_tag="JNY02_002221"
     CDS             complement(3924..4877)
                     /gene="metR"
                     /locus_tag="JNY02_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBB9433749.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5113..7374
                     /gene="metE"
                     /locus_tag="JNY02_002222"
     CDS             5113..7374
                     /gene="metE"
                     /locus_tag="JNY02_002222"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBB9433750.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7413..8228)
                     /locus_tag="JNY02_002223"
     CDS             complement(7413..8228)
                     /locus_tag="JNY02_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBB9433751.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8490..9251
                     /gene="udp"
                     /locus_tag="JNY02_002224"
     CDS             8490..9251
                     /gene="udp"
                     /locus_tag="JNY02_002224"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBB9433752.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9392..10819
                     /gene="rmuC"
                     /locus_tag="JNY02_002225"
     CDS             9392..10819
                     /gene="rmuC"
                     /locus_tag="JNY02_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBB9433753.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSR"
     gene            10914..11669
                     /gene="ubiE"
                     /locus_tag="JNY02_002226"
     CDS             10914..11669
                     /gene="ubiE"
                     /locus_tag="JNY02_002226"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBB9433754.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11683..12288
                     /gene="ubiJ"
                     /locus_tag="JNY02_002227"
     CDS             11683..12288
                     /gene="ubiJ"
                     /locus_tag="JNY02_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBB9433755.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12285..13925
                     /gene="ubiB"
                     /locus_tag="JNY02_002228"
     CDS             12285..13925
                     /gene="ubiB"
                     /locus_tag="JNY02_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBB9433756.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHRVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14004..14273
                     /gene="tatA"
                     /locus_tag="JNY02_002229"
     CDS             14004..14273
                     /gene="tatA"
                     /locus_tag="JNY02_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBB9433757.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            14277..14792
                     /gene="tatB"
                     /locus_tag="JNY02_002230"
     CDS             14277..14792
                     /gene="tatB"
                     /locus_tag="JNY02_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBB9433758.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14795..15571
                     /gene="tatC"
                     /locus_tag="JNY02_002231"
     CDS             14795..15571
                     /gene="tatC"
                     /locus_tag="JNY02_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBB9433759.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15613..16395
                     /gene="tatD"
                     /locus_tag="JNY02_002232"
     CDS             15613..16395
                     /gene="tatD"
                     /locus_tag="JNY02_002232"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBB9433760.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16392..16880)
                     /gene="rfaH"
                     /locus_tag="JNY02_002233"
     CDS             complement(16392..16880)
                     /gene="rfaH"
                     /locus_tag="JNY02_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBB9433761.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17047..18540
                     /gene="ubiD"
                     /locus_tag="JNY02_002234"
     CDS             17047..18540
                     /gene="ubiD"
                     /locus_tag="JNY02_002234"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBB9433762.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVVTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18586..19287
                     /gene="fre"
                     /locus_tag="JNY02_002235"
     CDS             18586..19287
                     /gene="fre"
                     /locus_tag="JNY02_002235"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBB9433763.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19380..20543)
                     /gene="fadA"
                     /locus_tag="JNY02_002236"
     CDS             complement(19380..20543)
                     /gene="fadA"
                     /locus_tag="JNY02_002236"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBB9433764.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20553..22742)
                     /gene="fadB"
                     /locus_tag="JNY02_002237"
     CDS             complement(20553..22742)
                     /gene="fadB"
                     /locus_tag="JNY02_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBB9433765.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGALRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22932..24263
                     /gene="pepQ"
                     /locus_tag="JNY02_002238"
     CDS             22932..24263
                     /gene="pepQ"
                     /locus_tag="JNY02_002238"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBB9433766.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24263..24877
                     /locus_tag="JNY02_002239"
     CDS             24263..24877
                     /locus_tag="JNY02_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBB9433767.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24916..26367
                     /gene="trkH"
                     /locus_tag="JNY02_002240"
     CDS             24916..26367
                     /gene="trkH"
                     /locus_tag="JNY02_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBB9433768.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26379..26924
                     /gene="hemG"
                     /locus_tag="JNY02_002241"
     CDS             26379..26924
                     /gene="hemG"
                     /locus_tag="JNY02_002241"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBB9433769.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6165 a         6858 c         7432 g         6479 t
ORIGIN      
        1 ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggtgggcgg tggcctgtat
       61 ctctgctgga tgggttacca gatgctacgc ggtgcactga aaaaagaggc tgtatctgcg
      121 cctgcaccac aggttgagct ggcgaaaagc gggcgcagtt tcctgaaagg cttactgacc
      181 aatctcgcca atccgaaagc gattatctat tttggttcgg tattctcatt gtttgtcggc
      241 gataacgttg gcactaccga gcgctggggc attttcgcgt tgattattgt cgaaacactg
      301 gcgtggttta ccgtcgttgc cagtctgttt gccctgccgc aaatgcgccg tggttatcaa
      361 cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt tggcattcat
      421 ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa ggcgtttacg
      481 ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct cccaccagca
      541 taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct ttaatccata
      601 gagcaatcct ttgagcaagg gtggcgtaac cgatcatcac aataatatcg accacaatag
      661 tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg atgaattgcg
      721 gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca aaaactgcgc
      781 gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaagatca attgcgccag
      841 cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc cactttaaca
      901 cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca accagcacaa
      961 tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg cgataaccgt
     1021 ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg cttaaaatga
     1081 tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc cggtgtgtct
     1141 atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgctt aaaaaaacga
     1201 ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga ctgggaaaca
     1261 agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg gcgtcagcgt
     1321 gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg ttttcgcgca
     1381 cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta cgtaaaatat
     1441 gcggaactgg cctacgacct gttccatttg gggtttgatg tcttaatcat cgaccatcgc
     1501 gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt taatcgcttt
     1561 aatgattatg ttgatgatct ggcggcattc tggcatcagg aggttcagcc cggtccgtgg
     1621 cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt atttctgcaa
     1681 cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg gatcgtgatt
     1741 cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca tccacgtttc
     1801 cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc tatcaacgta
     1861 ctgacccaca gcagacagcg atatcgacgt aacttacgct tctatgctga tgacccaacg
     1921 attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc tggcgaacag
     1981 gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttacaagc tgaagaggaa
     2041 cgcgtggtgg ataaccgcat gcacgaccgt ttttgtgaac tccgcaccgc cgcgggccat
     2101 cctgtcgaag gaggacggcc gttggtaata aaaggtgctt accatgagat cctttttgaa
     2161 aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg attttttcaa caggcataac
     2221 tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg ttgttgcgtc
     2281 tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg ccaaagaaac
     2341 tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc gtcaccacgt
     2401 tgatgtgggg caaattcgcg ataatctgga gattaagtct tacatgatta cctccaatgg
     2461 tgcgcgcgtt cacgatctgg atggcaatct gatttttgct cataacctgg atcgcgacat
     2521 cgccagcgat ctgtttggcg tagtcaacga caatccggac atcattacta acgtttatcg
     2581 cgacgacgaa tggtttatga atcgccatcg cccggaagag atgcgctttt ttaaagaagc
     2641 ggtgttcaaa tatgcgctgt atgagccggg attactggag ccggaaggcg tcagcaaagt
     2701 gtttttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga ttaacgctcg
     2761 ctggggcgat cgcgtcaacg tcagtttctc taccttaacc tgtctggaag tgatggcggg
     2821 cggcgtgtcg aaaggccatg cgctggaagc agtggcgaag aaactgggct acagcctgaa
     2881 ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga tggcggggaa
     2941 aggctgcatt atgggcagtg cgcaccagcg tctgaaagat ctgcatccgg agctggaagt
     3001 tattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct atttatcgta
     3061 atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat actcgttaat
     3121 caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg ccttctcctt
     3181 tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg tgctggtgcg
     3241 cgttggcttg gcggcactcg tttttctgcc gtttctgcgt acccgtagca atagcctgaa
     3301 aacggtcggc ctgtatatgc tggtgggcgc gatgcagctc ggcgtgatgt atatgctgag
     3361 tttccgcgct tatctctacc tgacggtctc cgaattgttg ctgttcaccg tactgacgcc
     3421 gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct ggggctatgc
     3481 ttttagcgcc ttgctggcgg tgattggtgc cgggattatt cgctatgatc aggtcaccga
     3541 ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg ccatcggcat
     3601 ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg cctttgcgtg
     3661 gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca
     3721 gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg tggtggcttc
     3781 cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg gaacgctggg
     3841 cattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta tctggcacca
     3901 acagccgcac tggccaacgt ttattacagg cgcgctggtg atcctggcct cactgtgggt
     3961 gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca ggcgtgattg
     4021 cgcgctgagc gaataaacgc ttccgtaact ggctgacgct gctcgccatc gcgcacggcg
     4081 gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag accctggcgc
     4141 tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc ggcaaccatt
     4201 tgaatcaata ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc tgccggctga
     4261 agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaattaa tagcgtctcg
     4321 ctggcgagat cttccggtgt aatacgcgtt tttgccgcca gtggatggtc aggtgctaac
     4381 accaggcgta cttcataatc gaacatcggc gaataatgca ggccactgcg cggcaggata
     4441 tcggaggtca tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg gtcaaacgtc
     4501 acgcccgatt taaaatccat ctctacctgc ggccagttct tatggaaatt ttctaaagcg
     4561 ggtgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg cgtctgctgc
     4621 ggttcattgc aggcttgcag tgcctggctg atttgcggca gcacctggtt tgccagttgc
     4681 aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac aaatagccgg
     4741 aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg cgtctgatgc
     4801 aacgttgccg cagcggctgc gagcgagccg cagttccgca acgcttgtag cgttttcagg
     4861 tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg cttgaggaat
     4921 atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaaatc cttcgtcttt
     4981 taaatttatg gtgcgttggc tgcgtttctc caccccggtc acttacttca gtaagctccc
     5041 ggcgatgaat aaacttgccg ccttccctaa attcaaaatc cataggattt acatataatt
     5101 agaggaagaa aaatgacaat attgaatcac accctcggtt tccctcgcgt tggcctgcgt
     5161 cgcgagctga aaaaagtgca agaaagttat tgggcgggga actccacgcg tgaagaactg
     5221 ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca agcgggtatc
     5281 gacctgctgc cggtgggcga ttttgcctgg tacgatcatg tactgaccac cagtttgctg
     5341 ctgggtaatg ttccgccacg tcatcagaac aaagacggtt cggtagatat cgacaccctg
     5401 ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc
     5461 aaatggttta acaccaacta tcactacatg gtgccggagt tcgttaaagg ccaacagttc
     5521 aaactgacct ggacgcagct gctggaggaa gtggacgagg cgctggcgct gagccacaac
     5581 gttaagcctg tgctgctggg gccggttact tacctgtggc tggggaaagt gaaaggtgaa
     5641 cagtttgacc gccttagcct gctgaacgac attctgccgg tttatcagca agtgctggca
     5701 gaactggcga aacgcggcat cgagtgggta cagattgatg aacccgcgct ggtactggaa
     5761 ctaccacagg cgtggctgga cgcatacaaa cccgcttacg acgcgctcca gggacaggtg
     5821 aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa atctcgacac gattactgcg
     5881 ctgcctgttc aggggctgca tgtggacctt gtacatggta aagatgacgt tgttgaactg
     5941 cacaagcgcc tgccttctga ctggctgctg tctgcgggtc tgatcaatgg tcgtaacgtc
     6001 tggcgcgccg atcttactga gaaatatgcg caaattaagg acattgtcgg caaacgtgat
     6061 ttgtgggtgg catcttcctg ctcgttgctg cacagcccca tcgacctgag cgtggaaacg
     6121 cgtcttgatg cagaagtgaa aagctggttt gccttcgccc tacaaaaatg ccatgaactg
     6181 gcactgctgc gcgatgcgct gaacagtggt gacacggcag ctctggcaga gtggagcgcc
     6241 ccaattcagg cgcgtcgtca ctctactcgt gtacataatc cggcggtaga aaagcgtctg
     6301 gcggcgatca ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg tgctgaagcc
     6361 cagcgtgcgc gttttaaact gccagcgtgg ccgaccacca cgattggttc cttcccgcaa
     6421 accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga cgccaacaac
     6481 taccgcacgg gcattgcgga acatatcaag caggccattg ttgagcagga acgtttggga
     6541 ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca tggtggaata ctttggcgag
     6601 cacctcgacg gatttgtctt tacgcaaaac ggttgggtac agagctacgg ttcccgctgc
     6661 gtgaagccac cgattgtcat tggtgacatt agccgcccgg caccgattac cgtggagtgg
     6721 gcgaagtatg cgcaatcgct gaccgacaaa ccggtgaaag ggatgctgac ggggccggtg
     6781 accatactct gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacag
     6841 attgcgctgg cgctgcgtga tgaagtggcc gatctggaag ccgctggaat tggcatcatc
     6901 cagattgacg aaccggcgct gcgcgaaggt ttaccgctgc gtcgtagcga ctgggatgcg
     6961 tatctccagt ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa agatgacaca
     7021 caaatccaca ctcacatgtg ttattgcgag ttcaacgaca tcatggattc gattgcggcg
     7081 ctggacgcag acgtcatcac catcgaaacc tcgcgttccg acatggagtt gctggagtcg
     7141 tttgaagagt ttgattatcc aaatgaaatc ggtcctggcg tctatgacat tcactcgcca
     7201 aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga aagcggcaaa acgcattccg
     7261 gcagagcgcc tgtgggtcaa cccggactgt ggcctgaaaa cgcgcggctg gccagaaacc
     7321 cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt tgcgtcgggg gtaaatccaa
     7381 accgggtggt aataccaccc ggtcttttct cattacagcg acttcttccc accatactgc
     7441 ttaaaccatt ccagcatacg ctgccagcca tctttcgcag attcggcatg atagctcggg
     7501 cgataatcag cgttgaatgc atgcccggca tccgggtaca cgataatctc tgctttcgca
     7561 ttagcagccc gcagcgcctg gcgcatcgtt tcaacgctct cctgcggaat gctgttatcc
     7621 tgaccaccat ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc aacaggttgt
     7681 ttcggtgaat tcagcgactt gtcgcccgtc agtttgccgt accacgccac tgcggctttt
     7741 agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca gaatccggtg
     7801 atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcgac atgatcgaga
     7861 tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa cgtggggata
     7921 tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat agccagatac
     7981 ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc
     8041 tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggtctggc atggtaagca
     8101 ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc gtcgtccggg
     8161 gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt
     8221 gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac tatagtcagc
     8281 atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc agaatgaaga
     8341 gtgatttatg tgatatgaat cacttttggt gggtaaattt atgcaacgca tttgcgtcat
     8401 ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt tgcttcttct
     8461 gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt catctcggcc
     8521 tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg
     8581 tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac cgcgaattca
     8641 ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg
     8701 gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc accttcctgc
     8761 gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc ctggttacca
     8821 cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg
     8881 tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatctatt ggcgcgacaa
     8941 ctcacgttgg cgtgacagtt tcttctgata ccttctaccc aggtcaggaa cgttacgata
     9001 cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg caggcgatgg
     9061 gcgtaatgaa ctatgaaatg gaatctgcaa ctctgctgac catgtgtgca agtcagggcc
     9121 tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag atcccgaatg
     9181 ctgagacgat gaaacaaact gaaagccatg cggtgaaaat cgtggtggaa gcggcgcgtc
     9241 gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg
     9301 cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca attctatttt
     9361 gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg tttacgcagt
     9421 tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac atgcgcagca
     9481 aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca
     9541 acaaattacc caaagcgagc actggcgtgc agagtgtgaa ttgctcaata acgaagtgcg
     9601 cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa ccacgcggat
     9661 ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca gcgagcagcg
     9721 cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca atcgccgggt
     9781 tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg aacaactgga
     9841 cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac gccataccct
     9901 gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg aagcgatcaa
     9961 cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg aggtagtatt
    10021 gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag
    10081 catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc tgccgcaggg
    10141 aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct attttaacgc
    10201 cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg tgcgtaacca
    10261 tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa ctctggatta
    10321 cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc gccagccgga
    10381 gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta cgctgctggt
    10441 ggcgttgcgt actattgcca acctgtggcg ttatgagcat caaagccgta acgcccagca
    10501 aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg atgacatgtc
    10561 cgcgattggt caaagtctcg acaaagcgca ggataactac cgccaggcaa tgaaaaaact
    10621 ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt taggagtaga
    10681 aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccagg atgaagagta
    10741 tcgacttcgg tcggttccgg ggcagccgaa tgatgaagct tatcaacgcg atgatgaata
    10801 taatcagcag tcgcgctagc ccattagggg tagttaagcc gggtagaaat ctagggcatc
    10861 gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt gagatggtgg
    10921 ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg
    10981 atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg aatgatttga
    11041 tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc
    11101 gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg aaattctccc
    11161 gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa
    11221 tgggacgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat gttcaggcga
    11281 acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt tcgtttggtc
    11341 tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg
    11401 gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg agcaaagcct
    11461 atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg aacgacgccg
    11521 acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat accctgaaag
    11581 ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg gcaggggttg
    11641 tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt taaaccttta
    11701 gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc cgcgctgaaa
    11761 acagcccgct cgcgtctgct gggtaaagta ttgcgcgtgg aggtaaaagg cttttcgacg
    11821 tcattgattc tggtgttcag cgaacgccag gttgatgtac tgggcgaatg ggcaggcgat
    11881 gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga tcgccagcag
    11941 cttaccgcac tgattcgcag tggtgagctg gaagtgcagg gcgatattca ggtggtgcaa
    12001 aacttcgttg cgctggcaga tctggcagag ttcgaccctg cggaactgct ggccccttat
    12061 accggtgata tcgccgctga aggaatcagc aaagccatgc gcggaggcgc aaagttcctg
    12121 catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga agagtggcgt
    12181 atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc cgtcgagcgt
    12241 gctgttgatg ccctgaccaa acggctggaa aaactggagg ctaaatgacg cctggtgaag
    12301 tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat gaactgatcc
    12361 ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc tggatgccaa
    12421 atcggcataa agacaaactt ttaggtgagc gactacgact ggccctgcaa gaactggggc
    12481 cggtttggat caagttcggg caaatgttat caacccgccg cgatcttttt ccaccgcata
    12541 ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc aagctggcga
    12601 agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt gacgattttg
    12661 aaatcaagcc gctggcttct gcttctatcg cccaggttca taccgcgcga ttgaaatcga
    12721 atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtc attaaagcgg
    12781 atctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc
    12841 gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat gaactgaatt
    12901 tgctgcggga atctgccaac gccattcagc ttcggcgcaa ttttgaagac agcccgatgc
    12961 tctacatccc ggaagtttac cctgactatt gtagtgaagg gatgatggtg atggagcgca
    13021 tttacggcat tccggtgtct gatgttgcgg cgctggagaa aaacggcact aacatgaaat
    13081 tgctggcgga acgcggcgtg caggtgttct tcactcaggt ctttcgcgac agctttttcc
    13141 atgccgatat gcaccctggc aacatcttcg taagctatga acacccggaa aacccgaaat
    13201 atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa cgctatctgg
    13261 cagaaaactt tatcgccttc tttaatcgcg actatcgcaa agtggcagag ctacacgtcg
    13321 attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgcc attcgtacgg
    13381 tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat gtactgttaa
    13441 atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg gtgttactcc
    13501 agaaaaccct gctctatgtc gaaggggtag gacgccagct ttatccgcag ctcgatttat
    13561 ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt attcctgccc
    13621 tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg
    13681 aactggttta cgacagtttg cggcagggca agtatttaca gcaccgtgtt gataagattg
    13741 cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt ctcggaattg
    13801 gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgag tgggggctga
    13861 tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac
    13921 gctgattttt tcatcgctca aggcgggccg tgtaacgtat aatgcggctt tgtttaatca
    13981 tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta ttgattattg
    14041 ctgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt tccgatcttg
    14101 gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa
    14161 ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg gatacgaatc
    14221 aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg taatccgtgt
    14281 ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc gttctggggc
    14341 cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac
    14401 tggcgacaac ggtgcagaac gaactgaccc aggagttaaa actccaggag tttcaggaca
    14461 gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac gcccgaactg aaagcgtcga
    14521 tggatgaact acgccaggcc gcggagtcga tgaagcgttc ctacgttgca aacgatcctg
    14581 aaaaggcgag cgatgaagcg cacaccatcc ataacccggt ggtgaaagat aatgaagctg
    14641 cgcatgaggg cgtaacgcct gccgctgcac aaacgcaggc cagttcgccg gaacagaagc
    14701 cagaaaccac gccagagccg gtggtaaaac ctgctgcgga cgcggaaccg aaaaccgctg
    14761 caccttcccc ttcgtcgagt gataaaccgt aaacatgtct gtagaagata ctcaaccgct
    14821 tatcacgcat ctgattgagc tgcgtaagcg tctgctgaac tgcattatcg cggtgatcgt
    14881 gatattcctg tgtctggtct atttcgccaa tgacatctat cacctggtat ccgcgccatt
    14941 gatcaagcag ttgccgcaag gttcaacgat gatcgccacc gacgtggcct cgccgttctt
    15001 tacgccgatc aagctgacct ttatggtgtc gctgattctg tcagcgccgg tgattctcta
    15061 tcaggtgtgg gcatttatcg ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc
    15121 gctgctggtt tccagctctc tgctgtttta tatcggcatg gcattcgcct actttgtggt
    15181 ctttccgctg gcatttggct tccttgccaa taccgcgccg gaaggggtgc aggtatccac
    15241 cgacatcgcc agctatttaa gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt
    15301 tgaagtgccg gtggcaattg tgctgctgtg ctggatgggg attacctcgc cagaagactt
    15361 acgcaaaaaa cgcccgtatg tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc
    15421 gccggatgtc ttctcgcaaa cgctattggc gatcccgatg tactgtctgt ttgaaatcgg
    15481 tgtcttcttc tcacgctttt acgttggtaa agggcgaaat cgggaagagg aaaacgacgc
    15541 tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg cccgtcaggg cggttgtcat
    15601 atggagtaca ggatgtttga tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac
    15661 cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta atgggctact catcaccggc
    15721 actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg
    15781 tcaacggcgg gcgtacatcc tcacgacagc agccagtggc aagctgcgac tgaagaagcg
    15841 attattgagc tggctgcgca gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt
    15901 aaccgcaact tttcgacgcc ggaagagcag gaacgcgctt ttgttgctca gctacgcatt
    15961 gccgcagaat taaacatgcc ggtatttatg cactgtcgcg atgcccacga gcggtttatg
    16021 acattgctgg agccgtggct ggataaactg cctggtgcgg ttcttcattg ctttaccggc
    16081 acacgcgaag agatgcaggc gtgcgtggcg cgtggaattt atatcggcat taccggttgg
    16141 gtttgcgatg aacgacgcgg gctggagctg cgggaattgt tgccgttgat tccggcggag
    16201 aaattgctga tcgaaacaga tgcgccgtat ctgctcccgc gcgatctcac gccaaagcca
    16261 tcatcccggc gcaacgagcc agcccatctg ccccatattt tgcaacgtat tgcgcactgg
    16321 cgtggagaag atgccgcatg gctggctgcc accacggatg ccaatgtcaa aacactgttt
    16381 gggattgcgt tttagagttt gcggaactcg gtgttcttca cactgtgctt aatctcttta
    16441 ttaataagat taagcaatag catggagcga gcctcaccat cgggttcggt gaaaatggcc
    16501 tgaaagcctt cgaacgcgcc ttcggtaata atcaccttat cacccggata agggggggcc
    16561 ggatcgacaa tgtctttcgg tttatatacc gatagctgat gaatcaccgc cgatgggact
    16621 atcgctggcg acgcgccaaa gcgcacaaag tggctgacgc cacgcgtagc gctgatggtc
    16681 gtggtatgaa tcacttctgg atcaaattcc acaaacaggt agttagggaa taacggttca
    16741 ctgactgcgg tacgtttacc ccgtacgatt ttttccaggg tgatcatcgg agcgaggcaa
    16801 ttaaccgcct gtctctcaag gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac
    16861 agtaaatacc aggattgcat aatgactctt atccgtttaa tcggggcgca aggatagcaa
    16921 aagctttacg ctaagttaat tatattcccc ggtttgcgtt ataccgtcag agttcacgct
    16981 aatttaacaa atttacagca ttgcaaagat gaacgccgta taatgggcgc agattaagag
    17041 gctacaatgg acgccatgaa atataacgat ttacgcgact tcctgacgct gcttgaacag
    17101 cagggtgagc taaaacgtat cacgctcccg gtggatccgc atctggaaat cactgaaatt
    17161 gctgaccgca ctctgcgtgc tggtgggcct gcgctgttgt tcgaaaaccc taaaggctac
    17221 tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc gcgtggcgat gggcatgggg
    17281 caggaagatg tttcggcgct gcgtgaagtt ggtaaattat tggcgtttct gaaagagccg
    17341 gagccgccaa aaggtttccg cgacctgttt gataaactgc cgcagtttaa gcaggtgtta
    17401 aacatgccga caaagcgact gcgtggtgcg ccctgccaac aaaaaatcgt ctctggcgat
    17461 gacgtcgatc tcaatcgcat tcccattatg acctgctggc cggaagatgc cgcgccgctg
    17521 attacctggg ggctgaccgt aacgcgcggc ccgcataaag agcggcagaa tctgggcatt
    17581 tatcgccagc agctgattgg taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc
    17641 ggcgcgctgg attatcagga gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct
    17701 gtggcgctgg gtgccgatcc tgccacgatt ctcggtgcag tcacccccgt tccggatacg
    17761 ctttcagagt atgcgtttgc cggattgcta cgcggcacca aaaccgaagt agtaaagtgt
    17821 atttccaatg acctcgaagt gccagccagt gccgaaatcg tcctggaagg gtatatcgat
    17881 cctggtgaga tggcgccgga agggccgtat ggtgaccaca caggttacta taatgaagtc
    17941 gatagtttcc cggtggttac cgtgacgcat attacccagc gtgaagatgc gatttaccat
    18001 tccacctata ccgggcgtcc gccagatgag cccgcggtac tgggcgtggc gttgaacgaa
    18061 gtgtttgtac cgattctgca aaaacagttc ccggaaattg tcgattttta cctgccgccg
    18121 gaaggctgtt cttatcgtct ggcggtagtg acgatcaaaa aacagtacgc cggacacgcg
    18181 aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt ttatgtacac taaatttgtg
    18241 atcgtttgcg atgatgacgt caacgcacgc gactggaacg atgtgatttg ggcgattacc
    18301 acccgtatgg acccggcgcg ggatactgtt ctggtagaaa atacgcctat tgattatctg
    18361 gattttgcct cgcctgtctc tgggctgggt tcaaaaatgg ggctggatgc cacgaataaa
    18421 tggccggggg aaacccagcg tgaatgggga cgtcccatca aaaaagatcc agatgttgtc
    18481 acgtatattg acgccatctg ggatgaactg gctattttta acaacggtaa aagcgcctga
    18541 tgcgcgtttg ttttgcccta tttatcgatc cgacagagaa agcgcatgac aaccttaagc
    18601 tgtaaagtga cctcggtaga agctatcacg gataccgtat atcgtgtccg catcgtgcca
    18661 gacgcggcct tttcttttcg tgctggtcag tatttgatgg tagtgatgga tgagcgcgac
    18721 aaacgtccgt tctcaatggc ctcgacgccg gatgaaaaag ggtttatcga gctgcatatt
    18781 ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg accgcatcct caaagatcat
    18841 caaatcgtgg tcgacattcc ccacggagaa gcgtggctgc gcgatgatga agagcgtccg
    18901 atgattttga ttgcgggcgg caccgggttc tcttatgccc gctcgatttt gctgacagcg
    18961 ttggcgcgta acccaaaccg tgatatcacc atttactggg gcgggcgtga agagcagcat
    19021 ctgtatgatc tctgcgagct tgaggcgctt tcgttaaagc accctggtct gcaagtggtg
    19081 ccggtggttg aacaaccgga agcaggctgg cgtgggcgta ctggtaccgt gttaacggcg
    19141 gtattgcagg atcatggtac gctggcagag catgatatct atattgccgg acgttttgag
    19201 atggcgaaaa ttgcccgtga cctgttttgc agtgagcgta atgcgcggga agatcgcctg
    19261 tttggcgatg cgtttgcatt tatctgagat ataaaaaaac ccgcccctga caggcgggaa
    19321 gaacggcaac taaaacttta ttcagtggca tttagatcta tgtcgcatcc ggcaagtggt
    19381 taaacccgct caaacaccgt cgcaataccc tgacccagac cgatacacat cgtcgccaga
    19441 ccaaactgaa cgtctttgcg ttccatcaga ttcagcagcg tggtgctgat acgcgcaccg
    19501 gaacaaccca gcggatgacc cagcgcgatc gcaccaccat tgaggttgat cttctcgtca
    19561 atctgctcca ttagcccgag atctttaatg cacggcagga tctgcgcggc aaaggcttcg
    19621 ttcatctcaa acacgccgat atcgctggca gaaagccccg cttttttcag cgccagcttc
    19681 gaggccggaa ccgggccgta acccataatc gatgggtcac aaccaacgac cgccatcgaa
    19741 cgcacacgag cgcgcggctt aagacctaat tcatgggcgc ggctttcact catcaccagc
    19801 atggcagctg cgccatcgga aagtgcagaa gatgtgcccg ccgttaccgt accgtttact
    19861 ggatcaaacg ccggacgcag cgtggcgagg gcttccacgg tggtttccgg gcgaatcact
    19921 tcgtcgtaat taaactgctt caggacgccg tcggcatcgt gaccaccagt tgggatgatt
    19981 tcatttttaa atgcggccga ctgcgtggcg gcccaggcgc gggcgtgtga ccgcgcggca
    20041 aaggcatcct gcatttcacg gctgataccg tgtatacgcg ccagcatttc tgccgttaag
    20101 cccatcatgc ccgccgcttt ggcgacattg cggctcaggc cggggtgaaa atcgacgccg
    20161 tgactcatcg gcacatggcc catatgctcc acgccgccaa ccagacatgc ctgcgcatcg
    20221 ccagtcatga tcattcgtgc tgcgtcatgc agtgcctgca tggatgaacc acacaagcga
    20281 ttgacggtaa ccgccgggac agagtgcggc acttctgcca gcagcgccgc gttacgggcg
    20341 atgttaaaac cctgctccag cgtctgttgc acacaacccc agtaaatatc gtcgagggcc
    20401 gccgcttcca gcgccgggtt acgcgccagc aggctacgca ttaaatgagc ggagagatct
    20461 tccgcacgca cgttacgaaa agcaccgccc ttcgaacggc ccatcggggt gcgaattgca
    20521 tcgacaatga caacctgttc cattgtgatt ccttaagccg ttttcaggtc gccaaccgga
    20581 cgggctggct caaccggagg atagtacggt tcgttatgac gcgctttatt acgcagacct
    20641 tccggcactt catacagcgg gccgaggtgc tgatattgct gtgccatatc gaggtatttt
    20701 gcgctaccga gggtgtccag ccagcggagc gcgccgccgt ggaacggagg gaagcccagg
    20761 ccgtagacca gcgccatatc cgcttccgcc ggagtggcga taatgccctc ttccagacag
    20821 cgcaccactt cgttgaccat cgggatcatc atgcgggcga taatctcttc ttcgctgaaa
    20881 tcgcgcttcg gttggctcac ttctgccagc aggtcttcaa cggcggcgtc ttcttctttc
    20941 ttcggcttac ctttgctgtc ttctttataa cgccagaaac cgaggccgtt cttctgacca
    21001 aagcggttgg catcaaacag cgcgtcgatg gcatcgcggt aatctttctg catccgctgc
    21061 gggaagcctg ctgccatgac agcctgagcg tgatgcgcgg tatcaatgcc cacaacgtcc
    21121 agcagatatg ccgggcccat cggccagcca aactgttttt ccatcacttt gtcgatcttg
    21181 cggaaatccg cgccgtcgcg cagcagctgg ctgaaaccgg cgaaatacgg gaacagcacg
    21241 cggttaacaa agaagccggg gcagtcgtta accacaatcg gcgtcttgcc catcttgctc
    21301 gcccaggcga caactttcgc gatggtttcg tcggagcttt tctcgccgcg aataatttct
    21361 accaacggca ttcggtggac cgggttaaag aagtgcatcc cgcagaagtt ttccgggcgt
    21421 tccagcgcgt tggccagttc gctgatagga atggttgaag tgttagacgc cagcacggta
    21481 tcctggcgta ctttttgttc ggtttctgcc agtacggctt ttttcacttt cgggttttca
    21541 acaaccgctt ctaccacaat atccacgcgg tcaaatccgg cgtagtcgag cgttgggtgg
    21601 atcgtggaga tcacgccagc cagtttcaga ccatcgatct tgccgcgctc aagctgcttg
    21661 ttcagcagtt tcgcggcttc ggtcatgccg agggttaacg acttgtcgtt gatatctttc
    21721 atgacaaccg gcacgccttt ccacgcggac tggtaagcga taccgccacc catgatgcct
    21781 gcgcccagca cagcagcctg tttcggggtt tcaacgtctt tggtgagttt cttcgctttg
    21841 ccttttacat attgatcgtt aaggaaaatg ccgaccagtg cgcgagcttc gttggtatgc
    21901 gccagcggga caaaactttt gttttccaga tttaaggctt cttcacgacc aaaacgggcc
    21961 gcagcttcaa tggtttttac tgcggtgatg ggggccggat aatgtttccc cgccgtttgc
    22021 gcgaccattc ctttagcgat ggtgaagctc atggtggctt caatcttgct cagttttagc
    22081 ggttccagct tcggctgacg tttcgctttc cagtcgaggt cgccgttaat ggcctggcgt
    22141 aaaatcgcca tcgccccttc aatcagtttt tctgctttga ctacgccatc caccagaccg
    22201 attttcagcg cctgatccgc gccgacatct ttaccggcgg cagtgatttc cagtgcactg
    22261 tcagcgccca gcatacgtgg catacgtaca gaaccgccaa agcccggcat gatgcccagt
    22321 ttggtttccg gcagaccgat gcgcagatcc ggcgtcgcca gacgataatc ggtcgccagc
    22381 acgcattcgc agccaccacc cagcgcatag ccgttgacgg cagcaatggt cggcaccggc
    22441 agatcttcca ggcgattaaa cacgctattg gcaaagtgca gccactgact taactgttct
    22501 tcaggaacga ggaacaggga caaaaattcg gtgatatcag caccgacgat aaaggctgct
    22561 ttgttcgaac gcagcagcag cccttttaga tctgattgct gttccagcac gccgatggcc
    22621 tcgccgaggc tggcgacggt cgcagtgtcg agtttattaa ctgaacctgg ggcatcaaat
    22681 accagttcgg caatgccatc ttccagccag tcaaggtaca gggtgtcgcc tttgtaaagc
    22741 atgtcagtct cctgaatccg caaggtgatc tggtcgtacc agatgcatcg gagtgtgtgt
    22801 tttgtgttaa aaatatgcaa ataaaagatt aaagaaatgc cgatctgatc acgctcggca
    22861 gaaatcacgc tctggatgaa cgatgtgcta agatgcggag acttaaggtc aaaaaaacag
    22921 aagggtaaaa aatggaatca ctggcctcgc tctataaaaa tcatatagct accttacaag
    22981 aacggactcg cgatgcgctg gcgcgcttca agctggatgc attacttatt cactccggcg
    23041 agctgttcaa cgtttttctc gacgatcatc cctatccgtt taaagtgaac ccgcaattca
    23101 aagcgtgggt gccggtaact caggtgccaa actgctggtt gctggtggat ggcgtgaaca
    23161 agccgaaact gtggttctat ctgccggttg attactggca caacgtcgaa ccgctgccga
    23221 cctccttctg gactgaagat gtggaagtga tcacgctgcc gaaagccgat ggcattggta
    23281 gcctgctgcc tgctgcgcgc ggcaatatcg gttatatcgg tccggtgccg gaacgtgcgc
    23341 tgcaactggg tattgaggcc agcaacatca acccgaaagg ggtgatcgac tacctgcatt
    23401 attaccgctc cttcaaaacc gagtacgaac tggcctgtat gcgtgaagcg cagaaaatgg
    23461 cggtcaatgg tcatcgtgcg gcagaagaag cgttccgttc tggcatgagc gagttcgata
    23521 tcaatattgc ctatctgacc gcgaccggtc atcgtgatac cgacgtacct tacagcaaca
    23581 ttgtggcgct taacgaacac gctgcggtgt tgcattacac caaactggac caccaggcac
    23641 cggaagagat gcgcagcttc ctgctggatg ccggggccga atataacggc tatgcggctg
    23701 acctgacccg tacctggtcg gcaaaaagcg acaacgacta cgcacagctg gtgaaagacg
    23761 taaatgatga acaactgggg ctgatcgcca ccatgaaagc aggcgtcagc tatgtggatt
    23821 accacatcca gttccatcag cgcatcgcca aactgctgcg taaacatcaa atcatcaccg
    23881 atatgagtga agaagcgatg gtcgaaaacg atctcaccgg accgtttatg ccgcacggta
    23941 ttggccatcc gctgggcctg caggtgcatg acgtcgccgg ttttatgcag gatgatagcg
    24001 gtacacacct cgcggcaccg gcaaaatatc cgtacctgcg ctgcacccgt attctccagc
    24061 cgggcatggt gttaaccatc gaaccgggta tctacttcat tgaatcgctt ctggcaccgt
    24121 ggcgtgaagg gcagttcagc aagcacttca actggcagaa aattgaagca ctgaaaccgt
    24181 tcggcggcat tcgtatcgaa gacaacgtgg tgatccacga aaacaacgtg gaaaacatga
    24241 cccgggatct gaaactggcg tgatggaaag ctggttaatt cctgcggcac cggtcacggt
    24301 cgttgaagag atcaaaaaga gccgcttcat aacgatgttg gcgcataccg atggcgttga
    24361 ggcggcgaaa gcgtttgttg aatcggtgcg ggcagaacac cccgatgccc gccaccattg
    24421 cgtggcgtgg gtggcgggtg cgccggatga ttctcaacag ctgggtttct ctgacgacgg
    24481 ggagccggcg ggaacggcag gtaaaccgat gctcgcccag ctaatgggca gcggcgtcgg
    24541 ggaaattacc gctgtggtag tgcgctacta cggcggcata ttgcttggca ccggtgggtt
    24601 agtgaaagcg tatggcggcg gcgtgaatca ggcgctgcgc cagctaacga cccaacgcaa
    24661 gacgccatta accgaatata ctttgcaatg tgaatatagt cagttaaccg gcattgaagc
    24721 gttgctgggg cagtgtgacg gcaaaattat caacagtgat tatcaggcat tcgttctgct
    24781 gcgggtggcg cttccggcgg cgaaagtggc tgaattttcc gcaaagctgg cggattttag
    24841 ccgtggttca ttgcaattgt tagcgattga agaataatcc ccacttcgtt ttgcagaact
    24901 aaggaagcgg cagagatgca ttttcgcgcc attacccgaa tcgttggact actggtcatc
    24961 ttattttcag ggaccatgat tatccctggg ctggtagcac tcatctaccg ggatggagcg
    25021 ggccgcgctt ttacccagac cttttttgtc gccctcgcca ttggctctat gctgtggtgg
    25081 ccgaaccgca aagagaaggg cgaactgaaa tcccgtgagg ggtttctgat agtggtgctg
    25141 ttttggaccg tgctgggtag tgtcggtgcg ctccctttta tcttctcgga aagcccgaac
    25201 ctcacgatta ccgatgcgtt ttttgaatct ttctctggcc tgaccaccac cggggccact
    25261 acgctggtgg ggctggattc gctccctcat gccatcctct tttatcgcca gatgctgcaa
    25321 tggtttggcg ggatggggat catcgtgtta gcggttgcga tactgcctat cctcggcgtg
    25381 ggtgggatgc agctctatcg cgcagaaatg cccggcccgc tgaaagataa caaaatgcgc
    25441 ccgcgaattg cggaaacggc gaaaactctg tggttgattt atgtcttgct gaccgtcgcc
    25501 tgtgcgctgg cgttgtggtt tgccggaatg gatgcctttg atgccatcgg ccatagcttt
    25561 gcgactatcg ctattggcgg cttctcgaca catgatgcca gtatcggtta tttcgacagt
    25621 ccggctatta acactatcat tgctatcttc ctgctgatct ccggctgtaa ctacggtctg
    25681 cacttttcac tgttaagtgg gcgtagtctg aaggtttatt ggcgcgatcc ggaatttcgc
    25741 atgtttatcg gcgtacagtt tacgctggtg gttatttgta cactcgtact gtggtttcat
    25801 aatgtctaca gttcggcgct gatgacaatt aaccaggcgt ttttccaggt ggtatcgatg
    25861 gcgacaaccg ccgggtttac gactgacagc attgcccgct ggccgctctt tttgccggta
    25921 ctgcttttat gttcagcttt tatcggcggt tgcgccgggt caacgggcgg tggcctgaaa
    25981 gtgatccgca tcctgctgct gtttaagcag gggaaccgtg agctgaaacg actggtgcat
    26041 ccgaacgccg tctatagcat taagctgggg aatcgcgcac tgccggaacg tatcctcgaa
    26101 gccgtttggg gatttttctc cgcctatgca ttggtgttta ttgtcagtat gctggcgatt
    26161 atcgccacgg gcgtggatga tttttctgcc tttgcctcag ttgttgcgac attgaataac
    26221 ctggggccag ggcttggcgt ggttgctgat aactttacca gtatgaaccc ggtggctaaa
    26281 tggatcctga ttgccaacat gctgtttggt cgtctcgagg tctttacatt gctggtgctc
    26341 tttaccccga ctttctggcg tgaatgatgg agtaatacgt gaaaacatta attcttttct
    26401 caacaaggga cggacaaacg cgcgagattg cctcctacct ggcttcggaa ctgaaagaac
    26461 tggggatcca gacggatgtc gccaatgtgc accgcattga agaaccacaa tgggaaaact
    26521 atgaccgtgt ggtcattggt gcttctattc gctatggtca ctaccactct gcgttccagg
    26581 aatttgtcaa aaaacatgcg acgcgactga attcgatgcc gagcgccttt tattccgtaa
    26641 acctggtggc gcgcaaaccg gagaagcgta ctccacagac caacagctac gcgcggaagt
    26701 ttctgatgaa ctcgcaatgg cgtcccgatc gctgcgcggt cattgccggg gcgctgcgtt
    26761 acccacgtta tcgctggtac gaccgtttta tgctcaagct gattatgaag atgtcaggcg
    26821 gtgaaacgga tacgcgcaaa gaagttgtct ataccgattg ggagcaggtg gcgaatttcg
    26881 cccgagaaat cgcccattta accgacaaac cgacgctgaa ataagcataa agaa
//
LOCUS       DADWTH010000050        26411 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.050, whole genome shotgun sequence.
ACCESSION   DADWTH010000050 DADWTH010000000
VERSION     DADWTH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.050"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            118..1089
                     /locus_tag="JNY02_002242"
     CDS             118..1089
                     /locus_tag="JNY02_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB9433770.1"
                     /translation="MRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVR
                     QGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVR
                     RASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKI
                     AVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLN
                     AIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKM
                     GNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1112..2623
                     /locus_tag="JNY02_002243"
     CDS             1112..2623
                     /locus_tag="JNY02_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBB9433771.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2648..3646
                     /locus_tag="JNY02_002244"
     CDS             2648..3646
                     /locus_tag="JNY02_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9433772.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTGLSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3679..4773
                     /locus_tag="JNY02_002245"
     CDS             3679..4773
                     /locus_tag="JNY02_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBB9433773.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4785..5657
                     /locus_tag="JNY02_002246"
     CDS             4785..5657
                     /locus_tag="JNY02_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBB9433774.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5705..6127)
                     /locus_tag="JNY02_002247"
     CDS             complement(5705..6127)
                     /locus_tag="JNY02_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBB9433775.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6224..7430)
                     /locus_tag="JNY02_002248"
                     /pseudo
     CDS             complement(6224..7430)
                     /locus_tag="JNY02_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="JNY02_002249"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="JNY02_002249"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBB9433776.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPT
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8249..8569)
                     /locus_tag="JNY02_002250"
     CDS             complement(8249..8569)
                     /locus_tag="JNY02_002250"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBB9433777.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8569..9087)
                     /locus_tag="JNY02_002251"
     CDS             complement(8569..9087)
                     /locus_tag="JNY02_002251"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBB9433778.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9084..10445)
                     /locus_tag="JNY02_002252"
     CDS             complement(9084..10445)
                     /locus_tag="JNY02_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBB9433779.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10581..11471
                     /gene="hcaR"
                     /locus_tag="JNY02_002253"
     CDS             10581..11471
                     /gene="hcaR"
                     /locus_tag="JNY02_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBB9433780.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11631..12770
                     /locus_tag="JNY02_002254"
     CDS             11631..12770
                     /locus_tag="JNY02_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBB9433781.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="JNY02_002255"
     CDS             complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="JNY02_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBB9433782.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKHFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAVLAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(14233..15087)
                     /locus_tag="JNY02_002256"
     CDS             complement(14233..15087)
                     /locus_tag="JNY02_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB9433783.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTSPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="JNY02_002257"
     CDS             complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="JNY02_002257"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBB9433784.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16154..16894
                     /gene="trmJ"
                     /locus_tag="JNY02_002258"
     CDS             16154..16894
                     /gene="trmJ"
                     /locus_tag="JNY02_002258"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBB9433785.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17164..17652
                     /gene="iscR"
                     /locus_tag="JNY02_002259"
     CDS             17164..17652
                     /gene="iscR"
                     /locus_tag="JNY02_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBB9433786.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17764..18978
                     /gene="iscS"
                     /locus_tag="JNY02_002260"
     CDS             17764..18978
                     /gene="iscS"
                     /locus_tag="JNY02_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB9433787.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19006..19392
                     /gene="iscU"
                     /locus_tag="JNY02_002261"
     CDS             19006..19392
                     /gene="iscU"
                     /locus_tag="JNY02_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBB9433788.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19409..19732
                     /gene="iscA"
                     /locus_tag="JNY02_002262"
     CDS             19409..19732
                     /gene="iscA"
                     /locus_tag="JNY02_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBB9433789.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19828..20343
                     /gene="hscB"
                     /locus_tag="JNY02_002263"
     CDS             19828..20343
                     /gene="hscB"
                     /locus_tag="JNY02_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBB9433790.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20360..22210
                     /gene="hscA"
                     /locus_tag="JNY02_002264"
     CDS             20360..22210
                     /gene="hscA"
                     /locus_tag="JNY02_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBB9433791.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22212..22547
                     /gene="fdx"
                     /locus_tag="JNY02_002265"
     CDS             22212..22547
                     /gene="fdx"
                     /locus_tag="JNY02_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBB9433792.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22559..22759
                     /gene="iscX"
                     /locus_tag="JNY02_002266"
     CDS             22559..22759
                     /gene="iscX"
                     /locus_tag="JNY02_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBB9433793.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22818..24101
                     /gene="pepB"
                     /locus_tag="JNY02_002267"
     CDS             22818..24101
                     /gene="pepB"
                     /locus_tag="JNY02_002267"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBB9433794.1"
                     /translation="MTEAMKITISTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24243..25019
                     /gene="sseB"
                     /locus_tag="JNY02_002268"
     CDS             24243..25019
                     /gene="sseB"
                     /locus_tag="JNY02_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBB9433795.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="JNY02_002269"
                     /pseudo
     CDS             complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="JNY02_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6256 a         6584 c         7313 g         6258 t
ORIGIN      
        1 cgttcactaa accctattaa ctgtaatatt tgagcggcac gcgctgccgc tcatcacaaa
       61 ataattagaa taatccgttg ttaataaaaa ccctacagga ggcactatgc ctaaaaaatg
      121 agaacaaccc gtaatctatt attaatggct acgctattag gtagcgcact atttgccagg
      181 gctgcggata aagaaatgac cattggtgca atataccttg atacccaggg atattacgct
      241 ggagtgcgtc agggcgttca ggatgcggca aaagattctt cagtacaggt acagttaatt
      301 gaaactaacg cccaggggga tatttcgaaa gaaagtacct ttgttgatac cctcgtggcg
      361 cgtaatgtcg atgccattat tttatcggca gtgtctgaaa atggcagtag ccgtaccgtt
      421 cgtcgcgcca gtgaagcggg tattccggtg atttgctaca acacctgtat taatcaaaag
      481 ggtgtcgata aatatgtctc ggcgtatctg gtcggcgatc cactggaatt tggtaaaaaa
      541 ctgggtaacg ctgccgccga ttattttatt gccaataaaa ttgaccagcc gaaaattgcc
      601 gtcatcaatt gcgaagcctt tgaagtttgt gtgcagcgac gtaaaggatt tgaagaagta
      661 ttaaaatccc gcgttcccgg cgcgcaaatt gtcgctaacc aggaagggac tgttttagat
      721 aaagcgattt ccgttggtga aaaactgatt atctccacgc cggatctcaa cgccattatg
      781 ggggagtcgg gcggtgcgac actcggcgcg gtaaaagcgg tacgtaatca aaatcaggcc
      841 ggaaaaattg ctgttttcgg ttcggatatg acaaccgaaa ttgctcagga gctggaaaac
      901 aatcaggtgc tgaaagcggt ggtggatatt tccggtaaga aaatgggcaa tgctgttttc
      961 gcgcaaacat tgaaggttat caataaacaa gccgacggtg aaaaagtgat tcaggtgcca
     1021 atcgatctct ataccaaaac ggaagatggt aaacagtggc tggcaacgca cgttgatggt
     1081 ctgccctaat cgctgattcg cagaggtccc tatgttcacg gcaacagagg cagtcccggt
     1141 agcaaaagtg gtggcaggaa ataagcgtta ccccggcgtc gttgcgctgg ataacgttaa
     1201 cttcacgctc aataaaggcg aagttcgtgc gctgttaggt aaaaacggcg cgggcaaatc
     1261 gactctcatt cgaatgctta ccggcagcga acgcccggat agcggtgata tctggattgg
     1321 cgagacgcga ctggaaggtg acgaagctac gttgactcgc cgtgccgctg aactgggggt
     1381 tcgtgcggtt tatcaggaat taagtctggt ggaagggctg acggtggcag aaaacctctg
     1441 ccttggtcaa tggcctcgcc gcaacggcat gattgattac ctgcaaatgg cgcaggatgc
     1501 ccaacgttgc ttacaggcgc tgggcgttga cgttagtcct gaacaacttg tttcaacgct
     1561 aagcccggcg caaaagcaac tggtggaaat tgcacgggtg atgaagggcg agccgcgcgt
     1621 ggtcattctt gatgaaccca ccagctcgct tgccagtgca gaagttgaac tggtgatcag
     1681 tgcggtgaaa aagatgtcgg cactgggcgt ggcggtgatt tatgtcagcc accggatgga
     1741 agaaatccgc cgcattgcct cctgtgccac cgttatgcgc gatggtcagg tggcgggcga
     1801 tgtgatgctc gaaaacacct ccacgcatca tattgtgtcg ctaatgctag ggcgtgatca
     1861 cgttgatatt gcgccggttg cccctcagga aattgtggat caggccgtac tggaagtccg
     1921 tgcgttacgc cataagccca agctggagga tatcagcttt acgctacgtc gtggcgaagt
     1981 gcttggcatt gctggcctgc tgggggcagg gcgcagtgaa ttgctgaagg cgattgttgg
     2041 gctggagacg tatgaacagg gcgaaattgt tatcaacggc gagaaaatca tgcgccccga
     2101 ttacggcgac atgctgaaac gcggcattgg ctatacgcca gaaaaccgca aagaagcggg
     2161 gatcattccc tggctgggcg ttgacgaaaa tacagtgctg accaatcggc aaaaaatcag
     2221 caccaacggt gtgctgcaat ggtccaccat ccgccgcctg accgaagagg tgatgcagcg
     2281 gatgacggtc aaggctgcca gtagcgaaac acccatcggc acgctttctg gtggcaatca
     2341 gcaaaaagtg gtgatcggtc gttgggtcta tgccgccagc cagattttgt tgctcgacga
     2401 gccaacgcgc ggcgtcgata tcgaagccaa acagcagatt taccgtattg tccgtgagct
     2461 ggctgccgaa ggaaaaagcg tggtgtttat ctccagtgaa gtggaggaat tgccgctggt
     2521 gtgtgaccgc atcctgttat tacagcacgg cacgttctcg caggagtttc actcgccggt
     2581 caatgtggat gagctgatgt ccgccattct gtctgtgcac tgatgaattt taccgaggaa
     2641 cctgaagatg tctgcttcgt cattaccatt gccgcagggc aagagcgtct cgctcaaaca
     2701 atttgtcagt cgccatatta atgagatcgg tttgctggtg gtgattgcca ttctttatct
     2761 ggtcttctcc ctgaacgcgc ctggctttat ctcattgaat aaccagatga acgtgctgcg
     2821 cgatgccgcc accattggga ttgccgcctg ggcgatgacg ctgattatta tctccggtga
     2881 aattgatgtc agcgttgggc cgatggtggc ttttgtctcg gtgtgcctgg catttttgct
     2941 gcaatttgaa gttccgttgg cgattgcttg tctgctggtg ttgctgttag gcgcgctgat
     3001 gggaacgctc gccggggtgc tgcgcggcgt gtttaacgta ccaagtttcg ttgccacact
     3061 ggggctgtgg agcgctctgc gcggaatggg gctgtttatg accaacgcac agccagtgcc
     3121 gattgacgaa aacgaggtgc tggactggct gggcggacaa tttctcggtg tgccagtatc
     3181 cgcgctgatc atgatggtgt tgtttgcgct gtttgtgttc attagccgaa aaaccgcctt
     3241 cgggcgctcg gtttttgccg ttggcggtaa tgccacggcg gcgcagttgt gcggtattaa
     3301 cgttcgtcga gtgcgcattc ttatctttac cctttcggga ttattagcgg cggtgaccgg
     3361 cattttgttg gcggcacgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt
     3421 tgacgtcatc gccgcggtcg tggtcggcgg tacgggactt tccggcggtc gcggttccct
     3481 gttcggtaca ttgcttggtg tgctggtgat tacgctaatc ggtaacggtc tggtgctgct
     3541 cggtattaac tcctttttcc agcaggtggt acgcggcgtc atcatcgtgg tggcggtgct
     3601 ggcgaatatc ttgctgaccc agcgaagcag taaagcgaaa cgctaaacct taacgcccct
     3661 ggcgcgggtg cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg
     3721 atgctggcag cttatttacc aggaaattca accgtcgatc tgcgggaagt tgcggtgccg
     3781 acgccgggga ttaaccaggt actgatcaaa atgaaatcct ccgggatttg cggaagcgat
     3841 gtccactata tctatcatca acaccgtgcc acggcggcgg cacccgataa accgttatac
     3901 cagggattta tcaacggtca tgaaccgtgc gggcagattg tggcgatggg gcaaggctgc
     3961 cgccatttta aagagggcga ccgcgtgctg gtgtatcaca tttctggctg tggtttttgc
     4021 ccgaactgcc gccgcggctt tcctatctct tgtactggca aaggaaaagc ggcttacggc
     4081 tggcagcgtg acggcggtca tgccgaatat ctgctggcgg aagaaaaaga tctgatcctc
     4141 ctgccggatg cgctgagcta cgaagatggt gcgtttatca gttgcggcgt tggcacggcc
     4201 tatgaaggga ttttgcgcgg cgaagtttcc ggcagcgata acgtgctggt ggtcggtctg
     4261 ggggcggtcg gcatgatggc gatgatgctg gcgaaaggtc gcggtgcaaa acggatcatc
     4321 ggcgttgata tgctgccgga acgtctggcg atggcaaaac agttaggggg gatggatcac
     4381 ggctatttag caaccaccga aggtctgccg cagataattg ctgaacttac ccacggtggc
     4441 gcggatgttg cgctcgattg ttccggtaat gccgcaggtc gcttgctggc actgcaatcc
     4501 actgctgact gggggcgggt ggtttacatt ggtgaaaccg gaaaagtgga attcgaggtc
     4561 agcgctgatc tgatgcacca tcaacggcgg attatcggct cctgggtgac cagtctgttc
     4621 catatggaaa aatgcgccca cgatttgact gactggaagc tgtggccgcg taacgccatt
     4681 acccatcgct tctcgctaga acaggcaggt gatgcctatg tgctgatggc gagcggcaaa
     4741 tgcgggaaag ttgtgattaa cttcccggat taaggagtta gcggatgacc atctatacct
     4801 tatcccacgg gtcgctaaag ctggacgttt ctgaccaggg cggcgtaatc gaaggcttct
     4861 ggcgcgatac gacgccgctc ttacgtcctg gtaaaaaaag cggtgtggca accgatgcct
     4921 catgttttcc gctggtgcca tttgcaaacc gggtgagcgg caaccgtttt gtctggcagg
     4981 ggcgtgagta tcaactgcaa ccgaatgttg agtgggatgc gcactatcta cacggcgatg
     5041 gctggctggg cgagtggcaa tgtgtctcgc acagcgatga tagtttgtgt ctggtgtatg
     5101 aacatcgcag cggtgtctat cactatcggg taagtcaggc gtttcattta acggcggata
     5161 cgctgacggt gacgctctct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg
     5221 gctggcatcc ttattttccg ttgtcaccac aaacgcggat tcaggcgcag gcgagcggtt
     5281 actggctgga acgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac
     5341 tggattttaa ccagctcgcg ccgttgccgc gtcagtgggt gaacaatggt tttgccggat
     5401 ggaatggtca ggcccgcatc gagcagccgc aggaaggtta tgccatcatc atggaaacga
     5461 cgccacctgc accgtgttat ttcatctttg tttccgaccc tgcgtttgat aaaggatatg
     5521 cgtttgattt cttctgtctg gaaccgatga gccatgcgcc ggatgatcat catcgcccgg
     5581 aagggggtga tctcattgcg ctagcaccag gggaatcaac aacctcagag atgtcgttgc
     5641 gggtagagtg gctgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc
     5701 tttcctaacg ccgatcgaga gaaattgccc ccggtccggt aattgccaat agcaagaacg
     5761 cgccagcgat actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg
     5821 tcatatccca gtaatggtga ccaatcaccg ccgtacccag cgtgtagaaa ataaacagca
     5881 ccgccagcgg acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa
     5941 ctaccgcaat aatcgctgcc agcatcggca ttggcgcgcc caacgaggcc atatattgga
     6001 ccgtaccgtc aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg
     6061 cgatacgggc aattaataac aaaacggggc gggcagctcc aaaatcaaaa taacgtagtg
     6121 tgttcataac caatcccttt gcaagataat gtgatttaaa cgtaatacac actcttgcac
     6181 ctcgccacgc cccaggcatt gagatattcg ctaattatcc tggttacagt gatttaagcg
     6241 cgatgttctc atctatcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc
     6301 gaattgggcg aatctcacgc ccctgattca gcgtgaccgc accgataagc acgccgtttt
     6361 gcagattaaa ccaaatcgcc ttctgagttt ccgggttgcc acgacaaagc cagtcatcgc
     6421 cacgcatatc gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg
     6481 gcggcagtag cggtagccct aacattgcgg cagcggcaat ttgcgcctgg ttattggtgt
     6541 tttcccagct ttcgcagcgg tgtagtgcac cattatcaag acgagtgatt gccacatcgc
     6601 caccggcaaa gatcgcggga tcgcaggtgc ggcaagcctc atcaatgaca atgccattgg
     6661 cagtatcaag gttggcctcg cgagccagtt gctcgttggc gctgatacta ataccgtaaa
     6721 tcaccacatc agcctgaagc gtttccccac tttgcagcgt cagttctact ttttcaccat
     6781 cgaccacatg ttcaatggca ttattgagca gaatgcgcac accagcctgc tgatggcgct
     6841 gtaaaagata gcgttgcacg ggcggtggtg cattacggcc catgacggtt gccgccagtt
     6901 caatcactgt caccttacat ctgcgctgcg ctgcgctgcg tggcgctggc agccagttcc
     6961 agaccaatag ttccggcacc gacaatcacg actgaccgtt cgggctgcag aacttctcgc
     7021 agtctggcgg catcaccggc atggtgtagg gtaaagcagc gttctcccag tgcatcaagc
     7081 aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa gctgatccca gtgccagctt
     7141 tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca gcgttttgat ggttacaccg
     7201 gaatgcagat gaacattgtt ttcctgccac cagttagcgg gtaacacctg ctgtaactgc
     7261 ggggaatctt ccagcaacat ggatttcgag agtggaggtc gttcataagg aagatgtcgc
     7321 tcatcggaaa acagatgcag ctcaccggtg aacccttgct ggcgtagcga ggccgcagcc
     7381 attgccgccg cttgcccgcc accgacaata atgatcgttt tttctttcat cgtgcttcct
     7441 taaagatcca gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg
     7501 ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat cacatacggc
     7561 cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta aaatggcggc aattttctcc
     7621 ggcgtcagag actgcattat cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg
     7681 tcgctggcca taccacacgg gccgacgcca ttcacccgca ctttcggtgc cagttcataa
     7741 gccagttggc gaataagtcc ggttgcggca tgtttactgg tggtgtacag cgggccaccg
     7801 ccgccaggat accaggcggc atttgacagt gtgaaaatca tgctgccttc actggcgatt
     7861 aacgccggag cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac gttaaacagc
     7921 tcgtggaagc cggtttcgag cgtctctgtg ggagtattaa ccagtgaggc attgtgatcc
     7981 cagatgcctg cattgccgat aaaacaatcc agcttgccgg aacgagtcag gatctgatcg
     8041 accgcgcgtt gataatcggc ataacaggtc acgttacctt ccaccgccag aatatgttcg
     8101 ccaaatcgct gacgcagact ggcgactttt gccgccgaca gttccagcgc ggcaacctgc
     8161 gcgccttctt cgataaatcg ctcgaccagc gccagcccta atcccgatcc gccgccggta
     8221 ataaaaatgg actcgttatg cagatcgctc atggctgcgc ctccggtaag tcgatgaaaa
     8281 tgtcaccacc ttcaacgtgt actggataag tggtgagcgg atcggtggcg ggcaggcata
     8341 acgctttccc cgttttcagg caaaaactgg cggcgtgtag cgggcactcc accgtggcgt
     8401 catcttccag atacccttct gacatcgacg cattaccatg gctgcaacga tcgttaatgg
     8461 cataaaactc gccgcccacg ttgaacaagg cgatgacggg cgaggtatct atccggagag
     8521 cctcaccctc cggcacatcc gcaacgggac acgcataaat tcgattcatc agaacagtac
     8581 actcaggtta tgggaagtga tcaccgcttg atccaggacg atatcccgtt ccagcaagcg
     8641 ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc
     8701 atcgcgctct ttttgtgccc gataaagtag ataattcacc ctcacggcaa atacgtttgg
     8761 gatgtcggtt tcgcttacct ggcagttgct gattaagtga cgagtgcgtg acggcggctc
     8821 ttctgcccag gccatgcccg tttccagacg ggcgattcgg cgctccagct ggtctttggt
     8881 gtcattgaaa atccaggtcg tcggcggctg gacgcctttg cgacggtcgc gtgtttgcgc
     8941 gttaaccgtg gtgcgcatgg tgtaacgaat ctcttcgtcg agctgtgcca accagtcacg
     9001 aaatttccag tcatccagca gactggcttc gtgaaagaga aactggctaa tgcggtgatg
     9061 taactccagt gaaacttgcg cactcatttc atcacctcct gctggtaagc ggcggtttta
     9121 tcgagcactt cctgccagct ttcgctactc agaagatcgg cccagcgttg gtacattcca
     9181 cgagcggcag tttctgaaaa gatatagtta gtaatgccag gaatgccgtc gtcgcggcgc
     9241 ttttcctgac caagccccat ttccagacac agtttgctgt tgcgggcgcg gtggccttta
     9301 agcaatttct ggatttcaca ccagttctcc gagtcatcct gctcgagaaa accagcagga
     9361 ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa cttcatcgga ggcggctttg
     9421 tcagtaatac agaacgccca cacttcaact tgatcagggc cgcgcggatg ccagacgcgg
     9481 agtgtggcag tgccgtttag ccatgaaagc gtggggaata tattgttatg acccgccagg
     9541 cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg cataggtttc gcggtaatag
     9601 cttgaaactg cgccatcgac ccagacatta gcatccggtt tttcagtaaa gaagaaaccg
     9661 ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg cggtttccca caccgggcgg
     9721 gcggtttgtc catcaccgag gcgcttatcg ctgccatcat ctttcgcccc taatacctga
     9781 acggcagaag catggctgaa cagagcatga tactggtcac tggcgaactg ctctgccggg
     9841 aatttccagt tacagttgat cacccacttt tgtacgccgc cgacaatttc ggtgccgcct
     9901 tcgcgacgat ccagcatgcc atccagatac caggcaatgt cacccaggta atcatgcagg
     9961 cccggtgcgc tggtatccca gttgccaaaa atcagtcctt tataactctc cacacaagga
    10021 acttcgttta gcccccagtg ggatttacac aacccttgtg ggtaggcgcg aggttccagc
    10081 ggtacatcga tcaactcgcc gttaatgcca taagaccagc cgtgatacgg gcaggtaaag
    10141 gcgcgagtgt tgccacaatc tgcataactc acacgcatgg cccggtggcg gcattggtta
    10201 agaaacgcct tgatgctgcc gtctttctgg cgcaccacga caactgcatc ttctcccatg
    10261 taggtgttaa agaaatcacc gggttttggg atctggcttt cgtgggcgag aaatagccag
    10321 caacgaccga aaatacgctc aagctccagt tggtaaatgt ccgggtcggt ataaatacgc
    10381 ggagtgaccc gaccgttttg ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg
    10441 gtcatatttt ttaatcctga aagataagat gtattgacct tctatttttg tgccataagg
    10501 atgttgctgg ttggttgcta atatgtgaaa tagttaattt ctaccagctg agacgttttt
    10561 tcgataacaa ggggaaggtg atggaactac ggcatttacg ctatttcgtc gcagtggcgc
    10621 aggcactgaa ctttacccgt gcggcggaaa aactgcatac ctcacagcct tcgttaagta
    10681 gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt actggtgagg gataagcgca
    10741 aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga cgcgctggca atcctcgaac
    10801 aagcggaaaa tgccaaatta cgggcgcgga aaattgttca ggaagacaga caattaacca
    10861 ttggctttgt gccatcggcg gaagtgaatt tattgccaaa agtattaccg atgtttcgtc
    10921 tcagacagcc agacaccttg attgagctgg tgagtttaat caccacgcaa caggaggaaa
    10981 aaattcgccg tggtgaactc gacgtcggct taatgcgcca tcctgtttat agcccggaga
    11041 ttgattatct ggagcttttt gacgaaccat tagtggtggt gttaccggtt gatcaccctt
    11101 tagcacatga aaaagagatc accgccgcgc aactggatgg cgtgaatttc gtcagtaccg
    11161 atccggcgta ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca
    11221 gccagccaaa tatcgtccag gtggcaacga atattctggt gaccatgaat ctggtgggca
    11281 tggggctggg cgtcactttg atacccggtt atatgaataa ttttaatacc gggcaggttg
    11341 tttttcgtcc cattgccgga aatgtgcctt ccattgcttt actgatggcg tggaagaaag
    11401 gggagatgaa gccggcatta cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg
    11461 taacggcata attacgcatt atttttgcaa catgcgccgc ggatcgcagt tttttagtgc
    11521 ttgtgaactg gcgttacaga catcgggaag caggctcgaa tattcttatt tttcaacttg
    11581 caccaacatt cgattaaccc cagactaacg cctcctgacg ggagggactc atggttttgc
    11641 aatccacgcg ctggttggcg ctcggctatt tcacatactt ttttagttac ggcatttttt
    11701 tacctttctg gagcgtctgg cttaaaggga ttggtttaac gccagaaacc atcggcctgt
    11761 tattgggggc aggtctggtt gcccgttttc tcgggagttt gctcatcgcg ccccgcgtca
    11821 gcgatccttc ccgcctgatt tccgccttgc gcgtgctggc actgctgcca cttctctttg
    11881 ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg attggcttta
    11941 acctcttttt ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc
    12001 agttcccgct tgattacggc aaagtgcgac tgtggggctc ggtggcgttt gtcattggct
    12061 cggcgctgac gggcaaactg gtcactatgt ttgattatcg ggtgatcctc gtgctgttga
    12121 cgttgggcgt ggcatccatg ctgctcggct ttctcatccg tccgacgatt cagccacaag
    12181 gggcaagccg ccagcaggag agcaccggtt ggtctgcgtg gttggcgctg gttcgccaga
    12241 actggcgctt tctggcctgc gtttgtttat tgcagggggc acatgcggcc tattacggtt
    12301 ttagcgccat ttactggcag gcagctggct actcggcctc ggcggtgggg tatttgtggt
    12361 cgctgggcgt ggtggcggaa gtcattatct ttgcgctgag taataaactt ttccgccgtt
    12421 gtagtgctcg cgatatgctg ttgatctcgg cgatttgcgg cgtagtgcgc tggggcatta
    12481 tgggagcaac tacggcgttg ccgtggttga tagtggtgca aattctgcat tgcggcacct
    12541 tcacggtctg ccacctggcc gccatgcgtt atattgctgc tcgccaggtt agcgaagtca
    12601 tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg cagtatcgct atcatgaccg
    12661 ttttcgccgg tttcctgtat caatatctgg gccacggtgt gttctgggta atggcgctgg
    12721 tggcgcttcc ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt
    12781 ttgcggatct gctcctgttg atgtgttgtc agcgtcaggt ccgtatagat cagcggtggt
    12841 gaaaagagcg gtaacggcat ggtataaggc gcaataatca gtgccgcgcc acgcggagcg
    12901 cctgtctgca aaaatgcctt taccgacata tgcttaatgt tgagcggcag taacgttagt
    12961 tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat cattcccggt cagtagaata
    13021 atctgttttt catgcagatc gttttcctgc attagccagg caccgaaaat caccgcgacc
    13081 agaccacttt cctcatcaga aagatggacg ccgtattcac tttcaaatcc ggccagcacc
    13141 gcgcgggtgg tgcgtaccag gcgtggatac agtctggcga actcttccgg cagggtatta
    13201 tcaataccga tggcaaacaa actgcgattt aacgcctggg cgaggtggat ataaagctga
    13261 tcacataacc cctgttcatc ataaaaacga acatttccca gctcacgaaa atggtttacc
    13321 agacgtttga tagactgacg caattgtctg tcccgctgat gcgcatcccg cagtggatcg
    13381 ggaacccgca acatggaaaa aagtaatgcc ataaacagtg gctcatcagg cggtacatgc
    13441 tggagcgccc gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag
    13501 gattccgccc agcgacgttg gagcggatta aactgaggcg taattccagc gtggtgctgc
    13561 agcaaacaat attgcagata cagacacagg aaatgaatat cgcgcgattc aaagtgtttt
    13621 tgcagccgtc gggagcagag attcactaac gcttgtagat tggtatcgtc gtaaaagtta
    13681 cgcgcaattc cgcgccgctt cagttcactc ttcagggcgg gggtgaattg gctggtaata
    13741 aatgacggac acagacggaa accacgtcgt agccagtgaa ataaacacaa acgttggtta
    13801 agcactgtac cttcaacccg gtagctaccg tcataaccag tcgtcagtgt gagttgatga
    13861 tagcgcagga tctcccgtcc tgtttcgctg atatcaagac tggcaatatc atcatccaca
    13921 ccattaagct cgctgaaggt tgccatggtg gcggtcaacc ccggctgaaa gagcgtcagc
    13981 aagatctggc agcggcgctg gggagccgaa aggacagatg gtggagcaag cgtaggcatc
    14041 atctgtagat ctcccataag cataattgat aagaatagct aaagtttgct cagggaaatg
    14101 gcgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt tctcatttta
    14161 aactgttctg accagtggat tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct
    14221 agcgatcatg ggttagttct gcggattcaa cgcgctaagg ataaagtcca ccatctgttc
    14281 gcgataaaca ttgccgtgac gatcggaaaa agcatcaatg tgttcgccat ccgggattag
    14341 gatcaaccgt tttggctctt ttgccaggct atacaacttt tcgctgtgct gccatgggat
    14401 aacgtgatcg gctttaccgt gaatgagtaa aagcgggatc gggctgacgc tggcgatgta
    14461 attttcgccg ctgtaactct catcaagtaa gtagccactg ccggggatca tctggttggc
    14521 gatggttgca taagaggcaa atgtggagtc gaggatcacc gcacgtatgc cttcacgatc
    14581 accctgacca ataacagcaa gaatattcgc cccgccaata ctctgcccga acagcaccag
    14641 ccgttgtggg tttacatcac tgcgatggcg caccacattg atggcgcttt gcgtatcgtc
    14701 cagcaatccg gcctgggacg gagtgccttt tgatttacca aacccgcgat aatcaaacat
    14761 aaaaacgttg aaattacgct caggtaacca actgaccagc ggccagtggg cggacatatt
    14821 tccggcattg ccgtgagcat gaatgatggt tgcgatggcg ttgtcagcag ggctcgtcga
    14881 agaaggaata aaccagcctt gcagacgagt accgtcctta gccgtaaatt cgacggattc
    14941 cgccgaccag gggtcgggac cgtaaatttt gtcgtcagga taatagaaga agttgatggc
    15001 aacgcgcagc actaaataaa cgcagaaggc aacaacaaag agaataaaca gaattttggg
    15061 aacgcgtttg ttcactggca gtgccataac ctttccttgc gacttcttta tcatccatta
    15121 atcggggaaa gatggtggga gagtgacgaa agaaagtaaa gcggtgagga agtatttcgg
    15181 aatagcgcct gaaaggcgag ggcgggtgag tgatatcacc cgcctgagtc attaacgctt
    15241 cagagcgtcg cttaactcgt cacgcatgtt cgccagtatg gctttcacaa cacgcgggtt
    15301 accagcaacg atgttaccgg tcagcatata gttatgacca ccggtgaagt cgctgacgat
    15361 gccgcccgct tcacgaacca gcagctcgcc tgccgcgaaa tcccacggac gcagaccgat
    15421 ttcaaagaaa ccgtcaacac gacccgcagc gacgtaagcc agatccagcg ccgcagaacc
    15481 ggtacgacgg aagtctgcac attcgttgaa cagcttgccg acgatgttga tgtaggtagt
    15541 ggcgtactgt tttgctttga acgggaagcc ggtcgccaga atggtaccgt cgagatcgcg
    15601 agcggtgcta ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt
    15661 gaacagttcg ttacgcatag gatcgtatac cacagcaact tcggtgcggc ctttgatacg
    15721 aacggcgata gataccgcga agtgcggcag acgtttgata aagttggtag taccatccag
    15781 tggatcgata acccattgaa catcctgatc agtaccttca agttcaccgc tttcttcggt
    15841 gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg cttcggcagc
    15901 tttatctacg ttggtcacga aatcgttact gcctttctgg ctcgcttcta cagcgtccgg
    15961 ggtttcatag tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt
    16021 cagcatcgga tgcatcggtc tctctcactg gatgttaaag aacgggaaaa cggcgcagag
    16081 tatagcagcg agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa taatctttag
    16141 atgaagaaag acaatgctgc aaaatattcg aattgtgctg gtggagacgt cacacaccgg
    16201 caatatgggt tctgttgccc gtgccatgaa aacaatggga ttaaccaatc tgtggctggt
    16261 taatccactg gtgaaacccg actcccaggc gattgccctg gctgcagggg ccagcgatgt
    16321 gattggtaat gctcacatcg tcgatacgct cgacgaagcg ttagccggtt gtagcctggt
    16381 ggtgggcacc agcgcacgct cccgcacgct gccgtggccg atgctcgacc cgcgcgaatg
    16441 cggtctgaaa agcgtcgctg aagcggcaaa taccccggtg gcgctggtgt ttggtcgtga
    16501 gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat tatcatgtcg cgattgcggc
    16561 aaacccggaa tacagttcgc tgaacctggc aatggcggtt caggttattg cctatgaagt
    16621 gcgtatggcc tggctggcaa ctcaggaaaa tggcgagcag gtcgagcatg aagagacgcc
    16681 gtatccgctg gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa cgctgctggc
    16741 aaccggtttt atccgtgaaa accatccggg gcaggtgatg aataaattgc gccgtctgtt
    16801 tacccgtgcg cgcccggaaa gccaggaatt gaatatcctg cgcgggattc tggcttctat
    16861 tgagcagcag aataaaggta acaaggccga ataacagtgg ttgcctgatg cgacgcttaa
    16921 cgcgtcttat caggcctaca atgtacagaa tcgtaggtcg gataaggtgt tcacaccgca
    16981 tccgacaaca ggtacaaacg ccacgataaa aaaatggcac tgaaggttaa atacccgact
    17041 aaatcagtca agtaaatagt tgaccaattt actcgggaat gtcagacttg accctgctat
    17101 gcaatacccc cacttttaca ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa
    17161 gacatgagac tgacatctaa agggcgctat gccgtgaccg caatgcttga cgttgcgctc
    17221 aactctgaag cgggcccggt accgttggct gatatttccg aacgtcaggg aatttccctt
    17281 tcttatctgg aacaactgtt ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt
    17341 ggaccaggcg gtggttatct gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg
    17401 attagcgccg ttgacgaatc tgtagatgcc acccgttgtc agggtaaagg cggctgccag
    17461 ggcggcgata aatgcctgac ccacgcgctg tggcgtgatt tgagcgaccg tctcaccggt
    17521 tttctcaaca acattacttt aggcgaactg gttaataacc aggaagtgct ggatgtgtct
    17581 ggtcgtcagc atactcacga cgcgccacgc acccgcacac aagacgcgat cgacgttaag
    17641 ttacgcgctt aataaaaaga attcagaatc aggccggagt gctaaatact ccgtaaacac
    17701 ggtcgtacat ccagccggta gcctgattcc ttgcattgag tgatgtacgg agtttataga
    17761 gcaatgaaat taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt
    17821 gccgagaaaa tgatgcagtt catgacgatg gacggaacct ttggtaaccc ggcctctcgt
    17881 tctcaccgtt tcggctggca ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc
    17941 gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac
    18001 aacctggcga tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc
    18061 agcaaaaccg aacacaaagc ggtactggat acttgccgtc agctggagcg cgaaggtttc
    18121 gaagtcacct acctggcacc gcagcgtaac ggtattatcg acctgaaaga actcgaagcg
    18181 gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg
    18241 gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt
    18301 gatgcaaccc agagcgtggg caaactgcca atcgacctga gccagttgaa agttgacctg
    18361 atgtctttct ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct gtatgtgcgt
    18421 cgtaaaccgc gtgtacgcat cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg
    18481 cgttccggca ctctgcctgt tcaccagatc gtcggaatgg gcgaagccta tcgcatcgca
    18541 aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac
    18601 ggcatcaaag atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac
    18661 attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac
    18721 ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg
    18781 cgcgcgctgg ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt
    18841 tttactactg aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt
    18901 ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc
    18961 gaatgggctc atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa
    19021 aaagttatcg accattacga gaatccgcgt aacgtgggtt cctttgataa caacgacgag
    19081 aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg acgtaatgaa gttgcagatt
    19141 aaagtcaacg atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttcc
    19201 gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg
    19261 caggcgatca aaaacaccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac
    19321 tgttctattc tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt
    19381 gaagcaaaat aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc
    19441 gcgagtaaat acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag
    19501 aacctccgga tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga
    19561 agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt
    19621 tctggacggt acgcagctgg acttcgtaaa agaaggcctg aatgaagggt ttaaattcac
    19681 caacccgaac gttaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata
    19741 ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc
    19801 gcggcggatc gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc
    19861 cgttatcaac tcgataccca ggcactgagc ctgcgttttc aggatctaca acgtcagtat
    19921 catcctgata aattcgccag cggaagcctg gcggaacaac tcgccgccgt acagcaatct
    19981 gcaaccatta accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg
    20041 ctttctttac acggttttga tctcgccagc gagcagcata ctgtgcgcga caccgcgttc
    20101 ctgatggaac agttggagct gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa
    20161 gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg
    20221 atggttgaac agttagacaa cgaggcgtgg gacgcggcgg cggatacggt gcgtaagctg
    20281 cgttttctcg ataaactgcg gagcagtgcc gaacaactcg aagaaaaact gctcgatttt
    20341 taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc
    20401 gccgcatcag cgtcgtctgg cggctggtat tgacctgggc acaaccaact cgctggtggc
    20461 gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc
    20521 atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc gtactaacgc
    20581 agcgcttgac accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc
    20641 tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg aaaacggcct
    20701 gccgatgatt gaaacggcgg cggggctact gaacccggtg cgcgtttctg cggacatcct
    20761 caaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat
    20821 caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct
    20881 ggcggggttg catgttctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg
    20941 gctggattcc ggtcaggaag gcgtgatcgc tgtttatgac ctcggtggcg ggacgtttga
    21001 tatttccatt ctgcgcttaa gtcgcggcgt gtttgaagtg ctggcaaccg gcggtgattc
    21061 cgcgctcggc ggcgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcgga
    21121 cattcctgat cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc
    21181 caaaatcgcg ctgagcgatg cggactccgt gaccgttaac gttgcgggct ggcagggcga
    21241 aatcagccgt gaacaattca atgaactgat cgcgccactg gtaaaacgaa ccttactggc
    21301 ttgtcgtcgc gcgctgaaag acgcgggtgt agaagctgac gaagtgctgg aagtggtgat
    21361 ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg
    21421 tccaccgctg acttccatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc
    21481 ggatattctg gtgggtaaca agccagacag cgaaatgctg ctgcttgatg tgatcccact
    21541 gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac
    21601 tattccggtg gcccgcgctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc
    21661 tatccatgta atgcagggtg agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt
    21721 tgcgctgcgt ggtattccgg cgctaccggc tggcggtgcg catattcgcg tgacgttcca
    21781 ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggag aaatccaccg gcgttgaggc
    21841 gtctattcag gtcaaaccgt cttacggtct gaccgatagc gaaatcgctt cgatgatcaa
    21901 agactcaatg agctatgccg agcaggacgt aaaagcccgt atgctggcag aacaaaaagt
    21961 agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt
    22021 aagcgccgca gagcgtcagg tcattgacaa tgctgccgct cacctgagtg aagtggcgca
    22081 gggcgatgat gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga
    22141 tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga
    22201 cgaggtttaa tatgccaaag attgttattt tgcctcatca ggacctctgc cctgatggcg
    22261 ctgttctgga agctaatagc ggtgaaacca ttctcgacgc agcgctgcgt aacggtatcg
    22321 agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg
    22381 aaggttttga ctcactgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct
    22441 ggggactgga gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac gaagatttag
    22501 tggtcgaaat cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat
    22561 gggacttaag tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga
    22621 tcttgatccg aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga
    22681 tttcgacgac gacccgcagg catccaacga gaaaatcctc gaagcgattt tgttagtctg
    22741 gctggacgaa gccgagtgaa tgaacgggct gcctgggggt agcccgtttt gctgttaaac
    22801 gaataaggat aactaaaatg acagaagcga tgaagattac catctctacc caacctgccg
    22861 acgcgcgctg gggagaaaaa gcaacttaca gcattaataa tgacggcatt accctgcatt
    22921 tgaacggggc agacgatctg gggctgatcc agcgtgcggc ccgcaagatt gacggtctgg
    22981 gcatcaagca tgttcagtta agcggtgaag gctgggatgc ggatcgctgc tgggcattct
    23041 ggcaaggtta taaaggcccg aaaggcacgc gtaaagtgga gtggccggat ctggacgatg
    23101 cccagcgcca ggaactggat aaccgcctga tgatcatcga ctgggtgcgt gacaccatca
    23161 acgcaccggc agaagaattg ggaccatcgc aactggcaca gcgtgctgtt gatctgatca
    23221 gcaacgtcgc gggcgatcgt gtgacttatc ggatcaccaa aggcgaagat ctgcgtgagc
    23281 aaggttatat ggggctgcac actgtcgggc gcggttcaga acgttctccg gtattgctgg
    23341 cgctggatta caacccaact ggcgataaag aagcaccggt gtacgcgtgc ctggtaggta
    23401 aaggtatcac ttttgactcc ggcggctaca gcatcaaaca gactgcgttt atggactcga
    23461 tgaagtcgga catgggcggc gcggcaacgg ttaccggggc gctggcattt gccattacgc
    23521 gcggactgaa caagcgcgtg aagctgttcc tctgctgtgc ggataacctg attagcggca
    23581 atgcgttcaa gctgggcgat atcatcacct atcgcaacgg taaaaaagtt gaagtgatga
    23641 acactgatgc cgaagggcgt ctggtgcttg ccgatggtct gattgatgcc agtgcgcaga
    23701 aaccggaaat gatcattgat gcggcgaccc tcaccggggc ggcgaaaact gcgctgggta
    23761 atgattatca cgcgctgttc agttttgacg atgcgcttgc cggtcgttta ctggcgagtg
    23821 cctcacaaga gaacgaacca ttctggcgtc tgccgctggc ggaattccac cgcagccagc
    23881 tgccgtctaa ctttgccgaa ctgaacaata ccggaagcgc ggcgtatccg gcaggcgcga
    23941 gcacggcggc gggcttcctg tcgcactttg ttgagaacta tcagcaaggc tggctgcata
    24001 tcgactgctc ggcgacttac cgtaaagcgc cggttgaaca gtggtctgcg ggcgctacgg
    24061 gacttggtgt gcgcacgata gctaatctgt taacggcgta aactaatatg ccggatgcgg
    24121 cgtaaacgcc ttatccggcc tacaaattct gcaaattcaa aaaattgcga atcccttgta
    24181 ggcctgataa gcgtagcgca tcaggcattt gcgccccgaa cgttgaatga attgtggttc
    24241 ttatgtccga aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg
    24301 cgcaccgccc ggcctttttc cgtactctac tggaatccac cgtctgggtg cctggtacgg
    24361 cggcgcaggg cgaggctgtg gttgaagata gtgcgcttga tttacagcac tgggaaaaag
    24421 aagacggcac cagcgtcatt ccttttttca cctcgttaga agcgcttcaa caggcggttg
    24481 aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg
    24541 aaacgctctt ccttaatgcc aaactgccga ccggtaaaga atttatgccg cgtgaaatca
    24601 gtttgttgat tggtgaagag ggaaatccgc tgagcagcca ggaaatcctg gaaggcggtg
    24661 aatcgctgat attatcggaa gtcgcagagc cgccagcaca aatgattgat tcactcacca
    24721 ccttatttaa aaccattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg
    24781 aagaggcaca gcctaattta cttattggca ttgaagccga tggtgatatc gaagaaatta
    24841 ttcaggcgac gggaagtgta gcgaccgata cattacctgg cgatgaacca atcgatattt
    24901 gtcaggtgaa aaaaggggaa aaaggaatta gccactttat taccgaacat attgcgccat
    24961 tctatgaacg tcgctggggt ggttttttgc gtgactttaa acaaaaccgg ataatctaaa
    25021 atatgccgcc ctaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccac
    25081 cagtaaattg tcgccggaga gactccggtt attatttact tgggaaaaca gcgcaccaga
    25141 aacaataagt acgatacaga agcaccgtat cttcattgtg ctgtcgtgat gcgaagaccg
    25201 catcacgaca gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc
    25261 taacggtcgc ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg
    25321 ccgcagtgga taatacacta acagaacaat aataactctc ttttgcttga caaaaagaga
    25381 gttactggcg agtattgtct ccgctgagac gcgcttgcat tactacggta atacaacctg
    25441 ctggaaaggc cgcgaaccgg accagcaaca aataaccgcc tgattttggc ggttttttgt
    25501 tttttaaagg aaagtaaatt tacatgttta agtaatatct tttaccggtt agaattttta
    25561 attttgagca ggatttgttt atatttgtct gaattcggac taataagcca ggttatttta
    25621 ttaccgaaca cggcggaaag ggccggtgtc gataatacgc taaataaaca ataattactc
    25681 tcttttgctt gacaaaaaga gagttactgg tgagtattgt tttactgtga agcacctgcg
    25741 ttgctcatgt aacacaagtc aacctgctgg aaaggccgcg aaccagacca gcaataaaaa
    25801 aacgccaaat tcggcggttt tttattgcct gtaaaatact tatttcactg gctcaaccgg
    25861 taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg tacagtttca cgtttggcac
    25921 atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt acaccagagc cgcagctgac
    25981 gataatcggt ttgtcatagc tgacgccgcg accaaaaaat atcgcatcca gttcatcggt
    26041 cgtttttagt tcgccttcgc gcaccagttc cgtccacgga acgttcaatg caccgggaat
    26101 atgtccgcga cgtaaacctg ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg
    26161 gcgggcatca ataatttgcg ccgtattttc atggcttgcc aataacacat cggttacttt
    26221 caccactgct tcaggattaa acgcggcgtt aaactctcct tccggcagct ctactgcacc
    26281 ttcttctaat agcaggtcat cgcgctgcca gcctgcaagt ccccgttact ttattcgtac
    26341 cccttataat ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtccat
    26401 gatccggggg g
//
LOCUS       DADWTH010000051        25444 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.051, whole genome shotgun sequence.
ACCESSION   DADWTH010000051 DADWTH010000000
VERSION     DADWTH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.051"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            273..614
                     /locus_tag="JNY02_002270"
                     /pseudo
     CDS             273..614
                     /locus_tag="JNY02_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="JNY02_002271"
     CDS             complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="JNY02_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBB9433796.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            2626..3186
                     /gene="pagP"
                     /locus_tag="JNY02_002272"
     CDS             2626..3186
                     /gene="pagP"
                     /locus_tag="JNY02_002272"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBB9433797.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            3361..3570
                     /gene="cspE"
                     /locus_tag="JNY02_002273"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             3361..3570
                     /gene="cspE"
                     /locus_tag="JNY02_002273"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBB9433798.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="JNY02_002274"
     CDS             complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="JNY02_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBB9433799.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            4100..4888
                     /locus_tag="JNY02_002275"
     CDS             4100..4888
                     /locus_tag="JNY02_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBB9433800.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTLGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            5017..5220
                     /gene="tatE"
                     /locus_tag="JNY02_002276"
     CDS             5017..5220
                     /gene="tatE"
                     /locus_tag="JNY02_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBB9433801.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="JNY02_002277"
     CDS             complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="JNY02_002277"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBB9433802.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATERETLLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(6495..7448)
                     /locus_tag="JNY02_002278"
     CDS             complement(6495..7448)
                     /locus_tag="JNY02_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBB9433803.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQSVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="JNY02_002279"
     CDS             complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="JNY02_002279"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBB9433804.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            complement(8449..8712)
                     /locus_tag="JNY02_002280"
     CDS             complement(8449..8712)
                     /locus_tag="JNY02_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBB9433805.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="JNY02_002281"
     CDS             complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="JNY02_002281"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBB9433806.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="JNY02_002282"
     CDS             complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="JNY02_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBB9433807.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="JNY02_002283"
                     /gene_synonym="rodA"
     CDS             complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="JNY02_002283"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBB9433808.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="JNY02_002284"
     CDS             complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="JNY02_002284"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBB9433809.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="JNY02_002285"
     CDS             complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="JNY02_002285"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBB9433810.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="JNY02_002286"
                     /gene_synonym="rsfA"
     CDS             complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="JNY02_002286"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBB9433811.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            complement(15367..15978)
                     /locus_tag="JNY02_002287"
     CDS             complement(15367..15978)
                     /locus_tag="JNY02_002287"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBB9433812.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="JNY02_002288"
     CDS             complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="JNY02_002288"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBB9433813.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            complement(16645..17676)
                     /gene="holA"
                     /locus_tag="JNY02_002289"
     CDS             complement(16645..17676)
                     /gene="holA"
                     /locus_tag="JNY02_002289"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBB9433814.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="JNY02_002290"
     CDS             complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="JNY02_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBB9433815.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="JNY02_002291"
     CDS             complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="JNY02_002291"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBB9433816.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            21089..21571
                     /locus_tag="JNY02_002292"
     CDS             21089..21571
                     /locus_tag="JNY02_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBB9433817.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTELDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            complement(21641..22624)
                     /locus_tag="JNY02_002293"
     CDS             complement(21641..22624)
                     /locus_tag="JNY02_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBB9433818.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            22782..23489
                     /locus_tag="JNY02_002294"
     CDS             22782..23489
                     /locus_tag="JNY02_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBB9433819.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            23486..24913
                     /locus_tag="JNY02_002295"
     CDS             23486..24913
                     /locus_tag="JNY02_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBB9433820.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(24923..25441)
                     /locus_tag="JNY02_002296"
     CDS             complement(24923..25441)
                     /locus_tag="JNY02_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBB9433821.1"
                     /translation="MVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATEL
                     SAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGL
                     GVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYK
                     NAERLLAGLPAH"
BASE COUNT         6357 a         6252 c         6322 g         6513 t
ORIGIN      
        1 atgagctgat ttgtgatatg agtcatttgg tgacagcctg gttgttatcg gttttgaacg
       61 ttttttgaag ctatgattga aatgatttat agtgtttttg tttttcgttt tttcaatgaa
      121 acaaaagcaa ttttttacta tctttcgcgt ttcattggtt aacgcctaaa atttgtacta
      181 caggcttgca agccccacca aatctaatcc catcagatcc cgaataatcc aatgttctcc
      241 cggttttttc gtacttcaag tgaatcaata cactggggca aacgctaacc cgtttccgcc
      301 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
      361 ttaatgccta tgctcgcggt gaacccaaag acgaactgcc gaccggcatt gaccccttga
      421 cgctaaacgc ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg
      481 tggcgcaggc actgaccatc agccgcacca ctgccaggcg ttatcttgaa tattgcgcca
      541 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagacca caacgcatat
      601 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
      661 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
      721 tagcccaaca agcaccggca ccgaggtgcg ttttacgact tcaaatggtg agattttcgc
      781 catcccggca accgcaacga ctacgccaga aacgggtgat agggtacgac ccaggtttga
      841 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
      901 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
      961 cagcatggtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     1021 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     1081 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccatcccgc gataagccac
     1141 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     1201 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc
     1261 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg cgggggcagt
     1321 ggtggtgatt tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata
     1381 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     1441 cgtcgttttg aaggcgaagt caatcagcga catttcggaa gcctgcgccg ccagcaccac
     1501 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagctgctgc
     1561 gccacgactg ataccaacgt ttaccatcac cggaaacagg gttgccatca gcaaaacacc
     1621 cagaccggtt gcggaagaga cggccagtga catcagacaa gcaacaaaat aggcggcaat
     1681 catcagcagg taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat
     1741 atcattcgcg ccgatatggg tcatgtaagc ggcaaagcca cacagcatca taatcatcat
     1801 gccgaggtcg ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc
     1861 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat
     1921 aatcagcaat aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat
     1981 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     2041 aatttttcct gtctccaggc ccaaagtaaa taataaaaac ttcctaaagc ttaaggaaaa
     2101 aatatgccca ataaattggc gatgaatgct gattaaaatc aagaaaaact gccattaaga
     2161 cattgaagtt gctgttttta tacatagata acaaaacgca tatttagcgg ttggcaatta
     2221 tcacataata atgtgtgtaa attgatggtg gattgatgca aatttgttaa ttgcaatggt
     2281 gttagctgcg gctgtgcgct caaaaaataa tctaatatga gcatgggttg acgatatata
     2341 ttatatactt cgttaagatg attgttgtat ctcgttaaaa aaaaataatt tttccttgat
     2401 tgcattttgt catcaaaaaa gacttggttt ttcttttttg actattccca tcgcagaaaa
     2461 cgacgcatca tctttaatcg atgcgcggaa atatttaact tgaacaagcg gaaataaata
     2521 gagcagctat tcagattatt ctttatgttg ggtctattaa ggttatgtta attgtagctt
     2581 tgctatgcta gtagtagatt tttgataaat gttttatggt cacaaatgaa cgtgagtaaa
     2641 tatgtcgcta tcttttcctt tgtttttatt cagttaatca gcgttggtaa agtttttgct
     2701 aacgcagatg agaggatgac aacgtttaga gaaaatattg cacaaacctg gcaacagcct
     2761 gaacattatg atttatatat tcctgccatc acctggcatg cacgtttcgc ttacgacaaa
     2821 gaaaaaaccg atcgctataa cgagcgaccg tggggtggcg gttttggcct gtcgcgttgg
     2881 gatgaaaaag gaaactggca tggcctgtat gccatggcat ttaaggactc gtggaacaaa
     2941 tgggaaccga ttgccggata cggatgggaa agtacctggc gaccgctggc ggatgaaaat
     3001 tttcatttag gtctgggatt caccgctggc gtaacggcac gcgataactg gaattacatc
     3061 cctctcccgg ttctactgcc attggcctcc gtgggttatg gcccagtgac ttttcagatg
     3121 acctacattc cgggtaccta caacaatggc aatgtgtact ttgcctggat gcgctttcag
     3181 ttttgaggca aatgaagttt tagtaacttc tttaaaatca ataactaaaa taagcaacat
     3241 caaaaataac gcgactttta tcactttttg gtaaagttgc actggacaaa gcgtaccaca
     3301 attggtgtac tgataaccga cacagcattt gtgtctattt ttcatgtaaa ggtaattttg
     3361 atgtctaaga ttaaaggtaa cgttaagtgg tttaatgagt ccaaaggatt cggtttcatt
     3421 actccggaag acggcagcaa agacgtgttc gtacacttct ctgcaatcca gactaatggt
     3481 tttaaaactc ttgctgaagg tcagcgcgta gagttcgaaa tcactaacgg tgccaaaggc
     3541 ccttctgctg caaacgtaat cgctctgtaa gatacgtcag caagaattca aaacccgctt
     3601 aatcagcggg ttttttttgg tctttagtgt gcggttgagg ccgaaaacag ccagaatgcc
     3661 agtgcggtca tggcaaaaga ccccagaagg ttgacgaaaa cgttcagtaa tgcccagcca
     3721 aagcggccct cttgtaacaa aaacaccact tctgccgaaa atgttgagaa ggttgttaga
     3781 ccgccacaaa atccggtggt gattaatact ttccacactg gatcaatgtt cgtcatcctg
     3841 ctgaaccagg cgaatcccat tcctatgatg aatgccccaa tcaggtttgc tgccagcgtc
     3901 cccaacggaa tcgcctgatg cagcgggtta aatcgcatac ttaacagcca tctcgctacg
     3961 cttcccgtac caccgccaat aaaaactgct aaaagaagtt gtaacactgc aaaatcctgc
     4021 tatttgattt gtatgagtga taagtgtaac gccgaataat cgtcgttggc gaattttacg
     4081 actctgacag gaggtggcaa tgctggttgc tgcaggacag tttgctgtta catctgtgtg
     4141 ggaaaagaac gctgagattt gtgcctcgtt gatggcgcag gcggcggaaa acgacgtatc
     4201 gctgtttgtc ctgccggaag cattgctggc gcgcgatgat catgatgcag atctatcggt
     4261 taaatcagca cagctgctgg aaggcgaatt cctcggacgt ttacggcgag aaagtaaacg
     4321 taacatgatg acgacaattc tgacgattca tcttccttca acgctggggc gcgcatggaa
     4381 tatgctggtg gcacttcagg caggaaacat cgtcgcccgt tatgccaaac tgcatctcta
     4441 tgatgcattt gccattcagg aatcacgcca tgttgatgct ggtaatgaaa tagctccgtt
     4501 actggaggtg gaagggatga aggtcggtct gatgacctgt tatgacttac gctttccaga
     4561 gctggcgctg gcacaggctt tacagggagc tgaaatcctg gtacttcctg ccgcctgggt
     4621 tcgtgggccg ctcaaagagc atcactggtc aacgttgctt gccgctcgtg cgctggatac
     4681 cacctgttat atggtggcgg cgggggagtg cgggaacaaa aatatcgggc aaagccggat
     4741 tatagatccc tttggcgtca ccattgcggc agcgtcagaa atgcctgcac tcattatggc
     4801 ggaagtgacg cccgaacgtg tgcgtcaggt gcgcgcgcaa ctgcccatct taaacaaccg
     4861 tcgctttgcg ccgccgcaat tattatgatg tttttttact cggcgcttga ttcaccttgt
     4921 tacagattgc tattgtgtgc gcgcgtcgaa tgaccgttaa tattctctgg tttttaaggc
     4981 gcgttctgtt gccggttata tgtcaagaag gtatctatgg gtgagattag tattaccaaa
     5041 ctgctggtag ttgcggcgct ggtcgttctg ctgtttggga ctaagaagtt acgtacgctg
     5101 ggcggagacc ttggagcggc cattaaaggg ttcaagaagg cgatgaatga tgacgatgct
     5161 gcggcgaaaa aaggcgcaga cgttgatctt caggctgaaa agctctctca taaagagtga
     5221 cgtggcgagc aggacgctcc ctcaatatct tgttcgatac aaaaaacccg cttcaaaaag
     5281 cgggtttttt atcagacaga tgtaagtaat tattacagga ttacttaact tccatccctt
     5341 tcgcctgcaa atcggcgtgg taagaagagc ggacaaacgg accgcatgca gcatgggtaa
     5401 agcccatcgc cagcgcttcg gctttcattt cgtcgaactc atccgggcta acgtaacgtt
     5461 gaaccggcag gtgatggcgg cttggctgca aatattgccc cagcgttaac atcgtcacac
     5521 catgacggcg caggtcgcgc attacctcaa taatttcttc attggtttca cccagaccca
     5581 ccatcagacc agacttggtc gggatttccg gatgcgcttc tttaaagcgt tccagcagct
     5641 tcagcgacca gttgtaatct gcgccaggcc gtacctgccg gtaaatacgc ggtacgtttt
     5701 ccaggttatg gttgaacaca tctgggggcg ttgcagtcag aatatccaga gcacgatcca
     5761 ttcgaccgcg gaaatccggc accagagttt caattttgat ttgcgggctt ttttcccgaa
     5821 tggcagtaat acaatccgca aagtgttggg caccgccatc gcgcaggtca tcacggtcaa
     5881 cggaggtgat aaccacataa cgcagcgcca tatcggcaat ggtctgcgcc agtttcactg
     5941 gttcattggc atcaggagct accgggcgac cgtgggcaac atcacagaac ggacaacggc
     6001 gggtacaaat agcgccgagg atcataaacg ttgctgtgcc gtggttgaag cattccgcca
     6061 ggttagggca ggaggcttcc tcgcagacag aatgcaggcc atttttgcgc attgcagctt
     6121 tgatgccctg gatacgtgta gagtccgctg gaagcttgat tttcatccat tccggcttgc
     6181 gcagcagggt ttcgcgctct gttgccacgt ttttaaccgg gataagggcc atcttatcgg
     6241 catcgcggta tttaacaccg cgttccatca caatgggttt actcatagcg tgcgtgttcc
     6301 agttgcgaat tacgaaggaa agcgtttcaa ttcaatagat tgttgtaatt atcaactatt
     6361 tttgaattaa cgactggcag tatatcattg aaacggacct gaaagcagcc aaagcggtcg
     6421 cgaaaatgta aaattgttgt tggattgtgc cattttatcg ttctgcactg tgatctggct
     6481 gtaacaatac tttttcaaaa tgcattacgg ataacatcta ttactccgtg caggatggga
     6541 tcacgcaggc tgaatttgtt gtagtggata gagaaatcta tttgctcctc atttaaggac
     6601 ggaaaaggca gtttttccag cggccagcaa cgactaaaca ggttataaaa acggctggga
     6661 ataatcgcta acatgtcact gttggcaacc agtgcagcga ttgtcaaaat gttgtagctg
     6721 gtgaaattga tttgccggtc cggaaacatc tcttgaactc tctgccgcag accgctgaaa
     6781 ttttgccctt ccggtaacag aagtacatgc gcagcgttgt cgatagtctc tctgtcatct
     6841 tctaaagaga gtagtggatt tcccttgcgg caaattaaca ccatattgtc ggtgaacaga
     6901 acatgatgtt gcaccgtacg attggtgcaa aacatgttat cgatgatgag atcggtttga
     6961 aactgactga gttggttttc cgcgtcgctg atgggcgggt tgcgcagtaa aagctgcgga
     7021 tagtgagttt taatcgcccg atagatgaca ggaaggacca gggctccgac cgagggagtg
     7081 gtcgcaatag ttatcgttcg ttgcttatca tagcttcctt cgatatccag cgcgccaaga
     7141 atggactcaa ggccctgact gatatactca tgtagatgca tcgcaaatgc ggtaggagtg
     7201 acactctggc ctttgcgaat aaacaatggg tcagggaata taacgcgcag tttctgaata
     7261 gactgactga ttgccgaggg ggtcagatta agcactttcg ctgcattaac gatcccttta
     7321 tgtacatata cagcttcaaa aatagtcaga aggttaagat caatatttcg taaggttgta
     7381 aatatttgtg gtttaccttc tgacgacttt ctgcttaaac agggttcaat ttgattatta
     7441 ctatccacgc acttactcca attttattca tggaaaaata atatttaaaa aattacaata
     7501 atcttatgtc taattggaac ggaacgcttt tgctcaccat aatcaactat ttcaataggt
     7561 taatcaatgg ggtgagttac ggagcaaggc gtggcaccgc atttctgcaa gtgataaaga
     7621 aaatatacaa acgcgaagga gatgtaaagc attagcaggt attatgagta atgacccagt
     7681 gtaaattggg ccattgatgt atggaattaa gcggtaatat attcgaagtc cggattgttt
     7741 agtagcgcta aaatattttc cagtaaacgt ggagcaatat tattagtcgt cgcttcgggt
     7801 ttccattgtg atattttagc catttccatt ccggcatacc cacaaggatt aatacgtaaa
     7861 aatggtgaaa gatccatatt gacgtttaat gccagaccgt ggaatgaaca accgcgtcga
     7921 attcgtaaac ccagtgagca aattttcttt tccccgacat agacaccagg cgcgtcagcc
     7981 cgaggatgcg cttctatacc cagttcagcc agggtattca ccactgtttg ctcaagcaag
     8041 gtcaccagtt cacgcacacc gagtttacgg cgtttcaggt taagcaacac atacatcacc
     8101 tgttgtcccg gcccgtgata agtcacctgc ccaccgcgat cgctctggat caccggaata
     8161 tcacccggca ttaaaatgtg ctccgctttt cctgcctgac cttgggtgaa taccggatag
     8221 tgctcgacca gccagatttc atcaagggta ctattatcgc gggtatcggt gaattcatgc
     8281 atagcccggg agattggctc gtaaggctga agaccgagct ggcggacaag aattttatcc
     8341 tgatacaaaa cggcatctcc gtggagaatg agtaaaagtg ggggaaaagt atatcacagc
     8401 gaggagagga gagttacccg accaggagcc gggtaacgga gaagcgagtt acagaaccat
     8461 gcggacaata tcgattttgc ccagttcttc atacagtgtt tcaacctgct cgatatgagt
     8521 ggcgttgata gtgatagata ccgagtggta gttgcctttg ctgcttggtt ttaccgttgg
     8581 ggtgtagtca cctggcgcat ggcgctgtac cacttcaacc acctgatcaa ccagctcagg
     8641 taacgcctgc cccataactt tgtaagtaaa aggagtaggg aattcaagca gttcgttaag
     8701 tttggttttc atgtcagctc cggcgtaacg taattaaata gcaactcccg ccagaaggtg
     8761 ggagtttttt tactgatgct tagtatatgg ggacggaaat tacactttca agtgttcaat
     8821 ttttaaccaa accagtgatg gaacattaat ttaatgtaat caatgatttt gccgaagaag
     8881 ttaccttccg ggatttcttg caacacaacc agcgggcgtt gctcgatcgt tttgccatca
     8941 agctggaagt tgatagttcc gacgacctga ttcttttgca gcggcgcatg caattcactg
     9001 ctgttcagca catagctggc tttcagatct ttcatgcgac cacgcggaat ggtcaggtac
     9061 acgtctttat caacccctaa cgaagcgcga tcagaatcac taaaccaaac cggttctgag
     9121 gcgaactctt tacctacttt cagtgggtta acggtttcga agaaacggaa gccccaggtt
     9181 agcagttttt tactttcggc ttcacggcct ttaaaagtac gtccacccat caccgcagaa
     9241 atcaagcgca tctggccttc agtcgcagaa gcaacaaggt tgtaacctgc tttgtcagtg
     9301 tgtccggttt tgatgccgtc gacattcagg ctgttatccc ataacaggcc gttacggttc
     9361 agctggcgaa taccgttaaa cgtaaattct ttttctttat agatcgagta ttcattcggt
     9421 acgtcacgga tcaatgcctg gccgatcagc gccatatctc gcgcggagct gtactgacca
     9481 tcagcatcca gaccatgtac cgtctggaag tgggtatttt tcaggcccag tgcgttaacg
     9541 tagctgttca tcaagccaac aaaagcgtcc tggctaccag cggcaaaatc ggccatggcg
     9601 acacaagcat cgttacccga ttgcaggtta ataccgcgga tcagctgaga aaccggaacc
     9661 tgcatgcccg gtttgaggaa catcagcgaa gaacctttaa acaccgggtt accggtggcc
     9721 catgcgtcgt tgccgatagt gactaaatca gtttctttaa atttaccggc tttcattgcc
     9781 tggccgataa cgtaactggt catcattttg gtcaggctgg caggatcgcg gcggacatct
     9841 gcgttctgtt cggcgagcac tttgccggag ttatagtcaa tcaggatgta ggactccgca
     9901 tcgatctgcg gtacacccgg gatcatagtt ttgatattca ggtcatcggc atgtgcagca
     9961 gagataaagg ctgtgcaaag agccgtggtg agcgccaggc gcttcatgat acgagcggaa
    10021 aaaatggtat tcatggtctg aactacgaca tccgtgatgg aattaaaaaa gtgccctact
    10081 atagcaaatg cactaccggc aggcatctga ctttccgcgt gactttgtta acgtcattta
    10141 cagaaattga cacatcagat gcctgcttta cgctactgcg cggtagtaat aaatgactgt
    10201 aattgggctt cggtttgcaa acgttgctgc aaggtactgg cttcggcttt gctggcgaat
    10261 gggccaagct ggatccgcca gaccgcgcca ttttgagtta cgcgaccggg gacgccgaac
    10321 ttctgtccca gttgctgttg gtactgttgc gcacgagcct gatcgcttac ggccccgact
    10381 tgcaccataa agttgccgct ggcgctttgc gaggcggctt gcggtgtcac cattgcaggc
    10441 gaggttgcag gcgttgtcga cgaagctgta acaacgggct gtggcgcagg cgtcggttcg
    10501 ctgccttcca gtacaccagg cgctaaggtc gtttgagcgc cgaggaaacc gctactggtt
    10561 accggcgcgc cggtcggatc ttcgcttttt agcgtcgaat tactgaccgg aagaatgtca
    10621 ccctgcgggc cagacactga acttgttccc gcgccaccgc ttaaatcggg aggtgcaggc
    10681 agggcataag tctgtttggc gactgtggta caagccatac caggaccaga aagcgaacca
    10741 tcctgggcga caataatcgg atcgatacga actttggtgt tgtttgacgt gttaagacgg
    10801 tcagctgccg cgcgagaaag cgaaataacg cggtcgttgc cgtaagggcc gcgatcatta
    10861 atgcgcacca cgatcattcg cccgttagcc aggttagtga ttctggcgta gctggggatc
    10921 ggaagcgttg gatgggccgc cgtcagcttt gtcggatcga aagcttcgcc agaggccgtc
    10981 aggttactgc ctggttcggc atcatagatt gccgccagtc ccgcctggct aaatcgagac
    11041 ggatcctgca cgattttgta gcttttaccg tcgcgctggt aatcctgatt tgccgtcgcg
    11101 ttcagtggtt cgaaacgcgg gtccgccccg ctaatttcaa ctatggggcc gttacatacc
    11161 gcaggctgcg gtacacttac cgtctgttgc tgaccatcat cgcttgtaca tgccgcgagc
    11221 attcctgccg cgatgcagat cccgagccac tgcttacgca ttgcgcacct cttacacgct
    11281 tttcgacaac attttcctgt gggtgtggat tgacattaca atcccgaacc cagccatcag
    11341 cacaattagc gccgatcctc cataactgac cagtgggagc ggaaccccta caaccggcag
    11401 aataccgctt accataccaa tatttacgaa gacataaacg aataatatca gcattaagcc
    11461 accagccatg acgcgaccaa aggtggtttg cgctctggcg gctatccaca gcccgcgcat
    11521 gatcagcaga atgtagagag cgagcagaat cagaatgccc actaatccca gctcttccgc
    11581 cagtaccgcg aagataaagt cagtatggcg ttcggggaga aattcaagct gtgactgagt
    11641 gccgtgcagc cagcctttgc cgcgtaatcc gccggagcca atagcaattt tagactgaat
    11701 aatgtgatag cccgcgccga gtgggtctga ttccgggtcc aggagcatca ttacgcgctg
    11761 gcgctggtaa tcatgcatca ggaagaacca cagaatcgga atgaacgccg ctaccagcac
    11821 tactgcgacg ccaatcagac gccagctaag gccagagagg aacagtacaa acagaccgga
    11881 aagcgcaacg aggattgatg ttcccaggtc aggctgtgca gccaccagca gcgtgggcat
    11941 aaatatcagc accagcgcga tgccagtgtt cttcaacgat ggcgggcaaa cgtcgcggtt
    12001 gataaagcgc gcaaccatca gtggtacggc tattttggca atttccgacg gctgaaaacg
    12061 aacaataccg aggtccagcc agcgttgagc gcctttagag atggctccga aagcatctac
    12121 cgccaccagc aaaataatac agatgatata gagatagggg gcccagcctt cataaacgcg
    12181 tggaggaatt tgcgccatca ccaccatgat gaccagaccc atcgcgattt ggccgatttt
    12241 acgctccatc atgccaatat cctgaccgct ggcgctccag ataaccaggg cgctgtaaac
    12301 cagcaatgcc agtaaaatca gcagcattgt gggatcgaga tggactttat cccagaatgt
    12361 ttttttattc ggattatccg tcatgattaa tggtcctccg ctgcggcaac cgctggattt
    12421 tccgcaggca gatcggtgtt gttatcaccc agcataatgt ggtcgaggat ctggcgcatc
    12481 agcgtaccaa ccgccggacc cgcgccgccg ttctccagaa tcatggcgac agccacttgc
    12541 ggattgttgt atggcgcaaa ggcggtcatc agtttgtggt cacgtaaacg ctcggcaatt
    12601 ttgtgcgcat tataggtttc gttcgctttc agaccgaaga cctgagcggt accagatttc
    12661 gccgcaattt tgtacggtgc gctggcaaag tatttatgcg ccgtaccatt agggcggtta
    12721 gcaacaccgt acataccgtc tttcgccagc tcccagtaac cggaatgaat atcgccgacg
    12781 ggcggttcat gcggttgtac ccatggcacc tgtttgccgt cttcggcggt gctcatcagc
    12841 aaatgcggaa ccttcacgat accgtcatta atcaggatca tcagtgcctt actcatctgg
    12901 attggggtcg ctgtccagta accctggccg ataccaaccg gaatggtgtc accctgatac
    12961 cacggttttt taaagcgttt ctgtttccat tcgcgggtag gcatgttgcc ggaacgttct
    13021 tccgccaggt cgataccggt gtaatgacca taaccgaatt tacccatcca ttcggagagg
    13081 cgatcgatcc ccatatcgta ggccacctga tagaagaagg tatccgcaga ttcttccagc
    13141 gatcttgtga cattcagacg cccgtggccc cattttttcc agtcacgata acgtttttcc
    13201 gaccctggta gttgccacca gcctgggtca aacagcgtcg tattgcgcgt gatcaccccg
    13261 gcgcttaatg ccgaaaccgc cacatagggt ttaactgtag acgcgggagg ataaaccccc
    13321 tgtgtggcgc ggttcaccag cggtgtattc gggtcgttca gcaaggcgga ataatctttg
    13381 ctggagatac cgtcaacaaa caggtttggg tcataactag gcgtggaaac cagcgccagc
    13441 accccacctg tacgcggatc ggtaacaacc acagctgcgc ggctacccgc cagcagcgtt
    13501 tcaatatatt gctggagttt gagatccagc gtcaggtaaa tatcgtgtcc ggcttgcggt
    13561 ggtacttctt ttaactggcg gataacacgc ccacggttgt taacttcaac ctcttcataa
    13621 ccggtctgac cgtgcagcac gtcttcatag taacgctcaa tgcccagctt accaatatca
    13681 tgcgttgccg catagttggc cagtttgccg tcattattca ggcgttcgac gtctttatcg
    13741 ttgattttcg acacatatcc gatgacgtgg gtcaacgccg aaccgtaagg atagtaacga
    13801 cgtttatagc ctttaacttc gacacccgga aaacggtact gattgacggc aaagcgagct
    13861 acttgtactt cggtcaggtt agttttcacc ggaatagagg tgaaacggtg tgaacgtgcg
    13921 cgctcttttc ggaatgcagc aatatcgtca tcggtcagat ctaccacgct gcgcaaagcg
    13981 tccagcgttt gctgcacgtt atcgactttc tccggcatca tttctatctg gtagatagtg
    14041 cggttgaggg ccagagggat accgttacgg tcgtagataa tgccgcggct gggcgcgata
    14101 ggcaccagct taatgcggtt ttcattagag cgggtctggt agtcggtaaa gcgaacaatt
    14161 tgcagattat acaggttggc gataagcacg ccggtcagca gcaaaatccc caaaaaggcg
    14221 accagcgccc ggcgcacaaa cagcgcggac tctgccgtat agtcgcgaaa agagttctgt
    14281 agtttcatcc gctgcgtttt ctactcaaag ctcccttatc actcacggtg ataaggatgg
    14341 ttggtggtga tgctccacgc ccggtacaga ctctctgcga ccagcacgcg aaccagcgga
    14401 tgggggaggg taagcgccga cagcgaccag ctctgctcag ccgccgcttt acaggcaggc
    14461 gacaaccctt caggcccgcc aatcagtaga cttacgtcgc gaccatccag cttccagcgt
    14521 tccagctcag cggctaactg cggcgtatcc cagggcttgc ctggaatatc gaggatgaca
    14581 atgcggtttt tgcctgcggc cgccaacatc tgctcaccct ctttgtcgag tatgcgcttg
    14641 atgtccgcat tcttgccgcg tttgccggcc ggaatttcaa tcagctcgaa gggcatatct
    14701 ttcggaaaac gacgcaggta ctcggtaaaa ccggtttgta cccagtccgg cattttcgtt
    14761 cccacggcga caagttgcag cttcacgcat taactccaga gtttttccag ttcatacagg
    14821 cgacggctct cttcctgcat gacatggaca atcacatcgc ccaaatccac gacaatccag
    14881 tcggcgctgt tttcaccttc tacgccgagc ggtaacaggc ccgctgcgcg agactcctgc
    14941 acaacgtggt cagcaatgga cataacatga cggctggacg tacccgtaca gatgatcatg
    15001 cagtcggtga tgctggattt gccttgaacg tctaaggcga tgatgtcctg acctttgagg
    15061 tcatcaattt tgtcgataac aaaatcctgg agtgctttac cctgcaagtt ttccccctgg
    15121 gtgaatcaaa tagataaaaa tggtctgtca gtatacctga accagaggcg atttcgggac
    15181 aattgtcgcc gaatcggctt tcgaaagtgg gctatcatcc caccccgcgc cgcagattgc
    15241 atcgccattt ttgtaaaaca atttctacaa agtcgtgtct ggcggaaaaa gtctggctgc
    15301 ggagaatatc agcctgcccg gggctgtcaa tggtcgttgc ggcttacccg taaaaaaaca
    15361 gaaaagtcat gcattctcga ccccgatggc acggctattg aggacgcgta gcgtcgcgaa
    15421 tttttggttg atatcaatgg cgctccaaca cccctggtca acgcgaaaat gccacataga
    15481 ctcggcaggc atgccaatta aacgagcaat taacagactc agtacgccct gatggctgac
    15541 gattaaaata ttctgatagt gctgatattc actaagcctt gcgataaagc gttccacacg
    15601 ttgcgaaaat gcctgaaatc cttcaccgtt cgtggggata gcatgctgcc agtcattgca
    15661 ccacgcgcta tagttttcgg catcttcttg catgaggtcg cgatgatgtc gcatctccca
    15721 gtcgccaaaa aacatttcgt tgagttcagg gatgatatga acggggagct ggcggtcact
    15781 gagaaccagt cgcgcggtat gctgtgcccg ttccagttca ctgcataaaa ccaggtcaaa
    15841 ggaaacatca tctagcagtg tatgcagatt ttgcgcttgc tcaataccgc gtgcggtcag
    15901 gggtgtgggc gcatgaccgc tgtacagacc atcgacattc gcttgtgttt cgccatgacg
    15961 aattaaccac agtcgcatca tgccctccgt aacgacaggt atcagcgata caagccttgt
    16021 tggttaatgt aagtcaatac cggttccggc aataaatcct cacacgattc accgttttgc
    16081 aaacgttcgc ggatgatggt cgccgagatg ttaaaccacg gcgtttcagc cagataaatt
    16141 ttaccggcag gctgaaggtg aagatcttcc gggttatgtg tcaaatgatc ttccagccat
    16201 tgctggtatt gcggttgcgc catttcaagt gggtaacctg gacgccgaca gacgatcaaa
    16261 tgtgcattgt cgagtatcgt ttcgtattcg taccaggtcg gaaaggtcag caatgaatcc
    16321 tgaccaataa taaacgccag cggcacgtcc ggtccttgtt cctgccgcca ctctttcagt
    16381 gtttgtgcag tgtaagaggg ggcattgcgc tttagctcgc gttcatcaag agtaaataat
    16441 ggcttgtcgg caatcgccag ttcaagcatg tgtttacgct gcacgctgtt cgcttccggc
    16501 tggggacgat gcggaggaac attattaggg atgattgtga cccgcgtcag accaatcaaa
    16561 ttcgccagcg tttccacggg ttttagatga ccatagtgca ccggatcaaa ggtgccgcca
    16621 aacagagcct gtaaagattt catatcaacc gtcgataaat acgtccgcca ggggtttatg
    16681 gcacaacaga agagataacc cttccagctc tgcccacact gactgaccgt aatcttgttt
    16741 gagggtgagt tccgttcgtg tcaggagttg cacggcctga cgtaactgcg gctgacttaa
    16801 gcgatttaac gcctcgccca tcatgccccg gcggttctgc catacccgat gcttatcaaa
    16861 caacgcacgc agtggcgtat gggcagactg gcgtttcagg ttaaccagta acaacagttc
    16921 acgttgtaat gtgcgcaaca aaataaccgg ctcgctgcct tccagacgca gttgctgaag
    16981 aatatgcaac gcgcgcttac tttttcccat caacaaagca tcaacccaat gaaaaggggt
    17041 gaaatgcgcg gcatcattca ccgcctgttc aacgcgcggt aatgtcagtt tgccgtctgg
    17101 ccagagcagc gataaacgct ccagtgcctg agccagcgcc agcaggttac cttcataaca
    17161 gtagcagagc acctgattcg ccgcgtcatc cagttctaag ttgagctgtt ttgcgcgcac
    17221 agcaacccag cggggaagct gagcctgctc cggtgtctga caggtcacct gcacgctgcg
    17281 attcgcaaga gcagtaaacc aggcggcatt ttcttgcgct ttgcttaatt tattaccgcg
    17341 gacgatcaac agcaggtcgt catgcagaag tccggtgagt gtgagaagtt gttcattgat
    17401 cgccgcattc ggtccgtttt ctggtaacaa cagcaatagc gtttgtcgac tggcaaacag
    17461 actcatagcc tggcataacg aaaagatcgc attccagtca gtgttgggat caatggaaaa
    17521 agtgtggtgt tcttcgaatc cttgtgccgc agctacctgg cgaacagcgt cctggctttc
    17581 ctgcaataac agaggatcgt taccaagtaa aagatacgcc gcgcgcagcc cttcattgag
    17641 ctgcgcgcgg agttgttccg ggtacaaccg aatcatcagt tacccagcat ggtggagacg
    17701 cgcgcaggcg ttgccggagt atccgttgtg gtcgacgtct gttcttcgtc ggaacgaata
    17761 tccgcagcac ggatgcttgg cagcttacga atcagctgtt cggcagcacg gtcgtacatc
    17821 tcttttacga tcatgtcttg ttcgttatct ttcgctaacg ccatttgcgg gttatcgaag
    17881 aatgaacgga agactttggc gctaattggg tagatatcac ggccggggat caacacggtc
    17941 gcattcaccg tcatgatcat ctgatactct gctgtttgac cgttacggaa taccgatgcg
    18001 gtatctttcg cgatgctcac tttacccaaa cgcagggatg gaacgtcctt acgcgtggtt
    18061 tctttatcaa gcaactcgac accattcaga cgtaactggt tacgcaccgc acggcttaat
    18121 ggcccgttcg gatcgcctga gtccaggatc atgaccttca tagtggaagg aacctgcgtg
    18181 gtgtcacgca gatgccagcc acacccggcg gtgattaaca ccgccagaga taacaacaat
    18241 gttgccagat atcgcacgct tcctcccgcg cttagccaac gaccagattg aggagtttac
    18301 ctggtacgta aatcacttta cgtacagtaa cgccatcaag atattttgct accagatgtt
    18361 cctggccagc acgttcgcga acctgttctt ccgttgcgtc caccggaacg gtgattttgg
    18421 cacggacttt accgttaacc tgcaccacga ccagcgtgga gtcttccacc atcgcttttt
    18481 cgtcagcaac cggccacggc gcgttgtcga tatcgccttc gcctttgagt tcctgccaca
    18541 gcgtgaagca gatgtgcggg gtgaacgggt tgagcatacg gacaacggcc agcagtgctt
    18601 cctgcatcag agcgcgatcc tgctcgccat cggtcggtgc tttcgccagt ttgttcatca
    18661 gctccataat cgccgcaatt gcggtgttga aggtctgacg acggccgata tcatcggtca
    18721 ctttagcgat agttttatgc acatcgcgac gcagcgcttt ctgatcttca gtcagcgcat
    18781 caacgttcag tgccgcaaca tcaccttttg ctgtgtgctc gtacaccagt ttccagacac
    18841 gtttcaggaa gcggttagcc ccttccacac cggattcctg ccattcgaga gtcatatcag
    18901 ccggagaagc aaacatcata aacagacgaa cggtgtccgc gccgtaacgt tcaaccatca
    18961 cctgcgggtc gataccgttg ttcttcgact tggacatttt gctcatgccg gtataaacca
    19021 gttcatggcc tgccgcatct ttcgctttca cgatacggcc tttctcgtca cgttcaacga
    19081 tagcatcaac cggggaaacc cagttacgtt cgccgttttc gccaacatag tagaaggcat
    19141 ctgccagcac cataccctga cacagcaact gtttcgctgg ttcgtcagag ttcaccatgc
    19201 ctgcatcacg catcagtttg tggaagaagc ggaagtagag caggtgcata atggcgtgtt
    19261 cgataccacc aatgtagata tccaccggca gccagtagtt agccgcttcg gaatccagca
    19321 taccttcttt gtactctggg caagtgtagc gcgcatagta ccaggaggac tccataaagg
    19381 tgtcgaaagt gtcggtttca cgcagcgctg gcataccgtt aacggtagtt tttgcccact
    19441 ccggatctgc tttaatcggg ctggtaatgc cgtccatcac cacatcttcc ggcaggatca
    19501 ccggcagctg gtcgtccggg gttggcatta cggtaccgtc ttccagcgtc accatcggaa
    19561 tcggcgcgcc ccagtaacgc tgacgagaaa caccccagtc gcgcaggcgg tagttcactt
    19621 tacgctcgcc aacgcccatc gcagtcagtt tatcggcgat ggcgttgaag gccgcttcat
    19681 ggtcaagacc gttgaactcg ccagagttga acagcacgcc tttttcagtc agggcttgct
    19741 gagaaagatc tggctcagag ccgtcagccg ccaggataac cggtttgatg ttcaggccgt
    19801 atttagaggc aaactcgtag tcgcgctggt cgtgacccgg aaccgccata actgcgcccg
    19861 tgccgtactc catcaatacg aagtttgctg cccaaacggg aatttcttcg cccgttaatg
    19921 ggtgaacagc tttaaagcca gtatcgacgc cttttttctc cagcgtcgcc atttcagctt
    19981 cggcaacttt ggtgttacgg cattcgtcaa taaaggccgc cagttcagga ttattttccg
    20041 ccgctttctg cgccagcgga tgacccgcag ctaccgccag gtaggtacaa cccataaagg
    20101 tgtccgggcg ggtagtgtaa acggtcagcg tgttgtcata gtcgttaacg ttgaaggtga
    20161 tctccacgcc ttcggaacga ccgatccagt tacgctgcat ggttttaacg gtgtctggcc
    20221 agtgatccag tttatccaga tcgttgagca gctcgtcagc gtaagcagtg attttgataa
    20281 accactgcgg gatctcttta cgttcaactt tggtatcgca gcgccagcag cagccgtcga
    20341 taacttgttc gttcgccagt acggtctggt cgttcgggca ccagttgacc gcagaagtct
    20401 ttttatatac caggcctttt ttatacagct cggtgaagaa tttctgttcc caacggtagt
    20461 attccggcgt acaggttgcc agctcgcggc tccagtcata accaaagccc agcattttga
    20521 gctggttttt catatacgcg atgttgtcgt acgtccacgg tgccggagcg gtgttgtttt
    20581 tcaccgccgc gccttccgca ggcagaccaa acgcgtccca gccgatcggc tgcagaacgt
    20641 ttttgcccag catgcgctgg taacgggcga tcacgtcacc gatggtgtag ttacgtacgt
    20701 ggcccatgtg tagtcgacca gaaggatagg gaagcataga caggcagtaa tacttctctt
    20761 tgctctcgtc ttcggttact tcaaatgtgc gcttctcatc ccaatgaagc tgtactttgg
    20821 attctatctc ttccgggcgg tattgctctt gcatggcagc cagtggtcct gttttcaata
    20881 cggctacaaa tgtagcgttg aggtggtttt tcagatccgc atagcatagc ccaaacgtcc
    20941 gcgtcaaaac agcctttcgc gcactcgacg ttgaaatgat gcccggatta ttcgtacatt
    21001 aatttacaga gtttgtgggc gtattagcaa agcaaggaac aaagaacgtc tattattata
    21061 gtcagttaac gacccgggag atgaaacgat gaacaaggtt gctcaatatt accgtgaact
    21121 ggtcgcgtca ctgaacgaac gcctgcgcaa tggcgaacgt gatatcgacg cactggtgga
    21181 acaggcgcgc gagcgcgtaa taaaaacagg ggagttaacg cgaaccgagc tcgatgagct
    21241 gacgcgagct gtcagacgtg acctggaaga gttcgccatg agctatgaag agagcctgaa
    21301 agaagaatct gacagcgtct ttatgcgggt gattaaagaa agcttgtggc aggagctggc
    21361 agacatcacc gataaaacgc agcttgaatg gcgcgaagtt ttccaggacc tcaatcatca
    21421 tggggtttat cacagcggag aagtggtcgg gctgggaaat ctggtctgcg agaaatgtca
    21481 cttccatctc ccgatctaca caccggaagt gctgacgcta tgcccgaaat gtggttatga
    21541 ccagttccag agacgcccgt ttgagccgta atagtctgat ttcgcatagc aataaagaga
    21601 atatgggcag aatgaaattc cccggccctt gtttttattt ctacagtaaa gcttcagtct
    21661 cacgaatttc ctgactgaca tctctggttc cggatgactg aactttacga tacaggtcga
    21721 gagcctgtcg gagatcgaca ggtcttcctt ctccgtagtg atacataatg gccaggttat
    21781 accaggcagt ggcgtcattg cattcagcgg ctttcgtaaa ccattcaaag gcagcctgat
    21841 aatctttctc aacgccttgt ccgtgtttat acatataacc gatgttgacg taagcgccga
    21901 cgctttcctg agcggcagcc ttcagatacc agtacatcgc ttgcttatag tcctggtcca
    21961 ccccttcgcc agcgttataa tcccatgcaa tttgaaactg ggcgtgccga ttaccctgct
    22021 gtgcgctttt caaataccag aatgcggcga gtgtcttgtt ttgagcaacg ccttctccgt
    22081 ctttataaag atcggccaga ttgtcttgtg cgtaactatg gccttgtaat gcagcttgtt
    22141 tgtaccagaa aaatgccagc gtatagtctt gtgcgactcc gttgccattt ctgtacatcc
    22201 atccaagatt attttgcgca taagacattc cgctctccgc ggctatctga taccaggtaa
    22261 ccgcttcttt atagttgggg tcttctccac gatccagtat ccagccaagg gcgttggaag
    22321 cgaaagtatg gccttgtagg gcagcttttt tcagccagaa gatggcttgc tcattatctt
    22381 tatgacggct tttatcctcg gtatactttt gccctaagga atactgtgct tcacaatgac
    22441 cttgctcagc ggccagcttc agccagtaaa aggccttttc gtcgtccgga gagtcaattg
    22501 cgctatcgcg attataataa aacccaacaa tatattgagc ctcgcaatcg cctttttcag
    22561 cacgttcgat aatctcatct attgataaat tatcgcagca acttgacgta aaaattatta
    22621 tcatttcctt atggttgtag ataaaatata ttatattttt atgtgttcca tttttgcgta
    22681 agccttattt tatgcgtatt atgttgcgtc agaaaagtat aaataactga gtctcgaaat
    22741 gtagtttgca ttcattaaat gaatgtttaa ggataaatta tatggacatg gggtcgcaaa
    22801 aaatattgtt tgcactttcc actccgatgg aaatacgcaa tgaatgctgt ttaccttcgc
    22861 attcatcccc caaaatgtat ttaggtacat gcttttttga cctttcttcc tcatggggaa
    22921 ttgatgaccg tgatgacctg ctgcgcacaa ttcaccgaat gatcgagaac ggtcatgccg
    22981 ctcggttggc agggttttat caccgctggt ttcgttattc tccatgtgaa tggcgtgact
    23041 atcttgctga attaaatgag caaggtcagg cttatgcgca atttgtcgcc agcacagccg
    23101 aatgctgtgg tgagggagga attaaagcat gggattatgt ccggatgggt tttttaagtc
    23161 gaatgggcgt acttaataac tggttgagtg aagaagaaag tctatggatc ctgtcgcgta
    23221 ttcatctacg ggctctacgt tattatagta actggcggca atactttgcc ggttatacct
    23281 ttggtcggca atattggcag tctcccgaag atgatcatct gcctttatta cgcgaattct
    23341 tagcgcgtaa agaatacgac gattccggca atgatatgtt ttatcaatta tttgccagtg
    23401 atgatgcgta ttaccctacc ttgtcctggc aaccactggc tgactacccc acatgcccgg
    23461 aaacgcttaa ggatatgagc gacttatgaa aaattgctgg aagatcctcg atatagagga
    23521 aacgactgac gtcgatatta tccgccgcgc ttatctggcg ctgttaccgt cctttcatcc
    23581 agaaaccgat ccgcagggtt ttaaacaact tcgtcaggcg tatgaggagg cgctacggat
    23641 tgcgcagtcg cctgctaaat ctgtttggca accagaagaa tatgaggtag cagaacatga
    23701 aattctgctc gcctttcgcg cgttacttgc ctctgatagc gaacgttttc tgccctccgc
    23761 ctggcagcga ttcattcagc aattaaatta ttgctcgatg gaagagattg atgaattacg
    23821 ctggtcgctg tgcacaatcg ccatgaacac tgcccattta tccttcgagt gcgtggtgtt
    23881 attagcagaa agattgcggt ggttgcagga ggaaaacgtc ggggaaatag acgaagaaga
    23941 actggaatcc tttttatatg ccattgcgaa ggggaatgtt tttaacttcc agaccattct
    24001 gcatctgccc gttgccgtgc aaaatgacac catcgatttt taccaaatgt tcgctcggat
    24061 ttggtcatcg catccagaat ggctgacatt gtatttagcg caacatcgcg cagtgattat
    24121 ccccgatgat gcaaaactgc acagaaattt actccgctgg tatagcgcaa gccgcctggg
    24181 aatccccgaa ctcctggatt acgcccggtc gtggcgggaa gctgaacctg ataatgaaga
    24241 tgcgcgttat tatgaatacg cacaacgcgt ctattgtgga gaaggcgaaa gcctgctggc
    24301 agaactttgt tactactggc gcgagtatcc ctccacccag gcggatgctt taatgttgca
    24361 atggtgccgt cagcatcggg tcgattatta cccattagtg gtgatgatga ttgaagcgcg
    24421 tgatctggtt aacgatcagg gaaaaccgct actttatgtc cccggcgaca gcgcccgtac
    24481 gcgttttcat ttatacgaaa tactcagcga tgaaaaactc tctgcgctgg ggcgttcact
    24541 ggtcgagatg gttttgcaca aaggacgtaa gccgcggatc tcactcacgc gtgatacaga
    24601 acatccctta tggccattat atttagttgc caaacaatta gtgcaggcca gccaaccgac
    24661 agaagaatca ttaatgccga ttgtgagccg ccttgatgca gaagatcgtt gtccactgga
    24721 agcattaatt attcgtcgat tattaattca ggcggcgaat tttaccggtc aggaaaccgt
    24781 cgagccggag ccgcaaccgc agccaatgcc cgttgacgat ggcgggctgg gctgtctggg
    24841 cgtcatcaaa attattttct acatttttat ctttgccggt ttgataggga aaatactcca
    24901 tctcttcggg tgacagtcga cgttaatgtg cgggaagtcc ggcaagtaat ctttccgcat
    24961 ttttgtaatg ttgtttggcg gccagtttta accatttttg agattcataa tggttaacat
    25021 taataccagt gccatttctg tagatcaaac ccaaagtata ttgagcatga gcataccctt
    25081 gttcagctgc ttccttatac cagagaattg ctttacgggt atcgggtttg actcccaggc
    25141 ctttcaggta gatccacccc agtttgcttt gtgcgcgcgg attttcctgt tctgctgatt
    25201 gctcatacca gtatctcgcc tgtgtataat ccagtggttt accgtcgcta ccatgaagat
    25261 aaaattcgcc aaggcgatat tgcgatgttg agtgaccatg aatcgcggaa agctccgtgg
    25321 ctgccgtggt taacggtaga ctgcctggtg atcttaaata atcaaaggca taattcacca
    25381 tggctttatc acgcaacgtc gttttcttgg gttggctaat acaggtaatt gctgcaacta
    25441 taaa
//
LOCUS       DADWTH010000052        25054 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.052, whole genome shotgun sequence.
ACCESSION   DADWTH010000052 DADWTH010000000
VERSION     DADWTH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.052"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..305
                     /locus_tag="JNY02_002297"
     CDS             <1..305
                     /locus_tag="JNY02_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9433822.1"
                     /translation="GWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRL
                     SQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
     gene            complement(349..4966)
                     /locus_tag="JNY02_002298"
                     /pseudo
     CDS             complement(349..4966)
                     /locus_tag="JNY02_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5059..5706)
                     /gene="rnt"
                     /locus_tag="JNY02_002299"
     CDS             complement(5059..5706)
                     /gene="rnt"
                     /locus_tag="JNY02_002299"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBB9433823.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5809..6216)
                     /gene="gloA"
                     /locus_tag="JNY02_002300"
     CDS             complement(5809..6216)
                     /gene="gloA"
                     /locus_tag="JNY02_002300"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBB9433824.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6297..7394)
                     /gene="nemA"
                     /locus_tag="JNY02_002301"
     CDS             complement(6297..7394)
                     /gene="nemA"
                     /locus_tag="JNY02_002301"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBB9433825.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7431..8030)
                     /gene="nemR"
                     /locus_tag="JNY02_002302"
     CDS             complement(7431..8030)
                     /gene="nemR"
                     /locus_tag="JNY02_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBB9433826.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8133..8372
                     /locus_tag="JNY02_002303"
     CDS             8133..8372
                     /locus_tag="JNY02_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBB9433827.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8421..9317
                     /locus_tag="JNY02_002304"
     CDS             8421..9317
                     /locus_tag="JNY02_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBB9433828.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGMLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9398..9919
                     /gene="sodC"
                     /locus_tag="JNY02_002305"
     CDS             9398..9919
                     /gene="sodC"
                     /locus_tag="JNY02_002305"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBB9433829.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9920..11902)
                     /locus_tag="JNY02_002306"
                     /pseudo
     CDS             complement(9920..11902)
                     /locus_tag="JNY02_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11895..12686)
                     /locus_tag="JNY02_002307"
                     /pseudo
     CDS             complement(11895..12686)
                     /locus_tag="JNY02_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(12689..12925)
                     /locus_tag="JNY02_002308"
     CDS             complement(12689..12925)
                     /locus_tag="JNY02_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBB9433830.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13126..13560
                     /gene="slyA"
                     /locus_tag="JNY02_002309"
     CDS             13126..13560
                     /gene="slyA"
                     /locus_tag="JNY02_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBB9433831.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13607..14074)
                     /gene="slyB"
                     /locus_tag="JNY02_002310"
     CDS             complement(13607..14074)
                     /gene="slyB"
                     /locus_tag="JNY02_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBB9433832.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14348..15457
                     /gene="anmK"
                     /locus_tag="JNY02_002311"
     CDS             14348..15457
                     /gene="anmK"
                     /locus_tag="JNY02_002311"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBB9433833.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15555..15884
                     /gene="mliC"
                     /locus_tag="JNY02_002312"
     CDS             15555..15884
                     /gene="mliC"
                     /locus_tag="JNY02_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBB9433834.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGFSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15943..16599
                     /gene="pdxH"
                     /locus_tag="JNY02_002313"
     CDS             15943..16599
                     /gene="pdxH"
                     /locus_tag="JNY02_002313"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBB9433835.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16728..18002
                     /gene="tyrS"
                     /locus_tag="JNY02_002314"
     CDS             16728..18002
                     /gene="tyrS"
                     /locus_tag="JNY02_002314"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBB9433836.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18061..18930
                     /gene="pdxY"
                     /locus_tag="JNY02_002315"
     CDS             18061..18930
                     /gene="pdxY"
                     /locus_tag="JNY02_002315"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBB9433837.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHVLGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(18974..19579)
                     /gene="gstA"
                     /locus_tag="JNY02_002316"
     CDS             complement(18974..19579)
                     /gene="gstA"
                     /locus_tag="JNY02_002316"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBB9433838.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19685..21187)
                     /gene="dtpA"
                     /locus_tag="JNY02_002317"
     CDS             complement(19685..21187)
                     /gene="dtpA"
                     /locus_tag="JNY02_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBB9433839.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(21798..22433)
                     /gene="nth"
                     /locus_tag="JNY02_002318"
     CDS             complement(21798..22433)
                     /gene="nth"
                     /locus_tag="JNY02_002318"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBB9433840.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKERVDI"
     gene            complement(22433..23128)
                     /gene="rsxE"
                     /locus_tag="JNY02_002319"
     CDS             complement(22433..23128)
                     /gene="rsxE"
                     /locus_tag="JNY02_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBB9433841.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(23132..23752)
                     /gene="rsxG"
                     /locus_tag="JNY02_002320"
     CDS             complement(23132..23752)
                     /gene="rsxG"
                     /locus_tag="JNY02_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBB9433842.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23756..24814)
                     /gene="rsxD"
                     /locus_tag="JNY02_002321"
     CDS             complement(23756..24814)
                     /gene="rsxD"
                     /locus_tag="JNY02_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBB9433843.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24815..>25054)
                     /locus_tag="JNY02_002322"
     CDS             complement(24815..>25054)
                     /locus_tag="JNY02_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBB9433844.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQAN
                     AEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         6185 a         6479 c         6549 g         5841 t
ORIGIN      
        1 agggctggta tgaaatgcgc cgccagcttg agtacaagca gctctggagt ggcggtcagg
       61 tgcttgctgt tccgccagcg tacacaagcc agcgttgcgc gtgctgtggt catacagcga
      121 aagaaaaccg cctgtcacaa agtaaattca gatgccaggt atgtggatat acagcgaacg
      181 ccgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt gcctgtggag
      241 agatggtgca gtcaggccgc tcgttgaagc aggaacccac cgaaatgatt caggcgacag
      301 cttgaacgta gcagggaacc acgtctttca gggcgtggag gatgtcaact atccccaatc
      361 cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc agcgtaaaca tcggcgtttg
      421 ccggaccgga agatcattca cttctgttag cgtaaagcgc agccttggtt cgcgacgcag
      481 tgcggtggtc agcgcctgga aaatctctgg ggcgagtaat tcctcctttt cctgccagac
      541 cagcattttt ttgccgcctt gcgccagata gagtaacaat ttgccgccag aaacgaccac
      601 cagcgcaccc gcccgacgcg ttggtaccag cgctgccgga tgtgcaggcc agggcagaag
      661 atttccccac acattagccg gatcgttggc ggagagcgcc actggtgtat agtggcgtgt
      721 ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata gtcagacgtt cagcgaattg
      781 cgcgccaccc agaccttcta caaaacgacc tcgcataatt cgcccggagt cttccatact
      841 tcgacaaagt gtttgcatcg atggaaaccc gccagggata ttttcggcta tcaccgcctg
      901 acgactgatg atgccgtagc ggtcgagcat attttccgcc agcgccagca tcctttcggt
      961 atcgtttagt ggctccacct gcaataacga ccagcgtcca gccagatttg gtgtgttgta
     1021 agatacccgc ggcgagacgg gttgtgcata gacaggacgc ccacgacgag cccggtgact
     1081 gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg agggcgcgta acggtgccca
     1141 aatatcgctg gtaatgacgc cttgccagac cagcgcccat aacgcctctt gcagtgccga
     1201 ggaatcaacc gattcgccga ttttatcgcg tatccgttgg ctgatttgtt gtgcaaacca
     1261 ggctcctccg tcagccagaa cagcgattat cgcttgctgc agcgccgaac gattcgcctg
     1321 atccgcttcg gcgggagtga acgattctgc ggcatatttc tgtagatgca gtgccaccag
     1381 gccgtcatct tcacccagct tttttttgtc ccgaccagat aaccgctccg gttgccagta
     1441 attcatcgag catttctggc gaatagtcgc gtacgcgggc aggcaggatc tggctttccc
     1501 agagtgaggc gggtaaaccg actccggcaa gctgttcgat cacccgcatc acgccatcta
     1561 cgccctcata aacgcctggc gatgttgagg caaagagcgc cgggctacca tcggtggcgg
     1621 gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt cgtggctgca acgggacgcg
     1681 tcgcttctct ggcggcttgc agcgagcgca aacgcagacg acgaaatact tcatcgctca
     1741 cccagatgtc ttgttgcaga ttcatcacca gaccctgttc acgcagttgc tgaagctgct
     1801 cttcgacaat ggcaataccg agactaaact catgagccag ttgttcagcc gtgaccagag
     1861 catgagcccg gagatagcgc agaaagaggt cgcgaagcgg gtaactgact ctatgtaaat
     1921 aaatctctgg caatgactct ggtagtcgta cgccgagggc atcacgcagc ctggcggcat
     1981 catccataca ggcaagacgc tcctgtccgc taatcatcgc agggaagatt cgttttactg
     2041 caagaagatt ttccagatac gacgcaacct cttcactgct gcctgtatgc cgttgcgcca
     2101 ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc gaacaatcct tcttcacctt
     2161 tcgctcttct gccaggagcc agtcgttgca actcttcttc cacctggcga atgacctgcg
     2221 ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt gcgcagtaac tcgctgtcca
     2281 gcgacagtac ggatgcccgg cgctctgcca gcggggcgtc gctctggtac ataaattccg
     2341 cgacatagcc gaacaataaa cttgtggcga aaggcgaggg cgtagtggtc gttacatcgg
     2401 atatttgaat ttcgccaccg ttcaggcgac gcatcaaacg ttccagtgcg ggaagatcat
     2461 aaacatcttg cagacattcg cgtagggttt cgagaatgac cggaaaatcc ggatatccct
     2521 gagcgatttc cagcaactga ctggcgcgca gccgttgttg ccaaagcggg gtgcgatggc
     2581 ccggcgtgcg gcccggcatt aataatgccc gcgcggcgca ttcgcgaaaa cgggcggcga
     2641 aaagtgccga gctgcctacc gcctcgcgga caatttgcag caacttttct ggttcaaaca
     2701 aaaaaatcgc ggcgtcgggc aatttaccat cagtgtcagg aatgcgcgca acaatgccgt
     2761 catcactggc gaccacagac gcgtcagcgc cccatagggc atgtattcgc ccggcaatcg
     2821 ccagcgccca gggttcatgc acccgtcttc catagggaga gtgcaaaata atacgccagt
     2881 cgccaatttc atcacggcac cgttcgagga ccagatggcg actccccgga acgatgcccg
     2941 tcgcgttgcg ttgctcgtca atcaacccct gaatattggc gttcgtattt tcttctgcca
     3001 gccacggggg aatagtcccg gaaaagaacg cgccatccgc cagtaaatga agaaaatcgc
     3061 cgatcatctc gcctaattca gccggacgtc cgttaccttc accacgccag aaggggagcc
     3121 gggcagaacg acccggagca ggagtcacaa tcacctgatc gcgggtgatt tgctggatcc
     3181 gccatgaggt agcgccgaga gtgataatgt cgttcacccg cgactcatat accatctcct
     3241 catccagttc acccacccgc cgcgaaccgg ccttttcttc accttcgggt aataacacgc
     3301 tatacatgcc acgatccgga atggtgccgc cgctggtaac cgccaacaat tgagcgccag
     3361 gtcgggcggt caatatcccg gtctccctgt tccagaccag tttggggcga aaagcagaaa
     3421 aatcgccaga gggatagcgc ccggaaagca tatccagcgt ggcgtcaaaa acacgtcttg
     3481 gcagatcttt ccacggtgcg gcacggcgta cgcgggagta ccattcgtct acctgtaatg
     3541 catccatcgc cgcggcggca acggtttgct gcgcaaggac gtcgagagga ttatgcggtg
     3601 gtgtcaggtt ttccagcctg cctgcgaaca tacactctac aatgactgcg gaatcgacta
     3661 aatcacgccg ggtacggggg aaaaacagcc ctttagatac accgccaacc tgatgtccgg
     3721 cgcgaccaat gcgttgtaac ccactggcaa cagaaagcgg cgttgcctcc tgaatcacca
     3781 gatccaccgc gcccatatca atccccagtt caagactgga ggttgcgacc acgcagcgta
     3841 attcacccga tttcagcgcc tgttcggtga ttgctcgttg ttctttagag acggagccgt
     3901 ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg gttagaggtt gcgccggagg
     3961 tcgactcgaa atgggccgca tcaacggcga tagacgggga acgctgtaag cgtgcggcgt
     4021 aaagctcatt taatcgtgcc gtcagttttt ccgccagccc gcgtgaatta gtaaagacaa
     4081 tggtcgagcg atggcgcaac acttcatcaa ggatacccgt ttcaatatat ggccagatgg
     4141 agccttcccg gccggcatgg ctgtcttcgc cggtgccgct ggcgaccgat gagacatcat
     4201 ccatattggc gaccggtacg acaattcgta tctgcggatg gcgcattgcg ggcgggttga
     4261 ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac atcgctggct gagcgcacag
     4321 tggcagaaag gccaattcgc tgtgctgagg tgtggagcag cgcatcgagc cgctccagac
     4381 ttaacgccag atgcgcacca cgtttactgc ccgctaccgc gtggacttca tcaataatta
     4441 ccgtttcgac gccgtgtagc gtttcgcgcg cgcgggaggt cagcatcaga tagagtgatt
     4501 cgggtgtggt gatcagaata tccggcggat tacgggtgag tttgctgcgt tcctgtgcag
     4561 gcgtatcgcc agtacggatc cctacgcgaa gattgacttc cgtttcgccg cgccgccgcc
     4621 gttcatcggc aatacccttc aacgggatct gcaagttgcg ctgaacgtcg gtgcccaggg
     4681 cttttatcgg tgaaatatag aggatgcgtg aggttttacg cttatgcgcc tcgcgggtat
     4741 cttcgccgcc ttcgcggaag agccgatcga gggcgtagag aaatgctgcc agcgttttcc
     4801 cggagccagt cggtgcaatc accagcgcat gttcgcctcg cgccgccaca tgccaggttt
     4861 gcgactggac agcggtcggc tgtttaaagg cgcgaagaaa ccagtcgcgg gtcgccggtg
     4921 aaaacacgtc cggcaggagc gatgaagggt ctggattatc tgccattaac gctattcccc
     4981 gtgaatatca tgcttgatgc gtggaatagc ctcactatag atgcctgaat cattgcatgt
     5041 catcaggcat cgactcgatt acacctcttc ggcggcaggt agcggccagc ctcccagacg
     5101 tttccagcgg ttgacgattt cacaaaacag cacagcagtg cgttcggtgt cgtacagcgc
     5161 ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc tggcaagcct ttgacaatac
     5221 ggtttgtccg agtgccagcc cggccagtgc agcagtgtca aaagtggcga aagggtggaa
     5281 cgggttacgt ttcagcgagg cgcgttctgc ggcggccatc ataaagctgt gatcaaaatt
     5341 ggcgttgtgc gccaccataa tggcgcggtt acagccgctc gctttaatac ctttacgtac
     5401 aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc gcgccgcgat cgggatcgtt
     5461 cgggtcaatg ccgttgaagg cgagggcttc tggttgcaaa tttgcgccga caaatggttc
     5521 gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct tgttcatcca ttttcagggt
     5581 gatggcggca atctcaagca gcgcatcggt tttggcgtta aatccggctg tttcaacatc
     5641 gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga ccggtaagtt gagcgttatc
     5701 ggacatctga ttctcttaat acggataaat tgcagcgcgc attatgacaa atattacttg
     5761 tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg gcaggagatt agttgcccag
     5821 accgcgaccg gcgtctttct cttcgattaa ctcaattttg taaccgtccg gatcttccac
     5881 aaacgcgata accgtagtac cgccttttac cggacctgcc tcacgggtca cgttaccccc
     5941 gttttgacgg attttttcgc acgcttcagc ggcgttatct acgctaagcg cgatgtgacc
     6001 ataagcagtg ccgagttcgt atttatccac gccccagttg taggtcagtt caatcaccgc
     6061 ttcttcggtt tccgggccgt agccaacaaa cgccagtgag tatttgtatt ccgggttttc
     6121 gctggtacgc agcagtttca tgcccagcac tttggtataa aaatcgatgg agcgttgcaa
     6181 atcgccaacg cgcagcatgg tatgaagaag acgcatcttt ttatcctcaa aatggctaac
     6241 agattcaaaa tgttgtttta gtatagcggc ggctttacgc cgctcgcaat gttggattac
     6301 aacgtcgggt aatcggtata gccttccgcg ccgccaccgt agaaactttc ggcacgctgt
     6361 gggttaagct cagctttgag ctgcaagcgg gcgaccagat ccgggttcgc aatccagtca
     6421 cgaccaaatg ccaccgcatc aattaaccct ttgccgatca gcgtttcagc tttttctact
     6481 gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg cgcgtacttt ttcgcggaac
     6541 gcatcagtat acggttcacc ccccgcccaa tctggttctg acatatgcag ataagcaatg
     6601 ccgcgtttac ccagttgttc aatcagatac agtgcatcgg cttcttcatt cgggccgtta
     6661 tctgtgttct ggaaagtacc gattggcgaa acgcgaatgc caatgcgatc ggcaccccat
     6721 tcttcaatcc cggcatcgac cacttccagt accaaacgtg cgcgattttc cacgctgccg
     6781 ccgtactgat cggtacgatg gtttgaagaa ggggaaagga actgatgcag caaataaccg
     6841 tgagcagagt ggagctctac cagatcaaaa ccggcttcac gcgcgttagc aatggcctga
     6901 cggaaatcat tgacgatacc cggaatctct tccagttcaa gcgcacgcgg catggatgtt
     6961 tcaacgcgga tcgcccgacc attttcatcg cgcagagaag tacgtgttcc cgcgctaagt
     7021 gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc tggcgtgaga aatgcgtccg
     7081 gtgtgccaca gctgcaaggc catatgacca ttttcagcat gaacgccagc ggtgattttt
     7141 ttccatgcgg caatttgctc cggactatgg atgccaggcg cacctgcata tccttttgcc
     7201 tgggcagaaa tttgcgtggc ttcactaata atcaaaccgg cactggcacg ttggcgatag
     7261 tattccgcca tcaacggggt aggaatgtca cccggttcaa tactgcgcag acgcgtcagc
     7321 ggtgccataa aaatacggtt tgccgccgtg atcgcgccca ctttcagtgg ggaatacagt
     7381 ttttcagatg acataaaggc tcccggtcgt ctggtgataa aaaatgcctg ctaaacggca
     7441 ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt ccagtggctc gaaactgcgc
     7501 gaaattttgg cctgcagatt cgcgccaagc catagtgcgt aaagcacttg tgcctgttgt
     7561 agcggttcgc cacaaaaggt taaacaatgg ttctcacggc cattttccag agcctgcgag
     7621 agcagggcga tcacgccgcg agcaccttta tccatcgcgc tgcgcatatc ttctgacaga
     7681 tcgcacactt cggcagagag ttttactgtc aggcaaccac tgatggttcc atgttggcaa
     7741 aactggttca gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt accttcgccg
     7801 gattgcagca actcagccag tcgctggtga tatgcggcgt aatgacgctc aagcatggca
     7861 acgccaaacg cttctttaga gcgaaagtag tgatagaagg accctttcgg cacttcagcg
     7921 gtttttagta attcgcttag ccccatcccg gtgaatccac gttgcaggca aagttgctcg
     7981 cccgtcgcca agagatgttc gcgagtatca tgttcggtgt gtttgttcat gcgttggagt
     8041 gtagtagacc agtcggtcta gtgcaagcaa agttgctact ttggacatta acagcttcaa
     8101 taatggtaat aatcttatct gaggagggcg aggtggcgga gcaattagag ttctttcccg
     8161 tccagagccc gtgccgggga atttgccagt ctgacgaacg cgggttttgt cgcggctgtt
     8221 ttcgtagtcg ggatgagcgt tttaactgga ataaaatgag cgatggcgaa aaacaagagg
     8281 tgctgcgcct ttgtcggcag cgattaatgc gcaaattacg cgcaaataag ccagcgtcat
     8341 cagacgaacc ggagcaacca tcactctttt gaacctaatc ttgcgtatac tcctctcata
     8401 tctgataaga ggaagcgatt atggttcagc gtattactat tgcgccggaa ggcccggagt
     8461 tttcccgttt tgtgatgggc tactggcgat tgatggactg gaatatgtcc gcccgccagc
     8521 tggtcagttt tattgaagag catctggatc tcggcgtgac caccgtggac catgctgata
     8581 tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc actgaaactg gcacctcacc
     8641 tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg cgtgaagaaa
     8701 acgtcattgg tcattacatc actgaccgca atcacatcat taagagcgcc gaacagtcgc
     8761 taattaatct cgcgaccgat catctggatc tgctgttaat ccaccgacca gacccgttaa
     8821 tggatgccga tgaagtggcg gacgcgttca aacatctgca tcagagcggc aaagtgcgtc
     8881 attttggcgt atcgaacttt acgcctgcgc aatttgccct gttgcaatca cgtctgccgt
     8941 ttacccttgc cactaatcag gtggaaatat ccccggtgca tcagccgtta ctgctggatg
     9001 gcatgctcga ccaactacaa caactgcgtg ttcgtccgat ggcgtggtcc tgccttggtg
     9061 gtggtcgtct gtttaatgat gattatttcc agccgctgcg tgatgaactg gctgtggtgg
     9121 cggaggagtt aaacgcgggc tcgattgaac aggtggttta cgcctgggta ttacgtttac
     9181 cgtcgcagcc gctgccaatt atcggctcag gtaaaattga gcgcgtacgg gcagctgtcg
     9241 aagcagaaac actgaaaatg acccgtcaac aatggtttcg tatccgtaaa gctgcactgg
     9301 ggtacgacgt accgtaagca cttttaggaa tagccgccgt tcaaaaatgt gtcactggtt
     9361 tacacttatt caggaatgca caatgaacgg aggtcctatg aaacgtttta gtctggccat
     9421 tctggcgctg gttgttgcga ccggcgcaca agctgccagt gaaaaagtcg agatgaacct
     9481 cgtcacgtcg caaggggttg ggcagtcaat tggtagcgtc accattactg aaaccgataa
     9541 aggtctggag ttttcacccg atctgaaagc attacccccc ggtgaacatg gcttccatat
     9601 tcatgccaaa ggaagctgcc agccagccac caaagatggc aaagccagcg ccgcggaatc
     9661 cgcaggcggg catcttgatc cacaaaatac cggtaaacat gaagggccag aaggtaccgg
     9721 gcatttaggc gatctgcctg cactggtcgt caataatgac ggcaaagcta ccgatgccgt
     9781 catcgcgcct cgtctgaaat cactggatga aatcaaagac aaggcgctga tggtccacgt
     9841 tggcggcgat aatatgtccg atcaacctaa accgctgggc ggtggcggtg aacgctatgc
     9901 ctgtggtgta attaagtaat tacgaggcca gcgtaccttg cggtggtgct tgctcaagtt
     9961 gcgaaagcga gcagtacagc cgccagacaa ttgccgccag ctcacgggcg gcaggttgat
    10021 gatgacgggc aaggctgtcg caaatccgct gtaattcttc aagtgtggcc gccagtgatt
    10081 tttgctgaac gccacgctca ctcatcactc cccgcaacag tgaaatacag ttatcccgta
    10141 ctcgcgataa cggatcggaa cgcgattccc agtcgcgcaa ttgccagaca acatgagaac
    10201 agttcagcag cactacaccc cagcgtaata accaacggcg agccagcgca tcctggctgt
    10261 tactcaactg actgacgtga tgataagtga gcgattcaaa ttcgctttca ctcagtgttg
    10321 gatggcggct tagctgatcg acaaaatccc ggcgcagcgc gcgaatatgg cggcggcttt
    10381 tacgagcatc cgatcctgga cgcagaatgg cgaacgctag ccaagccagc gcaacgccaa
    10441 cgatttttgc cagattatcg ttaagaaaat cagcaaaatc atacaccggg ggattagtga
    10501 cagcgataaa cgaacccata aaaacaatca gttgccccca cagtgcggca aatttgggca
    10561 tctgcaattt aagaagctgc attgtcgcca gcagtggaaa gagaaacagt aaaaattgcc
    10621 acagatcgct aatctggacc atcaggccaa atttgaccac aaagctgaat agcgaaagta
    10681 acaccagcgt gcgcatcagc agtgacaacg acttaaacgg tgctgcgacg gcggagtaga
    10741 gtacgcagct aattgctgcc agcgttaatg cattggcacc ggcatcccat tgcgatgcaa
    10801 tactccatgc gccaatcatc atcaacgtac aaaatgtacg cagcccgctc cacatagctt
    10861 cggcgttgtc ggtatgacga gccagagcgg atgtccgtgc gagtctggca tgatcatcta
    10921 caccgctttg cagacgatgt aattcctgac tactttgcag ataaaggcgg caaaaataac
    10981 gtagtcgctg ccagaaggcg acgtgccggt agtcggcgac gttggtcggg cgtagcgggg
    11041 cgataatacg tgcgacggtg taagcatctg tttgcgaact ggcgagcgcc gtcagtaatt
    11101 gttcgagaat ttctcgcgtg gcacctggcg gtgagggcca gttgagcaac atacgtcgca
    11161 ggctggagat gacactggtc atacgtaatt gctggtggag tagcgcatta aggcgcgcgt
    11221 tttgctggcg aaaacgatag tggctccaga aagcctggat acgcagcaaa ttcatggtca
    11281 gtatctgccc aatcaccccc tcatgtgctg cacgaatggc atcgtttgtt tcaggctgcc
    11341 agagtaaact ggcatgttcc agtaatcggg cgtgcatgtt tttcaatgca gttaaaaggg
    11401 cagtagcatc ggaactgcta ggcaggatca tcatcatcat gccgccgcac aaaataccga
    11461 ctatcacctc gcaaacgcgc gcctgagcga tatcccacag ctggctggcc tcagtaatat
    11521 taaccatcgg aaaggcgatg atggcagccg tgtagcccgc cagttgaaat gcatacgcga
    11581 cgttattcgt gaagtgcgca caggcctagg tacaaaagcc aagccacgcc gacatgctca
    11641 atagaaaaaa ccacggctca ttgagcgtat gtccggcaag aagcagtgcc gcaatggctc
    11701 ccagtaaact gccagcgatg cgtccgaggc ttttgctgat gacaccgcca acggtgggaa
    11761 agctaaccac tgcagccgag gtcatcgccc aatagggttc atccagattc aaataatagg
    11821 caaccgtcag cgccagacac atggcaatgg tattgcgtaa cgcataacgc cattgcgcca
    11881 gcgtggccct gaaccagcgc aaagcggacg ggaacgcgtt gggcgagacg aacccagggg
    11941 acgttaggtt tcacatccgg aataaggctt gagtcacttt caacgctctg atcataaatc
    12001 gcccgaccga tactggaaac gtgaccctgt aacgttttgt tatcactata gagggtaatt
    12061 tgtgctggtg caccttcgcg gatatggcgc aacttggttt cttcaaaata accaatgacg
    12121 taaaacgagt ggctgtcgac aagggcaaac agcggtttcc cggtgtcagc gtaatcgccg
    12181 atgcgggtag tgaggttagt cacccatcca ctcaccgggg cgcgaatttc cgtttgcgcc
    12241 agttgccatt gtgtctgttt cagcgtcgcc tgtgcggcat taacactggc ctgcatcgct
    12301 ttaacattga ggttagcggt atcgagttct tcggcaaaaa taaaattttg tgagagatga
    12361 cgacgacgat tcgcttcgtt attggctttt gcgaggtcag attgcgcttt cgccagctga
    12421 gcctgtgcgt tcagctcggc gatctgaaag ggcgttttat cgatcgtgag cagaagttcc
    12481 cccgcgttaa ccagttggtt gtcttttata ttcagctcaa caatacgtcc cgacacctgt
    12541 ggggtgatag aaacctgttc tgcgtgtatt ttcccgtcgc gtgtccacgg agattgcatg
    12601 taataattcc acagccacca tccggcaaga accgcaacta tcgctacaat gattgttgag
    12661 aaatacttaa ttgttttaat tgacatagtt accacgcaat cagtattgcc agagcaaggc
    12721 aaacgcaaat cgcaaacagc gataaatcca ttaacaaggg atgccagatg tcaccggcgt
    12781 agatccagcc acgaagcaga cggtgtacga caagccagat gacgaatcca aacgcgaatg
    12841 ctttgaaaaa cggaggaaaa tagacggacg caccgaacac cagatcttgt aagggcaatc
    12901 ctgttgcatt gagcataaac ttcacaaagt agattccttt acgaccgaat atattgcgtt
    12961 agattaataa atattcttta agtgcgaaaa atttacgcgc aatttctgaa aaatatgtct
    13021 gacggtaacc aaatgcagca atacatttgt tttagcaata caattgctgc acactattct
    13081 aaaagccgca taatatctta gcaagctaat tataaggaga tgaaattgga atcgccacta
    13141 ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc tgatagacca tcgcctgaaa
    13201 ccgctggagt taacacaaac ccattgggtt acgttacaca atatccatca gttacctcca
    13261 gaccagtcgc aaattcaact ggcaaaagcg attggcatcg agcagccatc actggtccgt
    13321 actctggacc aactggaaga aaaagggtta atttcgcgtc aaacttgtgc cagcgatcgt
    13381 cgggctaaac gtattaaact gacggaaaag gcagagccac tgatcagcga aatggaagct
    13441 gttattaaca aaacccgcgc ggaaatatta catggcatct ccgcagagga actggagcaa
    13501 ctgattacgc tcatcgcaaa acttgagcat aatatcattg agttacaggc caaagggtga
    13561 aatgaagggg gcgtgtggtc agtaacctga ccacacgcaa acttatttag cgcggagaaa
    13621 cggtcacctg actgccattg ctggccagta cgacacgttg gcccggagag aaacgagtgt
    13681 tgccttgttt ctgtaccacc atgatggtat taccatcgtc tttacgaatt tccagctcga
    13741 caccctgcgt tttgttcatt gcactctgta cgccctgacc agctacgcca cctgcaacag
    13801 cgcctgctgc agtagccaga gaacgcccgg ttccgccacc aacagtattc cccaggaaac
    13861 caccaagaac agcaccgcca attgcaccga taacgttgga atcatcaccg ccctgaatct
    13921 gtaccggacg tacgttaacg atggtgccat agctgacatt ctgtacttgt ttcgcttcag
    13981 aagcggtata aacatcccct gacagggtgt cgttattaac acaaccgaca agagacagac
    14041 ctaccattga aacaaccaat acgcgtttaa tcactgaaaa atctcctgtt caccatgaaa
    14101 cgccacgcaa gcatccctca tggtcaaagt atatggcata tgtgaatcca tggttacatg
    14161 ttctaccaaa tatgagcaaa tcataatgaa agtaggatca accaattata aacaaaacat
    14221 tcatgatgat tattgatttg aagataatgt tttactacca acggtaaaaa tcggtcattc
    14281 cgcatggtat tcacgaaaag tttatgtttg agtggtgcgc aaacgtcaga ttgaaaaagg
    14341 atgaactatg aaatcgggcc gctttattgg cgttatgtca ggcaccagcc ttgatggtgt
    14401 tgatgttgtg ttggcgacaa ttgatgaaca ccgggtcgca cagctggcaa gcttgagctg
    14461 gccgatcccg gtgtctctga aacaggctgt actggatatt tgccagggcc agcagcttac
    14521 actttcgcag tttggacagc ttgatactca actcgggcga ctttttgctg atgcggtcaa
    14581 tgccttgctt aaggaacaaa acctgcaggc gagagatata gttgcgatcg gctgtcacgg
    14641 tcaaaccgtc tggcatgaac cgacgggcgt ggcaccacac actttacaga ttggcgataa
    14701 caatcaaatt gtggcacgca ccggaattac ggttgtcggt gattttcgcc gtcgcgatat
    14761 tgccttggga ggacaaggcg caccgctggt acctgcgttc catcatgcct tgctggctca
    14821 cccaaccgag cgacgaatgg tgctcaatat tggcggcatc gccaatctgt cactgctcat
    14881 tcctgggcag ccggttggcg gctacgatac cggtcctggt aacatgctga tggatgcctg
    14941 gatctggcgt caggcaggta aaccttacga taaagatgcc gagtgggcac gggcgggtaa
    15001 agttattctc ccactgctgc aaaatatgct cagcgacccg tatttctcgc aacctgcacc
    15061 gaaaagcacc ggacgcgaat actttaacta cggctggctg gagcgccatt tacgccattt
    15121 tccgggtgtt gatccccgtg atgttcaggc cacactggca gaactcaccg ccgtgaccat
    15181 ttctgaacaa gttttgttga gcggtggctg cgaacgattg atggtatgtg gtggaggtag
    15241 tcgtaatccg ctactcatgg cgcgtctggc ggtattactg ctaggcacag aagtcaccac
    15301 caccgatgcc gttggcatta gtggcgatga catggaagca ttggctttcg cctggcttgc
    15361 ctggcggacg ctggcgggat taccaggaaa tctgccttcc gtcactggcg caagccagga
    15421 gacggtactg ggggctattt tccccgccaa ctcgtgatat cagaattaac tgaatttttt
    15481 cttatcgtgt ctgccgttac acttggaaga ttaaggaggg tatttgccct ccggaccagg
    15541 aaagtcttcg ggatatgact atgaaaaaac tgttaattat cattttgccg gtccttctct
    15601 ccggttgtag cgcctttaat caactggttg agcgaatgca aaccgatacg ctggaatacc
    15661 agtgtgatga aaaaccgttg acggtcaaac tgaataatcc gcgccaggaa gtcagttttg
    15721 tttacgataa tcaactactg catctcaaac agggcttttc agcctctggc gcgcgttact
    15781 ccgacgggat ctatgttttc tggtcgaaag gtgaggaagc gactgtctat aaacgcgacc
    15841 gcatcgtctt gaataactgt cagttacaaa atccacagcg ttgagatttt tccaggggcg
    15901 gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa ccatgtctga taacgacgaa
    15961 ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag gcgggttacg ccgccgcgat
    16021 cttcccgccg atccattaac cctttttgaa cgttggctct ctcaggcttg tgaagccaaa
    16081 ctggcggacc ctaccgcgat ggtggtcgct accgtggatg aacatggtca gccttatcag
    16141 cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg tgttttacac caacctcggt
    16201 agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta gcctgctgtt cccgtggcat
    16261 acccttgaac gccaggttat ggtgatcggt aaagcagaac gactttcgac tctcgaagtg
    16321 atgaaatatt ttcatagccg cccgcgtgat agccagattg gtgcatgggt ttcgaagcag
    16381 tccagtcgca tttctgcccg cggtatcctt gaaagtaaat tcctggagct gaagcagaag
    16441 tttcaacagg gcgaagtgcc attgccgagc ttttggggcg gttttcgcgt cagccttgaa
    16501 cagattgagt tctggcaggg cggtgagcat cgcctgcatg accgcttttt gtaccagcgt
    16561 gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa aagatgcaaa aatcttgctt
    16621 taatcgctgg tactcctgat tctggcactt tattctatgt ctctttcgca tctggcgaaa
    16681 agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga gattttgatg gcaagcagta
    16741 acttgattaa acaattgcaa gagcgggggc tggtagccca ggtgacggac gaggaagcgt
    16801 tagcagagcg actggcgcaa ggcccgatcg cgctctattg cggcttcgat cctaccgctg
    16861 acagcttgca tttggggcat cttgttccat tgttatgcct gaaacgcttc cagcaggcgg
    16921 gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct gattggcgac ccgagcttca
    16981 aagctgccga gcgtaagctg aacaccgaag aaactgttca ggagtgggtg gacaaaatcc
    17041 gtaagcaggt tgccccgttc ctcgatttcg actgtggaga aaactctgct atcgcggcga
    17101 acaactatga ctggttcggc aatatgaatg tgctgacctt cctgcgcgat attggcaaac
    17161 acttctccgt taaccagatg atcaacaaag aagcggttaa gcagcgtctc aaccgtgaag
    17221 atcaggggat ttcgttcact gagttttcct acaacctgtt gcagggttat gacttcgcct
    17281 gtctgaacaa acagtacgat gtggtgctgc aaattggtgg ttctgaccag tggggtaaca
    17341 tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa tcaggtgttt ggcctgaccg
    17401 ttccgctgat caccaaagca gacggcacca aatttggtaa aactgaaggc ggcgcagtct
    17461 ggttggatcc gaagaaaacc agcccgtaca aattctacca gttctggatc aacactgcgg
    17521 atgccgacgt ttaccgcttc ctgaagttct tcacctttat gagcattgaa gagatcaacg
    17581 ccctagaaga agaagataaa aacagcggta aagcaccgcg cgcccagtat gtactggcgg
    17641 agcaggtgac tcgtctggtt cacggtgaag aaggtttaca ggcggcaaaa cgtattaccg
    17701 aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc ggacttcgaa cagctggcgc
    17761 aggacggcgt accgatggtt gagatggaaa agggcgcaga cctgatgcag gcgctggtcg
    17821 attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac tatcgcctcc aatgccatca
    17881 ccattaacgg tgaaaaacag tccgatcctg aatacttctt taaagaagaa gatcgtctgt
    17941 ttggtcgttt taccttactg cgtcgcggca aaaagaatta ctgtctgatt tgctggaaat
    18001 aatgcattaa gtggaaaggg ggagtgagaa atcactcccc ctggttttta tacagggaac
    18061 atgatgaaaa atattctcgc tatccagtct cacgttgttt atggtcatgc gggtaacagt
    18121 gcggcagagt ttccgatgcg ccgcctgggc gcgaacgtct ggccgctgaa caccgttcaa
    18181 ttttctaatc acacccaata cggcaaatgg actggctgcg tgatgccgcc cagccattta
    18241 accgaaattg tgcaaggcat tgccattgcc gccattgata aattacacac ctgtgatgcc
    18301 gtattaagtg gctatctggg atcggcggag cagggtgaac atgtcctcgg tatcgtccgt
    18361 caggtgaaag cagcgaatcc gcaggcgaaa tatttttgcg atccggtaat gggtcatccg
    18421 gaaaaaggct gtatcgttgc accgggtgtc gcagagtttc atgtacggca cggtttgcct
    18481 gccagcgata tcattgcgcc gaatctggtt gagctggaaa tactctgtga gcatgcggta
    18541 aataacgtcg aagaagcggt tctggcagcg cgcgaactca ttgagcaagg gccacaaatt
    18601 gtgttggtta aacacctggc gcgagctggc tacagccgtg accgttttga aatgctgctg
    18661 gtcaccgccg atgaagcctg gcatatcagc cgtccactgg tggattttgg tatgcgccag
    18721 ccggtaggtg ttggtgatgt gacgagcggt ttactgctgg tgaaactgct tcagggggca
    18781 acgctgcagg aggccctgga acatgtgacc gctgcagtct acgaaatcat ggtgaccacc
    18841 aaagcaatgc aggaatatga gctgcaagtg gtggctgctc aggatcgtat tgccaaacca
    18901 gaacattact tcagcgcaac aaagctctga aatatctcat ttcggcccac aacgacctgt
    18961 gggccgatgg ctattacttt aagccttccg ctgacagcgc gtcttgtact tccggacgtt
    19021 cagccatacg ttgcataaat gccgcaatgt gctctaaccc ttccagattc agtttcaccg
    19081 cgtatgccca gcgcagaacc gtaaacagat aggcatcagc aattgtaaat ctttgcccgc
    19141 agatccagtg ctcatccttc agtgcctcgt tcacatattg cagcttctta tccagctgcg
    19201 cgcgaactgt cggtttgtac tcttccggtg tatctgggcg aaacagaggt gtgaaacctt
    19261 tatgcagctc ggtggcgatg taattcagcc attcgatggt tttatagcgg gaaatactgt
    19321 ttaccggtgc cagcaactgg cggtcgggga cgctgtcggc aagatactgc ataatcgcaa
    19381 cgccttccgt cagcaaagta ccatcgtcca gcagcaatgc aggcacctgc cccttagggt
    19441 taacggaaaa gtaatcgtca ccgttttcga gacgtttttt cattaaatcc acactgacga
    19501 gcgtaaaatc ctttccgctc tcacgcaggg tgatatggga agcgagagag caggcgcccg
    19561 gtttgtagaa caatttcatc ggtaactcct tttgtctgtg gtttaaagta tggtagtgcg
    19621 ttgctgaaca aaaaaaaagc cgctaacgaa aagttagcgg cttgtaaaag agtttccctg
    19681 aattttacgc tacagtagct ttcgccgctt tgtctgcagc gtcatcctgc gtcatgcggt
    19741 gcagtttagg cgcggtcagc agcatcagta ctgcaataac ggcagtagcg acaccaatct
    19801 gcaagaatac gcgaccatag acttccagtg acatcagcgg gtcggtaacg ttatccggca
    19861 cagccatcat acccgcaaca taaccaccaa tcaggtttgc accggcagtg gtcaggaacc
    19921 agctacccat aatgaagccc atcagacgct gcggaacgag ttgtgcaacc attgccagac
    19981 ccagaccaga gatcatcagt tccccgatgc tctgcaggcc atagcttgcg accagccagc
    20041 tgacagacac gatacctgcg tcagacgcga atttcgcacc cagcggcaga atcaggaacg
    20101 caccagaaca catcaccatg ccgattgcaa acttggttgg catcggcagg gtatcaccca
    20161 tcttgttata gatagcggcc agaatcggac taccgatgat gatccagaac gggttcagtg
    20221 cctgatactg ctcaggttct acggccagac ccagaatgga gtgctcaacg ttacgaatcg
    20281 caaagaagtt cagtgacgtt ggcatctggc tgtacagcac gaagaagatg attgcttcga
    20341 gcatcaggat gaaggcaacg atcattttac gacgcgcagc acctttcatc gcgaaagctt
    20401 ctttaccgaa gataaccaca ataccgaagg caacaacgcc cagcgccata cgcgcaactt
    20461 cctgattgtg cagtagccag gtggcaacag cgatcagcgc cacaacacca ataatggtca
    20521 gcaacaggtt acggtagttg actggttcaa agtctggttt tgaaccgtac tgtttaaccc
    20581 agcgttggca gaaggcgaag ttaacgatag tgatcagcag gcctacaacg ctcaacgcaa
    20641 acgcaacact ccagccgtat ttcgcggcca gccacggggt ggctatcata gagaagaaag
    20701 agccgatgtt gacggacatg tagtacatgg tgaatgcacc gtccagacgt gggtcgtttt
    20761 tctcatagca tgtagaaagc agagaagacg ggttagcttt aaacaggccg ttaccgaccg
    20821 caatagccgc catacccata taaacaatac cggcgtcgtg accagaccag gcaaccagag
    20881 cataaccaat cgccagcaca atagcgccga gcataattac gcgtttagta cccagtacct
    20941 tgtcacctaa ccagccgccg atagcgacca gaccataaac cagggcacta aaggaagaga
    21001 aaagggtgat tgaatccgct tcagacatac ccagttgttt aaccaggtaa acagccataa
    21061 ttccttgtag gccgtaataa ccaaaacgtt cccataactc aatcgagaag atgagataga
    21121 acgccttcgg ttgtttgaaa gcgttcaaac tgacgctttc agttggtttt tggtttgcag
    21181 tggacacttt tacctctttt tttacatccc atattaacgg gggggctttc tcgcgttatg
    21241 tctaaaaaac atacgcgcaa ttgttatagt gggaggggaa acggcaggta atgttcacta
    21301 tcttgcactt tcagacaagt cctttgtaat aatctgttac atataactga ggcggggaat
    21361 tcaaccattc cgggaaatgt tatccagcgt taaatttttc aaaaaacgta acggcagatt
    21421 ttttcactgc attgagaggg ggatgatggc tttacggaga tatgttctgc tatttagcgt
    21481 ttttcctagt gagatagtcc agtttctgaa aaatagccag tgtaatgttt tgcaggtcaa
    21541 tatttgttgc gtgtgtaaat ttaaatcatt ttaacctgat tcttgcaatg tttttacatt
    21601 gccacagcat cgtgatcttg atcacgaatg aatagaaaat ttcgctatta aaaaaacaat
    21661 taacctgcat ttttggttat ccgcaaacgg attacctgat gataacgcta agcaatggca
    21721 tcattgacgt taaaaagatg ttttaaaaga ggataaagaa aggttatcaa tggggtaatc
    21781 ggtgttaccc cttttcttca gatgtcaact ctctctttgt attcacaaag atcttcaata
    21841 atacaagagc cacagcgggg cttgcgggca atgcaggtat aacgcccgtg caggatcaac
    21901 caatggtggc agtcgacttt aaactctgcc ggaaccactt tcagtagctt ttcttctacc
    21961 tgttcgacgt ttttccccgg cgcaaattga gtacgattac aaacgcggaa aatgtgcgtg
    22021 tcgacagcaa tagtcggcca gccgaatgca gtgtttaata cgacgttggc tgttttacga
    22081 cctacgccgg gcagggcttc aagcgcagca cgatcttccg gaacctcgcc attatgttgt
    22141 tccagtaata tacggcaggt tttgatgata ttttctgctt tgctgttata aaggccaatc
    22201 gttttgatat aggttttcac cccttcaacg cccagttcaa gcatcgctgc aggcgtattc
    22261 gccaccgggt agagtttcgc cgtcgcctta ttaacactga tatcggtcgc ctgagcggaa
    22321 agcagtacgg caatcagcaa ttcaaaaggc gaactgaaat taagctcggt ggtgggatga
    22381 ggattgttct cacgcaggcg agtgaggatc tccagacgtt ttgctttgtt catcagacat
    22441 tccctgtttc accgtttggc aatgcacgtt cggcagctgc ttcagcacgg cgctttttca
    22501 ttctttcatc aatcaggtat tttcctgcca gcatcagtcc caagccaata aatgcacctg
    22561 gtggcagcat cgccagcagg aaaggggagt cggtgtggaa aatctccacg cgtaatactt
    22621 ttgcccagct acctaacagc gcatctgcac cgtcaaacaa cgtgccattg ccgataattt
    22681 cgcgtagtga acccagcacg aacatggcgc aggttgcgcc cgtaccaatt gaaaagccgt
    22741 ccagtgccga aagcgccgga ccttttttgg cggcgaaggc ttcagcgcgg cccacaacga
    22801 tacagttagt gacaatcagc ggaataaaaa tccctaatga ttgatacagg ccaaaggcgt
    22861 aggcgttgat cagcatctgt acagcgctga ccaccgaggc gatgatcatc acgtaaatgg
    22921 gaatgcggat ctcggctggc gtccagtgac gcagcgtcga aatggtcagg ttggtcagcg
    22981 tcagtaccag cgtagtcgca agtcctaaac ccagagcgtt agtggcagtg gacgtgaccg
    23041 ccaatagcgg acaaaggccg agcagttgga ccagtgcaga gttgtttttc cacaacccct
    23101 gaacaataac gtctttaatt tcgctcacgc tttattctcc acaggcagga agttgagaaa
    23161 gttgtgccgg taacgtctga gcgtacaatc cggcgcgttt taccgcatta accaccgcgc
    23221 ggggagtaat cgtcgcgccg gtgaactggt cgaaatcacc accatctttc ttcaccgccc
    23281 agtgcgcatc atccgcacca ctgatttttt ttcccgcaaa atgggtgagc cagtcagaaa
    23341 ggcgcaattc gattttatcg ccaagccctg gcgtttcgtg gtgctctgtc acgcgcgtgc
    23401 caagtaccgt gccattaaaa tcggctccca ccagcagctg aatcgcaccg gaatagccat
    23461 ctggcgcggt tgcttccaga acggcggcta ccggtttgtc atcctgtttg gcgatgtaaa
    23521 cccgatgctc acctttacct aactctggcg cagttaccag atagcaactc tgtgcgagcg
    23581 cattgttata gcgttcggct ggcagcacct gaccaaataa cgccttttgt tgcagactgg
    23641 cctgttcagc aattgtcgtt ttggtcatct ggttgatggc cgcagttaac cctgtagaac
    23701 ccgctgcaaa tagtgccagt gtaatgccgt gttttcggat agttttcagc atggtttacc
    23761 ctttgcgatg gccgtagacg cgcggacgcg tgtagtaatc gatcagagga accgtgatgt
    23821 tcgccagcag gacggcaaaa gccacgccgt caggatagcc gccgaaactg cggatcaacc
    23881 agactaataa gcccgccagc gcgccgaaca tcagccgacc acgattggtc gtagaagcag
    23941 taactgggtc agtcaaaata aagaatgcgc cgagcatagt cgcaccagac agcagatgaa
    24001 tttgcggtgc tgccagtgtt tctggtgaga acaaccagcc caacgttgcg cataacgcca
    24061 gcgttactaa gaagctgagg ggaatatgcc agcgaatcgc tttctgccat agcaaccaca
    24121 cgccaccagc cagccaggcg agatttaccc attgccaacc agcgcccgcc agaataccgc
    24181 tgtagatcgg atattgcata atctgttcaa ccgaatgacc tgcgcggaca gaggttttga
    24241 atgtatccag cggtgtcgcc tgactaatgc catcaatacc taagcgtagc gtattcatat
    24301 caccaccact ggcggtatga ccgctgaaaa taacctgtat ggcgtcgata aaaccaggga
    24361 tgttgaccgc aatttcatgt ggcggtaacc agctggtcat ctgcaccggg aaggagatca
    24421 gtaagaccac ataaccaatc attgccggat taaacgggtt ttgccccaga ccgccataca
    24481 actgtttagc gatgatcacc gcaaacaccg tacccagtac gaccatccac catggcgcta
    24541 gggggggaat acttaccgcc agcaataagc ctgtcagcaa tgctgagtta tctttcaacg
    24601 ttgcggctac cgactgcttg cgtagtttga gtacgagagc ttcggctaac agagcactga
    24661 ccgacgccaa caggatctga acgagagtac cccaaccaaa aaaccacagt tgcgctgcga
    24721 ttcctggcac ggctgcgagc aacaccaaca gcataatgcg cgatgtctgg cgctggttat
    24781 gggtataagg ggagctagct attctgaata ccatttagtc ctcgtttaca accttttgct
    24841 gggcggcttt tttggcctga acgcgggcaa tagccgcggc aactgcggct ttgcgcggat
    24901 cgacctgttc ttctggttcc gcattagcct gttgctgttc cagcttgcgt gctttggcgc
    24961 gggcaatagc ggcttcgacg gcagctttgc gcggatcaac ctgttcttct ggctccgcat
    25021 tggcctgttg ctgttccagc ttgcgtgctt tggc
//
LOCUS       DADWTH010000053        24860 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.053, whole genome shotgun sequence.
ACCESSION   DADWTH010000053 DADWTH010000000
VERSION     DADWTH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.053"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..352
                     /locus_tag="JNY02_002323"
     CDS             71..352
                     /locus_tag="JNY02_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBB9433845.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1394
                     /locus_tag="JNY02_002324"
     CDS             318..1394
                     /locus_tag="JNY02_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HBB9433846.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMADWIERYKTILIRRKASRNTYKTRVN
                     QLVTIKENLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGILFSENPPTFHEIRSLAGRLYKEICGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1787..2128)
                     /locus_tag="JNY02_002325"
     CDS             complement(1787..2128)
                     /locus_tag="JNY02_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBB9433847.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2141..3013)
                     /gene="copD"
                     /locus_tag="JNY02_002326"
                     /pseudo
     CDS             complement(2141..3013)
                     /gene="copD"
                     /locus_tag="JNY02_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="JNY02_002327"
     CDS             complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="JNY02_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBB9433848.1"
                     /translation="MASTARSLRYALTILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3530..3760
                     /gene="holE"
                     /locus_tag="JNY02_002328"
     CDS             3530..3760
                     /gene="holE"
                     /locus_tag="JNY02_002328"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBB9433849.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3862..4518
                     /locus_tag="JNY02_002329"
     CDS             3862..4518
                     /locus_tag="JNY02_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBB9433850.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4542..5204
                     /gene="exoX"
                     /locus_tag="JNY02_002330"
     CDS             4542..5204
                     /gene="exoX"
                     /locus_tag="JNY02_002330"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBB9433851.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="JNY02_002331"
     CDS             complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="JNY02_002331"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBB9433852.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQHWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7470..8129)
                     /locus_tag="JNY02_002332"
     CDS             complement(7470..8129)
                     /locus_tag="JNY02_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBB9433853.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8456..8812)
                     /locus_tag="JNY02_002333"
     CDS             complement(8456..8812)
                     /locus_tag="JNY02_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBB9433854.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8879..9169)
                     /gene="yebG"
                     /locus_tag="JNY02_002334"
                     /pseudo
     CDS             complement(8879..9169)
                     /gene="yebG"
                     /locus_tag="JNY02_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9303..10481
                     /gene="purT"
                     /locus_tag="JNY02_002335"
     CDS             9303..10481
                     /gene="purT"
                     /locus_tag="JNY02_002335"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBB9433855.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10536..11177)
                     /gene="kdgA"
                     /locus_tag="JNY02_002336"
                     /gene_synonym="eda"
     CDS             complement(10536..11177)
                     /gene="kdgA"
                     /locus_tag="JNY02_002336"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBB9433856.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGVGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11214..13025)
                     /gene="edd"
                     /locus_tag="JNY02_002337"
     CDS             complement(11214..13025)
                     /gene="edd"
                     /locus_tag="JNY02_002337"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBB9433857.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13260..14735)
                     /gene="zwf"
                     /locus_tag="JNY02_002338"
     CDS             complement(13260..14735)
                     /gene="zwf"
                     /locus_tag="JNY02_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBB9433858.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15074..15943
                     /gene="hexR"
                     /locus_tag="JNY02_002339"
                     /gene_synonym="yebK"
     CDS             15074..15943
                     /gene="hexR"
                     /locus_tag="JNY02_002339"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBB9433859.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16071..17513
                     /gene="pyk"
                     /locus_tag="JNY02_002340"
     CDS             16071..17513
                     /gene="pyk"
                     /locus_tag="JNY02_002340"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBB9433860.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17644..18615)
                     /gene="lpxM"
                     /locus_tag="JNY02_002341"
                     /gene_synonym="msbB"
     CDS             complement(17644..18615)
                     /gene="lpxM"
                     /locus_tag="JNY02_002341"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBB9433861.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18735..20057)
                     /gene="mepM"
                     /locus_tag="JNY02_002342"
     CDS             complement(18735..20057)
                     /gene="mepM"
                     /locus_tag="JNY02_002342"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBB9433862.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20073..21005)
                     /gene="znuA"
                     /locus_tag="JNY02_002343"
     CDS             complement(20073..21005)
                     /gene="znuA"
                     /locus_tag="JNY02_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBB9433863.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21084..21839
                     /gene="znuC"
                     /locus_tag="JNY02_002344"
     CDS             21084..21839
                     /gene="znuC"
                     /locus_tag="JNY02_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBB9433864.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21836..22621
                     /gene="znuB"
                     /locus_tag="JNY02_002345"
     CDS             21836..22621
                     /gene="znuB"
                     /locus_tag="JNY02_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBB9433865.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22768..23778)
                     /gene="ruvB"
                     /locus_tag="JNY02_002346"
     CDS             complement(22768..23778)
                     /gene="ruvB"
                     /locus_tag="JNY02_002346"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBB9433866.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23787..24398)
                     /gene="ruvA"
                     /locus_tag="JNY02_002347"
     CDS             complement(23787..24398)
                     /gene="ruvA"
                     /locus_tag="JNY02_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBB9433867.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24537..24602)
                     /gene="yobI"
                     /locus_tag="JNY02_002348"
     CDS             complement(24537..24602)
                     /gene="yobI"
                     /locus_tag="JNY02_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBB9433868.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24673..>24860
                     /locus_tag="JNY02_002349"
     CDS             24673..>24860
                     /locus_tag="JNY02_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433869.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITT"
BASE COUNT         6165 a         6295 c         6107 g         6293 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcaga ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg tgacaataaa agaaaattta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtgg attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcctgt tcagtgaaaa cccaccaact tttcacgaaa
     1261 tcagaagcct ggctggacga ttgtacaaag aaatatgtgg agaagaattt gctcagcgtc
     1321 tacttggcca cacatcggag aagacaacaa aaatgtatct tgatgagaga gaaaaaacgt
     1381 acttactgct ctgattttaa cgtaaatgga ttgttaaatg tattttggtt gtgatataac
     1441 caaaaaagac cggaatacag aaattcgagt aaatttcggg gaatttcggg gagacgtttg
     1501 caactaattg attttaaata caattaaaaa aagaccgaat acgattcctg tattcggtcc
     1561 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
     1621 tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
     1681 caataaaaag cgcggtggtc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga
     1741 actgaggcgt tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt
     1801 cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa
     1861 tctgaatcac cgaaatgggt tgggcattgg tcttcccact ggcaacttcc ttttgtgcga
     1921 tatcgtttaa aggatactgc acgagggtac ttgggttgat gacatacaaa gcattacccg
     1981 gtcggcaagt cagcattacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa
     2041 aacgactcac ggttatcacc tgtggagccg ccagcgccgc tgcagaactg gtgagtaaca
     2101 gaaacgccag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg
     2161 caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc
     2221 acacaaacca gggtgccgca cgatcttcat cctgcctcat gcgcggtaca agaacataac
     2281 gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt
     2341 ggccctagta agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc
     2401 cgcttgccag tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct
     2461 gaatagcctg gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta
     2521 atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt
     2581 tcgctgttac cccttcattc agcgtcgcat gccccactcc cgccagcaga ataaattgcg
     2641 cggtagtgag cataaacaac aaacgtggca tattccgcgg ttgcataagg gcgacgatca
     2701 acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa
     2761 cagcctgcca aatgtttggc gaaaatacat ctgtccatcc cgttcccatc aacccgccct
     2821 gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac
     2881 gcagagaacg cttcgtcagc aagcgacgaa tcattaaggg ggccagccag gcaccataca
     2941 ttgcaaagcc aaagaccagc atcaacgagg tgaaatggat aaatcgcagc gcgatccagg
     3001 taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt
     3061 ccaccgaaac aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg
     3121 gaacaatcag ttgcttttgg tcctgctcat tccgcttcgc tggtaatgtt ttaatatttt
     3181 cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttcaacgcct tccgagaagt
     3241 ttaaagtgat tgcctgcggg gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg
     3301 ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgtcagcg
     3361 cgtagcgaag ggagcgtgca gttgaagcca tattatctat tcctttttgt aataactttt
     3421 ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc
     3481 caggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa
     3541 tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg
     3601 ggtggctttt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca
     3661 gccagaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt
     3721 caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact
     3781 ttcttacata acgcctgcta aattatgagt attttctaaa ccgcccccat aatttgcagt
     3841 cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct
     3901 cgtaaaacct cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc
     3961 aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg
     4021 cgacgtgccc ttcctgcccc acccgatctt tcactgttag acccgctttg ttatgccgca
     4081 acgacctggc gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt
     4141 cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggaactacca tcagagtcat
     4201 ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accgcaaggt
     4261 atggatatgg acccgacctg ttcactgttt acgacagggc aatgtctggg agaacccgac
     4321 ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat tgccgtgctg
     4381 atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc
     4441 gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat
     4501 atcattccat tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata
     4561 cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg
     4621 gaaaaatcgt caaccccatg agcctcctgg tgcgccccga tcgtcctatt agtccacaag
     4681 cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg
     4741 tgatcccaca ctattacggt agtgaatggt atgtcgcgca caacgccagc tttgaccgcc
     4801 gcgtactgcc tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt
     4861 ggccagggct caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga
     4921 caccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc
     4981 ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac
     5041 gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg
     5101 ttgccgaacg cgatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc
     5161 cggagctgcg tttaacactg aaacattatc tggaaaatac ttaatccgca ggcgtagtgg
     5221 gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg
     5281 atttaaagcg gccagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa
     5341 gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatgtt
     5401 gcacctgaga atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga
     5461 cgttgtcata cgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcggatttc
     5521 cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa
     5581 catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg
     5641 caacgcccat caacatgccc cccgcacttc cgcccatcgc ataacagagc gaaggagagc
     5701 catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct
     5761 tcttcagaaa tttaccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat
     5821 ggacaatggc gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg
     5881 catcaatact tgcgccgtaa gaaccatagc catacaccag caacgggttg tgtcctttgc
     5941 gaaaatgttt gcgatgatag accagcgaaa ccggaacttc gacgccatca cgggcgacta
     6001 tccacaggtg ttcactgcgg taattcgccg catcaaaacc gggcacttct gtttgtttta
     6061 atacacgacg ctcaccggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg
     6121 aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca
     6181 cataggccgg atcatcaaag gcaatgccaa tgacttcccg ggtcttgcgg ttaatttggc
     6241 gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa
     6301 acccttccag catgatgttt tcacgtggcg gaattaactc ttcccactgt tgctcatcac
     6361 gcatacgggt acggtataag ccaaagtttt tgccgtggcg attggaacgc agataaaaac
     6421 gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg
     6481 gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca
     6541 gatgaatgac gacatagtgc ttcgacgtcg ttttatgcag gctgacataa taggtatcgt
     6601 ctttttcttc gtagatcagt gtatcttgcg atgctggcgt accgatggcg tgacgccaga
     6661 cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat
     6721 ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttacctgttt
     6781 ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca
     6841 taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg
     6901 cgcgcttgtt ggcatcgagc aatgtttccc actcatccca ctcttcgctg aatgccgatt
     6961 gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat
     7021 tttttatgta gggcgcagaa acttctcttt gcggaatgcg gtcgatgatt tcctttaaga
     7081 tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt
     7141 gcaggtagtc caggacttct ggctgagaac gcgtatcgtc ccgcagccag tagtaattat
     7201 cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca
     7261 tgttattgtt ctttctggtt gaaacatctt ataagggtgg caaaactcac cggggatgca
     7321 agcgaaacag ggtcgtcatt gcttagatga tgacaggtaa tggcgcggat atcgaatgtt
     7381 atgcagacag agaaaatcag cctgttcaaa tggctgtgcg gttctggata gcccgaaata
     7441 gtcaacttca ggctatccag agagcggaat tattccgcca aagtgcgttt ttgctgttcg
     7501 agatcgcgtt caatgccttc acgaacatcc tgggggattt tcagcgcgtc acccagtgca
     7561 ttcaggtaac tgcgttccat aaaatggtca atatcaatag ccgcgcagct caggaaatag
     7621 atttccagcg cctcttcttc attgcggacc ccggtagcca ggcgttgtgg atccagcggt
     7681 tgttcgattg cctgctcaat gagtacacgc ccctgctctt ccacgccggc ttcacgcaat
     7741 tgctggtcga ttgccgcacg ttcattggca tcaatatgac catcactttt agcggcaaag
     7801 accagcgcaa ggatcaaacg ttccgtacgc tcatccagcg gcgtactttg cgcgccaaac
     7861 tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt atttattcca cagcaccgta
     7921 cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc catatttcgt aagaagttta
     7981 cgtgctgatt tatttgcgac cagcagccca gccagtccgc ctaatgcgcc tggcactaac
     8041 agtttgccca atccctgatc cgcagacgag gaggtagaag aactgctttg cccaagcagg
     8101 gattgcagtt gatttaacca gttagccata tttgctcctc aataaccatt tttagtgcct
     8161 gtagcatagc ggatgaggat gtcagaaaat gtatatcgag acaaaagatg cgcaaataag
     8221 cggggcggta ggccgttatt caaagaaatt cgcgccaggt aaggtattta ctgtatatca
     8281 ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct
     8341 gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg cgttctggga
     8401 cggacaaggc gctcgcgccg catccgccaa cggtcaatcg aattatcaga agccattaac
     8461 gccgctgata ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg
     8521 cacttttacc acgcacggtt agcggtactg accatttatc atctttaccc tgaatgtcct
     8581 gcaaactgac ccaggccacg ggatcggcct gaccgacaat tttttgatca tctgcccagc
     8641 gcgccacgcg attttgttga taatcacgtt ttacgctcgc ggcaattccg gtagcatcca
     8701 gaccttcaca ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg
     8761 atgcgcaggc agaaaccaac aaccgcccta aaaacgcccc tctttttttc atgtttttct
     8821 ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc
     8881 aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgaccagt ggggacggta
     8941 atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa
     9001 gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagctag gtgtcaagca
     9061 gatccgccgt atcaagcatt ttgtcataag catcggcttc ctttttgctg gtaaacgaca
     9121 ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct
     9181 cataataact gtgattttat acagtatatt tcttttcggt tgagaaatca acatcagcaa
     9241 taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg
     9301 ttatgacgtt attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct
     9361 ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg
     9421 ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta
     9481 atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg
     9541 tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga
     9601 atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc
     9661 tggcggcaga agagctgcag ctgcccactt ccacttatcg ttttgccgat agcaaaagcc
     9721 ttttccgcga ggcggttgcc gccattggct atccctgcat tgtaaaaccg gtgatgagct
     9781 cttccggcaa ggggcagacg tttattcgct ctgcagagca acttgctcag gcatgggaat
     9841 acgctcagca aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt
     9901 ttgacttcga aattaccctg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac
     9961 cagtaggtca tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga
    10021 gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg
    10081 gttatggtct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg
    10141 tctcccctcg tccacatgat accgggatgg tgacgttaat ttctcaagat ctctcagagt
    10201 ttgccctgca tgtacgtgcc ttcctcggac ttcctgttgg cgggatccgt cagtatggtc
    10261 ctgcagcttc tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg
    10321 tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg
    10381 gcagccgtcg tctgggggtg gtactggcta cttcagagag tgttgttgac gccattgaac
    10441 gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaacgctac
    10501 aaaaatgccc gatcctagat cgggcatttt gacttttaca gcttagcgcc ttctacagct
    10561 tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc
    10621 agccaggaac caccgatgca cagcacgctt ttcagtgcca ggtagtcacg gtagttagcc
    10681 ggagaaatac cacccgtcgg gcagaaacgg acctgggaga acggacccgc gatcgcctgc
    10741 agggctttca cgccgccgtt agcttcagcc gggaagaatt tgaactcttt caaaccgtag
    10801 tccataccca gcatcagttc ggaaacagtg ctgatccccg gaatcagagg aatagtccct
    10861 tcggtagcag ctttcagcag cggctcggtc agacccgggc taattgcgaa ctgtgcaccc
    10921 gcttcagtga cttctgccag ctgctgtgga ttcagcaccg taccgacacc cacaatcgct
    10981 tcaggcactt ctttggcgat agcacggata gcgtcaactg cacactcggt acgcagagtc
    11041 acttccagaa cgcgcacccc accagcaacc aacgcttttg ccatcggcac cgcgtgttcc
    11101 agttttttta ccacgataac cggtacaacc gggccggtgg tcaggattga ttctgcactt
    11161 gttttccagt ttttcatcag agttttctct cgcctgatta caaatttgtc gtcttaaaaa
    11221 gtgatacagg ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc gctgaataat
    11281 tcacgtcctg ttctcacgcg tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc
    11341 agttccgctt cgtctaccag cagtgtcagt tcgcctgtct gtccattcac acgaatgatg
    11401 tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg cttctggtgt tacgtggata
    11461 gctgacggca ctttacctga agcgccggag agtcgtccat cggtaactaa cgcaattttg
    11521 aaacaccggt ccaataatac accaagtggc ggcatgagtt tatgtaattc tggcattccg
    11581 ttcgcttttg gcccctgatg acggacaaca acgacacaat cgcggtccag caaacccgct
    11641 tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc
    11701 tggttctcaa ccggcacggc agaggttttc ataaccgcac ggcccaggtt accgcttaac
    11761 acttttgtcc caccatgatg agagaaaggt tgttcgaagg aagcgatcac attgctgtcg
    11821 agtgattttt ccgccccttc ccgccagtcc agttcaccat tattcagcca tggttcaagg
    11881 gtataacgaa acagaccaaa gcctgccacc gtattgacat cttcatgcag caggcctgct
    11941 ttgagcagtt cacgcaccag aaccggtacg ccacctgccg cctggaagtg gttaatatcg
    12001 gccggaccgt tcgggtagag acgtgccatc agcggtacaa catcagaaag gtcagagaag
    12061 tcatcccagt taatctgaat accggccgcg cgcgccatcg ccaccaggtg catggtgtgg
    12121 ttagtggaac caccggtcgc caacagtgca acgataccgt tcaccaccac tttctcatcg
    12181 atcatcttac cgatcggcat ccattcatta ccattaccgg tcatgcgtgt aacctgacgc
    12241 gcagcggcag cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa agaagagcct
    12301 ggcaattgca tccccataaa ttccaccacc atctggttgg tgttggcagt accgtagaaa
    12361 gtacatgttc ccggcgcatg gtaagacgcg gcttctgact ccagtaaggc catgcggtcc
    12421 actttacctt cggcataaag ctggcgaata cgcacttttt ctttatttgg caaaccgctt
    12481 gccatcggtc cagacggcac aaacaccgca ggcaaatgac caaacgacag ggctgccatc
    12541 gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta
    12601 tgggacagcc ccaccgccgc agacatcgct atcacttcgc ggcttagcag cgacaattcc
    12661 attccatcct gcccctgggt gacaccatca cacatcgccg gaacaccgcc cgcaacctga
    12721 ccaaccgcat tcgcttcatg cagggcttta cgaatgattt ctggatagtg ttcataaggc
    12781 tggtgcgcgg agagcatgtc gttataggag gtgatgatgg cgatattgtt acgcaacatg
    12841 cttttcaaag aggctttgtc ttctggctgg caggcagcga aaccgtgtgc caggttaccg
    12901 catgccaact gcgaacgatg aacggtcgac gttttcgctt gttctatccg ggcgagataa
    12961 gcagagcgag tctcgcgcga acgttcaatg attcgatttg ttacgcgtaa caattgtgga
    13021 ttcataaagg ctcctgaaat tgagttgtca gagcaggatg attcacaacg cgtttcattc
    13081 agaggattta tgagtgaaac gcctgtaacc ggagctcata gggcaaaaac gtttcagtca
    13141 gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt
    13201 ctacaatctg tgcaagatca tgttaccggt aaaataacca taaaggataa gcgctgatat
    13261 tactcaaact cattccagga acgaccatca cgggtaatca tcgccaccga ggcaacgggt
    13321 ccccaggttc cggcctgata cggtttcggc gcatcattgt ccatcgccca cgcctcagta
    13381 atggagtcta cccatttcca ggcttcttcc acttcgtcgc gacgtacaaa cagtgcctga
    13441 ataccacgca tggtttccag cagcaaacgt tcataggcat ccgccagatg cgtctgatta
    13501 aaggtttctg aatagctcag atccagcttg gtgatttgca ggttatgttt gtggtcaagg
    13561 ccaggaactt tattcagtac ctggatatcc acgccttcat caggttgcag acggatagtc
    13621 agtttattct gcggcagatc ctgccacgac tctttaaaca gattcagttc aggtgttttg
    13681 aaatagacca cgacttcaga acatttggtc ggcagacgtt taccagtacg caggtagaat
    13741 ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta
    13801 ttgctgcttt tgttcgcgcc ctcttcttcc agatatcccg gcactttttt gccctgggcg
    13861 aagcccgcag tatattgccc gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg
    13921 cgcagggact tcagtacttt cactttttca tcgcggatgc tgtctgcgct caggtcagat
    13981 ggcggagaca tcgcaatcat gcagagaatt tgtagcaggt ggttctgaat catgtcacgc
    14041 atctgaccgg ctttatcaaa atagccccag cgcccttcga tccccacttc ttctgccacg
    14101 gtaatctcaa catgatcaat ggtgcgattg tcccagttat tcacaaacag ggagttagca
    14161 aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa gatagtggtc gatacggtaa
    14221 acctggcact cctcgaagta ttcgccaacc tgatcattga tttcctgcga ggtcgccagc
    14281 gacgtcccca gcggtttctc catgactacg cgtgccggtt tagcattcag ttttgcctcg
    14341 ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta gttaatagtg
    14401 atacgatttt tttgatccag catcgcgccg agacggttga atgcagcagt gtcattgaca
    14461 tcgagattac aaaaatccag acgtgcactc agggtgtccc ataaaccttc atcaatggtt
    14521 tctttcatga aagtttcgag cgcctcgcgg acaactttgg tatatgccgc tttatcccag
    14581 tcagcacgcc ctacgccgat aatccgggtg tccgggttga gctgaccggc tttttccagt
    14641 tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc
    14701 aggtcacagg cctgggctgt ttgcgttacc gccatgtcat tctccttaag ttaactaacc
    14761 cggtacttaa gccagggtat acttgtaatt ttcttacggt gcactgtact gcttttccga
    14821 gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta cgggaaaagc gcgcaaagtg
    14881 cggcaaaact gataaaaaaa tcatcgtttt ccattcggta aaaatctgac actgctcatg
    14941 ttatgaaaaa aaaataacaa cttttttatc tgcttttgtc attaacacgg cacacaggcg
    15001 taatatctga caaaactgca tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga
    15061 gtctctttac atcatgaata tgctggaaaa aatccagtct cagctggaac atttgagcaa
    15121 atcagagcgc aaagttgccg aggtcattct ggcttcgccc gataacgcga tccattcgag
    15181 tattgctgct ctggcgctgg aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg
    15241 cagcatggac acgcgcggtt ttcctgattt taaacttcat ctggcacaaa gtctggcgaa
    15301 tggcactccc tatgttaatc gcaacgtcaa tgaagatgac agcgttgaat catacacggg
    15361 gaaaatattt gagtccgcaa tggcaacgct cgatcatgtc cgtcattcac tggataaatc
    15421 tgccatcaac cgcgccgtcg acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt
    15481 aggctcttct gccgccgttg cccacgatgc gatgaataag ttctttcgtt ttaatgttcc
    15541 ggtggtgtac tccgatgata tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg
    15601 agacgtggtg gtgctgattt ctcacacagg aagaacaaaa aatctggtcg agctggcgca
    15661 gctggcacgc gaaaacgacg ccatggtgat tgccctcacc tctgcgggta ccccgctcgc
    15721 ccgggaagca acgctggcaa ttaccctcga cgtaccggaa gatactgaca tttatatgcc
    15781 catggtttct cgacttgcac agctgaccgt gatagatgtg ctggcgacag gatttacttt
    15841 gcgacgcggt gcaaaattca gagataactt gaagcgggtc aaggaagcgc tgaaggaatc
    15901 gcgttttgat aagcagttac ttaatttaag tgacgatcgc taaaagcgac tgtcactgtc
    15961 ctaatcttat acgacatccg aatgagatta atttatcgcc atcgcggcgt tatttcattc
    16021 ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac atgtccaaaa
    16081 ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc gataataatc
    16141 ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct cacggctcgc
    16201 ctgaagatca caaaatgcac gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc
    16261 atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc tttaaagaag
    16321 gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg ggtaaaggtg
    16381 aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg
    16441 gtgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa gttcagggca
    16501 tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa ggtatcaaca
    16561 aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca gacattaaga
    16621 ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt ggcgaagatc
    16681 tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg
    16741 ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga tgacatcatc ctcgcctctg
    16801 acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg
    16861 gcattcagaa agcgttgatc cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga
    16921 cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa gtcatggacg
    16981 tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa actgccgctg
    17041 ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa
    17101 tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat gtggaagaag
    17161 ctattgccat gtcggcaatg tacgcagcta accacctgaa gggcgttacg gcgatcatta
    17221 ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct ggtctaccaa
    17281 ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat cgtggcgtta
    17341 cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc
    17401 tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc cagggcgacg
    17461 tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag taagtacgtt
    17521 gccggatgcg gcgaaaacgc cacatccggc ctatagttca atgatagttc aacagatttc
    17581 gaatattctg aagcaaactt gaacttatca tcaggcgaag gcctctcctc gcgagaggct
    17641 tttttatttg atgggataaa gatctttgcg cttatacggc tggatttcgc ccggtttgcg
    17701 agttttcagc aattttagta tccaggtgta ttgttctggt cgcggaccaa caaaaatctc
    17761 gacttcttca ttcatccgcc gctcaatcgt atgatcatcc gcctctaaca gatcatccat
    17821 cggtgggcgc acctgaatcg tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg
    17881 tacaacgcgc gcacggcata ctttcatcaa acgaccaatc gcgggcaacg tcgctttata
    17941 ggtggcaaag aaatccacaa attcgctgtg ctctgggcca tgatcctgat cgggtaaata
    18001 atatccccag tacccctgac gtaccgactg gatgaatggt ttaataccgt catttctcgc
    18061 atgcagacga ccgccaaagc gacgacgcac cgtgttccag acataatcaa aaaccgggtt
    18121 gccctgatta tggaacatcg ctgccatttt ctgcccttgc gaggccatca gcatggcagg
    18181 aatatcgacg gcccaaccgt gcggcaccag aaagataact ttctcgttat tacgccgcat
    18241 ctcttcgatg atctccagcc cttgccagcc aacgcgcggc tgaattttct ccggcccgcg
    18301 tattgccaac tcagccatca ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac
    18361 aatcgcttcg cgttcagctt cactacgttc tggaaagcag agcgacagat tgattaacgc
    18421 acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg
    18481 atcacggaac tttggcggcg ttaaagcgat acccgccatc gctgctacac ccagccatgc
    18541 tccccagtag cgcgggtggc gaaaggattt atcaaactca ggaatgtatt cgctattatt
    18601 ttttttcgtt tccatgcttt tccagtttcg gataaggcaa aaatcaatct ggtgatagtg
    18661 tagcggcgca acttgccccg caccaaataa aaaaagccgg tactgactgc gtaccggctg
    18721 cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg
    18781 aattcgcgac gatcggagcc ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg
    18841 tttacggcct gctggtttat ccatacttca tagtgcagat gcggcccagt tgaacgtccg
    18901 gtattaccgg aaagcgcgat acggtcgcca cgtttcacct tctgtcccgg tttcaccaga
    18961 atcttgcgca agtgcatata acgcgtggtg tagctgcgac catgacgaat agccacataa
    19021 taacctgctg cgccactgcg tttggcaacc accacttcac cgtcacccac tgaaagcact
    19081 ggcgtacctt gtggcatggc gaaatcaaca cctctgtgtg gtgcaacgcg acctgtcacc
    19141 ggattagttc gacgcgggtt aaagttagac gagatacgga actgtttcgc cgtcgggaat
    19201 cgcaagaatc ctttcgccag accagtaccg ttacgatcgt agaatttgcc atcctcagcg
    19261 cggattgcgt aataatcttt accttctgaa cgcaaacgta cgcccagcag ctggctttgc
    19321 tcacgtttac catcaagcat ttctcgagac attaataccg caaattcatc gccttttttc
    19381 agtttgcgga aatccatttg ccactgcatg gctttaatca ctgcgctcac ttcggcgctg
    19441 gttaaaccgg cgtttctggc gctggcaaca aagctccctc cgacggtacc tttcagcaga
    19501 ttgttaaccc actctccttg ctgcatttcg ctggtcattt taaaaccgtt agcggcagta
    19561 cggtcatagg ttcgggtttc acgacgagac acttcccagg tgaggcgctg cagttcgccg
    19621 tccgcggtta atgtccagga gagttgttga ccgattttca ggttacgcaa ttctttgtcg
    19681 gcagcagcga gttgggtgat atcacccata tcaataccat actgattgag aatgctgctt
    19741 agcgtatcgc cagtggaaac aacatattca tgcacgcccg cttcaccggc gattttgtca
    19801 tccagttcgt cctggggaat ggcttcatct tcttgtgcag cttgatcaat cggctcactg
    19861 gcttcaggta agagcgaacg aatttcgttc tgttccagct caatggtttt gacaattggc
    19921 gtggcatcac ggtgataaac atagggccgc cagacagcga cggccagagt aagaacggtg
    19981 agcgacccca acataacgcg gtgtggtcgc ggtaaattat taaacgccag ggcgacagag
    20041 cgggctatct gttgcacgta atcacttcct cattaatctc ctttcaggca gctcgcatac
    20101 tggttggcta attgactcag gaattctgaa tagcttgttt tacccagttt gatattcgtc
    20161 cccaggggat ccaacgttcc catacgaacg gatgtccctc gtgcgacgct ctcaacgacc
    20221 gctggcctga actgtggctc agcaaaaacg caggttgctt tttgctcaac caactgtgtt
    20281 cttatttcat gtaaacgctg cgcgccaggt tgaatctcag ggttaacggt aaaatgacca
    20341 agcggtgtca gtccgaactg tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa
    20401 cctttcccct tgagcggcgc gagctcgtta ccaacctgcg tttcggttga ggccaattgt
    20461 gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac tttgcggcat aagttccact
    20521 aattttccat ggattgcaac cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc
    20581 atgttgaaat cgccgtgatg gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca
    20641 tcatcgccgt gaatactttt catcagtagc ggtttcacat cttcaagctg cgcaatcgtt
    20701 acctgcttcg cttctggtaa tttgcttacc ggtttttgca taaacgcttc catctccggg
    20761 ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta catccgatgg acgcagtgaa
    20821 taatcatgtt ctgaagcgcc gtcaggcagt aaaacctccg tttctgttac accatcagca
    20881 atggcagaag cgatgaaccc aacgggttta agcgaagcga caacggcggc atctgcggcc
    20941 tgtgttgcac ctccccagag agcggcggat aatgctgcga aaagaagcgt ttttttatgt
    21001 aacataatgc gaccaatcat cgtaatgaat atgagaagtg tgatattata acatttcatg
    21061 actactgcaa gactaaaatt aacatgacaa gtctggtttc cctggaaaat gtctcggttt
    21121 cttttggcca acgccgcgtc ctctctgatg tgtcgctgga acttaaacct ggaaaaattt
    21181 tgactttact tgggccaaat ggcgcaggta agtcgacact ggtacgggta gtgctcgggc
    21241 tggtaacacc cgatgaaggg gttatcaaac gcaacggaaa actgcgcatc ggctatgtac
    21301 cgcagaagct gtatctcgac accacgttgc cactgaccgt aaaccgtttt ttacgcttac
    21361 gccctggtac acataaagaa gatattttgc ctgcactgaa acgtgtccag gccgggcacc
    21421 tgattaacgc accgatgcaa aagctctctg gtggcgaaac gcagcgtgta ctattagcgc
    21481 gagcattgtt aaattgcccg caattattag tgctggatga acccactcaa ggcgtggatg
    21541 taaatggcca agtggcgtta tatgacctta ttgaccagtt gcgtcgtgaa ctggattgtg
    21601 gcgttttaat ggtttctcac gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt
    21661 gcctgaatca ccacatttgt tgttccggca caccggaagt tgtttccctg catccggagt
    21721 ttatttcaat gtttggtcct cgtggtgctg aacaactggg tatctatcgc catcatcata
    21781 atcatcgtca cgatttacag ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat
    21841 tgaattatta tttcccggtt ggttagccgg gatcatgctc gcctgtgccg cgggtccgct
    21901 gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc
    21961 attacttggt gtcgcgtttg gtttgttgct ggacgtgaat ccattctatg cggtgattgc
    22021 cgttacgctg ctgctggcgg gcggtctggt atggctggag aagcgtccac agctggcgat
    22081 cgacacgtta ttagggatta tggcgcacag tgccctgtcg ctgggcctag tggtcgttag
    22141 tctgatgtct aacattcgtg ttgatttgat ggcttacctg ttcggtgatt tactggcagt
    22201 gacgccagaa gatctcatct ctattgcgat tggcgtggtc atcgtggtgg ctattttgtt
    22261 ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg gatctggcgt ttgttgatgg
    22321 tgtgaaatta cagcaagtga aattgttgtt gatgctggtg acggcattga cgattggtgt
    22381 agcgatgaaa tttgtcggtg cgttgattat tacttcgttg ctgattattc ctgcagctac
    22441 tgcgcgtcgc tttgcccgta cgccggaaca gatggctggt gtcgctgttt tggtggggat
    22501 ggtggcagta actggcggtt taaccttttc cgcgttttac gatacgccgg cgggtccgtc
    22561 ggtggtgcta tgtgcggcac tgttatttat tatcagtatg atgaaaaagc aggccagcta
    22621 atctatcgct gaacacattt gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg
    22681 ctatctctgg ctggcctgat aagacgcgaa cagtgtcgca tcagacacac tgccagtgtc
    22741 ggatgcggcg cgagcgacca atccgactta cggcatttct ggcggcgtta tgccaaagtg
    22801 attccacgcc cgcgtcgtcg ccatacgccc gcgcggtgta cgctgcaaaa agccttgctg
    22861 aatcaaataa ggttccagta catcttcaat ggtttcacgt tcttcgccaa tggctgccgc
    22921 caggttatcc agacctaccg gcccaccaaa gaacttatcg attaccgcca gcaacaattt
    22981 gcggtccata taatcgaaac cttcagcatc gacattcaac atatccagcg cctgagcagc
    23041 gatatctgcc gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag
    23101 cagacggttg gcaatacgtg gcgtaccgcg agcgcgacga gctacttcct gcgcaccgtc
    23161 atcactcatc tcaagcccca taaagcgtgc actgcgactg acgatatatt gcagatccgg
    23221 cacctgataa aactccagac gttgcacaat accaaaacgg tcgcgcaacg gtgatgtcag
    23281 cgaacctgcg cgcgtggttg cgccaatcag ggtaaacggc ggcaaatcaa ttttaatgga
    23341 gcgtgccgcc ggaccttcac caatcatgat atccagttgg tagtcttcca ttgccgggta
    23401 cagcacttct tcaacaactg gcgataaacg gtggatctca tcaataaaca gcacgtcatg
    23461 cggttcaagg ttagtgagca tcgcagccaa atcgcccgcc ttttccagca ccggaccaga
    23521 agtcgtgcgt aaattaacgc ccatttcatt ggcgacaatg ttggcaagcg tagttttacc
    23581 caaccccgga ggaccaaaaa tcaacaaatg atcgagggca tcgccgcgca gtttcgctgc
    23641 tttgatgaaa atctccatct gtgaacgaac ctgcggctga ccaacatact cttccagtaa
    23701 tttagggcga atggcgcgat ctgctacatc ttccggcaaa gtggtaccgg cagaaatcag
    23761 acggtctgct tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat
    23821 gtttcactgc tggcgtcagg gcgagcgatt ttgctcacca tgcggcttgc ttcttgtggt
    23881 ttatagccta gcgccaccag cgcggcaact gcttcttgtt cagcatcgtc ggtcgccggg
    23941 ctggcaggag acgtgagtac caggtcggcg gctggcgtaa agagatcgcc atgcaaacct
    24001 ttaaatcggt ctttcatttc aacaatcaag cgttcggcgg tttttttgcc aatacccggc
    24061 agtttcacca gtgcccccac ttcttcacgc tcaacggcat taacgaactg ctgcgctgac
    24121 attccggaga ggatcgccag ggccaacttc gggccgacgc cgttggtttt gatcaactct
    24181 ttgaacaacg tgcgttcttg tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc
    24241 accacaaagt gggtgaaaac gatcgcttcc tgacccgctt cagggagttc ataaaaacag
    24301 gtcatcggca tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt
    24361 tgtttttcaa tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat
    24421 caaagataat gctgtatgat aaaaaaatgc tggatagata tccagcgtag gatgaagaaa
    24481 acttgcgagg tgtcccgatg atctgaaaaa tggcacagta taatttattc aacaggttat
    24541 attggaagca aatatttgaa tattacatat tcagtgaaga aatgcgtaat aaaaatatac
    24601 attgcgactc ctgaaaaaat aattttttta tgctattacg tatattcgta tctatttcaa
    24661 tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga tatcgtcttt
    24721 acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggagtaat
    24781 cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt
    24841 cccctttcaa ccatcaccac
//
LOCUS       DADWTH010000054        24461 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.054, whole genome shotgun sequence.
ACCESSION   DADWTH010000054 DADWTH010000000
VERSION     DADWTH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.054"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..488)
                     /locus_tag="JNY02_002350"
     CDS             complement(<1..488)
                     /locus_tag="JNY02_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBB9433870.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKMKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            761..949
                     /locus_tag="JNY02_002351"
     CDS             761..949
                     /locus_tag="JNY02_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBB9433871.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            961..1671
                     /gene="bcsQ"
                     /locus_tag="JNY02_002352"
     CDS             961..1671
                     /gene="bcsQ"
                     /locus_tag="JNY02_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBB9433872.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1668..4281
                     /gene="bcsA"
                     /locus_tag="JNY02_002353"
                     /pseudo
     CDS             1668..4281
                     /gene="bcsA"
                     /locus_tag="JNY02_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4292..6631
                     /gene="bcsB"
                     /locus_tag="JNY02_002354"
     CDS             4292..6631
                     /gene="bcsB"
                     /locus_tag="JNY02_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBB9433873.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCEKPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNLPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6638..7744
                     /gene="bcsZ"
                     /locus_tag="JNY02_002355"
     CDS             6638..7744
                     /gene="bcsZ"
                     /locus_tag="JNY02_002355"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBB9433874.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7803..7979
                     /locus_tag="JNY02_002356"
                     /pseudo
     CDS             7803..7979
                     /locus_tag="JNY02_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8307..10055
                     /gene="bcsC"
                     /locus_tag="JNY02_002357"
                     /pseudo
     CDS             8307..10055
                     /gene="bcsC"
                     /locus_tag="JNY02_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10137..12125
                     /gene="hmsP"
                     /locus_tag="JNY02_002358"
     CDS             10137..12125
                     /gene="hmsP"
                     /locus_tag="JNY02_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBB9433875.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12309..13595
                     /gene="dctA"
                     /locus_tag="JNY02_002359"
     CDS             12309..13595
                     /gene="dctA"
                     /locus_tag="JNY02_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBB9433876.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13816..15312
                     /locus_tag="JNY02_002360"
     CDS             13816..15312
                     /locus_tag="JNY02_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBB9433877.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15408..16337)
                     /gene="kdgK"
                     /locus_tag="JNY02_002361"
     CDS             complement(15408..16337)
                     /gene="kdgK"
                     /locus_tag="JNY02_002361"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBB9433878.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGAGSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSVENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16569..17336
                     /gene="pdeH"
                     /locus_tag="JNY02_002362"
     CDS             16569..17336
                     /gene="pdeH"
                     /locus_tag="JNY02_002362"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBB9433879.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17406..19466
                     /locus_tag="JNY02_002363"
     CDS             17406..19466
                     /locus_tag="JNY02_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBB9433880.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19648..20970)
                     /locus_tag="JNY02_002364"
     CDS             complement(19648..20970)
                     /locus_tag="JNY02_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBB9433881.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21381..22394)
                     /gene="yhjD"
                     /locus_tag="JNY02_002365"
     CDS             complement(21381..22394)
                     /gene="yhjD"
                     /locus_tag="JNY02_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBB9433882.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22443..23342)
                     /locus_tag="JNY02_002366"
     CDS             complement(22443..23342)
                     /locus_tag="JNY02_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9433883.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23862..>24461
                     /locus_tag="JNY02_002367"
     CDS             23862..>24461
                     /locus_tag="JNY02_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB9433884.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5792 a         6165 c         6729 g         5775 t
ORIGIN      
        1 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
       61 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
      121 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
      181 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
      241 aataatttca ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
      301 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
      361 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
      421 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
      481 tccctcatgt caccattttg tctgattatc agtttactag cgaaagctaa gaaataaacc
      541 taccattgaa attaaaactt atcagattta gcatgtgaat caatttagaa ggtcacaaga
      601 cgtgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa agtcaaaaag
      661 tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg gttagaattg
      721 tatcatgaat ggtacggtta tttcataggg atcaagcaaa atgaataaca atgaaccaga
      781 tactctgcct gatcccgcga taggctatat cttccagaat gatattgtgg cgttaaagca
      841 ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac agttggccgc
      901 ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg ggattggtga
      961 atggccgtac tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac catcaccgcc
     1021 gcattagcct ggtcattaca aatgttggga gaaaatgtcc tagtggtcga tgcctgcccg
     1081 gacaacttgt tgcgcctgtc atttaatgtt gattttaccc accgtcaggg ctgggccaga
     1141 gcgatgctgg atggccagga ctggcgtgac gctgggttgc gctacacctc gcagctcgat
     1201 ttgctgcctt ttggtcagtt atccattgaa gaacaagaaa atccacagca ctggcaaacc
     1261 cggctgagcg atatttgctc cggcttacag caactaaaag ccagcgggcg ttaccagtgg
     1321 attttaatcg acttaccgcg tgatgcctcg cagataaccc accagctgct gagtttgtgc
     1381 gagcactcgc tggcaatcgt caatgtggat gccaactgcc atatccgact gcatcagcaa
     1441 gcgctgccgg atggcgcaca tattttgatt aatgacttcc gtattggcag tcaggttcag
     1501 gacgatattt accagctttg gttgcaaagc cagcgccgat tactgccgat gctcattcat
     1561 cgtgatgaag cgatggctga atgcctggcg gctgaagaga tactgacgct ggcgaactgg
     1621 tgcctgttga actactccgg gctgaaaacg ccagtcggga gtgcatcatg agtatcctga
     1681 cccggtggtt gcttatcccg ccggtcaacg cgcggcttat cgggcgttat cgcgattatc
     1741 gtcgtcacgg tgcgtcggct ttcagcgcga cgctcggctg tttctggatg atcctggcct
     1801 ggatttttat tccgctggag cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc
     1861 tgtatacgcc aacgcctcgc gtccgcgtcc gctggacccg gtccgttatc tcattcaaac
     1921 atgctggtta ttgatcggtg catcgcgcaa agaaacgccg aaaccgcgca gcgggcattt
     1981 tcaggtctgc aaaatattcg tggacgttac catcaatgga tgaacgagct gcctgagcgc
     2041 gttagccata aaacacagca tctggatgaa aaaaaagagc tcggtcattt gagtgccggg
     2101 gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc tgattctggc gttaatctgc
     2161 gttactcagc cgtttaaccc gctggcgcag tttatcttcc tgatgctgct gtggggggta
     2221 gcgctgatcg tacggcggat gccggggcgc ttctcggcgc taatgttgat tgtgctgtcg
     2281 ctgaccgttt cttgccgtta tatctggtgg cgttacacct ctacgctgaa ctgggacgat
     2341 ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg aaacgtacgc gtggattgtg
     2401 ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc gtcagccggt gccattgccg
     2461 aaggatatgt cgctgtggcc gtcagtggat atctttgtcc cgacttacaa cgaagatctc
     2521 aatgtggtga aaaataccat ttacgcctcg ctgggtatcg actggccgaa agacaaactg
     2581 aacatctgga tcctcgatga cggcggcagg gaagagtttc gccagtttgc gcaaaacgtg
     2641 ggggtgaaat atctcgcccg caccactcat gaacatgcga aagcgggcaa catcaacaat
     2701 gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg actgcgacca cgtaccaacg
     2761 cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag agaaacagct ggcgatgatg
     2821 cagactccac accacttctt ctcgccggac ccgtttgaac gcaacctggg acgttttcgt
     2881 aaaacgccga acgaaggcac gctgttctat ggtctggtgc aggatggcaa cgatatgtgg
     2941 gacgccactt tcttctgcgg ttcctgtgcg gtgattcgtc gtaagccgct ggatgaaatt
     3001 ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata cttctctgcg tttgcaccgt
     3061 cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg cggggctggc gaccgaaagt
     3121 ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg ggatggtaca aatcttccgt
     3181 ctcgataacc cgctcaccgg taaaggactg aagtttgctc agcggctgtg ctacgtcaac
     3241 gccatgttcc acttcttgtc gggcattcca cggctgatct tcctgactgc gccgctggcg
     3301 ttcctgctgc ttcatgccta catcatctat gcgccagcgt tgatgatcgc cctattcgtg
     3361 ctgccgcata tgatccatgc cagcctgacc aactcgaaga tccagggcaa atatcgccac
     3421 tctttctgga gtgaaatcta cgaaacggtg ctggcgtggt atatcgcacc accgacgctg
     3481 gtggcgctga ttaacccgca caaaggcaaa tttaacgtca ccgccaaagg tggactggtg
     3541 gaagaagagt acgtcgactg ggtgatctcg cggccctaca tcttccttgt tctactcaac
     3601 ctggtgggcg ttgcggtagg catctggcgc tacttctatg gcccgccaac cgagatgctc
     3661 accgtggtcg tcagtatggt gtgggtgttc tacaacctga ttgttcttag cggcgcagtt
     3721 gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc gcgtggagat gacgatgccc
     3781 gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta ccgttcagga tttctccgac
     3841 ggtggtttgg ggatcaagat caacggtcag gcgcagattc tggaagggca gaaagtgaat
     3901 ctgttgctta aacgcggtca gcaggaatac gtcttcccga cccaggtggc gcgcgtgatg
     3961 ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc agcaacatat cgattttgtg
     4021 cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc aggacagcta cccggaagat
     4081 aagccgctgg aaagtctgct ggatattctg aagctcggct tccgtggcta ccgccatctg
     4141 gcggagtttg cgccttcttc ggtgaagggc atattccgtg tgctgacttc tctggtttcc
     4201 tgggttgtat cgtttattcc gcgccgcccg gagcggagcg aaacggcaca accatcggat
     4261 caggctttgg ctcaacaatg atgataacgc gatgaaaaga aaactattct ggatttgtgc
     4321 agtggctatg gggatgagtg cgttcccctc tttcatgacg caggcgacgc cagcaacgca
     4381 accactgatc aatgctgagc cagctgtagc cgcccagacg gaacaaaatc cgcaggtggg
     4441 gcaagtgatg tcaggcgagc agggagctga tgcccccatc gtggcgcaga acggtccttc
     4501 gcgtgatgtg aagctgacct ttgcgcaaat cgcgccgccg ccgggcagca tggtgctacg
     4561 tggtattaac ccgaacggca gcattgagtt tggtatgcgc agcgatgaag tggtgacgaa
     4621 ggcgatgctc aacctcgaat acacgccatc gccatcgtta ctgcctgtcc agtcgcagtt
     4681 aaaggtttat ctcaatgatg agctgatggg cgtgctgcca gtgaccaaag aacagttggg
     4741 taaaaaaacg ctggcgcaaa tgcccattaa tccactgttt attaccgact tcaaccgtgt
     4801 gcggctggag tttgtcggcc attatcagga cgtgtgcgaa aaaccggcca gcaccacgct
     4861 ttggctggat gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa
     4921 gaatgacctg tcacacttcc cggtgccatt ctttgacccg cgcgataacc gtactaacac
     4981 cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg caacaagcat ctgccattgt
     5041 cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag aacctcccgg tgctctataa
     5101 ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac gacaaacggc cggacttcct
     5161 gcgcgatcat ccggcggtaa aagccccggt gattgagatg attaaccatc cgcagaatcc
     5221 ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa gacctgttgc aggcagcgaa
     5281 aggtatcgct cagggtaaca ttctgttccg tggtgaaagc gtggtagtga atgaagtgaa
     5341 accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg gtacgtaccg atcgtccggt
     5401 cacatttggc gaactgaaaa cctatgaaga acagttacaa tccagcggcc ttgagccagc
     5461 agcgattaac gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat
     5521 tgatatggat attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga
     5581 tatcagcctg aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa
     5641 ccgtctgctg ctgcgtattc cggtattaca aggtttgctg gatggcaaaa cagatgtctc
     5701 tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc ttcgactttg agtatatgaa
     5761 cccgatgcca ggcggttcgg tagataactg tattaccttc cagccggtgc agaatcatgt
     5821 ggtgattggt gacgattcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc
     5881 ggatctacgc gcctttgcta acgcgggctt cccattcagc cggatggcgg atctgtcgca
     5941 aaccatcacc gtgatgccga aagcgcctaa cgaagcacag atggaaacgt tgctgaatac
     6001 tgttggtttt atcggcgcac agacgggctt cccggcgatt aatctgacgg tgaccgatga
     6061 tggcagcacc attcagggca aagatgccga catcatgatc atcggtggta tcccggacaa
     6121 actgaaagac gataagcaga tcgacctatt ggtgcaggcg accgaaagct gggtgaaaac
     6181 accgatgcgc cagaccccgt tccccggcat tgtgccggac gagagcgatc gcgcggcaga
     6241 aacccagtca acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc
     6301 gtataacgac cagcgcagcg tgattgcgct gttggcagat agcccacgcg gttatgaaat
     6361 gcttaacgat gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt
     6421 gatccgcgag tacggtatca acagcctacg tgttggcgac gtttattacg taggtcatct
     6481 gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct
     6541 ggcggctatc agtgtgatat tgctggcatg ggtactgtgg cgtctgctgc gaattattag
     6601 tcgtcgtcgt cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggaat
     6661 cgtgacgatg ctgttgttgg ctgcctttag tgttcaggca gcctgtacct ggcctgcctg
     6721 ggagcagttt aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc
     6781 gcgcaaaatc accacctccg aagggcaaag ttacggtatg ttctttgccc tggcggctaa
     6841 cgaccgtgca gctttcgata atattctcga ctggacgcag aacaatctcg ctcagggttc
     6901 tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt
     6961 gctggacagc aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc
     7021 ggggcgtttg tggaaagagc agcgttatac cgacatcggc agcgcgttgc taaaacgtat
     7081 cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc atgttgttac cgggcaaagt
     7141 gggttttgct gaggataaca gctggcgttt taaccccagc tacatgccgc cgacgctggc
     7201 gcagtatttc acccgctttg gcgcgccgtg gaccacgctg cgcgaaacca atcaacattt
     7261 attgctggaa accgccccga aaggcttttc gccagactgg gtgcgctatg agaaagacaa
     7321 aggctggcag ctaaaagccg aaaaaacatt gatcagcagc tacgacgcta tccgcgttta
     7381 catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa gcgcggatgc tcaaccggtt
     7441 taaaccgatg gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc
     7501 tacggggaaa gcgcagggta aaggaccggt cggtttttct gccgccatgc tgcctttttt
     7561 acaaaaccgc gatgcgcagg cagttcagcg ccagcgcgtg gccgataact ttcccggcag
     7621 cgatacctat tacaactatg tgctgaccct gtttggacaa ggctgggatc aacaccgttt
     7681 ccgcttctcg acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca
     7741 ctaaacatat tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca
     7801 accatcagca gaccctgcgg atggacagcg gcaacactaa tgccgtgcgc gggctggcga
     7861 atatttaccg ccagcagtcg ccagaaaaag ctgaagcgtt tatcgcctcg ctctctgcca
     7921 gccagcggcg cagcattgat gatatcgaac gcagcctgca aaacgaccgt ctggcacagc
     7981 aggcagaggt actggaaaac cagggcaaat gggcgcaggc ggcagcactt cagcggcaac
     8041 gactggcgct ggaccccggc agcgtatgga ttacttaccg actttcgcag gatctctggc
     8101 aggccggaca acgcagccag gccgatacgt taatgcgcaa tctggcgcag cagaagccga
     8161 acaacccgga gcaggtttac gcttacgggc tgtacctctc aggtcacaac caggacagag
     8221 cggcgctggc gcatatcaac agcctgccgc gcgcgcagta gaacagcaat attcaggagc
     8281 tggttaatcg actgcaaagc gatcaggtgc tggaaaccgc taatcgcctg cgagaaagcg
     8341 gcaaagaggc tgaagcggaa gcgatgctgc gtcagcaacc accttccacg cgtattgacc
     8401 tcacgctggc tgactgggcg taacaacgac gtgattacac cgccgcccgc actgcatatc
     8461 agaatgtcct gacgcgggag ccaactaacg ccgatgccat tcttggtctg acggaagtgg
     8521 atattgctgc cggtgacaaa gcggcggcac gtagccagct ggcgaaactg cctgccaccg
     8581 ataacacctc gctgaacact cagcggcgcg tggcgctggc acaggcgcag cttggagata
     8641 ccgcagcggc gcagcagacg tttaataagt tgatcccgca ggcaaaatct cagccaccgt
     8701 cgatggaaag cgcgatggtg ctgcgcgacg gtgcgaagtt tgaagcgcag gcgggcgatc
     8761 caaagcaggc gctggaaacc tacaaagacg ccatggtcgc atccggtgtg actacgacgc
     8821 gtccgcagga taacgacacc tttacccgac tgacccataa cgacgagaaa gatgactggc
     8881 tgaaacgcgg cgtgcgcagc gatgcggcgg acctctatcg ccagcaggat cttaacgtca
     8941 cccttgagca cgattactgg ggttcgagcg gcaccggtgg ttactccgat ctgaaagcgc
     9001 acactaccat gttgcaggtg gatgcgccgt attctgacgg gcggatgttc tttcgcagtg
     9061 atttcgtcaa tatgaacgtc ggcagtttct ccactaatgc cgatggcaaa tgggatgaca
     9121 actggggcac ctgtacatta caggactgta gcggcaaccg cagccagtcg gattccggtg
     9181 ccagcgtggc ggtcggctgg cgaaatgacg tttggagctg ggatatcggt accacgccga
     9241 tgggcttcaa cgtggtggat gtggtcggcg gcatcagtta cagcgatgat atcgggccgc
     9301 tgggttacac cgttaacgcc caccgtcggc ccatctccag ttctttgctg gcctttggtg
     9361 ggcaaaaaga ctccccgagc aataccggga aaaaatgggg tggcgtacgt gccgacggtg
     9421 tggggctaag tctgagctac gataaaggtg aagtaaacgg cgtctgggca tcgcttagtg
     9481 gcgaccagtt aaccggtaaa aatgtcgaag ataactggcg cgtgcgctgg atgacgggct
     9541 attactataa ggtcattaac cagaacaatc gccgcgtcac aatcggcctg aacaacatga
     9601 tctggcatta cgacaaagat ctgagtggct actcactcgg tcagggcggt tactacagtc
     9661 cgcaggaata cctgtcgttt gccataccgg tgatgtggcg ggagcgcacg gaaaactggt
     9721 cgtgggagct gggggcgtct ggctcgtggt cgcattcacg caccaaaacc atgccgcgtt
     9781 atccgctgat gaatctgatc ccgaccgact ggcaggaaga agctgcgcgg caatccaacg
     9841 atggcggcag cagtcagggc ttcggctaca cggcgcgggc attacttgaa cgacgtgtta
     9901 cttccaactg gtttgttggc acggcaattg atatccagca ggcgaaagat tacgcaccca
     9961 gccatttcct gctctacgta cgttattccg ccgccggatg gcagggtgac atggatttac
    10021 cgccgcagcc gctgatacct tacgccgact ggtaagtttt cagatagcgc ctctcttaat
    10081 gccgctgcga tcgggtatac tcgggcggca atctgggatt tccgggggga gacaatttgc
    10141 gcgtaagtcg ctcgttaaca atcaagcaga tggcaatggt ggcagccgtt gtcctggtgt
    10201 tcgtttttat tttttgcacc gttttgctgt tccatctggt ccagcagaat cgctataaca
    10261 cggctacgca actggaaagc attgctcgct ctgtccgcga acccttatct tcagctattt
    10321 tgaaaggcga tattcccgaa gcggaagcta ttcttgccag cattaaaccg gcaggcgtgg
    10381 tcagccgtgc cgatgtagtg ctgcctaacc agttccaggc gctgcgtaaa agttttattc
    10441 cagagcgtcc ggtgccggta atggttactc gcctgtttga gctaccggtt caaatctcgc
    10501 tgggcgtcta ctcgctcgaa cgtccggcaa atccgcagcc aatagcctat ctggtgctac
    10561 aggcggattc cttccgtatg tataagttcg tgatgagcac tctctcaacg ttagtgacca
    10621 tttacttact tttgtcgctt atcctgaccg tcgccatcag ctggtgcatt aaccgcctga
    10681 ttttgcatcc gttacgcaat attgctcgcg aacttaacgc catcccagcc caggagcttg
    10741 ttggtcacca actggcatta ccgcgtctgc atcaggacga tgaaatcggt atgttggtgc
    10801 gcagttacaa cctcaaccag caattgctgc agcgccatta tgaagaacag aacgaaaatg
    10861 cgatgcgctt cccggtgtcg gatttgccga acaaagcctt gctgatggag atgctggagc
    10921 aggttgtcgc gcgtaaacaa accaccgcgc tgatgatcat cacctgtgaa accctgcgtg
    10981 atactgcggg cgtgctgaaa gaggcgcaac gagaaattct gctgctgacg ctggtggaaa
    11041 aactcaaatc ggtactgtcg ccacgtatga tcctcgcgca gattagcggt tatgactttg
    11101 ctgtcattgc caacggtgta caggaaccgt ggcacgcaat cactttgggt cagcaagtac
    11161 tcactatcat gagcgagcgc ctgccgattg aacgtattca actccgtccg cactgtagca
    11221 ttggcgtggc gatgttctac ggcgatctca ccgccgaaca gctttacagt cgcgctattt
    11281 ctgcggcatt taccgctcgc cataaaggca agaatcagat tcagttcttt gatccgcagc
    11341 agatggaagc agcccagaag cggttgacgg aagagagcga tatccttaat gcactggaaa
    11401 atcatcagtt tgcaatttgg ttacagccac aggtcgagat gaccagcggt aaactggtca
    11461 gtgcggaagt gttactgcgt atccagcaac cggatggcag ttgggacctg ccggatggct
    11521 taatcgatcg cattgagtgc tgtgggctga tggttaccgt cggtcactgg gtgctggaag
    11581 agtcctgtcg attgcttgca gcctggcaag agcgcggcat tatgctgccc ttgtcggtaa
    11641 acctctctgc gctgcaactg atgcacccga atatggtggc ggatatgctg gaactgttaa
    11701 cccgctatcg cattcagccg ggaacactga ttctggaagt gacagaaagc cgacgtattg
    11761 acgaccctca tgctgcggtg gcaatcctcc gtccgctgcg caatgccgga gttcgggtgg
    11821 cgctggatga tttcggtatg ggctacgcag ggctgcgtca gctgcagcat atgaaatcgt
    11881 tgccaatcga cgtactgaaa atcgacaaaa tgtttgttga aggcttgccg gaagatagca
    11941 gcatgattgc tgcaattatc atgctggcgc agagcctgaa cttacaaatg attgccgaag
    12001 gcgtggagac tgaagcacaa cgagactggc tggcaaaagc gggcgttggt attgcccagg
    12061 gcttcctttt tgctcgccca ctccctattg aaatcttcga agagagttac ctggaagaaa
    12121 agtagctacc ccaaactgat tacaaaactt taaaaagtgc tggtttgtgc gagccagctc
    12181 aaacttttta acctttttgt ttcaattatg atccaggtac atttctgtga tgttgtctgg
    12241 gtgttatttt aaggccgcag gtacccccat aaccttacaa gacctgtggt tttactaaag
    12301 gacaccctat gaaaacctct ctgtttaaaa gcctttactt tcaggtcctg acagcgatag
    12361 ccattggtat tctccttggc catttctatc ctgaaatagg cgagcaaatg aaaccgcttg
    12421 gcgacggctt cgttaagctc attaagatga tcatcgctcc tgtcatcttt tgtaccgtcg
    12481 taacgggcat tgcgggcatg gaaagcatga aggcggtcgg tcgtaccggc gcagtcgcac
    12541 tgctttactt tgaaattgtc agtaccatcg cgctgattat tggtcttatc atcgttaacg
    12601 tcgtgcagcc tggtgccgga atgaacgtcg atccggcaac gcttgatgcg aaagcggtag
    12661 cggtttacgc cgatcaggcg aaagaccagg gcattgtcgc cttcattatg gatgtcatcc
    12721 cggcgagcgt cattggcgca tttgccagcg gtaacattct gcaggtgctg ctgtttgccg
    12781 tactgtttgg ttttgcgctc caccgtctgg gcagcaaagg ccaactgatt tttaacgtga
    12841 tcgaaagttt ctcgcaggtc atcttcggca tcatcaatat gatcatgcgt ctggcaccta
    12901 ttggtgcgtt cggggcaatg gcgtttacca tcggtaaata cggcgtcggc acactggtgc
    12961 aactggggca gctgattatc tgtttctaca ttacctgtat cctgtttgtg gtgctggtat
    13021 tgggttcaat cgctaaagcg actggtttca gtatcttcaa atttatccgc tacatccgtg
    13081 aagaactgct gattgtactg gggacttcat cttccgagtc ggcgctgccg cgtatgctcg
    13141 acaagatgga gaaactcggc tgccgtaaat cggtggtggg gctggtcatc ccgacaggct
    13201 actcgtttaa ccttgatggc acatcgatat acctgacaat ggcggcggtg tttatcgccc
    13261 aggccactaa cagtcagatg gatatcgtcc accaaatcac gctgttaatc gtgttgctgc
    13321 tttcttctaa aggggcggca ggggtaacgg gtagtggctt tatcgtgctg gcggcgacgc
    13381 tttctgcggt gggccatttg ccggtagcgg gtctggcgct gatcctcggt atcgaccgct
    13441 ttatgtcaga agctcgtgcg ctaactaacc tggtcggtaa cggcgtagcg accattgtcg
    13501 ttgctaagtg ggtgaaagaa ctggaccaca aaaaactgga cgatgtgctg aataatcgtg
    13561 cgccggatgg caaaacgcac gaattatcct cttaatccca ccacttatgc ccgcactccc
    13621 ttccccgagt gcgggtgttt gcgcataatt gacccctgtg cctgaactta ccacctgggt
    13681 gagttcaggt ttatttcact tcttccgtga cattttcatg ttcttgcggt ctaacacgaa
    13741 gtgtttttaa cgtcatttag actgaccgac aacggacagt ttttttatta ccaggatagt
    13801 tgatcagggg ttcacatgca gggcataaaa attcgacttt tagcgggcgg tttgctgatg
    13861 atggccactg ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag
    13921 gggacgcttt ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat
    13981 cgtgttgaaa ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc
    14041 ggttacagtc acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca
    14101 caggcgcgtt cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt
    14161 gtctcttatg acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg
    14221 aaagaagcgc tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc
    14281 atcaaccacg cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag
    14341 ggctggtggc gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg
    14401 ctgaaacaac ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg
    14461 gatgcaatga cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc
    14521 aacaaaacgt ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt
    14581 tctccgctgc gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta
    14641 tctatcatgt gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac
    14701 tggcgtgcgg acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc
    14761 agtgacagca aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag
    14821 tgtgccatca acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg
    14881 cgtgaactgg cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg
    14941 gcgcaaaaga aactggagct gcagaaactg tttgccgcct atgcacgggc tgataccgat
    15001 attctgatgg gccagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag
    15061 cagtatcaga aactgcggca ggatttcctt aacagcctga ctgtggagat gttaaatcag
    15121 gatctgcgtc agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag
    15181 ccggaattta acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatctacc
    15241 gccgctgcga ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca
    15301 cctgcacagt aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag
    15361 ctggatagag gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc
    15421 gcctcacgcg ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg
    15481 cgtttcgccg cgttttccac actgccgccc gtcaaacgta ccgccagata accggcgctg
    15541 aaagagtcgc cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga
    15601 acatcaacta acgcttcacc agcaatggac accaggcaag aacccgcccc gcgtttcacc
    15661 accacttctt tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga
    15721 ccccacagcg cgtcttcgtc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt
    15781 tgctggtaca cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg
    15841 tcgaaaatca cttttccgcc tttggcgcgg cattcgcgca gcagggaaag cagcttttcg
    15901 cggctggtcg ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc
    15961 gccagctctt cgcaaatcgc cgcagactgc tcgctcgcca gccagaattt ggcggcggct
    16021 tcgttacgcc agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat gtagtaaagg
    16081 cccggaagac ggttttccat ccgttgggtc agggaagtat caacgttctc gccgtgccag
    16141 gcgtccagca tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt
    16201 aatgccgcag gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg
    16261 aaaccgcgct taacgtccgc gtctttctcg gaaagctcaa tcatgcattc gccaatcacg
    16321 gcaatctttt tagacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct
    16381 gtggtggttt gctggtcaac catattaaaa cagtgttcca ttattttttt gagccagaac
    16441 gtattcctga aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt
    16501 ctgcccttac gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga
    16561 catctttgat gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga
    16621 gcttgcagga acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct
    16681 atcaaacatg cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga
    16741 acccttcgca acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga
    16801 tggaggttgt gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc
    16861 acggcctgct ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac
    16921 caaaaatcct gcgccagatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata
    16981 tccgtctgcc gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg
    17041 atgattttgg taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca
    17101 tcaaaatcgc gcgagaactg tttgtgatgc tgcgtcagtc gccggaaggc cgcacactct
    17161 tttctcagct tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag
    17221 aaacaccgga agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt
    17281 ttctttcacg cccggcaccg atggaaacgc tgaatacggc ggttctggcg ctataagctg
    17341 cctcattttc cgccgggctg gactatcttt aggactggca caggaagaat atgaggaagc
    17401 gaacgatgag caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga
    17461 ttgtcgtggc gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca
    17521 accagaaagt ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg
    17581 tgtgggagcg gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac
    17641 atgcagaaga catcattctt ggtaatccac cggatattcc cgaagtcacg atggtgcatt
    17701 tgccgcgagt agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc
    17761 cgtggatcaa gctcgaaaag cccgacgcgc gcctgattcg cctctctgaa aagaacaata
    17821 actggacgtt taatcttgcc aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat
    17881 ggtcgtttcg gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag
    17941 taagcaaagc ggatctggaa atttttgttg atccgttagg caagccgctg ccattcagcg
    18001 aagttactgg atcgaaaggt aaagcggata aagaaaaggt gggcgattac atttttggcc
    18061 tgaaggcgca gggacgttat aacggtgaac cgctcacggg tacgggaaaa ataggcggta
    18121 tgctggcgct gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc cgttcaggta
    18181 atacccgtgt tgcttttgat ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt
    18241 tacggcttaa attttctggc gattcactgg gtgatctcta tgaactgacg ggcgttctgc
    18301 tgcccgatac cccgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa
    18361 aatcgtcggt ctttgattat cgcggtttta atgggcgaat tggcgatagc gatatccacg
    18421 gttctctggt ctacaccacc ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc
    18481 ggcaattgcg gctggcggac ctgggaccgt tgattggcgt tgattccggg aaaggggcag
    18541 aaaagtcgaa acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag
    18601 tgctgcctta tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct
    18661 tcaaagggcg gcgcattgag catggcagta gcctgccgat tagcgatctt tctactcata
    18721 tcatcctcaa aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca
    18781 gcattgcggc gaatattcat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata
    18841 ttcaggctcg tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg atgcagaaga
    18901 cgctggggga aatgaacggt gacgcggaac tacgcggtag cggtaactcg gtggcggcgc
    18961 ttttaggcaa cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca
    19021 acctgatgga gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg
    19081 acgatgaggt gcgggtgaac tgcgcggcgg cgaatctgca tattgccaac ggcgtggcgc
    19141 gcccgcagat ttttgctttc gatactgaga acgcgttgat taatgttacc ggcacggcaa
    19201 gttttgcttc ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta
    19261 tcacactgcg ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga
    19321 aagccggacc gctgattgcc cgtggtgctg ttgctgcggc actggcaacg ctggtaacac
    19381 cggcggcggc gttactggca ctgatctcac cttccgaagg ggaggctaat cagtgtcgga
    19441 cgatattgtc gcagatgaag aagtgatgat aattgccgga tgtctgacat ccggcaatta
    19501 aacagattta gatttttggc aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct
    19561 tatccggcct acaaatcctt gtgaattcaa tatattgtat ggaaattcag tcctgataag
    19621 cgtagcgcat caggcttttc acacttatta caacgactga tgtcgcgtct catgggtcag
    19681 cagcagggcg attaacgtca agccagccat cgccgccaga tataacccca ccgcacctaa
    19741 cccgtagtta gcctgcaacc aagctgcgat atacggcgca acggaagccc cgagaatcga
    19801 cgatacgttg taagagaacg atgctccggt gtaacgcact tctgtcggaa acagctctgg
    19861 taacagcgca cccatcggcc cgaaggtcag ccccatcaga cttaacccca gcagcaggaa
    19921 ggcaaaaacc agaatcgggt tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa
    19981 caggatgatc agcgtggtga tgatcaccat gcttttacgg cgaccaaagg catcagccag
    20041 taatccggcg actggcacca tcacgccaaa accaataact gccatcatca gcatccacaa
    20101 cacttcgtta cgcggcaggc caagcccaac tggcgcggcg gcggtactga aggtcataga
    20161 gtagaccgtc atgatgtaaa acagcgtata ggttgccagc ataatgaacg tacccagtac
    20221 ggttacgcga acatgtttgg tcagcagcgt acccagcggg atcttcacct gttttttcgc
    20281 tttagcgact ttctcaaaca ccggcgactc atgcagcgac acgcgaacat acaggccgat
    20341 aatgaccagc accgccgaga agataaatgg cacacgccag ccccagctca taaactgctc
    20401 atcggtcagt agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg
    20461 tgcgcctagc tgtgggaagg agccatacag tgcacgtttg cgcggtgggg cgttttcagt
    20521 cgccagcagc gccgcgccgc cccattcacc gcctaagccc agaccctgac caaatcgagc
    20581 cagcgccagc agcagtgggg cgaaaatacc aatcgtggca tagcccggca gcagaccaat
    20641 caccacggtc gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac
    20701 gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga aggcgatggc
    20761 gaaggtggcg agcgactgta gcgttgctgc cgttggatcg ccctgcggga agaagatatg
    20821 cggaaacaca ataacggccg cagtggcgta aatgtaaaag tcgaagaact caatggccgt
    20881 gccaatgaga gaggcgacaa ggactttatt acgcgagttg atcggcgtgt attcttgttc
    20941 gtggtcgagt gttgtggctg ttgcttgcat aatcttttct tattttttgg ctaacgaata
    21001 gccatattac gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa
    21061 aaaccaggca aaaaggggat aatgtttagg ccagtgattt ttcttctatg aaatgcttca
    21121 cataatttta taaatagtgg ataaaactgg tttcaggtta aataaaagtt accaactgga
    21181 tttatatgct ggggttatga gttggagtga aattgtttgc tcccaaatag atggctgaag
    21241 aggctctggg gaagagtttc aagaggttat gtgcgcatta tagccggatg cgatgctggc
    21301 gcatcttatc cggcctacgg ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt
    21361 tacgccgcat ccggcctatt ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta
    21421 tattcggcgg tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa
    21481 aatgccatca gccccagcac ggaaccaaat gctgcgccag agggggattt catcagcgat
    21541 ggcagggtgt aggtcatcac gattttaatc acttcaaaac caatagcagc cagaaatgtt
    21601 ccgcgaatca gggctttttt gcgcggacgg tggcgcggca accgccagaa gatccagaag
    21661 aaaagcagat agttggcgaa gatggaaatc gccaggccaa tcaatcgcca cgtcggtttc
    21721 agccactcaa tgctgttcag gtgcagggcg ctaataatca tttgctgcgc cgaaccggca
    21781 accgaggtga tcgaaagcgt cacaatcagc gcaatcaaca aaccaatcag tgaaataaaa
    21841 tcacgcagat attttaccca gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg
    21901 cgcgactggg cacgaatcgc ttcacgcagg ttacccatcc agttgatgcc ggaataaagt
    21961 gccaccgcca ggccgacaag ccctacagtc gtacgctgct gaacggcggt gttgatggtg
    22021 tttttcaacg tggcggctag tgtcggatcg ctgatgtttt gcagaatttt gtcgaagata
    22081 tcctgtagca acatcggatg ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc
    22141 aaaatcggga tcatcgacaa aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc
    22201 agccgatcgt taaagcgttc tgtcgcgcga ataaggtgcg caataaccgg ttggcgttgg
    22261 actttttcgg cagtggtggt gacggtttcc agcgcctggc taactttgcc gcctttctcg
    22321 ctattatcca actgcttaat cggctcgtgc tccagatcct gggtgggacg tttgatctcg
    22381 ttttcctgcg tcatccgctc ttttgtcctt ctgtcgtggt cattcttaaa aagtatagtc
    22441 ggtcagtcca ggtgctcttt cattacgcca gccagccact gcataaacag gtttacacgc
    22501 cgggaaagct cccgacgctg cgggtaaacc agcgaaaggg agagcggctc ggcacggtag
    22561 cctggcaaca cttcaataag ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg
    22621 atctgaataa tccccagacc ggcaagaccg gcggcgagat agttttcgct actgtttacc
    22681 gtcaacatgc cgccagactt aaaccactgg acgccattaa cgctggcaac ctcaaaacct
    22741 aacggatgta cacccaggtg cggtgtataa cgcactattg cgtgtgaagt cagatcatcg
    22801 gggctttgcg gataaccaaa gcgcgtcaga tagtgcggac tggcacagtt gaccatggtt
    22861 agtttgccga ggggacgggc gataacgcca tcttccggta acgcgccagt gcgtatcacg
    22921 caatcaaaac catcatgaag aatatctacc ggacggttat ggctactcag ttccagctca
    22981 attcccggat gctgatagag aaattccgac agacgcggca gtaacaggtt tttcgcgatt
    23041 cctgacggga tgtcgatgcg taatttaccg ctgatactgg tggcatcctg ttgaaacaga
    23101 ccgtccagtt cgctgagatt actcaacaca tcttttgccc gttgataata ggtcatgcct
    23161 tctggcgtga gtttgacccg tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt
    23221 tccagtgcct gtatctggcg cgaaacactt ccctttggca aagcaaagaa atccgccgcg
    23281 cgggaaaaac tttccagctc cgcgactttg atgaacaact gcattgcgtg aattttatcc
    23341 atttctttga ccgattgttg ttcacagcga aacagtaacg cgcatatcgc attgtttatt
    23401 gttttttcta acatctaatg ccgggtttca caatgttgtc atgacgaatg tggcgatttc
    23461 tcagcaggaa aagggcggaa tagctttatg agaaaggtga atccccttaa acagagcgta
    23521 tttttcagtg attcccttta aagtgaatag aggacgtatt ggctatttgt gcttattttt
    23581 gtctcattgc catttgttat tttggcttgt agtcgcggaa tttcattttt aattgcatgc
    23641 gtactaacca ccaatataag gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt
    23701 aatttcaggc gtttagctgt aactatttat atcggtaact attttctacg cgtcatctct
    23761 gtagaatggc caccgccaaa tggcgaattc ttattttttc tcgcgggtaa tacccttcca
    23821 ctacttaagt ctttttaacg tattcaggtg gagattcagt tatgcaaata gtcatgtttg
    23881 acaggcagtc aatatttatt catggaatga aaatcagttt acagcagcgt attccaggag
    23941 tgagtattca gggggtcagt caggcagacg agttatggca aaagctggaa agttaccctg
    24001 aagccttagt tatgctcgat ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa
    24061 aaaccgtggt gcagtttcct gaggttaagg tgttaatcac ggcgacggat tgcaataaac
    24121 ggtggttaca ggaagttatc cattttaatg tgctggccat tgtgcctcgc gattcaaccg
    24181 tcgagacatt tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt ctgcctggag
    24241 actggcggac gacgccggaa aaggacatta aagatctcaa atcgttaagc gcccgccagc
    24301 gggagatttt aaccatgtta gcagcgggcg aatcaaataa ggagatcggc agagcgctga
    24361 atatcagtac cggaacggta aaagcgcatc tggaatccct gtatcgccgt ctggaagtga
    24421 aaaatcgcac ccaggcggca atgatgttaa atatctcctc c
//
LOCUS       DADWTH010000055        24188 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.055, whole genome shotgun sequence.
ACCESSION   DADWTH010000055 DADWTH010000000
VERSION     DADWTH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.055"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(22..594)
                     /locus_tag="JNY02_002368"
     CDS             complement(22..594)
                     /locus_tag="JNY02_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBB9433885.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JNY02_002369"
                     /pseudo
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JNY02_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNY02_002370"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNY02_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBB9433886.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNY02_002371"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNY02_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBB9433887.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JNY02_002372"
     CDS             complement(3556..5337)
                     /locus_tag="JNY02_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBB9433888.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JNY02_002373"
     CDS             complement(5330..7102)
                     /locus_tag="JNY02_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBB9433889.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIHEEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="JNY02_002374"
                     /pseudo
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="JNY02_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNY02_002375"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNY02_002375"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBB9433890.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLSSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="JNY02_002376"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="JNY02_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="JNY02_002377"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="JNY02_002377"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBB9433891.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /gene="fadM"
                     /locus_tag="JNY02_002378"
     CDS             complement(11173..11571)
                     /gene="fadM"
                     /locus_tag="JNY02_002378"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBB9433892.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="JNY02_002379"
     CDS             complement(11665..12036)
                     /locus_tag="JNY02_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBB9433893.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNY02_002380"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNY02_002380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB9433894.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALGEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNY02_002381"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNY02_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBB9433895.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNY02_002382"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNY02_002382"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBB9433896.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNY02_002383"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNY02_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBB9433897.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNY02_002384"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNY02_002384"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBB9433898.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNY02_002385"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNY02_002385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBB9433899.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNY02_002386"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNY02_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBB9433900.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="JNY02_002387"
     CDS             21807..22385
                     /locus_tag="JNY02_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB9433901.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="JNY02_002388"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="JNY02_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBB9433902.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5670 a         6660 c         5833 g         6025 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgctag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg aagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcactc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gtgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cagcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ctgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gcgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctaatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 cgaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcacgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca cccagcgcca gcagaaccac
    12361 attaccttgc atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgactgtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgtta
//
LOCUS       DADWTH010000056        23748 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.056, whole genome shotgun sequence.
ACCESSION   DADWTH010000056 DADWTH010000000
VERSION     DADWTH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.056"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="JNY02_002389"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="JNY02_002389"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBB9433903.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="JNY02_002390"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="JNY02_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBB9433904.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="JNY02_002391"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="JNY02_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBB9433905.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="JNY02_002392"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="JNY02_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBB9433906.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3874..3998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3880..3924
                     /gene="pheM"
                     /locus_tag="JNY02_002393"
     CDS             3880..3924
                     /gene="pheM"
                     /locus_tag="JNY02_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBB9433907.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="JNY02_002394"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="JNY02_002394"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBB9433908.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="JNY02_002395"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="JNY02_002395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBB9433909.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARCHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEATEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREVDLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="JNY02_002396"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="JNY02_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBB9433910.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="JNY02_002397"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="JNY02_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBB9433911.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="JNY02_002398"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="JNY02_002398"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBB9433912.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="JNY02_002399"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="JNY02_002399"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBB9433913.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="JNY02_002400"
     CDS             10418..10882
                     /locus_tag="JNY02_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB9433914.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="JNY02_002401"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="JNY02_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13331
                     /locus_tag="JNY02_002402"
     CDS             11895..13331
                     /locus_tag="JNY02_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBB9433915.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMPMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNY02_002403"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNY02_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBB9433916.1"
                     /translation="MRYTDSRKLTLETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNY02_002404"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNY02_002404"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBB9433917.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNY02_002405"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNY02_002405"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBB9433918.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="JNY02_002406"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="JNY02_002406"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBB9433919.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="JNY02_002407"
                     /pseudo
     CDS             complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="JNY02_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="JNY02_002408"
     CDS             complement(20164..20457)
                     /locus_tag="JNY02_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBB9433920.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="JNY02_002409"
     CDS             complement(20454..21743)
                     /locus_tag="JNY02_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB9433921.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21769..22736)
                     /locus_tag="JNY02_002410"
                     /pseudo
     CDS             complement(21769..22736)
                     /locus_tag="JNY02_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22756..23520)
                     /locus_tag="JNY02_002411"
     CDS             complement(22756..23520)
                     /locus_tag="JNY02_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBB9433922.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5856 a         5784 c         6261 g         5847 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgttg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagcgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagttgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtct aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgccgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaagcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaatcaatg cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt ggcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 ccccagcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagggg
    22321 ctgttgatct tttcctgagc gaacgctaac ccgccataca tccggtgttc ggcgcaaatg
    22381 tgaccatcga caatatccac cgccgtggca tcgttcacca gcgccgcatt cagttgcaca
    22441 cttaaccgtg ctgccagagc tttaccacgt ttcgtcgcgg ccaacagcaa catagcgggg
    22501 tgtttatctt tcagcagggc agcaatactt tcggcgtaat tttcagtgcg ttgcagcgcg
    22561 tcgttttgct caagaacata aatacatttt ggaccataag gcataactgc ctgcgcctgg
    22621 tcggtatttt gtacaatggc atacacctgt tggccccatt gctgagcgcc gccaaacagt
    22681 tcagcataac gttcaggatt atcgctaaag acccagacgc tgtttaattg actcatcgtt
    22741 tcttctccgt aggcttcagt tcagggcttt cttcagatgt tccgccagct cggcaatggc
    22801 ctccggcgaa tcgttatcga gaatgatgtg cttacgttct gtttgcggcg gtacgcgaat
    22861 gccgaccagt tcagcaagtg gcgcgctctg gctccagtca atatcacttg cctgccactg
    22921 atttaccggt tttttacccg cgccgagaat ggctttcatc gaaggaatgc gtggcacgtt
    22981 aatatcggag gtgacgcaga gcacggctgg aacagagagt tcaataactt caacatcatc
    23041 ttcaagcgtg cgttcaatca ccagtgtatt gccctgacgc tgaatagcac tcactgcatt
    23101 aatcaccgga agttgcagaa tttctccgac cagcaagcca acctgctggg cataaaggtc
    23161 gccggaacct tcaccaaaga tcagtaaatc gaagccgatc ttttcaattg ctgccgccag
    23221 cgcttttgcg gtatcgagag gcagtgcatg ttcaagttgc gcatcctgca ccaaatacag
    23281 gctgtgcggc ccgcgggata gcacgtcttt gcgcactttc gagttctgca acaatgagcc
    23341 gccaacggtc agcgcggcta tctcatcgtc atctgttgcg agctggcttg cagcttcaat
    23401 ggcattgaga tcgaactggc tgattttggc gtcggcattg tcgaaattca gggtgtattc
    23461 tggagtgaca acaatgtcct gttcttcagg caccagctta aagcaggtta ttattttcat
    23521 gatttctcct gtgagtcgca tacggaacca tctccgatgg aaggtaaaaa tggcctgaaa
    23581 atagtgtgcg atagcggtgc ggggggttcg acggagcaag ctgttttcct tgccaacaat
    23641 cttgcaaaaa aaatgcgatt gctaaaacaa aagtgcaatt tgcggcaata tagttattcg
    23701 gtaatgctgc caacttactg atttagtgta tgatggtgat tttaaggt
//
LOCUS       DADWTH010000057        23733 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.057, whole genome shotgun sequence.
ACCESSION   DADWTH010000057 DADWTH010000000
VERSION     DADWTH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.057"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="JNY02_002412"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="JNY02_002412"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBB9433923.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="JNY02_002413"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="JNY02_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBB9433924.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JNY02_002414"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JNY02_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBB9433925.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JNY02_002415"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JNY02_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBB9433926.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFHDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JNY02_002416"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JNY02_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBB9433927.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="JNY02_002417"
     CDS             5281..5739
                     /locus_tag="JNY02_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBB9433928.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLSVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="JNY02_002418"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="JNY02_002418"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBB9433929.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="JNY02_002419"
     tRNA            6791..6875
                     /locus_tag="JNY02_002419"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="JNY02_002420"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="JNY02_002420"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNY02_002421"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNY02_002421"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBB9433930.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNY02_002422"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNY02_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBB9433931.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRAASLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNY02_002423"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNY02_002423"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBB9433932.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="JNY02_002424"
     CDS             complement(10156..13899)
                     /locus_tag="JNY02_002424"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBB9433933.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSCSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNY02_002425"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNY02_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBB9433934.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="JNY02_002426"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="JNY02_002426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBB9433935.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="JNY02_002427"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="JNY02_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBB9433936.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNY02_002428"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNY02_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBB9433937.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="JNY02_002429"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="JNY02_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBB9433938.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="JNY02_002430"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="JNY02_002430"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBB9433939.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNY02_002431"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNY02_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBB9433940.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSTWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="JNY02_002432"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="JNY02_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBB9433941.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23296..23403
                     /locus_tag="JNY02_002433"
     CDS             23296..23403
                     /locus_tag="JNY02_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBB9433942.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5958 a         6183 c         5726 g         5866 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 atagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgt gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat gaccggattt gcgcatactg aatggttggg attgagcgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggac gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagctggct agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct
     6901 caaacgaatc tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg cggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 agccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagcgcacg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact
     9181 gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt
     9901 tacccagcag catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcaac accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg
    11221 ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcaagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag
    14341 gcgatataga aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcata gcaaagcccg cggagaagcc gatgaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt
    15661 tcggaggcag atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgga gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatatatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg
    16381 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg
    16621 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actggtggat accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgca tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18961 tgattttcgc cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac
    19081 aaatcaacac gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca
    20461 caccatccgg ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccagaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgtgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtatggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacatttcc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc
    22561 ctttactggc ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aacaatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt actcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggagagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgcaaaat gttgtcatta
    23281 caacaaggcg gctatatgac gctcgcgcag tttgccatga ctttctggca cgacctggca
    23341 gcaccgatcc tggcggggat tattaccgca gcgattgtca gctggtggcg taaccggaag
    23401 taaggtgaaa acgggcgtca ggctgccgtg atggcaatat gcgcccgaaa ccggccgcag
    23461 gggtaacagc ctgcggccat ttttgtcttt atctcttcca caagtaaata ttggcaaccc
    23521 gagctggaag catcatccag agcactcatg aaacgactgt aacagctgtt gaaaaaccgg
    23581 aatgcactct tcattgcaaa catttcaggg atgaagtgaa gatgaattga gtcgtagtat
    23641 ccacaccaag ccggcgttat ccggtgaggc tcaatgttgc gggggcttta tccctggtgg
    23701 cattggttgc tggaaagaga aaacccccgc acg
//
LOCUS       DADWTH010000058        22319 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.058, whole genome shotgun sequence.
ACCESSION   DADWTH010000058 DADWTH010000000
VERSION     DADWTH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.058"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="JNY02_002434"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="JNY02_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBB9433943.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="JNY02_002435"
     CDS             831..971
                     /locus_tag="JNY02_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433944.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="JNY02_002436"
     CDS             985..1857
                     /locus_tag="JNY02_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBB9433945.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNY02_002437"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNY02_002437"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBB9433946.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNY02_002438"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNY02_002438"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBB9433947.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNY02_002439"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNY02_002439"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBB9433948.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNY02_002440"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNY02_002440"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HBB9433949.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNY02_002441"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNY02_002441"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBB9433950.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="JNY02_002442"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="JNY02_002442"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBB9433951.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVITLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNY02_002443"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNY02_002443"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBB9433952.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="JNY02_002444"
     CDS             complement(14044..15384)
                     /locus_tag="JNY02_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBB9433953.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="JNY02_002445"
     CDS             complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="JNY02_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBB9433954.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="JNY02_002446"
     CDS             complement(17172..17621)
                     /locus_tag="JNY02_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBB9433955.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNY02_002447"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNY02_002447"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBB9433956.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="JNY02_002448"
     CDS             complement(18421..18750)
                     /locus_tag="JNY02_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBB9433957.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNY02_002449"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNY02_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBB9433958.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="JNY02_002450"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="JNY02_002450"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBB9433959.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="JNY02_002451"
                     /gene_synonym="ygdH"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="JNY02_002451"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBB9433960.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5511 a         5916 c         5294 g         5598 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccaag
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac ggtttttcca cccggcccca gaaccacggt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtca ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgattacc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gagcagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttag attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DADWTH010000059        21644 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.059, whole genome shotgun sequence.
ACCESSION   DADWTH010000059 DADWTH010000000
VERSION     DADWTH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.059"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..157
                     /locus_tag="JNY02_002452"
     CDS             <1..157
                     /locus_tag="JNY02_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBB9433961.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="JNY02_002453"
     CDS             227..1033
                     /locus_tag="JNY02_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB9433962.1"
                     /translation="MQTWLNLQDKIIIVTGSASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="JNY02_002454"
     CDS             1043..1450
                     /locus_tag="JNY02_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBB9433963.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="JNY02_002455"
     CDS             1450..1944
                     /locus_tag="JNY02_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBB9433964.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="JNY02_002456"
     CDS             2010..2807
                     /locus_tag="JNY02_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBB9433965.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="JNY02_002457"
     CDS             2818..3606
                     /locus_tag="JNY02_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB9433966.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /gene="sorE"
                     /locus_tag="JNY02_002458"
     CDS             3658..4887
                     /gene="sorE"
                     /locus_tag="JNY02_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBB9433967.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="JNY02_002459"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="JNY02_002459"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBB9433968.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="JNY02_002460"
     CDS             complement(6049..7680)
                     /locus_tag="JNY02_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBB9433969.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNY02_002461"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNY02_002461"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBB9433970.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="JNY02_002462"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="JNY02_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBB9433971.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /gene="espL4"
                     /locus_tag="JNY02_002463"
                     /pseudo
     CDS             12924..15108
                     /gene="espL4"
                     /locus_tag="JNY02_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNY02_002464"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNY02_002464"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBB9433972.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNY02_002465"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNY02_002465"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBB9433973.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNY02_002466"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNY02_002466"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBB9433974.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNY02_002467"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNY02_002467"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBB9433975.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21577
                     /locus_tag="JNY02_002468"
     CDS             21287..21577
                     /locus_tag="JNY02_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433976.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5339 a         5549 c         5469 g         5287 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcagcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccag atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttactg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcgtc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaatggaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ccttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac ttgctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagg cggttgggtt
    21481 ctataagaag gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc
    21541 gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc gcccctgccg tgcctgccca
    21601 agctggagaa gacgcgccag cgtcgcaccc ggcatttttt tcac
//
LOCUS       DADWTH010000060        21504 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.060, whole genome shotgun sequence.
ACCESSION   DADWTH010000060 DADWTH010000000
VERSION     DADWTH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.060"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNY02_002469"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNY02_002469"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBB9433977.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADSEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNY02_002470"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNY02_002470"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBB9433978.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEMEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JNY02_002471"
     CDS             complement(3121..3420)
                     /locus_tag="JNY02_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBB9433979.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="JNY02_002472"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="JNY02_002472"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBB9433980.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JNY02_002473"
     CDS             complement(4632..5069)
                     /locus_tag="JNY02_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBB9433981.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JNY02_002474"
     CDS             complement(5115..5456)
                     /locus_tag="JNY02_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9433982.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNY02_002475"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNY02_002475"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBB9433983.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMLALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNY02_002476"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNY02_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="JNY02_002477"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="JNY02_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB9433984.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     TKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEKEL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="JNY02_002478"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="JNY02_002478"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBB9433985.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="JNY02_002479"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="JNY02_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBB9433986.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13216
                     /locus_tag="JNY02_002480"
     CDS             12686..13216
                     /locus_tag="JNY02_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB9433987.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13328..13864
                     /locus_tag="JNY02_002481"
     CDS             13328..13864
                     /locus_tag="JNY02_002481"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB9433988.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13972..14796)
                     /locus_tag="JNY02_002482"
     CDS             complement(13972..14796)
                     /locus_tag="JNY02_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBB9433989.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="JNY02_002483"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="JNY02_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="JNY02_002484"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="JNY02_002484"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBB9433990.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRSLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="JNY02_002485"
     CDS             complement(18728..19201)
                     /locus_tag="JNY02_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBB9433991.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNY02_002486"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNY02_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBB9433992.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20351..21349
                     /gene="yiaK"
                     /locus_tag="JNY02_002487"
     CDS             20351..21349
                     /gene="yiaK"
                     /locus_tag="JNY02_002487"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBB9433993.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21361..>21504
                     /locus_tag="JNY02_002488"
     CDS             21361..>21504
                     /locus_tag="JNY02_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBB9433994.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5407 a         5487 c         5277 g         5333 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggaat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccatttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcga
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacacc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagaa agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctcgacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc agcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag
    20341 gaagcctagg atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat
    20401 ttcacgcggc gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac
    20461 cgaatccggc gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga
    20521 aaacggcgat atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga
    20581 acagtgggat gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc
    20641 cattgaactg gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg
    20701 gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg
    20761 gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa
    20821 cccgctgatc gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgtcgat
    20881 gttctcttac ggcatgttag aagttaaccg cctggcaggc cgtcagctcc cggtcgatgg
    20941 tggctttgat gatgagggca atttgaccaa agaacctggc gttatcgaga agaatcgccg
    21001 cattttgccg atgggctact ggaaaggttc tggcatgtcg attgtgctgg atatgatcgc
    21061 tactctcctt tccgacggcg catcggttgc cgaagtcacc caggacaaca gcgacgaata
    21121 cggcatttca caaattttta ttgccattga agtggacaag cttatcgacg gtcccacccg
    21181 cgatgccaag ctgcaacgta tcatggatta cgttactacc gctgaacgcg ctgacgaaaa
    21241 ccaggccatc cgcttacccg gccacgaatt tactaccctg ctggccgaaa accgccgtaa
    21301 cggcatcact gttgatgaca gcgtgtgggc caaaatccag gcgttatgag gagataaatc
    21361 atgatttttg gacatatcgc gcagcccaat ccgtgccgtt tgcccgccgc cattgaaaag
    21421 gcgctcgatt ttctgcgcgc taccaacttc aacgtcctgg agccgggcgt tgtcgaaatc
    21481 gacggcaaga atatttatgc gcaa
//
LOCUS       DADWTH010000061        21359 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.061, whole genome shotgun sequence.
ACCESSION   DADWTH010000061 DADWTH010000000
VERSION     DADWTH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.061"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..76
                     /locus_tag="JNY02_002489"
     CDS             <1..76
                     /locus_tag="JNY02_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBB9433995.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..1018
                     /locus_tag="JNY02_002490"
                     /pseudo
     CDS             92..1018
                     /locus_tag="JNY02_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
     gene            1257..1316
                     /locus_tag="JNY02_002491"
     CDS             1257..1316
                     /locus_tag="JNY02_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBB9433996.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1633..1692
                     /gene="ibsE"
                     /locus_tag="JNY02_002492"
     CDS             1633..1692
                     /gene="ibsE"
                     /locus_tag="JNY02_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBB9433997.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="JNY02_002493"
     CDS             complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="JNY02_002493"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBB9433998.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="JNY02_002494"
     CDS             complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="JNY02_002494"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBB9433999.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVHILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="JNY02_002495"
     CDS             complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="JNY02_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBB9434000.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWLYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLIQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7699..8319
                     /locus_tag="JNY02_002496"
     CDS             7699..8319
                     /locus_tag="JNY02_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBB9434001.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPNRKRKDRWMN"
     gene            8383..9621
                     /gene="cca"
                     /locus_tag="JNY02_002497"
     CDS             8383..9621
                     /gene="cca"
                     /locus_tag="JNY02_002497"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBB9434002.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="JNY02_002498"
     CDS             complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="JNY02_002498"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBB9434003.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10696..11064)
                     /gene="folB"
                     /locus_tag="JNY02_002499"
     CDS             complement(10696..11064)
                     /gene="folB"
                     /locus_tag="JNY02_002499"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBB9434004.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11169..11786
                     /gene="plsY"
                     /locus_tag="JNY02_002500"
     CDS             11169..11786
                     /gene="plsY"
                     /locus_tag="JNY02_002500"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBB9434005.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="JNY02_002501"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="JNY02_002501"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12937..13780
                     /gene="ttdA"
                     /locus_tag="JNY02_002502"
                     /pseudo
     CDS             12937..13780
                     /gene="ttdA"
                     /locus_tag="JNY02_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13777..14382
                     /gene="ttdB"
                     /locus_tag="JNY02_002503"
     CDS             13777..14382
                     /gene="ttdB"
                     /locus_tag="JNY02_002503"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBB9434006.1"
                     /translation="MKKILTTSIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14430..15893
                     /gene="ttdT"
                     /locus_tag="JNY02_002504"
     CDS             14430..15893
                     /gene="ttdT"
                     /locus_tag="JNY02_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBB9434007.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYPPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="JNY02_002505"
     CDS             complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="JNY02_002505"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBB9434008.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17187..17402
                     /gene="rpsU"
                     /locus_tag="JNY02_002506"
     CDS             17187..17402
                     /gene="rpsU"
                     /locus_tag="JNY02_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBB9434009.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17513..19258
                     /gene="dnaG"
                     /locus_tag="JNY02_002507"
     CDS             17513..19258
                     /gene="dnaG"
                     /locus_tag="JNY02_002507"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBB9434010.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19453..21294
                     /gene="rpoD"
                     /locus_tag="JNY02_002508"
     CDS             19453..21294
                     /gene="rpoD"
                     /locus_tag="JNY02_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBB9434011.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5157 a         5896 c         5484 g         4822 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact
      421 cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact agccagaccg cagaagccga
      481 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      541 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      601 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      661 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt
      721 gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg
      781 tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac
      841 gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc tctcttaccg
      901 cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggttcact
      961 ggcgacattg acttcaccct taacctcaac aactcccgtc gccgaaaacg tagaataaat
     1021 tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct
     1081 aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa
     1141 ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1201 agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa agggttatga
     1261 tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc
     1321 caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa
     1381 atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt
     1441 atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg
     1501 ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg ctcatcaaca
     1561 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag
     1621 aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact gttaagtttc
     1681 ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt gttcctttac
     1741 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga gatctgtgaa
     1801 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     1861 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     1921 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     1981 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     2041 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc ccctttcagc
     2101 cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag tttgcgggca
     2161 tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt ggtcatcacc
     2221 actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2281 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2341 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2401 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2461 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2521 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2581 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2641 acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta gcgctcaaaa
     2701 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     2761 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     2821 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc gaaaccttct
     2881 tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     2941 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     3001 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3061 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3121 ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga
     3181 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3241 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3301 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3361 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3421 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3481 gctccagaat atgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3541 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3601 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3661 gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca cgacgcaccg
     3721 cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     3781 gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct gcggatgtca
     3841 ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac aaaatgccgg
     3901 aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc
     3961 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4021 taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     4081 ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag cgggccacca
     4141 tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc caggttaagt
     4201 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4261 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4321 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc ggctggtaaa
     4381 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4441 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4501 gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4561 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4621 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4681 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4741 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     4801 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     4861 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     4921 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     4981 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     5041 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgact
     5101 gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     5161 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5221 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5281 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5341 ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc tcctggtaat
     5401 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagtc
     5461 gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc
     5521 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5581 aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattt agttccccgc
     5641 cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5701 gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     5761 tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     5821 gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     5881 cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa tgttgccatt
     5941 cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     6001 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta agtgattcct
     6061 cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6121 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6181 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6241 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6301 tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg caactggtca
     6361 cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6421 agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc tttggcatct
     6481 aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6541 gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt cgcttcgcat
     6601 tgaatcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc accgaacagc
     6661 atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc cgcatcgttg
     6721 ccgcgtaccc acagttcttc atgatacagc cattgcgcta acaccagctc gagcgccgct
     6781 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     6841 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     6901 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     6961 ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc cgctttcact
     7021 tccccctggt cgagagcgat ttcaattcgg ctgccatcga tctctactag ccatttttcg
     7081 cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggc aggcaattcg
     7141 ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7201 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7261 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct acgcagccag
     7321 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7381 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7441 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7501 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7561 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7621 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7681 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7741 agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacga actgaatacc
     7801 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     7861 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     7921 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     7981 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8041 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8101 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     8161 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8221 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg
     8281 atcccaaacc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8341 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8401 ggtggtgctg ttcgggatgc attgttaggg ctatcggtca aagacagaga ttgggtggtg
     8461 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8521 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8581 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8641 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8701 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     8761 gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc
     8821 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     8881 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     8941 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact tcgcgtttta
     9001 ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaagtggca tccggaaatc
     9061 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     9121 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9181 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9241 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9301 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9361 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg
     9421 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaaggc
     9481 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggcgtg
     9541 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa
     9601 cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt cccctcgccc
     9661 cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac aatcaccccc
     9721 accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg aaccttttct
     9781 caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa tggcgaacgg
     9841 gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca gcgccaccac
     9901 aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca ggaagcccca
     9961 gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga
    10021 gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa tggtcgcccc
    10081 ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca taaacgcctg
    10141 acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca ggcattcggc
    10201 ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg ggttaaacaa
    10261 tgacttaatt gtgtcgtgga ataatagccc cagtaccacc gccggaatca tccccagcaa
    10321 aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg ggcggccaaa
    10381 gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg ccagaattga
    10441 tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaatc ccaacaagtg
    10501 accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca atccttcgac
    10561 cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata aacccctaaa
    10621 ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata accgtttggt
    10681 ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc
    10741 aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga
    10801 gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa
    10861 acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc
    10921 cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac
    10981 taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag
    11041 ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac
    11101 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg
    11161 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    11221 tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct
    11281 ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag
    11341 tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg
    11401 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    11461 ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa
    11521 ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    11581 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    11641 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    11701 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    11761 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    11821 tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgaatt
    11881 tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt tgtgcatact
    11941 ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    12001 gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg ctattggagg
    12061 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    12121 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaatg
    12181 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    12241 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    12301 caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact
    12361 caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    12421 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    12481 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    12541 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    12601 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    12661 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    12721 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    12781 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    12841 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt
    12901 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    12961 caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat ttctacccga
    13021 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
    13081 gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
    13141 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
    13201 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
    13261 ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt
    13321 agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc ggaaatcgaa
    13381 gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca
    13441 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
    13501 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
    13561 ctctcgcgta aagccattct gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca
    13621 gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
    13681 accggcaaca gttcagtgat gggcgcatcg tcgcggcacg ctgctggttc atgccgatct
    13741 cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct gacaacctcg
    13801 atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct gaccggtacg
    13861 ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg tccgatccct
    13921 tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa aaacggcgac
    13981 aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag ttttgaacgt
    14041 gaatttattg agcagaccgg cgtaaaactg gtggttggca aaggtggtat ggggccgctg
    14101 accgaagaag gctgccagaa attcaaggcg ttacatgtga ttttcccggc aggctgcgcg
    14161 gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga actcgggatg
    14221 ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt ctctattgat
    14281 acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc
    14341 atcgtggaag agatctgcga gcacgtccat tacatcaaat aaccctcccg gagaggctca
    14401 cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg tggcgatatc
    14461 ttgcaccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca gggctggaga
    14521 accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg atcctcgaac
    14581 ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata ctctctccct
    14641 ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc gccaaatccc
    14701 tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc gccgccttta
    14761 tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg attctggtga
    14821 aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc gagctgatcc
    14881 tagcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat cccatcatcc
    14941 gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc agcattggct
    15001 cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt ttcctgacgg
    15061 cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac gccacgctga
    15121 gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactgctt ctgctggttc
    15181 cctggctggc ttacgtgcta tacccgccgg tactgaagtc tggtgatcag gtgccgcgct
    15241 gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa cggatgctgg
    15301 ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg tgattatatc gatgccgcga
    15361 tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt tgggacgaca
    15421 ttgtcagtaa taaagcggca tggaacgttt tcttctggct ggcctcgctt atcaccctcg
    15481 cgaccggact caacaatacc ggttttatta gctggtttgg caaactgtta gcaggcagct
    15541 taagtggtta tccgccgacg atggtgatgg tgacgttgat tgtggtgttt tatctactgc
    15601 gctacttttt cgccagcgcc acggcgtata cctccgccct cgcaccgatg atgatcgccg
    15661 ccgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt ggcgcggcaa
    15721 ttggtctggg gagcattctt acaccatacg ccaccggacc cagtccgatt tactacggta
    15781 gtggttatct gccaacggcg gattactggc gactgggggc gatttttggg ctgatattcc
    15841 tcgtgttgct ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt taagtgagga
    15901 cggtgccgga aattgctctc cggcacccaa tagttttacg cagccggtaa ctccgccagc
    15961 ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt aaaccgcacc
    16021 attccggcat aggcgatcat cgcgccgtta tcagtacaaa attccggacg cgcgtagaac
    16081 acttcgccgc ggcgtttttt catcatttca gccagcttcg cccgtaacgt gcggttagca
    16141 ctcacgccgc ccgccatgac cagtcgctta aagcccgtct gatccaacgc tcgcttacac
    16201 ttaatcatca acgtatcgac caccgcatct tcaaaggcgc gggcgatatc agcacgcgtc
    16261 tggtcgtcgg tgctgttgtc acgaatcgtg ttcgccgcaa aggttttcag accagaaaag
    16321 ctgaaatcca gccccggacg gtcggtcatc ggacgcggga aaacaaagcg cccggcagta
    16381 ccctgagccg ccattttcga cagtaacggt ccgccaggat aatccagccc cagcagcttc
    16441 gcggttttat caaacgcttc accggcggca tcatcgatag actcgccgag cagctcgtac
    16501 tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggacaccag cagcgcgaca
    16561 aacggaaatt ccggcgggtt atcttccagc atcggcgcta acagatgccc ttccatatgg
    16621 tgtaccggga ttgccggaac gttccaggca aacgccagag agcgccccac ggttgcgcca
    16681 accagcagtg cgccgactaa tccagggcct gcggtatagg ccacagcatc aatgtctttt
    16741 gccgttaaac cagactcctt tagcgccgcc tggatcaacg gtacggtttt acgcacatga
    16801 tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt cacctgacta
    16861 tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc ggtttcatcg
    16921 caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta ttaccgcgca
    16981 gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa gcctcgtgta
    17041 tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta caaagcagca
    17101 gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc cagcggcaaa
    17161 ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa aacgagccgt
    17221 tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt ctggcggaag
    17281 ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct aaagcttctg
    17341 cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact cgtctgtact
    17401 aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg ccgtgcttcc
    17461 gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc ctatggctgg
    17521 acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg tcgatctgat
    17581 cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt gtccattcca
    17641 caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc actgctttgg
    17701 atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc tcgagttcgt
    17761 cgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg aagcaggcag
    17821 cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga tggacggtct
    17881 gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc gccagtatct
    17941 ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt ttgcgccccc
    18001 cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc agtcattgat
    18061 tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt tccgcgaacg
    18121 ggtgatattc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg ggcgcgtgct
    18181 gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc ataaaggccg
    18241 ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc gtctgcttgt
    18301 ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt acgccgttgc
    18361 gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg cgaccaacaa
    18421 tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat gccgcatggc gagcgctgga
    18481 aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt tgccggatgg
    18541 cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag cgcggatgga
    18601 gcaggcgatg ccactctccg catttctgtt taacagtctg atgccgcaag ttgatctgag
    18661 tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat cgcaagtgcc
    18721 gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag gcatacttga
    18781 tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt ctcgccctgt
    18841 tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc aaaatccaga
    18901 attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc ctggacttgg
    18961 cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacct ccgggcaact
    19021 tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt cgatgtggga
    19081 cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca accatatgtt
    19141 tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca cgcatggttt
    19201 aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga aaaagtgatt
    19261 taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact gagaggcagc
    19321 ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg ttggatttca
    19381 gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg aaataagtgt
    19441 ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt cacccgtggt
    19501 aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga agatatcgtc
    19561 gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat tcaggtgatg
    19621 gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga cgaagatgct
    19681 gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg cacgactgac
    19741 ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg cgaaggcgaa
    19801 attgacatcg ctaagcgtat tgaagacggg atcaaccaag ttcaatgctc cgttgctgaa
    19861 tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc agaagaagcg
    19921 cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga tctggcacct
    19981 accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga cgaagatgaa
    20041 gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga cccggaactg
    20101 gcgcgcgaaa aatttgcgga actacgcgct cagtacgttg taacgcgtga caccatcaaa
    20161 gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc tgaagtattc
    20221 aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat gcgcgtcatg
    20281 atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga gcagtgcaaa
    20341 atgccgaaga aaaacttcat taccctgttt accggcaacg aaaccagcga tacctggttc
    20401 aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt ctctgaagaa
    20461 gtgcatcgcg ccctgcaaaa actgcagcag attgaagaag aaaccggcct gaccatcgag
    20521 caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg ccgtgcgaag
    20581 aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa atacaccaac
    20641 cgtggcttgc agttccttga cctgattcag gaaggtaaca tcggcctgat gaaagcggtt
    20701 gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg gtggatccgt
    20761 caggcgatca cccgttctat cgcggatcag gcgcgcacca tccgtattcc ggtgcatatg
    20821 attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga gatgggccgt
    20881 gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa gatccgcaaa
    20941 gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga tgatgaagat
    21001 tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga ttctgcgacc
    21061 accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc gcgtgaagca
    21121 aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct ggaagaagtg
    21181 ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa ggcgctgcgc
    21241 aaactgcgtc acccgagccg ttctgaagtg ttgcgtagct tcctggacga ttaatcggta
    21301 ggccggatca ggcgttacgc cgcacccggc actagtctct ctgcacaaac gccaccttt
//
LOCUS       DADWTH010000062        21254 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.062, whole genome shotgun sequence.
ACCESSION   DADWTH010000062 DADWTH010000000
VERSION     DADWTH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.062"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(32..1012)
                     /locus_tag="JNY02_002509"
     CDS             complement(32..1012)
                     /locus_tag="JNY02_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBB9434012.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSALDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="JNY02_002510"
     CDS             complement(1020..1730)
                     /locus_tag="JNY02_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBB9434013.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNY02_002511"
     CDS             complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNY02_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBB9434014.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4143..5552
                     /gene="glnA"
                     /locus_tag="JNY02_002512"
     CDS             4143..5552
                     /gene="glnA"
                     /locus_tag="JNY02_002512"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBB9434015.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5725..6774
                     /gene="glnL"
                     /locus_tag="JNY02_002513"
     CDS             5725..6774
                     /gene="glnL"
                     /locus_tag="JNY02_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBB9434016.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6786..8195
                     /gene="glnG"
                     /locus_tag="JNY02_002514"
     CDS             6786..8195
                     /gene="glnG"
                     /locus_tag="JNY02_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBB9434017.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8307..8417
                     /locus_tag="JNY02_002515"
     CDS             8307..8417
                     /locus_tag="JNY02_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBB9434018.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNY02_002516"
     CDS             complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNY02_002516"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBB9434019.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNY02_002517"
     CDS             complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNY02_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBB9434020.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11299..11931
                     /locus_tag="JNY02_002518"
     CDS             11299..11931
                     /locus_tag="JNY02_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBB9434021.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNY02_002519"
     CDS             complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNY02_002519"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBB9434022.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15463..16395
                     /locus_tag="JNY02_002520"
     CDS             15463..16395
                     /locus_tag="JNY02_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBB9434023.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16436..17866)
                     /locus_tag="JNY02_002521"
     CDS             complement(16436..17866)
                     /locus_tag="JNY02_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBB9434024.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRIQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNVVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNY02_002522"
     CDS             complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNY02_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBB9434025.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNY02_002523"
     CDS             complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNY02_002523"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBB9434026.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQESPLQLTPMY"
     gene            complement(19727..19996)
                     /locus_tag="JNY02_002524"
     CDS             complement(19727..19996)
                     /locus_tag="JNY02_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBB9434027.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20066..20650
                     /gene="mobA"
                     /locus_tag="JNY02_002525"
     CDS             20066..20650
                     /gene="mobA"
                     /locus_tag="JNY02_002525"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBB9434028.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20632..21159
                     /gene="mobB"
                     /locus_tag="JNY02_002526"
     CDS             20632..21159
                     /gene="mobB"
                     /locus_tag="JNY02_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBB9434029.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5182 a         5350 c         5122 g         5600 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctaaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc catcgctggc
     1921 aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga
     1981 cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt cacttctacc
     2041 agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc
     2101 agaacaacag cttcgtcagt accggaagca cgcatgttgg tcagtttctt accggtcagg
     2161 cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc ttcgtaaact
     2221 tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg
     2281 acagctttac cctgaccgtt agagatcagt acaccgttct gacgctgacc cacttcgccc
     2341 ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg
     2401 gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt
     2461 acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc
     2521 tgcatcacag aaccctgatg ctgttcttca acgtccagcg tcacgttttc atacggctct
     2581 tgtttacgac cgtcgatttc acggaagatt actttcggac gggataccgc cagttcgaaa
     2641 ccttcacgac gcatgttttc gataagaaca gagaggtgca gttcaccacg accagaaacg
     2701 cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct
     2761 ttgttcagac gatcgaggat ctgacgagac gttacgaact taccttcttt accgcagaac
     2821 ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc
     2881 agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc
     2941 gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc cagaccgagg
     3001 tggcccagca ctttaccgac tttcgcgtta cgggttttgc cttcgctatc gatgatagta
     3061 acctgctggt tcggcttcac tttaccacgc ttgatgcggc caatgccgat aacgccaaca
     3121 tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc
     3181 ggcgcaggaa cgtggtcaac aatcgcctga tacagcgggg tcatgtcttc cgccatatct
     3241 tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc
     3301 tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca
     3361 ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca
     3421 aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc
     3481 agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc
     3541 cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg
     3601 aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt
     3661 tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccaa ggtggtttta
     3721 ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct
     3781 caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac
     3841 aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa
     3901 aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat tgaagcacta
     3961 tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag catgataacg
     4021 ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac
     4081 ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag gagagttaaa
     4141 gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt
     4201 tgcgcttcac cgataccaaa ggtaaagaac aacacgtcac tatccctgct catcaggtga
     4261 atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag
     4321 gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt
     4381 tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag
     4441 gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg cgttccactg
     4501 gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc
     4561 gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga
     4621 actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt
     4681 acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg tgtctggtga
     4741 tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga
     4801 acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca
     4861 aatatgtcgt gcataacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac
     4921 cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg
     4981 ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg
     5041 gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt
     5101 ataagcgtct ggtcccgggc tacgaagcac cggtaatgct ggcttactct gcgcgtaacc
     5161 gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac
     5221 gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc
     5281 ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc
     5341 tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga
     5401 acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg gatgaagcga
     5461 tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc
     5521 cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaca
     5581 acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc ttttttctcc
     5641 gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca ctaaaatggt
     5701 gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc tgggcagatc
     5761 ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat ccattacgcc
     5821 aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg tacgccgtta
     5881 ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag tctggaggcg
     5941 gggcaaggct ttaccgataa cgaagtgacg ctggtcatcg atggtcgctc gcatatcctt
     6001 tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc accgatggat
     6061 aaccagcgcc gcttaagcca ggaacaacta caacatgctc aacaagtagc tgcccgtgat
     6121 ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gcggtttacg tggcgcggcg
     6181 cagttactca gcaaagcgtt acctgaccca tcactactcg aatataccaa agtgattatc
     6241 gaacaggcgg accggctgcg aaatttggtc gaccgtctgt tggggccgca gctgcccggt
     6301 acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg
     6361 gaactgccgg acaacgtgcg gttgattcgt gattacgatc ccagcctgcc ggaactggcg
     6421 cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg
     6481 ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca actgacctta
     6541 cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg gccgggcatt
     6601 ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga aggtggcacc
     6661 gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa aattgaattt
     6721 accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa ataaaggtga
     6781 cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc cgttgggtgc
     6841 ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac ggcgcggaag
     6901 tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg
     6961 gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg cttccggtca
     7021 tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa caaggggcgt
     7081 ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta
     7141 tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc ccaacgaccg
     7201 atatcatcgg cgaagcgcaa gccatgcagg acgtgttccg tattatcggt cggctttcgc
     7261 gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa ctggtcgctc
     7321 atgccctgca tcgccacagt ccgcgcacca aagcgccgtt tatcgcgctg aatatggcag
     7381 ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc gcgtttactg
     7441 gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggcacg ttatttctcg
     7501 acgaaattgg agatatgccg ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg
     7561 gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg
     7621 ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc
     7681 accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc
     7741 cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt
     7801 tgctgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc
     7861 agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa gtgttgattc
     7921 aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg caaatgcaac
     7981 cggacagttg ggcgacactt ttagcgcagt gggcagacag agcgctgcgt tccggtcatc
     8041 aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg accgcgttgc
     8101 gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc cgcaacactc
     8161 tgacgcgtaa gttaaaagag ctggggatgg agtgattcac ggcttgtgtg taaagattga
     8221 ttattgagcg caaattgctg gtattttgcg ctttactgtt ccgataagtt cagtatgatc
     8281 ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg gtatcgagtg
     8341 gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt gccgcgatga
     8401 ttgggctgtt tagctgaagc tcgttgttta tgccggatgc ggagaaagtc gtaggcctga
     8461 taagacgcga ccagcgtcgc atcaggcatc atgctcagat gcctgatgcg gcgtaaacgc
     8521 cttatccggc ctacaaaagc aataatttca acaggttttg ttgcccagcg catcaggcaa
     8581 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
     8641 actctttaaa tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca
     8701 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
     8761 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
     8821 aagtaatcag cgaagtgcaa atcccactgt ttctcaagag gggcgtaatc cagacggaag
     8881 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
     8941 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
     9001 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
     9061 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
     9121 gccaccgcca gctcgtcatc aggacgggca aagtgatcca taccgataaa ctgatagccc
     9181 gattgcgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
     9241 aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta
     9301 aaaacgctca gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc
     9361 tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc
     9421 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
     9481 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg
     9541 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc
     9601 gcatcggtat tgaaatggaa gttttcgcgc agcagcttca tcagacggct gatttgcgct
     9661 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
     9721 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
     9781 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
     9841 gggatatgta cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac
     9901 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
     9961 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    10021 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg
    10081 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    10141 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    10201 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    10261 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    10321 gtttagcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    10381 ccagcagcgc atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa
    10441 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc actcccaatg
    10501 gaataggggt tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg
    10561 acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    10621 gcttacggtc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    10681 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    10741 tattcagtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    10801 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    10861 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccgt
    10921 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    10981 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    11041 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    11101 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    11161 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    11221 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    11281 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    11341 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    11401 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    11461 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    11521 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    11581 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    11641 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    11701 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    11761 cgtaaagcgc tattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    11821 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtagaca agctgcggca gaaactggat
    11881 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    11941 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    12001 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    12061 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    12121 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    12181 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    12241 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    12301 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    12361 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    12421 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    12481 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    12541 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    12601 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    12661 gcccagctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    12721 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    12781 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    12841 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    12901 gatatctttt ccttccgcga atgcggtcag caagccttta tcacgcgaga gatgcgccat
    12961 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    13021 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    13081 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    13141 cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgact tcagcttcgc
    13201 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    13261 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg
    13321 tttttcaaac agaatggttt gcaactgctt ggtggaagaa aggttaaatt cttcacctgc
    13381 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagaatgatt
    13441 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    13501 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    13561 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    13621 ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg caatctcttc
    13681 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    13741 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc
    13801 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    13861 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgtgag agatttgatc
    13921 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    13981 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    14041 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    14101 gatagtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    14161 aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    14221 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    14281 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacatcggt
    14341 tttaatcgtc gccaactggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    14401 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    14461 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    14521 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaagtcga tgatcaattc
    14581 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    14641 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctaat
    14701 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    14761 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg
    14821 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    14881 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    14941 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    15001 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    15061 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    15121 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    15181 gaaaatcttt tgtagatctg ctggatcgct cgcaaatcgt catgtggata actttgtgca
    15241 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    15301 tatttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg ggattttttc
    15361 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcattt
    15421 tctgctaaaa tctgcgcctt gcgcttcttt agtgcactgt ctatggctaa ccttttgaat
    15481 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    15541 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    15601 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    15661 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    15721 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    15781 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    15841 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    15901 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtcaa
    15961 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    16021 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
    16081 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    16141 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
    16201 ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt acagcctatt
    16261 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    16321 tggctgaact cattgtggca ggaaaaagac cggcttctga cgtctctcat atcatcacag
    16381 cgtcagaata agtaagtttt actcgcctct gaatgagcag aggcgagtga gtattttaat
    16441 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    16501 caactttacc cggtgtataa cgcagctgca gcgaggcggt attgttgtca atcgtgatta
    16561 actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg
    16621 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    16681 taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg gatgatgaag
    16741 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    16801 tccatcttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
    16861 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
    16921 cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc actgctgagg
    16981 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    17041 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    17101 ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    17161 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    17221 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    17281 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
    17341 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    17401 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    17461 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactacgttc acttttgccg
    17521 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    17581 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    17641 tatcgaatgt catttgcatt tgaatacggg aattaaaaaa gcctttgtca aagtttttat
    17701 aacttaacgt catatcgtag gcatgttcac ctttcttttt cgcatcgtta aaatctttaa
    17761 taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    17821 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    17881 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc ggcaggatgc
    17941 attatcagaa tgtatttata tttattaaat aataaaaaaa gcccgtgaat attcacgggc
    18001 tttatgtaat ttacattgaa ttattttttc tcggacagat atttcaccgt atcagcatac
    18061 tgctgaacaa aaacatccat attgctggta tccataccct gcggattcag ctgatattta
    18121 ccgttaacaa acatcgccgg aacaccacgc aattgcacat cagctgcggc tttttcctgc
    18181 tgagcgacca gagatttcac cacgaagctg ttccacgccg cgtcgtactc ttcaccttta
    18241 atacctgcat tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc
    18301 tgtacgcctt caaacagcgg aacagtcact ttgtcttcca cgcccagcgc catcgccaca
    18361 gcccatgcct gagtcagatc tttgcccagg tcaccaccca tgaagttgac gtggtattta
    18421 gtcatcttca cgccttccgg cagttttttc ttcacattat cagaaatatg cagaacttct
    18481 tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca
    18541 gctaccggtt tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgca
    18601 ctaaacgcta aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc
    18661 cgattaatac ataggtgtta attgcaaagg ggattcttgt agaacttttg cctgttctat
    18721 aaaagtcgcc gtctgtcgta gccagtaatc ttccccggtt aaccacggga aatttttcgg
    18781 gaacgcggga tcagcccaat gccgcattag ccaggcaaga taataaacca aacgcatggc
    18841 gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc
    18901 ttcaataata gtttccagtt gcatccgctg ctgggcttta tcgccattga gcaacatcca
    18961 caaatcctga atagctggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc
    19021 gcgccagaga atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc
    19081 ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc gctttcagaa atgccgcttt
    19141 caacccggaa ggtatcagtg tagcgtcctc aaacagcttg cgaggctcaa tgagatattc
    19201 attcagaccg atggtcgggc gatgaataaa aagctgtttg cgccccgtct ggtgcatacg
    19261 ccctaaataa cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc
    19321 accgacgctt ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt
    19381 aaaggccaca ggtgctgcga ccggaacttc atcatttacc agctgcaacg caaattgatg
    19441 ttcttcgagg atttgatcgg ctgtccaacg ttcagggcga taaaatttga cgacaaaacg
    19501 tcgacgctct tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag
    19561 accggaatcc acccggatcc cttgctcaaa cagagcgtcc atgatggtat ccgggtgtag
    19621 tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg
    19681 aaggatatca tttcacgatg attttggtgg cgccgcttac aagcttttac tctttaatta
    19741 cgccgcgagc gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg
    19801 ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg
    19861 tcaaatccgc cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca
    19921 acaagttaaa gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca
    19981 gacgtttaca tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa
    20041 tcccacaata aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct
    20101 ggcaggcggt aaagccagac gaatgggcgg cgtagataaa ggattgctgg acttaaatgg
    20161 caaaccgctg tggcaacatg tcgctgacgc gcttaagacg cagctctctc acgtcgtggt
    20221 taatgctaat cgtcatcagg aaatctatca ggcaggcggt ctgaaagtga ttgaagattc
    20281 actggcggat tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagctgg
    20341 tgagtggttt ttgttttgtc cgtgcgatac gccttacatt ccccatgatt tagccgcccg
    20401 gcttaatcat cagcgcaaag atgcgcctgt cgtgtgggtc catgacggtg aacgcgatca
    20461 cccgactatt gctctggtaa accgcgctat tgagccttta ttactggaat atctgcaagc
    20521 aggagaacgc cgggtaatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag
    20581 cgatcataaa gatgcatttg ttaacgtgaa tacgccagag gagcttgccc gatggcagga
    20641 aaaacgatga taccgttact cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt
    20701 ttgaaaaaac tgatcccggc attatgcgcc agagggatcc gtccggggct gattaagcat
    20761 acgcaccatg atatggatgt tgataagcca ggcaaagata gctatgagct gcgcaaggct
    20821 ggcgcggcac aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca
    20881 gacgaagaag agctggatct acattttctc gcaagtcgaa tggatacctc aaagctggat
    20941 ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat
    21001 gcagccggac atcgacctga agaattagtg atagacaggc atgttattgc tgtagccagt
    21061 gatgtgccgc ttaatcttga tgtcgcatta ctggatatta atgatgttga ggggctggct
    21121 gattttgtgg tggagtggat gcaaaagcag aacggataag caagagtagg ctggatcaga
    21181 cgcagccagt gtcgcatccg acctttttct gcaccagcgc aaaaaggccc gactgggcct
    21241 tctgcttaat ttga
//
LOCUS       DADWTH010000063        21037 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.063, whole genome shotgun sequence.
ACCESSION   DADWTH010000063 DADWTH010000000
VERSION     DADWTH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.063"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..3093)
                     /locus_tag="JNY02_002527"
     CDS             complement(<1..3093)
                     /locus_tag="JNY02_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBB9434030.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLCMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     Y"
     gene            complement(3100..3273)
                     /locus_tag="JNY02_002528"
     CDS             complement(3100..3273)
                     /locus_tag="JNY02_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434031.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            3391..4584
                     /locus_tag="JNY02_002529"
     CDS             3391..4584
                     /locus_tag="JNY02_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB9434032.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKTTQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4782..6035)
                     /gene="glyA"
                     /locus_tag="JNY02_002530"
     CDS             complement(4782..6035)
                     /gene="glyA"
                     /locus_tag="JNY02_002530"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBB9434033.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6362..7552
                     /gene="hmpA"
                     /locus_tag="JNY02_002531"
     CDS             6362..7552
                     /gene="hmpA"
                     /locus_tag="JNY02_002531"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBB9434034.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7597..7935)
                     /gene="glnB"
                     /locus_tag="JNY02_002532"
     CDS             complement(7597..7935)
                     /gene="glnB"
                     /locus_tag="JNY02_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBB9434035.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7996..9330)
                     /gene="glrR"
                     /locus_tag="JNY02_002533"
     CDS             complement(7996..9330)
                     /gene="glrR"
                     /locus_tag="JNY02_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBB9434036.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9320..10033)
                     /gene="qseG"
                     /locus_tag="JNY02_002534"
     CDS             complement(9320..10033)
                     /gene="qseG"
                     /locus_tag="JNY02_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBB9434037.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10198..11625)
                     /gene="qseE"
                     /locus_tag="JNY02_002535"
     CDS             complement(10198..11625)
                     /gene="qseE"
                     /locus_tag="JNY02_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBB9434038.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12183..16070)
                     /gene="purL"
                     /locus_tag="JNY02_002536"
                     /gene_synonym="purI"
     CDS             complement(12183..16070)
                     /gene="purL"
                     /locus_tag="JNY02_002536"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBB9434039.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16328..17884
                     /gene="mltF"
                     /locus_tag="JNY02_002537"
     CDS             16328..17884
                     /gene="mltF"
                     /locus_tag="JNY02_002537"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBB9434040.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17881..18417)
                     /gene="tadA"
                     /locus_tag="JNY02_002538"
     CDS             complement(17881..18417)
                     /gene="tadA"
                     /locus_tag="JNY02_002538"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBB9434041.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18442..19077)
                     /gene="yfhb"
                     /locus_tag="JNY02_002539"
     CDS             complement(18442..19077)
                     /gene="yfhb"
                     /locus_tag="JNY02_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBB9434042.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19286..20134
                     /locus_tag="JNY02_002540"
     CDS             19286..20134
                     /locus_tag="JNY02_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB9434043.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVVQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20473..21027)
                     /gene="pinE"
                     /locus_tag="JNY02_002541"
                     /pseudo
     CDS             complement(20473..21027)
                     /gene="pinE"
                     /locus_tag="JNY02_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5054 a         5683 c         5354 g         4946 t
ORIGIN      
        1 gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc aggccggatt
       61 aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga gtcccgcgga
      121 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
      181 cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
      241 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
      301 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
      361 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
      421 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
      481 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
      541 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
      601 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
      661 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
      721 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
      781 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
      841 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttt
      901 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
      961 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
     1021 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
     1081 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
     1141 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
     1201 gtccagtgcg ttcagcatct ctgccgccaa ggtgggcatt ccccagttaa tcagttgccc
     1261 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
     1321 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
     1381 acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
     1441 cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
     1501 aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca gaccataata
     1561 gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
     1621 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
     1681 gtttttgttc agcgcatgtg cgcgtttcag cgcctgactg gcatacgcca ccgcttgcgg
     1741 gaaatctgcg cggttatatt ccagcatcgc cagcgccagg ttacagcgat aatccagcgg
     1801 gtccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt gatgatattg
     1861 ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt gtgctgccag
     1921 tggcgctttg gcgacgtccg gcaacggcaa ctcctgcggc tgatgttcct gataactcag
     1981 tacaatattg ccgtcggcat cggagagttc aatggtcagt ctgtcaggat tgataccgtg
     2041 caacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga gcaacgcgtt
     2101 gcatttgccg atttcgcgga tcgccagacg gtatccgttc aacggagaga tggcatacag
     2161 cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc gggaggcatt
     2221 ttgcaccatg cacagagaat gataaggcag gaaatactgc tcgaaacgct tctcttcgta
     2281 agcatcaagc caggtaaaat caggctggtt atcggcaaaa ataccggtca tcagttcgat
     2341 atacgggccg ttattgtcgg tcagactctt atcccacgcc tggccaaatt cactgtgtcc
     2401 ccaactccac tgttttttac ctggcgcaat atggtggttg gcaacgtgca gcaaaccgcc
     2461 atcttcatcg tgacaccacg cgccgacaaa atcgtactgc gatttttcgg ccatatatga
     2521 ggttggaaca ggcacatttt tatagcgaga aatgtccact ccggcggagt agtccacttt
     2581 gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt gatcaaacac
     2641 cgcagtcaca tccggtggga aaacgctctg atgcccttca ccccctttca ctgccgggtt
     2701 ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc tggcgatttc
     2761 cagtgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac catgcatcgg
     2821 ctccgtttcg ccgacccaca ccgtctgtgc accgtcttca tgggcttcga gggtgaaatc
     2881 aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa tcccgccgga
     2941 gatccacggt cccagcagcc ccaccagcgc aggtttaatg acttcattgt gatagacaaa
     3001 atcgcgctgt ttcactttat cccatgcgcg atgcacccga ccgcccagtt ccggcaggat
     3061 catcactttg atgtagtcgt tttccagcca caccgcctgc taggatttca gggttttctg
     3121 ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc cctgataaac
     3181 gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg tcgggatctc
     3241 aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa caagacatac
     3301 tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg cggaataatc
     3361 acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa tcaacagatt
     3421 cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg cgccaataaa
     3481 tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat tttgcaggaa
     3541 ctggaaagcg aaaaacgggt ggtgaatatt gacgatgaaa gccagacgcg cgggcatagt
     3601 agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgcctgaa cgtgacgccc
     3661 accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggcga atttgagtat
     3721 ttacagattg atgtaccgac gccgcaggcg ctgctgtccg aaatcgaaaa atgctggcat
     3781 cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat ccacgggcag
     3841 gttgatccgg tgaccggcgt atcgcaaacc atgccgcaag cgccgtggac aacgccagtt
     3901 gaggtgaagt atctgctgga agagaagctg ggcattcggg tgctggtcga taatgactgc
     3961 gtgatgctgg cgcaggcgga gaaatggcaa aataattcgc aggaacggga tttctgcgtg
     4021 atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat ttatcgcggc
     4081 agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg cgtcgtctgc
     4141 gactgtggac gttacggctg cctggaaacc gtcgcctcgt taagtgcatt aaaaaaacag
     4201 gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga aaaactgaca
     4261 accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggatcaccag ctgggttgat
     4321 cgctctgcca atgccattgg tttgagtctg tataacttcc tcaacatcct caatattaat
     4381 cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa tactattatt
     4441 cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa aacgacgcaa
     4501 attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta tttgtatgtt
     4561 gaggcgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc cgaaggaagc
     4621 cttgatgatc ataaacgaaa aattgcctga tgcgctacgc ttatcaggcc tacacggaga
     4681 ttgcaatata ttgaatttac aagattttgt aggcctgaga aggtgttcat gccgcatcca
     4741 acatgaacaa cgagcacatt gtcagcaaat caccgtttcg cttatgcgta aaccgggtaa
     4801 cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga taacggcttc atcattgatg
     4861 ctgtccagca cgtcacacat ccagccagcc agttctttcg cttcagcttc tttaaagccg
     4921 cgacgggtaa tcgccggagt acctacacga ataccggagg tcacaaacgg gctcttcgga
     4981 tcgttcggta cgctgttttt gttgacggtg atgttagcac ggcccagagc ggcgtctgct
     5041 tctttaccgg tcaggttttt atcaaccaga tcaaccagga acaggtggtt atcagtgccg
     5101 ccggaaacca ctttgtagcc gcgctcgagg aacacttcta ccatcgcttt agcgtttttc
     5161 gcgacctgct gctggtaagt tttgaactca ggctccatcg cttctttcag agcaaccgct
     5221 ttaccggcga ttacgtgcat caacggaccg ccctgaccac cagggaaaac ggcagagttc
     5281 agttttttgt acagctcttc gctaccacct ttcgccagga tcaggccgcc gcgcggaccc
     5341 gccagggttt tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag
     5401 acgccagcag caaccaggcc cgcaacgtgc gccatatcaa cgaacagata agcaccgatg
     5461 ctgtcagcga tttcacgcat tttcgcccag tcaaccacgc cggaatatgc agagaaacca
     5521 ccgataatca ttttcggctt gtgttctttg gcttgttttt ccagatcggc gtaatcgata
     5581 tgaccggtag catcgatacc gtaaggaacg atgttgtaca gtttaccgga gaagttaacc
     5641 ggagaaccgt gagtcaggtg accgccatgc gccaggttca tacccagaac ggtatcacct
     5701 ggttccagca gcgcggtgta gaccgcaaag ttagcctggg agccggagtg cggctggacg
     5761 ttagcgtagt cagcgccgaa cagttctttc gcacgatcga tcgccagttg ttcaacgata
     5821 tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc atatttgttg
     5881 gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc
     5941 agttcgatgt gctcttcctg acgtactttt tcctgctcca tagcctgcca cagttcggca
     6001 tcataatcgg caatgttcat ttcacgcttt aacatccgca tctcctgact cagctaacaa
     6061 taaaattttt ggcctttata ggcggtcctg ttggacaacg gcgaacagta taaccgaatc
     6121 attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa cgattacctt aaggctacgc
     6181 aaggctttgg agaataaaga gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg
     6241 caaatttttc acttcatcac attctttctg aaaaacacca aagaaccatt tacattgcag
     6301 ggctattttt tataagatgc atttgagata catcaattaa gatgcaaaaa aggaagacca
     6361 tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac
     6421 ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa
     6481 agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc
     6541 tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa
     6601 aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca tcgtcggtga
     6661 acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg
     6721 gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg aaatctataa
     6781 cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca ttgtggctaa
     6841 aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt
     6901 ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca
     6961 tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtatcgc
     7021 ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg ccaatgttgg
     7081 cgatgtcgtg aaactggtcg ctccagcagg tgatttcttt atggctgtcg cagatgacac
     7141 accagtgacg ttaatctcta ccggtgttgg tcaaacgcca atgctggcaa tgctcgacac
     7201 gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga
     7261 agttcacgcc tttgctgatg aagttaagga actggggcag tcactgccgc gctttaccgc
     7321 gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt ttgatagcga
     7381 aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta
     7441 tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg atctgggcgt
     7501 gaagcaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt aatttgatgt
     7561 tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt cgtcctcttc
     7621 accggtacgg atgcgaatga cccgagccac gtcaaagacg aagattttac cgtcaccgat
     7681 tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa tgtcgtccgg
     7741 tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg cgccgcggta
     7801 cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg tcatgccggt
     7861 aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt ttataatcgc
     7921 atcaatcttt ttcatgctat tccttgaaaa ggttgcctgt cttttgatct gctaaacgta
     7981 acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc gggaaagcag
     8041 tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga cgttgccttt
     8101 ggtgatttgc agcagcttac gcaaatagtt gagttcaaaa tgattacgtg cctcaacaaa
     8161 ggttggcagc gccgtatttt caccctccag cgcctgctcc accagcgcat cactaatcac
     8221 cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc gcacattacc
     8281 cggccagctc gcggtcatca ggcgtttcat cgcatcggta gagaacgcgc ggacaaacgg
     8341 tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa tgtcttctgt
     8401 gcgctccgcc agtgccggaa ttttcaggct gacaacgttg aggcggtaat agagatcttc
     8461 gcggaattcc ccgcgcgcca tcgcttttga cagatcacgg tgagtggcag aaataatccg
     8521 cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct cctgcaacac
     8581 gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga gaaatagcgt
     8641 tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag taaacgcgcc
     8701 acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc cacagttaat
     8761 agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg cgaaaatctc
     8821 tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg ccaccagccg
     8881 cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct cgcgccagcg
     8941 ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat atagcgcatc
     9001 tttatcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag caacggcatc
     9061 ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca ctttctggat
     9121 ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga taactaaatc
     9181 tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg tgaccacact
     9241 gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat cgtcatcgac
     9301 caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg gatggcgcgg
     9361 gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg cgggtcgaga
     9421 gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc agttgctgct
     9481 gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac tgctggagtt
     9541 tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg tcgcgccaga
     9601 gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca atacgcgcga
     9661 ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc ccttgtttaa
     9721 aggtattttg ccagctgccg tcgtcatatt ggcgagcttg ctgacgcgac tgcgcaggca
     9781 ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt tcggttgatt
     9841 taccttgcag cgcccagatg tcactacatt cagtagaaag atagtcagcc agttgataaa
     9901 ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc tgcacgcaac
     9961 ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat aatcgttgaa
    10021 aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat tgttgcagac
    10081 agtctggaca tggacagcgc ggagaaaccg gggcgtacat atcgtacgtg aagatttcga
    10141 gcactacccg gggccgaact gacaaataaa atagcctgat aggataggct gttagcatta
    10201 tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc cgctctcgtc
    10261 gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc ttaatcccag
    10321 accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg gttcgaagat
    10381 catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa tataaacccg
    10441 cgcaccatgt aaactgctgc gaaggcaaat gttaccggat tcagccccgt agtgcaccgc
    10501 attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca ggcaagctgt
    10561 tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc tatgagcaga
    10621 aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg cactgtccgc
    10681 ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc agctgctgct
    10741 atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa cctgatcagc
    10801 cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat gagataaatg
    10861 tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta accaaagaat
    10921 acgttgccca accgagcgta actcgctcgg tccacagaac gaaacgctat tgcccagaga
    10981 acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc cgataatcat
    11041 ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg attgccaacc
    11101 aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag agaacaccac
    11161 tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa gacgcgcggc
    11221 ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt tcaaatcctg
    11281 acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat cgagcatttc
    11341 gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt ccagcacgca
    11401 atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt cactacgccg
    11461 ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg cattcaggct
    11521 ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa atgccagcat
    11581 taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt tactctcgtc
    11641 agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag ccgggaatta
    11701 cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga tggttgatat
    11761 agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat tcgaatttta
    11821 tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct tactattatg
    11881 cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg ttgctttgag
    11941 agatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct aaaaagagac
    12001 aacttaagat aaacttatta gattacaata aaatcaatca tttaagtgtc gccagaaagc
    12061 gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc ggagttggga
    12121 gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa tgaactgatg
    12181 acttacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc atcctcgccc
    12241 cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg gtgcggcatc
    12301 ataatggtga ctcgaccact ttcagtcgta accgccgtaa taccgttcgg tgaaccgttc
    12361 gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg cagtgccacc
    12421 agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac gcgcccttca
    12481 ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacag cagagacggg
    12541 ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt gttacgcaca
    12601 aaacgtggcc acaactcgct ccccgggatc agttcacgca ggttagacat catctggcaa
    12661 ccgttacata cccccagggc cagcgtttgc ggacgatgga agaaggttgc aaactcatcg
    12721 cgtacacggt cattgaacag gatagacttc gcccaacctt caccggcacc cagcacatca
    12781 ccgtaggaga aacctccgca cgcgaccagg gcgtgaaaat cctccagtcc cgtacgtcca
    12841 gtcagcaggt cgctcatatg cacgtcgata gcatcaaagc ccccacgttg gaaagctgcc
    12901 gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt cggacgtgcg
    12961 ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag ttttacattc
    13021 aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca ctccgggttg
    13081 tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa cgtagtgcgg
    13141 ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc ggaaaccgcc
    13201 tgcccaacat aatggacgca atccgcaagc ccatgctgtg ccagtacgga ctcgaccgct
    13261 tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa caacgccgcc
    13321 aggcgatcgt cacccagaga ggcgatatcc gcgtcaatgc cacaatgacc tgcaaaggcc
    13381 atttccgcca gcgttaccag caggccgcca tcagagcggt cgtgatacgc cagcagctta
    13441 cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttggac aacatcgcgt
    13501 acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt tgcgcccagc
    13561 gcgttattgc ctttgcccaa atcaatcagc agcagtgcgt tatcttcggt acaaagctgc
    13621 ggcgtgatgg tgtgacgtac atcttccacg cgggcaaatg cagaaatcac cagcgacagc
    13681 ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat cgacatagag
    13741 tctttaccca ccgggatcgt caggcccagc gccggacaaa gttcttcacc cacggcttta
    13801 acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat ccagttggcg
    13861 gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt taacgcttca
    13921 ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc acgctcgcca
    13981 atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc gcagttagcg
    14041 accggcacct gccacggccc caccatctga tcgcgcgcca ccatgccggt tacgctgcgg
    14101 tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag tacgcgtttc
    14161 accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc tttcagcgtt
    14221 tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg cagatcgatc
    14281 ggctgattat caaaatgacg atcgtgcaga gaaagatgca gttcttcggt cgcttcacca
    14341 atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg taattgatcg
    14401 gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc cagcgggctc
    14461 atgcccggtt cgtcgcttag aatatcgcgc agttcaaatt taccgccgcg tccgccgtcg
    14521 ctcaccagct ccggcatcgc gttagaaaga ccgcccgcac cgacgtcgtg gataaacaga
    14581 attgggttag catcaccaag ctgccagcaa cggtcgatca cttcctggca acgacgctcc
    14641 atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga ctgaccagac
    14701 gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc gccgagaacg
    14761 accagcttcg caccgacgtt gatctcgcct ttttgtacgt gatcggcgcg aatgttgccg
    14821 atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc attgtggctg
    14881 ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc aaattcgttg
    14941 ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt aacaatgcgc
    15001 tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caggaattcg caggttggat
    15061 acggagaaac caaccaaacc agctttcggc ttcgcgccgc gcccggtggc accttcatcg
    15121 cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc cgtcgggtgg
    15181 ttgtgagttt cgactttcat caggatgtgc gccggttcct gatggaaatt gtagcggccc
    15241 gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc ggcgttatct
    15301 ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat catcctgaac
    15361 agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat tttgtggcgg
    15421 cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg gttacgacca
    15481 agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc caggccaaga
    15541 cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac gcttgtgacc
    15601 ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa aaagaccgtt
    15661 tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt ggtcagcgta
    15721 ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg tagcccgcag
    15781 ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg acgcggggtc
    15841 accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata tttcagcagg
    15901 cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc aaaatggaca
    15961 tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc cagcagtttg
    16021 ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat aagtctctcg
    16081 tcttctaagc tttcggtgta cccaaggggg ggaaacgggc gtcattataa agaatctgat
    16141 gcgctgacga aaccgtttgc gtggaaataa aatcaccatc gtgaattagc aacgcgtgcc
    16201 gccaatggct gtaataagtt gccatctggc gcaggtttac gcaaaatgcc gctcatttat
    16261 gagtaaacct ttcactatta ttacgttttt tcaagctggg acgcgcacga cacagagaat
    16321 taactaattg aaaaaattaa agattaatta tctgttcatc ggcattctgg cactgctgct
    16381 cgcggtcgct ctctggccat ccattccctg gtttggtaaa gccgacaacc gtatcgccgc
    16441 cattcaagcg cggggagagt tgcgtgtgag caccattcat actcccctga cttataacga
    16501 aatcaacggg aaaccttttg gcctggatta cgaactggcg aaacagtttg ccgattacct
    16561 cggcgtaaaa ctgaaagtga ccgtgcggca gaatatcagc cagctgtttg acgaccttga
    16621 taatggtaac gccgacctgc tggcggcagg actggtctat aacagtgagc gggtaaaaaa
    16681 ttatcagcct ggccctacct attattccgt gtcacaacaa ctggtttata aagtgggtca
    16741 gtatcgccca cgtacgctgg gcaacctgac ggcggagcaa ctcaccgttg caccgggtca
    16801 tgtggtggtt aacgatctcc agaccctgaa agaaacaaaa ttcccggaat taagctggaa
    16861 ggtagacgac aaaaaaggct ctgcggaatt aatggaagat gtcatcgaag gaaaactcga
    16921 ttacaccatt gctgattctg tcgccatcag cctgtttcag cgcgttcacc cggagctcgc
    16981 cgtagcgctc gatatcaccg atgaacaacc ggtgacttgg tttagcccgt tagatggcga
    17041 taataccctt tccgccgccc tgctcgactt cttcaacgaa atgaatgaag acggtacgct
    17101 ggcacgcatt gaagagaaat acctggggca tggcgatgat tttgattacg tcgatacgcg
    17161 cacattttta cgcgccgtcg atgcggtact gccgcagtta aagcccctgt ttgagaaata
    17221 cgccgaagaa attgactggc gtttgctggc cgctattgct tatcaggaat cgcactggga
    17281 tgcacaggcc acttcaccga cgggtgtgcg cggcatgatg atgttaacca aaaataccgc
    17341 gcaaagcctc ggcattacgg atcgtaccga tgccgaacag agcatcagcg gtggcgtgcg
    17401 ttatttgcag gatatgatga gtaaagtgcc ggaaagtgtg ccggagaacg aacggatctg
    17461 gtttgccctc gctgcgtaca atatgggcta tgcgcatatg ctggatgccc gcgccctgac
    17521 ggcaaaaacc aaagggaatc ctgacagttg ggctgacgta aaacagcgtc tgcctttact
    17581 tagccagaaa ccctattaca gcaagctgac ttacggctac gctcgtgggc atgaagccta
    17641 cgcttatgtc gaaaatattc gtaagtatca gattagcctg gtgggttatc tgcaagagaa
    17701 agagaagcag gctacagaag cggcgatgca actggcgcag gattatccgg cggtatcgcc
    17761 tacggagttg ggcaaagaga aatttccttt tctctcgttt ctttcccagt cgtcatcaaa
    17821 ctatttgacc cattctccct ctctgctgtt ttccaggaaa gggagtgaag agaaacaaaa
    17881 ttaatccgtc gaggattgcg cttttttctg cgctttaatt tcctggcggc gcatgcgaaa
    17941 gaagtcactg agcaacgccg cgcactcatc cgccagtatt ccttccgtaa tttccactcg
    18001 gtgattcata cccggatgat gcagcacatc cattaaagat cccgcagcgc cagttttcgc
    18061 gtcacgcgca ccaaagacca cgcgaccaat gcgactgtgg atcatcgctc cggcacacat
    18121 tacacatggt tcaagcgtga catacaacgt ggcgtcgatc agacgataat tttgcatcac
    18181 cagaccaccc tgccgcaggg ccatgatttc tgcatgtgcg gtgggatcat ggcgaccaat
    18241 cgggcggttc cagccttcgc cgattacccg attgttatgc actaataccg cgccgaccgg
    18301 cacttcccgc tcatcccagg cacgtttcgc cagcgtcagc gcgtgacgca tccagtattc
    18361 gtggctaaat tcgacttcag acaaaaagaa aactccggtt ataaaagcgc ggcgcattat
    18421 acacggacgc tatgctttac tctattccag ttgctggagt tcaccgcgcg gggttactcg
    18481 ccaacgatgc tgacagaaat aaagcagcgg attgtcctga ttactgtcgc tatagccact
    18541 gtacagccgc agcggcgtgc cgattttgcg ctccagttgt gcgacctttt catgtcccag
    18601 acaacgcatc gtcaataccc aaccaccata gccacgctga atttggctgg cgataagatt
    18661 aacccgcggc agccagggcg tatcgaaata aaccgcttca accagcggct gcggagagcc
    18721 ggtaatcaac cagatatcag catcggaact taacaggtag gtggttaatc gctcctgaac
    18781 cagcggaaag gcggtaacat tgtcgcgaaa ccagcgcacg aaatcggcct gcaacgtctg
    18841 taaacgtgct tcgctgtgac caaaagtgca cccccacaga agcagactca tcggccagcg
    18901 tgccgcacga ccttttatca ataacgcaat ggctataatc ggtaacaacg gcaggacaag
    18961 taacgcattc agcggttggc gacgcagtaa atagcgcaga aaactgccga acatatcctg
    19021 ctgatgcaat gttccatcta agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc
    19081 atactcctct ggactgaaac atcctgatta attttcgcta ctacatagcc taacagatag
    19141 atcatcactt ttccggcaac aatcgaccgc atcatggcta actggaattt ttaattcatg
    19201 gcaattagcg gcaatggaat ataaaattca ctcgcgtgtg tctcatattg cctgttgtcg
    19261 ccactatttc atcgcgagga aagatatgaa cggcttactt cgtatccgtc agcgttacca
    19321 ggggcttgcc cagagtgata aaaaactggc ggattatctg ctgctacaac ctgatacggc
    19381 gcgccattta agctctcagc aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt
    19441 gaagttcgcg caaaaactcg gctataaagg ttttccggcg cttaagttgg cgttgagtga
    19501 agcgctggca agccagccgg aatcgccctc cgtgcccatt cataaccaaa tccgcggtga
    19561 tgatccgtta cggctggtcg gcgaaaaact gattaaagaa aatactgccg ctatgtacgc
    19621 aacgctaaac gttaatagtg aagagaaact gcatgaatgc gtaacaatgt tgcgctctgc
    19681 gcggcggata attctgaccg gtattggcgc ttcgggtctg gtggtgcaaa actttgcctg
    19741 gaagctgatg aagattggct tcaatgctgc cgcactgcgc gatatgcatg cgctactcgc
    19801 aacagtacag gcgtcgtccc ctgacgatct gttattagcc atttcctaca ccggtgtacg
    19861 acgcgagtta aacctggcgg cagatgagat gctgcgagtg ggcggaaaag tgctggcgat
    19921 taccggcttt actccgaatg ccctgcaaca gcgtgcttct cattgcctgt ataccattgc
    19981 cgaagaacaa gcgacaaaca gtgcttcaat ctctgcttgt cacgctcagg gaatgttaac
    20041 ggatttgctg ttcattgcgc tgattcagca ggatctggaa ctggcaccag aacgtattcg
    20101 tcatagtgaa gcgctggtga aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc
    20161 ccggtttgtg ttgttttgag agtttcctta tggcgctgtt aatcactaaa aaatgcatca
    20221 attgtgatat gtgtgaaatc aaataaaaac acatttattt tcatagtgat acagcaatgt
    20281 gttattgaat gtgttgttgg atttatggtg caaaaaaatt ttgcataagt tcagcaacac
    20341 aaagaacacc actttcttta acgtcctccg cgccctaaag gacggggctt tacggcgcaa
    20401 aaggcctgtg acatacaaca ttaacttttc agtcaaccgt agcacgtcct tgtatacgcc
    20461 gtgtctgcgg ttttatataa ccctggctgg aaacctctta tacaaagtcg attcgccaac
    20521 atcatagatg atcgccacct tctggcgaga aactcctgat gcaattagtc gcccggcctg
    20581 cgcccattat tctggtgaaa gtttgggacg gcgaccgcca attcgtccct gtgcgcgagc
    20641 agcttccagt cctgcttttg ttcgttcaac aatcagttca cgttccattt cagccagggc
    20701 acccatcaca tggaagaaaa agcgtcccat tggtgtgctg gtatcaattg aatcagtcag
    20761 actacgaaag ttgatgcctc gttcgcgcaa ctcctccacc agcacgacaa gatgccgcat
    20821 actgcgcccc agccgatcca gcttccagac aaccagtgtg tcgcctgccg ataatgtcct
    20881 gagcagtttt ttcagccccg gtctttcgga ctttgtacca cttatcttgt cttcaaaaat
    20941 cagctcgcat cctgcacagt tcaacgcatt acgttgtaga tcggtattct ggtcatttgt
    21001 tgatacgcgt acatagccaa taagcatggt aaatccc
//
LOCUS       DADWTH010000064        20686 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.064, whole genome shotgun sequence.
ACCESSION   DADWTH010000064 DADWTH010000000
VERSION     DADWTH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.064"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNY02_002542"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNY02_002542"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBB9434044.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JNY02_002543"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JNY02_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBB9434045.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JNY02_002544"
     CDS             complement(2649..3200)
                     /locus_tag="JNY02_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBB9434046.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JNY02_002545"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JNY02_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBB9434047.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JNY02_002546"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JNY02_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBB9434048.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNY02_002547"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNY02_002547"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBB9434049.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNY02_002548"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNY02_002548"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBB9434050.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNY02_002549"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNY02_002549"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBB9434051.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLTKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKFIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNY02_002550"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNY02_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNY02_002551"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNY02_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBB9434052.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JNY02_002552"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JNY02_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBB9434053.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JNY02_002553"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JNY02_002553"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBB9434054.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNY02_002554"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNY02_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBB9434055.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNY02_002555"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNY02_002555"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBB9434056.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNY02_002556"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNY02_002556"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBB9434057.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSQTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNY02_002557"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNY02_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBB9434058.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="JNY02_002558"
     CDS             complement(18174..18569)
                     /locus_tag="JNY02_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBB9434059.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTEVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNY02_002559"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNY02_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB9434060.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="JNY02_002560"
     CDS             19485..20078
                     /locus_tag="JNY02_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB9434061.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="JNY02_002561"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="JNY02_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBB9434062.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4916 a         5208 c         5489 g         5073 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc ggatggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcactgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaaa catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gatgaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agtcagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt caggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggtggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtctgg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggaaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 acctccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaagcattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgc
//
LOCUS       DADWTH010000065        20668 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.065, whole genome shotgun sequence.
ACCESSION   DADWTH010000065 DADWTH010000000
VERSION     DADWTH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.065"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNY02_002562"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNY02_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBB9434063.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNY02_002563"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNY02_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBB9434064.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNY02_002564"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNY02_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBB9434065.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIHEQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNY02_002565"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNY02_002565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBB9434066.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JNY02_002566"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JNY02_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBB9434067.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JNY02_002567"
     CDS             complement(8217..9110)
                     /locus_tag="JNY02_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBB9434068.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNY02_002568"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNY02_002568"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBB9434069.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="JNY02_002569"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="JNY02_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBB9434070.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JNY02_002570"
     CDS             complement(10241..11473)
                     /locus_tag="JNY02_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBB9434071.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNY02_002571"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNY02_002571"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBB9434072.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVVMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNY02_002572"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNY02_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBB9434073.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16497)
                     /locus_tag="JNY02_002573"
                     /pseudo
     CDS             complement(14278..16497)
                     /locus_tag="JNY02_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNY02_002574"
     CDS             complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNY02_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBB9434074.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNY02_002575"
     CDS             complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNY02_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBB9434075.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNY02_002576"
     CDS             complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNY02_002576"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBB9434076.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19788..20552)
                     /gene="ycaL"
                     /locus_tag="JNY02_002577"
     CDS             complement(19788..20552)
                     /gene="ycaL"
                     /locus_tag="JNY02_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBB9434077.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4968 a         5712 c         5017 g         4971 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcaaacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgt ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc atattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaaacct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcaccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagaccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcaatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggctatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa
    15121 cgttcgccag gcgtttaatc accataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag
    15241 gattaacggc ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa
    15301 tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa
    15361 tatgacgatt tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga
    15421 aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca
    15481 aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac
    15541 aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg
    15601 tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg
    15661 aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg
    15721 tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc
    15781 agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc
    15841 agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa
    15901 accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata
    15961 ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag
    16021 cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc
    16081 atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg
    16141 aagttattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata
    16201 agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc
    16261 ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc
    16321 ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc
    16381 cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa
    16441 aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca
    16501 cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg
    16561 cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg
    16621 cgaggcagct tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct
    16681 cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta
    16741 ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga
    16801 ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca
    16861 atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct
    16921 tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac
    16981 ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga
    17041 ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca
    17101 agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta
    17161 gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta
    17221 acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt
    17281 gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga
    17341 accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca
    17401 acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt
    17461 acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt
    17521 ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt
    17581 tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca
    17641 gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg
    17701 atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc
    17761 cacgggttag ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata
    17821 tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt
    17881 ttgttggtcc aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca
    17941 acgaagcagc cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga
    18001 taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc cagggataca
    18061 cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg
    18121 atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca
    18181 acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact
    18241 tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg
    18301 ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta
    18361 aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga
    18421 gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg
    18481 ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag
    18541 gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact
    18601 gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt
    18661 ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg
    18721 tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct
    18781 tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat
    18841 atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc
    18901 attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt
    18961 attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag
    19021 catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt
    19081 tgatctcggc caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca
    19141 tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag
    19201 ggaataccac cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg
    19261 cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg
    19321 cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt
    19381 ccagattgcc attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg
    19441 catcttccga cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa
    19501 ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc
    19561 ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta
    19621 tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca
    19681 aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg
    19741 caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag
    19801 gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg
    19861 gactgggtgc gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg
    19921 gtgctgatgc cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta
    19981 tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa
    20041 agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa
    20101 gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga
    20161 acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag
    20221 ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc
    20281 gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg
    20341 ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc
    20401 gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata
    20461 gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc
    20521 gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat
    20581 aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc
    20641 acaaggtccg aaaaaaaacg ccgacaat
//
LOCUS       DADWTH010000066        20607 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.066, whole genome shotgun sequence.
ACCESSION   DADWTH010000066 DADWTH010000000
VERSION     DADWTH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.066"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="JNY02_002578"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="JNY02_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBB9434078.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="JNY02_002579"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="JNY02_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBB9434079.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="JNY02_002580"
     CDS             complement(977..1267)
                     /locus_tag="JNY02_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBB9434080.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="JNY02_002581"
     CDS             1701..2411
                     /locus_tag="JNY02_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBB9434081.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JNY02_002582"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JNY02_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBB9434082.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="JNY02_002583"
     CDS             complement(3539..4198)
                     /locus_tag="JNY02_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBB9434083.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /gene="bisC"
                     /locus_tag="JNY02_002584"
     CDS             4351..6684
                     /gene="bisC"
                     /locus_tag="JNY02_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBB9434084.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="JNY02_002585"
     CDS             complement(6653..7093)
                     /locus_tag="JNY02_002585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB9434085.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JNY02_002586"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JNY02_002586"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBB9434086.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="JNY02_002587"
     CDS             7811..8509
                     /locus_tag="JNY02_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBB9434087.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDSTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="JNY02_002588"
     CDS             8738..9946
                     /locus_tag="JNY02_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9434088.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="JNY02_002589"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="JNY02_002589"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBB9434089.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="JNY02_002590"
     tRNA            12053..12129
                     /locus_tag="JNY02_002590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="JNY02_002591"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="JNY02_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBB9434090.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="JNY02_002592"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="JNY02_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBB9434091.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="JNY02_002593"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="JNY02_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBB9434092.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="JNY02_002594"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="JNY02_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBB9434093.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="JNY02_002595"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="JNY02_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBB9434094.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="JNY02_002596"
     CDS             complement(18744..20015)
                     /locus_tag="JNY02_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBB9434095.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20491..20598
                     /locus_tag="JNY02_002597"
     CDS             20491..20598
                     /locus_tag="JNY02_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBB9434096.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4929 a         5097 c         5423 g         5158 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atagcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt ttatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgctccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cttggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcggcaagcc ggaagggttc tggtgaggcg caatgttgcg
    20341 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    20401 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
    20461 aaacgttgtc attacaacac aggcggctat atgacgctcg cagagctggg catggccttc
    20521 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
    20581 ctgaacaagc ggaagtagcg tgtcatg
//
LOCUS       DADWTH010000067        20549 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.067, whole genome shotgun sequence.
ACCESSION   DADWTH010000067 DADWTH010000000
VERSION     DADWTH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.067"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JNY02_002598"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JNY02_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBB9434097.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JNY02_002599"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JNY02_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBB9434098.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JNY02_002600"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JNY02_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBB9434099.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNY02_002601"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNY02_002601"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBB9434100.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNY02_002602"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNY02_002602"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBB9434101.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JNY02_002603"
     CDS             4449..4814
                     /locus_tag="JNY02_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBB9434102.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JNY02_002604"
     CDS             4964..5482
                     /locus_tag="JNY02_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBB9434103.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JNY02_002605"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JNY02_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JNY02_002606"
     CDS             6713..7195
                     /locus_tag="JNY02_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBB9434104.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JNY02_002607"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JNY02_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBB9434105.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JNY02_002608"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JNY02_002608"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBB9434106.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JNY02_002609"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JNY02_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBB9434107.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JNY02_002610"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JNY02_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBB9434108.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JNY02_002611"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JNY02_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBB9434109.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="JNY02_002612"
     CDS             10939..11730
                     /locus_tag="JNY02_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBB9434110.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JNY02_002613"
     CDS             11751..13037
                     /locus_tag="JNY02_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBB9434111.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JNY02_002614"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JNY02_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBB9434112.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JNY02_002615"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JNY02_002615"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBB9434113.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JNY02_002616"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JNY02_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBB9434114.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JNY02_002617"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JNY02_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBB9434115.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JNY02_002618"
     CDS             complement(16985..17275)
                     /locus_tag="JNY02_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBB9434116.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="JNY02_002619"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="JNY02_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBB9434117.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JNY02_002620"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JNY02_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBB9434118.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JNY02_002621"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JNY02_002621"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JNY02_002622"
     CDS             19137..20378
                     /locus_tag="JNY02_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBB9434119.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAAARGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5364 c         4827 g         5056 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgt gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcggac agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgttgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaattagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcta
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttagacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgcat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatctc tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgccagtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggctcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg caggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ctgatttag
//
LOCUS       DADWTH010000068        19914 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.068, whole genome shotgun sequence.
ACCESSION   DADWTH010000068 DADWTH010000000
VERSION     DADWTH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.068"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..744)
                     /locus_tag="JNY02_002623"
     CDS             complement(<1..744)
                     /locus_tag="JNY02_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9434120.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            838..1035
                     /locus_tag="JNY02_002624"
     CDS             838..1035
                     /locus_tag="JNY02_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB9434121.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGTHIENDDRIN"
     gene            1129..1317
                     /locus_tag="JNY02_002625"
     CDS             1129..1317
                     /locus_tag="JNY02_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB9434122.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1268..1963
                     /locus_tag="JNY02_002626"
     CDS             1268..1963
                     /locus_tag="JNY02_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HBB9434123.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(2075..2422)
                     /locus_tag="JNY02_002627"
                     /pseudo
     CDS             complement(2075..2422)
                     /locus_tag="JNY02_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="JNY02_002628"
                     /pseudo
     CDS             complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="JNY02_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4429..5487
                     /gene="citC"
                     /locus_tag="JNY02_002629"
     CDS             4429..5487
                     /gene="citC"
                     /locus_tag="JNY02_002629"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBB9434124.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5502..5798
                     /gene="citD"
                     /locus_tag="JNY02_002630"
     CDS             5502..5798
                     /gene="citD"
                     /locus_tag="JNY02_002630"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBB9434125.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5795..6703
                     /gene="citE"
                     /locus_tag="JNY02_002631"
     CDS             5795..6703
                     /gene="citE"
                     /locus_tag="JNY02_002631"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBB9434126.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6714..8246
                     /gene="citF"
                     /locus_tag="JNY02_002632"
     CDS             6714..8246
                     /gene="citF"
                     /locus_tag="JNY02_002632"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBB9434127.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8250..8801
                     /gene="citX"
                     /locus_tag="JNY02_002633"
     CDS             8250..8801
                     /gene="citX"
                     /locus_tag="JNY02_002633"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBB9434128.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8776..9654
                     /gene="citG"
                     /locus_tag="JNY02_002634"
     CDS             8776..9654
                     /gene="citG"
                     /locus_tag="JNY02_002634"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBB9434129.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9705..11168
                     /gene="citT"
                     /locus_tag="JNY02_002635"
     CDS             9705..11168
                     /gene="citT"
                     /locus_tag="JNY02_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBB9434130.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11282..12088
                     /gene="rna"
                     /locus_tag="JNY02_002636"
     CDS             11282..12088
                     /gene="rna"
                     /locus_tag="JNY02_002636"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBB9434131.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12317..12727
                     /gene="rnk"
                     /locus_tag="JNY02_002637"
     CDS             12317..12727
                     /gene="rnk"
                     /locus_tag="JNY02_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBB9434132.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12958..14195)
                     /locus_tag="JNY02_002638"
                     /pseudo
     CDS             complement(12958..14195)
                     /locus_tag="JNY02_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14416..14844
                     /gene="uspG"
                     /locus_tag="JNY02_002639"
     CDS             14416..14844
                     /gene="uspG"
                     /locus_tag="JNY02_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBB9434133.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISPHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="JNY02_002640"
     CDS             complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="JNY02_002640"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBB9434134.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="JNY02_002641"
     CDS             complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="JNY02_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBB9434135.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17710..18456
                     /gene="dsbG"
                     /locus_tag="JNY02_002642"
     CDS             17710..18456
                     /gene="dsbG"
                     /locus_tag="JNY02_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBB9434136.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18666..19568
                     /locus_tag="JNY02_002643"
     CDS             18666..19568
                     /locus_tag="JNY02_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9434137.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19715..19914
                     /locus_tag="JNY02_002644"
                     /pseudo
     CDS             19715..19914
                     /locus_tag="JNY02_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5017 a         5128 c         4757 g         5012 t
ORIGIN      
        1 aacttgcgaa ccgcaaagcc agttcccgtt attatccatg tacgcctgac catcggtgcc
       61 attggctgtc cttgagttat taatcatgta gatgccaaat tgcctattcc ccagcccacc
      121 aattacgaat ttacggtcgg tatggtcctg gcgaagcaat gcctgcgcac catcgttgga
      181 taccgcatta cggcccataa taacgttctg gtcacgcata tgaatccaca tgcctgtact
      241 gctgttaatt aaaaaacggt ttgtaaatat atctcctgta acaaccagac catgccccat
      301 gcttatccgg ccagttctga gattaagcgt aaaggggcgt agtggcccta tattaccatt
      361 ttccccttca ttctctcgtg tagggatgat atgcaggcat tcttcagaac gacgaaaaat
      421 ggcaccaaaa gatgaattaa atattctcag tgcattgacc gtcgatattt ttacttcact
      481 gctgaaaagg gctttaacaa gaacatacag agcatcccat ttaagattca tcaggtcttt
      541 aacccggccg ccagtgaggt ggtgtctcca tttgaaatac tcattgccgt tgtcgcctgt
      601 ttcaaaccac atgtatgaat cagtatcgct gtcggcatca tttttaaatc caatctttgc
      661 ccagtcagta ttccgaatcc aggcaaggat tgagtcgttt tcaaaagtaa gtccaccgga
      721 caacgtatcg ccattttttt gcacggcgtt accagccttg tttaccgttt cctgtaaacc
      781 gaggggccgt ttctggcctg catggcatga tttccgcttt taaacgggga attcagcgtg
      841 ctgattggct atgtaagggt atcaacaaat gaccagaata cagacctgca acgaaacgct
      901 cttgtttgtg caggatgtga acaaatattt gaagataaat taagcggaac aaggacagac
      961 cgaccgggat taaaacgtgc tttaaagcgc cttcaaaaag gaacgcatat agaaaacgac
     1021 gatcgaatca attaaatcga tctatattac cgatcgatta tgtcccgata accacacccg
     1081 actgatgaga ttttttaaga taacggcgaa ttgatgcaag gaggtgaaat gaaatcaatt
     1141 cgctgtaaga actgcaacaa actgttattt aaagcggatt cctttgatca cattgaaatc
     1201 aggtgtccgc gttgcaaacg tcacatcata atgctgaatg cctgcgagca tcccacggag
     1261 aaacattgtg ggaaaagaga agaaatcacg cattctgacg aaaccgtgcg ttattgagta
     1321 tgaaggccag attgttggct atggttcaaa ggagctgcgc gttgaaacca tatcctgctg
     1381 gctggcccgc acaatcattc agacaaagca ctattcccgc cgttttgtga ataactctta
     1441 ccttcacctg ggagtattca gcgggcgtga tctggttggc gttctccagt ggggatatgc
     1501 ccttaacccc aactcaggtc gtcgtgtcgt gcttgaaacg gataaccggg gctatatgga
     1561 gttgaatcgc atgtggctac acgacgacat gccccgcaac tctgaatcac gggccatcag
     1621 ctacgcgctg aaagttatca gattactgta tccgtcagtg gagtgggttc agtcctttgc
     1681 tgacgaacgc tgcggactcg caggcgttgt gtatcaggcg tcgaattttg attttattgg
     1741 cagccatgaa agcacgttct acgagctgga tggtgaatgg tatcacgaga taacgatgaa
     1801 cgcgattaag cgcggagggc aacgaggcga gtatttgcga gctaataagg agcgtgccgt
     1861 ggtgcacaaa tttaatcagt atcgctacat cagattcctg aacaagcgag caaggaagcg
     1921 gctaaatacc aaactattca aggttcagcc ataccctaag tgatccccat gtaatgaata
     1981 aaaagcggta attaatactg ctgtttcatt tgaagcgcga aagctaaagt tttcgcattt
     2041 atcgtgaaac gctttcgcgt ttttcgtgcg ccgcttcacc cagccgttca taggcaattg
     2101 gcttaatgag ataatcaaat acaccacaac gtacggcttc agacaccgtt tccatatcac
     2161 tggctgcagt ggtaaacacc acgtcgccgg gataatgcgc ctgcaccagt tcatgcagta
     2221 aattaatccc tctaccgtca ggaagatagt tatcgagcaa gattagcccc ggcttaaaac
     2281 gctcgatcat cattcgggcc tgcgccaggt ttcccgccag taatatctga ctgaatccgg
     2341 gaatgtgacg aatatattcc gcatgcatct ctgccagcgg cgtttcgtcc tcaacgatca
     2401 atagggttaa tggagctgtc attaggtttc actttcggaa tatagattga aaataaggta
     2461 ccgcagggat cattatcttc gagagtgata acaccaccgc agcgcgttac gtagctggca
     2521 atcaagtaca acccaatgcc atgttcaccg ggctcgtcag cacgcgtact gacccctgct
     2581 caaatatttt gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg acttcaatca
     2641 ccacatcatc gccttcatcg ctgaggaata attcaacgct cttgtttcct tcatcgctac
     2701 gcaggctggc ttcgaaggcg ttatcaagta aattgccaac aatggcggca aactcggtgc
     2761 tatccagtcc tggcggcagt tgcgaaagct ggctaccggg gacgatgatc atttttagcc
     2821 ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc cgccacctgg cgatcggcaa
     2881 acgcctcgcg caggctgtca ataagctgtt gctgggcctg agactccccc tgcaccatcg
     2941 ccagcacgcg atcatactct ttcatctgca acagaccatt gagagtcgac atccaattga
     3001 gatgctcgtg tcgcaatgta cgaaggctct caacgtattg ttttatttgc gtcagttgcg
     3061 cattgagggt ggaaatttcg tctttactgc gaaagctgat aatggctccc agcaaatcat
     3121 cacctgaacg aatagcttcc cgattggcaa taacgctcag accgttaaag ttagccacca
     3181 catcctgacg tttttcatca atctgttcgg taaagaaatc ggcagacctg accacttcag
     3241 caatgggttt acccaaccat tgccgtccag gggaactcag ccccagcatc tttcttgcgt
     3301 tacgattgat ggcggtaatg taaccgtgcg gatccaccgc aatcagccct tcataaaccg
     3361 aactaaacag cgcctcttgc tgacggacca cccgtgcgat ttgctttggc tccatgccca
     3421 tcatctgccg acggatatgc gcggccagga accacgacag caacatcaga atccctaaca
     3481 gcacgacaaa cacacctgcc atcggtaata aaaactcagc ccgccagcta tcgattttac
     3541 tcaccaggta gccaatcgac accacgccga tgacttttcc atcgtcatca aagattggcg
     3601 ttttggcgcg catcgccata cccattgacc ctttcccggt aatgaagtag ctctcccctt
     3661 tctccagcgc gccctgtttg gtgaactgca taggataacc aattttctcc ggattaggat
     3721 ggtaaaggcg gatcgagtgc cggtcgccaa tcaccacata atcaaaatcg gtatctcttt
     3781 gtaatttgtt agcgatggtc gccagccgtt tgtagtcacg cgttttcacc gcagagatga
     3841 cactgtcatt ggaggcaata attttcgcct gattcattgc catgtcgcgt acatgaagcg
     3901 ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag ggctgcaatg acaaatattg
     3961 agaacaccag aatcagcaaa aagatacgca gcggaaatgt cagtctttgg aaaaatgcaa
     4021 actgtttatt ctcgttaagc tgcaacattg ttttccttgc gggttgtccc gagcctcttt
     4081 gacggatgta attaaaatgt tgaatcaaaa tacatagata ttaattatga tcgtggcaaa
     4141 ttttattcat taaatcaatt aaatcactta aacctgcgag tcatttcgta attacattaa
     4201 ttaaaaaact taaaaccatt aaacaaataa aaaaaccact aaccctatgt gaaataaatc
     4261 aaaatttcac gccgaaatac tccttaggat gtatagcgaa aggagaaaaa gatatacctc
     4321 gatcacaccc ctttctccca agtgaaaata aaaggttatc agtttgcaac attgaacaac
     4381 attcgttgca aatcgataac aacatgcacc ttcaggatac tatttattat gttcgtcaat
     4441 gatattttca cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc
     4501 ctgcatgaaa atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc
     4561 gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc
     4621 agtgaatccg ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc
     4681 gcctatgagc ggcacagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg
     4741 ttccgccagt gcggtttttc cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa
     4801 aacagcgcca cgcgactgaa acgctatgcc gaatcgctga aaaaattgcg tcatccaggg
     4861 aacaagattg gctgcattgt gatgaacgcc aatcccttta cgaatggtca ccgttatctg
     4921 attcaacaag ctgcggcaca gtgcgactgg tttcatctgt ttttagtcaa agaagattct
     4981 tcacgcttcc cctatgaaga ccggctggat ctggtgttaa aaggcaccgc cgatattcca
     5041 cgcctgactg tgcatcgcgg ctcagaatac atcatctccc gcgctacgtt cccttgctac
     5101 ttcattaaag aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc
     5161 cgtcagtacc tcgctcccgc gcttggtgta actcaccgct ttgtcggtac tgaacccttt
     5221 tgtcgcgtta ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc
     5281 tccgcagcgc ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata
     5341 tccgcttccc gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg
     5401 gtccctgcag tcacgctgca ttatttgcag aacctgcttg aacactcccg ccaggacgcg
     5461 gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg
     5521 ccgttgcagg cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc
     5581 aggatatcga cctgcaaatc aatagcagcg ttgagaaaca gtttggcgat gcaattcgca
     5641 ccaccattct ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg
     5701 acaaaggcgc actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca
     5761 gcggtatccc ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg
     5821 taaaactcgc acccgccgca gcatgttgtt tgtgcctggt gccaatgccg cgatggtcag
     5881 caactccttc atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgtggcatt
     5941 acgtgaaaaa gacaccgccc gccgcatggt ttatcacgcg ctgcaacatc cgctgtatcg
     6001 cgatattgaa accattgtgc gtgtcaacgc gctggattcc gaatggggtg ttaacgacct
     6061 ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca
     6121 ggatgttctg gatattgaaa aagagatcct gcgtatagaa aaagcctgtg gtcgtgaacc
     6181 cggcagcacc ggcctgctgg cggcgattga atctccgctg ggcattaccc gcgcagtgga
     6241 aatcgctcac gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg
     6301 caacctgcgt acagaacgct ccccggaagg aactgaactg ctgttcgcac gctgttccat
     6361 tttgcaggcc gcgcgctctg cgggtattca ggcgttcgat accgtctatt ccgacgctaa
     6421 caacgaagcc ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa
     6481 atcgctgatc aacccgcgtc agattgatct gctgcacaac ctctacgcac cgacccagaa
     6541 agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct
     6601 cggcgtggtt tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct
     6661 ggtgctctcc cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc
     6721 agaaaattga acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcactgaat
     6781 gcgatcttgc cgctttccag aactcaccaa agcaaaccta ccaggctgaa aaagcgcgcg
     6841 atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg ctcaggttta caggacggca
     6901 tgacggtttc cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg
     6961 acgtcatcgc gaagatgggc tttaaaaacc tgaccctggc gtccagctcc ctgagtgatt
     7021 gccatgcgcc gctggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctctg
     7081 gcctacgcgg tccactggcg gaagagatct cccgtggtct gctggcggaa ccggtgcaga
     7141 tccactctca cggcggtcgt gtgcatctgg tacagagcgg cgaactgaat atcgacgtgg
     7201 ctttcctcgg cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac accggtaaag
     7261 cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga
     7321 tgcttaccga agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg
     7381 ttgatttgat cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtacta
     7441 cccgtatgac cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg
     7501 tcaactctgg ctacttcaaa gaaggtttct ccatgcaaac cggcaccggc ggcgcttcgc
     7561 tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg
     7621 ccctcggcgg tattaccgcg acgatggttg atctgcacga aaaaggtctg atccgcaaac
     7681 tgctggatgt gcagagcttt gacagccatg ctgcgcaatc gctggcccgt aaccccaatc
     7741 acatcgaaat cagcgccaac cagtacgcta actggggttc gaaaggcgca tcggttgatc
     7801 gtctcgacgt ggtggtactg agcgcgctgg aaattgacag gcagttcaac gttaacgtgc
     7861 tgaccggttc tgacggcgta ctgcgtggcg cttccggtgg tcactgcgat accgcgattg
     7921 cctctgcgct ttccatcatc gtcgcgccac tggtacgcgg tcgtattccg actctggtgg
     7981 ataacgtgct gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg
     8041 gtatcgcagt taacccggca cgtccggaac tagcagaacg tctgcaggaa gcgggcatta
     8101 aagtggtttc cattgagtgg ctgcgcgaac gtgcgcgtct gctgaccggt gaaccacagc
     8161 cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg
     8221 atgttgtgca tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc
     8281 atgcggtgtc aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg
     8341 cctggctcaa gcaccatcct gttccactgg tctcctttac cgtggttgcg cctgggccga
     8401 ttaaagacag cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct
     8461 tagccacaaa gcagggctgg caaattcagg agcaggctgc actggtttcc gccagcgggc
     8521 cggagggcat gttgagcatt gccgccccgg ctcgcgatct caagctcgcc accattgagc
     8581 ttgaacatag tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg
     8641 aaattctctc ccgccgcgac tattcactgc cgcctcgccg ctgcctgttg tgcgagcaaa
     8701 gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg
     8761 aggcactgct gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg
     8821 tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaatctg
     8881 tcaccgaaac caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg
     8941 gaagatttcc accgcagcgc gctggcgatt cagggctggt taccccgttt cattgaattt
     9001 ggtgcctgta gtgcagaaat ggcaccggaa gcggtactcc acggattacg cccaattggt
     9061 atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggcagc
     9121 attttttctt tagggctgct atgtgcggca attggccgtt tgcttcagct caaccagccg
     9181 gttacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggcct gaccgatcgc
     9241 gaactgcgta ccaataattc acgactgacg gcaggtcaac ggttgtacca gcagcttggc
     9301 cttaccggcg cacgcggtga agccgaagcg ggttatccac tggtgatcaa ccacgccttg
     9361 ccgcattacc tcactctgct ggatcagggg ttagatcctg aactggcatt gctcgatacc
     9421 ttgctcctac tgatggctac caacggcgat accaacgttg catcgcgcgg tggcgagggg
     9481 ggcctgcgct ggctacagcg cgaggcgcaa acattattgc aaaaaggggg cattcgaacc
     9541 cccgccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt
     9601 ccaggcggca gtgccgacct gctgatcctt acctggtttt tagcacagat ttaattattt
     9661 aagcacttga taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag
     9721 ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt cttatccctg
     9781 tccccgacgg tatgccgccg caggcatggc attacttcgc tgtgtttgtg gcaatgattg
     9841 tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg gttactattt
     9901 gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta
     9961 atgcgcaaaa acaggcgctg aaatggggcc tggctggttt ttccagcacc acggtatggc
    10021 tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca
    10081 ttgccctttt cctggtgaaa ttcatggaca aacgcacgct gacgttgggt tatgcgattg
    10141 tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta
    10201 cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa atcattcccg aacgatccgt
    10261 ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt
    10321 catccatgtt tgtcaccggt gcggcaccaa acatgctggg tctggagttc gtcagcaaaa
    10381 ttgccggtat ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggggtta
    10441 tcttgcttat cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca
    10501 gtgaagaagt ggcaacctgg gcgggtgatg aactaaaaac catgggtgcg ctgacacgca
    10561 gagagtggac gctgattggc cttgtattgc tcagcttagg tttgtgggta tttggcagtg
    10621 aagtcattaa tgctactgcg gttggtctgc tggcagtttc gctaatgctg gctctgcacg
    10681 ttgtgccgtg gaaagacatt acccgctata acagcgcatg gaacacgctg gtcaacctgg
    10741 caactctggt tgtgatggct aacggcctga ctcgttctgg ttttattgac tggttcgccg
    10801 gtaccatgag tacgcacctg gaaggattct caccaaacgc aacggtgatt gtactggttc
    10861 tggtgttcta ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc
    10921 tgccggttat tctggccgtc ggtaaaggta ttccgggcgt accaatggaa caactgtgta
    10981 tcctgctggt gctgtctatc ggtatcatgg gctgtctgac gccgtatgca accggtcctg
    11041 gggtgattat ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa
    11101 tcttcggggt gatttacatc tctatgttgc tgttggttgg ctggccgatt ctcgccatgt
    11161 ggaactaata cgttcgccaa acgctgaata aatcatcacg cccaccaggt aagcccacct
    11221 ggcgggcttt tttatgattt aatagatagt attcagtgta tgtatgagtt ccacacccat
    11281 tatgaaagca ttctgccgta acgccgcgtt gctcgcggtt tctctgcttc ccttatcttc
    11341 tgccaacgcc gtagcgttgc aggcaaaaca gtatggcgat tttgatcgct atgtcctggc
    11401 cctctcctgg caaaccggat tttgccagag tcaacacgat cgaaatcgta acgaacgaga
    11461 tgaatgtcgc ctgcaaaccg aaacgaccaa caaagctgat tttctgaccg tacatggtct
    11521 gtggccagga ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg
    11581 cttcggttgc gctactcgcc caatcccgaa tctaccagaa gcacgcgcca gccgaatgtg
    11641 ttcatcgccg gaaaccgggt tatcactgga aacggccgct aaactaagtg aagtcatgcc
    11701 aggagctggc ggacgttcct gcctggaacg ctacgaatat gccaaacacg gtgcctgctt
    11761 tggttttgat ccggacgcat acttcggtac gatggtacgc ctgaatcaag aaattaaaga
    11821 gagcgaagcc ggaaaattcc ttgcggataa ttacggtaaa acagtgagcc gccgtgactt
    11881 tgacgccgcc tttgccaaaa gctggggaaa agagaacgtg aaagcagtta agctaacgtg
    11941 ccagggtaac cctgcgtatt tgactgaaat tcagatctcg atcaaagctg acgccatcaa
    12001 cgctccgctt tctgcaaact cattcttgcc acaacctcac ccaggtaact gtggcaaaac
    12061 ctttgtgatt gataaagcgg gttattaatc ctctgattta caaaacacgc taattcgtct
    12121 tccataacca tgccgattaa gaattataca taatgcgctg cattgaggaa ttccctgaat
    12181 gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat catcatgtga gttaaaccct
    12241 cgccgcctga cggtgagggt tttcttttgg gtatgtttat ctgcgacact cgcagtaccg
    12301 acgacatgga gtaaaaatgt ccagaccaac tatcatcatt aacgacctgg atgccgaacg
    12361 catcgatatt ctgctggagc aacccgccta tgctggtttg ccaatcgccg acgcgttaaa
    12421 cgcagagttg gatcgcgccc aaatgtgttc gccagaagag atgccacacg acgtggtgac
    12481 aatgaacagc cgggttaaat tccgcaatct tagagatggc gaagtgcgtg tgcgcacgct
    12541 ggtgtatccg gcaaaaatga ccgatagcaa tactcagctt tccgttatgg ctccggtagg
    12601 tgccgcactg ctggggctgc gcgttggcga ttccattcac tgggaacttc cgggcggcgt
    12661 tgcaacccac cttgaagtgc tggaactcga ataccagcca gaagctgctg gcgactacct
    12721 gctttaatcc catctgcaca agcgataact cgctttgccc agaggatgca ctcgcatcct
    12781 ctttacgcaa aatcagcaat ttttcctggt tctcactgtt cacttttgct gcaaaattat
    12841 tccgtttcgt ttgtgggaat aaccatggca gaagcatttt acatcctgat aggatttctg
    12901 attatggcgg cgattatcgt catggctgtc ctttacctcg aaaaccactc ctgacgatca
    12961 tattgttccc cccggcatcg cattcaccag acctgaaacc gccttctgcg ccgcctctgc
    13021 gctttgtgca ccgggtacca gaatcacctt acggcactcc tcttcacgtt tttcgaaaat
    13081 ctcatatccc cgggcggcct cttcaaacgg catatagtgg gtaacaattt cttctggttt
    13141 cagtaatcct ttctcaatta acggtaataa ttctcccagc catgcgtgaa cgtgggtctg
    13201 tcccatttta aacgtcaacc ctttatcaaa ggcgtcgcca aacaggaaac cgtgaataaa
    13261 tccagcgtag acgcccggta cgctaacaat gccgccacgc ctgaccgccg caatacactg
    13321 gcgcaacgct ttaccgctgc tgccctccag tttcaggtta gtcagcaccg tttccgtggt
    13381 gctgcctttc gcttcaaaac cgacggcgtc tattactgca tccacgcccc ggtgacctgc
    13441 cgtttgttca ataattgact gtgccggatc gctatcttca tcgaaattaa tcgggatcgc
    13501 gccgtagcgg tcagcggcga aacgcaagcg gtagggatga tgatcaacaa caaaaatctg
    13561 ttccgcaccg agcaaccgtg cacaggcgat tgtcaacaat cccacaggac cagcaccata
    13621 gactgcaacg cttgaacctt gttggatctg cacatttttt gctgcctgcc atgccgttgg
    13681 cagaatatca gaaaggaaaa gggctttatc atctgaaagc aaaggcggta ctttaaacgg
    13741 ccccacattc cctttaggga cgcggacata ttccgcctgc ccgccaggaa cgccaccata
    13801 caggtgacta taaccaaaca atgccgcggg agcaggtatc tgttttttat tgagcgcagc
    13861 gcctttaccc gcattggtat tttcgcaggc ggcatattgc tgtaatcgac agaaaaaaca
    13921 gtcgccacaa gcaatgacga acggaattac cacccggtcg cctttttgca aattttttac
    13981 gtccttcccg gtttcaacga cttccccata aattcatgac caaaaatatc gccatgttta
    14041 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttaccgta
    14101 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
    14161 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa
    14221 agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg
    14281 gagaattcct ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag
    14341 aaccagacaa atatcaggga taaatattat aatcatggcg aatgatttgt tcatgattaa
    14401 cagggagaaa gggttatgta taaggcaatc attatgccag ttgatgtatt tgaaatggaa
    14461 ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat
    14521 ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg
    14581 cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc
    14641 cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat
    14701 gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttatcgg ttctcgcaac
    14761 ccatcgattt cgccccatct gttaggttct aacgcctcga gcgtgatccg ccacgccaat
    14821 ctgcctgtac tggtcgttcg ctaacagata aaagtattta tgccgccctg cctggaacat
    14881 cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca
    14941 attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc
    15001 actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt
    15061 cgtacagtca cccgctgcga acacgccttt gacgttggtt tcacatttcg catcaatgat
    15121 aatctcgccc atgcggttac gttcgactgc gccttccagc cagttggtgt tcgggagcag
    15181 accaatctgg acaaaaatac cggccagttc gatgttgtga atattgccgc tgacacgatc
    15241 tcgatattcc agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt
    15301 cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt
    15361 catttctggc gcaaattcca gcagcgttac gtgctcaacg atacccgcca ggtcgattgc
    15421 cgcttccacg ccggagttac cgccgccgat aaccgctacg cgtttgcctt taaacagcgg
    15481 gccgtcgcag tgcgggcagt aggtcacgcc tttagtgcga tactgatctt cgcccggaac
    15541 gttcatgttg cgccattttg cacccgtcgc cacgataatg ctgcgtgctt tcagtaccgc
    15601 gccagaagct gtttcaatct ggtgtagacc accttcaacc gctgccggga tcagtttgct
    15661 ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc
    15721 tgccaacttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc
    15781 gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga
    15841 gtaaattgct gccgctgcac ccgccgggcc ggaaccaacg attaatacgt cataagcatc
    15901 acgcttgttc agctcttctg ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat
    15961 ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac
    16021 gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg
    16081 cttgatgcgc gggttcaata cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt
    16141 atggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg
    16201 ctccagcaga gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac
    16261 cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggtccct ggttggaacc
    16321 tgggttggtg atcaggaaag acggcttacg cactggcaag ctgttatctt ctttaaaggt
    16381 gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc
    16441 gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt
    16501 gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat
    16561 gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg
    16621 ggtgccgcag cacccgaaga attaggtgaa tttccgaagt atttcgcgtc agagcaaggc
    16681 gactagcctg tgaatccccg ggagcttaca caagtaagtg accggggtga gcgggcgacg
    16741 ccaacgccgc tatggcgtga aagacgaagg aaatttagat tttaccaacc aggtccagag
    16801 acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag
    16861 aagctacgta ctgagctgct ttgattttac gcagcagatc agacgcgtca cggccaatgc
    16921 cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac
    16981 ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg
    17041 ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt
    17101 tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt
    17161 cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg
    17221 ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt
    17281 cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct
    17341 atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat
    17401 gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga
    17461 actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa
    17521 tctgaactac cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt gcccttacaa
    17581 atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag
    17641 ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag
    17701 aacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca
    17761 gaggaacttc cttccccagt aaaagcgatt gaaaaacagg gcattacaat catcaaaacg
    17821 ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc
    17881 atctacctga ctcccgatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt
    17941 gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg
    18001 tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt
    18061 gtctacgtct tcgccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc
    18121 ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca
    18181 gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa
    18241 caatatgaag cctctggttg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag
    18301 caaatgaaag tgttaagtga caatgagaaa ctgatggacg atctgggggc aaatgtcacg
    18361 ccggctatct attacatgag taaggaaaat acgctgcaac aggccgtggg gttgcccgat
    18421 cagaaaacgc ttaatatcat tatgggaaat aaataaaagg taattcatgg atggttgaac
    18481 tatatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg
    18541 ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat
    18601 acatacgcaa aaaacattta ttaagtgaat ataccatgga agaaaaatat aaaccggagt
    18661 agtgtatggc caatctctac gacttgaaaa agttcgactt aaatcttctt gtcatatttg
    18721 agtgtattta ccagcattta agcattagca aagctgctga atcactatat atcaccccat
    18781 cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc
    18841 gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga
    18901 aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa
    18961 actttattat ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct
    19021 gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga
    19081 atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga
    19141 tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta
    19201 ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca
    19261 cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt
    19321 ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg
    19381 cagttacaga tttactggga atagtcccct atgaacttta taactaccac cgtgactttc
    19441 ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct
    19501 ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga
    19561 gtttttgata tataactaca gacagctact ataactttca tctatttatt cacagctcga
    19621 atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac
    19681 tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc
    19741 actcaatatt ctggaagctg cacgagagcg tattacctag acactcaaca ccctaccccg
    19801 cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga
    19861 acttgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aggc
//
LOCUS       DADWTH010000069        19906 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.069, whole genome shotgun sequence.
ACCESSION   DADWTH010000069 DADWTH010000000
VERSION     DADWTH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.069"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..375
                     /locus_tag="JNY02_002645"
     CDS             <1..375
                     /locus_tag="JNY02_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HBB9434138.1"
                     /translation="GAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMS
                     ERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIG
                     FFGILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="JNY02_002646"
     CDS             complement(411..1367)
                     /locus_tag="JNY02_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBB9434139.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IITALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNY02_002647"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNY02_002647"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBB9434140.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQCEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="JNY02_002648"
     CDS             complement(2605..3366)
                     /locus_tag="JNY02_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBB9434141.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="JNY02_002649"
     CDS             3499..3909
                     /locus_tag="JNY02_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBB9434142.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="JNY02_002650"
     CDS             complement(3871..4977)
                     /locus_tag="JNY02_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9434143.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="JNY02_002651"
     CDS             complement(4988..6121)
                     /locus_tag="JNY02_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9434144.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="JNY02_002652"
     CDS             complement(6114..7850)
                     /locus_tag="JNY02_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB9434145.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8838)
                     /gene="hlyD"
                     /locus_tag="JNY02_002653"
     CDS             complement(7843..8838)
                     /gene="hlyD"
                     /locus_tag="JNY02_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBB9434146.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNY02_002654"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNY02_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBB9434147.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEETERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="JNY02_002655"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="JNY02_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNY02_002656"
                     /pseudo
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNY02_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="JNY02_002657"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="JNY02_002657"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBB9434148.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14883
                     /gene="ybiB"
                     /locus_tag="JNY02_002658"
     CDS             13915..14883
                     /gene="ybiB"
                     /locus_tag="JNY02_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBB9434149.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPL
                     LAILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPV
                     GAFCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFS
                     DIGGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETT
                     AQWTERCLAGSEAIPESLKIQMACCLVATGEVATISDGLARVNQAF"
     gene            15024..16109
                     /gene="hcxB"
                     /locus_tag="JNY02_002659"
     CDS             15024..16109
                     /gene="hcxB"
                     /locus_tag="JNY02_002659"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBB9434150.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16338..16598)
                     /locus_tag="JNY02_002660"
     CDS             complement(16338..16598)
                     /locus_tag="JNY02_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBB9434151.1"
                     /translation="MKTINTVVAAMALSTLSFAVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16890..17129)
                     /locus_tag="JNY02_002661"
     CDS             complement(16890..17129)
                     /locus_tag="JNY02_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HBB9434152.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNR"
     gene            complement(17203..17880)
                     /gene="ybiX"
                     /locus_tag="JNY02_002662"
     CDS             complement(17203..17880)
                     /gene="ybiX"
                     /locus_tag="JNY02_002662"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBB9434153.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17922..19906)
                     /locus_tag="JNY02_002663"
                     /pseudo
     CDS             complement(17922..19906)
                     /locus_tag="JNY02_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4618 a         5226 c         5378 g         4684 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggtgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gcactgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gtttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggctagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aaggggaagc agagatgctc
    14101 ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg
    14161 atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta
    14221 ttggcgattc tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat
    14281 ccaactcgcg tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac
    14341 ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg
    14401 ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt
    14461 gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc
    14521 agcgtttcgc atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg
    14581 tgggcgttac taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg
    14641 cagatcaatc tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct
    14701 ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt
    14761 tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg
    14821 gtggctacgg gtgaagtggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt
    14881 taatttttat ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa
    14941 attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca
    15001 cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc
    15061 acagttttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag
    15121 ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga
    15181 tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa
    15241 tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg
    15301 cggcacacga agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg
    15361 tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag
    15421 cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt
    15481 tccacggtcg cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag
    15541 ataatttccc gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg
    15601 tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc
    15661 cgacgaccaa tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg
    15721 aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg
    15781 gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga
    15841 ccactatcat catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag
    15901 cctttgccga gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg
    15961 gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg
    16021 gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc
    16081 aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa
    16141 caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg
    16201 gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta
    16261 ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc
    16321 cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg
    16381 gtggcggaag tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc
    16441 gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg
    16501 ttctgtgcct gggatgccgt taccggttcc gccgcgaaca cggcaaatga cagagttgaa
    16561 agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt
    16621 tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata
    16681 aaatcgaaga gaattgaccg ccttgttcaa ataaattgat tgatatctaa catacagata
    16741 aaccatttat ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt
    16801 taaaggcacg gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt
    16861 agttaacgta actggctgtc tttcgaacct cagcgattat atcctgtata cgctggtttt
    16921 tgtaaatctt tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga
    16981 cgggcttgcg ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga
    17041 atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg
    17101 gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt
    17161 taagtgtagc taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc
    17221 ccgcagcaga ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg
    17281 gcttttcagc gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc
    17341 gcggatcatc gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt
    17401 cacgcaatgc aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg
    17461 atgttgtcca aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct
    17521 taaaaacagc gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac
    17581 tgcgccatcc acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt
    17641 ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag
    17701 cacctcattt tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt
    17761 aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag
    17821 ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat
    17881 acaagttctc caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct
    17941 gtgagcaaga aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag
    18001 gcgacgtaat cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta
    18061 acccgatacc ccagtttggc atctgcgacc cagtaacctt ctgtaaatgc tggcgttccc
    18121 accgcgccgt ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag
    18181 atatcatcgg ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc
    18241 agcgatgagg aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg
    18301 gtatagccgc caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag
    18361 ccttcgacgc gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca
    18421 ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc
    18481 catttggtgc caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca
    18541 ctgttaccac tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa
    18601 acggcatagt taatatagac attgccgttt tccgtcaggt gatacagcgc cccggctttc
    18661 cagttcacca gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc
    18721 acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggtggt ggcactgtca
    18781 tattcagtat gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt
    18841 tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg
    18901 ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt
    18961 aacgtcaccg gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta
    19021 ctgacatcat gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg
    19081 agaattttat tactcacatc tttgatattc gccgtgcgtg actaggtcca gctattcaca
    19141 tcgctggtgg gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc
    19201 tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc
    19261 tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa
    19321 aagttatgag tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca
    19381 gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca
    19441 tgcagataat taagatacaa acgattcgct gtaccaaggc caaaagcgac agaaggggcg
    19501 acgccgtaac gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc
    19561 acattcaggc gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg
    19621 cggaaccagg cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc
    19681 ttgctgatca tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc
    19741 cctttaatca cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg
    19801 cgaataccat caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg
    19861 ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac ccacgc
//
LOCUS       DADWTH010000070        19779 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.070, whole genome shotgun sequence.
ACCESSION   DADWTH010000070 DADWTH010000000
VERSION     DADWTH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.070"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..441
                     /gene="feaR"
                     /locus_tag="JNY02_002664"
     CDS             <1..441
                     /gene="feaR"
                     /locus_tag="JNY02_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBB9434154.1"
                     /translation="QESMNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQ
                     KVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAI
                     RHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(613..942)
                     /locus_tag="JNY02_002665"
     CDS             complement(613..942)
                     /locus_tag="JNY02_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBB9434155.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(947..1132)
                     /locus_tag="JNY02_002666"
     CDS             complement(947..1132)
                     /locus_tag="JNY02_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBB9434156.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1129..3768)
                     /locus_tag="JNY02_002667"
     CDS             complement(1129..3768)
                     /locus_tag="JNY02_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBB9434157.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLDVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3976..4965
                     /gene="ldhA"
                     /locus_tag="JNY02_002668"
     CDS             3976..4965
                     /gene="ldhA"
                     /locus_tag="JNY02_002668"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBB9434158.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAVLELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5076..5412
                     /locus_tag="JNY02_002669"
                     /pseudo
     CDS             5076..5412
                     /locus_tag="JNY02_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
     gene            complement(5409..5675)
                     /locus_tag="JNY02_002670"
     CDS             complement(5409..5675)
                     /locus_tag="JNY02_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBB9434159.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5949..9473
                     /gene="nifJ"
                     /locus_tag="JNY02_002671"
     CDS             5949..9473
                     /gene="nifJ"
                     /locus_tag="JNY02_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBB9434160.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9841..10970
                     /gene="ompN"
                     /locus_tag="JNY02_002672"
                     /pseudo
     CDS             9841..10970
                     /gene="ompN"
                     /locus_tag="JNY02_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11111..11545
                     /gene="uspF"
                     /locus_tag="JNY02_002673"
     CDS             11111..11545
                     /gene="uspF"
                     /locus_tag="JNY02_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBB9434161.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11721..12656
                     /gene="ttcA"
                     /locus_tag="JNY02_002674"
     CDS             11721..12656
                     /gene="ttcA"
                     /locus_tag="JNY02_002674"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBB9434162.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12788..14155)
                     /gene="dbpA"
                     /locus_tag="JNY02_002675"
     CDS             complement(12788..14155)
                     /gene="dbpA"
                     /locus_tag="JNY02_002675"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBB9434163.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14633..15616)
                     /gene="zntB"
                     /locus_tag="JNY02_002676"
     CDS             complement(14633..15616)
                     /gene="zntB"
                     /locus_tag="JNY02_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBB9434164.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15871..17103
                     /gene="dgcM"
                     /locus_tag="JNY02_002677"
                     /pseudo
     CDS             15871..17103
                     /gene="dgcM"
                     /locus_tag="JNY02_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17124..17687)
                     /gene="smrA"
                     /locus_tag="JNY02_002678"
     CDS             complement(17124..17687)
                     /gene="smrA"
                     /locus_tag="JNY02_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBB9434165.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17941..19395)
                     /locus_tag="JNY02_002679"
     CDS             complement(17941..19395)
                     /locus_tag="JNY02_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBB9434166.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19392..>19779)
                     /locus_tag="JNY02_002680"
     CDS             complement(19392..>19779)
                     /locus_tag="JNY02_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBB9434167.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5198 a         5009 c         4667 g         4905 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt ttgtgcagat
      301 gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca ttggggattt
      361 tctgaccaga gtcatttctc aacggtattt aagcaacgct ttgggatgac gccaggcgag
      421 tatcgccgta aattccgcta actaattggc gttgcatgta ctgcaacgcc aattagttaa
      481 aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga gtttttgaat
      541 attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta tcccgatatt
      601 gcggttttgc tattaaaact ttcgcaccca tttaccgttt atcccttgta catattcccc
      661 cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat ctacagacac
      721 attattttgc tttgcccgtt gttggtaact cgctttacgt tcggcattaa tttcggttac
      781 taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa atgtttcacc
      841 tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc tgcttaccag
      901 cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag aaaagatcgc
      961 tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc tcatgctcaa
     1021 ttttaacgtt catattgata gtgatcggtt ccttaggtgc agcgacttca atgcgaggcg
     1081 tacagccaac cagcataaaa gatgacgtca acgcagccag taaaattttc attgtttttc
     1141 ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct gctccaacca
     1201 tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat tctcttcatg
     1261 atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac ctgtaatgtt
     1321 ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg accggctaat
     1381 ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt tcaccaccgc
     1441 atctgcggta tctttatcca gccgcaaggt cattggcccg ctatttgcca gccaaccatc
     1501 tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa atgccccgga
     1561 catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga gattattcag
     1621 ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagca cgttaccgcc
     1681 taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc acggataacg
     1741 cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt cggcaattcg
     1801 caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac ggaaaggcag
     1861 gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc ctttcaacac
     1921 gccgtgccct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa cctgagcata
     1981 cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct ggaatacagt
     2041 cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc agcgaccatt
     2101 tacccgaacc gggccaatct caccaacatg taaatcgcct ttaaagagga aataggtcgg
     2161 atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc cgccagtgaa
     2221 cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct gtgcgtcacg
     2281 tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca tcgtaccgta
     2341 ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt catgccactc
     2401 accttttcct gcgacatccc aggtggcatt catcggtgta aaactccctt ttccccagta
     2461 gcgccactgc cagcggccag cgtcagggag aaaatcattc gccagcccat ccatatgcag
     2521 cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac gtccgtcaac
     2581 accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga tgtccagcga
     2641 atctatgact cttccctttg aacgaagtaa cgcgcctggc tcaaaggtca gcgttgggtc
     2701 agacagactt ccacttagct gcgcaggtaa acgggcatat aaaatgaggt ccgcctgttt
     2761 cgcttcaccg gtcagctgca gaggcagttg actgttatcc atgcttaatt ttcctgggcc
     2821 aaaattaagc accgcgttgc ccttacccgc ttgcccctgg gtcagcacac tcagtcgtcc
     2881 gctgaccaga gcgttctcaa gccctgcctg ccagttgtca actttgacat ccagtcggcc
     2941 actcaaagga aaacctgcat acagccagct ccagcgacca tcgcttacgg tcaattgttg
     3001 acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg ccagcacgat
     3061 caattgcccg ctattttcct gccagtccag ctcggcatcc accagtgacg gttcctgcgg
     3121 caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca cgagcggcat
     3181 agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga catccagttc
     3241 gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt cgccctgata
     3301 acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc attcctgcca
     3361 gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg actgccactg
     3421 cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg ccagacaagc
     3481 agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg aaaggctggc
     3541 gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg gaatgattaa
     3601 acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga gcggtagcca
     3661 gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc agcagcgtca acggcacaag
     3721 aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg gtagttaata
     3781 tcctgatttc gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt taaccgttca
     3841 gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata tcgccatggc
     3901 tttcaattat atttgaaatt ttgtaaaata tttttagcag cttaaatgtg attcaacatc
     3961 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
     4021 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
     4081 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
     4141 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
     4201 tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag aactggggct gaaagtagtc
     4261 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
     4321 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
     4381 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
     4441 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
     4501 tatccaagtg cagcggtgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
     4561 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
     4621 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
     4681 gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
     4741 ggtatggacg tctatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
     4801 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
     4861 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
     4921 agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     4981 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     5041 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     5101 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaaa
     5161 tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt agcggtgaag
     5221 gcaggtgaac tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag
     5281 cttaatgaac tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg
     5341 accgcgaacc agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat
     5401 ttaatgaatt aatagctgcc acagctcccg gcggcaagtg actgttcgct acagcgtttg
     5461 ccgttgggta atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc
     5521 gaaccgccga tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaactt
     5581 ggcgctacgt gcgccgcagt cgcctgctga acaggctcac tactacacgc cgacaacaat
     5641 aaagcggcac accctaccca aaacgctgct cgcatctctt cttcctctga tcttcaagcc
     5701 aaacgacacc gccataaata ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc
     5761 gttgccccgc atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat
     5821 tcattactct gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa
     5881 ttgaaggatt actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag
     5941 ggtgtcatat gattactatt gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca
     6001 gtgaagttat cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg
     6061 cctgggccgg aaacggcttg aagaacgttt ggggagacac accacgcgtg gttgaaatgc
     6121 agtcggaagc gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa
     6181 catcgtttac gtcatcgcag ggtttgctgc tgatgatccc gacgctgtac aaactggcag
     6241 gcgaactgac gccatttgtc ctgcatgtag cggcacgtac cgttgccaca catgcacttt
     6301 ctatttttgg cgatcattcc gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt
     6361 gtgcggctaa cgtccaggaa gcgcaagact ttgctctcat ttcgcacatc gcaacgctga
     6421 agagccgcgt gccatttatt catttctttg atggttttcg cacgtcccac gaaatcaata
     6481 aaattgtccc gctggccgat gacactattc ttgatctcat gccgcaggcc gaaattgatg
     6541 ctcatcgcgc ccgggcgctc aacccggaac atccggtgat ccgcggtacg tccgccaatc
     6601 ctgacactta tttccagtct cgcgaagcca ccaacccctg gtacaacgcg gtctatgacc
     6661 atgttgaaca ggcgatgaat gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg
     6721 agtattacgg gcatccgcaa gcggaacggg tgattatcct gatgggctct gccattggca
     6781 cctgtgaaga agtggttgat gaattgctaa cccgtggcga aaaagttggc gtgctgaaag
     6841 ttcgtctgta ccgtcccttc tccgccaaac acttgctgca agctctgccg ggatccgtac
     6901 gcagcgtggc ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc
     6961 tggatgtgat gaccgcactg gcaggagcct ttaataatgg tgagcgcgaa accctgcccc
     7021 gtgtcattgg tgggcgctat ggtctttcat ccaaagaatt tggcccggac tgtgtactgg
     7081 cggtatttgc cgagctcaac gcggctaaac ctaaagcacg ctttacggtt ggtatttacg
     7141 atgatgtgac caatctgtca ctgccgttgc cggaaaacac tctgccaaac tcggcgaaac
     7201 ttgaagcctt gttttatggc cttggtagtg atggcagcgt ttccgcaacc aaaaacaata
     7261 tcaagattat cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca
     7321 aaaaagcggg cggcctgacg gtttctcacc ttcgcgtgag cgaacagccg attcgttccg
     7381 cttatctcat ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat
     7441 atcagatggc tgagcgttta aaacctagcg gcattttcct gcttaatacg ccgtacagcg
     7501 cagatgaagt gtggtcgcgc ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag
     7561 cgcgcttcta tgtgattaac gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta
     7621 ttaataccgt catgcagatg gcttttttcc atctgacgca aattctgcct ggcgatagcg
     7681 ccctcgcaga attgcagggt gcgattgcca aaagttacag tagcaaaggt caggatctgg
     7741 tggagcgcaa ctggcaggct ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc
     7801 aaccggtaaa tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgatt
     7861 tcgtgaaaac cgtaaccgcg gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg
     7921 cgctgccgcc agacggcacc tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg
     7981 ccgaagagat ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg
     8041 cttgcccaca ctcagctatt cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc
     8101 ctgccagcct gcattcgcta gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct
     8161 tgcaggtggc accggaagat tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga
     8221 aagaccgtca gaatccagag attaaagcca tcaatatgat gtctcgccag gaacatgtcg
     8281 aagaagagaa aatcaattac gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac
     8341 tggaacgtat tgatattcgt acatcgcagc tgattacacc gctgtttgaa tattcaggtg
     8401 cttgccccgg ttgtggcgag acgccgtata ttaaattgct gactcagctc tatggtgacc
     8461 ggatgttgat cgctaacgcc accggctgtt cttcaattta tggcggtaac ctgccctcta
     8521 caccgtatac caccgatgcc aacggtcgtg ggccggcatg ggcgaactct ctatttgaag
     8581 ataatgccga atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc
     8641 tgcgtctgct ggatcaattt gccgataaaa tcccggcgga attactgacg gcgttgaaat
     8701 cagacgctac gccagaggtt cgtcgagaac aggtcgcagc tttacgccag caactcaacg
     8761 atgttgccga agcacatgaa ctgctgcgtg acgcagatgc actggtggaa aaatcaatct
     8821 ggctgattgg tggcgacggc tgggcttacg atatcggctt tggcggtctg gatcatgtat
     8881 tgagtttgac ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaacaccg
     8941 gtggtcaggc gtcgaaagcg acaccgctgg gtgcagtaac taagttcggc gagcacggca
     9001 aacgtaaagc gcgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg
     9061 cgcagatttc tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag
     9121 cgtatccggg accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc
     9181 tggctctcag ccacgaccag atgcgccaac tcactgctac cggcttctgg ccgctatatc
     9241 gctttgatcc gcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc
     9301 cgtcagaagc actggaagaa acgttacttc acgagcagcg tttccgtcgt ctgaattcgc
     9361 agcagccaga agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct
     9421 atgacttcct agcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct
     9481 ggataaggat tatccaattc taaaaaaaaa agcccggacg actgttcggg cttgtctttt
     9541 tatatgttgg aaaatcagtg gcaatgcaat ggcccaacag aaaataaaat ctgtgacaat
     9601 aaaggcatat aacccgcgca gaataacgta tagaaaatta aaagtataat ttttattttg
     9661 tatgtattat cttcgataca ttcaccagac ttattatcat ttcagaggaa ttatttgatt
     9721 aaggttttac ttaaggcgta acaaatgata tttatcggca aactgaactt ctcctttagg
     9781 atgttttcac tccccgaaat ggggataaaa ggcaaataaa ataactaaag gatttattca
     9841 atgaaaagaa aagcactggc acttttaatc cctgccctgc tcgccgcagg tgctgcacat
     9901 gcagccgaag tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc
     9961 ctgcattatt tttctgataa ttcagcgaaa gatggcgacc agagctatgc gcgtctgggt
    10021 tttaaaggcg aaacccaaat taacgatcaa cttactggct acggtcaatg ggaatacaat
    10081 attcaggcaa acaacactga atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc
    10141 ggactgaaat ttgcagatta cggttctttc gattacggac gtaattatgg cgtaatgtac
    10201 gacatcgaag gctggaccga tatgctgcct aaatttggcg gtgactctta taccaatgca
    10261 gacaacttta tgactggtcg agccaatggc gtcgcgactt atcgtaatac tgatttcttc
    10321 ggtctggtaa atggtctgaa cttcgcggtg cagtatcaag gtaacaacga aggagccagt
    10381 aatggtcagg aaggcaccaa taacgaacgc gatgttcgtc atgaaaacgg tgacggctgg
    10441 ggtctttcca caacatatga tttaggcatg ggctttagcg ctggtgcggc atacacctct
    10501 tctgaccgca ccaatgacca ggttaaccat actgcggcgg gtggtgataa agcagacgcg
    10561 tggactgctg ggctaaaata cgatgctaac aatatttacc tagcaaccat gtattcagaa
    10621 acgcgtaata tgaccccgtt tggcgacagc gattatgcag ttgcgaacaa aacccagaac
    10681 tttgaagtta ctagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
    10741 ctaaaggccg tgaccttcac gctgctggtg gtgcagacaa cccggcaggt gttgatgata
    10801 aagatctggt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
    10861 cctatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg
    10921 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
    10981 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
    11041 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
    11101 aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttcagatt cagaattaac
    11161 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gatgatgcag aggttcattt
    11221 cctgacggta ataccttcac tgccctacta tgcctctctg ggtttagcgt attccgcaga
    11281 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
    11341 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
    11401 ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
    11461 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
    11521 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
    11581 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt
    11641 tatatcagat ttcttatgct gagcgttccg gcatgacgca tactcttctg atgccatata
    11701 acgaattgag tcgcttttaa atgtcgcaaa atcaagaaat tagtaagaaa gaacaataca
    11761 acctgaacaa attacaaaaa cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca
    11821 atatgattga agaaggcgat cgcatcatgg tttgcctctc cgggggtaaa gacagctata
    11881 ccatgctgga gattctgcgc aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg
    11941 ctgttaacct cgatcaaaag caaccgggct tcccggaaca cgttctgccc gagtatcttg
    12001 aaaagctggg cgttgagtac aagattgttg aagagaacac ttacggtatc gtgaaagaga
    12061 agattccaga gggcaaaacc acttgctcac tgtgttctcg gctacgtcgc ggtatccttt
    12121 atcgcaccgc aacggaactg gggacgacga agatcgcgtt gggtcaccat cgtgacgata
    12181 tcctgcaaac gttgttctta aatatgttct acggcggcaa gatgaaaggt atgcctccga
    12241 aactgatgag cgatgatggc aaacatatcg ttattcgtcc actggcctac tgccgcgaga
    12301 aagatattca gcgatttgcc gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg
    12361 gttcacagcc taacctgcaa cgtcaggtga ttgctgacat gttgcgtgac tgggataaac
    12421 gttatccagg gcgtatcgag acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc
    12481 tgtgcgatac caacctgttc gatttcaaag gcattaccca cggttctgaa gtggttaacg
    12541 ggggtgatct ggcgtttgat cgcgaagaga tcccactaca accggcgggc tggcagccag
    12601 aagaagatga aaatcagttg gatgagttac ggctgaatgt ggttgaagtg aaataaccag
    12661 gatagcgccc gatgcgcaag cgtatcgggc tactcttatg gaggccggat aagacgcggc
    12721 cagcagcgtc gcatccggca atcccgaata agatgtttac tcttgcaccc ggcaattcaa
    12781 catttcatta ttttaataac cgcacccggc acgtttttcc tttaatcttc ccgccctgta
    12841 actgtttcca tgctttatga gcaacagcct gacggaccgc gacatagaca tgcgccggat
    12901 gcacggcgat tttgccaata tctgcgccat caagcccgat atctcctgtc agtgcaccta
    12961 atacatcacc cgggcgcatt ttggcttttt tcccgccatc gatacacaac gttgccattt
    13021 ctgcttccag cgtcacaatg gaactattag ctggcggcgt ttgccagtta agttttatct
    13081 gcaacatgtc agaaatgata ttggcccgct gtgcttcttc cggagcacag aaactgatcg
    13141 ccagaccgct atttcctgca cgagctgtac gaccgatgcg atgtacatga acttcagggt
    13201 cccacgccag ctcaaagttc accaccagct caagcgattt aatatccaga ccacgcgcag
    13261 caacatcagt cgcaaccagt acacgggcgc taccgttagc aaaacgtacc agggtctgat
    13321 cgcgttgctc cagatcgccg tgtaacgaca atgcactttg ctctacttca ttcagcgcgt
    13381 cgcagacagc ctggcaatct tttttggtat tgcaaaacac cacacaagag gatggctgat
    13441 gcaagcttaa taaccgttgc aacagaggaa ttttgccttt actggatgtc tcataaaatt
    13501 gttgttcaat gggtggcaaa gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt
    13561 gcactcgtcc gctgattgcg gcgatggctt ccggccaggt tgccgaaaac agaagcgtct
    13621 gtcgagatgc aggcgcaaaa cggatgacat catcaatggc atcgctaaat cccatatcca
    13681 gcatgcggtc ggcctcatcc atcaccaaag tattcagcgc atccagtgat accgtgcctt
    13741 tttgcaagtg atccagcaaa cgacccggcg ttgccacgat aatatgcggc gcatgttgca
    13801 acgaatcacg ctgcataccg aacggttgac caccgcacaa cgtcaaaatt ttggtatttg
    13861 gcagaaaacg cgccagccga cgcaattcac ctgccacctg atccgccagt tcacgcgtag
    13921 gacacagcac taaagcctgg gtttgaaata gcgacgcatc aatttgctgt aacaagccga
    13981 ggccaaaagc cgccgttttg ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg
    14041 caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat ggttaaataa cccaactcat
    14101 taaggttcgt gagttgggcg ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct
    14161 attctcgtgg tcatcgacgc aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg
    14221 atgcgacaat ttaatcggtt cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg
    14281 gatcggatcg ggcactggaa caggatcgcc aggaatcggt tcagggacag gaatttgcaa
    14341 ataaataagt gtcgtcatat ttccctccgg tcattgggtg gactcttaaa gggtagacgc
    14401 tgataaataa caggcaaaaa aaagccgact catcaaagtc ggcgtcgtac gaatcaattg
    14461 tgctatgcag taattcaaaa aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg
    14521 acgttgcatt cacctgcaag ggagatattg ccctgaatgg gtagagagtt tattgacttc
    14581 gctcaaactt tgcggcgttt ttgtatacag acagccggaa aaattgcttt tgttacaacc
    14641 atttactacg gtgcaaccat aaagcaacac caccaataag aacaactaac agaatacaaa
    14701 aaattgaaaa tccgaattgc cacccgccgc cagggatccc accaaggttg acgccaaata
    14761 acccggtcag aaaggtactg ggtaaaaaga ccattgccat caacgacatt gtataggtac
    14821 gacgagctaa attttcctgc atcacctgag cgatttcatc cgccatcacg ccagtccgtg
    14881 ctatacaggc gtcgatttcg tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc
    14941 gacggcgttg gtcatcgctc atccacggca aacgttcact ggcaagacga gcataaacat
    15001 cacgttgcgg tgccatatag cgacgcatca caattaattg tttgcgcagc agagccagga
    15061 atccacgcgg tggaatttgc tgatcaagga gattatcttc aaggtcgata attttatcgt
    15121 gcagctgctc gataaattca ctggaatgat cggtcaacgc atcgcacaca tccaccagcc
    15181 atcccccgca atcggtcgga cccgtgccct cttccagatc gctcaccaca tcgtccagcg
    15241 ccagcacttt gcgttgtcgg gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg
    15301 cgaccagttg atcggggcgt tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg
    15361 tgccttcacc gaaacggctg actcggggcc gtgtgctctc gcccgccagc gcatcacgta
    15421 cgttattggg aagcagcggt gttgtcgcca gccattgggc gctatcatgg tgtacataat
    15481 taaggtggag ccagcaggga tgcgcttcat caatcacatc tgtattttcc agcggtttaa
    15541 cgccgcctct accatccagc atccaggcaa atactgcatc cgggacatta acgtccgatc
    15601 ccttaatcgc ttccacagtg cctccatcat caacgcatta ttttgtagtc tagccttctg
    15661 gccctgttac gcaacatctc atcaccccat taccctgaaa tgattaataa aattctgtct
    15721 aaattgaata caaaaagcaa aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag
    15781 cgtctatcat tatcagaatt atctgatcat atgacgtggc ttttttgcga tcggatagca
    15841 acaaaaattg ataaaaataa cgggatctca atgattacgc acaacttcaa taccctggac
    15901 ttactcacca gtcctgtctg gattgtttcg ccctttgagg aacagttaat ttatgccaat
    15961 agcgcggcgc gactgttgat gcaagacctc acgtttagtc agctacgaac cggaccctat
    16021 tccgtctcct cacaaaaaga actgccgaaa tacctctccg atctgcaaaa ccaacacgat
    16081 attatcgaaa tcctcactgt tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt
    16141 gttttgcgag agctgacaga aacagaaccg gtgattattt tcgaaggtat cgaagcgccg
    16201 gcaacgctgg gtttaaaagc cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt
    16261 tatgcgcgct tttttctgac taactctgca ccaatgttgt tgattgaccc gtcacgagat
    16321 ggacaaatcg tcgatgctaa cctcgccgcg ctcaatttct atggttataa ccatgaaacg
    16381 atgtgccaga aacataccta ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg
    16441 catgaaattt cgcatttacc cggcggtcat aaccctttga attttgttca taaactggcg
    16501 gatggttcta ctcgtcatgt gcagacctat gccggaccga ttgaaattta tggcgacaag
    16561 ctcatgttat gtattgtgca tgatattact gagcaaaaac ggctggagga gcagctggaa
    16621 catgctgctc accatgacgc gatgaccgga ttactgaatc ggcgacagtt ttatcacatt
    16681 acggaaccag gccaaatgca gcatctcgcc atcgctcagg attacagctt gttgctcatc
    16741 gacaccgatc gttttaaaca cattaacgat ctctatgggc attctaaagg tgatgaggtg
    16801 ttatgcgccc tcgcccgccc cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt
    16861 tggggaggcg aagagtttgt cttattgcta ccaagaaccc cactggatac cgcgctttcg
    16921 ctggctgaaa ctatccgcgt aagcgtggca aaagtgagta tttcgggctt accacgcttt
    16981 accgtcagca ttggtgtggc gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa
    17041 cgcgttgatg atgctttgta tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca
    17101 taagccgcgg atgcgtctcg agatcaacga ctgcgcttag cgtggcgctc ccagttttct
    17161 tgcttcgcct gcgccgtttt acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc
    17221 ggtagcgcgg tgcaatatgc ctgaacatca tcaaattcgg tcagccagcg cgccacatag
    17281 ctgcggacaa tattggcatg tgatttatct tcccgccctt taccatgaat aatcagcaca
    17341 ttacgcaaac catccgccag ggcttgttga atgaaactga acaccatttt gcggcactct
    17401 tccaccggct ggcgcaaaag attcaggctc gcctgttgcg gatatttacc actgcgcagc
    17461 ttatccagca ccccatgctg caacccttcc tgccgaaact ccagcggctg acttagcggg
    17521 atgatgtcga gaaatccggt agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc
    17581 ggcgcacgtt ggttacgcgt tggatgccag tggacatctg tagcacgttt cagcggctgg
    17641 acatcttcca tggcgtcaag aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg
    17701 caccttcaca cgtcgtgatt cagacgggtt aattgttcat acttactgca tcgttccctt
    17761 cacagattga aacaatactc tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt
    17821 atactagcct gttagcagta tcgaacagtt tagcccgagt ttaattcagc acaggctccg
    17881 atcaggatga tttattcata gcaggaacca acaaaaatgc gataaataac agaccactga
    17941 ttagcagaaa ggtaccgaaa atgccatagt ttgcgcctcc ctcagcatca attgactcaa
    18001 taatttggtt gaagccagta tttccgatgt ctggtcactt ctactgcgat taaaaaataa
    18061 agcacttctg gaaatgaagt cattcaccgt atggggaaca gaaaccattt tttcacccat
    18121 tctcgaaaaa tgaaaattag ttcgattatt gagaatagag ccacacaggg caataccaat
    18181 cgcgccccca agattacgtg ttaaattaaa tacaccagag gctaacttca gccgttcttt
    18241 tggaattccc cccagcgtta atgttacgat gggggccatc gcaaactgct gcaaaatccc
    18301 tctaattgcc tgtggaaaca gtaattcctg ccatccccat tcgtgggtta taggggtaaa
    18361 aagatacatt gaaaacacaa atccgcccag ccccgccatc aacagccatc gcagatttat
    18421 ttttttactt aaccagaagt aaaagggaac agaaaataac tggaaaatac ctgttgtgca
    18481 aacggcaaaa cctatctctt cggcatttaa tccgcgaatc tgccccaaaa acacaggtat
    18541 taaatataca gtagagaata tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt
    18601 gaaattttta tccttaaaag catgaagatc cattatcgga ttggatattg taagtgtacg
    18661 cgcagcaaat aaaataaaac tcaccaatgc cagaactgat gtcagcaaaa tagtattatc
    18721 atccagccat ccccagcgcg ctccttcttc aagggtatat tccagacatc ccagagtcat
    18781 ggccaacagg ataatactcg gataatcagc aactttcagt aaagagagat caggtttatc
    18841 aaaattaacc agaaaaggaa tactcaaaac taaatatatg ccaggtaata tattgatata
    18901 aaatagccat cgccagtcga ggttatcagt gagccagccc cccagagttg gccctagcgt
    18961 cggtgacagt gaggccagcg cactgactac cgcagcagcc aaccccaact ctttaccctg
    19021 gtaataaata aacgccgtcg tgaaaactaa agggatcatg gatgcgccag cagctccctg
    19081 taaagcacga aacaaaatca tgatctgaat attccatgca agtccacacg cgatactcat
    19141 taaagtaaat attcccgcgg atagagtaaa aagccaacga gtggaaaata cccgtgacag
    19201 ccacccagag agcggaataa caataatctc agcaattaaa tagctggtct gaagccaggc
    19261 catttcgtct ttacctgcgg acagaccacc acctatttca tttagggatg acgatacaat
    19321 ttgaatatca agctgagcaa taaataatcc catacacata ctggcgaaag caatatgttt
    19381 acgcatagac atcatctctc atcaacactg acggtgacgg acagtcccgg gcgaagaagt
    19441 tgctttaatt cctttgcgtc gtcaaatgca atccgtaccg ggacacgctg aacaattttg
    19501 gtgaaattgc cagtggcatt ttctatcgga atcaagctga aactggctcc tgtcgctggg
    19561 gaaaggcttt caatgtgacc gtgaaacact tcccctttga gaatatcagc acggatctca
    19621 gccttcattc ctggcttcat tcccgctatt tggttttctt tataatttgc atcaacccac
    19681 aactcactga ctggtactag tgaaactaac gatgtgccgc cttccaccca actgcctgta
    19741 tgggcgctgc ggtttgcgac aatgccatca atcggcgca
//
LOCUS       DADWTH010000071        19418 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.071, whole genome shotgun sequence.
ACCESSION   DADWTH010000071 DADWTH010000000
VERSION     DADWTH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.071"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..352
                     /locus_tag="JNY02_002681"
     CDS             <1..352
                     /locus_tag="JNY02_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434168.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            509..1258
                     /locus_tag="JNY02_002682"
     CDS             509..1258
                     /locus_tag="JNY02_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBB9434169.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1280..1846
                     /gene="yieF"
                     /locus_tag="JNY02_002683"
                     /gene_synonym="chrR"
     CDS             1280..1846
                     /gene="yieF"
                     /locus_tag="JNY02_002683"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBB9434170.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1900..3237)
                     /gene="adeP"
                     /locus_tag="JNY02_002684"
     CDS             complement(1900..3237)
                     /gene="adeP"
                     /locus_tag="JNY02_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBB9434171.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLANEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3401..4066
                     /gene="yieH"
                     /locus_tag="JNY02_002685"
     CDS             3401..4066
                     /gene="yieH"
                     /locus_tag="JNY02_002685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBB9434172.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4133..4606
                     /locus_tag="JNY02_002686"
     CDS             4133..4606
                     /locus_tag="JNY02_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBB9434173.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4655..5242
                     /locus_tag="JNY02_002687"
     CDS             4655..5242
                     /locus_tag="JNY02_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBB9434174.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGMKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5304..6026)
                     /locus_tag="JNY02_002688"
     CDS             complement(5304..6026)
                     /locus_tag="JNY02_002688"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBB9434175.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6028..7203)
                     /locus_tag="JNY02_002689"
     CDS             complement(6028..7203)
                     /locus_tag="JNY02_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBB9434176.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7230..8846)
                     /gene="bglH"
                     /locus_tag="JNY02_002690"
     CDS             complement(7230..8846)
                     /gene="bglH"
                     /locus_tag="JNY02_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBB9434177.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8932..10329)
                     /gene="bglB"
                     /locus_tag="JNY02_002691"
     CDS             complement(8932..10329)
                     /gene="bglB"
                     /locus_tag="JNY02_002691"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBB9434178.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10345..12222)
                     /gene="bglF"
                     /locus_tag="JNY02_002692"
                     /pseudo
     CDS             complement(10345..12222)
                     /gene="bglF"
                     /locus_tag="JNY02_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
     gene            complement(12356..13192)
                     /gene="bglG"
                     /locus_tag="JNY02_002693"
     CDS             complement(12356..13192)
                     /gene="bglG"
                     /locus_tag="JNY02_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBB9434179.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMCLAINIERVRK
                     EH"
     gene            complement(13478..14203)
                     /gene="phoU"
                     /locus_tag="JNY02_002694"
     CDS             complement(13478..14203)
                     /gene="phoU"
                     /locus_tag="JNY02_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBB9434180.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
     gene            complement(14218..14991)
                     /gene="pstB"
                     /locus_tag="JNY02_002695"
     CDS             complement(14218..14991)
                     /gene="pstB"
                     /locus_tag="JNY02_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBB9434181.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(15083..15973)
                     /gene="pstA"
                     /locus_tag="JNY02_002696"
     CDS             complement(15083..15973)
                     /gene="pstA"
                     /locus_tag="JNY02_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBB9434182.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(15973..16932)
                     /gene="pstC"
                     /locus_tag="JNY02_002697"
     CDS             complement(15973..16932)
                     /gene="pstC"
                     /locus_tag="JNY02_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBB9434183.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(17019..18059)
                     /gene="pstS"
                     /locus_tag="JNY02_002698"
     CDS             complement(17019..18059)
                     /gene="pstS"
                     /locus_tag="JNY02_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBB9434184.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(18307..19380)
                     /locus_tag="JNY02_002699"
     CDS             complement(18307..19380)
                     /locus_tag="JNY02_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9434185.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
BASE COUNT         5061 a         4927 c         4505 g         4925 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcagg gcgctggaca atgtgttaca
       61 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
      121 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
      181 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
      241 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
      301 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
      361 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
      421 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
      481 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
      541 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
      601 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
      661 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
      721 tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
      781 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
      841 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
      901 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt
      961 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
     1021 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     1081 cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt
     1141 agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
     1201 cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata
     1261 gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     1321 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     1381 catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     1441 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
     1501 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     1561 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
     1621 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     1681 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
     1741 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
     1801 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     1861 agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac
     1921 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     1981 cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
     2041 cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
     2101 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc
     2161 agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa
     2221 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
     2281 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
     2341 actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat tcgccagacc
     2401 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa
     2461 agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
     2521 aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat
     2581 ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga
     2641 ggccagaata gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag
     2701 attaccgatg ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttcagccc
     2761 catcatgccg ataaacaggc cgataccgct ggtaataccc acacgcagac ttaccggaat
     2821 gttagcaatc atccagtaac gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc
     2881 ccagaagatt gcgcccatac cgatctgcca cggcagcccc attgcctgga caaccacaaa
     2941 agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa
     3001 tcccatcata atgctaccga aagcagcaat cagacaggtt gttacgaaga cggcactggt
     3061 gtccatgcca gcaacgccca gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag
     3121 gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt
     3181 aaaaacgcgt tccagcatcc tctgaccaga agtctgggtg gtatgttgat gactcattat
     3241 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatgc gacaataggc
     3301 gcgtttgtga gagacttttt attggattta cttatacggc aacgattgcg ttgcgcaaat
     3361 cggctctacg aaaacgttaa actgattaaa aaggaaaggc atgtcccgga tagaagcggt
     3421 atttttcgac tgcgacggta cgctggtcga cagtgaagtc atttgctccc gcgcatatgt
     3481 aacgatgttt caggaatttg gtattacgct cgatcctgaa gaggtattca aacgtttcaa
     3541 aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt gaacatggtg ttacgttagc
     3601 gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct cggctgttcg attcagaact
     3661 ggaagccatc gaaggggctg gagcgctcct gtcagcgatc actgcgccaa tgtgtgtggt
     3721 atctaacggc ccaaataaca aaatgcagca ttctatgggc aagctgaata tgttgcacta
     3781 cttcccggat aaactgttca gcggctacga tattcagcgc tggaagccag acccggcgtt
     3841 aatgttccat gcggcaaaag cgatgaatgt aaatgtagaa aactgcattc tggttgatga
     3901 ctcagttgcc ggtgcgcaat ctggtatcga cgcaggtatg gaagtgttct acttctgcgc
     3961 cgacccgcac aataagccga tcgttcaccc gaaagtcacc acctttaccc atctttcgca
     4021 gttaccggaa ctgtggaaag cgcgtggttg ggatattacg gcatagttct tcacactccc
     4081 ttcacttacc ccgcttaaat tggcgctcaa aggtaagtaa agggagtttg atatgtctgt
     4141 ttcacgtcgg gtaatacatc acggacttta ttttgcggtt ttaggaccgt taattggtgt
     4201 tctgtttctt gtcctctaca tattcttcgc aaaagaaccg ctggttcttt tggtaataat
     4261 acaggtactt ccattgtttc tcttattgtc gattactacg ggagctattc ctgcattgtt
     4321 aaccggcgtg atggtcgcct gtctgccgga aaagatcggg tcacagaaaa attatcgttg
     4381 tctggctggt ggcataggtg gcgtcgttat caccgagatc tattgtgcag ttattgtaca
     4441 tattaagggc atggcttcct cggagttgtt tgaaaacatt ctttctggtg acagtctcgt
     4501 tgtccgcatc attcctgcat tgctggcagg tgtggtgatg agcagaatca ttacccgtct
     4561 gcccggattg gatatttcat gtcctgaaac agactcttta agttaagcgg gatactttat
     4621 ctttgggcta ctcaaaagca gacaggatgt tactatgact caaaatatca ggccgttacc
     4681 ccaattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg ataaaaccgt
     4741 agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct tttattgcgt
     4801 tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg ctgaaaagtt
     4861 tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt atgatgaaga
     4921 ggacgaattt gccggtatga agaacacata tcctgatgaa atgctgaaag agttggttga
     4981 acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt gtggcgattt
     5041 ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg atgaatttgc
     5101 caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag ctaaatgcct
     5161 gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg gcaagctgag
     5221 actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag cgtaattcat
     5281 acatccggcc ttatttcttc agcttaaccg aacgctaatt ctgcggcagc ggctttatca
     5341 gcgattacca tcagcgaagg atgcagctgc aacacggaag caggaacatc ttcggtaacc
     5401 ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac aattatcagc
     5461 aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc aggcactaac
     5521 gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc ctggatcggg
     5581 aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca aaaatgacca
     5641 tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag tttctgatca
     5701 tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat ccccgcaggg
     5761 gtgaaaaaca gattacgcag attggtaatc gttacgcctt ctccctcttt gccgcgaaag
     5821 ggaatttcat cgaaattata gaaatagcag ttatcgtacc aaggcttacc tttaaccagg
     5881 gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc caggttaaca
     5941 cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct catttcctgg
     6001 taatcttcgg taatgattaa tttcatctta atgccttatt ttaaatttct gtacgaaggc
     6061 tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt cctggtaatc
     6121 aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct tgccggttac
     6181 gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa tagctgccgg
     6241 atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa caccactgaa
     6301 tgtggccagc cagccaaagc tttccagatg attcattccg gaaaccagcg cctggtaccc
     6361 gccttgtgaa agccctgcca gcgcgcgacc atcggcatct ttacggacac taaaacgctt
     6421 gctaatcagc gggataatat cgttcatcag ttcgcgatct gccgctttag cattcagcgg
     6481 ataaaagact ttacgtcttt cctgaggcac gaaatcttcg ggaataatgc ccttcgcatc
     6541 ggtttcggta tccgggatca ccaccagcat cggtttaatt ttcccttcag ccagcaggtt
     6601 atccatgatt tgcgggatac gcccctgatc gatagcggaa cgtccagtat caccaaaacc
     6661 gtgatagaaa tagagcactg gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca
     6721 gacatacatc tgacgttcag attgcaatgc gttggagtgg taagttatgg cgatcaaatc
     6781 accatgcgca acagaacgcg tatccagata actgcctagc accagaatca tactggagtt
     6841 aacctggcgc tgaggcttag tcattgcggt gcctgtatca atgctgcgta caccatcaac
     6901 attgaaaaaa tactcgtaca ggttgccttt caggataggt gtgcgccacg accagactcc
     6961 tgcttcgtct ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca ccactacaga
     7021 gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg cattgacctg
     7081 agtcacatac tgtttcaccg ggatagtggg tgccgggctg gctggcatat ccgctgctat
     7141 ggcccactga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa tttttatatt
     7201 catcgtaata ttcctttcat taagttccat taccaccaga tttcagcctg ggcaccgaca
     7261 gcaaactggt cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt ttccagggct
     7321 ttgatatacg tggcgtagaa acgaatttcc agacgcgagg tcaacatact ggtattgact
     7381 ttaaaggtat ggaagagcgt ggttttataa ccagactcat taaatttatt actattcgca
     7441 tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata ttggtcccaa
     7501 atataggccg ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg
     7561 tagctataaa tatcgttacc gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg
     7621 ccgatatagg cttcgccctg cgaggtcaga cgaactgctg ttccgccggt gtgatcacca
     7681 taataacgac cattaaaggt ggtaaatgga cttgcgccag cataacggcc aaagttcctg
     7741 gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccttt atcaaatttc
     7801 tgcgttaaag atgtgccgaa catccaggta tctttccagt cataataccc gttattatcc
     7861 tgattatctt tttccgatgc gctttcgttt gccgtgacat aacgaccact caccattaag
     7921 gtcgctttat cccataacgg gatatcttta tagcgtaaat caatagtatg ggtattaatc
     7981 tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata
     8041 tcaattttac ccggaccgac tttccagttt tccagaccta cacccgctgc ggcatcagta
     8101 cgctgcgttt tccagtccag catctggatt tcaattttcg gcgcaccgtg tttacccacc
     8161 cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacgta catatcggag
     8221 aactgcataa agttctcgcc accggcgtta tcgccaaacc agccggtaga gtactgctga
     8281 ccaacgttac catccatcat cacaacggca tcaacccgtt tgccgttttc gttgtagaca
     8341 cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg gcccagagaa
     8401 ccaatcgccc atgatttagg tgaaccatga gaggcggtcc cccaaccaga acggtaatag
     8461 ccgttgtaac taaagccaat ttcatctttc acaaacttac tgaaatcttt cagagtcatg
     8521 gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttgac cagtacagca
     8581 tcaggttttg cggcactact ggtcgggaac ggattggcgg catacccttg atcattcgta
     8641 cttacgctac gattcaccgt cgctggcgta tattttttct tctcttcatc tttatacttt
     8701 ttgagttcgc tttgcgtttc tcttaacgct ttttctaata actcaaggcg ttgttccacc
     8761 gttaatgatt cagccaatga ttgtgcagaa aaggctaacg gtgccattaa taagatggca
     8821 gaggtaataa gatttcgtct aaacataata tccctttatg gtgcaaagaa agaattaacg
     8881 tatcgcatcc agactgttct gaatgcgacg ataattaagg tgctttattg gttatttttt
     8941 taatgacagc ccccgcgttt tgatcacttc tgcataccat ccgaagcttt tcttacgcgt
     9001 tcttgtgagg cttccttcgc cattatcatc acgatccaca taaataaagc cgtagcgctt
     9061 agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg tgtaccccat
     9121 aatatccaca ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc
     9181 aattcgataa tcatcctgta tcgaaccatc cgcttcaacg ctgtctttta cgcctaatcc
     9241 attctcgaca ataaataacg gtttttgata acgatcccaa agcgtattta acagaacccg
     9301 taatccaacc ggatcaattt gccaccccct ctctgaactt ttcagatgcg gattggggat
     9361 catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg aaacacaacc
     9421 agtcatgtaa taactaaaag agatgaaatc tacagtatgt tttaaatctt ctgcgtcact
     9481 ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat agccgggata
     9541 ctggccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt ccatggcctg
     9601 caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc
     9661 gattttcgct tcggggatca ggctatgata ggctttaact gcccgcgcac tggcaaccag
     9721 ttgatgatgg atcgcctgat aaacttccgc ctcgccactc tcttctgcca gccccacgcc
     9781 cgtgaatggc gcgtgtaacg acatattgat ttcattaaac gtcagccata acgctacttt
     9841 atgttggtag cgggtaaaga ccgtgcgggc gtaatgctcg aagtgatcga tgaccgcccg
     9901 attagcccaa ccgccgtagt ttttcaccag cccatatagc atttcgtaat gggataacgt
     9961 taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa
    10021 ccccgcttca ttcggttcgg cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga
    10081 aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg ggtaacggtg
    10141 ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg gttccatttt
    10201 tcccattacg ccatgaggct gtaaatctga ggtcgtgatc cctttgccat cttccagcca
    10261 ggcaccttcc acctgattgg cagctgttgc gccaccccaa agaaatgttt ctggaaatgc
    10321 tttcgtaatt aactcctttt atcgttagcg aatgatggat aacagcggtt cacctgcgct
    10381 tatctgcgcc gtgccgtggg gtaatacgtc tgtaaaatca tcgctattac tgattaatac
    10441 cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag aaatcagccg
    10501 atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag
    10561 ttttacggtg tcgataccga catgaatcag gatctccaca ccatcatctg actcaatgcc
    10621 aatggcgtgt aatgtggcga acaacgaagc aattcgaccc gcaaccggag aacgcacttc
    10681 accaaccgag ggcagaatgg cgataccttt acccagcagg ccactggcaa acgtggtatc
    10741 agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac cgccctgctc
    10801 aggtggtgta ataacctctg gtgttttctc ttgcggggca ccctgcgctg gctaacgttt
    10861 actggtgatg aaatgaagca tcaacgtacc aacaaatgcg caacctatgg caatgacacc
    10921 gccaataacg ctggcccaga cggtgaaatc aatccccgtt gacgggatgg tctgcatgaa
    10981 ggtgaaaata cttggcaaac caaaggagta aactttcgtt tgcgcgtagc caatgatggt
    11041 ggcccccaat gctccactga tacaggcgat aacaaagggg tacttacgcg gcaggttgac
    11101 gccatatacc gctggttcgg tgataccaaa cagacccgtc aacgccgctg atcccgccac
    11161 cacttttttc tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc cgacctgcgc
    11221 cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt
    11281 gatacacagc ggcaccaggc cccagtgcag tccgaacatg acgaagattt gccagaagcc
    11341 gcccattacc gcgcccgcaa atgcaggaac cgcctgataa agccagagat aaccggcggc
    11401 aatcatttcg cttatccagg ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat
    11461 aaccatcaga catagcaatg gtgtgaagaa atttttgatt gccgacggta accacgcatt
    11521 aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa taaccgatga
    11581 cgagtaattc aacaatgtga ccggaatacc caggaaatcc agccccggcg catccgcttt
    11641 ttgcccgttc tcgaaggcag tcagaattaa tggatgcact aacgctccac caatcaccat
    11701 ggcagtaaat ggattaccgc cgaagcgttt gcccgcggtg tatcccagga ttatcgggaa
    11761 gaacccaaac aaggcatcac tggcgctgaa taaaattaaa taagtaccac tttgttcggt
    11821 cgtccactga aaagtgagcg ccagagccag catacctttc aggatcccgg ttgccgccat
    11881 cagaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt ttagcagatt
    11941 acctttctca tcattttccg gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt
    12001 aaccgccagg aagacatcgg ccacatggtt acctatgacc acctgaaact gaccaccact
    12061 ttccaccacc ataataatac cgggggtctt tttcagtacc tcagcttgcg ctttgctttc
    12121 atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc agactcacaa tgttatctgc
    12181 gcccccgact cctgcgacta tttttctggc taactccgtc ataacttgcc ctctaccgct
    12241 ttgcggcaaa actccaaaaa aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt
    12301 ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ctcagtaata atatttcagt
    12361 gttctttgcg cacgcgctct atatttatgg ctaaacacat aatctctgcg ggtgaaattt
    12421 tacgttgata ctgtaaacca ataaaaatgg cgatccgttc cgcacattgc catgcttgcg
    12481 ggtaattttg ttttactgct tgttgtaatg attcatcact atcgttaatt gaagcatgtt
    12541 caagaatacg ccaggataaa aacttcagat gtgtaaccag tcgctgataa ctcaagcttt
    12601 cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttcg cgcattaact
    12661 gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc agatgcatgg
    12721 caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca ataatggtta
    12781 acgcttcttc ccctagctgg aactctttcg ggtaaagccg ctggatatcc cacagcaacg
    12841 ggttgggcag caacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
    12901 gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataagac
    12961 gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt
    13021 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
    13081 cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
    13141 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
    13201 ccttttttat aaacaaaaaa acccgacttc accagtattc tctggttatg tcaggttttg
    13261 cctgcgaatg cagtaacaat ccagtcattt attaatggtt tttataacga acatccaggt
    13321 tcggaaatta atttaattgc gtgcagagaa aatagcaatg cgctattgat aaaaatatga
    13381 ccatgctcgc agttattaac tttgtgtaat tttaggaatt tataaagtta tatataacaa
    13441 atcccaataa ttaagttatt gggatttgtc tggtgaatta tttgtcgcta tcttttcccg
    13501 ccagcagttt atccagctca tcactgccta catgacggaa atcctgaccc ttcacgtagt
    13561 agaagataaa ctcacaaata ttctggcagc ggtcgccgat acgttcgatt gaacgcgcac
    13621 agaacagcgc ggtaagtacg ctcggaatag tgcgcgaatc ttccatcatg taggtcatca
    13681 gttgacgaac aataccttcg tattcctgat cgactttttt atcttcacga taaatacgta
    13741 ccgcttcgtc aatgtccatc cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg
    13801 tatgacggcc cagcgactcc agacttacca gcaacggctg atgctgctgg gagaatttct
    13861 ccagcgcagt acggcagatt ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg
    13921 ttttactgat caccataacc agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa
    13981 tgcgcacgca ggcttcatcg atcgccactt ccatcatgtt gacgttcttg tcgccttcga
    14041 tcacgcgctt cgccagatcg ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct
    14101 gctgctccac catgccgccc atggtcatca cctgcgtgcg gatactttcc agttcggcgt
    14161 tgaactggcc ggaaatatgt ttattaagat tgagactgtc cataacgcac tcctgaatca
    14221 accgtaacga ccggtgatgt agtcttctgt ttgtttcttc gctggcttgg tgaacagatc
    14281 gtccgtgttg ctgaactcaa tcaattcgcc caggtacata aatgccgtgt ggtcggaaca
    14341 acgcgcagcc tgctgcatgt tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc
    14401 ggtgatcagc tcttcaatac gcccggtaga gatggggtcg agcgccgaac acggttcgtc
    14461 gagcagcagc acttccgggc gaatggcgat accacgcgca atacacagac gctgttgctg
    14521 accaccagag agagagtaac cgctctggtg caatttatct ttggtttcgt tccacaatgc
    14581 ggctttggtc aatgcccact gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa
    14641 cagacgaacg ccaaaggcga tgttgtcgta gatggacatc gggaacggcg tcggtttctg
    14701 gaacaccatg cccactttcg cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt
    14761 gtcgccatca agcagaattt caccttccgc acgctgctcc gggtacagtt caaacatttt
    14821 gttgaaggta cgcagcagcg tcgatttacc gcagccggac ggcccgataa acgccgtaac
    14881 ctggtttttg gcgatatcca ggttgatgtt tttcagggca tggaatttgc cgtagtagaa
    14941 gttcaaatta cgaacctgaa ttttactcgg ggcagtttca accatactca ttgcaatctt
    15001 ttcctcatcg tacctgatgc ttttcgctaa tcaggcttac cgtagggccg ggtaaggcgt
    15061 tcacgccgca tccggcaaag tgttaaccgt gtttattctt cgcaaaaaca acgcgcgcca
    15121 gaatgttcag cagcaggacg cacagggtaa tgatcaatac cccggcccag gccagttgtt
    15181 gccattccgc aaacgggctc atcgcaaact taaagatcgt caccggcagg ttggcgatcg
    15241 gctgcatcat gtccgtgctc cagaactggt tggagagcgc ggtaaacagc agcggcgcgg
    15301 tttcaccggc aatacgggca atcgccagca ggataccggt cataatcccg gacaccgacg
    15361 ctttcagcgt aatcgcggag atcatcttcc acttcggtgt acccagcgca taagccgctt
    15421 cacgcaggct gtccggcacc agtttcagca tgttctcggt ggtgcggata acgatcggca
    15481 cctgcaacag cgccagcgca attacgcccg cccagccgga gaagtgctcc atctgtgcca
    15541 ccacgatggt gtagacaaac agaccaacca caatcgacgg cgcggagagc agaatgtcgt
    15601 taatgaagcg aatcacttct gccagccagg atttacgacc atattccgcc agataaatcc
    15661 ccgccataat gcccagcggc gtaccgaata ccgtggccca caaaattaac agcccgctac
    15721 ccgccagggc gttcgccaga ccaccacctt ccgtattggg cggcggcgtc atttcagtga
    15781 acagcgccag cgacataccg tcgataccgc gagtgatggt ggacattaaa atccagatca
    15841 gccagaacag gccgaaggcc atcgtcgcca tcgagagcgt cagcgcaata cggtttttga
    15901 ggcggcgacg cgcctgcatt ttgcggcgag attcagccag cgccgcagtg gtttgcattt
    15961 caaccatagc cattagcgtg ccccctcatt cttagccagg cgcataatca taaacttcga
    16021 tgcggcgagg acgatgaagg taatcacaaa caggatcagg cccagttcca tcagtgcggc
    16081 aacgtgcaga ccggattccg cttccgcaaa ttcgttcgcc agcgcagagg tgatactatt
    16141 gcccggcatg tacagcgagg cgctgtcgag ctggtaggtg ttaccgatga taaaggtcac
    16201 cgccatggtt tcaccgagcg cgcggcccag ccccagcatg atgccgccga taacaccatt
    16261 tttggtgaac ggaagaacga tacgccagat aacttcccag gtggtgcagc caataccgta
    16321 ggccgactct ttcatcatca ccggggtttg ttcgaacaca tcacgcatta ccgccgcaat
    16381 gtacggaata atcatgatgg cgaggatcac gcctgccgcg aggataccga taccaaatgc
    16441 ggggccagag aacagcgcgc caacaatcgg gatattcgac atgatattgc cgaccggctc
    16501 ctgaaagtaa acggcgaaca gcggcgcaaa gataaacagg ccccacatgc cgtaaacgat
    16561 acttggaatg gctgccagca gctcaatggc gatacccagc gggcgtttca gccagccagg
    16621 cgcaagctca gtcaggaaca gggcgatacc gaaactcacc gggacggcga tcagcagcgc
    16681 gataaacgaa gtcaccaacg taccgtagat cggcaccagc gccccgtaga tatcgttcgg
    16741 tgcatcccac tctttggtcc ataggaaagc cagaccaaat ttctgaatgc tcggccagga
    16801 ggagatgatc agagagacaa taatgccacc caacatcaat agcacaatca gcgccgccag
    16861 ttttaccagc acgctgaaaa ttatgtcgcc ctttttaccc ggtgggttaa aagcgggctt
    16921 ggttgcagcc ataagttact cttcagttaa acgcgtttac aaagtcattt tgtaatgccg
    16981 gatgcggcgt aaaacaccgt acccggcctg gagttttatt agtacagcgg cttaccgcta
    17041 ctgtctttaa tattggtctt ccacgcagcg cgaacctgtt caactacact atccggcagg
    17101 ctagcgtaat ccaggtcgtt cgcctgttta gccccggttt tgtacgccca gtcgaagaat
    17161 ttcagcacct ctgtgccttg ttctggtttc ttctgatctt tgtggatcag aatgaacgtg
    17221 gtagaggtaa taggccatgc atcttcgcct ttctggttgg tcagatcctg agcgaaggtt
    17281 ttgctccagt ctgcaccttt tgctgcatta gcgaagtttt cttcggtcgg actaaccggt
    17341 ttaccatcag cggagatcag tttggtgtac gccaggttgt tctgcttcgc gtaagcatat
    17401 tcaacataac caattgcacc cggcagacgc tgaacgaacg cggcgatacc gtcgttacct
    17461 ttaccgccca gaccgatcgg ccattttacg gtagagccag taccaacgtt gtttttccac
    17521 tcttcgttca ctttcgccag gtagctggtg aagacgaagg aagtcccgga gccatctgcg
    17581 cggcgtacta cagcaatgtt ttgtgaaggc agtttcagac ccggattcag tttggcgatg
    17641 gcttcatcat cccacttctt gattttgcct aggtagatgt cgccgagggt tttaccatcc
    17701 agcaccagtt cgccagactt cagccctgga atgttaaccg ccagcaccac gccgccaatc
    17761 acggtcggga actggaacag accttcctga gccagttttt cgtcagacag cggcgcgtca
    17821 gaggcaccaa aatcaacggt attagcgata atctgtttta cgccaccgga agaaccgata
    17881 ccctggtagt taactttatt accggtttct ttctggtaag tatcagccca tttggcatac
    17941 accggcgcag ggaaggttgc acctgcacct gtcaggcttg cttctgcaaa cacagagaaa
    18001 gcactcatcg ataaggtcgc ggcgacaaca gttgcgacgg tggtacgcat aactttcata
    18061 atgtctcctg ggaggattca taaagcattg tttgttggct acgagaagca aaataggaca
    18121 aacaggtgac agttatatat aaggaatatg acatttttat gacagaaagg aattaccaaa
    18181 taacttactg attttaaatg tgaagatgaa aatcccacat gccatctttt tgtcatgatg
    18241 catttaaaag tgataaagaa atgggtgaga tgacaacgat gtgatgaaca atcgttgtca
    18301 gacctattag cgcacaatct caacagttat cgtcgctgtt gcagtaaatt gccccggggc
    18361 tgggcgagct ccagtaaggc tcgcaggagc agctgaaaaa gttacactac ctgatttttc
    18421 accatacact gttgatttat ccatgtctgt tggcaactca ccaccgttca ctttgatttc
    18481 cttattattt ttgtcgtaaa tttttatccc cacgttagag tttgaagtta cgaccattga
    18541 attgtccgta ttgggttcgg tagcataaaa agacgtactg atatcatcgg tattcagtat
    18601 ccctaggttt tcacactcaa tagtgactgt tttagtaatt ttatgtgttg ttaccgcact
    18661 tccaacaaca gatgaaaaat ctgcagctgg tatttcttta aaatcaaaac aaactggctt
    18721 taaattattt atttcgcaac tttgcggagc tgtaatatcg ccccgaatat aaacatcagc
    18781 catcggctgt gagtaatcca ccggggtgtc acggctaatt gttccataca atgtcactat
    18841 ctttgtttca ggaataatca attttcctgc aaatgtcttc ttgatataaa aatatactat
    18901 cgctttagcc ccactggata tacttaaagg ttcaatgcgg ttgcaattgt actttgtatt
    18961 accagacggg ttgttagcat gatattcaaa aggtgcattc acatacccaa ccccaagaat
    19021 atctatagca atcccaacat caaggttcgg attaagatca taataattaa tattagagtg
    19081 aaccccagca taagttaatg cctggtttat ttttgacgta taatagatat aggggaattc
    19141 tcctacgtta ccagtatcac aatgacagcg tccaagatat gaggatccat tagttacagt
    19201 ggtgttaaat gtcgttccaa ccttattttc gttcgcggta ataagatatc ccgagaaatc
    19261 aagcgtactg tgaaatacgc cattatcagg ggtacagata cctttaggag ctgcaagtgc
    19321 tacactactt aacgagagca taaacacaga aaagcaccac tgtttgatat acttgttcat
    19381 aaaaatcctt ttaatgacat gtcacaccct gttcaatt
//
LOCUS       DADWTH010000072        19376 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.072, whole genome shotgun sequence.
ACCESSION   DADWTH010000072 DADWTH010000000
VERSION     DADWTH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.072"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(30..1859)
                     /gene="glmS"
                     /locus_tag="JNY02_002700"
     CDS             complement(30..1859)
                     /gene="glmS"
                     /locus_tag="JNY02_002700"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBB9434186.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2021..3391)
                     /gene="glmU"
                     /locus_tag="JNY02_002701"
     CDS             complement(2021..3391)
                     /gene="glmU"
                     /locus_tag="JNY02_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBB9434187.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JNY02_002702"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JNY02_002702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBB9434188.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JNY02_002703"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JNY02_002703"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBB9434189.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JNY02_002704"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JNY02_002704"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBB9434190.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JNY02_002705"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JNY02_002705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBB9434191.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JNY02_002706"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JNY02_002706"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBB9434192.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JNY02_002707"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JNY02_002707"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBB9434193.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JNY02_002708"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JNY02_002708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBB9434194.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JNY02_002709"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JNY02_002709"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBB9434195.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JNY02_002710"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JNY02_002710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBB9434196.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JNY02_002711"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JNY02_002711"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBB9434197.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHQLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JNY02_002712"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JNY02_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBB9434198.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JNY02_002713"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JNY02_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBB9434199.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JNY02_002714"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JNY02_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBB9434200.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="JNY02_002715"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="JNY02_002715"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBB9434201.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JNY02_002716"
                     /pseudo
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JNY02_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JNY02_002717"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JNY02_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBB9434202.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLKASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4628 a         5120 c         4858 g         4770 t
ORIGIN      
        1 cggcagccct tttttatcta tgatagtgat tactcaaccg taaccgattt tgccaggtta
       61 cgcggctggt caacgtcggt gcctttgatc agcgcgacat ggtaagccag cagctgcagc
      121 ggaacggtgt agaagatcgg tgcaatcacc tcttccacat gcggcatctc gatgatgtgc
      181 atgttatcgc tacttacaaa acccgcatcc tgatcggcga agacatacaa ttgaccgcca
      241 cgcgcgcgaa cttcttcaat gttggatttc agtttttcca acagttcgtt gttcggtgca
      301 acgacgataa ccggcatatc cgcgtcaatc agcgccagcg ggccgtgttt cagctcgcct
      361 gcagcgtagg cttcagcgtg aatgtaagag atctctttca gcttcaatgc gccttccagc
      421 gcgattgggt actgatcgcc acggcccagg aacagcgcgt gatgtttgtc agagaaatct
      481 tctgccagag cttcaatgcg tttatcctga gacagcatct gctcaatacg gctcggcagc
      541 gcctgcagac catgcacaat gtcatgttca atggaggcat ccagaccttt caggcgagac
      601 agcttcgcca ccagcatcaa cagcacagtt aactgagtgg tgaatgcttt agtggacgcc
      661 acgccgattt ccgtacccgc gttggtcatc agcgccagat cggattcgcg caccagagaa
      721 gaacccggaa cgttacagat tgccagcgaa ccaaggtaac ccagctcttt cgacagacgc
      781 aggcccgcca gggtatccgc ggtttcgcca gactgtgaca aggtgatcat caggctgtta
      841 cgacgcacgg cagatttacg atagcggaat tcagaggcga tttcgacgtc gcacggaata
      901 cctgccagcg attcaaacca gtagcgggaa accataccgg agttataaga agtaccacag
      961 gcgaggatct gaatatgctc aaccttcgac agcagttcgt cggcgttcgg tcccagctcg
     1021 cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg tgttttttat cgcgttcggc
     1081 tgttcgtaga tctctttctg catgtagtga cggtaaatac ctttatcgcc cgcgtcatat
     1141 tgcagattgg attcgatatc ctgacgtttt acttccgcgc cagttttatc gaagatgttt
     1201 accgaacggc gagtgatttc cgcaatatcg ccctcttcaa ggaagataaa gcgacgggtc
     1261 accggcaaca gcgccagctg gtcagaagcg ataaagtttt cgcccatacc caggccaatc
     1321 accagcggac taccagaacg tgccgccagc agggtatccg ggtgacggga gtccatgatc
     1381 actgtaccgt acgcaccgcg cagctgcggg atagcacgca gaacagcctc acgcagagtc
     1441 ccgccttgtt tcagctccca gttcaccaga tgggcaatca cttcggtgtc ggtttcagaa
     1501 acgaaggtat agccacgcgc ttttaactct tcacgcagcg gttcatggtt ttcgatgatg
     1561 ccattatgca ccaccacaat gtgttcagaa acatgcggat gcgcgttagc ttctgaaggt
     1621 tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc cgccatgcag aggatgttct
     1681 tccgctgcct gagccagcat ctggacttta ccgaggcgac gcaggcgagt catatgacct
     1741 tccgcatcaa caacggccag accggcagag tcatatccgc ggtattccag acgacgtaaa
     1801 ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg cgccaacaat tccacacata
     1861 gtttttgatt ccgatttata tcgttgtcgg tcaacctgta tgcccgtttt ttcgggcgcc
     1921 ccgagccttg tagagagtgg ggttattttt atagttactg cttgtgggag ggagattatg
     1981 ttatctcctc atcccagatg accggattac ccggccagaa ttactttttc tttaccggac
     2041 gacgccagcc ttctttctga gtctgcggca cacggctgat agctaatgca ttttcgccga
     2101 cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc tttgcctact gttaccgggg
     2161 ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc gataatggtc ttaaatttat
     2221 tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat gttaacgtta tcgccaattt
     2281 ccgcatcgcc taggtaagtc agatgcccgg ctttcgtgcc tttacccaga cgcgcttttt
     2341 tcatctcaac gaagttacca acgtgtgcac cttccagcaa ctcagcacca ggacgcagac
     2401 gggcaaacgg gccaatagta caggccgctg ccagattcgc gtcttccaca acggtatacg
     2461 gactgatttc gcaatcatcg ccaatcacgc tgtttttaat cacgcaaccg gtgccgattt
     2521 tcacgcgatg accgagagtc acgttgccct cgatgataac gttagtatca atttcaacat
     2581 cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc cggatcgcgc agcataacgc
     2641 ctgctaacag cagtttttca gcctgttcgg actggtaaac gcgctccaga cgggagagtt
     2701 gcaggcggtt attcacacct tctacttcgc ttaaacgttg cggatgaacg gcgacgattt
     2761 cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat gtagtattcg ccctgagcat
     2821 tattgttggt cagcttcgcc agccagcgtt tcatatctgc gccgttggca atcagaatgc
     2881 cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc atctttgtgc tcaacaatgc
     2941 cggtaacttt gccattttca cgggtgatac gtccataacc ggtcggatca tccagtttca
     3001 ccgtcagcag accaatgcca ccctggggtt tagcatcacg caggcgctgg agtgtttcga
     3061 cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc ttcatcatcg gcaaagaaag
     3121 gtgcggcctg ctgcattgca tgacccgtac ccagctgctc tgcctgaagc acccagttaa
     3181 ggttgtcgtc tttcaacgcc tgtttaagca gatcgccgcc gtgaccgtac accaggtgaa
     3241 cgtgcgctgc gcctaattca ttcgcagcat caatgacatg ctgaaccatc gctttcccgg
     3301 caagggtatg cagcactttc ggaagatcgg aatacatgcg cgtgcctttg cctgcggcaa
     3361 ggatcactac gctcatagca ttattcaaca tacgcgtcct gactgtaatt tgagaacgaa
     3421 tttaaaccgc ttcaccttga aaaaactaca tttttttcat cgtgaaatgg acagaggata
     3481 aattgttcaa taacggatta tccccgctga cgaaagtgcc attttcgacc atcgtcacgc
     3541 cttttgtctg cagaaaatta aggcagtacc ttcatttaag cgtcaggtgg atgtttttgc
     3601 tcttatttcg atcaatgaat aaacgggaaa acaggggtgt tttatatcag caggatctat
     3661 gtgaacgctc ttcaggacgg gtcaacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacttg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagggcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggagtttat tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgttg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcaag tcgataggca gtaaatccat cagattcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caagaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaagccac ccggcctttc gctggtgtat
    10441 gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct acccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gctggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatca
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtct
    12961 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgca gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacgctaaa gtcgatggtt
    13201 cgagcatcaa tacgcggcgg agtaccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagagagcgg aatggatggc ggatcgccag cacggccacc gctgtaatta
    13321 tccagaccga tatgaatttt gccgtcgagg aacgtcccaa cggtgagcac cacagctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagcgcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cagccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactgccc tgtggataac aaggatccga cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aacggttgtt ctttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctgccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata accagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatggta cgcatgattg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat aacttttata aagatgctgt
    14881 agtggccggt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggtttt ttattgaatg attattgcat gtgtgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttaac gaagagtttg gcctggcacc gttcctgccg gatcagattc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat tgcgaaagat cttggcgcgg tattcctcgt cgggattggc ggcaagttga
    15961 gtgatggtca tcgtcacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggcca tgcgggcctg aacggcgata ttctggtgtg gaacccggta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgctgaag catcagctgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gagtatggcc agttgctgtt cgcgagagcg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gctacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatataa cagcgccggt tttcagcgag
    16741 agcaatgcgc atcaaagcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga gcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctacgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaata aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtctagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag tcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgctta cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgtac aacaagatgt tgggtgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gcagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt aacgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgcccaggc gggtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcaa ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc cttcaataag cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ttgcacttta tctaaccata gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagtaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacgatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gagtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg ggtaat
//
LOCUS       DADWTH010000073        18261 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.073, whole genome shotgun sequence.
ACCESSION   DADWTH010000073 DADWTH010000000
VERSION     DADWTH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.073"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="JNY02_002718"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="JNY02_002718"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBB9434203.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="JNY02_002719"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="JNY02_002719"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBB9434204.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="JNY02_002720"
     CDS             complement(1729..3000)
                     /locus_tag="JNY02_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9434205.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4387)
                     /locus_tag="JNY02_002721"
                     /pseudo
     CDS             complement(3183..4387)
                     /locus_tag="JNY02_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4487..4843)
                     /locus_tag="JNY02_002722"
                     /pseudo
     CDS             complement(4487..4843)
                     /locus_tag="JNY02_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="JNY02_002723"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JNY02_002723"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JNY02_002724"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JNY02_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBB9434206.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="JNY02_002725"
     CDS             6773..9829
                     /locus_tag="JNY02_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBB9434207.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="JNY02_002726"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="JNY02_002726"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBB9434208.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="JNY02_002727"
     CDS             10336..10524
                     /locus_tag="JNY02_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBB9434209.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="JNY02_002728"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="JNY02_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBB9434210.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="JNY02_002729"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="JNY02_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBB9434211.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="JNY02_002730"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="JNY02_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBB9434212.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="JNY02_002731"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="JNY02_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBB9434213.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTVHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGFKQQVLARIAQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="JNY02_002732"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="JNY02_002732"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBB9434214.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="JNY02_002733"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="JNY02_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBB9434215.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="JNY02_002734"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="JNY02_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBB9434216.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="JNY02_002735"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="JNY02_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBB9434217.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4804 a         4405 c         4690 g         4362 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccagcca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcttgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgaacg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagctatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc
     4141 atatagcagc acattccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgaca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaataaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttactgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcagcg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg gacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgtccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gctagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggcctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gcatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaaatcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac tttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagtt atcgcggcca ggaaacgcac gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gtccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gtttaaacag caggtgctgg cccgaatcgc tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg gttgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca gcgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcggcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt g
//
LOCUS       DADWTH010000074        18173 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.074, whole genome shotgun sequence.
ACCESSION   DADWTH010000074 DADWTH010000000
VERSION     DADWTH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.074"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..2083
                     /gene="pbpC"
                     /locus_tag="JNY02_002736"
                     /pseudo
     CDS             1..2083
                     /gene="pbpC"
                     /locus_tag="JNY02_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2233..2664
                     /gene="ndk"
                     /locus_tag="JNY02_002737"
     CDS             2233..2664
                     /gene="ndk"
                     /locus_tag="JNY02_002737"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBB9434218.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2814..3968
                     /gene="trmG/rlmN"
                     /locus_tag="JNY02_002738"
     CDS             2814..3968
                     /gene="trmG/rlmN"
                     /locus_tag="JNY02_002738"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBB9434219.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4253..5266
                     /gene="rodZ"
                     /locus_tag="JNY02_002739"
     CDS             4253..5266
                     /gene="rodZ"
                     /locus_tag="JNY02_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBB9434220.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5293..6411
                     /gene="ispG"
                     /locus_tag="JNY02_002740"
                     /gene_synonym="gcpE"
     CDS             5293..6411
                     /gene="ispG"
                     /locus_tag="JNY02_002740"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBB9434221.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6522..7796
                     /gene="hisS"
                     /locus_tag="JNY02_002741"
     CDS             6522..7796
                     /gene="hisS"
                     /locus_tag="JNY02_002741"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBB9434222.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7814..8434
                     /gene="yfgM"
                     /locus_tag="JNY02_002742"
     CDS             7814..8434
                     /gene="yfgM"
                     /locus_tag="JNY02_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBB9434223.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8445..9623
                     /gene="bamB"
                     /locus_tag="JNY02_002743"
     CDS             8445..9623
                     /gene="bamB"
                     /locus_tag="JNY02_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBB9434224.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9741..11213
                     /gene="der"
                     /locus_tag="JNY02_002744"
     CDS             9741..11213
                     /gene="der"
                     /locus_tag="JNY02_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBB9434225.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11283..11498
                     /locus_tag="JNY02_002745"
     CDS             11283..11498
                     /locus_tag="JNY02_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBB9434226.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="JNY02_002746"
     CDS             complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="JNY02_002746"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBB9434227.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13027..14493
                     /gene="guaB"
                     /locus_tag="JNY02_002747"
     CDS             13027..14493
                     /gene="guaB"
                     /locus_tag="JNY02_002747"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBB9434228.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14562..16139
                     /gene="guaA"
                     /locus_tag="JNY02_002748"
     CDS             14562..16139
                     /gene="guaA"
                     /locus_tag="JNY02_002748"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBB9434229.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKTDLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16234..16773)
                     /locus_tag="JNY02_002749"
     CDS             complement(16234..16773)
                     /locus_tag="JNY02_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBB9434230.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16789..17307)
                     /locus_tag="JNY02_002750"
     CDS             complement(16789..17307)
                     /locus_tag="JNY02_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBB9434231.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17618..17809)
                     /locus_tag="JNY02_002751"
     CDS             complement(17618..17809)
                     /locus_tag="JNY02_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBB9434232.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17827..17979
                     /locus_tag="JNY02_002752"
     CDS             17827..17979
                     /locus_tag="JNY02_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434233.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4299 a         4450 c         4994 g         4430 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg tacggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgctcag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg
      661 cgcagctcac tggcgatgat cgtggttaat cataccgata tgcgtgttcg cggctgggtg
      721 ggatcggttg atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc
      781 cgatcgccag gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg
      841 atccacccgg catcactgct gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt
      901 aactttgata gcggttttca tggcccgatc aacatgagcg aggcgctggt gcgctcgctg
      961 aacttaccag ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta
     1021 cgcaatgttg gattgccgtt atatttgccc aacggcgctg cgccgaatct ttcactcatt
     1081 ctcggcggcg ctggtgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc
     1141 cacggcaagg caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta
     1201 atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg
     1261 ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc
     1321 tatcgtgacg cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc
     1381 agacctgacg gcacgcccgt tgttggtcag tttggctttg ccagtgccgt accattagtt
     1441 gaatcaggtc aataatatct tactgtcgcg cagtgcgaat ctgccggaag acccgcgccc
     1501 gaactcagtc actcgtggcg ttatctgctg gccgggcgga cagtctttgc cggaaggtga
     1561 cggtaactgt cgccgccgcc tggcaacctg gctgctggat ggcagccagc cgccaacgct
     1621 gttactgtca gagcaggaag ggattaatgg cattcgcttc cccatctggc tggatgacaa
     1681 agggcagcgc gtcgccgctg acttcccgca ggcgaagcaa gaaatggtaa atgtctggcc
     1741 gctaccgctg gaaccctggc tgcccgcatc agaacgtcgc gctgtgcgct taccaccggc
     1801 ctcgacaatt tgcccgccat acggtcacga tgcccagctc cctctgcagc tgacaggcgt
     1861 tcgcgatggc gcgattatta aacgtttacc gggcgcagcg gaagcgactt tgccgttgca
     1921 atcaagtgga ggggcaggtg aacgctggtg gtttctgaat ggcgaaccgt taactgaacg
     1981 cgggcgcaac gtgaccctgc atttgacgga taaaggtgat tatcaattgc tggtgatgga
     2041 tgatgtgggg caaatcgcga cagtgaaatt tgtcatgcaa tagtctgttt tgtccctgat
     2101 tgttgctaaa actcatttta ttttaaaaaa aatgttacct tcatcaatag tcaacggccc
     2161 tgttgctcat tataatccgc gccatctcgt acgctggtac agacaacaac agaacaattt
     2221 acagaggtaa aaatggctat tgaacgtact ttttccatca tcaaaccgaa cgcggtagca
     2281 aaaaacgtca ttggtaatat ctttgcgcgc tttgaagctg cagggttcaa aattgtcggc
     2341 accaaaatgc tgcacctgac cgttgaacag gcacgtggct tttatgctga acacgatgga
     2401 aaaccgttct ttgatggtct ggttgaattc atgacctctg gcccgatcgt ggtttccgtg
     2461 ctggaaggtg aaaacgccgt tcagcgtcac cgcgatctgc tgggcgcgac caatccggca
     2521 aacgcactgg ctggtactct gcgcgctgat tacgctgaca gcctgaccga aaacggtacc
     2581 cacggttctg attccgtcga atctgccgct cgcgaaatcg cttatttctt cggcgaaggc
     2641 gaagtgtgcc cgcgcacccg ttaataattt cgtaaatgcc gcgtgcaaac gtggcatccg
     2701 tgcgccagaa tttgtacaat gtagcgcccc cggacgagca gccgctcacc ggggcgtttc
     2761 ttttttcaac cctccagggg ccataacgtg taataacgag gccggaatag catatgtctg
     2821 aacaattagt cacacctgaa aacgtcacca cgaaagatgg aaaaatcaac ctgctggatc
     2881 tcaaccgtca gcagatgcgg gagtttttta aagatttagg tgaaaaaacc ttccgcgccg
     2941 atcaggtgat gaagtggatg tatcactatt gctgcgacaa ctttgatgag atgaccgaca
     3001 tcaacaaagt gttgcgcggc aaactgaaag aggtggcgga aatccgcgca ccggaagtgg
     3061 ttgaagaaca gcgttcatcc gacggcacca ttaaatgggc gatcgccgtt ggcgatcagc
     3121 gcgtcgaaac ggtgtatatc ccggaagacg accgtgccac gctctgcgtc tcttcgcagg
     3181 tggggtgtgc gctggagtgt aaattctgtt ccaccgccca gcagggcttt aaccgcaacc
     3241 tgcgggtatc ggaaattatt ggccaggtgt ggcgtgcggc gaaaatcgtc ggcgcagcaa
     3301 aagtcaccgg tcagcgccca atcactaacg tcgtgatgat gggcatgggc gagccgctgc
     3361 tcaacctgaa caacgtcgtt ccggcgatgg aaatcatgct tgatgatttc ggttttggcc
     3421 tgtctaaacg tcgcgtgacg ctttccactt ccggtgtagt tccggcgctg gataaactgg
     3481 gcgatatgat cgacgttgca ctggcaattt ccctgcacgc gccgaacgac gaaattcgtg
     3541 acgaaattgt gccgatcaac aaaaagtaca atatcgaaac gttccttgct gcggtgcgcc
     3601 gttatctgga gaaatccaac gccaatcagg gccgagtcac tattgaatac gtgatgcttg
     3661 accacgttaa cgacggcact gaacacgcgc accaactggc ggaactgctg aaagatacgc
     3721 cgtgtaagat caacctgatc ccgtggaacc cgttcccggg cgcgccgtat ggacgcagtt
     3781 cgaacagccg tatcgaccgc ttctcaaaag tgctgatgag ctacggtttc accactattg
     3841 tgcgtaaaac gcgtggtgat gatatcgatg ctgcctgtgg tcagttggcg ggcgatgtta
     3901 tcgaccgtac gaaacgtact ctgcgtaaac ggatgcaggg tgaagccatc gatattaaag
     3961 cggtctgata gccattacgc cacggttact ttatgtaccg tggcgtaatc ttttatgaat
     4021 gaagtattaa gttgcaattg tctggtcaat attaacggtg cgtttttgct gactttaagg
     4081 cagtatgtaa cgatgcaaca gtaagttagc ctcagtgaat gtgggctttg tcacgagcac
     4141 acagacggtc ttatactgta tgataacggt taacttaacg gatgtttcgc ggtgtgggtg
     4201 agcatgatgg ttcaccggca tctcaattct catttaaacg tacctgcagc gaatgaatac
     4261 tgaagccacg cacgaccaaa atgaagcact tactaccggc gctcgcctgc gtaatgctcg
     4321 cgaacaacta ggacttagtc agcaggccgt tgccgagcga ctttgcctga aggtttccac
     4381 ggtacgcgac attgaagaag ataaggcacc cgccgatctt gcttcaacat tcctgcgcgg
     4441 atatatccgc tcttatgcgc gtctggtaca tattccagaa gaagaactgc tgccagggct
     4501 ggaaaagcag gctccacttc gggctgcaaa agttgcgcca atgcagagtt tttccctcgg
     4561 taaacgccgc aaaaaacgcg acggctggct gatgaccttc acctggctgg tgttgtttgt
     4621 ggttatcggc ctgagcggtg cctggtggtg gcaagaccac aaagctcagc aggaagagat
     4681 caccactatg gccgatcaat cttcggcgga actgagcagt aatagcgagc aggggcagag
     4741 tgttccgtta aatacgtcga caactacaga cccggctaca acgtccacgc cgccagcgtc
     4801 tgtggatact accgcaacca acacgcaaac acctgccgta actgcgccag caccagctgt
     4861 tgatccgcaa cagaatgcgg ttgtttcgcc ttcgcaggca aatgttgata ccgccgcgac
     4921 cccggtacca acggcagcaa caacgccaga tggtgctgcg ccgttgccaa ccgatcaggc
     4981 tggcgtgacc acgccggcgg ctgatccgaa tgcgctggtg atgaacttta ctgccgattg
     5041 ctggctggag gtcactgatg ctaccggtaa aaaattgttt agcgggatgc agcgtaaaga
     5101 cggtaatttg aatttaaccg gccaggcacc gtataaactg aaaattggtg cgccagccgc
     5161 agtacagatc cagtatcaag ggaaacctgt cgatctgagt cgttttatca gaactaacca
     5221 ggttgcgcgt ctgaccctca atgccgaaca atcaccggcg cagtaacaga cgggtaacgc
     5281 gggagatttt tcatgcataa ccaggctcca attcaacgta gaaaatcaac acgtatttac
     5341 gttgggaatg tgccgattgg cgatggtgct cccatcgccg tacagtccat gaccaatacg
     5401 cgtacgacag atgtcgaagc aacggtcaat caaatcaagg cgctggaacg cgttggcgct
     5461 gatatcgtcc gtgtctccgt accgacgatg gacgcggcag aagcgttcaa actcatcaaa
     5521 cagcgggtta acgtgccgct ggtggctgac atccacttcg actatcgcat tgcgctgaaa
     5581 gtagcggaat acggcgtcga ttgtctgcgt attaaccctg gtaatatcgg taatgaagag
     5641 cgtattcgca tggtggttga ctgtgcgcgc gataaaaaca ttccgatccg tattggcgtt
     5701 aacgccggat cgctggaaaa agatctgcaa gaaaagtatg gcgaaccgac gccgcaggcg
     5761 ttgctggaat ccgccatgcg tcatgttgat catctcgatc gcctgaactt cgatcagttc
     5821 aaagtcagcg tgaaagcgtc tgacgtcttc ctcgctgttg agtcttatcg tttgctggca
     5881 aaacagatcg atcagccgct gcatctgggg atcaccgaag ccggtggtgc acgcagcggg
     5941 gcagtaaaat ccgccattgg tttaggtctg ctgctgtcgg aaggcatcgg cgacacgctg
     6001 cgcgtatcgc tggcggccga tccggtcgaa gagatcaaag tcggtttcga tattttgaaa
     6061 tcgctgcgta tccgttcgcg agggattaac ttcatcgcct gcccgacctg ttcgcgtcag
     6121 gaatttgatg ttatcggtac ggttaacgcg ctggagcaac gcctggaaga tatcatcact
     6181 ccgatggacg tttcgattat cggctgcgtg gtgaatggcc caggtgaggc gctggtttct
     6241 acactcggcg tcaccggcgg caacaagaaa agcggcctct atgaagatgg cgtgcgcaaa
     6301 gaccgtctgg acaacaacga tatgatcgac cagctggaag cacgcattcg tgcgaaagcc
     6361 agtcagctgg acgaagcgcg tcgaattgac gttcagcagg ttgaaaaata ataacgtgat
     6421 gggaagcgcc tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa cattgaggga
     6481 agcgttgagg gttcattttt atattcagaa agagaataaa cgtggcaaaa aacattcaag
     6541 ccattcgcgg catgaacgat tacctgcctg gcgaaacggc catctggcag cgcattgaag
     6601 gcacactgaa aaacgtgctc ggcagctacg gttacagtga aatccgcttg ccgattgtag
     6661 agcagacccc gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga
     6721 tgtacacctt tgaggatcgc aatggcgaca gcctgactct gcgccctgaa gggacggcgg
     6781 gctgtgtacg cgccggcatc gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt
     6841 atatcgggcc gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc
     6901 agttgggctg cgaagttttc ggtctgcaag gtccggatat cgacgctgaa ctgattatgc
     6961 tcactgcccg ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt gagctgaatt
     7021 ctatcggttc gctggaagca cgcgccaatt accgcgatgc gctggtggca ttccttgagc
     7081 agcataaaga aaagctggac gaagactgca aacgccgcat gtacactaac ccgctgcgcg
     7141 tgctggattc aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg
     7201 attatctgga cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg
     7261 cggggatcgc ttacaccgta aaccagcgtc tggtgcgtgg tctggattac tataaccgta
     7321 ccgttttcga gtgggtgact aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc
     7381 gttatgacgg tcttgtggaa caactgggcg gtcgtgcaac accggctgtc ggttttgcga
     7441 tgggcctcga acgtcttgta ttgttagtac aggccgttaa tccggaattt aaagccgatc
     7501 ctgttgtcga tatatacctg gtggcttcag gtgctgatac acaatctgcg gctatggcat
     7561 tagctgagcg tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac cacggcggcg
     7621 gcaactttaa gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc
     7681 tgggtgagtc tgaagtggct aacggcacag cagtagtgaa ggatttgcgc tctggtgagc
     7741 aaacggcagt tgcgcaggat agcgtagccg cgcatttgcg cacgttactg ggttaaggaa
     7801 ggagaaggac agcgtggaaa tttacgagaa cgaaaacgac caggtagaag cggttaaacg
     7861 cttttttgct gaaaatggca aagcactggc tgttggggtg attttgggcg ttggcgcact
     7921 gattggctgg cgctactgga acagccatca ggttgattct gcacgctccg cttctcttgc
     7981 ctatcaaaat gcggttaccg cagtgagcga aggcaaaccg gatagcatcc cggcggcgga
     8041 aaaatttgct gctgaaaata aaaatactta tggtgcgctg gcttctttgg aacttgcgca
     8101 gcaatttgtt gacaaaaatg aactagagaa agctgccgcc cagttacaac aggggctggc
     8161 agacacgagc gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt
     8221 acagctcaag caggctgatg ccgcgctgaa aacccttgat accatcaaag gtgaagggtg
     8281 ggctgccatt gttgccgacc tgcgtggtga agcattgctg agcaaaggtg ataagcaagg
     8341 tgcgcgtagt gcatgggaag caggcgtgaa aagcgatgtt actccggcac tgagcgaaat
     8401 gatgcagatg aaaattaata atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat
     8461 tactgctgcc aggactgctt tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg
     8521 aagaagatgt ggtaaagatg tccccattgc caaccgttga aaaccagttt acgccgacca
     8581 cggcgtggag cacttccgtt ggtagcggca ttggcaactt ctattccaat cttcatccgg
     8641 cactggcgga caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg
     8701 cggatgatgg caaagaaatc tggtctgtca gcctggccga gaaagatggc tggttctcta
     8761 aagagcctgc attactttct ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca
     8821 gcgaaaaggc gcaggtttac gcgctgaata ccagcgatgg tactgtggca tggcaaacta
     8881 cagtcgcggg tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca
     8941 ccagtaacgg tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta
     9001 acctcgatat gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg
     9061 cggccgtcgt gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa cagggccaga
     9121 tgatttggca gcagcgtatt tcccaggcga ccggttctac cgaaattgac cgtctgagcg
     9181 atgttgacac gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc tataatggta
     9241 acctgacggc gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg
     9301 tgaatgattt catcgtcgat ggcaatcgca tctatctggt cgatcaaaat gaccgggtga
     9361 tggcgttgac cattgatggc ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc
     9421 tgctgacttc tccggtgctg tataatggca acctggtggt cggtgacagt gaaggttatc
     9481 tgcactggat taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg
     9541 gtttccagac tgaaccggtt gccgctgacg gcaaactgct gatccaggca aaagacggaa
     9601 ccgtgtactc tattacacgt taatcgtctc tgtcgttcac tttgaaaacg gctcctggac
     9661 aggggccgtt ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt ctgatgattt
     9721 ataaaaatga ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag
     9781 gaaaatccac gttatttaac cgtctaactc gcacccgaga tgcgctggtt gcggatttcc
     9841 cgggtctgac tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt gagtttatct
     9901 gtattgatac cggcgggatt gatggcacag aagacggtgt agaaacccgc atggcggaac
     9961 agtcgctgct ggcgattgaa gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg
    10021 gcctgatgcc ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct
    10081 tcctggtggc aaacaaaact gacggtctgg atcccgatca ggcagtggtt gatttctact
    10141 cgcttggttt aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc
    10201 tgctggagca tgtgctgctg ccgtggatgg aagatctcgc accgcaagag gaagtcgacg
    10261 aagacgctga atactgggcg caatttgaag cggaagagaa cggcgaagaa gaagaggaag
    10321 acgacttcga cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag
    10381 gtaagtctac actcactaac cgtattcttg gagaagagcg cgttgttgtt tacgacatgc
    10441 ctggcacgac gcgtgacagc atctacatcc caatggaacg cgatggacgt gagtatgtgc
    10501 tcattgacac cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct
    10561 ccgtaatcaa aacgttgcag gccattgaag acgccaacgt ggtgatgtta gtgattgatg
    10621 cgcgcgaagg tatttccgat caggatctct cgctgctggg ctttattctc aatagtgggc
    10681 gctcacttgt cattgtggtg aataagtggg atggcctgag ccaggaagtg aaagagcagg
    10741 tgaaagaaac gctggacttc cgtctgggct ttatcgattt tgctcgtgtg cactttatct
    10801 ctgccttgca cggcagtggt gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca
    10861 gctccacccg tcgtgtgggg acctctatgc tgacgcgcat catgacgatg gctgttgaag
    10921 atcaccaacc gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg
    10981 gttataaccc gccgattgtg gtgattcacg gtaatcaggt gaaagacctg cctgattcct
    11041 acaagcgcta cttgatgaac tacttccgca aatcgctgga cgtaatggga tcgccgattc
    11101 gtattcagtt caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa
    11161 cccagatgcg taaacgtaag cgtctgatga agcacatcaa gaaaaataaa taattatccg
    11221 taagtatttt atctgccatt ccctctatga atgtagaggg aatggctacc ggaggaattc
    11281 atatggaact gcattgtcca aaatgtcagc atgtgcttga tcaggataac ggccatgcgc
    11341 gttgcccatc ctgcggggaa tttattgaaa tgaaagctct ttgtccagac tgccatcaac
    11401 cgttgcaggt gttaaaagcc tgcggtgcag tagattactt ctgtcagcac gggcatggct
    11461 tgatatcgaa aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta cgcgattttt
    11521 ttaccggctg gatgtttttt acttcacttt ctatccagcc gtcttccaga cgtgtggtta
    11581 gcatttcacc cgctttaact tgcttcactt ttttcagtac attgccgtca gtagcagtag
    11641 taacgctgta tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg tgcgttactg
    11701 cattaccgaa acgttcacgc gtggcgctaa gctgtgcgcg caggatttct gctaaacgat
    11761 attccagttg ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat
    11821 tcagccgctg tgttaatcgc tgctgctgtt gcccggcacg cttaagctga ttttccagcg
    11881 caaagctcat ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc agccggagct
    11941 gcggatgctg ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgcgta cggttggcga
    12001 gataataatc catcgccatc tccagccgtt gacgggccga ttgcacctgg cgcagtaact
    12061 cttgctgatt acggctcact acttcagcgg cggcagacgg cgttggcgca cgcagatcgg
    12121 caacaaaatc ggcaatggtc acatccgtct catgcccgac ggcgctgacg accggaatgc
    12181 ggctggcaaa aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa tcttccagcg
    12241 aaccgccgcc gcgcccaacg atcaacacgt cgcactcatt gcgctgattc gccagttcaa
    12301 tggcgcgaac aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca
    12361 ccggtagaga aggatcgcga cgttttaaca catgcaaaat atcatgtagc gcagcaccgg
    12421 tttttgaggt gatcacacca acgcaatgcg caggggaggg aagtggtttt ttgtattgct
    12481 gatcgaacaa accttcagcc tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca
    12541 gcccttcacc ggccggctgc atactctcaa cgattatctg gtagtcgccg cgcggctcgt
    12601 agagcgtaat attggcgcga actaaaactt gttgcccatg ctgcggacgg aaggtgaccc
    12661 ggcggttgct gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa
    12721 agtaccagtg accggaagcc ggttgtgtga aattagagat ttcgccgctg atccaaacct
    12781 gtcccatctc atgctcaagc agcagacgaa ccgtttgatt caggcgacta acggtaaaaa
    12841 ttgcagggga ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca gcaggttatt
    12901 cagtcgatag taacccgccc ttcggggata gcaagcattt tttgcaaaaa ggggtagatg
    12961 caatcggtta cgctctgtat aatgccgcgg caatatttat taaccactct ggtcgagata
    13021 ttgcccatgc tacgtatcgc taaagaagct ctgacgtttg acgacgttct cctcgttcct
    13081 gctcattcta ccgttctgcc gaatactgct gacctcagca cccagctgac gaaaactatt
    13141 cgtctgaata tccctatgct ttccgcagca atggataccg taacggaagc gcgcctggct
    13201 attgctctgg ctcaggaagg cggtatcggc tttatccaca aaaacatgtc cattgaacgc
    13261 caggcagaag aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag
    13321 actgttctgc caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt
    13381 gcgggctatc cggtcgttac cgaagaaaac gaactggtgg gtattatcac cggtcgtgac
    13441 gtgcgttttg ttaccgacct gaaccagccg gttagcgttt acatgacgcc gaaagagcgt
    13501 ctggtcaccg tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa
    13561 cgcgttgaaa aagcgctggt ggttgatgac gaattccacc tgatcggcat gatcaccgtg
    13621 aaagacttcc agaaagcgga acgtaaaccg aacgcctgta aagacgagca aggccgtctg
    13681 cgtgttggtg ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt
    13741 gccgctggcg ttgacgttct gctgatcgac tcctcccacg gtcactcaga aggcgttttg
    13801 cagcgtatcc gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg
    13861 gcaacagctg caggtgcacg cgctttggca gaagctggtt gcagcgcggt taaagtcggt
    13921 atcggccctg gttctatctg tacgactcgt atcgttactg gcgtaggtgt tccgcagatc
    13981 actgccgttg ctgacgcagt tgaagccctg gaaggcaccg gtattccggt tatcgcagat
    14041 ggtggtattc gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg
    14101 atggtaggtt ccatgctggc gggtactgaa gaatctccgg gtgaaatcga actctaccag
    14161 ggccgttctt ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc
    14221 tctgaccgtt atttccagag cgataacgct gccgacaaac tggtgccgga aggtatcgaa
    14281 ggtcgcgtag cttataaagg tcgcctgaaa gagatcattc accagcagat gggcggcctg
    14341 cgctcctgta tgggtctgac cggctgtggt actatcgacg aactgcgtac taaagcggag
    14401 tttgtacgta tcagcggtgc gggcattcag gaaagccacg ttcacgacgt gaccattact
    14461 aaagagtccc cgaactaccg tctgggctcc tgattctctt cgcccgactt catgtcgggc
    14521 gatttatatt atctgtttca cttgcctcgg aataagcgtc aatgacggaa aacattcata
    14581 agcatcgcat cctcattctg gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg
    14641 tgcgtgagct gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc
    14701 gtgacttcaa tccaagcggc attattcttt ccggcggccc ggaaagtact actgaagaaa
    14761 acagtccgcg tgcgccgcag tatgtctttg aagcaggcgt accgatattc ggcgtttgct
    14821 atggcatgca gaccatggca atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg
    14881 aatttggcta cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag
    14941 atgcgctgac cgcagacggt aaaccgctgc tcgatgtctg gatgagccac ggcgataaag
    15001 ttaccgctat cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca
    15061 ttatggctaa cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa gtgactcaca
    15121 cccgccaggg tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc
    15181 tgtggacgcc agcgaaaatt atcgacgatg ctgtagctcg catccgcgag caggtaggcg
    15241 acgataaagt catcctcggc ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc
    15301 tgcaccgtgc tatcggtaaa aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc
    15361 tcaatgaagc agagcaggtt ctggatatgt ttggcgatca ctttggtctt aacattgttc
    15421 acgtaccggc agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa
    15481 aacgtaaaat catcgggcgc gttttcgttg aagtattcga tgaagaagcg cagaaactgg
    15541 aagacgtgaa gtggctggcg cagggcacca tctaccctga cgttatcgaa tctgcggctt
    15601 ctgcaaccgg taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag
    15661 agatgaagat gggcctggtt gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga
    15721 ttggtctgga gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag
    15781 gccttggcgt tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg
    15841 ctgacgccat cttcattgaa gaactgcgta aaacggacct gtacgacaaa gtcagccagg
    15901 cgttcaccgt cttcctgcca gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg
    15961 actgggttgt ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc
    16021 atctgccgta cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta
    16081 tttcccgcgt ggtgtatgac atcagcggca agccgccagc taccattgag tgggaatgat
    16141 tttgatccct gcactatgaa tgaacaaaac cctccgttac tacagagggt tttttatctt
    16201 caagaatcat aggcttgaag ttactaacat cgattaatta aaccagctgt ccgatttgtt
    16261 ctcttctgct ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca tatttctgtt
    16321 tgcgtcagct tcagattgca ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc
    16381 gataaattcg ttttcgcgct taacgcgggc ttcttctttc gccagcgcca gtttttgttt
    16441 ctgaatctct aagctgcgta gctcatcttc ataactttga tcgcgttttt tgtccgcagt
    16501 ggcttcggcg tccagtttat cctgacgagc tttcttattt gccgctgccg ttgccgctct
    16561 tttgttcgcg gcggcctggg catttgcgcg acgttgcttc tcttgctgga tttccctgtt
    16621 gcgctccgcg acccattcgt catgctgcct ttgctcttca tttttacctt gctgttccgc
    16681 ttctgcgaca gcagagagtt gatcctgcaa tgatgaggct atagccggat agcttaagga
    16741 ggctaagatg gcgcaaagaa aaactttctt catgactcct cctgattatt agctcttttc
    16801 aggacatttg gtatttggct gaatacgcgt ttcgttatac gttgtggtaa taacaacggc
    16861 taaacctgtc gtaaactggc actctttacc ctcctgggtg gaggtataca ctttggtgcc
    16921 ttccttatat gttaaagaaa caccttccac taaggtttta tcattcacca tagaacccgc
    16981 tgccgcgcct acagctccgc cgccaactgc ccctgccgtc gttccggaat tgctgccaga
    17041 accgacgttg tggccgataa caccgccagc gactgcgcca ataagcgcgc cgaaggcttg
    17101 tgcgttccgt ttattttggg agttgtctac ggcaactttt gcgggaagaa tggaaataat
    17161 attaacggtt ttagtttctt gtttggtatt cagttgatcg gtttgataaa catcggcggc
    17221 atgatcgtca gcatttgact ggcatcctgc cagagtgaat gacgctaaca ttgccacagg
    17281 cagaagacat tttttaaatt tcatcactat tccttgttat acacattacg aaaaataaga
    17341 catttaagat catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat agattaaatt
    17401 tattaaaatt cacgcaggga ataatttata aaaatatatc attacaacat atatattaat
    17461 tttatattta tttcgcagcc aaacaggata aacatcagtc aggacaaaga cgtaaaattt
    17521 aagatgaaag aatatctgcc gctttgataa tcccaaagag caaaagcatc cgacagcgaa
    17581 gtgccgttat gttctgtgaa agtggagaag gtaaccgcta acgttgtacc ggtgattcga
    17641 cggagagtgt ggattgctga gcttcttttt tgctctgatg gtgcagccag gcaccgacgg
    17701 acgagtagat aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca
    17761 tgcgactgtt aaatcggtgt cgttttcgca tactagttgc ctgactcaca aaaggttcct
    17821 tgaagtatgt cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc
    17881 aattcttcgt tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat
    17941 ttaattattt acaatgatga tgtaataatg atgaattaac atgataaacg taataattca
    18001 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
    18061 caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
    18121 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atg
//
LOCUS       DADWTH010000075        17649 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.075, whole genome shotgun sequence.
ACCESSION   DADWTH010000075 DADWTH010000000
VERSION     DADWTH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.075"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            144..473
                     /locus_tag="JNY02_002753"
     CDS             144..473
                     /locus_tag="JNY02_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBB9434234.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            721..966
                     /gene="nrdH"
                     /locus_tag="JNY02_002754"
     CDS             721..966
                     /gene="nrdH"
                     /locus_tag="JNY02_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBB9434235.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            963..1373
                     /gene="nrdI"
                     /locus_tag="JNY02_002755"
     CDS             963..1373
                     /gene="nrdI"
                     /locus_tag="JNY02_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBB9434236.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1346..3490
                     /gene="nrdE"
                     /locus_tag="JNY02_002756"
     CDS             1346..3490
                     /gene="nrdE"
                     /locus_tag="JNY02_002756"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBB9434237.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLQEAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3500..4459
                     /gene="nrdF"
                     /locus_tag="JNY02_002757"
     CDS             3500..4459
                     /gene="nrdF"
                     /locus_tag="JNY02_002757"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBB9434238.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            4814..6016
                     /gene="proV"
                     /locus_tag="JNY02_002758"
     CDS             4814..6016
                     /gene="proV"
                     /locus_tag="JNY02_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBB9434239.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6009..7073
                     /gene="proW"
                     /locus_tag="JNY02_002759"
     CDS             6009..7073
                     /gene="proW"
                     /locus_tag="JNY02_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBB9434240.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7131..8123
                     /gene="proX"
                     /locus_tag="JNY02_002760"
     CDS             7131..8123
                     /gene="proX"
                     /locus_tag="JNY02_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBB9434241.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8315..9494
                     /locus_tag="JNY02_002761"
                     /pseudo
     CDS             8315..9494
                     /locus_tag="JNY02_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9618..10355
                     /gene="ygaZ"
                     /locus_tag="JNY02_002762"
     CDS             9618..10355
                     /gene="ygaZ"
                     /locus_tag="JNY02_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBB9434242.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10345..10680
                     /gene="ygaH"
                     /locus_tag="JNY02_002763"
     CDS             10345..10680
                     /gene="ygaH"
                     /locus_tag="JNY02_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBB9434243.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            10771..11301
                     /gene="emrR"
                     /locus_tag="JNY02_002764"
     CDS             10771..11301
                     /gene="emrR"
                     /locus_tag="JNY02_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBB9434244.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            11428..12600
                     /gene="emrA"
                     /locus_tag="JNY02_002765"
                     /pseudo
     CDS             11428..12600
                     /gene="emrA"
                     /locus_tag="JNY02_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            12617..14155
                     /gene="emrB"
                     /locus_tag="JNY02_002766"
     CDS             12617..14155
                     /gene="emrB"
                     /locus_tag="JNY02_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBB9434245.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="JNY02_002767"
     CDS             complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="JNY02_002767"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBB9434246.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="JNY02_002768"
     CDS             complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="JNY02_002768"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBB9434247.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(16513..16941)
                     /locus_tag="JNY02_002769"
     CDS             complement(16513..16941)
                     /locus_tag="JNY02_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBB9434248.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="JNY02_002770"
     CDS             complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="JNY02_002770"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBB9434249.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVHPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4181 a         4592 c         4497 g         4379 t
ORIGIN      
        1 ctcgttattt tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
       61 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
      121 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
      181 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
      241 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcgt
      301 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
      361 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
      421 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa
      481 atgataataa atacgcgtct ttgaccccga agcctgtctt cggggtttct ttttgcctgg
      541 tgaatcacaa aaatccccct cccccgtcac gctcatatcc agggtaattt cgatcactat
      601 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
      661 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacaga aatacgaatc
      721 atgcgcataa ctatttatac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
      781 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg
      841 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
      901 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
      961 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
     1021 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     1081 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     1141 gacaggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
     1201 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     1261 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
     1321 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
     1381 aacgatggat taccacgcgc tgaatgcgat gcttaatctt tacgatagcg caggtcgcat
     1441 tcagttcgat aaagaccgcc aggccgttga cgcctttatt gcgacgcatg tgcgtccgaa
     1501 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
     1561 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
     1621 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
     1681 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat
     1741 ggtggcgctg acgctggcac aaggcgatga gacgctggcg ttgcaactga ccgatgaaat
     1801 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
     1861 cggcgaactg gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg
     1921 ggcggtaaat tccgcactgc agctgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
     1981 gaatctgcag gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
     2041 tccggtgatg aaaatgttgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     2101 gggggcaggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac
     2161 gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     2221 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     2281 tgacgttgag cgagtttatg gcaagccgtt tgccgatatc gccattagcg aacactatga
     2341 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     2401 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacagt
     2461 aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt
     2521 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     2581 tatttcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgatttttc
     2641 ccgcacggta gagactgccg tgcgcggttt aacggcggtg tcagatatga gtcatatccg
     2701 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
     2761 gaatttacat ggttatctgg cgagggaagg catcgcttat ggttcgccgg aagccctgga
     2821 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     2881 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
     2941 atatttcagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     3001 gtttgcccgc agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga
     3061 cgtgatgcgc tacggcatat acaaccagaa tctccaggcg gtgccgccaa ccgggtctat
     3121 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     3181 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     3241 ggcgctgtat caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga
     3301 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     3361 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta
     3421 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     3481 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     3541 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     3601 aaaggtgccg ctgtcgaacg atattcccgc ctggcagaca ttaactgtcg tagaacaaca
     3661 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     3721 cgcgccttcg ctgatgcccg atgcgctcac gcctcatgaa gaggcggtat tatcgaatat
     3781 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     3841 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     3901 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
     3961 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     4021 ccgcggaaag ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt
     4081 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
     4141 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt
     4201 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     4261 ctgttacaac accaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga
     4321 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     4381 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
     4441 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc agcagggaaa
     4501 taattcccgc caaatagctt tttatcacgc aaatattctg tagtgatcta cactgatact
     4561 ctgttgcatt attcgcctga aactacaata ttcaggcgtt ttttcgctat ctatgacaaa
     4621 aaatatcaac ttttctcgat ttgctctcag cccttatatc acggaaaatt ccggcgattt
     4681 gctcgcatca atattcatgc cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt
     4741 tagggtagaa aaaagtgact attcccattg ggtaatatat cgacataaac aaataaagga
     4801 atcattctat tgcatggcca ttaaattaga aattaaaaat ctttataaaa tatttggtga
     4861 gcatccacag cgagcgttca aatatatcga acaaggactt tcaaaagaac aaattctgga
     4921 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     4981 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     5041 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     5101 cgacgccgaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     5161 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     5221 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     5281 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     5341 ccgcgcgtta gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga
     5401 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     5461 caccattgtc tttatttccc acgatcttga tgaagccatg cgtattggcg accgaattgc
     5521 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     5581 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tgttcagtgc
     5641 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaactcctg gcttcggccc
     5701 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     5761 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     5821 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     5881 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     5941 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     6001 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     6061 cgcaatccgc agacgcctgg gggacaccga cgactgcacc ggctgacggc ggtggtgctg
     6121 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     6181 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     6241 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc
     6301 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     6361 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ctcgctgatt gccatcggcg
     6421 caatcggtgc ctgttcgcag gcaatggtga ctctggcgct ggtgttaact gccctgctgt
     6481 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     6541 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc
     6601 caatcgtcat gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg
     6661 cgctgccgcc gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga
     6721 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     6781 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta
     6841 tggtggtcat tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     6901 tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     6961 ttatcctcga tcgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     7021 gctggtacac cactggccct gttggtctgc tgacccgccc attcattaag taactctgca
     7081 cttgcccggt gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     7141 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc
     7201 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     7261 agacgctgct ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg
     7321 aagtggatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     7381 tgaactggac gccactccat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     7441 atcgtgaagg ggtatttgtt aacggcgcag cacagggtta cctgatcgat aagaaaaccg
     7501 ccgaccaata caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     7561 tcgataccaa cggcgacggt aaagcggatt taaccggttg taaccctggc tggggctgcg
     7621 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     7681 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     7741 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     7801 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaatgcc gataccaaac
     7861 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     7921 cctggaccga gaaaaatccg gcggcagcca aactgtttgc cattatgcag ttgccagtgg
     7981 ccgatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     8041 agggccacgt tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     8101 aggcgctggc agcgcagaag taatttttat tcgggcggat aaggcgtttt cgcctcatcc
     8161 gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc ctatcatgcc tacaaaccac
     8221 atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc tattattgat
     8281 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
     8341 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca gtaactatta
     8401 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     8461 ctttattgtt accgccgcgc agttgggcta tgccgcaggt ctactgtttc ttgttcccct
     8521 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     8581 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     8641 cggtttattc tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     8701 ggataaacgc ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct
     8761 ggcacgaacc gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt
     8821 tgcttcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
     8881 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagca tgttcatcag
     8941 cgataagatc ctgcgcaccc gcgcgttgct gggctgcatg acctttgcca acttcagcat
     9001 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtga
     9061 cattggtctg tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
     9121 ttttgccgac aagggcaaat cgcaccacaa ctttcggtct gcttctgctg ttactttcat
     9181 ggctggcaat ctggtttggt cacacttccg tactggcgtt gattgtcggc attctggtgc
     9241 tggacctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacatc
     9301 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
     9361 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
     9421 ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt catcgtcagg
     9481 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
     9541 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
     9601 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
     9661 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg gtggcctttg
     9721 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
     9781 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gccgggagta
     9841 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt ttgtatggcc
     9901 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctatgggcgt
     9961 ttggcctgac ggatgaggtt tttgccgccg ccaccgcaaa actggtacgc aataatcgcc
    10021 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    10081 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    10141 ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tctttccagc
    10201 gcaaacaatc tctttgcgtt accgcagctt tagttggtgc ccttgcaggc gtaacgctat
    10261 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    10321 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    10381 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    10441 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    10501 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    10561 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    10621 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    10681 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    10741 atcgactgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    10801 atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat ccttctgact
    10861 cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    10921 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    10981 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    11041 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    11101 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    11161 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagatcag
    11221 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    11281 gttctcgaag cgatgagcta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    11341 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    11401 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    11461 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    11521 ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca tttcgaagaa
    11581 accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt gtctggcagc
    11641 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgatgt tctggtcact
    11701 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    11761 gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa tattgaggtg
    11821 caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    11881 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac cagtgcccag
    11941 gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat tctggggact
    12001 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctagctg
    12061 gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtttcccg tcgtgccgtg
    12121 caaccgggtg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    12181 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgcat cggtcagccg
    12241 gtcaccatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    12301 ctggatatgg gcacaggtag cgcgttctca ctgcttccgg cgcaaaatgc gaccggtaac
    12361 tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa acagctggag
    12421 caatacccgc tgcgcattgg tttgtccacg ctggtaagcg tcaataccac taaccgtgac
    12481 ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac cgcgcgtgaa
    12541 atcagcctag cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    12601 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    12661 gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca ttgctaacgt
    12721 ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccaaggaa cgtgggtaat
    12781 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    12841 cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg cgtcgtgggc
    12901 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    12961 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc cgccagccaa
    13021 acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    13081 gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    13141 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    13201 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    13261 cctgcagatt atgctcgacc gcggtaaaga gctggactgt ttttcatcac aggaaattat
    13321 cattcttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg agctgaccga
    13381 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgctt
    13441 gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc cgcagttgtt
    13501 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    13561 tattccggtg attctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcggcg
    13621 gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    13681 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    13741 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    13801 ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta tcggcacgtc
    13861 gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    13921 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    13981 gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    14041 ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc tggggctggt
    14101 gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggcggtgcgc actaagtaca
    14161 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    14221 agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg ctgttgatgc
    14281 gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    14341 ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc tgcactttca
    14401 gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct ggcgtaccaa
    14461 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    14521 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggatcc
    14581 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    14641 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    14701 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    14761 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    14821 atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc tttttctttt
    14881 ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg tttccatttc
    14941 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    15001 cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc ctgttccgcc
    15061 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    15121 gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt taatgctatc
    15181 cagcgtttgc gcgacgcgtt tcagatcgtg gaacagatct ttacccacct gcagtaacgg
    15241 gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac cttcaaggat
    15301 cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    15361 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    15421 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    15481 taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    15541 atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct taccgtcttt
    15601 ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt taaggcccgc
    15661 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    15721 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    15781 tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa tcgccggaga
    15841 tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga taacgcggaa
    15901 atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc acttcgcttg
    15961 ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt gcatcagcgc
    16021 gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg tgttagaagt
    16081 gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    16141 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    16201 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    16261 atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    16321 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    16381 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    16441 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    16501 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    16561 atagcgtaac gctttaccaa ggcacaaaaa aaagattact ggcccccagg agatgcgcat
    16621 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    16681 gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg atgttttacg
    16741 caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    16801 tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg ccaccagcac
    16861 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    16921 aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc tgctgcacgg
    16981 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg gtgcacgccc
    17041 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatca
    17101 gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa tgcctctgcg
    17161 atggcgcttt cactccccgt tcctacagcc attggacgac gaccatgcca acttttcacc
    17221 acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac tgcttctgtt
    17281 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    17341 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaatatc gtactgaaga
    17401 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    17461 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    17521 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    17581 gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg aaggaaattt
    17641 tttggagaa
//
LOCUS       DADWTH010000076        17572 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.076, whole genome shotgun sequence.
ACCESSION   DADWTH010000076 DADWTH010000000
VERSION     DADWTH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.076"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNY02_002771"
     CDS             complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNY02_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBB9434250.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1337..1903
                     /gene="efp"
                     /locus_tag="JNY02_002772"
     CDS             1337..1903
                     /gene="efp"
                     /locus_tag="JNY02_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBB9434251.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1955..2080
                     /gene="ecnA"
                     /locus_tag="JNY02_002773"
     CDS             1955..2080
                     /gene="ecnA"
                     /locus_tag="JNY02_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBB9434252.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2191..2337
                     /gene="ecnB"
                     /locus_tag="JNY02_002774"
     CDS             2191..2337
                     /gene="ecnB"
                     /locus_tag="JNY02_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBB9434253.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2513..2742
                     /locus_tag="JNY02_002775"
                     /pseudo
     CDS             2513..2742
                     /locus_tag="JNY02_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2739..3272)
                     /gene="blc"
                     /locus_tag="JNY02_002776"
     CDS             complement(2739..3272)
                     /gene="blc"
                     /locus_tag="JNY02_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBB9434254.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3361..4494)
                     /locus_tag="JNY02_002777"
     CDS             complement(3361..4494)
                     /locus_tag="JNY02_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBB9434255.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="JNY02_002778"
     CDS             complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="JNY02_002778"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBB9434256.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="JNY02_002779"
     CDS             complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="JNY02_002779"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBB9434257.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5333..6067)
                     /gene="frdB"
                     /locus_tag="JNY02_002780"
     CDS             complement(5333..6067)
                     /gene="frdB"
                     /locus_tag="JNY02_002780"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBB9434258.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="JNY02_002781"
     CDS             complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="JNY02_002781"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBB9434259.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8193..9170
                     /gene="epmA"
                     /locus_tag="JNY02_002782"
     CDS             8193..9170
                     /gene="epmA"
                     /locus_tag="JNY02_002782"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBB9434260.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9389..10891
                     /gene="yjeM"
                     /locus_tag="JNY02_002783"
     CDS             9389..10891
                     /gene="yjeM"
                     /locus_tag="JNY02_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBB9434261.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="JNY02_002784"
     CDS             complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="JNY02_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBB9434262.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14375..15343)
                     /gene="psd"
                     /locus_tag="JNY02_002785"
     CDS             complement(14375..15343)
                     /gene="psd"
                     /locus_tag="JNY02_002785"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBB9434263.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="JNY02_002786"
     CDS             complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="JNY02_002786"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBB9434264.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16587..17132
                     /gene="orn"
                     /locus_tag="JNY02_002787"
     CDS             16587..17132
                     /gene="orn"
                     /locus_tag="JNY02_002787"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBB9434265.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17343..17418
                     /locus_tag="JNY02_002788"
     tRNA            17343..17418
                     /locus_tag="JNY02_002788"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17376..17378,aa:Gly,seq:gcc)
BASE COUNT         4070 a         4522 c         4613 g         4367 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc aggcaatttt
      241 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
      301 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      361 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      421 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      481 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      541 ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct
      601 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      661 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      721 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      781 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      841 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      901 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      961 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagtggg
     1021 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
     1081 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
     1141 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
     1201 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1261 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1321 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1381 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1441 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1501 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1561 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1621 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1681 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1741 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1801 cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg aagtcatcaa
     1861 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1921 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1981 tttgcttgcc agcacgctgc tcacgggctg taacaccgct tgcggtttcg gcgaagacat
     2041 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     2101 atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     2161 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     2221 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2281 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca
     2341 ataacggtac gacagcagtg tcgtgccgtt tgttttttct gcgatcgtca taaaggtaat
     2401 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2461 cctgcaaacg cctcttttca ccggggacgg ccccaaattt ccggagcctg atatgtcctg
     2521 gattatctta gttattgctg gtctgccgga agtggtatgg gccgttggcc tgaaatatac
     2581 ccacggcttt agccgtttga tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2641 accggcattg tgctgctcgg tgagtccgct aacccgatgc gtctggcgag tctggcgtta
     2701 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2761 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2821 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     2881 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     2941 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3001 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3061 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3121 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3181 actacggtca cgccacgcgg cggcgtagga gaactacagg caacgaccag aaatgcagct
     3241 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3301 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3361 ttactgtaga gcgttgagaa tctgacaggc ggcggcgact ctctctggat tgggatagtt
     3421 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3481 aaatccgccg gtcgcccccg ttttatgtac ccatgatgcg cgtactgcag gagttggggg
     3541 cttaatcgct tttaccgggc gtgctgccag tgcaatttta ttgtcactgc cgttaatgat
     3601 gctgtcagga ttgaccggcc agtccagcat ttcccagccc aggccctgat acatatcgcc
     3661 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagcgttt tctcattgat
     3721 atcaaggggg ggtaaattgc tttgcaccca gcgggccata tcttcaatgg tcgacttcac
     3781 accataagct tcagcatcta acgcccctgg cgaaacatgc actgccttac cttcgcgata
     3841 tccccaggcg taattctttt cttctggggg aggtacatta atccacgtat ggttgagttt
     3901 gagtggctgg aagacacgag tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     3961 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggagc
     4021 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacttcatc
     4081 cggcacctgc aatggcaggc cgccagcggt gtaggtcgca agatgtaata gtgtgatccc
     4141 attccactgt ttagcggtaa gttcaggcca gtattttgtt gcgggatcgc ttaacttgat
     4201 ttcccctcga gcaatagcgt cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4261 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg catagcccca
     4321 ggtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4381 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4441 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acataaggtc
     4501 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatgcgttag
     4561 attgtaacga caccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4621 catttgcccg caggcacgtg gattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4681 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4741 gcgaacgcca gaacgcgctc gtagctcagc gcatcacccg gaaacagccc cagtggcagc
     4801 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4861 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     4921 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     4981 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     5041 cgctttcggt gccagttcaa accaggtttt ggtgtgcagc agagctgccg tcagagtgat
     5101 caggttaatg atcacgataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5161 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5221 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5281 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5341 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgc tgaatggccg
     5401 cagccggatc gacgtgtttc gggcagactt cggagcaata gcccacgaaa gtacagctcc
     5461 atacaccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5521 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5581 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5641 actttgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5701 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5761 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt catactgtcg gtgtaatcac
     5821 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     5881 aaatcgccat acggcaggac cagcggtaac tcaggtccgg tgccaggttg tctttgatgt
     5941 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     6001 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6061 cagccattcg ccttctcctt attattggct gcttccgcct tatcggctgc atccgcttcg
     6121 ccaccgtaaa cgcgtttagc tggcggcagc gtagtaatct tcacgtcgct gtactccagg
     6181 cgagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgagaaagtt gacgtcgtca
     6241 cgctcggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6301 gcggagtgcg ccatacattc agcaacgttc agaccgtggc tcagttcaat ggtgtagagc
     6361 aggtcggtgt tgaacacgct ggaggtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6421 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6481 ccttcttcca ttgccaggcc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6541 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6601 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6661 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6721 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6781 tcgataccgc ccatggtgta gtgtgcagtc ggacgtaccg gaatcggttc tttaaccgga
     6841 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     6901 ttctcgccga ggtgacgcag gtcgagataa accacatcac cacgcggcgt ggagatagtg
     6961 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     7021 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7081 taacggtagc catttttgtt gaccagaata ccgccttcac cacggcagcc ttcggtcatc
     7141 aggataccgg aacctggcag accggttggg tgatactgaa cgaattccat gtcacgcagc
     7201 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7261 ttgtaacgat aaacgcgacc cgcaccgccg gtagccataa cgaccgcgtt agcacggatc
     7321 tgcaccaacg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7381 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7441 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtgcgc
     7501 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7561 gggcatcccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7621 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7681 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7741 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7801 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7861 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     7921 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     7981 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     8041 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8101 tgcccaccgc tgtttgattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8161 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8221 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8281 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8341 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8401 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gctggttgtg
     8461 ggccggtatt ccagctgtgc cgcagcttcc gtaatgaaga gatggggcgt tatcacaacc
     8521 ctgagttcac tatgctggag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8581 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8641 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8701 gggaagtcgc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8761 cgttgctaca attgctgttt acctttggcg tagaggcaaa tattggtaaa gaaaaaccga
     8821 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     8881 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     8941 atgaattaac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     9001 cggcgcgcgg tttgccgcag caccccattg accagaatct gattgaagcc ttgaaagtcg
     9061 gtatgcctga ctgctccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9121 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9181 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9241 ttttccgtta aaggccacaa tttaagactg gatatttgat atcatcaagg tatcaattct
     9301 gtattggttt ttacgctgcc tgctcaatgt tgcgcagtgc agttcagtgg gcacgtgtta
     9361 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9421 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9481 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9541 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat
     9601 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9661 gttttcctct tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc
     9721 aacattcctc tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac
     9781 gcaggtggtt ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9841 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
     9901 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
     9961 ggatattaat ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc
    10021 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10081 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10141 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10201 attaagtaat ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg
    10261 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10321 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10381 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10441 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt
    10501 ggttactgtc ttcattctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10561 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10621 tttcccgttt tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg
    10681 tatgtcggca atgattgcga cggtggttgt cgttctggtg gtgacatttg cgaacgtctt
    10741 caccattatt cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg
    10801 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10861 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    10921 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    10981 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    11041 attacaaact tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt
    11101 taccgttgag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11161 cgtggaagcc tgccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11221 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga
    11281 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11341 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac actacgcgcg
    11401 tgaccgagtc agagagcgac cagttgataa actgcgcggt aataaacgcc ttgttcggca
    11461 cgattatctc tttacggtcc cagtcgctga tggtggtggc gcgggtgtta atttttgtca
    11521 cgctaccagt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
    11581 ggatgatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
    11641 caccgagcgc ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc
    11701 cgaccagccc gccaatcagc atcagcagat attttgtgat ggtggtgatg gcgtaacccg
    11761 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc
    11821 gcaccagctg cgtggtgatg ataaatacca gaatggcaat cagcaccgca ccgagggtaa
    11881 ttggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    11941 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa cgcagggcga
    12001 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
    12061 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
    12121 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12181 gcctgcgacg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12241 accagatggc aaccgaggtt tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac
    12301 ccaccgccga cgccagaatg gcaaccagtg gtgcgccaat catcatgttc cacagcatat
    12361 ggttggtaat gttgtcgccg ctgccttctt tgttgagata caacggaata ccggcttttt
    12421 tcaggcttag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12481 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc
    12541 cgatgctcat caggtagtag cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    12601 gagcgataaa caagccgttc gggcgggcaa aggtggcgca aatcatcact acccacagca
    12661 gcggcacggt ggccgttaca ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct
    12721 cgcgcaagcc gtaacccagc gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg
    12781 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    12841 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    12901 gaatcaacgc gccaaacagc ggcaaaattg tctctttgct ggtcagcatc atcacactgg
    12961 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13021 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg
    13081 ttgcttcgtt cacctctttc agcgcatcct ccagttgccc gttggagact ttcagcttgg
    13141 tcagttccaa tagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13201 tgcgcagttg tgcttccaga atacggtttt gctcggcggt cagcggctga ccgtcggcct
    13261 gatggatttg ccgtagcagc ggctgtttat taagtaaatc ctcataacgt aaccgttgca
    13321 cacgcaactg cgccatttcg gtatcaagct gttgtggttt tggcatttct ggtagccgtg
    13381 ccacctgcgc ccgcagcgct tcgccgagca ggttggacga tcccagccat tgcgactgtt
    13441 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    13501 gcgaggcaac gagctccatc cgctgcgcct gttgattcag cgctgccgat agttcgcggt
    13561 taattttgaa ttgcgcgacg atatctttcg gcaaatcggc actgttttct gccagtaatt
    13621 cggtactttc cagcgcccgc tccgcctcaa gctgacgttg gctattcaac tgattacgta
    13681 agccctgcaa atacgcatcc agttgctggc tctctttttc cgccagctct gagcgtaagc
    13741 gcgctaattc ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg
    13801 ccttaagacg tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat
    13861 tgccggtaag cgttcccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    13921 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    13981 gggcctgacg gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg
    14041 cgtcggtaga cattcccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    14101 cgcgcagagt agcggagagt ttcggataat tatcgataac ttgctgatat tgtttgatgc
    14161 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14221 cttccggctg tgcgggtttc gccgcttttg cctgctccag ttcctgagtg atttgtttgc
    14281 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    14341 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    14401 ccaatggcct ggcgtcgtgt tctgcttcga tctcttcagc aggaagtggg gcgggttcag
    14461 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    14521 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    14581 tggaaccgag tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    14641 tttccccggc aggccaggtc cagcgcttga tgataccttc gcgcggcggc gtaatggtgc
    14701 ccgcccagac cgtctcaatg ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg
    14761 ggccaaattc ggtatcgaaa aggcaaatca cgcgttcgtt acgggcaaac agattcggca
    14821 cgttctgagc ggtgagatgg ttaacggaaa agagatcgcc cggcacgtag atcatctcac
    14881 gcagaatacc gttgcacggc atgtgtacgc gatggtagtc acgcggggag aggtaagtgg
    14941 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    15001 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15061 ggctgataac gccatcggca ggcatgacca gtacattcgg atcggtatcg attgggcgta
    15121 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15181 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15241 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    15301 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    15361 tgttttgtcg ttcctgatcc ggccaacatg ccggatcctg aaaaaaaggg gacgattcta
    15421 acgacggtta gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct
    15481 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    15541 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    15601 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    15661 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    15721 agtgatacag ccgagcggcg gtcgtggtgt gctgaccgag ccccgagttg tcagagacat
    15781 cgttggtcag gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttaccga
    15841 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttca
    15901 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatgttg cggtagatat
    15961 ccatctgctc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16021 cgataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16081 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggctatgg
    16141 gtttcacgcc gtcgtagaaa tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt
    16201 ccacgatccc tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16261 ggtcgccggt taccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    16321 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    16381 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    16441 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtactg
    16501 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    16561 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    16621 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    16681 tgaccgatgc caatctgaat attctggcag aagggccgac cattgcagta catcagtccg
    16741 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    16801 tagagcgcgt gaaagcgagc acgatgggcg atcgggaagc tgaactggca acgctcgaat
    16861 tcttaaaaca gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggacagg
    16921 accgtcgttt cctgtttaaa tacatgccgg agctggaagt ctacttccac taccgttatc
    16981 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt
    17041 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    17101 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17161 atccacttgc ggcggcgtga ttttgacgct aaatcgaacg tcttgctgat aatttcagca
    17221 cttgagataa aaacgcaaaa aaaacttttt tggggggttg cagagggaaa gatttctcgt
    17281 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    17341 acgcgggaat agctcagttg gtagagcacg accttgccaa ggtcggggtc gcgagttcga
    17401 gtctcgtttc ccgctccaaa atttgaaaag tgctgcaaag cacagaccac ccaatcgcag
    17461 tatttcgaat tgcgacaagg cggcaactga acgaatcctt gggaacttac aaaagtatgt
    17521 gactaaggtg agtgaaggca gccaaggcag tagcggttcg aaagacgaag at
//
LOCUS       DADWTH010000077        17460 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.077, whole genome shotgun sequence.
ACCESSION   DADWTH010000077 DADWTH010000000
VERSION     DADWTH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.077"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            564..1334
                     /locus_tag="JNY02_002789"
     CDS             564..1334
                     /locus_tag="JNY02_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBB9434266.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JNY02_002790"
     CDS             1325..1798
                     /locus_tag="JNY02_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBB9434267.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGTLSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JNY02_002791"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JNY02_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBB9434268.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JNY02_002792"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JNY02_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBB9434269.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JNY02_002793"
     CDS             3233..3727
                     /locus_tag="JNY02_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBB9434270.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JNY02_002794"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JNY02_002794"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBB9434271.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="JNY02_002795"
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="JNY02_002795"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBB9434272.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8484
                     /locus_tag="JNY02_002796"
                     /pseudo
     CDS             7571..8484
                     /locus_tag="JNY02_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8699..10060
                     /locus_tag="JNY02_002797"
     CDS             8699..10060
                     /locus_tag="JNY02_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9434273.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNY02_002798"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNY02_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBB9434274.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNY02_002799"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNY02_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBB9434275.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="JNY02_002800"
     CDS             12610..13899
                     /locus_tag="JNY02_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBB9434276.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="JNY02_002801"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="JNY02_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBB9434277.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="JNY02_002802"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="JNY02_002802"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBB9434278.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="JNY02_002803"
     CDS             16225..16605
                     /locus_tag="JNY02_002803"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBB9434279.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17460
                     /gene="hpaH"
                     /locus_tag="JNY02_002804"
     CDS             16673..>17460
                     /gene="hpaH"
                     /locus_tag="JNY02_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBB9434280.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4294 a         4626 c         4378 g         4162 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgttt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtacgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacgattt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg cttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccacaagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgaact gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
//
LOCUS       DADWTH010000078        17409 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.079, whole genome shotgun sequence.
ACCESSION   DADWTH010000078 DADWTH010000000
VERSION     DADWTH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.079"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..537)
                     /locus_tag="JNY02_002805"
     CDS             complement(<1..537)
                     /locus_tag="JNY02_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9434281.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASM"
     gene            545..1045
                     /gene="tnpA"
                     /locus_tag="JNY02_002806"
     CDS             545..1045
                     /gene="tnpA"
                     /locus_tag="JNY02_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBB9434282.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(1084..1431)
                     /gene="grxD"
                     /locus_tag="JNY02_002807"
     CDS             complement(1084..1431)
                     /gene="grxD"
                     /locus_tag="JNY02_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBB9434283.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1766..2593
                     /gene="mepH"
                     /locus_tag="JNY02_002808"
     CDS             1766..2593
                     /gene="mepH"
                     /locus_tag="JNY02_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBB9434284.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2721..3302
                     /gene="sodB"
                     /locus_tag="JNY02_002809"
     CDS             2721..3302
                     /gene="sodB"
                     /locus_tag="JNY02_002809"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBB9434285.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3448..4617)
                     /locus_tag="JNY02_002810"
                     /pseudo
     CDS             complement(3448..4617)
                     /locus_tag="JNY02_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4783..4872)
                     /gene="ynhF"
                     /locus_tag="JNY02_002811"
     CDS             complement(4783..4872)
                     /gene="ynhF"
                     /locus_tag="JNY02_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBB9434286.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5171..6196
                     /gene="purR"
                     /locus_tag="JNY02_002812"
     CDS             5171..6196
                     /gene="purR"
                     /locus_tag="JNY02_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBB9434287.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6193..7125)
                     /locus_tag="JNY02_002813"
     CDS             complement(6193..7125)
                     /locus_tag="JNY02_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9434288.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7238..8449
                     /locus_tag="JNY02_002814"
     CDS             7238..8449
                     /locus_tag="JNY02_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBB9434289.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8740..9888
                     /gene="cfa"
                     /locus_tag="JNY02_002815"
     CDS             8740..9888
                     /gene="cfa"
                     /locus_tag="JNY02_002815"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBB9434290.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9928..10569)
                     /gene="ribE"
                     /locus_tag="JNY02_002816"
     CDS             complement(9928..10569)
                     /gene="ribE"
                     /locus_tag="JNY02_002816"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBB9434291.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10784..12157
                     /gene="mdtK"
                     /locus_tag="JNY02_002817"
     CDS             10784..12157
                     /gene="mdtK"
                     /locus_tag="JNY02_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBB9434292.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12198..13454)
                     /locus_tag="JNY02_002818"
     CDS             complement(12198..13454)
                     /locus_tag="JNY02_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBB9434293.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYCLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13762..13838
                     /locus_tag="JNY02_002819"
     tRNA            13762..13838
                     /locus_tag="JNY02_002819"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13796..13798,aa:Val,seq:gac)
     gene            13843..13919
                     /locus_tag="JNY02_002820"
     tRNA            13843..13919
                     /locus_tag="JNY02_002820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13877..13879,aa:Val,seq:gac)
     gene            14027..14332
                     /locus_tag="JNY02_002821"
     CDS             14027..14332
                     /locus_tag="JNY02_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBB9434294.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14458..16062
                     /locus_tag="JNY02_002822"
     CDS             14458..16062
                     /locus_tag="JNY02_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBB9434295.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(16074..16886)
                     /locus_tag="JNY02_002823"
     CDS             complement(16074..16886)
                     /locus_tag="JNY02_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434296.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16890..17409)
                     /locus_tag="JNY02_002824"
                     /pseudo
     CDS             complement(16890..17409)
                     /locus_tag="JNY02_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
BASE COUNT         4310 a         4490 c         4135 g         4474 t
ORIGIN      
        1 cattgatgct gaagggtgaa ccggagttga tacttcactt tctgtctgaa tactgatgta
       61 ccacgtaccg caggactggc tgacagtgac atttttcaca actcccgtga cctgacggct
      121 gttccggtag cgcatccagc caagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc ggaatgcagc attctgtccc cgctttttga atcggggaaa
      241 agcagcccgc ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggcgaat ctttaagcca ttgcgtttca gtggcgtttt tccactcaac
      361 cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
      421 ctgaagcgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
      481 acgctcctgt tgaccaccgg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg
      541 tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt
      601 tttcctgatg catgttcacc tggtctttgt caccagatac cgacgccaga tttttgacca
      661 tgacacgaca gaaaaactac gcacttactt ttcaaatgta tgtgctgatt ttgcagctga
      721 actggttgaa atggatggcg aaccagatca cgtccatttg ttaattaact atcctcccaa
      781 actggcgata tccagtctgg taaacagcct caaaggcata tcgaataggt tactgcgacg
      841 agatcgacca gatattgcag tcaggtatta ctacaaaggc gttttgtgga gtcctggcta
      901 ttttgccagt agctgcgaag gtgcgccaat atccgtcatc cgccaataca ttgaacaaca
      961 gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag gacgaggttt
     1021 tacggcgcac cggataaagg actgacggat gcccgttcgc atccgtcagt attgcaggac
     1081 ggattattcc gcgtccggct cttcagactt gtatttagcg gcagtttctt tgatcagctg
     1141 ctgcagttcg ccacgctgat acatttcgat cacgatatca caaccgccga ccagctcgcc
     1201 gtcaacccac agttgcggga aggtcggcca gttagcatat ttcggcagtt ccgcacgaat
     1261 gtccggattc tgcagaatat caacataggc aaaacgttcg ccacatgcgg caagcgcctg
     1321 gactgcctgg gcagagaaac cgcagctcgg cagtttcggt gaacctttca tgtacagcag
     1381 gatcgggttt tcagcaatct ggcgttggat tttttcgata gtggtgctca ttgtcttgct
     1441 tcctcaaact tctttacggc agtaatctga cattgtagcg ggtcagtgcg gcatcggaaa
     1501 ataacatttt catcacgctt ttgctatttt atccctttgc tctatttgtt gcatttcaaa
     1561 tattcgtttc ttctctgatg cgcggcaaac tggcgtattt ataaccattt ttagctttca
     1621 ctgctgcatt tttttgcgct cgccaacgaa acgtattttt taacaataaa agctattaac
     1681 tttctcttct tctatgcatt agaatcatca agttttgtaa atcagacgca ggcatgatag
     1741 acctgcctta acagagggac gctcagtggc gcggataaac cgtatttcga tcacgctctg
     1801 tgctttactt tttaccaccc tgcctttaac gcctatggcc catgcttcaa agcaagccag
     1861 ggagagttct gctaccgctc atatcaccaa aaaagcagat aaaaagaaaa gcacggcaac
     1921 caccaaaaag acccagaaag ccagccagaa aacagcgaaa aaagccgcca gtaaaagtac
     1981 gaccaaaagc aaaaccgctt cttccgttaa aaaatcttcc attaccgctt ctaaaaacgc
     2041 caaaactcgc agcaaacacg ccgtcaataa aacggcctca gccagcttta ccgaaaagtg
     2101 taccaaacgt aagggctata aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac
     2161 ccttgccgac gcgcacaaag cgaaggtgca aaaagccacg aaagtggcaa tgaataaact
     2221 gatgcagcaa attggtaagc catatcgttg gggcggcagc tcaccgcgta ccggttttga
     2281 ttgcagcggc ctggtttatt acgcttataa agatttggtg aaaattcgta ttccgcgtac
     2341 ggcgaatgaa atgtatcacc tgcgtgatgc aggacctatc gaacgtagtg agctgaaaaa
     2401 cggcgacctg gtctttttcc gtactcaggg acgcggtaca gccgatcatg tcggcgtgta
     2461 tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt caggaaattc aaatcacttc
     2521 tctcagtgaa gactactggc agcgccacta tgttggcgct cgtcgggtaa tgaccccaaa
     2581 aacacttcgc taaaacttta ccctgttgtt acggcaacag ggtaagttca tcttttgtct
     2641 caccttttaa tttgctaccc tatccatacg cacaataagg ctattgtacg tatgcaaatt
     2701 aataataaag gagagtagca atgtcattcg aattacctgc actaccatat gctaaagatg
     2761 ctctggcacc gcacatttct gcggaaacta tcgagtatca ctacggcaag catcatcaga
     2821 cttatgtcac taacctgaac aacctgatta aaggtaccgc gtttgaaggt aaatcactgg
     2881 aagagattat tcgcagctct gaaggtggcg tattcaacaa cgcagctcag gtctggaacc
     2941 atactttcta ctggaactgc ctggcaccga acgccggtgg cgaaccgact ggaaaagtcg
     3001 ctgaagctat cgccgcatct tttggcagct ttgccgattt caaagcgcag tttactgatg
     3061 cagcgatcaa aaactttggt tctggctgga cctggctggt gaaaaacagc gatggcaaac
     3121 tggctatcgt ttcaacctct aacgcgggta ctccgctgac caccgatgcg actccgctgc
     3181 tgaccgttga tgtctgggaa cacgcttatt acatcgacta tcgcaatgca cgtcctggct
     3241 atctggagca cttctgggcg ctggtgaact gggaattcgt agcgaaaaat ctcgctgcat
     3301 aataactgat ggcaaatgca gcattgcctg atgcgcttcg cttaccatgc caacgcggat
     3361 gaggcgttaa cagcgcattc ggcaagataa atcgcacgtt gtcagcaact gtaacgcaga
     3421 aggttatcct tctgcgtttt tgtttaatta gctgttagca acgcaaactg tttcaggttg
     3481 ttttctggct gacataaaca ccagcaataa tgccagtccc gcgacaatcg cccccgtcac
     3541 cggcacaaag ctgtatccca gcccagcgga aattaccgca ccaccagcag ctgctcccag
     3601 cgcatttcca agattaaagg caccaatatt gactgatgaa gacagacctg gcgcttcact
     3661 ggcgacatgc atcacgcgca tctgtaacgg aggtacaacc gcaaaggttg ctgcgcccca
     3721 caccaccatg ctaatagctg cgccgaactc attgcgggcc aggaacggga ttgccagcat
     3781 aatcaccatc agcagcaaca aaaagccttt caacgtgccg ttaactgaac gatctgccag
     3841 tttgccgccg agatagttac cgatagagaa tccgacacca atcagcacca gcattgccgt
     3901 gacgaacacc ggtgttgcgt gggtaatact ttgcagtacc ggagagatat aggtgtagag
     3961 ggtaaacatt gcaccagctc ccagtaccgt cgtcagcaat gcagacagca cctgcggacg
     4021 cattaatacc gccagctctt ttttcacttc aggtcgtgcc cctgtaccac ctttaggtaa
     4081 tgagaagaac agacttacca ttgaaatcac tcccagcccc gccgttgcca gaaatgacat
     4141 ccgccagccg atggtttcac ccaacctggt cgccgccggc acgccaccga tatttgccag
     4201 ggttaacccc ataaacatag tggcaactgc gctggcctgt ttatgttttg gcaccacgct
     4261 tgcggccacg actgaaccca aaccaaaaaa tgctcagtga ttcaggctgg tcaaaatacg
     4321 tgaaagcatc agggtcatat aatccggcgc gatggcggaa agaacgttgc ctagcgtgaa
     4381 aattgccatc aggaaaatca acgcactgcg gcgggcacga tgagaaagta gaagcgtcat
     4441 cagcggcgcg cccaccatta cgccaactgc ataggcactg attaacattc cggcagcggg
     4501 aatcgagaca tccacaccgc gcgcaatgac gggcaacaag cccattggcg agaactccgt
     4561 tgtcccgata ccaaacgcgc caatcgccag cgccagcaac ggatagttaa ttttcatgcc
     4621 ttatctccac ctcttcgcgt cattacgcga tattcattaa agtggcgaaa gcatgacagc
     4681 aatcacaaaa aaatgaaaat aacaaaaaga gaaaacactt ttgccatttt gctaacaaac
     4741 aggaaggaga tgcgagggag aacgcgctcc ctcgagagga aatcagtgca gcgcggcagt
     4801 caaacccacg gctacgatca aaccgaggac gataatcgtt gttaccagtg aaaatttaag
     4861 gtcggtgctc atcaagtttt ctcctttttt attaccacac aaaaaatgat attacgcatt
     4921 tttacacact gtgatgaaaa aatctcccgt catttataat gataagtgtt tttaccactt
     4981 ccccttttcg tcaagatcgg ccaaaattcc acgcttacac tatttgcgta ctggccattg
     5041 accccttcct gacgctccgt gtcgtttttc cggcgtaccg caacactttt gttgtgcgta
     5101 aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg agctgaagtt agggtctgga
     5161 gtgaaatgga atggcaacaa taaaagatgt agcgaaacga gcaaacgttt ccactacaac
     5221 tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa gaaacgcgca acgccgtgtg
     5281 ggcagcgatt aaagaattac actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa
     5341 ccacaccaag tctatcggtt tgctggcgac cagcagcgaa gcggcctatt ttgccgagat
     5401 cattgaagca gttgaaaaaa attgcttcca gaaaggttac accctgattc tgggtaatgc
     5461 gtggaacaat cttgagaaac agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga
     5521 tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg ctggcgatgc tggaagagta
     5581 tcgccatatc ccaatggtgg tgatggactg gggtgaagca aaagctgact tcaccgatgc
     5641 ggtcattgat aacgcgttcg aaggcggcta catggccggg cgttatctga ttgaacgcgg
     5701 tcaccgcgaa atcggcgtca tccccggccc gctggaacgt aacaccggcg caggccgcct
     5761 tgccggtttt atgaaggcga tggaagaagc gatgatcaag gtgccggaaa gctggattgt
     5821 gcagggtgac tttgaacctg aatccggtta tcgcgccatg cagcaaatcc tgtcgcagcc
     5881 gcatcgccct actgccgtct tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc
     5941 tgctgatgaa atgggcctgc gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt
     6001 gcgcaacgcg cgctatttta cgccggcgct gaccacgatc catcagccaa aagattcgct
     6061 gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca
     6121 gtccattgaa gtgcatccgc gtttgattga acgccgctcc gtggctgacg gcccgttccg
     6181 cgactatcgt cgttaatcac ccgttgcggg agtctcttcc ggctcccgca gccactcctt
     6241 attcagcgtc tcactatcgc cgagatactc aagcagccag gttaacgcag gcgacatatc
     6301 attttgctgc catgtcagac aacatgccga atccgggaag gggttttcca gttcaagtgc
     6361 tacccacttc ccctcattaa gccacggttt ggcgaaatgt gttggcacca tccctatgca
     6421 taatcctgcc gagatacagg ttgccgatga ttcccagtca ggtacgacaa gtcttttttg
     6481 gttatccagc aaccaggtaa tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa
     6541 cgacggccag ttgcgcaacg tatcatcgct gaacgggcca tccatcaacg ccagcgggtg
     6601 gtgactggca acaacgcaac tccagcttag catccccata tcccggaagg cataacgacc
     6661 gcctaccgga atcgcgcgtg ttgcgccaat tgccagttcc acgcgcccgt cggaaagcgc
     6721 atcccagaca ccgttgaaca cttcctgaaa gacaagaagt tcaacatcat caaaatggcg
     6781 ataaaaatca acgatcatct gccgtgtacg ttctggcctg acaatattat ccactgcgat
     6841 agctaactga ccgcgccagc cgttcgctat ctgctgacat tgctggcggg tgatctgcat
     6901 ttttttgaca acagagcgcc cttctttgag aaaccacgct ccagcagcgg tcagctccac
     6961 atcacggtgc cgtcgttcaa agagcggtac cgccagccac tcttccagct gacgcacggt
     7021 atagctgacc gcagaaggaa cgcgatgcag ctcctgtgcc gcagcgctaa aactaccatt
     7081 acgcgctacc gcatcaacaa cttcgagtga atattctgac cacatagtca gcctgcaaaa
     7141 tttttgaaac cagtcatcaa atattaccgt ttcacaacac taatttcact ccctacactt
     7201 tgcggcggtg tttaattgag agatttagag aatatacatg caacctggga aaagattttt
     7261 agtctggctg gcgggtttga gcgtactcgg ttttctggca accgatatgt atctgcctgc
     7321 tttcgccgcc atacaggccg acctgcaaac gcctgcgtct gctgtcagtg ccagccttag
     7381 tctgttcctt gccggttttg ccgcagccca gcttctgtgg gggccactct ccgaccgtta
     7441 tggtcgtaaa ccggtgttat taatcggcct gacaattttt gcgttaggta gtctggggat
     7501 gctgtgggta gaaaacgccg ccacgctgct ggtattgcgt tttgtacagg ctgtgggggt
     7561 ctgcgccgcg gcggttatct ggcaagcatt agtgacagat tattatcctt cacagaaagt
     7621 caaccgtatt tttgcgacca tcatgccgct ggtgggtctg tctccggcac tggctcccct
     7681 gttaggaagc tggctgctgg tccatttttc ctggcaggcg attttcgcca ccctgtttgc
     7741 cattaccgtg gtgctgattc tgcctatttt ctggctcaaa cccacgacga aggcccgtaa
     7801 caatagtcag gatggtctga cctttaccga cctgctacgt tctaaaacct atcgcggcaa
     7861 cgtgctgata tacgcagcct gttcagccag tttttttgca tggctgaccg gttcaccgtt
     7921 catccttagt gaaatgggct acagcccggc agttattggt ttaagttatg tcccgcaaac
     7981 tatcgcgttt ctgattggtg gttatggctg tcgcaccgca ctgcagaaat ggcaaggcaa
     8041 gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc gtcattgcga cctgggctgc
     8101 gggcttcatt agccatgtgt cgctggtcga aatcctgatc ccattctgtg tgatggcgat
     8161 tgccaatggc gcgatctacc ctattgttgt cgcccaggcg ctgcgtccct tcccacatgc
     8221 aaccggtcgc gccgcagcgt tgcagaacac tctgcaactg ggtctgtgct tcctcgcaag
     8281 tctggtagtt tcctggctta ttagtatcag cacgccattg ctcaccacca ccagcgtgat
     8341 gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg caacgttgtg aagaagttga
     8401 ctgcccgaat catggcaatg ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat
     8461 atacttatac ttaggctgct aacaaaattt tgttgtatga ttgaaattag cggcctatac
     8521 taatttcgag ttgttaaagc tacgataaat attatgtttt tacggggaca ggatcgttcc
     8581 cgactcacta tggatggtca tttcggcaag ggttcctcct ttccctctgt tctacgtcgg
     8641 attatagact cgcggttttt tctgcgagat ttctcacaaa gcccaaaaag cgtctacgct
     8701 gttttaaggt tctgatcacc gaccagtgat ggagaaacta tgagttcatc gtgtatagaa
     8761 gaagtcagtg taccggatga caactggtac cgtatcgcca acgaattact tagccgtgcc
     8821 ggtatagcca ttaacggttc tgccccggcg gatattcgtg tgaaaaaccc cgattttttt
     8881 aaacgcgttc tgcaggaagg ctctttgggg ttaggcgaaa gttatatgga tggctggtgg
     8941 gaatgtgacc gactggatat gttttttagc aaagtcttac gcgcaggtct cgaaaaccaa
     9001 ctcccccatc atttcaaaga cacgctgcgt attgccggcg ctcgtctctt caatctgcag
     9061 agtaaaaaac gtgcctggat agtcggcaag gagcattacg atttgggtaa tgacttgttc
     9121 agccgcatgc ttgatccctt catgcaatat tcctgcgctt actggaaaga tgccgataat
     9181 ctggaatctg cccagcaggc gaagctcaaa atgatttgtg aaaaattgca gttaaaacca
     9241 gggatgcgcg tactggatat tggctgcggc tggggcggtc tggcgcacta catggcatct
     9301 aactatgacg taagcgtggt cggcgtcacc atttctgccg aacagcaaaa aatggctcag
     9361 gaacgctgta aaggcctgga tgtcaccatt ttgctgcaag attatcgtga cctgaacgac
     9421 cagtttgatc gtattgtttc tgtggggatg ttcgagcacg tcggaccgaa aaattacgat
     9481 acctattttg cggtggtgga tcgtaatttg aaaccggaag gtatattcct gctccatact
     9541 atcggttcga aaaaaaccga tctgaatgtt gatccctgga ttaataaata tatttttccg
     9601 aacggttgcc tgccctctgt acgccagatt gctcaatcca gcgaacccca ctttgtgatg
     9661 gaagactggc ataacttcgg tgctgattac gatactacgt tgatggcgtg gtatgaacga
     9721 ttcctcgccg catggccaga aattgcggat aactacagtg aacgctttaa acggatgttt
     9781 acctattatc tgaatgcctg cgcaggtgct ttccgcgccc gtgatattca gctctggcag
     9841 gtggtgttct cacgcggtgt tgaaaacggc cttcgagtcg ctcgctaaag gctattctat
     9901 cgccccctct tcgggggcga gttcagatca ggcttctgta cctggttgat tcatggcatt
     9961 ttctcgtgcc gccagcacac gttctaccgt atctaccact gcctgagttt gtggatcgat
    10021 ttcaatgttg acgcgtgcgc caagtttttt cttcccaaga gtcgtgcgtt ccagtgtttc
    10081 cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc
    10141 gtcgatgcca ataaatcctt tgtacaggat atatttcatc aactgactat cctggacttt
    10201 aaaccagatc tggcgattat tttccgaggt taatattttc gccacttcag cggtggtcat
    10261 aatatgacct gacattaagt gtccgccaat ttcatcactg aatttcgccg cacgctcaac
    10321 gtttacccaa tcccccactt ttaaatcgcc aagattggta atgcgtaacg tttctttcat
    10381 caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg
    10441 cgccacggaa gcaccggttt ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg
    10501 cgtacgaaaa tttggtttct cgtcaatcga caccagtttt gcggtgccct gtacaatccc
    10561 cgtaaacata cttacaactc ctgaaatcag ttaagacatt ctgttcagca caatagcagg
    10621 tggaaaacgc ccttaccagt gaaggggtaa gaatggctat tttttcactg gagaattaat
    10681 aaatcctcgc tacaatagac tgaatttccc ctgcttcttc tttttgctgc ccattcaggc
    10741 ggctttttag tctctcatat aactacaaat aaaaggtgtt cacgtgcaga agtatatcag
    10801 tgaagcgcgt ctgttattag cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac
    10861 tgcgatgggt tttgtcgata ccgtgatggc gggcggctat agtgccaccg acatggcggc
    10921 ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc tttggtcacg gactgctgct
    10981 ggcattaacg ccggttatcg cgcaattaaa tggttccggt cgacgtgagc gcattgcgca
    11041 tcaggtgcga caaggtttct ggctggcagg ttttgtttcc gttctcatta tgctggtgct
    11101 gtggaatgca ggttacatta tccgctccat ggaaaacatc gatccggctc tggcggacaa
    11161 agccgtgggt tatctgcgtg cgttgttgtg gggcgcgccg ggatatctgt tcttccaggt
    11221 tgcccgtaac caatgtgaag gtctggcaaa aaccaagccg ggtatggtaa tgggctttat
    11281 cggcctgctg gtgaacatcc cggtgaacta tatctttatt tatggtcatt tcggtatgcc
    11341 tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg gtgtattggg tcatgttcct
    11401 tgccatggtt tcttacatta aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg
    11461 caccgctaag cccgatcctg cggttatgaa acgactgatt caactcggtt tgccgattgc
    11521 gctggcactg ttctttgaag tgacactgtt tgccgtcgtg gctctgttag tgtctccgct
    11581 cggtattgtt gatgtcgcag gacaccagat tgccctgaac tttagttcac tgatgtttgt
    11641 gcttccaatg tcgctggcgg cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg
    11701 ctcaacgctg gatgcgcaaa ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc
    11761 aaccctgacg gccattttca cggtttcact gcgggagcaa atcgccctgt tgtacaacga
    11821 caatcccgag gttgtaacgc tggctgcgca tttgatgttg ctggcggcgg tatatcagat
    11881 ttctgactca atccaggtga ttggcagtgg gattttgcgt ggttataaag atacgcgttc
    11941 cattttctat atcaccttta cagcttactg ggtgctgggc ttgccaagcg gctatattct
    12001 ggcactgacc gatctggtcg ttgaacctat ggggccagct ggcttctgga taggctttat
    12061 tatcggcctg acatcggcag ccattatgat gatgctgcgt atgcggttcc tgcaacgtat
    12121 gccgtcagcc atcattctgc aacgagcgtc ccgctaataa agacaaggcg caaccttcac
    12181 gggttgcgcc tgtgttttta cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc
    12241 tgtaatttca gagcgttaca cagagtttgc cgactggcat ctgttatctc aacgcgtata
    12301 tccccgttaa cttccccttt cacattgatg aagtcaggtg aaatttcttt cgcactggtt
    12361 tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc
    12421 gcaccagtga tcagccacag ttttccgcca ttcagattga tgttaccaac aatgaaagtg
    12481 acgctgtcac cttttaccgt actgccgtcg agaatcaaca caccgccctg ctgtacagta
    12541 atcgttgatt gttgcagctc tgcgccataa ccaataatca ttgaagcgcc gtccagtaat
    12601 gcaacactcc cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc
    12661 gcgctggtgg gtgacaacag gatcactgtt ccaccttgta ttaccgcatt ttccagcgta
    12721 ccggcatgca cttcagcccg atcccccaca ttcaccgaca ggttttgcgt cttaccggaa
    12781 ctgtagagct gaaggccatc cgtccccaga caataaatgg atccattctc aacaatagtg
    12841 tcagagtctt caccaccgag attattcatg gtgccaccag caagcaacgt atcactggct
    12901 ttcccggcca gcataaaaac gccgccctga ttaatctcac aattgctggc ttctccactt
    12961 ggatacacca acaattcacc accttcatct accacgaccg ctgaggttgt cccattaacc
    13021 aacaggccgc cctcttgatc gagaatgatt ttttccgcgc gatgtccttc cagtacacgc
    13081 aggttaccgc cattttccag caacaaaccg taggcataac cctgatcaac gctgaattca
    13141 ccttcgggat ggcggccatt aaccgtagcg agcgtagaga gactaattgc gcctccctcg
    13201 gactgaacaa tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc attgttaccg
    13261 tactgtgtgc atttggcgat cccaccggtt gccactttca accagccagc ccccgtgacc
    13321 acggtatttt cggctcttcc gccagcttca acaatcaact cgccttcagt aagttgcgtc
    13381 gcgccaatga cctcgccggt cttaacaatt tgcacattcc cgtcgctcat caagtttttc
    13441 gcatcagatc ccatgattta ttcctttgct gcatctgtgt gcctttattg ctacctaagt
    13501 gtaaaggcta cggaggattt atccacgaca gatttgagat ggtggcaaac aactctgttt
    13561 aaactctgat acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat
    13621 cgcccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa
    13681 tagcgcagaa aattacgttt tgcctcttgc cacctttcca ctctgccgct aatattcgtc
    13741 cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat
    13801 ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg
    13861 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag
    13921 attttcttaa tctggtcttt tcctttttcc ctctgtttct tctctgtatc caatacgtta
    13981 aaagatttac actgtcttcg tctgcgttat cagaaggaga atcgctatgg caactttgtt
    14041 acaatttcat tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaacc
    14101 acttgctgag tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga
    14161 aaagaaccac gaagccggtg ggatctacct tttcactgat gaaaaaagcg cccttgccta
    14221 tctggaaaaa catactgccc gactgaaaaa tctcggcgtt gaggaagttg tcgccaaagt
    14281 tttcgatgtc aatgagccac ttagtcaaat caatcaggca aaactcgcct gacagaattt
    14341 aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc
    14401 tcaaccaaac ttatctgata acactaaatt cgaaagaatg cgtacaggta agtaacaatg
    14461 aaaaaaattg ctattgtggg tgccgggcct acggggatct acaccttatt ctcgcttcta
    14521 cagcaacaaa ctccactttc tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg
    14581 atgccataca gtgatgagga aaactcaaaa atgatgctgg caaatattgc cagtattgaa
    14641 ataccgccga ttaattgtac gtatctcgaa tggctacaaa agcaagaagc cagccatctc
    14701 cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc gcgaattctg
    14761 ctgggcgaat atttccgcga tcaattttta cgattagtag accaggcacg acagcaaaaa
    14821 tttgcagtgg ctgtttatga atcatgccag gttaccgatc tgcaaattac aaatgctggc
    14881 gtcatgctcg ctacaaatca ggatttaccc agcgagacgt ttgatttagc ggtgattgcc
    14941 acgggtcacg tctggcctga tgaagaagaa gcaaccagaa cgtattttcc cagcccgtgg
    15001 tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg
    15061 agcggactgg atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat
    15121 aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa agctaaatat tacgttgatg
    15181 tcgcgcacgg gtattttacc cgaagccgat ttctattgcc ctattccctg cgagccctta
    15241 cacatcgtca ccgatcaggc attaaatgct gagattcaaa aaggtgaaga gggccttttg
    15301 gatcgggtat ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa
    15361 cgcatagcct tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc
    15421 aaacaacgcg acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa
    15481 cgagaaaaac atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag
    15541 gaaattgttc ctcatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgccagg
    15601 gttttcatcg ataattatgc ggcaatccct tcagagtcta ttcgtcgact actggcctta
    15661 cgtgaagcgg ggatcattca tattctcgcc ctcggtgaag actacgaaat ggaaattaat
    15721 gagtcgcgca ccgtcctgaa aacggaagac aacagctact cgtttgacgt ttttattgat
    15781 gcccgcggac agcgtccgct taaagtgaaa gatattcctt tccctgggct acgcgagcaa
    15841 ttacagaaaa caggggatga aatccctgat gttggcgaag attatacgtt acagcaaccc
    15901 gaagatattc gtggacgcgt agcgttcggc gcgttgccct ggctgatgca cgaccagcct
    15961 ttcgttcagg gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta
    16021 aagcctgcat cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac
    16081 tcaacaaact gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca
    16141 ccttcaatgg cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt
    16201 ccccctagcc cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca
    16261 ccgctggcaa tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag
    16321 ctggcgattt gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa
    16381 acgcgtactc gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc
    16441 gaagaattac cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta
    16501 aagccgacac ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca
    16561 gcggttgcga ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg
    16621 gcaaactgac tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa
    16681 acttcctgat gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga
    16741 ctctccgccc agtccgtata tccttcctca atcagtgcat gacgaatatc ggcataacta
    16801 ccgccgcgtg ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct
    16861 cgcaggtctg ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt
    16921 ttaaaggttt cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa
    16981 aagaggctta taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga
    17041 aacacatctc ccacggcttg cggatagaga cacaacagcc ccgtcaatag taacaacggc
    17101 agcaatccat acatgacacc aacataggcg acctgctgta agggattaaa tttagactgt
    17161 gttgttgccg ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt
    17221 tgttttgccg ctcgttccag ccacccctga cgacgaatgc gatagtggtg accattatcc
    17281 ccaacggcat tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa tcagcaaact
    17341 tcatgcaccg caaccagact tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc
    17401 agcccactt
//
LOCUS       DADWTH010000079        17383 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.080, whole genome shotgun sequence.
ACCESSION   DADWTH010000079 DADWTH010000000
VERSION     DADWTH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.080"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(40..771)
                     /gene="rlmB"
                     /locus_tag="JNY02_002825"
     CDS             complement(40..771)
                     /gene="rlmB"
                     /locus_tag="JNY02_002825"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBB9434297.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(951..3392)
                     /gene="rnr"
                     /locus_tag="JNY02_002826"
     CDS             complement(951..3392)
                     /gene="rnr"
                     /locus_tag="JNY02_002826"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBB9434298.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3431..3856)
                     /gene="nsrR"
                     /locus_tag="JNY02_002827"
     CDS             complement(3431..3856)
                     /gene="nsrR"
                     /locus_tag="JNY02_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBB9434299.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4061..5359)
                     /gene="purA"
                     /locus_tag="JNY02_002828"
     CDS             complement(4061..5359)
                     /gene="purA"
                     /locus_tag="JNY02_002828"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBB9434300.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5463..5660)
                     /locus_tag="JNY02_002829"
     CDS             complement(5463..5660)
                     /locus_tag="JNY02_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBB9434301.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5742..6746)
                     /gene="hflC"
                     /locus_tag="JNY02_002830"
     CDS             complement(5742..6746)
                     /gene="hflC"
                     /locus_tag="JNY02_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBB9434302.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6749..8008)
                     /gene="hflK"
                     /locus_tag="JNY02_002831"
     CDS             complement(6749..8008)
                     /gene="hflK"
                     /locus_tag="JNY02_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBB9434303.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8094..9374)
                     /gene="hflX"
                     /locus_tag="JNY02_002832"
     CDS             complement(8094..9374)
                     /gene="hflX"
                     /locus_tag="JNY02_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBB9434304.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9450..9758)
                     /gene="hfq"
                     /locus_tag="JNY02_002833"
     CDS             complement(9450..9758)
                     /gene="hfq"
                     /locus_tag="JNY02_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBB9434305.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9844..10794)
                     /gene="miaA"
                     /locus_tag="JNY02_002834"
     CDS             complement(9844..10794)
                     /gene="miaA"
                     /locus_tag="JNY02_002834"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBB9434306.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQVVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10787..12634)
                     /gene="mutL"
                     /locus_tag="JNY02_002835"
     CDS             complement(10787..12634)
                     /gene="mutL"
                     /locus_tag="JNY02_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBB9434307.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12644..13981)
                     /gene="amiB"
                     /locus_tag="JNY02_002836"
     CDS             complement(12644..13981)
                     /gene="amiB"
                     /locus_tag="JNY02_002836"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBB9434308.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14000..14461)
                     /gene="tsaE"
                     /locus_tag="JNY02_002837"
     CDS             complement(14000..14461)
                     /gene="tsaE"
                     /locus_tag="JNY02_002837"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBB9434309.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14433..15980)
                     /gene="nnr"
                     /locus_tag="JNY02_002838"
     CDS             complement(14433..15980)
                     /gene="nnr"
                     /locus_tag="JNY02_002838"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBB9434310.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15979..17118
                     /gene="queG"
                     /locus_tag="JNY02_002839"
     CDS             15979..17118
                     /gene="queG"
                     /locus_tag="JNY02_002839"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBB9434311.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17101..17154)
                     /locus_tag="JNY02_002840"
     CDS             complement(17101..17154)
                     /locus_tag="JNY02_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434312.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3938 a         4955 c         4371 g         4119 t
ORIGIN      
        1 accatccaaa tctggatggc ttttcataat tcagaggaac tagctgcgct gacgaaccgc
       61 ttcaaataag caaattccgg ttgcaaccga aacgttcagg gaagaaacac ttcctgccat
      121 tgggatgctg atcaactcat cgcaatgttc acgggtcagg cgacgcatac cttcaccttc
      181 cgcgcccatc accagcgcca ggcgtccggt cattttgctc tgatagagtg tatgatccgc
      241 ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc aacatacgca tggtgcgcgc
      301 aaggttagtc acccgaatca gcggaacgct ttctgccgcg ccgcaggcta cttttttcgc
      361 cgtagcgttg agctgtgcgg agcgatcttt cggcacaatc accgcatgaa cgcctgcggc
      421 gtccgcacta cgcaggcacg cgccgaggtt gtgcggatcg gttacgccgt cgaggatcag
      481 caggaacggt tgatcgagcg aagcgatcag atccggcaga tcgttttcct gatactgacg
      541 tcctggcttc acgcgggcga taatgccctg atgcacggca ccgtcgcttt tctcgtcgag
      601 atattggcgg tttgccaact ggataaccac gccctgagac tcaagggcgt gaatcagcgg
      661 taacagacgt ttatcttcac ggccttttaa aataaagact tcctgaaaac gttcaggggc
      721 gcgctccagc agggcctgca ctgcgtggat gccgtaaatc atttcgctca ttaatgtact
      781 cgttgtttac gttttgcctg atgcactacg tttatccggc ctacatgatc tctgcaatat
      841 attgaatttg cgtgattatg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
      901 aagcgcattt tgtcagcaat ctaaccctct tcttttaaag agggtattga tcactctgcc
      961 acttttttct tcgccgcacg cttcgctttg gtcgctgcgg ctattttctg cgttttcgcc
     1021 gatggctttt tcgcttttct cgcgtctttc ttcgccgctt tcggcttcgt ttttttctca
     1081 ccgcggaagg cgctgtctgg ctcaaagttt acctttttac cgacctgacg acgcttgccg
     1141 ccttttttgc ctgcatcgcc ttttttcgct ttctcgcgcg ccgttttacc gacgttgcgc
     1201 ggtgcgcgtt cactggagat cagactaaag tcgattttgc gctcgtccat attaaccgct
     1261 tcgacgcgaa cttccacgcg atcgcccagg cgataagtct ggccgctgga ttcccccatc
     1321 aggcgttgcc ctacctggtc aaagcgatag tagtcattgt ccagcgaaga gacatggacc
     1381 agaccatcaa tgaacaagtc gtccagacgg acgaagaagc caaagccagt gacgctggaa
     1441 attacgcctt taaagacgtt acctacctgg tcgagcatga agtcacactt cagccagtca
     1501 gcgacatcgc gcgttgcttc gtcggcacga cgttccgcca tcgaacagtg ctgacccagt
     1561 tgcaacatct cttccatcga ataatggtag ccgccggttt cagtggtgtt gccctgatgc
     1621 ccctgctctt tcgccagcag atatttaatg gcgcggtgca gcgtcaggtc aggataacga
     1681 cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca gaccgaagtg accacggttt
     1741 tctggatcgt aaatcgcctg tttcatcgag cgtagcagca tggtttgcag catttctgca
     1801 tcaggacggt cggcaaccga ctccagcagt tccgcgtagt cacgcggttc cggcttgtta
     1861 ccacccggca gctccagccc cagctccgcc agcactgaac ggaaagaggt aatcgcttcg
     1921 gtgctcggct tgtcgtgaat acggaacagt gccggttctt tcgctttctc aacgaaacgc
     1981 gccgccgaga tattcgccag aatcatgcac tcttcaatta atttgtgcgc gtcgttacgc
     2041 tgggtctgtt cgatacgttc aatacggcgt tcagcgttga aaatgaactt cgcttcttcg
     2101 ctctcaaatg agatcccacc gcgttcttca cgggctttat ccagcacttt atagaggtta
     2161 tgcaactctt cgagatgctt aaccagcggg gcgtactgct cgcgcagatc ctgatcgccc
     2221 tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg
     2281 tagaatttgt agcccgtcag gcggcctttc gacgaaaccg tcatctcgca caccatacac
     2341 aggcggtcta cctgcgggtt gagcgaacac aggccgttag agagcacttc cggcagcatc
     2401 gggataacct gcgaagggaa gtacaccgac gtgccacggt tacgcgcttc tctgtccagc
     2461 ggcgttggcg gacgcacata gtagctgacg tcggcaatcg cgacccataa acgccagccg
     2521 ccgccgcgtt ttttctcgca gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca
     2581 atggtgacca gcggtaaatc gcgcaaatcg acacggcccg cttttgcttc ttccggcact
     2641 tcttctttca gccccgcaac ctgttgctca acagcctgcg gccagatata cggaatttca
     2701 tgggtacgca gagcgatatc aaccgccatg ccggtgccca tattgtcgcc cagcacttcg
     2761 acgattttac ccaccgcttt ggtgcggcga gtcggacgct gagtcagttc gactacgacc
     2821 acaaagccca tccgcgcgcc catgatctga tcgggcggga ttaagatatc gaagctcaga
     2881 cggctatcgt caggaaccac aaagccgacg cccgcttcgg taaagtagcg accaacaatc
     2941 tggctggttt ttggcaccag tacgcggaca atacgcgctt cacgacgacc tttacggtca
     3001 gcacccagcg gctgcgccag cacctgatcg ccatgaatgc aggttttcat ctgctcgctg
     3061 gagagataca aatcatcttt acgcccttca acccgcagaa agccgtagcc atcacggtgg
     3121 ccaataacgg tacctttcac caggtcgagg cgttccggca gcgcatagca ctgacgacga
     3181 gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc
     3241 tgctcttcgc cttcaatgtg cagttctacc gccagctcat cacggctggc cggtttttca
     3301 cgtttggtta aatgttcgag gataaattcc cgactaggga tgggattcgc gtatttttca
     3361 gcttcgcgtt cctggaaagg atcttgtgac atctcggttc ctccgttgtc atctctgatg
     3421 aagattttcg tcactccaac agcaataatt tataaagcgg ttgattctct tcaaccaaat
     3481 cggcaagcgt gtagttatcc agttccgtaa gaaaactttg cacggcctta gaaagcgcct
     3541 gtttcaaccg acaggcaggt gtaatgtggc aaaactcact gctgcaattc accagcgata
     3601 agggctccag ctcgcgcacc acatcaccaa tacgtatcgc actcgccggt ttacccaggc
     3661 gaatgccgcc attttttcca cgaacagcag tcacgtagcc ggcacgacta agttgattga
     3721 ttattttgac catatgatta cgggagacgc cgtagacgtc agtcacttca gaaatactgg
     3781 tcatccgccc ttctggcaat gacgccatgt agatcagcgc acgtaatccg taatcagtga
     3841 aactcgttaa ctgcacatca acctcaaaag ggaaatcggg aaaaaataca tttatattga
     3901 tgataaacca gccacaagct gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg
     3961 aggatttaac actgtgtcgg atagcgtaat ggcttaaccg acacagcaat gtatcacact
     4021 gttagcccgg caggcaaaat atctgccagg cgtaccagaa ttacgcgtcg aacgggtcgc
     4081 gcagaatcat ggtttcagta cgatccggac cggtagagat gatatcgatc ggcacaccgg
     4141 tcagctcttc aatacgcttg atgtagttca gcgccgcctg cggcaggccg ctacgatctt
     4201 tcacgccgaa agtggattca gaccagcccg gcatggtttc gtaaatcggc tctacacctt
     4261 tccagtcgtc agctgccagc ggagtggtag tcacttcgcg accatccggc atacggtaag
     4321 ccacgcagag tttaacctct ttcaggccat ccagaacgtc cagtttagtc aggcagaagc
     4381 cagacaggga gttcagctgt accgcacgac gaacggcaac ggtgtccagc cagccggtac
     4441 gacgacgacg acccgtagtt gcaccgaatt cgttaccctg cttgcagagg aactcgccag
     4501 tttcatcaaa cagttcggcc gggaaaggac ctgcacctac acgagttgag taagctttga
     4561 ggatacccag aacgtaatca acataacgcg ggcccaggcc ggaaccggtc gccacgccac
     4621 cagcagtggt gttggaagaa gttacgtacg gataagtacc gtggtcgata tccagcagcg
     4681 taccctgcgc accttcgaac atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt
     4741 cagaaacgtc aaccaccata gaagtcagga tgtcggcaac agccatcgta tcatccagaa
     4801 ctttctggta atcaaccgct tcagctttgt agtagttaac caactggaag ttgtgatatt
     4861 ccatcacttc tttcagtttt tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca
     4921 gaccgcgacg tgccacttta tcttcataag caggcccgat accacgaccg gtggtgccga
     4981 tcgctttcgc gccacgcgct ttctcacgcg cgttatccag cgcaacgtga taatcaagga
     5041 tcagcggaca tgcttcagac agcagcagac gctcacgaac ggggatgcca cggtcttcca
     5101 gttctttcat ctctttcatc agcgcggccg gagacagcac aacaccgtta ccgatgatgc
     5161 tggttacatt ctcgcggaga atacctgatg gaataagatg gagaacggtt ttttcaccgt
     5221 tgattacgag agtatggcct gcgttgtgac cgccctggta gcgtacaaca tatttagccc
     5281 gttcagtcag aagatcgacg atcttacctt taccttcgtc accccattgg gtgcccagta
     5341 cgacgacgtt gttacccatt tttcaaaatc accgtttgct taaaaatgga ttctaccatc
     5401 gctttttcag agttacagca ctttttgcac tcaaaaatga cctcgaacag tctgtttttt
     5461 gttcagccaa tcgttttcct caacatgtag tagaccacaa cgcccgcaac cacaagtcca
     5521 ccgccaaaac gacgtaaaat attatcgggc aaattggtca tcgcagagat catcttcttc
     5581 catgccttcg ggtaaagcat cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc
     5641 agccagattg tcgaattcat tcattatcct tatagaaaaa gaaaaccacc gacatccctg
     5701 aggatgcggt ggctttattg acctgtaccg cagtcgttat attaacgcgt tgcggaagtc
     5761 ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca tgaccatcac gtcctgattg
     5821 ccagagaagc tgttctcata agcacgcagg ctacggatga atgcgtagaa gtccggatct
     5881 ttactgaatg catcagcaaa cagtttggct gcttcggcat cgccttcacc acgcataatg
     5941 cggccctgac gctcagcttc tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc
     6001 agtttttccg cttcttcctg accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg
     6061 gcgcgcatac ggttgtagat cgcttcagac acttcggtcg gcaggttgat ctgcttgata
     6121 cgcacatcga caacttcaat acccagcgcc gccatactgt tcgggttgat gaccggaact
     6181 ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg caatggcgtt atctgctgcc
     6241 ggggtagtaa cttcatcttc tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg
     6301 agggtcagac gaccacggga atcggtgacg atatctttca cgtccaggcg accaatttca
     6361 gaacgcagac ggtcagagaa cttacgtttc aacagcactt ccgcttgcga aatgtcgcca
     6421 ccacccgttg ccaggtagta acggctgaaa tcgctgatgc gccatttgat gtaagagtcg
     6481 acgatcaggt ctttcttctc tttggtcaca aagcggtcgg cctggttgtc catggtctga
     6541 atacgtgcgt cgagcatttt caccgtttca atgaacggta tcttgaaatg cagaccaggc
     6601 tcataaacca aaggtttgtt gtcatcgtca cgcagtacct taccaaaacg cagcgtaata
     6661 ccgcgctcac cttctttgac gacaaagaca gacatgtaaa gcactaccag cacgatgatg
     6721 ataatcgcga taactgactt acgcatcgtt attccccctg acgctggtag tcgttacgct
     6781 gcgcgttggc gcggcgttgg tccataatat cgccctgact ggtggacgac gtgttgcttg
     6841 ctccactggt tgtggaggaa gaggctggcg gcagacgcag cagattgctg gcaccgttat
     6901 cgctcttcgc cgcaggggcg ttaccacctt tcaacatctg gtctaacggc agaaccatca
     6961 ggttgccacc tttatcgtta accagcactt tgcgggtgtt acccaacact ttttccatcg
     7021 tctcgatata cagacgctcg cgagtaattt ccggcgcggc tttatattcc ggcagaagtt
     7081 tagcaaagcg cgccacttca ccctgagctt ccaggatggt ctgggccttg tacgcacgcg
     7141 cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg
     7201 cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg
     7261 ccgcttttac ttcttccggc ggacgagcag cctggaagtt gacgtccagc agcgtgatac
     7321 ccatgtcata cggacgaatc gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg
     7381 tacgaccttc cgtcagaatg cggtccatgg tgtatttacc gataactcca cgcagggcgc
     7441 tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac gctatacaga tatttttccg
     7501 gattggtgac gcggtactgc acgttcatct caacgcgcac tacgttctcg tccgacgtca
     7561 gcatcacacc agaagcggcc agttcacgca cggcttccac gttcaccggt ttgacttcgt
     7621 cgataaacgt cggtttccag ttcagacccg gctcaaccag atggctgaat ttaccaaagc
     7681 gtgttaccac gccgcgttcg gcttctttaa tggtatagaa accactggcc gcccagataa
     7741 tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc aagctgcggg cgcgggcctt
     7801 gcgatgaact gccaccgcca gatccggtgc ctttaccgcc gcccagacca ccgagctttt
     7861 tgctcagttt gcggaagata tcatctaaat caggtggccc ttgatcgcga ccgcctttgt
     7921 ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc
     7981 cgttattacc gggctgattc cacgccatgt ttgtgctcca tatttgttat gcggtgatcc
     8041 ctgttgtctt tcgacgccag aggatatgac tccacgcttc agacgctacg ccgttagatc
     8101 aggtaatcga tcaacgccgg ttcttgttta cagaggcgac gccagtcaac gatcggcata
     8161 cgaacttgca gacttacgct gccgtcctcc tccatccact ctttttctat tgcctgaagc
     8221 tgataaaaac gactcctcag acgcccttcc tgcggtggca gacgcaatgt atgctgcgcc
     8281 acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc
     8341 tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt cccgatcaat acgcggttcg
     8401 aaatcttcca gcatatcgat cttgttcatc accagcaggg ttgggatctc gtgagcgtcg
     8461 atctcttcaa gaaccgtatt caccgcttcg atgttttctt gtacacgcac atccgccgca
     8521 tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgct
     8581 gccaccagat cgtgcggcag gtggcgaata aaccctacgg tatctgcaag tacggtttca
     8641 ccgacatctg caacgtcaat acgccgcaac gtcgggtcga gggtggcaaa caactggtct
     8701 gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta
     8761 tatcccacca gcgaaacagt aggaacgtcg gctttgatac gcgattgccg cccctgctca
     8821 cgctgctttt caactctttc caggcgcgac tgtatctgca cgatgcgatt acgcaacaaa
     8881 cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca aacctatccc gcctttctgt
     8941 ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc
     9001 tcaacctgca acttaccctc atgggtacgc gcacgttggg cgaaaatatc taaaataagg
     9061 ccggtgcggt cgataacacg acactcgcac aaacgctcca ggttacgctc ttgcgccggg
     9121 ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg ctttgacagc ttccgcaatt
     9181 tcaactgctt taccttcacc tacaaaatac tttgggtgcg gcgctttacg gctaccggta
     9241 atcacctgca atgcttcgac accggcggaa gagaccagag attcaaactc ctggaggtct
     9301 tccatatctt tgtcttgcgt aaaatagatg tgtaccagta ccgcctgctc accagcatca
     9361 taacggtcaa acaagcgtat aaccctctaa atagatcagc ggggaacgca ggatcgctgg
     9421 ctccccgtgt aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg
     9481 gaagtattct gtgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg
     9541 ttactgtgat gagaaaccgg gcgagatggg acaacagtag aaatcgcgtg cttgtaaacc
     9601 atctggctga ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct
     9661 tgcagcttaa taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg
     9721 ttcaggaacg gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt
     9781 atttgtactt tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca
     9841 cattcagcct gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt
     9901 ttcactgtca agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc
     9961 caactgtctc gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgccttc
    10021 aaggtaagac cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa
    10081 atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg
    10141 aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg
    10201 atgcacctga tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc
    10261 cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct
    10321 tgctgccgca accggatcta ccacctgaag ttgacgatgc aatgactccc aaccttgctc
    10381 tgccgcctgt tgctcaattc tggcccgcac ttccgggtct gccgacggta gcggcgacaa
    10441 cccttccagc aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc
    10501 cgccgcggtg atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta
    10561 agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc
    10621 gttcggcttc gccgtcccga tatccatccc tttgtaaata agggcagaat caacgcttat
    10681 caactctact ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt
    10741 cggccccatc aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca
    10801 gggcttttat cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca
    10861 caagttgcgg acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc
    10921 actgcgcatg ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga
    10981 atacggactg cttcgccagg tagcctatca gttcaggaat caagatttgt aaattttgtt
    11041 ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa
    11101 tacccaattc cgctagggca gactgcgctt tttctaatgc cgatttttct tcgccagaaa
    11161 cttttagccg caacggaatc agcagcggct gggcgcaaac gggcgcttca cccggcgtca
    11221 attgtgcctg acgcagccaa cgttctgcca ctggcaagga taaaagtgaa atgttgccat
    11281 cgcgctccag caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac
    11341 tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg
    11401 gcgtttgcaa aagctggcga tacacttcac cttgctgttt ctggtagcct ggctgcgcat
    11461 tcggccaggg ggcagccgga cgactgcctg aggcgggcgc aggagagtag cgcggagcta
    11521 ccggctcacg agctgccggt tctgcaaagt gattgcgccc cgccgccacg cggttttccg
    11581 ggatggcacg cggtgcgggt tgtggttcat cgtccagcgg tagcggcgtt tccagttgct
    11641 gttgtagcac gctcagcacg ccctgataga taaaatcatg caccagacgc gactgatgga
    11701 aacgcacttc gtgtttggcg gggtgtacgt tgacgtccac ctgatgcggg tcgatctcca
    11761 gatacaacac aaatgccggt tgctgatcgg cccccagttt gtcttcgcag gcctggcgga
    11821 tcgcatgatt gatcaggcga tcgcgcatca tgcgaccatt cacgtagcaa tactgaattt
    11881 ccgccagtgc gggcgtagtg tgatttggat cggccaccca gccgcgcagg gtgagatcgc
    11941 cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc ggtgccgcaa atcgcgccta
    12001 agcgccgttc tttttgcccg ccttccggca ctgcgcggta ctgacgcaca attttaccgt
    12061 tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc aatgcggcgg atgatttcat
    12121 caatatggct aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt
    12181 agaacagatc cagcacctcc agcgtcgttc ccaccggatg cgccgccggt ttaaccgtca
    12241 cgtccatgtc gcgtccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg
    12301 aagtgagcgt caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca
    12361 ggctgataat ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgagcca
    12421 gcgccagcgc cagctcatct tttttgatac cgcagccgtt atcacgaatg cggataagtt
    12481 tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt
    12541 ccactagttc tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt
    12601 tcgccagttg tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc
    12661 gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc accctgcggc gcagattgca
    12721 tcggatgcgc aaggaagtaa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt
    12781 ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga
    12841 ccagtactga tgggatatcc ggcgagcgca gaacgccaag gctggcgtgt tctggtcgac
    12901 gtttatgtat ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc
    12961 ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag
    13021 actgactgtt cgccagcaca tcacccgccc cacccagcag ctccgactgt ttctcgtgct
    13081 gctccagcca gctggccatt tcactgttgg cgcgacggtt agagagcacc aatacggaag
    13141 cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt
    13201 tttgcttatg tgccacatca ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg
    13261 ttaaaacgcc tttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg
    13321 cgatggtgac atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc
    13381 cgtgtccggc atcaatagcg atgataattt tatcgccagt gttagccgtc gcgcgcgctg
    13441 ccggacgcgt taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa
    13501 acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt
    13561 tcgcaaccac gggcggcgga ggaggcggtg gcggcacatc ggcgttaatc gtaaagacga
    13621 cagtgtaatt gctgccattc tgccgcttca ccgcttcggt tttaccgttt tcggtaagat
    13681 cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc agagcgaatc gccttcacca
    13741 gattattgcc gctgaacaac aacggcagtc cctgaatcac gcccgtttgt ttgatatcga
    13801 gcgccacggt gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca
    13861 acgttatccg tgcctgttga ttaccgttag aaacctgaat atcagagagc gtcgcggcac
    13921 ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca atttctgatg cgatacatca
    13981 tcccgccacc tttcaaaggt taaccggcta aacgcgccag caacaattca cccgcagagg
    14041 aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat
    14101 ccgggtcagg aagaacacct gtaccttgtt gtggccactc cacaaggcag atggcatcgt
    14161 tggcaaaata atcgcggatc cccataaact ccagctcctc gggatcggca aggcggtaca
    14221 aatcaaagtg atagaccatt aagttgtcga gcgtataggg ttcgaccagc gtataagtgg
    14281 ggcttttgac attaccctga tgacccagag cctgtaaaaa gccccggcta aaggtggttt
    14341 tacctgcgcc taaatcgcca tacagataga ttacggttgc tccatcgcag gctttcgcta
    14401 cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact cgattcatca
    14461 tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt
    14521 gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg
    14581 acacagcctg cacaggctgc atcatacggc gacagttttt gcccaagcaa tgcgccaata
    14641 ataccagaga gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc ggcatcaata
    14701 atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg
    14761 ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg
    14821 acggaacagc ctaacaaccg tgcggcctcg ctaggatgcg gcgtgatcac gcgattgtga
    14881 cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg
    14941 cgaaaattct caactttttg cagggctttt ttcccccact cttgctggcc cagaccggga
    15001 ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat agtcagttca
    15061 tgcaccatca attctggtcg tgcagtcagc agcggcgcaa tgttctcact gcgggtcagt
    15121 actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc
    15181 gccgtgccgt gatcgccacc gataatcacc agccgcccgt gatcgccttt atgcgaagtc
    15241 gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc
    15301 tcctgacctg ccagccagct atccagcccc agtgagtcaa aatgcagttg tcctgtaaca
    15361 tcccgcgctt ttccagtgag caagcctagt ttcagcgcaa taaaagtgat ggtgtgatcg
    15421 gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca
    15481 accgccacaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg
    15541 cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc taccgattcg
    15601 ggccagacaa tattcgaagc atggatctcg ccacccgcgt ttaaccatgc ttcgcgtgcc
    15661 agcgcggcct cttccggcaa cggtttgtcg ctctcctggg ccaacaacgt gacctcaatg
    15721 ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc cgccgttatt accatgaccg
    15781 cacagcacca gccagtggcg ggcgtcagga tacgccgaac gacacacctg gaatgcggcc
    15841 tcgccagcgc gaagcatcag ctcatagagt gtgagcccca gcacatctgc cgcctcgcgt
    15901 tctccgcggc ggatatcgtc ggcgtaccag acggtgtgtg gtatacttac ggggtttttc
    15961 ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt
    16021 aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc
    16081 gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg
    16141 atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattacccgg tacgctgcgt
    16201 gtaatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa
    16261 aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg
    16321 cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt
    16381 agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc
    16441 ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcgtt cttcttttta
    16501 ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggc
    16561 aaatgcgtgg cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc
    16621 gatgctcgcc gctgtatctc ttatctcacc atcgaacttg aaggggcgat cccggaagag
    16681 ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg
    16741 tggaatcgct attcgcaact cactacagaa gacgatttca gcccgcgtaa gccgctacac
    16801 gcaccggaac tcattgagtt atttgcctgg agcgaagaga agtttttaaa agtcacggaa
    16861 ggatcggcga ttcgccgtat aggtcacctg cgctggctgc gtaatatcgc cgtggcatta
    16921 ggcaatgccc cttgggatga aacgattttg acagcgctgg aaagtcgtaa aggtgagcac
    16981 ccacttctcg atgagcacat agcgtgggcg attgcgcagc aaatagagag acgaaatgcg
    17041 tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg
    17101 ttaccgcgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat
    17161 tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cggaatcttg atttttaact
    17221 tatttatcaa taggttaaaa aataccattc accgtgcgaa gtttctttga aattaagatt
    17281 acagcctgag gctgtggata actctgttta caagagtttg tcagagacaa caaaaaccag
    17341 caataacgca tcgcgtcgct gtggatattt tattgagaga aga
//
LOCUS       DADWTH010000080        17374 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.081, whole genome shotgun sequence.
ACCESSION   DADWTH010000080 DADWTH010000000
VERSION     DADWTH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.081"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..600)
                     /locus_tag="JNY02_002841"
     CDS             complement(<1..600)
                     /locus_tag="JNY02_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB9434313.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHKVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
     gene            complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNY02_002842"
     CDS             complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNY02_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBB9434314.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNY02_002843"
     CDS             complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNY02_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBB9434315.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNY02_002844"
     CDS             complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNY02_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBB9434316.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2535..4193
                     /gene="fliF"
                     /locus_tag="JNY02_002845"
                     /pseudo
     CDS             2535..4193
                     /gene="fliF"
                     /locus_tag="JNY02_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            4186..5181
                     /gene="fliG"
                     /locus_tag="JNY02_002846"
     CDS             4186..5181
                     /gene="fliG"
                     /locus_tag="JNY02_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBB9434317.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVNDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5174..5860
                     /gene="fliH"
                     /locus_tag="JNY02_002847"
     CDS             5174..5860
                     /gene="fliH"
                     /locus_tag="JNY02_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBB9434318.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQVHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            5860..7233
                     /gene="fliI"
                     /locus_tag="JNY02_002848"
     CDS             5860..7233
                     /gene="fliI"
                     /locus_tag="JNY02_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBB9434319.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7252..7695
                     /gene="fliJ"
                     /locus_tag="JNY02_002849"
     CDS             7252..7695
                     /gene="fliJ"
                     /locus_tag="JNY02_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBB9434320.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7692..8819
                     /gene="fliK"
                     /locus_tag="JNY02_002850"
     CDS             7692..8819
                     /gene="fliK"
                     /locus_tag="JNY02_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBB9434321.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8924..9388
                     /gene="fliL"
                     /locus_tag="JNY02_002851"
     CDS             8924..9388
                     /gene="fliL"
                     /locus_tag="JNY02_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBB9434322.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9393..10397
                     /gene="fliM"
                     /locus_tag="JNY02_002852"
     CDS             9393..10397
                     /gene="fliM"
                     /locus_tag="JNY02_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBB9434323.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10394..10807
                     /gene="fliN"
                     /locus_tag="JNY02_002853"
     CDS             10394..10807
                     /gene="fliN"
                     /locus_tag="JNY02_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBB9434324.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYDVRITDIITPSERMRRLSR"
     gene            10810..11175
                     /gene="fliO"
                     /locus_tag="JNY02_002854"
     CDS             10810..11175
                     /gene="fliO"
                     /locus_tag="JNY02_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBB9434325.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11175..11912
                     /gene="fliP"
                     /locus_tag="JNY02_002855"
     CDS             11175..11912
                     /gene="fliP"
                     /locus_tag="JNY02_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBB9434326.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11922..12191
                     /gene="fliQ"
                     /locus_tag="JNY02_002856"
     CDS             11922..12191
                     /gene="fliQ"
                     /locus_tag="JNY02_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBB9434327.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12200..12984
                     /gene="fliR"
                     /locus_tag="JNY02_002857"
                     /pseudo
     CDS             12200..12984
                     /gene="fliR"
                     /locus_tag="JNY02_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
     gene            13269..13892
                     /gene="rcsA"
                     /locus_tag="JNY02_002858"
     CDS             13269..13892
                     /gene="rcsA"
                     /locus_tag="JNY02_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBB9434328.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13936..14124)
                     /gene="dsrB"
                     /locus_tag="JNY02_002859"
     CDS             complement(13936..14124)
                     /gene="dsrB"
                     /locus_tag="JNY02_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBB9434329.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14287..14514
                     /gene="yodD"
                     /locus_tag="JNY02_002860"
     CDS             14287..14514
                     /gene="yodD"
                     /locus_tag="JNY02_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBB9434330.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14812..15627
                     /locus_tag="JNY02_002861"
                     /pseudo
     CDS             14812..15627
                     /locus_tag="JNY02_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15624..17318)
                     /gene="yedQ"
                     /locus_tag="JNY02_002862"
     CDS             complement(15624..17318)
                     /gene="yedQ"
                     /locus_tag="JNY02_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBB9434331.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGCIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
BASE COUNT         4373 a         4413 c         4470 g         4118 t
ORIGIN      
        1 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
       61 tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg
      121 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      181 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
      241 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
      301 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag
      361 catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca
      421 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
      481 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaacct tgtgaatctc
      541 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctt ttgccagcat
      601 actcttaccc tactttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
      661 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
      721 ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc
      781 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
      841 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
      901 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
      961 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc
     1021 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     1081 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1141 tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     1201 tgtgccttga tctcattatg cataaaggca caatgatcgc gcaaatgcaa tcataattat
     1261 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag
     1321 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     1381 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga
     1441 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     1501 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     1561 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     1621 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     1681 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctagtgtat
     1741 gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     1801 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     1861 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     1921 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     1981 caagcatagt caacaggtta cacaacctac acctgcatag acatcacttc ctgatacgcc
     2041 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     2101 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     2161 gtgcgggcga ctgtttgtgt atcgcttatg cgatcgatcg cggcgtgcag ctgcccggca
     2221 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     2281 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     2341 tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     2401 acgggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     2461 agtttgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     2521 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     2581 gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca
     2641 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     2701 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     2761 tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgc
     2821 gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg
     2881 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg
     2941 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
     3001 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
     3061 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt
     3121 ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc
     3181 tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg aaatatgcca
     3241 gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct attgttggta
     3301 acggtaatat tcacgcctag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg
     3361 aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta
     3421 atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
     3481 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc
     3541 agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca
     3601 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
     3661 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc
     3721 tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg ggcttttctg
     3781 aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg
     3841 gcggagcact gccgttttgg caacagcaag tgtttatcga tcagttgctg gctgccggtc
     3901 gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc
     3961 taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag
     4021 tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta
     4081 accaacgact gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     4141 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     4201 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     4261 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
     4321 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     4381 tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     4441 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc
     4501 ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag
     4561 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
     4621 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     4681 ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt
     4741 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     4801 atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg
     4861 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt caatgatcgc
     4921 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg
     4981 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     5041 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag
     5101 aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc
     5161 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
     5221 gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc
     5281 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggtccacg agcagggtta
     5341 tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact
     5401 ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc
     5461 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
     5521 agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc
     5581 aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     5641 attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat
     5701 gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc
     5761 tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     5821 gcaagaactc tgccgtctgg cagcactagg agtggtgtaa tgaccacgcg cctgactcga
     5881 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     5941 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     6001 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     6061 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     6121 gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc
     6181 aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg
     6241 ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
     6301 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     6361 gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt
     6421 aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt
     6481 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgaa
     6541 ggacgtgcac gctcagtggt gattgccgct ccggcggatg tttctccgct cctgcgaatg
     6601 caaggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
     6661 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
     6721 attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca
     6781 ctggtcgagc gtgccggtaa tggcattagc ggcggcggct cgattaccgc gttttatacc
     6841 gtgctcaccg aaggcgatga ccagcaggac cccattgctg actccgcgcg ggcaatcctc
     6901 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
     6961 attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
     7021 gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
     7081 ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag
     7141 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
     7201 gggctggagc gtattttccc gacagtgtca taacccagga gataacggca gatggcggaa
     7261 catggtgcgc tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgctcgc
     7321 ctgctgggtg aaatgcgtcg cggatgtcag caggcggaag aacagctcaa aatgctgatc
     7381 gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc
     7441 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
     7501 cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa
     7561 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
     7621 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
     7681 aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat
     7741 tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat
     7801 tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtggca acagataagc
     7861 ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt
     7921 tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac
     7981 cgttaaccac cgctcagaca atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag
     8041 acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga
     8101 tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg
     8161 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg
     8221 atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta
     8281 aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc
     8341 cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt
     8401 ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac
     8461 aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag
     8521 tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc
     8581 tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc
     8641 aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
     8701 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
     8761 ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac
     8821 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
     8881 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
     8941 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
     9001 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
     9061 gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt
     9121 gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac
     9181 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc
     9241 acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
     9301 taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
     9361 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
     9421 aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta
     9481 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
     9541 gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt
     9601 tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccatacc
     9661 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc
     9721 tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc
     9781 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg
     9841 aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga
     9901 aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca
     9961 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
    10021 acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat
    10081 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
    10141 tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc
    10201 tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg
    10261 atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggcactctca
    10321 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
    10381 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
    10441 cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga
    10501 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
    10561 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
    10621 agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc
    10681 gctggatatt ctgaacaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga
    10741 taaatatgac gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag
    10801 ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca
    10861 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
    10921 ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc
    10981 gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg
    11041 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca
    11101 ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt
    11161 ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta
    11221 tgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac
    11281 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    11341 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    11401 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    11461 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    11521 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    11581 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    11641 ccggcccgtt gcaggggcct gaagctgtac cgatgcgcat tttgctcccg gcctacgtga
    11701 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    11761 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    11821 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    11881 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    11941 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    12001 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    12061 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    12121 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    12181 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    12241 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    12301 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    12361 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    12421 cgtgcagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    12481 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttat
    12541 cgatcctggc gccatctcaa tatgcccgtt ttagcgcgca tcatggatat gctggcatta
    12601 ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    12661 cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc acccaccaaa
    12721 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgttg
    12781 ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt
    12841 gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc attaatgccg
    12901 ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct ggctgatatt
    12961 attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct aaggattatc
    13021 cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata tttgttttaa
    13081 gctcactcac atatcgcaac atttacttta agacaattcc aggcaaatta tacaacactt
    13141 tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg
    13201 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
    13261 ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg
    13321 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
    13381 accttgccat agcttgtgat tcacagcgtc cttcagtggt gtttattaat gaggactgtt
    13441 tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata
    13501 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
    13561 gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg
    13621 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
    13681 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
    13741 tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
    13801 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
    13861 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttcccaccag
    13921 tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc
    13981 attaaagaac cagatgccga gcgggtagtc ttccagcgca accaggtaca ttgtgccttc
    14041 actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac
    14101 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
    14161 atttcgtttt tctggcagcg ccaagcgcgc gcgagtgctg attttctcga ctgtctatac
    14221 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
    14281 aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat
    14341 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
    14401 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
    14461 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
    14521 aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt
    14581 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg
    14641 ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg
    14701 cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga
    14761 ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca
    14821 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
    14881 tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc
    14941 attctctgta gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta
    15001 caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag
    15061 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt
    15121 ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat
    15181 gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat
    15241 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct
    15301 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat
    15361 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
    15421 ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag
    15481 gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat
    15541 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
    15601 ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg
    15661 gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa
    15721 tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc
    15781 ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca
    15841 gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg
    15901 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
    15961 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga
    16021 atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg
    16081 aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac
    16141 gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggaaaggagt
    16201 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
    16261 ccgcgcacgc cttcgcttag cgtatggacg cgcgccagca caccgtcgaa atggtccaga
    16321 cgttcccagc taacatagcg actgtccata cgaatgcagc cccgcgtgtc atgttccatt
    16381 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
    16441 ttggaagagg tcaaaaatat cagcttactg tcatagagct gatactcacc atcgaggttt
    16501 ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc
    16561 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
    16621 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
    16681 tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
    16741 cgcataaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc
    16801 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
    16861 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact
    16921 aatgcatccg atacgccatt gactggcaga gtgcgccgtc ggttgagttc gatttgccag
    16981 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
    17041 agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
    17101 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag
    17161 atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat
    17221 accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc
    17281 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
    17341 ttttatgatt ctggcataac ccggcaatag cgta
//
LOCUS       DADWTH010000081        17238 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.082, whole genome shotgun sequence.
ACCESSION   DADWTH010000081 DADWTH010000000
VERSION     DADWTH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.082"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..170
                     /locus_tag="JNY02_002863"
     CDS             <1..170
                     /locus_tag="JNY02_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBB9434332.1"
                     /translation="SRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAV
                     FGGELTLVQLQ"
     gene            complement(171..362)
                     /locus_tag="JNY02_002864"
     CDS             complement(171..362)
                     /locus_tag="JNY02_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBB9434333.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(467..703)
                     /locus_tag="JNY02_002865"
     CDS             complement(467..703)
                     /locus_tag="JNY02_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBB9434334.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNY02_002866"
     CDS             complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNY02_002866"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBB9434335.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPVPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNY02_002867"
                     /pseudo
     CDS             complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNY02_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNY02_002868"
                     /pseudo
     CDS             complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNY02_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6356..6853
                     /gene="msrC"
                     /locus_tag="JNY02_002869"
     CDS             6356..6853
                     /gene="msrC"
                     /locus_tag="JNY02_002869"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBB9434336.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6950..7648
                     /gene="proQ"
                     /locus_tag="JNY02_002870"
     CDS             6950..7648
                     /gene="proQ"
                     /locus_tag="JNY02_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBB9434337.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7668..9716
                     /gene="prc"
                     /locus_tag="JNY02_002871"
     CDS             7668..9716
                     /gene="prc"
                     /locus_tag="JNY02_002871"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBB9434338.1"
                     /translation="MSMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLERSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9908..10789
                     /gene="htpX"
                     /locus_tag="JNY02_002872"
     CDS             9908..10789
                     /gene="htpX"
                     /locus_tag="JNY02_002872"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBB9434339.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10835..12208)
                     /locus_tag="JNY02_002873"
     CDS             complement(10835..12208)
                     /locus_tag="JNY02_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9434340.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12385..13176
                     /gene="kdgR"
                     /locus_tag="JNY02_002874"
     CDS             12385..13176
                     /gene="kdgR"
                     /locus_tag="JNY02_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBB9434341.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13319..13558)
                     /locus_tag="JNY02_002875"
     CDS             complement(13319..13558)
                     /locus_tag="JNY02_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434342.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13717..13860
                     /gene="mgrB"
                     /locus_tag="JNY02_002876"
     CDS             13717..13860
                     /gene="mgrB"
                     /locus_tag="JNY02_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBB9434343.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13935..14222
                     /locus_tag="JNY02_002877"
     CDS             13935..14222
                     /locus_tag="JNY02_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBB9434344.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14892..15035
                     /locus_tag="JNY02_002878"
     CDS             14892..15035
                     /locus_tag="JNY02_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBB9434345.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15048..15257
                     /gene="cspE"
                     /locus_tag="JNY02_002879"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15048..15257
                     /gene="cspE"
                     /locus_tag="JNY02_002879"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBB9434346.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15423..16232
                     /gene="rlmA"
                     /locus_tag="JNY02_002880"
     CDS             15423..16232
                     /gene="rlmA"
                     /locus_tag="JNY02_002880"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBB9434347.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNY02_002881"
     CDS             complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNY02_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBB9434348.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4554 a         4170 c         4346 g         4168 t
ORIGIN      
        1 gtagccgctc gcgattaggt gaacgcccaa aagggcaggg aattacaggt gctgatcatt
       61 tctggtttgg tcatacaccg ttgcgacatc gcgtggatat tggcaacctg cattatattg
      121 ataccggtgc tgtctttggg ggcgaactga ctcttgtgca actgcaataa ttaaaaatca
      181 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
      241 ccatggcgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
      301 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
      361 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
      421 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
      481 taatccaggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
      541 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
      601 ggtcagattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
      661 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
      721 agctgctgat tgtggtacga ccagtccgat cgcacaattt ttcagcctag ttgaggccgc
      781 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
      841 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
      901 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
      961 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa
     1021 aagcgtttac attgtcgggg gaggcatggg caataaccgc ttcatgctgc cagcgataac
     1081 ctttgttatg cgtttcggca agtttaatcc ctaagcgaga aaatcggact ttaccgatca
     1141 gggcttcaat gcctaccggg aacaaccaca gttctttgtc gcgttgccag agtcgcaggt
     1201 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtccg gcttcgcgat
     1261 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tgggacgggt aaggcgggga
     1321 tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
     1381 aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc accagggaag agatcgccaa
     1441 gtggtaaaaa ctctacttcg tcggggtaag tctctttcag ccaccggcaa acggcttcgt
     1501 tttcttcccg gtttaaggta caggtcgagt aaaccagcgt accgccagga cgtaatgcat
     1561 gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat ttcctgattg ctttcgggtg
     1621 accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
     1681 ccagcaaaat ggcatcaaac atttctggca ctgacgcacc aaacacgcgg ccaccaaaat
     1741 gtgtgagcgc aacattactg atgccacagc ggctgatatt ggcatgtaac acttttaccc
     1801 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
     1861 gcgtcgtttt tgagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
     1921 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
     1981 ttaaatgctc ggtggtacta cccaatggca atgcatcttc attgtcgcgt tcaatccaga
     2041 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaattgca
     2101 ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
     2161 gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg
     2221 tcagaaacgc gtccgggaaa taaacggcgt gttgggccac gagcatgtac cactggcaaa
     2281 aaaacaggcg cgcagtttag cataaacgct ccggcgcagg cacgccggag cagtgggcat
     2341 tatttgggaa gcgcagttcc ccattcacgc cactctttcg gttcactttc ctgcaacagg
     2401 aagtgtttgc cttcctgggc tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg
     2461 atgccgccac ggataaattg gttaaaggtg ccggttttca ctacgccgcc cgtcagacca
     2521 aagtccagac tgtaacccga tgccaaccag aagacggaat tgttacgcac caggtgttga
     2581 tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt
     2641 ccggtaaccg tacctacttc cagaccacgg aacagcacag gcgtaccgat gcctaacgaa
     2701 ccggcttccg gcgcttcaac aataatgctt aaaccatcta agtaacgcga atcagtaatg
     2761 gtggcctctt ctaattcaaa gtcgcgacga ggattgccac ggcctggttc gacgttgata
     2821 tacggctgga ggatagtatc tagatgctca acgccagctg ccgagatttg cggtgtgacc
     2881 actgagaagc gcgtaccacc gcgagcaaag gtctggacat attccggata gagcaccgcc
     2941 tttgcctgta cttcattgcg tgcggtaatc agatccagcg tctggatttg cccgatatca
     3001 ataccgagat agcgaattgg catcccgacc gccagttttc cggcatcgaa agcgtgaagc
     3061 gtaatctgcc tgccaaccgc acgggccgct gtttcggaag catacagaat tcgcttgtcg
     3121 cctttacgct gactggcgct ggcaccactg aggttatcga agctaatggc cccctttaat
     3181 gctctggaga gcggggatgc ctgtacggtc aggccactac cattcagctg aactttcgcc
     3241 ccgccttctg cctagaacac gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg
     3301 tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg taataacttc accaacttca
     3361 aatttacggt acagcactac cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa
     3421 ctcacggttg tggtgggtaa atcgctaagg ctgttctcca gcgctttttc cagattggca
     3481 tacagtggat agctggcttt catctcgcct ttatcgcccg gcagaatacg tatcccgccg
     3541 ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc catccagccc caccttcacg
     3601 tcgacacggc tgttgacgac aaatttgcta tcgcctttta ccagttctcg atgctgaggc
     3661 tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt tacgatcgat aacctggcct
     3721 acctgcacgc cgtgaagaat gagcggctga cccgcatcaa taccgtaact ttccggtgct
     3781 gtcagggtca gtgtcagaac gtccggctct tgcagcaggg ctttttcgcc cggcacaaca
     3841 acgaactctt tgcgtggctc gccatcgccg ggcaccaact caaaggtttt tccggtcagc
     3901 agggcgctga gattagcatc gctaagggat aatttcgggt tgcgtaattc tatgcgggta
     3961 ttttcacgaa gcagggtaac gacgctggga tcaacggtca tttccccagt gactttacca
     4021 ccaggattta aatccagttt agtcagctgt ccgacttcca gcccctgata cattaacggc
     4081 gtcgagtcgg cggttaatcc tgctccactc ggcagttcca gttttattat tactccacgc
     4141 tggctgtggg ccagatcttc atacagacca aaggtatctt ccgcctcggc aggtttcgac
     4201 tcttcaggtg aatcgaaggc aatcgcaccg ttaaccagtg ccgccagact ttccagtttc
     4261 accttcgcgc cactgatact gacgttggca tcaacgccgg aaacgttcca gaaacggcta
     4321 ccttttttca ccagatcggt aaaacgccgc tcgatcagga catcaatcac cacgccttgc
     4381 ttgttgggat tgatggcata gtcgtagact tttcccaccg ggatcttgcg gaaatagacc
     4441 aatgaaccgc tgttcagcga accgagatcg ggggcttgca ggtggatcat cagatcgcca
     4501 ttgtccagcc gatatttcgg ttgggtatcg agcgcgacaa agtgatcctg ctctttacct
     4561 ttacccggca tcatgccgat atagttccca ccgacgaggg cgtccagccc ggagacacct
     4621 gccaacgatg cttttggcgt catcagccag aactgagtct cttcgcgcag cgcatctttc
     4681 atatcggact tgatgctgac cttgacttca atcttacgaa gatcgtcgct gaggctgata
     4741 tcctgcactg ttccgacttc aacgccctga taacgaacag gcgtacggcc cggaacaata
     4801 ccatccgccg acataaagtc gatggtgacg gtattacccc ggtcctgata actgtcccaa
     4861 atcagccaac cggcaatcat tagcgcgatg aaaggcagca gccagaaagg tgagatacgg
     4921 cgtttatttt taatctgcgc ttcagtcgtc gaagcgggcg tttcctgact catgtgcatc
     4981 ccaaagtaag cggctgtcca gccattccac agcaagaata gtcaaaatta cggcagcgcc
     5041 aaaataaaac gcagccggtc ccatagtaaa agcgaggatc tgatcgcgat taatcagcga
     5101 catggttaaa gatatgacaa acaggtcgag catcgaccag cgtccaatcc aggtcaccat
     5161 ccgcagtaac agaatgcgtg tgcgtaaacc ttgctggcat ttaaaatgaa tgctcaacag
     5221 taaagtgaac atgacgatca ctttagtaaa cggtaccaga atactggcga taaacacgat
     5281 tcccgcaacc gcaatgttgc tacttgccag cgacataatt ccggaaagaa tcgtatcttc
     5341 ctgccggcca ccattcagat aaatgataga aataggcaat aggttggctg gtaacaacaa
     5401 aacgattgac gctaacagcg ccgcccagca tttttgcaaa ctatgacgac gacgcaggcg
     5461 tagcgggata tggcaacgcg ggcagagacc acgctgatca ggatagccgg taaaatggca
     5521 cccaagacag acacgaagtt tctcgtccct acgcgtagcg gggcgctgcg gataatatcg
     5581 ctcccacagc tcctcaacat taagatgtga caacgtcacc gtcgttaaaa tcaccaacgc
     5641 cacaaaagag aacaagccga cacccgcctg gatatgggca taatcctgta ccttatatag
     5701 aagcaacgcc aatgccgacc aggtagatat ccagcattac ccactcttta agtcgctcaa
     5761 gcatcagcag taccggacgt aaattcattc ccagtcggtt accaaaccat aaatatgcta
     5821 tggaggacac cagaatgagg ggggcaccaa taacgcagaa aaagaccatt gatcccgtta
     5881 tcgcatcgcc ctgtttggtc atttgccaga tgccttgcat gacgttggcg tcaatacgaa
     5941 tgcctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcata agcaacagca
     6001 tggtgaaggc cattgccgcc aggcgcgtta gcgacaagtc gcgcccgtcg cgaatttttg
     6061 cctgacagcg cggacaatag gcactttgat gagaatttat ctcgggcagg ctaaacagca
     6121 tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg attgccctca
     6181 ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg tgccaaatgt
     6241 aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat taacgcattt
     6301 aatgcttatt ttaataggct ggcagttcca ggtaagacaa ccaagtgatt aaataatgaa
     6361 caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg cgggagaaac
     6421 cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tacgagcgtc tcactgacgt
     6481 taactgggca ggtttttatt tgcttgagga cgatacactg gtactcggac catttcaggg
     6541 caaaattgcc tgtgtccgga tacccgtcgg gcgcggggtg tgcggcacag cggttgcccg
     6601 caatcaagtg cagcgtatcg aggatgttca cgcgtttgac gggcatattg cctgtgatgc
     6661 ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta ttggtgttct
     6721 cgacatcgat agtaccgtct tcgctcgctt tacagacgag gacgagcaag gcttacgtca
     6781 gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat tctttgcgag
     6841 cgtcgcagga taatcaacgg ataacgtagc atttactggt agcgtcatta taatgacgcc
     6901 tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca tggaaaatca
     6961 acctaagttg aatagcagta aagaagtaat cgcgtttctc gccgaacgtt ttccccactg
     7021 tttcagtgcg gaaggtgaag cgcgtccgct gaaaattggt atttttcagg atttggtcga
     7081 tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt tacgtctcta
     7141 cacttcgagc tggcgttatc tttacggtgt taaacccggc gcaacgcgtg tcgatcttga
     7201 cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca agcagcttga
     7261 agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa aacgcgaagc
     7321 tgccgcagct gctggtgaga aagaagacgc accgcgccgc gaacgcaagc cacgtccgac
     7381 cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc cggtagagaa
     7441 agcgccaaaa acagtaaaag cacctcgcga agaacagcac accccggttt ctgacatttc
     7501 agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga tggatgccac
     7561 cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta tgtctttgat
     7621 tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg agcatgtttt
     7681 ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc gctgtagaag
     7741 atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat gcgacggtaa
     7801 gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac ctcgatcagg
     7861 cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc cacaatgtgc
     7921 tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc gatgaactgc
     7981 gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag cgccgttttg
     8041 aacgttacca gtacgctttg tcggtactgg aaaagccgat ggatttcacc ggcaacgaca
     8101 cttataacct tgagcgcagc aaagcgccct ggccgaaaaa cgaggctgag ttgaacgcgc
     8161 agtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctgacagga aaaacggata
     8221 aagaaattcg tgaaaccctg acacgccgct acaaatttgc cattcgtcgt ctggcgcaaa
     8281 ccaacagcga agatgttttc tcgctggcaa tgatggcgtt tgcgcgtgaa atcgatccgc
     8341 ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg agtttgtcac
     8401 tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc aattcgatgg
     8461 tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa attgtcggtg
     8521 ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat gatgtggttg
     8581 ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct gctggtaaag
     8641 ggaccaagac ccgtactgta acgttgaccc gtgaacgtat tcgtctcgaa gaccgcgcgg
     8701 ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat attccgggct
     8761 tctatgtggg tttgacagac gatgtcaaag ttcaactgca gaaactggaa aaacagaatg
     8821 tcagcagtgt gatcatcgac ctgcgtagca atggcggtgg ggcgttgacc gaagcggtat
     8881 cgctctcagg tctgtttatt cctgcgggtc ccattgttca ggtccgcgat aacaacggca
     8941 aggttcgtga agacagcgat accgacgggc aggtgttcta taaaggccca ctggtggtac
     9001 tggttgaccg tttcagtgct tcggcttcag aaatctttgc cgcggcaatg caggattacg
     9061 gtcgtgcgct ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag caataccgtt
     9121 cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg ggttctgtgc
     9181 agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt aaaggcgtaa
     9241 caccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag aaattcgaag
     9301 ataacgcgct gccgtgggat agcatcgatg ccgcgactta tgtgaaatca ggagatttaa
     9361 ctgcctttga accggagctg ctgaaggaac ataatgcgcg tatcgcgaaa gatcctgagt
     9421 tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag cgcaatatcg
     9481 tttctctgaa ctacgctgtg cgtgagaaag agaataatga agatgatgcg acgcgtctgg
     9541 cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaag ctggatgatc
     9601 taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat atcgcactcg
     9661 atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgctcccgtc aagtaatatc
     9721 aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg taaatcagat
     9781 gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga cttgaaaata
     9841 gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact gaggtaaaaa
     9901 gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa tggtcgtttt
     9961 cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc tgatgatcat
    10021 ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca aatggatggc
    10081 attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg aacgttggct
    10141 ggtcaatact gtagcaaccc aggctcgtca ggcggggatc gctatgccgc aagtggctat
    10201 ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg cctctctggt
    10261 tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg cggtaattgc
    10321 tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga ttcagggcgt
    10381 ggtgaacacc ttcgttatct ttatttcccg tattctggcg cagcttgccg cgggttttat
    10441 gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct actttgcggt
    10501 tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca tgtggttctc
    10561 gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc gcgagaaaat
    10621 gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa ccagcatgat
    10681 ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgttcatga ctcacccgcc
    10741 gctggataaa cgtattgaag ctctgcgtac gggtgaatac ctgaagtaat atgaagtaaa
    10801 tactaaaaag cgcgtcctga tcgacgcgct ttttttatgc cctggctcgt ggctgagtga
    10861 tacgtaaacc actgacacag gcagcaatca ctgccagaat agccgcagcc atcagcgaga
    10921 cgtgcgtacc attatcacca aactgattta gcatcagcgc caccagcgcc gcgccactac
    10981 tctgacccag tagacgagcc gttcctaaca tgccactggc cccaccgcta cgttcgcgcg
    11041 gagcggaggt aataatggtg tggttattgg gtgactggaa taagccaaat ccagcgccac
    11101 ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc agaaccaggg
    11161 aaaaaagccc cgcagccatg atgaacaacc ctaaagcacc cagcaatcct gcatgtacgc
    11221 gttcaatcaa atagcctgcc agcggagcca tcaccatcgt tgctaacggc cacggtgtca
    11281 gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa aagggcaggg
    11341 aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata gaaagtgaaa
    11401 acagcgggat acgcagtaaa tccaccggta gcagcggtac gggaagagaa agctggcggc
    11461 gaataaagaa aataccaaca acaaccatta ccaccagttc cgcagcaatt aacgtcagcg
    11521 attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt aacgcgttca
    11581 tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccattg ggtggcagaa
    11641 aacgcatcgc cagaagcagg gcgataatac ctaacggtac gttgattaaa aataaccatt
    11701 tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca gaagagacgg
    11761 caacaataaa cgagtttatg cccatccctc tccccagaaa acgttgtgga tagatcaggc
    11821 ggataagtgc ggtattaacg ctcatcaacg ctgcaccgcc gaaaccttgt atgacacgcg
    11881 caagggtgag catttgcagc gaatcagaaa gggcgcagaa cagtgaagac aacagaaaaa
    11941 cgaccagacc gcatttataa atacgtcgat agccaaacat atcgcccaga aacgaaaacg
    12001 agagcaggga gatgacaatg gcgatttgat aggcgttcac tacccagatg gaactggctg
    12061 gtgtggcatg aaggtccgtg gcgattgttg gcagggcgac gttggcgatt gcgccgtcaa
    12121 ggacagccat cgaaatacca atcacaatgg ttaatatcgc accgtatcgc tggggcaatg
    12181 gcaggccgtc ggcctgaact tttggcatag gaattttata tctttggtga ataatcaaca
    12241 ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa ctaaccggca
    12301 aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc tgatttttat
    12361 aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca gcctgattct
    12421 gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga gcgcgaaata
    12481 gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta tcgcttttta
    12541 cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa atattccctg
    12601 accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt aattcgtagc
    12661 gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca cctcggcgca
    12721 ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa tttgcgcatg
    12781 tattcacgga ttgggcgtcg taatccgctg tacagcaccg cgattggtaa ggtactgctg
    12841 gcatggcgcg atcgcgatga agtgaagcaa attcttgagg gcgtggagta taaacgcagt
    12901 accgagcgga ccatcaccag tacagaagcg ttattacccg ttctggacca ggtgcgcgag
    12961 caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat tgcggtaccg
    13021 gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc gacgttgcgt
    13081 ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc gcgcaaaatt
    13141 tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc tgtcggaata
    13201 tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg cgttatatta
    13261 aacgcgcttg ctataagaat attttactca tgagtgagaa tctggttatt tattaccctt
    13321 aaccattatc gaccacgata ttgcttttac gtaacagtgg gcaatctgtt atccccaaga
    13381 aaccactttt agtgtgcaag tattgtaccg tgctggtgcc tctggcagtc agataggtac
    13441 attgcaaacc taatcctgcg gcattctctt tgcttccaat caaaacgcca tatccgctga
    13501 gtaataatcc tatccatacc agtgctatca gcataactgt gcgaatgatg aatcgcatta
    13561 caatctcttc tctttttatg ttcgcttaat cgtagcggca atatgcgctg aagcaagcta
    13621 ctcattccga aaaagcacga atatcgacat agttaggcgc tgtttaacta acgcatgcta
    13681 gtttaatggc ataaggtagg tgaagcggag attggagtga aaaagtttcg atgggtcgtt
    13741 ctggttgtcg tggtgttggc ttgcttgctg ctttgggcgc aggtattcaa catgatgtgc
    13801 gatcaggatg tacaattttt cagcggaatt tgtgccatta accagtttat cccgtggtga
    13861 tatcattttt tcagcactag attttcttcc tctccagcag tggtagaata gcgcctctac
    13921 tcagaggtgg tgaaatgaac gaagttgtaa attcaggcgt gatgaacatt gcgtctttgg
    13981 ttgtatcggt ggtggttctt cttatcgggc tcatcttgtg gttttttatc aatcgtgcca
    14041 gttcacggac taacgaacag attgaactgc ttgaggcgtt gctggatcag caaaaacgtc
    14101 aaaatgcact gttacgtcgt ttgtgcgaag caaacgaacc agagaaagca gataaaaaga
    14161 ccattgagag tcaaaaatcg gttgatgacg aagatattat tcgcctggtc gccgaacgat
    14221 aatccttcct caatcttgag taaccccgtc gttgaagggt tactcaattc atcctgatta
    14281 tacgcactgt catggtgcaa ctgcttcaaa ttaaaactct ggtctgactt caaataatta
    14341 tcatattatt tatttgcgta acgggatatc agaaaaagat attttcctca ttaatttatt
    14401 aggatgttta catcagattt gtgattaagc gtggtattat ttattagcga aacgtttctc
    14461 tcttgatttt tttgctcact catcaatttt tcttatttta aatttacaat ccctttgggg
    14521 attgacttct ctttagggta attgatagcc gttaactgac tgttttatga gaaaaagtga
    14581 tataactttt tattcattgc atagcagaaa atgtgatatt gcacgcacta tgtaataact
    14641 tctccctctg gcctggaaca actgaactta ttgaactatg ttagaaacta cgccagttta
    14701 agtatctgcc tgaactggca aggttaagca caatgatata tcggcgcgta ttccgttgca
    14761 taagtgtgca aaaaaagtgg aagacgtatc gagatttgtg cgtctgatcg agacatgttt
    14821 aaaaatggct tgccataatt aacgttgtat gtgataaaag atttcgggtt aaacgaggta
    14881 cagttctgtt tatgtgtggc attttcagta aagaagtcct gggtaaacac gttgacgttg
    14941 aataccgctt ctctgccgag ccttatattg gtgcctcatg cagtaatgtg tcagttttat
    15001 ctatgttatg cctgcgggcg aagaaaacaa tctaaggaat ttttcaaatg gcaaagatta
    15061 aaggtcaggt taagtggttc aacgagtcta aaggttttgg cttcattact ccggctgatg
    15121 gcagcaaaga tgtgttcgta cacttctccg ctatccaggg taatggcttc aaaactctgg
    15181 ctgaaggtca gaacgttgag ttcgaaattc aggacggcca gaaaggtccg gcagctgtta
    15241 acgtaacagc tatctgatcg aatccactga tctgaagtgt gaatacgctt caatctcgct
    15301 ataaagcctc gtcgaatgcg aggcttttta ctatgcttta tcttcgctcc tggcgttcgg
    15361 atatttgcca gccacgtgat tcgcgttaca cttgcggcct ttagtatcct gccggagttg
    15421 tcatgtcgtt ttcctgtcca ctttgccatc agcctctttc gcgtgaaaaa aacagctata
    15481 tctgtcccca acgacatcag tttgatatgg cgaaagaagg gtatgtcaat ctgctgcccg
    15541 ttcagcataa acggtctcgt gatccgggcg acagcgcgga aatgatgcaa gcacgccgcg
    15601 cattcttaga tgccggacat tatcagccgc tgcgtgatgc aattgtcgcc caactgcggg
    15661 aacggcttga tgaaaaggcc acggcggtgc tggatattgg ctgtggtgaa gggtattaca
    15721 cacacgcatt tgccgatgcg ttgcccgaaa tcaccacgtt tggcctggat gtctcgaagg
    15781 tagcgataaa agcggcggcg aaacgctatc cgcaggtcac tctttgtgtc gcttccagcc
    15841 accgtttgcc gttttccgat accagtatgg acgccataat acgtatttac gcgccgtgta
    15901 aagcagaaga attagcacga gtagtgaagc ccggcggctg ggtcattact gccacgccgg
    15961 gaccgcgaca tttgatggag ctgaaggggc tgatttacaa tgaagtacat cttcatgcac
    16021 ctcatgcaga acaactggaa ggttttacat tacagcagag tgcggagttg tgttatccga
    16081 tgcgtcttcg cggtgatgaa gccgtcgcat tattgcagat gacgccgttt gcctggcgtg
    16141 cgaagccaga agtctggcaa acactggcag caaaagaagt gttcgactgc cagacggact
    16201 ttaatattca cctctggcag cgttcttatt aaccgtggaa gtgcgtccag aggatctgga
    16261 cgccgatgcc gatcagcacc agcccgccga gaatttccgc ttttttccca ataattgagc
    16321 cgataaagcg accaaccatc atccctaatg tcgacataat caaggttgca caaccaatgg
    16381 ccaatgcggt cgcgataatg ttgacctgaa ggaaagcaag accaacaccc acagccatgg
    16441 catccaggct ggtggcaatc gcggtggtta ccagtagcca gaaaccgtgc cgacggcgcg
    16501 gctcttcatc ttcatcatct gagccacgaa aaccctcaat aatcattcgc ccgccgagga
    16561 atatcagcag cacaaacgca atccagtgat tccattcaag gacaaaccgg ctggctaaca
    16621 tgcccattcc ccagccgatc agcggcgtca gggtttcgac ggcaccaaaa ataaggccgg
    16681 ttcgcaatgc ttcagaaaat ttcggtttat ggaggggggc acctttaccg attgatgcag
    16741 caaatgcatc catcgacata ccaaacgcaa gaagaacagt agcagtgata ttcatgacaa
    16801 cgtcctgacc ggggtatcca taaaacacat catcgccccc agcaaacatg catacatgaa
    16861 caacaagccg cgatgggttt tcgcgttcat gcaggcatag cgattgatgt gcatatggtc
    16921 tcgcctgact gaaagtcttc agtccgtacg tgccacgttt tgcaacgagt atgttgacac
    16981 gtacatttcc ggaatctgga aatcggctac tccccaaatg agggcgcaac cttaacatat
    17041 tttgagaata taaaacaact gttggaatat attatttcat tgctttattg ataacgattt
    17101 tcatttatgt ttatatatca atgaaacgtt aaaataaata acaaataagg gaagagataa
    17161 tatagcctaa gctatgtctg attattaaaa atacactaaa catagctttg gctaaattca
    17221 taagttattg agttgata
//
LOCUS       DADWTH010000082        17183 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.083, whole genome shotgun sequence.
ACCESSION   DADWTH010000082 DADWTH010000000
VERSION     DADWTH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.083"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(329..673)
                     /locus_tag="JNY02_002882"
     CDS             complement(329..673)
                     /locus_tag="JNY02_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBB9434349.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="JNY02_002883"
     CDS             805..2715
                     /locus_tag="JNY02_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBB9434350.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="JNY02_002884"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="JNY02_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBB9434351.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="JNY02_002885"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="JNY02_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBB9434352.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="JNY02_002886"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="JNY02_002886"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBB9434353.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="JNY02_002887"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="JNY02_002887"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBB9434354.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNY02_002888"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNY02_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBB9434355.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="JNY02_002889"
                     /pseudo
     CDS             complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="JNY02_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(9210..9761)
                     /locus_tag="JNY02_002890"
     CDS             complement(9210..9761)
                     /locus_tag="JNY02_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9434356.1"
                     /translation="MRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDR
                     LAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKEL
                     HSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSK
                     YGIYEQEGLVAPSQEPQDVKKAV"
     gene            9987..10214
                     /locus_tag="JNY02_002891"
     CDS             9987..10214
                     /locus_tag="JNY02_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9434357.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
     gene            10208..10639
                     /locus_tag="JNY02_002892"
     CDS             10208..10639
                     /locus_tag="JNY02_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434358.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKVLEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNY02_002893"
     CDS             complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNY02_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBB9434359.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(10768..10866)
                     /locus_tag="JNY02_002894"
     CDS             complement(10768..10866)
                     /locus_tag="JNY02_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBB9434360.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNY02_002895"
     CDS             complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNY02_002895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBB9434361.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            12107..12361
                     /gene="yoaF"
                     /locus_tag="JNY02_002896"
     CDS             12107..12361
                     /gene="yoaF"
                     /locus_tag="JNY02_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBB9434362.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(12383..12730)
                     /locus_tag="JNY02_002897"
     CDS             complement(12383..12730)
                     /locus_tag="JNY02_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBB9434363.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNY02_002898"
                     /pseudo
     CDS             complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNY02_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            14063..14884
                     /gene="nimR"
                     /locus_tag="JNY02_002899"
     CDS             14063..14884
                     /gene="nimR"
                     /locus_tag="JNY02_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBB9434364.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(14841..15287)
                     /locus_tag="JNY02_002900"
     CDS             complement(14841..15287)
                     /locus_tag="JNY02_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBB9434365.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            15457..15561
                     /locus_tag="JNY02_002901"
     CDS             15457..15561
                     /locus_tag="JNY02_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434366.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNY02_002902"
     CDS             complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNY02_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBB9434367.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNTGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(16108..17166)
                     /locus_tag="JNY02_002903"
     CDS             complement(16108..17166)
                     /locus_tag="JNY02_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBB9434368.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQHVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
BASE COUNT         4406 a         4175 c         4358 g         4244 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtaacagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg ggcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgattagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgcctggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 ctgtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat
     9721 tctccattca ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg ggcataaaaa
     9781 tgcgctctgc atttttgctt tctttttcac ctgtcatgct caatctctct gttatataaa
     9841 gaattttaca gaattctttg atttgcattg gttaaaataa aaatgtgcat agctcttgaa
     9901 atgcgcattt ttctgcgata tcgtttttcc cgatagcggt agccggtatc agttaccgaa
     9961 aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc
    10021 agataaaggc tgctgttcgt atgaagggga agacactatc tcaattatcc agagaggcag
    10081 gcttaaagcc tgacactatg cgtaacgtat ttcgctgcca tataccacga tacgaagcaa
    10141 tcattgctag atatttggat atggaaccag cccagatttg gccttctcgg tatatggaga
    10201 aaacatcatg ttaacccatt gggtaacagc gcaagaatgc gtaggaatgt catgctttcc
    10261 taaacatgag gtgagtattc gccgcaatct tgaaaagctt gcagccaatc atccagaact
    10321 acgtcgcaag cgtacgggtt caaaggtttt ggaatttcat atatcagttc taccagcggc
    10381 agcacgtgtt gagttgttat taaaatctga gcaaatagaa acaagccagg ggtattttga
    10441 aatcgcccgc cccacgctgg aagcgcacga ttacgatcgt gaggcactgt ggagcaaatg
    10501 ggataaagcc agcgattccc agcgcagact tgctgaaaaa tggttgcctg cggttcagac
    10561 tgcagacgaa atgcttcagc cgttattggt gcgattgtcg tgctatttat ctacaggaag
    10621 attaaaagtt aacgcttaaa ttgcacaaag gctgcacaca ggcagccttt gctatttttt
    10681 agaggtgacg ttaccaccat cccagctcag tgccgagtac gacaataatc gccacaccca
    10741 tcataataat cgttgttttt ttcatcttta tgctcccggg gcagcatagc cgccaataaa
    10801 aaaccatgct aaaaatacga ccgctgtaat gaagattaca accggaaaaa taataccgat
    10861 tcgcatgtta tcaccaatca aaaggatgtg aatacgcctc aggaatgtag cgctggatgc
    10921 gcgacaaaag gtgttttctg tttgtgtttt ttctcctgat acatcgcaat atcggcagca
    10981 tgcagggcac tgtctgcatc ggttgtttca ggatcgactt ctactatgcc aagactggca
    11041 ccgggataat acaaatcaac atcacctaag tgatattctc cacgtatttg ctgttgaata
    11101 cgttctcgca gcgacgaaat atccgcattc tcgttgttta gtgaaacaac caaaaactca
    11161 tcaccaccga gacgaccaat aacttcgcca ttttgctgga gcgtattaag gcgctcgcca
    11221 acctgaatga gaaacagatc gccactatta tgaccaaaac gatcattgat taatttgaag
    11281 ttatccagat cgataaacgc gatcattatc ttatggttaa gatgccgggc gagggaaaac
    11341 agcgtcgtca gattttcaaa aatcgcccgc cgattcggta gcccggttaa cgagtcggta
    11401 tacgattgcg caatcagcgc agcattggct tcgtgcagct gttcaaccag tgcctctttt
    11461 tgaatatatt gtgcaatcag tccggcgaat aactgtaaaa cctgttccgc gcgttcactc
    11521 cactggcgct tctcactgct ggcggcgcaa agcgtgccat agaatgatcc atccggtaag
    11581 tgaattggcg tgctcagaaa tgtggtgatg ccaagattgc gtgccgcaat acagtcgccc
    11641 caacggtcag ggacttcatc gctgaaaaag cagttttcat caatggcgcg tttgcataac
    11701 gagtaatccc acgagacggt aaaattctcc gggatgtgca ttttctggct gttacgggca
    11761 aacataatat gctgcaggcg cgcttcgaca tccactttgg tcaggtaggt tgattccata
    11821 tcagtgacca tttccagcat ctccagaagc tgtcggacca gactttccag tgactgttct
    11881 ttggcaaggg attgcgagac gcgggcgata atctgatctg acacggaaca actccaggat
    11941 acggcgggta agtgctgatt atttaatctt tgcaggcatg ataaaacatc atcaacaaag
    12001 atgaaacaag acgtaatatg ttttgagtga atgtaataaa aatagtgctg caaaacaaaa
    12061 cgataccgcg atactctaag cttggataaa tatgtgagat caataaatga aaattatcag
    12121 ttttgttctg ccttgcttgc tggtcctggc tggttgttca accccttctc agccagaagc
    12181 acctaaaccg ccgcagattg gtatggcaaa tccggcgtcg gtctattgcc agcagaaggg
    12241 cgggacgctc attccagtgc agacagcgca aggggtcagc aacaattgca aattaccggg
    12301 cggtgaaacg attgatgaat gggcattgtg gcgacgggac catccggctg gtgaaaaata
    12361 accgccggat gccgtactaa aatcacaagc tacgtagcca gtcggccagc accagcgcat
    12421 ggttctgcgt ggtgtttttt gctgcgtaga gcagggtcag cggctgtttt ttggcgatgt
    12481 ccgccagccg ctttccttct tgctcgtgtt gcgccagttc tgcgcgatat tgctcgcgaa
    12541 aggttgcaaa atcgacgact tcgccgtgaa aggctttgcg cagttccgtt gacggcgtga
    12601 tttctttatc ccactcatca agggctaaat cggttttttt gataccgcgc ggccagaggc
    12661 ggtcgaccag tacgcgataa ccatcgctct gttcagccgg atcataaacg cgtttgcact
    12721 gaatattcat cgtgtttgtt ctccctttca cgcaagattt gtcacgtcac tatagcaccg
    12781 gatattagcg aatttctttg tctctcccgg cgcaaagtcc gaaaatcgcc atcagtagtg
    12841 aaagtattgc aacacccatc agtggtacgg accagttacc gttagcatcg tgtattttac
    12901 ccattagcgg cggcccacag gctgccagca aatacccgac ggattgtgcc atccccgaga
    12961 gtgccgccgc ctgatgcgca gaacttgccc gaagaccaat gaacgtcaac cccagtatca
    13021 ttgttgcgcc ggaaccaaaa ccgaaaagca gagtccaggt gatcgcgtgc gctggcataa
    13081 agcagagccc aaccgcgccc actgcgcaca tcaaggcaac gaacgctgca ataccacgct
    13141 ggtctttcac atgatgtaag aaaagtggga tcagcaaacc gggagctgct gtggctagtt
    13201 gcagcaaacc atgcagtgaa cccgcctgtg cttcgctata gccgtgactg atgaggatcg
    13261 ccggaagcca gccaataatc acgtaataga ccagtgagtt gatcccaaga aacaatgtta
    13321 cctgctaggc aagtggcgaa cgccagatac cccgagtatg taaggcgcgt gaggtactca
    13381 aatttgcatg ttgctgactt cgccactgtg gcagccataa aaaaagagcc agcagaggaa
    13441 aacacatcag catgaacaac gcgccttgcc agccaaaacc gttcaaagcc agcggcacga
    13501 ccatagccga tcccagtgcc gctgcagctc ccattgtcag ggaatatgcg ccggtaagtc
    13561 tggcgacgga atgagggaaa tcgcgtttaa ttaatcctgg cagtaagaca ttgcctaatg
    13621 caatcccacc gccaatgacc gctgtaccgc caaataataa gtaaggcgaa gggagagaac
    13681 gaattgcgat acctgcacag atcaaaagta acgcggcaaa caggctacgt tccataccaa
    13741 aacgtcgcgc caccggggca gccagaggtg agattagcgc aaaggccaat aatggcaggg
    13801 tggttaataa gccggtttgc gctgtcgtca gcgagtaagc ggaacgaatc gtatccagta
    13861 acggcgctgc gccggtaaag gtgacgcgta atgttgtggc aatcatcaga atgccagcga
    13921 taaggacaat cctgtttttg ccgcttaatg aagttgaaca ggtcatatgc ttctcattgt
    13981 tacaaacgag gctgtacctt accgttttta ttgttatgtt gaatcaagct aaaatgacaa
    14041 attatcgcta agttcggaca aaatgatgca caggttgaat ctcaatggct atgaacccga
    14101 ccgacaccat gaagccgcgg tcgcattttg tattcacgcc ggaacggatg agttaaccag
    14161 tcctgtacat cagcaccgca aaggacagct gatactagca ttacatggtg caattacctg
    14221 tacggtggaa aatgctttgt ggatggtgcc gccacaatat gcggtttgga taccgggcgg
    14281 ggtgaagcat agcaatcaag tgacagctaa cgctgaactc tgttttctgt ttatcgaacc
    14341 cagcgcagtg acaatgccga caacatgttg cacattgaaa atatcgccct tatgccggga
    14401 attaatttta accctggcaa acaggacgac gactcagcgt gctgagccga tgacccgcag
    14461 attaattcag gtcctgtttg atgaactccc tcagcagcca caacaacagc tgcacctgcc
    14521 cgtttcttct catcctaaaa tccgcacgat ggtggagatg atggcgaaag ggcccgtcga
    14581 gtgggggtca ttggggcaat gggctggctt ttttgcgatg agtgaacgca atcttgcccg
    14641 actgatagtg aaagagacgg ggttgagttt tcgccagtgg cgtcagcagt tacagctgat
    14701 tatggcgctt cagggattag tgaaaggcga tactgtacag aaagttgctc atacgctggg
    14761 gtatgattca acaacggcct ttattactat gtttaagaaa ggcctagggc aaacgccagg
    14821 ccgatatatt gcgggattaa ctactgtttc cccacaatca gcgaaaccag acccgcggca
    14881 ataagcgggc caaccggtac gccgcgaaac agcgctacgc ctaaaacagt gcccaccagc
    14941 agcccagcaa ccagttgcgg ctggctgccc attaacgtca cgccgcgccc acccagccag
    15001 gagacaataa cgcctactgc aattgccacc agcgatttcc agttcagaaa cgagtgaatc
    15061 aaggttgagg gcggtagcgt cccgctggcg atgggggcca taacgccaat cgtcagaatg
    15121 attatcccga tacttagccc ctgtttttca atccacggga aaaaggtgct taacggtgtg
    15181 acgcggacaa tgatcaacac cagaattgaa acggcgacag tggtgttatg actgataaag
    15241 cccagcgcag ctaacccgag caggatcagc aaagtgacat caaacatcgt ggtttccttg
    15301 ccaaaaaata ataagcctgc ttactttacg cttgctgcag gaaggaagta gaaaatcttt
    15361 acgttttttg cataaaaaat caggcgaact gtcatcaaac tgccatttgt gcacgctaac
    15421 aataacgtga aaacagctat ctcccggggg catgctatga acgatcaaat gttcgtcgag
    15481 acactgatta tcacatcatc gttttttgct attgcggttg tgctggtttt gtccgttctg
    15541 cttattgaac ggataggcta gctacgcagt gcggcggaaa ttgaccagtt ctggttgcgc
    15601 aatgcgcaaa taatccgcgg ttttcaaaat aacggattta tccagcatcc ctgtattgaa
    15661 ggcgatttca tcaaatcgtt cgaagagtag agggtcggca accagtttga gttttggatg
    15721 gaagctgaaa ggggggatcg cgccgaagac acagcctgtg agttcatcaa cttccgccgg
    15781 gctggccaac gaagcgcgta atccgcctat atggctggcg agttggctca gatcggcctg
    15841 ttgatcggct gcgaggatcg ccagaacatg ttgatttacg ccattgcctt tgactttaca
    15901 taccagtgcc ttagcaccct ggcctagagc ggtgccacga atttctgaca ccgcttcaca
    15961 tttacctacc gcttcatggg taacgacgcg gaagttagca ccttcttgtg ataataaagc
    16021 gattaactgc tgatgcgtca cgcttccttt agccatttca gtcatttcac gctcctgaga
    16081 ttacaagcaa acaaccacag aaggttatca tgaacggctg tttttgttct gcttattgac
    16141 atataaacgc tcatcggaag ctttatacgc atcatgtaac gtaatcgcta aatccccctc
    16201 ctgatgaccg aggttgtcat ttatttgctt taacttgtcc atgtcaattg caataaacat
    16261 caccgaagaa ccggattgca ccagtttctg caaccgctgc tccagttcag gggttaaaat
    16321 tttgcgatta tacagtccag tcatcgcatc actaatattt tctcgcgaca cattttggta
    16381 taaacggaag tgcatccgga ccatattcag caaaattaac gccgtcaaaa tccagaacag
    16441 aacgcttttc cacgaagacg tgataaagta aagaatatca atggataacg agacacgaat
    16501 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
    16561 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
    16621 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
    16681 cacaatccct tttagctgcc ctgcgacata taccggcgtc agcaaactca tcacgtgctg
    16741 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
    16801 aatatctttg cgatcgatgc caatacttcc tgactgcagg aaggaccagt tattcatcgc
    16861 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
    16921 atagtgaaat ttctcttcgg ctatcccaag acctgaaccg taatcatcct ggtggcggtt
    16981 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
    17041 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatggg cagtcaggtt
    17101 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
    17161 cgacacttcc gactgctcag cag
//
LOCUS       DADWTH010000083        17121 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.084, whole genome shotgun sequence.
ACCESSION   DADWTH010000083 DADWTH010000000
VERSION     DADWTH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.084"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(467..1600)
                     /locus_tag="JNY02_002904"
     CDS             complement(467..1600)
                     /locus_tag="JNY02_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434369.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(1770..2108)
                     /locus_tag="JNY02_002905"
     CDS             complement(1770..2108)
                     /locus_tag="JNY02_002905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434370.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(2698..3639)
                     /gene="fabY"
                     /locus_tag="JNY02_002906"
                     /gene_synonym="yiiD"
     CDS             complement(2698..3639)
                     /gene="fabY"
                     /locus_tag="JNY02_002906"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBB9434371.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3684..4121)
                     /gene="dtd"
                     /locus_tag="JNY02_002907"
     CDS             complement(3684..4121)
                     /gene="dtd"
                     /locus_tag="JNY02_002907"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBB9434372.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4118..4990)
                     /locus_tag="JNY02_002908"
     CDS             complement(4118..4990)
                     /locus_tag="JNY02_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBB9434373.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4984..5583)
                     /gene="yihX"
                     /locus_tag="JNY02_002909"
     CDS             complement(4984..5583)
                     /gene="yihX"
                     /locus_tag="JNY02_002909"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBB9434374.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5682..6467)
                     /locus_tag="JNY02_002910"
     CDS             complement(5682..6467)
                     /locus_tag="JNY02_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB9434375.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6501..7397)
                     /gene="yihV"
                     /locus_tag="JNY02_002911"
     CDS             complement(6501..7397)
                     /gene="yihV"
                     /locus_tag="JNY02_002911"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBB9434376.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7565..8461
                     /gene="yihU"
                     /locus_tag="JNY02_002912"
     CDS             7565..8461
                     /gene="yihU"
                     /locus_tag="JNY02_002912"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBB9434377.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8485..9363
                     /gene="yihT"
                     /locus_tag="JNY02_002913"
     CDS             8485..9363
                     /gene="yihT"
                     /locus_tag="JNY02_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBB9434378.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQPGDIVDEMMAKRR"
     gene            9380..10621
                     /gene="yihS"
                     /locus_tag="JNY02_002914"
     CDS             9380..10621
                     /gene="yihS"
                     /locus_tag="JNY02_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBB9434379.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLFDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10759..11661
                     /locus_tag="JNY02_002915"
     CDS             10759..11661
                     /locus_tag="JNY02_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBB9434380.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11860..13896
                     /gene="yihQ"
                     /locus_tag="JNY02_002916"
                     /pseudo
     CDS             11860..13896
                     /gene="yihQ"
                     /locus_tag="JNY02_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            13942..15327
                     /locus_tag="JNY02_002917"
     CDS             13942..15327
                     /locus_tag="JNY02_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9434381.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTLYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15370..16773
                     /locus_tag="JNY02_002918"
     CDS             15370..16773
                     /locus_tag="JNY02_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9434382.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            16841..>17121
                     /gene="ompL"
                     /locus_tag="JNY02_002919"
     CDS             16841..>17121
                     /gene="ompL"
                     /locus_tag="JNY02_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBB9434383.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         4267 a         4339 c         4116 g         4399 t
ORIGIN      
        1 gggatattta tttgcataat ctgaatggtg tagccgaaaa acgtcagcat cctaacagca
       61 ccaggattta aggtgaaatt aatctttcat cacagaccgg tcagtactgc caccaaccgg
      121 tctttacttg ctcatcgtta taattttgtt ggtaacagtt cgggaaacaa ggaattcctt
      181 ccggaacacc cccggcatca tccgatagca ttgacgtcaa tcatcagcgc gacgatgagt
      241 ttttttaatc gcatcatagg gtggcgacgt tcagcacgat tacgggtcat agcaaaactc
      301 ctgagcaggc ggccactatg ccgctttcac tggagttctg aatactggta ctttctgtat
      361 ggcttcgcca tgcccctcgc ggctaagtac ctgcacacat ctctctttgt atgtcatttt
      421 tgcaatgcat tgtccgaatt ctgataatcc taatgagttt tactcgtcag actcccatcc
      481 ctacggtata cgagttatat tcatgaatga aatcgacatt tttaaaaaac gaatcttgta
      541 aaacatcagt gttgatatta ccttgcaatc caacattgaa tttgttagag acatgaagaa
      601 ctcgaatatc aaaaaggttt tttaacatca ttaattcttc gggtgttaat gaatcgcaac
      661 taaaatatcc ctgttgatca aaaagatttt ctatttctcc actatcggcg aggcgttttt
      721 caaggagtgt ttgcaacatc gagttgccaa ggacaatgga tatcgtgcga tctttcgcag
      781 atttacaccc catcacaacg gcaacattcg tatccccctc aagagtttca tttatcgccg
      841 tcgctaaaat ggtggtcaaa cacgcaaacg gaactgtatt tctttcgtga tttaatttat
      901 ctaccagttc attccattta tcaaaagccg ggctatattt gttgcgcaca gaaatatccg
      961 actggttaaa caattcagcc atgcgatttt ttatcatctc gatatccggc gaaccagaaa
     1021 gactttgcgc ataatacggt cctgatgatg ttgcatacat taatgccaag cccacacctg
     1081 tcgccatgat gggcgcagcc acggttttgg caagggcgac gctgcgatgg ctatgcgctt
     1141 cacaggctaa aggaacatat tccactgact gcggtaattc cttacatgcc tccctcatca
     1201 ttgcggtatg ctttacaaac aacggttttt cactgcccaa aaaaactttc tgcttaattg
     1261 cactaagaaa acgcaaatca aaaataattg tttttggttt attatttttt ggcacatttc
     1321 ctattatccc gctaagaacc ttccttgctg catggagatt tttttgcaca gcacataaca
     1381 caattgatga agccttctcc actgcattat tgattttaat tttaaaatcc gcatgatata
     1441 cgttgacggg caatgcaagt atatctggac ctacatcttt tgctgagtat ttattggtga
     1501 tcgacggacg tgaacccacg agaaaactta gctgcaaatt atgctctata ttatcagctg
     1561 tttttgtggt gtggttaaac atagagatgc cacctttcat tctttcacac atatcctcaa
     1621 ctttttcact tcgttcttgt ggcaatttgg ctttaacatt aatttgatga tttttacccg
     1681 tgcattcctt aactacaagg ggaatgttat ctaaagtgcc atacaggcta ataacgtcac
     1741 caggattttt atcatgtaag taagtttgat taatacttga agcattcaca aagagggtca
     1801 atattataac tgtttataac ttcacctaca tcaagtgtgt agtgcttact ctggagactg
     1861 ccatccttat ttaattgtat atcaatcaat atatccccgg actttgtcac catatctttg
     1921 ccataaacca tttgcatagc catttcgagg agatttttat ctaactgatt gcgggcgttg
     1981 agtaagcgat taccaaataa tttatatact gttcccgtca ttgccagcgc tgcgctttct
     2041 ggtactttgc gtttccacag tgaggtttct ctatttttaa acaggtcata ttcattatta
     2101 ctcgacaaac catgcttcag ttcgttaaca caatcgcaca gttgctcctc accttccatt
     2161 ttaaggcaaa gtgaaaattg atttaatggc gtttacttcg gtgatttatt actcattagt
     2221 tgtttttcta aattaacaac agaataactt tcttgtttaa tgagattaat attggcaacc
     2281 attatagcct ccttcccacg gtttaagagc aaacatacaa cataatgatt cataacatac
     2341 agagcagtaa atgaatgata ctgacaatgt atgccagccc attatctttg cataatgttt
     2401 gtgagctata gattttaaaa atatttatga aaaaagtatg gcaccagagg gggttttgac
     2461 tatcaaaaat cgctcaaacc tgaactaaga tgatgagtta agcattatct gcaataattg
     2521 ccactcagct ctacaaataa tatttaaatg ctcctgaaca gtgatgtagc aagacacgta
     2581 tggtgccaac gaagaataaa aatttataaa gttcttattc aatataaccg gaatagtcag
     2641 ggactgatgt aaatgaccgg aatgaatgag agaggcagca ccgcccctct ccataagcta
     2701 ctcttcttcg ttcccgccct cttcatacgg gccaaatggc ttcgcgggca gaacgatata
     2761 cgtgccttca aacactgcac ccggcgtctc gtcgccaaag atttcgacct gcatctgcac
     2821 ccgtgctttt cgtccgcgcg ccagacggtc gagatcgccg cttaaggcac caaggtcggc
     2881 taccgcatgg ggtttaccgc taatcggttt gctgtagcgg atatgcgcat ccgccagaat
     2941 aatcgttccg ccgaggtggc gttcacgcag catcagccag ataagccccc aaccggtgag
     3001 cgtcgccagc gagaataaac tcccggcaaa cagcgtatgg tgcggattct gattgccggt
     3061 ttccggcatg gtggtgataa atttttgccc ggtatattgt tgaatacgca cgcccatttt
     3121 ttcactaagc gggatatgtt cgtaccacgc ctgttgcagc tgcgcgcacc agtcgccgcg
     3181 atgcaaaata tcatccagag tggcgacggg cttaatcatc aaaaaatggc gaatcggcgt
     3241 ggtggttggc gttgtgattt ctccctgatt aacaaacccc agcttggcga aaaactccac
     3301 cgcgtcttca cgggcgctac aggtcacgcg cttaacgcct tcctgacgcg ccaccgactc
     3361 cagggtcatc gccatcagcg ttcctaaccc tttgtcctgc acgtccgggt gaacggccat
     3421 aaagcgaatg gacgcttcat tgtcggcatt aatatacaat cggcctaccg ccaccagatt
     3481 accctgctcg tcgacgacca tctggtgatg cgccatcgca tcccacgcgt cgcgttccga
     3541 accttttggt tgatgcaggg gcttacgcaa catttcccag cgaaactgat agtaacgctc
     3601 taactcttct tctgtttgtg gaacctgaag gtgatacata gcgatactct ctcttgtttc
     3661 ccgtgacaac cctggaagct ggctcatacc tgcaaccaga atgtcacggg gccatcattg
     3721 accagcgata cctgcatatc ggcagcgaag cgtcctgttt gcgtgttcat ctcttgctgg
     3781 cggcagcgtt cgacgaaata gtcatataac gcctctgcgc gatccggtga tgcacctttg
     3841 gagaaacttg ggcgcatccc ccgttcggta tctgcggcga gggtaaactg ggaaaccacc
     3901 agtacactgc cgcccgcctg ttgcacgttg agattcatct tgccttcggc atcgctaaag
     3961 atgcggtagc cgagcacacg ctcgcacaga cggtttgctt tctgttcgtc gtcatccttt
     4021 tcgacaccca ataacaccaa aagtcccgcg ccaatttcgc ccgtcacttc tccctccacg
     4081 gtgacgctgg cacgggttac gcgttgaatt aatgcaatca tggttcgtcg tcttcttctt
     4141 gttcagctgc ttgttttagt ttgcggtatt ccccgagagt gacagtaatt tccgcgccaa
     4201 gcaagacgat acaccatgtc cagtagaccc aaacaaagag aatggggatc accgccagca
     4261 caccgtaaat gagctgatat gacgggaaca tggtgatata aagcgcgaaa cctttctttc
     4321 ctgcttcgaa cagaagtgcg gcgacaaacg cgccgacaat cgcgtcgcgg ttaggtacgc
     4381 ggatggtagg aacaatgctg tacagcaacc agaaggagat ccacgacaac agcaacggaa
     4441 aaatacgcag cacgttgtcg atgacagtat tgagatcgct cgcccagcgc agggagagca
     4501 aataggaact gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt aaaatcatcc
     4561 agtacacggc gaacgagtaa atttttggtc gcgctcgttt actgcgccag atggtattca
     4621 acgcgctatc gatggagtac atcaacaata acgcggtgac gatcagcccg cacgccccaa
     4681 cggcggtcat cttgttggaa ttagcaacaa attgttcgat ataccgctga ataacatcgc
     4741 cagtagcagg cagaaagttg gcaaaaataa agtgacgtaa ctgaatgctg acgtcggaaa
     4801 acatgggaaa agcggcgaaa agcgcaaaaa caacggcaac cagcggcact aatgagagca
     4861 acgacacata ggcaaggtta cctgccaggg ttgtcatgtt gtcctcatca atacgttgcc
     4921 agagtagttt tagccaggcc catagtggac gggtacggtg cctggctttg tcctgaatgg
     4981 tttttagcat aacaccttcg cgaaatagtc cgggatggtg gttttatctt tcaccagaat
     5041 actggtaatg cctagctgat tagctccttc tatattatcg gcgttatcgt cgaaaaagac
     5101 cgtatcgctg ggtgaaaaac cttccgcctg caaaacatgc tggtaaattc gtgcttcagg
     5161 tttgcgcatc cccagatctt gcgacagata gatatggtca gcagcatcac gaatttccgg
     5221 gtattcttcc ggccagaagg tggtatgcag gcggttggta ttggaaagca ccaccacgcg
     5281 atgcccctgc tcacgcagtt tatgcatgat ggcgatcact tccgggcgca gcgcaacaaa
     5341 caccgcctgc cagccgtgag agaactgctc gtagcttagc ggtagagcca tctcatgaca
     5401 cagcgcctct gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat gaaacgcctc
     5461 ccccatatga aaactcttct taagcgatgc cagcggaata cgcgttaaat cgctccaggc
     5521 tcccagcaca cggttaaagt cgatatcgac aatcacatta cctaaatcaa agatatagag
     5581 catttttgcc tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga tgggtttggc
     5641 tatgtggcaa ggtatgaagc caggttaacg gtcacaaaaa tctacgcgtc ttcctgggga
     5701 agaatgattt caatttggct gtcttgtaag cgtgatttta gcgcagcggg cggcagctcg
     5761 tcggtaaaga gcgcagtgac ctgtgccacg ttaccaattt caaccgccgc cgaagcatga
     5821 tacttagtgt gatcggcgac cagcagaata tttctcgcgt gcgccatcat cgttttcacc
     5881 acgttagctt cgtttacatc aaactccatc aacgcgccat cgctctcaat cgccccaacg
     5941 cttgttacca gataatcagc acgaaaatca gccacaaagg acgccgctga agggccaatg
     6001 atcccgctat tatgagagcg caacgtaccg ccgggcacca tcacttcaaa gcgcgggttg
     6061 tggtaaagaa tatgcgccac acgcaggctg ttggtgatta tccgcaaatg attatggtta
     6121 agtaacgccc gggcaacatg ctcaacagtc gtaccaatgg tgataaatat tgttgaacca
     6181 tcaggaatat agtctgccac cgcttcggca atcgcttttt ttttctcggt ttgcgaaacc
     6241 tcacgctgct cgaacgccgt attaacgacg ctggaagccc gacccgcgcc accgtgatgg
     6301 cgcgtaatca ggccttgctc gcttaattta cgaatatccc ggcggaccgt ctgcgtggag
     6361 acatccagca gatttgccag ctcatcaata ttcatatacc cacgctcggc gatcagcatg
     6421 aggagttggt cgtgccgcgg gttaccggtt agttcggtaa ggctcatgaa aattcctcga
     6481 aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa aagatcgggt ttgatcacag
     6541 tcagggatcc cggctcgtcc accgggacgt gtgcatttta acgccgctac accgctggcg
     6601 aagcggactg actccgctaa atccccactt gttgccagcg ccaccgccaa agcgccgtga
     6661 aaaacatcac ccgcacctgt ggtatctacc acatcaactt tgaaggccgg ttgatgctga
     6721 cgcccaccat tttccagcca gtcgcagcct gcgctaccct gggtcacata gacatgacca
     6781 tttgtgagcg tttgtgcctg ttttagcgca ctggccatct ctttcacgcc cgttaagcgc
     6841 gccagacccg gttctgaaaa ggccgcgtga tcgcttaatg ccaccagctc actgatatcc
     6901 tgcggcgtaa tgtccccgtc cagaacggtc atcacacccg cctgacgggc cagggtgaag
     6961 gcttttttag cgccgtcgtg ccagcgtaca tctgccagca caacatccca ctgagagaaa
     7021 tcaatttcct ccaaccactc tgcgtcaggc agcagatccg ggctggggta gttaatgatt
     7081 atccgctcgc ctttggtatc caccatgatg gcggattgcg aagatttcgc ctggttatac
     7141 cgtttggtgt aacgggtgtt aacgccccag gattccagct ctgctagcag gctgttgccc
     7201 gcgtcgtcat cacctacgcg accaataaaa tcgacctgcg cccccagcct tgccgccgca
     7261 accgctgccg tcgccgctgg cccgccacca acttccgtat aatttctcgc cacgtattta
     7321 ccgctctccg tcggtaaccc ttccacgtaa tagatgcgat ccatcacggt tatacctaca
     7381 caagcaacac gaatcatggt cattccttaa acattttggg atgcgttcat tttcatttca
     7441 ccaattgttt aaaaagtgac ggctgtcaat tttttgacga taaattacaa atgcgatcaa
     7501 aaacagacaa gcaaagcatg tcataaatga caaaaagtga catcactgta ttcaggagag
     7561 ggttatggca gcaatcgcgt ttatcggttt aggacaaatg ggttcgccaa tggcgagcaa
     7621 tttattgcag caagggcatc aacttcgcgt cttcgatgtg aacgccgaag ctgtgcggca
     7681 tctggtagac aaaggtgcga ctcccgccgc caacccggtg caggccgcta aagatgccga
     7741 atttatcatt accatgttgc cgaatggcga tctggtgcgc aacgtgttgt tcggtgaaaa
     7801 cggcgtttgc gaaggcttat ctaccgatgc gctggtcatt gatatgtcca ccatccatcc
     7861 gctgcaaacc gataaattga ttgccgatat gcaagccaaa ggcttcagca tgatggatgt
     7921 tccggtaggc cgtacttctg caaatgccat taccggtact ctgttactgc tggctggcgg
     7981 caccgctgaa caagttgaac gtgccacacc gatcctgatg gcgatgggca gtgagttgat
     8041 caacgctggc ggtccgggca tggggatccg cgttaagctc atcaacaact acatgagcat
     8101 cgcgctcaat gcgctttcgg cagaagccgc cgttttgtgc gaagccctga atcttccctt
     8161 cgatgttgcc gtcaaagtga tgagcggtac cgccgccggt aaaggtcact tcaccacttc
     8221 ctggccaaac aaagtcctca gcggagatct ttctcccgcc ttcatgatcg atcttgccca
     8281 taaggatctt ggcatcgccc ttgatgtcgc caaccagctg catgtgccaa tgccgctggg
     8341 ggccgcctca cgggaggttt atagccaggc gcgcgcagcg ggtcgcggtc gccaggactg
     8401 gtccgccatt ctggaacagg tccgtgtcag tgccgggatg actgccaaag taaaaatgta
     8461 acgactggat aaaggaataa atgaatgaat aagtacacca tcaacgacat tacgcgcgca
     8521 tcgggcggtt ttgccatgct ggcggtcgat cagcgcgaag ccatgcgcat gatgtttgcc
     8581 gcggctgggg cacccgctcc ggtagccgat agcgttttaa ctgatttcaa agttaacgct
     8641 gcaaaggccc tctcgcctta tgcctcggcg attctggtag atcaacaatt ctgctaccgc
     8701 caggtggttg agcaaaacgc gattgccaaa agttgcgcca tgattgtcgc cgccgatgag
     8761 ttcattcctg gcaacggtat tccggtcgat agcgtggtta ttgaccgcaa aatcaatccg
     8821 ctacagatca aacaagacgg tggcaaagcc ttaaaactgc tggtgctgtg gcgtagtgat
     8881 gaagatgcgc agcaacgtct ggatatggtg aaagagttca acggactgtg ccactcacac
     8941 ggtctggtaa gcatcattga gccagtcgtc cgcccaccgc gtcgtggcga taaattcgat
     9001 cgcgaacaag cggtcatcga tgccgccaaa gagctgggcg acagtggcgc tgacctctac
     9061 aaagttgaaa tgccgcttta tggcaaaggt ccgcaacaag agcttctctg tgcttcacaa
     9121 cgtctgaatg accatatcaa tatgccatgg gtgatcctct cttccagtgt cgacgaaaaa
     9181 ctgttcccgc gtgccgtacg cgtggcaatg acggcgggcg catcgggatt cctggcaggc
     9241 cgtgcagtct gggcatcggt cgtcggttta ccagacaacg agctgatgct ccgtgacgtt
     9301 tgcgcaccga aattacaaca acctggcgat atcgtcgacg aaatgatggc taaacgccgc
     9361 taagatgagg ataaaaggaa tgaaatggtt taacacccta agccacaacc gttggctgga
     9421 acaggaaacc gaccgcatct ttgattttgg taaaaattcc gtagtgccga ctggttttgg
     9481 ctggttaggc aataaagggc aaatcaaaga agagatgggc acccatctgt ggatcacagc
     9541 tcgtatgttg cacgtttatt ccgttgctgc ggcgatgggt cgacctggcg cttacgcgtt
     9601 ggttgatcac ggtattaaag ccatgaacgg cgcactgcgt gataaaaaat atggcggctg
     9661 gtatgcctgc gtgaatgacg agggcgtggt ggatgcctcc aaacagggct atcaacactt
     9721 ctttgctctg ctgggtgccg ccagcgccgt cacaacgggt cacccggaag cgcgcaagct
     9781 gttcgattac accattgaga ttatcgagaa atatttctgg agcgaagaag agcagatgtg
     9841 cctggaatcc tgggacgaag ccttcagcaa aaccgaagag taccgcggcg gcaatgccaa
     9901 tatgcacgcg gtggaagctt tcttgattgt ttatgacgtc actcacgaca aaaaatggct
     9961 ggatcgcgcg attcgcgtgg cttccgtgat tatccacgac gtcgccagaa ataatcatta
    10021 tcgcgttaac gaacacttcg atacccagtg ggatccgctg ccggattaca acaaagataa
    10081 cccggcgcat cgcttccgcg cgttcggagg tacaccaggc cactggatcg aatggggccg
    10141 tttaatgctg cacatccatg ctgccctgga agcccgttgc gaacaaccac cagcatggct
    10201 gctagaagat gccaaaggtc tgtttaacgc caccgtgcgc gatgcctggg cacccgatgg
    10261 cgcggacggg attgtttata ccgttgactg ggaaggaaaa ccggtggtcc gcgaacgtgt
    10321 acgttggcct atcgtcgaag caatgggtac ggcctacgcg ctctacaccg tcactggcga
    10381 tcgccagtat gaaacctggt atcaaacatg gtgggagtac tgcattaagt acctgatgga
    10441 ctatgaaaat ggttcctggt ggcaggagct ggatgcggac aataaggtca taaccaaagt
    10501 ctgggacggc aaacaggata tttatcacct gctgcattgc ctggtgatcc cgcgtatccc
    10561 gttagcgccc ggcatggctc cagcggttgc agcgggtctg ctggatatta atgcgaaata
    10621 actgatctga aaatagggtg cgggttttcc cctcacccta accctctccc caaaggggcg
    10681 aggggaccgt ccggtgcagt ttttgggttt cacatcagcc tcacgggagg ctgtatgtcg
    10741 ttaattaagg tgccttgtat gcaaatcacc aatatgcatt gcagtggaca gaccgtttca
    10801 cttgccgcgg gggattatca cgcgaccatt gtgaccgtcg gtgctggtct ggctgaatta
    10861 acctttcagg gttgtcattt agtcatcccg cacaaacccg aagagatgcc gctggcgcat
    10921 ctgggtaaag tgcttattcc gtggccgaac cggatcgcca acggctgtta ccgttatcag
    10981 gggcaggaat atcagttacc cattaatgaa catagttcaa aagccgctat tcacgggctg
    11041 cttgcctggc gagactggca aattagtgaa ttaaccacca ccagcgttac cctgacagct
    11101 tttctgccgc ccagttacgg ctatccgttt atgctggctt cacaagttgt ttattcgctc
    11161 aacgcacaca cgggtctttc cgttgagatc gcaagccaga atatcggcac tgtcgcagcg
    11221 ccttatggcg tgggtattca tccttattta acctgcaact taacatctgt tgatgaatat
    11281 ctgttccagc ttccggcaaa ccaggtttat gccgtcgatg aacacgtaaa tccgacaacg
    11341 cttcaccacg ttgatgagct ggatttaaat ttcacgcagg cgaaaaaaat agccgcgaca
    11401 aaaatcgacc acacctttaa aaccgctaat gatttgtggg aaatgaccat tacccatcca
    11461 cagcaggcgc tttcggtaag cctttgttcc gatcaaccct gggtgcaggt ttatagcggt
    11521 gaaaaattac agcgtcaggg actggcagtt gaacctatga gttgcccgcc taacgccttt
    11581 aattcaggta tcgacttatt attactggaa ccaggaaaac cgcaccgcct gttttttaat
    11641 atttacggac agagaaaata acttgtattg attagcagtc aaaatcattg aagaacattt
    11701 cactcgctaa aggcaacatt atgtatcttc aatttattaa gttaaaaaca gctcagcttc
    11761 tgtaaagttt ttatatatca gttttatgag aatcatttta cataagcctg taataacagg
    11821 aaggcaacaa tctcgttctc aactctatgg agattagtta tggatacgcc acgtccacag
    11881 ttattagatt ttcaatttca tcagaataac gacagtttta ccctacattt tcaacaacgt
    11941 cttattttaa cccatagcaa agataatcct tgtttatgga ttggctcagg tatagcggat
    12001 atcgatatgt tccgcggtaa tttcagcatt aaagataaac tacaggagaa aattgcgctt
    12061 accgacgcca tcgtcagcca gtcaccggat ggttggttaa ttcatttcag ccgtggttct
    12121 gacattagcg ccacgctgaa tatctctgcc gacgatcagg gccgtttatt gctggaacta
    12181 caaaacgaca accttaacca caaccgtatc tggctgcgcc ttgccgctca accagaggac
    12241 catatctacg gctgcggcga acagttttcc tacttcgatc tgcgcggcaa accgttcccg
    12301 ctatggacca gtgaacaagg cgttggtcgc aacaaacaaa cctatgtcac ctggcaggcc
    12361 gactgcaaag aaaatgcggg cggcgactat tactggactt tcttcccaca gcctacgttt
    12421 gtcagcacgc agaagtatta ctgccatgtt gataacagtt gctacatgaa cttcgacttt
    12481 agcgccccgg aataccatga actggcgctg tgggaagaca aagctacgct acgttttgaa
    12541 tgtgctgaca catacatctc cctgctggaa aaattaactg ccctgctggg acgccagcca
    12601 gaactgcccg actggattta tgacggggta acgctcggca ttcagggcgg gacggaagtg
    12661 tgccagaaga aactggacac catgcgtaac gcgggcgtga aggttaacgg catctgggcg
    12721 caggactggt ctgggatccg tataacctct tttggcaagc gcgtgatgtg gaactggaag
    12781 tggaacagcg aaaactaccc gcaactggat tcacgcatta agcagtggaa tcaggagggc
    12841 gtgcagttcc tggcctatat caacccgtat gttgccagcg ataaagatct ctgcgaagaa
    12901 gcggcacaac acggctatct agcaaaagat gcctctggcg gtgactatct ggtggagttt
    12961 ggcgagtttt acggcggcgt tgtcgatctc actaatccag aagcctacgc ctggttcaag
    13021 gaagtgatca aaaagaacat gattgaactc ggctgcggcg gctggatggc tgacttcggc
    13081 gagtatctgc ccaccgacac gtacctgcat aacggcgtca gtgccgaaat tatgcataac
    13141 gcctagcctg cgctgtgggc gaagtgtaac tacgaagccc ttgaagaaac aggcaagctc
    13201 ggcgagatcc tcttctttat gcgcgccggt tctaccggta gccagaaata ctccaccatg
    13261 atgtgggcgg gcgaccagaa cgtggactgg agtctcgacg atggcctggc gtccgtagtc
    13321 ccgacggcgc tgtcgctggc aatgaccgga catggtctgc atcacagcga cattggcggt
    13381 tacaccaccc tgtttgagat gaagcgcagc aaagagctgc tgctgcgctg gtgcgatttc
    13441 agcgccttca cgccgatgat gcgcacccac gaaggtaacc gtcctggcga caactggcag
    13501 tttgacggcg atgcggaaac tatcgcccat tttgcccgta tgactaccgt tttcaccacc
    13561 ctgaaacctt atctgaaaga ggccgtcgcg ctgaatgcga agtccggtct gccggtcatg
    13621 cgcccgctgt tcctgcatta cgaagacgat gcacatacct attccctgaa atatcagtac
    13681 ctgttaggcc gcgacattct ggtcgctccg gtacacgaag aaggtcgcag cgactggacg
    13741 ctctatctgc cggaggataa ctgggtacat gcctggacgg gtgaagcgtt ccggggcggg
    13801 gaagttaccg ttaatgcgcc catcggcaag ccgccggtct tttatcgcgc cgatagcgaa
    13861 tgggcggcac tgttcgcgtc gttaaacagc atctaatcac atccgcccgt ggggaaactc
    13921 atgggcaaag ggagaacatc gatgagtcac atcacaacgg aagatccagc aactttacgc
    13981 ctgcccttta aagagaaact ctcttacggt attggcgacc tggcctctaa catcctgctg
    14041 gatatcggta cgctttatct tttgaagttt tataccgacg ttctggggct gccaggcacc
    14101 tatggcggca ttatcttttt gatttcaaaa ttctttactg cgtttaccga tatgggtacc
    14161 ggcattatgt tggattcccg acgcaagatc ggtccaaaag gtaagttccg tccttttatt
    14221 ctgtatgcgt cattcccggt caccttactg gcgatcgcca actttgtcgg cacaccgttt
    14281 gatgtcaccg gtaaaacggt gatggccact attctgttta tgctttacgg actgtttttc
    14341 agcatgatga actgctccta cggcgcaatg gtcccggcaa tcaccaaaaa ccccaacgaa
    14401 cgcgcctcac tggcggcatg gcgtcagggc ggcgctacgc tgggcctgct gctgtgcacg
    14461 gtgggattcg tgccagttat gaatcttatc gaaggtaatc agaaacttgg ctatatcttc
    14521 gccgccacgc tgttttcact gttcggcctg ctgtttatgt ggatctgcta ctcaggcgtg
    14581 aaagagcgtt atgtcgaaac ccaaccaacc aatccggcgc aaaagcctgg cctgttgcaa
    14641 tctttccgcg caattgccgg taaccgccca ctgttcattc tgtgtattgc caacctctgc
    14701 accttagggg cgtttaacgt caagctcgcc attcaggtct attacaccca gtacgtactt
    14761 aacgatccca tcctgttgtc gtatatggga tttttcagca tgggctgtat tttcatcggc
    14821 gtgttcctga tgcctggcgc agtcagacgt tttggtaaga agaaggtcta tatcggcggc
    14881 ctgctgattt gggtgctggg cgatctgctc aactatttct tcggcggcgg ctcggtcagc
    14941 tttgtggcgt tctcctgcct ggcgttcttc ggctcagcgt ttgttaacag cctgaactgg
    15001 gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg ttcggaaggc
    15061 acgctctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc tggtttcttc
    15121 cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc tgaccacacc
    15181 attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt agtcaccatt
    15241 gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat tgtggaagag
    15301 atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg ggcgttataa
    15361 ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg ggaaaaaatc
    15421 gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac tctgtatctc
    15481 ctcaaacttt ataccgatga attagggatg cctgcttact atggcgggat tatctttctg
    15541 gtcgcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact cgactcgcgg
    15601 aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc agttccggcg
    15661 gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt taaaacgacg
    15721 attgccaccg cgctgtttat gatgtttgga ctttcataca gtctgatgaa ctgctcgtat
    15781 ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct cgcggcttac
    15841 cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat tccgttgcag
    15901 tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt ctccattggc
    15961 ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt ggacacagcg
    16021 ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg taatccacca
    16081 ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat caaactggcg
    16141 attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc gtggatggga
    16201 ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac tgtaaaatgt
    16261 tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg tgatatactg
    16321 aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt cgcctttttt
    16381 ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt agattacggt
    16441 gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac cttctttcgt
    16501 aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca aattggttat
    16561 gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct catttttatc
    16621 tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta cacactcaat
    16681 gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa agagatggcg
    16741 acagaaaaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat ttccatcctg
    16801 gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga ttaatgcaat
    16861 aattttatta tcttctctga cctctgcctc ggtatttgct ggcgcatacg tcgaaaacag
    16921 ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg gttataactt
    16981 cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag aagatgaact
    17041 aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa ccgataaatt
    17101 aaccatccag cccggtggct t
//
LOCUS       DADWTH010000084        17091 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.085, whole genome shotgun sequence.
ACCESSION   DADWTH010000084 DADWTH010000000
VERSION     DADWTH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.085"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(464..1660)
                     /gene="hemY"
                     /locus_tag="JNY02_002920"
     CDS             complement(464..1660)
                     /gene="hemY"
                     /locus_tag="JNY02_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBB9434384.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1663..2868)
                     /gene="hemX"
                     /locus_tag="JNY02_002921"
     CDS             complement(1663..2868)
                     /gene="hemX"
                     /locus_tag="JNY02_002921"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBB9434385.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQTMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(2890..3630)
                     /gene="hemD"
                     /locus_tag="JNY02_002922"
     CDS             complement(2890..3630)
                     /gene="hemD"
                     /locus_tag="JNY02_002922"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBB9434386.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3627..4568)
                     /gene="hemC"
                     /locus_tag="JNY02_002923"
     CDS             complement(3627..4568)
                     /gene="hemC"
                     /locus_tag="JNY02_002923"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBB9434387.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4955..7501
                     /gene="cyaA"
                     /locus_tag="JNY02_002924"
     CDS             4955..7501
                     /gene="cyaA"
                     /locus_tag="JNY02_002924"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBB9434388.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENSFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7541..7861)
                     /gene="cyaY"
                     /locus_tag="JNY02_002925"
     CDS             complement(7541..7861)
                     /gene="cyaY"
                     /locus_tag="JNY02_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBB9434389.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7909..9222)
                     /locus_tag="JNY02_002926"
     CDS             complement(7909..9222)
                     /locus_tag="JNY02_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBB9434390.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9219..10697)
                     /locus_tag="JNY02_002927"
                     /pseudo
     CDS             complement(9219..10697)
                     /locus_tag="JNY02_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10886..11089
                     /locus_tag="JNY02_002928"
     CDS             10886..11089
                     /locus_tag="JNY02_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB9434391.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11126..11950
                     /gene="dapF"
                     /locus_tag="JNY02_002929"
     CDS             11126..11950
                     /gene="dapF"
                     /locus_tag="JNY02_002929"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBB9434392.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11947..12654
                     /locus_tag="JNY02_002930"
     CDS             11947..12654
                     /locus_tag="JNY02_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBB9434393.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12651..13547
                     /gene="xerC"
                     /locus_tag="JNY02_002931"
     CDS             12651..13547
                     /gene="xerC"
                     /locus_tag="JNY02_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBB9434394.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13547..14263
                     /gene="yigB"
                     /locus_tag="JNY02_002932"
     CDS             13547..14263
                     /gene="yigB"
                     /locus_tag="JNY02_002932"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBB9434395.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14347..16509
                     /gene="uvrD"
                     /locus_tag="JNY02_002933"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14347..16509
                     /gene="uvrD"
                     /locus_tag="JNY02_002933"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBB9434396.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16656..17048)
                     /locus_tag="JNY02_002934"
     CDS             complement(16656..17048)
                     /locus_tag="JNY02_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBB9434397.1"
                     /translation="MRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGPMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3957 a         4428 c         4537 g         4169 t
ORIGIN      
        1 ttgatgctgt ttttagtttt aacggcaatt aatatatgtg ttattaattg aatgaatttt
       61 atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact caggaagtta
      121 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      181 ttcccgattc tctgtggcat aataaacgag tagatgctca ttccatctct tatgttcgcc
      241 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      301 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
      361 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgc
      421 atcagaaagg tcccggaggt gctttcctcc gggtgagaag gaactactgt ggcgggttat
      481 tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca
      541 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
      601 ccgcgcggaa ggcgagcgat gcttcctgcc attcaccgtg cttcatcagt gactgaccca
      661 atgtgctcca caacagcggg cgatcaccga cgtttttgat ttgctggcgt agcacttttt
      721 ccagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg cgatcgtcat
      781 actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg tcacattcaa
      841 taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc cggctttggt
      901 ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga tccatcaggc
      961 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
     1021 ccattgatgg gatgatatcc agcagagaac tccatgcccc tgtgcggata tacgcctgtt
     1081 ccgccagacg taatacttca ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     1141 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     1201 gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt
     1261 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttcag
     1321 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcacct tccgccagtt
     1381 tcagcagcgc ctgttcggtc tgcttacgcg cacggcgacg cttacgtccg acaaaccatc
     1441 cacgggtgtg cgcgccagtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     1501 ctaccatcgc cagaatcaat atgatcgcca ggcccgtgac gctggtttcg atattgtagt
     1561 tgtcggtctg gatcagcaca taaccctgat gaccggcaat catcgggcca accactatcc
     1621 ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc cttgcggcgc
     1681 ggctgccgga gtatcacctt gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg
     1741 tttagcttcc gttgtccccg ctgccggttg tgccagcagg ttacgcacgc gagtctgcat
     1801 cagtttttcc agcatcgtct ggctttgcag ggtttccgga agatccatcg agatattttg
     1861 ctggcttaac tggtccacct cgtcgaggaa cgctttggtg gtggcatcat cagtatcgta
     1921 gtaagcacgt acccaggtgg agacgttctc cagcgcctgg cgataagtct cttcctgatg
     1981 acgcggtaca gcttgtgctg cgaccagcag gcgagagcga atattttcgc gcagatagat
     2041 atcctgattt ggcgctaaca gcggtacggc ggtgtcatca cgacggcgaa tcgtaatgaa
     2101 gttgtccata aagttctgcc agcttttttg cagattgata cgccattcgc tgatggaact
     2161 ggaaagctct tcaccgtctg aatccatcgg cgaaccatcg ctgtcattat cggccagacg
     2221 caggttatct acctgatttg aaagctgatt aagcttaagg atgatgccgt cataatccac
     2281 ctgcgatact gcagaaaggc tggcgatatc atcggtaatt gcccgacgaa cggtaatcag
     2341 actcgggtca ttcatatccg ccaggctggc gtctgcactt ttcagcaacg ctgcagaggt
     2401 cgtgacgtcc tgatcgctcc acagcttccg tccggcgagt ttcaccagaa aatcggcctg
     2461 agccagcagc caggttttag catcgctgcc ggaaatggtg gcgatctttt gttggacttc
     2521 atccaactgt tttgccagcg tttcttgctg acgattcgcc tgcttaagct gcgcagcttg
     2581 ttgcttaata atgccttcca gctcggcttt ttggctctcc tgggcttttt gcaatgccgt
     2641 cagttggtta gccagggcat cgctggtggc ggtctgattg acggcctgtt gtttacccca
     2701 gccatacaaa ccgacacccg ccgccagagc aatagcgata gccaccgcgc tgagaatcaa
     2761 tgcagtattg ttcttactct ttttttctgt tgcgacaggt tgtgacgtgg tgtccacggc
     2821 ctccctggtc tcttcaacca cggcggaggt tttttcttgt tccgtcatta tggcttcctg
     2881 ttatgagagt tattgtaatg cccgtaaaag cgcatcgttg tcagcgttat cggcgacctt
     2941 aatgtcttgc cagcccagtt cccgggcgag tttcgccaaa cgctcactga cgaccaatag
     3001 tcgacagtgt agtaaccagt gctcacgata ccattgtggg atcagcgacc agagttgctg
     3061 caacatttca ccgctggtaa caacgaccat cgtcacctcg cgggattgcc agcgcatcgc
     3121 ttcttctgca ccatcgtaat ggattgcgca tcgttgataa cattcacaaa aagtgacctc
     3181 agcaccgcgc accgtcaggg tatccccaat tagctcacga ccgccattgc cacgtaatat
     3241 cagcgcacgt ttgcccgcaa tattttgtaa ttcaggtaat tgtagcaaga cttcgctgat
     3301 ttcccgatcc tgtgggtaga gaatcttctg tccgcttacg gtatgtagcg ccagtgcggt
     3361 ggtgcgtcca atggtgaaat aagtaggtag ttggggccat ttacgatctt gctgatgcag
     3421 ctgtgattgg gcaaaagcaa ccgcgtgttg cgagagggca aacaacagat cgctctcccc
     3481 cagcgctgcc agttgatcag caagttgcgg taattgtcga cccggagaaa actcaatcag
     3541 cggaaaatgc caggccacct gccccagtgt gcgcagacgg ctcactaact cttctccagc
     3601 gggagacggg cgggtgacca ggatactcat gccggggcct ctccgttata gacttcagcg
     3661 aggatctcgc gcgcgccgtt attcagtagc tcttctgcca gcgaaatccc catttgttcg
     3721 gcatcttgcg gcgcaccgcg gcgttcaccg cgaataatct gcgaaccgtc cggcgcgccg
     3781 accagcgcac atagccagat ttcgccatca ataagctcgg cgtagctacc aattggcacc
     3841 tgacatccgc cttcgagacg ggtattcatg gcgcgttctg cggtaacgcg cagtgcagtt
     3901 tcgtggtgat tcagcgcggc aagcagctcg cgagtgcgtg tatcatcaag gcggcattca
     3961 ataccgactg caccttgtcc taccgccgga agagaaatct cgggtggtaa cgcggcgcga
     4021 atgcgggact ccagacctaa acgttttagt ccggctacgg caagaatgat ggcatcgtat
     4081 tcgccgttat ctagtttgct caggcgagtg ccgacgttgc cgcgcaggga gcggataatc
     4141 agatccgggc ggcgttcagc cagttggcac tggcgacgta aactggacgt cccgacgata
     4201 ctgcctgctg gtaacgcatc cagactgtca tagttattgg acacaaaggc atcgcgagga
     4261 tcttcacgct cgcaaatagt gaccagcccc agaccttgcg ggaattcaac cggcacatct
     4321 ttcattgaat gtacggcgat atcagcgcga ttctccagga gcgcgacttc cagctctttt
     4381 acaaataagc cttttccgcc tactttcgcc agcggcgtat caagaatcac atcgccgcgc
     4441 gtcaccatcg gtaccagttc aacgaccagg cccggatggc tcgccatcaa cttgtctttg
     4501 acatagtgtg cctgccagag tgcaagtggg ctttggcgtg tggcaattct taaaacattg
     4561 tctaacatgc ttgttaccgt cattatcatc cgtggtccat cctaacatcc ttgccagagt
     4621 gatgtcagtg ttgtggtgaa acgtagacgc ctgcgcaaac cgtaaaatga ggtctggcag
     4681 tggatcctga caggcgtttc acgccgtcgt aataaggaat ttacagagaa taaacggtgc
     4741 tacacttgta tgtagcgcat ctttctttac ggtcaatcag caaggtgtta aattgatcac
     4801 gttttagacc attttttcgt cgtgaaacta aaaaaaccag gcgcgaaaag tggtaacggt
     4861 tacctttgac atacgaaata tcccgaatgc cgcgtgttac cgttgatgtt ggcggaatca
     4921 cagtcatgac gggtagcaaa tcaggcgata cgtcttgtac ctctatattg agactctgaa
     4981 acagagactg gatgccataa atcaattgcg tgtggatcgc gcgcttgctg ctatggggcc
     5041 tgcattccaa caggtctaca gtctactgcc gacattgttg cactatcacc atccgctaat
     5101 gccgggttac cttgatggta acgttcccaa aggcatttgc ctttacacgc ctgatgaaac
     5161 tcaacgccac tacctgaacg agcttgaact gtatcgtgga atgtcagtac aggatccacc
     5221 gaaaggtgag cttccaatta ctggtgtata caccatgggc agcacctcgt ccgtagggca
     5281 aagttgttcc tctgacctgg atatctgggt ctgtcatcaa tcctggctcg atagcgaaga
     5341 gcgccagtta ctacaacgta aatgcagcct gctggaaagc tgggccgcct cgctgggtgt
     5401 ggaagtcagc ttcttcctga ttgatgaaaa ccgcttccgt cataatgaaa gcggcagcct
     5461 ggggggcgaa gattgtggct ccacccagca tatactgctg cttgacgaat tttatcgtac
     5521 tgccgtgcgt ctcgccggta agcgtattct gtggaatatg gtgccgtgcg acgaagaaga
     5581 gcattacgac gactatgtga tgacgcttta cgcgcagggc gtgctgacgc caaatgaatg
     5641 gctggatctc ggtggcttaa gctcgctttc tgctgaagag tactttggtg ccagcctttg
     5701 gcagctctac aagagtatcg attccccata caaagcggtg ctgaaaacac tgctgctgga
     5761 agcctattcc tgggaatacc cgaacccacg tctgctggcg aaagatatca aacagcgttt
     5821 gcacgacggc gagattgtat cgtttggcct cgatccatac tgcatgatgc tggagcgtgt
     5881 tactgaatac ctgacggcga ttgaagattt cacccgtctg gatttagtac gtcgctgctt
     5941 ctatttaaaa gtgtgcgaaa aactcagccg tgaacgcgcc tgtgtaggct ggcgtcgcgc
     6001 agtgttgagc cagttagtga gcgagtgggg ttgggacgaa gctcgtctgg caatgctcga
     6061 taaccgtgct aactggaaga ttgatcaggt gcgtgaggcg cacaacgagc tgctcgacgc
     6121 gatgatgcag agctaccgta atctgatccg ctttgcgcgt cgcaataacc ttagcgtctc
     6181 cgccagtccg caggatatcg gcgtactgac gcgtaaactg tatgccgcgt ttgaagcatt
     6241 gccaggtaaa gtgacgctgg taaacccgca gatttcaccc gatctctcgg aaccgaatct
     6301 gacctttatt tatgtgccgc cgggccgggc taaccgttca ggttggtatc tgtataaccg
     6361 cgcgccaaat attgagtcga tcatcagcca tcagccgctg gaatataacc gttacctgaa
     6421 taaactggtg gcgtgggcat ggtttaacgg cctgctgacc tcgcgcaccc gtttgtatat
     6481 taaaggtaac ggcattgtcg atttgcctaa gttgcaggag atggtcgccg acgtgtcgca
     6541 ccatttcccg ctgcgcttac ctgcaccgac accgaaggcg ctctacagcc cgtgtgagat
     6601 tcgccatctg gcgattatcg ttaacctgga atatgaccct acagctgcgt tccgcaatca
     6661 ggtggtgcat tttgatttcc gtaagctgga tgtcttcagc tttggcgaga atcaaaattg
     6721 cctggtaggt agcgttgacc tgctgtaccg caactcgtgg aacgaagtgc gtacgctgca
     6781 cttcaacggc gagcaatcga tgatcgaagc cctgaaaact attctcggca aaatgcatca
     6841 ggacgccgca ccgccagata gcgtggaagt cttctgttat agccagcatc tgcgcggctt
     6901 aatccgtact cgcgtgcagc aactggtttc tgagtgtatt gaactgcgcc tttccagcac
     6961 ccgccaggaa accgggcgtt tcaaggcgct gcgcgtttct ggtcaaacct ggggcttgtt
     7021 cttcgaacgc ctgaatgtat cggtacagaa gctggagaac gccatcgagt tttatggcgc
     7081 tatttcgcat aacaaactgc acggcttgtc agtgcaggtt gaaaccaatc acgtcaaatt
     7141 accggcggtg gtggacggct ttgccagcga agggatcatc cagttctttt tcgaagaaac
     7201 gcaagacgag aatagcttta atatctacat tctcgacgaa agcaaccggg ttgaggtgta
     7261 tcaccactgc gaaggcagca aagaggagct ggtacgtgac gtcagtcgct tctactcgtc
     7321 atcgcacgac cgcttcacct acggctcaag cttcatcaac ttcaacctgc cgcagttcta
     7381 tcagattgtg aaggttgatg gtcgcgaaca ggtgattccg ttccgaacaa aatcgattgg
     7441 caacctgccg cccgccaatc aggatcacga tacgccgcta ttacagcagt atttttcgtg
     7501 atgagcgtgc cggaaagcga ggcttatccg gcatgcaatc ttagcggaaa ctgactgttt
     7561 cacccgcctg ttgcgtcgcc gcctgttcca gcaaatccca gaaggtttcg ccgctgcgat
     7621 cgcaaatcca ttcatcgcct ttcaggtcaa aatggtagcc gccctgtttg gttgccagcc
     7681 acacctggtg cagcggctcc tggcggttaa taatgatttt gctgccgttt tcaaaggtaa
     7741 tggtcagtac gccgccgttg atctcgcagt cgatatcgct gtcgccatcc ctgtcgtcca
     7801 gacgctcttc aatggtcagc cacagttgat cagccaggcg atgaaattca ctgtcgttca
     7861 ttgttgtacc ctgtttttaa gtgatggcgg cagtatagcg gcatggggtc agggcttcaa
     7921 agtttgcacc tctgctgctg cgttccggca tgattcatcc gtcaccggaa taatgatgtc
     7981 tctgttttga acttcacgta cgttctcctc tctgacacga gttttcgccg ggcaggggga
     8041 gggcgtaatc cttacctctg tatcggtcca tgtataaaca ttgcgatcgt cataagcgaa
     8101 gcgatcgttt ttagttagcg gaccgttaac tggaataatt tgcaagctct ttgaccgtaa
     8161 ttggcggata aagtaagcat gctgtttgcc gctattaaat gagcgatcaa tgaccaagtc
     8221 tggcgtatcc agcgggatta tctcaagctg gtcttcacca aatgtcgata attttgttac
     8281 atacagccga tctttatatt gcgcaatatt gccagtaatc agatggaatt gttcaggttc
     8341 aatacctggt aggtctatcc acttgctcca ctgacggtcg ggcgtgagct gtttacgcca
     8401 gcgaacgcga ctttccccta cttcaaaaga gacgtcatca tctacgggta tcgctttgcc
     8461 tgataattga ccaaagggat aacgatgcag acccttattg tcgcgaaaaa ccagcagtga
     8521 atgaggtatc cagcgaatta tctcaaatga gtctgcattc gccgtaaggt gaacgccatt
     8581 taaataaata ttctgcccac tgcggataag cagatcattg gaatatgaat ttctgtttac
     8641 actcataaga cgtggattaa taccccacca tcttttttct aatacagtaa catcactgct
     8701 gctcccctga cgtcgaccgg acaagtagag atttttacta tccaccagcg tggtagagtt
     8761 accttcgata gcttttagtg tcgccagatc aacacgtgac gcgccgctat tactttcggt
     8821 gcgctgtccg tcaaaataga ggctatattt atcgaccgcg aagcggccca tagcctgaaa
     8881 gctggcaata tcgacagtgg gtgttcccgg tggattgcta acgattttgc cacgccagag
     8941 aataacgcgg ccgtctgtca cccagtggta ttcctcattt tctatgctta tttcatcgga
     9001 aggtatataa cccgttttcc cagagatatt tttcacaagg cgcaaaccgg ccctgctcat
     9061 ctccggaaac tgggcaatga ttttcttatc cccccaataa cctttaccgt aagacataac
     9121 accgtgatca ttgtaatagt cgaaccctat ggcctgtaca gcaggcgtca gaaatgtgtg
     9181 tcccaacagg atgagtgcaa ctgtgattat ttgacccttc attttgtcac ctcattgcgc
     9241 ggggcaggaa taaattcgcc gtcatcagta atttttccgt ccagatatcc cgttttgtaa
     9301 tttacgccat agacatatcc cgaatgttgt tccttccaga ttaagcgaac ttgtttcgga
     9361 tcagcggtgc ggtatcgcca gagctgcgcg ccatcaaagc tgtaaatgta gcggttatcg
     9421 cgggcatacc atctgcgcaa aaagggatgg tcattaagat gggcttcgcg ttcatccgga
     9481 gaacggttat ccagtaagat cagcggtccg tcagattcaa acacgtgtaa accggaatgg
     9541 tatggctacc cttcggcacg cgtaagtaaa tagccgtggc gtttcccggc gttgaaacgt
     9601 ttgttgataa taagattggg atcatcaagt ttcacgatct ccagcttacg gtcaccagac
     9661 tctgtttcga tgatggtata aagcgagtcc tgatactggg cgacagcatc actgatgggt
     9721 tgaaactgct ccggatcgag tccgggtaat tcttcctgtt tacaagcagg tcctttacgc
     9781 cagaacactt tcttttccag cagagaaaaa tccagacagt gctttgttaa atcttcatcc
     9841 cacgccagat tttccttgtc gaggacttta cgctgcagcc cgtttttatc acgccaggtc
     9901 aagagtgagc cgggcatcca gcgcacgacg gaaaaggtgt ccgggtcggc atcaagctgt
     9961 tcgccgttga gcaggatttt ggtccgtgta cgagccaggg tatcccaggg gccaaatggt
    10021 acggcaatgc aggggttaaa cgtggtggaa aactttcctc gctgatccca ggacttctgc
    10081 gccagcacac ggaaactctc ggggtcagcg aggcgatgcc cattgatata caagaaatta
    10141 gcgtcacgta atatcagccc cagtaagtca ggatcccagg gaggccggaa ttccacctga
    10201 tgcagcgttt tgatatccaa gctatttcca ccaccattgt catcggtgcg cttgccttca
    10261 aaatagagac tgtgtttatc tgcggcaaaa tcaccccatg ccttgaacga ggcgacatcg
    10321 acgggaggtg tgccgggagg gttttgcaca acttcacccg cgtagaggat attgcgacca
    10381 tcgctatgcc atgaataatg aggatactag gcctgtgcgc cctcatagag gtagtcggtt
    10441 atcaaatcag tcagaggcgg ttctttatcc cattcgtcta gtgttgtcgg gtctattaat
    10501 aaattgggta accgacgcaa actcttgtaa ttcacgtcag gtagagaaag atttgtttct
    10561 ttataagggc gcaggtacac cacgcctttt ccctgtttct ggtaaatatt atgttcaggt
    10621 gcggaaagcc gacatcccca tgccgacgat aaaaacaatg aacaagccaa caaacaaacc
    10681 acattgcgat agtgcataaa gccatcctgg cgcgaggtgt tgatcacgaa actgccagca
    10741 gaacataaat cctcacgagt aagcgttata ctcgcagcat ttcctcactt ttcagacttc
    10801 ataaagagtc gctaaacgct tgcttttacg ccttctcctg cgatgataga aagcagaaag
    10861 cgatgaactt tacaggcaat ccataatgaa aaacgtgttt aaggcactca ctgtattact
    10921 tactctcttc agcctgacgg gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga
    10981 taaaaacgca ccgccgccga ccaaaccggt agagacgcaa acgcaatcca cggtgccgga
    11041 taaaaacgat cgcgccactg gcgatggtcc atcccaggtg aattactaaa agtcagtttc
    11101 tgtacccgcg tgattggagt aaatgatgca gttctcgaaa atgcatggcc ttggcaacga
    11161 ttttatggtc gtcgacgcgg taacgcagaa tgtctttttt tcaccggagc tgattcgtcg
    11221 cctggcggat cggcacctgg gggtagggtt tgaccagctg ctggtggttg agccgccgta
    11281 tgatcctgaa ctggattttc actatcgcat tttcaatgct gatggcagtg aagtggcgca
    11341 gtgcggtaac ggtgcgcgct gctttgcccg ttttgtgcgt ctgaaaggac tgaccaataa
    11401 gcgtgatatc cgcgtcagca ccgccaacgg gcggatggtt ctgaccgtca ccgatgatga
    11461 tctggtccgc gtaaatatgg gcgaacccaa cttcgaacct tccgccgtgc cgtttcgcgc
    11521 taacaaagcg gaaaagacct atattatgcg cgccgccgag cagacaatct tatgcggcgt
    11581 ggtgtcgatg ggaaatccgc attgcgtgat tcaggtcgat gatgtcgata ccgcggcggt
    11641 agaaacgctt ggtcctgttc tggaaagtca cgagcgtttt ccggagcgcg ccaatatcgg
    11701 ttttatgcaa gtggttaagc gcgagcatat tcgtttacgc gtttatgagc gtggggcagg
    11761 agaaacccag gcctgcggca gcggcgcgtg tgcggcggtg gcggtaggga ttcagcaagg
    11821 tttgctggac gaagaagtac gcgtggaact ccccggcggt cgtcttgata tcgcctggaa
    11881 aggtccgggt cacccgttat atatgactgg cccggcggta catgtctacg acggatttat
    11941 tcatctatga agcaaccagg ggaagaactg caggaaacac tcacggagct tgatgaccgg
    12001 gcggttgtcg attatctgat taaaaatcct gagtttttta tccgcaatgc gcgcgcagta
    12061 gaagcgatac gtgtgccgca tccggtacgc ggcaccgttt cgttggtcga gtggcacatg
    12121 gcccgcgcac gtaatcacat tcatgttctg gaagagaaca tggcgttgtt gatggagcag
    12181 gctatcgcca acgaagggct gttttatcgc ctactctacc tgcagcgcag tctcacggcc
    12241 gccagcagtc tcgacgatat gctgatgcgc tttcaccgct gggcgcgcga tctcggcctg
    12301 gctggtgcga gtctgcgcct gtttccagat cgctggcgct taggtgcgcc gtcgaaccac
    12361 actcatctgg cattaagccg tcagtctttc gaaccgctgc gtattcagcg tttggggcag
    12421 gaacagcact atcttgggcc gcttaacgga ccggagctgc tggtggtgct accggaagcg
    12481 aaagcggtgg gatcggtggc gatgtcgatg ctgggaagcg atgctgattt gggtgttgtg
    12541 ctgtttacca gtcgcgatgc cagtcactat caacaagggc agggaacgca gttacttcat
    12601 gaaattgcgc tgatgttgcc ggagcttctg gagcgttgga ttgaacgcgt atgaccgatt
    12661 tacacaccga tgtagaacgc tacctacgtt atctgagcgt ggagcgtcag cttagcccga
    12721 taaccctact taactaccag cgtcagcttg aggcgatcat caattttgcc agcgaaaacg
    12781 gcctgcaaag ctggcaacaa tgcgatgcag cgatggtacg caattttgct gtacgcagtc
    12841 gccgtaaagg gctgggagca gcaagtctgg cgttacggct ttctgcgcta cgtagctttt
    12901 ttgactggct ggtcagccag aacgaactca aagctaaccc ggcgaaagga gtttcggcac
    12961 cgaaagcgcc gcgtcatctg ccgaaaaata tcgacgtcga cgatatgaat cggctgctgg
    13021 atattgatat caacgatccc ctcgctgtac gcgaccgtgc aatgctggaa gtgatgtacg
    13081 gcgcgggtct gcgtctttcc gagctggtgg ggctggatat caaacacctc gacctggagt
    13141 ccggcgaagt gtgggtgatg gggaaaggca gcaaagagcg ccgcctgccg attggtcgca
    13201 acgctgtggc gtggattgag cactggcttg atttgcgcga cctgtttggt agcgaagacg
    13261 acgcgctttt tctgtcgaaa ctgggcaagc gtatctccgc gcgtaatgtg cagaaacgct
    13321 ttgccgaatg gggcataaaa caagggctga ataatcacgt tcacccacat aaattacgtc
    13381 actcgttcgc tacgcatatg ctggagtcga gcggcgatct tcgtggtgtg caggagctac
    13441 tgggtcatgc caacctctcc accacgcaaa tctatactca tcttgatttt caacaccttg
    13501 cctcggtgta cgatgcggcg catccacgcg ccaaacgggg gaaataatgc gtttttaccg
    13561 gcctttgggg cgcatctcgg cgctcacctt tgacctggat gatacccttt acgataaccg
    13621 tccggtgatt ttgcgcaccg agcgagaggc gcttaccttt gtgcaaaatt atcatccggc
    13681 gctgcgcagc ttccagaatg aagatctgca acgcctgcgc caggcggtac gggaagcgga
    13741 acccgagatt tatcacgacg tgacgcgttg gcgttttcgt tcgattgaac aagcgatgct
    13801 cgacgccggg ctgagtgccg aagaagccag tgcaggcgca cacgcagcaa tgattaactt
    13861 tgccaaatgg cgcagccgaa tcgacgtccc gcagcaaact cacgacacct taaaacagct
    13921 ggcgaagaaa tggccgctgg tggcgatcac caacggtaac gcccagccgg aactgtttgg
    13981 tttgggggat tattttgagt ttgtgctgcg cgctggcccg cacgggcgct caaaaccgtt
    14041 cagcgatatg tactttttgg ctgcggaaaa actcaacgtg ccgatcggcg agatcttaca
    14101 tgttggggac gatctcacca ctgacgtggg tggggcaatt cgcagcggaa tgcaggcttg
    14161 ttggatcaga ccggaaaatg gcgatctgat gcaaacctgg gacagccgtt tactgccaca
    14221 tctggaaatt tcccggttgg catctctgac ctcgctgata taatcagcaa atctgtatat
    14281 atacccagct ttttggcgga gggcgttgcg cttctccgcc caacctattt ttacgcggcg
    14341 gtgccaatgg acgtttctta cctgctcgac agccttaatg acaaacaacg cgaagcggtg
    14401 gccgcgccac gcagcaacct tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta
    14461 ctggtgcatc gtatcgcctg gctgatgagc gtggaaaact gctcgccata ctcgattatg
    14521 gctgtgacgt ttaccaacaa agcggcggcg gagatgcgtc atcgtatcgg acaactgatg
    14581 ggcaccagcc agggcggcat gtgggtaggc accttccacg ggctggcgca ccgcctgctg
    14641 cgtgcgcacc atatggacgc caatctgccg caggatttcc agatcctcga tagcgaagac
    14701 cagctacgcc tgcttaagcg tctgatcaaa gccatgaacc tcgacgagaa gcagtggccg
    14761 ccgcgccagg caatgtggta catcaacagc cagaaagatg aaggcctgcg tccgcatcat
    14821 attcaaagct acggtaatcc ggtggagcag acctggcaga aggtgtatca ggcgtatcag
    14881 gaagcgtgcg atcgcgcggg cctggtggac ttcgccgagc tgctgctgcg cgctcacgaa
    14941 ctgtggctta acaagccgca tatcctacaa cactaccgcg aacgttttac caatatcctg
    15001 gtggacgaat tccaggatac caacaacatt cagtacgcgt ggatccgcct gctggcgggc
    15061 gacaccggca aagtgatgat cgtcggtgat gacgaccagt caatctacgg ctggcgcggg
    15121 gcgcaggtgg agaatattca gcgtttcctt aatgatttcc ccggtgccga aattattcgt
    15181 ctggagcaaa actaccgctc taccagcaat attctgagcg ccgctaacgc cctgattgaa
    15241 aacaataacg ggcgtctggg taaaaaactg tggaccgatg gcgcggacgg tgagcctatt
    15301 tccctctatt gcgcttttaa cgaactcgat gaagcgcgtt ttgtggttaa ccgcatcaaa
    15361 acctggcagg acaacggcgg ggcgcttgcc gagtgcgcca ttctctaccg cagtaacgcc
    15421 cagtcgcgtg tgctggaaga agcactgttg caggcgagta tgccgtaccg tatttacggc
    15481 gggatgcgct tcttcgaacg ccaggaaatc aaagatgcgc tctcgtatct gcgcctgatt
    15541 gccaaccgta acgacgacgc ggcctttgag cgtgtggtga atacgccaac gcggggtatt
    15601 ggtgaccgga cgctggacgt ggtacgtcag acatcgcgcg atcgccagtt aacactctgg
    15661 caggcatgta gtgaactgtt gcaggaaaaa gccctcgccg gacgtgctgc cagcgcctta
    15721 cagcggttta tggaactgat cgacgcctta gcgcaggaaa ctgccgatat gccgctgcat
    15781 gtacagactg accgggtaat taaagactcc ggcctgcgta ccatgtacga gcaggagaag
    15841 ggcgaaaaag gtcagacgcg tatcgaaaac ttagaggaac tggtgacggc aacgcggcag
    15901 ttcagctaca acgaagaaga cgaagattta atgccgctgc aggcgttcct atcccatgcg
    15961 gcgctggaag caggcgaagg gcaggcggat acctggcagg atgcggtaca actgatgacc
    16021 atgcactcgg cgaaaggtct ggagttcccg caggtgttta tcgtcggtat ggaagagggc
    16081 atgttcccaa gccagatgtc gctggatgaa ggcgggcgtc tggaagaaga acgccgtctg
    16141 gcctacgttg gcgtaacccg cgcgatgcag aaactgacgc tgacctacgc ggaaactcgc
    16201 cgtctgtatg gcaaagaggt ttaccatcgc ccgtcgcgct ttattggcga gttgccggaa
    16261 gagtgtgtgg aagaggtgcg cctgcgcgcc acggtaagcc gcccggtcag ccatcagcgg
    16321 atgggtacgc cgatggtcga gaacgacagc ggctacaagc tcggccagcg tgtacgccac
    16381 gctaagtttg gtgaaggcac catcgtcaat atggaaggca gcggtgaaca tagccgtttg
    16441 caggtggcat tccagggcca gggaatcaaa tggctggtgg cagcttacgc ccggctggag
    16501 tcggtgtaac gttgccggat gcggtgctgc gcaccttatt tggcctaaaa aatcattcag
    16561 attcaataaa ttgcaacgtc atgtaggcag gataaggcgt ttacgccgca tccggcatca
    16621 gcagcatatt tagtatctga cacaaagcta tcattctaac ctttccgctc gacggaaatc
    16681 atggtgacaa aaggatagcg ttgccacggg attgccccgc ctttcatata catatgtgaa
    16741 atcgtgccac caagataaag cagctgctca acgttcagtt tcgctttggc ataacaggca
    16801 aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcattccc atgtttatta
    16861 atcccaacac cgttacgaat tttacgtgag gcgacgttgg gatgaatacg cggattaatt
    16921 acaccgtttt ccatcaacat tggtcctgac tgcaccgcaa actgaatatc tttactggct
    16981 ttaaaagcat ccagacgaac gatgccgact ttatctcccg cgacataaaa cacgccgcca
    17041 ggacggataa agaaattccc ttcacctgaa gcgagattta acgctacctt c
//
LOCUS       DADWTH010000085        16902 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.086, whole genome shotgun sequence.
ACCESSION   DADWTH010000085 DADWTH010000000
VERSION     DADWTH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.086"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..882)
                     /locus_tag="JNY02_002935"
     CDS             complement(<1..882)
                     /locus_tag="JNY02_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBB9434398.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="JNY02_002936"
     CDS             989..1285
                     /locus_tag="JNY02_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9434399.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNY02_002937"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNY02_002937"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBB9434400.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNY02_002938"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNY02_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBB9434401.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNY02_002939"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNY02_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBB9434402.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4297..4380
                     /locus_tag="JNY02_002940"
     CDS             4297..4380
                     /locus_tag="JNY02_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBB9434403.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNY02_002941"
     CDS             complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNY02_002941"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBB9434404.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5858..6700)
                     /gene="rlmJ"
                     /locus_tag="JNY02_002942"
     CDS             complement(5858..6700)
                     /gene="rlmJ"
                     /locus_tag="JNY02_002942"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBB9434405.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6903..8945
                     /gene="prlC"
                     /locus_tag="JNY02_002943"
     CDS             6903..8945
                     /gene="prlC"
                     /locus_tag="JNY02_002943"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBB9434406.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNY02_002944"
     CDS             8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNY02_002944"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBB9434407.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNY02_002945"
                     /pseudo
     CDS             complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNY02_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNY02_002946"
     CDS             complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNY02_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBB9434408.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12354..12689
                     /gene="uspB"
                     /locus_tag="JNY02_002947"
     CDS             12354..12689
                     /gene="uspB"
                     /locus_tag="JNY02_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBB9434409.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNY02_002948"
     CDS             complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNY02_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBB9434410.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14491..15690
                     /locus_tag="JNY02_002949"
     CDS             14491..15690
                     /locus_tag="JNY02_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB9434411.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4300 a         4219 c         4364 g         4019 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattggata
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
     3721 gcgtcggttt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     3781 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     3841 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     3901 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     3961 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     4021 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     4081 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     4141 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4201 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     4261 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4321 cttggggcgt taattgtgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4381 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4441 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4501 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     4561 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     4621 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     4681 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     4741 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     4801 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     4861 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4921 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     4981 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5041 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5101 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     5161 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5221 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5281 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     5341 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     5401 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5461 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     5521 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     5581 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     5641 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     5701 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     5761 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     5821 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     5881 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     5941 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6001 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     6061 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     6121 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6181 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     6241 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     6301 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6361 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     6421 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6481 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     6541 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     6601 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     6661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     6721 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     6781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     6841 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6961 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     7021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7081 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7141 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7201 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     7261 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     7321 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     7381 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7441 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     7501 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     7561 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     7621 gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     7681 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     7741 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     7801 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     7861 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     7921 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     7981 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8041 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8101 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8161 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     8221 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8281 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8341 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8401 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     8461 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     8521 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     8581 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     8641 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     8701 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     8761 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     8821 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     8881 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8941 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9001 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9061 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     9121 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9181 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9241 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     9301 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     9361 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     9421 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     9481 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     9541 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     9601 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     9661 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     9721 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     9781 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     9841 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9901 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9961 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10021 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
    10081 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagtcgt
    10141 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10201 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10261 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10321 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10381 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10441 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10501 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    10561 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    10621 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    10681 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    10741 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    10801 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    10861 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10921 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
    10981 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11041 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11101 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11161 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    11221 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11281 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11341 attacaacag aaaataacca gctgatcaca ctaatgatgg tgttaaattt tgtattggca
    11401 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    11461 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    11521 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    11581 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    11641 accaccagat ccatatcgta tttcttgatt gcatcgacca gaacctgacc caggtcgccg
    11701 ctgccgctca gggtttcagt gattggatag cctgcattag tggaaagctc ggtcagcgca
    11761 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    11821 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    11881 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    11941 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    12001 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    12061 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    12121 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    12181 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    12241 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    12301 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    12361 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    12421 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    12481 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    12541 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    12601 gtgagcgggt gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca
    12661 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    12721 cgggccagta aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagaa
    12781 ccagtacagc ccgccggaaa gtagtaccgc agccggaagg gtaaacaccc aagccatcag
    12841 aatactggtt acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    12901 agaagaggag agtacgtgag tggtggaaac cggcatcccg gtataactcg ccaggccgat
    12961 tgacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc
    13021 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    13081 cgccagcgcg accgccatga tgatccacac cggcgcatac tcgatggtgc taagcatgtc
    13141 ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acaccaggca tcttcaccac
    13201 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctgactac gttgatcaag
    13261 cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc
    13321 gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg
    13381 agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa
    13441 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    13501 catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat
    13561 gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag
    13621 cgcgatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    13681 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    13741 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    13801 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    13861 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    13921 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    13981 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    14041 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    14101 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    14161 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    14221 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    14281 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    14341 ttttttgatt tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag
    14401 gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt
    14461 cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc
    14521 gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg
    14581 ctgatcgata atggtaaaat actagggcgc aaaatcctta tgtctggcgg tgggcgctgc
    14641 aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt
    14701 aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa taaacacggt
    14761 atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt
    14821 gtcgacatgc tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa
    14881 gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg catgactgtc
    14941 ggttgcgaaa agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcatcg
    15001 ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt
    15061 ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg
    15121 gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc
    15181 acccaccgcg gcttatctgg accggcggtg ttgcagattt caagctactg gcaaccgggt
    15241 gaatttgtca gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag
    15301 cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct accgaagcgg
    15361 ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac
    15421 gtgtgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc
    15481 actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactttct
    15541 tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac
    15601 gttaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt
    15661 gcgcaggatt tggcgtcaat tgtgaaataa ccatgacgtt ttcccgtcag ccgtttgtat
    15721 atgtcgttat taagtcattt tggtacggtt ctgcatccga cattctggtg ttaactcgct
    15781 gttttgttag cgaattaacg ctattgccgt tgtggttatt ttatagtgta tttattctca
    15841 gagaatatat attactgaaa taaaaaacac aaagaataac caaaggaaat tatttgaaac
    15901 gtaaaaggta tatattttta atctgtaaaa tttaaaatca ccccctctgg attgaatcaa
    15961 cttgctctat ttaagacgat tgtttttaca ttttcaccac ctgtatcagg tggtgttttt
    16021 ttattggcgg caatgccttt atcttatttt tattacgttg taaatatggc tattcctaaa
    16081 tgaaatttga tttgctcagt ttatttgtga tgtcagggga gtgcttgcca tgagagtcgt
    16141 gatattatta ttcatcattt ttataccgag tgtggcggat gctttttcat tcgatagttc
    16201 aataattaat ctcaaatgtc ctaggcgtgg aactatcgaa ataatattac acagatacga
    16261 acacacccag gaaagctggg gaggggagaa ttttgaaacc gggagtgggt atagtcatga
    16321 gggcgcgtta tttatttttg actttgccaa tcaggataaa atgatttaca atcgcgcgga
    16381 gaattcgttt gctttttggt atgaatcttc ccggaaatta gttaactgta acttaataac
    16441 gcttaaaagc acatatcctg tcaaccttcc ttatgttcat ggctgatgtg atgtgatgtg
    16501 atgtgtgtcg ggaactaaca tcttcatctt tgtatatgac attgcgataa tgttaagctg
    16561 gtctttgtca taaatttacc cttagagata ctactccctt ctgtcaatat tattcaagcc
    16621 gaacgttata ctggcttttg tcggtcacgg atatcttttt ttcagtgaga attcaaagtg
    16681 agggtatata agaagtcgta tgagtaagta tctttgttgt ggtgggttat tattttatgg
    16741 gcaaaatatt aagcagcgaa atcttttttt tgcccagacg ttttttgtag aaaaaagaat
    16801 ttattagtcc ggctgaaaag caagaactgc tataactaat taagcgtaac ggaacggtac
    16861 aaaacatgcc cttacaacaa ttttcccgat tagcgctcac tc
//
LOCUS       DADWTH010000086        16758 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.087, whole genome shotgun sequence.
ACCESSION   DADWTH010000086 DADWTH010000000
VERSION     DADWTH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.087"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            191..2045
                     /gene="ldtD"
                     /locus_tag="JNY02_002950"
                     /pseudo
     CDS             191..2045
                     /gene="ldtD"
                     /locus_tag="JNY02_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            2226..2774
                     /locus_tag="JNY02_002951"
     CDS             2226..2774
                     /locus_tag="JNY02_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBB9434412.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2801..3448
                     /gene="gloC"
                     /locus_tag="JNY02_002952"
     CDS             2801..3448
                     /gene="gloC"
                     /locus_tag="JNY02_002952"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBB9434413.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3671..4861)
                     /gene="aspC"
                     /locus_tag="JNY02_002953"
     CDS             complement(3671..4861)
                     /gene="aspC"
                     /locus_tag="JNY02_002953"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBB9434414.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5046..6134)
                     /gene="ompF"
                     /locus_tag="JNY02_002954"
     CDS             complement(5046..6134)
                     /gene="ompF"
                     /locus_tag="JNY02_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBB9434415.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6736..8136)
                     /gene="asnS"
                     /locus_tag="JNY02_002955"
     CDS             complement(6736..8136)
                     /gene="asnS"
                     /locus_tag="JNY02_002955"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBB9434416.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8305..9507)
                     /gene="pncB"
                     /locus_tag="JNY02_002956"
     CDS             complement(8305..9507)
                     /gene="pncB"
                     /locus_tag="JNY02_002956"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBB9434417.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9773..12385
                     /gene="pepN"
                     /locus_tag="JNY02_002957"
     CDS             9773..12385
                     /gene="pepN"
                     /locus_tag="JNY02_002957"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBB9434418.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12592..13359)
                     /gene="ssuB"
                     /locus_tag="JNY02_002958"
     CDS             complement(12592..13359)
                     /gene="ssuB"
                     /locus_tag="JNY02_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBB9434419.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTVGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLFDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13356..14003)
                     /locus_tag="JNY02_002959"
                     /pseudo
     CDS             complement(13356..14003)
                     /locus_tag="JNY02_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(14015..15160)
                     /gene="ssuD"
                     /locus_tag="JNY02_002960"
     CDS             complement(14015..15160)
                     /gene="ssuD"
                     /locus_tag="JNY02_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBB9434420.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15157..16116)
                     /gene="ssuA"
                     /locus_tag="JNY02_002961"
     CDS             complement(15157..16116)
                     /gene="ssuA"
                     /locus_tag="JNY02_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBB9434421.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16109..16684)
                     /gene="ssuE"
                     /locus_tag="JNY02_002962"
     CDS             complement(16109..16684)
                     /gene="ssuE"
                     /locus_tag="JNY02_002962"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBB9434422.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4186 a         4319 c         4289 g         3964 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga tgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cacaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg caggatggaa tgacaaacgt atttctgatg
     1861 ctctgaagca gggtgataca cgttacgtca atattcggca gtcgattccg gtgaatctct
     1921 actacctgac ggcctttgtt ggtgcagatg atcgtaccca gtatcgtaca gatatttaca
     1981 attatgatct gcctgcgcga tccagctcgc aaatcgtatc gaaagcggaa caattaatca
     2041 ggtaaatgaa gtagttcggg gaaatcaatt gtcgtaataa ttagcatgat tgggggcgat
     2101 tctctgcagc ccccgtcact gctggggttg agtcatcttg acgtctgctt tacgggcggt
     2161 taaggtgcct cttgtgcgcc agaagtgcat ataaacgata acattgacct gtagacttga
     2221 ttatcatgga caaattcgac gctaatcgcc gcaaattgct ggcgcttggt ggcgttgcac
     2281 tcggtgccgc catcctgccg acccctgcgt ttgcaacact ctctacccca cgcccgcgca
     2341 ttttgacact caataatctt cataccggag agtcaatcaa agcggagttt ttcgatggca
     2401 gaggctatat tcaggaagaa ttggcaaaac ttaaccattt tttccgcgat taccgcgcga
     2461 acaaaataaa gtccatcgac ccaggattat tcgaccagtt gtatcgcctg caagggttgt
     2521 taggcacgcg caaaccggtg caactcattt ccggttatcg ttccattgat accaacaatg
     2581 aactacgcgc ccgcagccgt ggagtagcga agaaaagcta tcacactaaa ggccaggcga
     2641 tggatttcca tattgaaggt atcgcgttaa gcaatattcg caaagccgcg ttatctatgc
     2701 gcgcaggtgg tgtaggatat tacccacgta gtaactttgt gcatattgat accgggccag
     2761 cacggcactg gtagtaatcg cttaacgaaa caggggcagt atgaactatc gtattattcc
     2821 ggtcaccgca ttctcccaga actgttcatt gatctggtgt gaacaaaccc gcctggccgc
     2881 actggtcgat cctggcggcg atgcggaaaa aatcaaacag gaagttgatg acagcggcct
     2941 gacactgatg cagatcctgc tgacgcatgg tcatctggac cacgttggcg cagcggcgga
     3001 actggcgcaa cattacggcg tgccggtttt tggcccggaa aaagaagatg agttctggct
     3061 gcaaggcttg cctgcgcaaa gtcgtatgtt tggtctggaa gagtgccagt cgctgacgcc
     3121 agatcgttgg ctgaacgaag gcgataccat cagcataggg aatgtgactt tacaggtgtt
     3181 acattgccct gggcatacgc cgggtcatgt cgtgtttttt gatgatcggg caaagctgct
     3241 gatttctggc gatgttattt tcaaaggcgg agtagggcgc agtgacttcc cgcgtggcga
     3301 tcataatcaa ctgatttctt caatcaaaga taaattgctg cctctggggg atgacgtgac
     3361 atttattccg ggtcacggac cattatctac acttggttat gagcgcctgc acaatccctt
     3421 cctgcaagac gaaatgcccg tctggtaaag gcgcataaaa aagtccactt ttaatgcggg
     3481 cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg
     3541 gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag
     3601 cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg
     3661 tttttaacgc ttacagcact gccacaatcg attcgcacag cggagccatg ttatctggtg
     3721 tcatcccggc cacatttacg cgaccagaag caacagcata tacgccaaac tcttcgcgca
     3781 gacgcagcac ttgttctttt gtcagaccac tgaaggagaa catgccgttc tgtttgatga
     3841 taaagctgaa gtcgcggttt gcaccttttt cctgcagcgt attgacgaac aactgacgca
     3901 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaatgcat
     3961 cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggagggtta gagtagttag
     4021 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac gatttcactg tcggcagcaa
     4081 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
     4141 caacaatcag ctctttatgc atagccgaga aagcgcgcag tccttcagca tcttcttcca
     4201 gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg
     4261 agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
     4321 agcagccatg gaacagcact acgtcgccag cctgagcttc gttcagactg ttaatcagtg
     4381 catcgaagtc aagggtgtga ttttccgcat cataataagc gtattcacga acttccagac
     4441 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacacacgct
     4501 taacgctggt attttttgcc aggaagtcgg cagccacgcg tagtgcgcca gtgccacccg
     4561 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
     4621 gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg
     4681 tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
     4741 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
     4801 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
     4861 tgacgaggtt ccattatggt tatagaaggg aagtccgcta tcagggtaac gggagattta
     4921 caaaattcca attattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
     4981 tggaaggcat aaaaaaaaca ggaccaacgt cctgtttttt cggcatttaa caaagaggtg
     5041 tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt
     5101 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
     5161 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
     5221 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
     5281 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat
     5341 ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
     5401 ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt
     5461 tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct
     5521 tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca
     5581 cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag
     5641 ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat
     5701 gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca
     5761 ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc
     5821 agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta
     5881 tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca
     5941 agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa
     6001 aaataatgca gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag
     6061 atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata
     6121 ttgcgcttca tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt
     6181 caataagttc tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt
     6241 gtctgcaggc atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat
     6301 aaagatttag tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggagctttgt
     6361 gaacaccgtc atatttccat agaaatgtga tgatatttac agcaattttt atctatttat
     6421 atgatttact tatatttaaa ttaactaaac ggaaattttg tttctgatgg aaactttatc
     6481 gacctggcac aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt
     6541 gctatcagct aagaaaaaac gaatccgcaa taagaatgcg ggttattttt tgtgcgtaaa
     6601 tgttgcaatg taatattccg taacaggatg atcgttatgg cgggggtgca aatagattgc
     6661 ttgacttaac aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct
     6721 ctttgaggaa gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc
     6781 acgcacgttt tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc
     6841 tgaatgcgga acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt
     6901 caggcccatt tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc
     6961 accaatgatc tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc
     7021 ttcgttgagg cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg
     7081 tgctttaaag tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca
     7141 gtaaaccggg ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta
     7201 atccacctgt gcgaaatcgg cttcaatgaa gcgttccaga cgtgaaacgg catctttatc
     7261 tacgcgttca gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac
     7321 atatttcagc atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc
     7381 cggctccagc atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa
     7441 agtcgggccg aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg
     7501 gccagatacg gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc
     7561 ctgatcgtta cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc
     7621 accttcggta tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt
     7681 aaagaagcga tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag
     7741 gtttgtgcgc ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc
     7801 cgccatcggg taagtgtctg gatcttcaac ccaaccagca acttcaacct tgctggcctg
     7861 aatttcaaat tgttgcccct ggcccggcga tgccacgact ttacccgtca caatgaccga
     7921 gcagccggtg gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac
     7981 agcctgtaca ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt
     8041 tgaatctcgg cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg
     8101 gccctggagt acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg
     8161 aaaaaataaa cacttgtcca cccgtaaatg ggggggatac ctatgttacc tggcatctgc
     8221 aatcagacaa gcagaattcg caaatgcagc gaaagatttc ggaattaaag gtaaaaaagg
     8281 gagccgatta gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc
     8341 tttgcgcagc gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga
     8401 aagtttcgcc accggtttac cgttacactc taccaactta atcacaatat tcaggggttt
     8461 tacctggggg atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga
     8521 agagaagtgg cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag
     8581 cgttttactc tgtggatcaa ttcccagctt ttcataatgt gcaatggctt tttcacccca
     8641 ttcaaccggg tcgccagagt catgacgcag gccctgatac cgactagcga actcgacacc
     8701 gaaatcacgc aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc
     8761 gggatactct tccagccagg cagcaagtgc agctcgctgg ctgttggcta gatccgggct
     8821 gatttgctga tgtgcctgga accattcgtg tgcctgtgtt cccatcggcg tgagggaaag
     8881 ccgacgcgcc agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt
     8941 aacgatggtt tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg
     9001 gaagcgcgac atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag
     9061 cgtgtcgagg gcttgtgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact
     9121 gataaccgcc agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg
     9181 aatatccagc ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaactc
     9241 gcgtaaccag ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc
     9301 atcatcctgc aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata
     9361 aatacccagc agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata
     9421 gtgatgaaac acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg
     9481 cagaacagga gaagcgaatt gtgtcatagg tgcgcagtag cgtcctcgta caggagcgtt
     9541 tagtacaata aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac
     9601 cccgatcaca ccatatgcca cctttctgga cgatggcatt acgcggtgta tgttataaaa
     9661 atgtagcaat aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg
     9721 caacaggaat agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca
     9781 acagccacaa gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat
     9841 tgacttgacc tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt
     9901 ccgtcatggt gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc
     9961 tgttcatatt aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg cactggtcat
    10021 cagtaatttg ccggagcgtt ttacgcttaa gatcattaat gaaataagcc cggcggcgaa
    10081 tacggcgctg gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga
    10141 aggtttccgc catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac
    10201 caaaattatt gccgataaaa tcaaatatcc cttcctgctt tccaatggta accgcgttgc
    10261 gcaaggcgaa ctcgaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc
    10321 gtgctacctg tttgcgctgg tggcaggcga ctttgatgta ctgcgcgata cctttaccac
    10381 gcgttctggt cgcgaagtag cactggagct gtacgtcgat cgcggcaacc ttgatcgcgc
    10441 gccgtgggcg atgacctcgc tgaaaaactc catgaaatgg gatgaagaac gctttggcct
    10501 ggagtatgac ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat
    10561 ggagaataag ggtctgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc
    10621 caccgacaaa gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg
    10681 gaccggtaac cgagtgacct gccgcgactg gttccagctc agcctgaaag aaggtttaac
    10741 cgtcttccgc gatcaggaat tcagctctga ccttggttcc cgcgcagtta accgcatcaa
    10801 taatgtgcgc accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc
    10861 aatccgcccg gacatggtca ttgagatgaa caacttctac accctgaccg tttacgagaa
    10921 gggcgcggaa gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg
    10981 gatgcaactc tatttcgagc gtcatgatgg tagcgcagcg acctgtgacg actttgtgca
    11041 ggcgatggaa gatgcgtcga atgttgatct ctcccatttc cgccgttggt acagccagtc
    11101 cggtacgccg gttgtgaccg tcaaagacga ctacaatccg gaaactgagc agtacaccct
    11161 gaccatcagc cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat
    11221 tccgtttgcc atcgaattgt atgacaacga aggcaaagtg atcccgttgc agaaaggcgg
    11281 tcatccggtg aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa
    11341 tgtctacttc cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga
    11401 atataagtgg agcgatcagc aactgacctt cctgatgcgt catgcgcgta atgatttctc
    11461 ccgttgggat gcggcgcaaa gtttgctggc aacctacatc aagctgaacg tcgcccgtca
    11521 tcagcaaggg cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcggtgct
    11581 gctcgatgag aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa
    11641 tgaaatggct gaactgttcg atatcataga cccgattgct attgccgaag tacgcgaagc
    11701 actcactcgt actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa
    11761 ttaccagagc gagtaccgtg ttgagcatga agatattgca aaacgcactc tgcgtaatgc
    11821 ctgcctgcgc ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca
    11881 ataccatgaa gcaaacaata tgaccgatgc gctggcagcg ctttctgcgg cggttgctgc
    11941 acagctgcct tgccgtgacg cgctgatgca ggagtacgac gataagtggc atcaggacgg
    12001 tctggtgatg gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga
    12061 gacggtgcgt ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg
    12121 ttcgttgatt ggtgcgtttg cgggcagcaa cccggcagcg ttccatgccg aagatggcag
    12181 cggttaccag ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtagc
    12241 ttcacgtctg attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa
    12301 aatgcgcgcg gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctttacga
    12361 gaagataact aaagcactgg cttgatactt ttgagccaaa atgcctgatg cgacgcttga
    12421 cgcgtcttat caggcctaca actccgttga aattgctggt tttgtaggct ggataaggcg
    12481 ttcacgccgc atccggcagc cgtgcccaac tgcctgatgc gatgcttgac gtgtcttatc
    12541 aggcctacaa gtccgttgaa aatgctggtt ttgtgcgcca catccggcat attaaccatg
    12601 acgccgaatc agttgttcag aatgcccacg ccgcattacc cgttgcaata cttccgcttc
    12661 cagctccgct aatcgcaccg accctaatcg acgcggacgg gggatatcca ctgtcaaatc
    12721 cagactaatt tttccctctt caattaacaa cacccggtca gccattgcta ccgcttcgct
    12781 cacatcatgc gtcaccaaca gtacggtaaa gccgtgctgc tgccaaagtg acacaatcaa
    12841 atcctgcatc tcgagtcgcg ttaaggcgtc cagtgccccg agcggttcat caaacaacaa
    12901 taaacccggt cgatgaatca acgctcttgc cagcgccaca cgctgtttct gcccgccaga
    12961 aagtgcggca ggccattccc cggcgcgatt ctccagtcct accgcagcca acgcccgacg
    13021 cgcggcatcg cgccactgac ctttaaggcc taacccaacg ttatcaatca ccgatttcca
    13081 cggcagcaga cgcgcatcct gaaacatcat tcgcgtatca tcctgaattt cagccagcgg
    13141 tgtggtgccc gctaacacat cgcctacggt tggcgtttcc agaccggcca gcaggcgcag
    13201 cagggtactt ttaccaccac cgctgcggcc caccaccgcc acaaactgac ctgccggaat
    13261 atgtaaatcc agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag
    13321 caacaatggt gtgccctggt tcagacgagc agtattcata tcgtggcctc cttcaaatga
    13381 taagccgggt tccagctcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt
    13441 ttgccgagca gggcgtaaag aataatagcg actaccacca cgtctgtttg caaaaactct
    13501 cgcgcattca tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc
    13561 agcgtcagcc acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca
    13621 tcgggcagga tcacatggat aaacagcggt atgccggata atccatagct acgcgccatc
    13681 tccaccagcc cgcgatcgat attacggatg ccatgccagg tgttgatata aatggggaac
    13741 agcgtaccga gcgccaccag aaagattttc gcggattcat caatgccaaa ccacaaaatc
    13801 accagcggga tcagcgccag atgctgccac agttcgccgc tggcggagag cgtccagaac
    13861 gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg acagccagcc aaccgaggag
    13921 gccagttgcc acaccgccac gatgcccacc ggtaaaaacc agggggcaac gcgcaataac
    13981 tacttcttca ctggcgttgc catcatcgtg ttccttagct ttgcgcgact ttacggggga
    14041 taaaatcatt cgccaccgct tcgccttgcg gattcagcgg ctgcggctgg ggaatttccg
    14101 gaatggcgac atccagatgt gggaacagca actcgccaac ccgatacgct tcttccagat
    14161 gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc ggcgtattcg ttgattcgcg
    14221 cggcgaccgt aggaccatcg cccaccagcg ccgttccggc accgccgcgc actaagccaa
    14281 cgcccgccca taaattgggg ctgatctcca gattgtcgcg cttgccgtta tgtagcgccg
    14341 ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc ggcttgtgct ttggcgatag
    14401 tctcatcatc aagatgcgag attaaccgct cggcggcctg ccacgcttcg tcgttagttt
    14461 cacgaacaat cacatgcaga cgaataccga aacgaatttt gcgcccatac gcggcagctt
    14521 tcgcccgcac ttgttcgatt ttctctttaa caagttccgg cggttcgccc caggtgaggt
    14581 agagatcaac ttgttctgcc gccagctcct gggcgacatc tgacgatccg ccaaagtaaa
    14641 gcggaggata aggctgttga atcggcggga agagcagttt tgctccgcgc acatgaatat
    14701 gtttaccgtt gaaattgacg gtttccccca gcaataagcg ccgccagacc tgggtaaatt
    14761 ccgccgaggc ttcgtagtgc tcgctatgat caaggaacac gccgtcgcct gccagctctt
    14821 gtggatcgct gcctgtgacc agattaaaca acgcacgacc atttgagaga cggtcaagcg
    14881 tggcggcctg gcgggcggca acagtaggtg aggttacgct gggacgcagg gcgacaagaa
    14941 acttcagccg ctgcgtcacc gggatcatcg acgctgcaac cagccacgca tcttcacacg
    15001 atcgccccgt tgggatcagc acaccggtat agcccagacg atccgccgct tgcgcaattt
    15061 gttgcagata accgtgatca accgggcgtg aaccttcttc cgttcccaga tagtgcccgt
    15121 caccgtgggt cggtaaaaac cagaacatat tcagactcat aattgttttc cttccagttg
    15181 agtgggctgc cagatgcgct ggcgaatatc gacttttttc ggcaccagac ggttttcata
    15241 aaacagatct gccgtttgct gctgtaaggc ggcaacctcg gcgttaaccg gtttgatggt
    15301 ggtaggaggg cgatgatcta agtaagaggc aatcaccggt gccggtaagc ccatcgtttt
    15361 tgccagtaaa gcgatgcttt gttcgcgctg gctgcgggtt aacgcatcgg cctcactaaa
    15421 ggttgccagt acgccctgaa taaaagcgcc gtttttttct gcatagggac gagctgccag
    15481 ataaaacgat ccagtttgat tgagatcggt gccgtctttc agcacccgca cgccgccctg
    15541 taataacgca gcggagtagt agggatccca gatagcccag gcgtcaacgt taccttgctg
    15601 gaacgcggcg cgggcaccgg caggcgtcag gtaagtgggc tggatatcgg tgaatttaag
    15661 tccggcctga cgcagtgcac gcagtaaaag gttgtgtgaa ctggaacctt tctgaaaggc
    15721 aactttgtga cctttaagat cggctacggt tttgatcggg ctgttttctg ccaccagaat
    15781 cacttcggct ttgggcttcg gtggctcgac gcccacatat accaaatcag ccccggcagc
    15841 ctgggcaaag attggcggaa tatccccggt actgccgaga tcaatactgc caacgtttaa
    15901 cgcttccaac atttgcggac ccgcggggaa ctccacccag gagatttttg attgcggata
    15961 gcgtttttcc agtaactggt ggctttttgc cagcaccata ccaatactgc ctttctgata
    16021 gcctatacgt aacgcttcag gcgaggattc tgcagcaacc gcaaaagtag agacgctaag
    16081 caatcccgcc agcgccagtt taatgatgtt acgcatgggc attacctcgc agagacagaa
    16141 ggtcaggaac ctgaacatcg cggcggtgca atgcctgcca gaaagtttct agcgcggtat
    16201 caagatgggg ttgcagattt ggcgtgaact ggggtctgtg atggtaatca attaattgtg
    16261 agtcatcggc aaacacgccg tgcaggatct cctgagcttt cagtgcgctt aaaactggtt
    16321 taagggcata atcgaccgcc agcagatggg ccacggtacc gcccgtcgcc agcggtagca
    16381 ccactttgcc ttgcaaagcg cgctctggca gcaggtcgag cagggttttc agcgcgccgg
    16441 aataggcggc tttatacaca ggcgtggcga caatcagccc atcagcctgt tgcagctgtt
    16501 cggtgaaggt cttgagtgcc ggactatcga aacgagcata aagcagatct tccggggcga
    16561 agttttgcag attccagtga taaacctcta catctaggcc atttagtttt tcccgcgcat
    16621 attccagcaa ggagctggag cgagaaggaa agcgaggact acccgccagg gtgatgacac
    16681 gcatactctc tccttataac caattgttct tttttttgtt aacattgata acaattcggt
    16741 cagtctgtcg gagagaca
//
LOCUS       DADWTH010000087        16621 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.088, whole genome shotgun sequence.
ACCESSION   DADWTH010000087 DADWTH010000000
VERSION     DADWTH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.088"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..746
                     /locus_tag="JNY02_002963"
     CDS             <1..746
                     /locus_tag="JNY02_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9434423.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JNY02_002964"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JNY02_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBB9434424.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="JNY02_002965"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="JNY02_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBB9434425.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="JNY02_002966"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="JNY02_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBB9434426.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..6724
                     /locus_tag="JNY02_002967"
                     /pseudo
     CDS             5041..6724
                     /locus_tag="JNY02_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            6776..7931
                     /locus_tag="JNY02_002968"
                     /pseudo
     CDS             6776..7931
                     /locus_tag="JNY02_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(7897..8019)
                     /locus_tag="JNY02_002969"
     CDS             complement(7897..8019)
                     /locus_tag="JNY02_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434427.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8222..9907
                     /locus_tag="JNY02_002970"
                     /pseudo
     CDS             8222..9907
                     /locus_tag="JNY02_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            9945..12317
                     /locus_tag="JNY02_002971"
     CDS             9945..12317
                     /locus_tag="JNY02_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBB9434428.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12374..15157
                     /locus_tag="JNY02_002972"
     CDS             12374..15157
                     /locus_tag="JNY02_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBB9434429.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15519..>16621
                     /locus_tag="JNY02_002973"
     CDS             15519..>16621
                     /locus_tag="JNY02_002973"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBB9434430.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT         4800 a         3672 c         3683 g         4466 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
     5221 atcgtactaa ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
     5281 gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa
     5341 gccattgaag ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta
     5401 cgttttacta acggctatgt ggcacacggt gtttccggcc cctcccgtgc cgcaataatg
     5461 accggtcgag ctcccgcccg cgttggtgtc tattccaata ccgatgctca ggatggtatt
     5521 ccgctaacag aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca
     5581 gtaggtaaat ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg
     5641 cgtgactatc atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt
     5701 ggctttgatt actttatggg attccacgct gcaggaacgg catattacaa ctccccttca
     5761 ctgttcaaaa atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat
     5821 gaggcaattg gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg
     5881 gcttataatg ctccgcacct accaaatgat aatcctgcac cggatcaata tcagaagcaa
     5941 tttaataccg gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag
     6001 ggtgtaaaac gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt
     6061 ctctttacct ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa
     6121 aaaggctata agagtcagac ctatcctggc ggtactcacc accccaatgt ttatgtggtg
     6181 gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg atttctaccc
     6241 aacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg atggtgtttc
     6301 cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc tgacctggat
     6361 aacctcttat tctcactggt ttgatgagga aaatattcca ttctgggata attaccacaa
     6421 atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg acttaagcca
     6481 attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag aaaacaatca
     6541 gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg ccgccaatcc
     6601 gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca gccagccacc
     6661 gcttagcgag gtaaatcagg agaagtttaa caatatcaag aaagcactaa gcgaagcgaa
     6721 ataactaaac cttcatgcgg cggatttttc cgccgcctta ttgagcgaga tagcaatgca
     6781 cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact gtttttacct
     6841 tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc tgaaagagtt
     6901 catcaaacaa tatatcgaag cgtctggcaa tcaggtctat tttacctggc aaggcggtga
     6961 acccactctg gctggcctgg attttttccg taaagttatt cactatcaac aacgctatgc
     7021 aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga ataatgaatg
     7081 gtgtgccttt ctcaaagaac atgattttct ggttggtatc tcgatcgatg gcccccagga
     7141 gttacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa aagtgatagc
     7201 agccatcgag cgtctgaaat catatcaagt agagtttaat acgttaaccg tcattaataa
     7261 cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg gcagtaaaca
     7321 tatgcaattt atcgaattgc tagaaaccgg gacgccgaat attgatttca gtggtcatag
     7381 tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg gcaagtttat
     7441 gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt
     7501 tgaaagcttt gtcagccgtt ttttggggaa tgggcataca agttgtattt tccaggagtc
     7561 ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg accattttgt
     7621 ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt
     7681 acaactgaca gcgcaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     7741 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     7801 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     7861 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     7921 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     7981 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     8041 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     8101 ccataaccac tgaaaattaa aaatttctat atatttcata ttgttaaatg atacttaaat
     8161 aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct
     8221 aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa
     8281 gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc
     8341 aatgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt
     8401 caatgcgcta agccagaaag aaaccgataa gctttggcag cttgttcttt ggttccctgc
     8461 cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac
     8521 tattcgctgg cgcgaatggt taacagatta ctaccttaat cgctggtttg cagataaaaa
     8581 ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat
     8641 cgctgaagac attctcttac tgatcaacaa aacgctcagt ctttcatttg gcttcatcca
     8701 gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc
     8761 attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata ccgtcgtgct
     8821 cattgtgatc ggtggaactt tatttaccca taaggtgggt aaacgtattc gtccgcttaa
     8881 tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat cttgtgcagc ataataagca
     8941 ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa
     9001 ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta
     9061 ctggcaaaat atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca
     9121 gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct
     9181 agtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc
     9241 ggttatcgat cgcttgtatg agttccatca actcactgaa tagcgcccta cgaataagcc
     9301 taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc ctgataataa
     9361 gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat tactgaaagg
     9421 ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct ggccgtgatt
     9481 taaaggtgat atttcttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa
     9541 aagcggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc
     9601 gttgagcgaa gtactgcatc aggttggtct ggggaaattg gctgcgcgta ttcatgacca
     9661 cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt
     9721 aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca
     9781 agaggctata cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat
     9841 ggttacacat caacccggtg tctggaacct ggctgatgat atttgtgaca ttagcacggt
     9901 tttataacaa caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta
     9961 ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt
    10021 acatgcattt ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca
    10081 acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg
    10141 atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc
    10201 agggagatat tcgccctgag aaaatctcta ttcacggtgc gtcgccctac cagaatgcct
    10261 atttgattga cggtattagt gcaactaata acctgaaccc agcgaatgag tccgatgcca
    10321 gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg
    10381 acaatgtgac gctttatgac agttttgtgc cggttgaatt tggtcgcttc aatggcgggg
    10441 taattgatgc aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc
    10501 gcactacgcg tttggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta
    10561 atcaaggttc ttcaggaagt acctatttct ctccagattt taaaaagaac ttttatacct
    10621 tgtcgtttaa tcaggagctg gctgataact ttggcgttac cgccggttta tcgcgccgcc
    10681 agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac
    10741 agtataaaaa cgttatcgat actgcattga gcaaatttac ctggtttgcc agcgaccgct
    10801 ttacccacga tttaacctta aaatataccg gctccagccg tgattataat accagcacct
    10861 tcccgcaatc tgatcgcgaa atgggtaata aatcctatgg tctggcatgg gatatggata
    10921 cgcaactcgc atgggccaaa ctacgtacca ccgttggttg ggatcatatt agtgattata
    10981 cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag
    11041 ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca
    11101 aaacacgcct ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctacttcg
    11161 gcgcggaata catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg
    11221 tgattaatgc tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc
    11281 tgggaattga caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt
    11341 taatgcccgg tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc
    11401 gctttatgac ggaatgggat atttttgctg atcaaacctc aatgattacc gccggttata
    11461 accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga
    11521 cggaatcggt atcaggtaat aaaaccctga cgcgttatca gaatttgaaa acgccttata
    11581 acgatgaact ggcaatggga ttgcagcaaa aaatcgataa gaacgttatt gcacgcgcaa
    11641 actatgttta ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
    11701 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
    11761 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
    11821 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
    11881 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
    11941 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
    12001 gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc
    12061 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
    12121 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
    12181 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
    12241 ttgatacaac gaacaccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
    12301 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
    12361 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
    12421 ccccgggcgg ctgattgccg ccgccttaca gcaggatgaa aagttaatta ccgggcaact
    12481 ggacaatggc ttgcgatata tgatttatcc gcatgctcat ccaaaggatc aggtaaattt
    12541 atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca
    12601 ttttgtagaa catatgatgt ttaacggcgc aaaaacatgg ccgggtaata aagtcatcga
    12661 aacatttgag tcaatgggcc tgcgttttgg tctcgatgtt aatgcctata ccagctatga
    12721 cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat
    12781 ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga
    12841 acgtggcgta atttctgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca
    12901 ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct
    12961 gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta
    13021 tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaag aagcgctggc
    13081 gctgataaag gataatttaa gtaagtttcc ggctaacaaa gcagctgaaa atcgcgtctg
    13141 gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt
    13201 gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt
    13261 tgtcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg
    13321 catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc
    13381 acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgcaggatgc
    13441 tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga
    13501 actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc
    13561 tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc
    13621 gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt
    13681 gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca
    13741 aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct
    13801 ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc
    13861 gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa
    13921 tctgacatca ttaacacttt ccaatggtgc caaggttatt ctggcaaaat ccgcgggtga
    13981 agagcaaaag ctacaaatta ttgccgtatc gaataaaggc gatttaagtt tccctgcgca
    14041 gcaaaaatca cttatcgcgc tggcaaataa agcagttagc ggaagcggcg ttggcgaact
    14101 ctcctcttcc agcctgaaac gctggagtgc ggaaaactcg gtaaccatga gcagtaaagt
    14161 cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt
    14221 ccagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct
    14281 acaaaatgct caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc
    14341 ccagctgatg tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca
    14401 gattgcacag tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc
    14461 agcggatatc acgtttgtca ttgtcggtaa tgtcgcagaa gacaaactcg tggcgttaat
    14521 tacgcgttac ttaggctcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt
    14581 aactcgcgcg acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca
    14641 ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc
    14701 gctcgatgct tttaacgtcg cactggcaaa agatctacgc gttaatattc gtgaacaggc
    14761 atctggggca tacagcgttt cttctcgcct ctcggttgat cctcaggcta aagatatcag
    14821 tcatttgctg gcttttactt gtcaaccaga acgacatgat gaacagttaa cgttagcgaa
    14881 tgaagtgatg gttaagcgtt tgactaaggg gatcagtgag caagaactga acgaatacca
    14941 gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac
    15001 tattataaat agtcttattc aatatgacga tcctgcagca tggactgagc aggagcaatt
    15061 gttgaaacaa atgacgatag agaatgttaa cactgccgtt aaacaatatc tttctcatcc
    15121 ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt
    15181 tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa
    15241 ataataacca agtactaata gtgatatttt aatgtctgat ttttacgtga taattcagga
    15301 ggcacagaaa gcgcataaaa ataacaacat aaaacacctt gccaccaccc aagaattctc
    15361 tattgcattg tttttaatga aaaaaatatc attccaacaa tatctcacga taaataacat
    15421 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
    15481 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
    15541 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
    15601 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
    15661 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
    15721 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
    15781 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaacatggtt gccgatctgt
    15841 ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg
    15901 aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg
    15961 caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtgcaaatc tgctggcata
    16021 aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt
    16081 ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc gtggtgccga
    16141 ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac gatgcactgg
    16201 ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc gccagcggtg
    16261 gctttctggc accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga
    16321 aatcgatcag tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta
    16381 tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctcggtg
    16441 gtcagattgg tacttttgcc atcaacttct cccgcccggc gggtcaggtg attgcacagt
    16501 actatgaatt cctgcgcctt ggtcgtgaag gctataccaa agtacagaat gcttcctacc
    16561 aggtcgctgc ctatctggcg gatgagatcg ccaaactggg gccgtatgag ttcatctgta
    16621 c
//
LOCUS       DADWTH010000088        16507 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.089, whole genome shotgun sequence.
ACCESSION   DADWTH010000088 DADWTH010000000
VERSION     DADWTH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.089"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..78)
                     /locus_tag="JNY02_002974"
     CDS             complement(<1..78)
                     /locus_tag="JNY02_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HBB9434431.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLI"
     gene            complement(283..987)
                     /locus_tag="JNY02_002975"
     CDS             complement(283..987)
                     /locus_tag="JNY02_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBB9434432.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNY02_002976"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNY02_002976"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBB9434433.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNY02_002977"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNY02_002977"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBB9434434.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNY02_002978"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNY02_002978"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBB9434435.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNY02_002979"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNY02_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBB9434436.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNY02_002980"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNY02_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBB9434437.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="JNY02_002981"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="JNY02_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBB9434438.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNY02_002982"
     CDS             complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNY02_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBB9434439.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNY02_002983"
     CDS             complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNY02_002983"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBB9434440.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPSSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNY02_002984"
     CDS             complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNY02_002984"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBB9434441.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNY02_002985"
     CDS             complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNY02_002985"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBB9434442.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNY02_002986"
     CDS             complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNY02_002986"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBB9434443.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11614..12903
                     /gene="bioA"
                     /locus_tag="JNY02_002987"
     CDS             11614..12903
                     /gene="bioA"
                     /locus_tag="JNY02_002987"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBB9434444.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12881..13351
                     /locus_tag="JNY02_002988"
     CDS             12881..13351
                     /locus_tag="JNY02_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBB9434445.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13905..14906
                     /locus_tag="JNY02_002989"
     CDS             13905..14906
                     /locus_tag="JNY02_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBB9434446.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14940..>16507)
                     /locus_tag="JNY02_002990"
     CDS             complement(14940..>16507)
                     /locus_tag="JNY02_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBB9434447.1"
                     /translation="FPNLLKYDPDQFGPDLIEQLAQSGKYSQDNTKGDAMIGVKQPLP
                     KAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQNAIDAVASLPSGGELFIPASNQA
                     VGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGSVLRLSYDSDTIGRYLRNIRVTGN
                     NTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVETAYLMQGLWHSKFIACQAGTCRVG
                     LHFLGQCVSVSVSSCHFSRGNYSADESFGIRIQPQTYAWSSEAVRSEAIILDSETMCI
                     GFKNAVYVHDCLDLHMEQPDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGI
                     YFRTPTSTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNI
                     VDINECVSFDKCIFNKPLCYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSV
                     NGIINVPVAVGATSGSAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSPV
                     GVALPSMVEYLAI"
BASE COUNT         3937 a         4341 c         4084 g         4145 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttccgtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atctaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
     6241 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
     6301 cgccaatttg tggaatggtg acgtgagatt catcgacgac cagcagccca tcggcaggca
     6361 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
     6421 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
     6481 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg
     6541 cggccagttc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
     6601 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
     6661 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
     6721 catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct
     6781 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
     6841 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
     6901 ccagaccata aatcgcggaa acagacgcca ctacaatcac atcacgccgc tccagcatcg
     6961 ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
     7021 tgaaggtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga
     7081 aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
     7141 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
     7201 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
     7261 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
     7321 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
     7381 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
     7441 atcaccataa tatttctgat acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
     7501 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
     7561 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgtacag tgactgccat
     7621 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
     7681 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
     7741 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
     7801 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
     7861 ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aaatacagaa
     7921 tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
     7981 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     8041 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
     8101 tccggcgtgt tgtattacct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     8161 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
     8221 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     8281 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgggc
     8341 gctgatgatg tgcggcgagg agggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8401 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
     8461 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
     8521 cgcggcttgt aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt
     8581 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8641 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8701 ttcgcgggtc gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg
     8761 cactgagcgc atcatcaaac catagtgtga tgagctgaat atgatgttga taacgcacgc
     8821 cgttcagcga ctcttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
     8881 ccaccgcctg ccgcgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8941 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
     9001 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     9061 gggattcgat atctcccgcc agataatgat ctgcggcatc tttatggcgg gcttgcgcaa
     9121 gcattggcgg cgagagatct aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc
     9181 tcatccagcc aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtggaagca
     9241 ttgccagtaa ggcgtcagca ctctggcgct gtagatctgc atgttgctca tagtgtgcgg
     9301 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9361 agcagacgat cgatatcctg catttcatgc gcagcggtta gcgttaagcg cagtcgcgca
     9421 gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt
     9481 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
     9541 catgaatcag ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaacg cgcaatgagt
     9601 gacaccagtt tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagcgacgca
     9661 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
     9721 tgcagcagat aatccgccac cgtacttgag caaagcaccg cagccccact gacgccaaat
     9781 cctttgccaa aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg
     9841 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
     9901 tgctgttgcg ttacctgctg gatttccgcc agtggcgcgc tatcgccgtc catgctgaac
     9961 acgccttctg tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
    10021 tgagcgacat cgttatgagt aaaacggcga agctgcgacg ggcttaaact ggcagcctcc
    10081 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
    10141 gcaatgactg cctgattcgc ggcaaaacca gagataaaca gcagcgcccg cgaatagcca
    10201 agccactcgg ccagttcttc ttccagcgcc tgatgcacca cgctataacc gctgacgtga
    10261 ccggagccgc cgctaccgat gccaaattgc tccgccccct gcttccaggc acggataatt
    10321 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    10381 tccgccacca gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg
    10441 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    10501 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg tccaagacgt tgctgttgtt
    10561 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10621 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    10681 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10741 cagaaaggcg cacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtgcgaataa
    10801 aatcaaaggc atcaacgtca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca
    10861 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    10921 cgcgatcttt taccgtttcg cctaagccca caatgccacc tgagcagact ttgatcccgg
    10981 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgtgtg gtgatgatat
    11041 tgccgtaaaa ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg
    11101 cgaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    11161 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    11221 tccatgccgc gcccatacag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact
    11281 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11341 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaacgtgc
    11401 tgacctgcac ctgacgagga tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca
    11461 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11521 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag gcttgtaaac
    11581 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    11641 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg
    11701 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11761 tcgtcctggt gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag
    11821 acgcaaattg atgccatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc
    11881 gagctatgcc gcaaactggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg
    11941 gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc
    12001 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    12061 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    12121 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgc
    12181 gatatggttg gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc
    12241 attgagccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12301 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12361 ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt
    12421 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac acttaccacg
    12481 cgcgaggttg cagaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12541 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc
    12601 ggcgaatggc agcagcaggt ggcggctatt gaagtgcagc tgcgcgagca actggcacca
    12661 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa
    12721 accactcgtc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggggtatgg
    12781 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12841 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    12901 taatggcgat aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa
    12961 tatttcaccg catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac
    13021 ctgctacgac ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt
    13081 acccgctgat acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga
    13141 cggcgttttg cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc
    13201 gaaaggcgaa acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga
    13261 tgtcgatgaa ggaggcagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc
    13321 aagcgcatcg attaccgcga tgtttagtta atcactctgc cagatggcgc aatgccatct
    13381 ggtatcactt aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa
    13441 gttagtataa aaaagctgaa cgagaaacat aaaacgctgt aaatatcaat atgttatgaa
    13501 agatttagtc taaataatag actgcataat actacaaaac acaacatatc cagtcactat
    13561 gaatcaacta cttagatagt attagtgacc tgaggcagag cattagcgca aggtgatttt
    13621 ttgtcctctt gcgctaattt tttgtcaacg gactgggtta gttgaattta caaccatgct
    13681 aagtatctcg ataagcgcag agaaatgatg cagtggtggg cggattggct tgatgagaag
    13741 gttaggtagg caaatccatg cctgagcaga gccttgccct ctacctgagc ccatgctcat
    13801 tctttggcct caacgcgagc atgacctcac tggctggacc caaaatggac tatgacagaa
    13861 agatctgata tttttcctcg caaaaactta gaggctttat tttaatgcat aagagcaact
    13921 gctttgacac ggcaaggctt gtggcagcta tgatggttct ggtcagtcac cactacgcgc
    13981 tctctgggca gccggagccg tacctgttcg gctttgaaag tgctggcggc attgcagtta
    14041 tcatattctt ttcaatatca ggatatctga tttcaaaatc agctatcagg agtgattcct
    14101 ttattgattt catggctaaa agagcgagaa gaatattccc ggcgctggtc ccttgctcta
    14161 tactgacata ttttttattt ggatggatac ttaacgactt cagcgcagaa tattttagcc
    14221 atgacattgt caggaagacc ataagctcta tttttatgag tcaggctcca gatgctgaca
    14281 ttacatcaca tttaattcat gccggaatta acggcagttt gtggacccta ccgcttgagt
    14341 tcctgtgtta tataataacg ggagtagcag ttgccctact aaagaacgga aaagcattca
    14401 tagttatact gttggttttt gtttctcttt cattaattgg aagtgtgtca gaaaacaggg
    14461 atgtaatgtt ctctattccg ttatggctgt atcctttgcg tggactggcg ttcttttttg
    14521 gtgccactat ggcaatgtat gagaagtcat ggaatgtaag taatgttaaa attactgtag
    14581 tttctttgct tgctatgtac gcctacgcta gttatggcaa aggcattgat tatactatga
    14641 cttgctatat cctggtttca ttctcgacaa tagccatatg cacatccgtt ggcgacccac
    14701 tggttaaggg caggtttgat tactcgtatg gtgtgtacat ttatgcattc ccggttcagc
    14761 aggttgttat aaacacattg cacatgggtt tttacccgtc aatgttgtta agtgccgtga
    14821 ctgttctttt cttgtcccat ctttcatgga atctggttga aaaaagattc ctgacacgaa
    14881 gttcgcctaa attatccctg gattgaacaa taaaaggggc catatggccc ctaaaataat
    14941 cagatggcca gatactcaac cattgaaggc agcgcgactc ctactgggct tgggcgagta
    15001 acattaattg tcaccacgga aacactaacc ttgtcaccac ttgatgcagg gtcaccaaaa
    15061 aggcttctta ctctgtatgt caggttcccc gggttcggta tagctgcaga tccgcttgtg
    15121 gcacctaccg caactgggac attaatgatc ccatttacag acggtacgcc tgaacagcca
    15181 cgatacgtat tgtatctgcc ttccggtttc tgcacctcgg taatgccagc caaataacag
    15241 tcagtaactg acacgccacc gctacgaaga tagcatagag gcttattgaa tatgcactta
    15301 tcgaaagaaa cacattcatt gatgtcgaca atatttacag cccattcatc accagttaac
    15361 gtacaaccac ttacaaagac gaagatcgcc gactgttcta tcgctatact ttgattgttt
    15421 gcagccgtgt ttttgtttgc agtgttgata tgaacaccac tgacaatttt atgtgactgc
    15481 gttgaggtcg gcgttctaaa atatatcccc gtaaattgtt cagtgccatc ggcatctgct
    15541 gctatccatg agtttgagaa agaaaatcct ccgtttacat tctctataac cacgcctgtt
    15601 gaaccgcaat aatctaaatc cggttgttcc atatgcaaat caaggcaatc atgaacatag
    15661 acggcatttt taaaaccaat gcacatggtc tcactgtcta aaattattgc ttctgaccta
    15721 actgcctctg acgaccacgc atacgtttga ggctgaatcc tgatgccaaa gctttcatca
    15781 gcagaataat ttcctctgct gaaatggcag gagctgacac taacacttac gcactggcct
    15841 aaaaagtgaa gaccgactct acaggttcca gcctgacatg ctataaattt tgagtgccag
    15901 agcccttgca ttaaataagc ggtttcaact tcattcacca ttacattatc aaatacccag
    15961 ccataaacct gtctgatgac agagtcttct gctgtaatgt ttgtgtcaat accattgcag
    16021 gtgttattac cagttactcg tatatttctc agataacggc cgatagtgtc tgaatcataa
    16081 gacagcctta acacagatcc tgtaagtccg ctttttgctc ggagttgcga tgccttacca
    16141 acccctctga tgttaacacc gccaggaata agcaaagtgg atccaacaat ataccccacc
    16201 gcttggttgc tcgcagggat aaacagctcc ccgccggagg gtagtgaagc aacggcatct
    16261 attgcatttt gtattgcctg gtaattatct gtcacaccat catcaataac accaaaatcc
    16321 aggatactta ttgcttcttt atttttgtca tgctgagttc ttaaaactgc ttttggtaaa
    16381 ggctgcttga cgccaatcat ggcatcgcct ttggtgttat cctgcgaata cttaccggat
    16441 tgagctagtt gctcaattaa atccggaccg aactgatcag ggtcatactt cagcaaattc
    16501 ggaaaat
//
LOCUS       DADWTH010000089        16325 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.090, whole genome shotgun sequence.
ACCESSION   DADWTH010000089 DADWTH010000000
VERSION     DADWTH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.090"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="JNY02_002991"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="JNY02_002991"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBB9434448.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENALPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JNY02_002992"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JNY02_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBB9434449.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="JNY02_002993"
     CDS             2341..2556
                     /locus_tag="JNY02_002993"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434450.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="JNY02_002994"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="JNY02_002994"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBB9434451.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /gene="galP"
                     /locus_tag="JNY02_002995"
     CDS             4451..5845
                     /gene="galP"
                     /locus_tag="JNY02_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBB9434452.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="JNY02_002996"
     CDS             5922..6419
                     /locus_tag="JNY02_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBB9434453.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="JNY02_002997"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="JNY02_002997"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBB9434454.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="JNY02_002998"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="JNY02_002998"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBB9434455.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="JNY02_002999"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="JNY02_002999"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBB9434456.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9104..9667
                     /locus_tag="JNY02_003000"
     CDS             9104..9667
                     /locus_tag="JNY02_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBB9434457.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="JNY02_003001"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="JNY02_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBB9434458.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="JNY02_003002"
     CDS             complement(10259..11239)
                     /locus_tag="JNY02_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBB9434459.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="JNY02_003003"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="JNY02_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBB9434460.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="JNY02_003004"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="JNY02_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBB9434461.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="JNY02_003005"
     CDS             12542..12832
                     /locus_tag="JNY02_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBB9434462.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="JNY02_003006"
     CDS             12840..13433
                     /locus_tag="JNY02_003006"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBB9434463.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAEMTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="JNY02_003007"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="JNY02_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBB9434464.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGMQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="JNY02_003008"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="JNY02_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..>16325
                     /locus_tag="JNY02_003009"
     CDS             15884..>16325
                     /locus_tag="JNY02_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBB9434465.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFY"
BASE COUNT         4042 a         4204 c         4110 g         3969 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctgcgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agagcatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc tacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacactacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgaatcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa aaaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccagggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg tcgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggtc tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cagcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ttgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcacttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taaccatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggtgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatagccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact cgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 ggccgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 gctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggcgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgatca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tataccatgg tgctgttatt acgcatctgg atgcagtggg
    12061 ctcattgtga tttttacaac cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgtc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aaatgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 tcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtatg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg cacaaagcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tgacgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa ggcaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg atgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 tacccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccgc gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatg
//
LOCUS       DADWTH010000090        16030 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.091, whole genome shotgun sequence.
ACCESSION   DADWTH010000090 DADWTH010000000
VERSION     DADWTH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.091"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="JNY02_003010"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="JNY02_003010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBB9434466.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JNY02_003011"
     CDS             1020..1703
                     /locus_tag="JNY02_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBB9434467.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JNY02_003012"
     CDS             1700..2161
                     /locus_tag="JNY02_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBB9434468.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JNY02_003013"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JNY02_003013"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBB9434469.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JNY02_003014"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JNY02_003014"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBB9434470.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSRAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNY02_003015"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNY02_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBB9434471.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="JNY02_003016"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="JNY02_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBB9434472.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="JNY02_003017"
     CDS             5380..6210
                     /locus_tag="JNY02_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBB9434473.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYFGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNY02_003018"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNY02_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBB9434474.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="JNY02_003019"
     CDS             complement(7339..8799)
                     /locus_tag="JNY02_003019"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBB9434475.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGSAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNY02_003020"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNY02_003020"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB9434476.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="JNY02_003021"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="JNY02_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBB9434477.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNY02_003022"
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNY02_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBB9434478.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNY02_003023"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNY02_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBB9434479.1"
                     /translation="MKWCKRGYVLAAMLALASATIQPADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNY02_003024"
     CDS             complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNY02_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBB9434480.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="JNY02_003025"
     CDS             complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="JNY02_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBB9434481.1"
                     /translation="SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSG
                     SKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRG
                     FAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGS
                     TQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYG
                     GTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNES
                     GTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGK
                     LQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAW
                     QKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED
                     NNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVL
                     AHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVAL
                     DTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSE
                     SSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAE
                     CR"
BASE COUNT         3861 a         4136 c         3923 g         4110 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tgactgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccgg gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattattt tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcactac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcggcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcataacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctggctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
    13741 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
    13801 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
    13861 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
    13921 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
    13981 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
    14041 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
    14101 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
    14161 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
    14221 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
    14281 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
    14341 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
    14401 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
    14461 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
    14521 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
    14581 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
    14641 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
    14701 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
    14761 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
    14821 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
    14881 tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
    14941 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
    15001 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
    15061 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
    15121 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    15181 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    15241 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    15301 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    15361 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    15421 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    15481 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    15541 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    15601 cggcatagat atcattgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    15661 atccattttg tttaatagtg acctgtgcag taccatgagc aataccgtgg atcaccgggg
    15721 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    15781 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    15841 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    15901 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    15961 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    16021 tctgtacact
//
LOCUS       DADWTH010000091        15954 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.092, whole genome shotgun sequence.
ACCESSION   DADWTH010000091 DADWTH010000000
VERSION     DADWTH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.092"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..445
                     /gene="tuf"
                     /locus_tag="JNY02_003026"
     CDS             <1..445
                     /gene="tuf"
                     /locus_tag="JNY02_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBB9434482.1"
                     /translation="IKETQKSTCTGVEMFRKLLDEGRAGENVGILLRGIKREEIERGQ
                     VLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVE
                     MVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            628..822
                     /gene="bfd"
                     /locus_tag="JNY02_003027"
     CDS             628..822
                     /gene="bfd"
                     /locus_tag="JNY02_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBB9434483.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            895..1371
                     /gene="bfr"
                     /locus_tag="JNY02_003028"
     CDS             895..1371
                     /gene="bfr"
                     /locus_tag="JNY02_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBB9434484.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1368..1835)
                     /locus_tag="JNY02_003029"
     CDS             complement(1368..1835)
                     /locus_tag="JNY02_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBB9434485.1"
                     /translation="MDTTLPFLILYACLSDLLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2214..2525
                     /gene="rpsJ"
                     /locus_tag="JNY02_003030"
     CDS             2214..2525
                     /gene="rpsJ"
                     /locus_tag="JNY02_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBB9434486.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2558..3187
                     /gene="rplC"
                     /locus_tag="JNY02_003031"
     CDS             2558..3187
                     /gene="rplC"
                     /locus_tag="JNY02_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBB9434487.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3198..3803
                     /gene="rplD"
                     /locus_tag="JNY02_003032"
     CDS             3198..3803
                     /gene="rplD"
                     /locus_tag="JNY02_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBB9434488.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3800..4102
                     /gene="rplW"
                     /locus_tag="JNY02_003033"
     CDS             3800..4102
                     /gene="rplW"
                     /locus_tag="JNY02_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBB9434489.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4120..4941
                     /gene="rplB"
                     /locus_tag="JNY02_003034"
     CDS             4120..4941
                     /gene="rplB"
                     /locus_tag="JNY02_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBB9434490.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4958..5236
                     /gene="rpsS"
                     /locus_tag="JNY02_003035"
     CDS             4958..5236
                     /gene="rpsS"
                     /locus_tag="JNY02_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBB9434491.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5251..5583
                     /gene="rplV"
                     /locus_tag="JNY02_003036"
     CDS             5251..5583
                     /gene="rplV"
                     /locus_tag="JNY02_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBB9434492.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5601..6302
                     /gene="rpsC"
                     /locus_tag="JNY02_003037"
     CDS             5601..6302
                     /gene="rpsC"
                     /locus_tag="JNY02_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBB9434493.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6315..6725
                     /gene="rplP"
                     /locus_tag="JNY02_003038"
     CDS             6315..6725
                     /gene="rplP"
                     /locus_tag="JNY02_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBB9434494.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6725..6916
                     /gene="rpmC"
                     /locus_tag="JNY02_003039"
     CDS             6725..6916
                     /gene="rpmC"
                     /locus_tag="JNY02_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBB9434495.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6916..7170
                     /gene="rpsQ"
                     /locus_tag="JNY02_003040"
     CDS             6916..7170
                     /gene="rpsQ"
                     /locus_tag="JNY02_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBB9434496.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7335..7706
                     /gene="rplN"
                     /locus_tag="JNY02_003041"
     CDS             7335..7706
                     /gene="rplN"
                     /locus_tag="JNY02_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBB9434497.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7717..8031
                     /gene="rplX"
                     /locus_tag="JNY02_003042"
     CDS             7717..8031
                     /gene="rplX"
                     /locus_tag="JNY02_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBB9434498.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8046..8585
                     /gene="rplE"
                     /locus_tag="JNY02_003043"
     CDS             8046..8585
                     /gene="rplE"
                     /locus_tag="JNY02_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBB9434499.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8600..8905
                     /gene="rpsN"
                     /locus_tag="JNY02_003044"
     CDS             8600..8905
                     /gene="rpsN"
                     /locus_tag="JNY02_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBB9434500.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8939..9331
                     /gene="rpsH"
                     /locus_tag="JNY02_003045"
     CDS             8939..9331
                     /gene="rpsH"
                     /locus_tag="JNY02_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBB9434501.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9345..9878
                     /gene="rplF"
                     /locus_tag="JNY02_003046"
     CDS             9345..9878
                     /gene="rplF"
                     /locus_tag="JNY02_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBB9434502.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9888..10241
                     /gene="rplR"
                     /locus_tag="JNY02_003047"
     CDS             9888..10241
                     /gene="rplR"
                     /locus_tag="JNY02_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBB9434503.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10256..10759
                     /gene="rpsE"
                     /locus_tag="JNY02_003048"
     CDS             10256..10759
                     /gene="rpsE"
                     /locus_tag="JNY02_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBB9434504.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10763..10942
                     /gene="rpmD"
                     /locus_tag="JNY02_003049"
     CDS             10763..10942
                     /gene="rpmD"
                     /locus_tag="JNY02_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBB9434505.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10946..11380
                     /gene="rplO"
                     /locus_tag="JNY02_003050"
     CDS             10946..11380
                     /gene="rplO"
                     /locus_tag="JNY02_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBB9434506.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11388..12719
                     /gene="secY"
                     /locus_tag="JNY02_003051"
     CDS             11388..12719
                     /gene="secY"
                     /locus_tag="JNY02_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBB9434507.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12751..12867
                     /gene="rpmJ"
                     /locus_tag="JNY02_003052"
     CDS             12751..12867
                     /gene="rpmJ"
                     /locus_tag="JNY02_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBB9434508.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13014..13370
                     /gene="rpsM"
                     /locus_tag="JNY02_003053"
     CDS             13014..13370
                     /gene="rpsM"
                     /locus_tag="JNY02_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBB9434509.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13387..13776
                     /gene="rpsK"
                     /locus_tag="JNY02_003054"
     CDS             13387..13776
                     /gene="rpsK"
                     /locus_tag="JNY02_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBB9434510.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13810..14430
                     /gene="rpsD"
                     /locus_tag="JNY02_003055"
     CDS             13810..14430
                     /gene="rpsD"
                     /locus_tag="JNY02_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBB9434511.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14456..15445
                     /gene="rpoA"
                     /locus_tag="JNY02_003056"
     CDS             14456..15445
                     /gene="rpoA"
                     /locus_tag="JNY02_003056"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBB9434512.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15486..15869
                     /gene="rplQ"
                     /locus_tag="JNY02_003057"
     CDS             15486..15869
                     /gene="rplQ"
                     /locus_tag="JNY02_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBB9434513.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4108 a         3755 c         4250 g         3841 t
ORIGIN      
        1 tatcaaagag actcagaagt ctacctgtac tggcgttgaa atgttccgca aactgctgga
       61 cgaaggccgt gctggtgaga acgtaggtat tctgctgcgt ggtatcaaac gtgaagaaat
      121 cgaacgtggt caggtactgg ctaagccggg caccatcaag ccgcacacca agttcgaatc
      181 tgaagtgtac attctgtcca aagatgaagg cggccgtcat actccgttct tcaaaggcta
      241 ccgtccgcag ttctacttcc gtactactga cgtgactggt accatcgaac tgccggaagg
      301 cgtagagatg gtaatgccgg gcgacaacat caaaatggtt gttaccctga tccacccgat
      361 cgcgatggac gacggtctgc gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg
      421 cgttgttgct aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc
      481 ttcggcgccc tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc
      541 gctgaactat ggcgcaaata aaactgtgct attgtaatca taaccattct catttacact
      601 ttgtgcagaa tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac
      661 aaaaaaattc gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag
      721 tttattcctg tgggaaatca atgcggtaag tgcattcgcg ccgcgcgcga agtgatgcag
      781 gacgagttga tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt
      841 gacatcccgc tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa
      901 ggtgatgtta aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc
      961 aatcagtatt ttctccatgc ccggatgttt aaaaactggg gcctgatgcg tctcaatgac
     1021 gtcgaatacc atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt
     1081 ttgtttttag aaggaatccc caacttacag gatttaggca aactggggat tggtgaagat
     1141 gtcgaggaga tgctgcaatc tgacctgcga ttagaactgg aaggcgcgaa ggatctgcgt
     1201 gaggctatcg catatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag
     1261 atccttgctg atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt
     1321 aaaatcggcc tacaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc
     1381 agcaaactat cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc
     1441 gggtttttta atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg
     1501 aatgaggcag caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg
     1561 gcggcgagaa acttcacatc accatagcct aatccttctt tatgtcgcag tatgcgatac
     1621 ccccagtaaa tgacagcgaa tgtgccgtaa ccaacaattg ccccccataa cgcatcggct
     1681 aaaccatcag gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta
     1741 aatctgtcag gtaacaagcc atgctttgca tcccagaaaa aaagtaggtc tgataaacaa
     1801 gcgtacaaaa ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat
     1861 caccatcgaa taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt
     1921 ccggtgaaaa acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg
     1981 cgcgttgcga ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc
     2041 gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc
     2101 taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa
     2161 tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga
     2221 accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg
     2281 cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc
     2341 cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg
     2401 atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa
     2461 ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg
     2521 gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa
     2581 agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga
     2641 agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat
     2701 tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt
     2761 cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga
     2821 agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga
     2881 cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg
     2941 tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca
     3001 gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga
     3061 acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct
     3121 ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa
     3181 ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt
     3241 ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt
     3301 atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt
     3361 ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga
     3421 gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa
     3481 aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc
     3541 aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg
     3601 cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg
     3661 aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta
     3721 tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc
     3781 aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc
     3841 gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt
     3901 tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga
     3961 agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat
     4021 cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga
     4081 cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa
     4141 ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc
     4201 aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc
     4261 cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc
     4321 aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt
     4381 tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct
     4441 aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt
     4501 aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa
     4561 ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt
     4621 gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac
     4681 tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa
     4741 gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtactgc gatgaacccg
     4801 gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg
     4861 tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc
     4921 gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg
     4981 tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa
     5041 gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac
     5101 catcgctgtc cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg
     5161 tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa
     5221 agcgaagaag aaataaggta ggaggaagag atggaaacta ttgctaaaca tcgccatgct
     5281 cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg
     5341 caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtacaggt caagaaagtt
     5401 ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa
     5461 gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa
     5521 ggtcgtgcag atcgtatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc
     5581 tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta
     5641 ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg
     5701 acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc
     5761 gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg
     5821 gtatcgttat cggtaagaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca
     5881 tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa
     5941 aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta
     6001 tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta
     6061 gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac
     6121 cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg
     6181 gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg
     6241 ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat
     6301 aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc
     6361 gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg
     6421 ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg
     6481 cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa
     6541 agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga
     6601 ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag
     6661 cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga
     6721 tgtaatgaaa gcaaaagagc tgcgtgagaa gagcattgaa gagctgaaca ccgagctgct
     6781 gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca
     6841 gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga
     6901 gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac
     6961 aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt
     7021 aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc
     7081 ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg
     7141 gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg
     7201 gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg
     7261 cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt
     7321 agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg
     7381 cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag
     7441 gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg
     7501 atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg
     7561 tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg
     7621 gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta
     7681 tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat
     7741 gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc
     7801 ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg
     7861 gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc
     7921 aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa
     7981 gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta
     8041 gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg
     8101 agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg
     8161 gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag
     8221 caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa
     8281 tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt
     8341 tctttgagcg cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg
     8401 ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc
     8461 cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta
     8521 ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca
     8581 agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt
     8641 agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt
     8701 gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga
     8761 ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct
     8821 gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc
     8881 gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat
     8941 gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa
     9001 caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa
     9061 ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact
     9121 tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc
     9181 aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat
     9241 cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct
     9301 tggtggcgaa attatctgct acgtagccta aatcggagga aaaaatgtct cgtgttgcta
     9361 aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag gttattacga
     9421 tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa gttaaacatg
     9481 cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg gcacaggctg
     9541 gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc ttcactaaga
     9601 agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg attaacctgt
     9661 ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact gctgaatgtc
     9721 cgactcagac tgaaatcgtg ctgaaaggcg ctgataagca ggtgatcggc caggttgcag
     9781 cggatctgcg cgcctaccgt cgtcctgagc cttacaaagg caagggtgtt cgttacgccg
     9841 acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg gataagaaat
     9901 ctgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg ggcgcaactc
     9961 gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca ccgaacggtt
    10021 ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa ctgaagtaca
    10081 ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc gctctggaaa
    10141 aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt cgtgtccagg
    10201 cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt gtaagatggc
    10261 tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa accgcgtatc
    10321 taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag ttggcgatgg
    10381 taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag cgatccagaa
    10441 agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg gcactctaca
    10501 acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg cttccgaagg
    10561 taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg gggttcataa
    10621 cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg caactattga
    10681 tggcctagaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta aatccgttga
    10741 agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa cccgcagtgc
    10801 aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ttgggtctgc gtcgtattgg
    10861 tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg cggtttcctt
    10921 catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg ccgaaggctc
    10981 caaaaaggcg ggtaaacgcc tggggcgtgg tatcggttct ggcctcggta aaaccggtgg
    11041 tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg gtttcgaggg
    11101 tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc gtaaagcagc
    11161 gattacagcc gaagttcgtc tatctgacct ggctaaagta gaaggcggtg ttgtagacct
    11221 gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga aagtgatcct
    11281 ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta aaggcgctcg
    11341 tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg gctaaacaac
    11401 cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc agactgctgt
    11461 ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc cctggtattg
    11521 atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt gagatgttta
    11581 acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg atcatgccgt
    11641 atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg ttggcagaaa
    11701 ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgt tacggtactc
    11761 tggtgctggc aatattccag tcgatcggta ttgctaccgg tctgccgaat atgcctggta
    11821 tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt gtaagtctgg
    11881 tcacaggaac gatgttcctg atgtggttgg gcgaacagat cactgaacga ggtatcggca
    11941 acggtatttc aatcattatc ttcgccggta ttgtcgcggg actcccgcca gccattgccc
    12001 atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg ttggttgcag
    12061 tattagtatt tgcagtgacg ttctttgttg tgtttgttga gcgtggtcaa cgccgcattg
    12121 tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgtcta tgctgcacag agcacacatt
    12181 taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc agtattattc
    12241 tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac tggctgacaa
    12301 caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat gcgtctgcaa
    12361 tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa acagcagata
    12421 acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa acggcgaagt
    12481 atatcgataa agtaatgacc cgcctgacct tggttggtgc gctgtatatt acctttatct
    12541 gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc ggtgggacct
    12601 cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa actctgatga
    12661 tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac ggccgataat
    12721 tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt caagaaatta
    12781 tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg cagtgccgag
    12841 ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca aagttgggtt
    12901 gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat ttgagtatcc
    12961 tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc atagtggccc
    13021 gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta acttcgattt
    13081 atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc gctgaagatg
    13141 ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa gttgccaaat
    13201 ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc ctgatggatc
    13261 ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt cagcgtacca
    13321 agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa tcggggtgat
    13381 tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca agtctctgac
    13441 ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac tgatcgtcag
    13501 ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc tcgcaaatcc
    13561 actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa agaatacggc
    13621 atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc tactattcgt
    13681 gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc gatccctcat
    13741 aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca ggtttgttgg
    13801 agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg tgagggcacc
    13861 gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat tgaacaagct
    13921 cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca gttgcgtgaa
    13981 aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa ctactacaaa
    14041 gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct ggaaggtcgt
    14101 ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc acgtcagctg
    14161 gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc ttatcaggtt
    14221 agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg cgtgaaagcc
    14281 gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga tgctggtaag
    14341 atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga cattaacgaa
    14401 cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg acacaatgca
    14461 gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag tgagttcgac
    14521 gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc tgggtaacgc
    14581 actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg ttgagattga
    14641 tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc tggaaatcct
    14701 gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta ttcttacctt
    14761 gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg gtgatgtcga
    14821 aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt ctattagcat
    14881 gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa ttcattcgga
    14941 agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc ctgtggagcg
    15001 tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg acaagctggt
    15061 catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc gtgcggcaac
    15121 cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc agcctgaagt
    15181 gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg atctggaatt
    15241 gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg gtgatctggt
    15301 acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat ctcttactga
    15361 gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcttgg aaaactggcc
    15421 accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact gagaaggata
    15481 aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc catcgccagg
    15541 ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag acgactctgc
    15601 ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc aagactgata
    15661 gcgttgctaa tcgtcgtctg gcattcgccc gtactcgtga taacgagatc gtggcaaaac
    15721 tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact cgtattctga
    15781 agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg gttgatcgtt
    15841 cagagaaagc agaagctgct gcagagtaat ctgaagcaac gtaaaaaaac ccgccccggc
    15901 gggttttttt atacccgtag tatccccact tatctacaat agctgtactc tttt
//
LOCUS       DADWTH010000092        15952 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.093, whole genome shotgun sequence.
ACCESSION   DADWTH010000092 DADWTH010000000
VERSION     DADWTH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.093"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNY02_003058"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNY02_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBB9434514.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JNY02_003059"
     CDS             complement(998..2020)
                     /locus_tag="JNY02_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9434515.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JNY02_003060"
     CDS             complement(2034..3536)
                     /locus_tag="JNY02_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBB9434516.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNY02_003061"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNY02_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="JNY02_003062"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="JNY02_003062"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBB9434517.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="JNY02_003063"
     CDS             complement(5853..6194)
                     /locus_tag="JNY02_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBB9434518.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNY02_003064"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNY02_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBB9434519.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNY02_003065"
     CDS             complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNY02_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBB9434520.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11912..12550
                     /gene="msrA"
                     /locus_tag="JNY02_003066"
     CDS             11912..12550
                     /gene="msrA"
                     /locus_tag="JNY02_003066"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB9434521.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12873..14216
                     /locus_tag="JNY02_003067"
     CDS             12873..14216
                     /locus_tag="JNY02_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBB9434522.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14278..14484)
                     /locus_tag="JNY02_003068"
     CDS             complement(14278..14484)
                     /locus_tag="JNY02_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBB9434523.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKMHLSVMSEVNRQVMRLQTEMA"
     gene            14809..15363
                     /locus_tag="JNY02_003069"
     CDS             14809..15363
                     /locus_tag="JNY02_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBB9434524.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15426..>15952)
                     /locus_tag="JNY02_003070"
     CDS             complement(15426..>15952)
                     /locus_tag="JNY02_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434525.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3796 a         4371 c         3921 g         3864 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agcagacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtcctt tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgtg ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agctaccacc accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa
    11041 atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    11101 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    11161 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    11221 ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc
    11281 ataatcgccc tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata
    11341 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    11401 cacacctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    11461 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc
    11521 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    11581 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    11641 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    11701 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    11761 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    11821 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    11881 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    11941 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    12001 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    12061 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc
    12121 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    12181 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    12241 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    12301 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    12361 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat
    12421 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    12481 tctgcataaa aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc
    12541 ggaagcatag cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc
    12601 tgatgcggct taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag
    12661 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    12721 attttaaccc tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg
    12781 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt
    12841 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    12901 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca
    12961 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    13021 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    13081 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    13141 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    13201 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    13261 aagcggtggc tttgcgtatc atcaatccga tgcgcttctg cctgtacgtt tgcaccccgc
    13321 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    13381 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    13441 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    13501 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    13561 atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    13621 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    13681 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    13741 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    13801 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga
    13861 ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    13921 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    13981 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    14041 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    14101 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    14161 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    14221 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    14281 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    14341 catcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    14401 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    14461 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    14521 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    14581 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    14641 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    14701 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    14761 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    14821 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    14881 atcggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    14941 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg
    15001 caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    15061 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    15121 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    15181 ccctggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    15241 gaagaaagtt ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac
    15301 ggggcgctca caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa
    15361 taatggcaat aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag
    15421 gggaattaag tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt
    15481 ttaaacgcct caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta
    15541 tctgcggcca gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg
    15601 caacctgcat taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca
    15661 gaggaaccat tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat
    15721 tctatgacta tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcgcat
    15781 tctttaattc caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact
    15841 acatcctcca ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct
    15901 ttaatccttt ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc at
//
LOCUS       DADWTH010000093        15442 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.094, whole genome shotgun sequence.
ACCESSION   DADWTH010000093 DADWTH010000000
VERSION     DADWTH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.094"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..290
                     /locus_tag="JNY02_003071"
     CDS             <1..290
                     /locus_tag="JNY02_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBB9434526.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            290..5560
                     /gene="traI"
                     /locus_tag="JNY02_003072"
     CDS             290..5560
                     /gene="traI"
                     /locus_tag="JNY02_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBB9434527.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5580..6326
                     /gene="traX"
                     /locus_tag="JNY02_003073"
     CDS             5580..6326
                     /gene="traX"
                     /locus_tag="JNY02_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBB9434528.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6381..6941
                     /locus_tag="JNY02_003074"
     CDS             6381..6941
                     /locus_tag="JNY02_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBB9434529.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7073..7285
                     /locus_tag="JNY02_003075"
     CDS             7073..7285
                     /locus_tag="JNY02_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBB9434530.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7531..7992
                     /locus_tag="JNY02_003076"
     CDS             7531..7992
                     /locus_tag="JNY02_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBB9434531.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8038..8247
                     /gene="hha"
                     /locus_tag="JNY02_003077"
     CDS             8038..8247
                     /gene="hha"
                     /locus_tag="JNY02_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBB9434532.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            8285..8875
                     /locus_tag="JNY02_003078"
     CDS             8285..8875
                     /locus_tag="JNY02_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBB9434533.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            9030..9503
                     /locus_tag="JNY02_003079"
     CDS             9030..9503
                     /locus_tag="JNY02_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434534.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            9750..9899
                     /locus_tag="JNY02_003080"
     CDS             9750..9899
                     /locus_tag="JNY02_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBB9434535.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            10184..10432
                     /gene="repA"
                     /locus_tag="JNY02_003081"
     CDS             10184..10432
                     /gene="repA"
                     /locus_tag="JNY02_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBB9434536.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            10677..10751
                     /gene="tap"
                     /locus_tag="JNY02_003082"
     CDS             10677..10751
                     /gene="tap"
                     /locus_tag="JNY02_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBB9434537.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10744..11601
                     /gene="repA"
                     /locus_tag="JNY02_003083"
     CDS             10744..11601
                     /gene="repA"
                     /locus_tag="JNY02_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBB9434538.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            12539..13192
                     /locus_tag="JNY02_003084"
     CDS             12539..13192
                     /locus_tag="JNY02_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB9434539.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            13285..13542
                     /gene="pemI"
                     /locus_tag="JNY02_003085"
     CDS             13285..13542
                     /gene="pemI"
                     /locus_tag="JNY02_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HBB9434540.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            13475..13876
                     /gene="pemK"
                     /locus_tag="JNY02_003086"
     CDS             13475..13876
                     /gene="pemK"
                     /locus_tag="JNY02_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HBB9434541.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            14161..>15442
                     /locus_tag="JNY02_003087"
     CDS             14161..>15442
                     /locus_tag="JNY02_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBB9434542.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQ"
BASE COUNT         3909 a         3593 c         4467 g         3473 t
ORIGIN      
        1 cacaacagcc agtgtcttct gtcatcaatg ataagaagtc agatgcaggt gtgagtgttc
       61 cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac
      121 tgccacccgg gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc
      181 aacaggaaaa tcatccggac atccagcagc atatgcagcg tcgtgaagag gtgaacatta
      241 atgtgcaccg ggagcgcggg gaggatgttg agccgggaga tgatttctga tgttgagttt
      301 cagcgttgtt aaatcggcag gcagtgccgg aaactattac accgacaagg ataattacta
      361 tgtgctgggc agcatgggag aacgctgggc cggtcagggg gctgaacagc ttgggctgca
      421 gggcagcgtc gataaggatg tttttacccg tcttctggag ggcaggctgc cggacggggc
      481 ggatttaagc cgcatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt
      541 ctccgccccc aaaagtgtct ccatgatggc catgctcggg ggggataaac gtcttattga
      601 tgcacataac caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg
      661 ggtgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
      721 taaccatgac accagtcgcg atcaggaccc acagttacac acgcatgtgg tggtggctaa
      781 tgtcacgcag cataacggcg agtggaagac actgagcagt gacaaagtgg ggaaaaccgg
      841 attttccgag aacgtgctcg cgaaccggat tgcgttcggg aaaatatatc agagtgagtt
      901 acgtcagcgc gttgaggcgc tgggttatga aaccgaagtg gtcggtaagc acggtatgtg
      961 ggagatgccg ggcgtaccgg tggaggcctt ttccagtcgt tcacaggcta tcagggaggc
     1021 cgtcggtgaa gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc
     1081 caaacagcac gtcgatccgg aaatcagaat ggctgaatgg atgcagacgc tgaaggaaac
     1141 cgggttcgac atccgggcat atcgtgacgc ggcggatcag cgtgcggaga tccgcacgca
     1201 ggcgcccggg cctgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggcgat
     1261 tgccggatta agtgaacgaa aagtgcagtt cacgtatacg gacgtgctgg ccaggacggt
     1321 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     1381 catcagccgt gagcagctta tccccctcga ccgtgagaag gggctgttca catcaggaat
     1441 tcatgtgctc gatgagctgt ccgtccgggc actcagtcgt gacatcatga aacagaaccg
     1501 ggtgaccgta catccggaga aaagtgtccc ccggacggct ggttacagcg atgcggtcag
     1561 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     1621 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gaacaggggc gggaggtgca
     1681 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     1741 tgaactgata accggacgtc gtcagctgct ggaaggcatg gccttcacgc cgggcagtac
     1801 cgttatcgtc gaccagggtg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     1861 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
     1921 cagtgcgctg atggccatga aggatgccgg ggtgaacaca tatcgctggc agggcggaga
     1981 acagcgaccg gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc
     2041 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggggt
     2101 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacgc agggcgtgct
     2161 cggacaccct gaggtgacca tgactgcact gtcaccggtc tggctggaca gccggagccg
     2221 ttatctgcgg gatatgtacc gaccggggat ggtgatggag cagtggaacc cggagacacg
     2281 cagtcatgac cgctatgtta tcgaccgggt gacggcgcag agtcacagcc tgaccctgcg
     2341 ggatgcgcag ggcgaaacgc aggtggtgcg tatttcctcc ctggacagca gctggtcgct
     2401 gttccggccg gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat
     2461 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     2521 gatgacggtt gttgtgccgg ggcgggctga accggccacc ctgcctgtgg ctgattcacc
     2581 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagtga
     2641 cagtgcgacg gtgtttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg
     2701 tctggcccgc agcggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     2761 ggaaaaactc gcccgccatc cgtcctttac ggtggtttct gagcagataa aggcgcgtgc
     2821 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     2881 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat
     2941 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttactgaact
     3001 gggaggggaa atcaatgcgc agataaaacg cggtgactta ctgtatgtgg atgtggcaaa
     3061 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     3121 tcgccatatt ctcgaaggca aggaggcggt cacgccgctg atggagagag tacccggcga
     3181 actcatggag acgttaacgt cgggacagcg tgccgccacc cgtatgatac tggaaacgtc
     3241 cgaccgtttc acggtggtac aggggtatgc cggtgtgggt aagaccactc agttccgggc
     3301 ggtgatgtca gccgtgaaca tgctgccgga gagtgagcgt ccccgggttg tggggctggg
     3361 gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc gtggatgcac agacactggc
     3421 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     3481 cacgctgttt ctgctcgatg agagctcaat ggtgggcaat accgacatgg cacgggcata
     3541 cgccctgatt gcggccggtg gcggtcgtgc tgtggccagc ggtgacacgg accagctgca
     3601 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc
     3661 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     3721 taaccgggat gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc
     3781 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     3841 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatat
     3901 ccccatgaca ctgtatgagg ccattgtccg cgactatacc ggcaggacac cggaagcacg
     3961 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4021 tcatgatgca cgggaaaagg ccggtgagct gggcaaagag caggtcatgg tgcctgtcct
     4081 gaacacagcg aatatacgtg acggagaact gcgtcgtctc tccacctggg agaaaaaccc
     4141 ggatgccctt gccctggtgg atagtgtgta tcaccggatt gccggtatca gcaaggatga
     4201 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     4261 ggtggctgaa ggtgtcacac tgtacacccc ggacaaaatc cgggtgggga ccggcgaccg
     4321 gatgcgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     4381 gacagcggtt tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg
     4441 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ctgcccacgg
     4501 tgcgcagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     4561 acagatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     4621 ggtgtacacc gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg
     4681 aactgcccac gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cgcagcggct
     4741 gttcagtacg gcacgggaac tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     4801 ggggctggcc gggggagaca gtccggcacg gtttattgca ccggggcgca aatatccgca
     4861 gccgtatgtg gcactgcctg cttttgaccg taacggcaag tccgccggta tctggctgaa
     4921 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gacgggtgaa
     4981 aggcagcggg gatgcgcagt ttgtggccct gcagggcagc cgtaacggcg agagcctgct
     5041 ggctgataat atgcaggatg gtgtccggat tgcccgtgat aaccccgaca gcggtgtggt
     5101 ggtgagaatc gccggtgaag gtcgtccgtg gaatcccggt gccatcaccg gtggtcgcgt
     5161 gtggggggat atcccggaca acagcgttca gccgggagcc ggaaatggcg aaccggtcac
     5221 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     5281 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     5341 agagcaggct gtcagggaga ttgccggaca ggagcgtgac cggactgcca cttctgaacg
     5401 ggaagccgcg ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg
     5461 ggaggttgcc cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt
     5521 tcgtgacctg cagaaagaga aaaccctggg cggagactga tacaggaaga taaacgctga
     5581 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     5641 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     5701 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     5761 ttctggccgg gcgaggagcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc
     5821 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatggggg attattgccc
     5881 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     5941 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6001 ccattcttct gatggccctg tgggggcctt tgtccggcac cagttacggc attgccgggc
     6061 tgctgatgct ggcagtcagc caccggctgt accgggcgga agacagagcg gaacgtctgg
     6121 cgctggtcgc ctgtctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     6181 cggcggtggc tggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     6241 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     6301 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     6361 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgaa
     6421 aaacggaagg ggagacgcct gtccgcaggc ggaaaaccat catcaatgtc accacgccac
     6481 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcggagctgg
     6541 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg ccctcgctgg
     6601 atgaggccgt gaacaccctg aaaccctggt ggccgggatt atttgacggt gaaacgcccc
     6661 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     6721 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     6781 tgtgtgccat gaaagccggt gcctgccggt atgatacgga agggtatgtg acggagcata
     6841 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaaca
     6901 ggataaaggc agagcttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     6961 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg tacccgaaag gaagccaatt
     7021 cagtagatta tttaacacca taatactgac gggttaaaga ggagtgataa agatgaatgg
     7081 attcagaaac agttcacgga acggtcaggt ctggcgctgg cagcgtgccg gaagccgggc
     7141 cgttattctg gaggtcagtg gacgctggat ggaagcggca gaagcatggc gacgggctgc
     7201 cggtgttgcg ccccggacag actggcagca gtttgcccga aaaagggctg aacactgcca
     7261 ccggcgcagc cggggaaggg tgtaacgaaa aaagcagcct tcattactcc agggattcag
     7321 aaggctgaat aatgcatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga
     7381 cagttctgag tagcacagag ttttttccgt attaagtcgt gattttatat tttgtgatta
     7441 atttcacaaa ataaggtgtt attcagtgtg tgctgcgata ttcaggatta cctgaaatac
     7501 aggtgccatt tatctgatat ggagaataac atgaggaaat atattccact ggtattattt
     7561 atcttttcat ggccggtatt gtgtgcagat attcatggac gggttgttcg tgtactggac
     7621 ggtgatacca tcgaggtcat ggactcacgg aaggcagtgc ggatcaggct ggtaaatatt
     7681 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cggacatgat gaaatcactg
     7741 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
     7801 gggcaggttt atgcaccaga cggaatgaat gttaatcagt ttatggtccg ggccggggct
     7861 gcctgggtat atgagcagta caacacagat cccgtgctgc ctgtccttca gaacgaagcc
     7921 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggaga
     7981 caccgtaaat aaagcgatgg caatcttatc attcatctgt tctcagcggg aataaaaatg
     8041 gcgaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
     8101 gaaaaaatta ttcataaaaa cagagacagt ttattaaaca gtgaacgtga atcatttaat
     8161 tcagcagcag accaccggtt agcggaatta atcaccggaa agttatatga caggatacca
     8221 aaagagatat ggaaatatgt aaggtaataa ttcagaataa ttaattacca ggagcagata
     8281 aacaatgaag ctgattattt ttattctcat tgttctgatt attgcagcgt tacttatccg
     8341 gattatcctc aaaagtgtaa atcagcattc tcctttgctg atgcagttgc atgcggcagg
     8401 tatacggacc ggtgatgcag agcgaatact gtccggcggt gaatactggc aacgtcagaa
     8461 gacgctgctg acagaaaggg aagtcagttt tatgaaagga ctgttcagaa ttgtggatat
     8521 gaagcggtgg tatctgtgtc cgcaggtacg ggtcgcggat atcgtccagc tgaacgggaa
     8581 tatccggcca cgatcacgcc agtggtggca gttattcagg atggtgtctc agtggcatgt
     8641 tgatgtggtt atcgttgaac ggcgttcgtt cagtattgtt gcagcagtag agctggatga
     8701 tgccagccat ttacgaccgg aacgccggcg ccgggatatt cttctggaag aggttctgag
     8761 gcaggctggt attccgttgc tcagaagcca cgatgccaga aaactgctgc agatgaccgg
     8821 agaatggctg aatacaacag gggctgatca gcagtccccg gaacatcgta gctgacgcct
     8881 tcgcgttgct cagttgtcca accccggaaa cgggaaaaag caagttttcc ccgctcccgg
     8941 cgtttctgta ataaaaagcg ggaaatggtg gtcacagagt aaattccgga accacctgta
     9001 cccaaacttt atataataat ggtgaatata tgaaatatga tgatgtatca gaattgatga
     9061 ttaagcgtga acacaatatt cttattgttg ggaactctgc ctcccacatt gataagtttt
     9121 ttaaggggta ttctatacca ggttctaaat attatgattt cactcagatt aacagtgatt
     9181 cagatgtaga gagaaatgag gatgctgtct cctttatcag ggatgcaatg aatagtgaag
     9241 agcagacaat catttttaat tgtgctgggt ggcctgattt aggtggtgga tcagcagtat
     9301 cacagtttgc aatgatggtc agaaaatttg gtaaacaact tattgtcgct gtaagtgaaa
     9361 aggatgccat caaattaaaa gacaactttg atattattgg gatgttgtca tatggtaaag
     9421 aaaattttat tgccatgagt catacgaaaa acgagttaac tggaggaaaa cgtcggtacc
     9481 ggattaaaaa tggatgtaaa taaaaagcat acttattggc tggagtgacg ccttcgtgct
     9541 tctgtagtaa aaagcgggca atgcaggtaa tagtcagcaa cagggaatgt ggtattatcg
     9601 cggcgggtgt ctgagccttt ctggttcagg caagacgcag gtaccagaaa tgcaaagacc
     9661 ccacttgtta atccattaac tcgtgaggtc tgcatgaagt acattgcact actgattgta
     9721 gcctcttcct tgcagagagg tcaaagttta tgacgaagta tgcccttatc gggttgctcg
     9781 ccgtgtgcgc cacggtgttg tgtttttcac tgatattcag ggaacggtta tgtgagctga
     9841 atattcacag gggaaataca gtggtgcagg taactctggc ctacgaagca cggaagtaaa
     9901 ctgccgggcg gggacggaag tccccgcttt ccggaagtgt gaggtatttc aggggcagac
     9961 acccgacatg ccagaaacag ccggtcccgc ccggggccgg ctcccgggtt aaggcatttc
    10021 ctgttttttc agccatttcg caacaaaaat cacattaaac aacagtaatc cccgttgatt
    10081 tgtgcgccaa cacagatctt cgtcacaatt ctcaagtcgc tgatttcaaa aaactgtagt
    10141 atcctctgcg aaacgatccc tgtttgagta ttgaggaggc gagatgtcgc agacagaaaa
    10201 tgcagtgact tcctcatcag gtacaaagcg agcatacaga aaaggtaatc ctctgacgct
    10261 tgcagaacgt cagcaggcat ccctggcaag aaaacgagct acacataaag aactcagagt
    10321 gttcattcct gctgcgttaa aagcgcaatt gcaggtgatg tgtgaagctg agggcgttac
    10381 ccaggctgag atgattgccg aactgataaa gcagaagagt gcttttagct aactaaaatg
    10441 tagcgacatc acattcttgc ctgtttctgg tgtgcgtgat agattgctga tcgtttaagg
    10501 aattttgtgg ctggccacgc cgtaaggtgg cagggaactg gttctgatgt ggatttacag
    10561 gagccagaaa agtgaaaacc ccgataatct tcgacaagtt tggcgactga gaagattacc
    10621 ggggccatct aaaaaccgta tagccaacaa ttcagctatg cggggagtat agttatatgc
    10681 ccggaaaagt tcaagacttc tttctgtgct cactccttct gcgcattgta agtgcaggat
    10741 ggtgtgactg atcttcaaca aacgtattac cgccaggtaa agaacccgaa tccggtgttt
    10801 acaccccgta aaggtgccgg aacgctgaag ttctgcgaaa aactgatgga aaaggcggtg
    10861 ggcttcactt cccgttttga tttcgccatt catgtggcgc atgcccgttc gcgtggtctg
    10921 cgtcgacgca tgccaccggt gctgcgtcga cgggctattg atgcgctcct gcaggggctg
    10981 tgtttccact atgacccgct ggccaaccgc gtccagtgct ccatcaccac gctggccatt
    11041 gagtgcggac tggcgacgga gtctgctgcc ggaaaactct ccatcacccg tgccacccgg
    11101 gccctgacgt tcctgtcaga gctgggactg attacctacc agacggaata tgacccgctt
    11161 atcgggtgct acattccgac cgatatcacg ttcacatctg cactgtttgc tgccctcgat
    11221 gtatcagagg aggcagcggc cgccgcgcgc cgcagccgtg tggtatggga aaacaaacaa
    11281 cgcaaaaagc aggggctgga taccctgggc atggatgaac tgatagcgaa agcctggcgt
    11341 tttgttcgtg agcgttttcg cagttatcag acagagctta agtcccgggg aataaagcgt
    11401 gcccgtgcgc gtcgtgatgc ggacagggaa cgtcaggata ttgtcaccct ggtgaaacgg
    11461 cagctgacgc gcgaaatcgc ggaagggcgc ttcactgcca atcgtgaggc ggtaaaacgc
    11521 gaagttgagc gtcgtgtgaa ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg
    11581 ctggccacag cttccccctg aaagtgacct cctctgaata atccggcccg cgccggaggc
    11641 attcgcacgc ctgaagcccg tcgatgcaca aaaaaacagc accacataca aaaaacaacc
    11701 tcatcatcca gcttctggtg catccggccc cccctgtttt cgatacaaaa cacgcctcac
    11761 agacggggaa ttttgcttat ccacattaaa ctgcaaggga cttccccata aggttacaac
    11821 cgttcatgtc ataaagcgcc agccgccagt cttacagggt gcaatgtatc ttttaaacac
    11881 ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa cctattcact
    11941 gcctgtcctg tggacagaca gatatgcacc tcccaccgca agcggcgggc cccgaccgga
    12001 gccattttag ttacaacact caaatacaag caccgggaaa accccagtcc agcgcagaac
    12061 tgaaaccaca aagcccctcc tcataactga aaagcggccc cgccccggcc cgaagggccg
    12121 gaacagagtc gcttttaatt atgaatgttg taactacatc ttcatcgctg tcagtcttct
    12181 cgctggaagt tctcagtaca cgctcgtaag cggccctcac ggcccgctaa cgcggagata
    12241 cgccccgact tcgggtaaac cctcgtcggg accactccga ccgcgcacag aagctctctc
    12301 atggctgaaa gcgggtatgg tctggcaggg ctggggatgg gtaaggtgaa atctatcaat
    12361 cagtaccggc ttacgccggg cttcggcggt tttactcctg tatcatatga aacaacagag
    12421 tgccgccttc catgccgctg atgcggcata tcctggtaac gatatctgaa ttgttataca
    12481 tgtgtatata cgtggtaatg acaaaaatag gacaagttaa aaatttacag gcgatgcaat
    12541 gattcaaaca cgtaatcaat atctgcagtt tatgctggtt atgctggctg cctggggcat
    12601 tagttgggga gccagatttg tcatggagca ggccgttctg ctttatggat caggaaaaaa
    12661 ctatttgttc ttcagtcatg gtactgttct gatgtacctg ctgtgtgttt tcctggtata
    12721 ccgccgttgg atagctccgc taccggtcgt tggtcagctg cgcaacgttg gcgtaccgtg
    12781 gctggtcggt gcgatggccg tggtgtatgt cggtgtattt ctgctcggta aggcgctggc
    12841 tctgcctgct gagccattta tgacgaaact ttttgccgat aagtccatac ctgacgtgat
    12901 cctgacgttg ctgaccatct ttatccttgc cccgttgaat gaggaaacgc tgttccgggg
    12961 gattatgctg aacgtcttcc gttcacggta ctgctggacg atgtggctgg gggcgctgat
    13021 aacgtcgttg ttgttcgtcg ccgcgcacag ccagtatcag aacctgctga cactggcaga
    13081 actgttcctg gtggggttga ttacatcagt ggccaggatc agaagtggtg gcctgctgct
    13141 gccggtattg ctgcatatgg aagcaaccac gctgggttta ctgtttggtt gaaagttata
    13201 tttttattaa acattgtgcg ttaaagcctg gtgtgttttt ttagtggatg ttatatttaa
    13261 atataacttt tatggaggtg aagaatgcat accacccgac tgaagagggt tggcggctca
    13321 gttatgctga ccgtcccacc ggcactgctg aatgcgctgt ctctgggcac agataatgaa
    13381 gttggcatgg tcattgataa tggccggctg attgttgagc cgtacagacg cccgcaatat
    13441 tcactggctg agctactggc acagtgtgat ccgaatgctg aaatatcagc tgaagaacga
    13501 gaatggctgg atgcaccggc gactggtcag gaggaaatct gacatggaaa gaggggaaat
    13561 ctggcttgtc tcgcttgatc ctaccgcagg tcatgagcag cagggaacgc ggccggtgct
    13621 gattgtcaca ccggcggcct ttaatcgcgt gacccgcctg cctgttgttg tgcccgtaac
    13681 cagcggaggc aattttgccc gcactgccgg ctttgcggtg tcgttggatg gtgttggcat
    13741 acgtaccaca ggtgttgtac gttgcgatca accccggaca attgatatga aagcacgggg
    13801 cggaaaacga ctcgaacggg ttccggagac tatcatgaac gaagttcttg gccgcctgtc
    13861 cactattctg acttgaacat ggggtttgag gggcaactgg atgaaaacgt acgatttagg
    13921 gcactaaaac cgctgttgtc ccaccattct ggtgattccc aaacgttatt tggctaaaaa
    13981 gtagttttga tgtggtttat tttccgaatt ccaagcgcag ccctactttc ttgtgcgtat
    14041 ttgcgtgttt tgcgcagttt tgaagttcct acatcagatt gacttcggat acaaattcaa
    14101 tgaacattga tgcaaatact gacctatcag tttcttaact aaatactcga gatacatcta
    14161 atggctaatg aaaacttaga tattgactcc aagacagcag aagaattata cgagtggtat
    14221 ctggatgata aattaattat aaatcgaaga taccaaagaa aattagtatg gtccctcgaa
    14281 gagaaaacag cactgatttc atcaatgact cagcaatacc ctatcccttt gctattgttt
    14341 gtttccattg acaacaaaag agaaatcctt gatggaatgc aaagactaga agctttaatg
    14401 agttttattg agcagcgatt ttcattaaat ggtaaatact ttaatttaga tgccattgca
    14461 ttaacaaaac aactaaaaga cagtggtaaa cttcaacaaa aagagccagt gctatcccgc
    14521 gaaagttcta cattaattgc tcgctataga tttgctatat ctgaatactc ttcaacagag
    14581 gaccacattg acgaagtgtt tcgacgcata aattccaatg gtaaaatttt atcaaaacaa
    14641 gaactacgaa gtgcggggtg cgttagtaat ttttcagagt tagtaaggaa aatatcgaca
    14701 attataagag gagatactac acatagcgat ataatgggac tgaataaaat acataacata
    14761 tcaatttgca atgatggact cgattatgga ataaatatag acaatcattt ctatattaga
    14821 aatcatatca ttagtaggcc tagcattaga gactctgacg atgaagagtt agttgcaaat
    14881 atcttgggct atatattttt agatgataag ccaacatcag gatcaacatc gctcgataca
    14941 ttttacggtg aaggttcgac gagtcatgca atccatacac gaactcaatt agagaattac
    15001 attcagacaa atggtgcaga taaaattgtc aataattacc tcttcgttta tgagatgatt
    15061 caaaaattat ttgatgcaaa taatttgaac tttagatcac acattctggg caatgcatct
    15121 tcatctcaag aatgtcctcg ttactatcaa gctgtttttc ttgctttgta tgaattaatc
    15181 ataaatgaaa acatgcaact tgacgatgaa cagaaattta tcgctcagtt aggagacagt
    15241 gtacaacgtt caatggtgca aaccgaaggg ggccgttggg cagctagcgc tcgtcagaaa
    15301 agtgtcgaag atttatgtgc tttaatcaga cgatatttta aagaatcaga aaataaattt
    15361 atcaatcatg cttggcaaac actgataaga actctactta ataactcaag aacagaacaa
    15421 ccaaattatg attttaaaca gg
//
LOCUS       DADWTH010000094        15082 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.095, whole genome shotgun sequence.
ACCESSION   DADWTH010000094 DADWTH010000000
VERSION     DADWTH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.095"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            29..292
                     /locus_tag="JNY02_003088"
     CDS             29..292
                     /locus_tag="JNY02_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBB9434543.1"
                     /translation="MSKLESLPTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLV
                     FAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            302..940
                     /locus_tag="JNY02_003089"
     CDS             302..940
                     /locus_tag="JNY02_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBB9434544.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            943..2106
                     /locus_tag="JNY02_003090"
     CDS             943..2106
                     /locus_tag="JNY02_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBB9434545.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2190..3814
                     /locus_tag="JNY02_003091"
                     /pseudo
     CDS             2190..3814
                     /locus_tag="JNY02_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="JNY02_003092"
     CDS             complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="JNY02_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBB9434546.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="JNY02_003093"
     CDS             complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="JNY02_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBB9434547.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4866..5615
                     /gene="yjfP"
                     /locus_tag="JNY02_003094"
     CDS             4866..5615
                     /gene="yjfP"
                     /locus_tag="JNY02_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBB9434548.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="JNY02_003095"
     CDS             complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="JNY02_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBB9434549.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="JNY02_003096"
     CDS             complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="JNY02_003096"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBB9434550.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCANDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7894..9291
                     /gene="ulaA"
                     /locus_tag="JNY02_003097"
     CDS             7894..9291
                     /gene="ulaA"
                     /locus_tag="JNY02_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBB9434551.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9307..9612
                     /gene="ulaB"
                     /locus_tag="JNY02_003098"
     CDS             9307..9612
                     /gene="ulaB"
                     /locus_tag="JNY02_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBB9434552.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9622..10086
                     /gene="ulaC"
                     /locus_tag="JNY02_003099"
     CDS             9622..10086
                     /gene="ulaC"
                     /locus_tag="JNY02_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBB9434553.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAVVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10100..10750
                     /gene="ulaD"
                     /locus_tag="JNY02_003100"
     CDS             10100..10750
                     /gene="ulaD"
                     /locus_tag="JNY02_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBB9434554.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10760..11595
                     /gene="ulaE"
                     /locus_tag="JNY02_003101"
                     /pseudo
     CDS             10760..11595
                     /gene="ulaE"
                     /locus_tag="JNY02_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            11595..12281
                     /gene="ulaF"
                     /locus_tag="JNY02_003102"
     CDS             11595..12281
                     /gene="ulaF"
                     /locus_tag="JNY02_003102"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBB9434555.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTL
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12410..12685)
                     /locus_tag="JNY02_003103"
     CDS             complement(12410..12685)
                     /locus_tag="JNY02_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBB9434556.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13012..13407
                     /gene="rpsF"
                     /locus_tag="JNY02_003104"
     CDS             13012..13407
                     /gene="rpsF"
                     /locus_tag="JNY02_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBB9434557.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13414..13728
                     /gene="priB"
                     /locus_tag="JNY02_003105"
     CDS             13414..13728
                     /gene="priB"
                     /locus_tag="JNY02_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBB9434558.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13733..13960
                     /gene="rpsR"
                     /locus_tag="JNY02_003106"
     CDS             13733..13960
                     /gene="rpsR"
                     /locus_tag="JNY02_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBB9434559.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14002..14451
                     /gene="rplI"
                     /locus_tag="JNY02_003107"
     CDS             14002..14451
                     /gene="rplI"
                     /locus_tag="JNY02_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBB9434560.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14522..>15082)
                     /locus_tag="JNY02_003108"
     CDS             complement(14522..>15082)
                     /locus_tag="JNY02_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBB9434561.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     RTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3529 a         3671 c         4058 g         3824 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac ctacattgtt
       61 aggttacgtt atcccattat ccagtgcggc aatcaatgcg gtgagtattc cagactattt
      121 cgcctggggc gggattgcac tggtgattca gttactcgtt tttgctggtg tcagacttta
      181 tatgcctgca ttaagcgaaa aaattattaa tcacaatacc gcagcaggaa tgtttatggg
      241 aaccgccgcg ctggctggcg gtatttttaa cgcagcttgt atgacatggt aatggacgat
      301 catggccaga aaacgcaaat caagaaataa cagtaaaatt ggtcacggag cgatcagtcg
      361 cattggtaga ccgaataatc cttttgaacc gcgtcgcaat cgctacgcac aaaaatactt
      421 aacgttggcg ctaatgggcg gtgccgcttt tttcgtattg aaaggttgta gcgatagtag
      481 cgatgtcgat aacgacggcg acggaacgtt ttacgcgaca gtgcaggatt gtattgatga
      541 cggtaataat gccgacattt gcgcgcgtgg ctggaacaac gccaaagcgg cattttatgc
      601 cgatgttcca aagaatatga ctcagcagaa ctgtcagtct aagtacgaaa attgctacta
      661 cgacaatgtt gaacagagtt ggatcccggt cgtttctgga tttttgttaa gccgggttat
      721 tcgtaaagat cgcgatgagc cgttcgtcta taacagcggc ggatcctcat ttgcttcccg
      781 ccccgtctgg cgcaacactt ccggtgatta ctcctggcgc ttcggttctg gcaaaaaaga
      841 gtcttactct tcaggcggct ttaccaccag aaaagcgtcg accgtttcgc gcggcggcta
      901 tggtcgttct tccagcgccc gtgggcattg gggaggctaa tcatgctgag acacaacgtt
      961 cctgtgcggc gggatctgga ccagatcgcc gctaacaacg gtttcgactt tcatatcatc
     1021 gacaatgaaa tctattggga cgagagtcgg gcttaccgtt ttactctgcg ccagattgaa
     1081 gagcagatcg aaaaaccgac tgcggaactg catcagatgt gccttgaggt ggtggatcgc
     1141 gcggttaaag acgaagagat cctgacgcaa ctggcgatcc cgccgttgta ctgggatgtg
     1201 atcgccgaaa gctggcgcgc acgcgatcct tcgctgtatg gccggatgga ttttgcctgg
     1261 tgtggcaatg cgccggtgaa gctgctggag tacaacgccg atacgccgac ctcgttgtat
     1321 gagtcggctt atttccagtg gttgtggctg gaggatgccc ggcgcagcgg cattattccg
     1381 cgtgatgccg atcagtacaa cgccattcag gaacgcctga tttcgcgctt tagtgagctt
     1441 tacagtcggg aaccgtttta tttttgctgc tgtcaggaca ccgatgaaga caggagtacc
     1501 gtgctgtact tgcaggactg cgcccagcag gcagggcagg agtcgcggtt tatctacatt
     1561 gaagatctcg gtttgggcgt cggcggcgta ctgaccgatc ttgatgataa tgtcatccag
     1621 cgtgcattta agctgtatcc gctggagtgg atgatgcgtg acgataacgg tccgctgctg
     1681 cgcaagcgtc gcgagcaatg ggtggagccg ttatggaaaa gtatcttgag taataaaggg
     1741 ctaatgccgc tgctttggcg cttcttccct ggtcatccta accttcttgc atcctggttt
     1801 gagggtgaaa aatcgcagat tgccgctggc gaaagctatg tgcgtaaacc gatctactcg
     1861 cgcgaaggcg gaaacgtcac cattttcgac ggtcagaata acgtcgttga ccacgctgat
     1921 ggtgattacg ccgatgaacc gatgatctac caggcgtttc aacctctgcc gcgatttggc
     1981 gatagctaca cactcatcgg tagctggatt gtcgatgatg aagcgtgcgg aatggggatc
     2041 cgtgaagata acacactgat caccaaagac acctcacgtt tcgttccgca ttacattgct
     2101 ggataagaat gttttagcaa tctctttctg tcatgaatcc atggcagtga ccatactaat
     2161 ggtgactgcc attgatggag ggagacacag tgcactggca aactcacacc atttttaatc
     2221 aacctatacc attaaataac agcaacttat acctgtctga tggcgcgctt tgcgaagcgg
     2281 taacgcgtga aggtgctggc tgggatagcg attttctagc cagtattggt cagcagttag
     2341 gaacggctga atcccttgaa ctggggcggc tggcgaatgt aaacccgcct gaactgttgc
     2401 gctacgatgc ccaggggcgg cgtctggacg atgttcgttt ccatcccgca tggcacctgc
     2461 tgatgcaggc gttatgtacc aatcgggtgc acaatcttgc ctgggaagaa gacgctcgct
     2521 ccggcgcatt tgtggcgcgc gcggcgcgtt ttatgttaca tggcaggttg aggctgggtc
     2581 gttatgtcca ataacgatga cctttgccgc cacgccattg ttgttacaga tgttacccac
     2641 gccgtttcag gactggacca cgccgctgct gagcgatcgc tacgattctc acttattgcc
     2701 aggtgggcaa aaacgcggtt tgttgattgg catgggaatg acggaaaagc agggcggttc
     2761 cgatgtcatg agcaacacca tccgtgcaga gcgtttggaa gatggctctt atcggctggt
     2821 ggggcataaa tggtttttct cggtgccgca aagcgatgcg catctggtgc tggcgcagac
     2881 cgcgggcggt ctgtcctgct tttttgtgcc gcgctttttg cctgacgggc aacgcaacgc
     2941 gattcgcctc gagcggctga aagataagct gggtaatcgc tctaacgcca gttgcgaagt
     3001 ggagtttcag gatgccattg gctggttatt ggggcaggaa ggggaaggaa ttcgtctgat
     3061 cctgaaaatg ggggggatga cgcgttttga ttgcgccctg ggtagccatg ccatgatgcg
     3121 ccgtgcattt tcgctggcga tttatcatgc acatcaacgc catgtttttg gtaatccatt
     3181 gatccaacag ccccttatgc gtcatgtctt aagtcgcatg gcacttcagc ttgaagggca
     3241 aacggcgttg ctgtttcgtc ttgcgcgagc gtgggaccgg cgtgccgatg ccaaagaagc
     3301 cctgtgggcg cgtttattta cgcctgcggc aaaatttgtg atctgcaaac gaggtatgcc
     3361 gtttgtggcc gaagcgatgg aggtgctggg cggcattggt tattgcgagg agagcgagct
     3421 gccgcggctt taccgggaga tgccggtaaa cagtatttgg gaaggttccg gcaatattat
     3481 gtgcctggat gtgttgcgcg ttctcaataa gcaagcgggc gtatacgact tattgtcgga
     3541 agcatttgtg gaagtgaaag ggcaggatcg ctattttgat cgcgcggttc gtcgtttaca
     3601 gcagcagctg cgtaagccag ctgaagaact ggggcgagag attactcatc agctattcct
     3661 gctgggctgc ggtgcgcaaa tgttgaaata tgcttctccg ccaatggcgc aggcgtggtg
     3721 tcaggtgatg ttagatacgc gcggcggcgt acggttgtca gagcagatcc agaatgattt
     3781 attgctgcgg gcgacggggg gagtgtgtgt gtaagcgtat acgactgatg cgacgctggt
     3841 ttcgattaac taaatgaaat atgtgaaaat tgtaggccgg acaaggcgct cgcgccgcat
     3901 ccggcattgt tcatttgctg tagatgacac tcatgcatac agtatcgcct gtacgcgcca
     3961 gttatcaggc tgctggtctt cgtacatggt aataatgcgg taccatgacg caccttgttc
     4021 atcggccttt aacgccacga tacgttccac gtcctgcgga tcccctgaaa tattgctaac
     4081 tgagatctga cctatttcat tcaggcccgt cacgcagtca gcactggcga attctgcaga
     4141 ctgtgccgtg gcgctcgcag gataagtcac taattgcagc gagggtgagg caagtaattg
     4201 tttcatcgtc agctccattt ttcgtttccc cacatgttgc agggcattcc atcgcggtaa
     4261 taaacgccca agcttccagc cggtaattta ttgtgcacag gtaaacgcat agggacgcaa
     4321 agcaatgtaa agctgactat gtaagttgtc tgatttattt acggtacaaa atggcttgtg
     4381 aataccactg tcctgtcacg atggtttcgt ccaccatgac gacaacgtaa taatcagctt
     4441 ttgcagcgac agcttttgct ctgatttctg ctaatgcgtc atccggagaa ccccgaacca
     4501 tcgtgcttac gctacctatt cgctgtaacc cttgcgtctg gtcgcggcga atctcttgcg
     4561 gatggtccgt tactggcggt gctggctgtg gcgtaccttg cagtgcgcta caggcactta
     4621 acatcaacac caataataaa ctggcaaacc ggtaaataac gctattacgt ttcctgctaa
     4681 ccatagtgta gtgccttatt aatttaactt tgggggaatg ttcccgaatt gttacctggt
     4741 acgcgatttt cgaatgaaaa cgtgacgaaa gcgtaaccca catctcaaca ttatgaacta
     4801 ataccgccag cagaaacttg tgctaaggat ttcttcatgt cataccagac aaaaaggaga
     4861 gacagatgat tgaaatagaa tcacgcgagc tggcagatat tcccgttctt catgcttatc
     4921 ctgtcgggca aaaagatacc ccgttaccgt gcgtaatttt ttatcacggc tttacttcat
     4981 ccagcctggt gtatagctat tttgccgttg cgctggcgca ggctggtttg cgggtgatca
     5041 tgccggatgc gcccgatcac ggtagccgtt ttagtggcga cgaagcgcgg cggttaaatc
     5101 aattctggca aatcttgcta caaagtatgc aggaattcac tactttacgt gcggcaatag
     5161 ccgaagaaaa ctggctgctt gatgaccgtc tggcagtcgg tggcgcgtcg atgggcgcga
     5221 tgacggcact gggaattacc gctcgccacc ccacggtgaa atgtaccgcc agcatgatgg
     5281 gatcgggcta ttttacatcg ctcgcccgtt cactgtttcc accgctgata cctgaaacgg
     5341 cagcacagca gaatgaattc aataaaattg tcgcgccgct ggcagagtgg gaagcgacaa
     5401 accacctgga acaacttggg gacagacctc tactgctgtg gcatggcctc gacgatgacg
     5461 ttgtgcctgc cgacgaatca ctacgtttgc agcaggcctt aagcgagacg ggacgggata
     5521 aactgctaac ctgttcatgg cagccaggcg tgcgtcaccg cattacgcct gaggcgttag
     5581 atgctgccgt gacatttttc cgccagcatc tttaaacacg cagaatgctg accccttgcg
     5641 cttccagttg ttgcaggatt tccgggttag catttttgcc ggtgatcagc atatcgattt
     5701 gatcggcacg gctaaaaagc attcccgcgc gttcgccaat cttactgcta tcaaccagta
     5761 ccaccagttt ccctaccacg ctcagcatct tctgctctgc cattgctgtc agcatatcgg
     5821 ttttatacaa cccttctgcg gtcagccctt ttccgctggt aaacatccag tgcccggcat
     5881 agagactgtt ttcgctgccc tgcgggctta aagtgatgga ctgacttttg ttgtactgtc
     5941 cacccataat gatcacgctg tcatgttctt gatcgatcag gtaatttgcc agcggtagat
     6001 aattagtgat gatttgcact ggcttgccac acatttcccg cccaagcaga aacgcggtgg
     6061 agccgcagtt gatgactacg ctttcgcccg gattaaccag ctgcgaggcc gctttagcaa
     6121 tacgtacttt ttcatcgtga ttctgcgcct gatgcagatt catcggcgtc cagcgcgggc
     6181 gctgttgggt aatagcttct gcgccattgc gcaccttttt cagtttgccg ctttcgccaa
     6241 gtttattgat atcgcgtcgc gcagtggcag gcgaaattcc cagacgctca acgactttct
     6301 caacggtcac aaagcccaat tgtgcgagca tttccaggag gatttgatgt ctttgtgctt
     6361 cagtcatgag ctattccgat aaaatttgat ttttttagat gatatttgaa atagccagga
     6421 aatactacgc cggatagcgc gaacttctcc acgctatccg gtataaacga cagattacag
     6481 gaatgacttg aacggcagat ccggttcaat agtgaagcaa tcatcgaaac cgcgcggata
     6541 gtggtactcg aagttgtctt tatccagcgg ccaggtaaat ttgccgccaa cctgccagat
     6601 aaacggcttg aagccatact tcagacgatc ttttttcatc tcccacagca cgcggatctc
     6661 ttgcggatcg gcctggaagt ttgaccagat gtcgtggtgg aacgggataa ccactttcgc
     6721 attcagggct tcacccatac gcagcatatc ggcgctggtc attttgtcgg taataccgcg
     6781 cgggttctca ccatacgatc ctaacgccac atcgatctga tgttcgttac cgtgcttcgc
     6841 ataatagtta gagtagtggg aatcgccact gtgatatagg gaaccgccag gggttttgaa
     6901 caggtagttt actgcgcgat cgtccatgcc atctggcagt acgccagccg ctttttgatc
     6961 ggcaggcagg gtgatcagtg cagtacggtc gaaagcgtca agcgcatgaa tttcaatgtc
     7021 tttcactttt acaacatcgc ccggtttgac cacgatgcaa cgctctttcg gtacgcccca
     7081 gccaatccac aaatcaacgc aggttttcgg tccaatgaat ggtacgtcat tagcacagtt
     7141 ctgcatcacg gcagcagcga cgttaacgtc gatatgatcg ttgtgatcgt gagtcgccag
     7201 taccgcgtcg atctggcgaa tcgcaaacgg atcaagaaca aacggggtgg tgcgtaggtt
     7261 tggctgcagt tttttcacgc cagccatgcg ctgcatctgg tgaccctgtt tcattaacgg
     7321 gttaccgtga ctttgtttgc cagtgccgca ccagaaatca acgcaaacgt tggtgccacc
     7381 ttctgatttc aaccagatcc cggtgcagcc tagccaccac atcgcaaatg tgccaggggc
     7441 gacctgctct tgttcaattt cttcattcag ccagctaccc cactccggga aagtgctcag
     7501 gatccaggat tctcgggtga tacttttcac tttactcatc gccatttacc ttcatgatag
     7561 ttcaattcga atcaatatgt gattggtttt gattaatcct gacactattt tttcaggaag
     7621 gcaatgacca ttttttgact ttttgccagg gaagttgttg ttgattttga gtgtagaaag
     7681 atttaatgga atgtatagat aaattaacta aatgaattta aataaataat tttttcgttg
     7741 tgtgaatccc actctatccg tgtggaatta tttgcgggtc gcgccacatt taatcataaa
     7801 taatcttgtt gtgattactt ttgaaaatta gagtgagtgc acaacattcc gggtgtgcgg
     7861 aatacccggt tacctcttct tcaggagatc gttatggaga tcctctacaa catctttacc
     7921 gtgtttttta accaggtcat gaccaatgcc ccgttgttgc tgggtattgt gacctgtctg
     7981 ggctacatcc tactgcgcaa aagtgtcagc gttattatta aaggcacgat taaaaccata
     8041 attggtttca tgttgttgca ggcagggtcc ggcatcctca ccagcacctt caaaccggtg
     8101 gtggcgaaaa tgtccgaagt ctacggcatt aacggcgcaa tttccgatac ttacgcgtcg
     8161 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     8221 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     8281 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     8341 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8401 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8461 ttctccatcg gtcaccagca acagtttgca tcattaattg cctataaagt tgcgccgttc
     8521 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8581 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8641 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8701 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8761 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8821 gttctggcga ttgactgtgc ggctatttat agcttcgctc cgaacgccgt ggtctggggc
     8881 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8941 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     9001 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     9061 atcgaaatct ttggttgcgt ctgggtggtg aaacttaccg gtatgagtgc ctggatgggg
     9121 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     9181 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     9241 ctgcgcgcag aagaagatgc agaaaaacaa ttggcagaac agtctgctta ataaggagtt
     9301 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9361 atgaagatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9421 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9481 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9541 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9601 gatgtgaagt aaggacgcgc catgaaatta catgattcgc tggcggagaa taaatccatc
     9661 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9721 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9781 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9841 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9901 gaagataacg acccggtgga tatcctcatc accatggcgg tggtcgatgc caacacgcac
     9961 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
    10021 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
    10081 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
    10141 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
    10201 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
    10261 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
    10321 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10381 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10441 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10501 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10561 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10621 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10681 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10741 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10801 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10861 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10921 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10981 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    11041 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    11101 ccagctgcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    11161 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cacaggtgac gctggcgatg
    11221 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    11281 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    11341 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11401 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11461 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11521 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gcatggccaa agcaggcatg
    11581 gtggaggcgg cataatgcaa aagctaaaac agcaggtgtt tgaagccaac atggatctgc
    11641 cgcgctatgg gctggtgacc tttacttggg gcaacgtcag cgctatcgac cgcgaacgcg
    11701 ggctggtggt gatcaagccc agcggcgttg cctacgaaac catgaaagca gacgatatgg
    11761 tggtggtcga tatgagcggc aaggtggtgg aaggggagta tcgcccatct tccgacactg
    11821 cgacgcatct cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc catacccact
    11881 ccactcatgc caccgcatgg gcgcaggcgg ggctggcgat cccggcatta ggcaccacgc
    11941 acgctgacta cttctttggt gatatcccgt gtacgcgtgg attaagcgaa gaagaggtgc
    12001 agggtgagta tgaactgaac actggcaaag tgattatcga aacgctgggc aacgccgagc
    12061 cgctgcatac gctgggaatt gtggtgtatc agcacggacc tttcgcctgg gggaaagatg
    12121 cccacgacgc ggtgcataac gcggtagtga tggaagaagt ggcgaaaatg gcgtggattg
    12181 cgcgcagtat taatccacaa ctcaatcata tcgacagcta cctgatgaat aaacacttca
    12241 tgcgtaagca cggtcctaac gcttattacg ggcagaagta aaacacgcgc tgcggaaatt
    12301 tccttcctcg ggagataact ggtctaattc cgcagccgtt tttcaaaaaa agccccctgc
    12361 gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc tagcgataaa
    12421 tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga attacgtggt
    12481 agtacgttgc gccttgcgca tctgtttctt catttaatgc ctgacgtgct tcgctttcag
    12541 tggcgaaatt atgattgata taaatcacgc ctaagctttg cacatcgtcc atatttctgg
    12601 cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa agcacagcca
    12661 gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt gtgtgctctc
    12721 ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat ggtgaccatt
    12781 tctgatgcag ttgttcaaaa aaacactgtg acggaatgtg atgaacttca aatcagcggt
    12841 ttagaggtta tttgcgaaag gggagattta tttctgctcc gcccttgagt ttagcggggt
    12901 atgtcagtac tataacgcgt catttttcag ccgaccttta acacgttcct tgcctccccg
    12961 ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat
    13021 tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg catgatcgag
    13081 cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga agactggggc
    13141 cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt tctgatgaac
    13201 attgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt caacgatgcc
    13261 gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt
    13321 aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat
    13381 gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt gttgtccggc
    13441 accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca ctgccagttc
    13501 gtgcttgagc atcgttctgt gcaggaggaa gccggctttc accggcaggc atggtgtcaa
    13561 atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat aacggtcggc
    13621 agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg actgagcaaa
    13681 atggttttgc acgccgagca gattgaattg atagattctg gagactagcc atatggcacg
    13741 ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag agatcgacta
    13801 taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg tcccaagccg
    13861 tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca aacgcgctcg
    13921 ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg tccattaata
    13981 cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc aaacctgggt
    14041 agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag
    14101 ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg tcgcgctgaa
    14161 ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga gaaaatcaat
    14221 gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc
    14281 atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt ggctaagagc
    14341 gaagttcgtc tgccgaacgg cgttctgcgt accactggtg aacacgaagt gagcttccag
    14401 gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata attcgttatt
    14461 caacgagacg taaaaacgcc gaccattggt cggcgttttg ctttctattt ttcgtcaggc
    14521 attaatttcg caagtagatc cattcaccat tctcttgtgt ccataagtca ggatggtttg
    14581 ctggcgttag atgcatacga tcactcctta tgtctcggtc ctgcggcact cgctcgaaac
    14641 ctaaacccga gtagattgcg gctgaatttg ataaggaacg tagaccaatt ttcaaatcat
    14701 ggggtaaatc agaatacgcg tttagcaatg cagcaatgat gaatccgagg gcattagaaa
    14761 cgcctgtaac agtgcgaagt tggtcgatat aaagatcgat tgttggtagt tcagatgacc
    14821 agcaattagg catttgggca ggaagggtta caacgtatcc tatcgtttgg ccggtatagt
    14881 ctggagtact ataaccaggt cccaaaacaa cctcgtgtat tgtgtattta ctgttgggtt
    14941 cgaaggattc gacatcttgc aataaatatt tcagttctgc ggttaacctc gcacccgcgt
    15001 aagctttatt gtatttctgg gcgtgaatat tatctaatgt agcttcatgg ttccatccaa
    15061 aagaattatc accattcatt gt
//
LOCUS       DADWTH010000095        14935 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.096, whole genome shotgun sequence.
ACCESSION   DADWTH010000095 DADWTH010000000
VERSION     DADWTH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.096"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            596..793
                     /locus_tag="JNY02_003109"
     CDS             596..793
                     /locus_tag="JNY02_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434562.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(743..1936)
                     /locus_tag="JNY02_003110"
     CDS             complement(743..1936)
                     /locus_tag="JNY02_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9434563.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2072..3796
                     /gene="iucA"
                     /locus_tag="JNY02_003111"
     CDS             2072..3796
                     /gene="iucA"
                     /locus_tag="JNY02_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBB9434564.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3797..4744
                     /gene="iucB"
                     /locus_tag="JNY02_003112"
     CDS             3797..4744
                     /gene="iucB"
                     /locus_tag="JNY02_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBB9434565.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4744..6486
                     /gene="iucC"
                     /locus_tag="JNY02_003113"
     CDS             4744..6486
                     /gene="iucC"
                     /locus_tag="JNY02_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBB9434566.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6483..7799
                     /gene="iucD"
                     /locus_tag="JNY02_003114"
     CDS             6483..7799
                     /gene="iucD"
                     /locus_tag="JNY02_003114"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBB9434567.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7805..10000
                     /gene="iutA"
                     /locus_tag="JNY02_003115"
     CDS             7805..10000
                     /gene="iutA"
                     /locus_tag="JNY02_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBB9434568.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10551..10694)
                     /locus_tag="JNY02_003116"
     CDS             complement(10551..10694)
                     /locus_tag="JNY02_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434569.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..14842
                     /gene="sat"
                     /locus_tag="JNY02_003117"
     CDS             10955..14842
                     /gene="sat"
                     /locus_tag="JNY02_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HBB9434570.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSRTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4025 a         3554 c         3708 g         3648 t
ORIGIN      
        1 gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg ctaataaaat
       61 catggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa gagttttagt
      121 tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag gagttggtcc
      181 caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca tcagtgagtc
      241 aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat taaaaaccag
      301 taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg tcagtgaaaa
      361 agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc gccaatgccc
      421 tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc tgtttgaacg
      481 cgcagcgcag actcccggaa taaaagctga ctggcttgtc agaatgaaat ttaataacag
      541 agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa gaatcatgga
      601 gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc agcaagccag
      661 aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc tgatttactc
      721 cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata aatacagaca
      781 caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt gcgacacctg
      841 ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg tgactggtag
      901 cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt tgataagcgg
      961 gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac aaccatagct
     1021 gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc aggagcaggg
     1081 ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc gcagctactg
     1141 cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc gtagcgaact
     1201 ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca gccagtaata
     1261 ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg cactccggtg
     1321 tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc actggggctg
     1381 ccatcagcag ccccagtggc gccagaggat gaatggccag cattgctgcc gcgcacagcg
     1441 gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg gccatgcgat
     1501 tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt gccgacacgg
     1561 tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct gttgttatgg
     1621 cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg ccatatcctc
     1681 ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt ccccaccacg
     1741 gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat acagacagac
     1801 tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc tgtccgatac
     1861 cgagaagccc ggcactgaac gcaagtggcc agcaggacag tggtttttgg ggcgtatctt
     1921 cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt atttgtaaaa
     1981 ttaatttatc tgacaataac atttattgtt gataatgaga atcattattg acataattgt
     2041 tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa aaccagccac
     2101 agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag actggaaact
     2161 gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat cgctccattt
     2221 cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg cacgtctggt
     2281 cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta ttattgataa
     2341 gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc agcgcgtgct
     2401 ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg ccgctctgcg
     2461 tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac acgctttcca
     2521 ccctgcgcct aagtctcatg aaccgtttaa ccggcgggag gctgaacgct acctgcctga
     2581 tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa tcgccggtga
     2641 aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga atgcgcctca
     2701 gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt ggcaggggga
     2761 atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga ttaaagactt
     2821 aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct actgtgccac
     2881 cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca tccgtactct
     2941 gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa ccgacggctg
     3001 gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg gctgggccgg
     3061 gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc gtgaaaatct
     3121 gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc aggccgcacc
     3181 tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc gcctcggtat
     3241 cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag tgctaaagcc
     3301 gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc aaaatattct
     3361 tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc agggcagcgc
     3421 ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg cggaaaatat
     3481 tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta actccacttt
     3541 tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc tgatggctcg
     3601 tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct gtctcaacta
     3661 cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc tgaacgatca
     3721 taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta acccgctgca
     3781 ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg gactgcgctg
     3841 tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg cggtgttgca
     3901 ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga tatttatcgc
     3961 cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg agccacaggc
     4021 gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct tctggcagtt
     4081 accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga
     4141 gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt accgccgtta
     4201 cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga
     4261 acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc aaagtggctc
     4321 actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg cattcccgct
     4381 gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg cggcggaaga
     4441 ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc tgctggttgg
     4501 tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc tgacacatta
     4561 cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca ccgataacca
     4621 gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt tcgacttccc
     4681 acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg aggtcggtct
     4741 gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa aatgttgtct
     4801 gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca ctactgcatt
     4861 aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg ctggctgtgg
     4921 attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac gctgctgatg
     4981 cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat gcaggatttg
     5041 tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact gagtgccagt
     5101 gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca tcctaaattc
     5161 gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata tgcgccagag
     5221 tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat gatctggcgc
     5281 tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc gcaggagttt
     5341 acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct gccgctgccg
     5401 gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga ttttgccgaa
     5461 ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca gtcgctgcgt
     5521 accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct gaccatctac
     5581 aatacctcat gctaccgggg gatccctggc agatatatcg ctgccggacc actggcttca
     5641 cgctggctgc aacaggtttt tgcgaccgat gccaccctgg tgcaaagcgg cgcagtgatc
     5701 cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct tgcccgggct
     5761 ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg ccgctggctg
     5821 aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga aaacaatcag
     5881 ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg gcttacgcaa
     5941 ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg tgtcgcgctt
     6001 attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca gcgtgttctg
     6061 ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga aatggactct
     6121 ttgcctcagg aggtgcgtga tgtcacatcc cgtctgagtg cggactactt aatccatgat
     6181 ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat ggttcgtctt
     6241 ggcgtacctg aaaggcgatt ttatcaactg ctggcagaag tgttgagtga ttacatgaac
     6301 aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc acaaatcatt
     6361 cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg cagtcgcatg
     6421 ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa
     6481 tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat ctcagcattg
     6541 ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa catcctcatt
     6601 tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc tttctgaaag
     6661 atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat ctggtgaagc
     6721 acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt gaagagtttt
     6781 ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt cataccgttg
     6841 aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt cggggagaat
     6901 attttgccca caatatctgc cttggtacag gaaaacaacc ttatttacca ccctgtgtga
     6961 agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctga
     7021 gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg ttccttaatg
     7081 cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg cgtaataatt
     7141 ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa tatatttcag
     7201 gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag aaaatgacat
     7261 cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac caccgttttg
     7321 aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta acaactctgg
     7381 aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag ggcagagaga
     7441 gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg ccacaaatac
     7501 ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa gtgcgtgatg
     7561 acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc aacgccagca
     7621 tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga tctgcacgta
     7681 ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc gcactgattc
     7741 agtggcgcag cggcagccct gcttctttaa ctcactatac agcaaatatt caggaataac
     7801 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgctgtc
     7861 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     7921 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     7981 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     8041 tgtcagcagc caaagtcgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     8101 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     8161 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     8221 cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa ggtcagccgg aaaccatgat
     8281 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     8341 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     8401 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     8461 cggcctgcag cactctaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8521 atcccggcag cttcaactga taacgcagta ctataaaagt cagggagacg acaattacgg
     8581 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     8641 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8701 tgacagtgat ttcctgggac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     8761 gcggtactac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     8821 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     8881 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     8941 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     9001 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     9061 cattaatgat atttttaccg ttagcggtgg cgtacgctat cagtatactg agaacagggt
     9121 agatgatttc atcgactaca cgcagcaaca gaagattgct gccgggaagg cgatatctgc
     9181 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     9241 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     9301 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     9361 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     9421 tgaactgggc tggcgcttta tcggtgacaa cctgcggact caaatcgcgg catattactc
     9481 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     9541 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     9601 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     9661 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattaact gggcgccgga
     9721 accgtggagt ctgcgtgtac agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     9781 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggcagcttc cggtgggaac
     9841 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     9901 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     9961 gggccgaacc tttggtctga actactcagt gctgttctga cggggatgct tttgcaacaa
    10021 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
    10081 cagccttaac aagatattcc gtctccggaa tgggctgttc accactgaat aacattatgt
    10141 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
    10201 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctgag
    10261 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
    10321 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
    10381 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
    10441 attgttaata cgatggtgca cgatgcatcg tcagtgcccg gcccggagga tgccccgtag
    10501 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
    10561 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga
    10621 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
    10681 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
    10741 ccacattggg caaaaaaaaa caaaaaacga tcaattaacg atcaattaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa
    11701 tcttgaaata ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa
    11761 atgggtatta gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg
    11821 gataacaaaa tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat
    11881 aaatctgaat ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa
    11941 tgcagatacc actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa
    12001 tgggggaaat gttctgttta aggataattt ggattttggt agcggtggta ttatctttga
    12061 cgaaggccat gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga
    12121 tatcggaaaa gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca
    12181 caaaataggc cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg
    12241 tgaaggaaat gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg
    12301 aaatggtaag gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat
    12361 attttttact aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag
    12421 aattgccgcc actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt
    12481 tctggcaatc aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa
    12541 actaacgcac aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg
    12601 tagtgtcaac acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca
    12661 tgcaacagag catgcaatat tcagaagcag aacgagtcat tgctccctgg tatttctttg
    12721 tggaacggac tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa
    12781 ttctgattac aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg
    12841 ggtgtttaaa tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc
    12901 caatgttgaa ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc
    12961 ttacattgat aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac
    13021 tatcagtact aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa
    13081 cagccaaata gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa
    13141 taatactcct ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac
    13201 aacaaaaggt attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag
    13261 taaaactgta acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa
    13321 tgcaaatttc attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc
    13381 agcaaccatt actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca
    13441 ggcatgggca gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc
    13501 ccccaaagct acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat
    13561 taatcgtcta gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac
    13621 aacccttaca gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct
    13681 ggcccaagca gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca acctcctgtt
    13741 agtcgacttc attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc
    13801 accaaaagga actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga
    13861 tgtaacaccg gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg
    13921 ctataaatct gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg
    13981 ctataaagcc ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga
    14041 cattaacggt gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg
    14101 tggattcagt gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga
    14161 tggccttgac ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga
    14221 tgccttcagt ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga
    14281 gtccggagca tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc
    14341 aactttcgcc ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga
    14401 agtcggttac cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt
    14461 ttacggtgct gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat
    14521 gaaggataag gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt
    14581 ctccggtaag gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt
    14641 tgccaacggt gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa
    14701 agacggtcgt atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt
    14761 cggtcttgag tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc
    14821 caacttccgt tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg
    14881 gagagaatat gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DADWTH010000096        14927 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.097, whole genome shotgun sequence.
ACCESSION   DADWTH010000096 DADWTH010000000
VERSION     DADWTH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.097"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            83..364
                     /locus_tag="JNY02_003118"
     CDS             83..364
                     /locus_tag="JNY02_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434571.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="JNY02_003119"
     CDS             complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="JNY02_003119"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBB9434572.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="JNY02_003120"
     CDS             complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="JNY02_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBB9434573.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3461..4831)
                     /gene="purB"
                     /locus_tag="JNY02_003121"
     CDS             complement(3461..4831)
                     /gene="purB"
                     /locus_tag="JNY02_003121"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBB9434574.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="JNY02_003122"
     CDS             complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="JNY02_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBB9434575.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="JNY02_003123"
     CDS             complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="JNY02_003123"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBB9434576.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="JNY02_003124"
     CDS             complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="JNY02_003124"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBB9434577.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="JNY02_003125"
     CDS             complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="JNY02_003125"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBB9434578.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7968..9218
                     /gene="icd"
                     /locus_tag="JNY02_003126"
     CDS             7968..9218
                     /gene="icd"
                     /locus_tag="JNY02_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBB9434579.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9321..9644)
                     /gene="iraM"
                     /locus_tag="JNY02_003127"
     CDS             complement(9321..9644)
                     /gene="iraM"
                     /locus_tag="JNY02_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBB9434580.1"
                     /translation="MKWAVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9897..9941)
                     /locus_tag="JNY02_003128"
     CDS             complement(9897..9941)
                     /locus_tag="JNY02_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434581.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(10177..10287)
                     /locus_tag="JNY02_003129"
     CDS             complement(10177..10287)
                     /locus_tag="JNY02_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434582.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10340..10744)
                     /locus_tag="JNY02_003130"
     CDS             complement(10340..10744)
                     /locus_tag="JNY02_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBB9434583.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10965..11696)
                     /gene="bluR"
                     /locus_tag="JNY02_003131"
     CDS             complement(10965..11696)
                     /gene="bluR"
                     /locus_tag="JNY02_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBB9434584.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11901..13112)
                     /gene="bluF"
                     /locus_tag="JNY02_003132"
     CDS             complement(11901..13112)
                     /gene="bluF"
                     /locus_tag="JNY02_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBB9434585.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13426..13662
                     /locus_tag="JNY02_003133"
     CDS             13426..13662
                     /locus_tag="JNY02_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HBB9434586.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13705..13977
                     /locus_tag="JNY02_003134"
     CDS             13705..13977
                     /locus_tag="JNY02_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HBB9434587.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKLAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14006..14272
                     /gene="ariR"
                     /locus_tag="JNY02_003135"
     CDS             14006..14272
                     /gene="ariR"
                     /locus_tag="JNY02_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HBB9434588.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14385..14633
                     /locus_tag="JNY02_003136"
     CDS             14385..14633
                     /locus_tag="JNY02_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434589.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4004 a         3402 c         3476 g         4045 t
ORIGIN      
        1 catgaggatt ttgcttttga tataagcgac agccaccgcc agaaactgga agaatttagc
       61 ggggtgtaat gaggtcagca gcatgacaga agccgaaata acgggattaa tccgccgcgt
      121 tgccggaatc agccagcagg ctgacgaaca ggccacgcag ccggacagcg tgacagccga
      181 aaactatgcg cgtgttgttg ctgaggtgat gcgccgtgat ggtatagaac ttaatggcgt
      241 ggatatgcgc aacatacgaa ccagagttct tgagttgctg gcctaccgtc gccgcgtgga
      301 gatgtatcgg gagaaggaga aaatcacgta ccactggaag aagccggagc ggttacggcg
      361 gtaactcact gagattaaag caaagcgcaa aattgcgctg gctacatccc tcacatctga
      421 ggactgttaa ccagcaggcc ggaatatatt cagaaactac gaaattttcg cagtttaatt
      481 actcgtcagc ttaatcgcta acccgctctg atattttcgg aaacctcaat ttgaggaagt
      541 cggcgaggta actgtttgat attcccgata gcgcaaaaat gcgcggtcgg gcagaatgac
      601 caccacctca atcgtgacgg agagtgacaa ccagtgacac accgtgacaa aaagacggtt
      661 taaccgttca ttaaaaatcg acgtgatttt gtgctttccg gatgacgtgg ccaacgtcat
      721 tttttagcga aaaattctga ttaaggtcac tggctgaacg gtcataatga ccacgcagac
      781 catcgaggca accatagcga tcatcgttac ggttggagct tttccgccat tggtggaaaa
      841 ggtgcacatc tggatcatca ttacggtgat caagggtatg taaaacccac cagcctgatt
      901 aaaggttaac cggaaaaaaa atccggtagc cccgttatgg ggatccccat atcgacatta
      961 acgcccctca tgaattggtg cgcttccccc tggaaagatt acccgcctgt atatttcttg
     1021 tgtctatttg ttccacgttg tttcatacag tgcaccgaac ggtgtagtta atggtgtagt
     1081 caatttgcga tttttggcac tttttcaatg aaaattgttt aatcgaatca atgacttgat
     1141 gtagtggtaa aaggacatat ttattcatct ttcggcgcag aatgctggcg accaaaaatc
     1201 acctccatcc gcgcaccgcc cagcatgctc tctccggcga cgattttacc ctcatattgc
     1261 tcggtgattt cgcgggctac cgccagccct acaccttgcc cagggcgtaa agtatcaacc
     1321 cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg gccatcatcc
     1381 tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac aaactcgagg
     1441 caatatttac aggcattatc cagcacgttg cccatcacct cgacaaaatc gttctgctca
     1501 ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt gcgttgatac
     1561 actttgttca gcgctgaggt gagattgtcc agcagtgggg cgaccggatg cagctcgcgg
     1621 ctgagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat ttgctgtgaa
     1681 atgcggctga tttgctccag cattaccggc tcagcatcac tgacgctcat cttttcacta
     1741 cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact atgggtcagg
     1801 tcggtgagcg tcgtacggta tttgtcgtaa cgttcgcgtt cactttttaa caatcggttc
     1861 aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa ttcgcggtta
     1921 tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa actccaccag
     1981 gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac atagataaac
     2041 cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac caccacaatg
     2101 gttaattttg gcatccgcga tgttgccggg tagacgttta ccgccaccga gtgggtcatc
     2161 tctgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga atgatctcca
     2221 ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc attcgatttc
     2281 agccagtcag gctggatcat tttcatcagc cagggcacgt cacgttgcgc ccataaaagc
     2341 tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat attttcgggt
     2401 aactcgacat gcaatttatt gttttcccac tgcgcaaggg tatagaacag attgctctcg
     2461 ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat cagcgcgacc
     2521 attccgtagg caagcgaaag caccagtact accgctgccg ttgccaacag aaaacgtacc
     2581 cgcagcgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat tcgaacaggt
     2641 agccctggcc gcgaacggtg gtaatgactt cttgcggata ttgtgcctga atttttttgc
     2701 gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca tccggataga
     2761 gttgtagcat taacgaatct ttgctgacca ctttgccatt attgcgtatc aacgtttcca
     2821 taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat aattcacggc
     2881 gagagagatc aacctgaaac ggggggagcg aaatgacctg tgaagccaga ccgctattac
     2941 gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt ttagtcacgt
     3001 aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca cgggcggtta
     3061 ataccagaat cggcagtgaa acatcgttgc tacgccagcg gcgaatcagt gacagaccgt
     3121 cctcgtcagg caatccgaga tcgacaatcg caatatccgg taaatgttca ttgagataat
     3181 aatcggcttc tttggcatct tctgcgtcat cgacctgatg accagcatcc tgaatctgaa
     3241 ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc atttttattt
     3301 ctccctgtct taattattaa aatagtgtaa cgcgattatg tgtttatggg ggtaaacatt
     3361 aaataaacca gcggggaggg gaggtaaagt gaaaaaagaa aaagtggata atcttaataa
     3421 gcaggccgga cagcatcgcc atccggcact gatttgaggc ttatttcagc tcatcaacca
     3481 tcgtgatagc tcgaccaata tagttagccg gcgtcatcgc tttcaggcgg gctttctctt
     3541 cttctggcaa cgccagacca tcgataaact gcttcatgcc ttcggcgtca acgcgcttac
     3601 cgcgagtaag ctctttcagc ttctcgtacg gtttttcgat gccatagcga cgcataactg
     3661 tctggattgg ttctgccagc acttcccagt tgtgatccag ctcatccagc agatggtcac
     3721 ggttcacttc cagtttgctc acgcctttca gggtggattg atacgcaatc agcgcataac
     3781 cgatacccac gccgaggtta cgcagcacgg tggagtcggt caggtcacgc tgccagcggg
     3841 aaaccggcag tttgcttgcc agatgctgca ataccgcgtt ggaaaggccc agattccctt
     3901 cggagttttc gaagtcgatc gggttaactt tatgcggcat ggtggaagaa ccaatctcac
     3961 cagcaatggt tttctgtttg aagtggttaa gggcgatata accccagacg tcacggtcaa
     4021 agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa cagttcggca atgtagtcgt
     4081 gcggttcaat ctgggtagtg tacgggttcc attgaatacc cagcgaggtg acgaactctt
     4141 cgctgaactg atgccagtca acttccgggt aagcggcgat gtgggcgtta tagttaccga
     4201 ccgcgccgtt gattttgccg aggatctcca cctggttaag ctggcggtgc tggcgctcca
     4261 tacggtaggc gacgtttgcc atctctttac cgatggttga cggcgtggct ggctgaccgt
     4321 gggtacgaga cagcagcggg atatcgcggt actgaacggc gagatcttta atgccatcaa
     4381 tcagttgacg ccagtacggc aggatcactt catcacgcgc ggttttcagc attaatgcgt
     4441 gggagaggtt attgatatct tccgaagtac aggcaaagtg gatgaattca gaaaccgcgt
     4501 gcagttccgg gatctccgcc actttttctt tcaggaaata ctcaaccgct ttaacgtcgt
     4561 ggttagtggt acgctcgatg gttttgatgc gtgcggcatc ttcttcgctg aaattggcga
     4621 caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc aggaacttcc ttgatcgctg
     4681 cgtgcgcggc cagtttttgc agccaacgta cttcaacttg tacacggaat ttcagcaaac
     4741 catattcgct gaaaatcccg cgcagcgcgc tgagtttatc gccgtagcgt ccatcgacag
     4801 gggaaacggc ggtcagtgag gataattcca tagatcacaa ctccggggtt aaatgagcaa
     4861 gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa catcagttgt agacgtccgc
     4921 cgccgacctg gtgccagagc acggcggcgc gaatgcctgc cagcagggtt gcgcgaactt
     4981 tcgcctgcac ttgtgggctt tgcagtacag caggggaacc ggtgacctga atgcgcgggc
     5041 caagcgggct gatcacatca acatagatag cagccatcgc gctcatcagc gtttcggact
     5101 gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat ccggttgccc agagtgtcga
     5161 gcgcgccttt cgctgaggag agtttgcgct caagcaccat caagctgagt gtgtagcggg
     5221 ttaattcggc gtttaagccc tggcaactgc tggcattgag cacgccgagc agggtttcca
     5281 gcccgacgcg caggttggct tcgctaccgc caaaaaccgc cagcgtcgag ctggggttca
     5341 tatcaataat actgttgagt gagacgtgta gcgcatcggc atcacaatgc ccctggtgag
     5401 cgagttgttg caccaggcgt gccgactgac aaataccggc cagggcgagg gtgatgtcat
     5461 agtaattctt tgccacgttc actgcttcct tgttttaagt aaagataata atcagaccgg
     5521 cagcggcaga cgctgctcaa taataccgcc accgaggcac acttcaccgt tatagaagac
     5581 ggcagactgg cccggcgtca cggcggcaac cggctcatcg aaaatcactt taatgcgatc
     5641 gtcgtccagc gccttgacgg tgcacggaat gtcggtctgg cgatagcggg tttttaccgt
     5701 gcaacgcata gtgccggtga atggttcgcg atcgacccag tgcagctgct gggcaatcaa
     5761 cccgacagac atcagccgcg ggtgttcatg gccctgagcg acaaccagaa tgttgttttc
     5821 gacgtctttg tccaccacat accacggttc ttcggtgcct tctttggtgc caccgatacc
     5881 cagaccttta cgctgaccga gagtgtgata catcagcccc tggtgctcac caatttcatc
     5941 gccatcgacg gtaatgattt tgcccggttg cgccgggaga taacggccca ggaactcgcg
     6001 gaatttacgt tcgccaatga agcaaatgcc ggtagagtct tttttcttcg cggtgaccag
     6061 accaagatct tcagcaatct tacgcacctg cggtttttcc agttcgccga ccgggaacag
     6121 gctttgcgca atctgctcat ggctgagcgt ataaaggaag tagctctggt ctttattgct
     6181 gtccagacca cgcagcaggc ggctcttgcc atcgacatcg gcccgacgta cgtaatgacc
     6241 ggtagcgata taatcggcac ctaaatcttc ggcggcaaat tcgaggaagg ctttaaattt
     6301 gatctccttg ttgcacagaa tatccggatt cggcgtgcga ccggctttat attcggcaag
     6361 gaacagttcg aagacgttgt cccagtactc ggcagcaaag ttaacggtgt gcagttcaat
     6421 gccgagcttg tcgcagaccg cctgggcatc agccagatcc gctgccgctg tgcaatattc
     6481 ctcaccgtcg tcttcttccc agttcttcat aaacaggcct tcgacctgat atccctgttg
     6541 ttgcaacagc caagcagaaa cggaggaatc gacaccgccg gacatgccga cgattacttt
     6601 ttttgcggtt tcagacattg gatcactcac gacattgaac ttcaaggcgg cgtattctat
     6661 cacgcagcct tttagatgac accctttgta aaaggccagt taaaatcgcc aatcatctcc
     6721 agcggataac gttgcccgct ttgataacaa cgaatacttt ccgccaccag cggcgaacga
     6781 agatttgacg cctgtaaaat ttcttcggcg ctgacccaac ggcagcagtc gatatcgctg
     6841 tcatgaggct gggtcgggca tatttgctca agctcaatgg caaagaggaa acgcaaaaac
     6901 ggcgttttat ctggcgcaat ccactgatgc atacgaataa agtgttgcgg ctgcgcgctg
     6961 atgccggttt cttcccacag ttcacgggcg gcggcttcca ctaaggtttc atcggcttcc
     7021 agatgcccgg caggttggtt ccataacgct ttaccattaa tcgtctcttc aacgactaaa
     7081 aatttgcctt ctgcgtgcac cacgcaagca acggtaacgt gcggtttaaa cattttgcat
     7141 cctcaatctg tcacttctcg ccattcgcca ttggcaagat tatccaaaga gtaatctccc
     7201 atcgcatagc gaatcagtcg cagcgtgggg aaaccaacat gggcggtcat gcggcgcacc
     7261 tggcgattac gtccttcata taaggtgatc ttcagccagc tggtgggaat acttttgcgt
     7321 tcacgaattg gtggattccg cggccataac cacgcgggtt cgtcaaccag ctccgcgcct
     7381 gcgggcaggg tagggccatc atttaaggtt acgccattgc gcaaggcttc aagtgcgtct
     7441 tgtgtgggaa taccttccac ctgcacataa tagatttttc cggtgcgttt acccggctgg
     7501 gttaaacgcg cctgcaacgc gccgttattg gtcagcacca gcaacccttc gctatcgcgg
     7561 tcaaggcgac ctgctgcata aacgccctga accgggatga attcttttaa tgttttgcgt
     7621 ccggcttcat cggtgaactg cggaagaaca tcgtagggtt tattgaagag gatcacacgc
     7681 gtgggctggt tttcatgttt acgcctggta gaacgttgcg agctgaatcg cttaacctgg
     7741 tgatttctaa aagaagtttt ttgcatggta ttttcagaga ttatgaattg ccgcattata
     7801 gcctaataac gcgcatcttt catgacggca aacaataggg tagtattgac aagtcaatta
     7861 caaatcatta acaaaaaatt gctctaaagc atccgtatcg caggacgcaa acgcatatgc
     7921 aacgtggtgg cagacgagca aaccagtagc gctcgaagga gaggtgaatg gaaagtaaag
     7981 tagttgttcc ggcacaaggc aagaagatca ccctgcaaaa cggcaaactc aacgttcctg
     8041 aaaatccgat tatcccttac attgaaggtg atggaatcgg tgtagatgta accccagcca
     8101 tgctgaaagt ggtcgacgct gcagtcgaga aagcctataa aggcgagcgt aaaatctcct
     8161 ggatggaaat ttacaccggt gaaaaatcca cacaggttta tggtcaggat gtctggctgc
     8221 ctgctgaaac ccttgatctg attcgtgaat atcgcgttgc cattaaaggc ccgctgacca
     8281 ctccggttgg tggcggtatt cgttctctca acgttgccct gcgtcaggaa ctggatctct
     8341 acatctgcct gcgtccggta cgttactatc agggcactcc aagcccggtt aaacaccctg
     8401 aactgaccga tatggttatc ttccgtgaaa actcggaaga catttatgcg ggtatcgaat
     8461 ggaaagctga ctctgccgac gccgagaaag tgattaaatt cctgcgtgaa gagatgggcg
     8521 tgaagaaaat tcgcttcccg gaacattgcg gtatcggtat taagccgtgt tctgaagaag
     8581 gcaccaaacg tctggttcgt gcagcgatcg aatacgcaat tgctaacgat cgtgactctg
     8641 tgaccctggt gcacaaaggc aacatcatga agttcaccga aggcgcgttt aaagactggg
     8701 gctaccagct ggcgcgtgaa gaatttggcg gtgaactgat cgacggcggc ccgtggctga
     8761 aagttaaaaa cccgaatacc ggcaaagaga tcgtcataaa agacgtgatt gctgatgcat
     8821 tcctgcaaca gatcctgctg cgtccggctg aatatgatgt tatcgcctgt atgaacctga
     8881 acggtgacta catttctgac gctctggcag cgcaggttgg cggtatcggt atcgcccctg
     8941 gagcaaacat cggtgacgaa tgcgccctgt ttgaagccac ccacggtact gcgccgaaat
     9001 acgccggtca ggacaaagta aaccctggct ctattattct ctccgctgag atgatgttac
     9061 gccatatggg ctggactgaa gccgcagacc tgattgttaa aggtatggaa ggcgcgatca
     9121 acgccaagac cgtaacctat gacttcgagc gtctgatgga aggcgctaaa ctgctgaaat
     9181 gttcagagtt tggtgatgcg atcatcaaga atatgtaatc actacatgtg ttaaatattt
     9241 aaacgggcgt ataacacgcc cgttgtttta tttatgtgga tattattaat agcatatcga
     9301 gcatatttat ataaaaccta ttacttgagc ccatatgggc atatttttat aatacaactc
     9361 ttatgcaaac atttatttgt tatttcgctt tctcctggag gacactcttg actgcttttg
     9421 agtaaactcc ataaattctt gttgaatggt gcaatgttat aaatagtaat aggatattct
     9481 ttttccttaa ggataatacc aagcttaacc ggtgtaaata tccttccagg aggaagaaat
     9541 atagtagatt gataccagat gatcattttc atatcacccc atatggctga aaaagatatg
     9601 ccgcatgtag gttgaattac cgtgtcaatt actgcccact tcatttgtta tgtcttatcc
     9661 cacggtattt aatatagttc atttggatgt tcatttcttt attttgcata tgagtatatt
     9721 accccttcaa aaaataaatt aattaaaacg attgcttata taaaacaaaa tttaaatcaa
     9781 ggaatctcaa tggatgttaa acaaaatgag atttagtgaa aacaataaat tattcacctc
     9841 gttttagatt tgtttagcta taatgttata cattcaaatg aatgaacatc ctgtaattaa
     9901 aacatagcct ttatgctact tggtgccaat ttgctaaaca ttatggttgc ctttttatat
     9961 aacgataata atgaatataa acatgacatg agaataaggt ttcaattttt gagttatata
    10021 ggaaaggttt aacctgttcc tggctaaaat acatataact ggatgatgac taaaccaaaa
    10081 cacatatgcg ttaagtattg aaacgggcgt atggcacacc cgttgttttt ataaatatat
    10141 taaccgttat aaaataacgt ataaaaagtc aagtgatcac atttcaaata tcaatttgat
    10201 agtatgggct tggtgattat ttatgggtag caataaaagg acagtattta tcatccatag
    10261 ggatagtctc tgtactttta ttcccattat gctaatgcct tactgaatta tgaagcattt
    10321 cttaagcatc caactttagc tagattaatg gtttattatt ttctacatct tcaatatata
    10381 aaagcgtatt atcaatggcg tagtaactgc gtttgttatg attaacatca gtaacccaac
    10441 ggaaaacgcc cgcgcctgct agtgttgaac agtattcccg aaatgtagat tttccgcaaa
    10501 tatgaagcaa tgcggcctct tttattttag caggattctt ggtcgtacta acttttaact
    10561 ggttcctggt tcctcttaat aacaaaaccg tgtcatcgtg agtaataatt ctgatgttat
    10621 ccgtagccag ataataaatg taatgtgcaa tacggtgatg ttttaattct gaataaaacc
    10681 aggagaagtt ttgctctttt ctcacttgct caaacatctt ttgaaaaaca acgacctgat
    10741 ccatcaggat aataacctct tgttagttgt gagactgcgt agtgtgcacg atcggtttta
    10801 ccacttcaat ctggtctgtc ctttggctgt gatatgtaca gagtgtgata gagggattac
    10861 ctgaattctc ccggtgagca ttttgcaacg gaccagctcc ggtacaaatg ctgttgtggg
    10921 ttcagattat aacattctgt ctaaggggcg ggataaaggt gaaattaggg ggcatgaaag
    10981 atgactttat aaccttgctc accccaatgt tgtaaaagtt cgttttgcct tctcgttggt
    11041 gccatgcctg tccagacaat caatgtttgc gtcgggaaca gttccgggcg cggcgattca
    11101 atgggatcag caagaacaga aatgtgccat cctgacaatg ataaacgcca tgcttccagc
    11161 cacaatcgtg ctctctcttc aacattccac gccatcagaa tggcttcttt accgggctta
    11221 cggcgcgttt cgaaaagcga ggttgctgcg tattcaatta atgcgccgtc aaacatactg
    11281 ctcataatgc gggaggtgtt gtgatcaagc acgagacgct ggcgaacagg aaggtaaaca
    11341 tgattaatca attgatctac tgggtactct cgacccagtg aaataattct cgcgcgtagt
    11401 ttggcaggat tagccatacg aagaattgac atcatctctt cttgcaggcg gctccagtca
    11461 tcttccgtat cctggctggt ggtttccagt aatgctttaa ctttgcctac agggacgcca
    11521 ttactgatcc aacgcttgat ctcttcgatg cgttgtatgt cttcttcatc aaagagtcgg
    11581 tgtccgcctt cactgcgctg tggttttaac aaaccgtagt ggcgttgcca ggcccggaga
    11641 gtgacaggat taatcccgca acgttcagca acatcaccaa tgctgtaata agccacaatt
    11701 cctccttgcg gtcacaaatc tccgtcgcct gtacacgacc caataatact ttgtacaata
    11761 tacgctaaaa ttgtacaaag tataaataag atttcagcta aattggatga aacattattt
    11821 ttaatgtaga ttaaatttaa acataacgta ttcattttca cgatgattta ctgaaatcat
    11881 gtgaaagaat gtgctgaaaa ttattttttc tctggccacg ctatggaagg gataccattc
    11941 aatttagctt tagcaaacag atctccctga aacatctcaa ttcctgcgga ttcaagccac
    12001 atccactctt ctggtgttgc cacgcccata gcactgactt gaatttcaag tgatgtacag
    12061 cattttatga tcgcttgaat aattgcctgc cgtggcccgc ttttatgaac attggtaatc
    12121 aattcctgac tgattttaat tctgtcaggc tggaagcgtg acaggagtaa caaaccggca
    12181 aaacctgcgc caaaatgatc aattgctaca ctgataccgg cagccttaag cgatttaatc
    12241 gcttctgcaa actcatcaaa ccgagatata acttcacttt cagtaaattc aacgatgatt
    12301 tgttcaggca ccagagcatt ggcctttatt tcatcaagta aaaaagagac tgcgtcaggt
    12361 tcgttaacca gggtcatagg taatagattg attgaaatca ttttatcacc gagctcaagt
    12421 gcgtgtgtca tcgcgaatgc aagcgcctta cttttgagat ccgctgtgta gatttccccg
    12481 tctttacgct gcccaaccgc tatggctgat gggctatctt catttttttg cacaatggct
    12541 tcaaaagcga ttatccgccg cgataaggga tcgacaatag gatgaaaggc aaagtggtcg
    12601 tttatagttg gtgaaagggt acaggaatca agttctttat cagatccgtc agcgataaaa
    12661 agccaggagt cttcggcagg gatctcgaaa taggttgatt gttcggttgc aaggacaaaa
    12721 gtacgaaaaa attgtagcgc tctgtcatca taagttagct gaaattttga tgtgcctttg
    12781 tcgaatacgg cctgtaaaac gtcatctcgc tcgtgcaggc gcaaatcaaa taattccatt
    12841 cccgctttgc caaaacggcg agcaggcgcg taatcgcaca gcagctcaac aatattatag
    12901 tgccgtggat cttggcatat agcccgatat atcattttaa cctgttcttc cggaccttcc
    12961 agaagctgga aaaaatgaga accattaaac agtaagatcc ctgttacgtc agactgcatg
    13021 ttcctgcgat ttgctatcga aaccatttct tcgatttttt tgacaggttc gtcgtcacgt
    13081 atatggctac gataaataag ggtggtaagc attaacaatc cagggtaatg ggtgaggcga
    13141 gagtaagacg gtaacagaca tatcttcttg tgtctttctt ttaataccaa aacataaccg
    13201 tttctttaca ttgataaaaa atggaaaaag ttgaacacga gttggcgaaa aatcttgtat
    13261 agattgtcag ttaaatgatg caatatgttt tatcataaca cattgtttta tatacattag
    13321 tactaattgc aaaaaattaa tttatcattc tgtacacata tttcgtacaa gtttgctatt
    13381 gttacctcac ttcacattga ttaacatttt taacagaggc gtagcatgca tcaaaattca
    13441 gtgacttcag attctgcggg agcaatcact cgttactttg caaaagctaa cttgcctact
    13501 cagcaggaaa ctctcgggga aattgtgact gagattttga aagatggacg taatctgagt
    13561 cgaaagtcgc attgtgccaa acttctttgc cgactggaac aagcgacagg ggaagaggaa
    13621 cagaaacatt ataatgcact aattgggttg ctttttgaat aatgcaacag cctgattatt
    13681 taacaggtta gctatgaagt cgttatgaag acatctgata atgaacgtat aaaatatgaa
    13741 attactggcc aggcggtgct ccagatactg cgcatgaaga taaatttttc attgcagact
    13801 cttattaagc aattactcgt aatgaaattg gctgaggaag atgctttccg acgcgattta
    13861 attgacagca taattcgtga ttttagcaat agtgattcag gagggccaaa ccgaagaaca
    13921 gcgacagctg acaataaaag tatgttcaat ggtaagaaaa taaacagaat acattaaaat
    13981 ttcataagta agatgagagg ttaccatgct tgaagatact acaattcata atgcaataac
    14041 tgataaagcg ttagcaagtt actttcgcag ttcgggtaat ttgttagaag aagagtcagc
    14101 agtgttaggg caggctgtca ccaatttaat gctttcaggc gataatgtta ataataaaaa
    14161 tattatctta agtctgatac actccctgga aacaacaagt gatattctca aagctgatgt
    14221 gattagaaaa acactggaaa tcgtgttgcg atacacagct gatgatatgt aacttctaat
    14281 tattaagtat aagtttatag aaaactcatt catcttattt ttgtctgtcg ctttagacta
    14341 tacacagcat aattttattg ggttaatatt tctacgaggc tgacatgaat aattcaatcc
    14401 cagagagatt tatttttcaa tgtgctttgt ttaaaaatct cgagagggag gtgttcatga
    14461 cgcatgggta tgttgatagt catattattg atcaggcttt acgcttgcgt ttaaaagatg
    14521 aaaccagtgt gattctttct gatctctatc tgcaaatatt gcagtacatt gaaatgcata
    14581 agactacact aacggatatc attattaatg acagggaatc cgtgctctct tagaacttga
    14641 gtaaggtttt agaataaaca tcagctgtat caccatgctg atgcaaagtg agcatttcag
    14701 gcgttatgct ttcttattat gtccgcaata tcaggtgtca agaatggaga gttctcgctc
    14761 tccattcttg acgcctgata tcccgcctaa cttattttgt actgcctaca ttatgctcaa
    14821 taattaatgg gttagatcaa gatggagaaa attttatctt cggcgtggat atttcttatg
    14881 ctctctacca tgcatctctg tagatgccag cgcgagcgaa tgaatgg
//
LOCUS       DADWTH010000097        14527 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.098, whole genome shotgun sequence.
ACCESSION   DADWTH010000097 DADWTH010000000
VERSION     DADWTH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.098"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(337..582)
                     /locus_tag="JNY02_003137"
     CDS             complement(337..582)
                     /locus_tag="JNY02_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434590.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="JNY02_003138"
     tRNA            complement(1227..1302)
                     /locus_tag="JNY02_003138"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="JNY02_003139"
     CDS             complement(1408..2115)
                     /locus_tag="JNY02_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBB9434591.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAIPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="JNY02_003140"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="JNY02_003140"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBB9434592.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADISPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="JNY02_003141"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="JNY02_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBB9434593.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JNY02_003142"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JNY02_003142"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBB9434594.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEVWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="JNY02_003143"
     CDS             complement(7299..7574)
                     /locus_tag="JNY02_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBB9434595.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JNY02_003144"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JNY02_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBB9434596.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="JNY02_003145"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="JNY02_003145"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBB9434597.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="JNY02_003146"
     CDS             9534..9860
                     /locus_tag="JNY02_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBB9434598.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="JNY02_003147"
     CDS             10044..10763
                     /locus_tag="JNY02_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBB9434599.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="JNY02_003148"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="JNY02_003148"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBB9434600.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13109
                     /locus_tag="JNY02_003149"
     CDS             12102..13109
                     /locus_tag="JNY02_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBB9434601.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13165..14466)
                     /locus_tag="JNY02_003150"
     CDS             complement(13165..14466)
                     /locus_tag="JNY02_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBB9434602.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKHRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTLPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3807 a         3477 c         3732 g         3511 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct gcttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggtattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggcgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatatctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccagac ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcagtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttatcctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc
    13861 acggtgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14341 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14401 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14461 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
    14521 cacaagg
//
LOCUS       DADWTH010000098        14504 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.099, whole genome shotgun sequence.
ACCESSION   DADWTH010000098 DADWTH010000000
VERSION     DADWTH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.099"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="JNY02_003151"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="JNY02_003151"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBB9434603.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="JNY02_003152"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="JNY02_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBB9434604.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHEPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNY02_003153"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNY02_003153"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBB9434605.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /gene="fumB"
                     /locus_tag="JNY02_003154"
     CDS             complement(3965..5611)
                     /gene="fumB"
                     /locus_tag="JNY02_003154"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBB9434606.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="JNY02_003155"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="JNY02_003155"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBB9434607.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIKSDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="JNY02_003156"
     CDS             7086..8594
                     /locus_tag="JNY02_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBB9434608.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8639..9904)
                     /gene="uidC"
                     /locus_tag="JNY02_003157"
     CDS             complement(8639..9904)
                     /gene="uidC"
                     /locus_tag="JNY02_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBB9434609.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(9943..10110)
                     /locus_tag="JNY02_003158"
     CDS             complement(9943..10110)
                     /locus_tag="JNY02_003158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBB9434610.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10126..11316)
                     /gene="uidB"
                     /locus_tag="JNY02_003159"
     CDS             complement(10126..11316)
                     /gene="uidB"
                     /locus_tag="JNY02_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBB9434611.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(11313..13124)
                     /gene="uidA"
                     /locus_tag="JNY02_003160"
     CDS             complement(11313..13124)
                     /gene="uidA"
                     /locus_tag="JNY02_003160"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBB9434612.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13512..14102)
                     /gene="uidR"
                     /locus_tag="JNY02_003161"
     CDS             complement(13512..14102)
                     /gene="uidR"
                     /locus_tag="JNY02_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBB9434613.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14331..>14504)
                     /locus_tag="JNY02_003162"
     CDS             complement(14331..>14504)
                     /locus_tag="JNY02_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB9434614.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3637 a         3775 c         3459 g         3633 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaacccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattaagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagtgata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggaggtttt tctctccggc gttatttctt
     8641 acttcagcat aaagtcatac tgaatactca ttttcacatc ccagtaattc aggcgatcgc
     8701 ccagcgtact gccatcgcgc ttttcccgcc ctttatcggc agcaagaaag aaattcatcc
     8761 cttgtaacgg cccttcgctg acccgatagt tcataccacc taagataccc gcgccttcaa
     8821 tccgcacatc tcgtttactg gattcatagc cgcgagtcac aatcggtgca aatgtcagcg
     8881 taaattgcgg agttacacgg tagttgacac ctaattgcca ggactgcatt ccttcacggt
     8941 tacgatcaag acttaatgaa aagggatagc caatgtcggt atccaccacg gcaccattca
     9001 ttgcatggcg caaataacca ccactgccaa atacgcttcc ccacggcgca ttccaggtca
     9061 attgtccact aaccaacgcc gtttcattgg gctggctggt attacgactt aaaccttcga
     9121 gctgcgcgta aaagccgagc aactcccctt tcaacgtata attcgggtcg agcggtatgc
     9181 ttttctcgag aatactttgt atctgattgg aaacatcttt ctgtaccgat gcccccagag
     9241 aatagcgact ggtatcatca tgccaactgg cggctaaaaa agaacgtgcg cgatgtttat
     9301 cgtattttcc cgtctggctg ttatacaccc cttcatcacg aataccggtt gttgaatcag
     9361 tccataaaaa tactcgtgaa cgccacattc cccacagatc aatatttggt gttaaagccc
     9421 aataacctaa tgctccttca gaaactgtcg gtaatgtacg ttgcgaactg ccagccatta
     9481 acggaatacg ataaggtaag ctaccataac tataatccgt cccgaagcga cctaatttaa
     9541 gcaggctatt ttcactgggt tttattttta ctgcccccag ggcaaagcca aaacttttat
     9601 caccatcaag ataccaccgg gtactcatat cagcacgagc acctaattta taaacataat
     9661 acgctccgcc ttcaacgcca ataatattgg aataataacc actattgaaa tcgagcaatc
     9721 ctccctgtac ccatgcgtaa atatctcttc ccgcgccagc actgggctta tcagccctgc
     9781 gcaattcgtt tttcagtctg atgcgtagtc ccgcttcatc gtcagccagt tgcgccacgt
     9841 aagcagaggt aaggccagag gcagccgtca ggcaaataac ggccatggcc actatttttc
     9901 tcatagttgc acctttgatg ttctgattat ttttattgaa tattaattag tgatatcgct
     9961 gattaattgc tgctgcactt ttttacgatt atcaatttca accacgattt ctttgaattt
    10021 tttatccgtg agcggataaa accagataat aacgaatgcc agtagcataa atccgcaagg
    10081 tactaaggca attgatgtgc ggatgcccat aataacttcc ggcgtttaca cctgattggc
    10141 gatatatccg cttaatccca aaataaaggc aggaattgaa cctccgattg cctgaccgca
    10201 tttacgggta aatgagaata gtgaataggt gagcccttca attcgcacgc cggtcaggta
    10261 ttcaccgtat tctacggtat cagcttccag tgcccacatc acagtcatag taacgccctg
    10321 accaattgaa gcgatggcca acgcaaccaa cgccaccggc agtgaccaga cggaaaccca
    10381 gaagaatagc agataaccgc aggttcccag caaagcgcca atcaggaagg tattcttttt
    10441 accgatcctc gcgaccatcc ccggcacaag cggtgccgat gccacagtac caaccaggtt
    10501 ttgcaccagt accagcacag tgaacagccc ggtatcattt aacacatagc gcacgtagaa
    10561 caacgacgag gcgctgaccg cgaaggtcga aatcagcaca cacagcgcac cgatgcacaa
    10621 cataaacagc gggcgattcc gtttcagggt ttgcaaactg atcttcaatg acggctgcgc
    10681 cacgatacgt accacatttt cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc
    10741 ggcaatcgcc agcacaatcg tccagaaatg gtataccgac accatctctt ccgggctgga
    10801 gttcttaatg ctcggtccta tcagaaatgc caggcataca aaggtcaatg aagcggcaat
    10861 cccacgagcc gcgcccagac gggcgcgaga ttgtggttgt tgggtcatcg cggtagcaag
    10921 tgaaccataa ggaatattca ccaggctgta gcaaagcccg aggcccatgt aggtcaaata
    10981 tgcatacacc actttgctac catggctcca gtcggtcggt acccagaata ccagcacgct
    11041 gaagatcatt aacggcgcag taccgaagag taaaaacggg cggaattttc cccagcgggt
    11101 attcacactg tccaccactc gtccggcaaa gacgtcggcg aaggcatcga atacccgcac
    11161 cagtaacagc atggtgcctg ccgcagcggc accgacgcca gcgacgtcgg tgtagtaact
    11221 caataggaag agcgccccca ttgcgaaggc gaagttattg gcgacgtcac cgaggctgta
    11281 gccgacgatg gtgcgccagg agagttgttg attcattgtt tgcctccctg ctgcggtttt
    11341 tcaccgaagt tcatgccagt ccagcgtttt tgcagcagaa aagccgccga cttcggtttg
    11401 cggtcgcgag tgaagatccc tttcttgtta ccgccaacgc gcaatatgcc ttgcgaggtc
    11461 gcaaaatcgg cgaaattcca tacctgttca ccgacgacgg cgctgacgcg atcaaagacg
    11521 cggtgataca tatccagcca tgcacactga tactcttcac tccacatgtc ggtgtacatt
    11581 gagtgcaggc cggctaacgt atccacgccg tattcggtga tgataatcgg ctgatgcagt
    11641 ttctcctgcc aggccagcag ttctttttcc agtaccttct ctgccgtttc caaatcgccg
    11701 ctttggacat accaaccgta ataacggttc aggcacagca catcaaagag atcgctgatg
    11761 gtatcggtgt gagcgtcgca gaacattaca ttgacgcagg tgatcggacg cgtcgggtcg
    11821 agtttacgcg ttgcttccgc cagtggcgcg aaatattccc gtgcaccttg cggacgggta
    11881 tccggttcgt tggcaatact ccacatcacc acgcttgggt ggtttttgtc acgcgctatc
    11941 agctctttaa tcgcctgtaa gtgcgcctgc tgagtttccc cgttgacagc ctcttcgctg
    12001 tacagttctt tcggcttgtt gcccgcttcg aaaccaatgc ctaaagagag gttaaagccg
    12061 acagctgcag tttcatcaat caccacgatg ccatgttcat ctgcccagtc gagcatctct
    12121 tcagcgtaag ggtaatgcga ggtacggtag gagttggccc caatccagtc cattaatgcg
    12181 tgatcgtgca ccatcagcac gttatcgaat cctttgcctc gcaaatccgc atcttcatga
    12241 cgaccaaagc cagtaaagta gaacggtttg tggttaatca ggaactgttc gcccttcact
    12301 gccactgacc ggatgccgac gcgcagcggg tagatatcac actctgtctg gcttttggct
    12361 gtgacgcaca gttcatagag ataaccttca cccggttgcc agaggtgcgg attcaccact
    12421 tgcaaagtcc cgctggtgcc ttgtccagtt gcaaccacct gttgatccgc atcacgcagt
    12481 tcaacgctga tatcaccatt ggccaccacc tgccagtcaa cagacgcgtg gttacagtct
    12541 tgcgcgacat gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagagc
    12601 attacgctgc gatggatccc ggcatagtta aagaaaccat ggaagtaaga ctgctttttc
    12661 ttgccgtttt cgtcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg
    12721 ttcacacaaa cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct
    12781 tcaaatggcg tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg
    12841 ccgtaatgag tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt
    12901 ataaagactt cgcgctgata ccagacgttg cccacataat tacgaatatc tgcatcggcg
    12961 aactgatcgt taaaactgcc tggcactgca attgcccggc tttcttgtaa cgcgctttcc
    13021 caccaacgct gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg
    13081 agttttttga tttcacgggt tggggtttct acaggacgta acataaggga ctcctcatta
    13141 agataaaaat actggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag
    13201 ttatctgtga gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa
    13261 aaaaccaaaa actgcaatac ccacagcgtg atatggatcg cattaatctt taaaacagcg
    13321 ttgacagaag ccataaaaca agcaatcgag cgttcgttcg agagtattgg gaacgataaa
    13381 ctctcatgac atatggctac ggtgaatatt ttggggggga attctccgaa cacggggagg
    13441 ataatttttg aacagagaaa ttacgatatg aaacgcgggc acagttatca ccagcccgcg
    13501 aaatgagagg atcaggatgc ggttaatata ccgccaatca tcgcgcgtaa tccctgagca
    13561 aacgacgctt cacgcgccag gtcttccgct tcgatatcgg ccagtacacc gtaggtcaga
    13621 tccagcagta aacgtgaagt cattgccgtg ttgatgtctg ggcttatctc gcctttttgc
    13681 tgggcatcac gcatccgctg ggcaacaaat tcagtgatcg tcatatgctt atttttaagc
    13741 atggcggcga cctgtgggtt acgcatccct tccgccataa tttcgaccac cagcgcccgt
    13801 tgcccaaagg cttcatgggt gagagagaca atagactcga ccatatagtc aacaaaatga
    13861 atcccttcaa tcggttcccg gaaacgagcc agcgccctct cctggtcctg taagataatc
    13921 gcctgaatca aggcttcttt ggagatgaaa tggtgataga gcgtcccagg actaatggcg
    13981 caagatttac agatggcttt catcgaggca ctgtgaaatc cattttctga aaaaatctct
    14041 ctggcagcat tgaggatccg ggtccgtgtc ggttgtgctt cagtctgcat gttatccatc
    14101 attaaaccat tatagttagc gtgaataact agtttaccgc gttgtcaggt gagcgcctat
    14161 cattgctgtt gaatagtgcg aaggtacact ctattcgtat acttttcacc cagcaattgg
    14221 ttaaccaatt gccgtgattt ggctgccagt tgtttagcag agacgttgat gaattgcaca
    14281 cgctatcccg gtaaacaaca catacgaccg tttaccggtt cgttagtgta ttaattgagc
    14341 tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct ggctgcaggc
    14401 gagcaaagga acagcgccgc gttagcaata tcttgcggtt ggcccagacg tctgatcggc
    14461 gtgtgctgta acattttttg ttcaatttct gggtaatgac tcca
//
LOCUS       DADWTH010000099        14162 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.100, whole genome shotgun sequence.
ACCESSION   DADWTH010000099 DADWTH010000000
VERSION     DADWTH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.100"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNY02_003163"
     CDS             complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNY02_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBB9434615.1"
                     /translation="MSTSIRICSYLLLLLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSL"
     gene            complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNY02_003164"
     CDS             complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNY02_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBB9434616.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKHQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNY02_003165"
     CDS             complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNY02_003165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBB9434617.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNY02_003166"
                     /pseudo
     CDS             complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNY02_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNY02_003167"
     CDS             complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNY02_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBB9434618.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPSVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNY02_003168"
     CDS             complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNY02_003168"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBB9434619.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6140..7279)
                     /gene="wza"
                     /locus_tag="JNY02_003169"
     CDS             complement(6140..7279)
                     /gene="wza"
                     /locus_tag="JNY02_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBB9434620.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLIPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7588..7737
                     /locus_tag="JNY02_003170"
     CDS             7588..7737
                     /locus_tag="JNY02_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434621.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            7938..9521
                     /locus_tag="JNY02_003171"
     CDS             7938..9521
                     /locus_tag="JNY02_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBB9434622.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNY02_003172"
     CDS             complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNY02_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBB9434623.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNY02_003173"
     CDS             complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNY02_003173"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBB9434624.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNY02_003174"
     CDS             complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNY02_003174"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBB9434625.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13480..13899
                     /locus_tag="JNY02_003175"
                     /pseudo
     CDS             13480..13899
                     /locus_tag="JNY02_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(13924..>14162)
                     /locus_tag="JNY02_003176"
     CDS             complement(13924..>14162)
                     /locus_tag="JNY02_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB9434626.1"
                     /translation="GSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQ
                     GKRHNQVLIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT         3281 a         3739 c         3593 g         3549 t
ORIGIN      
        1 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
       61 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
      121 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
      181 taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc actccagcaa
      241 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
      301 aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
      361 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
      421 gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
      481 cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
      541 caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt gtggcgaaat
      601 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
      661 agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt taaacgccgt
      721 gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc gttctaaaaa
      781 cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg
      841 ggcgattttg acgttaacca gcaggtagat cagtagcagc agcaggtagc tacagattct
      901 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cgactgcgtt
      961 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
     1021 gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt tgcagcactt
     1081 cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
     1141 aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc tgattgagcg
     1201 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
     1261 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
     1321 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
     1381 tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct tcggttgcca
     1441 tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt
     1501 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcgcggaata
     1561 actggtgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat
     1621 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
     1681 agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca tccggtttat
     1741 ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag tagctgtgca
     1801 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
     1861 tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg
     1921 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgaca ctctctttgc
     1981 cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     2041 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     2101 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
     2161 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     2221 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     2281 atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
     2341 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
     2401 cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
     2461 cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
     2521 agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt tggcgcgcag
     2581 atcttccagc atctttagcg ccccgaatac catcagccag acgtttgccg ttacgcaccg
     2641 acagcagcgt caacaaggtg cgccaggtca tacgtttatt gcggatctgg tagggggtaa
     2701 acagctgata ttttttactg gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga
     2761 agtatccgga gaattttttc ggtgacgagg tgatctgcat ttcgccgtga ttgatatgca
     2821 ggatctgcgt cgcctcttcc actttccacg gttcgccgta ctccaccacc atccgcagga
     2881 atatgtcgta atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc
     2941 atgcccaggt aaagacctga ttgccaatga tattacgttt atagaacagg cggcgtgagt
     3001 acggcgattt gggatacagt ggtaagctcg ccggttggga ataaacttct ccctggcaaa
     3061 cgtagtcgtt agcgtacaaa aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga
     3121 cgctcaggcg gttgggcgtc cattcatcat catcatcgat cccggtgatg tattcccctt
     3181 gcgccagcat aatcgcctgg ttacgcaccg cgcacgcccc gctgttaatg tcgttgtgga
     3241 tgtaagtgat acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg
     3301 tggagcaatc atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt
     3361 ttatcgcccg aatcgccagt tgttggcggt tccaggttgg catatagatt gagatcagcg
     3421 gattgtcttt cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg
     3481 tgagcgcctt atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga
     3541 cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga
     3601 gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc
     3661 cacttctttc aatgtgttga ccgcataacg cgccaccatt aacgtggttc cgacatgacg
     3721 accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc
     3781 atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatta acagttcaga
     3841 aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt
     3901 agcagcggta gtaatatcgc cctgaccaat cagaatctcc gacagcccat tgacattatt
     3961 ggtgcccaac agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacgcg
     4021 tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt taccgattga
     4081 cgggctaacc ccggtcatca tcaacacatt gttctgcgcc tgcatcatcg cgaagtgcag
     4141 actggtacgc agactacgga tggcttcaat cgccagatcg gttggattcc ccactgccag
     4201 tagctggctc tgtttatagc gtttaacccc tttgatggtt ttgacgctat cgcgcgcttt
     4261 ctgccactcc gacagcggga tgctggcata gacgctgatt ccgtgttctt ccagcacttg
     4321 cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat
     4381 caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta gcacgctagg
     4441 ctgggtgatt gccgggtcaa taatgcgcac atcgccgacg gtgctggctt cggtgatttt
     4501 cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg
     4561 ggtcagacgg acaatttcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt
     4621 ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac
     4681 tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc
     4741 gatgttcacc atcgaatcga gcaccgcttt cgcttcaagc ggcagatcaa cagaatcttt
     4801 atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg
     4861 taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt
     4921 ttgttcctga tagttacgag cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc
     4981 accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct
     5041 gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc
     5101 cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc
     5161 cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt tgtcatccag
     5221 cacattaagc gtaaacacct gatccgccat ctctttcggg cggttaaagg tagtcacttt
     5281 caccgtctcg ttctgacgcc ccatcaggcg atcccagccc gccccgaaaa tcgggaacgt
     5341 gtttttgctc accgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg
     5401 cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgagcc
     5461 gatatcctgc accagcgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact
     5521 ataaatcggc gtggcgaaga aagtgtaaac cacggcacag agggcaaata cgacggtgat
     5581 gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc
     5641 atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc
     5701 tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct
     5761 gcaaacgttt cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga
     5821 ccaaacagca tcactttgcc gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg
     5881 cgcttatcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt
     5941 gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca
     6001 gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa
     6061 cgttgcagta agcgttccgc catcggggaa cggcaaatat tgcctacaca gacaactaag
     6121 atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac
     6181 ggacaccgct aatagttggc agcaactgat tgatcagacg gttccagcgg gaaaccgggg
     6241 cggtggtgac atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg
     6301 acgtcgcatc ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgccct
     6361 cgcctttcag cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac
     6421 cttcggcatt gcccagagct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc
     6481 tctgtttctt cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga
     6541 gaatatcgcc gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct
     6601 gtaaagaaat gcgttcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg
     6661 tgtcggtcag gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggt acgttggtga
     6721 tcgcctgttg accggattta ttcacctgac ctgagatata ggccttttgt gagcggaagg
     6781 cggcgatatt aacgtccacc tgcgggtcag cgatgtatgt cgctaagcgc ccggtaatat
     6841 cactgcggat ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa
     6901 acatagtgcc gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccag
     6961 ctggcgtggt gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga
     7021 cgcgatactg gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg
     7081 ggcgtgggcg taattggtca atcaggcgcg ggatcagcgg ataaacattc accattttgt
     7141 cgagatcgaa atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc
     7201 tgcccggaag tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt
     7261 tcattttgga tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact
     7321 attaatagca atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg
     7381 gcatcagtcc taaaaaaatt gatttcatca gcaggctatt gacagaataa ttcagactgg
     7441 tctttcaggc atccagacac gctaccgccc ctggcttttt agctaccaat acactgattg
     7501 agcttaattt ttcaaaccct ctctgcatac aatcgttgat gagaaagggt tattgcggaa
     7561 attaacttcc gaatataagg tgacattatg gtaattgaat attggctcgc caataatgca
     7621 ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt
     7681 tttaagaatt atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa
     7741 tatttaatat tgcgtctcca catgcgatat ttcttaaata atgttttatt attaccgctt
     7801 ttaattcagg gataatgtga ggttattatc ctcaaataat atgagataaa tagtgctgca
     7861 acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa
     7921 taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca
     7981 cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg
     8041 ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg ctgctggcga
     8101 tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg actcaaccgc
     8161 tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt
     8221 tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca
     8281 ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac
     8341 tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac
     8401 tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt
     8461 taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc ttcctgttga
     8521 tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt
     8581 acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct atctttaacc
     8641 ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa gcggtaatgc
     8701 gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg
     8761 tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac
     8821 ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata
     8881 tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc
     8941 ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg
     9001 acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc
     9061 cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc cgtaatgccc
     9121 gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa
     9181 gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag atcgacgccc
     9241 gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg
     9301 aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg
     9361 ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg
     9421 gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta
     9481 taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg ccattttacc
     9541 caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt aaacggacat
     9601 cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag gctggggcag
     9661 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgtacccg
     9721 atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg
     9781 ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat
     9841 agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat
     9901 agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggacagg
     9961 tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc
    10021 tttattccgc tcggcccagt cattcaagcg acgtttcgct tcgtcctgta gatgtttgcg
    10081 cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag
    10141 cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac
    10201 gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc
    10261 tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt
    10321 caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt tttcagcggc
    10381 cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc
    10441 ttccatacgt gtgtcggtca tctcaacatg cgcctgccct tgccagttgt ggcggaatgc
    10501 gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga
    10561 aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca gccgtggctg
    10621 gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctttcc
    10681 accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg
    10741 cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat
    10801 atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac
    10861 cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc
    10921 ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg
    10981 ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc
    11041 ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact gggactggaa
    11101 actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat
    11161 ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag
    11221 ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg
    11281 atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc
    11341 ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat caaacgacca
    11401 tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc
    11461 actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg
    11521 cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc
    11581 ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat
    11641 actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct
    11701 acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac
    11761 taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg
    11821 tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc
    11881 ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga
    11941 aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt
    12001 accggagttg tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc
    12061 ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac
    12121 cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg
    12181 aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc
    12241 ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa
    12301 ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg
    12361 cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca
    12421 cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg
    12481 cataccagct tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta
    12541 ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt
    12601 ccgcgtattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat
    12661 attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc
    12721 gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca
    12781 aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaacct
    12841 tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg
    12901 agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc gcgctgggat
    12961 ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc
    13021 agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa
    13081 gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc
    13141 actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg
    13201 ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat
    13261 agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt
    13321 gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac
    13381 gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga
    13441 aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca atcacagcat
    13501 gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt
    13561 atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg
    13621 ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga
    13681 attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc
    13741 tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg
    13801 ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg
    13861 gcattcggca gcgccattgt tccgcctctg ttggggggtg cccctattaa gttgtcaagc
    13921 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
    13981 gcagcgtcgt ctcgccaggg cgataagcac ctgattgtgt cgttttccct gactcatttt
    14041 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
    14101 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
    14161 gg
//
LOCUS       DADWTH010000100        13994 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.101, whole genome shotgun sequence.
ACCESSION   DADWTH010000100 DADWTH010000000
VERSION     DADWTH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.101"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..422
                     /locus_tag="JNY02_003177"
     CDS             <1..422
                     /locus_tag="JNY02_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434627.1"
                     /translation="DGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLK
                     GATAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAV
                     HIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
     gene            594..701
                     /locus_tag="JNY02_003178"
     CDS             594..701
                     /locus_tag="JNY02_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434628.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(754..1508)
                     /locus_tag="JNY02_003179"
                     /pseudo
     CDS             complement(754..1508)
                     /locus_tag="JNY02_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
     gene            1794..2723
                     /gene="pfkB"
                     /locus_tag="JNY02_003180"
     CDS             1794..2723
                     /gene="pfkB"
                     /locus_tag="JNY02_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBB9434629.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2824..3114
                     /gene="ghoS"
                     /locus_tag="JNY02_003181"
     CDS             2824..3114
                     /gene="ghoS"
                     /locus_tag="JNY02_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBB9434630.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3220..4080
                     /locus_tag="JNY02_003182"
     CDS             3220..4080
                     /locus_tag="JNY02_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBB9434631.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4121..4657)
                     /locus_tag="JNY02_003183"
     CDS             complement(4121..4657)
                     /locus_tag="JNY02_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBB9434632.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4804..5471
                     /gene="hxpB"
                     /locus_tag="JNY02_003184"
                     /pseudo
     CDS             4804..5471
                     /gene="hxpB"
                     /locus_tag="JNY02_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            5634..6224
                     /locus_tag="JNY02_003185"
     CDS             5634..6224
                     /locus_tag="JNY02_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBB9434633.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6357..7747
                     /gene="tcyP"
                     /locus_tag="JNY02_003186"
                     /pseudo
     CDS             6357..7747
                     /gene="tcyP"
                     /locus_tag="JNY02_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7751..8566)
                     /locus_tag="JNY02_003187"
     CDS             complement(7751..8566)
                     /locus_tag="JNY02_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434634.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINEDFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFA
                     AIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(8843..9106)
                     /gene="cedA"
                     /locus_tag="JNY02_003188"
     CDS             complement(8843..9106)
                     /gene="cedA"
                     /locus_tag="JNY02_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBB9434635.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9289..11550
                     /gene="katE"
                     /locus_tag="JNY02_003189"
     CDS             9289..11550
                     /gene="katE"
                     /locus_tag="JNY02_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBB9434636.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPRSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGHVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(11597..12355)
                     /gene="chbG"
                     /locus_tag="JNY02_003190"
     CDS             complement(11597..12355)
                     /gene="chbG"
                     /locus_tag="JNY02_003190"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBB9434637.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12368..13720)
                     /gene="celF"
                     /locus_tag="JNY02_003191"
                     /gene_synonym="chbF"
     CDS             complement(12368..13720)
                     /gene="celF"
                     /locus_tag="JNY02_003191"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB9434638.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RYTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(13825..>13994)
                     /locus_tag="JNY02_003192"
     CDS             complement(13825..>13994)
                     /locus_tag="JNY02_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9434639.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
BASE COUNT         3716 a         3452 c         3216 g         3610 t
ORIGIN      
        1 atgatggtga gccaggatta tacgccgcaa tggaaaataa tcaccctttg tgtgtcacac
       61 ggttcctctc taaaattaat ggcatcgcct ttaaatacaa gttgagcaaa gctaacatca
      121 tggatttatt aaaaggagct acagcacagg gaacccctgc attatacatc gccatgagca
      181 agggtaatga agacgtcgtg ttatcttata tatcgacgct gggtgctttt gcaaaaaaac
      241 attcttttag tcaacatcag ttatttacac tattggccgc taaaaatcat gacaacatgt
      301 cagctgttca tatagccatt catcataagc attataaaac tgtagaaaca tattatgctg
      361 ctataaatgt catcagccaa agcctgagtt ttagtgctga tgaaataaag acgtatttat
      421 aaccagctat ttacgatata tatcgataca taattttatt tttttcataa aacgcagaaa
      481 cagtgaccat gtgcacaacg ccacccgacg gctgtaataa caaaaaatcc cttgatgcct
      541 gtcccttttg ttacactccg ttatcacgca caagagatat gcaggacact ggtatgccga
      601 ctaaacgctt tgataaaaaa cactggaaga tggtggtggt gctactggca atctgtggcg
      661 ctatgttgtt gctacgttgg gcagcaatga tttggggctg agcagtgaca aacgaagaca
      721 gccagaccgc ataacacggt ccggcagata attttacatg ctgtagccca gcgataaggt
      781 tgtccgacgg tcggtatgtt ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt
      841 gtaagccacc ttcagtccaa aatgttcatt aatagcaaca tttaatgcgc tttcggagtt
      901 cagcgttgtg tcttccgcgc caaagaccga aacaccctgc gtaaatttag cgttgtcagt
      961 caactgccat gcataagccc cggaggcata gcccagcggc tgggtttcac tggcattgtc
     1021 ggtatatttg tcgtaacgca cacctggacc gaattcaaag cggaaactgt gtaccgggcc
     1081 attcaaaaac tgacgaccat aacccgcggt caacacatcg cgctctcgat agccgttata
     1141 acggtctgtc agccagcttg cctgaccaaa taaatagtca taatcagtta aattaaagcg
     1201 gctacgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1261 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1321 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1381 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1441 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     1501 agcttcattt atcaagagtc cgtacaacaa aaaagagacc atcacggtcc cggaaacttt
     1561 cttaaggatc aaagattagc gtccctggaa aggtaacgaa ttataaaaag gcgcgaataa
     1621 cttagcaatg tattcttatt tcattttttg aataagcatg tggcgaaaac agatttttat
     1681 ttatatatat ttatctgcaa aattttaaat aaagctctaa taaatcatat tgttaatttc
     1741 ttcactttcc gctgattcgg tgccagactg aaatcagcct ataggaggaa atgatggtac
     1801 gtatctatac gttgacactt gcgccctctc tcgatagcgc aacaattacc ccgcaaattt
     1861 atcccgaagg aaaactgcgc tgtaccgcac cggtgttcga acccgggggc ggcggcatca
     1921 acgtcgcccg cgccattgcc catcttggag gcagtgccac agcgatcttc ccggcgggtg
     1981 gcgcgaccgg cgaacacctg gtttcactgt tggcggatga aaatgtcccc gtcgctactg
     2041 tagaagccaa agactggacc cggcagaatt tacacgtaca tgtggaagca agcggtgaac
     2101 agtatcgttt tgttatgcca ggcgcggcat taaatgaaga tgagtttcgc cagcttgaag
     2161 agcaagttct ggaaattgaa tccggggcca tcctggtcat aagcggaagc ctgccgccag
     2221 gtgtgaagct ggaaaaatta acccaactga tttccgctgc gcaaaaccaa gggatccgct
     2281 gcatcatcga cagttctggc gaagcgttaa gtgcagcact ggcgattggt aacatcgagt
     2341 tggttaagcc taaccaaaaa gaactcagtg cgctggtgag tcgcgaactc acccagccag
     2401 acgatgtccg caaagccgcg caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg
     2461 tttcccttgg tccacaagga gcgctgggtg ttgatagtga aaactgcatt caggtggtgc
     2521 caccaccggt gaaaagccag agtaccgttg gcgctggtga cagcatggtc ggcgcgatga
     2581 cactgaaact ggcagaaaat gcctctcttg aagagatggt ccgttttggc gtagctgcgg
     2641 ggagtgcagc cacactcaat cagggaacac gtctgtgctc ccatgacgat acgcaaaaaa
     2701 tttacgctta cctttcccgc taacaaaaac attcccccag cattggggga atcaccacca
     2761 acctgtcggc aacgcgtttc tccgactatg ctcaaaagtc atgtgataac aaaggggtga
     2821 actatggcca gtggcgatct tgtccgttat gtcataaccg tcatgttgca tgaggataca
     2881 ttgactgaaa ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg
     2941 acggatgatg agggaaatat ccatgagctg gggactaaca cttttggact tatcagtacc
     3001 caaagtgaag aagaaattag agaactggtt tcggggctta cccaaagtgc aaccggcaaa
     3061 gatcctgaaa tcaccatcac gacctgggag gaatggaata gcaacagaaa ataaatggtt
     3121 tttgggcaat aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg
     3181 ctaaccttat gatctggcag acaacatggg agagacatca tgtggcaggc aatcagtcgt
     3241 cttttgagcg agcagttagg tgaaggcgaa atcgaactgc gtaatgaact gcctggcgga
     3301 gaagtccatg ccgcatggca tttgcgctat gcaggacatg actttttcgt caaatgtgat
     3361 gaaagggaac tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt
     3421 agtaaaaccg tcaccgtgcc taaggtttgg gcagtaggcg ctgaccgtga ctacagtttt
     3481 ctggtgatgg attatctccc acctcgtccg ctggatgcgc atagcgcatt tattcttggt
     3541 cagcaaattg cgcgtttaca tcaatggagt gaccaaccac aatttggcct cgattttgat
     3601 aactcgctct ccacaactcc acagcccaac acctggcaac gtcgctggtc aacgtttttt
     3661 gctgaacaac ggattggctg gcagttggaa ctggcagcag agaaagggat cgctttcggc
     3721 aatatcgacg ccatcgtcga gcatattcag cagcgtctgg cctcgcatca accgcagcct
     3781 tctctgttgc acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac
     3841 attttcgacc ctgcctgcta ctggggtgac cgagagtgcg acctggcgat gttaccgctg
     3901 catactgaac agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc
     3961 gatttccttg aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaagatta
     4021 tttggcggtc agcatttggt tattgctcag cagtcattgg atagattatt agcagcatga
     4081 tatgggttga ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag
     4141 aaaagaacac ataacctgca gcaatacaaa taaccggcag gatatatagt gagaaaaact
     4201 gcaggaaaat agtatggtgc ggaacaacaa tacgggactc aatttgttca cgcgttagcc
     4261 cctcttcccc tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc
     4321 gcgcctggcg actcatccgg gcaccggaat cctgcaacgc cagtccgaca aaaatcagga
     4381 tgaaaatcac ccagaacata acgttcacac caccattaaa attaggggtc ggggagttat
     4441 accagaacag attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagcaa
     4501 agtcgagcat gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca
     4561 gcaaagaaat caatgtagag atcaatgcag gtataaaaat cacccaaccc aaaatccttt
     4621 tcaaaacagc aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg
     4681 tcgtttcact tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga
     4741 taggcgctta atcataagca cggcttaata ccttacacat aatgctctaa aggagaggtt
     4801 gtaatgtcaa ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc
     4861 gactcagaac ctttatggga tcgagccgaa ctggatgtga tggcaagcct gggggtagat
     4921 atctcccgtc gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat
     4981 ctttgtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5041 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5101 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5161 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5221 cggttgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5281 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5341 cttctaaagc agcctgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5401 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     5461 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     5521 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     5581 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     5641 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     5701 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     5761 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     5821 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     5881 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     5941 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6001 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6061 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6121 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6181 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6241 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6301 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6361 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6421 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     6481 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     6541 tacagtggtt taacatcgtt ggtaacggca atgttcaact gctgcaaatg atcgttatgc
     6601 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     6661 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     6721 tcggtgtgct ggtcaccacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     6781 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag
     6841 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     6901 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     6961 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttaaccgcta tcgataccct
     7021 acaaagctga gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7081 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7141 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7201 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7261 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7321 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7381 tcagaacggc tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt
     7441 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     7501 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     7561 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     7621 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     7681 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     7741 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     7801 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     7861 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     7921 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     7981 caccacttcc cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg
     8041 atattatcga aggaaacttt aggagactct catttttttg aatcgcagca aaaatgctcc
     8101 cggcaggatg tatataattt ttttcatagt atatagtcct taaataatga ataaccaacc
     8161 tattttttat attgatcaaa ttcgtcattt tttcgggtcg gaattcacag gtaaaatcca
     8221 tcccaacaaa atcctcatta atatctctta tccgaaactc actgccataa agattattaa
     8281 ttatcactct atatttaaca ttgtcctgca gatacgtatc aaaactaagt gctggcgctc
     8341 ctagctttga ggcatcatag ttaatgttag acatataggc aatcatacga ttgaagttcg
     8401 actctgctat ttcatcgcag tcacattgac ccgttgatat aaagaaagtg agtgccgcca
     8461 tggtagactc aagcctttct ttaaaagagt cgaaagccag gcttttaata tttaaatcac
     8521 cataattact ctgtattaag tttgtagaaa acatctcccg cctcatattg ttaacaaaat
     8581 tattatctta tttaaatcta aaccatttac aatataagtt taagagcgac gccacaggat
     8641 gaactatcaa aaataactca tcatgattag caaaacttaa ccattttaaa ataaataaac
     8701 aattaaagaa aaaagatcac ttatttatag caatagctcg tcaaaggcag ctttttgtta
     8761 caggtggttt gaatgaatgt agcaacgaaa tgcagaattt caggtcctgt aactcccggc
     8821 aaaaccagga ggtatgtaat ccttactcag tcacttcccc ttcctggcgg atctgatttg
     8881 cccaacgttg ggcagattca ggcacagtaa acgccggtga gcgcagaaat gactctccca
     8941 tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct
     9001 ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt
     9061 tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta aaaattagaa
     9121 aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac
     9181 cgtttccaga atagtctccg aagcgggatc tggctggtgg tctatagtta gagagttttt
     9241 tgaccaaaaa agcggccctt ccagtaataa attaaggaga cgagttcaat gtcgcaacat
     9301 aacgaaaaga acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg
     9361 gggatggact cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg
     9421 ccaggtgcac aacctaccgc ccctaggagc ctgaaagccc ctgatacacg taacgaaaaa
     9481 cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag
     9541 ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg
     9601 ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa
     9661 cgtattgttc atgcacgcgg atcagcagct cacggttatt tccagccata taaaagctta
     9721 agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt
     9781 aggttctcta ccgttcaggg tggcgctggc tctgccgata ccgtgcgtga tatccgtggt
     9841 tttgccacca agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca
     9901 atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa
     9961 ccgcactggg caattccaca agggcaaagt gcccacgata ctttctggga ttatgtttct
    10021 ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc
    10081 agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg
    10141 aaggcaacgt ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg
    10201 gatgaagcac aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa
    10261 gccattgaag caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa
    10321 gatgaattca agttcgactt cgatcttctc gatccaacca aactgatccc ggaagaactg
    10381 gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc
    10441 gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac
    10501 gatcccctgt tgcagggacg tttgttctcc tataccgata cacaaatcag tcgtcttggt
    10561 gggccgaatt tccatgagat tccgattaac cgcccgacct gcccttacca taatttccag
    10621 cgtgacggca tgcatcgtat ggggatcgac actaacccgg cgaattatga accgaactcg
    10681 atcaacgata actggccgcg cgaaacacca ccggggccga aacgcggcgg ttttgaatca
    10741 taccaggagc gcgtggaagg caataaagtt cgcgagcgca gcccatcgtt tggcgaatat
    10801 tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc
    10861 gatggtttca gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt
    10921 gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc
    10981 gaactgactg acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag
    11041 gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtca cgtggtagcg
    11101 attttactta atgatgaagt gagatcggca gaccttcttg ccattctcaa ggccctgaag
    11161 gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac
    11221 ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg
    11281 gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactat
    11341 ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt
    11401 aaagcaacaa tcaaggtagc tgaccagggt gaagaaggga ttgtggaagc tgacagcacc
    11461 gacggtagtt ttatggatga actgttaacg ctgatggcag aacaccgcgt gtggtcacgc
    11521 attcctaaga ttgacaaaat tccggcgtaa atatggataa ggtcacgtcc cctggggagt
    11581 gaccttacca ccttacttac acatcaagat aactacccaa ccgataacca cgctcagcaa
    11641 ttgcatattt caacgacgct gatgtcagca cttccagctc cgttaaccgt gggaaacagt
    11701 aagcactctg acggatagta ttatcgataa acgccggatg acacatcacc tccagcgaag
    11761 gatcaccccg atggcttgcg tcatccagca cttgcaggaa cagtgcctcg ctaatctctt
    11821 caccataaaa cgcgctactg aatccctgcg aactgcgcag attagccggt aaatcaccag
    11881 cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg
    11941 caacaatcgg gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag
    12001 gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt
    12061 cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat
    12121 cgcgggttaa ccccggcata gctgtcagtg gcttgcccat agtaaggaca aagtgcatcc
    12181 ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat
    12241 tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt
    12301 tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca
    12361 gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt
    12421 tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca
    12481 acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc
    12541 tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat
    12601 gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag cttacaggtc atttctaccg
    12661 cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttcag
    12721 cttgcttgtc gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc
    12781 caccgcgctg ttccagttct ttcggcttaa ctttcaactc cgggttttta tacagctcaa
    12841 aaagttgttt ctcgactttc tgtactacct gcgctcgtgc gccgcctttg tagtattcgc
    12901 ccatttcaat agccagcatc tctttttgct tgaagtaata aagcaaataa gaacacggca
    12961 acagattcag agaacgaatt aagccttcgc taaatggcag atcgaaaata tttttaacgc
    13021 cagatgcttt taactgcccg gatgccacac catcaagcaa ttcggcaaag cgcgacttgc
    13081 catttaccag cacattctta atgaacacca tatggttgag gccaaacaaa tcgatagata
    13141 aatcatcgct gtctttcagc atcagaacat cgtgaataaa catcttcatg ccgatcggaa
    13201 tattacacac gccgataaag cgtttaaatc cggtataacg ataaacggct tcagtaacca
    13261 ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt
    13321 ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacaaaccg cccgcgccgt
    13381 tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt tcacgcgccg
    13441 gtaattggcc aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc
    13501 aatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga
    13561 gatcgaaaat aatatccagt ttagctttgc caccctcgac atccaccagc cataattcgc
    13621 tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc
    13681 tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag
    13741 aatactgata tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat
    13801 atgtgaattg tcaggtataa cgacttactg attaaattcc gtcaatttct tacgatatct
    13861 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata
    13921 accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt gttttttggc
    13981 aaaattaata cgga
//
LOCUS       DADWTH010000101        13988 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.102, whole genome shotgun sequence.
ACCESSION   DADWTH010000101 DADWTH010000000
VERSION     DADWTH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.102"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..454
                     /locus_tag="JNY02_003193"
                     /pseudo
     CDS             1..454
                     /locus_tag="JNY02_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            514..621
                     /locus_tag="JNY02_003194"
     CDS             514..621
                     /locus_tag="JNY02_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBB9434640.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNY02_003195"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNY02_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBB9434641.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNY02_003196"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNY02_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNY02_003197"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNY02_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNY02_003198"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNY02_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBB9434642.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNY02_003199"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNY02_003199"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBB9434643.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="JNY02_003200"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="JNY02_003200"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBB9434644.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNY02_003201"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNY02_003201"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBB9434645.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="JNY02_003202"
     CDS             complement(9943..10530)
                     /locus_tag="JNY02_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBB9434646.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="JNY02_003203"
     CDS             10700..11278
                     /locus_tag="JNY02_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBB9434647.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="JNY02_003204"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="JNY02_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="JNY02_003205"
     CDS             complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="JNY02_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBB9434648.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            13869..13952
                     /gene="yohP"
                     /locus_tag="JNY02_003206"
     CDS             13869..13952
                     /gene="yohP"
                     /locus_tag="JNY02_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBB9434649.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3333 a         3905 c         3527 g         3223 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgcc cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attcgccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccaaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    13921 tggtgactgg cgtatttaag atgatatttt aaaattaatt aatgtcatca gatccgaaaa
    13981 taacgaga
//
LOCUS       DADWTH010000102        13826 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.103, whole genome shotgun sequence.
ACCESSION   DADWTH010000102 DADWTH010000000
VERSION     DADWTH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.103"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            166..1419
                     /gene="kch"
                     /locus_tag="JNY02_003207"
     CDS             166..1419
                     /gene="kch"
                     /locus_tag="JNY02_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBB9434650.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1474..1647)
                     /locus_tag="JNY02_003208"
     CDS             complement(1474..1647)
                     /locus_tag="JNY02_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBB9434651.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1790..3250
                     /gene="cls"
                     /locus_tag="JNY02_003209"
     CDS             1790..3250
                     /gene="cls"
                     /locus_tag="JNY02_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBB9434652.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3285..3614
                     /locus_tag="JNY02_003210"
     CDS             3285..3614
                     /locus_tag="JNY02_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBB9434653.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="JNY02_003211"
     CDS             complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="JNY02_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBB9434654.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="JNY02_003212"
     CDS             complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="JNY02_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBB9434655.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLRACFKPVE
                     ELL"
     gene            complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="JNY02_003213"
     CDS             complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="JNY02_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBB9434656.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="JNY02_003214"
     CDS             complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="JNY02_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBB9434657.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="JNY02_003215"
     CDS             complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="JNY02_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBB9434658.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="JNY02_003216"
     CDS             complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="JNY02_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBB9434659.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11115..13790
                     /gene="adhE"
                     /locus_tag="JNY02_003217"
                     /gene_synonym="adhC"
     CDS             11115..13790
                     /gene="adhE"
                     /locus_tag="JNY02_003217"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBB9434660.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3627 a         3426 c         3221 g         3552 t
ORIGIN      
        1 gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt tgattactgc
       61 ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt tcagaaaatg
      121 tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag tcactgggct
      181 acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat tctcgcgctg
      241 gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc ggtcaatttg
      301 cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct ggcaaacgcc
      361 ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact gttccgggca
      421 aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta caccctgcat
      481 ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt gtttttgctg
      541 atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt tgcatttatt
      601 agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag cgaaggtttt
      661 aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac catgtcaacc
      721 gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac tatttcggtc
      781 attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg tccgcttatc
      841 cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg taaagatcat
      901 tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact gaatcaacgc
      961 ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca acttgagcaa
     1021 cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc agtattaaag
     1081 aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga tgcagataac
     1141 gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc
     1201 agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat tctctcaccg
     1261 caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat taataatgat
     1321 atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa cgatgaacaa
     1381 gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac ggaagagtaa
     1441 acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg caaacaataa
     1501 cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg
     1561 cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg
     1621 ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat
     1681 tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat
     1741 ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt
     1801 ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac
     1861 tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat
     1921 ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg
     1981 caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga
     2041 ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt
     2101 caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat
     2161 gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa
     2221 tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc
     2281 attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact ccgccgggag
     2341 tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt
     2401 cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca
     2461 tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga
     2521 tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat
     2581 ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac
     2641 cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg aacaggccag
     2701 tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca
     2761 ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca cgccctactt
     2821 tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt
     2881 cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt
     2941 ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca
     3001 taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta accttgatat
     3061 gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag gttttggtgc
     3121 agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt
     3181 atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagccc
     3241 gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa
     3301 acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt ctcgaactgg
     3361 ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg
     3421 gcggcgcgga gttattcgat ccggtggaag actggcagga acatgttgat ttcgatttga
     3481 acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat ggtgaaatca
     3541 acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca
     3601 tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg tcagccctta
     3661 ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccctccag
     3721 aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca
     3781 accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt
     3841 cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg
     3901 tagtggattg tggtagacct catcataggt ccccagttct actgcatggc cgagatacat
     3961 caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa taaaaattaa
     4021 tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg cctgaattga
     4081 cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa gaataagagc
     4141 acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat
     4201 caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg
     4261 gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat
     4321 acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac tgcgaacggc
     4381 acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc
     4441 ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc atcccgattc
     4501 ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt
     4561 gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt
     4621 aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc
     4681 tccaccggtt taaagcaagc acgcagacgg ccaagcgtaa actcttccag cggcggtgcg
     4741 ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat
     4801 cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga
     4861 cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg
     4921 cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc
     4981 cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa ttcccgcttc
     5041 agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc tgtagtgggt
     5101 tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat gactcgctga
     5161 cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc ttccggcatt
     5221 tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta
     5281 tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt caacgaagtc
     5341 attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcacgctct
     5401 ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg tccgttggca
     5461 gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca
     5521 atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg
     5581 ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt
     5641 tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact ccttaacgat
     5701 ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagtgtca
     5761 ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc gagttcgcgc
     5821 catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac cccaggaagc
     5881 taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag accaccacca
     5941 caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat acaccgccaa
     6001 cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac
     6061 gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg atgttttggc
     6121 cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca
     6181 gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa
     6241 gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc
     6301 cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac
     6361 cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact
     6421 gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg
     6481 caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt
     6541 cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca
     6601 tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac
     6661 aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta acactaagga
     6721 atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat
     6781 ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca tataggagag
     6841 cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc
     6901 tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag agccacgggt
     6961 aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa
     7021 tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat
     7081 catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc
     7141 catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac
     7201 acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa cgggaaaact
     7261 ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcgcc
     7321 atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata
     7381 tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg taaaagggct
     7441 tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat
     7501 cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct
     7561 tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc
     7621 attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg
     7681 tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa
     7741 tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct
     7801 cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg
     7861 cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt
     7921 ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg tcgaggaacg
     7981 ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag
     8041 aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg ttgatggtca
     8101 acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt
     8161 tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca gtatacggtg
     8221 gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc
     8281 ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt
     8341 tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct
     8401 cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg tcgatttcac
     8461 cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt
     8521 taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt
     8581 ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc
     8641 atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa
     8701 gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa
     8761 tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg
     8821 cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac
     8881 gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat
     8941 ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc
     9001 caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga
     9061 tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat
     9121 tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca
     9181 gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg
     9241 tgatgttggt cattgttttt tggactccct cattataatt actggtatta accagcatgt
     9301 gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat
     9361 cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat caccttatgt
     9421 cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg
     9481 ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc
     9541 ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt attgaccgta
     9601 tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag
     9661 attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg cacaataatt
     9721 taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt actaatatca
     9781 tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag
     9841 gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta
     9901 ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga
     9961 gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt
    10021 aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt
    10081 aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa
    10141 tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat
    10201 gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa
    10261 cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg atttttcttg
    10321 cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac
    10381 caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt gtagaatcgt
    10441 gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt
    10501 agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat
    10561 ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc
    10621 aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat
    10681 aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac
    10741 ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac
    10801 caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat gctgaaaggt
    10861 gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt gagtaatctt
    10921 gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta aactctttta
    10981 gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcattggtt aagcaaatca
    11041 tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa gtttaacatt
    11101 atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc gtagagcgtg
    11161 taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac aaaatcttcc
    11221 gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg gccgttgccg
    11281 aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct tctgaatata
    11341 tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac gacacttttg
    11401 gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg accactaacc
    11461 cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac gccattatct
    11521 tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc gttctgcagg
    11581 ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg
    11641 aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg actggtggtc
    11701 cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta ggcgcgggta
    11761 acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca tctgtactga
    11821 tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt gttgttgttg
    11881 actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat ctgttgcagg
    11941 gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg aacgcggcta
    12001 tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta ccagaaaaca
    12061 ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa
    12121 aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag
    12181 cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac actgaccagg
    12241 ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct cgtatcctga
    12301 ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt
    12361 ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac gttggtccga
    12421 aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg tggcacaaac
    12481 ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat gaagtgatta
    12541 ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac aatggttatg
    12601 ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc ttcttcgaag
    12661 tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca aactccttca
    12721 aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg aagatcatgt
    12781 gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc tttatggata
    12841 tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg atcgctgtca
    12901 ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact gacgacgcta
    12961 ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg attgtcgacg
    13021 ccaacctggt tatggacatg ccgaagtccc tttgtgcttt cggtggtctg gacgcagtaa
    13081 ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat ggtcaggctc
    13141 tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa
    13201 atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc gcatttgcga
    13261 acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag ttccatattc
    13321 cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat gcgaacgaca
    13381 acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc cgtcgttatg
    13441 ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct gctaagatcg
    13501 agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg aaatctatcc
    13561 gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg tctgaagatg
    13621 cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac
    13681 agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa
    13741 aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa tcagtagcgc
    13801 tgtctggcaa tataaacggc cccttc
//
LOCUS       DADWTH010000103        13703 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.104, whole genome shotgun sequence.
ACCESSION   DADWTH010000103 DADWTH010000000
VERSION     DADWTH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.104"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(511..1602)
                     /gene="ychF"
                     /locus_tag="JNY02_003218"
     CDS             complement(511..1602)
                     /gene="ychF"
                     /locus_tag="JNY02_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBB9434661.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1719..2303)
                     /gene="pth"
                     /locus_tag="JNY02_003219"
     CDS             complement(1719..2303)
                     /gene="pth"
                     /locus_tag="JNY02_003219"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBB9434662.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2581..2859
                     /gene="ychH"
                     /locus_tag="JNY02_003220"
     CDS             2581..2859
                     /gene="ychH"
                     /locus_tag="JNY02_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBB9434663.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="JNY02_003221"
     CDS             complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="JNY02_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBB9434664.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4718..5665)
                     /gene="prs"
                     /locus_tag="JNY02_003222"
     CDS             complement(4718..5665)
                     /gene="prs"
                     /locus_tag="JNY02_003222"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBB9434665.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="JNY02_003223"
     CDS             complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="JNY02_003223"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBB9434666.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="JNY02_003224"
     CDS             complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="JNY02_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBB9434667.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7504..8760
                     /gene="hemA"
                     /locus_tag="JNY02_003225"
     CDS             7504..8760
                     /gene="hemA"
                     /locus_tag="JNY02_003225"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBB9434668.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8802..9884
                     /gene="prfA"
                     /locus_tag="JNY02_003226"
     CDS             8802..9884
                     /gene="prfA"
                     /locus_tag="JNY02_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBB9434669.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9884..10717
                     /gene="prmC"
                     /locus_tag="JNY02_003227"
     CDS             9884..10717
                     /gene="prmC"
                     /locus_tag="JNY02_003227"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBB9434670.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10714..11106
                     /locus_tag="JNY02_003228"
     CDS             10714..11106
                     /locus_tag="JNY02_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBB9434671.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11110..11919
                     /locus_tag="JNY02_003229"
     CDS             11110..11919
                     /locus_tag="JNY02_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBB9434672.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEEPISEMIRAQINNIAHKHIVLH"
     gene            11955..12809
                     /gene="kdsA"
                     /locus_tag="JNY02_003230"
     CDS             11955..12809
                     /gene="kdsA"
                     /locus_tag="JNY02_003230"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBB9434673.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12958..13065)
                     /locus_tag="JNY02_003231"
     CDS             complement(12958..13065)
                     /locus_tag="JNY02_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBB9434674.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13494..13601)
                     /locus_tag="JNY02_003232"
     CDS             complement(13494..13601)
                     /locus_tag="JNY02_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBB9434675.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3403 a         3461 c         3527 g         3312 t
ORIGIN      
        1 attttcgctt gatgtatctg cataacaaaa acaatatgcg ccatttttgc attactaata
       61 agaaaaagca aaaaacatcg atattcattt ttggtcaatg gattttctgg cctatcgtta
      121 ttattgtgct actggttatc attttcatct tgccaggatc gcacgtaatc acgttattcg
      181 gggactttat ctgcgggata ttacccttca ggtaagtcct tttctcgcca gcatattgga
      241 gcgagatcaa tattgatcag ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg
      301 ttatatcact gctaatgaga tatcagtcat ctgaacactt atgtctttaa ttattctcgt
      361 ggttcactgt aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg
      421 tgaaatgaaa aatccacgca attgcgtgga ttttatatac ttttgcgctc ttcatgagat
      481 ttagcgaaac ctcatgagac aataaattaa ttagacgttg aaaaggaagt tcatcacatc
      541 gccatctttc acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc
      601 ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc
      661 tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat
      721 ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt
      781 ataaccggca cggatcacac ggttcaggcc cggttcttcc agcccaagct cctgcataaa
      841 ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg
      901 aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt
      961 ttcaaaaccg tcttcgttga cgttggctat gtacattgtt ggtttcagcg tcaggaagct
     1021 caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc
     1081 gttttccagc tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc
     1141 gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc
     1201 cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac
     1261 gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg
     1321 gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc
     1381 ggcgatatcg acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc
     1441 cagttgatcc aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca
     1501 gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt
     1561 cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat
     1621 atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat
     1681 tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc
     1741 aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct
     1801 tcgtcgatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa
     1861 ccgacaactt tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta
     1921 gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg
     1981 cccaacttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt
     2041 tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc
     2101 ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagggtgac tcgcgaagta
     2161 taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca
     2221 acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg
     2281 ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta
     2341 gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata
     2401 attgcctgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt
     2461 tgaaataagg gttgtaattg tgattacgcc cgcacataac ccacagggtg ttgtctatac
     2521 tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat
     2581 atgaaacgca aaaacgcttc gatactcggt aacgtgctca tggggttggg tctggtggta
     2641 atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag
     2701 tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt
     2761 gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg tcactatgac
     2821 aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac
     2881 cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg
     2941 attcgggaag aacgcgaggc gtcccgggat cggttgaatg cctgcgcgag ccatagttcg
     3001 cagtggctgg aattccacgt tgcacacgcg cagttcacat ccttcgggca gacgcttcac
     3061 aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaacggcat cccacttcag
     3121 aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaataagc cttcagcagc
     3181 agcaaaaaac agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac
     3241 taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac
     3301 gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcagtgaca tacacagcag
     3361 catgacgatg atgtcatctt tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc
     3421 actcatgttc cacgccacca tcaacagcag ggctgccatg gcggaaagcg gcagccagga
     3481 gagcagtggt gccagtacca gcagggcgag aataaccaga atagagtgga tcaccgccga
     3541 gataggggaa gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc
     3601 ggtaatacca ccaaagaatg gagcgatgat attccccagc ccctgaccga ccagttcact
     3661 attcgccttg tgcttagtcc cggtcatacc atccagtacc acagcgcaga gcagagattc
     3721 gatcgccccg agcattgcca ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca
     3781 ggttagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg gcagcagttg
     3841 cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt
     3901 agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc
     3961 cggaaggtga ccgggtaaac gaatgcccag acgcggccag aaaaccagaa tgccaagcgt
     4021 cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc
     4081 gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat
     4141 ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg
     4201 aatatactca atcagacgac caaagcgtgc cagccccatc agaatcaaaa agattcccga
     4261 caacaaggtc gcaaccagca gtcctgccag tccaaactgt tgcgaaacgg gatagagaat
     4321 taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gacccacccg ttagagcaat
     4381 gacaatcccc gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat
     4441 agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc
     4501 acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa
     4561 aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca
     4621 tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa
     4681 aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga
     4741 tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag
     4801 tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga
     4861 cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag
     4921 tcgcgtacgc aaatacacgt ttagcaccac gttctttcag cgcttcagca gctttacaca
     4981 gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac
     5041 cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca
     5101 tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg
     5161 gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc
     5221 taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat
     5281 ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag
     5341 tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga
     5401 taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt
     5461 ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc
     5521 gtacattttc gttaatttgt acgctgactt cgccgtcgct aaagcgacct acagcggcgt
     5581 cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagtaccggg gtggcgttac
     5641 cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa
     5701 gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc
     5761 gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa
     5821 gcatggctct gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca ttgagccatt
     5881 ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac
     5941 aggcccctgt cccagtcagg cgcgacgggg cgtattttaa cagccaggaa agcaccgcat
     6001 caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta
     6061 gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca
     6121 ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca
     6181 ccggcgttag tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag
     6241 gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt
     6301 gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac
     6361 cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac
     6421 gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt
     6481 catgttccac gccttcaacg ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa
     6541 tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat
     6601 ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg
     6661 tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt
     6721 gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac
     6781 cttccagttt ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag
     6841 tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg
     6901 aattggcatt ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg
     6961 acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct
     7021 gcccaatggg ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa
     7081 gcgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg
     7141 attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc
     7201 aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag
     7261 gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca
     7321 gttataaccc ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat
     7381 tgccagaatc taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc
     7441 ttacgcatcc tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca
     7501 gacatgaccc ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa
     7561 cgtgtatcgt tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg
     7621 atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt
     7681 gaagagcagg ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt
     7741 aatgaagaag atctgcgtaa aaacctctac tggcatcagg ataacgacgc ggttagccat
     7801 ttaatgcgtg ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt
     7861 caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag cgaactggaa
     7921 cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac agatatcggt
     7981 gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg
     8041 ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt
     8101 catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc
     8161 caaattctgg cagatgaagt tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt
     8221 ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa
     8281 ggcatggtgg agcgcgcatt aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt
     8341 gccgttccgc gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagt
     8401 gtggacgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt
     8461 gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa
     8521 agcgccagcg aaaccattcg cgagtatcgc agccaggcag agcacgttcg cgatgagtta
     8581 accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat
     8641 ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc
     8701 gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag
     8761 cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg
     8821 ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc
     8881 aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg
     8941 atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga
     9001 tgatgctcga cgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag
     9061 aaaaaagcga gcaactggaa cagcaattac aggttctatt actaccaaaa gatcctgatg
     9121 acgaacgtaa cgccttcctc gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt
     9181 ttgcaggcga tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag
     9241 aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta
     9301 gcggtgatgg tgtgtatggt cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg
     9361 ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc
     9421 cagaactgcc tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt
     9481 tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta
     9541 ctcacttgcc gaccgggatt gttgttgaat gtcaggacga acgttcacaa cataaaaaca
     9601 aagcgaaggc gctttcagtg ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc
     9661 aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta
     9721 accgtactta caatttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct
     9781 accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg
     9841 aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg
     9901 gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat
     9961 cctgctggag catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca
    10021 gctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga
    10081 acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttctcc
    10141 agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt
    10201 gcctgaacaa ccttgccgta ttctcgatct cgggacgggt accggggcga ttgcgcttgc
    10261 gctggctagc gagcgcccgg actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt
    10321 ctccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag
    10381 cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta
    10441 tattgacgag caggacccac atcttcaaca aggcgatgtc cgctttgagc cgctcactgc
    10501 gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa
    10561 cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt
    10621 gcgacaagca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga
    10681 taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt
    10741 gttcatctta ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt
    10801 tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat
    10861 acggtgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttcc
    10921 gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt
    10981 tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg
    11041 ctggcgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg
    11101 gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc
    11161 atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa
    11221 ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag
    11281 cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc
    11341 gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt
    11401 gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg
    11461 ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg
    11521 ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag
    11581 ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt
    11641 gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg
    11701 gagctggcgt tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa
    11761 attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat
    11821 ttaagttatt tcgttgaaca gtgtccggaa gagccgatca gcgaaatgat ccgtgcgcaa
    11881 ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag
    11941 attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg
    12001 acctgccgtt cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc
    12061 gcatttgcga gcactacgta actgtgaccc agaaactggg tatcccttac gtgttcaaag
    12121 cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag
    12181 aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg
    12241 ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg
    12301 cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta
    12361 acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca
    12421 aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca
    12481 acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg
    12541 tgattttcga cgtgacccac gcactgcaat gccgcgatcc gtttggcgca gcttccggtg
    12601 gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc
    12661 tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc
    12721 cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag
    12781 gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg
    12841 ttctgcgtta agactttttt catgggtgcc agatacaaaa aaggccgcag gctgttaccc
    12901 ctgcggccgg tttcgggcgc atattgccat cacggcagcc tgacgcccgt tttcacctta
    12961 cttccggtta cgccaccagc cgacaatcgc tgcggtaata attcccgcca ggatcggtgc
    13021 tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc gtcatatagc cgccttgttg
    13081 taatgacaac attttgcggc tattcttgaa ttgttctggt tcaagattag cccccgttct
    13141 gttgtcaggt taaacctctc aacgtgcggg ggttttctct ttccagcaac caatgccacc
    13201 agggataaag cccccgcaac attgcgcctc accggataac gccggcttga tgtggatact
    13261 acgtctcaat tcatcttcac ttcatccctg aaatgtttgc aatgaagagt gcattccggt
    13321 ttttcaacag ctgttacagt cgtttcatga gtgctctgga tgatgcttcc agctcgggtt
    13381 gccaatattt acttgtggaa gagataaaga caaaaatggc cgcaggcatg ttacccatgc
    13441 ggccggtttc gggcgcatat tgccatcacg gcagcctgac gcccgttttc accttacttc
    13501 cggttacgcc accagccgac aatcgctgcg gtaataatcc ccgccaggat cggtgctgcc
    13561 aggtcgtgcc agaaaatcat ggcaaacttc gcgagcgtca tatagccgcc ttgttgtaat
    13621 gacaacattt tgcggctatt cttgaattgt tgatggttca agattagccc ccgttctgtt
    13681 gtcaggtttt acctctcaac gtg
//
LOCUS       DADWTH010000104        13577 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.105, whole genome shotgun sequence.
ACCESSION   DADWTH010000104 DADWTH010000000
VERSION     DADWTH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.105"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            112..693
                     /locus_tag="JNY02_003233"
                     /pseudo
     CDS             112..693
                     /locus_tag="JNY02_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            711..1349
                     /locus_tag="JNY02_003234"
                     /pseudo
     CDS             711..1349
                     /locus_tag="JNY02_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1640..2368
                     /gene="mpaA"
                     /locus_tag="JNY02_003235"
     CDS             1640..2368
                     /gene="mpaA"
                     /locus_tag="JNY02_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBB9434676.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2343..3302)
                     /gene="ycjG"
                     /locus_tag="JNY02_003236"
     CDS             complement(2343..3302)
                     /gene="ycjG"
                     /locus_tag="JNY02_003236"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBB9434677.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3421..3927
                     /gene="tpx"
                     /locus_tag="JNY02_003237"
     CDS             3421..3927
                     /gene="tpx"
                     /locus_tag="JNY02_003237"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBB9434678.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3971..5512)
                     /gene="tyrR"
                     /locus_tag="JNY02_003238"
     CDS             complement(3971..5512)
                     /gene="tyrR"
                     /locus_tag="JNY02_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBB9434679.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5660..6721)
                     /locus_tag="JNY02_003239"
     CDS             complement(5660..6721)
                     /locus_tag="JNY02_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBB9434680.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6718..8115)
                     /locus_tag="JNY02_003240"
     CDS             complement(6718..8115)
                     /locus_tag="JNY02_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBB9434681.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8270..9268
                     /locus_tag="JNY02_003241"
     CDS             8270..9268
                     /locus_tag="JNY02_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB9434682.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9379..10284)
                     /gene="ompG"
                     /locus_tag="JNY02_003242"
     CDS             complement(9379..10284)
                     /gene="ompG"
                     /locus_tag="JNY02_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBB9434683.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10329..11411)
                     /locus_tag="JNY02_003243"
     CDS             complement(10329..11411)
                     /locus_tag="JNY02_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB9434684.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(11425..12084)
                     /gene="pgmB"
                     /locus_tag="JNY02_003244"
     CDS             complement(11425..12084)
                     /gene="pgmB"
                     /locus_tag="JNY02_003244"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBB9434685.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(12081..>13577)
                     /gene="ycjT"
                     /locus_tag="JNY02_003245"
     CDS             complement(12081..>13577)
                     /gene="ycjT"
                     /locus_tag="JNY02_003245"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBB9434686.1"
                     /translation="MCAQQSYDQHLAASTENWRQWWQKRRITVNGGDAHDQQALDYAL
                     YHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRW
                     HNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLV
                     ADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYT
                     EHVNNNAFTSYMVYYNVQQALSIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQ
                     DDSFMTKPAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSSA
                     SCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDD
                     GIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDA
                     QRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE"
BASE COUNT         3364 a         3681 c         3246 g         3286 t
ORIGIN      
        1 ccccaccaga ccctgtgaca tcaccccccg gatcatggac gactgggggc agtaatcatg
       61 ccgggtctgg ctggctaaca ccccattata aggggtacga ataaagtaac gggtggctat
      121 gccattaatg ccgcgctgac agaaaaacga attaaagctg tagtgagtat tacgggtgtt
      181 aatatcgggc gtctttttcg ggaaggtttc agcaattatg atccgattgg cgtattgaat
      241 gcaatggcgt cacaacgaac aaaagaggct cgtggtggag aattacagat caacgaatta
      301 cttcccgcca gcctggatgc agcaaaagcc catggactga ccgaacgcga tgtttatgaa
      361 gcaactgact actataaaac accacgcggg caacaaccgg gaggtgcggc aaaaatgttg
      421 ttctcacatg cacagaaaac gctggcctgg gatgccttcg catttactga agtgttactg
      481 acccagccgg tgatggtagt agttggtgaa aaagtcggtg ccttcggtgc ttatcgtgat
      541 ggactggaag tctatggccg ggcgatggtt tcgcaggatc gtcagttagt ttcgttaccc
      601 gatttctcac attatgaact atatgacaaa ccggaagcag ttcaggaggc gctggcaaaa
      661 gttattcctt ttttcaacac ccatctggga taagtttcat gagaaatatt atgaaaaatg
      721 tactgattct tggtgctggc ggtcagatag cccgccatgt gattaatcaa ctggcagata
      781 aacaaactat taaacaaact ttatttgccc gtcagccagc aaaaatacac aaaccctatc
      841 caactaatag ccagattatt atgggggatg tactgaatca tgcagcactg aagcaggcca
      901 tgcagggaca ggatattgtg tatgccaacc ttacggggga agatttagat attcaggcaa
      961 attcagtgat cgctgcgatg aaagcctgtg atgttaaacg tctgatcttt gttctttccc
     1021 tcggtattta tgatgaagtt ccaggaaaat ttgtcgaatg gaataatgca gttattggtg
     1081 aacccctaaa accattccgc agggctgcgg atgcaattga ggcttctgga ctggaatata
     1141 caattcttcg ccctgcctag ctgactgatg aagatattat tgattatgag cttacctctc
     1201 gcaatgaacc attcaaaggt acgatcgtat cacgtaagag tgtcgcagca ttaattacag
     1261 atatcatcga taaacctgag aaacacattg gtgagaatat aggcatcaat caacccggga
     1321 ctgatggcga taagccattc ttcatgtaaa tcaattatat agataccatc gtactgtact
     1381 atgtgcagta cgatttatta aacttattaa ggtgaactga tcaatcagat gcgattaaca
     1441 tgtgtagaac cagtaagaat aaaatacgcc atgtatataa cattacgaca attctaatat
     1501 ctgcacatca atgaaaatgc aaacaacttc cggcaaaaaa gtgatacaag atcgatgaca
     1561 tagcgttgaa gaaaaggatt tgataatccg caaaatttgc acaacactgc ctgtaatact
     1621 tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg gcgcatttcc
     1681 gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct ggtttcctgc
     1741 ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg atgaaaactc
     1801 ttcggtcgtc acgctctcct gtgctctgcg gactttgaca ccttctttgc gccgccatca
     1861 tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca atgctaatgg
     1921 tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa cggtttatcg
     1981 ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca aacctggctc
     2041 cgaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg cctgggtggt
     2101 ctcattccac gatccactgg cttgtattga agatcccaga cacagcgaat taggcgaatg
     2161 gctggcccag tcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc
     2221 tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt ttccgcccat
     2281 ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca
     2341 tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc taccgccagc
     2401 caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa agcggcgcta
     2461 atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg tgcacgcgct
     2521 tcagtcgcca gcgcttccgt cagaccaccg gttttatcga gcttaatgtt aaccatctca
     2581 tagcgccctt tcagcgcctt cagattgcta cgagtatgac aactttcatc agcacaaatc
     2641 ggcaacggat gaataaaatt ctccagcgcc gcatcgtcct gcgcaggaag cggttgttca
     2701 agcatcgcaa cgcctaaatc cgccaatagc tggcaacgcg ccgccaaccc ttctgcacgc
     2761 caggattcat ttgcatcaac gatcagcgtg gcatcgggca cagcggtgcg aattgccacc
     2821 atccgctcac tgataagatg gttatccagc ttcactttca gtaatttcgc gcctgcctgc
     2881 cagagtgttg atgcactatt ggccatctga tcaggcgtac cgatgacaac cgtctgtgca
     2941 gtgataactg tctcgggaag cgttatgccg atcaaatcag ccagcgattg ctgctgtttt
     3001 cgcgccgcga gatcccacaa tgcacaatcc agcgcattac gtgctgcgcc ggcagggaga
     3061 attttttgca actcctcccg tgtcagccct ttctctaatt gcggcacgac gctcataatt
     3121 tgcgccatta ccgaggcatc actttctcca taacgcggat acggcgtgca ttcgccggtg
     3181 cctttaatac cctcttcttc cagttcaacc accaccacgc gcgcttcact gcggcttccc
     3241 cgggcaatca caaacggggt atgtaagggc caggcttcct caaatacctt aacgattctc
     3301 atctttactc cttgttgtcg cggtggtctg cggattaagc gcaaatagcg tttgctgtgt
     3361 tattgacagt tagcataaac taggtgtgac gttaactata tgtaaacagg aaagataatc
     3421 atgtcacaaa ccgttcattt ccagggcaac ccggttacag tcgccaattc catcccgcag
     3481 gcgggtagca aagcgcagac ttttactctc gtggcaaaag atctgtctga cgtcaccctc
     3541 ggtcagtttg cgggtaaacg caaagtgctg aacattttcc cgagtattga taccggtgtt
     3601 tgcgccgcat cagtacgtaa gtttaaccaa ctggcaactg agatcgacaa caccgttgtg
     3661 ctgtgtatct ctgccgatct gccgttcgcc cagtctcgtt tctgcggcgc agaaggtctg
     3721 aacaacgtta tcaccctctc cactttccgt aacgctgaat ttctgcaagc ctacggtgtg
     3781 gcaattgctg atggcctact gaaaggtctg gcagcgcgtg ccgttgtggt tattgacgaa
     3841 aatgacaatg tgattttcag ccagctggtg gatgaaatca ccaccgagcc ggattacgaa
     3901 gcagctctgg ctgtactgaa agcataagtt aatatgcctg atggtgttgc accatcaggc
     3961 atattcgcgc ttactcttcg ttcttcttct gactcagacc atattcccgc aacttattgg
     4021 caatcgcggt atgtgaaacg ccgagacgtt ttgccagttt gcgcgtgctg ggataattgc
     4081 gataaagctg ggttaatacc gagcgttcaa aacggctggt gatttcgtcc agcgaacctt
     4141 ccatcgcatc ttcgcccacg gctaccgttg ctgcgtcata atccggcaac aaaatatcct
     4201 gtggacgcag ctcataaccg tccagttgtg tcagtgcgcg atagatagcg ttctttaact
     4261 gccgcacatt tcccggccac gcataacgtg taagtacagt attcaggtca gcggccagtt
     4321 tcggacgcgg cacgccctgc tcgtcggcaa agcgggcgac gaacagctca gttaacggca
     4381 taatgtcctg cggacagtca cgtagcggcg gcagattgag cgtcaacacg ttcagacgat
     4441 aatagagatc ttcacggaac acgccttttt gcaccagttc gaccagattc ttctgcgtag
     4501 cgcaaatcac ccgcacatcg acatgcacct catggtcttc gccaacccga cggaaagtgc
     4561 catcattaag aaaacgcagt aatttcgcct gcatccgtgg tgacatttcc cctatttcat
     4621 ccaacagcac cgaaccaccg ttcgcctgct caaagaaccc tttcttccct tccggagcat
     4681 gaccaaacag ttcactctcg accgcatctt ctggtataga cgcgcaattc agcgccaggt
     4741 aaggtttgcc cgctctgggg cttgcctgat ggcaggcgta ggcaaaaaga tctttacctg
     4801 tacctgtgtc acccgtaatc agcagcggcg cgcttagcat cgccagtttc tgcgcctgtt
     4861 cgacaacgtg cttcattttc gggctgacgg cgacaatttg gctgaaggcg ctgacgtcct
     4921 gggcggcgac attttgcaac tggcggccca tacgaatcgt tgatcgcaac atcaccaccg
     4981 caccggtcag gacgtgttga tcattttcat cctgaagata aacaggcgta atctccatca
     5041 ggaaattctg cccattaata acgacatgct cgttatgcga atcttgcggt tcgctttcca
     5101 gccaacgtaa aaaattaaag ccgttaatca attgtgcggc ggtatggttg cgcaggcgat
     5161 ccaatttttg cccaaaaagc tgacagctcg ccgggttcgc catatccact ttgcttttca
     5221 tatcgacaga gagcacaggt tcaggcaacg cctccagtaa cgcgctcaac gccagatgct
     5281 cacgttcgga aggcatccac gggacagtac gcacatcggt aacacctgca atacggcgta
     5341 tttcggccat caaactgctg aaactctcaa actccagttc agcaaaattg aggtagattc
     5401 gcccaatggg atcaatctca ataccgcgta aatcaatgcc tcttagcacg agtagatcga
     5461 gtaattcgcg ggtcagaccg agtcggtctt cacaaaagac ttccagacgc atgggaacct
     5521 tcacctgaaa aaagaacaat taatatgatg atatgacact atttggatag caggaaggtt
     5581 tctgtcaaca atcattgaca caaagacgga aaaacgggca cttcggcgta aagatatccg
     5641 gtcagcaccc gatattgcat tatttttcgc tgggcgtttt gcctttttgc agagtttctt
     5701 tcacctgacc gataagctga cgacggaaat ccccaaggcg aggtttgtca tcgtcaatcc
     5761 acggcagcgg gcggcaaagc tccatagctt taatcccgag tcgtgccgtc agaagtccag
     5821 caccaattcc ctgagctgcg cgggtagaca aacgagcagc gagatcttgc gacatccagt
     5881 ccatccccac ttcgcgcacc agttcgctgg ctccggcaaa agcgatattc agcaatacca
     5941 gcttaaacag acgcaaacgg ctgtaatacc ccagttcaat gccatacagc gtggcaatgc
     6001 gattaattaa acgcagattg cgccaggcga taaacgccat atcgaccaac gccagcgggc
     6061 tgaccgcaat catcaacgtt gattccgccg ccgaacggct gatttcgcgc cgcgcctggg
     6121 catctaaaac tggctggacc aaatgcgcat acaggctgac cacttcacga tcgttctgcg
     6181 tttcatggat tgaggcatac cagcgttgca gcgccggatg cgactgatca atacccgcct
     6241 gctgcgccag tttttcgcaa aatgcgcgac ctttgcccgt gccgtggcta tgcaataaat
     6301 cacgcgcttc gtcgcgttca tgggcgcgct gtcgcaagcg ccataagcgc cgccactctg
     6361 ttaccacaga acctacgcca gcgccgataa tcaatgcccc agcggcacat ccacccagcg
     6421 ctacccagtc ctgggtttgc caggcattca ttgtccactg tataccctgc ccgacaacgc
     6481 ttgcgccaaa cagagccagc ccgcccatca ccattttgcg ccacaggctg cgtttcggac
     6541 gtaacgctgc gtccattacc gcttcgactt gcccctcttc ttcctgcgct tcgtcgagcg
     6601 tggccggggc aaaattttgc gcctgatttt cgtcaaaggt ctgctgcgcc ctgaattttg
     6661 gattctgttc gacctccagc ggaccgtcga aatcaatacg tggttttaac ggttcggtca
     6721 tcgcaattta tctcctatta aaaattccag cgcggcatca agacgaatat gcggcagcgg
     6781 tttgtcgaca tccatcacct gcgggcgaaa cgcttcaaac tggaaccctt gcttgtccca
     6841 gaacgcctga ccgggcaaac gcgcaggaac ttcaccgggg taaacggtga gaggcgcacc
     6901 atcgctgagc cggttaccgc gcaatgccgg aattttctcg ccgttgacgt caataatgcc
     6961 gctggtggtc gtctgcaccg aggccagccc caggcaatcc atactaatcc tttcaaacgc
     7021 cgcattttgc caggcatcct gaatcagttg ttgcagtaat gaaaccatat tggcgtgctg
     7081 atcgaccgtt acatggtccg ctttcgtcgc ggcaaacagc agtttgtcga taaccggcga
     7141 gaataagcgg cgaaacagcg tgcgctgccc gtagtggaaa ctttgcatca gctgcgtcag
     7201 tgccagacgc atatcattaa atgcctgtgg cccactgttg agaggttgca ggcaatccac
     7261 cagcacaatc tggcggtcaa agcgcagaaa atgattctta tagaacccct tcaccacttt
     7321 ctcgcagtaa taattaaacc gctcgcgcag cattccggca ttggtgtgct tatcggcctg
     7381 cgccagtttg gactcgcccc aggcatcgac atccggccac gggaagaatt gcagcgcggg
     7441 cgcacctgcc atatctcccg gcaagacaaa gcgcccaggc tgaataaagt gcagcccctg
     7501 ctgtttacag tggtggagat aatcggtcca cacggcggca atgtctgcca gccggttttc
     7561 gtcggcaggc gctagcgggt caagtccttc gcacatcatt cgccatttcg ccgaccattc
     7621 gccgcgctga ccattgagta agcccgtcat ctggcgcgac cagcttaagt aatcctgcgc
     7681 cagcatcggc aggtcgagca accattcgcc gggataatcc acaatttcca gatacagcgt
     7741 ggaggtatcc ttaaagtggc gcagcagcga atcgttcgat ttaaaacgta acgccaggcg
     7801 aatttcactg acgccgcgcg taggcgtcgg ccaggcggga ggatcaccat ataactgcgc
     7861 cagtccttca tcataggtga agcgcggaat gccaaagtca cgctggggaa tgcgttttac
     7921 gcccagcaag cgctcttcac gcaccgcgct taacagcggc aaacgtgctc cggcatgaat
     7981 attgagcaac tgattaacca tcgcggtgat aaacgctgtt ttgccgctgc ggctaagtcc
     8041 ggttacagcg aggcgcagat gtctgtcgac accccgattc accagcgcat tgagttcatt
     8101 tttaagtcgc ttcattgctg tcctgtgctg ctctgaagac cagaaaaaaa tcattcgtaa
     8161 ggatatgaat gtgggggcca agccattgat tatcaactgc ccgtgttgtc atcgacgggc
     8221 gaactccata agatgtgcag cattcacgtt cggacggagt tcctgaccta tgtcccctac
     8281 tatttatgat attgccaggg ttgcaggcgt atcaaaatcc accgtatcac gcgtgctgaa
     8341 taagcaaact aatatctccc cggaagcgcg cgaaaaagtg ttacgggcca ttgaagaatt
     8401 acagtatcag ccaaacaagc tggcccgcgc gctgacctct tcgggttttg atgccattat
     8461 ggtgatttct acccgttcga ccaaaactac ggcgggtaat ccgtttttct ctgaagtttt
     8521 gcatgccatc accgccaaag cggaagaaga aggtttcgac gtgatattac aaacgtcgca
     8581 caatccggca gaagatctgc aaaaatgcga aagcaaaatt aaacagaaaa tgattaaagg
     8641 cattattatg ctcagttcgc cagcggatga gtcatttttt gcccaactcg ataaatacga
     8701 tattccggtg gtggtaattg gcaaagttga aggtcaatat gcccatgttt attctgtcga
     8761 taccgataat tttggtgaca gcattgcgtt gaccgatgca ttaattgaaa gtgggcatca
     8821 aaatattgcc tgcctgcatg ctccgcttga tgtccatgtt tcggttgatc gcgttaatgg
     8881 ctataagaaa agtctgtccg cgcataatat tgcagtgcgt gataaatgga ttgttgacgg
     8941 cggttatacc catgaaacag cactgcaagc agcacggcaa ttattaagtc agtcaccgtt
     9001 gcctgaggca gtgtttgcca ctgacagcct gaaattaatg agcatttatc gtgcggcggc
     9061 agagaaaaat attgctattc cgcagcagtt agcggtggtg ggttatagta atgaaacgct
     9121 gtcgtttatt ttaacgcctg caccaggcgg catcgatgtt ccgacgcagg agttaggtca
     9181 acaaagctgc gagttattat tccgcttaat ttcgggaaaa ccgtcaccac agaatattac
     9241 cgttgccacg catatgacgc tgaaataaat accagctacc ttgcctgaat gccggatgcg
     9301 atgctggcgc atcttatccg gcctacattt atagatcgga taaagtactc gcgccgcatc
     9361 cggcaattta gcccattatc agaacgagta atttacgccg acacctgcat aatggaattt
     9421 atcgctgtcg ccttcgtcgt gatcctgcca ctcaaacgcg tattccagcg aaacggaaag
     9481 tccgttctgg aaatcataac cgtaaaataa acccacacgg ttaaaatcat ggccttcacg
     9541 ttcaatatca tcctgccagt cccagttact ccagcgatcc agcccaatgc gcgtatacgg
     9601 cgtcaccgag tggttgccga gcgtcagcgg caaataggcg cgaatttctt gtgtggaaaa
     9661 ctcaccgtta ttgcggctgt cgtccatatt gaagccgcgc tcgagataat agttcactcg
     9721 caaagcaacc gtttcgttga aggtatattg cagacctgtt tccgtttcga cacgggtatc
     9781 agcgtaaccg gtcgtgttca aatcgttggc aaatttatac atcgacaacc aaccgttgaa
     9841 acgtaaatca tcagtcagtt tcacatccca gtctggcgcg attttccagc gctgcatatt
     9901 cgccgtgtct tttcccggtt catcaacgta gtgataacca taattacgga aaccgccggt
     9961 caggccgaaa ctgaaatcat cgctttcgag gaactgataa tgcacctcca gctccgggcg
    10021 atcaaaccac gttccacgtt tacccgcgct ataatctacc ggcccttcct gataataggc
    10081 cagagcaatt ctccacggcc cgttggcggc attaaaatag actgaaggct ccgccaaccc
    10141 atccatatct tcgccataac cctcgacgtt ttctatttcg tacatcgcac cgatattaaa
    10201 gtgccagtcg ttcttttcct cggcctgtgc gcaggccatt cccgcacaca tcaccagtgc
    10261 ggtacagggt aataactttt tcattattgt tccttgtatt atcctagggg aatgccttcc
    10321 ccctgtattt agcgaattgc tatttccgtt tctgcatcaa agaaatgaca tttcgtcata
    10381 tcaaaatgaa tagtgatatt ttctcctgca tgataatcat ttaacgcacc agcacggacc
    10441 actaactcgt gccccccaac cgtggtgtag agcataaatt ccgcaccggt taattctgcc
    10501 acgctaattt tggcggaaat gttattttcc tcttgcgcgt ccggatgaat atcttccggg
    10561 cgtattccca tcacgatggg cttatgcaaa ctttcctgtg ttttcagaac cgctaatttc
    10621 tcttcgagaa tggttaattt aagcgtttcc gtaacgaatt tatcgccatc gatcgtgccg
    10681 cgaataaaat tcattgctgg tgatccaata aatccggcaa caaacatatt cgcgggttgg
    10741 ttataaacgg ttttcggcgc acctacttgc tgaacaatcc cgtctttcat aatcacaatc
    10801 cgcgtcgcca tggtcatcgc ttcggtctga tcgtgggtca cgtagatcat ggtggtgttc
    10861 agtttctgat gcagcttgct gatctctgca cgcatttgta cgcgcagctt ggcatcaagg
    10921 ttagagagcg gttcatccat taaaaacacg cccgcttcgc gcacaatcgc ccgcccgagc
    10981 gccacacgct ggcgttgccc gccggaaagc gcgcccggct tgcgtttcag gtactcacgc
    11041 aggccgagaa tttgcgccgc ccagttcacc cgctcatcaa tcacctcttt ggcgattttt
    11101 tgcatcttca ggccaaacgc catgttgtcg taaaccgtca tatgcggata caacgcgtag
    11161 ttctggaaca ccatcgcgat attgcgtgct ttggctggaa cgtcattcat tcgtttgccg
    11221 tcgatcaaca gatcgccgcc gctgatctcc tcaagcccgg caatcatgcg cagggtggtc
    11281 gatttaccgc agcccgacgg gccgacaaat acgatgaact ctttatcggc aatttccagg
    11341 ttgaagtcct tcaccacatg cacctggtta tcgtagattt tttgaatatg ttgtaacgaa
    11401 agctgagcca tgttgattcc tttgctatac gttttgccag aaggccgata accgcgacca
    11461 ggtcagggag tccgttgagg gcaacagtaa ttgcgccccg gttaagcccg caccgatccc
    11521 caccgagcgc ataccgctgg cgttaatggc gtcaatgccc gcctgcgcat cttcaatgcc
    11581 gatacatgcc tgcggcggca cgcccagccc ggcacaggca gcgagaaaga tttccggatc
    11641 cggtttcgag tttttaagtt gggaagcatc cgcgcagaag gtgaaaaact cgcgcagctc
    11701 cagcgccgct aaaatcgtcg gcgcattcag ggagacggaa gccagcccta ccggaatctg
    11761 ctgtgcacgg agctctgcca gcaaattgcg aataccgggc agcacagcat taaccgtcag
    11821 ctcgcgcagt gagtggacgt aaagcagatt tttgcgatac gccagttgcg cccgctcctg
    11881 cgggttaaag tcgccctctt tgcccccgtg ttgcagaatg cgccgcagag actcatcgcg
    11941 gctgatccct tttagagatt cgttaaactg cgcatcaata ctgatgccaa tttcagcggc
    12001 aatctgctgc cacgcctgga aatgcagatg cgcggtatcg gtgattacac catccagatc
    12061 gaaaattacc ccttgcagtt tcattcatcc tcctgatgcg tggtagcggt cccattgaag
    12121 ggcaaaataa aatcacccaa acagaaaaca gattcttcag ccacggatat aagctgaccg
    12181 ttcaaacgca gtgaaacggg cgcagaagtt cgaatcgcaa tacgctgcgc gtcgagagtg
    12241 acctgtaatt cgcagccctg ccagaacaga gggaaagaca actgttgcca ctgctcaggt
    12301 aacgccggat ttagatgcaa ttcaccgtca cgcacgctca ccccggcaaa accctgaatc
    12361 gcccccagcc agatagcgcc agttgcggca gcatggatac catcatcaca actatgcgga
    12421 tcagcaccaa gatcgatttc agtcccctcg cgccagaact gataactttg ggtcagcagg
    12481 ccacagcgtg cagcaacaat gccgtggatt gctttactta atgacgagtc gtgaatagtg
    12541 cgcggttcat aaaattgcag attggcaaga cacgatgccg atgagaactg ctctggcagc
    12601 atgtaattga gcatcaccac atcagcttgt ttgaggatct gcatctcgtt cacttctgcg
    12661 cgtgaataat ccagcagaat ggtttgcttc cccgccgccg ctttgtattt cgccagatta
    12721 atcgccggct tagtcataaa cgaatcatcc tgcggcaaaa caccgtcggg ctgaatttct
    12781 ggcagccgca gttctttaag gaacatttcg gcacgatgaa taaacgcgtc gtcactacag
    12841 ccaaactggc gggcaatact cagcgcctgc tggacgttgt aatacaccat atagctggtg
    12901 aaggcgttat tattgacatg ttcggtatat tcgtctggcc caataacatc atgaatttcc
    12961 agacggtcgt taaccctcac cgcgcggcta atccagaact ttgcagtttc cagaagtagc
    13021 gccatgcctt catgcgcaat gaaactttca tcccccgtgg tctgccagta ttgaataacc
    13081 gcccaggcga tatcggccac cagatgatgt tccgcctgcg ccgaggccac tttttgccgc
    13141 agcccggtgc gaatgttaat ggcggcaaat tccggcgtct cttcttcgcc gctgcgcgcg
    13201 ctttcccacg gaaatagcgc gccctgccag ccgttgcgtc gcgctttctc ctgcgcgcct
    13261 ggcaagttgt gccagcgata acgcagtaaa cttcgggcaa ccgtcggatc gctaaacaga
    13321 tggaacggca gcaaaaatac ttctgtatcc cagaaaacgt ggcctttgta gccttcgccg
    13381 gttaagcctt ttgccgcaat actgctgcgc tcgccgtgag ccggcgtcat gatgcgcaga
    13441 tgataaagcg cataatctaa cgcttgctga tcgtgcgcat cgccgccatt taccgtgata
    13501 cgacgtttct gccaccattg acgccagttt tctgttgatg ctgcaagatg ttggtcgtaa
    13561 ctctgttgcg cgcacat
//
LOCUS       DADWTH010000105        13527 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.106, whole genome shotgun sequence.
ACCESSION   DADWTH010000105 DADWTH010000000
VERSION     DADWTH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.106"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..895)
                     /locus_tag="JNY02_003246"
                     /pseudo
     CDS             complement(1..895)
                     /locus_tag="JNY02_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1117..1917
                     /locus_tag="JNY02_003247"
     CDS             1117..1917
                     /locus_tag="JNY02_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBB9434687.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1995..2765
                     /locus_tag="JNY02_003248"
     CDS             1995..2765
                     /locus_tag="JNY02_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB9434688.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2813..4171)
                     /gene="mltD"
                     /locus_tag="JNY02_003249"
     CDS             complement(2813..4171)
                     /gene="mltD"
                     /locus_tag="JNY02_003249"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBB9434689.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4243..4998)
                     /gene="gloB"
                     /locus_tag="JNY02_003250"
     CDS             complement(4243..4998)
                     /gene="gloB"
                     /locus_tag="JNY02_003250"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBB9434690.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5032..5754
                     /locus_tag="JNY02_003251"
     CDS             5032..5754
                     /locus_tag="JNY02_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB9434691.1"
                     /translation="MKPARVPQTVVAPDCWGDLPRGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5751..6218)
                     /gene="rnhA"
                     /locus_tag="JNY02_003252"
     CDS             complement(5751..6218)
                     /gene="rnhA"
                     /locus_tag="JNY02_003252"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBB9434692.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6283..7014
                     /gene="dnaQ"
                     /locus_tag="JNY02_003253"
     CDS             6283..7014
                     /gene="dnaQ"
                     /locus_tag="JNY02_003253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBB9434693.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7147..7223
                     /locus_tag="JNY02_003254"
     tRNA            7147..7223
                     /locus_tag="JNY02_003254"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7181..7183,aa:Asp,seq:gtc)
     gene            complement(7354..8400)
                     /locus_tag="JNY02_003255"
     CDS             complement(7354..8400)
                     /locus_tag="JNY02_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBB9434694.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8411..9406)
                     /gene="tssG"
                     /locus_tag="JNY02_003256"
                     /pseudo
     CDS             complement(8411..9406)
                     /gene="tssG"
                     /locus_tag="JNY02_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9403..11286)
                     /gene="tssF"
                     /locus_tag="JNY02_003257"
                     /pseudo
     CDS             complement(9403..11286)
                     /gene="tssF"
                     /locus_tag="JNY02_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            complement(11302..11796)
                     /gene="tssE"
                     /locus_tag="JNY02_003258"
     CDS             complement(11302..11796)
                     /gene="tssE"
                     /locus_tag="JNY02_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBB9434695.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11793..12623)
                     /locus_tag="JNY02_003259"
     CDS             complement(11793..12623)
                     /locus_tag="JNY02_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HBB9434696.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12610..12987)
                     /gene="tagK"
                     /locus_tag="JNY02_003260"
     CDS             complement(12610..12987)
                     /gene="tagK"
                     /locus_tag="JNY02_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HBB9434697.1"
                     /translation="MLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLVTEAETLQDMVT
                     GGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNTVLPDLTRKEHR
                     IIGIDNHYRINPAQHGEIYHDKQ"
     gene            complement(13224..13394)
                     /locus_tag="JNY02_003261"
     CDS             complement(13224..13394)
                     /locus_tag="JNY02_003261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HBB9434698.1"
                     /translation="MNDDVKIALTLTRHEEAWWIINQSTEYCCTVNDQIVEPHHRMRL
                     NEGDLIEWGLSS"
BASE COUNT         3094 a         3522 c         3612 g         3299 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaatta tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta atagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaaaattg ttccgctgcc cggctaaagc
      841 tgccgctttc gacgaccgaa acaaaaatgg cgagttcttc cgacgtggct ttcatttttg
      901 ctcctatttt gaatttattt gtcattaaac catcaggatg cgtgatatgt catccgattt
      961 aatgttctca ataatgagca aatttctgac cggtgtaagc acttgcttac ataacaatat
     1021 acaattgctc gttgaaagag tgagctaaaa tccctataac agtagaaccc tcccgagtgc
     1081 ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc gaaaaaacac ctatgccatg
     1141 cgctatgttg ccggacaacc tgcggaaagg atcttaccgc cggggtcttt tgcgagcatc
     1201 ggccaggcat taccacctgg agaaccgtta agtaccgaag agcgtattcg gatcctggtg
     1261 tggaacatat acaaacagca acgcgctgaa tggttgtcgg tattaaagaa ctacggcaaa
     1321 gatgcacatc tggtgttatt gcaggaagcg cagacaacgc cagagttagt acagtttgcg
     1381 accgctaact atcttgccgc cgatcaggtg cccgctttcg tgctgccaca acatccttcc
     1441 ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct gcccgttacg tgaacgagaa
     1501 cccattttgc gtctggcgaa gtcggcactg gtgacggtct atccattacc tgacacccgc
     1561 ctgttgatgg tggttaatat acacgccgtc aacttcagtc tgggcgtgga tgtctatagt
     1621 aagcagttac ttcctattgg tgatcagata gctcaccaca gcggcccggt cattatggcg
     1681 ggagatttca atgcctggag ccgtagaagg atgaacgcgt tatatcgctt tgcgcgggaa
     1741 atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc gtcgggcgtt tggccgcccg
     1801 ctcgattttg ttttctaccg tggtctgaac gtcagcgaag cttctgtatt ggttacgcgc
     1861 gcttccgatc acaatccgct actcgttgaa ttcagtcccg gcaagcctga taaataaggt
     1921 atgtcaggtc tgccacaggg cagaccaacg tttggcgctg tccttttata ctcaaacaac
     1981 aaaaggacag cgcaatgaca acacaatccc accatgacca cgtagaaaag cagtttagct
     2041 ctcaagcctg cgaatatctg accagtaccg tacatgcatc cggacgagat ttacagcgcc
     2101 tggcggtgcg tctggctgat tatcctggtg caagtgtact tgatatgggc tgcggagcag
     2161 ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt ggtggcgtat gacttatctg
     2221 cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg gcaactgaaa aatatcacca
     2281 cccgccaggg atatgccgaa agtctgccat ttgccgataa cgcatttgat attgttatca
     2341 gccgttattc tgcccatcac tggcatgatg ttggtgcagc actgcgtgaa gtgaaccgga
     2401 tattgaaacc tggcggtagg atgattgtga tggacgtaat gtctccgggt catccagtgc
     2461 gcgacatctg gttacagacg gtagaagcat tacgcgatac ctctcacgta cgaaactacg
     2521 ccagcggtga gtggttgagg ttaatcaatg aagccaatct gatagttgat aatttaatta
     2581 cagataagtt accgctggaa ttttgttcat gggtcgcgag aatgcgtacg ccagaagcgt
     2641 tagtagacgc tattcgcatt taccaacaga gcgcatcgac agaggtgaga acgtattttg
     2701 ccttgcagaa tgacggctct ttcaccagtg atatcatcat ggtagaagca cataaagcgg
     2761 cataaataaa aaaggcaccg ggggaatcgg taccttttta ttatctggtt tgtcaggaat
     2821 ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc gcctggttgc agattcgcag
     2881 tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc gtggcgttta gcaatgcttg
     2941 aaagcgaatc gcctttgcgc acacgatacg taatgctatc gctgttgttt gccagtcgct
     3001 gtgcgctact gcctgcacca atcgtcaaac tttggcctgg cttcagctta gatccgcgca
     3061 gtttgttcca ctgctgcaaa tctttggtgc ttacgccgag acgtgaagcg atgcttgaaa
     3121 gcgtgtcgcc agagcgtacg gtgtaaacac ggctgttaag cggcgtattg tcggcaacca
     3181 gcgtcgactg tacagcagca atttcgcctg aagcgagaga ttcacgcagt tgatctgcat
     3241 gcttctttgg caccatcacg tactgcggcc cacttgcgcc cagcgtggag cctttcacgc
     3301 cagcgttgaa tgtcttcagc ttgctgacgg aaatccccgc catatctgca accttcgcca
     3361 tttcaaccgg gctactcagg tgcacacgcg ccagagcacg gctttcatcg gtcgttggca
     3421 gacgtacgcc ataacgcttg ctgtttttga gaatatcact caatgccagc attttaggca
     3481 cgtactgctt cgtttcctgc ggcaacggta acgaccagaa gtccgtggat ttcccacgcg
     3541 ctttgttcgt tttaattgcc ttcatgaccc gaccttcgcc gctgttataa gccgctacgg
     3601 tcagaagcca gtcgccgtca aacattttgt tcagacgctg catcatattc agcgcggcag
     3661 ttgttgaagc aacaacatcg cgacgcgcgt cataattgcg ggtctgtttc aaaccataat
     3721 tgcgccccgt gctcggaatg atctgccaga tgcctgcggc attggcgcca gacgttgcgt
     3781 gaggatcaaa agcgctctcc actatgggta gtagtaccag ttccataggc atgttacgtt
     3841 ttttaacttg ccctgctatc cagtacatat acggctctgc ccgtaaagtt acatcgtgga
     3901 gatagctctt attgcgtaaa tatttctgtt tctgttcgcg aatccggtca ttttccggaa
     3961 ttcccatctt tagctcgtcg ccaatgaaag cccacaagtc accatctggc gcgatagacg
     4021 tcccatcgtc catccatcgt gcctgacttg taaactttgc tgcttcccct tgaccagctg
     4081 cagaaaggct ctgtgcgtgc tgttgaacgt tgccggtact ctggcaaccc acgagcagga
     4141 cagaggcgag taatatcgct tttgccttca tgtgtgtgtc aatagttgct taaaagacga
     4201 ccgatcataa cggcgaacgg agccgatgac aagaaagttt tatcagaacc tatctttctt
     4261 tgaccttaac catgcaaaac gctcttcagg ttgttgcaat aatgtttctt cattaattac
     4321 attaattaaa tcaatatctt ccgttcttaa aaaaacatta atttgccgct catttttcag
     4381 aattacgggt agtgttattt gattttttgc ccgtaactcc ttaactttac gataataatc
     4441 atttatggac aaatcgtgcg gaaaaatact caaagcaaac ttcatatttg ataacgtata
     4501 ttcatgagca caacatacca atgtatcgtc aggtaacgca cttagctttt taagtgattg
     4561 atacatttgt aaagctgtcc cttcaaacaa ccgaccacac ccaccagaaa acagcgtgtc
     4621 gccgcaaaat agataaggtt tactgaagta acagatatgt cctaaagtgt gacccggtgt
     4681 ggtaattaca ctaaattcat gccctaaaac gaaggcagtt tcgccatctt tgactacctg
     4741 tgttgttccc ttatcttgtg tctcttgtgg accatacacc acaatttgcg gaaacttttc
     4801 caccagttct tttacgccgc caacgtgatc gtggtgatgg tgggttagaa atatggcctc
     4861 cggttgccag ttattggcgg taatggcgtt taatactggc tctgcgtctc cgggatcgac
     4921 aatcaggcag cgacctgcct catcattcaa aacccagatg taattgtcat caaaggcggg
     4981 aatactgtta agattcatag attacctctc agtgtaaaac ggaaggttgt gatgaaaccg
     5041 gcaagagtcc ctcaaactgt cgtggctcct gattgctggg gcgatttgcc caggggagag
     5101 ctttatcgca aggcgctgga gcgccagctc aacccgtggt tcactaaaat gtatggtttt
     5161 catctgctta agattggcaa tttaagcgca gaaatcaatt gcgaagcgtg cgcggtttct
     5221 catcaagtga atgtttctgc gcaaggaatg cccgtccagg tgcaggcgga tccacttcat
     5281 cttccttttg ccgataaatc cgttgatatt tgtctactgg cacatacctt gccgtggtgc
     5341 accgatccgc atcgtttatt acgtgaagcc gatcgggtac tgattgatga tggctggctg
     5401 gtgattagtg gcttcaaccc catcagttta atgggattac gcaaacttgt gccggtattg
     5461 cgcaaaacct cgccctataa tagccggatg tttactctga tgcggcaact ggactggctc
     5521 tctttgttga attttgaagt gctgcacgcc agccgtttcc acgttctccc gtggaacaaa
     5581 cacggaggaa aactattgaa tgcgcatatt cctgcgcttg gttgcttaca acttattgtt
     5641 gcccggaaac ggactattcc tttaacgcta aatccgatga aacagagtaa aaacaagcca
     5701 cgaattcgcc aggcggttgg agccacccgg caatgtcgta aaccacaggc ttaaacttcg
     5761 acttggtagc ccgtatcttc cagtgtggga ttcatcgccg cggcacgagc cagttcatca
     5821 cagcgttcgt tttccgggtg tccggcatgg cctttaaccc attcccattt gatttgatgc
     5881 tgccccaatg cagcatcaag acgttgccag agatcgacat tttttactgg ttttttgtct
     5941 gctgttttcc agccacgttt tttccagtta tggatccact gggtgatacc ctggcggaca
     6001 tactggctgt cggtactcaa aatgacttcg caatgttctt ttaacgcctc cagcgcgaca
     6061 atagcggcca tcaactccat acggttgttg gtggtgcggg tgtagcctgc gctaaaggtt
     6121 ttctcgcgtc cgcgatagcg taaaatagcg ccgtaacccc caggtccagg attacccaga
     6181 cacgaaccat cggtgaaaat ttctacctgt ttaagcatct ctggtagact tcctgtaatt
     6241 gaatcgaact gtaaaacgac aagtctgaca taaatgaccg ctatgagcac tgcaattaca
     6301 cgccagatcg ttctcgatac cgaaaccacc ggtatgaacc agattggtgc acactatgaa
     6361 ggccacaaga tcattgagat tggtgctgtt gaagtagtga accgtcgcct gacgggcaat
     6421 aacttccatg tttatctcaa acccgatcgg ctggtggatc cggaagcctt tggcgtacat
     6481 ggtattgctg atgaattttt gctcgataag cccacgtttg ccgaagtagc cgatgagttt
     6541 atggactata tccgcggcgc ggagttggtg atccataacg cagcgttcga tataggcttt
     6601 atggactacg agttttcgtt gcttaagcgc gatattccga agaccaatac cttctgtaag
     6661 gtcaccgata gccttgcggt ggcgaggaaa atgtttcccg gtaaacgcaa cagcctcgat
     6721 gcgctatgtg cccgctacga aatagataac agtaaacgaa cgctgcacgg ggcattactc
     6781 gatgcccaga tccttgcgga agtttatctg gcgatgaccg gtggtcaaac gtcgatggct
     6841 tttgcgatgg aaggagagac acagcagcaa caaggtgaag caacaattca gcgcattgta
     6901 cgtcaggcaa gtaagttacg cgttgttttt gcgacagatg aagagctggc agcacatgaa
     6961 gcccgtctcg atctggtgca gaagaaaggc ggaagctgcc tctggcgggc ataaatacat
     7021 gtaaaaggcg ctaaaaatag cgacttgggc gatttttgca gcaaacgatt caaaagatga
     7081 gaaaaatcgt tgacgaaggt cgaggcaatc cgtaatattc gcctcgttcc caacggaaca
     7141 caacgcggag cggtagttca gtcggttaga atacctgcct gtcacgcagg ggggcgcggg
     7201 ttcgagtccc gtccgttccg ccaatattca gaaagcccga atcagtaatg attcgggctt
     7261 tcgtcgtttt ggtgaagtga gaatttttca ctccgaagta aaacggatat gatttaaaaa
     7321 atggccagac attataaatt ctggccactt ctgttactcc agcgcatccc atttctccag
     7381 cagttctact ggaatttcat ttattacctc cgagacgcgt tttccgacca ggcgttctgc
     7441 ctggcgcagc aaaggaatgg tcgggctgct cggttcgctg tgctcaaacc agcgacggat
     7501 gacacgcagg cgttccagcg cgtcgtttcg gtcgcggatc tgccggggtt ctgcgccggt
     7561 catcggtgat atttcctccg cgatatgcac cggtagcgca atctgctctg cctgcatctg
     7621 ttcagtatga actatggtgg cgttttccgg ctgctcagcc tgtggttgtg gcaggatctc
     7681 cggcgtggtg ctctgcactg ctcccggtaa cagggccagc agttgcgtca gacgggagaa
     7741 gtccggtgcc tggtcattca gcgtttcacg cgcatggcgt tgcagccgct cagtggtctc
     7801 tgctgcctgg cgaaaggcat ccagcggcag tgtgccgcgc gcttccaggt ctgccagttg
     7861 ctggcgtacc gattctggtg ccagcgcatc cgccgggcgt aatgcagata gcgaacgctc
     7921 cacatcccgc acctgaaggc gcatggcagc attatttgac agtgtaatgc cacgaatatc
     7981 cgccattaca ccttcgtggt ccagcaacgc cgccagcgcg ttactgcgtg ccaccgcagc
     8041 atcttcggcg gtgatatctt cagtcgcgag cagttgagga tggatggcgt ccgggtacgt
     8101 ggagcacaac gtttccagca gcgtgagcgc ctccgacagt cccctggcac cggcctgctg
     8161 gatacggcag cgcagcagga caaccagaat gcggatatct ttgctgcgca tcagcagacg
     8221 atgggcgtcc cgctcaagct ccgcccagtt aatgttttcc ggcgtactga caaaatcgcc
     8281 atattgcgct tcttcacgcg gtgcggcgcg ggtgaacagc agcaggtatt ccggatcgta
     8341 ctccagatcc gggccgcagg ggcattccgc cgaaattggg tcagacagga atatttccat
     8401 ttaattatcc tcagtgcgcc aggtagtgtt ctggttcgaa aatcatgccc gtcaccgggt
     8461 tgtcgtgcat ggctttaccc gcccatgcag tccaacccag ttggtacgga ccaccggtaa
     8521 cagccggagg ggcggcgtgg ggttttagct gtagctcaat ttcccagcag tattcataac
     8581 cactaaaggc gcggaccagt tctgtcagga cgggcaggtt attgctgcca ggcagaaaac
     8641 gtcggtaatc ctccagcgtc agcgggccga taaccagccg gaacttatac tgcatgtccg
     8701 gcacggcctg gccgatgagt gcaccattgc caataacgga agagggggca ggcatcccca
     8761 gccgggtgat ctcatcgtcc gccacggcta tccagtgcag gacatactcc tgtatccgga
     8821 aaggtacacc gaaatagtgc gccagcgtgg tggccagacc gtccggattg cgggactcac
     8881 gcaccagatg ggccgatgcc gccagacgag cgtgatctgg caacgggctg tcagcgctct
     8941 cccgcaaatc ctgcccgctg aggctggcga cataaaaggc aaagcggtca tgttgcggtt
     9001 tatccagacc gcccccagcg gactgggcgc tgcgccatgc ctggtaaaac tgcgtcagcc
     9061 agcggtgatg aaaaagatca gaaaaacggg tcagcgtcgg atcgcgtcgg ctttcagtcc
     9121 ggttacgcgc cagttcggtg tagtgcagcg gcagtggacc gttcggcccc cacagcccga
     9181 ggctgtacag actgatgtgc aggcgccctt cctgccagcc cacactggcg atttctcgtg
     9241 gcgcaaaggt cagcgcgggc gtttgtccga ggcgaaattt ttccatccgt ggttgctagg
     9301 tgttgcccgc cggaacggta cacaactgca catccacgcg gcgcatcagg ttcagaaaac
     9361 cgtaatgcca ggggggttcc tgcgcctgag tgaaatcgtt gctcatacgc ttcccctctg
     9421 tcccgccctg acaggccagg tcataatttt gccgcgctgc atgctgtgca gcgtcatctg
     9481 cgagaaggta ttaatggaaa catggcgggc gatatagtgt tccagcacca ggccgaacag
     9541 atacggactg atgccggaaa aaccttcttc atccaccgtc agttcacaac tgacgccacg
     9601 cccatagacc agtagtccgg aaccgggcag acggcgggtg accggggtcg ttttacaacc
     9661 aatcaggctg cgcacctggc gcgattgcgg actgtcatga gcaggaataa acaggttcag
     9721 cagatctcgc aacgcctggc cgccggtacg gtgatccaga tcggccagcg gcagataatt
     9781 aaaagataac tggcggatca accgccatgc catctcacgt tcagccagcg ggggctgcgg
     9841 cgagcgtggc gggcggataa gccctacgcc cgccaccggg atggctgcat ccacggttag
     9901 atcgtcccgg ccattgcggg caataagaca gggcagatcg cggttagtga ccatggccgt
     9961 gatggtgatg tggcgcagat tttccgggta cggcgcttca tactggtcaa ccagcgacag
    10021 gaacacttcc gagcccgtat agggggtacg ggtgccgtaa cggcgggcgt tttctgataa
    10081 gcggcggggc tcccggcgca gggaaaaata acgtccgtga ttgccttcat cattattgcg
    10141 ggtgtggtag agcgggcgaa aggccatctt ccgtgtggtg tcggtttcca gcccttccac
    10201 ttcctggacc gagaacacct cataatccag cggatgggtt ctgtccacca ccagatgctg
    10261 ttccgtcgtg ctgtgcgtga catcgatccg ggcggtgacg cggggaaaca ggttgataac
    10321 cggggtacag aacaggctga actgtgccgc atttgtctgg tggatcagtc agtccggcgg
    10381 caggcggttc agcaggataa caatctccgc cacgccaccg tcaattttct gtaatccggc
    10441 agacaggcct gtcggggtga aaaaatagaa gcgttccgga caggcaaaat attcatgcag
    10501 caggttatga ccgtgaaaca cgttccacgc cagcggcagc agcccctgat ccggctccag
    10561 gccttcgtac ataacgggct gttgcagatt cacatccagc gcgccgtcaa aatgccccgg
    10621 aatgccagcc agggtcgcga tggcgctggt atgtagaagt tctaacaggt gtgaggccgt
    10681 acgctcctcg ccgcagagat aaaacggcag cctgtccagt ccggcaagct gactgaacgt
    10741 tagttcacca aaggtgcgca gggtaatacg cagcgccccg gcgacgtgaa tgttggccgg
    10801 caggtagcgg tgcagggcag gcatatccgg tggcgcggcg gtcagtcgcg cctcttcgat
    10861 ggcgagcggc cacagcgtta catcctggct gctgcgaaac tggcaggcag tgctttcgcc
    10921 ttccggaatg gcggagaaaa aggctgtgtc gcgcgggacg gtaaatcctt tcgccagatc
    10981 gccttcttcc gtatcgggat gcaactgcgc cacgctcatt gagggcgtgg gtgtgacgta
    11041 attggggctg accacttcca gcgggcgctg ggtgaaacgg gggaattcag cgtcaatttt
    11101 tagctgcgtg cgggcggtca ggaagctgaa ggcctcgacc attcgctctg catacggatc
    11161 ggcaatatcc gttccctgca tgcccagccg ggcggcgact ttgggatgac gggcggcaaa
    11221 ttcggctccc gtttcccgca gatagctcag ttcgcggttg tagtatccca gtagccgtgg
    11281 atccatcggt ctctctctga attaaaaaaa cgtaatgcga ctctgctcca tatccagcgc
    11341 gctacgtacc cggaatgcag tcggatacgg ctgcgtgagg atctcgccgc gaatttcaaa
    11401 ctgtaatgtg ttatggccac tctggcggcg ctcatcctga agtgggatga tttgcagggt
    11461 ggacgctttc agccggggtt caaaccgggt gatggttcgc aaaatggccc tgcggatatc
    11521 gtcccacttg tgctcataca gaaagctgcc cgccagtggc ggcaggccgt agttaagtac
    11581 ggacgctgcc gcgtacggat aacgtgcagc atcaatatca tcttcatggc tgacggtgtt
    11641 gagcagaaat gacaaatccc ggcggatgat ctccttaatc tgaaccgggc tgacgctgat
    11701 accgtggtcg cgtttctggt ttggggcgtc gtcacacagg cggtcaaaca gcgtgggcag
    11761 caggtgattg cctggacgct ggcgggaagc gctcacgcgt cgcttccttc agcggtgtta
    11821 aaggtacagg tggacaaatc cagcaggctg atatcgccgt gaccggtgag ccacaccctc
    11881 tgacccagcg cctgtaccag tgtctctccc ggaccgtcct gccagaccgt ttcccggcac
    11941 agacgcaggg agtcggcagc cttttcagag ccgctgtagc ggggaaacag ccaggcactg
    12001 tgtgtatcgc cattgttcag ggtgacgttg acgggtttcc agatcaaatc gatcagatgc
    12061 gcaggccgcg gcgatttcag cgaacagatt tgtgaaaacg gcagccagat atatgctccg
    12121 cccgcgatca gttcgagtac cggaccggta cgggagtcgc tgtcgctaat ccagtcaaat
    12181 gcgccgccat tccactgccc ggaagtgtct gttatggcct caagcgcgcg gctgcggtga
    12241 gcatcagctt cctgcgcttc accacgggcg ttacaggcga gggtggtcag cagatcctcc
    12301 atccaggcgg gcggcgggag aataacaccg ggccgctgtt cgccctgaaa gcaggcgtgg
    12361 cggtatatct cacagcggat cagctcgcca aatacctgcg cttcgcgagt ataatttgcg
    12421 tccatccggg cgcatagctg gatctgttgc agtgcccgcg accagtcgcc tgccacgcac
    12481 aataactgaa acaggctgtg gcggcgggag gcctgcgctg ggtgcatcct gacctgttgc
    12541 tctgccatct gaatgccctc caccacggaa taatcccgca gcagcgccga caaggtaatc
    12601 ggtagctcgt tattgtttgt catgatatat ctctccatgt tgtgccgggt ttatccggta
    12661 atggttgtca atgccaatga tgcgatgctc tttccgtgtc agatctggca gtacggtgtt
    12721 atgcgctgtt tccggggcgt attccgggga aagtaaatgc aggatatcag ggggagtctt
    12781 ttcgacaggc cagatagttt catcttcgcc agtggcatcc agtacattga gaatagcgtc
    12841 aatgcttaac ccaccagtga ccatatcctg caatgtctcg gcttcggtga ccaggtcacg
    12901 caatgaatcc agatcctgcg aggagggctc ttcgttaaaa ggaaaaggat cagcatggcg
    12961 attaagatct tgttcgggag ataacaacgc ctccggtatt cctgataaag tcgggtcagg
    13021 gcatttcctt tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg
    13081 aatggatttt gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt
    13141 attgcttccg acgtgatggc agtctgcgat atcgttttta ctgtgctcag gtccggagat
    13201 gatggattgc catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca
    13261 ttcagacgca tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg
    13321 gtagactgat tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt
    13381 acatcatcat tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt
    13441 gttccttcag tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt
    13501 ccccacgtaa tatttggttt cattgtg
//
LOCUS       DADWTH010000106        13174 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.107, whole genome shotgun sequence.
ACCESSION   DADWTH010000106 DADWTH010000000
VERSION     DADWTH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.107"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            19..255
                     /locus_tag="JNY02_003262"
     CDS             19..255
                     /locus_tag="JNY02_003262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434699.1"
                     /translation="METHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLVAMWEVR
                     GNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(321..553)
                     /locus_tag="JNY02_003263"
                     /pseudo
     CDS             complement(321..553)
                     /locus_tag="JNY02_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(663..1433)
                     /gene="yafV"
                     /locus_tag="JNY02_003264"
     CDS             complement(663..1433)
                     /gene="yafV"
                     /locus_tag="JNY02_003264"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBB9434700.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1614..2060
                     /gene="ivy"
                     /locus_tag="JNY02_003265"
     CDS             1614..2060
                     /gene="ivy"
                     /locus_tag="JNY02_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBB9434701.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2103..4547)
                     /gene="fadE"
                     /locus_tag="JNY02_003266"
     CDS             complement(2103..4547)
                     /gene="fadE"
                     /locus_tag="JNY02_003266"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBB9434702.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4786..5364
                     /gene="lpcA"
                     /locus_tag="JNY02_003267"
     CDS             4786..5364
                     /gene="lpcA"
                     /locus_tag="JNY02_003267"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBB9434703.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5570..6337
                     /locus_tag="JNY02_003268"
     CDS             5570..6337
                     /locus_tag="JNY02_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBB9434704.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6308..7048)
                     /locus_tag="JNY02_003269"
     CDS             complement(6308..7048)
                     /locus_tag="JNY02_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBB9434705.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7432..7953
                     /locus_tag="JNY02_003270"
                     /pseudo
     CDS             7432..7953
                     /locus_tag="JNY02_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8129..8626
                     /gene="rayT"
                     /locus_tag="JNY02_003271"
     CDS             8129..8626
                     /gene="rayT"
                     /locus_tag="JNY02_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBB9434706.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(8843..10555)
                     /locus_tag="JNY02_003272"
                     /pseudo
     CDS             complement(8843..10555)
                     /locus_tag="JNY02_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10527..11312
                     /gene="lafU"
                     /locus_tag="JNY02_003273"
     CDS             10527..11312
                     /gene="lafU"
                     /locus_tag="JNY02_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBB9434707.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11383..12438
                     /gene="dinB"
                     /locus_tag="JNY02_003274"
     CDS             11383..12438
                     /gene="dinB"
                     /locus_tag="JNY02_003274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBB9434708.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12490..12783
                     /gene="yafN"
                     /locus_tag="JNY02_003275"
     CDS             12490..12783
                     /gene="yafN"
                     /locus_tag="JNY02_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBB9434709.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12786..>13174
                     /gene="yafO"
                     /locus_tag="JNY02_003276"
     CDS             12786..>13174
                     /gene="yafO"
                     /locus_tag="JNY02_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBB9434710.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
BASE COUNT         3186 a         3451 c         3396 g         3141 t
ORIGIN      
        1 aagggatata aagtaacaat ggaaactcat gcattatctg acgattgttt agattgtgta
       61 tatgattggt atgcggaaaa aaatggacaa ttttttagtg caacgagtcc agaagagtta
      121 cttggattag ttgcaatgtg ggaagttcgt ggtaatgact ggagagctaa tttgaatgag
      181 ataaatactt atcaaaaagc catagaagaa tccccgtttt ttgataaaaa cggcaatgaa
      241 atagagtctg actaaatttt attgatatag caacgataat aaccggaaat ttcttccaga
      301 tctttccggc tttttattat ttaactttcg ttcagcacac taaacatccc gtctcgcatc
      361 cccttaactg actgtttaac ctgataaagt tccctgcgca gttcctgtac ttcgttgtta
      421 tcccaagcag gatcaaacat ccctccgaga tcttcacggt aacgcccgtg tcgatctcaa
      481 atcccgcttt tctcagccat ttggcgctga ttttgatcac cggcgtgtcg ctgcggttgg
      541 gcagataacc cacggtacag cgacgctgtg ttttggaaat ggtgacttct ggcgtaatat
      601 tgtgtttaag ttaggggctt tcgtaatcta gcccagcccc cggccctaaa cacaaccccc
      661 actcaccaca acctaaactc atccgcatcc cgccatgccg gaaacttttc tctatattcc
      721 cgcaatgcca ccatcgacag ctccgcatca atacgcgttg ccttatgcgc gtcggcggta
      781 gcgataatct cgccttgcgg attaatcacc cggctgtcac cgcgataatg gcagccgttg
      841 ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat cgcgcgggcc
      901 gtcagcaatg cctgccagtg cagagagcgc ggagctggcc agttggcgac gtacagggcg
      961 aggtcataat cgttgagatt gcgcgaccac actggaaaac gtaagtcgta gcacaccagc
     1021 ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc tttataatgc
     1081 agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac cgtgccgccc
     1141 ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc aacactgcct
     1201 gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agttcactac gtcatcttgt
     1261 gcaagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctc cggtagaacg
     1321 atcacatcgc gcccggtaat accttccagt tgacgatcaa aatggcgcag gttggcagga
     1381 ccatccaccc acaccaacgg ttgctgcaaa agcgtaatct tcaaactagg cacggtgtac
     1441 aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt gccttagcgc
     1501 agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat tatcgtgtta
     1561 tcgccaggct ttaggaggtt aataacatgg gcaggataag ctcgggagga atgatgttta
     1621 aggcaataac gacagtcgcc gcactggtca tcgccaccag tgcaatggcg caggatgatt
     1681 taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcgtttaat cagatggtgc
     1741 aagggcataa gctacctgca tgggtgatga aaggcggtac ttatactccc gcacaaaccg
     1801 taacgttggg agatgagacg tatcaggtga tgagcgcgtg caaaccgcat gactgtggct
     1861 cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg ctgttctcgg
     1921 ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac gatgcgcttt
     1981 cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa aaccatccgg
     2041 atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc tccgttattc
     2101 aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt cgtcgccagc
     2161 tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc cgctttcacc
     2221 agaatagcgg cttcatcttt atcaatcagc cccttcgtca gcgcgttgtg cgccagttca
     2281 tccagacggg taaatggcag atttttaccc agctctttac agatccgctg atgaatcgag
     2341 tcggcggcaa tcacatccac caacgcctct tccagtaaac caaccgggtt atgctcgctt
     2401 ggcgtcaggt actgaccgcg accaatacgt gaacgagtgg cgttcggcac ttgtaaaatc
     2461 ttcgccactt tatgatccag cttgtcagac ggtgccagat aatgacgtcc ggtcgggaag
     2521 atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag taaatcgtcc
     2581 atcgcctgtt ctgcctgata cagcgcatcc tgtacgcccc agtgcaccag cggcagatcg
     2641 gcttcattac ggccttcgtc gtcataacgc ttcagaacgg cagaggcgag gtaaagctgg
     2701 cttaaaatat cccccagacg tgccgagata cgctcacgac gtttcaggct gccgcccagc
     2761 agtgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt caggtgctga
     2821 tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg cgtcaggccc
     2881 agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa caacagttta
     2941 tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac gtacggatgg
     3001 caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt agccccttca
     3061 acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct ttgcccgagc
     3121 ataatgcctt taccgccggt aatatccatc gcatcaataa tcgactgctg cccgcggtgg
     3181 gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag cataatgccg
     3241 taggtaatca gcgatgccgc agcatccatt acataagcgt tcccggcaat acgcgccagc
     3301 ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg aatgtgagaa
     3361 tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga aggcagggtg
     3421 atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc ggccattttc
     3481 ggcccgccga ttatgtaatc gatcggcacg aagacatctt taccgcgcgt cggtccgttc
     3541 tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg cgtggtggtt
     3601 gggatcagcg cacaggtaat gcctaaatct tctgcaccgc cgagtaattt ttccgggtcg
     3661 gagagtttaa acgccagccc aagcacggtc gcaatcggtg ccagcgtaat gtagcgtttg
     3721 ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat gcagacaatc
     3781 ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggctggtcag tgcaaagcag
     3841 gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc gtcagtgccg
     3901 taatgttgca acagttcgcc cgggcctaat gagtttggca cgccgacggt aatcgccagg
     3961 atcccgctca cgccagagag tttttgcagc acacgagact gggcataagc tgagaactcc
     4021 agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt aaggtacgcc
     4081 cacaactccg gcggcagatc cgccagctca tgagtaatct ggaaatcatt cgccatccgg
     4141 caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cagcggtcag gcgcggctgc
     4201 ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc ctcccaccag
     4261 gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat caccttacgg
     4321 aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa gttaaatggc
     4381 acgaggataa tggtcagagg caccagtacc cacgccgacc acagaccagc aacgccgagg
     4441 gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg gtgatagaac
     4501 aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac gaaaagcccc
     4561 ttacttgtag gaggtctgac cactgtgatg atatggttgt agtggatgta aaaacattta
     4621 gcaatgtgtt tacaatataa ttacaacaaa gctcacattg ttgctgtttt tatccgcact
     4681 tcaggtcaaa aagtcctggt catagcacct gcccgtactt ctcgcttttg gcggtatccg
     4741 gtacactgca ttttgtctat tacatttatg ctgaaggata tcctcatgta ccaggatctt
     4801 attcgtaacg aactgaacga agcggcggaa acgctggcta actttttaaa agatgacgcc
     4861 aatattcacg ccattcagcg cgcggcggtc ctgttagcag acagctttaa agccggtggc
     4921 aaagtgcttt cctgcggcaa cggcggttcc cattgcgacg ctatgcactt tgccgaagag
     4981 ttgaccggtc gctaccgtga aaaccgtccg ggctacccgg cgattgctat ttctgacgtt
     5041 agtcatattt cctgcgtcgg taatgatttc ggtttcaatg atattttctc ccgctacgtt
     5101 gaagcggtag gtcgcgaagg cgatgtactg ctggggatct ccacctccgg taactctgca
     5161 aacgtgatca aagcgatcgc agcggcgcgt gagaagggga tgaaagtgat caccctgacc
     5221 ggtaaagacg gcggtaaaat ggctggcacg gcggatatcg aaattcgcgt tccgcacttt
     5281 ggttatgccg accgcattca ggagattcac attaaagtga tccatatcct gatccagtta
     5341 attgaaaaag agatggttaa gtaagtctgg cgtaggccgg ataaggcgtt tacgccgcat
     5401 ccggcacttg tgcgctgatg cctgatgcga tgctgacgcg tcttatcatg cctacaaatc
     5461 tgtacccgaa ccgtaggccg aataattcgt ttacgccgca tccgacctga aaattcttaa
     5521 atcaatcttc gccggggtcc atgcgctccc gctgttgtgg aggttaccca tgtgcgaatt
     5581 gctcgggatg agcgccaacg tccctaccga tatctgcttt agtttcaccg gacttgtaca
     5641 gcgtggtggt ggaaccgggc cacataaaga tggctggggc attacctttt acgaaggtaa
     5701 aggctgtcgc acatttaaag atccacaacc cagctttaat tcccccatcg ccaaacttgt
     5761 ccaggactac ccgataaaat cctgttcggt ggtggctcat attcgccagg ctaatcgggg
     5821 cgaggtggcg ctggaaaata ctcacccatt tacccgcgag ttatgggggc gtaactggac
     5881 ttatgcccat aacggacaac tgacgggcta caaatcactg gaaaccggca acttccgccc
     5941 ggtcggtgaa accgacagcg aaaaagcctt ttgctggctg ctgcataaat taacgcagcg
     6001 ttacccgcgc acgccgggca acatggcggc agtgtttaaa tatatcgcct cactggcgga
     6061 tgaactgcgg gcgaagggcg ttttcaacat gctgctttcg gacgggcgct atgtaatggc
     6121 gtattgctcg actaatttac actggatcac ccgccgcgcg ccgtttggcg tggcaacgtt
     6181 gctggatcag gatgtggaaa tcgacttcag ctcgcagacc acaccgaatg atgtggtcac
     6241 ggtgattgcg acacagccgc tgacgggcaa tgaaacctgg caaaaaatta tgccaggcga
     6301 atggcgctta ttttgcctcg gggagcgtgt agtttgatgc cagttgtggc tgcaccactt
     6361 cgtggcttaa cggcttgctg accacgtaac ggccattggc cacagaaacg gtcggtggct
     6421 tacgggtttg ctcaaagtag tcgtagcccg gcttcagctg ctcccagaag tccttaaagt
     6481 tggaatattt atggcgcttc atattggcgt cggtcatgcg gaatgggtaa atactcacct
     6541 gcacgctcgg ctgaccaaac accagcgcac cagtaacgaa ctggaagatc tcatcaatac
     6601 cctgattggt cattgcgtag cagccgatgg atacacaatc gccgtggatc atcaggtatt
     6661 tcccttcata accatgcgca cggtcataag cattggggaa tccaatatta atcgctttgt
     6721 agtaacggct gtctggtttt aactgattac gctggacgct ataaaatcct tccgggcttt
     6781 tgaaatcgcc ctgacgctgt tttggcccta agccgccgga atatttacag attttataac
     6841 tgtcgagcag ttgatattgc tcgcccattt tgacgtaaag atcgagcgta cgttcttcct
     6901 tgaagatctg gatgtagaca ggggatccca tcaactgctg cttatactct ttgctcactg
     6961 gcgtggtcga gctactgctg cccagcaaac cggcaaacga aacgcacggg atcaacaaca
     7021 tcgcaagaat taatgcgatt ttacgcatac tgcttattcc ttgataaaac ggttacacac
     7081 gccaggacgg caaaatggat cccaaatcgg aatagtctgg atttggaagg ctcacattat
     7141 caccaaaaga gttttacgca agcctgtcgg cgctgggttt acaaatttag cacaggggca
     7201 gttttcaata gtccgaaaag cgcatgtatc atttatttcc taatattatg aaatctcaat
     7261 agtaatatgc aaataagcaa ttaataatcc cgtttactcc tccggtcttt taaaattctc
     7321 accttcattc agccggttta tgtctttcat gtcttcgttc ttgctcggca gatttttgca
     7381 tccaggcgtg ttttcgttat gcgttttgct ccctttactc gccagcgcca ccacaaccgg
     7441 gagcagataa aacagcatcc agaatggttt cctgcgccgc tcaaggcgag tgacagacgc
     7501 tggcaggagc tggcggaaaa caaccacttt ttaagcagcg atcacctgca taacatcacc
     7561 gaagtggcga ttcaccgcct ggagcagcag cttggcaagc cttacgtctg gggcggcacg
     7621 cggcccgatc aaggcttcga ttgtagtggg ttagtttttt atgcctacaa caagatcctt
     7681 gaggcgaaac tcccgcgcac ggccaatgag atgtaccact accgtcgggc aacgattgtg
     7741 gcgaataacg acctgcgccg gggagatttg ctatttttcc atatccacag ccgcgagatt
     7801 gccgaccata tgggggtgta tctgggcgac ggtcagttta ttgagtcact gcgcaccggg
     7861 gaaaccattc gggtaagccg gttagccgaa cctttctggc aggaccattt tttaggcgca
     7921 cgcaggattt tgacggaaga gatgattttg taggccggat aaggcgttta cgccgcatcc
     7981 ggcggttgtg cacggatgcc tgatgcgacg ctgacgcgtc ttatcatgcc tacaaatctg
     8041 tgcgtttttg cgagccgctt tcccgatata acacagcgtc attgtcggcc tttccttttc
     8101 ctcttactgt tgagaaaaag gagtcgctat gtcggaatat cgtcgttatt acatcaaggg
     8161 gggaacatgg tttttcacgg taaatttacg caatcgtcga agccatcttc tgaccaccca
     8221 gttccagacg ctccgtaacg ccattattaa cgttaagcga gacagacctt ttgaaattaa
     8281 cgcctgggtc gttttgccgg agcatatgca ttgcatatgg acattacccg aaagcgatga
     8341 tgatttttcc tctcgctggc gggagattaa aaagcagttc acccatgctt gcggattgaa
     8401 aaatatctgg caaccgcgtt tttgggagca tgccattcgc aatacaaaag attatcgcca
     8461 tcatgttgat tatatttata taaatccaga gaaacatggc tgggtaaagc aggtgagtga
     8521 ttggccattc tcaacgttcc atcgcgatgt cgcgcgaggg ttatatccca tcgattgggc
     8581 gggagacata acggatttaa gtgccgggga gcgtatcatt ttataactgt gcgctggtgc
     8641 cggatgcgct tacgcttatc cggcctacaa atcgtgcttt aacggtaggc cggataaggc
     8701 gttcacgccg catccggcag ttatgcgcag atgcctgatg cgacgctggc gcgtcttatc
     8761 atgcctaaaa acttgtgcct gaacggtagg ccggataagg cgtttacgcc gcatccggca
     8821 attgtgcacc gatgccggat tcttacatca acgcccccat aatcttcaac tccagctcat
     8881 ccggcacttc gttatacgac agcacatgca gccccggcgc aaacaacctt gcataacgcg
     8941 ccagcaaagg gcgtagctgc ggcggcacca gcagcaccgg atctttcccc gccgctttca
     9001 tctgctcttt cacctgtggc atcgtgctct ggaactggtt gagcatattc gggtcaaccg
     9061 gcacgctgtc gagcatcact ttgccggcct gttgcgcctg attcactacg ttggtcagca
     9121 gattttccag ctcattattc agcgtataca ccgtcagctc ctgcttgcga acgaacggat
     9181 gagtaatgct gcgccgcagc gccaggcgca catcggccgc cagcagaata tgatctttag
     9241 tcaccgtgct actggcgacc agcacggtgg cgatggtgac gatatcgcgc agggaaacgc
     9301 cttcggtcag cagcgcccgg tacactttca gcaattggct gtaattgagc gccgcgctta
     9361 aatcctccgc caggcgcggt gccatcgacg acaaacggtt atgcaactgc gtgatgtcat
     9421 catagttaaa caaatcagga atatagctgc gcacaatctt gttcacatgg gtggcaatca
     9481 cactggcgct gtcgatcacc tgatacccca tattcagagc cttcgctttc tgcgcaggct
     9541 ggatccaggt gaccggtatc ccgtacgccg ggtcgttccc cagcacgccg tcaatctcgc
     9601 cgtaggtttc gctggagggc agcgccatca gtttatccgc cggaatatcc gcttcatcag
     9661 ccttaatgcc gttgatgaaa atagcgtact ggctgggctt gaggcggaag ttttcccgaa
     9721 tgcggatctc cggcagcagc acgccgttac cgtcggaaat cacctgccga acgccgcgaa
     9781 tccgctgggt gagcgggttg ccctgcgctt tgtccaccag cgccaccagc ttgtagccga
     9841 ggcttaagct gatgggctcg atcagcggaa tagtttccca actgacctgt tgttcgctgg
     9901 tttcagtgat ggtgcgggtc agcgtttcga ggcttttctc ttccgcctcc gccgcctgcg
     9961 ggcgtttgct catccgccag ccggtaaagc caagcagggc gctgaacagc aaaaacggca
    10021 gatgcggcat ccccggcacc accgccagca cgaacatgat cccggtggcg gtgtagagca
    10081 ccgacgggct tgccagcaac tggtggcgca cgtcatgggc gatatcgccg ttgtcgctga
    10141 cgcgggtgac gataatcgcc gccgcggtgg agagcagcag ggaagggatc tgcgccacca
    10201 ggccgtcgcc gatggtcatc agcacgtatt gctggaaggc ggcgtcggcg ctcaggttgt
    10261 atttgaagat gccgatacag acgccgccgc tcaggttgat cgccagaatc atcatcccgg
    10321 cgatggcgtc tccgcgtaca aacttcgatg ccccgtccat cgcgccgtag aaatcggcct
    10381 cgctggcaac atctttacgc cgggtttgcg cctgcgcttg gttgatcaat ccggcgttaa
    10441 gatcggcgtc aatcgccatc tgtttgccgg gcatcgcgtc cagggtaaag cgggcagaaa
    10501 cctcggaaat acgctcggcc tctttggtga cgacgataaa gttgattgtc aacagcgtca
    10561 gtaaatccga gcgtgaaagc attatcgccg cgctgcacgg gcagtcgatt tttaacggtg
    10621 gtgggctgtc gccgttaaat aaaatcagcc cgtctcatcc gccaaaacct gccaccgtgg
    10681 cggtgccgga agagacggag aaaaaagcgc gtgatgtgaa tgagaaaacg gcgctgctga
    10741 agaagaaatc cgccaccgag cttggcgagt tggcaaccag tatcaacacc attgcccgtg
    10801 atgcgcatat ggaagcgaat ctggagatgg agattgttcc ccagggatta cgcgtgctga
    10861 ttaaagacga ccagaatcgc aatatgttcg aacgcggcag cgcgcagatt atgccgttct
    10921 ttaaaacttt gctggtggag ctcgcgccag tgttcgactc gctctataac aaaattatta
    10981 ttaccgggca taccgatgcg atggcctaca aaaacaatat ctacaacaac tggaaccttt
    11041 cgggcgatcg cgcgctttca gctcgtcggg tgctggaaga ggccggaatg ccggaagata
    11101 aagtgatgca ggtaagcgca atggcggacc agatgctgct ggacgcgaag aatccgcaaa
    11161 gcgcgggcaa tcggcgtatt gagattatgg tactgaccaa aagtgcgtcc gatacgctgt
    11221 atcaatactt tggtcagcat ggggataaag tggtgcagcc gctggtgcaa aagctggata
    11281 agcagcaggt gctttcgcag cgaatgcgct aaatgctgaa tctttacgca tttctcaaaa
    11341 cctgaaatca ctgtatactt taccagtgtt gagaggtgag caatgcgtaa aatcattcat
    11401 gtggatatgg actgcttttt cgcagcggtg gagatgcgcg acaatcccgc tctgcgcgat
    11461 atccctattg ctattggcgg cagccgcgaa cgtcgggggg tgatcagcac cgccaattat
    11521 cccgcgcgta aatttggcgt acgtagcgct atgcctactg ggatggcgct caaattatgc
    11581 ccacatctca ccttgcttcc ggggcgcttt gacgcctaca aagaagcctc aaatcatatc
    11641 cgtgaaatct tctcgcgcta cacctcgcgc attgaaccgt tgtcactgga tgaggcttat
    11701 ctcgatgtca ccgatagcgt ccattgccac ggttctgcga ccctcatcgc ccaggaaatc
    11761 cgccagacga tcttcaacga gctgcaactg acggcatctg cgggcgtggc accagtaaag
    11821 tttctcgcca aaatcgcctc cgacatgaat aaacccaacg gtcagtttgt gattacgccg
    11881 gcagaagttc cggcattttt acaaacctta ccactggaaa aaatccccgg cgtcggcaaa
    11941 gtctcggcgg caaaactgga agcgatgggg ctgcggacct gcggtgatgt acaaaagtgt
    12001 gatctggtga ttctgcttaa acgctttggc aaatttggcc gcattttgtg ggagcgtagt
    12061 caggggattg acgagcgcga cgttaacagc gaacggttac gaaaatccgt cggcgtggaa
    12121 cgcacgatgg cggaagatat tcatcactgg tctgaatgtg aagcgattat cgagcggctg
    12181 tatccggaac ttgaacgccg tctggcaaag gtaaaacctg atttactgat tgctcgccag
    12241 ggggtgaaat taaagttcga cgattttcag cagaccaccc aggagcacgt ctggccgcgg
    12301 ctgaataaag ctgacttaat cgccaccgcg cgtaaaacct gggatgaacg ccgcggcggg
    12361 cgcggtgtgc gtctggtggg gctgcatgtg acgttgcttg acccgcaaat ggaaagacaa
    12421 ctggtgctgg gattatgatg tatactatta tgtatattct ggtgtgcatt attatgaggg
    12481 tatcactgta tgcatcggat tctcgctgaa aaatcggtca atatcactga gttacgtaaa
    12541 aacccagcta aatactttat tgatcaacca gttgcggttc tttctaataa tcgccccgca
    12601 ggatatctct taagtgccag cgcattcgaa gcgttaatgg acatgcttgc tgaacaagag
    12661 gagaaaaagc ccataaaggc gcgctttcgt ccaagtgctg caagattaga ggaaattaca
    12721 cgccgcgcta aacaatatct taatgatatg acggatgatg atttcaatga ctttaaggaa
    12781 taaggatgcg ggtattcaaa acaaaactta ttcgcctgca acttacagca gaggaacttg
    12841 atgcgttaac ggcggatttt atttcctata agcgtgacgg tgttttgcca gatatatttg
    12901 gtcgcgatgc actctacgac gactcgttta cctggccatt aatcaaattt gagcgagttg
    12961 ctcatattca tctggcaaat gtgaataatc catttccgcc acagttgcgc caattcagca
    13021 gaacgaatga cgaagcgcat ttggtatatt gtcagggtgc gtttgatgag caagcatggt
    13081 tgctcattgc cattctgaag cctgaacctc ataaactggc tcgagataac aaccaaatgc
    13141 ataaaattgg aaaaatggca gaagcgtttc gcat
//
LOCUS       DADWTH010000107        12505 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.108, whole genome shotgun sequence.
ACCESSION   DADWTH010000107 DADWTH010000000
VERSION     DADWTH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.108"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(67..2016)
                     /locus_tag="JNY02_003277"
     CDS             complement(67..2016)
                     /locus_tag="JNY02_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBB9434711.1"
                     /translation="MTDSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2101..2437)
                     /locus_tag="JNY02_003278"
                     /pseudo
     CDS             complement(2101..2437)
                     /locus_tag="JNY02_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2437..2793)
                     /locus_tag="JNY02_003279"
     CDS             complement(2437..2793)
                     /locus_tag="JNY02_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBB9434712.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2793..4289)
                     /locus_tag="JNY02_003280"
     CDS             complement(2793..4289)
                     /locus_tag="JNY02_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBB9434713.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4286..4447)
                     /locus_tag="JNY02_003281"
     CDS             complement(4286..4447)
                     /locus_tag="JNY02_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HBB9434714.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4457..5019)
                     /locus_tag="JNY02_003282"
                     /pseudo
     CDS             complement(4457..5019)
                     /locus_tag="JNY02_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5016..5540)
                     /locus_tag="JNY02_003283"
     CDS             complement(5016..5540)
                     /locus_tag="JNY02_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434715.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRYARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5506..5922)
                     /locus_tag="JNY02_003284"
     CDS             complement(5506..5922)
                     /locus_tag="JNY02_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB9434716.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5919..6242)
                     /locus_tag="JNY02_003285"
     CDS             complement(5919..6242)
                     /locus_tag="JNY02_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB9434717.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6287..8017)
                     /locus_tag="JNY02_003286"
     CDS             complement(6287..8017)
                     /locus_tag="JNY02_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB9434718.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGTTSGWVG
                     ETEARQETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7995..9236)
                     /locus_tag="JNY02_003287"
     CDS             complement(7995..9236)
                     /locus_tag="JNY02_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB9434719.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9236..9418)
                     /locus_tag="JNY02_003288"
     CDS             complement(9236..9418)
                     /locus_tag="JNY02_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434720.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9430..11187)
                     /locus_tag="JNY02_003289"
     CDS             complement(9430..11187)
                     /locus_tag="JNY02_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB9434721.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEEWGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11187..11669)
                     /locus_tag="JNY02_003290"
     CDS             complement(11187..11669)
                     /locus_tag="JNY02_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBB9434722.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11817..12167)
                     /locus_tag="JNY02_003291"
     CDS             complement(11817..12167)
                     /locus_tag="JNY02_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB9434723.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12210..>12505)
                     /locus_tag="JNY02_003292"
     CDS             complement(12210..>12505)
                     /locus_tag="JNY02_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBB9434724.1"
                     /translation="QRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQS
                     VREATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT         2824 a         3715 c         2894 g         3072 t
ORIGIN      
        1 aaacaaactc cattgttagc atgttacgcg ataattttgc ctcatctttc accacaaaaa
       61 aagaactcac cttttaaatg gagaataccc aacatcatgg gtgattttca taaagggatc
      121 gcctgttttc ggcaaatcga taacgcgcat tccaggcggc gcgttgtcaa acgtcacctt
      181 aagttcgctg cgggttgccg gtgaagatgc aaggcttaac agctgattcc tttccagtga
      241 tacattcggg gtgtagcgtt tatcgggaga taaataccca tcaccacgtg gcatctgcca
      301 tccggtcatg tcatagacaa aatcatggaa ctgtttgccc cactcgtcga acttatcatt
      361 cagaccaaat ccactgttca gtgcatcagc cacaaaattt ttaacaagcc agggatgttc
      421 tttttcaaac tcctgcgcca tcatgcccag ttcaagcaat ccaccaatca gactgatctt
      481 tccgaggccc tttaatttca gccctgtttt tcctgccgcc gttgtccatt gattctgggc
      541 gactgttgcc gcacgaatac ttgccaccgc cttcacacca atatacaggg cagaaaccgt
      601 tgctattgtt tttactgcct gttcccatcc ccccattgct ctgacaacct gatcaacctc
      661 ctgccagact tttttcacaa ccggaccgac cgtttcccag ttatcaatta tgagatatgc
      721 accaccgacg agaagcgcaa ttaatccctt tgccggcgtc atattcatca cgctacccat
      781 gatttttgtt atgcgtgtca atgtgccaat cgccacgccc atcgtcagta atgccgcacc
      841 agttttcgcg atggttttta cgacctcagg attttctctg acaaacgtgc gcacttcttc
      901 cagaaaaggc ttcatctctt ttatgccttc attgagtgaa ggcaggaagg tttcccccag
      961 cgtggaagaa atcgcattga tctggttctg cagcagcaac aactgatttt ctgtcgtcgc
     1021 ggcacgggcg gcatattctt tctgcattga gccgccatac tgctgcgcat ccgccacacg
     1081 attaaagttg gttcgcaaca aatccagatt cgtgagcagc ggcgcaatgg cccccaggga
     1141 ttccttcccg aacagcgctt tcagcacagc agcctgtttt tctttcggca cttttgccag
     1201 agaatccagt acgtgcagca tggccccgcg ggcgtctttc tgcatatccg ccgccagttt
     1261 tttcgggcta atgcgcaacg agcggagcac ttttttctgc gatttggtgg cagaatccct
     1321 cgctgtcagc gacaacataa aattctttat ccccgtcgca gcaatttctg actccacccc
     1381 cattccggca atggttgccc ccattgcggc aatctctccg gaggccacac ccgcaacact
     1441 gcctaaaggg ccaatacggg tcacaacatc agaaatcttt ttcgcacttg caggaccggt
     1501 attgccaaga tagttaatct tatccgcaag ccctgccacc tctccctgtg tcagtttaaa
     1561 ggcagtgcgc cactgcgcca tcatctgacc ggattcttct gccgtggcgt caaaggccac
     1621 gcccatcttc acggcatcgt cagtaaactg catcagctcg tcacgggcga taccagcctg
     1681 accacccgcc gccacaattt cggcgatacc ttccgccgac atgggcagtt ctgttgacag
     1741 gtcgcgcacc tgctccgtca ttgccttaaa cgcttccggc gtatccagac catccaccac
     1801 tttccggaca tccgccattt ttgattcaag ggcaatggca gatttgaccg ggagcgccag
     1861 cgcccccagt actgcggttc cggcacccgc tgcgcccagt gacaggctgg caaactcctt
     1921 tttaaaacct ttcagctggc gctgcattcc cttcagcggt gccgatacct tatccacggc
     1981 agtgatgatc gccttcagct gaaaactgtc agtcatgctt catctcctcg ttaatgcgga
     2041 cggcctcggc ttccagttct gtaaactgcg aaacagccac ccgtcgcagc tccagtggat
     2101 tcagtttcca gaaccaggca acattgtaga gtcgtttccc ggaggtgctt cccgtctccg
     2161 actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa aatatccagt tttgccatct
     2221 gcgccgccga tgaacgcggg atccccgcca gcaacgggat atatttcagt gccacccggc
     2281 tgtccagttt aatactgcct tcaccggaaa taatgaaagg aaaacccagc gcctcgattt
     2341 cgtcatacgt gggctcgcgc aactccagca catgcagcat ttcgttatgc gctgtcaccg
     2401 gtttttttaa aactatttct gtaacgttca ttgccattac tgataccctc cctcttcgcc
     2461 gtggaattca agatctgccg tgccttcttc ggcattatga ttagcctctc cgtgcagcca
     2521 tgccgccgaa agcacataca ccatgccgtt tgccagctca gcggtaatcg tcatctggtc
     2581 tgaggtggta attttgtcga ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta
     2641 aggtgaccgc caggtctcct tgtaatccac atcccctgcc atgccgacga tgtcatcccg
     2701 gacattggtg ttcatcggca cttcaatgcc acctgtcagc gataactgct gaccatccac
     2761 tttaaaaaaa caggttccgg cgatctttgc cattatgctg actcctctga atactgaaga
     2821 cggaactggt taaccaccgc aaagacacgt aactgattaa cataatccgg cgggaacagc
     2881 gtattcagtc ggttcgggtt atctgcatca cgctcaacta tcagatactg tttaaacaga
     2941 tcgtaatttt ccacaatacc ggcacgctcc atctgacgat atgtcgccag aagttcccct
     3001 ttgataacgg caggagtgac aatcgcctgc cccggaccaa aacgggtacc atcatttgcc
     3061 agcttgtgac gtccgtactt gcttgtgatg accgatttca gtttgcgaag aacatatgca
     3121 ctggtatgca gagtttcact gtccagatag ctgttgtctg ccacgccata cgcattcttt
     3181 ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc cttccacata agccgtcgcc
     3241 acgccgtgag ataaaagggt ctgctgctct gtcatgatga atcgcttacc tttcggtgcc
     3301 ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg caggatcatt ccggataaat
     3361 accgcttcac gggcaaggcg actggcaacc agttcatcga caggcgactg ggtttctttt
     3421 tcgtaaccgg caagcgtgat atgttgctga ttatgcatat ctccggcacc aaccagctct
     3481 gacagcgttc ccagttttgc ggtatagaca tgcccgtata actggcgcgc ataactccag
     3541 cgaccgctgc tgtcattcat ttcggtcatc atcatattga tggaggcggc atcgttgaac
     3601 ggcagaccga taaagtcgaa tgcctcatcg cccatagcag caacagcggc ggtaagatca
     3661 ggcgctccgc taccggcagc accgacttcc gtcaccaccc gaagccctgc tggcagaatc
     3721 tcaccaccac cagaaccata ataattcaga cagaccggta actcattacc atacagcccc
     3781 ttatgacggg cagtcagcgt caccacgcct gcatcagatg acgccgtaaa cggcagggtg
     3841 ataaccccgt ttaccgcttc cttaatcgcg gtggcaaccg cagtggcatc atcgccattc
     3901 accacaggca cctgtacacg ggagcgcccg acatacagac tcagggtgcc gctttcctct
     3961 gcttctccgg caaccgtcac cctgaccgtc gccgccgccc ctctggcttc cggtactgca
     4021 ataacataca gttcaccgaa aggatctgtc tgacggtaga cttccaccat acgcgccagc
     4081 tggctccccg ccccacaaat ctggcgggca taatctgccg atggcatcag caccaggctg
     4141 ttaacctcaa tggctgcatc gttgctggca tgcccgatca gtaatgcagg cgcgctggtc
     4201 accgctgtat ttgccgcaga attgttcatc tcggcgtaga acaacggcac cagcgtattc
     4261 gacggaacag tattaaaact tatcgtcatg attttttagc cttatcctgt ttaacacgta
     4321 ccacatcacc cgccgctata cggcggaacc agtagctgct ttcttccaca tttcgccctt
     4381 cggaaggcaa aaggtctcca cgggcagggt caggaaccga ccgccctttc aggggtttca
     4441 caaacatgag ggactcttaa ttctgaggga aacgcatttc cagatggtgc tcaatgtcac
     4501 catccgggcc agtaccagga tcgatgtaat ccacatcaat actcagcagg gataaatccg
     4561 gcaggcattc acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt
     4621 aaaatcaaac tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata
     4681 aacaatctcg cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct
     4741 ccggacatca tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct
     4801 gagcaccaca atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt
     4861 ttgttccccc ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc
     4921 ggcatcaggg atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca
     4981 tcgctcacgc agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga
     5041 cgtaacgaac gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca
     5101 ataacgtcag ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc
     5161 ttctgccgct tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga
     5221 aacgccggat aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg
     5281 tcaggggaaa ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa
     5341 ccaatggagc gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt
     5401 ccgcgtttaa tcaccagacg tctggcataa cgcatgtaaa cgcgcccgat ttgcacaaac
     5461 gcccgacgca gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt
     5521 aaaaatgctg tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca
     5581 ttccagtaat aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc
     5641 ttggccgtaa taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa
     5701 ataatgcgta acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg
     5761 agccaccttt gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc
     5821 ggtgggttca tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg
     5881 taaaaggtaa gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa
     5941 gggccagcaa gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt
     6001 gagcgatttt cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc
     6061 agatgcatcc cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg
     6121 ttcaggtatg tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg
     6181 tcactgtaca aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc
     6241 atattttcct cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt
     6301 tctggcgggt tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac
     6361 cgccggttcg cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca
     6421 ggatacgggt accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa
     6481 tggcttttgc atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg
     6541 ccagagaaga aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat
     6601 ccttcagcag acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc
     6661 ggtgcgcctt acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag
     6721 ccgcgccgga agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg
     6781 tggactcata agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa
     6841 tttcttcctg ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa
     6901 agaaagcatc atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa
     6961 agggctcaat cagtcccagt tttgaggtgg cggtttcctg acgcgcttcc gtttccccca
     7021 cccatccgga cgtcgtgccg cccagattca ccagtttttt ataatccgag ccacccagag
     7081 tgattacggt ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc
     7141 gatccagttc ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct
     7201 ttcgctcaag ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt
     7261 ctttatgttc cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca
     7321 gctcagcctc aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt
     7381 ccacttctcc gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat
     7441 agcccccggc catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc
     7501 tttcgatttt tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga
     7561 tccgcgcctc gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt
     7621 tcagcagaaa ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag
     7681 aaaggccggt taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat
     7741 catcaataag caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt
     7801 aaacaccgat gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt
     7861 cactccacgc ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt
     7921 cctttacacc aaagacggag ccatgccctt caaactcacc ggagtcactg acagatttca
     7981 gactcagcgg tacatcaaga cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg
     8041 gctttactgc catcggaggg tttcgtggtc atgttcatcg gtgtgagata gacatcccca
     8101 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tgggagagta aatcccccag
     8161 ttaatcccgg tggcgtaggc ttcaaaacgg gatttcatat ccccgcgcag taacgccccg
     8221 gcgttaaatt tggcgtaaaa cttcccctgt ttgctctccc tgaccagccc tgtattgatc
     8281 cgttgttcaa tacgggtcag atacggcaca agggaatagt tgataaatcc gagccccagc
     8341 tcttcaatat tgttgaaagt ggcgcgatcg gtgttctgca ccatgtgcag cggcacgcgg
     8401 aaaagacgac agatttcttc caactgaaac ttgcgggttt ccaggaactg gctgtcctcg
     8461 gcattcagtg ccaccggctt ccagtccagc cccatttcca gaatcatcgg acggtgcgca
     8521 ttacccagcc caagatgacg ctcctcaaaa tccctcttca tgcgctcata agcatccggc
     8581 gtgagctttt gttccgtgcg caacacaccg gatgtcaccg caccattacc aaacaacctg
     8641 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cgcgcgcata tgcaatggga
     8701 ttcagcccga ccagtccgtc cagcgtcagg gtgcgcacat gccagatttc atcctgggtc
     8761 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
     8821 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccacaacttc ccccagcgcc
     8881 ttcaccttgt aagcgtaaaa attcccccgc agacacaggc agacgataac cagctcccag
     8941 aattcctgcg gcgtcatgta gccattgggt tttgctgaaa tcagcttatg cagccgttca
     9001 tccaccgccc gtgttttaag ggtgccgctg attttgtaga gactgcaggg cagcatacca
     9061 acagactcag ccagcaccct gacgcaggaa tagaccgccg tcagccgcat ggcccgctgg
     9121 ctgctgatcc gctttccggt ataggtgtcg tatgacaacc ccagctcttc cgcaagcatc
     9181 ccgggcgttg tgacgggggt gttatttttg cgttgaaaaa gcccctggaa aaacattact
     9241 cacctccgga ggcgacccgg tgaccgcgat cgagataacg cgcaaccagc cacgaccagc
     9301 acagacacag cgccccggca acaacaaaac ctgccggggg ataaatcagc catgtgccat
     9361 acgacagcaa aagcgccccc agcacgccca ccagtggcgt gagaattatc agaaacataa
     9421 tgacctcggt taaagcgagc gaataccaac gctgaccaga tgctcagaca gatccggctc
     9481 cggttcaccg ccattgacca gcatccggct cattgctgta aacatcgcaa cagggccgtc
     9541 gattttggct tccggcgtgg atttgttcgg gaagatattg tcgtttttgt ccggtttcac
     9601 cgtaacgtta gacatcatcc agttcatgac cgggtgatta ctgtgatgga aacgcccggc
     9661 atagaccagt gattccgttt ccttcatggc ctctgacaga ttgcggaccg tctgcggaac
     9721 ttccaccagc ggtatacctt cttcagccag cgccagactg aactgcattg cgctccacgg
     9781 gtcaaatccc agttccctca ggttttcacc gccaatccat tccagtaagt cactttttat
     9841 ctgagcatga tcgataacat caccatccgt cagaatcagc ttatccatct ccgcccactt
     9901 ccggtaaagt tctgcctgct gccgcgagca tcgttccagc cgtccttccg gaagccagaa
     9961 tttaaaatcg gcatgaacat gcccgttatc cgttcgccag agttttgccg ccgcacagat
    10021 atcaatctta tgagcaaggt caacgccgac ccacatggga tacgttttca gctcatgtcg
    10081 tggggcaatg tattcgcact tctcccactt aatcatgtcc atccaggcag actctgctgt
    10141 tacccacaca ttcatgtgtt tggtaaaaaa attcacccgc gcagagacct gttctttcgc
    10201 ttttttcgcc aggcgacgca gatcatccca gcgtttacag atgcccaggc cgggattcgc
    10261 tttctgccag accgtttcat caaacggatc atctccctca tcgagggtgt aaataatcgc
    10321 aaagtaggag tcgtctttta ccgcgccctc cacatcgctg ttatagccac gcaatacctt
    10381 gatggcgtaa tcacgctgct cgtaacaaat cccttccttg ttaaaccctg ccgtggtgat
    10441 accaaataaa agggactgca gacgggcacc ggttgccgtt tccagaacgt cccacacgtc
    10501 acgggtttta tgtgcatgca gctcatcaat aatggcgcag tggatgttca gaccatccag
    10561 gttgtttgca tctgaagaaa gcggttcaaa ttttgatgcg ctctgctcct gataaatcgc
    10621 cagcttgttg aaatcaaaca accgcccgag tgtcgaccgg gcttttctga ccatattttt
    10681 ggcgtcttca aacacgattc tggcctggtc acgcgtggtt gcggctgaat acacctcagc
    10741 cccgccttca ccatctgccc ccgtcatata cagaccgata cctgatgaca gggttgattt
    10801 tgcgttttta cgggcgactt cgttgtacgc tgtccggaaa cggcgcacca tcaccgggcg
    10861 tccgctgcca tcgctgcgca tgacaacttc cccggtctct tcattcacca gcggaatgac
    10921 aaaaccaaaa atattaatga ggataaacac atgccagtcc atcaactcaa tgggctgacc
    10981 tgccagcgcc ccttttacat ggggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg
    11041 gggttcactg aaataaatcc cccactcttc gccgtacttc agatcatcaa gaaaacgctg
    11101 gcaggccaga cggacaaatt caccagcgac aatttctcct gcaacaacac gttcggcgta
    11161 gcggatcccg tcagccactt ttgccatcag tctctcgctt ttaaaagttc tgccagcgga
    11221 tcaacatcat ccggtccggc gatatttact ttcgcccggc ttgccggtga cataccaaat
    11281 tctgcaagca tcgcccggat ccgcttccag gcatccgctt tcatcgcagc agccgggtgt
    11341 gccttaatca gcacatcacc gttctgcgtt tccgtgcggt aggtataacc ctcaacatcg
    11401 agggtttcgc agtgatgccg gtattcggtg taggcttcca ccagcaactc gagtgcacgc
    11461 gcatcaagct gagaaatgat cccttccgca ttcagctctt ccgccattcg cctgaaccag
    11521 tactttccct gcgcccctaa atgctgcgga attttaggga gacctttttc gtccttttta
    11581 gcggtttttt tggcgtcttt aacgggacgc tttgagggat tgcctcgtat caaatgcagg
    11641 cgtggcgggg ttttcggggg tcctgacata atcgatttta cctatcaatc gcttgatcgc
    11701 attcccaaaa aaaagttttc gaacctgcgg cgatgcgagg aagggttggc gggcggtccc
    11761 ggacagccag ggctgcaggg atttgacccg cccctcccta caagtgagaa taattatcac
    11821 ctgattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg
    11881 ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg
    11941 ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg
    12001 gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg
    12061 tgctgcaccc agccttcacc gcgatgctta tcgcagtaac ccgaactgtc tgtggttgta
    12121 cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac
    12181 cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc
    12241 tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc
    12301 cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg
    12361 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
    12421 tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca
    12481 tcgagtgcat taacatcacg ctgtc
//
LOCUS       DADWTH010000108        12372 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.109, whole genome shotgun sequence.
ACCESSION   DADWTH010000108 DADWTH010000000
VERSION     DADWTH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.109"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(73..1309)
                     /locus_tag="JNY02_003293"
                     /pseudo
     CDS             complement(73..1309)
                     /locus_tag="JNY02_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1508..1592)
                     /locus_tag="JNY02_003294"
     tRNA            complement(1508..1592)
                     /locus_tag="JNY02_003294"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Leu,seq:caa)
     gene            1788..2807
                     /gene="ahr"
                     /locus_tag="JNY02_003295"
     CDS             1788..2807
                     /gene="ahr"
                     /locus_tag="JNY02_003295"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBB9434725.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2936..4450
                     /locus_tag="JNY02_003296"
     CDS             2936..4450
                     /locus_tag="JNY02_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBB9434726.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGEKKISQT"
     gene            complement(4558..5640)
                     /gene="lptG"
                     /locus_tag="JNY02_003297"
     CDS             complement(4558..5640)
                     /gene="lptG"
                     /locus_tag="JNY02_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBB9434727.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKDDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5640..6740)
                     /gene="lptF"
                     /locus_tag="JNY02_003298"
     CDS             complement(5640..6740)
                     /gene="lptF"
                     /locus_tag="JNY02_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBB9434728.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7007..8518
                     /gene="pepA"
                     /locus_tag="JNY02_003299"
     CDS             7007..8518
                     /gene="pepA"
                     /locus_tag="JNY02_003299"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBB9434729.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8652..9095
                     /gene="holC"
                     /locus_tag="JNY02_003300"
     CDS             8652..9095
                     /gene="holC"
                     /locus_tag="JNY02_003300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBB9434730.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9095..11950
                     /gene="valS"
                     /locus_tag="JNY02_003301"
     CDS             9095..11950
                     /gene="valS"
                     /locus_tag="JNY02_003301"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBB9434731.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12004..>12372)
                     /locus_tag="JNY02_003302"
     CDS             complement(12004..>12372)
                     /locus_tag="JNY02_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBB9434732.1"
                     /translation="RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRS
                     TLTYHGMLYRMCALVVISEITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQ
                     PDKGFLASISRGVMPSLPFL"
BASE COUNT         3046 a         3238 c         3318 g         2770 t
ORIGIN      
        1 tttctgctca ttattagctc cttctgatgc cattctattt caggaaggag tgtccgttaa
       61 actcaggcta cctcattctg aggtgagcat atcacctctg ttcaggtggc caaattcagt
      121 gtgccacttc aaacatcaac ctacgctccc caagatgttc cgccttatgg atgtaagctg
      181 ctcgcacaga gctacgctcc tggtgactca tctgccgctc taccgcatcc ctcgaccata
      241 atcccgactc aatcaacgaa ctacaagcca tcgtcctgaa gccatgccca caaacctcga
      301 ccttcgtgtc ataccccatt acgcgcaaag ccttgttcac cgtattctca ctcatcggct
      361 tacgtggatc gtgatcgccc acaaaaatca gctttcgatt tccgctcatg cttttgatct
      421 tttccaaaat acttagggcc tgccgcgaca aaggtacaag gtgtggtgtc cgcatcttcg
      481 agccacgctg agaatgcttg actccttcca gttgctcacg ctcacctggg attgtccaca
      541 tagccgtttc aaaatctact tctgaccagc gggcaaaacg cagctcgctc gaacggatga
      601 aaaccaataa agtgagttca accgccagtc gagttaacgg tctgccagaa tagtgatcta
      661 tgcgatgaag taattcaggt atacggttca gttcaagagc tgcacggtgc tgtcttttcg
      721 ccgtagcaac cgcacctgca atctcttgcg cggggttgta gtcgattaag ccgctctgaa
      781 cggcaaagcg cataatcgcg gtagtacgct gctgtaaacg ggcggcaact tcgagccgcc
      841 cggatgactc gactgcttta atgggtacaa gaagatcccg cgtcttcagt tccgcaatgt
      901 tccgcttacc gatagcagta aagagattat cctccagact tttcaaaaca cgagcgctat
      961 gcgatgccga ccacttctga ttgctggcgt gccagtctct ggcgactact tcaaacgtta
     1021 tcgcctcttg ttcctgttct accttaacag ctttcttgtt ttcactggga tcgacaccat
     1081 tagctaaaag cttacgagcc tcatcccggc gtgccctggc atccgccaac gacacttcgg
     1141 ggtatttccc caatgcaagc atcttctctt tgccgccaaa gcgataacgt agccgccagt
     1201 atttggagcc gttagggtga accagcaaaa ccatgccttc accgtcggtc agcttatagg
     1261 cttttgcttc aggcttagcc gaacgaacct tcacatcact cagagccatg atgagtatcc
     1321 tttcaagggt tctgtgtggg tacaaacatt atcgaaccgg gaaatacccg cagttgtacc
     1381 cgcatcagta agttgatgta gattgaatca ggttgactta ggttgagtga aaaagcgagg
     1441 aaagccttgc ggatactgga ttttaggcac aaaaaaagac gtccgttgac gtctattgat
     1501 gttccgatgg tgccgaaggc cggactcgaa ccggcacgta tttctacggt tgattttgaa
     1561 tcaactgcgt ctaccgattt cgccacttcg gcactgaaga ggtatgcgga aaacgttgtg
     1621 gattatacct gtcgcacgcc accatgcaag cgccagcttg cacccacctc gctaagtgct
     1681 gaaattttca gcattacact cagcgatatg taacctttgt cacactccag gccccccgcc
     1741 ctgccatgct ctacacttcc caaacaacac cagagaagga cccaaaaatg tcgatgataa
     1801 aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt ttatgagtac gatcccggtg
     1861 agctgaagcc acaagatgtt gaagtgcagg tggattactg cgggatttgt cattccgatc
     1921 tgtcgatgat cgataacgaa tggggatttt cacaatatcc gctggttgcc gggcatgagg
     1981 tgattgggcg cgtggtggca ctcgggagcg ccgcgcagga taaaggtttg caggtcggtc
     2041 agcgtgtcgg gattggctgg acggcgcgta gctgtggtca ctgcgacgcc tgtattagcg
     2101 gtaatcagat caactgcgag caaggtgcgg tgccgacaat tatgaatcgc ggtggctttg
     2161 ccgagaagtt gcgtgcggac tggcaatggg tgattccact gccagaaaat attgatatcg
     2221 agtccgccgg gccgctgttg tgcggcggta tcacggtctt taaaccactg ttgatgcacc
     2281 atatcactgc taccagccgc gttggggtaa ttggtattgg cgggctgggg catatcgcta
     2341 taaaacttct gcacgcaatg ggatgcgagg tgacggcctt tagttctaat ccggcgaaag
     2401 agcaggaagt actggcgatg ggtgccgata aagtggtgaa tagccgcgat ccgcaggcac
     2461 tgaaagccct gtcggggcag tttgatctca ttatcaatac tgtgaacgtc agcctcgact
     2521 ggcagcctta ttttgaggcg ctgacctatg gcggtaattt ccatacggtc ggtgcggttc
     2581 tcacgccgct gtctgttccg gcctttacgt taattgcggg cgaccgcagc atctctggtt
     2641 ctgctaccgg cacgccttat gagctgcgaa agctgatgcg ctttgccgcc cgcagcaagg
     2701 ttgcgccgac aaccgaactg ttcccgatgt cgaaaattaa cgacgccatt cagcatgtac
     2761 gcgatggtaa agcgcgctac cgagtagtcc tgaaagccga cttctgatcc ctttgccggg
     2821 tgctgtcgcc cggcacttaa atctctttgg aaggcgtctc gcaatctgct gaatttagcg
     2881 gtgaagagtg cacagccgtt gcctatactc caggcaggaa actggaggaa atctcatgag
     2941 cgaacccctg ttaattgccc gcacgccgga cacagaactg tttttactgc cgggaatggc
     3001 taaccgtcac gggctgatta ctggcgcaac ggggacgggt aaaaccgtta cgctgcaaaa
     3061 actggcagag tcattgtcgg aaatcggcgt gccggtgttt atggctgatg tgaaaggcga
     3121 tctgaccggt atcgcgcagg caggaacggc gtcggaaaaa ctgctcacaa ggcttaaaaa
     3181 tatcggcgtc aatgactggc aaccgcatgc caatccggtg gtggtgtggg atatctttgg
     3241 cgagaaaggc cacacggtgc gggcgacggt ttcggatctg gggccgctgt tgctggcgcg
     3301 actgttgaat ctcaacgatg tgcaatctgg cgtgctgaat attatcttcc gcattgcgga
     3361 cgatcaggga ttgttgctgc tcgactttaa agatttgcgg gcgattaccc agtacatcgg
     3421 cgataacgcc aaatccttcc agaatcagta cggaaatatc agtagcgcat cggttggtgc
     3481 catccagcgc ggattactgt cgctggaaca gcaaggcgca gcacacttct ttggtgagcc
     3541 gatgctggat atcaaagact ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat
     3601 cctcagcgcc gagaaacttt atcagatgcc gaaactgtac gccgccagcc tgctgtggat
     3661 gctttcagag ttgtatgaac aattgccgga agcgggcgat ctggagaaac caaaactggt
     3721 gtttttcttc gacgaggcac atctgctgtt taacgatgca ccgcaggtac tgctggataa
     3781 gattgagcag gtgataaggc ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca
     3841 aaacccgtct gatattccgg ataatgtgct cggccagctc ggtaatcgtg ttcagcacgc
     3901 tttgcgggct tttacgccaa aagatcagaa agcagtgaag acagcggcgc aaaccatgcg
     3961 ggccaatccg gcatttgata ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt
     4021 aatctcgttt ctcgatgcaa aaggaagtcc ttctgtggtg gaacgggcga tggtgatcgc
     4081 accttgttcg cgaatggggc cggtgacgga agatgagcgt aatggcctga ttaatcactc
     4141 cccggtgtat ggcaaatatg aagatgaggt ggaccgcgag tccgcctatg agatgctgca
     4201 aaaaggcttt caggccagta ccgagcagca aaataatccc cccgtgaaag ggaaagaggt
     4261 ggcggtggat gacggtattc ttggtggatt gaaggatatt ttgtttggca ctaccggacc
     4321 acgcggcggg aagaaagatg gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt
     4381 gacgaatcag attgtacgcg ggatgttggg gagtttgctg gggggggaga aaaagataag
     4441 tcagacgtga tctgtgcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc
     4501 gaactcgtat ttgactttgt caacaataat aaagggagct ttcgctccct tgttagatta
     4561 cgattttctc attaacagcc acaggctgat taagaagaag ctggcacttg gcaacagtgc
     4621 gccgatgatc ggcgggatgc cgtaaaccaa cgtcagcggg ccgaagatct ggtccagtac
     4681 gtagaagaca aaaccgaagc tgatacctgt gaccacacgc acgcccatcg gtacgctacg
     4741 cagtgggcca aagataaacg acagcgccat cagcatcatc accgccacgg atagcggctg
     4801 gaagattttg ctccacatgt tgagctgata acgtccggca tcctgaccgc tcgacttcag
     4861 atacttcacg tagttgtgca aaccactgat ggagagtgca tccgggtcca gcgccaccac
     4921 gcccagtttg tctggcgtga ggtcggtttt ccaggtgccg ctcaccgtct gcgaaccggt
     4981 aatctgtttc ggattggtca gatcagattc atcaacctgc gacagacgcc agactttatg
     5041 ttccgggtca aacttcgcgg tagcggcata acgtacggat tgcagacgac gattctcgtt
     5101 aaaggcataa atactgatgc cacctaacac ctcgtcatct ttaacccgct caatgtagac
     5161 gaagttgttg ccatctttcg cccataaacc ttgctgggta gagagcaacg agccgccgta
     5221 catcgcctgc gcacggtagt tacgcgccat ctgttcgccc tgcggcgcga cccattcgcc
     5281 aatcgccatc gtcagcaaga ccagtggaat ggcggttttc atcaccgaca gcgctacctg
     5341 catacgggta aaaccagaag cctgcatcac caccagttcg ctgcgctgcg ccagcatccc
     5401 aagaccaagc aacgccccaa gaagagccgc catcgggaag aagatctgca catctttcgg
     5461 cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg taactcccct gcccggcttt
     5521 tttcagctga tcgacaaatt tgataatgcc cgacagcgac accagcatga acagcgtcat
     5581 catgatggtg gtgaaaatag ttttaccgat atagcggtca agtacgccaa aaggttgcat
     5641 cacaccgctc ctttacgcga aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa
     5701 aggttgagaa caatcgccaa agccagataa atcaggttaa cggtccacat ccacagtgtc
     5761 gggtccagct tacctttacc gccgttcgat ttcagggagg tctggatcag gaagaaaagt
     5821 agatacagca gcatggctgg cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg
     5881 ctcaacggta caaccataag tgccatcata aacacggtga ataccaacgt gatacgccag
     5941 ttcagttctg cgcgagcacg atcggtgtca gtgttccaca atgtgcgcat gtccatctgg
     6001 tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa tgatcgcctg ataatcctgg
     6061 aaatccgtaa tgcggaaatc acgtaacagt gcagtgcctt cgaagcgcgt tccctggttg
     6121 agagtgacga cctgggagcc gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc
     6181 acagaaggac gtgcattacc ttttggtcga atttgtgcga ggaacacatc tttgaaatcg
     6241 ctgccgtcaa cgctttcgat gaacagcacc gagctgccat tagtcgcttg ctggaattgc
     6301 ccttgcgcca gcgccgccat gccagggttc gctttcgctt ctgctaacac ttcatcctga
     6361 tgacgcgatg accacggtcc cgcccacatc acgttaaccg ccgcgacgat tgccgtgaat
     6421 accgcaagga tcattgccgc tttcaccaga accgctttgc tcaagccgca ggcatgcatt
     6481 accgtaattt cactttcggt atacagtttg cccagcgtca tcagcagccc gaggaacagg
     6541 cttaatggca ggataagctg cgccatttcc ggcacgccca acccgagaag ggagagcacc
     6601 agattcgccg gaatatcgcc gtcaaccgct gcgccgagga tcctcactaa cttttgacag
     6661 aagaagatca aaagcaagat gaagagtatc gccagctggc ttttgagcgt ctcccgcacc
     6721 agatatctta tgattatcac tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt
     6781 agcttgtttc atggcttaaa cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga
     6841 taattatact caacggattc acctctcaga ttttgttctg acgtgccaat gccgtaataa
     6901 cgttaagatt aacacgaagt catcgcaaca gcggacatga gttacgaaag cttgcaattc
     6961 tatctgtagc caccgccgtt gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt
     7021 aaaaagcggt agcccggaga aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc
     7081 acgtcgcctt tctccgattg cagaacagct cgataaaatc agcgatgggt acatcagcgc
     7141 cctgctacgc cggggcgaac tggaaggaaa accggggcag acattgttgc tgcaccatgt
     7201 tccgaatgta ctttccgagc gaattctcct tattggttgc ggcaaagaac gtgagctgga
     7261 tgagcgtcag tacaagcagg ttattcagaa aaccattaat acgctgaatg atactggctc
     7321 gatggaagcg gtctgctttc tgactgaact gcacgttaaa ggccgtaaca actactggaa
     7381 agtgcgtcag gctgtcgaga cagcaaaaga gacgctctac agtttcgatc agttgaaaac
     7441 gaataagagc gaaccgcgtc gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg
     7501 tgaactgacc agcggtgagc gcgcgatcca gcacggtctg gcgattgccg ccgggataaa
     7561 agcagcgaaa gatctcggta atatgccgcc gaatatctgt aacgccgctt atctcgcctc
     7621 tcaggcgcgc cagctggctg acagctacag caagaatgtc atcacccgcg ttatcggcga
     7681 acagcagatg aaagagctgg gaatgcactc ttatctggcg gtcggtcagg gttcacaaaa
     7741 cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat
     7801 cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt atctccatca agccttcaga
     7861 aggcatggat gagatgaagt acgatatgtg cggcgcggcg gcggtttacg gcgtgatgcg
     7921 tatggtcgcg gagctgcaac tgccgattaa cgttattggc gtgctggcag gctgtgaaaa
     7981 catgcctggc gggcgtgcct atcgtccggg cgatgtgtta accaccatgt ccggtcaaac
     8041 cgttgaagtg ctgaacaccg atgccgaagg ccgcctggta ctgtgcgacg tgttaactta
     8101 cgttgaacgt tttgagccgg aagcagtgat tgacgtggcg acgctgaccg gtgcctgcgt
     8161 gatcgcgctg ggtcatcata ttactggtct gatggcgaac cataatccgc tggcccatga
     8221 actgattgcc gcgtctgaac aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga
     8281 gtatcaggaa cagctggagt ccaattttgc cgatatggcg aacattggcg gtcgtcctgg
     8341 tggggcgatt accgcaggtt gcttcctgtc acgctttacc cgtaagtata actgggcgca
     8401 cctggatatt gcaggaaccg cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc
     8461 ggtagcgttg ctggcacagt tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt
     8521 ttgcgtttta ccctcaccct aaccctctcc ctaagggaga gggaaccgat cgagcgcaat
     8581 agaaattcat tgctcatctt tcagggtgag gaatgcagca atatttaaat ccaccacaag
     8641 aagccccata tatgaaaaac gcaacgttct accttctgga caatgacacc accgtcgatg
     8701 gcttaagcgc cgttgagcaa ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta
     8761 agcgcgtact catcgcctgt gaagatgaaa agcaggctta ccggctggat gaagccctgt
     8821 gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc gggagaagga ccgcgcggcg
     8881 gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag cagcccgcgc gatatattga
     8941 ttagtctgcg aacaagcttt gcagattttg ccaccgcttt cacagaagtg gtagacttcg
     9001 ttccttatga agattctctg aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg
     9061 ctggtttcaa cctgaatacg gcaacctgga aataatggaa aagacataca acccacaaga
     9121 tatcgaacag ccgctttacg agcactggga aaagcagggc tactttaagc ctaatggcga
     9181 tgaaagccag gaaagtttct gcatcatgat cccgccgccg aacgtcaccg gcagtttgca
     9241 tatgggtcac gccttccagc aaaccatcat ggataccatg atccgctatc agcgcatgca
     9301 gggcaaaaac accctgtggc aggtcggtac tgaccacgcc gggatcgcta cccagatggt
     9361 cgttgagcgc aagattgccg cagaagaagg taaaacccgt cacgactacg gccgcgaagc
     9421 tttcatcgac aaaatctggg aatggaaagc ggaatctggc ggcaccatta cccgtcagat
     9481 gcgccgtctc ggcaactccg tcgactggga gcgtgaacgc ttcaccatgg acgaaggcct
     9541 gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa gaagacctga tttaccgtgg
     9601 caagcgcctg gtaaactggg acccgaaact gcgtaccgct atttctgacc tggaagtgga
     9661 aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat ccgctggctg acggcgcgaa
     9721 aaccgcagac ggtaaagatt atctggtggt tgcgaccact cgcccggaaa ccctgctggg
     9781 cgataccggt gtagctgtta acccggaaga tccgcgttac aaagatctga ttggcaaata
     9841 tgtcattctg ccgctggtta accgtcgtat tccgatcgtt ggcgacgaac acgccgacat
     9901 ggaaaaaggc accggctgcg tgaagatcac cccggcgcac gactttaacg actatgaagt
     9961 cggtaaacgt cacgccctgc cgatgatcaa catcctgacc tttgacggtg atatccgtga
    10021 aagcgcccag gtgttcgata ccaaaggtaa cgaatctgac gtttattcca gcgagatccc
    10081 ggcagagttc cagaaactcg agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga
    10141 tgcactcggc ctgctggaag aaattaaacc gcacgacctg accgttcctt acggcgaccg
    10201 tggcggcgtg gttatcgaac cgatgctgac cgaccagtgg tacgtgcgtg ccgatgtact
    10261 ggcgaaaccg gcagttgaag cggttgagaa cggcgacatt cagttcgtac cgaagcagta
    10321 cgaaaacatg tacttctcct ggatgcgcga tattcaggac tggtgtatct ctcgtcagct
    10381 gtggtggggt caccgtatcc cggcatggta tgacgaagcg ggtaatgttt atgttggccg
    10441 caacgaagaa gaagtgcgca aggaaaataa cctcggtgcc aacgttgccc tgcgtcagga
    10501 cgaagacgtt ctcgacacct ggttctcttc tgcactgtgg accttctcta cccttggctg
    10561 gccggaaaat accgacgccc tgcgtcagtt ccacccaacc agcgtgatgg tatctggttt
    10621 cgacatcatc ttcttctgga ttgcccgcat gatcatgatg accatgcact tcatcaaaga
    10681 tgaaaatggc aaaccgcagg tgccgttcca cactgtttac atgaccggtc tgattcgtga
    10741 cgacgaaggt cagaagatgt ccaaatccaa gggtaacgtt atcgacccgc tggatatggt
    10801 tgacggtatt tcgctgccag aactgctgga aaaacgcacc ggcaatatga tgcagccgca
    10861 gctggcggac aaaatccgta agcgcaccga gaaacagttc ccgaacggca ttgagccgca
    10921 cggcaccgat gccctgcgct tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa
    10981 ctgggatatg aagcgtctgg aaggctaccg taacttctgt aacaagctgt ggaacgccag
    11041 ccgctttgtg ctgatgaaca cagaaggtca ggattgcggc ttcaacggcg gcgaaatgac
    11101 gctatcgctg gcggaccgct ggattctggc ggagttcaac cagactatca aagcgtaccg
    11161 cgaagcgctg gacagcttcc gcttcgatat cgccgcaggc attttgtatg agttcacctg
    11221 gaaccagttc tgcgactggt atctggagct gaccaagccg gtaatgaacg gtggaacaga
    11281 agccgaactg cgcggtactc gccatacgct ggtgactgta ctggaaggtc tgctgcgcct
    11341 cgcgcatccg atcattccgt tcatcaccga aaccatctgg cagcgtgtga aagtactttg
    11401 cggtatcact gccgacacca tcatgctgca gccgttcccg cagtatgatg catctcaggt
    11461 tgatgaagcc gcactggccg acaccgagtg gctgaaacag gcgatcgttg ctgtacgtaa
    11521 catccgtgca gaaatgaaca tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg
    11581 cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc ttcctgcaaa cgctggcgcg
    11641 tctggaaagc atcaccgtgc tgcctgccga tgacaaaggt ccggtttccg ttaccaagat
    11701 catcgacggc gcagagctgc tgatcccgat ggctggcctc atcaacaaag aagatgagct
    11761 ggcgcgcctg gcgaaagaag tggcgaagat cgaaggtgaa atcagccgca tcgagaacaa
    11821 gctggcaaat gaaggctttg tcgcccgcgc accggaagcg gttatcgcga aagagcgtga
    11881 gaagctggaa ggttatgcag aagcgaaagc caagctgatt gaacagcagg ctgttatcgc
    11941 cgcactgtaa tcaacacagt gatgaaaacg aaggccggag catgctccgg ccttttttat
    12001 ctcttacaga aatggtaaag aaggcataac tccccgagaa atactggcta agaagccttt
    12061 atctggttgt tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt
    12121 attttttgcc tgccagtcaa tcatccatat tttcagcagt gggtaagcca gaccacccgt
    12181 aatctcggat atcaccacca acgcacacat gcgataaagc ataccgtggt acgttaaagt
    12241 tgagcgaaaa cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa
    12301 cgacaccgtt aaataactgg tgctaacagc aatcccaaaa taataaatta actgcgcgat
    12361 tatcatttta cg
//
LOCUS       DADWTH010000109        12229 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.110, whole genome shotgun sequence.
ACCESSION   DADWTH010000109 DADWTH010000000
VERSION     DADWTH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.110"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..493)
                     /locus_tag="JNY02_003303"
     CDS             complement(<1..493)
                     /locus_tag="JNY02_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9434733.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JNY02_003304"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JNY02_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBB9434734.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="JNY02_003305"
     CDS             complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="JNY02_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBB9434735.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2440..3306
                     /gene="folD"
                     /locus_tag="JNY02_003306"
     CDS             2440..3306
                     /gene="folD"
                     /locus_tag="JNY02_003306"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBB9434736.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKKGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3308..3520
                     /gene="ybcJ"
                     /locus_tag="JNY02_003307"
     CDS             3308..3520
                     /gene="ybcJ"
                     /locus_tag="JNY02_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBB9434737.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3628..4149
                     /locus_tag="JNY02_003308"
     CDS             3628..4149
                     /locus_tag="JNY02_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBB9434738.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="JNY02_003309"
     CDS             complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="JNY02_003309"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBB9434739.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5744..6238
                     /gene="ppiB"
                     /locus_tag="JNY02_003310"
     CDS             5744..6238
                     /gene="ppiB"
                     /locus_tag="JNY02_003310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBB9434740.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6241..6963
                     /gene="lpxH"
                     /locus_tag="JNY02_003311"
     CDS             6241..6963
                     /gene="lpxH"
                     /locus_tag="JNY02_003311"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBB9434741.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7081..7590
                     /gene="purE"
                     /locus_tag="JNY02_003312"
     CDS             7081..7590
                     /gene="purE"
                     /locus_tag="JNY02_003312"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBB9434742.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7587..8654
                     /gene="purK"
                     /locus_tag="JNY02_003313"
     CDS             7587..8654
                     /gene="purK"
                     /locus_tag="JNY02_003313"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBB9434743.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8791..9684)
                     /locus_tag="JNY02_003314"
     CDS             complement(8791..9684)
                     /locus_tag="JNY02_003314"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBB9434744.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9681..10328)
                     /locus_tag="JNY02_003315"
                     /pseudo
     CDS             complement(9681..10328)
                     /locus_tag="JNY02_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10331..11563
                     /gene="allC"
                     /locus_tag="JNY02_003316"
     CDS             10331..11563
                     /gene="allC"
                     /locus_tag="JNY02_003316"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBB9434745.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11574..>12229
                     /gene="allE"
                     /locus_tag="JNY02_003317"
     CDS             11574..>12229
                     /gene="allE"
                     /locus_tag="JNY02_003317"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBB9434746.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     "
BASE COUNT         2966 a         3168 c         3119 g         2976 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga taaacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa gattgctttg
     1261 gcttcttaca acacatcatt cccttttcat ttaataaagc ggcatgatac aaagtgaacg
     1321 tggtttcgat ttacattgcc accataccgc ttcagacaac cggtacatta ctttgttata
     1381 agacgtgata tataattcaa aacaacgtgg ttttgattat tcgtatctca taataaaagt
     1441 cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg gtgcctttat aacgtgcaga
     1501 aaaatgaaga acgttggtcc cggggatcaa gttctggttg gttgagaagc tattcccatc
     1561 aggtttgaga attgcggatg tattatcaag tatttcaata ccgacacccg ttgctgttgt
     1621 tgtatttgta ctggatgcaa tggccaataa attatcattg attgcatcag cctgaccaga
     1681 aaatgcaacg gcagcattag cggcaacaac aggatcgcag tcgttcaact gaatggtgaa
     1741 tggaattaat gcagaggtat taccaacagc attgaaaata tcggtacgat attgaccgag
     1801 tgtgacaacc tggtctgccg agtcagtatt cactgaacag gcagcattca ccagttcgcc
     1861 ttcaaagtga attgtaccgc catctacaac ggcagcataa ctacccgtag cggcgaatag
     1921 tgcggcagcc agcgcagacg aaataaatct taatttcata tatattcctt caatctcatt
     1981 tatcgactcc acatccgtat ataaccgatt actttattta agacactgat agtagtaaaa
     2041 ttccttttta tcctctaaga atgtcttaat tgaaaatatg cactctattc taaaaaatag
     2101 agagccccgt tagatgaata cttccgcgca aaatatattc aacacaaata tagacctgaa
     2161 gcggtaaatt accaggctga aaattctttt tatattgtca ggtatttctt aaattatctt
     2221 aatccttaaa caaggaaata aatcagttcc agatttacaa cgccatcatg tacgaaaaat
     2281 gaagctttca atctcagcga cctcagcgac ggtgcgccta accttcgcaa gaggtcgctt
     2341 cacgcgataa atctgaaacg aaacctgaca gcgcgccccg cttctgacaa aatagacgca
     2401 tccccttcga cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac
     2461 ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggctcgtatt
     2521 gcagccggac tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg
     2581 caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct
     2641 tatgacctcc cggaaaccac cagcgaagcg gagctgctgg agcttatcga tgcgctgaat
     2701 gccgacaaca ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac
     2761 gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttacaac
     2821 gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc
     2881 acgctgcttg agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca
     2941 tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc tcgcaggttg caccaccacc
     3001 gtgactcacc gcttcactaa aaatctgcgt catcacgtag aaaatgccga tctgttgatc
     3061 gttgccgttg gcaagccagg ctttattccc ggtgactgga tcaaaaaagg cgcaattgtg
     3121 attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa
     3181 gacgcggcta aacgcgcctc atacattacg cctgttcccg gcggcgttgg cccgatgacg
     3241 gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacagggt
     3301 gagtaacatg gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct
     3361 gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca
     3421 ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg ccggtcagac
     3481 agtgagtttt gcaggacaca gcgtacaggt tgttgcctga tccctttccc ggcctgtatc
     3541 aacaggccgg ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag
     3601 catcgacatt ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc
     3661 ctttgcattg gtttagggtt gggttcgaaa gtcatccctc cgcgtctgtt atttgccgga
     3721 atcatcctag cgatgctgcc cgatgccgac gtattgtcgt ttaaatttgg cgttgcttac
     3781 ggcaatgttt ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg
     3841 ctcttatgtg tgctgattgg acgtcgatgg ttcagggctg ggctgattcg ctgctggctg
     3901 tttttaaccg tctcgttgct atcgcacagc ttactggatt cggtaaccac tggcggtaag
     3961 ggcgttggct ggctgtggcc gtggtcagat gaacgctttt tcgctccctg gcaggtgatt
     4021 aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt acgggcatca ggtgattatt
     4081 tccgaattga tgtgggtgtg gttgccgggg atgctactga tgggaatgtt gtggtggcgc
     4141 agacgataac cggatgcgaa aactcgcatc cggcaattgc gcaattactt acgacgccag
     4201 gtggtccctt gcgggccatc ttccagcacg atccccatct cgttaagacg gtcacgtgcc
     4261 gcatctgccg ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct
     4321 tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt
     4381 tcttgctcca gcaggcccaa tacagcggaa agtttacgca ggtgagatgc cattgcattc
     4441 gccgctgcca tatcttctgc tttcagacgg ttcacttcac gcgccatatc aaacagcacg
     4501 gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac
     4561 gcttcgccac cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt gtagaggcgc
     4621 tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt agttcagctg gctgcgatag
     4681 tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt agtatttcag cacatcgcgc
     4741 acggtaaaga agttacccag cgatttggac atcttctcgc ggtcaaccat caccattccg
     4801 gagtgcatcc agtagttcac atactgacca tcatgggcac aggtggactg cgcgatttcg
     4861 ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt ggatatcaaa gtggttaccc
     4921 agctgcttgc agttcatggc cgaacattca atgtgccagc caggacgacc cgcgccccac
     4981 ggagacggcc agctcggttc gccctctttc gacatcttcc acagaacgaa gtccatcggg
     5041 ttgcgtttgt catcaaccac gtcaacgcgc gcgccagcct gcagctggtc gagatcctga
     5101 cgcgacagca cgccataagt tggatcggtc gggacgtcga acatcacgtc gccgttgtcc
     5161 gccacataag cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttctgcgata
     5221 tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg
     5281 tgcatttcgg cgatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg
     5341 cgtttgatga ttttgtcgtc gatatcggta atgttgcgca catacttcag tttatagccg
     5401 aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc gtgaccgata
     5461 tgacagagat cgtaaacggt gattccacac acgtacatgc cgacttcccc ggcgtgaata
     5521 ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc
     5581 gtttagacat gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag
     5641 catacattgc gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc
     5701 cgaactgggt tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac
     5761 caatcacggc gatattgtca tcaaaacttt tgacaataaa gcacctgaaa cagttaaaaa
     5821 cttcctggac tactgccgcg aaggttttta caacaacacc attttccacc gtgttatcaa
     5881 cggctttatg attcagggcg gcggttttga accgggcatg aaacaaaaag ccaccaaaga
     5941 accgatcaaa aacgaagcca acaacggcct gaaaaatacc cgtggtacgc tggcaatggc
     6001 acgtactcag gctccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga
     6061 cttcctgaac ttctctggcg aaagcctgca aggttggggc tactgcgtgt ttgctgaagt
     6121 ggttgacggc atggacgtgg tagacaaaat caaaggtgtt gcaaccggtc gtaacggtat
     6181 gcaccaggac gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc
     6241 gtggcgacac tctttattgc agatcttcat ctctgcgtgg aagaaccggc gatcaccgcc
     6301 ggttttctgc gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc
     6361 gatctgtttg aagcatggat tggcgacgac gatcccaacc cactccatcg ccagatggcg
     6421 gcagcgatca aagcggtgtc cgattccggc gttccctgtt atttcattca tggcaaccgt
     6481 gattttctgc tcggcaaacg ctttgcccgt gaaagcggca tgacattatt gccggaagaa
     6541 aaggtgctcg aactttatgg tcgccgggtg ttgattatgc atggcgacac gctgtgcacc
     6601 gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaatgcta
     6661 ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg cgcgaacagc
     6721 aaagaagcca acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgctgtggtc
     6781 agtgcgatgg aaaaacatca ggtgcaatgg ctaatccacg ggcataccca tcgcccggcg
     6841 gtgcatgaac ttatcgccaa ccagcaacct gcttttcgcg tggtactggg tgcctggcat
     6901 acggaaggtt cgatggtgaa agtcacggcg gatgacgttg agctgattca ttttccgttt
     6961 taaaaaaccc acaactttgc tgatttcaca gccacgcaac cgttttcctt gctctctttc
     7021 cgtgctattc tctgtgccct ctaaagccga gagttgtgca ccacaggagt tttaagacgc
     7081 atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg
     7141 gctaccatgc agttcgccgt cgaaatcttc gaaatcctga atgtcccgca ccacgttgaa
     7201 gtggtttctg ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag cgccgaagag
     7261 aacggttatc aggtgattat tgcgggcgca ggcggcgcag cgcacctgcc aggcatgatt
     7321 gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac agagcgccgc actgagcggt
     7381 gtcgatagcc tctactccat cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg
     7441 attggtaaag ctggcgcggc aaacgcggca ttactggcag cacaaattct cgcgactcac
     7501 gataaagagc tacaccagcg tctgaatggc tggcgcaaag cccagaccga cgaagtgctg
     7561 gaaaacccgg acccgcgagg tgcggcatga aacaggtttg cgtcctcggt aacgggcagt
     7621 taggccgtat gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg ccggtcgggc
     7681 tggacgctga accggcggcg gtgccttttc aacaaagcgt gattaccgct gagatcgaac
     7741 gctggccgga aaccgcatta acccgcgagc tggcgcgtca tccggccttt gtgaaccgcg
     7801 atgtgttccc gattattgct gaccgtctga ctcagaaaca gcttttcgat aagctccacc
     7861 tgccgaccgc gccgtggcag ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc
     7921 gtttaggtga actggcgatt gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat
     7981 ggagtttacg cgccaatgaa accgaacagt taccggcaga gtgttacggt gaatgtattg
     8041 tcgagcaggg cattaacttc tctggtgaag tatcgctggt tggcgcgcgc ggctttgatg
     8101 gcagcaccgt gttttatccg ctgacgcata acctgcatca ggacggtatt ttgcgcacca
     8161 gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca agccgaagag atgctgtcgg
     8221 cgattatgca ggagctgggc tatgtgggcg tgatggcgat ggagtgtttt gtcaccccgc
     8281 aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac tggacacaaa
     8341 acggtgccag catcagccag tttgagctgc atctgcgggc gattaccgat ctgccgttac
     8401 cgcaaccagt agtgaataat ccgtcggtga tgatcaatct gattggtagc gatgtgaatt
     8461 atgactggct gaaattgccg ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg
     8521 ggcgtaaagt ggggcatctg aatttgaccg acagcgacac atcgcgtctg actgcgacgc
     8581 tggaagcctt aatcccgctg ctgccgccag agtatgccag cggcgtgatt tgggcgcaga
     8641 gtaagttcag ttaagcaggt gctcatttga ttgccggatg cggcgtaaac gccttatccg
     8701 gcttaccgct ccggtaccca ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     8761 tggtgcaccg attgccggat gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc
     8821 cgccagcgtc tcttcaattc gcgataacgc cccaatccac gcgggtttac tacggcttct
     8881 gacataacca ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg
     8941 cgctaactca tccggtgtgg catggcgaat ggcacgttgc tgcggcgttc cccagttttc
     9001 atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct gctcggcgag
     9061 caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac
     9121 aggcacaccg ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat
     9181 ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccggc gcaaatattt
     9241 accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg
     9301 ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc
     9361 aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca agctctgcgc
     9421 cagcatatag ccaatcatcc cctggctttc cgccaccagc acatccagcg gatacggttc
     9481 tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc cgttgccgtg
     9541 aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag cactggcgat
     9601 attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc
     9661 aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc aagccagaac
     9721 cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca
     9781 gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga ggcgaaatat
     9841 ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgaagattg
     9901 tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata ataccaggcc
     9961 gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg gcctttaccc
    10021 agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac
    10081 tggcgtaatt cagcgggcag cggatcgctt gccgccatcg ccagtggccc gaaaagtccg
    10141 gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga aggcggcgta
    10201 atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct
    10261 tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc
    10321 gtgcggcgaa attacacatt cccgtcaagc tatagaagaa acgctgccct ggctttcctc
    10381 ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg aatggctgga
    10441 aacccagcag caatttaaaa aaagaatggc agcaagtggg ctggaaacac gtttcgatga
    10501 agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag tggttctgag
    10561 cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat tcggcgcgct
    10621 ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcaccgt tacgtacggt
    10681 cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct tctggggcag
    10741 taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg
    10801 aaatagtttt gccgatgcga tgaaggcttg cggatttact ctgcctaacg ccccactaac
    10861 tccgcgtcag gatataaaag cctttgttga actgcatatt gaacagggct gtgtgctgga
    10921 aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc gctatacggt
    10981 gacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc gtcgtgatac
    11041 ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa agatgggcga
    11101 tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa atgtggtgcc
    11161 gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc tgcgcgattt
    11221 cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca ttggtattga
    11281 tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg tcgccaccct
    11341 gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca
    11401 cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc ccagcatcaa
    11461 cgggatcagc cataacccgg cggaacgcac caatattact gaccttgccg aaggggtcaa
    11521 aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca taaatgggat
    11581 atttaaataa cgtcaccggt taccgcgaag atttactggc taaccgtgcg attgttaaac
    11641 acggtaattt cgcactgtta accccggatg gtctggtaaa aaatattatt ccgggctttg
    11701 aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt gttgattatc
    11761 tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa ggcattgaaa
    11821 cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct
    11881 taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt gttaacgccc
    11941 aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta gaaggccatg
    12001 caccgtggct ggtttctggc aatgccagcg aactggaacg cattcactat gaaggtatgg
    12061 atgatgtgat cctgctggat tttctgccca aagagttagg ttttgatatg aacatgcata
    12121 tcctctcttt tgcaccaggt gccagccacg gttatatcga aacacacgtt caggaacatg
    12181 gtgcctatat tctttccggt cagggggttt ataacctcga caataactg
//
LOCUS       DADWTH010000110        12153 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.111, whole genome shotgun sequence.
ACCESSION   DADWTH010000110 DADWTH010000000
VERSION     DADWTH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.111"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            56..1327
                     /locus_tag="JNY02_003318"
                     /pseudo
     CDS             56..1327
                     /locus_tag="JNY02_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            1340..2110
                     /locus_tag="JNY02_003319"
     CDS             1340..2110
                     /locus_tag="JNY02_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBB9434747.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2163..2558
                     /locus_tag="JNY02_003320"
     CDS             2163..2558
                     /locus_tag="JNY02_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBB9434748.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2599..3342
                     /gene="cmoA"
                     /locus_tag="JNY02_003321"
     CDS             2599..3342
                     /gene="cmoA"
                     /locus_tag="JNY02_003321"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBB9434749.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGSAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3339..4310
                     /gene="cmoB"
                     /locus_tag="JNY02_003322"
     CDS             3339..4310
                     /gene="cmoB"
                     /locus_tag="JNY02_003322"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBB9434750.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4475..6904)
                     /gene="torZ"
                     /locus_tag="JNY02_003323"
     CDS             complement(4475..6904)
                     /gene="torZ"
                     /locus_tag="JNY02_003323"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBB9434751.1"
                     /translation="MALTIREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAVMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6929..8029)
                     /locus_tag="JNY02_003324"
     CDS             complement(6929..8029)
                     /locus_tag="JNY02_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBB9434752.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8417..9163)
                     /gene="cutC"
                     /locus_tag="JNY02_003325"
     CDS             complement(8417..9163)
                     /gene="cutC"
                     /locus_tag="JNY02_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBB9434753.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(9177..9743)
                     /locus_tag="JNY02_003326"
     CDS             complement(9177..9743)
                     /locus_tag="JNY02_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB9434754.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9959..11692
                     /gene="argS"
                     /locus_tag="JNY02_003327"
     CDS             9959..11692
                     /gene="argS"
                     /locus_tag="JNY02_003327"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBB9434755.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRSLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11869..>12153
                     /locus_tag="JNY02_003328"
     CDS             11869..>12153
                     /locus_tag="JNY02_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HBB9434756.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNNHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         3031 a         3066 c         2938 g         3118 t
ORIGIN      
        1 tttaatatca tgaaatatgt aagctcgtat cgctcttggg cgtctggagg tatcgatgga
       61 taaagtcaaa tatccaacag gcgtcgaaaa ccacggcggc acattacgca tctggtttaa
      121 ttttaaaggt aaacgtgtca gggaaaatct tggtgtccct gacactgcca agaacaggaa
      181 gatcgccggg gaactgcgga catcggtatg ttttgccatc cgcacaggaa gctttgatta
      241 tgctgcacag ttccctgact cccctaacct tcaggctttt ggggtaagta aaaaagaaat
      301 tacggtgaag gaacttgaag aaaagtggct ggatctgaaa cgaatggaaa tctctgcaaa
      361 tgcattcaat cgctatgaat ccgttgcaag aacgatggtt ccgaaaattg gaggcggcag
      421 actggtgtca tcggtaacca aagaggaatt gctgtatatc aggaaagatt tgctgaccgg
      481 gtatcagaat tcaacgaaaa acaaagcagc agcaaaagga cggagcgtcg ttactgtaaa
      541 ttattacatg acgacaatcg ctggaatgtt tcagtttgct gcagatcacg gttacttaga
      601 agcaaatccc ttccagggaa ttaagcctct taaaagagcc agggcagagc cagatccgtt
      661 aactcgtgac gaatttattc gcctgataga tgcttgccga catcagcaga cgaaaaacct
      721 gtggtcattg gctgtgtaca caggaatgcg tcacggtgaa ctggtctccc tggcctggga
      781 agatatcgat atgaaagcag gaactattac tatcaggcgc aattatacga aactcggtga
      841 gttcactcta cctaaaactg aagcaagtac aaacagggtt gtgcatctta tccagcccgc
      901 tatcagtgtc ctgaaaaatc aggctgaaat gacaagactg ggtaagcagc accacatcaa
      961 ggttcaacta cgtgaatatg gacgttcagt gaatcatgaa tgtactttcg tatttaaccc
     1021 ccaggtggtt agaaaaagca aacaggtagg ttttatctac aaggtagatt ccattggcga
     1081 ctcatgggaa acagccatta agcgtgcggg catcaggcac cggaaagcat accagtcacg
     1141 acacacttat gcgtgctggt cattatctgc cggagcaaac ccaagcttca ttgccagcta
     1201 gatgggccat gcaagtgccc agatggtgtt caatgtatac ggagcatgga tgactgacag
     1261 taatgcagaa cagatcgcaa tgctgaatca gaagctggca gattatgtcc catggtcacc
     1321 aaagtgacac aagagactta ttaaaatcag tgggttagcc ttcaataccc gtcatgttaa
     1381 ctgcgtggag ggcaacacca cgctttacgc cctgccgaaa cccgaggttg tcctgcgctg
     1441 gcgtgaacag accacagatg acttccgctt ctgttttaag tttccggcga ccatttcgca
     1501 tcaggcagca ttacgacatt gcgatgattt agtgactgaa tttttgaccc gcatgtcacc
     1561 gttggctccg cgcattgggc aatactggct gcaactgcct gccacattcg gcccacggga
     1621 gctgcctgcg ctttggcatt ttctcgattc tctccctggc gaatttaatt atggtgtgga
     1681 agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta atcgcggttt
     1741 acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg catcacgtcc
     1801 acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg tacatgctgt
     1861 actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga cgcaaaaccg
     1921 ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca ctacgcctta
     1981 tcttttttta catacgccag atattgccca ggccccggaa ctggtacata ccctgtggga
     2041 agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc
     2101 tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta aagggagttt
     2161 gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg
     2221 atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt tttagcgaac
     2281 tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgc
     2341 tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg
     2401 ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg tttcgctggc
     2461 gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt
     2521 ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt
     2581 ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga
     2641 ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt
     2701 tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt
     2761 caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac gctctcggtg
     2821 cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc cccggcgatg
     2881 attgaacgct gccgtcgtca tattaacgcc tataaagccc ctacgccagt agacgtcatt
     2941 gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc
     3001 ctgcaattcc tggaaccttc cgagcgccag gcattactgg ataaaattta tcaaggactg
     3061 aacccgggca gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt
     3121 gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag
     3181 atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt ggaaacccat
     3241 aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca gtgctttaac
     3301 tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt
     3361 attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc gcgcagattg
     3421 ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac acggtggaat
     3481 ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc gccgaaagcg
     3541 aagagccact gagcaccggg caaattaaac gcattgaaac gcttatgcgg aacctgatgc
     3601 catggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg
     3661 actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc accattctgg
     3721 atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg
     3781 cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg cgtaaactac
     3841 tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacagctt ccggcactga
     3901 aagcctttga taccgtcttt tcgatgggcg tgctttacca tcgccgttcg ccgctggagc
     3961 atctctggca gttgaaagat caactggtga atgagggtga actggtgctg gaaacgctgg
     4021 ttattgatgg cgatgagaac acggtgctgg taccaggcga tcgttacgcg caaatgcgta
     4081 atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg
     4141 tagatatccg cattgtggat gtgtgcgtta ccactactga agagcagcga cgcactgaat
     4201 ggatggttac cgaatctctg tccgatttcc tcgacccaca tgatccgagt aagacagttg
     4261 aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa aggtctggta
     4321 ttactgccgg atgcggcgtg aacgccttat ccggcctaca aacccttgct aattcaatct
     4381 attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagtttta cgtttgcata
     4441 acctcagcgc ccgtttccgg gcgctattca cttcttacgc ctgaacagca ggcttatcaa
     4501 atgccgttaa cttcggtgcg ttacccgtat atttttcgat atacaccagc gcagagttac
     4561 cggcacaggc atttgccagc tgcgagctgg ggatatccgc cgttaacacg ttcgcactgc
     4621 cgtttttaca caagccgttt tccagatctg gccatgcacc ttcatgcacg cataccacgc
     4681 cttttttgat cccgtcagtc accaccgcgc ctgtcaaaat ctgaccacgt ttgttccaga
     4741 cacgcaccag atcgccattc gcaataccaa atcgagcagc atcttcggtg tgaatagtga
     4801 tcggttcacg atcggcaacc gcatattttt tacgcagttc cgcatagtta agctgactgt
     4861 gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg gcggtaccct
     4921 tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt
     4981 ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca tttttcaccg
     5041 gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg cggcgcattt
     5101 cgatcagttt attttgctgc cagaaggcat taaacattgg catagtgacg cgttgcgcac
     5161 gggccccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca tctttacctt
     5221 cggtatagat ctcttttccg ccaggtttga gtaattccgc aagatcggca aacacgtcaa
     5281 aatcgttacg cgcttcaaat tgcggagcaa cagcctgctt catcggcaca atatgctggt
     5341 tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg atcggtaata
     5401 cgatatctgc gtgtttggct gctgcggtcc agtagcattc agaaacgacg atcatctccg
     5461 gtttctgcca ggctttgatc agacgattag tatcctggtg atgggtaaag ttaccgccac
     5521 ccgcccacca gatcattttg atattcggat acgtttgttc tttaccgtta tgctgatatt
     5581 tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc gtcattccgc
     5641 catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc ggcagcacgc
     5701 cgccaacacg catcgggttg ccaccattgg agtaatgata agagaaacca aaaccgccgc
     5761 ccggtgtacc aatctgccct aacatcaccg ccagcgtcac cagcatccag tgtttctgtt
     5821 caccatattg ctggcgctga attccccagc ctgccatcag aattgtgcga ttcgccgcca
     5881 tcagttcagc gagtttgact atctgcgctt ccggtacgcc agtaatttcc gccgcccaga
     5941 ccgcgctctt cggcgtatta tcgctcttac ctgtcagata ctcttcaaac tgcggatacc
     6001 cggtagtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg
     6061 caatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag gtggcattat
     6121 cgccaaagaa ttcgatggtt tcggagcgga taggatcaat ggcaatcact ggtttgccag
     6181 attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt
     6241 ttaaggtatt cagcgggttc attccccaca gcaccacaac ctggctgttt tccagaatca
     6301 gcggccagga ggcctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca
     6361 cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc gccaggttca
     6421 tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc cagccgtaag
     6481 atccggcaaa aatggccgat ggtccgttag ctttacgaat acgctcatgt tgctcatgaa
     6541 tcagttttaa tgcctgttcc cagctcacct gtacataggt atcttcgcca cgacctttcg
     6601 ccggttgcag tggattgtcg agatagcttt ttctcaccat cggatgctga atacgcgccg
     6661 tggtgtgtac ctgatccgcc gccgtagact gtaaggagtt cggtatggtt ttcgccagcg
     6721 cgcctgtcga agaaacaatc ttgccgtcct tcacttctac gctcatcgct ccccaacgtc
     6781 ccgcggtgag gattttaccg cccttctctt ctgcccatgc ggggagcggt gctgccgatg
     6841 tcaccaccag cgctccagcg gcaataccgc tgtgtttaat aaattcacgt attgttaatg
     6901 ccataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct ttggcgttgt
     6961 actggaaata ccgcgttaaa atgtccagtt cgttttcgct catgctggtt cgtgccccca
     7021 ttcctttggc aatggacggc cacgcattga cggtgtaatg gtcggcggca atagaggcat
     7081 gacaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc ggtttacggt
     7141 ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat tgattgccgt
     7201 attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct tcacttaatg
     7261 tggctagcgc cagccgttga cctgccgcca ggtagagcgt gttttcactg ccctgcattt
     7321 gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg gtgagatcgg
     7381 taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga taaatatgtg
     7441 cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg ttgtcatcca
     7501 tttttatttc tggcggaaaa tgggcaatgc ctttatgaca atcgatacag gtttcgccgc
     7561 ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca atatccatgg
     7621 catcaaaaga atggcaacta cggcacgttg cagagtcagt tgcttttaat tctttccata
     7681 ctgtttcggc catttcctgg cgatgagttt cgaacttatc gtcactgtct attttgccgc
     7741 taacaaattc atgataaata tctttagatg cttttaattt agcaaataaa taatccatcc
     7801 ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg ttcgaaaagt
     7861 ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg
     7921 ctgtatccga cgttttatgt aaggcttttt gcgccagcaa taacccgccg ccaccaacca
     7981 caaccgctat cagcaaaaac aataacccaa tgcgtttttt ccctcgcata gattcctcta
     8041 aataaattcc agaacaccga aatcattgta taattaaata cacaacgaat taaccgcttg
     8101 cataactcgg cacaacactg cctgaaataa tcgataaaga atatgattta ttacaatgta
     8161 atcattaatt gctaaggaat aacccagttg ctatttagaa tatatattta tcagttttag
     8221 caatttaaat cccataatca atgtgaatat aaaaaatatt tttagcacaa cgctatgcgc
     8281 actgtcaccc gctcaacaga tcacggttat cgttcgtttt ttatactgct cagggataaa
     8341 aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat
     8401 gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa
     8461 ccgccgcccc gtctacgata taacgcgaat actcgtccgc gtgttcatct gatgacatgg
     8521 acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga
     8581 cttccagcac tccggcatcg aggaagtggt gcaggttttc tgcacggact cctgctccag
     8641 ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca
     8701 gtgcgtctga tttttgccct gatgtcagta cccgggcaat gcccaattcc gcaagattat
     8761 tgagtgtata taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg
     8821 gaccggcagc agccattatt ttttccattc gtggcatatc gacattccca tcaacatcga
     8881 gaacgcccgt caccagtcca ggaaaaccta attcgcggac cgtgcgcaca tcctcaagaa
     8941 tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga attatcggat
     9001 gcacagggat cgtcacccgc tggcgcacgg atttcagtcc ccccaacgac ggcgttaagc
     9061 ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc tgcgccgtta
     9121 gtgcacattc catgctgtaa cagcaaattt ccagtaatgc catttttgct ccttaattac
     9181 gctgactgct cgctggcaac gatctcttca atggaccacg gatgaaactt aatagttgtt
     9241 ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct catgctcacc ttttggtgaa
     9301 ctggttttaa ctgagatccc cggtaggctg agtccttcat cagaaagcaa tgccagcgca
     9361 cgggcattca gcgtttctgg atcacccggc agaacgattt caatatgttc ccaaccttcg
     9421 tgtgggtaac gtttttcccc aggccacggt agctccacat tggaaaactg ccagtgcgca
     9481 acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat cattttttct
     9541 gacaaaagct cgccacactg ttcaaaaccg cgacgccagc gctcagcggt gacgttttga
     9601 tggcaacgca aagaaatgtg atcggcagtc agcggagtga tatccaaccc cagacggcgg
     9661 gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg
     9721 tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc gttaatttac
     9781 cctgttgccc tgtgccaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa
     9841 cggcaatacg ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccaccc
     9901 taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag gtattccggt
     9961 gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc
    10021 tgcggattgc gaaccgcaga ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc
    10081 taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca
    10141 ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg
    10201 ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc
    10261 gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc
    10321 gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta ttggtgacgc
    10381 agcagtgcgt agtctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga
    10441 ctggggcact cagttcggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc
    10501 cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga
    10561 cgaagatgaa gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga
    10621 cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccaaat
    10681 cacttacgat cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta
    10741 caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag
    10801 cgaaggggcg accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg
    10861 cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc
    10921 gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg tattacatcg actcccgtca
    10981 gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc
    11041 cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa
    11101 aacccgcgcg ggtggtacag tgaaactggc agacctgctg gatgaagccc tggagcgtgc
    11161 acgtcgtctg gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc
    11221 taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga
    11281 ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca
    11341 gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaagca gaaattaacg aagagcaact
    11401 ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct
    11461 gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg taatgtgtgc
    11521 ttacctgtac gatctggccg gtctgttctc tggcttctac gagcactgcc cgatcctcag
    11581 cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac
    11641 gctgaagctg ggtctggata cgctgggtat tgagactgta gagcgtatgt aatcgatttt
    11701 tcgtgagagt gaagcctgat cagggttagc cgatcaggct tttttattgc catctaaatg
    11761 tattctgaaa acggacatgc cattgttttc tcactgttga gtaagaggcc agaagcgtaa
    11821 tatccaggcc caggaaaacg ataactgttc aatttaagga ataccgaagt gtttaaattt
    11881 cttgtattaa cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagta
    11941 aacaaccatg acacttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact
    12001 gacattaacg tgatcaaatc atgcctgcga caagaataca atctcgtcga tacgcaatta
    12061 aataaagcct atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgcaaaa
    12121 aaacttgata ctgaacaact taacttgctt aaa
//
LOCUS       DADWTH010000111        12153 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.112, whole genome shotgun sequence.
ACCESSION   DADWTH010000111 DADWTH010000000
VERSION     DADWTH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.112"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            128..997
                     /locus_tag="JNY02_003329"
     CDS             128..997
                     /locus_tag="JNY02_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBB9434757.1"
                     /translation="MAISIKGVNTGVIRKGNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="JNY02_003330"
     CDS             complement(1200..1553)
                     /locus_tag="JNY02_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBB9434758.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNY02_003331"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNY02_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBB9434759.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="JNY02_003332"
     CDS             complement(3381..3674)
                     /locus_tag="JNY02_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBB9434760.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="JNY02_003333"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="JNY02_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBB9434761.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNY02_003334"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNY02_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBB9434762.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="JNY02_003335"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="JNY02_003335"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBB9434763.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /gene="dcuA"
                     /locus_tag="JNY02_003336"
     CDS             7589..8890
                     /gene="dcuA"
                     /locus_tag="JNY02_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBB9434764.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="JNY02_003337"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="JNY02_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBB9434765.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /gene="dsbD"
                     /locus_tag="JNY02_003338"
     CDS             9320..11017
                     /gene="dsbD"
                     /locus_tag="JNY02_003338"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBB9434766.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="JNY02_003339"
     CDS             11054..11629
                     /locus_tag="JNY02_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB9434767.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="JNY02_003340"
     tRNA            11736..11811
                     /locus_tag="JNY02_003340"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2923 a         3133 c         2990 g         3107 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agggtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgattg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa caccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg gatgggttgc gaatcgcgtt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgttagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atataatatc
     7561 aacttgttaa agaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aactatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10681 aacgatagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggacct ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aac
//
LOCUS       DADWTH010000112        12054 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.113, whole genome shotgun sequence.
ACCESSION   DADWTH010000112 DADWTH010000000
VERSION     DADWTH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.113"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..537)
                     /locus_tag="JNY02_003341"
     CDS             complement(<1..537)
                     /locus_tag="JNY02_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9434768.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASM"
     gene            545..1055
                     /gene="tnpA"
                     /locus_tag="JNY02_003342"
                     /pseudo
     CDS             545..1055
                     /gene="tnpA"
                     /locus_tag="JNY02_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1073..1741)
                     /locus_tag="JNY02_003343"
     CDS             complement(1073..1741)
                     /locus_tag="JNY02_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBB9434769.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2044..2637)
                     /gene="tehB"
                     /locus_tag="JNY02_003344"
     CDS             complement(2044..2637)
                     /gene="tehB"
                     /locus_tag="JNY02_003344"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBB9434770.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2634..3626)
                     /gene="tehA"
                     /locus_tag="JNY02_003345"
     CDS             complement(2634..3626)
                     /gene="tehA"
                     /locus_tag="JNY02_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBB9434771.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3750..4730
                     /locus_tag="JNY02_003346"
     CDS             3750..4730
                     /locus_tag="JNY02_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBB9434772.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4725..5261)
                     /gene="rimL"
                     /locus_tag="JNY02_003347"
     CDS             complement(4725..5261)
                     /gene="rimL"
                     /locus_tag="JNY02_003347"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBB9434773.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5324..5548)
                     /locus_tag="JNY02_003348"
     CDS             complement(5324..5548)
                     /locus_tag="JNY02_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBB9434774.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5688..7343)
                     /gene="opgD"
                     /locus_tag="JNY02_003349"
                     /gene_synonym="mdoD"
     CDS             complement(5688..7343)
                     /gene="opgD"
                     /locus_tag="JNY02_003349"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBB9434775.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7567..8910)
                     /locus_tag="JNY02_003350"
                     /pseudo
     CDS             complement(7567..8910)
                     /locus_tag="JNY02_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            9127..10050
                     /locus_tag="JNY02_003351"
     CDS             9127..10050
                     /locus_tag="JNY02_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9434776.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(10088..11728)
                     /gene="trg"
                     /locus_tag="JNY02_003352"
     CDS             complement(10088..11728)
                     /gene="trg"
                     /locus_tag="JNY02_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBB9434777.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
BASE COUNT         3094 a         3022 c         2818 g         3120 t
ORIGIN      
        1 cattgatgct gaagggtgag ccggagtgga tacttcactt tctgtctgaa tactgatgta
       61 ccacgtaccg caggactggc tgacagtgac atttttcaca acacccgtga cctgacggct
      121 attccggtag cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc ggaatgcatc attctgtccc cgctttttga atcggggaaa
      241 agcagcccgc ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggagaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac
      361 cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
      421 ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
      481 acactcctgt tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg
      541 tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt
      601 tttcctgatg catgttcacc tggtctttgt caccagatac cgacgccaga tttttgacca
      661 tgacgcgaca gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga
      721 actggttgaa atggatggcg aaccagatca cgtccatttg ttaattaatt aacctcccaa
      781 actggcgata tccagtctgg taaacaaaca gcctcaaagg cgtatcgggt aggttactgc
      841 gacgagatcg accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg
      901 gctattttgc cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac
      961 aacagcaaac acctggtcag gtggaaaacc gcgccttata tccccgccct gaaggatggg
     1021 gttttacggc gcaccggata aaaagcgcca tctaacgatg gcgcgtctgg aactactttt
     1081 tgttaacgtc aaacatggtg gcatcggtcg ccatgtcatc aataacttgt ttaatatttt
     1141 cgaaagccat tggcgtactt tcgttattca ggtctttacc ttcgccctga cgaacgactt
     1201 tgataaccgg tttattagtc gctgcatcaa tcagctcacc ttcaaaatag aggcgagtat
     1261 ccatggtacg gtggcctgta gccatttgcg tccccgcaac cactaatgca acaggaacca
     1321 cttcatagaa ttgcagccct tctttgctgg tatctacacc ggtaatggcc ccacggaaaa
     1381 tcagactacg cggcccagcg gtggtaacca ctggtttacg ctgcgctatc gcttctttca
     1441 tttcggtgtt ggtatagttc aaaattttat ccagaacttt ctgccctacc tgggtagaag
     1501 gttttggtac cggataataa gtgattgggt tccagacgat gctgtcatat ttgctttgat
     1561 caaaactcgg gtctacccaa cgtaaaacag gtttacctgt tgctgaggtt gtttctttta
     1621 aatcagagta attgtttaaa aaaccagaat atttatctgg ctgggtgatt ttagaggcac
     1681 aaccagacag agccaacaag ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca
     1741 tgaatagttt cctgagatga ttatattact ttctaagtta tagcaaaaag cgccgcttcg
     1801 tgttgtggca aaaaaagtgt tggtgaaaat aaatttttca gccccttttc agacgacata
     1861 gtcaatgccg aaaaaggggc taagtcaatc atcagtagtt aaatttcagt aagttaagct
     1921 gctaaaaaac ttcgactact gtgattaatt tcacatccct tcgataaaaa atatgcttac
     1981 cgaaaagaag aacagtaaat catctcagcg tggggaattt tcacgcgttt tgtgcttacc
     2041 gggtcatttt ttacgtgcca gcatcgtggc gaaacgcagt ttaatacgat taccgttggc
     2101 gtcggtgcgg tgcagctcgc cgacgtcttc attgtatttc accctctccc agccttcgta
     2161 atatcgacgt aattctccct ctttgaaggc aaacgtgaag ccgacggtac atggataatc
     2221 agcggtatcc atcgccgcca caatcaagtt gtaaccacca ggtttagtgc aacgttgcat
     2281 attggcaatc aaccctggga tggttttagc ctcaaggaac atcagcacca cagtcgaaag
     2341 aataaaatcg tactgtccat caaatgtgag gttattcaga tcaacgaccc gggtgtgtaa
     2401 attatccaga ttttcaatgg atttaatgcg ctcgacgttg gcgatactca tggcattttt
     2461 atcccatgcg tcaacatcat aaccattggc tgccaggtaa agactgttac gaccattgcc
     2521 acagcccaga tccagcgttt tacccggttt aaccactttc accgcttcca gtacttcaga
     2581 gtgtgtgcgg gttaattcat atttatcagt aaaatagttt tcgtcacgaa tgatcattct
     2641 ttgtcctctg ctttcattaa aacggcgcgc tcggttctga ctaacaattt tccctgcatc
     2701 agaagcgcaa aagtacggat gagcagtatt gcaataataa aattggtaaa gataaacagc
     2761 ggcaccgcca gcgtatggaa aaatccatta tcgctgccac tccccagatg caaaccggtg
     2821 gttgccagtg cagatacgcc gaacgagaaa ctccagaatg aagcattaaa tggctgggag
     2881 agataccatg gcatcagacg tagcataaac agcagttgca gcagtccata accgaaaagc
     2941 attttcgcca gcgtgtcacc ctcgccgccg ttgacgctca gccaggcact acaggccacc
     3001 agcgcaggag cgagctgaat gccgagtgat gtccgcagtg ccgtgggtaa ttctcccgaa
     3061 ctgcgcagac gctgcaagat caccggttca aggcttagcc atgagaaaac gcctgcgcct
     3121 aaaaacacca gaccggcgtc ggtgtagccc aacgcaccac aggccattgc gctgataaag
     3181 ttgttggcaa ctgtcggcag atacagtcca ggcgtggtag cttcctcagg gtgagatccg
     3241 cgccataatc ccgcagtttg ccaggcggca taagccaact gaaccacgac accaaaactg
     3301 aacatgcaca ccgccagtgg gcgaaaccac ggaacaaaac caatcgccac cagcatcgtc
     3361 gttgccggaa acaaactcac aaaactgctc agcactggat ggcgaacttc cgccagcacg
     3421 ctatgcggaa agcgtatgag tcgggtaata aatgcgctag tcaataatcc ccagatgatc
     3481 atcgccagaa tcaccagccc atcccctaac cagtggctga cctgccaaac ctggctggca
     3541 tagcgccagg caaatcccat ccctatcgtc cccaacacaa taccaaagta gcctgccggc
     3601 aaattgagca ctttatcgct ctgcatcttc ttactcattt tgtttaattt ataaagtata
     3661 tttgaaatgc attttatgga attttctcga gcatagccag agccgcagaa tttgctacgg
     3721 ttatagtaag tcacctgtgg gaaaacgcta tgcgcaaata tcgcctaagt gaagaacagc
     3781 gagcctttag ttatcaggaa gatggcacta aaaaaagtgt gttattacgg cagattatcg
     3841 ccattagcga ttttaacgat gtgatagcag gaaccgctgg cggctggatc gatcgcgaaa
     3901 cggtgttagc acaggaagga aattgctgga tttacgacca gaatgctatt gcgttcggcg
     3961 gaacggtgat ttccggtaat acgcgtatca ccggcaccag cgtgctgtgg ggagaggttt
     4021 acgcaacgga taacgtctgg atcgataata gtgaaatcag ccagggtgca tatataagtg
     4081 acagtgtcac cattcatgac tcattagttt acgggcaatg tagaatattt ggtcacgcct
     4141 taattgatca acattctatg atcgtcgccg cacaaggctt aacgcccgac catcaactcc
     4201 tgctccaaat ttatgatcgc gcaagggtta gcgcctcgcg tattgtccac caggcacaaa
     4261 tttatggcga tgccgtcgtc cggtatgctt ttattgaaca tcgcgccgaa gtttttgatt
     4321 ttgccagtgt tgaaggtaat gaagagaaca acgtctggct atgcgattgc gctaaagtct
     4381 acggtcacgc gcaggtgaaa gctggcatag aagaagatgc cattccgacg attcattaca
     4441 gttcgcaggt ggcggaatat gccattgtgg aaggtaattg tgtgttaaaa catcatgtct
     4501 tgattggcgg aaatgccgtg gtacgtggtg gaccgattct gctcgacgag cacgttgtaa
     4561 tacaaggtga gagccgtatt acaggtgcgg tgataattga aaaccatgtg gagttaaccg
     4621 accacgccgt cgttgaagcc tttgatggcg acacggttca tgtccgtggt ccaaaagtca
     4681 ttaacggcga ggagcgcatt acgcgaacgc cgctggcagg gttattatga atcgataata
     4741 cgcgcgtata agttcacatc atcataggca tcattcagga actcagcctg tttcaggcaa
     4801 ccttcaagga taaaaccatt gcgcaaagcg acctggttgc tttgcggatt gtccacccga
     4861 catttgatca cgaagcgtct aagttcacca gactgagcgt aatgatgaat caatgcctgc
     4921 agcgcctgag aaatgatccc ctgcccctga tgagattcgt ccagccagta gccgatttca
     4981 gcagttttat tcagtggttc aatacgatta aacgagataa cgccgataag ttcatcttct
     5041 ttgaaaatca tgaacatttt ggcatagccg cgttgatgca acatcacatt accctgcacc
     5101 gtttttcgcg tgtcctcttc actctgaacg aactgcggcc agtttagcga ctgctgtaac
     5161 caggttttat ttttacagat taactgataa agaggtgtga cgtgactttc cgcaacagca
     5221 tgtaattcaa gtgattcgct tacttttatc gtttcagtca tcttatctcc agtcaatcca
     5281 tgctgttgtt aaaaaagccg cctggctggc ggcttaggaa agtttacacc tctttgacgc
     5341 tctcctgctg caggattttg agcatctcat cttttaactg tagcttttgt tttttcatgc
     5401 ggaccacttc cgcattgtac cctcgaccgt cggaaccttc ctttctggca atttcatgat
     5461 caagtttatt gtgtttatcg aacaaggaca taaagcgagg attttcgttt ttcagacggg
     5521 atattaaatc tcggtattct ggaaacatac gcactccctg ttagtggctg acacggtgta
     5581 tctgaaatac acaccatcag attactcatt taacttaggg aaaaatgcga gcgggatcgt
     5641 gtttaaccag acggcacctc ccgaagaagg tgccgaagaa aaaacgatta actcatcacg
     5701 cggtcgtcaa catactgacg tttatccggc gctggcggga aatactgata cagccatgtt
     5761 tcactgatag cgtccccctg acaacgtaga tacatccgca tatcgaccgg atcagtggag
     5821 tccgaagtcg gataccagtc aaactgaata cgataaccat cgatgggttc aatatagaga
     5881 atttcgattt gcttcgcttc cccactggaa agcgtaatca ccggctcaat gccttttggc
     5941 gcggcagctt tcagatcacc accaacgaaa tcgacggcaa aacgacgcgc ccatttttcg
     6001 ggatagtgtt cacctggcgc ccaaccttcc gggaaaccgc ccatgccggt acgcgtcgcc
     6061 ataacgcgcg ctaatgggca atgaacaggc ggttgcgcac tccagtacag acgatactgg
     6121 aatgcaaact catcacctgc ttttacagct ttttctggct gccagaagca gacaatgtta
     6181 tccagcgttt cgcccgttgt tgggatttcc atcaggccga tggtgccctt accccacttg
     6241 ttacgcggtt ccacccacag gcttggacgt ttgttatacc agcccataat gtcctgataa
     6301 tgggagaagt cacgatccag ttgcaataaa ccaaaccctt tcgggttgtt gtcggtgtaa
     6361 gcattgaact gcaatttttg cggattattc agcggacggc aaatccactc gccgttgccc
     6421 cgccacatgg acagacgatc agagtcatga atttgcggat gaattgtatc gcacatccga
     6481 cgttcattag taccgcagct gaacatactg gtcatcggcg aaatgcccag ctgtttaatg
     6541 tctttgcgcg catacaggtg attttccaca tccataatca cctgactttt ctcacaatgg
     6601 atagtgaact tataggcacc agtaatgctg gcgctatcga gcaacgcata aacggtaaag
     6661 gtagttgccc ccggttttac cgtatcaaac cagaaggcgg taaagtcggg gaactcttct
     6721 ttactgtcgg tgtaagtgtc gatcgccagg ccgcgggccg acaaaccgta ttgatatgta
     6781 tcatcaacgg cgcggaaata actcgcgccg agaaatgata ctacatcacg gcgcgccagt
     6841 tcgggggctt taaacacgcg aaaaccggca aagccgagat cgctttgccc ttctaattgt
     6901 ttggtatcaa cacctgcatc gttgtatttg aacaactccg ggcgaaagtg aatttcacgc
     6961 gccagatgtg ttgctggatc tacagaaaac atacgaacgc ggcgacggaa tcccattccc
     7021 atatggaaga actgagcgtc cagttgacgg ttctcaacgt tatgccagag cgatttttcg
     7081 gcgtcgtatt gaatactgtt ataagcctgc ggcgtcattg tcgccagcgt gtcaggtaac
     7141 ggacgaggcg caccacgcca cgctgtttgc gctaagtcgt gcgccattga ctgtagaatg
     7201 gagaagtcaa aacgctgggt ttgcccgtcg gcaatatcag aatctgccgc gaatgctgcc
     7261 tgagaaaaaa gagaagcaat gccgctggta ccgcacacgg cggccatagc cattgaacct
     7321 ttaataaatc gtctacgatc catacctgaa agtgagtcct tctggtgttg tgaatggtcc
     7381 cgcgctggtc gttttcagcg gcaagaaagt gcttatttat gaccgctcac tctagacaat
     7441 atttattaat aatccaattg ttgaactgag agaatatgca taaatcggca tttttttatg
     7501 tcgatgggat gagcctgatt cgtttgtaaa atcaggcaaa tttttgcaaa ggcggatggt
     7561 cagaatttaa agcaaaccac agcgacgttt actgcgttct tccgcttctt ggtaaagctg
     7621 gaactcatcc aaaaccggac aacccaacgc ctgctcaaat gcttcgcgac tggcattacc
     7681 gtgactgcgt gtctgcgttt cattacccag atttgactgg aagatccccg ccgcgctaac
     7741 gggcaagaaa tcttcatagg tgatgggttg cgccactacc caaccacgtt caattaaggg
     7801 ctgtggatcg tctccaggat gaatcgcctg acgatgcgcc tcacccgaag gcgtcagacg
     7861 gtaccggaac catgccaacc cttgctgacg cattaaaaac tcactgtcag gaaaagtgcg
     7921 gaaggtttcc tgtaaatgca tttggtgagt gagattatcc tgcccggttc cagcgttacg
     7981 cagaagatca tcatacagtt gtcgcccttt cggcgttaat gccacgccac gctgctcaat
     8041 ttcaccaaag cgcgcggtat gcgtgccctg tttctgcccc gcaaacaaca ccgtctcttc
     8101 cagtgcttta aagctggtct ggcgtagtaa aatcggtacc tcgcggcgcg gcggcccctc
     8161 gatgagaatt ttgggttcaa ttccgcattc aggcatcatc gactgcaccc ggtcaatatc
     8221 cagcgtacgt ggcgtcaggt ggttgatatg gcatccagga aaacagacca catcagcaat
     8281 caaccgatgt tcgttgtgca aggcgcgata ggtttcttca tctaccgttg ctaactagtg
     8341 ccagcgaaac gtttccaggg cttcctgcac aaactcctgt gcctgtgttt cgttaaaacc
     8401 gccctgctgc tcatattcct ctaacagttg tcgacaacgt ggggtgaaga tatcgcgctg
     8461 acgcagaatc tccgccgctt tctggcgcaa aatttcgttc tcgataagct caaggcggag
     8521 taaggatgta aaaacgcgga agggattacg cgccagagaa gcatcatcaa tgggccgaaa
     8581 tgctgtcgaa tgtaccggca cccctgcctg cgagagatcg taatagctca ccgggtacat
     8641 ccccataatg gcaaacatcc gccgaagagt agcaagctct tgtgcagtcc caacgcgaat
     8701 cgccccatga cgttcaacat tcagtcgcgc cagctcgtct gcatttacca ttttttcgtg
     8761 cagttgagga ttgttttcca gcacagccag attcacatca gctaccagtt ccagcagcgt
     8821 gccatattgc ggaacttctt gctggtacat ggctgacatt gcctgcgaaa actgttcccg
     8881 aatctcatcc gccgtgatgc tgttcgccat agtgtctgcc tccagtggtc agtaatctgg
     8941 agtgtaggta acagcattca ctctggcggg aagaatttac aaactgtgat ctcgccgcga
     9001 aaacatcaat attatccatt ttgctgtaac ataattgctt taattgttaa taatatttta
     9061 caatcaagtt atcataatca aacaacttca cttgtcagcg acaccgcttc gtttttaaca
     9121 tcgcttatgg aaaaaaatag tctgtttagt cagcgcatcc gtttgcgcca ccttcataca
     9181 ttcgtagctg tcgcacaaca aggaactttg gggcgcgcag ctgaaaccct taatttgagt
     9241 caacctgcgc tctctaatac attgaatgaa ctggagcagc tgacgggcgc tcgcttgttt
     9301 gagcgtggtc gtcagggggc gcaacttacc ttacccggcg aacaattttt aacgcatgca
     9361 gtcagagttc ttgacgccat caacactgcc ggacagtcgc ttcatcgtaa agaaagtctt
     9421 aataatgatg tcgtcagggt tggtgcacta cctactgcgg cactggggat attaccttcg
     9481 gttataggtc agtttcatca gcaacaaaaa gagacgacct tgcaagttgc gacaatgagt
     9541 aaccctatga ttctggcggg tttaaaaacc ggggaaatcg atatcggcat tggtcggatg
     9601 tcagatcctg aactgatgac cgggcttaat tacgaactgc tgtttcttga atcgttgaag
     9661 ctggttgtcc gccctaatca cccgctactt caggagaacg taacgctaag ccgggtgctg
     9721 gaatggccgg tcgttgtatc accagaaggc actgcgccac gccagcattc agatgcatta
     9781 gtgcagagcc agggctgtaa aattccttcg ggttgtatcg aaacgctgtc tgcttcgcta
     9841 tctcgtcaac ttaccgttga atacgattac gtgtggtttg tcccttctgg cgctgtaaaa
     9901 gacgacctgc gtcatgccac gctggtggcc ctgcctgttc cgggacatgg tgcaggcgaa
     9961 ccgattggaa tactgacccg cgtagatgcg acgttctctt ctggttgcca gttgatgatt
    10021 aacgctattc gaaaatcaat gccgttctga aaggtgaagg gatctgtcga tccctccttg
    10081 aacattttca caccgtagcg aaactaactg gttcacccgc tccgcgaggt tctgccgaca
    10141 cagaatgttt gttcagacgg aatacgttca ccgcctcagt taatcgtgca gcctgttctt
    10201 caagggacac cgccgctgct gaggcctctt ctaccagaga agcattctgt tgcgtcacct
    10261 tatccatttc agaaatcgcc tggctaacct gagttatgcc tctactttgt tcatccgagg
    10321 cggcggcgat ttcctgcatg atatgtgtga cactcgcgac ggcatcaaca atagtgctca
    10381 tggttttccc tgccgttgcc acctcatccg accccaggtc aattaacctg actgattcac
    10441 tgatcaagcc ttcaatctct ttcgctgcct gagcgctgcg gcttgcgagt gtccgtactt
    10501 cgctggcgac aacggcaaat ccacgccctt gctcacccgc tcgcgcggct tcaacggcag
    10561 cattcagtgc cagaatattc gtctggaaag caatactgtt gatgacggcg gtgatctcag
    10621 aaattttctt cgaactcgtg gagatagcgc ccatcgtttt tactacaccg gaaaccgtct
    10681 gcccgccatc gctggcttta atagaagcct cttgcgccag tttgctggca tgatgcgcgt
    10741 tatccgcatt ctgtttcacc gtcgcagtga gttgctccat gctggcggca gtttgttcga
    10801 tagccgccgc ttgttcttcg gtgcgagatg acaggtccgc attgccagct gaaatttcgc
    10861 tggtgccacg ataaatctct tccgcgccct gtcgaacagt ccctactgtc atccccagtg
    10921 aatgctgtat ttgctgtaaa tgacgactta agcgaccgat ttcattacgg cccgccggtt
    10981 catcattcat cgtcagatcg ccactggcga ttttttcaat ccgttgtgcg gcatgttgca
    11041 gtggacgaat gacgatccga cgtagcacca taaatgttat cagcgtcatg accagggcaa
    11101 gcacaaacgc gccaatcatg aacatcccac ccaggcgggt gcgctgatgg gccagttccg
    11161 ccagttggtt ggctctggtg ctacgtatct taacggcttt gttcaaaata tcggtataag
    11221 cattatccag cggtcggatc tgctcacttt catgattgat aatcgcttca aacatgccat
    11281 ttttggcata tttcaacata ggttgcatac ccgtgatata agcctgaaag cgttgattta
    11341 attcagtgtc gagggcttca tcagcaggtg ttttcaccgg tcgattctga taagcacgat
    11401 aaccttgctg cgactgttta atctccgatt cggcttgcgc aatatttcgc ttcattgctt
    11461 ccatttccgc aatacgactc gcagccccgg cctgaatcat attgatccgg gctgaacgca
    11521 aaaaatctga actgttcgct aacccggtcc gaatctcaat ctccgcagta acatcacgct
    11581 gatcgcgatc ggcctgccag aggaaatagc cagccagagc agaacttaat gcgaatagaa
    11641 ctaagatacc gccaagaatg caggcaaata acggaaccag cctgatgtga tgcaaaaaac
    11701 ctaatcgctg tgagggagtt gtattcatta ccgttgtctc tcgtccaggt ttactcctga
    11761 tagaaagtca tcggcacttg acggtaatta cttatgacca aaaacctctt tgcgtcacat
    11821 ttttacaaca attattgcag agaaaattaa tgctattgga atgaagagag taacgaatgg
    11881 ctaaactggc taacccgaat gacgaaaaat tctggaaaac agcacgcaat ttcgtgaagt
    11941 gattattttc gtccctaagg aaacctcata ggatgcctgc gggtgcctga gactttctgt
    12001 tttcaggcta accgggaatc agaaagacga aggccccgac cgatatttct atca
//
LOCUS       DADWTH010000113        11931 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.114, whole genome shotgun sequence.
ACCESSION   DADWTH010000113 DADWTH010000000
VERSION     DADWTH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.114"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..2252)
                     /locus_tag="JNY02_003353"
                     /pseudo
     CDS             complement(1..2252)
                     /locus_tag="JNY02_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2276..2899)
                     /locus_tag="JNY02_003354"
     CDS             complement(2276..2899)
                     /locus_tag="JNY02_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBB9434778.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="JNY02_003355"
     CDS             complement(2896..4584)
                     /locus_tag="JNY02_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBB9434779.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="JNY02_003356"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="JNY02_003356"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBB9434780.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="JNY02_003357"
     CDS             7507..8229
                     /locus_tag="JNY02_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBB9434781.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="JNY02_003358"
                     /pseudo
     CDS             complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="JNY02_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2766 a         3310 c         2942 g         2913 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg aacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagaccg tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttatcc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc taggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctgg ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaactg
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcctcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctaatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag agacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata ataattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccggcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcacccgcta aacaagccgc
     9061 cgctaagcag caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcacc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga aggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccctcacgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gcgaaacatg gcgttagtga
    11641 cacgtaaatc gacggcacca gtatcattag taccagaaga atagattgcg ccactatagc
    11701 cgcctgcgac gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag
    11761 caaatatggc cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca
    11821 ttccagtttc attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc
    11881 caccgctggc attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DADWTH010000114        11785 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.115, whole genome shotgun sequence.
ACCESSION   DADWTH010000114 DADWTH010000000
VERSION     DADWTH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.115"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            202..537
                     /locus_tag="JNY02_003359"
     CDS             202..537
                     /locus_tag="JNY02_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBB9434782.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="JNY02_003360"
     CDS             630..713
                     /locus_tag="JNY02_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434783.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="JNY02_003361"
     CDS             813..1634
                     /locus_tag="JNY02_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBB9434784.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNY02_003362"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNY02_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBB9434785.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNY02_003363"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNY02_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBB9434786.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="JNY02_003364"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="JNY02_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBB9434787.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMEMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNY02_003365"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNY02_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBB9434788.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNY02_003366"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNY02_003366"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBB9434789.1"
                     /translation="MQIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="JNY02_003367"
     CDS             7279..8223
                     /locus_tag="JNY02_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBB9434790.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="JNY02_003368"
     CDS             8409..9791
                     /locus_tag="JNY02_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB9434791.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="JNY02_003369"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="JNY02_003369"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBB9434792.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="JNY02_003370"
     CDS             complement(10547..10882)
                     /locus_tag="JNY02_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBB9434793.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKKKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="JNY02_003371"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="JNY02_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBB9434794.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2995 a         3010 c         2834 g         2946 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggaga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt tgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttctttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaa
//
LOCUS       DADWTH010000115        11595 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.116, whole genome shotgun sequence.
ACCESSION   DADWTH010000115 DADWTH010000000
VERSION     DADWTH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.116"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            139..1824
                     /gene="btsS"
                     /locus_tag="JNY02_003372"
     CDS             139..1824
                     /gene="btsS"
                     /locus_tag="JNY02_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBB9434795.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1821..2540
                     /gene="yehT"
                     /locus_tag="JNY02_003373"
     CDS             1821..2540
                     /gene="yehT"
                     /locus_tag="JNY02_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBB9434796.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2587..3057
                     /locus_tag="JNY02_003374"
     CDS             2587..3057
                     /locus_tag="JNY02_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBB9434797.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3098..3559)
                     /locus_tag="JNY02_003375"
     CDS             complement(3098..3559)
                     /locus_tag="JNY02_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBB9434798.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3684..5687)
                     /locus_tag="JNY02_003376"
     CDS             complement(3684..5687)
                     /locus_tag="JNY02_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBB9434799.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQELVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLNAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(5684..6820)
                     /locus_tag="JNY02_003377"
     CDS             complement(5684..6820)
                     /locus_tag="JNY02_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBB9434800.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(6813..9093)
                     /locus_tag="JNY02_003378"
                     /pseudo
     CDS             complement(6813..9093)
                     /locus_tag="JNY02_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9104..10192)
                     /locus_tag="JNY02_003379"
                     /pseudo
     CDS             complement(9104..10192)
                     /locus_tag="JNY02_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            complement(10734..11141)
                     /locus_tag="JNY02_003380"
     CDS             complement(10734..11141)
                     /locus_tag="JNY02_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434801.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            complement(11202..>11595)
                     /locus_tag="JNY02_003381"
     CDS             complement(11202..>11595)
                     /locus_tag="JNY02_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBB9434802.1"
                     /translation="RSEFAQLFADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKS
                     ATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVY
                     RDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         2800 a         2943 c         3018 g         2834 t
ORIGIN      
        1 ctatagggtg attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat
       61 ttttctgcaa gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg
      121 gaagtgtggt ttgcgggtat gtacgatttt aatctggtgt tgctgctgct tcagcagatg
      181 tgcgtttttt tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg
      241 caggtcacgg ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc
      301 atcatgggca cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata
      361 ggcgcggtaa tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc
      421 ggcggcttac atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc
      481 atcgttgaag gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact
      541 gataaagtct ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa
      601 atgctgatca tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat
      661 attgctgcgc caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg
      721 ctcgataaac gcgcgatgtt tgaaaaatac acttcggctt tttctgccac tgcgctgaaa
      781 gtggcagcct cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa
      841 gtggctcagg tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag
      901 aaattgctgg cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct
      961 tcgacttaca ccttaaaagc gattgaaacc ggtgaagtgg tctacgctga tggcaacgaa
     1021 gtaccttatc gttgctcttt gcatccgcaa tgcaaactgg ggtcgactct ggtaattccg
     1081 ttgcgtggcg aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt
     1141 ttattcagtt caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag
     1201 attcttgccg ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg
     1261 cttcacgccc aagtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg
     1321 atccgccgcg acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc
     1381 aaaaacttaa agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac
     1441 gcttatctgc aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt
     1501 ccgcaagaat tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac
     1561 gccattaaac atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga
     1621 cgtgaggggc aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta
     1681 accaatgcca gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc
     1741 gatgactatg gaataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga
     1801 ctaccatgga gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac
     1861 gggagaacct gcgtatattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt
     1921 caaacgccgt ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg
     1981 atatccagat gccgcgcatc agtggtctgg aaatggtggg gatgcttgac ccggaacatc
     2041 gcccgtatat tgtttttctc actgcgtttg acgaatacgc tattaaagcc tttgaagaac
     2101 atgcctttga ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc
     2161 gattgcgtca ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc
     2221 tgaaatttat cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg
     2281 catttgtcag cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct
     2341 ttaccgaatt gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc
     2401 agtatctggt taacctcgcg catttacagg agattcgtct ggaagataac ggccaggccg
     2461 agttgatttt gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa
     2521 aagaggcgat tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa
     2581 ggcctcatga taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat
     2641 aatgacctgg tgcgtattct ggcgctggat aatgtcgaag ccaccgcgga acagatcgcc
     2701 gtctggctac gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg
     2761 tcattcctca atggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg
     2821 gagccggaac gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg
     2881 ctgaaaaccg atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg
     2941 gaaattacag cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa
     3001 tttttacgtt attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac
     3061 gggtatggcg gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag
     3121 cggctttcat caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag
     3181 aaacgttgat tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag
     3241 tcacgttttc ctgcgcgtag gtatcggtat aggttgattt ttcttcaaca cccgcgatgt
     3301 ttttgtattt ggcgcttaac ggctcaagtg tcttggcagc atcttctttg gtggttgcac
     3361 caatggaggc aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt
     3421 agacataggt aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt
     3481 ctttgtcacc gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca
     3541 gcttattgaa agccttcatg taaaactcca ttttatttaa tcaagaaact ggtgactctc
     3601 accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga actggagtag
     3661 aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg
     3721 caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat
     3781 tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt cgccagcaac
     3841 tctgcctgac taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata
     3901 agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg cagaacgctg
     3961 gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca ggcacgagtc
     4021 tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
     4081 gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaatggca
     4141 taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc accttcgccg
     4201 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
     4261 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
     4321 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgttcaac
     4381 atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg ggtagctaac
     4441 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
     4501 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc ctgttcactt
     4561 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
     4621 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
     4681 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
     4741 ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa tcgactgttt
     4801 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg ggcgtggaag
     4861 gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca gctgcagcgt
     4921 tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg ctggctgatg
     4981 ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt tttgccttct
     5041 tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
     5101 tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc cagtacgaca
     5161 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctgcgggaa
     5221 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg cgcacagaac
     5281 aactcaatgg tgatgctttt agcgaccagc tcctgtgcgc gtttgcgggt ggcatcggga
     5341 agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt tcccgtaggc
     5401 tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg acggcacatg
     5461 ccgctggccc cacaggtgca ctgagcctct ttcagtgcct ggccgttcgc cagctgggta
     5521 cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat ttccgggacg
     5581 ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct taacgccgtc
     5641 agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
     5701 ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg ccgacattaa
     5761 ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg agcgctgcca
     5821 gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc agcaatgatg
     5881 atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca ggttgttcaa
     5941 taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct aattgcactt
     6001 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa
     6061 acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc atcaccgcag
     6121 agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
     6181 gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac aacttgccgt
     6241 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
     6301 ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc tgacgaactt
     6361 cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg cgggcggcag
     6421 ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttcgg
     6481 aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca
     6541 gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tgaatactgt
     6601 tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt tcaccacgct
     6661 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt
     6721 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
     6781 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc
     6841 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa
     6901 tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
     6961 tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc gcgtaaatcg
     7021 ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct gaaaccggcg
     7081 ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc
     7141 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactagcg
     7201 ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag tgccaggaca
     7261 tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga aacgccgggg
     7321 agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca gctatgcaag
     7381 tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca cagcgtgcga
     7441 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
     7501 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
     7561 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
     7621 gctgacgccg cctggctaag acatgtggta atgctgccga ttccacccgc gtccagcata
     7681 tcggcttcta atttatggcg tgcggcttcc tggagtgtgg caccaaagca ggcggcctca
     7741 atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc accgttgcct
     7801 gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag tattgccagc
     7861 cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa ggtaagctcc
     7921 gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg cagaggcggt
     7981 tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
     8041 agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg caacggcgca
     8101 ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt gcgtaacggt
     8161 agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc agccatatcc
     8221 gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
     8281 tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcatt
     8341 cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggg tgagataaca
     8401 acctgtaacg gcatctgcca gcgacggcaa ttctggcgtg ttaattacct gcggacattc
     8461 gcgccacatt tttgccagtg ccggagcgtg ccagccaccg cagacaacta acacgtcgcc
     8521 attattttgc tgcatcgccc atccaatcca gcgggccata aaggcttcgc gctgacgatt
     8581 gagagcatcg ccgggggagt cgccccgcag ttgggcaaaa tagtgcgcca gtgcagaggg
     8641 taatgcagtt tgctggcttt catcttcgaa caagtgatcc cacagggtgt cgctgttatc
     8701 catgcgggtg gcacgcagca gcaaggtctg gctttcctct tgcgtatcag gcgaatcgtc
     8761 ctcttcttca ctctgcgccc agcaaggcaa atcgatgaag taagtttgtg cctgaatacg
     8821 acgatcggct tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc
     8881 acgcccgggg gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg
     8941 gtgggctaaa aacagttcgt ctacccggtc attaaaatca gccgggcctt caatcaacac
     9001 gtatcgtggc cgctggcttt cgattaacga tttcaccagc cgggcgcagg ccggactatg
     9061 atgccggatg ccgacaatta acggctcgct catgcatctt tcctcacggc aggcggtggc
     9121 gggcttgata ataagcctgc cagtgagctt ctttatgtgt ggcgacacgc tgttcgaagt
     9181 aacggcgcag acgagcgcga tcttcttcgt tatctttgac gatggttccg gcaatgcagt
     9241 ccaccaggtc ggctggctca cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg
     9301 cgtgggccac gttcacggct tcagcggtgg acataatcgc tgttagtgta tccattgacg
     9361 ttttcttttc gccgttggca cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg
     9421 cgtcaggcac tttatgcgga ataccgctat gggccagcaa acgcgccgaa gcactggcga
     9481 caagctccag ctcctgggcg aaatccataa tcggaaagac agtttcaaaa tcgaagcgac
     9541 gctttagcgc agcactcatt tcattgacgc cgcgatcgcg ggtattggca gtggcaataa
     9601 tattaaagcc ttctcgcgca tagagctgac tggcttcacc agtgagttcc ggcaccgtca
     9661 tcaccctgtc agaaagcata cctaacagac agtcttgcac ttccagcggt gtacgcgtta
     9721 tctcttcaaa acggacgatt ttgccgtcgc gcattccctg gtaaagcggg gcagggacca
     9781 gcgcttccgt tgatggacca tgattgataa gcagggcgta gttccagccg tacttgattt
     9841 gatcttcggt agtagatgcg ccgccctgga tggttaaccc ggcatcgccg ctaatagagg
     9901 ttgccagtaa ttcagacagg agagattttg cggtgctagg ttcccccacc aacatcaaac
     9961 cgcgtccggt ggcaagagtg actaacatac gttcgacagc ggagggacta atcaccactt
    10021 tacggctaat attttgcgcg ctatcgccaa ggataaaagc acgggccgca ggcaaactta
    10081 actaccaacc gggcgggcaa ggtgcgttat cattttgttt taattttgcc agttcatcgg
    10141 cgtataacac agcagcaggc ggacgctgca gatgattatt ctgcggtgac atatattcat
    10201 ccctgaaatg tattaatgaa ataaacgata ttttacgcgc aataaatgtt tggcgcatta
    10261 gttgtttgag cgaaaaatat ctggaaagca gatcacagta aataaaaaaa cctgctggtg
    10321 aggcaggttc ttttatttac tattttcagg cctaacacca ctgcctgtgc aacatcgtgt
    10381 tccaacgaac gaattataac cacctctgga cagtgttgca attaagataa tgagatttat
    10441 aatcctgact gttgggaagt tgcgccggat cgaacccatt tcaagtcctg gctaccggac
    10501 gggtggtgcc gcaggtagtg ctctgatccg gagtgcaaca tggcgactca acaaacgtaa
    10561 accagtatcg acaggcagtg taagggtaag gtgctggtgt tgtgctcctt aataaggaga
    10621 gcggatggac acgctgacac aaaagttaac cgtgctcatt gctgtactgg agctattagt
    10681 ggctctgtta cggttgattg atttgttgaa gtaacgggca aattgaaaca catctaaggg
    10741 ggaataccgt tccccccttt aataaagcta tactcgtcat acttcaagtt gcatgtgctg
    10801 cgacttgaat tatctagagt atataattct atttgttggt tgaatatatt ctgtagttta
    10861 caattaaaaa ttactattac aggttcaggt gattcgcctc gtaatttttg ctcatcaata
    10921 aactcatctc ttatataaat caacgcatca tttattagcg ggtcaacatc ctctcgatca
    10981 cgttgatgaa atgttggacg tagccggaac cgttccagtc ggtagacgga acctgtaaat
    11041 aattcagagc atttatggat ctcaagtctg aaggaaaacg gatcttcagg tacagggacc
    11101 tcataatcgt aaatagcaac cagctctttt tgcacgatca tcgctttccc cgctggtaag
    11161 tcgtgtagat aacgggcagt cgcatcactg cccgctttgt tttaatggaa aatgacgtca
    11221 ggtgcgctta acgcctcact caaatcaaac acatcaagtt cggcaaaagt gacggattta
    11281 ttgtcacggt acactcgaag agaacggaag ctcattagcg ctttgctatc aacattgtag
    11341 tggttaaaac cgggtgaaaa cttaaggacc agccggtact cacccaggtc atagacaaat
    11401 gcgtcctcat aacctgactc ccagcctttg tatcgcattc ccataagttg cccaacggta
    11461 gcggatttac cttcccagcg agtcaggctg ttactggttg actcgtcagg tgtgagcagc
    11521 accgtgcggc gcgacaactg gcgaaaaggc ggcataattt cgtaatcggc aaaaagctgt
    11581 gcaaattcac tgcgc
//
LOCUS       DADWTH010000116        11555 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.117, whole genome shotgun sequence.
ACCESSION   DADWTH010000116 DADWTH010000000
VERSION     DADWTH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.117"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JNY02_003382"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JNY02_003382"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNY02_003383"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNY02_003383"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBB9434803.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNY02_003384"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNY02_003384"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBB9434804.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNY02_003385"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNY02_003385"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBB9434805.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /gene="garP"
                     /locus_tag="JNY02_003386"
     CDS             complement(3367..4701)
                     /gene="garP"
                     /locus_tag="JNY02_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBB9434806.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDSAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="JNY02_003387"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="JNY02_003387"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBB9434807.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPTSELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNY02_003388"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNY02_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBB9434808.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="JNY02_003389"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="JNY02_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="JNY02_003390"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="JNY02_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBB9434809.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="JNY02_003391"
     CDS             9047..9826
                     /locus_tag="JNY02_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBB9434810.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="JNY02_003392"
     CDS             9816..10625
                     /locus_tag="JNY02_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBB9434811.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..11146
                     /locus_tag="JNY02_003393"
     CDS             10712..11146
                     /locus_tag="JNY02_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB9434812.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11143..>11555
                     /locus_tag="JNY02_003394"
     CDS             11143..>11555
                     /locus_tag="JNY02_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBB9434813.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2755 a         3101 c         2949 g         2750 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggcgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ccaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg ctgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaagtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccga cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactacataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactcgaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcaggtcag aaaactatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtatcaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cctttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg
    10921 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac
    10981 cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga
    11041 agaattccgt gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga
    11101 gttaggccgc tgccatgaag aatatccggt cgaggaagga atatgacaca cgttctgcgc
    11161 gccagaaggc tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac
    11221 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    11281 ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat
    11341 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    11401 tggttaccga ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt
    11461 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    11521 ccgtacttca cgccgcagaa taaaggcgcg catcc
//
LOCUS       DADWTH010000117        11439 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.118, whole genome shotgun sequence.
ACCESSION   DADWTH010000117 DADWTH010000000
VERSION     DADWTH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.118"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            9..461
                     /locus_tag="JNY02_003395"
     CDS             9..461
                     /locus_tag="JNY02_003395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB9434814.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="JNY02_003396"
                     /pseudo
     CDS             707..1751
                     /locus_tag="JNY02_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1813..2427
                     /locus_tag="JNY02_003397"
                     /pseudo
     CDS             1813..2427
                     /locus_tag="JNY02_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JNY02_003398"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JNY02_003398"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBB9434815.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="JNY02_003399"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="JNY02_003399"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBB9434816.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="JNY02_003400"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="JNY02_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBB9434817.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /gene="crl"
                     /locus_tag="JNY02_003401"
     CDS             6055..6456
                     /gene="crl"
                     /locus_tag="JNY02_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBB9434818.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JNY02_003402"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JNY02_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBB9434819.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="JNY02_003403"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="JNY02_003403"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBB9434820.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="JNY02_003404"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="JNY02_003404"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBB9434821.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10321..10396
                     /locus_tag="JNY02_003405"
     tRNA            10321..10396
                     /locus_tag="JNY02_003405"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10354..10356,aa:Thr,seq:cgt)
     gene            complement(10411..>11439)
                     /locus_tag="JNY02_003406"
     CDS             complement(10411..>11439)
                     /locus_tag="JNY02_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBB9434822.1"
                     /translation="AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWML
                     HFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTK
                     TRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPV
                     VMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQ
                     GLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWA
                     SWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEE
                     NVVYLNAR"
BASE COUNT         2746 a         2838 c         2946 g         2909 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggtccat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtag
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcgtagctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gtgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtttggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat taccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctgaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact
    10381 cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc
    10441 ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc
    10501 ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc
    10561 tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt
    10621 atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca
    10681 tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg
    10741 gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa
    10801 tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc
    10861 agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac
    10921 gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc
    10981 aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt
    11041 aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt
    11101 gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg
    11161 tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt
    11221 caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat
    11281 gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc
    11341 atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag
    11401 ctttttcggt gattccccga gattttttac tctccacgc
//
LOCUS       DADWTH010000118        11435 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.119, whole genome shotgun sequence.
ACCESSION   DADWTH010000118 DADWTH010000000
VERSION     DADWTH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.119"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="JNY02_003407"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="JNY02_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBB9434823.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="JNY02_003408"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="JNY02_003408"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBB9434824.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="JNY02_003409"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="JNY02_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBB9434825.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="JNY02_003410"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="JNY02_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBB9434826.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="JNY02_003411"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="JNY02_003411"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBB9434827.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="JNY02_003412"
     CDS             6426..7055
                     /locus_tag="JNY02_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBB9434828.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="JNY02_003413"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="JNY02_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBB9434829.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="JNY02_003414"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="JNY02_003414"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB9434830.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="JNY02_003415"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="JNY02_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBB9434831.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         3003 a         2936 c         2763 g         2733 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg acttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcta ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tccgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aattgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcac ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataaccg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcctggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctggct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggttat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaa
//
LOCUS       DADWTH010000119        11269 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.120, whole genome shotgun sequence.
ACCESSION   DADWTH010000119 DADWTH010000000
VERSION     DADWTH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.120"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNY02_003416"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNY02_003416"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBB9434832.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="JNY02_003417"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="JNY02_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBB9434833.1"
                     /translation="MAAAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="JNY02_003418"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="JNY02_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBB9434834.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="JNY02_003419"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="JNY02_003419"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBB9434835.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4801..5802
                     /gene="add"
                     /locus_tag="JNY02_003420"
     CDS             4801..5802
                     /gene="add"
                     /locus_tag="JNY02_003420"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBB9434836.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5838..6878)
                     /locus_tag="JNY02_003421"
     CDS             complement(5838..6878)
                     /locus_tag="JNY02_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB9434837.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7121..7246
                     /gene="blr"
                     /locus_tag="JNY02_003422"
     CDS             7121..7246
                     /gene="blr"
                     /locus_tag="JNY02_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBB9434838.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7519..7734
                     /gene="ydgT"
                     /locus_tag="JNY02_003423"
     CDS             7519..7734
                     /gene="ydgT"
                     /locus_tag="JNY02_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBB9434839.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7820..8260
                     /locus_tag="JNY02_003424"
     CDS             7820..8260
                     /locus_tag="JNY02_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBB9434840.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8337..8918
                     /gene="rsxA"
                     /locus_tag="JNY02_003425"
     CDS             8337..8918
                     /gene="rsxA"
                     /locus_tag="JNY02_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBB9434841.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8918..9496
                     /gene="rsxB"
                     /locus_tag="JNY02_003426"
     CDS             8918..9496
                     /gene="rsxB"
                     /locus_tag="JNY02_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBB9434842.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9489..>11269
                     /gene="rsxC"
                     /locus_tag="JNY02_003427"
     CDS             9489..>11269
                     /gene="rsxC"
                     /locus_tag="JNY02_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBB9434843.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAA"
BASE COUNT         2621 a         2912 c         2841 g         2895 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagtttact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggcagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttacc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggctcg gggagactgg
     4201 gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa tcgttgcgca atcgtggatt
     4741 tttaccctgc tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc
     4801 atgattgata ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt
     4861 ccccagacca ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctt
     4921 gaaacactga ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg
     4981 accaaacttg actggggcgt taaagttctc gcctctcttg atgcctgccg ccgcgtggca
     5041 tttgaaaaca ttgaagatgc agcccgtaac ggcctgcgct atgtcgagct gcgtttttca
     5101 ccaggctaca tggcaatggc acataagctg cctgtagcgg gtgttgtcga agcggtgatc
     5161 gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg
     5221 agccgaacct tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt
     5281 gaccagatta ccgcacttga tttagctggt gatgaacttg gattcccggg aagtctgttc
     5341 ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa
     5401 gctgccgggc cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga
     5461 catggcgtaa aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt
     5521 ggtattgaat cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca
     5581 catccgctga aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc
     5641 ggcgtacagg gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta
     5701 tcccgtgagc aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgca
     5761 gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt aacaaaaatg gatggtgcaa
     5821 attgcaccat ccatttttca cgcaaggcac aacgtcgcgc gatgtttggc ggattcgatg
     5881 cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca
     5941 tcgccattta acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc
     6001 aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata
     6061 tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga
     6121 tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca
     6181 gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg
     6241 tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg
     6301 accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa atcgtaccag
     6361 ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg
     6421 tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt
     6481 agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac acgccccagg
     6541 ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca
     6601 acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg ggtatcgttg
     6661 ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag
     6721 acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc
     6781 gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa
     6841 ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta
     6901 tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc tgccagcaat
     6961 aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa
     7021 tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc
     7081 cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc ttattgaatt
     7141 aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga
     7201 caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc tgcacttgtg
     7261 gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa accaggaatt
     7321 tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc
     7381 agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac
     7441 aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct ttggtattaa
     7501 ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt
     7561 tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag
     7621 gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg
     7681 cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca ataaagaaag
     7741 tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg cctgtttgcg
     7801 tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct
     7861 tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta
     7921 taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac
     7981 acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg
     8041 gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct
     8101 gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg aagttggcat
     8161 agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa
     8221 acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt
     8281 tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga
     8341 ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt
     8401 ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg
     8461 ggctggcaac aacgtttgtg atgacgctga cgtctatttg cgcctggctt atcgatacgt
     8521 ggattttgat cccacttaat ctgatttacc tacgcaccct ggcatttatt ctggtgattg
     8581 ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc
     8641 tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc gtggcgttgc
     8701 tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt tccgccgctg
     8761 tcggtttctc gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt gctgtggctg
     8821 atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc
     8881 tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt
     8941 tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg cctcccgccg
     9001 ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca
     9061 gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg
     9121 tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt
     9181 gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat
     9241 ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc aggcttgtcc
     9301 ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg atctctgtac
     9361 gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc
     9421 agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt
     9481 ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg
     9541 gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg cgccaggtac
     9601 cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa ggtgagttgt
     9661 gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa
     9721 tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg
     9781 ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt gaagactgct
     9841 ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag ttaatcgagc
     9901 gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca ggcgttaaat
     9961 tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt
    10021 acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtattc
    10081 gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa gataacaaac
    10141 cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt tctctgcggg
    10201 tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga
    10261 agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca
    10321 ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgttgtaa
    10381 ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggaacgccag
    10441 tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg
    10501 gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta aagattacca
    10561 actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagctgca
    10621 tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa cagttgtact
    10681 ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc gactgcattg
    10741 aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa tatttccgtc
    10801 aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag
    10861 cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agctgctcgc cttgaacgac
    10921 ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg
    10981 cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac
    11041 gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga
    11101 aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca cgtaaaactg
    11161 ccgttgaagc agctatcgcc cgctccaaag cgcgcaagct ggaacagcaa caggctaatg
    11221 cggaaccaga agaacaggtc gatccgcgca aagccgccgt cgaagccgc
//
LOCUS       DADWTH010000120        11093 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.121, whole genome shotgun sequence.
ACCESSION   DADWTH010000120 DADWTH010000000
VERSION     DADWTH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.121"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            215..2920
                     /gene="malT"
                     /locus_tag="JNY02_003428"
     CDS             215..2920
                     /gene="malT"
                     /locus_tag="JNY02_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBB9434844.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWNLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALVQQTEQIARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2963..3840)
                     /gene="rtcA"
                     /locus_tag="JNY02_003429"
                     /pseudo
     CDS             complement(2963..3840)
                     /gene="rtcA"
                     /locus_tag="JNY02_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            complement(3844..5070)
                     /gene="rtcB"
                     /locus_tag="JNY02_003430"
     CDS             complement(3844..5070)
                     /gene="rtcB"
                     /locus_tag="JNY02_003430"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBB9434845.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5259..6857
                     /gene="rtcR"
                     /locus_tag="JNY02_003431"
     CDS             5259..6857
                     /gene="rtcR"
                     /locus_tag="JNY02_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBB9434846.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKAAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6839..7597)
                     /locus_tag="JNY02_003432"
     CDS             complement(6839..7597)
                     /locus_tag="JNY02_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBB9434847.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="JNY02_003433"
     CDS             complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="JNY02_003433"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBB9434848.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="JNY02_003434"
     CDS             complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="JNY02_003434"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBB9434849.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9005..10510
                     /gene="glpD"
                     /locus_tag="JNY02_003435"
     CDS             9005..10510
                     /gene="glpD"
                     /locus_tag="JNY02_003435"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBB9434850.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10779..11009)
                     /locus_tag="JNY02_003436"
                     /pseudo
     CDS             complement(10779..11009)
                     /locus_tag="JNY02_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         2679 a         3036 c         2841 g         2537 t
ORIGIN      
        1 gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg tgagttgtta
       61 ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac gtcatcgctt
      121 gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca aaaaaaataa
      181 tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa aactaagtcg
      241 tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc
      301 gaacaacttc cggctggcgc tgatcacgag tcctgcgggc tacggaaaga ccaccctcat
      361 ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggatg aaggtgataa
      421 ccagcaagag cgtttcgcca gctatctcat tgccgccgtg cagcaggcaa ccaacggtca
      481 ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat gccagcctga cgtcactctt
      541 cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg tcatcgatga
      601 ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta ttcgccatca
      661 accagaaaat ctcaccctgg tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa
      721 tctgcgtgtt cgtgaccaac tgctggaaat tggtagtcag caactggcat ttacccatca
      781 ggaagcgaag cagttttttg attgccgtct gtcatcgccg attgaagccg cagaaagcag
      841 tcggatttgc gatgacgttt ccggttgggc gacggcacta cagctaatcg ccctctccgc
      901 ccggcagaat acccactcag cccataagtc ggcacgccgc ctggcgggaa tcaatgccag
      961 ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg caacgcgcca
     1021 ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gccctcatca cccgtgtgac
     1081 cggcgaagaa aacgggcaaa tgcgtctcga agagattgag cgtcaggggc tgtttttaca
     1141 gcggatggat gataccggcg agtggttctg ctatcacccg ctgtttggca acttcctgcg
     1201 ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg ccgccgcaga
     1261 aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg cggcaggcga
     1321 tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg aatttgttca accatagcga
     1381 actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc
     1441 gcagttagtg ttattgcagg cgtggctaat gcaaagccaa catcgctatg gcgaagttaa
     1501 caccctgcta gcccgtgctg aacatgaaat caaggacatc agagaaggca ccatgcacgc
     1561 agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg atgaagcgga
     1621 acggctggca aaactggcac tggaagagct gccgccgggc tggttctata gccgcattgt
     1681 ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc gaattgaccc gctcactggc
     1741 gctagtgcag caaaccgaac agatagcacg ccagcacgac gtctggcact acgccttatg
     1801 gagtttaatt cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga
     1861 aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat ctggaacagc tgccaatgca
     1921 tgagtttctg gtgcgcatcc gagcacaact gttatgggcc tgggcacggc tggatgaagc
     1981 cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct tatcagccac agcaacagct
     2041 tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag ataacgcccg
     2101 tagccagttg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca gcgactggat
     2161 ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg accggcgata aagccgccgc
     2221 tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact tcctgcaagg
     2281 tcaatggcgc aacattgccc gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat
     2341 tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg
     2401 taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg acgcccagcg
     2461 cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga tttatcagtc attttgtcat
     2521 cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt caacttaata cgctgccgga
     2581 actggaacag catcgcgcgc agcgtattct gcgagaaatc aatcaacatc atcggcataa
     2641 attcgcccat ttcgatgaga atttcgttga acgtctgcta aatcatccgg aagtgccaga
     2701 actaatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc tgatctactc
     2761 cggttacagc aatgaacaaa ttgcgggcga actggaagtg gcggcaacca ccatcaagac
     2821 gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca
     2881 cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga gtttagccgg ataatgcatt
     2941 ggatccggtt tacatcttta catcattcaa tactcacccg cgttacgcca tctgtttcta
     3001 tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc gatattggtc agcagatggc
     3061 atgaaggatg ggcgaccgta aattctcccg cgcccgccag cgccatcggc agtaccagtt
     3121 ggtcggcgag atattccccc accgccgctg ggcttgccag gtagcgtttc acctctttca
     3181 ctaactgcgc ggctaccacc tcggcactga cgcgcttttc accgacgacg aaaaagcgtt
     3241 cagtgatatt ttcactttcg acttccagcg agacggtatt acccggcccc tggtcgcgcg
     3301 gcaggttatg aatattctgt tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac
     3361 gctcagcaac atggcgcggc acaccagcta atagaacttc tccacgcatc tgcacaatgt
     3421 tcccgcgctc gccaagttgc aaggtgctaa acgatgccac gggcgagact tccgttgcca
     3481 ccacaccgcc tccggcagga taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc
     3541 ctattttcgc cagcagcggc tccagcaccc ggcggataaa atccgcaggc ggcgccgacg
     3601 ggttatcggt gccgccgctc acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg
     3661 gcagcaccgt ttgcagcacc agcgtacaac ttccggcgct accgatagca aagcggtaat
     3721 cgccgccgcg cacggtgcca ggccggaaga ccagacgctg cgatcccagc tcagcgccga
     3781 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
     3841 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
     3901 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
     3961 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
     4021 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
     4081 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
     4141 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
     4201 aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
     4261 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
     4321 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
     4381 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
     4441 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
     4501 tccacgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
     4561 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
     4621 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
     4681 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
     4741 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
     4801 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
     4861 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
     4921 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
     4981 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
     5041 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
     5101 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaatttt ttaatttatt
     5161 gtttctaaaa agattatatc tttatgtccg taaacggaga tttcccgcaa agccgattta
     5221 ccgataatga aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg
     5281 gctttgtcgg taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc
     5341 gtccgtcact ctgtatatgc cagcaagaat cgttggtcat cgatcgactg gaattattgc
     5401 acgatgcccg ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc
     5461 cagaaacaga agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg
     5521 tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact
     5581 atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg
     5641 aagcgcgtta tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc
     5701 cgcgcggccc aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg
     5761 ccagccgctt tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca
     5821 cgcgtaaccc ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc
     5881 gcgcgccgat tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca
     5941 tcttcgagtt aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg
     6001 ccaccctgcg cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa gccgcgttta
     6061 ccggggcgcg ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc
     6121 ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag
     6181 agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg
     6241 ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt
     6301 acgcgcggat caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata
     6361 ttgaaccgaa cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc
     6421 gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggctacat
     6481 ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta
     6541 gcggacgcat cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc
     6601 aggagagccg cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc
     6661 tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt
     6721 ctgctgccgg acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg
     6781 cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaggcc gtgcaggatc
     6841 agcacagctc cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg
     6901 gggcgtcggt gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac
     6961 ggccaaattt tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc
     7021 gcttggtacg aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc
     7081 tgatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc
     7141 caatgatccc gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt
     7201 cttttaccat caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt
     7261 gattgagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg
     7321 agccattggg gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg
     7381 tggctttacg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat
     7441 gatggcgcag gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg
     7501 ggctgacgga gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca
     7561 gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt
     7621 cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg
     7681 atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag
     7741 atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg
     7801 ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac
     7861 ggcccgctga atttttgctg cacatagccg cttaacaggg cgctgataag cgtaatgaca
     7921 attagcttac cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc
     7981 aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag
     8041 aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg
     8101 ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc
     8161 ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg
     8221 ccggtatggc ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga
     8281 aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg
     8341 ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac
     8401 gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat
     8461 tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact
     8521 gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca
     8581 gcagatactg cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag
     8641 tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct
     8701 gcaccgcatg ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt
     8761 tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc
     8821 tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat
     8881 aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg
     8941 attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg
     9001 cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc
     9061 ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg
     9121 cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta
     9181 tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc
     9241 gcatatcgcc ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg
     9301 gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg
     9361 atcaattggt ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt
     9421 cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt
     9481 ggtgcgtaaa ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa
     9541 cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc
     9601 gcgcggcctg gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca
     9661 tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt
     9721 gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat
     9781 cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc
     9841 gaaagcagtg aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca
     9901 ctttaaaaag cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct
     9961 gtgtgatgat gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca
    10021 tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg
    10081 aaaactggca gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc
    10141 gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc
    10201 cgctcgcctg cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacgcgcg
    10261 cacttacggc agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg
    10321 ggaagatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg
    10381 ggtccgccgc accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc
    10441 ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct
    10501 ggcgtcgtaa attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga
    10561 caaacacaaa actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat
    10621 tgaatttgca tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa
    10681 gcgcactttg tcaaaaatct aacctacttt ttaattcagg gaattaccgc aaagcccacg
    10741 ccatcatacc caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc
    10801 attaatgcgc ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag
    10861 agtaaattgc aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct
    10921 gcaaaaaatt gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc
    10981 cattttctat tttgcattat tctttccatt ctttctgaat ggcgaaatta tactcccctt
    11041 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt tgg
//
LOCUS       DADWTH010000121        10815 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.123, whole genome shotgun sequence.
ACCESSION   DADWTH010000121 DADWTH010000000
VERSION     DADWTH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.123"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            93..1178
                     /locus_tag="JNY02_003437"
     CDS             93..1178
                     /locus_tag="JNY02_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBB9434851.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1249..1857
                     /locus_tag="JNY02_003438"
     CDS             1249..1857
                     /locus_tag="JNY02_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBB9434852.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1955..3346
                     /gene="selA"
                     /locus_tag="JNY02_003439"
     CDS             1955..3346
                     /gene="selA"
                     /locus_tag="JNY02_003439"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBB9434853.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3343..5187
                     /gene="selB"
                     /locus_tag="JNY02_003440"
     CDS             3343..5187
                     /gene="selB"
                     /locus_tag="JNY02_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBB9434854.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5377..6528
                     /gene="yiaY"
                     /locus_tag="JNY02_003441"
     CDS             5377..6528
                     /gene="yiaY"
                     /locus_tag="JNY02_003441"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBB9434855.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6693..8231
                     /gene="aldB"
                     /locus_tag="JNY02_003442"
     CDS             6693..8231
                     /gene="aldB"
                     /locus_tag="JNY02_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBB9434856.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8450..8593)
                     /locus_tag="JNY02_003443"
     CDS             complement(8450..8593)
                     /locus_tag="JNY02_003443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434857.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            8776..9099
                     /locus_tag="JNY02_003444"
     CDS             8776..9099
                     /locus_tag="JNY02_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBB9434858.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9105..10241
                     /locus_tag="JNY02_003445"
     CDS             9105..10241
                     /locus_tag="JNY02_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBB9434859.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQVR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10238..>10815)
                     /locus_tag="JNY02_003446"
     CDS             complement(10238..>10815)
                     /locus_tag="JNY02_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9434860.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2659 c         2977 g         2602 t
ORIGIN      
        1 atcgccgcac tttctgaccg aatttcccgg ctggagcatc aactcgccgc cgagaaaaag
       61 actgactatt ccacgttccc ggagatctaa ttatggatct attgattgtt ttaacttacg
      121 tggcgctggc gacggcggcg ctgggaggcg tgtttctggt gagtggtttg attttgttga
      181 tgaactacaa ccacccttac acttttaccg cgcaaaaggc agtgatagcg atccctatca
      241 cgccacaggt gacgggaatt gttactgaag tcactgacaa gaataatcag cttattcaaa
      301 agggcgaggt gctttttaag ctcgacccgg ttcgttacca ggcgcgagtt gacagacttc
      361 aggctgacct gatgacggcg acgcataata taaagacgct gcgtgcgcag ctcactgaag
      421 cgcaggccaa caccacccag gtttcagcgg agcgcgaccg tctgtttaaa aattatcaac
      481 gttacctgaa aggcagccag gcggcggtga atccgttctc ggaacgtgac atcgacgatg
      541 cgcggcaaaa tttcctcgcg caggatgcgt tagtgaaagg ctcggtggcg gagcaggcgc
      601 agatccagag tcagctcgac agtatggtca acggcgagca atcgcagatt gtgagcttaa
      661 gagcgcaact tactgaagca aaatataatc ttgagcagac tgtcattcgc gcaccaagca
      721 atggctacgt cactcaggta ctgatccgcc caggtacata cgcagctgcc ttgccgctgc
      781 gtccggtgat ggtcttcatc cccgagcaaa aacggcaaat tgtcgcccaa tttcggcaaa
      841 actcgctgtt acgtctgaaa cctggcgatg atgcggaagt ggtgtttaac gcgctacctg
      901 ggcaggtgtt ccacggcaaa ttgactagta ttttacctgt cgtgccaggc ggttcttatc
      961 aggcgcaggg ggtattgcaa tcattaacgg tcgttcccgg cacggacggt gtgctgggaa
     1021 ccattgaact ggaccctaac gatgatatcg atgccttacc cgacggcatc tacgcccagg
     1081 tggcggttta ctccgaccat ttcagccatg tttcggtgat gcggaaagtg ctgctaagaa
     1141 tgaccagttg gatgcattat ctttatttgg atcattgaga cttatatcac ctgtgttttg
     1201 cgtgagcctg agccatactg atatttttcc tcgcacaaag gactcggcat gaaactcgtc
     1261 ggtagctaca ccagcccgtt tgtacgcaaa ctttctattc tgttgttaga aaagggcata
     1321 actttcgaat ttattaatga actgccctat aacgcggata acggcgtggc gcaatttaac
     1381 ccgttaggga aagtgccggt gctggtgacc gaagagggcg aatgctggtt tgattcgccg
     1441 atcatcgctg aatatattga gttaatgaat gtcgctccgg cgatgttgcc gcgcgatccg
     1501 ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg atggcattat ggatgccggg
     1561 ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc agtctgaaga tgaattgtta
     1621 cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg aaggatatct ggtcgatggc
     1681 acactcaaaa ccgatacggt caatctggcg actatcgcca ttgcctgtgc tgtcggatat
     1741 ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc gcccacactt agtcaaactg
     1801 gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg aaccgccaaa ggcttgatgc
     1861 gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg gttatgttaa acgcccttct
     1921 ccgtgtgaga gggccttgat cagccaggtt tcctatgaca accgaaacgc gttccctcta
     1981 tagtcaactt ccggctattg atcgcttatt acgcgatagc tccttccttt ctttgcgtga
     2041 tacttatggt cacacccgcg tggtggaatt gttgcgtcag atgctcgacg aagcgcgaga
     2101 agtgattcgt ggcagccaga cgctgcctgc gtggtgtgaa aactgggcgc aagaagtcga
     2161 tgcccggttg acgaaagagg cgcagagcgc gctgcgtccg gtgatcaacc tgacgggaac
     2221 cgtgctgcat accaaccttg ggcgagcttt acaggcggaa gccgcggtgg aagccgttgc
     2281 gcaggctatg cgttcgccag tgaccctcga gtacgatctg gacgacgccg gacgcggaca
     2341 tcgcgatcgg gcgctggcgc agctgctgtg ccgtattacg ggggcggaag atgcctgtat
     2401 cgtcaataac aatgcggcgg cggtgttatt gatgttggcg gccactgcca gcgggaaaga
     2461 ggtggtggta tctcgcggcg aactggtgga gattggcggc gcgtttcgta ttcccgatgt
     2521 tatgcgtcag gcaggctgca ccctacacga agtagggacc accaaccgca cgcacgcgaa
     2581 tgattatcgt caggcggtga atgaaaatac cgcactgttg atgaaagtac ataccagtaa
     2641 ctacagcatt caggggttca ccaaagcgat agacgaagct gaactggtgg cgctcggcaa
     2701 agagctggat gttccagtgg tgactgattt aggcagtggc tcgctggtcg atcttagcca
     2761 gtacggtttg ccgaaagagc caatgccgca ggagttgatt gcggcgggcg tcagtctggt
     2821 gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca gggattatcg ttggtaaaaa
     2881 agagatgatc gcccgcctgc aaagccaccc gctgaagcgt gcattacgcg cggataaaat
     2941 gaccctcgcg gcgctggaag ccacgttgcg tctttattta caccctgaag ctctgagtga
     3001 aaaattaccg accctgcgcc tgcttacccg cagcgcagag gtcattcaaa tccaggcaca
     3061 acgtttacag gctccccttg tcgcacatta cggcgcggag tttgcggtac aggttatgcc
     3121 atgtctttcg cagattggca gtggttcgct gccggttgat cgcctgccga gcgcggcatt
     3181 aacgtttaca ccccatgatg gacgcggtag ccaccttgag tcattagccg cccgctggcg
     3241 tgaattgcca gtgccggtga ttggtcgtat ttatgacgga cgattgtggc tggatttacg
     3301 ctgccttgaa gatgagcaac ggtttttgga gatgttgttg aaatgattat tgcgactgcc
     3361 ggacacgttg accacggcaa aacaacctta ttgcaggcga ttactggcgt aaatgctgac
     3421 cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc tcggctatgc ctactggccg
     3481 cagccggatg gtcgcgtgcc tggttttatc gacgttcccg gtcatgaaaa gtttctttcc
     3541 aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt tggtggtggc gtgcgatgac
     3601 ggcgtgatgg cacagacccg tgagcatctg gcgattttgc agctgaccgg taacccgatg
     3661 ctgacagtgg cgctgaccaa agccgatcgt gtggacgaag cgcgtgttga tgaggttgaa
     3721 cgccaggtaa aggaggttct gcgggaatac ggttttgctg aggcaaaact gtttatcacc
     3781 gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc atctgcttca gttgccggaa
     3841 cgcgagcacg ccagccaaca tagtttccgc ctcgcgattg accgcgcatt taccgtaaaa
     3901 ggtgccgggc tggtcgtcac cggtacggcg ttaagcgggg aagtgaaggt aggcgattca
     3961 ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag
     4021 ccaacagaaa ccgccaatgc cgggcagcgt atcgcgcttg acatcgcggg tgatgcggaa
     4081 aaagagcaga ttaaccgtgg cgactggctg cttgccgatg tgccgccaga gccgttcaca
     4141 cgggtgattg tcgagcttca aacccatgca ccgctgaccc agtggcagcc gctgcatatt
     4201 caccacgccg ccagccacgt cacgggacgc gtttcactgc tggaagataa ccttgctgaa
     4261 ctggtcttcg acaccccgtt atggctggca gataacgacc gcctggtatt gcgcgatatc
     4321 tctgcccgca acacgctggc cggagcgcgc gtcgtgatgc ttaacccgcc gcgtcgcggt
     4381 aaacgtaaac cagagtatct gcaatggctg gcttccctcg ccagggcaca gaatgatagc
     4441 gaagcgttag ccgttcacct gcaacgcggc gcggttaatc ttcctgattt cgcctgggcg
     4501 cgccagctca acggcgaagg gatgcgcgaa ctgctgcaac agcctggcta tattcaggct
     4561 ggttatagct tgttgaatgc gccggttgcc gctcgctggc aacggaaaat tctcgacaca
     4621 ttagcgactt atcatgagca acatcgcgat gaacctgggc ctgggcgcga acgtctgcga
     4681 cgtatggcgt tgccaatgga agatgaagcg ctagtactgt tgctgattga aaagatgcgc
     4741 gaaagcggcg acatcctcag tcatcacggt tggctgcatc tgccggatca caaagcgggc
     4801 ttcagcgaag agcagcaggc catctggcaa aaagcagagc cgctgtttgg tgacgagccg
     4861 tggtgggtgc gtgatctggc aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg
     4921 ctacgccagg cggcgcagca aggaataatt accgcgatcg tcaaagatcg ttattaccgt
     4981 aacgatcgga ttgtcgaatt tgccaatatg atccgcgatc tcgatcagga gtgtggttca
     5041 acctgcgcgg cggatttccg cgatcgctta ggcgtaggcc gaaagctggc aattcagatt
     5101 ctggaatatt ttgaccgcat tggctttacg cgtcgtcgtg gaaatgatca tttattgcgc
     5161 gacgcattat tatttccgga aagataagga aatgattaat taatttttaa aataaattaa
     5221 tacaaatttc ttatgatttt aaataaagca cattgtttaa tgaatacaat gtgcttttta
     5281 ttagattaat tttgataatt aataataatg atcatgtttt cgaatgaaaa tcctcagtaa
     5341 gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac tttctttatt
     5401 ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat gatggcagat
     5461 tatggattta cccgtacctt aattgtcact gacaatatgt taacgaaatt aggtatggcg
     5521 ggtgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta tgatggcacc
     5581 caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa agaaaataat
     5641 tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa aggtattgcg
     5701 ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg ctctgcaaaa
     5761 ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga aatgacccgt
     5821 ttctgcatca tcactgacga agcgcgtcat aacaaaatgg cgattgttga taagcacgtg
     5881 actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa gtcactgacc
     5941 gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc tattgccgcc
     6001 acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga aaacctgccg
     6061 ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta tgcccagttc
     6121 cttgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat ggcgcaccag
     6181 ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttctgct gccgcatgtt
     6241 caggtattca acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc tgcaatgggc
     6301 gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa cgccatccgt
     6361 gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt gaaagaagaa
     6421 gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggctttac taatccgatc
     6481 caggcaactc acgaagaaat tgtggtgatt tatcgcgccg caatgtaatc atcattccaa
     6541 caacggctgg caaattgtca gccgcttttt caactatctc tgtaatcctt gcccgtaaat
     6601 tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt cacgtctacc
     6661 cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct tcagcacaga
     6721 ttaagcccgg cgagtatggt ttccccatca agttaaaagc ccgctacgac aactttattg
     6781 gcggtgaatg ggtagcccct gccgatggtg agtattacca gaacctgacg ccggtgaccg
     6841 ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg
     6901 ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc gcggcgattc
     6961 tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca gctgaaacct
     7021 gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg gcgattgacc
     7081 atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt gaagttgata
     7141 gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag attatcccgt
     7201 ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg gcgggcaact
     7261 gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta atggaaattg
     7321 tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt ggggaaattg
     7381 gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag
     7441 tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg ctggagttgg
     7501 gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat gcctttttcg
     7561 ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt tgcacctgtc
     7621 cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt tatggaacgc gccatccgcc
     7681 gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg ggcgcgcagg
     7741 tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa aaagagggcg
     7801 ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa gacggctact
     7861 acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag gaggagattt
     7921 ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg gagctggcga
     7981 acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat ctggcctata
     8041 agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac gcttacccgg
     8101 cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc cacaagatga
     8161 tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat aaaccgttgg
     8221 ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg cgacgcttac
     8281 gcgtcttatc atgcctacgg gaacctgacc gtaggccgga taaggcgtac acgccgcatc
     8341 cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg cgcaataacc
     8401 cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg
     8461 tttcgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg aggccaatca
     8521 gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg gatgttttac
     8581 tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa acctcgtcct
     8641 gttgaaaaac cagacagcac atttcagaaa gtatataaaa aacaatatgt cgttatataa
     8701 ataaggaaat tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata
     8761 ttctttgagg gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt
     8821 ctggtgatat tctatgggat tattatttta catgatattc cctacctgat ttctaaaaaa
     8881 cgtaatcatc ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgctg
     8941 catgttatct ggccattttt gtggatttgg gccacgcttt accgcccgga gcggggatgg
     9001 ggaatgcaaa tccatgactc ctccgttgtg caactacaac agcgtattgc cgggctggag
     9061 aaacagctcg ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta
     9121 ttttgaccta tgtggctttt gcatgggcaa tgtttaagat cttcaaaatt cctgtaaata
     9181 aatggaccat ttccacagcg gccctgggtg gcatatttat tgtcagtggt ttaattctgt
     9241 taatgaacta taaccatccg tataccttca aggcgcaaaa agcggttatt tctattcctg
     9301 ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta
     9361 aaaaaggtga ggtgctattt cgactggacc cgacgcgtta tcaggtgcgg gtggatcggc
     9421 tgatggcgga tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg
     9481 agatggcggc gaatactcag caggcaaagg ccacgcggga taaattcgct aaagagtatc
     9541 agcgttacgc acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg
     9601 tggcgcggca aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa
     9661 aacagatcca gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc
     9721 tgaaagcgca gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga
     9781 gcgatggtta tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc
     9841 tacgtccggt gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc
     9901 agaactcctt gctgcgactg gcccctggcg acgatgcgga agtggtgttt aatgctctgc
     9961 caggtaaggt attcagcggc aagctggcag ccattagtcc agctgttccc ggcggagctt
    10021 atcagtcgac cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg
    10081 cgaccattga actggatgag cacactgatt tgagcgcttt accagacggt atttacgccc
    10141 aggtggcggt ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctattac
    10201 gcatgaccag ctgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt
    10261 gttcaggccg cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat
    10321 ccagcacata ctggacccgc cagggcaggt attcccgttt acggaagata gccaccagat
    10381 ccagccacca ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcagatcgc
    10441 tctgcacact tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg
    10501 ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac
    10561 tcccggcgac gttgaagcgc cagatattcg cgtcgtcgat catcatcgag tcgatcaaaa
    10621 tacaggagtg atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct
    10681 ggctggcata agcggttacg gcatattgtg taataaaact ggcaaccagc gattcatctt
    10741 ttggtggagc gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa
    10801 aggcattacg ctcaa
//
LOCUS       DADWTH010000122        10698 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.124, whole genome shotgun sequence.
ACCESSION   DADWTH010000122 DADWTH010000000
VERSION     DADWTH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.124"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            126..1823
                     /gene="maeA"
                     /locus_tag="JNY02_003447"
     CDS             126..1823
                     /gene="maeA"
                     /locus_tag="JNY02_003447"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBB9434861.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="JNY02_003448"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="JNY02_003448"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBB9434862.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="JNY02_003449"
     CDS             3113..3397
                     /locus_tag="JNY02_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBB9434863.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="JNY02_003450"
     CDS             complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="JNY02_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBB9434864.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4093..4977)
                     /gene="fdnH"
                     /locus_tag="JNY02_003451"
     CDS             complement(4093..4977)
                     /gene="fdnH"
                     /locus_tag="JNY02_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBB9434865.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="JNY02_003452"
     CDS             complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="JNY02_003452"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7450..7452),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBB9434866.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8269..9150
                     /gene="yddG"
                     /locus_tag="JNY02_003453"
     CDS             8269..9150
                     /gene="yddG"
                     /locus_tag="JNY02_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBB9434867.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9409..10509
                     /gene="ompC"
                     /locus_tag="JNY02_003454"
     CDS             9409..10509
                     /gene="ompC"
                     /locus_tag="JNY02_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBB9434868.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2622 a         2743 c         2654 g         2679 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcac cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcacctt ataaagcgcc
     3421 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3481 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3541 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3601 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3661 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3721 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3781 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     3841 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     3901 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     3961 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4021 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4081 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4141 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4201 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4261 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4321 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4381 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4441 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4501 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4561 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4621 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4681 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4741 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaacaccga cgcagtgccc
     4801 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     4861 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     4921 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     4981 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5041 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5101 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5161 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5221 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5281 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5341 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5401 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5461 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5521 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5581 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5641 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5701 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5761 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     5821 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     5881 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     5941 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6001 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6061 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6121 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6181 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6241 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6301 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6361 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6421 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6481 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6541 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6601 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6661 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6721 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6781 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     6841 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     6901 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     6961 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7021 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7081 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7141 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7201 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7261 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7321 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7381 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7441 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7501 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7561 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7621 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7681 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     7741 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7801 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     7861 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     7921 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     7981 tgttccagcc atacctcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8041 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8101 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8161 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8221 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8281 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8341 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8401 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8461 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8521 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8581 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8641 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8701 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8761 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     8821 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     8881 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     8941 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9001 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9061 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9121 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9181 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9241 atctcatttc gacttcgatc acatatcgat aaataatatt atttcatcta tttttctcat
     9301 tgtttttaat caccattcta ttttaaaaac agtatgtcat ttataaatga cgccgtttaa
     9361 aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
     9421 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
     9481 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
     9541 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
     9601 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
     9661 aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa
     9721 attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta cgacattggt
     9781 tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa agatgtcttc
     9841 atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt tggcttaatt
     9901 gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga caacagtcat
     9961 ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat
    10021 gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac aaatgcgcag
    10081 gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact atgggccaca
    10141 ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga aacattaaac
    10201 atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga agtggtggcg
    10261 caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc gaaaggcaga
    10321 gatctgggcc gctacgccga ccaggacatg attgagtata tcgacgttgg tgcgacgtat
    10381 ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat tgatgaaagc
    10441 gactttaccc gtgccgtaga tattcgcacc gataacatcg tcgctacggg cattacctat
    10501 cagttctaac gtcctctcct gaccagccct tctctgggct ggtttttgat ttaagtgctg
    10561 ctgtatgact ccttaagtaa aaatgagcaa tttttgatat ttctggtcat tcacctcaat
    10621 taatattatt ccatatcctc cggggacatc ttactataat atttattgat gagtgattat
    10681 gaccaatatt aatacagc
//
LOCUS       DADWTH010000123        10293 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.125, whole genome shotgun sequence.
ACCESSION   DADWTH010000123 DADWTH010000000
VERSION     DADWTH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.125"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..401
                     /locus_tag="JNY02_003455"
                     /pseudo
     CDS             1..401
                     /locus_tag="JNY02_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="JNY02_003456"
     CDS             484..741
                     /locus_tag="JNY02_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434869.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JNY02_003457"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JNY02_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBB9434870.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JNY02_003458"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JNY02_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBB9434871.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JNY02_003459"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JNY02_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBB9434872.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JNY02_003460"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JNY02_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBB9434873.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JNY02_003461"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JNY02_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBB9434874.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JNY02_003462"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JNY02_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBB9434875.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="JNY02_003463"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="JNY02_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBB9434876.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="JNY02_003464"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="JNY02_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="JNY02_003465"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="JNY02_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="JNY02_003466"
     CDS             9762..10019
                     /locus_tag="JNY02_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBB9434877.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2400 a         2734 c         2822 g         2337 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agc
//
LOCUS       DADWTH010000124        10205 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.126, whole genome shotgun sequence.
ACCESSION   DADWTH010000124 DADWTH010000000
VERSION     DADWTH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.126"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            31..834
                     /gene="hpaI"
                     /locus_tag="JNY02_003467"
     CDS             31..834
                     /gene="hpaI"
                     /locus_tag="JNY02_003467"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBB9434878.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALTARFGNSSSISMAKQNNNSVY"
     gene            856..2211
                     /gene="hpaX"
                     /locus_tag="JNY02_003468"
     CDS             856..2211
                     /gene="hpaX"
                     /locus_tag="JNY02_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBB9434879.1"
                     /translation="MTTSSLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2221..3111
                     /gene="hpaA"
                     /locus_tag="JNY02_003469"
     CDS             2221..3111
                     /gene="hpaA"
                     /locus_tag="JNY02_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBB9434880.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3138..4075
                     /locus_tag="JNY02_003470"
                     /pseudo
     CDS             3138..4075
                     /locus_tag="JNY02_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4302..5860
                     /gene="hpaB"
                     /locus_tag="JNY02_003471"
                     /pseudo
     CDS             4302..5860
                     /gene="hpaB"
                     /locus_tag="JNY02_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5878..6390
                     /locus_tag="JNY02_003472"
     CDS             5878..6390
                     /locus_tag="JNY02_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBB9434881.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGESRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6783..8933
                     /gene="btsT"
                     /locus_tag="JNY02_003473"
     CDS             6783..8933
                     /gene="btsT"
                     /locus_tag="JNY02_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBB9434882.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8983..9186
                     /locus_tag="JNY02_003474"
     CDS             8983..9186
                     /locus_tag="JNY02_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBB9434883.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9197..10153
                     /gene="yjiA"
                     /locus_tag="JNY02_003475"
     CDS             9197..10153
                     /gene="yjiA"
                     /locus_tag="JNY02_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBB9434884.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2428 a         2656 c         2643 g         2478 t
ORIGIN      
        1 ttagctgccg ctttgtttaa ggagagaacg atggaaaaca gttttaaagc ggcgctgaaa
       61 gcaggccgtc cgcagattgg attatggctg gggctgagca gcagctacag cgcggagtta
      121 ctggccggac caggattcga ctggttattg atcgacggtg agcacgcgcc gaataacgtg
      181 caaaccgtgc tcacccaact acaggcgatt gcgccctacc ccagccagcc agtggtacgc
      241 ccatcgtgga acgatccggt gcaaatcaaa caactgctgg acgtaggcac acaaacctta
      301 ctggtgccga tggtacaaaa cgccgacgaa gcccgtaaag cggtacgcgc cacccgttat
      361 ccccccgccg gtattcgcgg tgtgggcagt gcgctggctc gcgcctcgcg ctggaatcgc
      421 attcctgatt acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg
      481 cgtgaggcaa tgaagaactt accgcagatt ctggacgtgg aaggggtcga cggcgtgttt
      541 atcggcccgg cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa
      601 gtacaggccg ccattgagca ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg
      661 atcctgatcg ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc
      721 gccgtcggcg ttgacaccac cctgctcgcc cgcgccgccg aagcgctgac agcacggttt
      781 ggtaatagtt cttctatttc gatggcgaag caaaacaaca actctgtcta ctaaacgtac
      841 gatgtggagc gcatcatgac gacctctagc ctgcaatata aagataataa aactgttgaa
      901 attgaacatc gtgtcattaa taaattattt cgtcgcctaa tcatttttct ttttattctg
      961 tttgtttttt cgtttcttga tcgcatcaat ataggatttg ccggactgac aatgggcaag
     1021 gatcttggcc tgacgtccac aatgtttggc ctcgccgcga cactctttta tgtcacgtac
     1081 gtcatgtgcg ggatccccag taatatgatg ttagcgaaaa ttggcgctcg tcgatggatc
     1141 gctgggatca tggtggtctg gggaattgct tccacctgca caatgtttgc aacaagcccc
     1201 gaaacactct atctactgcg tatgctggtt ggtattgctg aggcgggttt tttacctggt
     1261 attttggttt atctgacctg gtggttcccg gcatatcatc gggccagagc caatgcctta
     1321 tttatgatag cgatgccagt cactatgatg ctgggattga tcctttcagg ctacattctg
     1381 gccttggatg gactgtggaa tctaaaaggc tggcaatggc tctttttgct ggaaggtctg
     1441 ccatcggtca ttcttggtgt cgtcacctgg ttttatctca acgacacacc agaccaggcg
     1501 acctggcttg atgaaaacga aaaacaggct ttaaaaacga tgattctccg tgaacaagag
     1561 agtgcatttg cccatgcatc tgttccacgc tcaaccttac gtgaagtcct gaccccagcc
     1621 atcatgctat atacacttgc ttatttttgc ctgactaata ctctgagtgc gattaatatc
     1681 tggacgccgc aaatcctaca gagtttcaat actggcagta gcaacatcat gattggtttg
     1741 ctggcggcca tcccccaatt ctgcacaatt tttggcatga tctggtggag tcgtcgttcc
     1801 gaccgtctta aagaacgcaa aaagcatacg attcttccct acctgtttgc cgctgccgga
     1861 tggttactcg cttctgcgac agatcacagc ctcattcaac tgagcggcat cattatggcc
     1921 tcaatgggat catttaccgc gatgactatc ttttggacaa cacaggatca agttataagc
     1981 ttgcaatcgc gtgccgttgc gatggctgtc atcaatgctg tcggcaatgt aggttcagca
     2041 gtcagtccat tacttatcgg tattttgaaa gatgcaaccg gcagttttag ctcaggtttg
     2101 tggtttgtag caggattgct ggtactgggc gcgttagtca tgacccgaat ccctatgggg
     2161 agtcgggaac actctctttc atctgaactt atcgcaccaa aaccacaata aggagtcatg
     2221 atgtccagtg aagctatctt tgaacatggt tatgaaaata ttgatttggt ccgggaatat
     2281 gatgcccgct acgctggtga agatgtgcat tatgaaacat ttacccggtt agcgaatttc
     2341 tttggacgcg atatgcgccc tcactggcac gatagatatt ttcaactcca ttatctggtc
     2401 actggacgca ttactttaca actggatgag cacttctatt ctgtgcgcgc accgcttttt
     2461 gtactaacac caccatcggt gccacatact ttctttactg atcctgatag cgatgggcat
     2521 gttctgaccg tacgtcagga gttaatttgg cccctggtag aaaagctctg gcctggtagt
     2581 ggagatgccg caatgctaca aggtatctgt ctgtcaccgg agggaatgga acatacactg
     2641 gcggcattaa atcattattg gccgataata tccctggagt ttcaacaaaa taagccagga
     2701 cgggaaattc tattgcaatc actggcgcaa agtattttta caatcctact gcgtgaagca
     2761 ccaccaaatg atatttcaac atgcagcgtg cgcggcgaga tgtgtttatt ccagaagttt
     2821 aatcggctga ttgacgaaaa ttatcgccaa catttaatgg tccctgaata tgcaacaatg
     2881 ctcggtatga gtgaatcacg gcttaccgag ctgtgccgac gttttgccaa tcaatcgccg
     2941 aagagattga ttttcgaacg agtgagccgg gaagccagac ggcttctgct ctatagcgag
     3001 caaagcatca accagatagc ccttgatctt ggctacaaag accccgccta tttcgcccgt
     3061 tttttcaacc gaatgatggg ttgctcacca acacaattcc gtggtcgctg accatttatc
     3121 aacctgagag actcgacatg aaaatatctt tacgctcact atttgtggcg tgcctgattg
     3181 cgccagtggc attcacctac gcctccgaac cgcaataaat gaaaattgta ctacttggaa
     3241 cgaaaggtgg cccttcttta ttaaaagctg accgtttacc gatggcaacg gcacttatta
     3301 ttggcaataa aacctggtta attgatgctg gttacggggc cagcttacaa ctggtaaaaa
     3361 ataatattcc actgcgccag attgataata ttttactcac ccatttacac agtgatcata
     3421 ttctggacta tccatcagtc ttaatgaatg catgggctag cggtctgcag gacaaaacta
     3481 ttcatgtatt cggtccgcca ggcacccgag ccatgaccgc tgccagttgg caggtcttcg
     3541 atcgggacat cacactgcgt atggaagaag aaggaaaacc cgatcctcgc aaactcgtta
     3601 cagctcaaga tgttggtgaa ggcgtagttt ataaagatga taccgtgacc gtgactgcat
     3661 taaaggtccc tcattctcct ttccctgacg gtgaggcttt tgcttatcgc ttcgacacac
     3721 aaggcaagcg aattgtcttc tcaggagata cctcgtactt tcccccactg gcaactttct
     3781 cagaaggcgc tgatatactt gtgcatgagg ccgtacatga gccctccgtc gctaagctgg
     3841 cagacagtat tggcaatggt aaaacactgg caaaagccat tgcttctcac catacgacta
     3901 ttgaggatgt aggcaaaatt gccaatgaag caaaagtgaa aaaactcatc cttagccacc
     3961 tggtaccaat caccgttccg gatgaaacat ggaagcaaga agcacagaaa actattcagg
     4021 ccctatcgtc gttggtcatg aaaatatgac gattaccatt ccattcgccc cataaaaaat
     4081 tccgtccccg ttagcccttg ttagcgggga atattatgat ttgccttact gtaagttgat
     4141 cacgatcgca gtttactatt taactccacc ttgctgtcga aaagtaccaa cgttcgcctg
     4201 aaccgtctct tcccgacaac cgcgttcaac gcttcaatta aataacaaaa acaacacaca
     4261 aaattaacaa tcgtattccg attaatactg tagaggtcga catgaaacca gaagatttcc
     4321 gcgccagtac ccaacgtcct ttcaccgggg aagagtatct gaaaagcctg caggatggtc
     4381 gcgagatcta tatctatggc gagcgagtga aagacgtcac cactcatccg gcatttcgta
     4441 atgcggcagc gtctgttgcc caactgtacg acgcgctgca caaaccggag atgcaggact
     4501 ctctgtgttg gaacaccgac accggcagcg gcggctatac ccataaattc ttccgcgtgg
     4561 cgaaaagtgc cgacgacctg cgccagcaac gcgacgccat cgctgaatgg tcacgcctga
     4621 gctatggctg gatgggccgt accccagact acaaagccgc tttcggttgc gcactgggcg
     4681 cgaatccggg cttttacggt cagttcgagc agaacgcccg taactggtat acccgtattc
     4741 aggaaactgg cctctacttt aaccacgcga ttgttaaccc accgatcgtc atttgccgac
     4801 cgataaagtg aaagacgttt acatcaagct ggaaaaagag actgacgccg ggattatcgt
     4861 cagcggtgcg aaagtggttg ccaccaactc ggcgctgact cactacaaca tgattggctt
     4921 cggctcggca caagtgatgg gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat
     4981 ggatgccgat ggcgtgaaat taatctcccg cgcctcttat gagatggtcg cgggtgctac
     5041 cggctcgcca tacgactacc cgctctccag ccgcttcgat gagaacgatg cgattctggt
     5101 gatggataac gtactgatcc catgggaaaa cgtgctgatc taccgcgatt ttgatcgctg
     5161 ccgtcgctgg acgatggaag gcggtttcgc ccgtatgtat ccgctgcaag cctgtgtgcg
     5221 cctggcagtg aaactcgact tcattacggc actgctgaaa aaatcactcg aatgtaccgg
     5281 caccctggag ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt ggcgcaacac
     5341 cttctgggca ttgagtgact cgatgtgttc agaagcgacg ccgtgggtca acggggctta
     5401 tttaccggat catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa
     5461 gatcaaaaac attatagaac gcaacgttac cagtggcctg atctacctcc cttccagtgc
     5521 ccgtgacctg aacaatccgc agatcgacca gtatctggcg aagtatgtgc gcggttcgaa
     5581 cggtatggat cacgtccagc gcatcaagat cctcaaactg atgtgggacg ccattggcag
     5641 cgaatttggt ggtcgtcacg agctgtatga aatcaactac tccggtagcc aggatgagat
     5701 tcgcctgcaa tgtctgcgcc aggcacaaag ctccggcaat atggacaaga tgatggcgat
     5761 ggttgatcgc tgcctgtcgg aatacgacca gaacggctgg actgtgccgc acctgcacaa
     5821 caacgacgat atcaacatgc tggataagct gctgaaataa cgcagcagga ggttaagatg
     5881 caattagatg aacaacgcct gcgctttcat gacgcgatgg tcagcctgtc ggcagcggta
     5941 aatattatca ccaccgaggg cgacgccgga caatgcggga ttacggcaac ggctgtctgc
     6001 tcggtcacgg atacaccacc gtcgctgatg gtgtgcatta acgccaacag tgcgatgaac
     6061 ccggtttttc agggcaacgg caagttgtgc gtcaacgtcc tcaaccatga gcaggaactg
     6121 atggcgcgcc acttcgcggg catgacaggc atggcgatgg aagagcgttt tagcctctca
     6181 tgctggcaaa aaggtccgct ggcgcagccg gtgctaaaag gttcgctggc cagtcttgaa
     6241 ggagagagcc gcgatgtgca ggcgattggc acacatctgg tgtatctggt ggagattaaa
     6301 aacatcatcc tcagtgcaga aggtcacgga cttatttact ttaaacgccg tttccatccg
     6361 gtgatgctgg aaatggaagc tgcgatttaa gctccagccc cttcacgcat tcgtcgaagg
     6421 ggcattttta ttgtgatctc aatcacatat ctacccgatg aataattctt gcccaaccgc
     6481 tcaccgccat tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt
     6541 tacgcgcacg tatctctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac
     6601 gcaacgcaaa cctagaacgg cttcggccta tgattaacaa attactctat ggattttggt
     6661 ttgcaggaag gcagcaagtg agtgaatccc cgggagctta caaaagtaag tgtcagaggt
     6721 gaacgaacgc agctgccgca cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta
     6781 ctatggatac gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg
     6841 cattctgcct cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg
     6901 tggtcgcctc tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc
     6961 agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc
     7021 tgaactacgt tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg
     7081 gtgctggtcc gctggtggga ccggttctcg ccgcgcagat gggctacctg cctggcacgc
     7141 tgtggctgct ggcgggggta gtgctggccg gtgcggtaca ggactttatg gtgctgttta
     7201 tctcctctcg ccgtaacggc gcatctctcg gtgagatgat caaagaagag atggggccag
     7261 taccggggac tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgttc
     7321 tggcgctgat tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc accgtttgct
     7381 caaccgtacc gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg
     7441 tgggtgaagt ctctgttatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg
     7501 tgattgctca cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct
     7561 tcgcgctgat tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac
     7621 cgcgcgacta tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta
     7681 tcgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg
     7741 gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg
     7801 tatctggctt ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa
     7861 ccgacgcgcg tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg
     7921 cgctggttgc agcgtccatc atcgaaccgg gtctttactt cgcgatgaac accccgcctg
     7981 ctggccttgg catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca
     8041 tcatggcgca gctgaaagac gttaccgcac acgcggcagc gaccgtcagc tcctggggct
     8101 tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc
     8161 tgaaccgtgc aggtggtgcg ccgacgctgg cggtaggtat cgctcacgtg ttccacaaag
     8221 tgctgccgat ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt
     8281 tcatcctgac cgcgctggat gcgggtaccc gttctggccg ctttatgctg caagacctgc
     8341 tgggtaactt catcccgttc ctgaaaaaaa ccgactctct ggttgccggt atcatcggta
     8401 ctgcgggctg tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg
     8461 gcggcgttaa gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag
     8521 cgctggtact gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa
     8581 ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt
     8641 tcagcaccaa cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga
     8701 ttgctaacgg tactgacctg acggcgcagc agattgccaa catgaaccac atcgttgtga
     8761 acaactacac caacgcaggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct
     8821 tctacggttt caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa
     8881 caccgtacgt tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtgtt
     8941 tagccccgct tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga
     9001 caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggtattcc agactacgac
     9061 aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa
     9121 ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt
     9181 tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc
     9241 gggcaaaact accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt
     9301 gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac
     9361 gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggagga
     9421 cgcactactc gacctgctgg ataatctcga caagggcaat attcagttcg atcgtctggt
     9481 gattgaatgc accggcatgg ccgatcccgg cccgattatt cagacctttt tctcccatga
     9541 gattttatgc cagcgctacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc
     9601 cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gtgggctacg ccgaccgtat
     9661 tctgctgact aaaaccgacg tcgcaggcga agcagagaaa ctgcgtgaac gcctggcgcg
     9721 catcaacgcc cgcgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct
     9781 gttcaacacc aacggtttta tgctggaaga gaacgtcgtc agcaccaaac cgcgtttcca
     9841 ctttatcgcg gataaacaaa acgatatttc gtcaattgtg gtggaactgg attacccggt
     9901 agatatcagc gaagtttccc gcgtgatgga aaacctgctg ctggaatcgg cggataaact
     9961 gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg
    10021 cgtccagcgc ctctacagcg ccgactggga caggccgtgg ggcgatgaaa aaccgcatag
    10081 cacaatggtg tttatcggga ttcagttgcc ggaggaagag attcgggcgg cgtttgcggg
    10141 attgaggaag taagttcggg aaagatcaac tttttgagag ttccaccgca acattattac
    10201 gagtg
//
LOCUS       DADWTH010000125        10073 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.127, whole genome shotgun sequence.
ACCESSION   DADWTH010000125 DADWTH010000000
VERSION     DADWTH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.127"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(296..1156)
                     /locus_tag="JNY02_003476"
     CDS             complement(296..1156)
                     /locus_tag="JNY02_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBB9434885.1"
                     /translation="MALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFD
                     LNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARA
                     AREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQPSTITMISDW
                     LAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADILSPVKETGTLLGVVS
                     SQAAELCGLKAGTPVVVGGGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQ
                     LPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JNY02_003477"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JNY02_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBB9434886.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="JNY02_003478"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="JNY02_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBB9434887.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAGTERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAEDNATPERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="JNY02_003479"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="JNY02_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBB9434888.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="JNY02_003480"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="JNY02_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBB9434889.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWFWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="JNY02_003481"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="JNY02_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..7965
                     /gene="lsrF"
                     /locus_tag="JNY02_003482"
     CDS             7090..7965
                     /gene="lsrF"
                     /locus_tag="JNY02_003482"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBB9434890.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7989..8279
                     /gene="lsrG"
                     /locus_tag="JNY02_003483"
     CDS             7989..8279
                     /gene="lsrG"
                     /locus_tag="JNY02_003483"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBB9434891.1"
                     /translation="MHVTLVEINVHEDKVEEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8318..9076
                     /gene="tam"
                     /locus_tag="JNY02_003484"
     CDS             8318..9076
                     /gene="tam"
                     /locus_tag="JNY02_003484"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBB9434892.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9080..9994)
                     /locus_tag="JNY02_003485"
     CDS             complement(9080..9994)
                     /locus_tag="JNY02_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBB9434893.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTYISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         2431 a         2482 c         2653 g         2507 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatggctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt caagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgttctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 ggaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcgg aagataatgc caccccggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tagaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgc ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atccccaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggt tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgctggattg gggaatgcag
     7201 tcacgcttat cgtggatatt taatccgaaa acgggtaaaa ctgtgatgct ggcctttgac
     7261 catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa tatcgccccg
     7321 ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag cgtagttccc
     7381 ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg cgaactctat tctggcggaa
     7441 ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg
     7501 gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa aaatattatt
     7561 cagctggtcg atgccggaat gaaagtggga atgccgacca tggccgtgac tggcgtgggc
     7621 aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg
     7681 ggggcgcaaa ttatcaaaac ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga
     7741 tgtccggtac ccattgttat tgctggcggt aaaaaattac cggagcgcga ggcgctggaa
     7801 atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg taatattttc
     7861 cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggttca ccataacgaa
     7921 acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg gatttaagga
     7981 gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagacaag gttgaagagt
     8041 ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggt aatttgcgct
     8101 tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatttacgaa gcctataaag
     8161 atgaagacac agtggcattc cataaaacca cgcctcacta caaaacctgt gtcgcgaaac
     8221 tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     8281 gctacaattg gggtaacgcg ctgacgggag taaaaaaatg tctgactgga acccctcttt
     8341 atatctgcac ttttccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc
     8401 gctggagaat gtcgaatatg ttgccgatct tggctgtggc ccaggtaaca gcaccgccct
     8461 gctgcatcaa cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat
     8521 tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg gaagcagaca tccgcaactg
     8581 gcaaccggaa caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga
     8641 ccactacgaa ttgtttcctc acttggtttc tttacttaat ccgcagggcg tgctggcagt
     8701 acagatgcca gataactggc tggagccgac ccatgtgctc atgcgcgaag ttgcttggga
     8761 acaaaactac ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt actacgatat
     8821 tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc acttactatc accagatgcc
     8881 gtcgcaccag gcgattatcg attgggtgac cgccactgga ttacgtccgt ggttacagga
     8941 tctgaccgag agcgaacagc agctttttct tacgcgctac catcagatgc tggaagagca
     9001 gtatccactg caagagaacg gacagatact gctggcattt ccgcgtctgt ttattgttgc
     9061 ccgccgtacg gagtaattat cacgtcagct ggtaatgacg atcgggaaga atttttgctg
     9121 gaatttctgc ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat
     9181 ccagcggtaa atcattgttt tcagtaccga acggatcttc cagttcttcc gccagacaat
     9241 ccagcgaaat ataagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt
     9301 ccacgaccag cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca
     9361 gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg
     9421 aaatatcatt aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgtccattgc
     9481 ggcgctgaac cgccaaccat tctcccatta ttaacaagat acggttagct ggagagttcg
     9541 aagccagtac acgctgaaga tctccagtct tgagataatg agccagcact tccgcctgtg
     9601 gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc ctggcaaact
     9661 cccttacact tgccgaatcc ggcaatgtcg tttttacctc tcgcagtaac gaccgtgagg
     9721 caatcatcaa ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat
     9781 tacgaaaacc aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga
     9841 atttgatgcc cagatgcgtg taccagggca gcatgaatat aacagcaata gaaaagagaa
     9901 aattgagtag taagcgcgag gatatcttgg ataatactga gccgtgccag acaaaaatac
     9961 ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg
    10021 ccctattaaa cgtgattacg atcacattct caagacgcta cttacaaatt acc
//
LOCUS       DADWTH010000126         9957 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.128, whole genome shotgun sequence.
ACCESSION   DADWTH010000126 DADWTH010000000
VERSION     DADWTH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.128"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..272)
                     /locus_tag="JNY02_003486"
     CDS             complement(<1..272)
                     /locus_tag="JNY02_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBB9434894.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="JNY02_003487"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="JNY02_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="JNY02_003488"
     CDS             complement(1132..1338)
                     /locus_tag="JNY02_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBB9434895.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="JNY02_003489"
     CDS             complement(1445..2008)
                     /locus_tag="JNY02_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBB9434896.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="JNY02_003490"
     CDS             complement(3383..5218)
                     /locus_tag="JNY02_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434897.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="JNY02_003491"
     CDS             5319..5606
                     /locus_tag="JNY02_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBB9434898.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="JNY02_003492"
     CDS             5578..7107
                     /locus_tag="JNY02_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBB9434899.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="JNY02_003493"
     CDS             complement(7277..8494)
                     /locus_tag="JNY02_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB9434900.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="JNY02_003494"
                     /pseudo
     CDS             complement(8861..9847)
                     /locus_tag="JNY02_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9844..>9957)
                     /locus_tag="JNY02_003495"
     CDS             complement(9844..>9957)
                     /locus_tag="JNY02_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBB9434901.1"
                     /translation="EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
BASE COUNT         3139 a         2263 c         1843 g         2712 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg cggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagataga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg taccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcaacg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctact
     9601 ttttcttaaa ccacaaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttc
//
LOCUS       DADWTH010000127         9955 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.129, whole genome shotgun sequence.
ACCESSION   DADWTH010000127 DADWTH010000000
VERSION     DADWTH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.129"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNY02_003496"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNY02_003496"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBB9434902.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNY02_003497"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNY02_003497"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBB9434903.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="JNY02_003498"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="JNY02_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="JNY02_003499"
     CDS             3456..5216
                     /locus_tag="JNY02_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBB9434904.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="JNY02_003500"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="JNY02_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBB9434905.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="JNY02_003501"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="JNY02_003501"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBB9434906.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="JNY02_003502"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="JNY02_003502"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBB9434907.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIYT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2551 a         2495 c         2378 g         2531 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccaag
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttgggt aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caacctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatggggcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcatttat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcattctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DADWTH010000128         9725 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.130, whole genome shotgun sequence.
ACCESSION   DADWTH010000128 DADWTH010000000
VERSION     DADWTH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.130"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            429..1547
                     /locus_tag="JNY02_003503"
     CDS             429..1547
                     /locus_tag="JNY02_003503"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBB9434908.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="JNY02_003504"
     CDS             1613..1861
                     /locus_tag="JNY02_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBB9434909.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="JNY02_003505"
     CDS             1926..2294
                     /locus_tag="JNY02_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBB9434910.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="JNY02_003506"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="JNY02_003506"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBB9434911.1"
                     /translation="MNIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="JNY02_003507"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="JNY02_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBB9434912.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTKAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="JNY02_003508"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="JNY02_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBB9434913.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGDAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGRNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9076)
                     /gene="cusA"
                     /locus_tag="JNY02_003509"
                     /pseudo
     CDS             complement(5941..9076)
                     /gene="cusA"
                     /locus_tag="JNY02_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9088..9629)
                     /locus_tag="JNY02_003510"
                     /pseudo
     CDS             complement(9088..9629)
                     /locus_tag="JNY02_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2387 a         2694 c         2351 g         2293 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatt tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcattt tattgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt gaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg aatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 ccgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccaaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgtcat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccaccag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggattcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccgg
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 tgaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgtcac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctcg gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac agcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt taatgccccg gcgcatctcc gggccaatct
     8281 ggtccttcgc aacatcgcgc agataaacgg gcacgccatt ttcactggct tttaaaacga
     8341 tgtgattaaa gtcgtcgagc gtttgtagat agccgctggc gcgcaccata tattctgctt
     8401 ccgccagttc gattgacgaa ccgcccgctt cctggtttga agcatccagc gcgcttttta
     8461 cttcggcgag actgatgcca tactgcgcca ggcgctgagg atcgataacc acctgatact
     8521 ctttcaccac accgcctacc gacgccactt ccgcaacgtc aggaatggtt ttcagctcat
     8581 atttgagaaa ccagtcctgc aatgagcgta aatcggcaag atcgtgctta ccgctgcgat
     8641 ccaccagtgc atattcatag atccagccaa cacccgtggc atctggtccc agctcggcgc
     8701 tgactcccgc aggcagctta ccctgtacct ggttgaggta ctccagcacc cgcgaacgcg
     8761 cccagtacgg atcggtgcca tcttcgaaaa tgacatacac ataagagtcg ccaaactgcg
     8821 agaaaccacg cacagtcttc gcgccaggca ccgacaacat ggtggtggtt agcggataag
     8881 tcacctgatt ttcaacgatt tgcggtgcct ggccgggata gctggtttta ataatcacct
     8941 gcacatcgga gagatccggc aacgcatcca ctggcgtatt aatgatggtc caggtgcccc
     9001 agatgctcag aaacaacgcg cccatcagca ccaaaaaacg gttcgccacc gagcgacgaa
     9061 taatccattc aatcattggt tattccctca atgcgcatgg gtagcacttt cagagcgcat
     9121 ccgctccagt gcgccagaaa tattggcttc ggaatcaatc aggaacaggc cgccggaaac
     9181 caccttttca ccttccgcca gaccagagcg taatgcggtg acgccttgcg atgcctggaa
     9241 aacagcaacg cgtttcggta caaagcgccc gtcggcatca acggtaatca cccgctgttc
     9301 gttgccggta tcaatcagcg cctcgtcggc gttgtcgact tccagacgca gctgcagcgt
     9361 gcgggtcgcg gcatccacgc caggtagcag cgtccatttg cggatggtga gtgttttatc
     9421 cggtcgcgcc ggaacggtga gggtaaactg cgaggcatct ttcaccagcc aggcgatgga
     9481 ctccgggatc gcagcagtga cccacaccgg gtccataccc tgaattcgcg attacgcgca
     9541 atacccctga gaaccgccat gaacaagggt ttcatgattt gtgattttag tttagtacgc
     9601 aatttggtac acaacactat tttcacttca gcgggtagtc atcaaactca ccataacgag
     9661 catcgttgat gatgtatgtg atcaccacaa atatcacatc tggactagtt ccgtcttcat
     9721 gcgca
//
LOCUS       DADWTH010000129         9525 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.131, whole genome shotgun sequence.
ACCESSION   DADWTH010000129 DADWTH010000000
VERSION     DADWTH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.131"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(435..641)
                     /locus_tag="JNY02_003511"
     CDS             complement(435..641)
                     /locus_tag="JNY02_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBB9434914.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JNY02_003512"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JNY02_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBB9434915.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="JNY02_003513"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="JNY02_003513"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBB9434916.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="JNY02_003514"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="JNY02_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="JNY02_003515"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="JNY02_003515"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBB9434917.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..6343
                     /locus_tag="JNY02_003516"
                     /pseudo
     CDS             5360..6343
                     /locus_tag="JNY02_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            6343..8042
                     /locus_tag="JNY02_003517"
                     /pseudo
     CDS             6343..8042
                     /locus_tag="JNY02_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            8039..8716
                     /gene="kdpE"
                     /locus_tag="JNY02_003518"
     CDS             8039..8716
                     /gene="kdpE"
                     /locus_tag="JNY02_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBB9434918.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8991..9095
                     /locus_tag="JNY02_003519"
     CDS             8991..9095
                     /locus_tag="JNY02_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBB9434919.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9406..>9525
                     /locus_tag="JNY02_003520"
     CDS             9406..>9525
                     /locus_tag="JNY02_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBB9434920.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2116 a         2396 c         2640 g         2373 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa acatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctccccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttaccac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcta ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttaatcgca ctgtttttta ttcaacaagg
     1621 tgcgctgcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatactt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgaccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttaccg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgccgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctccgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gcgaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gctgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctatt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg actagccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctggcg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaac cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcagaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggtggcgct attttatgga cgctggcctt cggtggttgc caccgtcatt
     6721 aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat
     6781 gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt
     6841 actgctggcg tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac
     6901 ttatatgaaa tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc
     6961 agcgaacaat ttattgcctc tacgtttcat gcccgcagtc aggtgttgtt gcccgatgac
     7021 aacggtaaat tgcagccgtt aacacatccg caaggaatga cgccatggga cgatgccatc
     7081 gcgcagtgga gttttgataa aggcctgcct gcgggcgcgg gcaccgacac gttacccggt
     7141 gtaccgtacc agattttgcc gctaaaaagc ggcgagaaaa cctacggctg gtggtggtgg
     7201 agccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg gagactttta
     7261 cgctgttagt cgccaatgcc cttgagcggc tgacgctaac cgccagcgaa gaacaggcgc
     7321 ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcagcgctt tcgcatgatt
     7381 tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa
     7441 gcgaaggatc accccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta
     7501 cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga
     7561 agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ctggaaccgg
     7621 gtttatcgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg
     7681 ggccactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg
     7741 cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct
     7801 gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc
     7861 gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg gttggcaatt tgtcgggcga
     7921 ttgtggatgt acacggcggc accattaccg cgttcaaccg accggaaggt ggtgcctgtt
     7981 ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt
     8041 gacaaacgtt ctgattgttg aagatgaaca ggctattcgt agctttctgc gcacggcgct
     8101 ggagggcgac gggatgcgcg tctttgaggc cgaaactctg caacgcggct tgctggaagc
     8161 ggcaacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat
     8221 tgagtttatc cgcgacctgc gccagtggag cccggtgccg gtgattgtgc tttccgcacg
     8281 cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt atctgagtaa
     8341 gccgtttggc attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc
     8401 caccgccgcg cccgatccgc tggtgaaatt ttccgatgtt accgtcgatt tagccgcccg
     8461 cgtgattcac cggggtgagg aagaggtgca tctcacacca attgagttcc gcctgctagc
     8521 ggtgctgctc aacaatgccg gaaaagtact cacccagcgc cagctcctta accaggtgtg
     8581 ggggccaaac gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca
     8641 aaaactggaa caggatccgg cccggcctcg ccacttcatt actgaaaccg gtattgggta
     8701 tcggtttatg ctttgaattg ttattcaata aattcgctta aatagtgatt aatttattat
     8761 taaacctgcg ccgaatatta ttatttggca ggtttaattt cttaacgcaa atataaaaca
     8821 cgaaattaca acatttaagt aacaaaacag catctaatat ggcgatattc ataattaatg
     8881 atttattatc tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc
     8941 atggatagaa taactctacc ttcaactgac acagcaagcg gtaaaggtaa atggaaaata
     9001 ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt gcccatcgta
     9061 atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac
     9121 gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata
     9181 tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat
     9241 gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac
     9301 tgatttattt atcagcggtg gagctttgct ttttttccgg cgtgatcgat ttctcctttg
     9361 agaaattgag gacctgctat tacctgaaat aaagagatga ataaaatgtc agaattaaaa
     9421 attgccgtta gccgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatatt
     9481 gacgaaagta attatattga cgttgccgcc attattttat cagtc
//
LOCUS       DADWTH010000130         9520 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.132, whole genome shotgun sequence.
ACCESSION   DADWTH010000130 DADWTH010000000
VERSION     DADWTH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.132"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(194..1447)
                     /locus_tag="JNY02_003521"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="JNY02_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="JNY02_003522"
     CDS             1554..2447
                     /locus_tag="JNY02_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9434921.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="JNY02_003523"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="JNY02_003523"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBB9434922.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="JNY02_003524"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="JNY02_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBB9434923.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="JNY02_003525"
     CDS             complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="JNY02_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBB9434924.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYKVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVG
                     LLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTL
                     FIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEY
                     QLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="JNY02_003526"
     CDS             complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="JNY02_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBB9434925.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6671..7525)
                     /locus_tag="JNY02_003527"
     CDS             complement(6671..7525)
                     /locus_tag="JNY02_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBB9434926.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="JNY02_003528"
     CDS             complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="JNY02_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBB9434927.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDNPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8155..9279)
                     /locus_tag="JNY02_003529"
                     /pseudo
     CDS             complement(8155..9279)
                     /locus_tag="JNY02_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         2473 a         2412 c         2460 g         2175 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 ccagcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 tcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcgaaaa ggccacgaaa ctggggtgga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gcgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagctc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actttgtaga gtaacgcgtc gctatgatta
     5221 atcagattgc taaccagcaa tgccacgacg gcggaaataa tcaccgggcc gagagaggcc
     5281 aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga
     5341 taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca ttcctggcgt
     5401 ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct
     5461 tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg
     5521 cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca
     5581 tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg
     5641 ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga
     5701 ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg acgaaacccg
     5761 gcaacggcaa agacagcgag aataccgacg actgttgcga taagcagacg gcggaacata
     5821 gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg
     5881 ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt taagagataa
     5941 aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg taatcgtcac catccggcaa
     6001 tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac agttgtgatt
     6061 gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg
     6121 ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca
     6181 attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg
     6241 aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca
     6301 tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga
     6361 ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct
     6421 gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt
     6481 ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg
     6541 cttccgcact ctctggagcg taataaaaca gcgctcccaa cacgcgcgcc gcgacagaaa
     6601 aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg
     6661 caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca taaaagagca
     6721 cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt ccggcaacgt
     6781 gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg cataacaata
     6841 ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg
     6901 atgagtgaat agacgccaga gacaaacctt gcagcacaat cacctcggca caagcgatca
     6961 gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg gctgcccgca
     7021 gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag
     7081 tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga
     7141 cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca
     7201 ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg
     7261 cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca
     7321 gtggcgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga
     7381 aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt tttggcggcg aaccagccaa
     7441 ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg
     7501 gccattcatg ccatccattt cccattttac acttcctccg gattagccag ataaccagtg
     7561 gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa
     7621 tgcgcgcgcg gcagtggcgc gacggcagcc agagtaccgt gcttctggcg cagctcttca
     7681 attggaccga actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc
     7741 tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc agcattgtac
     7801 tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca
     7861 tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta
     7921 catgcggggt tatcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg
     7981 tgccagatgc cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa
     8041 ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
     8101 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
     8161 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgctagcg gtgaagggcg
     8221 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
     8281 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
     8341 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
     8401 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
     8461 cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc ccgttaactg
     8521 cagtggatag gttttccgca ggggagcgtc ccagccatca aaacctgagg tatacgcagg
     8581 aatgggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct tcgccagtcg
     8641 ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct gtggatcttc
     8701 acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt
     8761 cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag
     8821 atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc
     8881 taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga
     8941 ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat gagagataag
     9001 gtcttcttgc tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc
     9061 cgtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc
     9121 atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa
     9181 aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat
     9241 tttcgtacca tggtcgatgg catctgtcca tgtaaacatc tggggtcctt acagctcgtc
     9301 ctgacatgcg gtctcgcctt atatgcacat ggagaacatg gtcactccag accaacacta
     9361 atcaacatta accctattca ctttcgttaa gcactttttg gtgggtccgt ttggatgcat
     9421 tgaataggtg cggactccac atggagacag aaagtgcttt accaaaggag catcctttat
     9481 gaataccaac gaagttagcg tcatcaacct tgaccagctt
//
LOCUS       DADWTH010000131         9240 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.133, whole genome shotgun sequence.
ACCESSION   DADWTH010000131 DADWTH010000000
VERSION     DADWTH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.133"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JNY02_003530"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JNY02_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBB9434928.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JNY02_003531"
     CDS             1828..3147
                     /locus_tag="JNY02_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBB9434929.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3433..3474
                     /locus_tag="JNY02_003532"
     CDS             3433..3474
                     /locus_tag="JNY02_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434930.1"
                     /translation="MVQLVDLARCVSF"
     gene            3512..4657
                     /gene="dosC"
                     /locus_tag="JNY02_003533"
     CDS             3512..4657
                     /gene="dosC"
                     /locus_tag="JNY02_003533"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBB9434931.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="JNY02_003534"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="JNY02_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="JNY02_003535"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="JNY02_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="JNY02_003536"
     CDS             5803..6699
                     /locus_tag="JNY02_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBB9434932.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="JNY02_003537"
     CDS             6696..7682
                     /locus_tag="JNY02_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB9434933.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="JNY02_003538"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="JNY02_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JNY02_003539"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JNY02_003539"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBB9434934.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2255 a         2255 c         2321 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gtgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
//
LOCUS       DADWTH010000132         9071 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.134, whole genome shotgun sequence.
ACCESSION   DADWTH010000132 DADWTH010000000
VERSION     DADWTH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.134"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..924
                     /locus_tag="JNY02_003540"
                     /pseudo
     CDS             1..924
                     /locus_tag="JNY02_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="JNY02_003541"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="JNY02_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBB9434935.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNY02_003542"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNY02_003542"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBB9434936.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="JNY02_003543"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="JNY02_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBB9434937.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="JNY02_003544"
     CDS             4447..4806
                     /locus_tag="JNY02_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBB9434938.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNY02_003545"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNY02_003545"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBB9434939.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="JNY02_003546"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="JNY02_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBB9434940.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="JNY02_003547"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="JNY02_003547"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBB9434941.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2069 a         2200 c         2444 g         2358 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactgatat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc g
//
LOCUS       DADWTH010000133         9040 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.135, whole genome shotgun sequence.
ACCESSION   DADWTH010000133 DADWTH010000000
VERSION     DADWTH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.135"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(6..326)
                     /gene="psiF"
                     /locus_tag="JNY02_003548"
     CDS             complement(6..326)
                     /gene="psiF"
                     /locus_tag="JNY02_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBB9434942.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(445..1859)
                     /gene="phoA"
                     /locus_tag="JNY02_003549"
                     /pseudo
     CDS             complement(445..1859)
                     /gene="phoA"
                     /locus_tag="JNY02_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1960..2220)
                     /gene="iraP"
                     /locus_tag="JNY02_003550"
     CDS             complement(1960..2220)
                     /gene="iraP"
                     /locus_tag="JNY02_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBB9434943.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2402..2605)
                     /locus_tag="JNY02_003551"
     CDS             complement(2402..2605)
                     /locus_tag="JNY02_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434944.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2683..3777
                     /gene="ddlA"
                     /locus_tag="JNY02_003552"
     CDS             2683..3777
                     /gene="ddlA"
                     /locus_tag="JNY02_003552"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBB9434945.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITHLIELALERHAADNALKTTM"
     gene            complement(3801..4013)
                     /locus_tag="JNY02_003553"
     CDS             complement(3801..4013)
                     /locus_tag="JNY02_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBB9434946.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4273..4581
                     /locus_tag="JNY02_003554"
     CDS             4273..4581
                     /locus_tag="JNY02_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBB9434947.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLIQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4640..5734)
                     /gene="yaiW"
                     /locus_tag="JNY02_003555"
     CDS             complement(4640..5734)
                     /gene="yaiW"
                     /locus_tag="JNY02_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBB9434948.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5747..6967)
                     /gene="sbmA"
                     /locus_tag="JNY02_003556"
     CDS             complement(5747..6967)
                     /gene="sbmA"
                     /locus_tag="JNY02_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBB9434949.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7319..8476
                     /gene="ampH"
                     /locus_tag="JNY02_003557"
     CDS             7319..8476
                     /gene="ampH"
                     /locus_tag="JNY02_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBB9434950.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8477..8980)
                     /locus_tag="JNY02_003558"
     CDS             complement(8477..8980)
                     /locus_tag="JNY02_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9434951.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2241 a         2313 c         2148 g         2338 t
ORIGIN      
        1 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
       61 agagattgtt gcgttgcttg attattacat tcgcgcatct tttgctgctg tggcgtcaaa
      121 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
      181 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
      241 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
      301 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
      361 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
      421 gaaaattcac tgccgggcgc ggttttattt cagccccaga gcggctttca tggtgtagaa
      481 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
      541 acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac
      601 catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg
      661 cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc
      721 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
      781 ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
      841 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgccag
      901 ggttggtaca ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat
      961 attgccgtgg tacgttgctt tcggccctag ccagcgcact ggcatattgc cgtcagcaaa
     1021 caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
     1081 gttcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
     1141 accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg
     1201 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
     1261 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
     1321 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
     1381 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
     1441 gccgttatag gttttgacac cggttgacca ggcggttgct gatgcagccg agtcggtgac
     1501 gtagtccggt ttgccagttt tttattcagc gcatagtgag tgtattgccc ggtaagcggt
     1561 aaggcatcga tacctttaaa aaagccgccc gcaccttcgg cataattacg tgcggcagta
     1621 atttccgaat cccccatccc atcgccaatc agcaaaataa tattttttgc aggtttatcg
     1681 ctaagagaat cacgcagagc ggcggtctga tcacccgtta agcggcgagc accgccgggt
     1741 gcagtaatat cgccctgagc agcccggttt tccagaacag gcatttctgg tgtccgggct
     1801 tttgtcacag gggtaaacag taacggtaag agtgccagtg caatagtgct ttgtttcact
     1861 ttattttctc catgtacaaa tacattaaaa aataaaaaca aagcgactat aagtctcggc
     1921 cgtgacaact ttatgacagc tattgaaaag attaactttt tactgacgag gatgcttcaa
     1981 taacttcttt acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg
     2041 ctttacctcg tacagcgccc cctctacctg atcaatcaac cgctgttggt cattttgcgc
     2101 catattgcga agcattgcag tgacgataat ctccaaagct tctacctgcg cacacagttc
     2161 tttcgactct tcttcttttt gggcaagctt aaataacaac tcagtaatga gatttttcat
     2221 gtctgtattt ccttatccaa agtatggaga agttaacatt atgtttcaca acttcatagt
     2281 gtctggaagt attattttta tgacaaagga aatactttgc gcaaatattt tttgtttgat
     2341 taccagcgaa acgtttctct tatttaaata attacccgca caaaatatag gcttttaatt
     2401 atcaaccttt attataaata aaagctatga aatatattct cttcgttaac ttataattaa
     2461 gagaaaaaaa ctcaccgcat tgccatttaa gccaggaata aattagcttc ccgttcatca
     2521 tttttgtgat cttgcgcaca tctttgagat aacgcacttt cttcaccagt acgttgcacg
     2581 ctttgtcagc agaagattgc aacaatcttt cagaaggccg cgaatccccc gttttgtcgt
     2641 ggtaatgtat gcactttgtg ttagacggga taggttttta agatggaaaa actgcgggta
     2701 ggaatcgttt ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac
     2761 attgtcgatg ccattgataa aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa
     2821 gggcaatggc acgtcagcga tgccagcaat tatctgctaa atgcagacga tcctgcccat
     2881 attgcgttgc gcccttcggc gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag
     2941 cttatcgacg cgcaaaacgg tcagccgttg ccgacggtgg atgtcatttt cccaattgtc
     3001 cacggtacgc tgggcgaaga tggttccttg cagggaatgc tgcgggtcgc caatttaccg
     3061 tttgtaggtt ctgatgttct ggcttcagca acctgtatgg ataaagatgt caccaaacgt
     3121 ctgctacgcg atgccgggct gaacattgcg ccatttatta ccctgacgcg cgctaatcgt
     3181 cacaacatca gttttgccga agtggagtct aaactggggt taccactgtt tgtaaaaccg
     3241 gctaatcagg gctcttctgt tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt
     3301 gccgtcgatc tggcgttcga gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt
     3361 cgtgagatcg aatgcgcagt tctgggcaac gacaatccgc aagccagcac ctgtggcgag
     3421 ctcgtactca ccagcgattt ctatgcctac gacaccaagt acattgacga agatggcgcg
     3481 aaagtggtag ttccggcagc cattgcacca gagatcaacg ataagatccg ggcgattgcc
     3541 gttcaggctt atcagacgtt gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc
     3601 ccagagaacg aagtggtgat caacgagatc aacacactgc ctggcttcac caacatcagt
     3661 atgtatccga agctgtggca agccagcggt ctgggttaca ccgatctgat cacacatttg
     3721 attgagctgg cgctggagcg tcacgctgcg gataacgcat tgaaaaccac aatgtaatat
     3781 tttaacgccc gataatattc ttattgggcg ttttcagttt cttcatcgcg acgacgaata
     3841 atcaaccccg ccagccagaa actaatcacc cacgtcacca gaccgacggc ataggtctgc
     3901 caaccttttg cttcaaatcc cagtaccccc accacgccat taagaatgaa tataaggcca
     3961 atggcgaacg catagtagtg ccagtcacgg cggattttta caggcagatt catggcagct
     4021 ccgttaacga aaaacaacat ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac
     4081 attttcgtaa ttgttaataa atttaactta aggttgccat tttgtgactc acaacgaaga
     4141 ttcacgcttc gggaatattc ccaacatcaa tttaccatga ttctgatatt gacaatctaa
     4201 atgacctgtc agacttgcca taagccgctg gtatcacacc agaagagaag cagatgactt
     4261 ccggaggttt ttatggctga tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc
     4321 aatgcctctt caacgcctgg caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg
     4381 gctggggcgc aattgctgaa cctgttagtg catgcgcccg gcgtctatga gcgtttaata
     4441 caggtccagg aaacaggtcg cccacgggtg gaaattggtt taggtgtcgg caccattttc
     4501 gggctgatcc cgtttttagc aggctgcctc atttttgcag tggtggcgct atggctgcac
     4561 tggcgacatc gccgccagta aaatttacgt tgccagatgc ggcctgaaac gccttatctg
     4621 gcctgacatt cctgtttgat cactgtttca tacaacgcgc ccgccgttcg tctacgcgct
     4681 tcgcaaacca ggccgtagtc aggttgcggg tgattttcgg gctttccagt tgaatgccag
     4741 gcaacatttc gcggggtagt gatttacccg ttttcgcttc ggcaagttga taaactttct
     4801 tgtacagcgc cgtctcctca aaagagaagc tgtcaccttt ctctaactga cggcgaattt
     4861 cgctgtcgct catccccagt tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg
     4921 gttctttact gtcgtaacga attaaatcac cgtccagtgc cagcttcacg ccgctggcct
     4981 tactgactgc gttctgaaac gcggcattac ggctggcgta ccagccagcg ttaaaatcag
     5041 caaagcggta tatcggtgcg ctatagctgg cgggataatt cagtaaatgg taagaaccaa
     5101 accacaaccc gccgcggcga ctgaagactt cctgacgaac tgtaccgtcc attttccacg
     5161 gatacccttt ggtatgctgt tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc
     5221 gcaccggatt aagcgaacca aacagcgtct gccccattgg taccatgctg atcagatcgt
     5281 caaaaatcgc gctcaattgc ttttctgtac gcaccgaatc taaccgttcg ctataacttt
     5341 tgccgtttgg cgatttgatt tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc
     5401 gttcagcacg acggtcaatc tcttgccagg cgatcttgct taagcccgga acggccggat
     5461 ctgcctgata atttgactcc tgctgcgcca ccgccagcac cgagcagacg ttttccagcg
     5521 tcggtgccag cccctgactt tcaaaggtgg tggcgagatc tttcgcccag gcatcgcggt
     5581 ctttcacgct cgcaggcatc ttctgccgca cgacgctcgc tacatctatc gctttttcgc
     5641 cctttttcaa cggttgcgga gcctgactac tacaccccgc taaaaccaga accgccaaca
     5701 atgacagcga agatagatga ttgacgcgcg acattacttc tcctttttag ctcaaggtat
     5761 gggttacttc ctgaattttg tcaccatcca gctcatgttc aaagctgcgc agacgtttgt
     5821 agatagacat caactcaacc agtgtggtcc atgagttaat caggtactgg aaagcaccgc
     5881 gaacctgacc aaaaacgttg gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa
     5941 caattgacgg aaacagcaag aacaaaccga aaacgttatc gacctgcaaa tagagaatgc
     6001 gggcgatgtt gaaatacata tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc
     6061 taaacagctc gcgtaccgta ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca
     6121 gctctttacg gtaggcggcc tctactcgct ggtttttaaa ctccagcccc ggcagtttga
     6181 tccctaccac tgccagcaat ccggtcccca tcagcgacca gacgatagcg gcaatcacca
     6241 gaccatacgg aatgtgcccg ataatcggca gctccggcac atgcgcggag agcgttacca
     6301 gcaccggcag gaaggcgatc aacgtcatga tggcgttgat aaaactgacg cccatattct
     6361 ccagcgttga agcaaaacgc atggtgtctt cctgcacacg ctgtgcggca ccttcgatat
     6421 gacgcagttg ttgccagttc gccatgtaat attcgttcat cgccgtacgc cagcggaaca
     6481 cgtagtgact gacaaagaag ttgttcagta cactgatcac caccgcgatc agcgcaatcc
     6541 ccagaaagac gcccacttcg cggtaaaatt gttcgatagt gactttatgc ggcgaactta
     6601 gcgcggtttg aatcagatca tagaacggcg cataccaggc gttgacggcg accccgactt
     6661 ccaccaaaaa ccaagtgacg aagataatca gcgcagtacc gagtattgac cagtattgcc
     6721 aacggtgcgg gctgtagata aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt
     6781 aagcgtaaaa aatcaggaaa tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc
     6841 cggaagcgcc ggtgatacgt gccacccagt cacccccacc ggcttgccag aagataacgg
     6901 caatcaatgc ccaaacaaag gccgagagaa aaaacgttcc cggctttggg aaaaaagact
     6961 taaacatatg cactcctgct aacttcttat cgttttaaca actgctgtgt attaaccgca
     7021 tgaccgctac aaagcgtcag ccaaatgaaa ccgcccattg tgacccatag caaggcgctt
     7081 agttcgccag cctgaccata agaattgttt cgacggattt cacaaaccaa agattgttta
     7141 cataagcgga ttaacacgcg caggatcaca aatgcaggca gtggtgagca ggaaaaagat
     7201 cgtgtttacg cgccgttacg tttttaacac tattattgca ctaattcgcc ctttgcaatc
     7261 catcaatgag tagtataaat acgctcagtt accttcattc aatctatgga caccaccgtt
     7321 gaaacgtagt ctgctttttt ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc
     7381 ggcgcagccg atcactgaac cggagtttgc ctctgatatt gtcgatcgtt atgccgatca
     7441 tattttttac ggcagcggtg ccacggggat ggcgctggtt gttatcgacg gtaaccagcg
     7501 cgtctttcgt agttatggcg aaacgcgacc tggtaataac gttcgcccgc agctggattc
     7561 ggtcgtgcgt attgcttccc tcaccaaact aatgaccagt gaaatgctgg tgaaattgct
     7621 cgaccagggg accgtaaagt taaacgatcc gctaagtaaa tatgctccgc caggcgcacg
     7681 cgtgccgacc tacaatggga cgcccattac cctagtgaat ctggcaaccc ataccagcgc
     7741 cctgccccgt gaacagcccg gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg
     7801 cgagcaacgc tggaaatacc tttctacggc gaagctgaaa gccgcgccag gcagtcaggc
     7861 ggcgtactct aaccttgcgt tcgacctact ggcagatgca ctggcgaatg cgtccggcaa
     7921 gccttatacc cagttgtttg aagagcagat tacccatcca ttggggatga aagacaccac
     7981 ctacaccccc tcaccggatc agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc
     8041 gtgcaataac acgctggcgg caattggcag cggcggcgtc tattccacgc ctggcgatat
     8101 gatgcgctgg atgcagcagt atttgtcatc tgatttttat cagcgcagta accaggcaga
     8161 tcgcatgcag acgttgattt atcagcgcgc gcagtttacg aaagtgattg gcatggatgt
     8221 ccccggcaaa gcggatgcgc ttggtttagg ctgggtatat atggccccga aagagggtcg
     8281 tccgaggatt attcagaaga caggcggtgg tggcggtttc attacctata tggcgatgat
     8341 cccgcagaag aatattggcg cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa
     8401 aaatatgagc gatggcatca atgatctggt gaccgagcta agcgggaata aaccgttggt
     8461 tatccccgca tcctgattaa tactctgaag gcaaacggtt gatagcgacc agtttgcctt
     8521 tattcatttc gatatagccg cctttacgca aagcagcgag aacttctgcg acgacagaac
     8581 gcgatatgcg tgtacgttga tggatataat tcatcacacc aatacgtgat cgcaattctt
     8641 cattccagtc aatcattgat aataatgttg cgcgggtttg tttggctggt agatgatatc
     8701 ccgtacaatt tccttctgat attaatttgt atggtatatc gtttttcatt aaaccatcag
     8761 ccagccccat aatataagga gcctgggtaa taccgataag tacgttttct tctctacgca
     8821 gagagataac tccttctaaa ataacgaaag tatcttcgtt attgacatca ctggaaaata
     8881 taacttgctt ttcattatta aactcgaagc gcgtaccgta tctggacaaa catttatcga
     8941 gcttaccaaa ttcctgaaga ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa
     9001 tgcccgtcaa atccttgacg ggcattattt agattaaatt
//
LOCUS       DADWTH010000134         9011 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.136, whole genome shotgun sequence.
ACCESSION   DADWTH010000134 DADWTH010000000
VERSION     DADWTH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.136"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(96..1472)
                     /gene="trkA"
                     /locus_tag="JNY02_003559"
     CDS             complement(96..1472)
                     /gene="trkA"
                     /locus_tag="JNY02_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBB9434952.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1494..2783)
                     /gene="rsmB"
                     /locus_tag="JNY02_003560"
     CDS             complement(1494..2783)
                     /gene="rsmB"
                     /locus_tag="JNY02_003560"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBB9434953.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2829..3776)
                     /gene="fmt"
                     /locus_tag="JNY02_003561"
     CDS             complement(2829..3776)
                     /gene="fmt"
                     /locus_tag="JNY02_003561"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBB9434954.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3791..4300)
                     /gene="def"
                     /locus_tag="JNY02_003562"
     CDS             complement(3791..4300)
                     /gene="def"
                     /locus_tag="JNY02_003562"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBB9434955.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4448..5572
                     /gene="dprA"
                     /locus_tag="JNY02_003563"
     CDS             4448..5572
                     /gene="dprA"
                     /locus_tag="JNY02_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBB9434956.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5544..6017
                     /locus_tag="JNY02_003564"
     CDS             5544..6017
                     /locus_tag="JNY02_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBB9434957.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6046..6588
                     /locus_tag="JNY02_003565"
     CDS             6046..6588
                     /locus_tag="JNY02_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBB9434958.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6593..7165
                     /gene="tsaC"
                     /locus_tag="JNY02_003566"
     CDS             6593..7165
                     /gene="tsaC"
                     /locus_tag="JNY02_003566"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBB9434959.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7170..7988
                     /gene="aroE"
                     /locus_tag="JNY02_003567"
     CDS             7170..7988
                     /gene="aroE"
                     /locus_tag="JNY02_003567"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBB9434960.1"
                     /translation="METYAVFGNPIAHSKSPLIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7985..8242
                     /locus_tag="JNY02_003568"
     CDS             7985..8242
                     /locus_tag="JNY02_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBB9434961.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8218..8772)
                     /locus_tag="JNY02_003569"
     CDS             complement(8218..8772)
                     /locus_tag="JNY02_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBB9434962.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2256 a         2189 c         2248 g         2318 t
ORIGIN      
        1 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
       61 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
      121 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
      181 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
      241 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
      301 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
      361 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
      421 tgtggtgaaa ttgcaatatc gatcacgctc ccctgaacca gatccacata agcgcgacgc
      481 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
      541 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
      601 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
      661 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
      721 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgtttatac
      781 ggtttttcca gtcgctgtaa ttcactcatc accgcgcgga tatgctgtga agcggcaata
      841 aagaacactt catcaccagc ttcaacaatg gtcgaacctt gtggacgaat ggggcgatcg
      901 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
      961 agtgcattac caatcagcgg gccgccataa taggctttaa ctacagcgag gctgacttta
     1021 ccctcagcga agttcaccac ctgcaatgcg cctggatact caatcagtcg gtaaatatta
     1081 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
     1141 agcttatcgg catcgcgtac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
     1201 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
     1261 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
     1321 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
     1381 gtatcgacaa cagtaatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
     1441 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
     1501 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggctg attttgttta
     1561 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
     1621 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
     1681 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
     1741 tccgggatat cgcgatcgcg acgtaaccat ttaatatctg gatggcgacg aatcaccccc
     1801 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
     1861 gaagggtaac ggccatcacc ttgtttcacg gttgccttca taccaaggcg ttttaaattg
     1921 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
     1981 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc caagatgtgt
     2041 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
     2101 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
     2161 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccag caatgcaagc
     2221 cagctgtcgc gggaatgatg cgtacgatta atacgcagcc acattggcgg acgctggtta
     2281 ttggcttcgg cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
     2341 caggaaggat gcagataacg tgcatcacgg gtattaaact cggctaataa ctcttcttgc
     2401 cgacgctgga actggcgtaa tacaccgtta atcaaccctt taagttgcgg acgcttaatt
     2461 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
     2521 agttgataca aaccaaccat aatcaggtaa tgcacagtgc gctgtttgcc ggtcatcgga
     2581 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
     2641 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
     2701 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
     2761 cgtaaattac gttgtttttt cataagtata aaaatgctcg gcaagaccgg gcttagaaga
     2821 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
     2881 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
     2941 tcaccagtcg caacctgaat gccttgtttg ttggcttcaa ggatcgttcc tggtgcagcg
     3001 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggctgtcc ttcaatttcc
     3061 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg tgctgccgaa
     3121 agtgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
     3181 tcgtcctgaa cttctggttt cgccgtgccg tctgccaatt gtttcaacgt ggtgataagc
     3241 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
     3301 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
     3361 atggttacac cagtttctgc atcacccgcc catagtgagc gttggattgg cgcagcaccg
     3421 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
     3481 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcaaatcg
     3541 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
     3601 tcttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
     3661 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
     3721 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
     3781 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaattc
     3841 gttgttgttt cagcggtgac agataatcca taaacagttt gccaaccagg tgatccatct
     3901 catgctgaat acagatagct aacagaccct ctgcttccag ttcaaatggt ttaccgtcgc
     3961 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
     4021 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
     4081 ttaacaccag tcgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
     4141 tatcaacctg ggttgccgct aggccaatac cttcttctgc gtacatcgtc tcgaacatat
     4201 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
     4261 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
     4321 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
     4381 cttgattgac agcatcactg accaatcgca aagattgcta aggcttgctt atgacaggga
     4441 gataaggatg gtcgatacag aaatttggct gcgtttaatg agcatcagca gcttgtacgg
     4501 cgatgatatg gtccgtatcg ctcactggct ggcaaaacag tcgcatattg atgcggttgt
     4561 attgcagcaa acagggctta cattgcggca ggcacaacgc tttctttcat ttccacgaaa
     4621 gagtatcgaa agctcacttt gttggttgga gcaacccaac catcatttaa tccctgcgga
     4681 cagcgaattt tatcctcctc aacttcaggc gacgacagat taccccggcg cactgtttgt
     4741 tgaaggagaa ctgcacgcgc tgcattcatt tcagcttgcc gtagtgggga gtcgggcgca
     4801 ttcatggtat ggcgagcgat gggggcgatt attttgcgaa actctggcga cgtgtggagt
     4861 gacaattacg agtggactgg cgcgtggaat cgatggtgtg gtgcataaag cggccttaca
     4921 ggtaaatggc gtcagcattg ctgtattggg gaatggactt aatatcattc atccgcgtcg
     4981 ccatgcccga ctggctgcca gtctgtttga acagggtggc gctctcgtct cggaatttcc
     5041 cctcgatgtt ccaccccttg cttacaattt cccacgaaga aatcgcatta tcagtggtct
     5101 aagtaaaggt gtactggtgg tggaagcggc tttgcgcagt ggttcgctgg tgacagcacg
     5161 ttgtgcgctt gagcaggggc gtgaagtttt tgccttgcca ggtccaatag ggaatccggg
     5221 aagcgaaggg cctcactggt taataaaaca aggtgcgatt cttgtgacgg aaccggaaga
     5281 aattctggaa aacttgcaat ttggattgca ctggttgcca gacgcccctg aaaattcatt
     5341 ttattcacca gatcagcaag acgtggcatt gccatttcct gagctcctgg ctaacgtagg
     5401 agatgaggta acacctgttg acgtcgtcgc tgaacgtgcc ggccaacctg tgccagaggt
     5461 agttactcaa ctactcgaac tggagttagc aggatggatc gcagctgtac ccggcggcta
     5521 tgtccgattg aggagggcat gccatgttcg acgtactaat gtatttgttt gaaacctata
     5581 ttcacactga agctgagttg cgtgtggatc aagacaaact tgaacaggat cttaccgacg
     5641 caggttttga gcgagaagat atctacaatg ccctgctatg gctggaaaaa cttgctgatt
     5701 atcaggaagg gttggcagaa ccgatgcaac tggcctcgga tcctctctcc atgcgtattt
     5761 atacaccgga agagtgtgaa cgactggatg ccagctgccg tgggtttctg cttttccttg
     5821 agcagattca ggtgctcaac cttgaaactc gtgaaatggt gatagagcga gtgctcgcgc
     5881 tggataacgc agagttcgaa ctggatgatc tgaaatgggt gatcctgatg gtgttgttca
     5941 acatcccggg ctgcgaaaat gcgtaccagc aaatggaaga attactcttt gaagtgaatg
     6001 aaggtatgct gcattaatca tttctttaat tcagcataag ttgttatggc aaaatcagca
     6061 ctgttcacgg tgcgtaataa tgagtcctgc ccaaagtgcg gggctgaact ggttattcga
     6121 tccgggaaac acggtccgtt tcttggatgc tcacagtatc cggcgtgtga ctacgtccgt
     6181 cctctgaaat cttcagcgga tggacatatt gtcaaagttc tggaggggca ggtttgcccc
     6241 gtatgtggcg caaatctggt attacgccag ggacgctttg gtatgtttat tggttgcagt
     6301 aactaccctg aatgcgaaca taccgaactt atcgataaac cggacgaaac agcaattaca
     6361 tgcccccaat gtcggacggg ccatctggtc cagcgccgct cccgttatgg caaaacattt
     6421 cactcctgtg atcgctaccc ggagtgtcaa tttgccatta acttcaaacc aatagctgga
     6481 gaatgccctg agtgtcatta tccgctactc atcgaaaaga aaaccgcgca gggtgtaaaa
     6541 cacttttgtg ccagtaaaca atgtggaaag ccggtttcgg cggaataata acgtgaataa
     6601 taacctgcaa ggagacgcta tcgcagctgc gatagatgtc ctcaatgaaa aacgtgtcat
     6661 cgcctatcca acggaagccg ttttcggtgt cgggtgcgat cctgatagcg aaacagcagt
     6721 gatgagactg ttggagttaa aacagcgtcc ggttgataag gggctgattt taatcgcggc
     6781 aaattacgag cagcttaaac cctatattga tgacaccatg ttgactgacg tgcagcgtga
     6841 aaccattttt tcctgctggc caggtcctgt cacctttgtc tttcccgcgc ctgcgacaac
     6901 accgcgctgg ttgacgggcc gctttgattc gcttgctgta cgagtcaccg accatccgtt
     6961 ggtggttgct ttgtgccagg cttatggtaa accgctggtt tctaccagtg ccaacttgag
     7021 tggattgcca ccttgtcgaa cagtagacga agttcgcgca caatttggcg cggcgttccc
     7081 ggttgtgcct ggtgaaacgg gggggcgttt aaatccttca gaaatccgcg atgccctgac
     7141 gggtgaactg tttcgacagg ggtaacataa tggaaaccta tgctgttttt ggtaatccga
     7201 tagcccacag caaatcgcca ctcattcatc agcaatttgc tcagcaactg aatattgaac
     7261 atccctatgg gcgcgtgttg gcacccatca atgatttcat caacacactg aacgctttct
     7321 ttcgtgctgg tggtaaaggt gcgaatgtga cggtgccttt taaagaagag gcttttgcca
     7381 gagcggatga gcttactgaa cgggcagcgt tggctggtgc agttaatacc ctcaagcggt
     7441 tagaagatgg acgcctgctg ggtgacaata ccgatggtgt aggcttgtta agcgatctgg
     7501 aacgtctgtc ttttatccgc cctggtttac gtattctgct tatcggcgct ggtggagcat
     7561 ctcgcggcgt actactgcca cttctttccc tggactgtgc ggtgacaatc actaatcgga
     7621 cggtatcccg cgcggaagag ttggctaaat tgtttgcgca cactggcagt attcaggcgt
     7681 tgggtatgga cgaactggaa ggtcatgagt ttgatctcat tattaatgca acatccagtg
     7741 gcatcagtgg tgatattccg gcgatcccgt catcgctcat tcatccaggc atttattgct
     7801 atgacatgtt ctatcagaaa ggaaaaactc cttttctggc atggtgtgag cagcgaggct
     7861 caaagcgtaa tgctgatggt ttaggaatgc tggtggcaca ggcggctcat gcctttcttc
     7921 tctggcacgg tgttctgcct gacgtagaac cagttataaa gcaattgcag gaggaattgt
     7981 ccgcgtgaat caggccatcc agtttccgga cagggaagag tgggacgaga ataaaaaatg
     8041 tgtatgtttt cccgctctcg tgaatggtat gcaactgaca tgcgcgatct ctggcgagag
     8101 tctggcgtat cgctttactg gagatacgcc agaacagtgg ttagcgagtt ttcgtcagca
     8161 tcgctgggac ctggaagaag aagcggaaaa cttaattcag gaacaaagtg aagatgatca
     8221 aggctgggtc tggttaccct gatccagata ttcgtccttc catttcacgt aattattcgc
     8281 ggaatagcgt aacccagcct tctcttcatc acttaacggg cggatctgtt tgacggggct
     8341 accgagatac agatatccgc tctccagccg tttattttgt gggaccagac tacccgcacc
     8401 aatcatcaca tcatcttcta ctattgcgcc atcaagtaaa attgagccca tcccaaccaa
     8461 aactcgattg ccaatggtgc agccgtggag catcaccttg tgaccaacag tgacatcttc
     8521 gccaatggtt aatgggttgc catctgggtt gtacgaggat ttatgagtga catgcaacat
     8581 actgccatcc tggatattgg tgcgtgctcc gatctgtaca taatgtacat ctccacgaat
     8641 cacaacgagc ggccagatcc ccacatcatc agccagacga acgtcaccaa tcacgacact
     8701 gctatcgtcg atcattacgc gctgaccgat ttgtggaaaa agatcgcggt atgggcgtaa
     8761 aacatcagac atacttacct cagcaataaa tgatttacta atgactttgg gggcattatt
     8821 ggccttgtgc aagtctttta gtatgcaaaa aagcaccgtt ttgtgtgcga ttgcagcaaa
     8881 aagggtgaaa aaacaacaaa cagaaaaaaa gatcaaaaaa atacttgtgc aaaatattgg
     8941 gaaccctata atgcgcctcc gttgagacga caacgtgaaa cacttcacag gatggtcgga
     9001 acaacgaaga g
//
LOCUS       DADWTH010000135         8993 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.137, whole genome shotgun sequence.
ACCESSION   DADWTH010000135 DADWTH010000000
VERSION     DADWTH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.137"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNY02_003570"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNY02_003570"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBB9434963.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKLWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNY02_003571"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNY02_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBB9434964.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="JNY02_003572"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="JNY02_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBB9434965.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="JNY02_003573"
                     /pseudo
     CDS             complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="JNY02_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNY02_003574"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNY02_003574"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBB9434966.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="JNY02_003575"
                     /pseudo
     CDS             complement(7822..8133)
                     /locus_tag="JNY02_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNY02_003576"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNY02_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBB9434967.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQFCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JNY02_003577"
     CDS             complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JNY02_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBB9434968.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGATVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2253 a         2326 c         2133 g         2281 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccaga gtttggtacg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ctgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaacagag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg gggtcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaatcag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccctccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtcgct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctaccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctatgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaaa attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgtag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta tta
//
LOCUS       DADWTH010000136         8895 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.138, whole genome shotgun sequence.
ACCESSION   DADWTH010000136 DADWTH010000000
VERSION     DADWTH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.138"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..2886
                     /locus_tag="JNY02_003578"
                     /pseudo
     CDS             1..2886
                     /locus_tag="JNY02_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="JNY02_003579"
     CDS             complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="JNY02_003579"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBB9434969.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3978..5330
                     /gene="yegD"
                     /locus_tag="JNY02_003580"
     CDS             3978..5330
                     /gene="yegD"
                     /locus_tag="JNY02_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBB9434970.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="JNY02_003581"
                     /pseudo
     CDS             complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="JNY02_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7279..8041)
                     /locus_tag="JNY02_003582"
                     /pseudo
     CDS             complement(7279..8041)
                     /locus_tag="JNY02_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
     gene            complement(8038..8697)
                     /locus_tag="JNY02_003583"
     CDS             complement(8038..8697)
                     /locus_tag="JNY02_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBB9434971.1"
                     /translation="MSEQITFATSDFASNPELRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2168 a         2358 c         2324 g         2045 t
ORIGIN      
        1 ggttgtcctg ctgatgcccg gagacgatcc tctcagggca tttttgatat gggtactgtc
       61 agaatccatc ggcgctctgg cactggtgcc gctgggattg ttatttaaac cacactatct
      121 gctgcgccat cgcaacccac ggttgctttt tgagtcgctg ctcacgttag ccatcacact
      181 gacgttaagc tggctttcga tgctgtatct gccgtggcct tttactttca ttattgtgct
      241 gttgatgtgg agcgccgtgc gcctgccacg aacggaagcc tttttgatct tccttaccac
      301 ggtgatgatg gtgtcgctga tgatggccgc ggatccctcc ctgcttgcta cgccgcgtac
      361 gtacctgatg agccatatgc cgtggctacc gtttttgctg atcctgctgc ccgccaacat
      421 catgaccatg gtgatgtatg cctttcgtgc ggaacgcaaa cacatttccg aaagcgaaac
      481 ccgctttcgg aacgcgatgg aatattccgc tatcggtatg gcgttagtgg gcaccgaggg
      541 acaatggctg caatccaaca aagcactctg ccagtttctc ggttacagtc aggaagagct
      601 gtgcggactc acctttcagc aactgacctg gccggaggat ctcaataaag atctccaaca
      661 ggttgaaaag ctgataagcg gtgaaataaa cacctattca atggaaaaac gctactacaa
      721 ccgcaatggc gatgttgtct gggcgttgct tgccgtctca ctggtgcgcc acacggatgg
      781 cacgccgctc tattttatcg ctcagattga agacattaac gagctaaaac gcaccgaaca
      841 ggtgaatcag caactgatgg agcgcatcac gctggctaac gaagcgggcg ggattggcat
      901 ctgggagtgg gagctgaagc cgaatatttt tagctgggat aagcggatgt tcgagctgta
      961 tgaaattcct ccgcatatca aaccgaactg gcaggtgtgg tacgagtgcg tgctgccgga
     1021 agatcgccag catgccgaaa aagtgattcg ttgcaatcac gctcgccctt taagctggaa
     1081 tttcgcattg ccgtgaaaga tggcatccgc catatccgcg ccctcgccaa ccgggtactg
     1141 aataaagaag gcgaagtcga acgcctgctc ggcattaata tggatatgac cgaggttaaa
     1201 cagcttaacg aggcattgtt tcaggaaaaa gagcgcctgc acattacgct ggattccatc
     1261 ggtgaagccg tggtctgtat tgatatggcg atgaaaatta cctttatgaa tccagcggcg
     1321 gagaagatga gcggctggac gcaggaagaa gcattaggtg ttccgctcct gacggtgttg
     1381 catattactt ttggcgacaa cggaccatta atggagaaca tttacagtgc cgacacctca
     1441 cgttccgcga ttgaacaaga tgtggtgttg cactgccgga acggcggcag ttacgacgtg
     1501 cattacagta ttacgccgtt aagtactctg gacggcagca atataggctc ggtactggtg
     1561 attcaggacg tcaccgaatc acgcaaaatg ctgcgccagc tgagctacag cgcctcccat
     1621 gatgcactga cgcatctcgc caatcgcgcc agttttgaga agcaactgcg tatcctgctg
     1681 caaacggtaa acagtacaca tcagcgacat gccctggtgt ttatcgatct tgatcgcttt
     1741 aaagcggtga atgacagcgc cgggcatgcg gcgggtgacg ctttactgcg cgaactggcg
     1801 tcgttgatgc tgagtatgct gcgctccagc gacgtgctgg cgcgactcgg tggtgatgaa
     1861 tttggtctac tgttgccaga ctgtaatatc gaaagtgcgc gttttatcgc tacacgcatt
     1921 atcagtgccg tgaatgatta tcactttata tgggaaggcc gtgtgcatcg ggtaggtgcc
     1981 agtgccggga ttaccttgat tgatgacaac aatcatcagg cggcagaagt gatgtcgcag
     2041 gctgatatcg cctgttatgc ctccaaaaat ggtggacggg gccgggtgac ggtttacgaa
     2101 ccacagcaag ctgccgcaca tagcgaacgg gcggcgatgt cgcttgatga acagtggcgg
     2161 atgattaaag agaatcagtt gatgatgatc gcccacggtg tcgcttcgcc gcggatcccg
     2221 gaagcgcgta atttgtggct gatttcactt aagctctgga gttgcgaagg cgagattatt
     2281 gatgaacaaa aatttcgtcg tagcttcagc gatccggcac ttagccatgc tcttgaccga
     2341 cgggtattcc acgaattttt ccagcaggcc gcaaaagcgg ttgccagtaa aggcataagc
     2401 atcgccctcc ccctttccgt tgccggtttg agtagcgcca cgctggtgaa tgatctgctt
     2461 gagcagctgg aaaatagccc tctaccacca cggttattac atctgattat tccggctgaa
     2521 gcgattttag atcacgcaga aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg
     2581 ctcagccagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat
     2641 tacctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg
     2701 ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat cgccgggcca
     2761 gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct gatttatggt
     2821 gatgtgattg ccgatgccca accgctggat ttgctggtga atagcagtta tttcgcgatt
     2881 aactgagaga gcgtttgccc attgcctgat gcgatgctaa cgcgtcttat caggcctaca
     2941 aatcgctcat tccgcaggcg ggataaggcg ctcgcaccgc ttccggcgac caacgtcatg
     3001 cttcgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa gaacgccagg
     3061 gcttccagcg ctcggcataa cggcggattt gtgccggtgt catccccaga aatcgctgtt
     3121 taatcagata atcatccggc agaaaaacat ctttcgcctg ccagccgcgc aaggcgaaat
     3181 aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc gcctgctcca
     3241 catcgcccgg tattgtcatt ggtaaggtgc cctccagcgc cgcatttgcc agatgaatca
     3301 gcgcctctgc ccgtttcaac ggcataccta acgcttttaa tgcctgcggg tcggctgctg
     3361 ccagccgctg aggcgtgggg aagcagatat actccggaaa atcatccagc cgttcgccat
     3421 aaagctgtgc cactctggcg gtcaattttg ccgccatcgc cacgctcacc agttggccta
     3481 aaatcgcccg cacgccctgc tcaaaagcat caacactgcc gggtaaacgc aatccgggcc
     3541 gcgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat tgcagatcaa
     3601 gcaggcggct cattttcgcc agacactcag cggcaacggg ttctaaacct gcacttaaat
     3661 ttatgtgcag agtatggcgg gctatatccg gaatagcagt caccacgccg cgatattcgc
     3721 ccactgccag actacgggca taataactgt ccgcgaccgt ttccacacca ctcaccgcac
     3781 gggcggcgag aaatcccaac atccacgacc agtcatacgg cggctgccag ttcaggatgt
     3841 acatcgcatc tccttattca tccgctttca gcataaacgt tattcagacg ttacgctttg
     3901 ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc cttcttcacc
     3961 ggcatgggga ttatttcgtg tttattggtt ttgattacgg tacagcaaac tgttcagtgg
     4021 cggtcatgcg tgacggtaaa ccgcatttgc taaaaatgga aaacgacagc acgctgctgc
     4081 cttcaatgct ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac cgccatcatg
     4141 acgttccggc agacgacgat gaaacgcagg cgttgttacg acgggcgatt cgttataacc
     4201 gcgaagaaga tatcgatgtt acagcgaaaa gcgtgcagtt cggtctttcc tcactggcac
     4261 agtacattga tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg
     4321 ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc gcaatgatgt
     4381 tgcacattcg ccagcaggcg caggcgcagc tgccagaagc aattactcag gcggtgattg
     4441 gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag gcgcagggga
     4501 ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag tacgagccgg
     4561 tagcggctgg gctggattac gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg
     4621 atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag tggcgttcgc
     4681 gtctcgatcg tgaagccagt ctgctgggtc acagtggttg tcgtattggc ggtaacgatc
     4741 tggatatcgc cctagcgttt aaaaacctga tgccactgct gggtatgggt ggcgaaaccg
     4801 aaaaaggcat cgccctgccg atcctgccgt ggtggaatgc ggttgccatc aacgacgttc
     4861 ctgcacagag tgatttctac agtagtgcca acggtcgtct gcttaacgat ctggtacgcg
     4921 atgcccgcga accggaaaaa gtggccctgt tacagaaagt ctggcgtcag cgtttaagct
     4981 atcgcctggt gcgtagcgca gaagagtgca aaattgctct ttcaagcgta gcggaaaccc
     5041 gcgcctcact gccgtttatc agcaatgaac tggctacgct gattagccag cgagggctgg
     5101 aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg gccctggata
     5161 acgcccagga aaaaccggac gttatctacc tgaccggcgg tagcgcccgt tctccgctga
     5221 ttaaaaaagc gctggcagaa cagttgccgg gcattccgat tgcaggcggc gatgactttg
     5281 gctccgtcac cgccgggctg gcacgctggg cggaagtggt atttcgttaa aagaaaatgg
     5341 ctcagcagcg gcttaaatca gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg
     5401 gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc aactctgtcg
     5461 cgccgacagt caacgtcgat tccagccgat gtcgtttggc ggccattttc gccattaccg
     5521 cctgtctgtc tgctggcgtt accgcttcgt tcggcctgaa cacaaaacgg cgttcacagc
     5581 tcgctttcca gtcgatgacc gcctgggtca gatgatcacc aaacccttta acttgcttaa
     5641 cgccacgacg ggtaacacct gctgctgttt caataccaaa agaccataac gccgctttac
     5701 gcgcagggcc aacgccggga atagaggcaa catcaataaa aaatccctcc agaaacttct
     5761 acttctgccg ttcccttgca gtgtcatgaa gtgctgccag agcgagtttc tcttcttccg
     5821 gtaacccgag aatttcgtcc ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt
     5881 ccaggccgcc tacctgctgg atctggcgga ctaaatggtc atagtccatt ttcgcacgat
     5941 taaatgcctc acggcgttgc tgaacttctg ctttgtacgc tttgcttgtc agactgccaa
     6001 taatccagat agccgccagc acaggaacca aaataatata acgcggttct gcctgaaggc
     6061 cgcaaagcag cagcgataat gctgacagtg cgatctcaag cagaatgatg tattcgcgcc
     6121 gtaacaggcc taaaggaagc ggcctgccag tcggttggaa atgatcgggt aatggcagtt
     6181 gcaatgctgg cggtgctact gacgccatca ccatcgccca gacttttgcc agcacaaaat
     6241 ccccgccggt ggtaatgacc tcttcgccga gatcaataaa atagataacg ccttgattat
     6301 ccagcgtaca ccacaggcag tcggtcaaat gagcgggata aacatgcatt gccgaaacgg
     6361 gacatttctt taactgttgg cgcagagaat caagcgccgc taccctcgct ttagccgtcg
     6421 gacgcccggt tgccacgcca ctttccgtga atgcctgctg aaacatggct tcaacatcac
     6481 tcggtaacat cgatagcgga atcgatcgtg gcggcggttt taaaccacgt cgctgattat
     6541 ctgacgcgta ggcataacgg aaatgggtaa tatccgtctc cagcgcatta cccgcatcag
     6601 agataagcgg cacaccagaa taaggatgcc ggccaccaaa caagacgtga aaaatcagca
     6661 acgcaaggcc aaaattaccg tgattttctg tgcgttcaaa gccgacaaat gacgacaagg
     6721 tttgtagctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc agtgtgccat
     6781 tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac tttgctgtcg cgacctacca
     6841 taaaactgtt ctggtttacg tcacccacga catgcccgtg ctcgtgaacc gtagcaaaag
     6901 atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga gggtaacgtt
     6961 gacgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa acttttggca
     7021 tcataaaacc gataactttt ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga
     7081 cataattcaa caattgcgcg tcagctgtcg cagccataaa ggcaagtttg tcctgtttta
     7141 aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactca ttgatatcat
     7201 aaaccgcgcc ttcaccgcct ttgcccagtt cacggcctgg cgtaacgcac tcgccagtag
     7261 cagtatataa agtgggtttc atggtaacca cagcgccagt gccaacgttt tatcatcatc
     7321 cgtacgctcg tttaccgccg ggctggacaa aaactgcttt aacaattcag gtaataaatc
     7381 gagttgttcc tgcgttgctg acgccagtcc attgaaaaac ggggtaaaga aaggcacatg
     7441 cggagaatta tccaacatat ttaacgccag tcgctggata ccatccgtaa atgctgcaac
     7501 tttatgcaca cgctcagtgc tggtgaaagt ttccagtctg gaaacggcat cttcatcggt
     7561 aatgaagtgc gtcatattgg catattcacc caccatcggc gtaagcggca attgcagacc
     7621 gtgaccaaaa tccaccacca cgccgccatc gccaatctgc ataatcaagg tgccattagc
     7681 cgatgaaatc aatcccagaa acgtacaggc aaaatcgcga actgctaatt ctttggcttc
     7741 cgcctcggca ataaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcggttggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtagttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agagg
//
LOCUS       DADWTH010000137         8888 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.139, whole genome shotgun sequence.
ACCESSION   DADWTH010000137 DADWTH010000000
VERSION     DADWTH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.139"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..219
                     /locus_tag="JNY02_003584"
     CDS             <1..219
                     /locus_tag="JNY02_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HBB9434972.1"
                     /translation="ALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQ
                     LQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            246..1021
                     /locus_tag="JNY02_003585"
                     /pseudo
     CDS             246..1021
                     /locus_tag="JNY02_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(1085..1576)
                     /locus_tag="JNY02_003586"
     CDS             complement(1085..1576)
                     /locus_tag="JNY02_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBB9434973.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1678..2232)
                     /gene="ycdY"
                     /locus_tag="JNY02_003587"
     CDS             complement(1678..2232)
                     /gene="ycdY"
                     /locus_tag="JNY02_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBB9434974.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2256..2993)
                     /gene="ycdX"
                     /locus_tag="JNY02_003588"
     CDS             complement(2256..2993)
                     /gene="ycdX"
                     /locus_tag="JNY02_003588"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBB9434975.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3048..3986)
                     /gene="ghrA"
                     /locus_tag="JNY02_003589"
                     /pseudo
     CDS             complement(3048..3986)
                     /gene="ghrA"
                     /locus_tag="JNY02_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            4220..4307
                     /locus_tag="JNY02_003590"
     tRNA            4220..4307
                     /locus_tag="JNY02_003590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4254..4256,aa:Ser,seq:gga)
     gene            complement(4457..5299)
                     /locus_tag="JNY02_003591"
     CDS             complement(4457..5299)
                     /locus_tag="JNY02_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBB9434976.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELIDRLNDIIAKHYPD
                     MLAV"
     gene            complement(5384..5581)
                     /locus_tag="JNY02_003592"
     CDS             complement(5384..5581)
                     /locus_tag="JNY02_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434977.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(5593..6084)
                     /locus_tag="JNY02_003593"
     CDS             complement(5593..6084)
                     /locus_tag="JNY02_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434978.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(6081..6455)
                     /locus_tag="JNY02_003594"
     CDS             complement(6081..6455)
                     /locus_tag="JNY02_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBB9434979.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(6502..6876)
                     /locus_tag="JNY02_003595"
     CDS             complement(6502..6876)
                     /locus_tag="JNY02_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBB9434980.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(7039..7260)
                     /locus_tag="JNY02_003596"
     CDS             complement(7039..7260)
                     /locus_tag="JNY02_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBB9434981.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(7323..7799)
                     /locus_tag="JNY02_003597"
     CDS             complement(7323..7799)
                     /locus_tag="JNY02_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBB9434982.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7815..8300)
                     /locus_tag="JNY02_003598"
     CDS             complement(7815..8300)
                     /locus_tag="JNY02_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBB9434983.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(8391..>8888)
                     /locus_tag="JNY02_003599"
     CDS             complement(8391..>8888)
                     /locus_tag="JNY02_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HBB9434984.1"
                     /translation="TLQETRGGSGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEG
                     AYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRR
                     EDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETL
                     LESMR"
BASE COUNT         2193 a         2285 c         2255 g         2155 t
ORIGIN      
        1 gcgttagata actttactca ggccatccag tcacaaattt taggtgggct actgtcgaat
       61 attaataccg gtaaaccggg ccgcatggtg accaacgatt atattgttga tattgctaac
      121 cgcgatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc
      181 caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc ataaggaaaa
      241 taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga tgcttaaccg
      301 ccccgcctaa agaagccgcc agaccgacat taatgcctcg tgctcagagc tacaaagatt
      361 tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac attcaggacg
      421 aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg
      481 ccagggctta caaaacctgc ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg
      541 cacggttgcc attaataacc gaatcccgct gcaatcttta acggcggcaa atattatggt
      601 tgaaggttcg attattggtt atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata
      661 ttttgggatc ggtgcagaca cgcaatacca gctcgatcag attgccgtga acctgcgtgt
      721 cgtcaatgtg agtaccggtg agatcctctc ctcggtgaac accagtaaga cgatactttc
      781 ctatgaagtt caggccgggg ttttccgctt tattgactac cagcgcttgc tggaagggga
      841 agtgggttac acctcaaacg aacccgttat gctgtgtctg atgtcggcta tcgaaacagg
      901 ggtcattttc ctgattaatg atggtatcga ccgtggtctg tgggatttgc aaaataaagc
      961 agaacggcag aatgacattc tggtgaaata ccgccatatg tcggttccac cggaatcctg
     1021 acagataaaa atgccggaat taataatccg gcatttactg tttaagcgaa agcgtgagtg
     1081 cccctcacgc tttatttttg atttcctgct cacggtgagc cgtctttgca ctacgcgccg
     1141 ccagccacag gccgctgttt ttcattgcgt aaccaaatac cagcccaagc gccagcgaag
     1201 gtaacaccag tttccagtca ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc
     1261 ccggaacaaa tgaaagcagc agctgtttgg cctgaataca catcagaaac gcgacaatac
     1321 cggttatgac ataaccgaga atttccagat gtggtgccag tgcactaccg taaataatga
     1381 ccatcgccca caccacgcca ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc
     1441 caccttgcgg gcaagcaaag taggccgtac agcccaggaa gcccgcccag cttagtaagc
     1501 ccagggaaac agccacccat ccccagatac cggagagaat gcctgttgtg attgcaatag
     1561 agagaagtat gttcatgccg cgcatgatag cagaaaacgc ggccatgaat gagatcgagc
     1621 acacatttta tgcaacaaca aattatctgt tgcagttatg agtgacgatg atcacattta
     1681 ctcttcagaa tcttcttcca gctcgtccca cattgcacta atggcatcac gggttagcgg
     1741 tgccatggtg cgccagaaag gcgtggttgc atgggcctcc actttgccga ggaacgcacc
     1801 acaccagggt aacagatact cactgaacag tgtttccagt gcttcgctct catcttccgt
     1861 tgactgatct tccagccagg aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt
     1921 atccgccagt ggcatccctc gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc
     1981 ctcaacccat gcgctacgat atggcggcac ggcacattca tcgccgataa acaacgcatt
     2041 gtaatcggca gagacttgcg tcatatcaca gcttttctgt aaacgcgtca gtaactcatc
     2101 ctgctccagt ggccagttcg cagccagttt cccctcacga atcagagtaa acagcggcac
     2161 cagtaaagga tcttgcggtt ggcggtaata gagcgaaccc agcacacgac agaggataga
     2221 aaactcgttc attaatatat ttccattact agtaattaaa ggtctgcaaa ttccgcaatc
     2281 ggtgccatac cgcgagattc aaggaagttc agtaagcggc gcggagaaac attcaaaatg
     2341 cgctctggcg gaaaatctac cgcgtcgagg attttaagac actcttcaaa ttcccccatg
     2401 gtaaacgcgg tgtgagaatc cgagcctaat gccacccaac ctccagcatc acgtaccgct
     2461 gcagctaccg cacgacagtt gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta
     2521 ttgatttcca gcgccacctg atgtttcgcg gctgcttcag caacggcttt aacatctatt
     2581 tcatatttcg gatttccggg atggcttatt atatgcacat tgccgctggc gatagtggcg
     2641 atcatcgctt gtgtattggt cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg
     2701 gcaataatca gatccagcga gtcaaacatt ttaccgctgc agtcaatttc accatcaaca
     2761 tttttaatgt tagcttcaat gccgcgcagg atccctaccc catcaaccac tcgcggccag
     2821 atacgcatgt taatgaagtg ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg
     2881 gtgatcgcaa aaagtttaat gcccttttgt ttggcctggg caatgtaatc actcaatgtg
     2941 ctatatgcat gtgtgctggc aacggtatgc atatgaaggt cgacgggata cataactctc
     3001 tcctgtattc aattcccgcc aaggatagca ggaatcctga tgctttatta gtagccgcgt
     3061 gcgcggtcga cttgcccgca gaccctctcc cctttttcga gctgggcaat agtgcgagaa
     3121 atgtactcca cagcttcagc gggacgggta atcgcggcga catgtggtgt tatcgtcacg
     3181 cgtggatgtt gccagagcgg actttcaggc ggtaagggtt cacgattaaa aacatccagc
     3241 attgcgcctt taactttccc gctatccagc gccgcgagca ggtcatcttc cacaacatga
     3301 acaccacgcg ccaggttgag gagatacgcg ccatcaggta atttttcgag taattgttga
     3361 ttaataatgc cgacggtttc aggggtattc ggtaacaaat taatcaatac ccgacattgg
     3421 ctcagaaatg cagacagttc ttcccgtccg gcaaagcttt gcacgccagg ccacgattta
     3481 cgggttcgac tccagcaacg cagcggaaag cgctaggttt gcagactctg agcaacttta
     3541 cagcccagta cgcctgcgcc caaaatgccg atggtaaaat cttcccgatg atattcaggc
     3601 agcggttgcc aatgcgaact attttgctgg atgcgataat catcaaaacg tcgaaaccaa
     3661 tgcagcacct gactgacagc atattcctgc atttgctcgc ccataccggt atcttccagg
     3721 cgaaaaagtg gaacagaagg gttcagcatt tcagggtgtg cctgtagctt gctcaaaata
     3781 gaatcaacac cggccccgag tgcgaacacc gctttaagat cgcgccctgc cagcatttca
     3841 acaggaggat gccagactaa aacataatca gcagagtcat tatctccgct tttccatgcc
     3901 ctgactcttg cctgaggaat agctttgcgc agtgcctcaa tccaccattg ggtatcgaac
     3961 gttgggtgat aaaagatgat atccatactg actcccgaaa agcgttgtgc gaaatttatt
     4021 cgcacttatc gttatgatct acaaaggcca ccagcataac aaatccgtgg tcggtggcaa
     4081 aaaaagcaga tttcgcttat taaaaccaca cattgattga agtttgaata aacgcgcgat
     4141 tctttcaaaa agtttgttga cctcaggtca tgatttccct aaattagcgc ccgttccagc
     4201 aagacaggaa cgacaatttg gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt
     4261 gtgtatacgg caacgtatcg ggggttcgaa tccccccctc accgccagat tataagcgca
     4321 tgttattgtt taacatgcgc ttttttattt tcccttccag acagtatgcc atcgggtatg
     4381 ccatctgaaa ccggtttata tgccccgcga tcatatctta tcggacaatt tccgatcttt
     4441 gagcatactg gcaaagctat actgccagca tatcaggata gtgttttgcg ataatatcat
     4501 tcagtctgtc aatcagttct ggttttctga acgtgatatg cgcggagccc ttcttaaagt
     4561 atctgatgct gaacatctca tcttcatagc tctctttacc ctgaacagac tgaatgtgat
     4621 catccagacg aagggtgatg tttccccggt tgtcggggat tggtttaccg ctgaacaggt
     4681 gcaacattct ttccagatcg gcgagtcggt cagcctgttg tccggtgata agatgaaatc
     4741 cccaccggtc ccatctcacc agattgttga cgataatttt actgccaaat ttacaggggc
     4801 aattggtttt gtaattccag ttcagccctc tgaagacgtt gatcacccct cgctcaaaca
     4861 cctcatcctt attctggtgc agttgttcaa atgtgctcag aatgttcgct tcgctgattt
     4921 ccggaaaatt atcgtactcc agtgaccggt accatgtatc acgggcctgc gtatccatta
     4981 atgacagcat cccggaacgt tgcatcaggt cacgccagat ttcgcgatca agattccggg
     5041 taatggcttt cattgctgtt tcgggtttct ccatcagcca gcagccgcag cgaaaatcct
     5101 gtttcatggc ccagtccggg gcagttttac ctccgatgct ttccgtcagc accgagattt
     5161 cagccagttg tcggataagt gtgtcgattt gcaacagtgt actctttcgt acagccagaa
     5221 tgtggcgaat gctggttgag cagataacgt ccgtatgccc gttgaccact tccggacggg
     5281 agattgtgat atctgacata atcgatattc ctttttgcag ataaatgaaa acgccagccc
     5341 tgtgcaggct ggcgtcagag gaatgaagtc tggtgagtga gcttcaccgg ttctggtgca
     5401 gaagctgcag atgacgcagg gtacggacat cctcccgggc ctgttcgata cagggcaaca
     5461 gtgctgctga atctgtttta tcctcatcgt tgtcaaagat aattcccgat ccgcagtcga
     5521 tattgtcctg cagccacgca atcagaatat ccagcgctgt ttccgtggtt aatgatttca
     5581 tgttgttcct ggttaccagt cgaaagtggg taaacccggc aggcattcag cgctggcatc
     5641 cagatgaatg agactgacgc cataacgccg gatgagtgtg acgaccagac gacggaacgt
     5701 ttttgacagc cccaggcgtt taagccgcag aaccgggtat gaccacgcat cggtacgtaa
     5761 cagatatccg ctacctgtaa aatgaatcca ttctgattca ccgaagtcac tggtctggtg
     5821 tgacagcgag tacaaccagg cgttgtccgt ttccgtgata tgtgcggtac tgcagcgtat
     5881 gccggtaagg gtagcaaatg gtggcgtgca ttctgctgag gtctggtcag attcatcaac
     5941 aacacgaagg gagtagccgt tatcgcagac catgttaatc agttcagcga gctcaatacc
     6001 atcgacgtca acgacaatgc gccccatgtt cagtgcctgt acgttaacgc tgtcggcttc
     6061 cagtgtcagg gccagtttca tcatttcgcc tccggatact tacccaaggt aatgttattt
     6121 accgttctgt aattgtcgcg ggtcatcagg ccggtcgccc ggcgagcccg gaggatatca
     6181 atactgttta ttaactgaga gcggggacag gtgctgaatc ccggctggtc ggtacgcacc
     6241 agcgcgtatt tttccacgag aaaattaact gcatcacaca gtgaaatgcc tgcctcaata
     6301 tgctgctcaa tcacacgttc atcggcaaac ggtgtgtcat tcagtgtgag gccataatgc
     6361 tggtccagca gtcggatgag cagtgtctgc cagatggtga cgggagacgg gcagcgagat
     6421 acctcccgta catgtgtgtc ggataatgtt ttcatcttaa gttctctgta actggtagtg
     6481 ctaaaggtga gagcttcggg gttatgaggt ggttgcgagt gctggtgtcg gataaacagc
     6541 cagataaacg taaccacagg agccgagggt gtcggcttcg caggtcagcc cttttgcatt
     6601 cagcgtgacg gtatgctgat atcggggatt cagttcaccg ctggtgagca tgagttccag
     6661 ttgtttcatc agcagcggaa aggcctggtc cagatggtat gcatctgcgt cgctgaagcg
     6721 gcctctgata ccggcgcggt cggcaaggta atgcaggcgg ttaccctctt gcaccagacg
     6781 agcgccaaaa cagggcctca ctgtgcaggg aagcccccac caggggcggt cgtgattgtc
     6841 atcgggatga gttatcccgg agagtttgtc tgacacgata aaatcctctc tgaaaagtgg
     6901 ttaagaatgg tttaacgatg acaggtcagc cggcgcgctg accggtttcg gtgaagatgt
     6961 atgcattgat gagaatggcc tcatccacag cggcgtctga ggtcatctgg tcgtactcgt
     7021 ttcccgggtg acggaacatc agttcagggt gacactcatc agccggacat acgggccaaa
     7081 actgtcctta cggcgttctg caaacacggc aagaacgccg ggaatatcct gcacttcacg
     7141 accagtatac gcttcagcac tgccgtgcca gcgatactta ccggtacaga acggaaaaag
     7201 acgggatgtc ggatgttgtt ggtgaatacg catggcttca ccacgggtga taattttcat
     7261 aatgggatac ctctgaagac agaagataaa agtgaaaaca ggtgtgatgt ggttgtgaag
     7321 ggttaaagca gaccatgttc ggcaaaggag aaaacctggt tgccaccgac tatcagatgg
     7381 tccggcaccc ggatatccac caggcccagt gcctgtacca gacgttcggt gataagccgg
     7441 tctgccttac tgggtgtgac ttcaccggac gggtgattgt gcgccagcac cacggcagcg
     7501 gcattgtggt acagggcgcg tttaatcact tcccggggat gaacttccgt acggttgatg
     7561 gtgccggtga agagggtttc accggcaatc agctgattct ggttgttcag atacagcacc
     7621 cggaactctt cccgctccag tcccgccatg ttcagaatca gccattcccg tgccgcacgg
     7681 gtggaggtga aggccacgcc gggttcatga agatggcggt ccagggtttg cagggcccgc
     7741 agaatgagac tgcgctcgcc gggcgtcatc tctccgggca gaaaggaaag ctgctgcatt
     7801 gtccctgcct ccattcagtc aatgatgcgc ataatggcgc tgcattccgg atgctgcagg
     7861 gcgtaatccc gcagccggta atagtggacc gtcatggcat aacactccgt acgacaggca
     7921 tgatgactgt acgtcatcag acaggcagca ataccggcgg cttccgggct catttcagcg
     7981 cggttgccgt tcatggcatt gaacagtacc catgtttcgt catcatcgtc atccggttcg
     8041 ggtgccataa atgccccgcc gttgttcagg gtgtacagat tccagatacc accgcagtag
     8101 tcttcgcaaa gacggtccat ccagccgaag acacggggct ccagggtcac ccactgtgga
     8161 atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt catcagggac gggagtggca
     8221 accagctgag gtgggttatt ccctgatgca gtggttacgg aaacagaagg tgtggtggaa
     8281 ttatgcgaaa cggttgtcat gaggttattc cttataaaaa gtaaatgaat ggaagaagcc
     8341 ccggggcagg aacagatgtg agtcaggact gcactttcag tgaaacggca tcagcgcatg
     8401 ctctccagca gcgtttctgc catcacccac aatgcgcgat tgagcttaat gtcggtatca
     8461 atgctgtgaa tggcccgggt atggatacgt tttccttttg cactgcgacc ggaaatcccg
     8521 cctttcagca tattctcctg gatggtctga taagcactcc acaggtcctt accgtaatcc
     8581 tcccggcgtc gtggtgtcag aatgtcggcg gtggtgacgg gctgatgttc gtcaccataa
     8641 cggtaagtca gtgccgcctg tgccagcgcc tggcgtgccg gtggcggcag gaccagcgac
     8701 tgcatggcat cacgcttctc ctctatccgg tcaaaaacgc ccaccacctc gtaagcccct
     8761 tcaatgactc tgtccaccac atttccccgg tgtggaacac gcaattctcc cagagactga
     8821 ccgcagacca gtccattggt acaaatggca cgaaaatatc ctgaaccgcc ccgggtttcc
     8881 tggagagt
//
LOCUS       DADWTH010000138         8863 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.140, whole genome shotgun sequence.
ACCESSION   DADWTH010000138 DADWTH010000000
VERSION     DADWTH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.140"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(99..1178)
                     /locus_tag="JNY02_003600"
                     /pseudo
     CDS             complement(99..1178)
                     /locus_tag="JNY02_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            complement(1388..1831)
                     /locus_tag="JNY02_003601"
     CDS             complement(1388..1831)
                     /locus_tag="JNY02_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434985.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            2346..3188
                     /locus_tag="JNY02_003602"
     CDS             2346..3188
                     /locus_tag="JNY02_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9434986.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            3295..3558
                     /locus_tag="JNY02_003603"
                     /pseudo
     CDS             3295..3558
                     /locus_tag="JNY02_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4185..4730
                     /gene="cobU"
                     /locus_tag="JNY02_003604"
     CDS             4185..4730
                     /gene="cobU"
                     /locus_tag="JNY02_003604"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBB9434987.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4727..5470
                     /gene="cobS"
                     /locus_tag="JNY02_003605"
     CDS             4727..5470
                     /gene="cobS"
                     /locus_tag="JNY02_003605"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBB9434988.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSCDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5482..6561
                     /gene="cobT"
                     /locus_tag="JNY02_003606"
     CDS             5482..6561
                     /gene="cobT"
                     /locus_tag="JNY02_003606"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBB9434989.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6623..7558
                     /gene="ldtA"
                     /locus_tag="JNY02_003607"
     CDS             6623..7558
                     /gene="ldtA"
                     /locus_tag="JNY02_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBB9434990.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFASQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7614..7689)
                     /locus_tag="JNY02_003608"
     tRNA            complement(7614..7689)
                     /locus_tag="JNY02_003608"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7654..7656),aa:Asn,seq:gtt)
     gene            8016..>8863
                     /gene="nac"
                     /locus_tag="JNY02_003609"
     CDS             8016..>8863
                     /gene="nac"
                     /locus_tag="JNY02_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBB9434991.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAV"
BASE COUNT         2258 a         1971 c         2258 g         2376 t
ORIGIN      
        1 cacaggtctg tcgccccttc agttgccagt tcgctgtctt tacagacgta atcttttttc
       61 atgcgaccga aggcttccct gacaaaatca tcgacctagg cttcatcaac aggaagggtt
      121 ccgtttttcc gtaactcatg aagcgcacgg ttagccaggt atgcctggcg tgaggatccg
      181 ggataaacgc gggaggcggg cagcacatcg ctgttcagca ttgccggtac cttctgacag
      241 actgcatcag ccccatcgcc attacggtct ttttcatcaa atttattgac cagcacaaac
      301 aggcggccga ctgacacatc agcaatggca ttaagctctt tacgaacctc ctcatcggct
      361 ttcgagttca tctgggtgta gtccatcacc gccagcaccg cagaggcttt ctgcagttga
      421 tcccgcatca tcacttccat ctgcggttgt ccggcttcat tagggccggg ggtatccacc
      481 agtgtgagtg ttccctggca ttcatccatc cccaccagat ggctgaattc cacgtcgatc
      541 accggcagtt tctgcacttc tgcgtattca tcaaaaggaa attcactccc cattgccgca
      601 gcaagacgaa ccagatcgtt taacgatgcc agtccggtaa agattccctc ctccccgtgg
      661 tattcatttt tgagccagcc atcatccgtc agaatatcca gcagttcgcg ggtatccccg
      721 gtttgctgca gggtctgtgc gacctgctgt cctgccgggg tggcgacttt ttcctgcagt
      781 gtgattaata aattgcggac aggctgaata tgttccagat ggagaaccgg ctcagttttt
      841 ccgggaacgt ggcggatgag cgtcggtaca gaggtcatgg ggcggttacg gttaggcaga
      901 atttcctgcc ccacaatggc gttgatggtg gttgatttgc ctgctttcat cgtgccgaca
      961 acagccagca ccatctccct gcgggcggtt ttgatttgtt cgccctccag ctccttaatt
     1021 cttttcagtg ctttctgttt atcaaaaagc tgcccgtttt cgttcttacc gtcagataac
     1081 acatccggct catcaaccat ttgccggagc agattgatgc tcagttgcaa aagccggtcg
     1141 gcttcatcac aaagcagggt gatgttcttt tcgtgcatta tttaaccttc ctgagacgca
     1201 ttttcatgac aaccacggag gaggccattt actacaagcg atcgtttata acaatcaata
     1261 taatagaaac gatcgtcata ttctattaat atgaaaaaca caacgcaagg gggacaaccc
     1321 gggtatggct gcgtttgcat tgtgttttca tagagttaaa aatctgtaac gacaacccgg
     1381 cacatcacta ccgggttaag aagaaaatcc agttccatac cgcgcgggcc acagagacca
     1441 cggtgtcacg aacggcgcgc agggccgtac ggacaacaga ggcaacacag acactctccg
     1501 ccgtgtcaaa tatccggtcc accgcaccgg taaactgttc acgggtctga gtgcggacag
     1561 actccgtgcg cagttcgtcc tgcaggcgtg tcatcagagg gatggcggca tgtccgggaa
     1621 gcactgtcat gagtgcactg accggggtga cctccccctg aaacagtgca ttacagagtg
     1681 aggatttacc ggccccgctt ttacccataa tgccaggcac gggttcgtga cgggggagtt
     1741 tgcgcagatg ttccaggatg tgacgggaaa tcgggtaagg cagggaggag agcggttttt
     1801 caattgcctc aatggcatca gacggattca tacatgtatt cccagtgaaa aaagacaaaa
     1861 ccccgtgcac cggaaaggtg gcgggggatt cacggagata atatctttct gaaattattt
     1921 tgaatgcagg tatatgtggc tgatttgtat cagctaatga gtacaggtct gaatctaatc
     1981 tgacagtccg ctttgtgcca ggagcggact gacctgctcc ccgatgatta atacacagct
     2041 atgctagtaa tgtctgtaga tcgctgtgtg ttgcatctat cggttgaact cacagtccaa
     2101 accagactgt cagctttgat tagattctac ctactcaacc tgtcagataa ggtctgagct
     2161 aatacatcta atatatggct ttgtgcaggg gacggctatc gcgaacgtta ggctaggtat
     2221 ctgaatacgc acagttcact tggttgtact tcttgcactg atctgctatc ccgcacagct
     2281 atttataggt aatcttcagt gtataatctg ccgacttatg gaagcttgta caacaggaga
     2341 aatatatgaa aaaagaattg tttggacgtt gcccgtttga tctcgacgaa gatgtaaaaa
     2401 aaatagccgc gataaataaa tatatccatg caaaatttga ggagcataaa aataaaatag
     2461 acaacttaaa gggtgtggaa caaaaattga tggtcttaca gtactttaat gtactgaatg
     2521 aacttagtaa tcaggctatc tcttctttga atataggagc cttttcagca tcggaagtat
     2581 tagctagggt tataatagaa caggctgcca atcaatttta tatagcaata gatgatggcg
     2641 aaaatgcaca agcattactc aaggctagta agaaattagt tcatagtaat ggaaaacggt
     2701 ggctggagtg tttaaaatcc aaaggaatga ctaatccagc agcaaatgaa aggattcgca
     2761 tgggaaaaga actaactgat ctatttaata gattgtggcc aaatacacct gaatatccag
     2821 gaaccagaaa actttttgaa ttaattggct gggaaacaca ctatcatgcc cattacgttc
     2881 ccctttgtga ttcaatacat acattctctg atgatatggt aaatatagta tctctatata
     2941 atgcaatcca aagtgatgaa actactgcta tcgaattcat tcttgcagtt aaacaagaaa
     3001 ataaacggct ggcaatttat aattttgtaa ttgctacggc ccttcggtgt gaggccttag
     3061 taaatgtatt taactcttta ggttatcagg atattataac agaaatgaca ccaacaatag
     3121 atacagtcaa ccagactata attcgatatg atgattttga tcattccaga attgatgatt
     3181 gtcaataatt aattgacaga atacgtacat gcgcaacaac tagtgatagt tgacttattt
     3241 tcgctcttga gagacaacca tactcaaatc tcccacattg caggagattt gaatatgaaa
     3301 acgtcaccgt ggaataaaga ccgtaccatc ggtcaaaaaa ggccacttca gatatctcat
     3361 atctggggga tccggattag acttgagttg gaaggtaaaa cacgcgatct agctctgttc
     3421 aacatggctt tggacagtaa gctacggggc tgtgatttgg tcaaactcaa agtatcagat
     3481 gctgcatatg gcagttctgt ctcaagcaga gcaacggtgt tgcaacagaa aaccggcagc
     3541 cctgtccaat ttgagatgcc tttaataaca catcttttaa atgttgaaga attatcccgc
     3601 ctgctgaaga acgttgctct gagtgtgata aaccttttta ttcttgatga tgtcagcgag
     3661 aagatggaat atatccccga ctgggtcgtg gttacccggt taaacatctg gtggcgctaa
     3721 taaatctggc aagtcacatt ctgttgaatg ctgtgacagt gccttaccga gagagcgaaa
     3781 ttgtggtgtt ctagtgcata gtcgccacca ttccggataa ttctattgcg ttgttttaca
     3841 agttctttta cagcgcagat ctgtcgctga cgttgaatca atagcctgga tttcagaaca
     3901 caatgcccgt ttgctggttg gtccggcagt agcattaatt cattatctga atgaaatgtg
     3961 tcaatccaca gaaaccgctg tcaatgacgc attttgtcgg acgttaacac gagaaagtct
     4021 ggctattccg taatgccatc ttatttcgta atggattgag aatgctggat ttttaattat
     4081 attacgcgca gtattaatcg cgtaaatgtt ttgcggtcag caacggtggg ggatccattg
     4141 ctctgttcaa tgaataccat catacatttg aatcaggagt cggtatgatg attttggtga
     4201 cgggaggcgc atggagcggg aagagtcgcc atgcagaggt gcttattggg gactcttcac
     4261 aggttctgta tatcgctacc tcgcaaatcc ttgatgatga gatggctgca cggatagaac
     4321 atcatcggca aagccgcccg gagcactggc gcacagtgga gcgctggcaa catcttgatg
     4381 aattaattca tgcagacatt aacccgaatg aggctgtgtt gctggaatgc gttaccacaa
     4441 tggtgactaa tctgttgttt gattatggcg gcgataaaga ccctgatgaa tgggattatc
     4501 aggcgatgga acaggcgatt aatgctgaga ttcagtcgtt gattgctgcc tgccaacgtt
     4561 gccccgcaaa ggttgtatta gtgactaacg aagtgggaat ggggattgtg ccggagagtc
     4621 gtctggcacg acattttcgt gatattgccg ggcgggtaaa tcagcagttg gccgctgcgg
     4681 caaatgaagt atggctggtg gtttcgggta ttggagtaaa aatcaaatga gcaaattatt
     4741 ttgggcgatg ctctcattta ttacgcgtct gcctgtaccg cgtcgctggt cccagggact
     4801 ggatttcgag cattattctc gcggtattat tacttttcct ttgattggat tattgcttgg
     4861 cgcgattagc gggctggtct tcatggtgct gcaggcatgg tgtggcgcac cactggcggc
     4921 actgtttagc gtacttgtgc tggcgctgat gaccggggaa ttccatctgg acggcctcgc
     4981 tgatacctgt gatggcgtat tttctgcacg tagctgcgat cgcatgttgg aaatcatgcg
     5041 tgatagccgt ttaggcaccc acggcggtct ggcattgatt tttgtggtac tggcaaagat
     5101 tctggtgtta agcgagttgg ctctgcgtgg cgaaccgatc cttgcatcgc tggcggcagc
     5161 atgtgcggtc agccgcggga ctgccgcatt attgatgtat cgtcatcgtt atgcgcggga
     5221 agaagggctt ggcaatgtgt tcattggcaa aattgatggg cgacaaacct gcgtcacact
     5281 cggcttagcc gctatttttg ctgccgtatt attgctgggt atgcatggtg tggctgctat
     5341 ggtggtgacg atggtggcga ttttcatcct cggtcagctt ctcaaacgta cgctgggcgg
     5401 gcaaacgggc gatacgctgg gagcagccat agagcttggt gaactggtct tcctgctggc
     5461 actgctataa agagacatga catgcaaata attgccgatt tactgaatac gatcccagcc
     5521 atcaattctg ccgctatgtc gcgtgcacaa cggcatgttg acgggttact caaacctgtt
     5581 ggtagcctag gaaagctgga ggcgcttgcc atacaactgg caggaatgcc gggggtgaat
     5641 ggcataccgc atgtgggcaa aaaagcggta ctggttatgt gtgccgatca cggcgtctgg
     5701 gaggaagggg tcgctatttc tccaaaagaa gtgacagcca tacaggctga aaatatgacc
     5761 cgtggaacaa ccggcgtgtg tgtgctggca gcacaagcgg gcgctaacgt ccacgtaatt
     5821 gatgttggta ttgatactgc tgagcctatc cccgggctta tcaacatgcg tgtcgcacga
     5881 ggtagcggca atattgcttc agctccggca atgagtcgcc gtcaggctga aaagttgctt
     5941 ttggacgtca tatgttatac gcgggagctg gcgaaaaacg gtgtcacgct gtttggtgta
     6001 ggtgaactgg ggatggcaaa cacgacaccg gcagcggcaa tagtcagcac aatcactggc
     6061 cgggatcctg aagaagtggt tgggattggc gcaaacctgc cgacagataa actggctaat
     6121 aaaattgatg ttgtgcgtcg ggcgattacg ttgaatcaac caaatcctca ggatggtgtt
     6181 gatgtcctgg caaaagtggg tggatttgat ttggtcggaa tagctggagt gatgttaggt
     6241 gctgcttcct gcggtttacc cgtgttgctg gatggatttc tttcttatgc tgctgcgctc
     6301 gcagcctgcc agatgtctcc tgcaatcaaa ccgtatctca ttccttctca cttgtcggca
     6361 gaaaaaggcg cgcgtatagc gctctcgcat ttggggctgg agccttatct caatatggat
     6421 atgcgtttag gtgaggggag tggtgcagct ctggcgatgt ccatcatcga agctgcttgt
     6481 gcgatataca acaacatggg cgaacttgct gccagtaata ttgttctacc ggggaatacg
     6541 acttctgatt tgaacagcta aaaacgaaaa gtctgataaa agctatactt atcggatagt
     6601 attcgcgact taggaaaggg acatgatgcg tcgtgtaaat attctttgct catttgctct
     6661 gctttttgcc agccaaaata gcctggcggt aacttatcca ttacctccag agggtagccg
     6721 tttagtgggg cagtcgctta ctgtaactgt tcctgatcac aatactcagc cgctggagac
     6781 ttttgcctca caatacgggc aagggttaag taatatgctg gaagcgaatc cgggcgctga
     6841 tgttttttta ccgaagcctg gctcacaact caccattccg cagcaactga ttttgcccgc
     6901 cactgttcgt aaagggattg tggttaacgt cgctgaaatg cgtctttatt actatccgcc
     6961 agacagtaat acagtggaag tctttcctat tggtatcggc caggctgggc gagaaacccc
     7021 gcgtaactgg gtgactaccg ttgaacgtaa acaagaagca ccaacctgga cgccaacgcc
     7081 gaacacccgg cgcgaatatg cgaaacgagg ggagagtttg cccgcatttg ttcctgcggg
     7141 gcccgataac cccatggggc tgtacgcgat ttatattggc aggttgtatg ccatccacgg
     7201 taccaatgcc aattttggta ttgggctccg tgtaagtcag ggctgtattc gtctgcgtaa
     7261 tgacgatatc aaatatctgt ttgataatgt tcctgtaggc actcgtgtac aaattattga
     7321 tcagccagtg aaatacacaa cggaaccgga tggttcaaag tggctggaag ttcatgagcc
     7381 gctgtcgcgc aatcgtgcag aatatgagtc tgaccgaaaa gtgccattgc cggtaacccc
     7441 atctttgcgg gcgtttatca acgggcaaga agttgatgta aatcgcgcaa atgctgcgtt
     7501 gcaacatcga tcgggaatgc ctgtgcaaat tagttctggt tcaagacaga tgttttaaga
     7561 gcgttggtaa ttagaaagta aaaagcctgc tcgaaagcag gcttttttga atttggctcc
     7621 tctgactgga ctcgaaccag tgacatacgg attaacagtc cgccgttcta ccgactgaac
     7681 tacagaggaa tcgtgtgaac ggggcgcata ttaatggcag tgcatggtga tgtcaaaggg
     7741 tgaaatgcat ttttttgttt gtttgctgcc aatttcaaca aagtggtgag ctttcaatct
     7801 tattgggtaa tgaaccatcg tggtgcatac cctcctttta tagggcaggt gaatgcgact
     7861 gctgataaaa agggccaatg acgaatctat cataatctct tatttgacag ttggttagct
     7921 tgcgcatcaa cgccaaaata gaactggcag gcatcttgca tactggttgt aagtaatggc
     7981 ggcacttggg ccgattctta aaaaccggag gcaacatgaa cttcagacgc ctgaaatact
     8041 tcgtaaaaat tgtagatatt ggtagcctga cccaggctgc tgaagtattg catatcgcac
     8101 aaccagcgct cagccagcag gttgccatac tggaaggtga gttaaatcaa cagcttttga
     8161 ttcgcacaaa acggggcgtt acgccaacag acgccggaaa aattctctat acccatgcac
     8221 gggccatttt acgtcagtgt gagcaggccc aactggcggt gcataacgtt ggtcagtcat
     8281 tatcggggca agtctcgatt ggctttgcgc caggaaccgc tgcgtcatcc atcaccatgc
     8341 ccttattaca ggcggttcgc gctgaattcc cggaggtcgt tatctatctt catgaaaata
     8401 gtggcgcagt gcttaacgaa aaactgataa atcaccaact cgatatggcg gtgatttatg
     8461 agcattcccc tgtggctggg gtatccagtc aggctttgct gaaagaagat cttttcctgg
     8521 taggaactca agattgcccg gggcaaaacg ttgatgttaa tgctattgcg caaatgaacc
     8581 tttttctccc cagtgattac agcgctgtta gacttcgtgt tgatgaggct ttttccctac
     8641 ggcggctcac ggcaaaagtc attggtgaaa ttgagtctat tgccacgctt accgcagcga
     8701 ttgccagcgg catgggcgtt gcagtattac ctgaatcggc tgcgcgttcg ttatgtggcg
     8761 cagtaaatgg gtggatgtca cgcattacca ctccttcaat gagtctctct ttgtccttaa
     8821 atttacccgc cagagcaaac ttgtcaccac aggcacaggc agt
//
LOCUS       DADWTH010000139         8771 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.142, whole genome shotgun sequence.
ACCESSION   DADWTH010000139 DADWTH010000000
VERSION     DADWTH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.142"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JNY02_003610"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JNY02_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBB9434992.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="JNY02_003611"
     CDS             complement(984..1814)
                     /locus_tag="JNY02_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBB9434993.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2024..3037
                     /gene="intI1"
                     /locus_tag="JNY02_003612"
     CDS             2024..3037
                     /gene="intI1"
                     /locus_tag="JNY02_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBB9434994.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3240..3590
                     /locus_tag="JNY02_003613"
     CDS             3240..3590
                     /locus_tag="JNY02_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBB9434995.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3716..4276
                     /locus_tag="JNY02_003614"
     CDS             3716..4276
                     /locus_tag="JNY02_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB9434996.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4279..7245
                     /locus_tag="JNY02_003615"
     CDS             4279..7245
                     /locus_tag="JNY02_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBB9434997.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7312..7689
                     /locus_tag="JNY02_003616"
     CDS             7312..7689
                     /locus_tag="JNY02_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB9434998.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7890..8549)
                     /locus_tag="JNY02_003617"
     CDS             complement(7890..8549)
                     /locus_tag="JNY02_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBB9434999.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2480 c         2348 g         1993 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc ccaactttgt tttagggcga ctgccctgct gcgtaacatc
     1921 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1981 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     2101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg
     2161 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     2221 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     2281 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     2341 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     2401 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg
     2461 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat
     2521 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc
     2581 cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg
     2641 ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc
     2701 ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt
     2761 cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga
     2821 acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc
     2881 cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt
     2941 ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc
     3001 gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg
     3061 gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt
     3121 cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct
     3181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa
     3241 tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg
     3301 gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg
     3361 gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct
     3421 gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gccggaaggg gccgagtacg
     3481 tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga
     3541 ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct
     3601 aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat
     3661 tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac
     3721 tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact
     3781 ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg
     3841 tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag
     3901 catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca
     3961 acgcggcgtg catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc
     4021 gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat
     4081 ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag
     4141 gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga
     4201 gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt
     4261 gagaacggat cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc
     4321 ctgctggcgt tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc
     4381 gacctctcga tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag
     4441 ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc
     4501 ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc
     4561 cagcgggagc agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg
     4621 cccttcacca tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa
     4681 accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc
     4741 attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac
     4801 cggcgcatct acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac
     4861 gatctgctca agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct
     4921 ccggccaagc caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag
     4981 gcactcgatc tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt
     5041 gcccgcgagg gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc
     5101 tacgccactc tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc
     5161 gacctgcacg accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag
     5221 cagttccagg cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt
     5281 caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc
     5341 atgtcctggg attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat
     5401 gacttcgatt tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg
     5461 gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc
     5521 attgaggtgc tgcgcggcat gaacaccgac aacgcccgca agctgccagc cgatgcaccg
     5581 accggcttca tcaagccgcg ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg
     5641 cgctactacg aactgtgcgc gctgtccgag ttgaagaact ccctgcgctc gggcgacatc
     5701 tgggtgcagg gttcacgcca gttcaaggac ttcgaggact acctggtacc gcccgagaag
     5761 ttcaccagcc tcaagcagtc cagcgaattg ccgctggccg tggccaccga ctgcgaacaa
     5821 tatctgcatg agcggctgac gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg
     5881 gcagccaacg acctgccgga tgccatcatc accgagtcgg gcttgaagat cacgccgctg
     5941 gatgcggcgg tgcccgacac cgcgcaggcg ctgatagacc agacagccat ggtcctgccg
     6001 cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt ggacgggctt cacccggcac
     6061 ttcacgcact tgaaatcggg cgatctggcc aaggacaaga acctgttgtt gaccacgatc
     6121 ctggccgacg cgatcaacct gggcctgacc aagatggccg agtcctgccc cggcacgacc
     6181 tacgcgaagc tcgcttggct gcaagcctgg catacccgcg acgaaacgta ctcgacagcg
     6241 ttggctgaac tggtcaacgc tcagtttcgg catccctttg ccgggcactg gggcgatggc
     6301 accacatcat catcggacgg acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg
     6361 cacatcaacc caaaatatgg cagcagccca ggacggactt tctacaccca catctccgac
     6421 caatacgcgc cattccacac caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg
     6481 ctcgacggcc tgctgtacca cgaatccgac ctgcggatcg aggagcacta caccgacacg
     6541 gcgggcttca ccgatcacgt cttcgccctg atgcacctct tgggcttccg cttcgcgccg
     6601 cgcatccgcg acctgggcga caccaagctc tacatcccga agggcgatgc cgcctatgac
     6661 gcgctcaagc cgatgatcgg cggcacgctc aacatcaagc acgtccgcgc ccattgggac
     6721 gaaatcctgc ggctggccac ctcgatcaag cagggcacgg tgacggcctc gctgatgctc
     6781 aggaaactcg gcagctaccc gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc
     6841 cgcatcgagc gcacgctgtt catcctcgac tggctgcaaa gcgtcgagct acgccgccgc
     6901 gtgcatgccg ggctgaacaa gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc
     6961 aaccgccttg gtgaaatccg tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc
     7021 ctcaacctgg tgacggcggc catcgtgctg tggaacacgg tctacctgga gcgtgcggcg
     7081 catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc tattgcagta cctgtcgcca
     7141 ctcggctggg agcacatcaa cctgaccggt gattacctat ggcgcagcag cgccaagatc
     7201 ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg cttagcgtgc tttattttcc
     7261 gttttctgag acgaccccta ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat
     7321 tatctctgta aaaagttatt caaccggctt tccggtaccc tggtgatcag agcgcgctgc
     7381 ggcaataaca ttactggcct ggcatgttgc aatattcttt atccctcgcc ccgatacagc
     7441 ggtcagctgc atattaaaga gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt
     7501 aaagcgataa tgcgctttat agcccggctt gcgcttgaac aggaatgcct tagccttagc
     7561 tggaacgctg aaaaatccaa ccccggcgct aaccgttttt atcaggctct gggaggcaga
     7621 ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca
     7681 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     7741 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     7801 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
     7861 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     7921 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     7981 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     8041 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     8101 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     8161 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     8221 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     8281 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     8341 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     8401 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     8461 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     8521 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     8581 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     8641 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     8701 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     8761 caaagtgcgt c
//
LOCUS       DADWTH010000140         8592 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.143, whole genome shotgun sequence.
ACCESSION   DADWTH010000140 DADWTH010000000
VERSION     DADWTH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.143"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..323)
                     /locus_tag="JNY02_003618"
     CDS             complement(<1..323)
                     /locus_tag="JNY02_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB9435000.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(372..800)
                     /locus_tag="JNY02_003619"
                     /pseudo
     CDS             complement(372..800)
                     /locus_tag="JNY02_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(800..1507)
                     /locus_tag="JNY02_003620"
     CDS             complement(800..1507)
                     /locus_tag="JNY02_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB9435001.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1522..2199)
                     /gene="ydjY"
                     /locus_tag="JNY02_003621"
     CDS             complement(1522..2199)
                     /gene="ydjY"
                     /locus_tag="JNY02_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBB9435002.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2204..2914)
                     /locus_tag="JNY02_003622"
     CDS             complement(2204..2914)
                     /locus_tag="JNY02_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB9435003.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3081..3887)
                     /gene="xthA"
                     /locus_tag="JNY02_003623"
     CDS             complement(3081..3887)
                     /gene="xthA"
                     /locus_tag="JNY02_003623"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBB9435004.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4333..4911
                     /locus_tag="JNY02_003624"
     CDS             4333..4911
                     /locus_tag="JNY02_003624"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBB9435005.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            4927..5553
                     /locus_tag="JNY02_003625"
     CDS             4927..5553
                     /locus_tag="JNY02_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBB9435006.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5550..6383
                     /gene="astA"
                     /locus_tag="JNY02_003626"
                     /pseudo
     CDS             5550..6383
                     /gene="astA"
                     /locus_tag="JNY02_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
     gene            6379..7308
                     /locus_tag="JNY02_003627"
                     /pseudo
     CDS             6379..7308
                     /locus_tag="JNY02_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            7305..>8592
                     /gene="astB"
                     /locus_tag="JNY02_003628"
     CDS             7305..>8592
                     /gene="astB"
                     /locus_tag="JNY02_003628"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBB9435007.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2002 a         2208 c         2358 g         2024 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgtcaaaatg cgcagccagt cgggtacgca gggcatcatc gactgttggc
      421 ggtgtgacgc ttgcggcttc ggtgtactcc agcgccagcc gttcttcatc gctaaagagc
      481 gggctttgcc gccagtcagc cactgccagc agtttatcgg tgctgccggt acgctcggcg
      541 actttcatac tggtgatatc cacgcaaaac tcacacaggc acatttgcgc aatgcgcgcg
      601 ctgacaagcg atcgcactac cggatcgagc ggtgagcgtt tgcgctccag ccacccaaca
      661 aacattgaca ccaaataaaa gataaacggg atccgccccc accagcgaat cgggcttagc
      721 acctcgccgt aatggcgacg atgcagccac gcctgcggac gtaaaatgaa aggagttttg
      781 ctaagcggcg gtaaacccat cagtcattcc tcttctggcg ctcaagccat attttcttcg
      841 ccataaaaat caccacggtc agggcaaaca gaataaacag ttcggtgaca aaccagaaag
      901 taccgcctgt taacatgctg cccgcccagg aataaacaat agtcgccggt aattgaccaa
      961 gcccggtggc gataaaaaac gagcgaaaac gtattgaagt cagaccggca gcatagctga
     1021 ttggatcgaa agggacaaaa ggcaataacc gacagaccag gatggtgtgt ttgccgtagc
     1081 gagtgaaaaa gccgtccata ctgtcaagca cggttttgcc ggttaatttt tccaccactt
     1141 cgcggcccat cactctggcg ataaaaaagc acagcgccgc gccggccatc gaactggtcc
     1201 acgacagcaa gccgccccag aacgcgccaa acagcgacgc attggcaaag gtgatcaaaa
     1261 acgcaggtag cggtgcagca atggcctgta aaatcatcaa caagaacgag acaactgccg
     1321 ccagtgcgcc gtaagactga ataaagcgtt ctataccctg ttggtccacg gcggcaaacg
     1381 ccgcaacgct gcgattgata aactcatgga cgccgggaag cagcgcccat gcgagcagca
     1441 tagcgaacaa caggatgatg agggttatac ggtagtacca gattttacgc gattgtatct
     1501 tcatcatcct ttatcctggc tttattcggt gattttaaag gtaaccgttg ccagcgtgtt
     1561 atccgccggg agaactgagg cattgccttt gaatttcact tcgccgcgtt tttccaccgc
     1621 accataagtg tatgttgcat tgctgacgat gccgaccggg cagctatcca gacacaccag
     1681 gcaacccgtt ttcttctctt ctgctgccgt taaattaccg ccaaagcgca tgtccagttt
     1741 cttgccatta ctgtcaacaa tcacttcatc gaaggaatac gcttttgccg ccccttgcca
     1801 gttgaccgaa atatccagtt tgctgcctgt gacatgagtc gtttctttat tatccatcgt
     1861 catgttttcg cccggcgtac cacctgcctc tttcagggct tcataaaatg ctttcggggt
     1921 cgcataagcc ataaacagcg atttatgtcc gttggagcca tctttaaaca caataccgtg
     1981 acgagtgtcg tcggtgagga aacgaccatt aatttgcacc agcatggtta ccgtatggtt
     2041 attggcatcg acgcgcagcg gttggctgtt cgaaagtccg tcgtattcca cttttgccgc
     2101 cgcattctct ttttgatcgc agccgctaag cccggcaaca gccagtaagc agagtgcagc
     2161 cagtcctttt ttccatgaca ctgaataatg ttgcgacatc gttctatcct tcattttttg
     2221 ggtgagtaag tgggctgcgg cgcgaagcag acagatccac atgtttgtgt cgggcgtaga
     2281 gttttgcgag ctggatgaga ataaacagcg ccaggcccgc cagacagagt tgtaaaataa
     2341 agcgcagcgt aatgccttca ttggcgagat cgcttgccat cacggtataa ataacaatac
     2401 cgggcagggt cgtgagtgcc gaaataaggg tataaggcca gaaggcgatg gtggttaatc
     2461 cgtaagcgta attttgaata ttgtaaggaa acaacgggat taagcgggtc agaataagaa
     2521 aatcaatacc gttacgcgca atgccttttt caatggcctg gaaggtatgg ctatggccca
     2581 cgtatttcag cagtaaatca cgtcccagcc agcgcgccag caggaatgag cacgaggagg
     2641 ccagcgttgc ggcaattaat gagagtagtg tccctaagag cgggccaaaa actattcctc
     2701 cagcgatcac cagtatgctt cctggtaaca gaaagagggg ggcattgatg aataacagaa
     2761 tatagaggtt ataaccgaac aatccgctct ggcgaatgag tgtctgtaag tggggtaaat
     2821 cggtgagcag atcgaataaa ccgaaagcgt ggatagcgta aatgaccagc gcaaaaataa
     2881 gacaggcaaa cagaaagtta cgctcagcgt tcatcgtgga gtttcctata tcaaacttga
     2941 taatagagaa gtggtcagac aactaaaaac gcagtgacgg tataacaaag gacggcaggc
     3001 aacaaatcat tactgacagt ttgttgattt ttctcaaggt taattctcct gacccagttt
     3061 gagccaggag agctgctaaa ttagcggcgg aaggtcgccc agacgggggc gtgatcggac
     3121 ggtttttcca tgctgcgaat ttcatagtcg atgccggttt ctacgcaaca ttctgccagc
     3181 ggttggctgg cgagcagcag gtcgatgcgc agaccacggt tatcgtcaaa accttttgag
     3241 cggtaatcaa accatgagaa acgatctgct gtttgcggat tagcatggcg gaaggtatcg
     3301 accacccccc agctcatcag cctgtccatc cattcgcgct cttccggcag gaaagagcat
     3361 ttaccggtac gcagccagcg cttacggttt tcttcgccaa tgccgatatc cagatctgta
     3421 gggctgatat tcatatcgcc cataatcagt accggattat cacgtttgag ttcggtttcc
     3481 aggtagtttt gcagattctg ataaaactgc gcttttgccg ggaattttat cggatggtcg
     3541 cggctttcac cctgcgggaa gtaaccgttg atcacggtga cataacccag cagtgagggg
     3601 atttccgcca taataatccg ccgctgcgcc tcttcgtcgt caccgggaaa gccgcgacgc
     3661 acggcaatcg gcgtctcttt ggtcagcagc gccacgccat aatggccttt ctgcccgtga
     3721 taaaacacgt tgtagccgag cttcgccacc tcttcgagcg gaaacatatc gtcatgaact
     3781 tttgtctcct gcaggccaat cacatccggt tggtgctttt cgacgatggc ttcaagctgg
     3841 tgaggtctgg cgcgcaggcc gttgatatta aaagagacaa atttcatagt cgctgccatt
     3901 tattcagata aagagtgcgt ggatggtagc agaatttcgc gttgcttacc gcctgttgtt
     3961 aagtgatttg atcgatggtt gttaatgatg tcaacgatgg cgcaaaaaat gcccgctttt
     4021 ggtgcgcgct gcgtcagaat ggcgcagtaa tttccagtaa atttcgtcaa tgatcacaat
     4081 tccagaatta tattttactt ctgcataaca ttgcgttttt tattcttttt attcacccac
     4141 atcgcaaacg tattcacttt atatgcactt taaatgcata tggtttggtt ataactcctt
     4201 gatttccagt tagcctccgc cgtttatgca cttttatcac tggctgacac gaaccctgca
     4261 atctacattt acagcgcaaa cattacttat tgttaacata caaataacga attatttact
     4321 gtagaggtcg ctatgtctca gccaattacg cgtgaaaact ttgatgaatg gatgatacct
     4381 gtttacgctc cggcaccctt tataccggta cgtggcgaag gttcgcgctt gtgggatcag
     4441 caggggaaag agtatatcga cttcgcgggt agcattgcgg tgaacgcgct gggccatgcg
     4501 catccggaac tgcgtgaagc gctgaacgaa caggcgagta agttctggca taccggcaac
     4561 ggttacacca acgagccggt actgcgactg gcgaaaaaat tgatcgacgc cacgtttgcc
     4621 gatcgcgtct tcttttgtaa ctccggtgcg gaagccaacg aagcggcgct aaaactggcg
     4681 cgtaaattcg ctcacgaccg ctacggcagc cataagagtg gcatcgtggc gttcaaaaat
     4741 gcgtttcatg gtcgcacgct gtttactgtc agtgcgggtg ggcagccagc ctattcacag
     4801 gattttgcgc cactgccggc ggatattcgt catgctgcat ataacgatat taactctgcc
     4861 agcgcgctga ttgacgacgc cacctgtgcg gtgattgtcg agcccatcta gggcgaaggc
     4921 ggtgtggtgc cagccagcaa cgcgttttta caaggtctgc gtgaattgtg tgaccgccac
     4981 aatgccttgc tgatttttga tgaggtacaa accggcgtcg ggcgcactgg tgagctgtat
     5041 gcctatatgc actacggcgt gacgcccgat ctgttaacca ccgccaaagc gctgggcggc
     5101 ggtttcccgg tcggtgcgtt gttggcaacc gaagagtgcg cccgcgtgat gaccgttggc
     5161 actcatggca ccacctatgg cggtaatccg ctggcctcgg cggtagcagg caaagtgctg
     5221 gagctcatca acacaccaga gatgcttaat ggcgttaaac agcgtcacga ctggtttgtt
     5281 gagcgtctta atactattaa tcaccgctat ggtttgttca gtgaagttcg cggcttaggt
     5341 ttgttgattg gctgtgtact gaatgccgat tacgccgggc aagcgaaaca gatctctctg
     5401 gaagcggcga aagcaggcgt gatggtactg attgcgggtg gcaacgtggt gcgttttgcg
     5461 cctgcgctca atgtcagcga agaagaggtg acgaccggac tggatcgctt tgcagctgct
     5521 tgcgaacact ttgttagccg aggttcatca tgatggtcat ccgtcccgtt gagcgctcag
     5581 atgtctcggc gctgatgcag cttgccagca aaacgggcgg cggcctgacg tcgcttcccg
     5641 ccaatgaagc cacgctttcg gcgcgtatcg aaagggcaat caaaacctgg caaggcgaac
     5701 tgcctaaaag cgagcagggc tatgtgttcg tgctggaaga tagcgagaca ggcaccgtgg
     5761 cggggatttg cgctattgag gtggcggttg ggctgaacga tccctggtac aactatcgcg
     5821 tcggcacgtt ggttcacgcc tcaaaagagc tgaatgtcta taacgcattg ccgacgctgt
     5881 ttcttagtaa cgatcacacc ggcagcagcg agctgtgcac gctgtttctc gacccggact
     5941 ggcgcaaaga gggcaacggc tatttgctgt cgaaatcgcg ctttatgttt atggcggctt
     6001 ttcgcgacaa gtttaatgac aaagtggttg ctgaaatgcg cggggtgatt gacgaacacg
     6061 gctattcacc gttctggcaa agcctcggta aacgcttctt ttcgatggat tttagccgcg
     6121 ccgattttct ctgcggcacc gggcaaaagg catttattgc agaactgatg ccgaaacatc
     6181 cgatctatac ccacttttta tcccaggaag cccaggacgt catcggtcag gtacatccgc
     6241 aaaccgcgcc tgcccgcgcg gtgctggaga aagaaggttt tcgttaccgc aactatatcg
     6301 acatcttcga cggcgggcca acgcttgagt gtgacatcga ccgcgtgcgc gccatccgta
     6361 aaagtcggct ggtggaagtg gcagcaggct ggcttgccgc cgggcgtgct gaacctggtg
     6421 cagggcgggc gtgaaacggg tcaggcgctg agtgcgctgg aggatctcga cggtttgctg
     6481 tttaccggta gcgccaatac cggctaccag ctgcatcgcc agctctccgg tcagccggag
     6541 aaaattctcg cccttgagat gggcggtaat aatccgctaa ttatcgatga ggtggcggat
     6601 atcgacgcgg ctgtccatct gaccattcag tcggcgtttg tcacagccgg gcaacgctgc
     6661 acctgcgccc gccgtttatt gctcaaaagc ggagcgcagg gcgatgcgtt tcttgctcgt
     6721 ctggttgccg tcagccagcg attaacgccg ggcaactggg atgacgaacc gcagccgttt
     6781 attggcgggc tgatttctga acaggccgca cagcaggtgg ttactgcctg gcagcaactg
     6841 gaagcgatgg gcggacgaac cctgcttgcg ccgcgcttat tacaagcagg gacatcgttg
     6901 ctgacaccag ggatcattga aatgacgggc gttggcggtg tgccggatga agaggtgttc
     6961 gggccgcttt tgcgcgtctg gcgttatgat acttttgatg aggcgattcg gatggcgaac
     7021 aacactcgct tcggactctt ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa
     7081 ctgttgctgg aggcgcgggc ggggattgtt aactggaaca aaccgcttac tggtgctgcc
     7141 agtaccgcgc cattcggcgg cattggtgct tccggtaacc atcgccccag cgcctggtat
     7201 gccgcagatt actgcgcatg gcctatggcg agcctggagt cggactcgtt aacattgccc
     7261 gccacgctta accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag
     7321 ttaatttcga cgggctggta gggctgacgc atcattacgc gggcctgtcg tttggtaatg
     7381 aagcctctac ccgtcaccgt tttcaggtgt ctaacccgcg actggcggca aaacagggct
     7441 tactgaaaat gaaagccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg
     7501 agcgtccgtt tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac
     7561 tggaaaaagt tgcacgccag gcaccgcact ggctttccag cgtcagttcc gcttcgccaa
     7621 tgtgggtagc caatgcggca acgatcgcgc catctgccga tacgctggat ggcaaagtgc
     7681 atctcaccgt tgccaacctg aacaataaat ttcaccgttc gctggaagcg cccgtcactg
     7741 aatcgctgtt aaaagcgatt tttaaggacg aagagaaatt tagcgtccat tcggcgttgc
     7801 cgcaggtagc gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt
     7861 acggtgaacc gggtatgcta ctttttgtct acgggcgaga agaaggcaat gatacccggc
     7921 cttcccgtta tccggcgcga cagactcgcg aagccagcga ggcggtggca aggcttaatc
     7981 aggtgaatcc ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagagcg
     8041 tttttcataa tgacgtgatt gccgtgagta accgccaggt gctgttttgc caccaacagg
     8101 cgttcgctcg ccagtcacag ttactggcaa acctgcgggc gcgggtgaac ggttttatgg
     8161 cgatagaagt tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta
     8221 acagccaact gttgagccgc gatgatggtt ccatgatgtt ggtgctgcct caggagtgtc
     8281 gggaacacgc cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta
     8341 gcgaactaaa agtctttgac ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc
     8401 tgcggttgcg ggtggtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga
     8461 tgaacgatac cttgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgtc
     8521 ttactgctgc cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg
     8581 tattgagcca at
//
LOCUS       DADWTH010000141         8330 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.144, whole genome shotgun sequence.
ACCESSION   DADWTH010000141 DADWTH010000000
VERSION     DADWTH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.144"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..892)
                     /locus_tag="JNY02_003629"
     CDS             complement(<1..892)
                     /locus_tag="JNY02_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBB9435008.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGS"
     gene            952..1230
                     /locus_tag="JNY02_003630"
     CDS             952..1230
                     /locus_tag="JNY02_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435009.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="JNY02_003631"
     CDS             complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="JNY02_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBB9435010.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="JNY02_003632"
     CDS             complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="JNY02_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBB9435011.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3384..3716
                     /gene="hdeA"
                     /locus_tag="JNY02_003633"
     CDS             3384..3716
                     /gene="hdeA"
                     /locus_tag="JNY02_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBB9435012.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3832..4158
                     /gene="hdeB"
                     /locus_tag="JNY02_003634"
     CDS             3832..4158
                     /gene="hdeB"
                     /locus_tag="JNY02_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBB9435013.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4222..4868
                     /locus_tag="JNY02_003635"
                     /pseudo
     CDS             4222..4868
                     /locus_tag="JNY02_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4910..5440)
                     /locus_tag="JNY02_003636"
     CDS             complement(4910..5440)
                     /locus_tag="JNY02_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9435014.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5596..6162)
                     /gene="slp"
                     /locus_tag="JNY02_003637"
     CDS             complement(5596..6162)
                     /gene="slp"
                     /locus_tag="JNY02_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBB9435015.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6409..7632)
                     /locus_tag="JNY02_003638"
     CDS             complement(6409..7632)
                     /locus_tag="JNY02_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435016.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8213..>8330)
                     /locus_tag="JNY02_003639"
     CDS             complement(8213..>8330)
                     /locus_tag="JNY02_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBB9435017.1"
                     /translation="LTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2425 a         1614 c         1735 g         2556 t
ORIGIN      
        1 ggctaccttc atccactaat gccgtgacat acatgccagg cagcaagtca ccatttgggt
       61 tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc atcaaccgtc gggtcggaga
      121 atttcagcgt gccggtctgg ctgtaacgtt taccattttc cagattgagc tgtactggcg
      181 tactgccctg aacctgtttg atttgcccac tggcgacctc ttctttcatg cgtaagaaat
      241 cttgcaccga ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg
      301 aatctgcctg attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc
      361 ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg
      421 ctttggcgac ggtgacattg gcttctgctt cattcagctg ggtgcgcgcg gtgtcgtaat
      481 cctgacggct aacgtagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg
      541 cattgctggc ggtagagagc gctttcgcca gcgagccttt ggcggagttt agctcggcct
      601 gtaaaggtgc aggatcaatc tgatacagcg aatcgctctg gttcacttta tcgccttcga
      661 taaagttgcg tttaatgata ataccgccca cctggggacg tatctcggca gcttcataag
      721 gaacggttct accgggcaat tcgctcaaca cattgaccga actaggggag agtgtgacga
      781 caccgacctc aggcgtcatg gcggcggcgt tttccgccga tttgtcatcg caggcggtga
      841 gcatcgcgcc gcagaataac aacggtatta acagctttct tcttctgttc attttagtcc
      901 ctgaaaattc ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat
      961 ttacagacca gcggcatagt ggggttgtca gccagtaagg tgggatacag gcacagtgat
     1021 cgacatggtg aggtcaacga caaaaaaaat gttgccgttc tgccaacagt tcctgccagc
     1081 attcgggcaa cgaagggttc tactggtgga tacacatacc aggggaatca agtcgtttta
     1141 gcctctcctt tagtaatcac cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc
     1201 agccatcaat ttcagttgct tatgtcctga ctaaaaataa gatgtgatac ccagggtgac
     1261 gatgtcgctc atacgtctta gtttcagctt gtacataatg ttgaatttct gaatctttaa
     1321 tgtttgttgc gaaatattga gcgtcgacgt gatattgctt tcatttttat ttttcagaat
     1381 cagttgcata attttgaact gattatgtga aaaacataat tgtgaattct tatggggcaa
     1441 gtgtttacca taagtaatct caagaagatt tttgatactt tctttgcggc taacaaaata
     1501 aataggcgct ttagctttta gtttactgat gtgataaggt gccaaaacaa taattctctc
     1561 ggcatctaat ttctccagaa atttaatcgc ttcttcatca aggatatgat tgtgataggt
     1621 atcaatgata accttgaagg gtttattgcc tacttttttg atctctgaca gggaattgat
     1681 ttttatcatt tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt
     1741 cgtcatgaga aaaatcataa cttgctcctt agccgttatt gtttcgaaca tgtcatcctt
     1801 ttcattattt acatccttgt ccgaatcgtt gttcaatata gtaaacgcct ataagaactt
     1861 tgcccttcgt gaactaatac gtctattaac actaatcaat acttcagatt tatccatcac
     1921 gcactccata tattgatcct aagtaagatt cctggttgtt atcagcttgt aaatagtaaa
     1981 caaaaagtta acgtttgcgg attactgccc aagaataagt tatcaataac cagtttgtga
     2041 tctctgaaga atattactaa agttaaaatg taatccgatt taaatatcga gtctccttgt
     2101 ttcgacttaa gctggcaatt ggattgccag ctttctttta acgcattgaa attacctgag
     2161 taaataacgc atttatgtct tcaggttttt atatagcgtc tggagacacg gatatttatg
     2221 caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata
     2281 gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc
     2341 tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa
     2401 aaaaattaga tgaaattcag taggttgaaa taatcactag caggtaatta tttcaatgat
     2461 agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac
     2521 atcaagtgac tatgatccgg gtgacaaccg gggtaattat tgctgcttaa cgaacaaact
     2581 ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac
     2641 cagcgttaca gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag
     2701 cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat
     2761 caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat
     2821 gcccagctcc ggtgcacgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag
     2881 gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag
     2941 cgcaataccc gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac
     3001 gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat
     3061 tgctctgcga tgctttttca gcatctccag atcaaacttc aaaattgttg ccttatctat
     3121 atataacata gaaccaccct ataaaattaa gaagaaaatc ccctgctatc aatctatgcc
     3181 aaaaacgcgt ctaagaatgc agtcgattta ataaaaattt cctaattgca gtatctgatg
     3241 catctgtaac tcattgtatt gaaataaaaa tatctgattt tgatattttc catcaacatg
     3301 acatatacag aaaaccaggt tataacctca gtgtcgaaat tgattcgtga cggctctttc
     3361 actttatagt tgaggatatt acgatgaaaa aagtattagg cgttattctt ggtggtctgc
     3421 ttcttctgcc agttgtgagc aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac
     3481 cggtcaactc atggacctgt gaagatttcc tggctgtgga cgaatccttc cagccaactg
     3541 cagttggttt tgctgaagcg ctgaacaaca aagataaacc agaagatgcg gttttagatg
     3601 ttcagggtat tgcaaccgta accccagcta tcgttcaggc ttgtactcag gataaacaag
     3661 ccaactttaa agataaagtt aaaggcgaat gggacaaaat taagaaagat atgtaattcc
     3721 gggaatgcgt tacatcgtac ttccttgcat attgaacagg ccggaatatc ttctttaaaa
     3781 gcagctattc ctcctgttca tatataatct ctatattgaa tgggttacaa aatgaatatt
     3841 tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag cggctttgtc actggtgaac
     3901 gcacaatctg cgctggcagc caatgaatcc gctaaagata tgacctgcca ggaatttatt
     3961 gatctgaatc caaaagcaat gaccccggtt gcatggtgga tgctgcatga agaaacagta
     4021 tataaaggtg gcgataccgt tactttaaat gaaaccgatc tcactcaaat tcctaaagtg
     4081 atcgaatact gtaagaaaaa cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct
     4141 aatgacttgc cgaattaatg aggtacaagt aaaaaggagt agcaagttga gccatcttgc
     4201 tgctcctttt tgcattttta tatgacagca gaatttatta tacgtcttat actcgcggca
     4261 attgcctgtg gcgctattgg catggaaagg caaatgcgcg gcaaaggagc agggttacgc
     4321 acacatgtat taattggcat gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt
     4381 gctgacgtgc tgtctttaga tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg
     4441 gtgacgggcg tcgggtttat cggtgcaggt aacattctcg ttcgtaacca aaatattgta
     4501 ggtctgacga cggcagcgga tatctgggtg accgccgcat aggtatggtt attggcagcg
     4561 gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg gaagtcttcc
     4621 atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca ttagtgaacg
     4681 gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaact gatttagttt
     4741 cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt catgccacaa
     4801 cgttggtaga agacctgttg cgattattaa aaggtatggc aggggtgaaa ggcgtttcaa
     4861 ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt cacaccagat
     4921 aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg atcatcatga
     4981 tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc agaatatctt
     5041 cagttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc gaaagtgaca
     5101 cattcgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa tcaatgatag
     5161 tgatagtccg cggaacaaag gtcaccggta ctttccccag acagcgttta atattaaagg
     5221 gggagaaaac aatgacatgg acaggtttta atcgttcaat ttgagtgaga aaattagaat
     5281 gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc aacattacgt
     5341 cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg tttttcatcg
     5401 cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc tggtgcggac
     5461 attaacttta taatcagttt gccaaaggta attaacagga cagggcttat aataataata
     5521 gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag aaagcatctg
     5581 catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg cattggtgta
     5641 gtaaggcgca cccaagcccg gttccggcca gaatgcacca tagccgtaat cccacaggtt
     5701 ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca tattcacttc
     5761 caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa tggtaccgag
     5821 gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt gtctggcgag
     5881 cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca acggtaatac
     5941 agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct tcccaccaaa
     6001 gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa aacttttttg
     6061 aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg cggcaagcaa
     6121 aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat ccttatcaac
     6181 aaatattaaa accataatag atatttcttt atagttttca tctgattctg aggtgatatg
     6241 cctgatagta acgtaaacct gtctgatgtt tatatccctt ctgtttagtt caattccgtt
     6301 attttggggc gtggtatgag tatccatcgc ccaataatat aaccctttgt tttaaaatta
     6361 cataaaatct atttttattc aataattata aagtgctaat aatcaatatt atggtattat
     6421 attctgataa tgatttgata agcgaattct tttgccagat ggtatttcta tttccacccc
     6481 ataatttata tctttttcat taggcagatc tctctttaaa taaaaggggt atgaaatctt
     6541 ttcgggttga agtgtctcgt aatgaaatgc tatgaattca tttttagaca ttgttgccca
     6601 tttttgaatt ttattgtata gagagggtgt tgtaaactct tgaagttgtt tataacatat
     6661 ttttttgaac tcttgcttat attttaatat gtcatcaaat gaaagattca ctacctttgc
     6721 ctctaagaaa tcaattattc tcgaagcaat ttccgctttc gcgcgtaaag agttgtcatt
     6781 aatagttaac aatgtagaaa acaagccatt gatttgtttg tcaagatgtt gttcatattt
     6841 atgtagtttg gggatgggat tttccttgtg tgaaaaaata gtgttaaata aatttgcgtc
     6901 agtgaaattt atattaagta gatcttcaaa ataaaatttt gataaggtta ggactagctt
     6961 ggacatatct tgccctgtca tgttgaaatc aataaagtcg catccagcaa gggcttccat
     7021 cctattttca tttttactgt gctcaatata cagcttcgcc atttttaagt acaagctttg
     7081 aatgtctctt atatcggttg gtaagtcagc tcgttgccca gcaaaatttt tattatcgga
     7141 agaaacggtt tggacactga atctttcggg tattccttct tctacgagaa ttttagttat
     7201 gattggattg ttgggttctg gattaacgac aacaatgatt tgacatacag aatccttatc
     7261 cttttcgtat ctaatgttat gaatctgatt aagtattgat tgtgtctgtt gcgtaagtgg
     7321 tgtcgaactt atcgtttcaa tgttattaat aagacttaca agttgaacca caacttttga
     7381 gtagtctgca tttgtatatg tggtgttcgt gtggcatgca ttaatttgtt ggagttgttg
     7441 ttgcgctaaa tcaaattgtt cagggttgta tccttctcca gagaaggtgg aattaatggg
     7501 ttgttgttct tcccaattat cacaaggcca cttcggtgtg tctggcgttt taacattatc
     7561 ggtatttgtt gtgcagttat aaatatcgcc gtgatatgta tttattatac ctggggtaaa
     7621 gtcaattgac ataacattcc ttattcatca tctgttttat gctaatgttt ttaaattgga
     7681 taattagaac aggaatggat tttcttctac acacaataaa tataatgcgt tatatcttcc
     7741 tgatataatt acctttaaat tccctttcat ttccacccgg taatatttgt gtataaaaaa
     7801 agggcagtga tagtatcgta aaggaaaacg ttaacaacta tcaaaaagcg ctcattttaa
     7861 aaagttatta tgttctccga tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt
     7921 aataatgaac caggttaaag tgtatttaaa aatgtgttgt attagcgact gaaatctgcc
     7981 ttttaaaaag gaggagggta ctgagacaaa gagtcattgc ttctccgata aaatgtcaga
     8041 gaagcatgat tacgtggtta ttgtctgttg atgacttgtt gaccgagccg tcatcattct
     8101 caatgcatta gttaaatcat ttgtgatttt taaggattat cctgtctatg attttctact
     8161 tagaaggata tggcactaac tatgcatctt atagtttact gctggcatct atttaaaata
     8221 gcaattgagt caaatagccc atcaagatag ccatacctaa tataaccgtc aggaatgcag
     8281 ctatcatcgg cattctgaac attgatttaa gtaatatcac ttccgtcagg
//
LOCUS       DADWTH010000142         8320 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.145, whole genome shotgun sequence.
ACCESSION   DADWTH010000142 DADWTH010000000
VERSION     DADWTH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.145"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            334..3183
                     /locus_tag="JNY02_003640"
     CDS             334..3183
                     /locus_tag="JNY02_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBB9435018.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDIEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3283..5202)
                     /gene="dhaR"
                     /locus_tag="JNY02_003641"
     CDS             complement(3283..5202)
                     /gene="dhaR"
                     /locus_tag="JNY02_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBB9435019.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATMTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5430..6500
                     /gene="dhaK"
                     /locus_tag="JNY02_003642"
     CDS             5430..6500
                     /gene="dhaK"
                     /locus_tag="JNY02_003642"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBB9435020.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGR"
     gene            6511..7143
                     /gene="dhaL"
                     /locus_tag="JNY02_003643"
     CDS             6511..7143
                     /gene="dhaL"
                     /locus_tag="JNY02_003643"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBB9435021.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7154..>8320
                     /gene="dhaM"
                     /locus_tag="JNY02_003644"
     CDS             7154..>8320
                     /gene="dhaM"
                     /locus_tag="JNY02_003644"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBB9435022.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2070 c         2075 g         2001 t
ORIGIN      
        1 cccataacta attaggatta ttacaaaaaa tcttcaccaa attttttaac cgctgtttgt
       61 tgtaatattt ttgccatatt catagtaacg aattggaata agtaacgaca acttagggaa
      121 taactcgata taaactcacc tttgtaaggg gggtgttaaa tatatattaa ttcctgtgtc
      181 cctgattttg cccggtaata gagaattatt ctcagagaag ctcatcatga ttgcaccata
      241 cagatatcat gatgataaga ggatattatt cattgactta aacaaaatga caaaatccat
      301 agacccatag cctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat
      361 accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa
      421 tttggcgcta ttggtttgaa tgcaattatc ttttcccccc tgctgatagc tgctgataca
      481 ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac aggagatacc
      541 gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat
      601 atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga
      661 atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt
      721 ggtcaaacct ctgccatcgg cattaactta attggtgact atacccatgc tgacttaggc
      781 acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta
      841 acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat
      901 ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag tacaggtgtt
      961 tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac
     1021 ctgacaatcg atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt
     1081 gtcgatctcg gaacaaacag taccattaaa accaatggcg ataatgctca cggcctctgg
     1141 agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat
     1201 ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg
     1261 cagggcggtg gcctcgtcac cagtggttca gacgcgacaa tcaatttttc tggcacggca
     1321 gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg
     1381 gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag tctggcgctg
     1441 ggtttgtggg cgctcagcgg cggtagaata accggagaca gtttggctat caccggcgcg
     1501 gcaggagcca gagggattta tgccatgacc aacagccaga tcgacctcac gagcgatctg
     1561 gtcattgata tgagtacacc cgaccagatg gccatcgcaa cgcaacatga cgatggttat
     1621 gccaccagcc gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt tctttccaaa
     1681 ggtgggctaa tcaatctgga tatgcaccct gggtcggttt ggacaggttc ctccctcagc
     1741 gataatgtca atggcgggaa actggacgtt gcaatgaata acagcgtctg gaacgtaaca
     1801 agtaattcta atctcgacac gctggcgctg agccattcaa ctgtcgattt tgccagccac
     1861 gggtcaactg ccggcacatt taccacatta aacgtagaga acctgagcgg taacagtacc
     1921 tttattatgc gtgctgatgt tgttggcgag ggtaatggcg ttaataatag aggggattta
     1981 ttgaatatca gcgggagtag tgctggtaat cacgtattgg ctatccgcaa ccagggcagc
     2041 gaggccacaa cgggaaatga agttctgaca gtggtaaaaa ccactgacgg cgcggcctcg
     2101 ttcagcgcgt cttctcaggt tgagttgggg ggatatctgt acgatgtgcg taaaaatggc
     2161 actaactggg agctttacgc ttccgggaca gttccggaac cgactcctaa tcctgaaccc
     2221 acaccagctc ccgctcagcc tcccatagtc aaccccgatc ctacgcctaa acccaccacg
     2281 accgcagatg ctggcggcaa ttatctcaat gtcggttact tattgaacta tgttgaaaac
     2341 cgtacgctga tgcaacggat gggtgacttg cgaaatcaga gtaaagacgg taatatctgg
     2401 ttgcgcagtt atgggggaag cctggactcc tttgccagtg gcaaactgag cggctttgac
     2461 atgggttaca gcggtatcca gtttggtggg gataaacgtc tctctgatgt aatgccgttg
     2521 tatgtcggtc tgtatattga ctcaacacat gcatcgccgg actatagcgg aggcgacggt
     2581 accgcacgtt cagactacat gggaatgtac gccagttaca tggcacaaaa cggtttttac
     2641 agcgatctcg ttataaaagc atcgcgccag aaaaatagtt tccacgtact ggacagtcag
     2701 aacaactgcg ttaacgccaa cggcactgcg aatggaatga gcatctccct ggaagccggg
     2761 cagaggttca acctgtcccc tactggttat gggttctata tagagccgca aacccagctt
     2821 acatacagcc accagaatga gatggctatg aaggcgagta atggcctcaa tatacatctg
     2881 aatcactacg aatcgctgct ggggcgtgcc agcatgatac tggggtatga catcaccgca
     2941 ggcaacagcc agctgaatgt ctatgtgaag actggcgcta tccgcgagtt ttcaggggat
     3001 atcgaatatc tgttgaacga ctcccgggag aagtacagtt tcaaaggtaa tggctggaat
     3061 aacggcgtgg gagtcagtgc acagtataac aaacagcaca cattctatct cgaagcggat
     3121 tacacgcagg gtaacctctt tgatcagaag caagtcaacg gaggatatcg cttcagcttt
     3181 taagtgcggg atagacaagc ccttaaacga tgctgaaaat tgttagcgtg cgccggaatg
     3241 aaatacagat cactctgatg tatcagaaat cgtttctgtc tttcataccc ggcgcttaaa
     3301 ctgccctgca tcaatgccat gttgcttcat tttccgccac agcgtagtgc ggccgatccc
     3361 aagtaaagcc gacatttcct gaatgcgacc gcctgtgacc tgggctgcgt taataattgc
     3421 ctctttttca acttccgcaa atgacagact ggtggaaagg cgggtggcgc tgacatcatc
     3481 tgtcgcctgc tcggtaaaca gatgttcagg taaatcactg acgcgaatgc gcccgttatc
     3541 actactcaga gcaagattct cgatgacgct gtaaagttca aaatcgttgc ctggccatgc
     3601 acaagaaact aggcgagcga gggcatcgtc atcaattttc agccgcgtag agaagcgttt
     3661 ttcaagactg cgtaatttgt tattcaccag cgccggaatg ctgccacgcc gcatacgcag
     3721 aggcgggatg gtaatttcaa atgcatgcag cgcgtaatac agctggcgac taaaacgatt
     3781 ttgttccacc agcattgcga ggtccgcggt cattgttgca atcactttga catcaattgg
     3841 tattaaacgc cgcgcatcca gtcgcgtgat aaccccctgc ttgataacct gcagcaaagc
     3901 agactgtaac tccaccgcca gatattcaat cttttcaaga aacagcgtgc cgccgtgtgc
     3961 cagttccagc cgactcagac ggccattttc attgtccgtg cgatcgccac caataaattc
     4021 ttccgccagc gcagcatcac catataactc acaattgacg gcgatataag gacctgcagc
     4081 acgctcgctt tcattatgaa ttgcctgact tagcagtgcc ttgcccacgc cctcttctcc
     4141 acaaagcagg acaggaaagc tactgcgcgc cgcctggcga ccaaaatgaa tcaagcggcg
     4201 ggtttgcgga tcgtcctgtg gcatatgagc gaaggtatgg ctgacttttc ctaattgact
     4261 ggtcattaac tgccgcatct gttccacagg atggagcaac aaaataaagc tggttccctg
     4321 cgtttctatt atcggtttaa gggttatcac cgcatcaata aactggtgct ggctttcaaa
     4381 ggttgcttct acgtgtttga gcggatgtgc ctgttttatt gcttgttgca atacggcggg
     4441 taacgttaag agttcagtga ttgcccgtcc ctgacttgcc gtcgcgtcaa ggcgcaagac
     4501 ccgcgccgcc tgggcattaa taaattgcaa attaccctgc tcgtcccagc taatcacgcc
     4561 atcatccata ctttctaaca gggcattaag ttgatttaaa tgacggttag tttcagcgag
     4621 caaactgtcc gtcagcagta aattaccgac ctcgcgggcg attgccaacg tcaacggcaa
     4681 atcagctgcg gtagtttgct caaccggaca cgccagcgct attgttcccg tcaatcggcc
     4741 cttgctgtca aacaacggcg ttgcacaaaa ggcccagttc cagagtgcct gtttgaaatg
     4801 ttgatcggcc atcgttttca cggcctgacc agagatagcc gctaacgata gcgcacaagt
     4861 accaataatt ccctcggcgc aatacgtgcc gtcattgaat cccagtgcac ttagctgctg
     4921 caaggtttgc ggatcgccat tacggctgag aatgcaggcg gtttcatcga ggataaacag
     4981 cgcacactct cgcggtgcca tatattccca ggcatcttcc agcgcagcct gcccgagcgt
     5041 cagcatcgct ttcttacgcc gataaataga agcaaatgtc acgccctggg cctgatgtgg
     5101 tacgttccat gtctcccgtt tcatcagctt attgcatcgc tcccaggagg ttgcaattaa
     5161 gggagatatg ccccgaccat cgttgttaaa agcgccactc atatcccgca tcccttatga
     5221 caaaaggtgt tccggctcgc aattttaagt attaatacac gtaaaagttt caatatggaa
     5281 cattcattaa agcatatgtt tcactatgaa acataatttg ggctaatttt cttttccctt
     5341 gcctgatgca caatggattc atcggcagtc agtggtcgcc gtgtcgttga acatcatcca
     5401 tgccctaccg taattgctgg cgcaaaataa tgaaaaaatt gatcaatgat gtgcaagacg
     5461 tactggacga acaactggca ggactggcga aagcgcatcc atcgctgaca ctgcatcagg
     5521 atccggtgta tgtcacccga gctgatgccc ctgttgcagg aaaagtcgcc ctgctgtcgg
     5581 gtggcggcag cggacacgag ccgatgcact gtggttatat cggtcagggg atgctttcgg
     5641 gggcctgtcc gggcgaaatt ttcacctcac cgacgcccga taaaatcttt gaatgctcca
     5701 tgcaaattga tggcggcgaa ggtgtactgt tgattatcaa aaattacacc ggcgatattc
     5761 ttaactttga aacagcgacc gagttactgc acgatagcgg cgtaaaagtg accactgtgg
     5821 tcattgatga cgacgttgca gtaaaagaca gtctttatac cgccgggcga cgcggcgttg
     5881 ccaacaccgt attaattgaa aaactcgtag gcgcagcggc ggagcgtggc gactcactgg
     5941 acgcctgtgc ggaactgggg cgtaagttga ataatcaagg ccactcaata ggtatcgctc
     6001 tcggtgcctg taccgttcct gccgcgggca aaccttcttt tacccttgcg gataatgaga
     6061 tggagtttgg cgtcggcatt catggtgagc caggtattga ccgccgccct ttctcttccc
     6121 ttgatcaaac cgtcgatgaa atgttcgaca ccctgctgga acatggctca taccatcgca
     6181 ctttgcgttt ctgggattat caacaaggca gctggcagga agaaccacaa accaaacaac
     6241 cgctccagtc tggcgatcgg gtgattgcac tggttaacaa tcttggcgca actccgcttt
     6301 ctgagctgta cggcgtctat aaccgcctga ccacacgttg ccagcaagcg ggattgacta
     6361 tcgaacgtaa tttaattggc gcgtactgca cctcactgga tatgaccggt ttctcaatca
     6421 ccttactgaa agttgatgac gaaacgctgg ctctctggga cgccccggtc cacaccccgg
     6481 cccttaactg gggtagataa ggagaaagca atgtcactga gcagaactca aattgttaac
     6541 tggctcactc gttgtggcga tattttcaac accgagagcg agtatcttac cgggctggat
     6601 cgcgagattg gcgacgctga ccacgggcta aatatgaacc gaggctttag caaagtggtg
     6661 gaaaaactcc ctgctatcgc agataaagat atcggtttca ttctcaaaaa taccggtatg
     6721 acgctgcttt ccagcgtcgg tggtgccagt ggtccgctgt tcggtacctt ctttatccgc
     6781 gccgcacagg cgacccaggc acggcaaagc ctgacactgg aagagcttta tcagatgttc
     6841 cgcgatggcg cggacggcgt aatcagtcgc gggaaagccg aacctggcga taaaaccatg
     6901 tgtgatgtgt gggtgccggt ggtggaatcg ttacgtcagt ccagcgagca aaatctctct
     6961 gttccggtgg cgctcgaagc tgccagtagc atcgccgaat ccgcggcaca aagtacgatt
     7021 acgatgcaag cccgcaaagg ccgcgccagt tatctcggtg aacgcagtat tggtcaccag
     7081 gatcccggcg cgacctcggt gatgtttatg atgcaaatgt tggcgttagc cgcaaaagag
     7141 taaggaattg gtgatggtaa acctggtcat agtttcacat agcagccgac tgggagaagg
     7201 tgtcggtgaa ttagcccgtc agatgttaat gagtgatagt tgtaaaatcg ccattgccgc
     7261 gggaattgac gatccacaaa atcccattgg taccgatgcc gtcaaagtga tggaggccat
     7321 cgaatctgtt gctgatgccg accatgtgct ggtcatgatg gatatgggta gcgcattatt
     7381 gagtgctgaa actgcgctgg aattgctggc tcccgagatc gccgcaaaag tacgtttgtg
     7441 tgctgcgccg ttggtcgaag gtacactggc agcaacggtc agcgcggcct cgggggcgga
     7501 tatcgacaaa gttatctttg acgctatgca tgcgctggaa gccaaacgtg aacaactggg
     7561 tttaccgtcc tccgacactg aaatctctga cacatgtcct ccgtacgatg aagaagcccg
     7621 ttctctggcg gtggtcataa aaaaccgtaa cggcctgcat gtacgtccgg cctcccggct
     7681 ggtttatacc ttatcgacat ttaatgccga tatgttgctg gaaaaaaacg gcaaatgcgt
     7741 cacaccagag agtattaacc agattgcgtt actacaagtt cgctataacg atacgctgcg
     7801 cctgattgcg aaagggccag aagctgaaga ggcactgatc gctttccgtc agctggctga
     7861 agataacttt ggtgaaacgg aggaagtcgc tccacctact ctgcgtcccg ttctgcctgt
     7921 ttcgggtaaa gccttttatt atcaaccagt tttatgtacg gtacaggcaa aatcaaccct
     7981 gaccgcggaa gaagaacaag atcgattacg ccaggctatt gacttcacgt tattagatct
     8041 gatgacgtta acagcgaaag cagaagccag cgggcttgac gatattgccg caatcttttc
     8101 tggtcaccat acactgttag gtgatccgga actgctggcg gcggcaagcg aactccttca
     8161 gcatgaacat tgcacggcag aatatgcctg gcagcaagtt cttaaagaac ttagccagca
     8221 ataccagcaa ctggatgatg aatatctaca agctcgctat attgatgtgg acgatcttct
     8281 gcatcgcacc ctggtccacc tgacccaaac gaaagaagaa
//
LOCUS       DADWTH010000143         8201 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.146, whole genome shotgun sequence.
ACCESSION   DADWTH010000143 DADWTH010000000
VERSION     DADWTH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.146"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            59..460
                     /locus_tag="JNY02_003645"
     CDS             59..460
                     /locus_tag="JNY02_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBB9435023.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="JNY02_003646"
     CDS             570..1901
                     /locus_tag="JNY02_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBB9435024.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYTNL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /gene="narU"
                     /locus_tag="JNY02_003647"
                     /pseudo
     CDS             2225..3613
                     /gene="narU"
                     /locus_tag="JNY02_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3695..7328
                     /gene="narZ"
                     /locus_tag="JNY02_003648"
                     /pseudo
     CDS             3695..7328
                     /gene="narZ"
                     /locus_tag="JNY02_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7325..>8201
                     /gene="narH"
                     /locus_tag="JNY02_003649"
     CDS             7325..>8201
                     /gene="narH"
                     /locus_tag="JNY02_003649"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBB9435025.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKYIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         2190 a         2031 c         1912 g         2068 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacagaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatac aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat ataccccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 acttggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     7981 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     8041 caagtcagaa aaatatatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     8101 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     8161 catcgaggaa gcggcgagca ccgagcgcga agttgacctc t
//
LOCUS       DADWTH010000144         8194 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.147, whole genome shotgun sequence.
ACCESSION   DADWTH010000144 DADWTH010000000
VERSION     DADWTH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.147"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNY02_003650"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNY02_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBB9435026.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISVLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNY02_003651"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNY02_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBB9435027.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="JNY02_003652"
     CDS             complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="JNY02_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBB9435028.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPDFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="JNY02_003653"
     CDS             complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="JNY02_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBB9435029.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="JNY02_003654"
     CDS             complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="JNY02_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBB9435030.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3681..4508
                     /gene="nadE"
                     /locus_tag="JNY02_003655"
     CDS             3681..4508
                     /gene="nadE"
                     /locus_tag="JNY02_003655"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBB9435031.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4738..5625
                     /gene="cho"
                     /locus_tag="JNY02_003656"
     CDS             4738..5625
                     /gene="cho"
                     /locus_tag="JNY02_003656"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBB9435032.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5585..6160)
                     /gene="ves"
                     /locus_tag="JNY02_003657"
     CDS             complement(5585..6160)
                     /gene="ves"
                     /locus_tag="JNY02_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBB9435033.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLWLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6363..6848)
                     /gene="spy"
                     /locus_tag="JNY02_003658"
     CDS             complement(6363..6848)
                     /gene="spy"
                     /locus_tag="JNY02_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBB9435034.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7178..8146)
                     /gene="astE"
                     /locus_tag="JNY02_003659"
     CDS             complement(7178..8146)
                     /gene="astE"
                     /locus_tag="JNY02_003659"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBB9435035.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2153 a         2055 c         1890 g         2096 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtac gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcaatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagt
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgacccctcc ccaaagctta
     2281 gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc attaagcgaa
     2341 taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact gcaaaaggga
     2401 ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct taaaaaccgc
     2461 aatttaaata ttgcggtttt gatttatgaa ataactcttt gacgggaaaa tttaaaataa
     2521 attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg cctaaaccat
     2581 cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct
     2641 ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac
     2701 ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt
     2761 ctgcctgtgc gcgcattttt gataccagta aagagataga catgcccgca gaacaaaaca
     2821 gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca
     2881 ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt
     2941 tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata
     3001 aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg
     3061 aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact
     3121 caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag
     3181 gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct
     3241 gctttaccat cacgttgacc caggatgtag gtctggcaag taccgcgagc atggatcatg
     3301 ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt
     3361 ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata agctgtacaa
     3421 cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata
     3481 ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca
     3541 agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat
     3601 tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga agttttgtct
     3661 tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa
     3721 aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt cgattttctg aaaagctacc
     3781 tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca
     3841 cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgccaggaa actggcaacg
     3901 aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggctgac gaacaagatt
     3961 gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg
     4021 cggtattggc tagcgagcag gcattgcggg aagcaggcat tgaactgagc gattttgtcc
     4081 gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca
     4141 gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat
     4201 atggtgacgg cggtacggat attaatccgc tgtatcgtct caacaaacgc cagggtaaac
     4261 agttactgac ggcattaggt tgcccggaac acctttataa gaaagcccca actgccgatc
     4321 ttgaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata
     4381 tcgacgacta tctggaaggg aaaaacctac ctgaacaggt cgccagaaca atagagaact
     4441 ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa cgttttcgat gatttctgga
     4501 aaaagtaata atttgcacct attggattgt gcgaaaaaga gtaatttgtt cacgccggat
     4561 gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatacat tgcaggagtc
     4621 gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatca acccgcctct
     4681 tttttaatcg cctcccgcct gttacactgg atagataacc agcattcgga gtcaacagtg
     4741 gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc
     4801 gaacatttac attcattcct taatgactta cccacccgac caggggtgta tctgtttcat
     4861 ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg
     4921 ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc
     4981 tggatctgca ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa
     5041 caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta
     5101 aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac
     5161 ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagag catcgctgat
     5221 gaacaaaaac tttgttatgg cctgttggga ctggaaccgt taagtcgcgg tcgtgcatgt
     5281 tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa
     5341 catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa
     5401 ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac
     5461 tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc
     5521 gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat
     5581 gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca
     5641 gtttaccttg cggttgtaaa agccacaggg tgtgcctgcc atcgaaccag cacgcgccct
     5701 ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc gttaatcaca aacaccaccc
     5761 cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga
     5821 catccaggcg cgtcataata ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt
     5881 ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc
     5941 ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc
     6001 cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa
     6061 aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc
     6121 acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct
     6181 tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt
     6241 agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg
     6301 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa
     6361 gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt cagacgcttc
     6421 tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta aattttgttc
     6481 tgggtttcca tgtgcgccag catgttagct ttgcgctgtt cttccatttt tgcgatctgc
     6541 gcttcagctt ttgctttatc gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt
     6601 tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc gtggatctgc
     6661 tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg cggaccgaac
     6721 ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg
     6781 gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat
     6841 ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc
     6901 cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac
     6961 aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatgga
     7021 gagtgatgag agaatattac aacacgatga ttttgcagag attatgaaga actataccgg
     7081 atgactggtg ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc
     7141 aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt tcgagcatta
     7201 atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat
     7261 gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggca
     7321 taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg cgagtaattt
     7381 gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata
     7441 gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg
     7501 gcaacgcttt gccaagttcc aacgtacagg ccagcgcgcc aaaatgtctg acgctgaaat
     7561 gggtaaacgt accaccaggc tcctgatgga acaccagcgc ctccagcccc gccgcaccca
     7621 gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg
     7681 gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccaacgcaca gattctttgc
     7741 cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc
     7801 tttcagcaaa tagctgccaa cgaccgccaa acattcggtt catatcgcta tggcaataac
     7861 gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg
     7921 ggatctcgct gtgagatatc gcgccaagca acgcgtccag catttccaca ggtgccgtct
     7981 cattaccgtg gatccccgca gaaatcacca gcgcgccttg cggtggcgtt aatggcgtca
     8041 gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga
     8101 taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg
     8161 cgctggaacg gataaaccga accgagattc agta
//
LOCUS       DADWTH010000145         8158 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.148, whole genome shotgun sequence.
ACCESSION   DADWTH010000145 DADWTH010000000
VERSION     DADWTH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.148"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(61..600)
                     /locus_tag="JNY02_003660"
     CDS             complement(61..600)
                     /locus_tag="JNY02_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBB9435036.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /gene="fimA"
                     /locus_tag="JNY02_003661"
     CDS             complement(665..1213)
                     /gene="fimA"
                     /locus_tag="JNY02_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBB9435037.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNY02_003662"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNY02_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBB9435038.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="JNY02_003663"
                     /pseudo
     CDS             complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="JNY02_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4824..5540
                     /gene="nanC"
                     /locus_tag="JNY02_003664"
     CDS             4824..5540
                     /gene="nanC"
                     /locus_tag="JNY02_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBB9435039.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5560..6666
                     /gene="nanM"
                     /locus_tag="JNY02_003665"
     CDS             5560..6666
                     /gene="nanM"
                     /locus_tag="JNY02_003665"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBB9435040.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6731..7711
                     /locus_tag="JNY02_003666"
     CDS             6731..7711
                     /locus_tag="JNY02_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBB9435041.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTTLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2425 a         1743 c         1699 g         2291 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata agtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatgtata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg
     3301 ctttaaggag tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct
     3361 tattcctcat cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
     3421 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg
     3481 taagtggacc agttcttctg agcgaactta aatggagtag cgatagttac ttataataac
     3541 tttgccatac aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga
     3601 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
     3661 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttcaa
     3721 ctagccgtct caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca
     3781 tggaacgcag taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta
     3841 ttcctgcctg gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga
     3901 tatgggggaa cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc
     3961 actacgactt aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta
     4021 agtgaaacat catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag
     4081 tgaccagtta caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct
     4141 gatggcgagt aaacccttat tttcaccact ctcactgagt tagcttgcaa atctttcttt
     4201 gtttagattc taaataaatt atttataaaa catatagtta aaagaaaatc tttagttttt
     4261 gaagaccacc gcaagtgttc gtctggcttc acatggcatc ttcttcttag aaaaagatcg
     4321 acatattttg tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga
     4381 tctggtataa caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat
     4441 ctatggcaaa catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga
     4501 aaggaatatg aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt
     4561 gagtaagtga tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac
     4621 tcaggtattt aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg
     4681 agaccttata caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg
     4741 caaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
     4801 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
     4861 tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     4921 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     4981 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     5041 ccgcactcaa tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga
     5101 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     5161 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     5221 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     5281 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     5341 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     5401 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     5461 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     5521 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     5581 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     5641 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     5701 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     5761 gctgcattcc ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg
     5821 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     5881 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc gccgatgggc
     5941 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     6001 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     6061 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     6121 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     6181 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     6241 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     6301 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     6361 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     6421 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     6481 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     6541 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     6601 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     6661 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     6721 cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     6781 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     6841 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     6901 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     6961 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     7021 gcatattgca cggaaattac tgcccttcat tcctgataat gcagggattc ttatcgttcc
     7081 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     7141 cggagccagc catgatgctt gtcgttgggg aacggatact acgctgtacc aggatttagt
     7201 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     7261 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     7321 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     7381 tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     7441 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     7501 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     7561 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     7621 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     7681 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     7741 tgttcgttct cagagtagac aacaaaaaac aaatgagatc aaggtaatat ctcttcactt
     7801 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     7861 caccgcataa accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg
     7921 ccttttcgat tatggctcgg aaatctacga acatctacac agtttactgg aacgttttgg
     7981 gccacagcgt tcgcagcacc gtcctgatat gccgttctgg cgattataag tcgacggatt
     8041 ctggcaaata gacgatgatg atatgcttaa tgatgttcaa agaaattagg ccattattat
     8101 attattgctt gcattttctt tcattcctaa aagcacccgc cattaatccc aggcgcat
//
LOCUS       DADWTH010000146         8079 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.149, whole genome shotgun sequence.
ACCESSION   DADWTH010000146 DADWTH010000000
VERSION     DADWTH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.149"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1242)
                     /locus_tag="JNY02_003667"
     CDS             complement(<1..1242)
                     /locus_tag="JNY02_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBB9435042.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="JNY02_003668"
     CDS             complement(1239..2117)
                     /locus_tag="JNY02_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB9435043.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="JNY02_003669"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="JNY02_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="JNY02_003670"
     CDS             complement(3022..3255)
                     /locus_tag="JNY02_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435044.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="JNY02_003671"
     CDS             complement(3252..4109)
                     /locus_tag="JNY02_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBB9435045.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="JNY02_003672"
     CDS             4134..4463
                     /locus_tag="JNY02_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBB9435046.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="JNY02_003673"
     CDS             4453..4761
                     /locus_tag="JNY02_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9435047.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="JNY02_003674"
     CDS             complement(4803..4979)
                     /locus_tag="JNY02_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBB9435048.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="JNY02_003675"
     CDS             complement(4976..5386)
                     /locus_tag="JNY02_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBB9435049.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="JNY02_003676"
     CDS             complement(5383..5661)
                     /locus_tag="JNY02_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435050.1"
                     /translation="MNDSYRQFENWWSKDESQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..>8079)
                     /locus_tag="JNY02_003677"
     CDS             complement(5736..>8079)
                     /locus_tag="JNY02_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBB9435051.1"
                     /translation="GDCEPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPF
                     GGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1919 a         2203 c         1854 g         2103 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcatcgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcgga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctgcc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gctttcgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct
     6061 tttacgtcca ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg
     6121 agaatgacgt gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc
     6181 tgcccgttat agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac
     6241 tggatcccgt atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc
     6301 gttccagtca ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt
     6361 tccagcactg gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata
     6421 ccaggcttca gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt
     6481 gccatatgcc cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg
     6541 tggcgagggt gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc
     6601 ctgacgaata ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt
     6661 gaacggatgg cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc
     6721 ccatgccgct tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat
     6781 gacccgccga gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag
     6841 gaaaaaacaa gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc
     6901 atgcctatac gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt
     6961 gctgtgcacc ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca
     7021 cgcgccgttg atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca
     7081 gccttcatac gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta
     7141 agactaccat ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca
     7201 aagtcaccag ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac
     7261 cgcttcaacc cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta
     7321 gtgacctccc ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact
     7381 cggtaaacat ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct
     7441 actgacgcca cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct
     7501 aaaagaatag gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc
     7561 gtcaactctg cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac
     7621 agatactgct gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg
     7681 tagagttctt caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg
     7741 gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa
     7801 cgatgttcac cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg
     7861 tccatcgaga tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac
     7921 tgttgcttag cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc
     7981 gcatagctca tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc
     8041 agagcctgcc atccgaacag gcaaggttcg caatcacct
//
LOCUS       DADWTH010000147         8054 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.150, whole genome shotgun sequence.
ACCESSION   DADWTH010000147 DADWTH010000000
VERSION     DADWTH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.150"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(90..281)
                     /locus_tag="JNY02_003678"
                     /pseudo
     CDS             complement(90..281)
                     /locus_tag="JNY02_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            279..603
                     /locus_tag="JNY02_003679"
                     /pseudo
     CDS             279..603
                     /locus_tag="JNY02_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(633..3545)
                     /locus_tag="JNY02_003680"
     CDS             complement(633..3545)
                     /locus_tag="JNY02_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435052.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGAAPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3548..3664
                     /locus_tag="JNY02_003681"
     CDS             3548..3664
                     /locus_tag="JNY02_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBB9435053.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4028..4870
                     /locus_tag="JNY02_003682"
     CDS             4028..4870
                     /locus_tag="JNY02_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBB9435054.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGRECLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4873..5961
                     /gene="capU"
                     /locus_tag="JNY02_003683"
     CDS             4873..5961
                     /gene="capU"
                     /locus_tag="JNY02_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBB9435055.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5966..6916
                     /gene="virK"
                     /locus_tag="JNY02_003684"
     CDS             5966..6916
                     /gene="virK"
                     /locus_tag="JNY02_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBB9435056.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6981..7925
                     /gene="lpxM"
                     /locus_tag="JNY02_003685"
                     /gene_synonym="msbB"
     CDS             6981..7925
                     /gene="lpxM"
                     /locus_tag="JNY02_003685"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBB9435057.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2165 a         1810 c         1922 g         2157 t
ORIGIN      
        1 ggccttcccg agagtggtaa aggagaggcc ctggaaatac cccacagccc cgaaaaagag
       61 ccagtcagtt gcttaactga ctggcattaa ggccgccggg gacgtttttt taccaccggg
      121 agtttgggca taacggtgct ttcagtaagt acgacaggta ctgtggaggc acttccgtaa
      181 agaagggcat caagctcctg ctccgacata cctgcaggca aaggccatga aagccctgat
      241 ttccggaagc gagcgaacag tccacaaact gttgaacgtg gtgtcagcgg cggacaggga
      301 taagccctcg tcagcagttc cgtcagcact gcgacagcgt tctttacccg gtcaaaacag
      361 cgttgcggtg caccacgcct gacggatgaa ctgcgtgctc agggttacca ctttaacgta
      421 aaaaccgtgg cggcaagcct gcgccgtcag gggctgaggg caaaggcctc ccggaagttc
      481 agcccggtca gttaccgcgc acatggcctg cccgtgtcag aaaatctgct ggagcaggat
      541 ttttacgcca gtggtccgaa ccagaagtgg gcgggagaca tcacgtactt acgtacagat
      601 gaagttcggt tacatcctgt cagcacggaa cctcacgctt tttgagcggc acgacggatc
      661 ccccccaccc agtatccgac agacttttca ctgcgttcgt aaaaaccctt cttccctccc
      721 agcatatcca ctaattccgg tgacatatcc tgcagataag tctgcaaatc atgccccaga
      781 tcttccggtg actgcaactc ataaaccgcg cgccacacat caccctgcct tattttaagc
      841 cccgggttaa gaatatactg tccgtggttc agcctcagaa aaagcttttt tccataagga
      901 tttttgctgt taacttcatg ttgagagagc tggctgctga tataactcct tcgtttgcgg
      961 cgttccggca gcaatgcttc aggaagatga accacacact ctgccagtcg tgccgcactg
     1021 aaggcttccg aaaaaaacag agcacttccc agatgcgtgt ggaacagcgc ctgcatcagg
     1081 atcaccagaa acattgccat ctgccggttg tccagtttga tgaggcgtcc ttccacctgc
     1141 agtgacagac tttcaggttt cagcagggta tacaactgcg cacttttctg ttgcgcataa
     1201 cggggcgtac tgaccgccgc tgacagaact tgatgataag ccgccagccc gtcattaccg
     1261 gtcagcaagg gagctgcacc acgctccagc agtaactgaa ccagtgcaat attacctgcc
     1321 cgggctgctg acatcagcgg agtaagatta aacggcgaac ggtgatcaac accataacgt
     1381 tcaatatccc gcaacacggc aaccgggtta ttcattgagt atggccggaa atacttcagc
     1441 tccagtaact gcagagtctt cgttctgggc tgccttgtgg gcttaaaatc ttcccggtac
     1501 agtgcattat acagcgggaa cagggaataa atcagactgt actccagcgc ctgcagttgt
     1561 atggttttat cggctgtacc tgcgaggata tgctgtttcc actgtcgtgc atcctctgcg
     1621 gtgataatct gccatggcat ctcagatgtc tgcaggatcc ggctacggat ggtgtccgcc
     1681 tgctcctgct tcccctgttt ttcaagcaga tgtgcggttt tctgccactc atcacggctg
     1741 gaaatctctt cctgcagatt cagaggctct tcctgatgcg tgagcgcaag cagactccac
     1801 agaggatgct ccacctgctg ttcaacaaga tacacattgt gtgtagcacg ggtgagcgca
     1861 acatacagcg cgttggtaaa aaatttatag atttccgctg aacgatcctg cctgtcccgc
     1921 ggacgggaat aacgggcctc gccctcaaga tcagccggtg taagaccctc gcaaatatca
     1981 tcaaatgcct gccgggcagc gcttactata ttgtaaagga tcaccgtttc gtattccagc
     2041 cccttaacct cctgaaccga aaagaccagc ggcgtactga accagcatcg cgcctgtgct
     2101 ttctgttccg gatgcatcac aatgaccgct acacgattcg aaagtgaggt tttggcattc
     2161 agttcctgct ttgtctcttc cctgtcgtcc agcaaccgga tgctcccttc tgcctgaccg
     2221 cagcttctga caaaatggtg actttcccga tcaatagcac taaagcggac ctgtttgagt
     2281 ctcagcaacc ggttagccag tgcagtgacg tggtgcccgt tacggtaatt cgcctgcagg
     2341 atattaacgg ttgtgtcgtt tccctgttgc tgccggaaaa agagactttt gagcgaactc
     2401 catgagaaaa agctcggatg aactatctga ttggcatcac cacagagtaa aaactgaccg
     2461 ggatgacgta gcgttttcag taccagttgc agttgaacag gcgtcatatc ctgaacttca
     2521 tcaacaaaaa tgacatcata acggggagtt gccaacgaaa gatactgatg actgagcaga
     2581 ttactgtcat actgatgcga ctgtttcagc catgcgatat aacgttcaaa caggacatag
     2641 accgtcgggc ggtcctccat gccatagagc gactgccgta tccccagtga cagatacgcc
     2701 tcccgggaca gcggcccatt cccggaggca accgcaccga tcacgcccct gaactcctca
     2761 tacagggtat gggcagcccc cagtgcggcg atagccctgt tgcggggcag ccagtcgctg
     2821 aacgcactga aagtcacctc cctgccttcc ggaatgcgca gagtttccag aaattccgtc
     2881 agcgaaagaa agtcaatatt ctgtacctca ctcccttccc ctgaagcgtc atagagagta
     2941 cgcgcttttt ccaccagaaa tgaggaaagg ctgagataca gtatatcacc agcagcctgc
     3001 ttcatttttt ccagtaccag cgccgtctta ccacttcccg ccgagccaac aatcaccagc
     3061 ggtagcgggt gttcgtacag tgcctgctga tcatcatcaa aggacagcat tttatccagg
     3121 cggtggaaac gcccatgaga cggattgata tacgtcagcg cttcagtcgc gatatcaaca
     3181 ggatccggca ctggttgttg ttgtaagcgc ccttcatcaa tcaccacgtt tctgcgaaga
     3241 aaacgtgaag tgttataggc atgattgcga atatattcca gaacaaggca gatagtttca
     3301 ccgtggtagc gatacagcga aaacagtaat cggtcccgga tactgagccg ggcgcggtaa
     3361 agattggtgt caattttacg aacatcagcc tgaacaaagt tatcggtggc aatagcctgt
     3421 gcaaattttt tataacccgg gatcgtgtca ggattcagag tatgataaag acagattttc
     3481 atggctgccg gtttccgtcg tgcaaaaaac atgctgattt tacgaaggaa tgccttactg
     3541 aacaaccttg cccggaatac cccccgaaat cagatggaac aatcagaaaa atgctctgca
     3601 gttatggaat aaagaaggtg gggtggtacg ccgggagggc gatccgctgg aaatggcgca
     3661 gtagctgcag gcctgtcatg atgccggaat agaagttcgt gtacaggtta atgaaagcgt
     3721 caccccgtaa ataaactctg cgctgaaatt cctttttttc attggagcac ctgtgttgtt
     3781 ctgaggtgaa catatcacct ctgttcaggt agccaaattc agtgtgccac ctcaaggttc
     3841 ttagtgagtc gtttttaaaa gatatgataa tatatcattg tttatgaatt gttgatgttt
     3901 aatttagtaa attatatgaa atatgccatg ggtttgctat gttattcatc cagtatctgg
     3961 aaataaaatg caccagcttt attgggtttt ccttttgtct ctgcttacta ggtgccagaa
     4021 actttaaatg ttaaacgaag ggggcatttt gttcaaagcc aatcatgttc cggtattaat
     4081 gtatcatcat gtcagtcatt gccccggact ggttacactt tctcccgtga cattccggaa
     4141 gcaaataaaa tggttggctg aaaacaactg gaaaacactg tcatcagatg aactggagtt
     4201 tttttatcgg ggcggaaaac tcccccggaa aagcgttatg ttgacatttg atgatggata
     4261 ccttgacaac tggtttcagg tatatccttt attaaaagag tttaatctca aagcacatat
     4321 ttttttaatt accggtttta ttgggaatgg tccggttcgt cactctcctg gaaaggaata
     4381 ttctcatcgc gattgcgaac atcaaatagc gacagggaat gctgataatg ttatgcttcg
     4441 ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc gaatttcatg ttcatacgca
     4501 tactcataca cgctgggata aaaagttcag ttcacgagaa gaacagtgta agcatcttcg
     4561 gcaggacttg ttgagcggga gagaatgttt gaaagaaatg acaggaaaat gcagtaaaca
     4621 tttgtgttgg ccggaaggtt attataacaa ggattatatt caggtcgctg aagaacttgg
     4681 attctattat ttatatacaa cagaaagaag aatgaatgct cctgctaaag gaactacacg
     4741 gataggaaga ataagcacca aagaacggga aagttgcgca tggctgaaaa gacgactttt
     4801 ttattataca acaccgtttt tttcatcgtt gctggccttt cataaggggc caagattacc
     4861 ggatgattaa taatgaatat actatttacg gaatcatcac cgaatatagg tggtcaggaa
     4921 ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg ggcattcagt tctgcttgtc
     4981 tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat tgggaattga tatcacattc
     5041 gcgttatttc gaaacagtct tcacatccct actgcatgga gattactcgg aatagttcat
     5101 ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg atagcaatat tgttggttta
     5161 gtacggttat tcactaggaa acatccattc agaattatca ggcaaaagac atatttgaca
     5221 cgaaaaacaa aagttttttc aataaatcat ttttgcgatg aggtgattgt tcccggaaca
     5281 agtatgaaga cacatctgga gcaggaagga tgtcgaaccc gggttactgt tgtgcctcca
     5341 ggctttgatt tccagaaatt atacgttgat tcccgaaaca gtttgcctcc aaatgttctt
     5401 tcttggctgg cgtcccgaag gggatgcccc gttattgctc aggtaggaat gttgcgcccg
     5461 gaaaaagggc acgaatttat gttgaattta ctgttccatt taaaaatgaa tggacgacag
     5521 ttctgttggt tgattgtggg gtctggttcg cctgaactgc gggagcattt acagtatcag
     5581 attgacagta tgggcatgca tgatgatgtt tttattgctg acaatgtttt tcctgccgcc
     5641 cccgtatatc gggttgccag tctggtggtt ctgccttcag aaaacgaatc gtttggtatg
     5701 gtactggcag aagcatcggc attttctgtg cctgtactgg ccagtcagat tggtggaatc
     5761 cctgatgtta ttcagaacaa ccagaccggg acattgttac cagcaggtaa taagcacgca
     5821 tggatgtgcg ccctgaatga tttttttaat gaccctgggc gtttttatca gatggctcgc
     5881 caggcaaaac aggatataga agagcggttt gatattaata aaactgcgtt aaaaatactc
     5941 acattagcga agcacaagta acaatatgtt ttctgtaagt aacttatcat ttatcggttt
     6001 ccttaaaagg attgtttttt cctcagattc actcccgggg aagtgggaac acagaaaatt
     6061 ccggttcatg tacattttgc gatgtgctat aaatccggtt gccagtattc gatattatta
     6121 cgaactgcgt tccttgcagt gcattgagga tattctggca atacaaccca cgttgccagc
     6181 aagaattcat cgaccttatt tgcataaagg ggggcgagcc tggtcccggg ggcaatatat
     6241 tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa tattctgaat ttttgtttcc
     6301 acaaaaatcg gtatcattag tccaattcat cgggaaagat ggagaggatt ttgatatcca
     6361 gtgttctccc agtggttttg accgggaagg agagttgatg ttgtcattgt ttttcaatca
     6421 aatagtgatt gcccggttaa cattttcagt cattctgact caaaatggac acaccgcttt
     6481 tattggcgga ttacagggag cccccaaaaa taccggacct gatgttattc ggtgcgcaac
     6541 ccgggcctgt tatgggcttt tcccaaaacg gattattttt gaagcatttt gtgcgttgat
     6601 gaaagcttgt aatgtctctg agtgtctggc agtcagtgag cacagtcatg ttttccggca
     6661 attgagatac tggtatcaga aacgcaaaac ctttgttgct gtctacagtg atttctggga
     6721 gtctgtagca gggaaaacct gtggtgactg gtacaaatta ccaacacagg tggttcgaaa
     6781 gccgctaagc aatatagcta gtaaaaaacg ctctgagtac cgaaaacgat acgctttgct
     6841 ggattatatc catgaaactg ctatccgctc tttggatgca tatcctgtaa actcagaaca
     6901 tcaggactta aattaaggtt tgatgtattc tctgaataaa atattaatga tgattttggt
     6961 aggggcattc gcactaaata atgaaaaaat acaaatccga gtttattcct gaatttaaga
     7021 aaaattatct ttcccctgtt tactggttta catggttcgt tttgggaatg attgcaggta
     7081 tttcaatgtt tcccccttca ttcagagatc ctgtcttggc caaaataggg cgttgggtgg
     7141 gcagattgag cagaaaagct cgtcgcaggg cgacgattaa tttatcgctt tgtttcccgg
     7201 aaaagagtga tacagaacgg gaaataattg tcgacaatat gtttgccaca gcattgcaat
     7261 ccatagtgat gatggcagaa ctggcaattc gtggtccgga aaagttccag aaacgagtgt
     7321 tctggaaagg cctcgaaatt cttgaggaaa tccggcacaa taacagaaat gtgatttttc
     7381 tggttcccca tggctggagc gtggatattc ctgcaatgtt gctggcagct cagggggaaa
     7441 aaatggccgc catgtttcat cagcaacgaa atccagtgat tgattatgtc tggaattctg
     7501 tacggcgtaa attcgggggg cgcttacatt cccgggagga tgggataaaa ccatttattc
     7561 agtcagtacg ccagggatac tgggggtatt accttccaga tcaggatcat ggtcctgaat
     7621 acagtgaatt tgctgatttt tttgcgacct ataaagcgac attaccaatt attggacgtc
     7681 tgatgaacat cagtcaggct atgattatac cgcttttccc ggtttatgat gaaaaaaaac
     7741 atttcctgac tattgaagtt cggccaccaa tggatgcatg cattgccagc gcggacaata
     7801 aaatgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggtca catccggaac
     7861 agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc aaacgaagcg gacccgtacc
     7921 cttgaaatta taaagtacag gtatttccac tagttgtttc ttacaggtta ccaatcgaaa
     7981 cacatccccc ttccgtgtgt tgcccgtttg gcacggagga ctgtgtagat gcagttcgtt
     8041 aaatttaatt tatg
//
LOCUS       DADWTH010000148         7874 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.151, whole genome shotgun sequence.
ACCESSION   DADWTH010000148 DADWTH010000000
VERSION     DADWTH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.151"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..648
                     /locus_tag="JNY02_003686"
                     /pseudo
     CDS             1..648
                     /locus_tag="JNY02_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="JNY02_003687"
     CDS             645..1031
                     /locus_tag="JNY02_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435058.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGDYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LACWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3268
                     /gene="tssI"
                     /locus_tag="JNY02_003688"
                     /pseudo
     CDS             1056..3268
                     /gene="tssI"
                     /locus_tag="JNY02_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            3291..3735
                     /locus_tag="JNY02_003689"
                     /pseudo
     CDS             3291..3735
                     /locus_tag="JNY02_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3758..4165
                     /locus_tag="JNY02_003690"
     CDS             3758..4165
                     /locus_tag="JNY02_003690"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HBB9435059.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4162..6640
                     /locus_tag="JNY02_003691"
                     /pseudo
     CDS             4162..6640
                     /locus_tag="JNY02_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            7019..7375
                     /locus_tag="JNY02_003692"
                     /pseudo
     CDS             7019..7375
                     /locus_tag="JNY02_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            7595..7798
                     /locus_tag="JNY02_003693"
     CDS             7595..7798
                     /locus_tag="JNY02_003693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435060.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
BASE COUNT         2012 a         1821 c         2298 g         1743 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagt gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg acctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct tcgggaacga taccttaaaa acgcaactat aacgttgaat
      601 gtcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga ttattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctggcatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggcagcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcggc ggcgtcgctt gcagccggtg gtccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gggccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgcag gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg gcacatgggg ccggtcatat ccgtaccctc atgaccgggt acacctttac
     2281 gttgatgaat cacccgacgg cagaaattaa tcaggaatat ctgctggtac agaccactct
     2341 gtttttacgg gataatgcgc agcacagcgg tcaggatcag cattttacct atgtcaccac
     2401 gtttgaactg ccccccacca gcgaagttta ccgcccacaa cgaaccctga gcaaacccca
     2461 caccaaaggc ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa tctggacgga
     2521 taaatatggc cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga atgacgaaaa
     2581 cagttcctgc tggatccgcg tcagctatcc gtgggcgggt aaaggattcg gcatgatcca
     2641 gatcccgcgt attggccagg aagtactggt ggatttcaaa aacggcgatc cggatttgcc
     2701 catcatcgtg gggcgcacct ataaccagga caccatgccg ccgtgggggc tgccgggcat
     2761 ggcgtcgcag agtgggatat tcagccactc attgcaggga gggccgacta acggaaatat
     2821 gctgcgcttc gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg aaaaagacct
     2881 caataccacg gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca taaaaaccat
     2941 tgtcaaaaat gaaaccatca gcgtgggaga agaccgtaat gccaccatca caaaaaatga
     3001 tggtctttcc gtcaaactgg ctcaaaccat caatgtcgga acgacatatc gtctggatgt
     3061 tggcgatcaa tttacccttc gttgcggcaa tgcagcgctt gtattacaca aggatggctc
     3121 gattgagttt tgcggaaaac aactgttact gcacaccagc gatgttatgc aactcattgg
     3181 cagtggcatt gatatgaatc cggatggcgg cacagccatg actgcggatg atattgcccc
     3241 gttacctacc cccgacgttt ctgaataaaa aatataaaag gaatttttct atgaccagcc
     3301 tttaccgtat tcaggagggc tgctttgccc tgcctgaaac ttttcttgat cgcaccgtca
     3361 atatcttcgt cccatcgggc aatgaaaggg caactcccag tctgaatatt tcccgcgata
     3421 cgctcaggcc agatgaaagt ctgaccacct atattgaccg ccagatagcc ttgatgaaaa
     3481 aaaaacctgg gccagcaccg ggtactttcg cggggaacgg cactggcggg aactggcgaa
     3541 gctgccatga caggtgaaca gattctcgca actcatcgtt ctggaaaaac agaagtgtac
     3601 cagcgtcagg ccgggtttat caccgggcca gcaaaggtac taatgttgac gctgaccact
     3661 cagcgtcctt ttgatgatca caccgaccag ctatggactg catggttgac tagttttcag
     3721 cctgctaaaa gctaaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga
     3781 tgaccccatc taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat
     3841 cgccattatt gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat
     3901 tttgagtttt ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg
     3961 gcgctcgata cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa
     4021 cagtcgcccg gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga
     4081 aatccgcatt gcccagggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat
     4141 gaccacactg agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc
     4201 ggtacgcaga cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg
     4261 gatgtgctgt atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg
     4321 gcaaagatgg ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg
     4381 gtcgggatgg ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc
     4441 actgggcaaa aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc
     4501 acctgttcgc gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg
     4561 cggctgaact gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta
     4621 cagggtcggg aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac
     4681 ccggaagagc agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc
     4741 gaccgcagct attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt
     4801 atggagacgc cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg
     4861 gtggcctcca gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa
     4921 cgcctgtcgc atattgaact tgtgaagggc gggcgtcccg gtaacctggt ggaatatcgt
     4981 tacgacgata acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag
     5041 tttgcctatg aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc
     5101 taccactggg aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc
     5161 gaggattacc ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg
     5221 gagcagaagt ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg
     5281 ggcggcggaa tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc
     5341 cccggcgaac gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg
     5401 gacccggcag gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc
     5461 cgggcgctgg atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata
     5521 cgggagacgg acccggaagg gcgcgtcacg cggtacgggt atgatgaaca ggggctggcg
     5581 gtcagccgca cggatgcgcg gggcggcgag gttgtgctgg cgcacgatgc gcgcggtcaa
     5641 ctgaggcgtt acaccgactg ttcagggtgc gccacgggtt atgaatacga tgaagacggc
     5701 aacctgacgg cggtgacgga cgcggagggg aaaacggtac gcatcagcta taaccgtcag
     5761 gggctgccgg agacggtctg ccaccccggt aaacagcagg accgttacac ctggaacgcg
     5821 ctggggttac tgagcagcca ccgtcgcatc acgggcagtg tgcagtcctg gcagtatacg
     5881 ccgcgcggga tgctggcgcg ccatacggat gaagagaaac gggagacgcg ctggcagtac
     5941 accccggaag ggctggttgc cagtctgagc aacggcaacg gggctcagta ccggttcagc
     6001 tacgacggcg acggacggct gacgggagaa cagcgcccgg acggcctgat aaggatgttt
     6061 gcgctgaatg cggacgggtt tccggtcatc atccagacgc agggcacaga gggcggggtg
     6121 cgcaatgaac agcaggagcg tgatgcgctg ggccgcctgc tgcgcagtga cacgcagcac
     6181 agtacacgga ccttcagcta caaccgtctt gaccagataa cggaagtgac gctcaccccc
     6241 acggaagaag gggaacggct gcaccacatg caggcggaca cggtgcgttt tgcgtatgac
     6301 cgcagcgggt ggctgacggc agagcacagc gtgcacggca gtatcaaata ccggcgcgat
     6361 gcgctgggca atcccaccga tatcacgttg ccggacgggc agcatctcag tcatctttac
     6421 tacggcagcg ggcatctgct ccagacggca ctggacggta tcacggtcag tgaatatgag
     6481 cgtgacagcc tgcaccgtca ggtgatacgc acgcagggga aactggcgac gttcagcggc
     6541 tacaacgccg acaaccggtt aagctggcag cgcagcctgc caggtggcag ccagaatcca
     6601 cagcaggccg gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg
     6661 aacgcagacg gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc
     6721 tatgaccgca gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat
     6781 tattacgaca aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac
     6841 cgcctgccgg gctacgggcg tgaccgttac cgctataacg aatggggcga actgacagag
     6901 cgcagggacc agcaactgga gtggaacgcg cagggccagt taacgcgggt catcagcagc
     6961 aacagcgata cgctgtatca gtatgatgcg ctgggcaggc gcatcagcaa ggtgacatat
     7021 ggccgccatc acggggcgcg gagagagcca gaaaatctgg tattaccaca cggacctgac
     7081 gggcacggta cacgaggtaa ccgcgccgga cgggacgctg gtgtgggcgg gctaccaggc
     7141 gggatttggt gaaaaccggg gcgacatcag caacagcggg gcgtactttg agcagccgca
     7201 gcggctgccg gggcagtact ttgacgaaga aacagggctg cattacaatc tgttcaggta
     7261 ttatgcgccg gagtgcggga ggttcgtaag ccaggacccg ataggattaa acggtgggtt
     7321 gaacttatat gcttatgcgc ctaatccgtt aggttggatc gatccgctag gactgaattc
     7381 tttaggaata gattcaaata atatatttag aggagatggt aactataaag gtggcggaat
     7441 tggacagcca aagagtggca caatatcagc acaggacatt attgatcatc tctaagaaaa
     7501 aggtgatggg cgtttaacat cttttagtac taaaaaaaca tctagttcca actctcgtgg
     7561 tgcagatttt ttctcaaaaa accatgtctt caaagtggca gtttctgatc tcgatgattt
     7621 agttaaaaat aatcagatca ctgttttaac acaagaaaaa gtaaaagcaa tatttggttt
     7681 agaatcaagc aagaaaatta gaaaagaact tggtaatatt cttcagaata tggctaggaa
     7741 taatgaggtg ttagttcatg ggcaaattct tggaaataaa gttagccgat gtaattagtt
     7801 agtggagagg aagttgtgga cgaaattaga ataagaattg ctggtaatac ggctgcgcct
     7861 tgtttccatg tgct
//
LOCUS       DADWTH010000149         7819 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.152, whole genome shotgun sequence.
ACCESSION   DADWTH010000149 DADWTH010000000
VERSION     DADWTH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.152"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            211..429
                     /locus_tag="JNY02_003694"
     CDS             211..429
                     /locus_tag="JNY02_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBB9435061.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            647..889
                     /locus_tag="JNY02_003695"
     CDS             647..889
                     /locus_tag="JNY02_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435062.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVLGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="JNY02_003696"
     CDS             complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="JNY02_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBB9435063.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="JNY02_003697"
     CDS             complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="JNY02_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBB9435064.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2777..3679)
                     /gene="fdoH"
                     /locus_tag="JNY02_003698"
     CDS             complement(2777..3679)
                     /gene="fdoH"
                     /locus_tag="JNY02_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBB9435065.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="JNY02_003699"
     CDS             complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="JNY02_003699"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6155..6157),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBB9435066.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6936..7769
                     /gene="fdhD"
                     /locus_tag="JNY02_003700"
     CDS             6936..7769
                     /gene="fdhD"
                     /locus_tag="JNY02_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBB9435067.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1814 a         2013 c         2076 g         1916 t
ORIGIN      
        1 aatataatat attcatagca cccataactc atcctgggcg ataactaaac catttttgag
       61 taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg caaaaacaaa
      121 atgacatgca aaaatccgca tttttttaaa tgctttttcg gcgtaatttt cgcacataca
      181 ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta
      241 tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct
      301 gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc ggtgcgtaaa
      361 gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat
      421 aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat
      481 taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc gtatctggaa
      541 aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcggcaagg
      601 gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga actctctggc
      661 aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta agcaacttga
      721 tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag cggtagatca
      781 atacctgata aatcaatcgc aaacagcaag aaccagtgtt cttggcatct ggcaagggtg
      841 tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat gggaaaaaat
      901 gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg cgtgaacgcc
      961 ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt aggcctgata
     1021 agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata accttatcga
     1081 tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga atgggtaacg
     1141 cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg acctgcgcca
     1201 cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt taatggaact
     1261 acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga ggtcatctgc
     1321 cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc
     1381 atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt
     1441 gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg tttcacacag
     1501 gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg
     1561 catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc gggctttgcc
     1621 ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga taaacggcgc
     1681 tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca tatcttccag
     1741 ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc cgctcatttc
     1801 aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat cacgcggcag
     1861 aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc gcgcggtcag
     1921 atccatctcc agcggatggt cgtacagcac cacttcctgg gcgtgggcga taagcgcagc
     1981 aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac gctcggcgcg
     2041 gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat tcgccgtacg
     2101 tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc agctttcttt
     2161 tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg
     2221 gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc ggcgtagata
     2281 tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat taacgcgaat
     2341 cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat cacgccgctc
     2401 accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg accgaagtta
     2461 taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt cttcgcccaa
     2521 aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat gatgatgaac
     2581 gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg cggtgtgccc
     2641 atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct caccgccgcc
     2701 aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg
     2761 atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt
     2821 cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc gcaaaggtag
     2881 ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc acggtttcgc
     2941 tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca tggtgcagca
     3001 cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac
     3061 cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac
     3121 cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc ccaaccacca
     3181 cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg aggcgcggaa
     3241 tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg gactggaagt
     3301 cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct ttcaggcagc
     3361 ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt ttgtcgttct
     3421 gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca ttggggttgt
     3481 cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct gaacacgcca
     3541 cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc acttcttcct
     3601 ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga cgaatgatat
     3661 cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc acaaggaagg
     3721 acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa gtattggcaa
     3781 taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg atggtatcga
     3841 tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct ttggctttga
     3901 tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca agtttattcg
     3961 ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc aacagcgcgt
     4021 gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca tacgggaact
     4081 tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagcc gggttcgaga
     4141 taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg tagtgttccg
     4201 ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct tcctgctgca
     4261 tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga atatcccagc
     4321 cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac gggttaccct
     4381 gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat gcccatgccc
     4441 agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc atctggttgc
     4501 cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc
     4561 gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca ccggttgccg
     4621 gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct tccgacttcg
     4681 gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg ttcgcgccac
     4741 cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc tcaccgtcag
     4801 tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag cgacctgagt
     4861 taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac acttcggtct
     4921 ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag ttagaggttt
     4981 cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa ccgatcactt
     5041 tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag ccattgacct
     5101 tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc cacttcggca
     5161 accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag gccttcatca
     5221 tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc agcagcggtt
     5281 ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt ggcagcgtca
     5341 tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga atattggagt
     5401 gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc agcagctgga
     5461 tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag ccgagggcat
     5521 acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg cagactttca
     5581 ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg tagcgggaaa
     5641 cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg
     5701 cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg cgtttttccg
     5761 cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg atcaggctgg
     5821 cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc aggtacagca
     5881 atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag tagtcagcta
     5941 ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt
     6001 tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta ccgcccatca
     6061 ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa
     6121 atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg
     6181 cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg ttactcgacg
     6241 cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct tcggcgtttt
     6301 gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct
     6361 cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc ggaaacttca
     6421 gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa agtgcaccgc
     6481 ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg gcgttttttg
     6541 ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag gtgcaggtat
     6601 tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt gctacgctgg
     6661 gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag atcttaaaga
     6721 actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac gtcccattta
     6781 cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa ggggccgcaa
     6841 aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag aataccataa
     6901 tgttggtgtg tgtgttctta tctggttaag agaaagtgaa aaaaacacag cgaaaagaaa
     6961 tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg cgtgatgatt
     7021 tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg
     7081 gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt
     7141 tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc
     7201 cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt atggggttga
     7261 aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc gtggagcaac
     7321 ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt gatctcaaca
     7381 aactggatga tgcattacgt catctcaacg attttcagcc agtggggcaa ctgactggtt
     7441 gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg catgaagacg
     7501 tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa ggggaaagct
     7561 ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg caaaagtcgg
     7621 cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt gctgtagaag
     7681 tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg gcaacggttt
     7741 atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat atattaaatt
     7801 gcaggactta ttcatttcg
//
LOCUS       DADWTH010000150         7580 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.153, whole genome shotgun sequence.
ACCESSION   DADWTH010000150 DADWTH010000000
VERSION     DADWTH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.153"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..686
                     /locus_tag="JNY02_003701"
                     /pseudo
     CDS             1..686
                     /locus_tag="JNY02_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            686..1015
                     /locus_tag="JNY02_003702"
     CDS             686..1015
                     /locus_tag="JNY02_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435068.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            1015..1713
                     /locus_tag="JNY02_003703"
     CDS             1015..1713
                     /locus_tag="JNY02_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBB9435069.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1718..2461
                     /locus_tag="JNY02_003704"
     CDS             1718..2461
                     /locus_tag="JNY02_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435070.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            2398..2999
                     /locus_tag="JNY02_003705"
                     /pseudo
     CDS             2398..2999
                     /locus_tag="JNY02_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            3060..6539
                     /locus_tag="JNY02_003706"
                     /pseudo
     CDS             3060..6539
                     /locus_tag="JNY02_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            6607..7206
                     /locus_tag="JNY02_003707"
     CDS             6607..7206
                     /locus_tag="JNY02_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBB9435071.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            7270..>7580
                     /locus_tag="JNY02_003708"
     CDS             7270..>7580
                     /locus_tag="JNY02_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBB9435072.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     M"
BASE COUNT         1717 a         1807 c         2424 g         1632 t
ORIGIN      
        1 ggggagcctg aagcgacaac tgaatgaaaa caaacgctac tggcaggaac tggaagtggc
       61 gcagggcgac tggaaaaacg gtgccatgcg ggcgtttcag aattttacgg cggatgcgga
      121 taatgcggcg ggaacggcag aacagatgtt tacagtggca ttcagcagtg ccggtaatgc
      181 actggcgaca ttctgtacca ccggtaagct gaattttaaa tccttcacct cttccctgtt
      241 gtcagatatg gccagaatta tggcacagat ggccatgatg caggcggtaa agggcgtcgg
      301 ttctttattc ggcttcacga ctaatgctga tggcggtgtt taccagtctg ctgatttgag
      361 tcgctacagt ggcacggtgg ttaaccgtcc gacgtttttt gcttttgcaa aaggcgcggg
      421 tgtgatgggg gaagcgggac ctgaagccat tctgccactg cgtcgcggtg ctgatggtaa
      481 gctggggggt tgtggcggat actggtggtt caggcatggt gatgtttgcc ccgcagtaca
      541 acattgagat caataacgac ggcacgaacg ggcagatagg tccggctgcc ctgaaggtgg
      601 tttatgacct cgggaaaaaa gcggccgcgg actttatgca acagcaggcc cgtgatggtg
      661 gccggttaag tggagcatac cggtaatgga gacgtttcac tggaaagtgc gcccggatat
      721 gaatgtggta tcagagccga aagtggtgac agtgaagctg ggcgatggtt atgaacagcg
      781 tcgtgcggcg ggactgaata accagttgtc gacttacagc gtgacgatac gtgttcgtaa
      841 aggtgaacac ccatctttaa aagcctttct ggaacggcac ggtggcgtcc gcgcatttca
      901 gtggacgcca ccttatgact ggaaactgat cagggaggtt tgtcgtaaat ggtcggcaag
      961 cgtgggggcg ttatgggtga ctgtaacggc cgattttgaa caggtggtga actgatgcag
     1021 gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcagc ccgggtggag
     1081 ctctgggaaa tcgatctgac agaggtcggt ggtgagcgtt attttttctg taatgagcag
     1141 aacgaaaaag gtgagccggt cacctggcag gggcggcagt atcaggcata ccccattcag
     1201 ggggcggggt ttgaactgaa cggcaggggc tgtgctgccc gtccgacact gacggtctct
     1261 aacctgcacg gcatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg
     1321 gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac
     1381 agcgacgccg atccggaaca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa
     1441 ctgagcgcgg tcagtgcctc ttttgtgctg gccacaccaa cggagacgga tggcgcggtt
     1501 ttcccggggc gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc tgatgagtgt
     1561 ggttacacgg gcagggcagt ggctgacgag ttcgacaaac caacgacgga tatccggaag
     1621 gacaaatgca gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg taatttcggc
     1681 ggtttccttt ccatcaataa actttctcag taaatccatg acacagacag aatcagcgat
     1741 tctggcgcac gcccggcgat gtgcgccagc ggagtcgtgt ggattcgtgg tgagaacgcc
     1801 ggagggagac aggtatcttc ccagcgagaa tatctccggt gagccggagg aacggttccg
     1861 gatggctccg gaggactggc tgcgggcaca aatgcagggt gagattgtgg cactggtcca
     1921 cagtcatccc ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag
     1981 tgatttgccg tggtggctgg tctgccgggg ggcgattcac aagttccgct gtgtgccgca
     2041 tctcaccggg cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc
     2101 ttatcatctg gcggggattg agatgccgga ttttcaccgc ggggatgact ggtggcgtca
     2161 cggtcagaat ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc
     2221 atcagcacaa ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc
     2281 cgccatttac tgcggcgatg gtgagctgct gcaccatatt cctgaacaac tgagtaaacg
     2341 agagaggtat accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggaatg
     2401 gcacgcatct gcctttacgg ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg
     2461 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggcgtt tcgtcagaaa
     2521 ctgagtgacg gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga aaccgaattg
     2581 tctgcccgtc ttaatgagcc gctggcaaat ggtgcagtga tccacatagt accgcgtctg
     2641 gtgggagcta aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt
     2701 gctatatgga tgccggggta ggaattatgg cgagtaatct gctgttttct ctcggtgcca
     2761 gtatgacgct tggcggtgtt gcacagatgc tggcaccgaa agccagaact ccccgtacac
     2821 agacaacgga taacggcaaa cagaacacct gtttctcctc actggataac atggttgccc
     2881 agggcaatgt tctgcctgtt ctgtacggtg aaatgcgcgt ggggtcgcgg gtggtatctc
     2941 aggagatcag cacggcagac gaaggggatg gtggtcaggt tgtggtgatt ggtcgctgat
     3001 gcaaaatgtt tcatgtgaaa ccgcctgcgg gcggttttgt cgtttatgga gcgtgaggaa
     3061 tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc gaaggacaac ctgaaatcca
     3121 cgcagttgct gagtgtgatt gatgccatca gcgaagggcc ggttgacggt ccggtggatg
     3181 gattaaaaag cgtgctgctg aacggtacgc cggtcctgga cagcgagggg aagacaaact
     3241 tttccggtgt tacggtggtg ttccgcgccg gcgagcagga gcagacaccg ccggaggggt
     3301 ttgaatcttc cggctcagag actgtgctgg gtacggaagt gaaatacgac acgccgatca
     3361 cccggaccat cacgtcggca aacattgatc gtctgcgcct gaccttcggt gtgcaggcac
     3421 tggtggaaac cacctcaaag ggggaccgga atccgtcgga agtccgcctg ctggttcaga
     3481 tacagcgtaa cggtggctgg gtgacggaaa aagacatcac cattaagggc aaaaccactt
     3541 cgcagtatct ggcctcggtg gtggtgggta acctgccgcc gcgcccgttt aatatccgga
     3601 tgcgtaggat gacaccggac agcaccacag accagctgca gaacaaaacg ctctggtcgt
     3661 cgtacaccga aatcatcgat gtgaaacagt gctacccgaa cacggcgctg gtcggcgtgc
     3721 aggtggattc agagcagttc ggtaaccagc aggtgagtcg caattatcat cttcgcgggc
     3781 gcattctgca ggtgccgtcg aactataacc cgcagacgcg gcaatacagc ggtatctggg
     3841 acggaacgct taagccagca tacagcaaca acatggcctg gtgtctgtgg gacatgctca
     3901 ctcatccgcg ctaaggcatg gggaaacgtc gtggtgcggc agatgtggac aaatgggcgc
     3961 tgtatgtcat cggccagtac tgcgaccagt cagtgccgga cggatttggc ggcacggagc
     4021 cgcgcatcac ctgtaacgct tacctgacca cacagcgtaa ggcgtgggat gttctcagtg
     4081 atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg gcagacgctg acgttcgtgc
     4141 aggaccgacc gtcggataaa gtgtggacct ataaccgcag taatgtggtg atgccggatg
     4201 atggcgcgcc gttccgctac agcttcagcg ccctgaagga ccgccataat gtcgttgagg
     4261 tgaactggat tgatccggac aacggtcatg agacggcgac agagcttgtg gaggacacgc
     4321 aggccattgt ccgttacggt cgtaacgtca cgaagatgga tgcctttggc tgtaccagcc
     4381 ggggacaggc gcaccgcgcc gggctgtggc tgattaaaac ggaactgctg gaaacgcaga
     4441 ccgtggactt cagcgtcggc gcagaagggc ttcgccatgt accgggtgat gtcattgaaa
     4501 tctgcgatga tgattatgcc ggtatcagca ccggtggtcg cgtactggcg gtgaacagcc
     4561 agacccggac gctgacgctc gaccgtgaaa tcacgctgcc gtcctccggt accacgctga
     4621 taagcctggt tgacggaaat ggcaatccgg tcagcgtgga ggttcagtcc gtcaccgacg
     4681 gcgtgaaggt gaaagtgagc cgggttcctg acggcgttgc agaatacagc gtgtgggggc
     4741 tgaagctgcc gacgctgcgc cagcgcctgt tccgctgcgt gagtatccgt gagaacgacg
     4801 acggcacgta tgccatcacc gccgtgcagc atgtgccgga aaaagaggcc atcgtggata
     4861 acggggcaca ctttgacggt gaccggcgcg gcacggtgaa tggtgtcacg ccgccagcgg
     4921 tgcagcatct gaccgccgaa gtcaccgcag acagcgggga atatcaggtg ctggagcgat
     4981 gggacacgcc gaaggtggtg aagggtgtga gcttcctgct tcgcctgacc gtggcagcgg
     5041 acgacggcag tgagcggctg gtcagcacag ccaggacgac ggaaaccaca taccgcttca
     5101 ggcagctggc gctggggaat tacagtctga cagtccgggc ggtaaatgcc cgggggcagc
     5161 agggcgatcc ggcgtcggta tcgttccgga ttgcggcacc ggcagcgcct gtcactattg
     5221 aactgatacc ggggtatttt cagataacgg tggtcccgaa actggctgta tatgacccga
     5281 cggtgcagtt tgagttctgg ttttcggaaa agcggattgc ggatatcagg caggttgaaa
     5341 ccagcgcccg ctatcttggc acggcgctgt actggatagc cgccagtatc aatatcaggc
     5401 cgggccatga ttattatttt tacgttcgca gtgtgaacac cgttggcaaa tcggcatttg
     5461 tggaggccgt cggtcgggcg agcgattatg cggaaggtta tctgagtttt tataaagggt
     5521 tgatcaataa aacgcatctc ggcaaggagt tgtggacgca gattgataac ggtcagcttg
     5581 cgccggacct gactgaaatc aggacgtcca ttacgaatgt cagcaatgaa atcacgcaaa
     5641 ccgtcaataa aacactggaa aatcagagtg ctgcaatcca gcagatacag aaagttcagg
     5701 ttgatacaaa taataacctg aacagcatgt gggctgtgaa gctgcaacag atgaaggacg
     5761 gacgccttta tattgcgggt atcggtgccg gtattgagaa tacgccagca ggaatgcaga
     5821 gtcaggtgct gctggcggca gacaggattg cgatgattaa tcctgcgaat ggcaacacaa
     5881 agccgatgtt tgttggtcag agcgatcaga tattcatgaa cgacgtgttc ctgaaacgcc
     5941 tgacggctcc gaccattacc agcggcggta atcctccggc attttccctg acacctggcg
     6001 gacggctgac ggcgaaaaat gccgatatca gcggtaacgt gaacgcgaac tccgggacgc
     6061 tcaacaacgt cacgattaat aagaactgtc gggttctggg aaaattgtcc gcgaaccaga
     6121 ttgaaggcga tctcgttaaa acagtgggca aacctttccc acgggactcc cgggcaccgg
     6181 agaggtggcc atcagggacc attaccgtca gggtttatga cgatcagccg tttgatcggc
     6241 aaattgttat tcccgcggtg gcgtttcgcg gtgctaaaca tgagcggaag aataacaata
     6301 tttattcgtc atgccgcctg atagtgaaga aaaacggtgc tgaaatttat aaccgaacga
     6361 ccctggataa tacgctgata tatacgggtg ttattgatat gcctgccggt cacggtcaca
     6421 tgacgctgga gttttctgta tcggcatggc tggtaaatgg ctggtatccc acagcaagta
     6481 tcagcgattt gctggttgtt gtgatgaaga aagccactgc aggcatcacg attagctgaa
     6541 ttttataacc cagatacggg caccagaaat ggtgcctttt ttattgcaga aaagcgagag
     6601 gtaattatgc gtaaagtttg tgcagccatt ttgtccgcag ccatctgtct ggctgtatcc
     6661 ggtgcgcctg catgggcgtc tgaacatcag tccacgctga gcgccgggta tcttcagccc
     6721 catactgata tgccaggcag cgatgacctg aagggcatta acgtgaaata ccgttatgag
     6781 tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga agatgagcaa
     6841 aaaacgcatt acagcgatac ccgctggcat gaggattccg tgcgtaaccg ctggttcagc
     6901 gtgatggcgg ggccgtctgt gcgcgtgaat gaatggttca gcgcgtatgc gatggcgggc
     6961 gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac tgataacaag
     7021 gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa cacgtctctg
     7081 gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga ccttgcttat
     7141 gaaggttccg gcagtggcga ctggcgcact gacggtttca ttgtgggtgt cggttataag
     7201 ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg ggctttttgt
     7261 ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg taaacctgtc
     7321 gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt ggcacacgtt
     7381 gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga gccggggtat
     7441 tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga tattgatgtg
     7501 gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa ggatggtgat
     7561 ttacgtccgg aggtgatgaa
//
LOCUS       DADWTH010000151         7561 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.154, whole genome shotgun sequence.
ACCESSION   DADWTH010000151 DADWTH010000000
VERSION     DADWTH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.154"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1675
                     /gene="cpdB"
                     /locus_tag="JNY02_003709"
     CDS             <1..1675
                     /gene="cpdB"
                     /locus_tag="JNY02_003709"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBB9435073.1"
                     /translation="GLKAGDVHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGAK
                     FPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVVPPQIMGWDKANL
                     SGKVTMNDITETVRKYVPEMREKGADVIVVLAHSGLSADPYKVMAENSVYYLSEIPGV
                     NAIMFGHAHAVFPGKDFADIEGADITKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGK
                     WQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYSY
                     LALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPASYVEV
                     EKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIAPNSTKPQSLINWD
                     GFRTYNFDVIDGVNYQIDVTQPARYDGECQMINVNAERIKNLTFNGKPIDPNAMFLVA
                     TNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPI
                     AADKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(1801..2181)
                     /locus_tag="JNY02_003710"
     CDS             complement(1801..2181)
                     /locus_tag="JNY02_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB9435074.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            2270..3130
                     /gene="qorB"
                     /locus_tag="JNY02_003711"
     CDS             2270..3130
                     /gene="qorB"
                     /locus_tag="JNY02_003711"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBB9435075.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIARPTTTLAE
                     SVSHLFNVNN"
     gene            3239..4204
                     /locus_tag="JNY02_003712"
     CDS             3239..4204
                     /locus_tag="JNY02_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBB9435076.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLSQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            4312..4974
                     /gene="ytfE"
                     /locus_tag="JNY02_003713"
     CDS             4312..4974
                     /gene="ytfE"
                     /locus_tag="JNY02_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBB9435077.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5019..6431)
                     /gene="cycA"
                     /locus_tag="JNY02_003714"
                     /gene_synonym="dagA"
     CDS             complement(5019..6431)
                     /gene="cycA"
                     /locus_tag="JNY02_003714"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBB9435078.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(6739..7359)
                     /gene="fklB"
                     /locus_tag="JNY02_003715"
     CDS             complement(6739..7359)
                     /gene="fklB"
                     /locus_tag="JNY02_003715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB9435079.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         1942 a         1975 c         1928 g         1716 t
ORIGIN      
        1 aggattaaaa gcaggtgatg ttcatccggt ttataaggcg ctgaatacgc tggattatac
       61 ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg gattacctga aaaatgcgct
      121 ggcgggagcg aaattccctt atgtaaatgc caacgtcatt gacgccagaa ccaaacagcc
      181 aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc gataaagacg gaaaaaaaca
      241 gacgctgaag attggctata ttggcgtcgt gccgccgcaa atcatgggct gggataaagc
      301 taatttatcc ggaaaagtga cgatgaatga tattaccgaa accgtgcgca aatacgtgcc
      361 tgaaatgcgc gagaaaggtg ccgatgtcat tgtcgttctg gcgcattccg ggctgtctgc
      421 cgatccgtat aaagtaatgg cggaaaactc agtttattac ctcagtgaaa ttccgggcgt
      481 taacgccatt atgtttggcc atgctcacgc cgttttcccg ggtaaagatt ttgctgatat
      541 cgaaggggct gatatcacca aaggcacgct gaatggtgtt ccggcggtaa tgccgggcat
      601 gtggggcgat catcttgggg tggtcgactt acaactcagt aatgacagcg gtaaatggca
      661 ggtgacgcag gcgaaagcgg aagcacggcc gatttacgac atcgccaata aaaaatccct
      721 cgcggcggaa gacagcaagc tggtagaaac actcaaagcc gatcacgatg ccacacgcca
      781 gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg tatagctatc tggcgctggt
      841 gcaggacgat ccgaccgtgc aagtcgtgaa caacgcgcaa aaagcgtatg tcgaacatta
      901 cattcagggc gatccggatc tggcaaaact gccggtgctt tcagctgccg caccgtttaa
      961 agttggtggt cgcaaaaatg acccggcaag ctatgtggag gtggaaaaag gccagttgac
     1021 cttccgtaat gccgccgatc tttatctcta ccccaatacg ctgattgtag tgaaagccag
     1081 cggtaaagag gtgaaagagt ggctggagtg ctccgccgga cagtttaacc agattgctcc
     1141 taacagcacg aaaccacagt cactcatcaa ctgggatggt ttccgcactt ataactttga
     1201 tgttattgat ggtgtgaatt atcagattga tgttacccaa cccgcccgct atgacggcga
     1261 gtgccagatg attaatgtca atgcagagcg gattaagaac ctgaccttta atggcaagcc
     1321 gattgatccg aacgctatgt tcctggttgc caccaataac tatcgcgctt acggcggcaa
     1381 atttgccggt acgggcgaca gccatatcgc ttttgcttca ccggatgaga accgctcggt
     1441 gctggcagcg tggattgctg atgagtcgaa acgtgcgggg gaaattcatc cggcggcaga
     1501 taacaactgg cgtttagcac cgatagctgc cgataagaaa ctggatatcc gtttcgaaac
     1561 ttccccgtca gataaagccg cagcgtttat taaagagaaa gggcagtatc cgatgaataa
     1621 agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg gatttgagta agtaaaacac
     1681 ttctttttcg gcctataaat caaccgcatc cggcatttat tggcggatgc gatgctgccg
     1741 tatcttatcc accctacaag ccatgaaccg tagaccggat aagcgcagcg catccggcag
     1801 ttatgccgca cgttcatccc gcaccgccaa cacctcgggc aaattcaact caatccagtc
     1861 cgccagtgca gcaacctttt cgctcacctg ctcgcccagc ggcgtgaggc tatattccac
     1921 atgcggcggc accaccggat acgcgatacg gttaagaaaa ccatcctgtt ctaacgcctg
     1981 taacgactgc gcaagcatct tttcactcac cccaccgatt ttgcgccgca ggtcgctaaa
     2041 gcgatgagta ccttcgcgta gcgccaccag aatcaacacc ccccaacggc tggtgacgtg
     2101 tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga ttaccctctt tcagttgttg
     2161 tgacaggcta acctgactca tttcatactt acctttttgc atgtacttac taaaagtaag
     2221 tttaggtgtt agcgtattta aacacaagac aaaacgatgg agacttccca tgatcgctat
     2281 tactggtgcc actggccaac ttggtcacta tattattgaa tccttgatga aaacggttcc
     2341 tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa gcccaggccc tggcagcaca
     2401 aggcattacc gtgcgtcagg ctgactacag cgatgaagcc gcactgacat ctgcacttca
     2461 gggagtggaa aaactactgc tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca
     2521 tcgtaatgtt attaatgccg caaaggcggc tggcgtgaaa tttatcgctt ataccagcct
     2581 gctacatgca gatacctccc cgctcggcct cgccgatgag cacatcgaga cggagaaaat
     2641 gctggctgat tctggcatcg tttacaccct gctgcgcaac ggctggtaca gcgaaaacta
     2701 cctcgccagc gccccggcag cactggaaca cggcgtattt atcggtgcgg cgggcgatgg
     2761 caaaatcgcc tcagcaacgc gggcagatta tgcgacagct gcggcactcg tgattagcga
     2821 agccggtcac gaaggcaagg tttacgaact ggcgggcgat agtgcctgga cgttgacaca
     2881 gttagcggca gagctgacca aacagagcgg caaacaggtt acctatcaaa atctgagcga
     2941 agccgatttc gccgcggcac tgaaaagcgt cggactgccc gacggactgg cggatatgct
     3001 ggcggattct gacgttggcg catcgaaagg tggtctgttt gataacagca aaacgcttag
     3061 caaattgatt gcccgcccaa cgacaacgtt agccgaaagc gtaagccatc tttttaatgt
     3121 taataactag ttaattaaag tggcatcctc ccgcatcctc tctgataatg acgggatgcc
     3181 gggagcaatc atgtctgctt cctgaacgtt tcttctgaca gaccaatgga tgccagtaat
     3241 gattagcggc gtgctgtacg ccctgttagc agggttgatg tgggggctta tttttgtcgg
     3301 gccgttgatc gtgccggaat acccggcgat gttacagtcg atggggcgtt atctggcgtt
     3361 agggttaatt gcgctaccca ttgcctggct gggacgcgtg cgtctgcgtc agttggcgcg
     3421 tcgggactgg cttaccgcct tgatgctcac tatgatgggc aacctcattt attacttctg
     3481 ccttgccagt gccattcaac gtactggcgc gcctgtttcc acgatgatta tcggcaccct
     3541 gccggtggtg attcctgtct ttgccaatct gctttatagc cagcgcgacg gcaaactcgc
     3601 gtggggaaaa ctcgccccgg cactgatttg tattggcatc ggcctggcga gtgtgaatat
     3661 tgctgagtta aaccacggac tccccgattt tgactgggca cgttatacct ctggcatcgt
     3721 gctagcgtta gtttccgtgg tctgctgggc atggtatgcc ctgcgcaacg cccgctggct
     3781 gcgggaaaat cccgacaaac atccgatgat gtgggcgacg gcgcaggcgc tggtcacgct
     3841 gccggtttct ctcatcggct atctcgtcgc ctgttactgg ctgaatatac aaacgccgga
     3901 cttctcctta ccttttggcc cccgtccgct ggtgtttatt agtctgatgg ttgcgatagc
     3961 cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc gccagccagc gattaccgac
     4021 agtgattctc gggccgctga ttgttttcga aacgctggca ggtttgctgt acaccttttt
     4081 actcagccag caaatgccgc cgctaatgac gctgagcggt atcgcgctgt tagtgattgg
     4141 cgtggtcatc gcggtcagag caaaaccaga aaaaccttta actgaatctg tctcagaaag
     4201 ttgacatgct ggcagtgagt taaataagcc tctgctacgt aagggttata gcttttgcct
     4261 taaaggtgta tttaaaatac atcttatctt attaagaatg aggtatcagc tatggcttat
     4321 cgcgaccaac ctttaggtga actggcgctc tctatccctc gcgcttcggc tctgtttcgt
     4381 aaatatgata tggattactg ctgtggcggt aagcagacgc tggcgcgcgc ggcggcacgt
     4441 aaagaactgg atgttgaggt cattgaagct gaactggcaa agcttgctga acaaccgatt
     4501 gagaaagact ggcgtagcgc cccgctggca gaaatcattg accatatcat cgtgcgctac
     4561 cacgatcgtc accgcgagca gctgccggag ctgatcctgc aagcgactaa agtcgagcgc
     4621 gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa aatacctgac catgctgcat
     4681 gaagagcttt ccagccacat gatgaaagaa gagcagatcc tcttcccgat gatcaaacaa
     4741 ggcatgggca gccaggcaat ggggccaatc agcgtaatgg aaagcgagca cgatgaagcg
     4801 ggcgaactgc tggaagtgat caaacacact accaataacg tcacaccgcc gccagaagcc
     4861 tgcaccacct ggaaagcgat gtataacggc attaatgaac tgattgatga cctgatggat
     4921 cacatcagtc tggaaaacaa tgtactgttc ccacgcgccc tagcgggtga gtgatctaaa
     4981 agctggatgg cattgcgcca tccagcatga taatgcggtt atttccgcag ttcagcagcc
     5041 cgcttcttac caataaacag ccagcccaac cccagcgcga taaaccacag cggggtaacc
     5101 agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca cgaccacgaa cacaaagaac
     5161 gccatacata cccagcacat cagcttgccg agcggcatct tgtagatcga cttttcatgc
     5221 agatgaggac gctgtttgcg gtacaccagg tacgagcaaa ggataatcgt ccagacgaac
     5281 ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg cgccaatcac gctaggattc
     5341 acatacaaca tcaccacgcc gccgagcaga cagatacacg agaaggtcaa ccctttcgcg
     5401 ggtactgcgc gcttagaaag tttagcgaac gctttcggcg caacaccttc ctgcgccaga
     5461 ccaaacagca tacggctggt agagaagaca ccgctgttag cggaagacgc cgcagaggtc
     5521 agcaccacaa agttgatcac gctggcagcc gcaggcaaac ctaccaacac aaacagctca
     5581 acgaacgggc ttttctccgg gactaccgaa ctccacggtg tcacggacat aatcacaatc
     5641 agcgcgaaga cgtagaacat aatgatacgg atcggaatgg agttaatcgc gcgtggcagt
     5701 gatttctccg gatctttggt ttccgcagct gttgtaccta ccagctcaat ccccacgaaa
     5761 gcgaaaaccg ctatctggaa tccggcaaag aagccactta aacctttcgg gaaccagccg
     5821 ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg tcggtgactg aaagtgcatc
     5881 gccaccatga ccaggccgac gacaatcagc gacacgatgg cgacgatttt gatcatcgca
     5941 aaccagaact ccatctcacc gaacattttc acggtggcaa ggttgagcgt cagcagcagc
     6001 actatcaccg ccagcgaggc gacccagtcg gagaaatcgg ggaaccagaa ctgagcataa
     6061 gccgtgatcg ccaccacgtc tgccataccg gttacaaccc agcagaacca gtaagtccag
     6121 ccggtgaaat atcccgccca cggccccagt aaatcggaag cgaagtcact aaaagatttg
     6181 tattccagat tcgaaagcag caattccccc attgcccgca tcacgaaaaa gagcataaaa
     6241 ccaatgatca tataaacgaa aatgatcgac ggcccggcaa ggctaatcgt tttgccggac
     6301 cccataaaca acccagtacc aatggcaccg ccaatggcaa taagctgaat atgtcggttt
     6361 gtgagattgc gccgtagcga ctgttcagcc ggagcctgat catcggcaac gacttttacc
     6421 tgatctacca tgttttttct tcctgtacct gtctgtgttg ttcaggctct acggccttta
     6481 gtcagtctat gatacgacga atctgtatcg ggactcatcg atattaggta acaatacgcg
     6541 ggatgaatac taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca
     6601 cggtgataac gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa
     6661 tagcgccgtt tggcaaactg aagggtttat tgctgaatgc ctgctcccct ctggttcgag
     6721 gggaacagaa tgcgctgctt agaggatttc cagcagttcg acttcaaaca ccagggtgct
     6781 gaacggaggg atggatgcac ctgcgccgcg ctcgccatat gccagttcct gcgggatagt
     6841 cagttcccat ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac
     6901 gccattaacc gggaattcag cgggttcacc acgagcaacg gagctgtcaa acacggtgcc
     6961 gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc
     7021 ttcaccctgg ttgatcacgc ggaattgcag gccagattcg gtgctattca caccttcttt
     7081 tttggcgttt tcttccaggt atttcacacc ttcagcagcc atcgcctgga aacgctgacg
     7141 acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg cgatgcacca catcaaccgg
     7201 cacagccgga tgtttgcctt ccagcgcatc ggcaatacct gcaaccagtg cttctggcag
     7261 cagcccttcc aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct
     7321 tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta
     7381 taaaagtagc gggcagcata tcagccacac ttctttgggt aaaactttgt cgcaggaatg
     7441 atgacaaatc ccggggaaaa agaaacaata gcaatatccc gtatgaatat aataaaggcg
     7501 ggttggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa ggtgcttgcg
     7561 t
//
LOCUS       DADWTH010000152         7513 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.155, whole genome shotgun sequence.
ACCESSION   DADWTH010000152 DADWTH010000000
VERSION     DADWTH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.155"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNY02_003716"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNY02_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBB9435080.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="JNY02_003717"
     CDS             962..1303
                     /locus_tag="JNY02_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBB9435081.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="JNY02_003718"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="JNY02_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="JNY02_003719"
     CDS             complement(1901..2611)
                     /locus_tag="JNY02_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBB9435082.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="JNY02_003720"
     CDS             2719..3024
                     /locus_tag="JNY02_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBB9435083.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..3546
                     /locus_tag="JNY02_003721"
                     /pseudo
     CDS             3223..3546
                     /locus_tag="JNY02_003721"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4102..4701
                     /locus_tag="JNY02_003722"
                     /pseudo
     CDS             4102..4701
                     /locus_tag="JNY02_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5294..6283)
                     /locus_tag="JNY02_003723"
     CDS             complement(5294..6283)
                     /locus_tag="JNY02_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435084.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6358..6867)
                     /locus_tag="JNY02_003724"
     CDS             complement(6358..6867)
                     /locus_tag="JNY02_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435085.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QLIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(6969..7190)
                     /locus_tag="JNY02_003725"
     CDS             complement(6969..7190)
                     /locus_tag="JNY02_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435086.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRHLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
BASE COUNT         1891 a         1898 c         1871 g         1853 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agttgtatat attcagtact cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gacccccggt ggcctctttg tcaggactca
     4801 gggtggcacc ggggaacatt ggtgtcttaa ttgtattgaa tgaggtctct aacttttggt
     4861 ccaaaactca tcgttagctg ctcgccagct cacagccgaa cccgaactgt aagcacgacc
     4921 accagcacca gcttcagcag gaagacgaca agatagactt tatgcaggtc catgcgccct
     4981 cctgtgtcta ttgtaccact gcactagata actagtacaa aataaagtcc agaccgaagg
     5041 cccagaccat agttaattaa tcacattgat aatccaccac ctgaagtcct gccaacattc
     5101 agtccagcct tacccatgct aggagtctta accggggtgt cgttctgtgt ctccaccact
     5161 ggtgccgcat caggctctac aggaagctcc acagcaggcg cagggaccgg aggtgatacg
     5221 gatgtaccac caccagcgga gaagccctta gcgatggacc ggaggccttt actaatgctc
     5281 ttacccattg ctgttacgct tcggtcttgg tgaacttcag gcgtttgact acaggcagga
     5341 ctgcggttgc agcgccagtg tccagcttaa ctgtcgtagc agcttcagcg acaacgaaga
     5401 ccttaccgag gatttccaag tcgtgggact gagagtcgcc atcacgtgaa aggacgaccg
     5461 taatattctc accgtcgtga cggaagcagc cgaagctcag ggccgtgaag cgtttagcca
     5521 gcatgaacac acctttgtcc tcaccgttgt aatacgcgat gtcggtaccc agcatatcca
     5581 cgatagcgtc ttcaccagag aagccctgtt tagcagccag acgggacagc gctttcagtt
     5641 gggactcatg ggcgatgatg ccgtaatcag acacttcaga gccgaaagag gactcggtgt
     5701 tctcaaacag agcgtccatg atagcgtctg ccttagtggt caggcttcca gtcaggactg
     5761 gcagctctac ggtcttcagc agcttggagg actccagaac gtcaaccatg cgagcgttga
     5821 tggtacggac cacattctca cagatgttca tagcgtcctg ctggagtgtg ccatgagtga
     5881 aaccagtgga ggacttggcg tcccacagtt tagtttgctc cgagatgagg attgcctgtt
     5941 cgataggttc gacgacaatc tcctgaccag acggaacggt cacgacttca ggtcgctcac
     6001 caccgcgtac agggacgacc acagagcggt cagacagggt gtgggcgatg attggggtag
     6061 gcaggctatt gcgggtaggc aggacttcta cagggaacgg aatgttatcc tgagcgtctt
     6121 taagtacacc gttcagcata gccttaaccg tcttagctaa ctggagggca tagacctgtg
     6181 ggtcagcgtt agttggcaga ccttcgttaa cctgaatgtt gtcggtgtcg gtgatacgta
     6241 ctggctcaat gccgaactga tagctatctt taatcatggt cataataatc aatctcctaa
     6301 attagtttta gatactgtgg gtcccgacat caggtcagga ccacttgtgt gtctgtctta
     6361 gaggctgtcc atccagttac cgcctgactc aatgtcagcg acaatagcgg cacgggagcg
     6421 accagcggaa tgggtagcac tgcggaggtt ctcaccatag gtccggcgct cagctttgac
     6481 ctgttcgcgg cgctgtagct ctttctgcca ctctgcatcg gtcatgttct cttcacgcat
     6541 acgctcagcc tgaatgagct gctctttagc gatacgctca gcctcggctc gcttgttctg
     6601 ctggcgacgt tggtaggcgg tcatcaggtc attgctgaag aagtttccaa tggtcacgcc
     6661 agcttcagcc agagcttcca gtaattccat ggactcgcgg ctggtggcag gcagtcctga
     6721 caggtagccc ggaatcaggg tcttaatagc gaaggacagt gggtaggact gaccgtccag
     6781 actcacagtg ttttcgtaga gattgatgtc ggtgacatgg agtttccagt cattgttgtt
     6841 gaataacgca ctagctttgc tagacatagt aattactcct cttgagagtt gagactatcg
     6901 gtaccgcaga cacagttatg cctacgggtc tccccgcatc ggtcatctca aaaggtttaa
     6961 attaggggtt agtgagtggc gtccagctta gcgaacgctc ggcgtaactg attgttcagg
     7021 tcacggtcga ccagcaggtc tatcagttcg tctcgactat agaaaggacg ctcagggtcc
     7081 tctacgccca tccccagcat tgacaggagg tgacgggtag gcagaccacg gtaacggaga
     7141 gtagcctttc cgatgggtgt ccgtgggtcg atatgttgtt tagttgtcat tgttagaagt
     7201 cctcgttaga ccaatcatcc acgtcaaatt cactggtgat aggagcttgg aagttaggta
     7261 gaccctctgg tatctctaga ggcagtcgct cgtcgactct cagtgccatc tcttcgtgta
     7321 tatgccgaag cagagaagtc agtaagatag ggtccgcagg atgctcaggg ccgtgtctct
     7381 caatgaaaca tatggttgtc gctattgact ccataatcag ttcatcagtg agtcgcttag
     7441 aacccatagt cagacccctt gcccgctgct gctttctggg actttgtagc cttaccattc
     7501 aggtgagaat cca
//
LOCUS       DADWTH010000153         7457 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.156, whole genome shotgun sequence.
ACCESSION   DADWTH010000153 DADWTH010000000
VERSION     DADWTH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.156"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            215..291
                     /locus_tag="JNY02_003726"
     tRNA            215..291
                     /locus_tag="JNY02_003726"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /gene="envY"
                     /locus_tag="JNY02_003727"
     CDS             complement(337..1098)
                     /gene="envY"
                     /locus_tag="JNY02_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBB9435087.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="JNY02_003728"
     CDS             complement(1281..2171)
                     /locus_tag="JNY02_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBB9435088.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNY02_003729"
     CDS             complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNY02_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBB9435089.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKDPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5131..7368)
                     /gene="nrfB"
                     /locus_tag="JNY02_003730"
     CDS             complement(5131..7368)
                     /gene="nrfB"
                     /locus_tag="JNY02_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBB9435090.1"
                     /translation="MDWLLDVFATWLYSLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1737 a         1936 c         1951 g         1833 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatcgttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggatct
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag cgcggcgcgc
     3241 aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc ccgcgcaacg
     3301 taagcgttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag tgcgagttct
     3361 ggctgagctg ggatataacg ttgcgcatgc agccaccagt attgagcatt atttcccagc
     3421 tcacgttgtt ctgcctgttg aagccagcga tcgcgagccg cgccatttcc cgctgcctga
     3481 gcggtgttgg cggcggcgag cagatcctca ttgctcatgt cgtgaagact gattttctgc
     3541 caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc taccgcacga
     3601 tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg
     3661 ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc gtaggaaggc
     3721 gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc cggcaactga
     3781 ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc caccttcgcc
     3841 ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat taaagtctgg
     3901 ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc agcttcgcgt
     3961 gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag atttgtcggt
     4021 tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg ggtcgccacg
     4081 ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag cagtttttgc
     4141 gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac aacatactgg
     4201 cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt ggcgctgtat
     4261 ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag cggcttattt
     4321 tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa
     4381 gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg
     4441 tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc gcttaatgtg
     4501 ttctgctggc gtgcttcatt gtatagcgta tctgcctggg accattgttt caggtagatt
     4561 gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca
     4621 tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt ctgcccgact
     4681 tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc aagcagttct
     4741 tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg ctcaagtcgg
     4801 gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga
     4861 ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc cggcacctgt
     4921 tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt ctgtgccttc
     4981 agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag ccccagctct
     5041 tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt
     5101 aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg actccagttg
     5161 cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg aaacttgtaa
     5221 ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac
     5281 caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca cgttaattgc
     5341 tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact gatgcggcac
     5401 atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct gaaccgcatt
     5461 gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt
     5521 gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt tacgcgtcag
     5581 ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact catcattttc
     5641 cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt cggcaatcag
     5701 cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt gctctgcctg
     5761 cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac cgcccaggcg
     5821 tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga tgacctgatt
     5881 ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga cgctggggaa
     5941 gtcatgcgtt gttttatccc acgccacgcg acgcggatcg ccgtgctgaa gaacctgttt
     6001 cagcacgcgc cagttagcca taaagttaat caggttgccc cagaaaagac gcaggacgga
     6061 aagcaggccc tgcgtgaggc cgtagtagcc agtgacgaaa atcactcgct gcacgatgcg
     6121 gttaaccatc aagccaaagt ttagccacag cagggtcatt aaccatgcgc tgccgctaaa
     6181 aatagaaagg aaatgccagg catcgggcca caaactttca tacgccagca acagcaaaag
     6241 ctggatcatc accagcatcg cgaggaagct gacaaagtta ctgattgccc ctttgcggtc
     6301 gcgccagaga aagtagttca gcgtcaggct ggaggtccat ttatgggttt taaagccctg
     6361 gaaaacaatg ccgatgatcc agcgagattt ttgtcgaacc gcagtcgaaa aggtatcggg
     6421 gaaatattcg cgcacgcaga tcatgtttga cgtccgtgcg tgctgtaaaa atttacgctg
     6481 ctcgcgttct ttggcttcgt ccaccaccgg aaaacggaca aaaatttccg tcataccttt
     6541 ttctttcagg cggaagccaa tgtcgtaatc ttcagtaaga ctctgcacgt cgaaagcaat
     6601 accgttaccg tcagctaaca gtgcggtcac ggcgcggcgg ctgaaacagg tgccgacgcc
     6661 tgcgctgggc acttgtccgg cgagggcttc acgcaccgga acatctttgc catgcagttc
     6721 tgaaaactca tcaatgtaag tcatgctggt gaagtgcgtc cattcgcgtt caaacggata
     6781 cactgggatc tgaatcagat ctttacgctc gaccagatag ttgaacagac gcaattccat
     6841 cggtgaaatc acatcttcgg cgtcatgcag aataaaacca gcaaaagcga aattggcgct
     6901 acgttcaaac tgggtgatgg cgtccagcac gttgttcaga cagtcggctt tgctggtggg
     6961 accaggacgc gcgcagacta ccttatgcac gttcggaaag cgagcgcaca cttcgtcaac
     7021 atcacgttga gtatcggggt cgttggggta ggtgccaaca aagatatgat agttttcgta
     7081 gtcgagcgtg gtcgccgcca gctcggccat attgccgatg acgcccgttt cattccacgc
     7141 cggaaccata atcgctaacg gtttttcatc tggtttatac agttcgcggt aactcattcg
     7201 cgggtagcgg cgataaacac tcaacttgcg tttaatgcgg cgtacccagt agacgacatc
     7261 aataaaaaaa tcgtccagcc cgctgatgaa catgatgacc gctaacgtta tcgcgattac
     7321 ttttaagctg tagagccagg tagcaaaaac atcaagaagc cagtccacac aaaaacctta
     7381 cattaacgct ggttatgttt agggtggcgt atattaaggt tttttatgaa ttgtgacagc
     7441 tttttaccat taatagg
//
LOCUS       DADWTH010000154         7401 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.157, whole genome shotgun sequence.
ACCESSION   DADWTH010000154 DADWTH010000000
VERSION     DADWTH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.157"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..233)
                     /locus_tag="JNY02_003731"
     CDS             complement(<1..233)
                     /locus_tag="JNY02_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBB9435091.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="JNY02_003732"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="JNY02_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBB9435092.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="JNY02_003733"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="JNY02_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBB9435093.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="JNY02_003734"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="JNY02_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBB9435094.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="JNY02_003735"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="JNY02_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="JNY02_003736"
     CDS             complement(3305..3424)
                     /locus_tag="JNY02_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBB9435095.1"
                     /translation="MPLSYAGYHHFIMMSLVSAEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="JNY02_003737"
     CDS             complement(3385..3570)
                     /locus_tag="JNY02_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435096.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="JNY02_003738"
     CDS             3671..3844
                     /locus_tag="JNY02_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435097.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3846..4190
                     /locus_tag="JNY02_003739"
     CDS             3846..4190
                     /locus_tag="JNY02_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBB9435098.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="JNY02_003740"
     CDS             4194..4529
                     /locus_tag="JNY02_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBB9435099.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="JNY02_003741"
     CDS             complement(4586..6907)
                     /locus_tag="JNY02_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBB9435100.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTETTNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="JNY02_003742"
     CDS             complement(7024..7233)
                     /locus_tag="JNY02_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435101.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2031 a         1742 c         1648 g         1980 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtgtggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcagc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg ggggtaggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgttt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac c
//
LOCUS       DADWTH010000155         7297 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.158, whole genome shotgun sequence.
ACCESSION   DADWTH010000155 DADWTH010000000
VERSION     DADWTH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.158"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="JNY02_003743"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="JNY02_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBB9435102.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAVVGKLNRVNQE"
     gene            1158..2123
                     /gene="rbsC"
                     /locus_tag="JNY02_003744"
     CDS             1158..2123
                     /gene="rbsC"
                     /locus_tag="JNY02_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBB9435103.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2148..3025
                     /gene="rbsB"
                     /locus_tag="JNY02_003745"
                     /pseudo
     CDS             2148..3025
                     /gene="rbsB"
                     /locus_tag="JNY02_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3151..4080
                     /gene="rbsK"
                     /locus_tag="JNY02_003746"
     CDS             3151..4080
                     /gene="rbsK"
                     /locus_tag="JNY02_003746"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBB9435104.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4084..5076
                     /gene="rbsR"
                     /locus_tag="JNY02_003747"
     CDS             4084..5076
                     /gene="rbsR"
                     /locus_tag="JNY02_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBB9435105.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5042..6469)
                     /gene="mdtD"
                     /locus_tag="JNY02_003748"
     CDS             complement(5042..6469)
                     /gene="mdtD"
                     /locus_tag="JNY02_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBB9435106.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6492..7184)
                     /locus_tag="JNY02_003749"
     CDS             complement(6492..7184)
                     /locus_tag="JNY02_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBB9435107.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1845 a         1764 c         1929 g         1759 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgtt gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
     1861 ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact ggctatgagc
     1921 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
     1981 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
     2041 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
     2101 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
     2161 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
     2221 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
     2281 aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc
     2341 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
     2401 ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc
     2461 aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
     2521 acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag
     2581 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg
     2641 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
     2701 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
     2761 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc
     2821 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
     2881 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
     2941 cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
     3001 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
     3061 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
     3121 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
     3181 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
     3241 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
     3301 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc
     3361 gagagcgttc gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc
     3421 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
     3481 ggtattcatg ccggcgctaa tgctgctctt tccccatcgc tggtggaagc gcaacgtgag
     3541 cgtattgcca acgcgtcagc gttattaatg cagctggaat caccactcga aagtgtgatg
     3601 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
     3661 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
     3721 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
     3781 gtactgcatg aaaagggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
     3841 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
     3901 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
     3961 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
     4021 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
     4081 cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg tttctacctc aacagtttct
     4141 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
     4201 attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca
     4261 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
     4321 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc
     4381 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggctta
     4441 ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca
     4501 gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
     4561 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
     4621 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
     4681 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
     4741 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
     4801 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
     4861 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
     4921 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
     4981 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
     5041 cttactccga ttctgatgga acgcggttcg gcatagattt acgctgtctt ttgatcaaat
     5101 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
     5161 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
     5221 aaatgcgaag gacagccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
     5281 ccgccagaac attgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
     5341 tattcatcgc ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
     5401 gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa
     5461 tcaccgtgat ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca
     5521 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
     5581 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca cagccggtcc
     5641 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
     5701 ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag aatatagaga agcagtaacc
     5761 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
     5821 gctctattcc gcttgaaaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
     5881 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
     5941 caatacctat ggggatatta attaaaaata tccagtgcca ggttgcccag gtgaccagca
     6001 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
     6061 ataccggaag aagttcatta cgaggataag cacgcagtaa ggccagccga gcaacaggca
     6121 tcatcattgc gtcgcctatc ccttgaataa cccggaagac aaccagctgt ggtagcgaat
     6181 tagaaagtgc gcaggccaga gaacccaacg tgaacagact cacggcaagg gtaaaaatgc
     6241 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
     6301 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
     6361 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg
     6421 ccgcgatcca cggcaaaccc gacatactac gcttcttttt atcgctcatt caatgtcctg
     6481 ttatcgggtt atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
     6541 tcgccatcgc tttggataat cgcatcgaca atcgcctggt gcagatccag ctttatcact
     6601 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
     6661 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc tatatcgact
     6721 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
     6781 ttgagatgcg ccttctgttc cgcggtgcca accgttgctg ccagtaggca ggcttgcggc
     6841 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
     6901 atccaccaag taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccga
     6961 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
     7021 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg
     7081 gtgccgggtt catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac
     7141 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactta tcttcctttt
     7201 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
     7261 attgattgtt tgatggcaca ttaacgggac ttttgct
//
LOCUS       DADWTH010000156         7274 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.159, whole genome shotgun sequence.
ACCESSION   DADWTH010000156 DADWTH010000000
VERSION     DADWTH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.159"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            91..1211
                     /gene="yneK"
                     /locus_tag="JNY02_003750"
                     /pseudo
     CDS             91..1211
                     /gene="yneK"
                     /locus_tag="JNY02_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1361..2551
                     /gene="ydeA"
                     /locus_tag="JNY02_003751"
     CDS             1361..2551
                     /gene="ydeA"
                     /locus_tag="JNY02_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBB9435108.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2576..3241)
                     /gene="marC"
                     /locus_tag="JNY02_003752"
     CDS             complement(2576..3241)
                     /gene="marC"
                     /locus_tag="JNY02_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBB9435109.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3453..3887
                     /gene="marR"
                     /locus_tag="JNY02_003753"
     CDS             3453..3887
                     /gene="marR"
                     /locus_tag="JNY02_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBB9435110.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3907..4290
                     /gene="marA"
                     /locus_tag="JNY02_003754"
     CDS             3907..4290
                     /gene="marA"
                     /locus_tag="JNY02_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBB9435111.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4322..4540
                     /gene="marB"
                     /locus_tag="JNY02_003755"
     CDS             4322..4540
                     /gene="marB"
                     /locus_tag="JNY02_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBB9435112.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4571..5470)
                     /gene="eamA"
                     /locus_tag="JNY02_003756"
     CDS             complement(4571..5470)
                     /gene="eamA"
                     /locus_tag="JNY02_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBB9435113.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5734..6851
                     /gene="ydeE"
                     /locus_tag="JNY02_003757"
                     /pseudo
     CDS             5734..6851
                     /gene="ydeE"
                     /locus_tag="JNY02_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            6978..7073
                     /gene="mgtS"
                     /locus_tag="JNY02_003758"
     CDS             6978..7073
                     /gene="mgtS"
                     /locus_tag="JNY02_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBB9435114.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1897 a         1773 c         1652 g         1952 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tatccctttt agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca
      961 atatgttatt gcactatcac caatttatga atcccaatga aagtgatctg aatgacgtgt
     1021 tgatgccagc atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aggaccctca
     1081 aactgtttgt aaaaaatgaa acgccgccaa aagttactca cgaagacctg gtgaaaaaca
     1141 ggcctcgctc ccctgtcaga ccacctatac ccgcaaccgc caaaacgcca gacctccctg
     1201 aacgtcatta aaccgtgatg ttaccgactc tctgacgcgg aacagaatca gcgtcagaga
     1261 aacggaaaac gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga
     1321 tcatttcatc actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc
     1381 ccgcaaagtg gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac
     1441 caccgaattt gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac
     1501 cgctcaggtc ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc
     1561 ttttatgtta atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt
     1621 gtttattgcc agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag
     1681 tcgcattggt gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat
     1741 ccgtatggct ccggccggga agcgggcaca ggccttgagt ttaattgcca ccggtacagc
     1801 actggcgatg gtcttaggtt taccgctcgg gcgcattgtg gggcagtatt tcggctggcg
     1861 aatgaccttc ttcgcgattg gtattggggc gcttgttacc cttttgtgcc tgattaagtt
     1921 acttccctta ctgcctagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg
     1981 ccgcccggca ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac
     2041 ggcatacagc tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc
     2101 cacggcatta ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa
     2161 actgggtaat caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg
     2221 tctggcactg ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt
     2281 ctgggggatc gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc
     2341 accagatgct accgacgtcg cgatggcgct attctccggc atatttaata ttggaatcgg
     2401 ggcgggtgcg ttggtaggta atcaggtgag tttgcactgg tcaatgtcga tgattggtta
     2461 tgtgggcgcg gtgcctgctt ttgccgcgtt aatatggtca atcattatat ttcgccgctg
     2521 gccagtgaca ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat
     2581 ggtacgtttt aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca
     2641 gcaggaaccc catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa
     2701 ttgcgccgga gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg
     2761 gcggcgcaac catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg
     2821 agctaataat catcgctatg gttcccggac cggcggtact tggcattgcc agtggtacaa
     2881 aggcaatatt ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg
     2941 aatcaatcgc tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc
     3001 cgcctgcaat tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccag
     3061 cgtaatacgc caccatcatg atggcaaata cgtacaccga ggccatcaac gactgacgat
     3121 tacgttcggc actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg
     3181 ggttagctaa cggcagcaac accaccagcc ccaggccaat tgctttaaac aaatctaaca
     3241 ttggtggttg ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg
     3301 ataaagtgtc actctttagc taaccttgca tcgcattgac caaaacttga accgatttag
     3361 caaaacgtgg catcggtcaa ttcattcatt tgacttatac ttgcctaggc aatattatcc
     3421 cctgcaacta attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg
     3481 aaattattcc attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta
     3541 acgagtatct gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc
     3601 gctgcgcggc gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag
     3661 cactgacccg tatgctggat cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc
     3721 cgaatgataa gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca atatgcgaac
     3781 aatgccatca attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg
     3841 acgaagttgc aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt
     3901 atgacgatgt ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc
     3961 gaggacaacc tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc
     4021 aaatggcacc tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc
     4081 cgcagccgta agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc
     4141 tatctggcag aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat
     4201 tactttgatg ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt
     4261 ttacatccat taaatcatta caacagctag ttgataacgt gacaacgtca ctgaggcaat
     4321 catgaaacca ctttcatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt
     4381 tgcggaacaa accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc
     4441 atcgcaggaa caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga
     4501 tgcgctcggc gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc
     4561 tcggggctta ttaacttccc acctttaccg ctttacgcca ccgcaagcca aatacattga
     4621 tatacagccc ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat
     4681 ccaacaatag tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa
     4741 cccgccaggt ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg
     4801 ccacaaacgc cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt
     4861 gaatcatgga tgcggaacca tcgagaatca gcgaggcaac aaagaaggga atgattggga
     4921 ttaaagcgct ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct
     4981 ttttattgaa gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc
     5041 cgagcatcgc cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa
     5101 tcgctaaggc gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc
     5161 caagcacgat agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca
     5221 taccaaagtt aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat
     5281 accccaagag caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag
     5341 cgaccagcat aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt
     5401 tgatgaccac aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt
     5461 ttcgcgacat tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt
     5521 tacttttctt taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata
     5581 agtcgctata cttcggattt ttgccatgcg tccgatggtt aataacaacg acgaggcaag
     5641 aagggcgctg gtagagcgtc gtagggataa gttcatttgt ctgtccggca gtgcgtttcg
     5701 ttatgtttta ttttagagat gtaaagaaat agcatggcta cgctgccatt tatgaccatt
     5761 tacttgagtc gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg
     5821 ctcactattg gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag
     5881 aaacgctata tgttactggc aattaccgcc ttcccagcgg ttttattgcc attcctttag
     5941 tgaataacgt gacgctggtt gtgctctttt ttgccctcat taactgcgcc tattctgttt
     6001 ttgctaccgt gctgaaagcc tggtttgccg acaatctttc gtccaccagc aaaacgaaaa
     6061 tcttctcaat caactacacc atgctaaaca ttggctggac catcggtccg ccgctcggca
     6121 cgctgttggt gatgcagagc atcaatctgc ccttttggct ggcagctatc tgttccgcgt
     6181 ttcccatgct tttcattcaa atatgggtaa agcgcagcga gaaaatcatc gccacggaaa
     6241 caggcagtgt ctggtcgccg aaagttttat tacaagataa agcactgttg tggtttacct
     6301 gctctggttt tctggcttct tttgtaagcg gcgcatttgc ttcatgcatt tcacaatatg
     6361 tgatggtgat tgctgatggg gattttgccg aaaaggtggt cgcggttgtt cttccggtga
     6421 atgctgccat ggtggttacg ttgcaatatt ccgtgggccg ccgacttaac ccagctaaca
     6481 tccgcgcact gatgacagca ggcaccctct gtttcgtcat cggactggtc ggttttattt
     6541 tttccggcaa caacctgcta ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa
     6601 tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca ggaatgaaag
     6661 ccagctattt ttccgcccag tctttaggct ggcttggtgc cgcgattaac ccattagtga
     6721 gtggcgtagt gctaaccagc ctgccgcctt cctcgctgtt tgtcatctta gcgttggtga
     6781 tcattgctgc gtgggtgctg atgttaaaag ggattcgtgc aagaccgtgg gggcagcccg
     6841 cgctttgttg atttaagtcg aacacaataa aaatttaatt cagccttcgt ttaggttatc
     6901 tctgctaata tctttctcat cgagatgaaa attaaggtaa gcgaggaaac acaccacacc
     6961 ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta ctgggaataa
     7021 ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac taatgaacgg
     7081 agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc ctgattttta
     7141 tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg
     7201 ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc
     7261 atcagttttc tcaa
//
LOCUS       DADWTH010000157         7207 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.160, whole genome shotgun sequence.
ACCESSION   DADWTH010000157 DADWTH010000000
VERSION     DADWTH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.160"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..329)
                     /locus_tag="JNY02_003759"
     CDS             complement(<1..329)
                     /locus_tag="JNY02_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435115.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
     gene            699..1649
                     /gene="corA"
                     /locus_tag="JNY02_003760"
     CDS             699..1649
                     /gene="corA"
                     /locus_tag="JNY02_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBB9435116.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1690..2070)
                     /locus_tag="JNY02_003761"
     CDS             complement(1690..2070)
                     /locus_tag="JNY02_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBB9435117.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2084..2500)
                     /locus_tag="JNY02_003762"
     CDS             complement(2084..2500)
                     /locus_tag="JNY02_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435118.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYLSTFSMLMLAPTDSSKQ"
     gene            complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JNY02_003763"
     CDS             complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JNY02_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBB9435119.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3501..3968)
                     /locus_tag="JNY02_003764"
     CDS             complement(3501..3968)
                     /locus_tag="JNY02_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBB9435120.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4133..5002
                     /gene="pldA"
                     /locus_tag="JNY02_003765"
     CDS             4133..5002
                     /gene="pldA"
                     /locus_tag="JNY02_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBB9435121.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5135..6964
                     /gene="recQ"
                     /locus_tag="JNY02_003766"
     CDS             5135..6964
                     /gene="recQ"
                     /locus_tag="JNY02_003766"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBB9435122.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7028..>7207
                     /locus_tag="JNY02_003767"
     CDS             7028..>7207
                     /locus_tag="JNY02_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBB9435123.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
BASE COUNT         1850 a         1765 c         1806 g         1786 t
ORIGIN      
        1 tgctgaccgt tttcgatgta caaaccgagc ggcgcatagc tttcatcata gatgccgccg
       61 ttcatcgcca tctgcacctg accctgacta ttaatatccg ccagcagagc atgtaacgtt
      121 ccccacgctt cgccattggc tttttgccag tacattttca cccgctctgt ttgaggatta
      181 acggtatacg cctgtacggt cagcgtcgga tctgagagtg cgcaatcatc agcggcaaca
      241 gcaaacaagg gaagtaaggt gagggtgaga aaaatccgtt tgagattcaa ggtgatcatt
      301 cctttaccaa tgagtagccg atacgccatt ataggtcctg gatgtgggat ttttttatcc
      361 tgttagcgac cttgacgagt accaaaaagc gcgaagttca actattgttc tgtggtgttc
      421 tgttgcgtgt tgacggcaaa attttgctgg cgtaacatgc gcgcacgatc actctaagag
      481 gacattcgcc ttggacacac ccagtagata ctggctcact atcctgtcat ccaggatcaa
      541 ctcctaaggc tatccctttt tgctgatagc cttagcggtt gtcagcgacc ttaatttttc
      601 ccgtcgcgct gagtcaggct gtttaatggt ctgaaaccca atttgtttct gtgtgcccac
      661 cgaactgtcc gatattttaa gcattgggag tcccggtcat gctgagcgca tttcaactgg
      721 aaaataaccg actgacccgg ctggaagtcg aagagtcaca accccttgta aatgcagtat
      781 ggattgatct tgtcgaaccg gacgacgacg agcgactgcg cgtacaatct gaacttggcc
      841 agagcctggc aacccgcccg gaactggaag acatcgaagc atcggcacgt ttctttgaag
      901 acgacgacgg cctgcatatt cactccttct tcttctttga agatgcggaa gatcacgccg
      961 gtaactccac tgtggcattt accatccgtg atggtcgtct gtttactctg cgtgagcgtg
     1021 aactgcccgc ttttcgtctg tatcgtatgc gtgcccgtag ccagtcgatg gtagacggta
     1081 acgcctacga gttgctgctg gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa
     1141 ttgaaaatat ctatagcgac ctggagcagt tgagccgggt gattatggaa gggcatcagg
     1201 gcgatgagta cgacgaggcg ctctccacgc tggcggaact ggaagatatc ggctggaaag
     1261 ttcgcctgtg tctgatggat acccagcgcg ctctcaactt cctggtgcgt aaagcgcgtt
     1321 taccgggtgg gcaactggag caggcgcgtg aaatcctgcg cgatatcgaa tccctgctgc
     1381 cgcataacga atccctgttc cagaaggtga acttcctgat gcaggcggca atgggtttta
     1441 tcaacatcga gcagaaccgc atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc
     1501 cgccaacgct ggttgcttcc agctacggga tgaactttga gtttatgcca gaactgaagt
     1561 ggagtttcgg ctaccctggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc
     1621 tgtactttaa gcggaagaac tggttgtaaa aagaaggcgg tggcttagcc tggctaagcc
     1681 acctgttatt caaaggctcc aggtatttaa cccttttacc tctttctcat aaaaccattt
     1741 gttcgtgtta acagcaacat aggctgctac ggcaattccc agaatgttaa cgccaattaa
     1801 tgcaccaaca aatagagata gaatgccaag taatagaact ataattgctt ttttccataa
     1861 gcccagaaca aataaataga tccaggaaca gaagaaagca atgaaattca tttgaatagt
     1921 taatcgttgt cttactttta atgctttaaa tgctgcttta tattctggtg ttgctcccca
     1981 gaatccagga aagccatgtt gatcataaaa attaaatcgg tatttccatt tttcacttaa
     2041 tgaatcatcg ttcatatatt ccttactcat aaatactcca taactattgt tttgatgaat
     2101 cagtaggtgc aagcattagc atactgaaag tggaaagata acaaatcaaa aaaatcatcg
     2161 aaccattgcc tgaacaggca aaatcttcgg ctatcattgt gatgatagag atgatatata
     2221 ctgctaatgt accaaaaaca taaattttta tatatatgaa accactatca cggagtcgtt
     2281 ggcaattcat gttgatgacg agataatgga gtgcgatggt agagactata acaagaaaac
     2341 ctgcttcccc atcgttaaaa aagataataa gaaaggcaaa aatgaaattt attaaaataa
     2401 atgaaaatat atatcggcgt ctggaaatct taccgttaga tgttgggata aacatacgta
     2461 acataaattt tacatccttg tgtgagtctc ctgtcagcat ggcaatatgc ccactctcat
     2521 acaagagtcg gcatattttt tcagaatata tttatttttt atttggacgt tctacgctgc
     2581 gtataaatcg cgtccatcac aaaaattgcc agcgccaccc aaataaaggt gaaagtcacc
     2641 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
     2701 gggccaatgt actgaaaaaa gcctaacgtt gagagacgca ggcgcgtggc agcggcagta
     2761 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
     2821 atcgggtttt gccccatatg gctggttgaa ctgtcggcaa tagcaaacag gtaaatcgcc
     2881 gccacgggca gcagccacat ggtttcaatt aacatgccgg tttgtgcttc taccgcaatc
     2941 ttcttgcgca ccaggccgta gaaggcaaaa ctaaatgcca gccccagcgc gataataggt
     3001 agcgaaccaa aagtccacag ctggaccaac acgccacata tcgccagaat caccgccagc
     3061 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
     3121 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
     3181 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
     3241 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
     3301 ataaagaaaa acgaccagat cacgcgatgc gtcaggatct catcggcggg cacgtagtaa
     3361 atcagcttga agtatgctgg cgcaataccc caaataaaat aagcggcaag agcgagtaat
     3421 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
     3481 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     3541 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     3601 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     3661 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     3721 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcgc cgacgcaatg
     3781 actccgccgt gcaaaatgct ttgcgcccag ttacccacca tcatcggctg atttttaaag
     3841 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     3901 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     3961 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     4021 tgttggtttc tactttagga agggataact aacggcaatg gagttaagta tgtaaaatag
     4081 cccgcagaaa aacattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     4141 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     4201 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     4261 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatctacac
     4321 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     4381 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     4441 cccaaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     4501 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     4561 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     4621 ctctaacggg cgttcagacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     4681 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     4741 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     4801 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     4861 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     4921 cggctatgga gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtaggggt
     4981 tatgctaaac gacttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     5041 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     5101 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     5161 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     5221 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggttg ttatgcccac
     5281 tggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     5341 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactac aggccaacgg
     5401 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     5461 aggctgccgc accgggcaaa ttcgcttact gtatatcgcc ccggaacgcc tgatgctgga
     5521 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgtcg atgaagcgca
     5581 ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     5641 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     5701 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     5761 ccgtccgaat attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg
     5821 ctacgtgcag gaacagcgcg gtaagtctgg cattatctac tgcaacagcc gggcgaaagt
     5881 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     5941 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg atctgcaaat
     6001 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     6061 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     6121 tgatggcctg cctgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg
     6181 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgagcgcc acaaactcaa
     6241 tgcgatgggc gcgtttgccg aagcgcaaac ttgccgtcgt ctggtattgc tgaactattt
     6301 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     6361 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     6421 gtttgggatg ggttatgtgg tggaagtgat tcgtggtgct aataaccagc gtatccgcga
     6481 ctatggtcat gacaaactga aagtctatgg tatgggccgt gataaaagcc atgaacattg
     6541 ggtgagcgtg atccgccagc tgattcatct cggcctggtg acgcaaaata ttgcccagca
     6601 ttctgcccta caactgacag aggccgcgcg cccggtgctg cgcggcgaat cctctttgca
     6661 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     6721 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga
     6781 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     6841 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     6901 acgctttggt aaaccgttta tggcgctgat tcgtgcgcat gtcgatggcg acgacgaaga
     6961 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     7021 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag
     7081 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     7141 gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgttgct
     7201 tggcctg
//
LOCUS       DADWTH010000158         7186 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.161, whole genome shotgun sequence.
ACCESSION   DADWTH010000158 DADWTH010000000
VERSION     DADWTH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.161"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="JNY02_003768"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="JNY02_003768"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBB9435124.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /gene="hprS"
                     /locus_tag="JNY02_003769"
     CDS             complement(986..2344)
                     /gene="hprS"
                     /locus_tag="JNY02_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBB9435125.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLRELKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JNY02_003770"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JNY02_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBB9435126.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="JNY02_003771"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="JNY02_003771"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBB9435127.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="JNY02_003772"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="JNY02_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5309
                     /gene="msrQ"
                     /locus_tag="JNY02_003773"
                     /pseudo
     CDS             4675..5309
                     /gene="msrQ"
                     /locus_tag="JNY02_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5567..6217
                     /gene="zinT"
                     /locus_tag="JNY02_003774"
     CDS             5567..6217
                     /gene="zinT"
                     /locus_tag="JNY02_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBB9435128.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2034 a         1626 c         1509 g         2017 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgacag
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga gattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgagctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctaacaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcat tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa tcaattttta aaagaatcag atgttacagc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaggtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgctgggctg
     5221 gctgtactgc tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca
     5281 agcaggccca caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc
     5341 cggccggatg cagcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc
     5401 gtttgtcatc aacctttcat caccagaata atttatttta tttcaataag atagttcaat
     5461 acacctgaga ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac
     5521 aatataacat tacacataat gtacattaac tctggaggaa attgttttgg cgattcgtct
     5581 tcataaactg gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg
     5641 tcatcattct catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt
     5701 tgatgatacc aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt
     5761 ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga
     5821 taaaaccaaa acatttgctg aaattaaaga ttattatcgc aaaggttatc cgacagatat
     5881 cgaaatgatt ggcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc
     5941 ctgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt
     6001 tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag
     6061 cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc
     6121 ccagcaatct ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag
     6181 tagcgaagaa gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta
     6241 tttaacagaa cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca
     6301 aactcagaca cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg
     6361 agaaaaaggc gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc
     6421 caggagcaag tcatctgtca tggtaaagct aaccagaaat ataggttaat tttggtgttt
     6481 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
     6541 actcttggaa gttatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     6601 ggctcaatta ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag
     6661 tttcatagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     6721 gtagattttc gctcgaaacc ttcctgtcag ctccatagcg aatcaagcgc tgaatgtcac
     6781 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     6841 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     6901 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     6961 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     7021 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     7081 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     7141 gcttattccg ttttgcctgt gtgttgaata attccactgc agttat
//
LOCUS       DADWTH010000159         6924 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.162, whole genome shotgun sequence.
ACCESSION   DADWTH010000159 DADWTH010000000
VERSION     DADWTH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.162"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(374..517)
                     /locus_tag="JNY02_003775"
                     /pseudo
     CDS             complement(374..517)
                     /locus_tag="JNY02_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(851..1117)
                     /locus_tag="JNY02_003776"
     CDS             complement(851..1117)
                     /locus_tag="JNY02_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435129.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            1258..1464
                     /locus_tag="JNY02_003777"
     CDS             1258..1464
                     /locus_tag="JNY02_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBB9435130.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            1559..2161
                     /locus_tag="JNY02_003778"
     CDS             1559..2161
                     /locus_tag="JNY02_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435131.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            3361..4278
                     /locus_tag="JNY02_003779"
     CDS             3361..4278
                     /locus_tag="JNY02_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435132.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            4363..5235
                     /locus_tag="JNY02_003780"
     CDS             4363..5235
                     /locus_tag="JNY02_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBB9435133.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            5453..>6924
                     /locus_tag="JNY02_003781"
     CDS             5453..>6924
                     /locus_tag="JNY02_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HBB9435134.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLT"
BASE COUNT         1756 a         1761 c         1679 g         1728 t
ORIGIN      
        1 cagtgtgtgt atactgtgta gctggtgaga taacggttca tgtatcccct gagctgacag
       61 tgtaccgtgt atattaagcg taaccgacag gttctccttc ataccatatt aaattttcca
      121 gggattgccg ctgccttatt tcgtcccata caggaaaact catctgggtt tctgacaaat
      181 acgcgaaact cacagcccct caatatcaac cacacgttgt aagccggtaa tacctgaaaa
      241 tcagcctatt accgcccacg cctccggcac cattcccttt tgatttcgaa ttctttgttg
      301 tcgctatacc gcttaatgtt cccggtcctc cggtgcagat aacaccggag tcatcgtagc
      361 gctgcttcaa tattcaggtg acgtatttcc agacggaagc cggcgcgcga tcgtaaagtt
      421 tgttcattgt cagttctgcc agccggttgt caacggcaga gttaaaggct tccagctccg
      481 caagggtaag tttataacgc gtgtgggaat tcacttttcc cgcgtctcct gacgtattac
      541 gggctgccac ttacctggtg tcccgcaata cagccttagt gttccgttat cggggatccg
      601 gacgcaccgc aacaaccggg gaagtatttc tggtttagag cagtgtcaag agggatttct
      661 cagcccctca ctcaccggct cagtctcagg gcaggagcat taatacgctc agttcatgtc
      721 ttatacagga atgggcacag ggggagctgg cgtgtcactg ctgattttac gcatcagcgt
      781 tcatgtatct gcactctgat acatgaacga ttcacagcgc tctgttaccg gagagattta
      841 ctctcttagc ttactctggc aaatccttta cgttactctc tgatgacttc ttatatttat
      901 aataccaatc agacagcctt atcccatcgg tgatatcagt ctcctcctcg ccaggaacgg
      961 gctgactcac tgtaactacc tccgaaaaca actctgcaat tatcagaacg cctgcttctc
     1021 tccctgtcct cacgaaaact atcccctctt tatcgcgcgt gcgtgcggaa gcatcttttc
     1081 gcaacaacca cccggtatac cggtacggct cagccaccgc aacctccccg tttatctcct
     1141 ggcggtcaca ttcccgatta ttttttacgt ttctccccgg ttgttatgcc ggtgaaggtg
     1201 gcgcgctgtt ttcatgacca caccggctgg tttaacaaca tccggaggaa cattctcatg
     1261 gctaccccag tttcactgat ggatgaccag atggtcgaca tggcatttat cactcaactg
     1321 accggcctga cagataagtg gttttacagg ctcatcaggg atggggcctt tccggctccc
     1381 atcaagctgg gccgcagctc ccgctggctg aaaagtgaag tggaagcctg gctacaggcg
     1441 cgtattgcac agccccgccc gtaatttctg accgttatcc gttcacccgc agcagacgta
     1501 atccccggcc tgccggcggc attctgctgc ctgttatatc cccgtgagga atatgaaaat
     1561 gaaacaacag tatcagaccc gctacgaatg gctccacgaa agctaccaga aatggctgac
     1621 cggcttcacc cggcacgccg tatcctgggg cgtgtgtcat ccgaatatct actacttcca
     1681 taatctgacg cccggttggg tgtcattcaa tggtgaaaaa ccggagattg ccatcgtacc
     1741 ccagagcctg caccggctga tttatggccc tgacaaacgg gccacgccgc ccctggatga
     1801 tgatctgata gtgaatttat gcaccagtga acatctgctg gttcatcatc cgatgctgga
     1861 aggcattctc ctgtctgaat gtgaacgtct gaggcagcgt tctctggcaa ataaactgat
     1921 cagcctcttc cgtcagtttg gtggcacgga gttgcgcctc aaactggtct ggctttgctg
     1981 gcttgattta atgaccggaa acagccttga agactggaaa gagaacctga aacggaaatc
     2041 agaaaaagag ctggaggaat ggatcatcaa ccgtcagagg cagagcgcag cactgacgga
     2101 tctgatggat cagtacgtgc tcctggcata ccgcacaacg gttgacgata gccgcaactg
     2161 atgccggttg cttccggaag gtgtgacaaa atacggaaat gatgatgctc ccggccccca
     2221 tgctcacctg cagagcgtgt tcgtcgtcat catactgagc catgacgggc gtcttaaggg
     2281 catcctgtca ttgctgagca ggcaacacca ccagaggagc cacggacagg gatgttcttt
     2341 acagcccgat acacgccagg ttctcagcag actgaatgtg caggtgcggt tattcaccag
     2401 aaatggcatg ctgcggtaca tacccatcct ggtgacaata tgtacgtggc gacagcgatc
     2461 ccggcactgt ggaagagact cactcagcct gcaggatatt ctttccggca tccgggaatg
     2521 tccgcctgaa tgcaaacagg atgatatttg aatccgtaat actgttcttt catcataaat
     2581 aatttatgca gatacagtta accgttacag tagccggttt gttttaccgg gaaataatat
     2641 ccgtcagact gataccggaa catatctgaa acacgttatg atgttttgtt atcagaaatg
     2701 ttgtatattc ttatccgtgg cgacagttta atgccctctg gtatcacctg ataatgtata
     2761 tcctgcgatg attcgttata tttacgggac acgcctcttt tcattgtgct ccttttcaga
     2821 cttcattttc cgtaagtgtt ccccggcata aaggaggtac ctttctggcg tttataactc
     2881 tctttatcgg tgacgccggt aatacaggga ataccacacc gacaaacgag gtattaacag
     2941 acgatatttc agtgtccata atttttctgt ccggttaaat aacgtcagtc tgctgccctc
     3001 tgactgtaat catcaaagcc ggaatgcata ccgggttaca acgtaaaaaa agtgatgctg
     3061 gcccccggtg cagctggcgg gcgaaacatg tttacctttc aataccccct gtctgcagtc
     3121 tccactcttc catcctctta agctacgtcg gccacatgag tgcacttcca tgataacagg
     3181 tattcctgtc agcataacga agccggttac cttcccttcc tcctgcattt cctgtcagaa
     3241 tgactataca gcgcgcagat tgccttttta ttgaccatct taatataccg ttataaactt
     3301 ctttttatca tattattatc accttattat gtttaatttt taagcagtcg gagaaagagg
     3361 atggttcaga aaattttgtc ggataaggtt atgaatgaaa gaacaaatgc gtattactct
     3421 tattatcttg gagagcgtaa tatctccgta ctgcccctta atgtttatga tcccccggaa
     3481 cgttttatcg cttacataaa aaaaaacaga gaaaatctga acatcacact ttcagatttt
     3541 gaacttgaac aaataatttc tggtatgcgt ctgaaggccc tggccttcct ggtccccctg
     3601 gaaaaaatat cctggatcgc gggcagtgaa cgcgcatgct tattttcctg gtatctgttg
     3661 atgcagttta tccagaataa cagggcaaaa ataagcgcag acttgcttca aaagaataaa
     3721 ctgtatctta aagaagagta tctggaaggg aatgccttcc cgtcggactc atcgacacag
     3781 ttcagacaaa tactcagagt tctcgatatt ctttctgata agaatttgcg ggatgagtgg
     3841 ataatccaga ccaaagacag atggatgcgg gcatttaaat caaagagtcc tttcagttac
     3901 ctcctcccgg agaacgaaca tgaatgcata tggacatgga attatttgaa gggaaaaaat
     3961 attgcactgg aaaaactggc cagttttcct ggttctgctg atatttacca cgcaattcac
     4021 ctttcatttg acatatgggt tacctgtcca ctcacctcac ctgacgatat aaaaaatttc
     4081 agaaacagtt tcaataaggc caaggcgcag cgcaagtaca aaaaaatgca ggaagataaa
     4141 gtcaatgtac agttttttct tgacgcagaa accagggcgc agctcaaaga attatcgagg
     4201 gtcagaagac tcagtaccgg cgaaatgctg catgacctga ttgttgaaga gtataaaagg
     4261 taccgacatt ccagataatt tcagacatat attatctccg tgagtccccc gccacctttc
     4321 cggtgtgcgg gggttttgtc ttttttcacc gggaatacat gtatgaatcc gtctgatgcc
     4381 attgaggcaa ttgaaaaacc gctctcctcc ctgccttact cactttcccg tcacatcctg
     4441 gaacatctgc gcaaactcac cagtcacgaa cccgtgattg gcattatggg taaaagcggg
     4501 gccggtaaat cctcactctg taatgcactg tttcaggggg aggtcacccc ggtcagtgat
     4561 gttcacgccg gcacccggga agtgcagcgt ttccgtctga gtggccatgg tcacagcatg
     4621 gttatcactg acctgcccgg ggtgggcgag agccgggaca gagatgcaga gtatgaagcc
     4681 ctgtaccgtg acattctgcc tgaacttgac ctggtactgt ggctgattaa agccgatgac
     4741 cgtgccctgt ctgtggatga gtatttctgg cgacacatcc tgcaccgggg acatcagcag
     4801 gtgctgtttg tggtgacgca ggccgacaaa acggagccct gccatgaatg ggatatggcc
     4861 ggtattcagc cttctcccgc acaggcacag aatattcgcg aaaaaacgga ggcggtattc
     4921 cgtctgttcc ggcccgtaca tccggttgtt gccgtatcgg cccgcaccgg ctgggaactg
     4981 gatacgctgg tcagtgcgct aatgacagca cttcccgacc atgccgccag tcccctgatg
     5041 acccgcctgc aggacgggct gcgcacggaa tctgtccggg gtcaggcccg tgaacagttt
     5101 accggtgcgg tggaccggat atttgacacg gcggagagcg tctgtgttgc ctctgttgcc
     5161 cgtaaggttc tgcgcgctgt tcgtgacacc gtggtctctg ttgcccgcgc ggtatggaac
     5221 tggattttct tctgaacccg gtggtgatgc accagactct tgttacagat ttttaacccc
     5281 atgaaaacac aatgcaaaca cagccatacc cgggttgtcc ccttgcgttg tgtttctcaa
     5341 gttaatagaa tgcatcgatc gttactatca tatcgatcgc tataaacgat cgtttgtggt
     5401 aaatgacctc ctccgtggtt gtcatgaaaa tgcgtctcag gaaggttaaa taatgcacga
     5461 aaagaacatc gccctgcttt gtgatgaagc cgaccgactt ttgcaactga acattaatct
     5521 gctccggcaa atggttgatg aaccggatgt gttatctgac agtaagaacg aaaacagact
     5581 gctttttgat aaacagaaag cactgaaaag aattgaggag ctggagggcg aacaaatcaa
     5641 aaccgcccgc agggagatgg tgctggctgt tgtcggcacg atgaaagcag gtaaatcaac
     5701 taccatcaac gccattgtgg ggcaggaaat tctgcctaac cgtaaccgcc ccatgacctc
     5761 tgtaccgacg ctcatccgcc acgttcccgg aaaaactgag ccggtcctcc atctggaaca
     5821 tattcagccc gtccgcaatt tattaagcac actgcaacaa aaactcgaca ccccggcagg
     5881 acagcaagtc gcacagaccc tgcagcaaac cggggatacc tgcgaactgc tggatattct
     5941 ggcaaatgat ggctggctca aaaatgaata ccacggggaa gacgaaatct ttaccggact
     6001 ggcatcgtta aacgatctgg ttcgccttgc tgcggcaatg gggagtgagt ttccttttga
     6061 tgaatacgca gaagtgcaga aactgccggt gatcgacgtg gaattcagcc atctggtggg
     6121 gatggatgca tgccagggaa cactcacact gctggatacc cccggcccta atgaggccgg
     6181 acaaccgcag atggaagtga tgatgcggga tcaactgcag aaagcctctg cggtgctggc
     6241 ggtgatggat tacacccaga tgaactcaaa agcggatgaa gacgtccgta aagagcttaa
     6301 tgccattgct gacgtatcag ccggccgcct gtttgtgctg gtcaataaat ttgatgagaa
     6361 agaccgcaat ggcgatgggg cagatgccgt acgccagaaa gttccggcaa tgctgaacag
     6421 cgatgtgctg cccgcctccc gcgtttatcc aggctcatca cgtcaggcgt acctggctaa
     6481 ccgtgcgctt catgagttac ggaaaaacag aacccttcct gttgatgaag cctgggtcga
     6541 tgattttgtc agggaagcct tcggtcgcat gaaaaaagaa tacgtctgta aagacagtga
     6601 aatggcaacc gaaggggcga cagacctgtg ggaaggctca cttatcgatc aactgataac
     6661 ggaagtcata cagagctccc attccagagc ggcggcactg gcggttgatt ctgccgccgc
     6721 aaaactgatg cagaatgcgg aaaatgtcag cgaatatctg tcattacgcc atcaggggct
     6781 acagcaaagc attcagtcac tgcagtcgca tatcaccagc ctgcttgcgg atatccggga
     6841 aatcgcggac tgtcaggagc aggtgaccgc tgatgtcaga atggccatgg aggagatcga
     6901 tgccagaacc cgggaattac tgac
//
LOCUS       DADWTH010000160         6899 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.163, whole genome shotgun sequence.
ACCESSION   DADWTH010000160 DADWTH010000000
VERSION     DADWTH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.163"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..730
                     /locus_tag="JNY02_003782"
     CDS             <1..730
                     /locus_tag="JNY02_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB9435135.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
     gene            complement(1033..1803)
                     /locus_tag="JNY02_003783"
     CDS             complement(1033..1803)
                     /locus_tag="JNY02_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435136.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="JNY02_003784"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="JNY02_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="JNY02_003785"
     CDS             complement(2294..2515)
                     /locus_tag="JNY02_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435137.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="JNY02_003786"
     CDS             complement(2516..3199)
                     /locus_tag="JNY02_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBB9435138.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="JNY02_003787"
     CDS             complement(3263..3574)
                     /locus_tag="JNY02_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435139.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3571..4473)
                     /locus_tag="JNY02_003788"
     CDS             complement(3571..4473)
                     /locus_tag="JNY02_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBB9435140.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="JNY02_003789"
     CDS             4913..5872
                     /locus_tag="JNY02_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBB9435141.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="JNY02_003790"
     CDS             5872..6267
                     /locus_tag="JNY02_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBB9435142.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="JNY02_003791"
     CDS             6450..6830
                     /locus_tag="JNY02_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBB9435143.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1882 a         1780 c         1677 g         1560 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctgggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgctcaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcacagg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtag cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cgcaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgcg tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga cgccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat cgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggcgtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca cacagataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc cagcgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggttcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaagta gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgcttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgat
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg acccccgact gttaacgcta
     6061 atcagcggtt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaataaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgat
//
LOCUS       DADWTH010000161         6821 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.164, whole genome shotgun sequence.
ACCESSION   DADWTH010000161 DADWTH010000000
VERSION     DADWTH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.164"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            239..2671
                     /locus_tag="JNY02_003792"
     CDS             239..2671
                     /locus_tag="JNY02_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBB9435144.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGLFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2738..4207
                     /locus_tag="JNY02_003793"
     CDS             2738..4207
                     /locus_tag="JNY02_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBB9435145.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGNAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4207..5868
                     /locus_tag="JNY02_003794"
     CDS             4207..5868
                     /locus_tag="JNY02_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBB9435146.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="JNY02_003795"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="JNY02_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBB9435147.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..6797)
                     /locus_tag="JNY02_003796"
                     /pseudo
     CDS             complement(6477..6797)
                     /locus_tag="JNY02_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2013 a         1618 c         1559 g         1631 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgg
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcctatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca tgctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcaatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaaggaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga ataacactga ggtagcctga gtttaacgga c
//
LOCUS       DADWTH010000162         6781 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.165, whole genome shotgun sequence.
ACCESSION   DADWTH010000162 DADWTH010000000
VERSION     DADWTH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.165"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..3524
                     /locus_tag="JNY02_003797"
                     /pseudo
     CDS             1..3524
                     /locus_tag="JNY02_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6781
                     /locus_tag="JNY02_003798"
                     /pseudo
     CDS             3534..6781
                     /locus_tag="JNY02_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1797 a         1781 c         1634 g         1569 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 a
//
LOCUS       DADWTH010000163         6780 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.166, whole genome shotgun sequence.
ACCESSION   DADWTH010000163 DADWTH010000000
VERSION     DADWTH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.166"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            337..579
                     /locus_tag="JNY02_003799"
     CDS             337..579
                     /locus_tag="JNY02_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435148.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            693..1331
                     /locus_tag="JNY02_003800"
     CDS             693..1331
                     /locus_tag="JNY02_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBB9435149.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQSTLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1328..2065
                     /locus_tag="JNY02_003801"
     CDS             1328..2065
                     /locus_tag="JNY02_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBB9435150.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPFTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            2065..4161
                     /locus_tag="JNY02_003802"
     CDS             2065..4161
                     /locus_tag="JNY02_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBB9435151.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ERMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4208..4486)
                     /locus_tag="JNY02_003803"
     CDS             complement(4208..4486)
                     /locus_tag="JNY02_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435152.1"
                     /translation="MKRNLIKVVKMKPYFATLMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4487..4630
                     /locus_tag="JNY02_003804"
     CDS             4487..4630
                     /locus_tag="JNY02_003804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435153.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4756..5166
                     /gene="psiE"
                     /locus_tag="JNY02_003805"
     CDS             4756..5166
                     /gene="psiE"
                     /locus_tag="JNY02_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBB9435154.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="JNY02_003806"
                     /pseudo
     CDS             complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="JNY02_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
BASE COUNT         1698 a         1696 c         1785 g         1601 t
ORIGIN      
        1 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
       61 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
      121 tacgcagatt aattactttt gtttttattt taagtttatg atttttattg ttatttaaat
      181 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
      241 ctcgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
      301 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
      361 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
      421 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
      481 accgtaagct cggcggtggg tgtcgcgctc gcggtaaccg gtggcggtga tggttctaat
      541 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
      601 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
      661 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
      721 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
      781 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
      841 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
      901 aagacggaca acaaaaatgg gtcactcagg atcaatccac tcttgtcaca caacatggtc
      961 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     1021 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     1081 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     1141 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     1201 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     1261 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     1321 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     1381 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     1441 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     1501 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     1561 gctgacacgg ctggcagagc agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     1621 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     1681 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     1741 gtgggtcgga ccaccgccgt tcacggtcac gttgttcggg cttatcagcc atcctggcaa
     1801 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     1861 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     1921 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     1981 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     2041 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     2101 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     2161 gatttcggtg gcgtaggatt attacaaact cccaccgagc gtatggcacg ggaaggggag
     2221 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     2281 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     2341 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     2401 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     2461 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     2521 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     2581 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     2641 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     2701 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     2761 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     2821 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     2881 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     2941 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     3001 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     3061 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     3121 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     3181 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     3241 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     3301 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     3361 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     3421 gatttgtggc tgacggacca tctactgacc actggcagcc tgtttgcaaa tcttgccaac
     3481 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     3541 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     3601 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     3661 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     3721 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     3781 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     3841 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     3901 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     3961 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     4021 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     4081 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     4141 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     4201 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     4261 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     4321 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     4381 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagtag
     4441 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     4501 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     4561 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     4621 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     4681 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     4741 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     4801 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     4861 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     4921 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     4981 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     5041 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     5101 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     5161 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     5221 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     5281 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     5341 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     5401 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     5461 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     5521 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     5581 ccgagcctga gtgtaaaacg cggtactgag gctaaacata ccgattgcca ttccgagtgc
     5641 gccgataatt tgcagtggct tacgaccaaa tttatccaca gtcataattg ccagtacggt
     5701 aaaggtgagg ttgataactc cgacaataat ggtctgcaac agcgcgatat ccgtgctggc
     5761 ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc acattgatgc cgacaaattg
     5821 ctggaagatg gagagcataa cgccgattac aatcacgccc acgccaaaca tcagcagacg
     5881 accaccggtt ttgcggccat gatccaggga gtgtttaatt tcctgtactg cctgagttgc
     5941 aagcgtgttg cccataattt tgcgcaggat actttccgtc tgttcttgct tgccgcgtga
     6001 catcagccag cgaggacttt ctggcacggt atacagcagc attaagaaca gcagtgcagg
     6061 gatacattcc gaggcaaaca tataacgcct gccgtcagta ttcagccagc tggcatcacc
     6121 ggaacgggca ataaaatagt ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa
     6181 ctggttaaat gaaaccagtt ttccgcgaat atgagctgga gccagttccg caatatacat
     6241 tggtgaaagc attgaggcta aaccaacgcc aataccgcca ataatacgat aaataacaaa
     6301 ttccgggaca taacctgcca gataaatagg tactgtgttg tccgggttta tagaggtaaa
     6361 accaagttct ggccaggcag aacctacacc agaaataaaa aacaggacag cagaaatctt
     6421 aagtgaatca cgacggccga agcggttact gcaataacca ccgagggcac cgccgatgat
     6481 gcaaccaatc agagcgctgg ctacgcaaaa tcctaacagg gagttggcag cggattcact
     6541 taagttttgt ggagcaacaa agacggtatt gagtgactca acagtaccgg aaataacggc
     6601 ggtgtcgtag ccaaataata aaccacctaa tgtagcgact aaggtaatcg aaaatatata
     6661 actggaacta tactgggtat tcattcagac ctgccttaga ccattctgat cttttatcag
     6721 tgcattgatg ttggaattgt caggcagtta aggcaattat cattttttac aatgcgttta
//
LOCUS       DADWTH010000164         6777 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.167, whole genome shotgun sequence.
ACCESSION   DADWTH010000164 DADWTH010000000
VERSION     DADWTH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.167"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..90)
                     /locus_tag="JNY02_003807"
     CDS             complement(<1..90)
                     /locus_tag="JNY02_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HBB9435155.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            146..298
                     /locus_tag="JNY02_003808"
                     /pseudo
     CDS             146..298
                     /locus_tag="JNY02_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1679..2626
                     /gene="icsP"
                     /locus_tag="JNY02_003809"
     CDS             1679..2626
                     /gene="icsP"
                     /locus_tag="JNY02_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HBB9435156.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3188..3560)
                     /locus_tag="JNY02_003810"
                     /pseudo
     CDS             complement(3188..3560)
                     /locus_tag="JNY02_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3804..3923
                     /locus_tag="JNY02_003811"
                     /pseudo
     CDS             3804..3923
                     /locus_tag="JNY02_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4173..5039
                     /gene="ospB"
                     /locus_tag="JNY02_003812"
     CDS             4173..5039
                     /gene="ospB"
                     /locus_tag="JNY02_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HBB9435157.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5368..6108
                     /gene="phoN2"
                     /locus_tag="JNY02_003813"
     CDS             5368..6108
                     /gene="phoN2"
                     /locus_tag="JNY02_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HBB9435158.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6461..>6777)
                     /locus_tag="JNY02_003814"
     CDS             complement(6461..>6777)
                     /locus_tag="JNY02_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBB9435159.1"
                     /translation="KKADIALYLLSKFNFIKQDIAEMEKMNNNIYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRF
                     ER"
BASE COUNT         2131 a         1267 c         1315 g         2064 t
ORIGIN      
        1 aactctagga taaatatttc tcactatata ttcagtattt gcagtattta ttggaaatac
       61 gttcttaata ttcttgatgg gactaatcat tttaatctct atactcaaat ataaacatta
      121 ccatgaacaa tataattgtt cctaagtgga atgtctccac ggagaacact ttatctatcg
      181 ggaaatagtg cgggcaacag tgtttaatta tatcgagtgc gattacaatc gctggcgacg
      241 gcaccgttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc tcgcttaggc
      301 ctgtgtccat attacgtggg taggctcaca gagcctcact tacgactgcg tccatattat
      361 gtgggtagga tcaataaaga cgttttatat gtgtctgctt gcagccagaa aatttatacc
      421 ctgcaggtat cataaacatg cttgataact taattggggc tccctcttcc ttttatggtg
      481 taattgtttg attgaatact tccggggatt tcagtatgaa atgaagtata tttaatatac
      541 tttacaaaaa gtttgtatta gtggaataag gacaatagct tatccaaact gccaagcctc
      601 tttattataa gtaagatctg gcactggaat attctcaaaa ggactaacat catcaccacg
      661 accttcaacc aaccatttta ttgcatttaa atcccagaat agttcagtag tggtcaattt
      721 gggaaacgca caatattgta cggttacgtt atcgcaatag agtgaactcc cgccctatac
      781 ggcgttttca aggattagtc cttaatcgga caaccacatt aaagcataat aaataattaa
      841 gctttaataa tatactatag atacagttct attataaaaa atactatttt aggtgatgca
      901 ttaaattagt agaatattta ttcttttata aaagcaccaa tgaattgtcc aagtaaacat
      961 gaggtaaaat gtgtagagga taactgagtt tggtcacttt aactgtatta gtcgcagtct
     1021 gactctcgac tttaaaagga tgggaattac ctgtactgat agtgagaaag ctttccattg
     1081 aaataagtca aataaaagtt gatggcttca gtgctaaagt gaggtgcccg ctagccgtat
     1141 tcactcgaat cggaattcaa tgtattgctt ctgccatttc cataatattt cacgggaaaa
     1201 acaaaatctg aaaaactata cttaagtgtt gtattgagtc agttgagata caaatacata
     1261 tttatcagca cacgttacgt caaatacagg aggttgtcat gttggtactg aaaggcacgt
     1321 tggctattgc agccgtgatg acaatttatt gtcttatcgt tgttcttatg gaacgctttt
     1381 ctgattgatt tcatattggc gagagaacaa gaaggtgagt agaattgtcg cggaatcctg
     1441 aaactatcag cctcaggtat gaacaccaaa aggtaggtag agaaatctat ggcccccgtt
     1501 actttattcg cacaatgagg tattttttta tttttacaaa agttcaataa atattataat
     1561 aaaagatatg ttcttggtaa cataaacaaa tatttaatta aaataatgct tggcattgac
     1621 gtttatctta taaagtaaga agatcatgga catttcaacc aaaaaagtag agttctcgat
     1681 gaaatcaaaa ttccttgtac ttgcactttg tgtacctgcg atctttacta cacatgctac
     1741 cactaactat ccacttttca taccggacaa catcagtacg gatattagtc taggatctct
     1801 gagtggcaaa acaaaagaac gcgtttatca tcccaaggaa ggggggagaa aaattagtca
     1861 actggactgg aaatacagta atgcgactat tgttagaggt ggcatcgatt ggaagctaat
     1921 tccaaaagtg tctttcggag tttccggttg gactacttta ggtaaccaga aagcaagcat
     1981 ggttgataaa gactggaaca attccaatac ccctcaggta tggaccgacc agagctggca
     2041 tcccaatacg catctccgtg atgctaacga attcgagctg aatcttaaag gttggttatt
     2101 aaataatttg gattatcgac ttggactaat agcaggttac caagagagtc gttacagttt
     2161 taatgcaatg ggagggagtt atatttatag tgaaaacggg gggaacagga ataaaaaagg
     2221 ggcacatcct agtggtgaaa gaacaatagg ttacaaacag ctcttcaaaa taccttatat
     2281 tggattaact gctaattacc gccatgagaa ttttgagttt ggagcagaac tgaaatatag
     2341 tggttgggtt ctttcatctg atacagataa acactatcag actgagacaa tttttaaaga
     2401 tgaaataaaa aaccaaaatt actgctctgt tgctgcgaat attggatact atgtcacccc
     2461 cagtgcaaaa ttttatatag aaggctccag aaattacatt tctaataaaa aaggtgatac
     2521 atctctttat gagcaaagta ccaatatatc tggcaccatt aaaaatagtg caagtattga
     2581 atatattggt tttctcactt ccgcaggtat aaagtatatt ttttgaattg gttcaccgag
     2641 ttgattacgt tttcatggct aaaaattgcc gcccattgtg agtcatattc gccctgactt
     2701 tccagaacca tacgaactgc ccgttgacgg acttcggggg aaaaacgtgt atttttagtc
     2761 attctgttta cctctttctc agggcgttta gtctccagga tttccggggc ggttcaaaaa
     2821 actcttcgtt gattactgtg gacttactgt tggcattacc gaccctgaga ccggagaaat
     2881 aagaaccgct tagatcatcg tggctgttct cggggtatca agttacacat gggcagaggc
     2941 tacccaaggt ctactattat cgcctgaaag cgctgatgga acagtgctat cagggacatg
     3001 ctgatgaaaa tacctaaata atcagccagc actctatctt tccaaatcca cagcatagca
     3061 aagagagcaa aagagcctgt aaattcagaa attaaggaaa cgcctcttgg tgcacataat
     3121 ctatcttatg ttaaatgaaa tctaatttct tgattatatg ctggatcgtt cgtgggtggc
     3181 tttgccccta tatttccaga cacctgttat cattggcccg ctgccgcaga tattcccgtg
     3241 gcgagcgata atccagcgca ctatgcggat gccattcgtt ataatgctcg aatgcctctg
     3301 caaggttctt tgctgccgtt aacccgtcag gtttgggcat gatactgatg tagtcacact
     3361 ttattgtttt cacgaagctc tttgctattc tgttactctc cggactccgc accgccgtgt
     3421 tcttcggttc aacaaagtcc caacatccgg gtgaactggc gtgtttcatt agcccggtag
     3481 catgaaccat tatccgtcag tcactccact ggagacgacg gaagatcgtt gccgaagcgg
     3541 tgttccaccg ctcccagcat gtcgccaatg ggagagcatc tacggtctga ttgaactgtt
     3601 tattcaaatt gtccaccgtc tgtcagtacg ctctgaacgt cggctggtta aaacactgct
     3661 cgctgatttt cagagggttc acggtaaaac agcactttta ttccggattg cggaagcggc
     3721 tctgaataat ccggatgggc tggttaaaga ggttgtgtac catctgcata ttgttgaacc
     3781 agatcgcagc ggtaagagaa gttcatcata cttggcgcag ttacgcgatg tttctgctag
     3841 aggtgatgcc gtaaagaacg gcagaacatt accggaacaa gatagcggtc tacctgcact
     3901 ggtatcagac ccggggcttc cccggatgat ctccacggta ctttaggtac tgtgtctata
     3961 ttatgtaggt aggatcaatt aactttgtag ttgaaattct ttcattgaaa taatgaataa
     4021 aaaagacaaa ccgcattaaa caatgccaga atataattgc atttttgttc cgttttttta
     4081 aatcatagta tacaaagaag cttgcatact atatgaaaat ataacaccct ttactatgat
     4141 gtttataaaa caatatatgg agtcatgtag gtatgaattt agatggtgtt agaccatact
     4201 gtagaatagt caataaaaag aatgaaagca tatcagatat tgcatttgca catataataa
     4261 aaagggtaaa aaattcatca tgtactcacc caaaagcagc attggttttt ttaggagaga
     4321 aaggtttttg tgatagcaat gatgttctat ctattatggg acaacaaata ccaagagtat
     4381 ttaagaacaa gatgttatat gattatgttt ttaaaaatga aaaaagtaaa aatgattttc
     4441 taaaaatggc tgaatcatgg ctaccacaga gtgaaccaat agtaataaat aatgatgatg
     4501 acgcattgaa tgctgctgct tatttttctg taaaaaaagc gaaaataaaa acagtaaacg
     4561 atactgattt taaagagtat aataaggttt atattcttgg gcacggtagt cctggttctc
     4621 atcaattagg ccttggttcg gaacttattg atgtacaaac aatcatttca agaatgaaag
     4681 actgtggtat tctaaatgtg aaagatatcc gttttacttc atgcggctcc gctgataaag
     4741 tggctcctaa aaattttaac aatgcccctg ctgaaagtct ttcttgtatc cttaactctc
     4801 tgcctttttt taaggaaaaa gaatctttgc tagagcagat aaaaaaacac cttgaaaacg
     4861 atgagtcatt gagtgatggt ctaaaaatat ccggctatca tggatatgga gttcactatg
     4921 gtcaagagct ttttccctac tcacattatc gttcaacttc aattcctgct gatccggagc
     4981 atacagtaaa aagaagctct cagaaaaaga cttttattat taataaagaa ctggattagt
     5041 ataaaatttt taacctatag ttaattgttt gcatttaaga tgaagttatt ttatctttag
     5101 tagaaaagtg cttccctatg agggaaaatc acaaatcatc ataatacaga gacaaaacga
     5161 tacgaacaaa tagataaaaa acatcattct tttaccacat tatttttcgt gaatatgaaa
     5221 aataatgtta ttactttaat ataagactat tttttgtttt tccatcactc tgttcaaatt
     5281 tttccgcatg acttgtgttt tttgtaacac agctcgtttt ttacagctga ccaaaatcat
     5341 caattaatta tgctaaggaa ataaattatg aaaaccaaaa actttcttct tttttgtatt
     5401 gctacaaata tgatttttat cccctcagca aatgctctga aggcagaagg ttttctcact
     5461 caacaaactt caccagacag tttgtcaata cttccgccgc ctccggcaga ggattcagta
     5521 gtatttcagg ctgacaaagc tcattatgaa ttcggccgct cgctccggga tgctaatcgt
     5581 gtacgtctcg ctagcgaaga tgcatactac gagaattttg gtcttgcatt ttcagatgct
     5641 tatggcatgg atatttcaag ggaaaatacc ccaatcttat atcagttgtt aacacaagta
     5701 ctacaggata gccatgatta cgccgtgcgt aacgccaaag aatattataa aagagttcgt
     5761 ccattcgtta tttataaaga cgcaacctgt acacctgata aagatgagaa aatggctatc
     5821 actggctctt atccctctgg tcatgcatcc tttggttggg cagtagcact gatacttgcg
     5881 gagattaatc ctcaacgtaa agcggaaata cttcgacgtg gatatgagtt tggagaaagt
     5941 cgggtcatct gcggtgcgca ttggcaaagc gatgtagagg ctgggcgttt aatgggagca
     6001 tcggttgttg cagtacttca taatacacct gaatttacca aaagccttag cgaagccaaa
     6061 aaagagtttg aagaattaaa tactcctacc aatgaactga ccccataaag ctggacagcc
     6121 tgtatcaggc tatggagggc ccatagacaa atctacccta tatgagcata ggaggagtct
     6181 atgggcacac cacgttttac ccctgaattt aagggattac tggaaaggct gggacatatc
     6241 cctccggcag aagcagaaaa agcttattat gctgccatcg gaaacgatga tctggcaacc
     6301 tgagttcaca gataaaacat tctctaggaa actcggggcg gttccgttca ccacatgcaa
     6361 tgtggtgttg caggggaacg gtctgcacca tcccctatat cgatcaatat aacaaaaatg
     6421 acaacttcag attcagggca caacctaaat atattttagg tcacctctca aatcgtttgc
     6481 ctgataccgc tccatttttt aataaaaaat caatcatttt tgaatctttg cttttcattg
     6541 cattgtcaag catagtgtcc ccactatttg ctttttgaaa ttttttgttt acatcaacca
     6601 atccattatt gataaaatat tctagcactt tatagttggc accatcttta ctaagtaagt
     6661 attcaacatc atacagatta caatatatat tgttgttcat tttctccatc tctgcgatat
     6721 cttgttttat aaaattaaat ttagatagta aataaagtgc tatatcagct tttttat
//
LOCUS       DADWTH010000165         6768 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.168, whole genome shotgun sequence.
ACCESSION   DADWTH010000165 DADWTH010000000
VERSION     DADWTH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.168"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="JNY02_003815"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="JNY02_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBB9435160.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="JNY02_003816"
     CDS             2089..2238
                     /locus_tag="JNY02_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435161.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="JNY02_003817"
     CDS             complement(2983..3423)
                     /locus_tag="JNY02_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBB9435162.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="JNY02_003818"
     CDS             3641..4030
                     /locus_tag="JNY02_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435163.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="JNY02_003819"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="JNY02_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="JNY02_003820"
     CDS             complement(4585..5421)
                     /locus_tag="JNY02_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBB9435164.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..5696)
                     /locus_tag="JNY02_003821"
                     /pseudo
     CDS             complement(5421..5696)
                     /locus_tag="JNY02_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5771..6244)
                     /locus_tag="JNY02_003822"
     CDS             complement(5771..6244)
                     /locus_tag="JNY02_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBB9435165.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6265..6768)
                     /locus_tag="JNY02_003823"
                     /pseudo
     CDS             complement(6265..6768)
                     /locus_tag="JNY02_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1578 a         1753 c         1771 g         1666 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcgtcg
     5701 ctatcaatcg cggaaaaagg cgtagtgcgc gaagcgaacg aagctttttg ccgtcgattg
     5761 catagctttg ttaacccttt ttccaaattt gatagcaata gttaatgttt gaagtaaagt
     5821 gttgctcaaa aacaacttcg aaggtatttg gaatactcgg gaagaaaaca tccccctctg
     5881 gctcgatgtc gatcgtcgat aagtggagcg tagaggccat gggtaatgtt tctcggtaaa
     5941 tttctccgcc accagacact ataacgtgac cggtgaattc agctagcctg tccatggcct
     6001 cttcgattga ctgaaataca actacattgt catcatttga tgtccaacct gagcgggtaa
     6061 cgaccgcgta tttcctattg gggagtgcgc ccatagattc aaacgtcttg cgacccacca
     6121 gaagccactg attgtaggtc aatgctttaa aaagtagctg ctcccctttc gcggaccagg
     6181 gtatgtctgg accgcaacca atcacgccgt ttttcgcttt cgcagctatc aatgatactt
     6241 tcaaggttct catcctgggt taacggtgcg ctcttggtcg agccgcaccg gtagctctcg
     6301 ttcgacacga gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc
     6361 gggattgacg gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct
     6421 gcgctcaaac ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat
     6481 tgacggccac gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat
     6541 tgccgttatc accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca
     6601 agccacacct cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg
     6661 gcgggaagca ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc
     6721 accagattct ccgcctctga caggcaacca gtcagaatgc gattcacc
//
LOCUS       DADWTH010000166         6673 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.169, whole genome shotgun sequence.
ACCESSION   DADWTH010000166 DADWTH010000000
VERSION     DADWTH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.169"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..171)
                     /locus_tag="JNY02_003824"
     CDS             complement(<1..171)
                     /locus_tag="JNY02_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBB9435166.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTEVY"
     gene            complement(321..482)
                     /locus_tag="JNY02_003825"
     CDS             complement(321..482)
                     /locus_tag="JNY02_003825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435167.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(479..667)
                     /locus_tag="JNY02_003826"
     CDS             complement(479..667)
                     /locus_tag="JNY02_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBB9435168.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            927..1262
                     /gene="iraM"
                     /locus_tag="JNY02_003827"
     CDS             927..1262
                     /gene="iraM"
                     /locus_tag="JNY02_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBB9435169.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1543..1674)
                     /locus_tag="JNY02_003828"
     CDS             complement(1543..1674)
                     /locus_tag="JNY02_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HBB9435170.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2174..2250)
                     /locus_tag="JNY02_003829"
     tRNA            complement(2174..2250)
                     /locus_tag="JNY02_003829"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Arg,seq:tct)
     gene            complement(2352..2427)
                     /locus_tag="JNY02_003830"
     tRNA            complement(2352..2427)
                     /locus_tag="JNY02_003830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ile,seq:cat)
     gene            complement(2571..3392)
                     /locus_tag="JNY02_003831"
     CDS             complement(2571..3392)
                     /locus_tag="JNY02_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBB9435171.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3407..3763)
                     /locus_tag="JNY02_003832"
     CDS             complement(3407..3763)
                     /locus_tag="JNY02_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBB9435172.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3776..4825)
                     /locus_tag="JNY02_003833"
     CDS             complement(3776..4825)
                     /locus_tag="JNY02_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBB9435173.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNAQVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4827..5105)
                     /locus_tag="JNY02_003834"
     CDS             complement(4827..5105)
                     /locus_tag="JNY02_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435174.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(5172..5422)
                     /locus_tag="JNY02_003835"
                     /pseudo
     CDS             complement(5172..5422)
                     /locus_tag="JNY02_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="JNY02_003836"
     CDS             complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="JNY02_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBB9435175.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5867..6154)
                     /gene="relE"
                     /locus_tag="JNY02_003837"
     CDS             complement(5867..6154)
                     /gene="relE"
                     /locus_tag="JNY02_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBB9435176.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(6154..6393)
                     /gene="relB"
                     /locus_tag="JNY02_003838"
     CDS             complement(6154..6393)
                     /gene="relB"
                     /locus_tag="JNY02_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBB9435177.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            6418..>6673
                     /locus_tag="JNY02_003839"
     CDS             6418..>6673
                     /locus_tag="JNY02_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435178.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
BASE COUNT         1771 a         1784 c         1401 g         1717 t
ORIGIN      
        1 gtacacctca gtcagatatg tcagcagccc aaacagcaga ctccccagca cgcctattgc
       61 cgcccactga gacggggaaa acctgtccag caactgcagg aaccagtagc ccgttcccac
      121 cgctgacgtg gtgtatgaca cacctgttgt gattttttcc atctggtcca taccccgtct
      181 cccgttatcc ggaagctgac aacaataaaa aagccaccag ttaactactg atggctctga
      241 taactcatgc aagcgtctca gacgacccac tgacactacc ggtgagttta acgatacctt
      301 ccatttgact ggctcacttt ttatgatgat gccggtgcat ttatctccag caccagactt
      361 tctatctcaa cgccatacgt tgcatttttg gtaatatccg tcagcgtcag tgcatttagt
      421 cccactgcca gactgtcttt tatggcctgg aatgccgggc cagtacgatg acgtagtatc
      481 actccggctc agttgcacca ctgaccacca catcaccttc tgctgcaatc gcctgcatca
      541 gggtataagg ggttatggcc accggactac caaacggctg ccagccctct ttcagtttat
      601 gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca tagtgtttaa
      661 atgccatggt tctttccacc atctgaaaaa taattcttta aaatacctga catgtaatac
      721 agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga tatgcatgga
      781 taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa caacatcaga
      841 aaaataattc tgctctatgg tttaattcaa aaatatcatt tatacttttc agaacatcac
      901 cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca gttatccagc
      961 catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc
     1021 aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc
     1081 tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct
     1141 ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta ttgaatgaat
     1201 gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat
     1261 aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga
     1321 gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca
     1381 cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt
     1441 caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa
     1501 ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt
     1561 ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccgg
     1621 cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta
     1681 ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc
     1741 ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta
     1801 gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct
     1861 cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct
     1921 cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca
     1981 atattcactc tacccacacc atatgaatga taaatactta atgctgaaaa aaccattaga
     2041 ccgtataaca aatactcaat caatacttaa cagaactttt atttttgaca aatatataat
     2101 attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct
     2161 ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc
     2221 aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga
     2281 cccgcccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga taagctaatg
     2341 gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     2401 tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg taattaattt
     2461 cgcaataaaa aacccgctca gcggcgggtt gtagaaactc ttctaacgtc aggcataaaa
     2521 cgcccatcat tatgacgaat ttaccacaga ttccggaaaa atcaaccttg ttacctagtt
     2581 acctttttta actgccgctc agcccatgct tcttcaatat caaaccgggt caccagcgca
     2641 tcataaaatt tcttaactgt tttttcccat gacgcgcgtg ttatctggtt tgtcacctcg
     2701 catatagcat taaatgcctc cgttgatggt agtctttcat agccacgacc accacaacgc
     2761 tggcagtctc tgataacagg cataccacgt tttaccgact cttcacgatg aatggcgaca
     2821 ccacgcccac ggcaatcctt acaggcggtg gaaacctcac cctttccgcc acactccgga
     2881 caggcaactt ttaccacctc cctgactttt ttccattctt cccagtaaga cggatacaca
     2941 cctttcgtgc actttgccca taccggcggc ttaccatccg gatactggat cttgtttgta
     3001 aaaactatgc tttcaataaa tttttcccca tagcaacaag ggcactgctt tttactcgct
     3061 gcgctgcggg cataatcctc aaaagcgtac gaagccataa tgcgcatcac taccggtttt
     3121 atttctgccg gaagttttct caacgccgcc acacgatcgc accgactgag tgcataatct
     3181 gccagtaatt ctgttgcccg cgccctgtca ttcatactga tgcccatttt ccccaggaac
     3241 gcagaaaaac ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg
     3301 atactcagcg catcttttga cgttgaggcc gatgcatcgg tcaggccagg ggattttggg
     3361 gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgctcttaa tgcattatgg
     3421 ggtaaatctt cacccccaga cgtccaccag atactggctg accacgaacg atattgattt
     3481 catcaaactg ctcatcgtcc attaacactc ccgcatgcgt cagcgcatcc agcggtgctt
     3541 tcaggatatt gtccaggtcg cgacgacgct tatccggtgg ctctgcaatc acctttatcg
     3601 ccagccttcc ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc
     3661 ggcgataacg ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag
     3721 tgtttaccgt cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc
     3781 caagcacgcc agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac
     3841 catgcttttc ttcgaacgcc agaggatccg catgaagctc gttgtgatgc tcccgacaca
     3901 gcggtagcgt gaaaatatcg tgagattttg tccccattcc gccctgacca tgaccaatca
     3961 ggtgatgggg atcgtcggct ggcttaccac aacacgcaca cggctgtgtc ttcacccagc
     4021 gtttgtattt ctcgttaacc cagctgcgac gtttaggtcg tttcatgaaa gattccggag
     4081 actcaggatc aacggcaatg ctgaccaccg tcttttcctg ttgtgggttc tgttgctggt
     4141 gggcgtgagg cagcggcgca agattttttg tgcgctgttt cagtatgctg gtggcggtct
     4201 gctctcccgg tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc
     4261 ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc
     4321 accaggataa ttcagctaga gataattcac gctcctgcgt accgcttatt gcgtgaccga
     4381 tgacgtcaat catccatgct gacaggtttt gatgagcaag ttgctcgagt gattcggatg
     4441 tctggtcacg caactggttg tcgcagtgcc agcacaacac cattgcgccg gtaccataac
     4501 ggtgaatgac ggtttcgctg tgatgataat cgccgtgtgg ccactggcag gatttaatat
     4561 ggcgcaacag ccagtcagac aatgcaccag caccaccagc agcacgaatc acctgtgcgt
     4621 tactgaaaaa cggcagcaat gttttgtctt ccaccagcgg ctggcgaacg gcaggaacga
     4681 ccccggacgg cagattacgc atgcttttcg gttccggctc caccagtaac cgggtattgt
     4741 ggaataccgg catggattca cgaccgggct taaggaccac cagcccgagt tccggaacca
     4801 gaacaggtcg aagtaatacc cgcacgttac ctccagatcc gttgctggaa tgtgcgggac
     4861 ggacgcggtg gctgttcgga gtaagggagc ctgacggaga ttatccagtg acggtagtcg
     4921 aggctaaggg cttttttaac ctcgcatccg cgcctgcggt aacactgaat gagccattcg
     4981 gcctgttctt cagtgcatga gggatgctgg taccagtctg acttaaatgc gtgagaatac
     5041 cgctcgtgcg tgtgggcaag aacggtcgaa ttatcatgat tgtaatattt tgcgttgcgt
     5101 gccatcggtt ttctccggtg gcacggtgtt actcagcggg agttcagccc cgcgcaagat
     5161 tgtagatgag tttattcttc tgaaaaagca gaaaagccag cttttattcc ggtctctttc
     5221 aatgcctgta atgaagtgac aaactcaccg tcgcgcaaga taaatccgtc cgtcactcgg
     5281 gcatccacaa aattaattaa cgcagcccca ttttttgcaa acacacaatg cggtaatgac
     5341 taacaatatt tccattttca acgcacacag catagaggcc atcttcacaa aaaattttac
     5401 gcagttcttc gatgttcatc atcagaatcc ttccagataa gtagcactcc cctgttcggg
     5461 gtccatccct tttatccctg cgcgctactt aagtattttt gattctattc cggcaccatc
     5521 cagaacttca aatgcgttga aaataaaaac aaaaacccgc cgaagcgggt taagtgcggg
     5581 tgcgttgagg atgcctgcca catcagaggt ggcgagggat ttctcactcg ccgggtctct
     5641 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggattcgt
     5701 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
     5761 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctg tcaacgcaaa
     5821 gcagaagtgt caccttcggt gcgaaacaga gatgtcatgc tttggttcag agaatgcgtt
     5881 tgaccgcctc gctatatact tccgagcgtt ctcttttccc aacagaaatc acgaaaacga
     5941 caactttctc gtctataacc tggtatacaa ggcgatagcc tgaagaccgg agcttaatct
     6001 tgtaacaatc aggcatacca cggagcttgt ttgcttcaat ccggggtgac tcaagtactt
     6061 caaccagctt ctttttcaac tgttcacgta ccgtcgagcc cagctttcgc cattccttta
     6121 gtgcccgctc gtcaaaatcc agaaaatacg ccatcagagt tcatccagcg tcacacgtac
     6181 tggcttagga ttacgaagcc gttctttcac tatctccaca agttcagcat cttcatcact
     6241 caggagtgtc tgtttgaacg gcaagcgttc attgtcagcg atatactcga gcatgagacg
     6301 aagcgcttca gaaggagtta cacccatttt ttcaagcgcg gcgtaagaac gcgctttaag
     6361 ttcatcgtca atacgcaggt taatgctacc catgtcttac acctcttgta attacaaatg
     6421 tcattacaag tatcgcacta caacatgctt agggcaagtc acgaaggaag tcagaaagta
     6481 gtcgtaagaa cggtgatcac tgtccgcttt gtgccaggag cagccattgc taagtccatc
     6541 ctgtattgtg caggtcagct cttttttaaa gagtccggcc atcatcttac tggtacagac
     6601 accatatact ttgtgacggt caggctacat atgcacaact caacttattc atctattttt
     6661 tgctttagca tgt
//
LOCUS       DADWTH010000167         6485 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.170, whole genome shotgun sequence.
ACCESSION   DADWTH010000167 DADWTH010000000
VERSION     DADWTH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.170"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            370..939
                     /locus_tag="JNY02_003840"
     CDS             370..939
                     /locus_tag="JNY02_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBB9435179.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            1199..1600
                     /locus_tag="JNY02_003841"
     CDS             1199..1600
                     /locus_tag="JNY02_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435180.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            1588..2022
                     /locus_tag="JNY02_003842"
     CDS             1588..2022
                     /locus_tag="JNY02_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBB9435181.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            2377..2757
                     /locus_tag="JNY02_003843"
     CDS             2377..2757
                     /locus_tag="JNY02_003843"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBB9435182.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            2754..3101
                     /gene="tnpB"
                     /locus_tag="JNY02_003844"
     CDS             2754..3101
                     /gene="tnpB"
                     /locus_tag="JNY02_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBB9435183.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            3151..4536
                     /locus_tag="JNY02_003845"
     CDS             3151..4536
                     /locus_tag="JNY02_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBB9435184.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(4775..6133)
                     /locus_tag="JNY02_003846"
     CDS             complement(4775..6133)
                     /locus_tag="JNY02_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBB9435185.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT         1477 a         1747 c         1767 g         1494 t
ORIGIN      
        1 gctgacagta gtcagcagga ccacaataat cagcgccagc agtatggtaa cgatacggtc
       61 tgtattccgg tagtagccga aggggtcgac agaaccacaa tacgggcaac acactgtatt
      121 atgagtaatc gcgtttccac agtccctgca cttctttttg cgtatcacca tcactccctc
      181 ctcacagcct tacccgtaac cggtttttta cattaaaaaa cgacgctgaa aattcaaccg
      241 tcagtccgga gacgaccgtt cgggttatca cagagtgcct gagacagtgt cctgccggag
      301 gtcacttcag ggactctgcg tattttttac gacgtggcta ttccgtatga accatacgga
      361 gatttaacca tgacctacaa atacaacccc ttctggcagc aacgtattcg tgagacggtg
      421 cggcacgcac tgaatgttca tccccgcctg acggcattgc gggttgacct gcgtctcccg
      481 gatgtaccgg cagcaacgga cgcagctgtg atatcccgct tcatcaatgc cctgaaagcc
      541 cgaatcgacg cttaccagaa acgtaagcat cgggaaggta aacgcgtgca tcccacaacc
      601 ctgcattacg tctgggcccg ggagtttggg gagtgcaaag gtaaaaaaca ctatcacctg
      661 atgctgctgg tcaaccggga tacctggtgt cgtgccggtg attaccgcgc tccggagtct
      721 ctggccggga tgattaaaca ggcgtggtgc agtgccctgg gagtggatgt cgggtgccat
      781 gccacgctgg tgcattttcc ggcctggccg gcggtgtggc tggcgcgtaa tgatgacacc
      841 ggctttcagc aagtgctgga acgtgctgac tatctggcga aggagcatac caaagctcac
      901 tgcaccggtg agcgcaattt tggctgtagc cggagttgag cacgactggc gcgccaatat
      961 ccggcaaacc aaaagtatgg gttgttgtct ggcggcaacg atgtatccac tcgcccactg
     1021 actgtttttt acgtcagtgt tccattgtgt gacaaccaca gtgctgtaac agcaaccagc
     1081 ctgctttacc caaactccag tccagatttt aactgaaccg ccattcacac cctgaccacg
     1141 ctgcccgtac cgggacggct gttgcctgcg tgtcgtctgg cggctgaggt atatgactat
     1201 gtacgcaaaa tcctttatcg ctcttgatgg caacggacgt ctgacgggcg cccgtactgc
     1261 acaggccgca ccttatgcta actacacctg ccacttgtgt ggcagtgcac tcagatacca
     1321 tccgcaatac gaaactgaac ttccctggtt tgaacacact gacgacaggc tgacagagca
     1381 cggtcaacag tgcccttatg tcaggccgga gcgcagagaa atacagttga ttaaacgtct
     1441 gcagcaattc gtaccggatg ccttacccgt ggtgcgtaaa gccagctggc actgcagaca
     1501 atgtcaccac gattattatg gggagcagta ctgcacacac tgccagaccg gaggattcag
     1561 tattccccgg acaactcagg aggaaatatg cgaattctga actgttatat ggcgaatgac
     1621 agcaaaggcc attttgttac ggcgaaagaa gctgcgaagc acaaccgaca ggacgttttg
     1681 tgctgtgtgt cctgtggatg cccgttaaca cttcagcggg gcaatgacgg acaaccaccg
     1741 tggtttgaac atgaccagat gactgtcgct gaaaaaatcc tgctgcgatg tacctggctt
     1801 gacccggcag agaaagaggc ccgtcgtttg catctgcagg gcatgacggt tccggattat
     1861 acggtgaagg tgagaaagtg gttttgtgtg atgtgtgacg aagattatga gggggaaaag
     1921 tgctgcccac gctgcggtac cggggtatac agcagggcgt gggggcggca ggaggtgccg
     1981 tcggaagatg ccagggctga taatccgtta cagaggctgt aatggttgcc tccggagcag
     2041 tttgcggtga tgcctttccc gggaatggat tgccagtgcg gggactgtgg agcttaattc
     2101 cggtgttggt ggcctttccg ttgattttat gccaacagcc ccctgtgact gacaggctgc
     2161 ctcgtcattc cattcgtatc tccagtaaca gggggttgta ttgtatttta ttgtgccggt
     2221 tcaggtgtgc gtttcccggc gcgtctgcac cggcttaacc aaattcaaca gggataaaat
     2281 agtggtaagc gtacagcctg aaccgtctgg tcagaatctg acgaattaga caaagtggtg
     2341 tccaccaaat aagtagtggg aaccaaagtg tcagatatgc agaaaaatgt gactcccggc
     2401 aggcgaaaag gctgccctaa ttatcctccc gaatttaaac agctgctcgt tgctgcctcc
     2461 tgtgaacccg ggatatccat ctcaaaactt gctcttgaaa atggcattaa cgccaatctg
     2521 ttgttcaaat ggcgacaaca atggcgcgag ggaaagctgc tattaccttc ttcagagagc
     2581 ccccagctac ttcctgtgac tctcgatgca gctgccgaac agccagaatc gctcgcagag
     2641 gacccggaaa ccctcagtat cagctgtgag gtaacgttcc ggcacgggac gctccgcttc
     2701 aatggcaatg tcagcgaaaa gctcctgact ctgctgatac aggaactgaa gcgatgatcc
     2761 cgttaccttc cgggaccaaa atttggctgg ttgccggtat caccgatatg agaaatagct
     2821 tcaacggcct ggctgcgaaa gtacaaacgg cgctgaaaga cgatcccatg tccggccata
     2881 ttttcatttt ccggggccgc agcggcagtc aggttaaact gctgtggtcc accggcgacg
     2941 gactgtgcct cctgaccaaa cggctggagc gtgggcgctt cgcctggccg tcagcccgtg
     3001 atggcaaagt gttccttacg caggcgcagc tggcgatgct gctggaaggt atcgactggc
     3061 gacagcccaa gcggctgctg acctccctga ccatgctgta aatctcttta tcctggttgt
     3121 cacagaataa gcccggtaaa atacgggctt atgaacgaca tctcttctga cgacatcttc
     3181 ctgctgaaac agcgcctggc cgaacaggaa gcgctgatcc acgccctgca ggaaaagctg
     3241 agcaaccggg agcgcgaaat agaccatctg caggcgcagc tggataaact ccgccggatg
     3301 aacttcggca gtcgttccga aaaagtctcc cgccgtatcg cacaaatgga agccgatctg
     3361 aaccggcttc agaaagagag cgatacgctg actggtaggg tgtatgaccc ggcagtacag
     3421 cgtccgttgc gtcagacccg cacccgtaag ccgttccctg aatcactacc ccgtgacgaa
     3481 aagcgactgt tgcctgcggc gccgtgctgc ccgaactgcg gcggttcact gagctatctg
     3541 ggcgaggata ccgccgaaca gctggagttg atgcgtagcg ccttccgggt tatccggacg
     3601 gtacgggaaa aacatgcctg tactcagtgc gataccatcg tgcaggcacc tgcaccttcg
     3661 cggcccatcg agcggggtat cgccggaccg gggctgctgg cccgcgtgct gacctcgaag
     3721 tatgcagagc acaccccgct gtattgccag tcagaaatat acggccggca aggtgtggag
     3781 ctgagccgtt cactgctgtc gggctgggtg gatgcatgct gccggctgct gtctccgctg
     3841 gaagaggcgc ttcatggcta tgtcatgact gacggcaaac tccatgccga tgataccccg
     3901 gtccaggtac tgctgccggg taataagaag acgaagaccg ggcggttgtg ggcgtatgtt
     3961 cgtgatgacc gcaatgccgg gtcagcgttg gcacctgcag tgtggttcgc ttacagcccg
     4021 gacagaaaaa gcatccatcc gcagactcat cttgcttgct tcagcggtgt gctgcaagcg
     4081 gatgcgtacg ccgggttcaa cgagctgtat cgcaatggtg ggataacgga agctgcctgc
     4141 tgggctcatg cccgccgaaa gatccacgat gtgcacgtcc gcatcccgtc agcactgacg
     4201 gaagaagccc tggagcagat cggtcagttg tacgccatag aggcggatat aaggggaatg
     4261 ccggcagagc agcggcttgc tgaacgtcag cgaaaaacga aaccgctgtt gaaatccctg
     4321 gaaagctggt tgcgtgaaaa gatgaagacc ctgttcttcg gctctggcca tggtggtgag
     4381 cggggagcgc tactgtacag cctgatcggg acgtgcaaac tgaatgacgt ggatccagaa
     4441 agctaccttc gccatgtgct tggcgtcata gcagactggc cggtcaaccg ggtcagcgaa
     4501 ctgcttccgt ggcgcatagc actgccagct gaataacaca tccccgtcaa tacggccctc
     4561 gctgtacgct tacttttgtt cggactgctt atggcctttt tccactggtg gcgcaggaaa
     4621 gttaaccggc tgccaccatg ggatgacgtg taaaccgtgc aggcatgttt tcacgtggtg
     4681 acgctttcat tgggggtcta cgccggaacc ccaatattgt tcacatacat gaatttgaaa
     4741 aactaagcga tactgctgtc cgtaccttat aaacttattt caacgcttcc ggcagcgtca
     4801 caatccacag ttcactgtca gactccggtt tctccagtgg cttcactctg agggtcgttt
     4861 caacctgctt gtcgtcaagc gtcagctttc cttccgcgtt cacccgataa tccttgaatg
     4921 tcttgccctc gaccggatgt tgcatcgcca ccttcacgcc aaagtcgtta tcgttcgcca
     4981 ccgccagcgt tttgctgtcg atcagcgcca acccttcagc cttctcctgc tgccagccca
     5041 aggagcgcag atcaacaacc tgcgttttct gcgcaagggt aatgccgcgc tgtgacaggg
     5101 ttttctcatc atcaaactcc ggatactcac ccggcttgtc gaagcctgac aggtcgctcg
     5161 ccttattgag atcgacctcg taaatcaggt tacgcattct gttgttttta tctcttccct
     5221 gttcgatcag caggatgtgc tggttatcga gcgccacgat gtctccgatt ttagcgtcgc
     5281 tgtttttgct gtaggccgcg ctgtcgatag gataaccgta catcgcggtt tttccgcttg
     5341 ccggatcgaa gctcaccaga cgcgtaaaca gcgccttttt cttgctctta gcgtcgatat
     5401 ccagcgtact ttgcacggcg acaataatac gcccgtccgg catacgggtg aggccttcaa
     5461 agccgcggtt cgcctgacgc catttgagga tatttggcaa accgcccgca atcgcttttt
     5521 ccccttccgc cgcctgcgga ccgtggattg ccaggatttt ccctttactg tcgatgttaa
     5581 tcaggaacgg gccatactca tcgcacagcc agtaacctcc tttaccatcc ggagtgatcc
     5641 cttccgtatc cagcccgcga ttatccccct tcagtctatg taacgtgtcg ctgaaggcca
     5701 cttcattggt ggagccaatc acgtcgcttg ccagcggcag accgttgatc gcgcctttat
     5761 cgtcatgcag aggacgggga tcgatggcct ccgctttgcc gttttgtaca cgaatcgtca
     5821 tcagcagcgg cgcgaaatct ggcgtaacga aaattttagt ttcgtttttc ccctctttcg
     5881 gtgaatccgc gttcggaccg cgatcggtaa tggttgcgaa tgtcagggca tcgccctgtt
     5941 taccggtgaa cagcaggcca gaacctatcc caaccggaag gccgttcggg aatgcactgg
     6001 caaatgcgcc ggcgtagttc acgtgcgtgc cttctggaaa actgacgaca tagcgctcag
     6061 ccgtaggttg tgccgccagg gcagagaaag agagcgtaca accgattagc acgggaataa
     6121 tttttctttt catattgtta gcatctataa agtgaagatc acactacacc ataaagaaga
     6181 aacgagtcag gaaaatgaca tcgaatggca acatgcgcgg gcctgaggcc aaagaaatgt
     6241 aaaacactgt tgttatttgt aacaacggaa acatttacgg aaattatttg tctttcttcg
     6301 tgatcaaaaa tgctgtgtct tgttggattg gagtgatgag ggctgcgttt aggaaacgcc
     6361 ctatattgta cggttgcatt atcgcggtag gggtaatgca cgtgtcagta tgatgagctg
     6421 accacactca cgtgggtgga ctggtataac aatcgacgat tgctggaaag gcagggccat
     6481 atccc
//
LOCUS       DADWTH010000168         6307 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.172, whole genome shotgun sequence.
ACCESSION   DADWTH010000168 DADWTH010000000
VERSION     DADWTH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.172"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            73..378
                     /locus_tag="JNY02_003847"
     CDS             73..378
                     /locus_tag="JNY02_003847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435186.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="JNY02_003848"
     CDS             complement(454..918)
                     /locus_tag="JNY02_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBB9435187.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="JNY02_003849"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="JNY02_003849"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBB9435188.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEATASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="JNY02_003850"
     CDS             complement(1787..2476)
                     /locus_tag="JNY02_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBB9435189.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="JNY02_003851"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="JNY02_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBB9435190.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="JNY02_003852"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="JNY02_003852"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBB9435191.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="JNY02_003853"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="JNY02_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBB9435192.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1604 a         1705 c         1562 g         1436 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 gttgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgtgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttca gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaattacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaa
//
LOCUS       DADWTH010000169         6001 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.173, whole genome shotgun sequence.
ACCESSION   DADWTH010000169 DADWTH010000000
VERSION     DADWTH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.173"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            312..674
                     /locus_tag="JNY02_003854"
     CDS             312..674
                     /locus_tag="JNY02_003854"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HBB9435193.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            671..1588
                     /locus_tag="JNY02_003855"
     CDS             671..1588
                     /locus_tag="JNY02_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB9435194.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1601..2866
                     /locus_tag="JNY02_003856"
     CDS             1601..2866
                     /locus_tag="JNY02_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HBB9435195.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(2933..3556)
                     /locus_tag="JNY02_003857"
     CDS             complement(2933..3556)
                     /locus_tag="JNY02_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435196.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4307..4645)
                     /locus_tag="JNY02_003858"
                     /pseudo
     CDS             complement(4307..4645)
                     /locus_tag="JNY02_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            4767..5027
                     /locus_tag="JNY02_003859"
     CDS             4767..5027
                     /locus_tag="JNY02_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBB9435197.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5045..>6001)
                     /locus_tag="JNY02_003860"
     CDS             complement(5045..>6001)
                     /locus_tag="JNY02_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435198.1"
                     /translation="RRLVGDALHKNSGPYKDADRAALSRLYGSLADDQKAALSETNAL
                     RDFEVAQRLVQMRKSMEEQMINLTGRTLNGDVSRKATTALQAMSKGDAKGFRELMQNT
                     PSRKLRTELLGTGLRDMLSNGKRGADFNPAGFADWYQNMLANGQMRNLARHLPKETMS
                     GLNDVYKVAKAIKDAKSYEITTGRLNEFVKRFNRVTAANEFVANHAQRIGTAVGSTVS
                     GPFSAVGAVAGSEIGAKVASKIRAMGGAESIESAEKLISSPEFQKAARLAVKQAPESI
                     VDTTVRRSSAWRSFYNSLPESDKKTISRLGIMYWMNSDDNQK"
BASE COUNT         1694 a         1283 c         1189 g         1835 t
ORIGIN      
        1 ctacgacctt cgcattacga atctgtagca ccaatcataa ctatctgttt tagcaagcat
       61 taaccgcatt cactaagata tagttgatgg cacaaacaga aagttgatga atgatgttgc
      121 catatatatg tcacaaatac ggcacaacga tcttcaaaca tatagccaca cattcacaga
      181 agagcacaaa gccttgcaat ccagtgcaaa gatttgtgtg ccagagtttt tctcatcaac
      241 taccgcaagt atcgatcgat tgagacttgg atgatagact tcatgccttt gattattagc
      301 tgatagaaga aatgttaaag ctatttgcaa agtacacatc tataggcgtg ctgaacacac
      361 ttatacactg ggtggttttt ggtgtatgta tctatgccgc gcataccaat caggctatgg
      421 caaacttcgc aggtttcgtt gtggctgtga gttttagctt ctttgcgaat gcaaaattca
      481 cattcaaagc atcaactaca acgatgcgct acatgttata tgtcgggttc atgggaacac
      541 tgagtgctac tgttggatgg gttgctgata gatgttcact tcctccaatt gtcactcttg
      601 ttaccttctc cgccatcagc ttggtgtgcg gtttcgtcta ttcaaagttc attgtcttta
      661 gggatgcgaa atgaagatat ctcttgtcgt tcctgtcttc aatgaagaag aagcgatacc
      721 aattttttat aaaacggtac gtgaattcga agagttgaag ccatatgaag tagaaattgt
      781 tttcataaat gacggaagca aagacgctac ggagtcaatc attaacgctc tggctgtttc
      841 tgatccgcta gttgttccgc tgtcatttac acgcaacttt ggtaaagaac cagcattgtt
      901 tgcagggtta gaccatacaa ccggcgatgc cgtgattcca attgatgttg acctgcaaga
      961 cccgattgag gttattcctc atcttattga aaagtggcag gcaggtgctg acatggtgct
     1021 tgctaaacgt tcagaccgct caactgatgg acgactgaaa cgtaagacag ctgagtggtt
     1081 ctataaatta cacaacaaaa taagcacccc aaaaatagag gaaaatgtcg gagattttcg
     1141 actcatgtct cgtgaggttg tggagaacat taaactattg cctgagcgca atcttttcat
     1201 gaaaggcata ctgagctggg ttggtggtca gacagatgtc gttgaatatg tacgcacaga
     1261 gcgtgttgct ggcatctcaa aatttaatgg ctggaaatta tggaatctgg cactggaagg
     1321 tatcacaagt ttttcaacct ttcctcttcg cgtatggact tatataggct tgtttgttgc
     1381 aagcatttca tttttatatg gtgcatggat gattatagac accattgtct ttggtaaccc
     1441 agtacgcggg tatccctcca tgcttgtatc aatacttttc ctgggtggag tgcaactgat
     1501 cgggattggt gttctcggtg aatatatagg tagaatttat ttagaaacaa aatcaagacc
     1561 acggtactta attaaaagca gaaaataaca ggatgatctt atgaagatat tcataatgcg
     1621 caattggcac aagatatcaa tttttatact tgcatttact ttgatttggc ttaggcgcat
     1681 tgacattttg acaaatgccc agttttgggc tgaggatgca gttttttggt ataaagatgc
     1741 atacgaacaa ggttttttca gttcactcat aaccccaaga aatggttatt ttcaaacagt
     1801 atcaacactc attgttggtg cgacgacatt tattaatcca atttacgcac cattattatc
     1861 aaactttttt ggaataataa ttagagctat aattatatgg ttcttattta ccgatagatt
     1921 taaattccta agcaccacat caaaaatctt catctctgta tatttaattt gcatgcccgg
     1981 gcttgatgaa gtccaagcca atataacaaa tgctcactgg tatttatcat tgtatgttgc
     2041 aatgataata atatctgaag aatccaagtc aaaattatgg aaagcacatg atttattttt
     2101 tatagtatta tctggattga gcggtccatt tattatattc ataattgcat catctgtttt
     2161 taaatattta cacttatcaa aaggagtaat aagcataaga ggtttatact tattttatac
     2221 tcgactgcca tatttggcaa tgatcttgtg tgggctgata caggcaacat caataattct
     2281 tacatttaat ggaacaagat cacatgctcc attaggattc agttttgatg ttatgtcatc
     2341 tataatatca tctaatgtat ttcttttttc atttgtccct tggaatatcg ctaatgctgg
     2401 atgggataat caccttttat cgtacacact atcaacttta gtaattacat tatgtgtttt
     2461 catttatatt aaaggaaatt ggcaaatgaa agtgtttgcc acccttccaa tacttattgt
     2521 cgcattttct atggcaaaac ctcaactggc agattctgtt cctcaactgc caacacttgc
     2581 aaccggaaac gggtcaaggt attttgtaaa tattcatatt gcaatttttt cattaatatg
     2641 cgtttgtttg tttcaatgca taaagaataa aaccttaaaa atattcttta aaatctatgt
     2701 ttcagtcatc cttattgcaa tgataaaatt aaactttttt ataacccctc ttcctgacat
     2761 gaactggtct caaggagcag aattaataaa taaagcaaaa catggagaag ccgtctcatt
     2821 tacagtgctc cctccatggt taactcttga tttaataaaa aaataaaact tcacattaat
     2881 aaataacaat gaatccgcct ccctacgtat tcccgtaggg aggctgtcgt gtttatagta
     2941 gcctggtaat gctaaatgca aacgaactgg agattcctgc tccgctaagc gtattgcgca
     3001 gggtcaccct aaatgtatct acaccaatat aagtaacatc acaactaact gaatcaggcg
     3061 cagatatagg tgttatctga accacataac cattagtagc tgctgcgagc ggatgagaaa
     3121 acgttacatc atacacacca gtagcatgcg cgttaacgga tgaaacaccc ggcgcaatgt
     3181 ctacactacc attggcgtta attctccctg agcgcgtaac tgactctgca gaaaccgatg
     3241 tggccttact ggttatttcc ataaaggtgc aaccaatatc gatcaattcg caattgttcc
     3301 ctgttatcca gtatggtcga tctgcacttg gctgataatc ccctgcgctc tggaacgttg
     3361 tgccattgag taagactata acttttcccc cacctgagct tgttacttgt atattcgtgt
     3421 gcgtgtagcg ttcactgctc acgcgatgaa aatttccacc gtttataact accgttacag
     3481 gtcgcgtccc agtattatct atagcaaggt ctgccccccc ggcattagcc tcgaagtatg
     3541 ggttgtttaa caccaaagca cacgaaccgt taaggccatt tatggctaat tgcgcaccac
     3601 ctgtaccgga atccccctga gtaccatttc cttcacaagt caggctgttg atagtagttc
     3661 ctgcccccca tctatcacca agtatacccc atcgtgtatt ctggcttgca gttaatcggt
     3721 taactgtaat ggcattaagc tctgaccctt cactggagtt aagtgcgcgc cagccaatac
     3781 ggttaccctg taagtacaca ttatctaact ctgacgttaa caccgagtct gtacgcaacc
     3841 cttcgccggt agtgtgccca taagacacaa tgttctctaa tttagtgtat gctttattgg
     3901 ttacgagaag accgtaagac gatcccgaac ctttaagtgt aatgtccccc agataatcat
     3961 aggcgtgtac acgttgggtt gccccgactg tatcaccgag catctttaag ttgaaatccg
     4021 aggcatggca gttaaatatc gtttctgcca tattttctcc ggaataatga gcacggactc
     4081 ctgtcgaacc aataaattta gcgtcatagt tgccgtaatc aagaacgagt ggtgacgcga
     4141 tatcgtacac cccttgcttt atttttaatg ttccaccgaa aggccacgat gtaagcgggt
     4201 cttgatatat ttcatttttt attgcaaata gctcctcttg gagatttttg ccgctattag
     4261 tgccaatgtg agcagctcct tcatctgatt gaagtaactg ctttaactga tcaggatcgt
     4321 acttcagcac attctgaaaa tagaactgct gcgaaccata cgcatcatat acagccattg
     4381 aatggccttg ctcagttaca aatttggcaa tctgcccgtt gtacacagga tatccggcag
     4441 cattgataac aattggctga gaaacaggga cgtgagatcc gtcttcattc tctacataaa
     4501 cctgaatctg gttttcagga tttaccgggt cagtgtcaat ttttccgata taaattttgc
     4561 cattggatac ggctttaaaa gaacgcgcca tagtgaagag ttgcgaaggc atgctgacca
     4621 caacatttgc ggcgatatct gacatttcat tgctccagac gaatgatatg atgcaatcat
     4681 gatgtgattg cataccgaaa tggcactatt gaatatttat ccagtaggtt acgatgccat
     4741 tccacccaac tggtgaggca tcaaggatgt acagcaaata cgacgaggca cagtttcact
     4801 tgagacttcc gcatgaactc cacgcgaaaa ttaaacagtg tgcgaagatg aataacagtt
     4861 cgctgaactc agagataatt gcagcgattg aagaatcatt ggctaaacaa agctctgcat
     4921 cagtttacat tgacgatgca gagcgtatgg cagaacaaca atctgatatg gttaagaaaa
     4981 ttgtctttga tacgctcaag gagctatata aaaaagacag caactaacca tccgtggctt
     5041 tccgttactt ctggttatca tcactgttca tccagtacat gatgcctagc cttgatatgg
     5101 ttttcttatc tgattctgga agtgagttgt aaaacgagcg ccaagcagaa gagcgtctta
     5161 cagttgtatc aacgatgctt tctggtgctt gttttactgc cagccttgct gctttctgga
     5221 attctggtga gctaattagc ttttctgcag attcaattga ttcagcgccg cccatcgccc
     5281 tgattttgct ggcgactttt gccccaattt ctgacccagc aacagcacct actgcactga
     5341 acggtcctga cacagttgaa ccaaccgcag tgccaatgcg ttgggcatgg ttagcaacaa
     5401 attcatttgc cgcagtgacg cgattaaacc gtttgacgaa ctcgtttagt cttcctgtag
     5461 ttatctcgta agattttgca tctttgatag cctttgcgac cttatataca tcgttcagac
     5521 ctgacatagt ctcttttggt aaatgtctgg caagattgcg catctgcccg tttgctaaca
     5581 tgttttgata ccagtcagca aaccctgcag gattaaaatc agcgccacgt tttccgttcg
     5641 aaagcatatc ccgaagacct gttcccagta gctcggttct tagcttcctg gacggcgtgt
     5701 tttgcatcaa ttcacgaaat cctttggcat cgcctttcga cattgcctgt agtgctgtag
     5761 ttgctttgcg agaaacatca ccgttcagcg ttctgccagt tagattaatc atttgctctt
     5821 ccatgctttt tcgcatctga acaagcctct gagcaacttc aaaatcacgt aatgcatttg
     5881 tctctgacag cgccgccttt tgatcatcgg cgagcgaacc gtaaagcctc gataaagcag
     5941 cgcgatcagc atctttatat ggtccagaat tcttatgaag tgcatcacca acaagtcgtc
     6001 g
//
LOCUS       DADWTH010000170         5994 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.174, whole genome shotgun sequence.
ACCESSION   DADWTH010000170 DADWTH010000000
VERSION     DADWTH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.174"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..268)
                     /locus_tag="JNY02_003861"
     CDS             complement(<1..268)
                     /locus_tag="JNY02_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBB9435199.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /gene="mtlA"
                     /locus_tag="JNY02_003862"
     CDS             805..2706
                     /gene="mtlA"
                     /locus_tag="JNY02_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBB9435200.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="JNY02_003863"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="JNY02_003863"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBB9435201.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="JNY02_003864"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="JNY02_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBB9435202.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="JNY02_003865"
     CDS             complement(4548..4757)
                     /locus_tag="JNY02_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435203.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="JNY02_003866"
     CDS             5041..5403
                     /locus_tag="JNY02_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBB9435204.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1565 a         1454 c         1488 g         1487 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aagccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctc
//
LOCUS       DADWTH010000171         5940 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.175, whole genome shotgun sequence.
ACCESSION   DADWTH010000171 DADWTH010000000
VERSION     DADWTH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.175"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JNY02_003867"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JNY02_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBB9435205.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JNY02_003868"
     CDS             578..1027
                     /locus_tag="JNY02_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBB9435206.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1260)
                     /locus_tag="JNY02_003869"
                     /pseudo
     CDS             complement(1028..1260)
                     /locus_tag="JNY02_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1346..1519)
                     /gene="ortT"
                     /locus_tag="JNY02_003870"
     CDS             complement(1346..1519)
                     /gene="ortT"
                     /locus_tag="JNY02_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBB9435207.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1714..1809
                     /gene="yncL"
                     /locus_tag="JNY02_003871"
     CDS             1714..1809
                     /gene="yncL"
                     /locus_tag="JNY02_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBB9435208.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2211..3020)
                     /locus_tag="JNY02_003872"
     CDS             complement(2211..3020)
                     /locus_tag="JNY02_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBB9435209.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNY02_003873"
                     /pseudo
     CDS             complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNY02_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4677..5471)
                     /locus_tag="JNY02_003874"
     CDS             complement(4677..5471)
                     /locus_tag="JNY02_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB9435210.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="JNY02_003875"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="JNY02_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1555 a         1507 c         1320 g         1558 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagccg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcgttggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg tgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggccgctt ccatatcttc
     1081 tttatacttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg
     1141 atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc
     1201 aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat
     1261 actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata agtctgcgtg
     1321 gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac
     1381 tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt
     1441 gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca
     1501 tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc gcgcaaatat
     1561 gcgccatttt ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag
     1621 caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta
     1681 tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta
     1741 ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg
     1801 tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac
     1861 agtacgcaag atcttctcat actctccaag tttgcaacat tatccttaat atttacttct
     1921 ttttgctggc atctacattg aaatgagtag aaaacatccc cctactgtcg tctatacaaa
     1981 cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa tactttatac
     2041 gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc
     2101 gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc ccattgaaat
     2161 tgcaaacccc gccctggtta tcttgcccat gtatcatgca aaacagataa ttataggcct
     2221 ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt tccatttgat
     2281 atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt attatccgga
     2341 agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat aacatttgaa
     2401 tcaaacctaa atctgattat ttttgcagga actcctgcaa ctatggcata tgggggcaca
     2461 tctttggtaa caacggaatt ggcagcaata acagcaccat cacctattgc gatgccacct
     2521 ttcaatacaa catcatttcc tatccagaca tcatttccta taatcggatt ttcaggtttg
     2581 gtaatgaatg gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa cttatcaaat
     2641 tcaccgttat aagtcatcat gtgagtagta aaacgattca aaggatgatt tccacccatt
     2701 atttttacat ttgatgcaat actacaaaat cgacctatac gtatattccc tgaaaaatgg
     2761 caggtcggaa atgaaaaagc gccgacactg gagaaatttt tcacaggcat tgttgaatat
     2821 tcttcagcaa taacattatc ttcaattgtt atcttgtcac cttttgagaa aagaaataaa
     2881 ttcctttctt tatacgactt cttcccctta tgatttaaaa agattttatt atccatacaa
     2941 aactcaaagt gttttttcgt ccatctgaag gtaagggttt tatttttatc ttccgtgaat
     3001 ttgtacaatt gaactttcat tgttaatact tctacttata aattattagt ttaataacga
     3061 tactctaaag ccttatgcta atcaactgtg acaggatgaa aaatcgccat caaacattta
     3121 gcctaatttt tacgccagtt attaatgtaa tcattctaat acttaaccgc cattaaactc
     3181 agaacctaaa acgatagtgg ccgtcctata acagccacta acatctaact taatgtttaa
     3241 ccatgacgtg gcggacgacg gtgtaatcct ccagcccata aagtgacata tccttgccgt
     3301 aaccagaaag tttctgccca ccgtgcggca tttcacttac cagcatgaaa tgggtattga
     3361 cccaggtaca accatattgc agccgtgcgc tgacgcgatg cgccctgccc acatctttcg
     3421 tccataccga agatgcaagt ccgtactggc tgtcattcgc ccagttcacc acctgttctt
     3481 cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac ctctttttgc acgatggcat
     3541 cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta atagccatta cccttgcgct
     3601 tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc
     3661 tgacgcgttc gagatgcgcc agcgagctta aaggtccaag ctccgtagac tcgtcatccg
     3721 gcgcaccaga ttttaacgtt gccaccgcag gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaaaata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatgc ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 accagttctc cgttaatcag taacttatgt tgcatagatt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 tagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
//
LOCUS       DADWTH010000172         5857 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.176, whole genome shotgun sequence.
ACCESSION   DADWTH010000172 DADWTH010000000
VERSION     DADWTH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.176"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            83..442
                     /locus_tag="JNY02_003876"
     CDS             83..442
                     /locus_tag="JNY02_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBB9435211.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(562..741)
                     /locus_tag="JNY02_003877"
     CDS             complement(562..741)
                     /locus_tag="JNY02_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBB9435212.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            815..2176
                     /gene="pabB"
                     /locus_tag="JNY02_003878"
     CDS             815..2176
                     /gene="pabB"
                     /locus_tag="JNY02_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBB9435213.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2180..2758
                     /locus_tag="JNY02_003879"
     CDS             2180..2758
                     /locus_tag="JNY02_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBB9435214.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHTGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2942..4306
                     /gene="sdaA"
                     /locus_tag="JNY02_003880"
     CDS             2942..4306
                     /gene="sdaA"
                     /locus_tag="JNY02_003880"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBB9435215.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4437..>5857
                     /locus_tag="JNY02_003881"
     CDS             4437..>5857
                     /locus_tag="JNY02_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBB9435216.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPEATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVA"
BASE COUNT         1424 a         1464 c         1470 g         1499 t
ORIGIN      
        1 cattatctga acataaaaca ctatcagtaa gttggagtca ttaccaactc gtgccatatc
       61 gttataatcc ctttgatatt agatgcaaat taaggtcata tatagcctta tcgataacat
      121 ggttaatttt aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg
      181 tgccatggat gtatcagcgc caacaccaag ttcgatgaat gaaagcacgg cgaagggaat
      241 ctttaaatat ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc
      301 tgaccaggaa ggctggaacc ctgggttcac ggaaaaaatg gttggatggg caaaaaaaat
      361 ggagacaggt gaacgctctg tgattaaaaa tcctgaatac ttttcaacat atatgcagga
      421 agaactaaaa gcactggtct gagttaaatt tatatcagca taaatgggtc aggacgcttt
      481 taatcacata taaaaagcgt cctttttctc acaatcaaat gatcatcggc ttctataacg
      541 aaatcgatac ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattagctc
      601 gccggagtgg ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctactcat
      661 cccttgtgcc atcagttcct ggatccgctc gacagctttt tgctgctgtt catgggtgag
      721 tgaaggtaaa cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat
      781 gctacccggc acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat
      841 tactttaccc tggcgtcagg acgccgctga attttatttc tcccgcttaa gccacctgcc
      901 gtgggcgatg cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt
      961 ggtcgccgat ccgatttgca ctttaaccac tttaggtaaa gaaaccgttg ttagtgaaag
     1021 cgaaaaacgc acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg
     1081 cgcagacatt cgcccaacgc ataacgaaga tttgccattt cagggcggcg cactggggtt
     1141 gtttggctac gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat
     1201 cgttctgccg gatatggcag tgggtatcta cgattgggcg ctcattgtcg accaccagcg
     1261 tcatacagtt tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag
     1321 ccagcaattc tcgccgcagg aagatttcac gctcacttcc gactggcaat ccaatatgac
     1381 ccacgagcag tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg
     1441 ctatcaggtg aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc
     1501 attccttcag cttaatcagg ccaaccgcgc gccatttagc gcttttttac gtcttgaaca
     1561 gggtgcaatt ttaagccttt cgccagagcg gtttattctt tgtgataata gtgaaatcca
     1621 gacccgcccg attaaaggca cgctaccacg cctgcccgat cctcaggaag atagcaaaca
     1681 agcagaaaaa ctggcgaact cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt
     1741 aatgcgtaat gatatcggtc gtgttgccgt agccggttcg gtaaaagtac cagagctctt
     1801 cgtggtggaa cccttccctg ccgtgcatca tctggtcagc actataacgg cgcgactacc
     1861 agaacagtta cacgccagcg atctgctgcg cgcagctttt cctggtggct caataaccgg
     1921 ggctccgaaa gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgtaatgc
     1981 ctggtgcggc agcattggct atttgagctt ttgcggcaac atggatacca gcattactat
     2041 ccgcacgctg actgccatta acggacaaat atactgctct gcgggcggtg gaattgtcgc
     2101 cgatagccag gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg
     2161 ccaactggag aagtaaaacg tggaataccg tagcctgacg cttgatgatt ttttatctcg
     2221 ctttcaactt ttgcgcccgc aaattaaccg ggaaacccta aatcatcgtc aggctgccgt
     2281 gttaatcccc atcgtccgtc gaccacaacc ggggttgttg ctgactcagc gttcaattca
     2341 tctgcgtaaa cacactggac aagtggcatt ccctggaggt gcagtcgatg acacggacgc
     2401 atcagttatc gccgccgcgc tgcgcgaagc tgaagaagag gtcgctatac cgccttccgc
     2461 cgttgaagtt atcggcgtgc tgccgcccgt cgatagcgta actggctatc aggtaacccc
     2521 agtggtcggc attatcccgc ccgatctgcc gtatcgcgcc agtgaagatg aagtctcggc
     2581 ggtgtttgaa atgccgctcg cccaggcatt acatctgggt cgttatcacc ctttagatat
     2641 ctaccgccgt ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg
     2701 gggaatgacc gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga acccctgact
     2761 atacttatct ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat
     2821 cgcggttttt gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt
     2881 acactatgcg ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat
     2941 cgtgattagt ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt
     3001 agggcctatg aaggcgggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga
     3061 tagcgttact cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca
     3121 ccacaccgat atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat
     3181 cgacagtatt cccggtttta ttcgcgacgt aaaagagcgc gaacgtctgc tgctggcaca
     3241 gggacggcat gaagtggatt tcccgcgcga caacgggatg cgttttcata acggcaacct
     3301 gccgctgcat gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag
     3361 caaaacttat tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga
     3421 tgctgccaac gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta
     3481 ctgtaatgaa accggctatt cgctgtctgg tctcgctatg cagaacgaac tggcgctgca
     3541 cagcaagaaa gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat
     3601 cgatcgcggg atgaacaccg aaggtgtact gccaggcccg ctgcgcgtgc cacgtcgtgc
     3661 gtctgccctg cgccggatgt tagtttccag cgataaactg tctaacgatc cgatgaatgt
     3721 cattgactgg gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg
     3781 tgtggtaact gcgccaacca acggtgcctg cggtatcgtt ccggcagtgc tggcttacta
     3841 tgaccacttt attgaatcgg tcagcccaga catctatacc cgttacttta tggcagcggg
     3901 cgcgattggt gcattgtata aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca
     3961 gggcgaagtg ggtgttgcct gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg
     4021 tagcccggaa caggtttgcg tggcggcgga aattggcatg gaacacaacc ttggtttaac
     4081 ctgcgacccg gttgcagggc aggttcaggt gccgtgcatt gagcgtaatg ccattgcctc
     4141 tgtgaaggcg attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc
     4201 gctggataag gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ccaaataccg
     4261 cgaaacctca cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttactcgc
     4321 ccatctgcaa cggatgggcg aatttatacc cgctttctcg tctgctgtaa tattccccac
     4381 tacacttcca ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc
     4441 aaaaagcaca acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt
     4501 gcgcactcgt tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa
     4561 atcaacaacg ggtagtacaa ttcgccaatc acgctgtaga ggaattagat aaagtactgc
     4621 ttcctctaca ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg
     4681 cccatttgcc attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg
     4741 tgcaagacgg cacactttat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg
     4801 acattctggc tgaacttcct gcaccgcaac cacttttacg cctgacgatc gaccgtgccc
     4861 tgattaaagg cagtccggtt ttgattcaat ggacgcctgc agcaggcagt agcaatgctg
     4921 gggtcataga gatgattaac atcgacttac tgacggcaat gctccttgag ccacaactgc
     4981 cgcaaatcag tagcgccagc ctgacggtgg acaaacggca tttgctctat ggtaatgggc
     5041 tggtagattc ccttccgcaa cctgaagaca atgaaaacta tcaggtttct tcgcaaagct
     5101 ttccttttac cattaacgta aatggtccgg aggctacggc gctggcatgg cactatcttc
     5161 caacacaatt accgctggcg gtgctgctaa gtttactggt gggctatatc gcctggctgg
     5221 cgaccgctta ccggatgagc ttttcccgcg aaattaatct gggcctggcg caacatgagt
     5281 tcgaattgtt ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga
     5341 ttttgctgcg ctggaataat ccgcgtcagg gctggatttc accggatgtg tttattccta
     5401 tcgcggaaga acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc
     5461 gtcagcgcca tgttttcccg atgagtagtc agtttcatgt tggcattaac gtcgcaccca
     5521 gccattttcg ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc
     5581 cgattcagca actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattatc
     5641 ggattgcccg cgagctgcat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg
     5701 gcaacagctc gttttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt
     5761 catttaccgc agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg
     5821 cgctggggca aagactgaat attgaactgg tggcgga
//
LOCUS       DADWTH010000173         5797 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.177, whole genome shotgun sequence.
ACCESSION   DADWTH010000173 DADWTH010000000
VERSION     DADWTH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.177"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..201
                     /locus_tag="JNY02_003882"
                     /pseudo
     CDS             1..201
                     /locus_tag="JNY02_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            778..1906
                     /gene="ltrA"
                     /locus_tag="JNY02_003883"
                     /pseudo
     CDS             778..1906
                     /gene="ltrA"
                     /locus_tag="JNY02_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1989..2168
                     /locus_tag="JNY02_003884"
                     /pseudo
     CDS             1989..2168
                     /locus_tag="JNY02_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2198..2680)
                     /locus_tag="JNY02_003885"
                     /pseudo
     CDS             complement(2198..2680)
                     /locus_tag="JNY02_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2936..3916)
                     /locus_tag="JNY02_003886"
     CDS             complement(2936..3916)
                     /locus_tag="JNY02_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBB9435217.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3916..5115)
                     /locus_tag="JNY02_003887"
     CDS             complement(3916..5115)
                     /locus_tag="JNY02_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB9435218.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5437..>5797)
                     /locus_tag="JNY02_003888"
     CDS             complement(5437..>5797)
                     /locus_tag="JNY02_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBB9435219.1"
                     /translation="TFHPSLTQHWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTP
                     FCIGMVTSDDWGSYAREVPKEKHLTGKIFTQRIERNNRTLRTRIKRLARKTICFSRSV
                     EIHEKVIGSFIEKHMFY"
BASE COUNT         1584 a         1327 c         1388 g         1498 t
ORIGIN      
        1 actctccagg aaacccgggg cggttcagtt gctgaactgc tggaaccgct gtatgacatc
       61 ctgcgccagt atgtgctgat gcccggtaaa gtccatgccg atgatatccc cgttccggtc
      121 caggagccgg gcagcggtaa aacaaggact gcccggctgt gggtctacgt tcgtgatgac
      181 cgtaacgccg gttcggaaat tgcagcataa gttatccatg ctctgaggtg ggtggaggtg
      241 ggccctccgg tcagggttta caggagcact gaccaaacga cccggtgctg cttcattcta
      301 aggaatgagg tggtgagcga ctcgggaaaa ggcagagatc tactgtcacg tggggttcgg
      361 gaagctgaac gaaagtaaac tgtccgatga agcatcgaaa tcttagatga tgccaaaatg
      421 gggccgtaca gccgtcccct gataagcgca gaagacacct gcttactgac tgcgcggcat
      481 ccggtgtgaa ggcagcagga tcccgggcca gaccatctca gggaacgtga gaacctgttt
      541 ccggcacgta tcgggaaaca ggggcggacc cattcgtagt agtgatgaaa gatctgtcat
      601 gggtctggag cgaagggatg gggatcacat tcattgcaga agacacaacc caaaagggga
      661 tgacgtaact gatgcaacga aaatcatttg aaataccaaa agcactggtc tgggcatcat
      721 atctggatgt gcgtcgcaac aagggagccc cccggatgcg acgggcagac tttgaaaatg
      781 ttcgaccagc aacgtgatgg caatctatac aagatctgga accggctatg ttcgggaaca
      841 tggtttccgc caccagtgct ggaaaaacgg atcccgaagt cgaatggcaa ggaacgcatt
      901 ctggggatcc caacagtatc cgatcgaatt gctcagggag cgataaaact tttcatggaa
      961 gaaaagcttg acccgatttt ccatgctgat tcatatggtt accgaccagg caaatcagct
     1021 catgacgctc tgaaacaatg tgccatccgg tgctggcgtt atagctggat actggaagtg
     1081 gacatcagtg cgtttttcga ccatgtgaga catgatctgg tgctcaaagc tctggaacac
     1141 cacgggatgc ctaaatgggt catcctgtac tgtcgacgct ggatggaagc gccaatgcag
     1201 agttgtgaaa atggagaatt aataacccga acacgaggca ctccgcaggg cggggtcatt
     1261 agtccgctac ttgctaactt gttccttcac tatgcatttg atttgtggat ggaaagagaa
     1321 tatcgggggg taccgtttga gaggtacgct gacgatattg tagtacattg ttcacgaatg
     1381 agtgatgtaa caaggctaaa gaacaggtta tcggagcgct tctcggaagt cgggctggtc
     1441 ctgaatgcag ggaaaacgaa tatcgcctac attgacacgt ttaaaaggag aaacgtcgca
     1501 acgagtttca cctttctcgg atatgacttc aaagtgcgta cgctgaagaa tttcaaaggc
     1561 gaacggtacc gaaaatgcat gccgggtgcg tcaaatgcag caatgctgca aaataacaga
     1621 aacaatcaag aagtggcgta tacatcgctc aacagctgag agtttgctgg attttgcgag
     1681 gcgctacaat gcgatagtga gaggctggat cgagtactac ggaaagttct ggtccagaaa
     1741 tttcaactat cgactgtgga gtgcaatgca gtcacgtctg ctcaagtgga tgcagtctaa
     1801 atacagactt tcgaaccgga aggctcagcg aaagctgacg ctggtaagga aggagtatcc
     1861 gaagctattt gttcactggt atttactacg tgcatcaaat gagtgatcaa gagccgtatg
     1921 acgcgagagt gtcacgtacg gttccgtgag cagccgggga gtgaaattct ctccggctga
     1981 ctcgactcgc gggttccgac agcgatgacg aacatgcggc ggtgttgtac tcgctgatcg
     2041 gcacatgccg tctgaacaat gtggaaccag aaaaatggct gcgttacgtc attgagcata
     2101 tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctggaaagtt gatctgagct
     2161 ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat tctgctgcgc
     2221 ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt atcagttgtg
     2281 tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ctggacgtcc tgttgctcca
     2341 gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct attgcctgtg
     2401 cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt gccggaagca
     2461 atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat gccccgccag
     2521 cctccatcgc aacagtcatt ttaggctgtt gcacctgcca gttaagcagt tgattgcgag
     2581 aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga taaaagttcg
     2641 ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc ttacttttgt
     2701 ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg ggaagactat
     2761 ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa gaagatacgg
     2821 tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa aagcaaaatt
     2881 tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg aattttcaca
     2941 gcgaattcag attatctttt agtacaagag caatcatacg atctaactta tcctgaactt
     3001 ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt tttcgagcaa
     3061 atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc tttactcctg
     3121 aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt ttaacctcgt
     3181 cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata ttcttcacaa
     3241 cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc agttgcctgt
     3301 aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct ttaggtacgg
     3361 acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt tccgcaattt
     3421 gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt tcacgaatat
     3481 tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa acagtaagct
     3541 catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct gcccgacgac
     3601 gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat ggatagaatt
     3661 gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg gctgtctgtt
     3721 cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca tgatcaacct
     3781 tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga actggagccg
     3841 atatttcttc agtattattc aggatatttg tattcagcgt acggccaata gtcggtcggt
     3901 gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa aaactgcttt
     3961 agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc caacataagt
     4021 aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa aaccatcaag
     4081 acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca ggctatggca
     4141 gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg cctgcaaacg
     4201 gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag ccaaatattt
     4261 aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga tatctgcggc
     4321 agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa ggatgaagtc
     4381 gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt tgttttgtcc
     4441 taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt cgtcaataga
     4501 agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt ccagtaattc
     4561 ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca gccccaccgc
     4621 atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa ggagcagaat
     4681 acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta cggaaacact
     4741 actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat atgcctcact
     4801 ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa tgttctggat
     4861 ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat agccctgagc
     4921 ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa gcttagctac
     4981 agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct tttgctcctg
     5041 aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct tacttaactg
     5101 ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta caaaaaaaac
     5161 tgcattagca gggtaatgca tgcaagtaca tattgtacat gcttatatta aaaatgcgcg
     5221 cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg cctttccggg
     5281 agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg acaacagctt
     5341 tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg acggcgatga
     5401 cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt gcttttcaat
     5461 gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga ttgttttacg
     5521 tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgttcaatac gttgcgtgaa
     5581 gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc cccagtcatc
     5641 gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc gacaggtctc
     5701 atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     5761 ccagagccag tgttgtgtca acgacggatg aaaagtg
//
LOCUS       DADWTH010000174         5735 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.178, whole genome shotgun sequence.
ACCESSION   DADWTH010000174 DADWTH010000000
VERSION     DADWTH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.178"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            55..1515
                     /locus_tag="JNY02_003889"
     CDS             55..1515
                     /locus_tag="JNY02_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBB9435220.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVAEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="JNY02_003890"
     CDS             complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="JNY02_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBB9435221.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1932..2643)
                     /gene="ydfH"
                     /locus_tag="JNY02_003891"
                     /pseudo
     CDS             complement(1932..2643)
                     /gene="ydfH"
                     /locus_tag="JNY02_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
     gene            complement(2732..3478)
                     /gene="ydfG"
                     /locus_tag="JNY02_003892"
     CDS             complement(2732..3478)
                     /gene="ydfG"
                     /locus_tag="JNY02_003892"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBB9435222.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3615..5660
                     /gene="dcp"
                     /locus_tag="JNY02_003893"
     CDS             3615..5660
                     /gene="dcp"
                     /locus_tag="JNY02_003893"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBB9435223.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1435 a         1363 c         1482 g         1455 t
ORIGIN      
        1 tttattcata taacaaaaca tatagcttgc catatttaaa tttaaggaat tgttatggga
       61 aataatttgt tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca
      121 agaattgttc atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat
      181 attcttgcta cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc
      241 gaacagcaaa ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg
      301 gccgatgcgt ggacggctcg cgtcgttggc gtcgttaaaa aagccttgca cgtacagatg
      361 gatggcttag aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg
      421 acaatcaccg aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac
      481 ccgatggtcg ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt
      541 gttgaggcgc tggctcgccg taaagcggca ggacttcccg catttaccgt catgtcatgt
      601 gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta cgcgcaagcc
      661 gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg
      721 gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca acttaccggt
      781 gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat cgaagataac
      841 tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag tgatgtgctg
      901 ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcatatctg
      961 ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca ttatcgtcat
     1021 gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat
     1081 ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt acgtcatcga
     1141 acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt
     1201 cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg
     1261 atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag tgacccactg
     1321 ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca
     1381 ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt gtttactgca
     1441 aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa
     1501 tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac ttagttcatg
     1561 ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt
     1621 tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca
     1681 gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga
     1741 tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc acgtaacggg
     1801 cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt
     1861 ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg gatgggatga
     1921 gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat ctggcgcacg
     1981 gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt
     2041 ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg tggagaaaca
     2101 tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc aatggtatcc
     2161 cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc atcaagttca
     2221 aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc
     2281 agttggtagc actggctttc ggtaatcatg ctcgcactgg ctttcggtaa tcatgctcgc
     2341 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     2401 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     2461 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     2521 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     2581 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     2641 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     2701 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     2761 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     2821 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     2881 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     2941 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     3001 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     3061 gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     3121 ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca atcatcgttt
     3181 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     3241 ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc tcttcaatag
     3301 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta
     3361 actcctgcaa ccgctcctgg cgacgcccag tggcgataac tttatgccct tgttgaataa
     3421 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     3481 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     3541 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     3601 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     3661 tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     3721 tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     3781 acaatactat tctggcactg gaacaaagcg gagaattaat tacccgcgtt accagcgtct
     3841 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     3901 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     3961 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     4021 tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa gctgataaag
     4081 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcggt
     4141 tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     4201 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     4261 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc
     4321 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     4381 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     4441 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     4501 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat
     4561 tagcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg cagccgtggg
     4621 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     4681 tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg accgcgaatc
     4741 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc
     4801 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     4861 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     4921 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc gctgccggtg
     4981 agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt ggtcatacgc
     5041 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     5101 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     5161 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     5221 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     5281 ttctcgatat gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt
     5341 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     5401 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     5461 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     5521 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     5581 tggaacgcct gcatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac
     5641 atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     5701 ctggttatag cagtaattgc agcatgttac gacaa
//
LOCUS       DADWTH010000175         5630 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.180, whole genome shotgun sequence.
ACCESSION   DADWTH010000175 DADWTH010000000
VERSION     DADWTH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.180"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            34..435
                     /locus_tag="JNY02_003894"
     CDS             34..435
                     /locus_tag="JNY02_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBB9435224.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JNY02_003895"
     CDS             447..818
                     /locus_tag="JNY02_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435225.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="JNY02_003896"
     CDS             805..1395
                     /locus_tag="JNY02_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435226.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="JNY02_003897"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="JNY02_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="JNY02_003898"
     CDS             1802..2542
                     /locus_tag="JNY02_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBB9435227.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="JNY02_003899"
     CDS             2601..2987
                     /locus_tag="JNY02_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBB9435228.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="JNY02_003900"
     CDS             2996..3325
                     /locus_tag="JNY02_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBB9435229.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..5630
                     /locus_tag="JNY02_003901"
                     /pseudo
     CDS             3297..5630
                     /locus_tag="JNY02_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT         1387 a         1306 c         1784 g         1153 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgctca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggta agcagtgctg
     3961 cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga
     4021 aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt
     4081 ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac
     4141 tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc
     4201 tgggtctggg taaagcctgg catgacggcg gaaggagggc gaggaattta accgtcagct
     4261 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
     4321 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
     4381 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
     4441 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
     4501 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgactg ccactcagct
     4561 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
     4621 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
     4681 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
     4741 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
     4801 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
     4861 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
     4921 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
     4981 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
     5041 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
     5101 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
     5161 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
     5221 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
     5281 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
     5341 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
     5401 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
     5461 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
     5521 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
     5581 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc
//
LOCUS       DADWTH010000176         5599 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.181, whole genome shotgun sequence.
ACCESSION   DADWTH010000176 DADWTH010000000
VERSION     DADWTH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.181"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..410
                     /locus_tag="JNY02_003902"
     CDS             <1..410
                     /locus_tag="JNY02_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435230.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="JNY02_003903"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="JNY02_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNY02_003904"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNY02_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HBB9435231.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSIVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1379 a         1402 c         1329 g         1489 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actatacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DADWTH010000177         5597 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.182, whole genome shotgun sequence.
ACCESSION   DADWTH010000177 DADWTH010000000
VERSION     DADWTH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.182"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            47..682
                     /locus_tag="JNY02_003905"
                     /pseudo
     CDS             47..682
                     /locus_tag="JNY02_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
     gene            complement(736..1341)
                     /gene="azoR"
                     /locus_tag="JNY02_003906"
     CDS             complement(736..1341)
                     /gene="azoR"
                     /locus_tag="JNY02_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBB9435232.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1542..5444
                     /gene="hrpA"
                     /locus_tag="JNY02_003907"
     CDS             1542..5444
                     /gene="hrpA"
                     /locus_tag="JNY02_003907"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBB9435233.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1385 a         1369 c         1553 g         1290 t
ORIGIN      
        1 tatttcagga aggagtgtcc gttaaactca ggctacctca gtcgtaggcg cgtgcattgt
       61 gttgatggag ctaatgatga tggttcagtt atggtggtca gtctggttat gttggccagt
      121 attatcgcta ctcattgttg gccgtgggtg cggtgggctt ggcgcgataa caacagggaa
      181 agatagtcag gggaaactcc cgcccgccgt ttactggctg acattgccct ggcgcatcgg
      241 gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag ccggtgagca aaatgactgc
      301 tggtgtttat ttaagggcgt ttccacgaca tattccggca cagaatgcgg ttctggacgt
      361 cacctttgaa ttccctcgtg gacgagcgac aaaagatcga ctctatttct gtgtaccgat
      421 gttggatctg gtggttccgg aagaggggga gctccgacag gccgtggcga tgctggaaac
      481 attacgcgaa gagcaaggca gcgttctggt ccattgcgca ttgggattat cgcgcagtgc
      541 gctggtagtg gcggcatggc tgttatgtta cggacactgt aaaacagttg atgaagcgat
      601 tagctttatt cgagccagac gctcgcatat tgtgcttaag gaagagcaca aagcgatgtt
      661 gaaattatgg gaaaacaggt aagtggattg agatgtggac tgaatatcta cagtccacat
      721 caagaccgtg tccggttatg cagcaacaat gctgtcgatg gctgcttttg cgtcagactg
      781 tgctttcgct gccatttccg gaccgtatgc gatcccttcg gcgaagacaa atttcacatc
      841 ggtaatgccg ataaagccga ggaacgtgga cagatacggc gtcaccaggt ccgttggtcc
      901 atctttgtgg atcccaccgc ggctggtaat aacgatggct tttttacccg ttaccagacc
      961 ttccggaccg ttctcggtat agcggaaagt aacgcctgcg cgtgcaacca ggtcaaaata
     1021 gtttttcaac tgagttgaga tgttgaagtt atacatcggt gccgcaataa cgataacgtc
     1081 gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct gctgacgcgg
     1141 agtcagcggc gcatcgctcg gacgcagagc gccaaccagt tcgccatcca gtaccggaat
     1201 cggatttgca gctaggtcgc gaacggtgat ttcatcagcg gagtgctttt cgcgccattg
     1261 ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga tgctggattt
     1321 aagaactaat accttgctca tggtgtttcc ttatagatgt ttgaatggac gatgccccgt
     1381 tgcttgctga cactttattc acaatcctgc cacagagata gcgcaataaa tcgaagtcta
     1441 tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat atctatcatt
     1501 taaaagaaaa ttaatcaggc agactgctgc ccactaacgt tatgacagaa caacaaaaat
     1561 tgacctttac tgccttgcaa cagcggctgg attcgctgat gctgcgcgac agactgcgtt
     1621 tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc tgatgcacaa caggccattt
     1681 tccaggagat ggcgaaagag attgaccagg cggcagggaa agtcctgctg cgtgaagcgg
     1741 cacgaccgga aatcacttat cctgacaatt taccggttag tcagaaaaaa caggacatac
     1801 tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg ggaaaccggc tccggtaaaa
     1861 cgactcagct accgaaaatc tgtatggagc tggggcgcgg tattaaaggg ctgatcggtc
     1921 atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa ccgtattgcg gaagagctga
     1981 aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac gtaagtgata
     2041 acacgatggt caagctgatg accgacggga tcctgctggc ggagatccag caagaccgcc
     2101 tgctgatgca gtacgacacc atcattattg acgaagcgca cgaacgcagc ctgaatatcg
     2161 attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta aaaatcatta
     2221 tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat gcgccgatta
     2281 ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgcccgatt gttgaagaag
     2341 ccgatgacac cgagcgcgat cagttgcagg cgatttttga cgccgtagac gaactaagcc
     2401 aggaaagccc cggcgacatt ctgatcttta tgagcggcga gcgggaaatt cgcgataccg
     2461 ccgatgcgct gaacaagctg aacttacgcc ataccgaaat cttgccgctt tatgcgcggc
     2521 tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag cggacggtgc attgtgctgg
     2581 cgaccaacgt cgcggaaacc tcgctgaccg taccggggat taaatacgtc atcgaccccg
     2641 gtacagcgcg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg ccgattgaac
     2701 ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg tggtcgtgtg tccgaaggga
     2761 tctgtattcg tctctattcc gaagacgatt tcctctcgcg cccggagttt accgatccgg
     2821 agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcgctg gggctgggcg
     2881 atatcgctgc gttcccgttt gtcgaagcac cggataaacg caatatccag gacggcgtgc
     2941 gtctgctcga agaactgggc gcgatcacca cggatgaaca ggccagcgcc tataaactga
     3001 cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc acgtctggcg cgtatggtgc
     3061 tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc gcgctctcca
     3121 tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc atcggacgaa aaacatcgtc
     3181 gcttccacga caaagaatcc gacttcctcg cgtttgtaaa tctgtggaat tatcttggcg
     3241 agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct gtgtcgtacc gattacctca
     3301 actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag gtggtgaaag
     3361 aacttggcat tccggtcaac agcgaaccgg cggagtatcg tgaaattcac atcgccttac
     3421 tgaccggttt gctttcccat atcggcatga aagatgccga taaacaggaa tataccggtg
     3481 cacgtaacgc gcgtttctcc atcttccccg gttctggttt attcaaaaag ccgccgaaat
     3541 gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt gctgcgcgta
     3601 tcgacccgga atgggtagaa cccgttgctc agcatttgat taaacgcacc tacagcgaac
     3661 cgcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact gtttatggtt
     3721 tgccgattgt tgctgcgcgc aaggtcaact acagccagat cgattcggcg ttatgtcgtg
     3781 aactctttat tcgccacgct ctggtggaag gtgactggca gacgcgtcac gcattcttcc
     3841 gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga acacaaatca cgtcgccgcg
     3901 atattctggt tgatgacgaa acgctgtttg agttctacga ccagcgcatc agccacgatg
     3961 taatctccgc tcgtcacttc gatagctggt ggaaaaaagt cagccgcgaa acgcctgatt
     4021 tgctcaactt tgaaaagagc atgttgatca aagaaggggc ggaaaaaatc agcaagctgg
     4081 attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagctat cagtttgagc
     4141 caggcgcgga cgcggacggt gtgaccgtac atattccgct gccgttactt aaccaggttg
     4201 aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg cgaattggtg attgctctga
     4261 ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc tgcaccaaac tacgccgaag
     4321 cgtttttagg ccgcgtcaca ccgctggagt taccgttgct cgacagcctt gagcgcgagt
     4381 tacggcggat gaccggcgtt accgttgacc gcgaagactg gcactgggat caggtgcccg
     4441 atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa caagaagcta aaagaagggc
     4501 gctcgctaca agatctgaaa gatgcgctga aaggcaaagt gcaggaaacg ctgtccgcgg
     4561 tggcggatga cggtatcgag cagagcggct tacatatctg gagttttggt cagctgccgg
     4621 aaagttacga acagaagcgt ggcaactata aagtgaaggc gtggccggcg ctggtggatg
     4681 aacgcgacag cgtggcgatc aaactgtttg ataacccgct ggagcaaaaa caggcaatgt
     4741 ggaatggtct tcgccgtcta ctgctgctaa atatttcctc gccgatcaag tatttacatg
     4801 agaagttacc aaacaaagcc aagctggggc tgtactttaa cccgtatggc aaagtgctgg
     4861 agctgatcga cgactgtatc tcctgcggtg tggatcaact gatcgacgcc aatgatggcc
     4921 cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtgcgcgcc gaactgaatg
     4981 acacggtggt ggatattgcg aagcaggtcg agcaaatcct cactgcggtg ttcaatatca
     5041 acaaacgtct gaaagggcgg gtggatatga ccatggcact ggggctttct gacattaaag
     5101 cgcagatggg cgggttggta tatcgcggtt ttgtcactgg taacggcttc aaacggctgg
     5161 gcgacacgct gcgatatttg caggcgattg aaaaacgact ggaaaaactg gcggttgatc
     5221 cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt ccagcaggcg tggcagcaat
     5281 ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga cgtgaaagag atccgttgga
     5341 tgatagaaga gctgcgcgtt agttacttcg ctcaacaact tggtacgcct tatccgattt
     5401 cagataagcg tattttgcag gcgatggagc agattagcgg ttaaccctgc tatttgccta
     5461 ataaatctaa aacccggtaa gcatttagcg ccgggttttt tattaattct aaaacggtaa
     5521 ggacaaaaat tcaggaattc agaaaaatac aattctcagc tgcaagatga ataatgttta
     5581 tctacagcat ttcctta
//
LOCUS       DADWTH010000178         5583 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.183, whole genome shotgun sequence.
ACCESSION   DADWTH010000178 DADWTH010000000
VERSION     DADWTH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.183"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            29..244
                     /locus_tag="JNY02_003908"
     CDS             29..244
                     /locus_tag="JNY02_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBB9435234.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="JNY02_003909"
     CDS             237..623
                     /locus_tag="JNY02_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBB9435235.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="JNY02_003910"
     CDS             631..1317
                     /locus_tag="JNY02_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB9435236.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="JNY02_003911"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="JNY02_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBB9435237.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="JNY02_003912"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="JNY02_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBB9435238.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="JNY02_003913"
     CDS             complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="JNY02_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBB9435239.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4044..4415
                     /locus_tag="JNY02_003914"
     CDS             4044..4415
                     /locus_tag="JNY02_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9435240.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4425..>5583)
                     /locus_tag="JNY02_003915"
     CDS             complement(4425..>5583)
                     /locus_tag="JNY02_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBB9435241.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1543 a         1030 c         1215 g         1795 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat aaggttttta
     3601 ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc agaccaacca
     3661 tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg ctgcttataa
     3721 caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc tttggtcacc
     3781 aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc aacaaacaat
     3841 attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt
     3901 ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga
     3961 tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc
     4021 gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa
     4081 cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg
     4141 atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaaag
     4201 taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa gcggctgttg
     4261 agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa tttgattccc
     4321 aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt tattatcggc
     4381 gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa tttccccaaa
     4441 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
     4501 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
     4561 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
     4621 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
     4681 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
     4741 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
     4801 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
     4861 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
     4921 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
     4981 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
     5041 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
     5101 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
     5161 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
     5221 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
     5281 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
     5341 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
     5401 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
     5461 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
     5521 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
     5581 aat
//
LOCUS       DADWTH010000179         5576 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.184, whole genome shotgun sequence.
ACCESSION   DADWTH010000179 DADWTH010000000
VERSION     DADWTH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.184"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..87)
                     /locus_tag="JNY02_003916"
     CDS             complement(<1..87)
                     /locus_tag="JNY02_003916"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBB9435242.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNY02_003917"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNY02_003917"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HBB9435243.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSSPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGEGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="JNY02_003918"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="JNY02_003918"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HBB9435244.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1730 a         1256 c          935 g         1655 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctctc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact gaaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggctg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gagggggaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aattttatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccggaaatc ctggagacta aactccctga gaaagaggta
     5461 aacaggatga ctaaaaatac acgtttttcc cctgaagtcc gtcaacgggc agttcgtatg
     5521 gttctggaaa gtcagggcga atataactca caatgggcag cgatttgttc cattgc
//
LOCUS       DADWTH010000180         5537 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.185, whole genome shotgun sequence.
ACCESSION   DADWTH010000180 DADWTH010000000
VERSION     DADWTH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.185"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JNY02_003919"
                     /pseudo
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JNY02_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JNY02_003920"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JNY02_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBB9435245.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5537)
                     /gene="mdtF"
                     /locus_tag="JNY02_003921"
     CDS             complement(2659..>5537)
                     /gene="mdtF"
                     /locus_tag="JNY02_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HBB9435246.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGISYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1463 a         1286 c         1302 g         1486 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgtag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gatgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catagcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtcttttata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaag
//
LOCUS       DADWTH010000181         5531 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.186, whole genome shotgun sequence.
ACCESSION   DADWTH010000181 DADWTH010000000
VERSION     DADWTH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.186"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            100..1008
                     /locus_tag="JNY02_003922"
     CDS             100..1008
                     /locus_tag="JNY02_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBB9435247.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNY02_003923"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNY02_003923"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBB9435248.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /gene="loiP"
                     /locus_tag="JNY02_003924"
                     /gene_synonym="yggG"
     CDS             3323..4081
                     /gene="loiP"
                     /locus_tag="JNY02_003924"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBB9435249.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNY02_003925"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNY02_003925"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBB9435250.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="JNY02_003926"
     CDS             complement(5343..>5531)
                     /locus_tag="JNY02_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435251.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1323 a         1484 c         1478 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tccacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag ccaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gataccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DADWTH010000182         5392 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.187, whole genome shotgun sequence.
ACCESSION   DADWTH010000182 DADWTH010000000
VERSION     DADWTH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.187"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..446)
                     /locus_tag="JNY02_003927"
     CDS             complement(<1..446)
                     /locus_tag="JNY02_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBB9435252.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="JNY02_003928"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="JNY02_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBB9435253.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALSAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="JNY02_003929"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="JNY02_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBB9435254.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JNY02_003930"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JNY02_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBB9435255.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="JNY02_003931"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="JNY02_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBB9435256.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFFFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..5389)
                     /locus_tag="JNY02_003932"
     CDS             complement(5216..5389)
                     /locus_tag="JNY02_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rtn protein"
                     /protein_id="HBB9435257.1"
                     /translation="METQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPA
                     RHNGHITPIMSLR"
BASE COUNT         1311 a         1362 c         1373 g         1346 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcact cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttcttcttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg cc
//
LOCUS       DADWTH010000183         5337 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.188, whole genome shotgun sequence.
ACCESSION   DADWTH010000183 DADWTH010000000
VERSION     DADWTH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.188"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNY02_003933"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNY02_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBB9435258.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JNY02_003934"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JNY02_003934"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBB9435259.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JNY02_003935"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JNY02_003935"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBB9435260.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JNY02_003936"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JNY02_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBB9435261.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1456 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggt
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADWTH010000184         5294 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.189, whole genome shotgun sequence.
ACCESSION   DADWTH010000184 DADWTH010000000
VERSION     DADWTH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.189"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..583
                     /locus_tag="JNY02_003937"
                     /pseudo
     CDS             1..583
                     /locus_tag="JNY02_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            647..1573
                     /gene="glsB"
                     /locus_tag="JNY02_003938"
     CDS             647..1573
                     /gene="glsB"
                     /locus_tag="JNY02_003938"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBB9435262.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1573..1932
                     /locus_tag="JNY02_003939"
     CDS             1573..1932
                     /locus_tag="JNY02_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBB9435263.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2071..3489
                     /locus_tag="JNY02_003940"
     CDS             2071..3489
                     /locus_tag="JNY02_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBB9435264.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3716..5167
                     /gene="uxaB"
                     /locus_tag="JNY02_003941"
     CDS             3716..5167
                     /gene="uxaB"
                     /locus_tag="JNY02_003941"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBB9435265.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1224 a         1234 c         1422 g         1414 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gcactgaaaa aatgcgtact actatccgcc
      241 aacggttctg gcgaatgtta ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc
      301 cgttgctgca atcaccattg cgaaagatgc agaacatgcg ctggaactgg ctaatgatag
      361 tgagttcggc ctttcagcga ccatttttac caccgacgaa acacaggcca gacagatggc
      421 ggcacgtctg gaatgcggtg gggtgtttat caatggttat tgtgccagcg acgcgcgagt
      481 ggcctttggt ggcgtgaaaa agagtggctt tggtcgtgag ctttcccatt tcggcttaca
      541 cgaattctgt aatatccaga cagtgtggaa agaccggatc tgacgcgaca tattcagctc
      601 tgatatactc gcaggtcttt tcagacctgc ggtccaggag tagaaagtgg cagtcgccat
      661 ggataatgca attttagaaa acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa
      721 agtcgcggat tatattccgg cgctggctac agtagacggt tcccgattgg ggattgctat
      781 ctgtaccgtt gacggacagc tttttcaggc cggagacgcg caagaacgtt tttccattca
      841 gtctatttcc aaagtgctga gtctcgttgt cgccatgcgt cattactccg aagaggaaat
      901 ctggcaacgc gtcggcaaag atccgtctgg atcaccgttc aattctttag tgcaactgga
      961 aatggaacag ggtattccgc gtaatccgtt cattaatgcc ggtgcgctgg tggtctgcga
     1021 tatgttgcaa gggcgactaa gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt
     1081 aagcggtgtg tctgatattt catacgatac ggtggtagcg cgttccgaat ttgaacattc
     1141 cgcgcgaaat gcggctatcg cttggctgat gaagtcgttt ggcaatttcc atcatgacgt
     1201 gacaaccgtt ctgcaaaact actttcatta ctgcgctctg aaaatgagct gtgtagagct
     1261 ggcccggacg tttgtctttc tggctaatca ggggaaagct attcatattg atgaacctgt
     1321 ggtgacgcca atgcaggcgc ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca
     1381 gaacgcgggg gagtttgcct ggcgggtggg actaccggcg aaatctggcg ttggtggcgg
     1441 tattgtggcg attgttccgc atgaaatggc catcgcagtc tggagtccgg aactggatga
     1501 tgcaggtaac tcgcttgcgg gtatcgccgt ccttgaacaa ttgacgaaac agttagggcg
     1561 ttcggtttat taatgcagtc tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt
     1621 cgctctcgct ttcgtctggg catgaaagag cgtcagtatt gcctggagaa aggcgcgcca
     1681 gtcatcgaac aacatgcggc ggattttgtc gctaaacgtc ttgctccggc attaccggct
     1741 aatgacggta agcaaccccc catgcgcggg catccggtgt ttatcgctca gcacgccacc
     1801 gcgacctgct gtcgcggttg tctggctaag tggcataaca ttccgcaggg tgtttcgtta
     1861 agcgaagaac aacaacgtta tatcgtggcg gtaatctatc actggctggt tgtacagatg
     1921 aatcagccat gagaattgcg gaaaacgata aaatagcatt cccagggtac ctttatttgc
     1981 cgccgctgtt agcgaacatg ctatccttta tgagaattta cttatcacgt tgagataatt
     2041 gccacccgca attgtatgga cagataatta atgcatgtac accccatttc aactttccgt
     2101 ttgttccagg aaggtcatct gctacgtaat agcatcgcta tttttgcgct aaccacgctg
     2161 ttctatttta ttggtgcaga gttacggctg gttcacgaac tttctctttt ctggccgctg
     2221 aatggcgtaa tggcgggggt gtttgcccgc tatgtctggc ttaatcgact gcattactat
     2281 gcgatcagtt atgtggcgat gctggtttat gatgccataa ccaccgaatg gggactggtt
     2341 tcactggtta tcaatttctc caatatgatg tttattgtta ccgtcgcctt actggtcgtg
     2401 cgggataagc gtctggggaa aaataagtat gagccagtaa gtgccttacg gctatttaat
     2461 tactgtctga ttgccgcatt attatgcgct attgtcgggg cgattggttc ggtcagtatt
     2521 gatagtctgg atttctggcc tttgcttgcg gactggttca gtgagcaatt ctcaacgggc
     2581 gtgttgatcg tgccttgtat gctgacgttg gctattcctg gagtgctgcc gcgctttaaa
     2641 gcagaacaga tgatgcccgc catcgcgctt attgtgtcgg ttattgcctc ggtagtcatt
     2701 ggcggagcgg ggagtctggc gttcccgctg cctgcattaa tctggtgtgc agtgcgctat
     2761 acgccgcagg taacatgtct gttgaccttt gtcaccggtg cggtggaaat cgtactggtg
     2821 gcaaattcgg tgattgatat ctcggtcggt tcgccgttct ccattccgga aatgttctcc
     2881 gcacgtctcg gtattgccac gatggcgata tgcccaatta tggtttcttt tagcgtggca
     2941 gcgatcaatt tgctaatgaa gcaagttgcg ctgcgagccg actttgattt tctgactcag
     3001 gtttactcac ggtccggtct ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa
     3061 catctgacgg tcatgctgct tgatatcgac tatttcaaaa gcattaacga taactatgga
     3121 catgaatgtg gcgataaagt gttaagcgtg tttgcccggc atattcagaa gattgtcggt
     3181 gataaggggc tggtggcgcg catgggcggc gaagaatttg ctgttgcagt gccgtcggtg
     3241 aatcctgtcg atggtctgct aatggcggaa aaaatccgta aaggcgttga actgcaacca
     3301 ttcacctggc aacaaaaaac gctttatctc acggtaagta ttggcgtcgg tagtggtcgc
     3361 gcatcgtacc gaacgctgac cgatgatttt aataaattga tggtcgaagc cgatacgtgt
     3421 ctgtatcgct cgaagaaaga tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa
     3481 gttgtctgac atctttattc attccgcgcg atttgaatgc aatgtgttct accactttat
     3541 gatccaggaa aaggcgtatt tcctcgatta aaccatgatc cgcgccacac ttttctcata
     3601 aaactgctca ttaatcatca ggagttgatc aaaacagggg ttcggcgtat taaagtttgc
     3661 taacctgtcg caccaattca aacaggttgt atgactaatc agaagggaac ccattgtgaa
     3721 aacactaaat cgtcgcgatt ttcccggtgc acagtatcca gaacgtatca ttcagtttgg
     3781 tgaaggtaac ttcctgcgcg cctttgttga ctggcaaatc gatctcctga atgagcacac
     3841 cgatctgaat tctggcgtgg tcgttgttcg tccgattgaa acttcattcc cgccgtcact
     3901 gagcacgcag gatggtctgt acaccaccat tatccgtggc ctgaatgaga agggggaagc
     3961 ggtcagcgac gcgcgtctga ttcgctcggt taatcgtgaa atcagcgtct acagtgaata
     4021 cgatgaattc ctgaaactgg cgcacaaccc ggaaatgcgc tttgttttct ctaacaccac
     4081 cgaagcgggt atcagctacc atgcgggcga taaattcgat gacgcgccag cggtaagcta
     4141 tccggcaaaa ctgacccgtc tgctgttcga acgctttagc catttcaacg gtgcgctgga
     4201 taaaggttgg atcatcattc cgtgtgagtt gattgactac aatggcgacg cgttgcgtga
     4261 actggtgctg cgctatgcgc aagagtgggc actgccagaa gcatttattc aatggctgga
     4321 tcaggctaac agcttctgtt ctacgctggt ggaccgtatc gttaccggtt atccgcgcga
     4381 tgaagtggct aaactggaag aagaactggg ttatcacgat ggtttcctcg acaccgctga
     4441 acacttttac ctgtttgtga ttcaggggcc gaaatcctta gcgactgagc tgcgcctgga
     4501 caaatatccg ctcaacgtgc tgattgttga cgatattaaa ccgtataaag aacgcaaagt
     4561 ggcaatcctc aacggtgcgc acaccgcgct ggtgccggtg gcgttccagg cagggctgga
     4621 taccgtaggt gaagcgatga acgatgctga aatttgcgca ttcgttgaaa aagccatcta
     4681 cgaagaaatt attccggtac tggatttgcc tcgtgatgaa ctggaatctt tcgccagtgc
     4741 agtcaccggg cgtttccgta acccgtacat taagcatcag ctgctgtcta tcgcgctcaa
     4801 cggtatgacc aagttccgca cccgcatcct gccgcagctg ctggcagggc agaaggcaaa
     4861 cggcacactt ccggcgcgcc ttactttcgc attagcggca ttgattgcgt tctatcgtgg
     4921 tgagcgtaac ggagaaacat atccggtaca ggacgatgca cactggctgg aacgttatca
     4981 gcaactctgg agccagcatc gtgaccgtgt tatcggtacc caggaactgg tagcgattgt
     5041 gttggcagaa aaagaccact gggagcagga cctgacgcaa gtgcctggtc tggtcgagca
     5101 ggttgctaat gacctggatg cgattcttga aaaagggatg cgcgaagctg tacgtccgtt
     5161 gtgctaataa caccgttact tctgaacccg gtttcgaccg ggtttcttat ttcactatat
     5221 tagttacaac atcctatatg cctttatcat taacaggtgt cacatgcagc gcctgttaat
     5281 gttgtaagct tgac
//
LOCUS       DADWTH010000185         5265 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.190, whole genome shotgun sequence.
ACCESSION   DADWTH010000185 DADWTH010000000
VERSION     DADWTH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.190"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..166
                     /locus_tag="JNY02_003942"
     CDS             <1..166
                     /locus_tag="JNY02_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBB9435266.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            349..2529
                     /locus_tag="JNY02_003943"
     CDS             349..2529
                     /locus_tag="JNY02_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBB9435267.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2670..3953)
                     /locus_tag="JNY02_003944"
     CDS             complement(2670..3953)
                     /locus_tag="JNY02_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBB9435268.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4088..5158)
                     /locus_tag="JNY02_003945"
     CDS             complement(4088..5158)
                     /locus_tag="JNY02_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB9435269.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKIFASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5136..>5265)
                     /locus_tag="JNY02_003946"
     CDS             complement(5136..>5265)
                     /locus_tag="JNY02_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBB9435270.1"
                     /translation="FPPPVKDGREYLFHESAVKVDLNRPVTGSLLKRIRNGKKAKS"
BASE COUNT         1253 a         1290 c         1331 g         1391 t
ORIGIN      
        1 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
       61 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct
      121 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
      181 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
      241 cctggggatt cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt
      301 tattatcttt ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta
      361 tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc
      421 ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct
      481 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
      541 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg
      601 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
      661 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
      721 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggctggcgg gggcaaaatg
      781 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
      841 ggtggcggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc
      901 gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg
      961 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
     1021 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
     1081 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
     1141 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
     1201 gatggctgca tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac
     1261 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
     1321 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
     1381 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
     1441 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     1501 ttctcgctgg cggtttaccc gtcatcacag ccggtgttta tggatctagc caaaaaaggt
     1561 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc
     1621 tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac
     1681 ttcccgaacc gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgctgatg
     1741 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     1801 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     1861 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     1921 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     1981 atcctcgacg aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     2041 cgttcaaatc cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     2101 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     2161 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     2221 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     2281 gtcatcaact gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg
     2341 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     2401 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg
     2461 acggctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccat
     2521 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     2581 gctggcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     2641 cgccgcatcc ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg
     2701 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     2761 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     2821 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     2881 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
     2941 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
     3001 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
     3061 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
     3121 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
     3181 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg
     3241 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacact atcgcccagc
     3301 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
     3361 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
     3421 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
     3481 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
     3541 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc
     3601 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
     3661 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
     3721 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
     3781 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
     3841 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
     3901 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     3961 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     4021 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
     4081 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
     4141 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
     4201 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
     4261 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
     4321 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
     4381 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     4441 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     4501 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     4561 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     4621 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
     4681 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
     4741 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
     4801 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
     4861 aatttatgag tgtcttctgc ttgattcctc tgctggcgaa gattttttcg tagcgatcaa
     4921 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
     4981 tgtgtcctga aaataactca atgttggcct gtatagcttc agtgattgcg attcgcctgt
     5041 ctcggcctaa tccaaactct ttacccgtcc ttgggtccct gtagcagtaa tatccattgt
     5101 ttcttatata aaggttaggg ggtaaatccc ggcgctcatg acttcgcctt cttcccattt
     5161 ctgatcctct tcaaaaggct acctgttact ggtcgattta agtcaacctt taccgctgat
     5221 tcgtggaaca aatactctct tccatcctta accggaggag ggaat
//
LOCUS       DADWTH010000186         5257 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.191, whole genome shotgun sequence.
ACCESSION   DADWTH010000186 DADWTH010000000
VERSION     DADWTH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.191"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..346
                     /locus_tag="JNY02_003947"
                     /pseudo
     CDS             71..346
                     /locus_tag="JNY02_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
     gene            408..1172
                     /locus_tag="JNY02_003948"
     CDS             408..1172
                     /locus_tag="JNY02_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBB9435271.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(1665..2249)
                     /locus_tag="JNY02_003949"
     CDS             complement(1665..2249)
                     /locus_tag="JNY02_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB9435272.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(2249..3487)
                     /locus_tag="JNY02_003950"
     CDS             complement(2249..3487)
                     /locus_tag="JNY02_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9435273.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(3484..4389)
                     /locus_tag="JNY02_003951"
     CDS             complement(3484..4389)
                     /locus_tag="JNY02_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBB9435274.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(4511..5215)
                     /locus_tag="JNY02_003952"
     CDS             complement(4511..5215)
                     /locus_tag="JNY02_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBB9435275.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT         1088 a         1596 c         1592 g          981 t
ORIGIN      
        1 gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg cctcatcgct aactttgcaa
       61 cagtgccctt atttttctgc ggcttggatt gacatctttt ggcggcccca ttgcgcactt
      121 gggctacttc cgcgccgaat ttgtcacacg gcggcgctgg ctctccgaac ggagctatgc
      181 tgacttggtc gcgctttgtc agttcttgcc agggcctgca agcagccagg tcggcatagc
      241 ggtaggactg tctcgggctg gatacagcgg ggcgctggct gcttgggctg gcttcacgct
      301 gccgtctgcc atagccttga tcctttttgc gctcggcatc tccagcggct ctgttgcaaa
      361 aatcgtgaag cttgagcatg cttggcggag attggacgga cggaacgatg acggatttca
      421 agtggcgcca tttccagggt gatgtgatcc tgtgggcggt gcgctggtat tgtcgctatc
      481 cgatcagcta tcgcgacctt gaggaaatgc tggcggaacg cggcatttcg gtcgaccata
      541 cgacgatcta tcgctgggtc cagtgctacg ccccggagat ggagaagcgg ctgcgctggt
      601 tctggcggcg tggctttgat ccgagctggc gcctggatga aacctacgtc aaggtgcggg
      661 gcaagtggac ctacctgtac cgggcagtcg acaagcgggg cgacacgatc gatttctacc
      721 tgtcgccgac ccgcagcgcc aaggcagcga agcggttcct gggcaaggcc ctgcgaggcc
      781 tgaagcactg ggaaaagcct gccacgctca ataccgacaa agcgccgagc tatggtgcag
      841 cgatcaccga attgaagcgc gaaggaaagc tggaccggga gacggcccac cggcaggtga
      901 agtatctcaa taacgtgatc gaggccgatc acggaaagct caagatactg atcaagccgg
      961 tgcgcggttt caaatcgatc cccacggcct atgccacgat caagggattc gaagtcatgc
     1021 gagccctgcg caaaggacag gctcgcccct ggtgcctgca gcccggcatc aggggcgagg
     1081 tgcgccttgt ggagagagct tttggcattg ggccctcggc gctgacggag gccatgggca
     1141 tgctcaacca ccatttcgca gcagccgcct gatcggcgca gagcgacagc ctacctctga
     1201 ctgccgccaa tctttgcaac agagcccgcc gtgctagtct gctcggtgat ggtggagtga
     1261 agccaacccg caatcgggtt atgaatctgc atcgcgattc gcaatcagct gtctcttgag
     1321 catgtcgaac tcctgcgatg ttatctcgcc gtgctcgtgc tgctacacca acttgtccaa
     1381 ctcatccgcc acaccgctcc ccaccacctg cgatggccag gttgtttgct gggcgcattg
     1441 gcggagtatc gtctcgaccc gggacaccca caccggcaca accttacggg agtgattcac
     1501 tgtcaaagaa tcggcccggt gctctgacgc aagtatcggg atggtcacca tttgtaagcc
     1561 gtagacctga gtggtgatca agacttcgat accaccgacc gtaccggtac taatcgacga
     1621 cggtcgtgtt cgtcgcctgc cgcagggact ctgcacacct ccgtttacgc atgtgcctgg
     1681 aggagttgga aatcgtcgtg ttcgggaaac attaaacaca ggatggcagc gatctgagcc
     1741 agcacatgat cagctagctc accatccgga tcgacggccc actgcatcgt cgcgccagcg
     1801 atgaccgagt gcaggagcaa ctcagctgcc gcaggagcac ctgggggcag tcgcttgcgg
     1861 atcccctcca ccaccgcgcg gttccgctgg atcgcaagcg tgcgtagctc cggcacctgg
     1921 agctcgtacc aggagatgag atagttcacc gagaagtcgt tgcgagtgtt catgctccga
     1981 acgagcacct gcaaaaattc ccagagccct tgcggccctg cgcctatcgg tatcgcattc
     2041 aggtaatgcc gcacctgctc gacgccgcgc tccatcatcc tcaccagcag cgtatcgcgg
     2101 ttggtgaagc gctggattaa cgctgcgcgg gagagcccca cctcctttgc tactccgctg
     2161 agcgtgaact ctatgggacc gcaacgcttc agcactacgg tggcggcctc gagtacctcg
     2221 tcatcggact tgagcttggg gcggggcatc agtgttcacc ttctgtatgg gttggggcgg
     2281 aggctgtggc tgccgccgcc attgtagcaa attgaagacg gagcgagagt agagccacga
     2341 gccccgcaaa cacggccgat acaacgaggc cagggagcgg accagcaagg tcgacaaacg
     2401 cgccggccgc aagcataacc atgggcgagg ctgacagcat caccgccgag accgtgccga
     2461 gtacccggcc gagaagttct ggcggcgtgc ggttgtagat ggcagcgttg agaatgggag
     2521 agactgagcc ggtcagcagt cccacgagcg cgcccaacaa catcagcacc ggcacgcctg
     2581 gcaactgtga aagcagaagc gagcccaccg cagagccaca aaatgccacc gccagccagt
     2641 tctgcgctga tatccgggcg ccgaccgacg catgaatggc aatgccaagg agaccaccag
     2701 cccccatcat tgaggagaac agcccgagct ctgctacttg gcgtcctgca tctacaaaca
     2761 gcgcaggcat gatgacgctg ccgttggcgc caacgatgcc cacgaagatc atcactatac
     2821 caaagagagg gcgcagcagg ggttcgctcc agagaaaagc gacgccggcg cgcatggaga
     2881 gagtcgccgt cgtggtcatc gtccgagcgg cacgcgcggg aagcacccac gcgccgagca
     2941 gacctgcaag gacggagcag aacgccgtca gcccgagcgt tggcgcagcg ccaagcaggc
     3001 cgattgcggc ccccccaagg gccgggccac ctagaatcgc gacgttcccg atcaccgctt
     3061 tcagtgacga gacgcgctca acggagagcc cggcgacgtg gccgagtttg ggcagctcac
     3121 tgtcctgcgc ggccataccg ggtgcgtcga acgcggcacc gagcaccacg caagcgatca
     3181 gcccagtgtt cgagagggcg ccaacggcat cgagcagtgg gatgctcgcc atggccacgc
     3241 cgcccaccac acccgagatc aatgcgacgg gcgcgcgccc gaaccgatcg acgaggccac
     3301 caccaaccca cgcgccgatg atggtcgcga tgacgctgct agcggccgtg gcgcccgccc
     3361 aggccgcgct ctttgtatga gacaggacga accatggaag cgcgagggcc gccaccgcgt
     3421 tgccgatccg gaagagaaag gtcgccgcga acagcgtcgc gagcgggcta tatcgacgtt
     3481 cgctcattcc gctgcggcga gctgcgcctt cgccgcagcg aggtactctt cgttacccga
     3541 gtcgagggcg aagagtgcgt aggtgaccgc cccgaacgca aggcgctccg cgatgtggtg
     3601 ggcgagccgc ggccacaccc ggccaccggc cgcttcatac gtgaggagga gcttcgcgag
     3661 cccctcttca ccaaagacca taaggtgcgc ggccatgtcg atggcagggt catcaacgcg
     3721 ggcctcgctc cagtcgatca tcccgctgac gcgctccgtg ttgtcgatga gcacatggcc
     3781 cacgtagaga tcgccatgca ccaccacgga gaaatctggc cacgacgaat cgtcgtcgag
     3841 ccagcgctgc caccggtgga ggcgcttgtc gttcaccacg aactcgcgtc ggacgcggtc
     3901 aacgtcgtcg gccaccttct gacgggcctg cgtcggtgta cggatgagca tccccgcatc
     3961 cacggcggcg gaaatgggga cggcatgcag ggcggcgagc gcggtcgcga agctctccgc
     4021 gaagacctcc gagtcctgcg gcacgaccca gtcgggcgtg gacgaaccag gctggatgac
     4081 catcgcagtc gagtcttcga gcatgggata ggcaacgagc tcggcgttgg ccacgcgcca
     4141 gtccggcacc gcgaacggca ggcgattctt gagcattgcc agcacccgcg cctctggttc
     4201 gaccttcgcg cttacctcgg ctcggcgcgg gatgcgcagc acccaccgac gtccatcgtc
     4261 gacggtggcg atcacgatcc tatagtcgag cccaagctca ttgacagtca gcgggccatg
     4321 gagcttgagc ccatgtcggg ctgcaagtgc gtacagttgg gaggtatcgg cggtcgtgac
     4381 tacggtcatg attcactcct gagggcttga cgggtttagc cacctaaatg taacagtcac
     4441 gtcggttata ttcaatccgg cactgttgca aagttagcga tgaggcagcc ttttgtctta
     4501 ttcaaaggcc ttacatttca aaaactctgc ttaccaggcg catttcgccc aggggatcac
     4561 cataataaaa tgctgaggcc tggcctttgc gtagtgcacg catcacctca atacctttga
     4621 tggtggcgta agccgtcttc atggatttaa atcccagcgt ggcgccgatt atccgtttca
     4681 gtttgccatg atcgcattca atcacgttgt tccggtactt aatctgtcgg tgttcaacgt
     4741 cagacgggca ccggccttcg cgtttgagca gagcaagcgc gcgaccatag gcgggcgctt
     4801 tatccgtgtt gatgaatcgc gggatctgcc acttcttcac gttgttgagg attttaccca
     4861 gaaaccggta tgcagctttg ctgttacgac gggaggagag ataaaaatcg acagtgcggc
     4921 cccggctgtc gacggcccgg tacagatacg cccagcggcc attgaccttc acgtaggttt
     4981 catccatgtg ccacgggcaa agatcggaag ggttacgcca gtaccagcgc agccgttttt
     5041 ccatttcagg cgcataacgc tgaacccagc ggtaaatcgt ggagtgatcg acattcactc
     5101 cgcgttcagc cagcatctcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc
     5161 gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca
     5221 gctccatcag caaaagggga tgataagttt atcacca
//
LOCUS       DADWTH010000187         5168 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.192, whole genome shotgun sequence.
ACCESSION   DADWTH010000187 DADWTH010000000
VERSION     DADWTH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.192"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1310..1531
                     /locus_tag="JNY02_003953"
     CDS             1310..1531
                     /locus_tag="JNY02_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435276.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPDLTD
                     EHVPLYIRRLEYYRRLYRPKQTESYWKKR"
     gene            complement(1585..2118)
                     /locus_tag="JNY02_003954"
     CDS             complement(1585..2118)
                     /locus_tag="JNY02_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435277.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="JNY02_003955"
     CDS             complement(2121..3047)
                     /locus_tag="JNY02_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBB9435278.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPDRGGAKKDSG
                     SLTPTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            3224..3622
                     /locus_tag="JNY02_003956"
     CDS             3224..3622
                     /locus_tag="JNY02_003956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435279.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELYYKKGMILQKLKSIKYDLDTYPNPDA"
     gene            complement(3817..4743)
                     /locus_tag="JNY02_003957"
     CDS             complement(3817..4743)
                     /locus_tag="JNY02_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBB9435280.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1457 a         1343 c         1088 g         1280 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact aaaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag atcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgacggcttt atcgaccaaa
     1501 gcagacagaa tcatattgga aaaaacggtg aacggttcgc gaaatccttt cgcgctccgt
     1561 gaggcgttgg acaaacgaag cgcgtcagtt tttccggctc ttacccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg tgagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtt cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg tgtatccagc
     2281 cattccctga tgtcatgccc gaccattcgc gcctgttcaa gatggcggta atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgggag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccggtcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cggtcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgaact
     3541 atactataaa aaaggcatga tattacagaa acttaaatcg ataaaatacg atctggacac
     3601 atacccaaac ccagacgcgt aagtattccc gttcacattc tggttccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccag cgcaatcatg
     3721 cggaaagaaa cgccattgct gggataaacg acgaaaaacc tacgactcaa aaggacgcaa
     3781 aaaaggcgct gttatctgat ataggtttta tttggtctac tctacaagac ctcgtttttt
     3841 cagttggtaa taccaggcgc ggctaattcc aagagtctcc cacggtttca acgttcgcgc
     3901 agatgtggca acagcaccac gcttagactt agcacctcca attttcccgc ccttgcgacc
     3961 acgtgcagcc tgaatttctg gcgtgtgcgt atcagccaca tactgtgcga aagtttccgg
     4021 cgtgaagttc ctgtgcgtgt atttcgcaat gctcttgcca atagcccgac attcagccag
     4081 tgaaagcgga acgggaagcg atgcattgta catttcgaca cgctggatca ccgcttcaag
     4141 ccactgtgag aacgctggcc agccctgacg aatcgccctg taagcccatt tgcgcgtcat
     4201 ttcgaacagg tggcagtttc gccccatccc gtaatgttta tcgatgctac gacgctctga
     4261 agcgctcaaa tcgagataat cagccagttc atcgagggta taggcttcct cgcgccactc
     4321 catcaccagc cattccaggt ggaacggatt tttgcaaatc aggccgctgt agttcacatc
     4381 cgcacaaagt ttctcacaca gcgaacgttc aaccgctgcg gcatatttca gcgccttaac
     4441 cgaggcatca ggcgcggttc tcacggcgat gttgagtgcg tagagcaggt gagcgtgtcc
     4501 gttcacagga tttttaacgg tgatgttcgg cgcgggagcg ttccggtcgc tccagtcaat
     4561 cgcagccccc tcgcggtcaa cgtcaaaaac cagccagaac tgtgcatgag gctggttctg
     4621 ctggatatac cgtgcaagaa gcgcgcgccc tttgccggag atacgaaggc cgaaagcgag
     4681 atcatccgtg tgatagggac ggtggggtaa attttcttcg aagtattgaa gcgcggcgct
     4741 catgtgttac gattccttcc aggttatcga ccgacatcgc ccctgtattt tcgtgaaagt
     4801 cttggcggat agacacgaat ttcaggggcg tttgtttttc agtcgcttac actaccccca
     4861 acgacaaaac ctgtcgagcc ttcccgcaaa tccgatccat ttaccataac tcccgttgta
     4921 cgtacccctt aaaacgccct caatcgcttt ctggcgcaaa aaacgcaaaa agacaagaaa
     4981 acaccaggcc aaaccagaaa aacgcgtcag aatcgttttc agggcgttgc ataactatgc
     5041 acgaaagcga tgtgcgatcg gtttcgtgcg ttctgaacac cgaacggctc aaaacgacaa
     5101 gttttctccc ctggtgcgtg gcgctctgac ggggtttcct gtttccaaac ttcacatctc
     5161 gccaggac
//
LOCUS       DADWTH010000188         5151 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.193, whole genome shotgun sequence.
ACCESSION   DADWTH010000188 DADWTH010000000
VERSION     DADWTH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.193"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            190..447
                     /locus_tag="JNY02_003958"
                     /pseudo
     CDS             190..447
                     /locus_tag="JNY02_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNY02_003959"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNY02_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBB9435281.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="JNY02_003960"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="JNY02_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBB9435282.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNY02_003961"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNY02_003961"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBB9435283.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNLISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JNY02_003962"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JNY02_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="JNY02_003963"
     CDS             complement(3605..4420)
                     /locus_tag="JNY02_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9435284.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            4517..>5151
                     /locus_tag="JNY02_003964"
     CDS             4517..>5151
                     /locus_tag="JNY02_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBB9435285.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1174 c         1225 g         1377 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgagat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtggcagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tctggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg acaggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagaagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctagcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DADWTH010000189         5144 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.194, whole genome shotgun sequence.
ACCESSION   DADWTH010000189 DADWTH010000000
VERSION     DADWTH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.194"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..253
                     /locus_tag="JNY02_003965"
                     /pseudo
     CDS             1..253
                     /locus_tag="JNY02_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="JNY02_003966"
     CDS             746..1492
                     /locus_tag="JNY02_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB9435286.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="JNY02_003967"
     CDS             1507..1929
                     /locus_tag="JNY02_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBB9435287.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="JNY02_003968"
     CDS             1987..2343
                     /locus_tag="JNY02_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435288.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="JNY02_003969"
     CDS             2436..2654
                     /locus_tag="JNY02_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBB9435289.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="JNY02_003970"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="JNY02_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3254
                     /locus_tag="JNY02_003971"
                     /pseudo
     CDS             3018..3254
                     /locus_tag="JNY02_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3447..3683
                     /locus_tag="JNY02_003972"
                     /pseudo
     CDS             3447..3683
                     /locus_tag="JNY02_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3683..4048
                     /locus_tag="JNY02_003973"
     CDS             3683..4048
                     /locus_tag="JNY02_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBB9435290.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4503..4658
                     /gene="hokD"
                     /locus_tag="JNY02_003974"
     CDS             4503..4658
                     /gene="hokD"
                     /locus_tag="JNY02_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBB9435291.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT         1407 a         1087 c         1421 g         1229 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
     4501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     4561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     4621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg gggagaaatc
     4681 cctcgccacc tctcatgtgt caggcatcct caacgcaccc gcactaaacc cgcttcggcg
     4741 ggttttttgt tgcgtgctga atacgcaggg tgaaaaataa ccatatattt gattatatac
     4801 acaacaaaaa ataaaagtca ttgtacttgc acattaaaca atcaaatata cggcgtgaaa
     4861 taaatatttt tcagattaat atttttgtct ctatgtggat ataaccgttt gtacttataa
     4921 acctggaggc atcgtggaaa aaataaagaa actatttagt agcaaatacg cagtcatacg
     4981 tcgtgatgac ctgtcagtta tagtcgaaat ggattacttc cctgaaaccc caaaatcaat
     5041 aatgtatcgt aatggtcgaa aggcaatttt tttaccgatg agggtaagtg acattatggg
     5101 aaatgataaa ctgctggatg aattgcgagt cagagcatcc tgtt
//
LOCUS       DADWTH010000190         5126 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.195, whole genome shotgun sequence.
ACCESSION   DADWTH010000190 DADWTH010000000
VERSION     DADWTH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.195"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..236
                     /locus_tag="JNY02_003975"
     CDS             <1..236
                     /locus_tag="JNY02_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435292.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="JNY02_003976"
                     /pseudo
     CDS             276..816
                     /locus_tag="JNY02_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /gene="dcm"
                     /locus_tag="JNY02_003977"
     CDS             883..2301
                     /gene="dcm"
                     /locus_tag="JNY02_003977"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBB9435293.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHVVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /gene="vsr"
                     /locus_tag="JNY02_003978"
     CDS             2282..2752
                     /gene="vsr"
                     /locus_tag="JNY02_003978"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBB9435294.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JNY02_003979"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JNY02_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBB9435295.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGEIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="JNY02_003980"
     CDS             3834..4751
                     /locus_tag="JNY02_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBB9435296.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAAWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="JNY02_003981"
     CDS             4830..5012
                     /locus_tag="JNY02_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBB9435297.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1225 c         1464 g         1185 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtaag cctggcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg tggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatctcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagagcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gcgagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg ggcgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcggc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DADWTH010000191         5116 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.196, whole genome shotgun sequence.
ACCESSION   DADWTH010000191 DADWTH010000000
VERSION     DADWTH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.196"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..366)
                     /locus_tag="JNY02_003982"
     CDS             complement(<1..366)
                     /locus_tag="JNY02_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBB9435298.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            892..1866
                     /gene="hemB"
                     /locus_tag="JNY02_003983"
     CDS             892..1866
                     /gene="hemB"
                     /locus_tag="JNY02_003983"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBB9435299.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="JNY02_003984"
                     /pseudo
     CDS             complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="JNY02_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="JNY02_003985"
     CDS             complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="JNY02_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBB9435300.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLLLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="JNY02_003986"
     CDS             complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="JNY02_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBB9435301.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="JNY02_003987"
     CDS             complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="JNY02_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB9435302.1"
                     /translation="VPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTH
                     YSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTD
                     SEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENIS
                     KLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAIIDTAQFLKEQGKVPAVAN
                     DYSQYVTSRFVQ"
BASE COUNT         1243 a         1408 c         1273 g         1192 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa
      541 ttgataaaag gacgatattt cagcatgttg cagataatgt tatttttgat gcatacgcgt
      601 tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat
      661 tattcaattt atagcagcac aaccaaacgc caaaaaaaat aataaccaag agctataaat
      721 aaagattaaa aatattttca gtgaatatga aattattgct atttctatta caaagatgtc
      781 ctggtctctc tttcattctc ttcttgtcaa catcgcgaca actttcgtaa aacactcctg
      841 ccccgcttca ggtatactgt gcaccttaat tccaaaaaca tcaggcagac catgacagac
      901 ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag
      961 acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac
     1021 tacaaagccg ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc
     1081 gaaattgaac gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac
     1141 cataccgatg aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg
     1201 cgcatctgca agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa
     1261 tacacttctc acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact
     1321 ctggaaaatt taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc
     1381 ccttccgccg cgatggacgg ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc
     1441 tttaaagata cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggcccg
     1501 ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca
     1561 atgaaccgtc gcgaggcgat tcgtgagtca ctgctggatg aagcccaggg cgcagactgc
     1621 ctgatggtta aacctgctgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact
     1681 gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg
     1741 ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt
     1801 gcgggtgcgg atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg
     1861 cgttaatttt tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc
     1921 cctcacccta accctctccc caagggggcg agggaaccgt ccacactcgt atcaccccgc
     1981 ccgataaaac ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag
     2041 gtaatcggca ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg
     2101 ttgccagcgc caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa
     2161 caaggcttcg ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac
     2221 aaacagcgcc tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg
     2281 atttttcgcg accgcctcgc gccagcgttg atgttcctcc tcggttttgc ggtatttgta
     2341 ttccgggaat gatttacgga aatcatgctc cgcacgcagc cagctcagca gctggcggaa
     2401 gggaacagag agcgcctcat aggccgcaat accgctggtt cagagcgtat caccgccggt
     2461 cgaaggtaac tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac
     2521 atcggtatgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat
     2581 ctcgtcaacc ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg
     2641 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaata ccacctgatg
     2701 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc
     2761 ggcacccgaa atttgtgcgc cgatatgcgg ccccagcggg gtaatgctca gacgttcact
     2821 cattgtactt ctccatgcca gggcgtcaga cggcgctgta acgcgcgcag acccagttct
     2881 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga
     2941 aattcacccg ctgactgaac cataaatcct aaaccgcgtg tcgcggcaat cagctccgcc
     3001 gctaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
     3061 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
     3121 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccctg ccagcgccga catcgccacc
     3181 ggcgcaaaaa tcgctaaata gatcagtaag atcttcgagg tttcaccaat accaaaccag
     3241 atcaccatca gcagcaaata agccagcggt ggcaccggac gataaagttc gattaccgga
     3301 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
     3361 accaccgccg cgagtagcgc ctgcacaatg cgcgtcaggc tggctgccag atgctgccac
     3421 agcgtggcgt ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt
     3481 tgcggcggcg gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca
     3541 gtgagtaaaa ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc
     3601 catttcagcc gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
     3661 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
     3721 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
     3781 ccagcacacg gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt
     3841 cttctatatc gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca
     3901 gcagagtttg catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcatcgagta
     3961 ataacagctg gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccat
     4021 cggaaagctg ccagatatag cgtttttctg cgccttccag ccccactttt ttcagcatct
     4081 ggtgcgcgat ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca
     4141 cgttgtcctg tacattgcgc cacggtagca gcccttcatt ctgaaaaact acgccacgct
     4201 ctgctcccgg tccctcaata cgcttacccg ccagttgaat gctgccatgc tgataaggca
     4261 caaaaccggc aatcagattc agcagggtgg ttttaccgca gccggacggc cccagcacca
     4321 ccagtagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
     4381 aatcggcata gagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg
     4441 aggtaacgta ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa
     4501 actgcgcggt gtcgatgatt gctttgttca ccggtccggt cagttctgcc gtttgttgct
     4561 gcggcgtcag ataggtattt cccttcacca gccccggaac gtcaccttca ggcacgccgc
     4621 ttaaacgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtca gggttagcaa
     4681 tgtacggttg ctgtgcatcg atggcgcttt tggcgaacgc tttcacgacc tcaggatgtt
     4741 tctcggcaaa atctttgcgc accacccaga cgtccagcgt tggcgcaccc cactgcccga
     4801 cctgttcaga atcggtcagc accttgccgt ctttttccag ggcgttaacc gccggtgccc
     4861 agacataagc accatcaata tcaccccgct gccatgcggc gataatcgcg ggcggctgca
     4921 ggttcacaat ctccactagc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc
     4981 tgtagtgggt ggtggagata aacggtacgg caatgcgttt accaatcaaa tcttcgggtt
     5041 tgctgatagt tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga
     5101 agacttcaat cggcac
//
LOCUS       DADWTH010000192         4992 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.197, whole genome shotgun sequence.
ACCESSION   DADWTH010000192 DADWTH010000000
VERSION     DADWTH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.197"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="JNY02_003988"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="JNY02_003988"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBB9435303.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNY02_003989"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNY02_003989"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBB9435304.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="JNY02_003990"
     CDS             2152..2610
                     /locus_tag="JNY02_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBB9435305.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="JNY02_003991"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="JNY02_003991"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBB9435306.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="JNY02_003992"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="JNY02_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBB9435307.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="JNY02_003993"
     CDS             3816..4493
                     /locus_tag="JNY02_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBB9435308.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="JNY02_003994"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="JNY02_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBB9435309.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1213 a         1186 c         1315 g         1278 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac cctaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga atgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatc agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctgggga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg atttcaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tc
//
LOCUS       DADWTH010000193         4951 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.198, whole genome shotgun sequence.
ACCESSION   DADWTH010000193 DADWTH010000000
VERSION     DADWTH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.198"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..611
                     /locus_tag="JNY02_003995"
                     /pseudo
     CDS             1..611
                     /locus_tag="JNY02_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
     gene            632..1292
                     /gene="csiR"
                     /locus_tag="JNY02_003996"
                     /pseudo
     CDS             632..1292
                     /gene="csiR"
                     /locus_tag="JNY02_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="JNY02_003997"
     CDS             complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="JNY02_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBB9435310.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1826..1984)
                     /locus_tag="JNY02_003998"
     CDS             complement(1826..1984)
                     /locus_tag="JNY02_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBB9435311.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2167..2466
                     /locus_tag="JNY02_003999"
     CDS             2167..2466
                     /locus_tag="JNY02_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9435312.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2476..3000
                     /gene="ygaP"
                     /locus_tag="JNY02_004000"
     CDS             2476..3000
                     /gene="ygaP"
                     /locus_tag="JNY02_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBB9435313.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="JNY02_004001"
     CDS             complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="JNY02_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBB9435314.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4119..4556
                     /gene="alaE"
                     /locus_tag="JNY02_004002"
     CDS             4119..4556
                     /gene="alaE"
                     /locus_tag="JNY02_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBB9435315.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4593..4943)
                     /locus_tag="JNY02_004003"
     CDS             complement(4593..4943)
                     /locus_tag="JNY02_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBB9435316.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
BASE COUNT         1260 a         1168 c         1165 g         1358 t
ORIGIN      
        1 tgccggggct gaaagccgtt ggatcttatc gctcggttct ggaattgctc aatattcccc
       61 atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagttgc ctgaactcgg
      121 ccctgtatac cgcctcaagg atgctctact ccttaagccg tcgcggtgat gctcccgcgg
      181 taatgggtaa aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc tccaccggcg
      241 cagcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt aaatttctga
      301 tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc
      361 ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg tggctttatc
      421 cctggctcac ctagctggtc atcggcttta ttacctttgt gttggtagtg atgctattcc
      481 gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg attatctgta
      541 ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata
      601 atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg atggctatcg
      661 ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag
      721 tttactgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagtac tttctcaact
      781 ggtggtggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc
      841 agagcaggag ctgctctgac atagacgccc gcgccaatat ggaagcgatg ctggtcagtc
      901 tggcgattgc ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc
      961 tcaataagct ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc
     1021 atcaggcgtt tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc
     1081 gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc
     1141 tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac
     1201 tggcgcgaga tacagcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc
     1261 ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc
     1321 cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg
     1381 tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta
     1441 acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg
     1501 gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg
     1561 ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca
     1621 ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc
     1681 tgacctgtaa ccgcatccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc
     1741 atagctacct cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt
     1801 taacgccgcg ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca
     1861 aaccggggat atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc
     1921 ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac
     1981 ccatatgtac tccctataag aaaattactc attgtttaaa aagagacttt atctcttaag
     2041 cgtagtaatt ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg
     2101 ctgaggctgg tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa
     2161 ataaatatga ctgaactcgc gcaattacag gccagtaccg aacaggcagc ggccttattg
     2221 aaagcaatga gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc
     2281 accagcgcgg gagagctgac gcgcattacc ggactgagcg cctctgcgac atcacagcat
     2341 ctcgctcgta tgcgggacga agggcttatc aacagccaac gggatgccca acgcattcta
     2401 tattccatta aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt
     2461 ccgtaaggag tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc
     2521 gcacgcggcg caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt
     2581 cctgaagcag atctggctcc attatccgtg ctggaacagt caggtcttcc acctaaatta
     2641 cgtcgcgagc aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat
     2701 aaattagcag cgattgccgc ccccgcagaa atatttttac tcgaagatgg gattgatggc
     2761 tggaaaagag ctggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc
     2821 caggtgcaga tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta
     2881 aatagcggtt tcttcttatt aagtggcttt gttggtgccg ggttactgtt tgcaggaatc
     2941 agcggttttt gtggaatggc aaggttgtta gataagatac cgtggaacca acgagcttga
     3001 gaagcgacgc cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat
     3061 cgtcgagaga tttaccttct gccagcgcct gagcaattgg cttcggtgta cggccctgac
     3121 cggtccaggt tttagtttca ccgttaacat cagtgaattt atatttcgcc ggacgcggct
     3181 ggcgtttttt accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt
     3241 taattccgtc agctttcatc agctccagcc aggtgctaat tttttcctga cgctcagcca
     3301 gttcacgctg ctgctgttct tcttcttcac gcctttcttt agtgacaacc ctgaattttt
     3361 cgagcatttc ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa
     3421 tgttatttaa actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca
     3481 aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc
     3541 tatttacagt cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt
     3601 tcaagattat atattagcca ttttgatgag taatttaggt tattggtgaa cagagtggcg
     3661 ctctctgctc gctaactccg taagcctgac aaaaccgaag tgaataaaaa tgcaatataa
     3721 cagattaaaa gaaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca
     3781 tcaaaaatgc tgtaaatgac ccggtcacga caaacaccga attctctacg cttatagcgt
     3841 tacctccccc ccaaactttc attagtgaaa ttaaaggcaa ggccatatcc aattgattta
     3901 attgaaaata aatcatatcg acatgtaaaa atccacaatg tacaaaaaac gagctgttac
     3961 ggaatatttt atctacaaaa actgactaaa taaaaatttt tcactaattg attagtcata
     4021 gccagcgata tacgctatgc gaaaatacag atggcaatga gatccactgc tttcatctcc
     4081 attaacatcc cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct
     4141 tgcgtcatgc agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt
     4201 gtattgaagt tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag
     4261 cgattccggt gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc
     4321 gcgcggcacg caaaggctgg ataaaaaatc tggctgatat cctggcttat gtgacgttcc
     4381 agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg
     4441 cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc
     4501 tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgactct
     4561 tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc
     4621 aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcgtgt
     4681 cgctgtcgct cgcctgctaa atagagcgga aattgctggt aatgatcgca agttttaata
     4741 tcggaagcgg gttcagccag tgttgaactg cgttctttta atgttggatg aattaccaga
     4801 gcggtacgtc ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa
     4861 ccatacgctt tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc
     4921 acctcgtctg gtcgtaaata catatttgat c
//
LOCUS       DADWTH010000194         4868 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.199, whole genome shotgun sequence.
ACCESSION   DADWTH010000194 DADWTH010000000
VERSION     DADWTH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.199"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..213
                     /locus_tag="JNY02_004004"
     CDS             <1..213
                     /locus_tag="JNY02_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB9435317.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="JNY02_004005"
                     /pseudo
     CDS             356..577
                     /locus_tag="JNY02_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(598..660)
                     /locus_tag="JNY02_004006"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="JNY02_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="JNY02_004007"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="JNY02_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HBB9435318.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHGSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="JNY02_004008"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="JNY02_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="JNY02_004009"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="JNY02_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HBB9435319.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLNPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4665..4784
                     /locus_tag="JNY02_004010"
                     /pseudo
     CDS             4665..4784
                     /locus_tag="JNY02_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1668 a          894 c          921 g         1385 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg cgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatggctct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ctttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatgggtt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttaacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaaacc gtctggtctg ttacctccgg
     4621 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatggaa taccggacct
     4681 ggatcactga agctttacgc cttcatttcg aagaacattt accacgggtt gtggccggac
     4741 gtcgcctggg cgtaccaaaa tcaactgtct gtggtatgtt cgtgtgaacc gcccccggta
     4801 tcccgctcat gctggcgaac ccagacacgc agtgaaccgc cccgggtttc ctggagagtg
     4861 ttttatct
//
LOCUS       DADWTH010000195         4845 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.200, whole genome shotgun sequence.
ACCESSION   DADWTH010000195 DADWTH010000000
VERSION     DADWTH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.200"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            13..429
                     /locus_tag="JNY02_004011"
     CDS             13..429
                     /locus_tag="JNY02_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435320.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1081..1236
                     /gene="hokD"
                     /locus_tag="JNY02_004012"
     CDS             1081..1236
                     /gene="hokD"
                     /locus_tag="JNY02_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBB9435321.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1278..1463)
                     /locus_tag="JNY02_004013"
     CDS             complement(1278..1463)
                     /locus_tag="JNY02_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435322.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            1404..1682
                     /locus_tag="JNY02_004014"
     CDS             1404..1682
                     /locus_tag="JNY02_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435323.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            1684..2742
                     /locus_tag="JNY02_004015"
     CDS             1684..2742
                     /locus_tag="JNY02_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBB9435324.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHMKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTSTSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2743..3108
                     /locus_tag="JNY02_004016"
     CDS             2743..3108
                     /locus_tag="JNY02_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBB9435325.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            3105..3815
                     /locus_tag="JNY02_004017"
     CDS             3105..3815
                     /locus_tag="JNY02_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBB9435326.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQVGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFV
                     MRTRSKKRRHFQSKVLQK"
     gene            4014..4089
                     /locus_tag="JNY02_004018"
     tRNA            4014..4089
                     /locus_tag="JNY02_004018"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4047..4049,aa:Ile,seq:cat)
     gene            4091..4167
                     /locus_tag="JNY02_004019"
     tRNA            4091..4167
                     /locus_tag="JNY02_004019"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4125..4127,aa:Lys,seq:ttt)
     gene            4170..4245
                     /locus_tag="JNY02_004020"
     tRNA            4170..4245
                     /locus_tag="JNY02_004020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4203..4205,aa:Thr,seq:tgt)
     gene            4249..4323
                     /locus_tag="JNY02_004021"
     tRNA            4249..4323
                     /locus_tag="JNY02_004021"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4282..4284,aa:Gly,seq:tcc)
     gene            4616..4831
                     /locus_tag="JNY02_004022"
     CDS             4616..4831
                     /locus_tag="JNY02_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBB9435327.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1286 a         1075 c         1283 g         1201 t
ORIGIN      
        1 gcggtaatga aaatgactga attaacaaaa gaacaattaa tcgaagaagc caaattaaaa
       61 atagcgatta cgaaatgcca ccccaattca gggatggcgc gagtagaggg cgagttattc
      121 aaaattgcac gggcatcgct ggaagcagag ccgatagcgt ggcgatatcg ctacgtgaaa
      181 aaaggtgtta tggactctca gggggagttg tgggttggtg actggaaata tgtaccgaaa
      241 aaagaggatt gtaacgacag gccgaactat gaaattcagg ccttattcac tgccccacca
      301 gtcccggtta catcagaaga actggttaaa gctgtgcact tttatgaaca actaaaacgc
      361 gaaaatccac cagcatccgg caaccagatt aatgggttaa ctatgtcggt taaacgacca
      421 gccaactgaa aaagcggaaa cctgattata ggttgccaga taaggcaata agctacctgg
      481 cgcggaacgg actgataagt atggggaatg ttttacgatg agtatttaga ctaaagagtt
      541 tgtaacgcta tgtaagtgat tttttctggt ttagatattt atatgtccgg ccaaattgag
      601 gtgtgtttaa atgttattgc acattgattg tagggggaat aatgaaaaac gcattgcagt
      661 ttttgtttgt tgcgttctgg ttgttcgcat catgtatgcc catcatcttc acagcaaggt
      721 atatggaaaa aattgatgtt ttgatattaa tgtttggaca tataaatgcc ctttttttag
      781 gggtgttcat ggcggtcatg tgcattgaat actggctgta aatacagcga acgctattgg
      841 tttagttgga tatttactgt gccggacaaa aacggtttgc agggaaatct tagttaagta
      901 gaatgactgc gggtgcttga ggctatctgt ctcaggcatg aacaccaaaa ggcagataga
      961 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa
     1021 tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
     1081 atgaagcagc aaaaggcgat gttaatcgcc cttatcgtca tctgtttaac cgtcatagtg
     1141 acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg ccagacggag
     1201 gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgggg gagaaatccc
     1261 tcgccaccgc tgatgtgtca ggcatcctca acgcacccgc acttaacccg cttcggcggg
     1321 ttttgttttt tctggtcgtt ctggtttaca atccatccgt cagcctgaac aactggcacc
     1381 tgctgcgcca gcagagaaaa cagatggcgc acgataccaa attttacaat tcggataact
     1441 ctgccgcccc tgccagcagg cacgggcggc gttctcatgc attcaaatct gactggtatc
     1501 agcacgaccc ctgcaccgaa gaacaggctg aatggctgat tcagcgctac cgcagacacg
     1561 gatacgagat taagaaagcc ctcagtctcg attatcgtca ctggataatc ttcgtcaggc
     1621 tcccttattc cgaacggcca ccgcgtccgt cctgcacatt ccagcaacgg atctggaggt
     1681 aatgtgcggg tattacttcg acctgttctg gttccggaac tcgggctggt ggtccttaag
     1741 ccaggccgtg aatccatgcc ggtattccac aatacccggg tactggtgga gccggaaccg
     1801 aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg ttcgccagcc gctggtggaa
     1861 gacaaaacat tgctgccgtt tttcagtaac gcacgggtga ttcgtgctgc tggtggtgct
     1921 ggcgcattgt ctgactggct gttgcgccat atgaaatcct gccagtggcc acacggcgat
     1981 tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg gcgcaatggt gttgtgctgg
     2041 cactgcgaca accagttgcg tgaccagaca tccgaatcac tcgagcaact tgctcatcaa
     2101 aacctgtcag catggatgat tgacgtcatc ggtcacgcaa taagcggtac gcaggagcgt
     2161 gaattatctc tggctgaatt atcctggtgg gcggtccgca atcaggtggc ggacgcgcta
     2221 ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg aaaaaatccg ctcaatgtac
     2281 cgtgaaagcg acatcgtgcc gggagagcag acctccacca gcatactgaa gcagcgcaca
     2341 aaaaatcttg cgccgctgcc tcacgcccac cagcaacaga atccaccaca ggaagagacg
     2401 gtggtcagca ttgccgttga tccggaatct ccggctcagt atctccagcg ccagaaacca
     2461 caacgggaag agatgcctgt atacacgcgc tgggtaaaaa cgcagaaatg catgacgtgt
     2521 ggcaatcagg cagatgatcc gcaccacatc attggtcatg gactgggagg gatgggaaca
     2581 aaggctgacg atttgtttgt tattccgctg tgtcgtaaat gccataacga actacacgcc
     2641 ggggtaaaag attttgaaga aaaacacggt agtcagttgt tgttgctgat tcgtttttta
     2701 atgcacgcga gaaattcggg tgttctgaag tggaaagcat aaatgaccga acgcatagaa
     2761 tttgttttgc cttacccgcc aacggtgaac acctactggc gacgtcgtgg cagcacatat
     2821 tttgtatcaa aagccggtga gcgttatcgc cgtgatgtgg cgctaattgt tcgccagcag
     2881 cgactgaaat taaacctgtc cggaaggctg gcgataaaga ttattgcaga gccaccggat
     2941 aagcgccgtc gtgacctgga caatatcctg aaggcaccgc tggatgcgct gacgcatgct
     3001 ggactactta tagacgacga gcagtttgat gaaatcaata ttgtgcgcgg tcagctcgtt
     3061 cctggtgggc ggctggggat aaaaatcaca gaactggggt gcgcatgaat aaccagtatt
     3121 tacagtttgt gcgtgagcag ctcattatcg ccaccgctga tttgagtggg gcaacaaaag
     3181 gtcagcttga agcctggcaa gagaatgcca tgttcgatac agggcgttac aggcgtaaaa
     3241 aaatccggta ccgcgatgaa gtgactggaa aaatgataac gcgggataat ccaccaatcc
     3301 cgggaaagca atcgctggcg aaggggacgt caattcctct ggtcagtccg gttgagtttt
     3361 cgacatcatc gtggcggcgg gctgttctgt ctcttgaaga acatcataaa gcctggttgt
     3421 tgtggtgtta cagcgggagt atttgttggg aatatcagat cgcgataaca cagtgggcgt
     3481 ggaatgaatt taatgctcaa tccggtacca gaaaaattgc agggaaaacg caggaacgcc
     3541 tgaaaaaatt aatctggctg gcggcgcagg cagtaaaagc agaacttttt ggtggggaag
     3601 gttatgaata ccaggttggt ggggaaggtt atgaatatca ggagctggca ttactggcgg
     3661 gagtaacaac caaaaactgg tccaaaacat ttactggtca ctgggttgta atgaaacaca
     3721 tttttcaccg actggatagt gaggctttat tgtttgtaat gagaacacgt tcaaaaaaaa
     3781 gacggcattt tcaaagcaaa gtgttgcaaa agtagattga aaggcatata ttcatgcaaa
     3841 tctgatattt tgccgatttt gtacgtgatg gcaaaagcaa acaaaacccg cccacaagcg
     3901 ggttttttgt gccacttatc tcggatagac atggtgaatg cgctggtgga ggagataagg
     3961 gtgatttttg aatgcttgca acattgattt cgtaacgtta ttatcctgcg ctcggccctt
     4021 tagctcagtg gtgagagcga gcgactcata atcgccaggt cgctggttca aatccagcaa
     4081 gggccaccaa ccgccactag ctcatcagga aagagcgtca accctttaag ttgagtgtgc
     4141 gaggttcgag tccccggtgg cggtccagtg ccgacttggc tcagtaggta gagcaactga
     4201 cttgtaatca gtaggtcacc agttcgattc cggtagtcgg caccatatgc gggcatcgta
     4261 taatggctat tacctcagcc ttccaagctg atgatgcggg ttcgattccc gctgcccgct
     4321 ccagttagag tctttcagtc tgcgatgatg ggaaatcccg gagtgactga aagacgttta
     4381 agttatgaat gatcgctttt ttttgcaaaa ttgctgtgca gaaatactaa ccttcgggca
     4441 ggcgatcatt cataagcact ctgcttttat tccgattaac tgtgagtggt ttgttggata
     4501 gagtgctttc cttactgtat atatcgtttc gcccgctttt gcggtttttt cttttcaaat
     4561 ccctttcatt tctcagtgta aaactacgcc atccgttatt tgcggaggtg aggctatgaa
     4621 atccatggac aaaatttcaa cgggcattgc ctacggcacc tccgcaggca gtgctggcta
     4681 ctggttttta cagtggcttg atcaggtcag tccgtcacag tgggctgcga ttggtgtact
     4741 ggggagtctg gttctgggct tcctgactta tctgacaaat ctgtacttca aaatcagaga
     4801 agacaagcgt aaggctgcga gaggtgaata atgtcgccat catta
//
LOCUS       DADWTH010000196         4820 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.201, whole genome shotgun sequence.
ACCESSION   DADWTH010000196 DADWTH010000000
VERSION     DADWTH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.201"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1103)
                     /locus_tag="JNY02_004023"
     CDS             complement(<1..1103)
                     /locus_tag="JNY02_004023"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBB9435328.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
     gene            complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JNY02_004024"
     CDS             complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JNY02_004024"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBB9435329.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3116..4765
                     /locus_tag="JNY02_004025"
     CDS             3116..4765
                     /locus_tag="JNY02_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBB9435330.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1221 a         1243 c         1158 g         1198 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac accaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aaatccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
     1201 aagcagaaaa acgacttttt ataatataaa catttaacat gataatatta aaaacagact
     1261 ttattatcgt aataaaaatc gcccaaacat tcctgttcgg gcgatcaaat taattattgt
     1321 ttatcctgca tatacggcgt gtacaccccg ttcagactgt gcaggaaagc tacgatatca
     1381 tccacatcct cctgcggcag ctctttgcct acctgatagc gcagcatcag tttcaccgcc
     1441 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
     1501 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtcacatt catacgacca
     1561 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagcaccaa cggttcaaag
     1621 gaacgtccgc cgagaataat accaccatga caagttgcac atttattatc tttaaataat
     1681 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ctccacgcaa ccatttatca
     1741 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
     1801 tcgccactga aaccttgcgg ataaacttcg aggaactgcg ctttaagctg cggatctttt
     1861 tccagcttag caataatttc gtcccaggat ttcgacgcca tttcaatcgg gttcaacggc
     1921 ggtccacctg cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
     1981 aatactgagt taaataccgt cggcgcatta atcggcccaa ctgcgccacc aacaccaatc
     2041 gatgtttttc tgccatcgac gccccccgca ttcaacgtat ggcaatgagc gcatgaaatg
     2101 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
     2161 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
     2221 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
     2281 cgctcttcat cactcacctt acccgcccag tgtagcgcgg tataacgcgt tggtggcata
     2341 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     2401 gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     2461 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggc
     2521 gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta aaaccttatt attttcactg
     2581 acagcagatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     2641 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     2701 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     2761 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagggaga tacgcttttt
     2821 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     2881 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg
     2941 aaaaacaaca ccaaatccgt atttttataa attccattga tattagtgcg ttatattttt
     3001 tgacaagtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     3061 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     3121 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta
     3181 tgaactggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     3241 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     3301 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     3361 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     3421 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     3481 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     3541 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata
     3601 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     3661 gggactggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     3721 gatgatcgaa aactacggtc acatccccaa cggcaaccgc acctattatt tgagccgctc
     3781 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     3841 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     3901 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     3961 ccgttactgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     4021 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggagcggc
     4081 ctccggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     4141 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     4201 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     4261 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     4321 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     4381 ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tacgcagccg
     4441 gttactgaca cctggcggga ttttggcaag cgagtacgaa accggtgaac agtgggataa
     4501 acccaacggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     4561 tgaccttctg ggtgatgaaa tcgcgcgcag ctggctgaaa acggtgaatc agttctatct
     4621 ggaacagcac aaaatgatcg agaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     4681 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     4741 aattggtttg tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct
     4801 gtaaaattat cctcttcagg
//
LOCUS       DADWTH010000197         4735 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.202, whole genome shotgun sequence.
ACCESSION   DADWTH010000197 DADWTH010000000
VERSION     DADWTH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.202"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..181
                     /locus_tag="JNY02_004026"
     CDS             <1..181
                     /locus_tag="JNY02_004026"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBB9435331.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
     gene            524..1240
                     /locus_tag="JNY02_004027"
     CDS             524..1240
                     /locus_tag="JNY02_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB9435332.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1696..1771)
                     /locus_tag="JNY02_004028"
     tRNA            complement(1696..1771)
                     /locus_tag="JNY02_004028"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1736..1738),aa:Asn,seq:gtt)
     gene            complement(1872..3499)
                     /gene="yeeO"
                     /locus_tag="JNY02_004029"
                     /pseudo
     CDS             complement(1872..3499)
                     /gene="yeeO"
                     /locus_tag="JNY02_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3504..3579
                     /locus_tag="JNY02_004030"
     tRNA            3504..3579
                     /locus_tag="JNY02_004030"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Asn,seq:gtt)
     gene            complement(3617..4567)
                     /gene="cbl"
                     /locus_tag="JNY02_004031"
     CDS             complement(3617..4567)
                     /gene="cbl"
                     /locus_tag="JNY02_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBB9435333.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1438 a         1110 c          970 g         1217 t
ORIGIN      
        1 tgtttcagat aaaccgttac atggtgagat taagcttccc ggccaggcta accgttttta
       61 tgaaggcgct atttccgaac acctgcaaat tggcattcgg gcgatcgatt tgctgcgcgc
      121 agaaggcgac cgactgcatt cacgtaaatt acgaaccttt aatgagccgc cgttccgata
      181 ataagttatg aaccttctta tcagaaatag atgtgaagga ttaagtaaga catctggcaa
      241 atagcctgca ataaacgcgg gctgcttgct tccttgggca gatgcgagtt ttattatcgt
      301 cttaatgatt tccacatatt aaaagcaagt atgctttcaa aacacaatta taaaaaatcc
      361 cgccaacaac attagttttt ataaaattaa atataagatt atggctttag aatattttta
      421 tttctaatag acgagatttt tcctgttatg atataatatg ccgaattaac acatgttaac
      481 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
      541 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      601 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
      661 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      721 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
      781 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
      841 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      901 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
      961 tatttttgaa attttgcttg aagctgaagt tgatgttcgt gatgtaactg aagaagaagg
     1021 taatattgtt atttatactg aacctgctga ccttcataaa ggaatcgcgg cgctaaaagc
     1081 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
     1141 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
     1201 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatttta aagaaatctg
     1261 tctttatggc agatttcttt aatctcatat aattcttata aaaaatataa tgtttaactc
     1321 gtcatattgc ttataaaaaa tccccgttcc cagagaaata atatttatta aaatggcagg
     1381 tcttcttttt ctgattaccg aagacaaagt ggcaattacg tatagtttcc cgataaagac
     1441 gcgacagcta catcccgcat aaggcatttt tctctttatc tttgtacggt acttcatgga
     1501 acagaatttt tgaccttgtg aatcgtgatg tctgttgggg aggggcaatt tgctcactga
     1561 agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggc cactttttta
     1621 caaagtggcc gggtaaaaat aatttttatt ttttaactgt cctgagtctc atagagatgt
     1681 ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag
     1741 tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat
     1801 gcccacccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg
     1861 cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg ctcggatcga
     1921 gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca
     1981 gcccagtagg caaacattcc catccagaca ccaaccacac cccagccaag catgattccc
     2041 tgcacataac cgactacaac ccggcaaccc cacatgctca acatagaaac ccacatggcg
     2101 taacgggcat cacgagcacc cttaaatcca gcgggtagca cccatgaggc ggaccaaata
     2161 ggcataaata aagcatttag ctaaatcaga atcacaacga cgtgtttaac ctgtggatcc
     2221 tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt
     2281 aatccaagag tggacagcca gaacacatgc cgcaactgaa tctctgcttg cgctatctgc
     2341 cctaccccca accttcggcc tgtaatgatc gtagaagcag aaccgagcgc acttccgggt
     2401 aagttgataa gagccgcaat tgagaacgcg ataaaatttc cggcaataac gctggtcccc
     2461 atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca
     2521 ctcgctggaa taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa
     2581 tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt
     2641 gcaactgcgc caatataacg agaaatggtt aaacccaacc cagccccgac caggagaaaa
     2701 ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca
     2761 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg
     2821 cagcggctgg ataactgagt acggtcagct ccagataagt caacgccagt gctttaactt
     2881 ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaattagcg
     2941 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
     3001 ctcgtcgtcg atcccgctta ccgagactaa atgctacaac gacagtagta ccaagatcga
     3061 tagctgcaaa aaaagccata atgaccatat tgaagctgtc cgccaacccc acgccggcca
     3121 tcgcatcttt tcccagccag ctgaccagaa aagtgctcag gacccccatc aacaggacac
     3181 aggcgttctc catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt
     3241 tgtagctctt gcgtttagag tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
     3301 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat gatgagggag
     3361 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
     3421 atagaaacgg caaacagttg ggaaatttaa aaatcgggtt tgacaaaata tttttcgccg
     3481 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
     3541 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
     3601 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
     3661 ggcgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag cgccaaacat
     3721 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     3781 gccgcgtatc caggcggatt aaattctctt cctcttgttc gccgctggat tgttcggcaa
     3841 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgtgcac
     3901 ttaatacaat atctgccggc aaacctttgc gggtaaatgc gtcatcagtg cgtgagcgcc
     3961 ccgtaattcc ctgtcggtaa gtaattaacg gccacttcgc tattgattcc agcgtcaatg
     4021 gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc caacaaaacc
     4081 acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     4141 cttcgccatt ttgcaacaac gtcgcaattt cctgtggcga accctggatt agctcgagcc
     4201 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     4261 gagtatgcgt cgttgcaata gtgagaacac cagacgtatc gttggtaaac aggtctgcaa
     4321 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     4381 ccggttcagt catccccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt
     4441 catcctccag ttcccgaata tgacggctaa cgcctgactg tgaggtaaaa agcatattcg
     4501 caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga
     4561 aattcacggt aaactccggg cagttcagat atcccgttat tgttaaagtc taatgcccgg
     4621 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     4681 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actct
//
LOCUS       DADWTH010000198         4713 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.203, whole genome shotgun sequence.
ACCESSION   DADWTH010000198 DADWTH010000000
VERSION     DADWTH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.203"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(35..145)
                     /locus_tag="JNY02_004032"
                     /pseudo
     CDS             complement(35..145)
                     /locus_tag="JNY02_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(469..1146)
                     /gene="ospD1"
                     /locus_tag="JNY02_004033"
     CDS             complement(469..1146)
                     /gene="ospD1"
                     /locus_tag="JNY02_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HBB9435334.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNSHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(1517..1654)
                     /locus_tag="JNY02_004034"
                     /pseudo
     CDS             complement(1517..1654)
                     /locus_tag="JNY02_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1736..2034)
                     /locus_tag="JNY02_004035"
                     /pseudo
     CDS             complement(1736..2034)
                     /locus_tag="JNY02_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2623..2782)
                     /locus_tag="JNY02_004036"
                     /pseudo
     CDS             complement(2623..2782)
                     /locus_tag="JNY02_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2786..4015)
                     /locus_tag="JNY02_004037"
                     /pseudo
     CDS             complement(2786..4015)
                     /locus_tag="JNY02_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(4035..4382)
                     /gene="tnpB"
                     /locus_tag="JNY02_004038"
     CDS             complement(4035..4382)
                     /gene="tnpB"
                     /locus_tag="JNY02_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBB9435335.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(4382..>4713)
                     /locus_tag="JNY02_004039"
     CDS             complement(4382..>4713)
                     /locus_tag="JNY02_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435336.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
BASE COUNT         1168 a         1115 c         1033 g         1397 t
ORIGIN      
        1 agtatgatcc tacccacgta atatgaacac aggcttaagt gaggttctgg ttttcaaatt
       61 gttcaggact gagacctcca caccaactgt gccgtcgtca gcgattgtaa tcgcacttga
      121 tataattaaa cactgtatat ttcatttgtg gagtctccag tctgaggggg ctttatgcat
      181 tccgtattgg gcactatgat gccagaaatc tgcaaggctc cacaccttcc tgtatttccc
      241 ttcctctatc acagtgcact ttacttaata gggtgggatg tgttcattac atttattgtg
      301 ttttttatga ttgtttttgc gtccagtgtc gttcgtcatg ggaaactggt tctatgggta
      361 gtatcgctca gtctagttaa ctgtttgttt ctttttagaa attaagcaga tcacacaatt
      421 cggggagaga tccttctaag tgcaattctt tgcagctatt tagcacactt aaaacaactc
      481 ttgtattttg tcagaaataa tattaaacat gtcttgttca ttattgttaa gtctgtctgc
      541 tatttctgta agttttatcc cactatgatg gcatatattt atatatgcca tcatagcctc
      601 gatattccct ttccctgcgg ccagaaataa tcctggtaac ccctcattat ctcttacatc
      661 caatagttta atcgtttctt ttggtgataa atcaaaagtt ttaataaaat ctccataagc
      721 ttgaatcgca gcggaatgac cttcttgaag agctatgaac aaagcagatt ccccagcatt
      781 atttcgtgct gcaagaactt ctagtttttc ctctttgggt atttcggctg ccaacagcga
      841 ttcaccgtat ttacgcgctg catcggcatt tccatgccaa atcaatgaaa acaaattatg
      901 gctgttatac tccttattat tctttgtcgg aatggatatg ctctgtattt tggccaattt
      961 tctgaaagaa acaccactat atcccccccc tgatttttct tcattgatgg cgtgggatat
     1021 agcggtattt gtcacgttaa tgatattatt tttcattcct ttattttcat tagcagtatt
     1081 gctgcctatt attaggtgca tatttttgtt gtttgctgga tgtaatccat agttatttat
     1141 tgacattttt gttcatcctt tttgctaaaa aaaacaataa cctatttttg taatgtaata
     1201 gatattagtc tattgctaat ctaaaaaaac cattgccaca ttgaatggct atattcacca
     1261 ataaattctt tcgatttcgt atattgacat atcgtagata tatcttatcc ctaagattca
     1321 ataatcgagt ccgtttacca ttctgtgcaa gcgccttcac tcagaagtga ccgtcaaggc
     1381 gatcaccaaa cttgataata aagcagctca ttgccatacg ccagtcctta aggggcgttg
     1441 tccatttctg tgatgctgcc tagatttaac tgccagctcc ggcttatcta catctaaata
     1501 tgtgagccta ataccgctac actttgtcca gactatgttt ttctccggga agtgggctaa
     1561 ccgtctccat aaagtgacga acttttttca acagtggcca tattgaacgg cactgatgat
     1621 tacgggttat tgtctcatga agagccggcc acatcactga cagcagtgac ttcaggcctt
     1681 cagcaccaga cagcctgaac cagttaatcc atcgcccgac agatttagta atctagggct
     1741 ggtttttctt cagctgttgc ggttcgaaag tgccattacg atcacgcaga gtacggattt
     1801 ccagaggttc atctccagta gtgataatct tcgttaagca accattgcgg gaattgctgc
     1861 cagatctaag ctgatttttc tcgcacccaa gatgatgagt catattagca ttgagtacgg
     1921 tttcattttc agcatccaat caaactgact gagatcttca ggatttttga gacttttggc
     1981 tagttcatta gctaaagccc gcaacttatt agatgtcaac tgtttttcat tcataaattc
     2041 accttcattt gatatcaaat taaatagttc aacatcagag aattatacaa acttatgtac
     2101 aggctcctat gatcttcgaa tcctgaagat tatagtgaaa ataagttttc acgttgaaat
     2161 cccacatagt tagtatatga taaataatca tggatgacct gattaacctc ttggggtagt
     2221 gcttttatag aagaataaat attctactaa tttaatacat cacctaaaat agtattgttt
     2281 tttatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata ttccagtgcc
     2341 agatcttact tataataaag gggcttggca gtttggataa actattgttc ttattccact
     2401 aatacaaact ttttgtaaag tatattaaat atacttcatt tcatcctgaa atccccggaa
     2461 gtattcaatc aaacaattac accataaaag gaagaggggg ccctaattaa gttgtcaagc
     2521 atgtttatga tacctgcagg gtataaattt tctggctgca agcagacaca tataaaacgt
     2581 ctttattgat cctacccaca taatatggac acagtcgtaa gtgaggctct ggttttcaaa
     2641 ttgttccgga ctgagaccac cacaccaacg gtgccgtcgc cagcgattgt aatcgcactc
     2701 gatataacta aacactgttg cccgcactat ttcccgatgg ataatagtgt tctccgtgga
     2761 gacattccat tttcagcgaa tgaattttca tctgttgctg tatccagtca tacagtgact
     2821 gcatcagtgg cgcggctctg gcttttcttg ccgccagacg ctgttctgct gaacatcccc
     2881 ggacttctgc ctcgatggca tacagttcac cgatacgctg cagggcttcc gtggtgatgt
     2941 cggttggcac tcttgcatgc acatcgtgga tttttctccg gacatgagcc atacatgcgg
     3001 cttccgttat tctgccggat tcgtacaacg cccggtaacc accgtaagca tcggcctgaa
     3061 gcactccgct gtaaccggcc agatgattct gtggatggat acctttccgg tccggtgagt
     3121 acgcgaacca gaccgccgga ggcatttccg aaccggcgtt acggtcatca cgaacgtaga
     3181 cccacagccg ggcagtcctt gttttaccgc tgcccggctc ccggaccgga acggggatat
     3241 catcggcatg gactttaccg ggcatcagca catactggcg caggatgtca tacagcggtt
     3301 ccagcagttc agcaacagca cctgtccagc gccccagtgt ggcacggctc agctccacgc
     3361 cctgacgacg gtatatttct gactggcggt ataacggcag atagtctgca tattttccag
     3421 cgacaacatg ggccagaagc cccgctccgg cataactgcg tgcaatgggt tttgaaggca
     3481 ccggtgcctg cacgatatgg tcacaccggc aacaggccag tttcggacgt tgtgtttcga
     3541 taaccttaaa ggcgctgctg ataagctcca gctgctctga cacatcacat cccagagaac
     3601 tgagttcgcc accacaggcc gggcagcatt cctcttccgg acggatgacc cgggtttcac
     3661 ggggaagtga ggcaggtaac ggtttacggg ctgaagactg gcgcagggga gatggcagta
     3721 ccgggtcata ttgctcaccc agcgtttccg ccatttcttc ctgaagtgcg ctgattcgct
     3781 cctgtgcttc cagtatctgc cgttcggttt ttgcgcgaag tttttctgag cttttaccga
     3841 actgcatgcg ctgcagtttc gcaaccagcg ccttcagccg gttgatttcg gaagcataag
     3901 ccgccacccg ctgtgagagc aggcggttgt atttcgccat ctggcggatg gtgtcctgtt
     3961 gcgtctgcaa cagtgcccgc aggcgggcat tctcatgaga aagtgaggtg tccatatcct
     4021 cactttacaa cgggttatat gcggatgcca gcgcgttccg ttcgtttcgg gtgtttccag
     4081 tcgatgccct caagaagcat ggataactgg gccggagtaa ggtgcacctt gccgtcacgg
     4141 gtgaccggcc agacgaagcg gccccgctcc aggcgtctgg taaagaggca cagtccgtca
     4201 ctgtcagccc acaacacttt tatctggtca ccccggcgtc cgcggaagat gaacagatgc
     4261 ccggagaacg ggtcatcctt caggacgttc tgaacttttg aggccaggcc gttaaagcca
     4321 tttcgcatat cggtgatacc tgcaaccagc cagatacgcg aacctgcagg gaaagatatc
     4381 atcagtggct gctccctttt atttcgcgga taagtatctg taataacgcc ggcgtcagtt
     4441 tacctttaag cctgagagtt ccggccggca gaaccagctc acagcacaga ctgtcggacg
     4501 gtgtatttat ctgctccggc gttaacgtca cgggaagcag tgcaggcata ttttttccgg
     4561 aaggcagcag gccacctttc cggtattgat ggcgccagtt gaagagcagg ttatcgttga
     4621 ttccgttttc acgggcgatc tgcgccacac aggctccggg ctgcagtgac tgctccacta
     4681 aagcgatttt aaattcataa ggaaaattag gcc
//
LOCUS       DADWTH010000199         4688 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.204, whole genome shotgun sequence.
ACCESSION   DADWTH010000199 DADWTH010000000
VERSION     DADWTH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.204"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(695..1744)
                     /locus_tag="JNY02_004040"
     CDS             complement(695..1744)
                     /locus_tag="JNY02_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBB9435337.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            complement(2463..3422)
                     /locus_tag="JNY02_004041"
     CDS             complement(2463..3422)
                     /locus_tag="JNY02_004041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435338.1"
                     /translation="MSLLLIVMMPSDVFFNAAIWITGSFNYLWPSAIAFIGYSLLIKK
                     INMENHEKTQSLICYLLFVICYLLFVICYFLFFLSSFNEQIAVVNVLLCTTLLIFCKT
                     NNYNTKPLLNAMGISLLVIIYIATCTGNKVRYYAEIEHWFKEYGNFNIIQRSMLGLNL
                     YADMLFSVKSIIPALLAFSSTIICKKKTKILPLISCCILITLYIIHTPPVIFQAIHFS
                     ESNLFSTLSVFRVSFAMILTALIIFPTVISLNFNVTSIFISTMIIGTIATTSMLGLSP
                     SIYASGNRIYFIPYLLLITAIVVSTPIAINNIVANFAKSHYKI"
     gene            complement(3742..4659)
                     /locus_tag="JNY02_004042"
     CDS             complement(3742..4659)
                     /locus_tag="JNY02_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB9435339.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIIFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKDKK"
BASE COUNT         1562 a          833 c          816 g         1477 t
ORIGIN      
        1 ctacaatgat ttcaacttat tttatttaat tgatattacg aaacttaact catacataaa
       61 caatatgact caatactgtc ctaaatagct gcgcagaaaa gtgctccact ttcgctcagc
      121 ccttgctggg cgaagcatca ggaaggcgtt ctcagctagg ttgtgttata tctggcttga
      181 gttttgttgc ccatacgcaa cacattaccc atctgtaaac gacaagtgtg acagaattag
      241 gtgcagctat ttagactata tcgacgcagt aacgataata gatatcgaaa ctgtactaga
      301 tattagctac ttatggttac cggggggagt cttttgagca ctggtgttat caatcggctt
      361 caaataattc accaataaat tatattgcga tagctcatca ccttccaggt tgtttatagc
      421 cagtttatca gcgtcaccac ctactgttta tgcttttatt tctttgatta ataagtatat
      481 gatattttaa ttcatctggt gtatttcttt ctcatgtgtt ggccgactga atatctgttc
      541 agtgaagggg taattttatg tttgcacttc gaacattccc ttcagttgct aggaaagaaa
      601 accgtaacat ttgcgcatta attttaacag tataatctga gcataaatat gcgctatata
      661 tctataaaaa ggcgcaatta attgcgcctt tttatcattt tatcaaaaat ggcccaccga
      721 ctgtcctcga tagccaatgg ccaattttat ctgatacata tacacaaata caggtatata
      781 tgattgcgca gataggattt ggaatggtca cgctgaagcc tatgattgct aaagccatag
      841 ggataaataa tttggtgata tactccattc cacatatgtt aagagtattt cttccagctc
      901 ttacaataaa gtcattccca caaattattt ttgctataaa aatattaaat attatcaaac
      961 ctaccgtggt gataatagta ttaatagctt gaatataact acttgatatt atgtctgcat
     1021 tgaatattga gataatctta aaaagcaaag gtttctggtt aaggagttga tacgcactca
     1081 ttatcgcggt aattgagaag acaatgaaac caattttaga ttttccaaaa aacctgtcac
     1141 gtgttaattc taaaaacata catctaccga gaggcaataa ccaccagtat gccatagcgg
     1201 agtctatgtt ccaaaaccat tgaggatcta gtaatgggtt atgattgagc actgtttgtg
     1261 acaacatgaa cgataatagt gatatgatta aaatcactat attgttcttg actatttctt
     1321 taaagattgc atcaattgct attataacaa aaaggcagtt tataaaccat attgttccta
     1381 caaattggtt gtttctaaca ccatatatta tatcataaat gtggctatat atataatcaa
     1441 tcgtctcacc tgagttaatt gtgtttatta ttaatatagt aaatgcaaaa gtgaaatatg
     1501 gaatcattaa tctgtaaaac tttgacttga tataatcgaa aatagataag tcattttttt
     1561 tgatagtaaa aaaaccagca gcgaaaaaga atagtgggac gtgataacta aaaacaaaag
     1621 gatacaattt gccagccccc aaaccaaggt gacctaaata tatagcaaaa ataccgagaa
     1681 attttaaagc atcaacccaa tcatgccttg tttttctgtt gttgtcgatt ttgttattat
     1741 acatttaaag taggctctcc agttttgtgt gattttcaaa cactcattgc aaatatgaat
     1801 gcctgaaagt ttatgattat taatcaacga gttcaatatt aatttattat ttttcaaagt
     1861 cgcactatat aggcatttac ttccgcagta ccaccgatag tatcgatatc aaatcagcct
     1921 gcggccgcga taactttcgt aacatcctgc cagcggcttt gttgaataaa tcaggttcaa
     1981 gacaatcgtc tctgcatcag gttagagtct caggcaatac acactctttc tggcatagct
     2041 gccactctcg caccatggcc atagccttta cgatctgcat actcaccaga ccagagcccg
     2101 ctccctttcg gggaggaatg agggctctga tattcttttg ccgtagttca tcgtgaaacc
     2161 gtcggatgtc atttggcctg aactaccagt ggtgtaggcc attcaatatc gtattatatt
     2221 gaaatataaa tatgcatcag ctgcacacgg tatatttcca tgccagaaga agtatcctga
     2281 cgtgaaacat gactttgttg tcaggatttc atgagagcgg ttcagcaaaa aatgacgaaa
     2341 atcctagtat agtcgggtga tgagcagtat agaggaatgt gttataacca ataaaagtcc
     2401 aattaaattt gtttttattt ctaagccccc cccaaaaaaa ggaggtggct tttaaattta
     2461 attcaaattt tataatggct cttggcaaaa tttgctacta tgttatttat tgcgattggg
     2521 gttgaaacaa caattgctgt tataagaagc aaatatggaa taaaataaat tctattacca
     2581 gaagcatata tgcttggtga taatcctaac atagatgttg tggcaatggt tccaataatc
     2641 attgtggata tgaaaattga agtgacatta aaattaagtg atattacggt tggaaatata
     2701 atgagtgctg taagtatcat ggcaaaagac accctaaaca cggagagtgt cgagaaaaga
     2761 ttgctttcag aaaaatgtat ggcttgaaaa ataactggcg gtgtatgtat aatatatagt
     2821 gtgatcaaga tacagcaaga aattagaggg agtatttttg tttttttctt gcatataata
     2881 gtactggaaa aagctagtaa tgctgggata attgatttta cagaaaataa catatctgca
     2941 tataagttaa gtccaagcat agatctttgg attatattaa aattaccata ctctttaaac
     3001 caatgttcta tctctgcata atacctgact ttatttcctg tgcaagttgc aatataaata
     3061 ataactaata gtgaaatccc catcgcattt aataatggtt ttgtattgta attgtttgtt
     3121 ttgcaaaaaa ttaacagtgt ggtgcataat aatacattga ctacagctat ctgttcattg
     3181 aatgatgata aaaaaaataa aaaataacaa ataacaaata acaaataaca aataacaaat
     3241 aacaaatagc aaattagaga ttgagttttc tcgtggtttt ccatattgat tttttttata
     3301 agtagagaat aaccaataaa tgcaatagca cttggccaca gatagttgaa tgatcctgtt
     3361 atccaaatcg cggcattgaa aaaaacatcg cttggcatca taacaatcag taataatgac
     3421 atgattgaag tgtttatttt atagtaaagg ttgttttttc tctgaaagat agagcaaatg
     3481 ccaataatta atgatgaaaa cattgctgca ttaattaaag accaagcata aaaatgatta
     3541 ataagattta ttaatgcata ttcaatctat gttcgtgatg accatgtgaa atatctatct
     3601 ataagccact tgatgtgatc cggttctgat attgatttct cggaaaacca aatgtcatct
     3661 gatactagtg tatattgatt taaatgaaga atagttaaac caataaaaaa agcaaaagaa
     3721 agcatatgta atttgattta atcatttctt atccttaata atatatttag gtctattttt
     3781 tacttcaata tatattctgc ctatatattc tccgagaaca ccaatcccga tcagttgcac
     3841 gccacccaag aaaagtattg atacaagcag ggagggatac ccgcgtactg ggttaccaaa
     3901 gacaagggtg tctataatca tccatgcacc atataaaaac gaaatgcttg caacaaacaa
     3961 gcctatataa gtccatacgc gaagaggaaa ggttgaaaaa cttgtgatac cttccagtgc
     4021 cagattccat aatttccagc cattaaattt tgagatgcca gcaacacgct ctgcgcgtac
     4081 atattcaacg acatctgtct gaccacccac ccagctcagt atgcctttca tgaaaagatt
     4141 gcgctcaggc aacagtttaa tgttctccac aacctcacga gacatgagtc gaaaatctcc
     4201 gacattttcc tcgatctttg gggtgcttat tttgttgtgt aacttataga accactcagc
     4261 tgtcttacgt ttcagtcgtc catcagttga gcggtctgaa cgtttcgcaa gcaccatgtc
     4321 agcaccagcc tgccactttt caataagatg agggataacc tcaatagggt cttgcaggtc
     4381 gacatcaata ggaattacag catcgccgct tgcatggtct aaccctgcaa ataaggctgg
     4441 ttctttacca aagttgcgtg taaatgacag cggaacaact agcgggtctg aaacagccag
     4501 cgcgttaata attgactctg tggcatcttt acttccgtca tttatgaata taatttctac
     4561 ttcatatggc ttcaactctt ggaattcacg taccgtctta tagaaaacag gtatcgcttc
     4621 ttcttcattg aagacaggaa cgacaagaga tattttcatt tcgcatccct aaagacaatg
     4681 aactttga
//
LOCUS       DADWTH010000200         4648 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.205, whole genome shotgun sequence.
ACCESSION   DADWTH010000200 DADWTH010000000
VERSION     DADWTH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.205"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            446..1201
                     /locus_tag="JNY02_004043"
     CDS             446..1201
                     /locus_tag="JNY02_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBB9435340.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            1280..1353
                     /locus_tag="JNY02_004044"
     tRNA            1280..1353
                     /locus_tag="JNY02_004044"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1312..1314,aa:Gly,seq:ccc)
     gene            1488..3095
                     /locus_tag="JNY02_004045"
     CDS             1488..3095
                     /locus_tag="JNY02_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB9435341.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPDPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVSKATRADMLRVREVLQLLPKNCKQRFKAMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTVLAAITGARLNEVAQLQVKDVRTTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTMGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVNG"
     gene            3088..3741
                     /locus_tag="JNY02_004046"
     CDS             3088..3741
                     /locus_tag="JNY02_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBB9435342.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
BASE COUNT         1226 a         1109 c         1169 g         1144 t
ORIGIN      
        1 cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc ctcgatttga acatttggtt
       61 cttatggcaa agatttagtc taagcaggca agatttacgc cagaaacgac gataagcgtt
      121 aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga tatgatacga aacaggactc
      181 tgaataggga cagtgatacg tatcaatatg agtggcgata aaatcaatgt gataaatcag
      241 gattattacg catcaggatg ggaaataggg taaaaagtca ggaacttcga tacctctaca
      301 gcgaatattt tgctgatgat acttcccttt gccaaatgaa attaacctct ttctctatta
      361 ccacgttttt ccagaagcaa gagattgggt atcaagaggc tggctgctat gatatggcgt
      421 cttgtttttt aagcgaggaa agattttgag tgcgggacgc ctgaataaaa aatctctggg
      481 tatcgtgatg tttttatcgg ttggactgct attggcgggc tgttcgggta gcaaatcatc
      541 cgatacagga acgtattccg gctccgttta caccgtgaaa cggggggata cgctatatcg
      601 tatttcgcgc accacgggaa ccagcgtaaa agagctggcg cgactgaacg gcatttcccc
      661 cccttacacc attgaagttg gtcagaaact aaaactgggt ggggcgaaaa gtagcagtag
      721 tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca tcggttacac cgtcatcagc
      781 ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt tggttatggc caacgacagg
      841 gaaagttatc atgccgtatt cgacagcaga tggcggcaat aaagggattg atatctcagc
      901 tccacggggt acacctattt acgccgcggg tgcaggaaag gtggtgtatg tgggcaacca
      961 gctgcgtggc tacggtaatc tcatcatgat taaacacagt gaagattaca ttacggctta
     1021 cgcccataat gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg ggcaaaaaat
     1081 cgccactatg gggagcacgg atgcggcatc tgttcgcctg catttccaga ttcgttaccg
     1141 tgcaacggca attgatccgc tacgttactt gccgccgcag ggcagcaagc caaaatgctg
     1201 atggcgaatt aatcagcagt cagcagcgcg gcacttgcta aggagagcgt aaggtttata
     1261 atgccttacg catctcgaag cgggcgtagt tcaatggtag aacgagagct tcccaagctc
     1321 tatacgaggg ttcgattccc ttcgcccgct ccatgacatt tcttaaagtt caataactta
     1381 ctgaatccta agagatatgg cttaacgcct tccgatagtt ttactcactg taaccaacac
     1441 tgtgactaac tttgttgaag tagtgagcag acctaaggtc tcagtacatg ctcaactcaa
     1501 gaacctacct ttatcagcgt aatggtgtct tttacatccg tcttaggatg aagaccactg
     1561 gtcgccttac cgcttcgctt ccttctcaca accgttataa actagcgtca gtatctttac
     1621 ggaccaagga tagacgtacc gctatggctc actcccggca catcaagtca gcacttaaag
     1681 caatccatgc agacaaccct aacgcctctt atgaggagct acgggagcac ctgaagacca
     1741 ttgtcgagtg ggaactcagt gtcagccgcg atgacctgaa cgaccctgag tcctatcagc
     1801 tttacgttga ccagtacgac gatattaagt ccaaccttcg ggaagctgta gcgactgagc
     1861 acctgactgt agaccagcat cgctatatca atgacgttat cggtgtgctt aaagcctgtc
     1921 aggatagact caaaggtgat agctctggtc tgttgtctta ccttgagcct gagactggca
     1981 gtctaagacc gtccgtctcc ctatctgtat tggcagaacc tgaagtccct gaccctaaag
     2041 ctctgaccct cgcgtctctc attgagcagt acgagcagga gaacgcccag aactggaagc
     2101 cagcgaccct gagcgagaac cgagcgtcgc actccacgct aatcgagata ttcgactatc
     2161 tggatattca ggacgtcagc aaggcgaccc gtgcagatat gcttagggtc cgtgaagtcc
     2221 tccaactgtt accgaagaac tgcaaacaac gctttaaggc tatgccgctg tctgacctgc
     2281 tgaaccggga gtctaagact gactgtctgg atgtcgttac catcaacaat aaatacctta
     2341 tcaagatggc tgctgtgttt aagtgggcgg tacgcaacga cctgatagct aagaacctga
     2401 cagaaggtct tgagctgaaa gtcccgcagc gtaaagcctc cgacgctcgt gatgcgttca
     2461 gtcctgagca ggtagggcaa ctactggtcg cagctaaagc gtactcgcag aagacctcag
     2521 ggaagccata ccattattac gtcaccgttc tggctgcaat cactggagcg agacttaacg
     2581 aggtggccca acttcaggtc aaagacgtca ggaccactga ggccgggacc gtgtatatcc
     2641 atatcaacga agacgatagc agcttgccgg gtaagagcat caagaacgcc cacagtgacc
     2701 gctgtgtccc gctggtcgat ggcgcttatg gttttgtatt ggctgacttt atgtcgcttg
     2761 tggaagaccg tagaaagact atgggtgata atgctatggt attcgatggc ctcaagctga
     2821 tgaagaacgg ctatggtgag caggtgagca aatggtttaa ccgaacgttg ctacctaaag
     2881 tccttgctga ccgtagcggc ttagcgtttc actcgtttag acatacagtt gcgacccagc
     2941 taaaacaaca tggggtcgaa ttggcgtatg ctcaggcgat tatgggtcat agctctgggt
     3001 ctataactta cgaccgttac gcgaaagagg tcgaggtaga tagattggtt aatgtgatgg
     3061 cgggtgttta taaggagact ggagtaaatg gctgagtatt atccggcaga gtttggtggc
     3121 actgctttta actgcccgaa ttgtggcgct tattctcaac agcgctgggt ccgcttaaaa
     3181 tatggcgcta acgggattta tgagacacct ttcgcctttt ctaaatgtga acgatgcaat
     3241 gagaaagctt attggtatga cgaaaccatg attataccag cgtcagcaaa cgttgagatg
     3301 cctaacccag acatgccaga cgactgtaag tccgactaca tggaggccag aagcattgtt
     3361 aacctttctc ctaaaggcgc tgccgcactc ctgcgcttaa gtcttcagaa gctgatggtc
     3421 catctcggtg agccggggaa gcatatcgac acggacatta agtcgcttgt agccaaagga
     3481 ttgtctccat tggtccagcg agctgctgat atttgtcgta tcgttggcaa tcaggcggtc
     3541 catccgggtg aaattaatat tgatgacgac cctcagttag cgcatggtct ttttaagctg
     3601 ctgaatatta ttgtcactga gcagattacc agaccgaaag aagtcgaagc catgtttaac
     3661 agcatgccgg aaagggcgct caagggcatt gaggaccggg acaggaaggc gagggaacag
     3721 cagcaggcag ctagcgagta aagctttagg tcagattgaa tataccctca ctatagggaa
     3781 gggggcttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat agcagtcacg
     3841 ccccatacgc tcattcattt tagtctcagg cttccggtag cggaccgatt ggaaaccatc
     3901 cgcagacgcc tagtgttaaa tcaccttggc ctgagaccat tctcctttat cgtagcaatc
     3961 tctctccttg acaggcggag aacatcagac cgcagcggaa gtatccgtct atgcgaccat
     4021 cggtgtgcga gattgtgaag aaccaagaca tcattgctga ggtctgccat ccattgtgga
     4081 caggcggaaa ctcaaagagg ttagaccagt agtggtgtct gaggtttggg ctttgagtcc
     4141 tgaccagcag gctggaggag accatgagtt tatcttgtta tggattgtgt tatcacaatt
     4201 ccaataagaa gaactttaag tccagcctcc agtcctgtca gcccgaagta aactatggtc
     4261 gctatgatgt ggtctccata cttcgggtct aactggaggt cagattatgg ccttatcagg
     4321 tagcaaatct tacaccctca ctgtagagac catacattcc gcctctggtc ttgatgcgtc
     4381 aagttatgcg gaagctctcc cgacattaag tctttctccg aagacatccc aaggcaagac
     4441 agcctctgtc agacctccag cccctagaag ttcttctcct cttgaagtct atcagcttct
     4501 agcatctggt cgtcctgaca cgctgtcgaa ccttcatatc tatggtcatc gcagaccaca
     4561 tcacaattac ctcaagtctt tctgtatgtc tcctgttgcc tgtgagtctc cctatcgttg
     4621 ggggcttgag taggctctca gggtgtga
//
LOCUS       DADWTH010000201         4608 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.206, whole genome shotgun sequence.
ACCESSION   DADWTH010000201 DADWTH010000000
VERSION     DADWTH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.206"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..81
                     /locus_tag="JNY02_004047"
                     /pseudo
     CDS             1..81
                     /locus_tag="JNY02_004047"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin glycosylation ligase domain-containing
                     protein"
     gene            316..1224
                     /locus_tag="JNY02_004048"
                     /pseudo
     CDS             316..1224
                     /locus_tag="JNY02_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
     gene            1224..1958
                     /locus_tag="JNY02_004049"
     CDS             1224..1958
                     /locus_tag="JNY02_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HBB9435343.1"
                     /translation="MVNDLTYKKLQSTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRNERCNNMRTKQKV"
     gene            2426..3187
                     /locus_tag="JNY02_004050"
                     /pseudo
     CDS             2426..3187
                     /locus_tag="JNY02_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            3231..4310
                     /locus_tag="JNY02_004051"
     CDS             3231..4310
                     /locus_tag="JNY02_004051"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBB9435344.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMGYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQHFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..4594
                     /locus_tag="JNY02_004052"
     CDS             4307..4594
                     /locus_tag="JNY02_004052"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBB9435345.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQLLDLPLYFQTSVIT"
BASE COUNT         1262 a          884 c         1126 g         1336 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtgaccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagtgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttacaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggaatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tctgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgtagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt ccgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttacat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggggt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagcatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa agcgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat tgttggattt
     4561 gcccctatat ttccagacat ctgttatcac ttaacccatt acaagccc
//
LOCUS       DADWTH010000202         4549 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.207, whole genome shotgun sequence.
ACCESSION   DADWTH010000202 DADWTH010000000
VERSION     DADWTH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.207"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            132..575
                     /locus_tag="JNY02_004053"
     CDS             132..575
                     /locus_tag="JNY02_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB9435346.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /gene="ybhA"
                     /locus_tag="JNY02_004054"
                     /pseudo
     CDS             811..1630
                     /gene="ybhA"
                     /locus_tag="JNY02_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNY02_004055"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNY02_004055"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBB9435347.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNY02_004056"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNY02_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBB9435348.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNY02_004057"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNY02_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB9435349.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKANEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="JNY02_004058"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="JNY02_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBB9435350.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
BASE COUNT         1233 a         1189 c         1084 g         1043 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgttggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagggtaatg ctgccaactt actgattta
//
LOCUS       DADWTH010000203         4508 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.208, whole genome shotgun sequence.
ACCESSION   DADWTH010000203 DADWTH010000000
VERSION     DADWTH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.208"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            368..2020
                     /locus_tag="JNY02_004059"
     CDS             368..2020
                     /locus_tag="JNY02_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBB9435351.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JNY02_004060"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JNY02_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="JNY02_004061"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="JNY02_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2961)
                     /locus_tag="JNY02_004062"
     CDS             complement(2527..2961)
                     /locus_tag="JNY02_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HBB9435352.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="JNY02_004063"
                     /pseudo
     CDS             complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="JNY02_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1198 a         1090 c         1038 g         1182 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctttcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaacacggg ccagccagtg gctggcccgt
     3241 cgtcggtggc ccctaagtcg cttgtgttta cggggcagcc ataacacgag tttgcgatcg
     3301 atttgcctca gcgtcagata cagtgctgat ttataaaaag caccgtagta ctttatccag
     3361 cctctgattt tcgcattgaa catatgcgcc agctcgtaca aggacttgtc actgcgattc
     3421 tcctcacctc ctgacggatt gcttttcctg ctctggcact catcgccgga ctgaagttaa
     3481 cgaaggtctt accccagcga ttcatcgaca gacgcggccg gaatgtatag cccagaaagt
     3541 tgaagctcgt ttccgggtag tccccccgtc ggtcagcatc cttacagtaa actattcgtg
     3601 ttttttgcgg atgcagctgc agcccgcatt gcgcaagccg ctgcctcagt ccacttatca
     3661 gagcgtcagc ctgccactgg ctgtgacagt ggcatacaac atcatcggcg tacctttcga
     3721 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3781 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3841 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3901 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaaaga
     3961 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     4021 caatcgcctg gtgtgaggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     4081 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     4141 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     4201 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     4261 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     4321 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     4381 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     4441 ggggcaagcc ctttgcttcg gacccattac ggtccttcac cgctactacg gtttactccg
     4501 tccctgta
//
LOCUS       DADWTH010000204         4439 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.210, whole genome shotgun sequence.
ACCESSION   DADWTH010000204 DADWTH010000000
VERSION     DADWTH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.210"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..213)
                     /locus_tag="JNY02_004064"
     CDS             complement(<1..213)
                     /locus_tag="JNY02_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBB9435353.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="JNY02_004065"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="JNY02_004065"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBB9435354.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLGGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="JNY02_004066"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="JNY02_004066"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBB9435355.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="JNY02_004067"
     CDS             2894..3634
                     /locus_tag="JNY02_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB9435356.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="JNY02_004068"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="JNY02_004068"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBB9435357.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..>4439)
                     /locus_tag="JNY02_004069"
     CDS             complement(4192..>4439)
                     /locus_tag="JNY02_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435358.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1099 a         1105 c         1233 g         1002 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctgggagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgatgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tctggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttg
//
LOCUS       DADWTH010000205         4420 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.211, whole genome shotgun sequence.
ACCESSION   DADWTH010000205 DADWTH010000000
VERSION     DADWTH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.211"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..446)
                     /locus_tag="JNY02_004070"
     CDS             complement(<1..446)
                     /locus_tag="JNY02_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBB9435359.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLEL"
     gene            complement(561..636)
                     /locus_tag="JNY02_004071"
     tRNA            complement(561..636)
                     /locus_tag="JNY02_004071"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Thr,seq:ggt)
     gene            complement(643..717)
                     /locus_tag="JNY02_004072"
     tRNA            complement(643..717)
                     /locus_tag="JNY02_004072"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(682..684),aa:Gly,seq:tcc)
     gene            complement(834..918)
                     /locus_tag="JNY02_004073"
     tRNA            complement(834..918)
                     /locus_tag="JNY02_004073"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(882..884),aa:Tyr,seq:gta)
     gene            complement(927..1002)
                     /locus_tag="JNY02_004074"
     tRNA            complement(927..1002)
                     /locus_tag="JNY02_004074"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(967..969),aa:Thr,seq:tgt)
     gene            1364..2314
                     /gene="coaA"
                     /locus_tag="JNY02_004075"
                     /gene_synonym="panK"
     CDS             1364..2314
                     /gene="coaA"
                     /locus_tag="JNY02_004075"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBB9435360.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2343..3308)
                     /gene="birA"
                     /locus_tag="JNY02_004076"
     CDS             complement(2343..3308)
                     /gene="birA"
                     /locus_tag="JNY02_004076"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBB9435361.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3305..4333)
                     /gene="murB"
                     /locus_tag="JNY02_004077"
     CDS             complement(3305..4333)
                     /gene="murB"
                     /locus_tag="JNY02_004077"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBB9435362.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1128 a         1108 c          999 g         1185 t
ORIGIN      
        1 agttccagca gctcttcgtc atcaaccatg tcgcatttgt tcaggaacac gatgatgtac
       61 ggaacgccta cctgacgccc cagcaggatg tgctcacgag tctgcggcat cgggccgtca
      121 gtcgcagcaa ctaccaggat cgcgccgtcc atctgagcag caccggtgat catgttttta
      181 acatagtcgg cgtgccccgg gcagtctacg tgtgcgtagt gacgggtcgg ggtgtcgtat
      241 tcaacgtgag aagtgttgat ggtgatacca cgagcttttt cttccggcgc gttatcgatc
      301 tggtcgaatg cgcgagcagc accgccgtag gttttagcca gtacggtagt gattgcagcg
      361 gtcagcgttg ttttaccatg gtcaacgtgg ccgatagtac cgacgttaac gtgcggtttt
      421 gtacgttcaa acttttcttt agacatcgat tgtccctcta agacacggat aaatcggtga
      481 tatcaccaca tcaaccaggc aacatgcccg acttgttgaa tgcaataaac agaaggaaaa
      541 aacagggagg agaaaagaag tggtgctgat aggcagattc gaactgccga cctcaccctt
      601 accaagggta cgctctacca actgagctat atcagcacat cttggagcgg gcagcgggaa
      661 tcgaacccgc atcatcagct tggaaggctg aggtaatagc cattatacga tgcccgcatc
      721 ctagaactcg gctacctgat tttcattctg cactgaatat cgagagaagc tctctttatt
      781 cgagccggta agtgaactta tcgtctcggg ctacgccatc gcgtggccga aattggtggt
      841 gggggaagga ttcgaacctt cgaagtctgt gacggcagat ttacagtctg ctccctttgg
      901 ccgctcggga accccaccgg acttgatggt gccgactacc ggaatcgaac tggtgaccta
      961 ctgattacaa gtcagttgct ctacctactg agctaagtcg gcatcaagta gcgcgcattc
     1021 tatggagaca tgcgagttca tgcaactaaa aaattgcaca atttgtttta ttggtcacat
     1081 tttatgctac acgatgaaga aacagccgaa cggagggcag attagcacac tttttcaaca
     1141 tcattgtgct caacaatgcg ctcctgctaa accataattc tttttatcag atggaatatc
     1201 tgtcacattg cttttcaacg atagcttcct ggcagagatt ttttcttatt attcctcccc
     1261 atctggtgtt accctcctgc ccattaaccc attcaacaga actgtgacgc gccatggcaa
     1321 atatcgcttt gccgatagag ctatgaccgc cagaaacatg cttatgagta taaaagagca
     1381 aacgttaatg acgccttacc tacagtttga ccgcaaccag tgggcagctc tgcgtgattc
     1441 cgtacctatg acgttatcgg aagatgagat cgcccgtctc aaaggtatta atgaagatct
     1501 ctcgttagaa gaagttgccg agatctattt acctttgtca cgtttgctga acttctatat
     1561 aagctcgaat ctgcgccgtc aggcagttct ggaacagttt cttggtacca acgggcaacg
     1621 cattccttac attatcagta ttgctggcag tgtcgcggtg gggaaaagta caaccgcccg
     1681 tgtattgcag gcgctattaa gccgttggcc ggaacatcgt cgtgttgaac tgatcactac
     1741 agatggcttc cttcacccta atcaggttct gaaagaacgt ggtctgatga agaagaaagg
     1801 cttcccggaa tcgtatgata tgcatcgcct ggtgaagttt gtttccgatc tcaaatccgg
     1861 cgtgccaaac gttacagccc ctgtttactc gcatcttatt tatgatgtga tcccggatgg
     1921 agataaaact gttgttcagc ctgatatttt aattcttgaa gggttaaatg tcttacagag
     1981 cgggatggat tatccacacg atccacatca tgtatttgtt tccgattttg tcgatttttc
     2041 gatatatgtt gatgcaccgg aagacttact tcagacgtgg tatatcaacc gttttctgaa
     2101 attccgcgaa ggggctttta cagacccgga ttcctatttt cataactacg cgaaattaac
     2161 taaagaagaa gcgattaaga ctgccatgac attgtggaaa gagatcaact ggctgaactt
     2221 aaagcaaaat attctaccta ctcgtgagcg cgccagttta atcctgacga aaagtgctaa
     2281 tcatgtggta gaagaggtca gactacgcaa ataatttgca ggggagcgaa tactcccctt
     2341 tcttattttt ctgcactacg cagggatatt tcaccgccca tccagggttt tattattcca
     2401 tcctgctcaa gtaataaagc cccctgtttg tctattccgc gtgaaatgcc aaatatttct
     2461 ttatcaccaa tgataagttt cactgggcga ttaataaaat tatccagctt ttcccagcgc
     2521 gacagataag gtgccaatcc ttcttgttcg aagagttcca acgcagcacg taattcacgt
     2581 attagcatgg ccgccaacgt attacgatca agattgatcc ccgcttcctg cagcgtgatc
     2641 cacccctgat taacgacact ctcttcaaca cggcgcattg ccatgttgat cccggatcca
     2701 atgactattt gcgccgcatc gccagttttg ccagtcagct ccacaagaat gcctgccagc
     2761 ttgcgatcct gcagatagag gtcattaggc catttgacac gaactttatc tgctcccagc
     2821 tttcgtaata cttccgccat cacgataccg ataaccagac ttaaaccaat cgccgccgcc
     2881 gggccttgtt ccagacgcca gaacatcgac aaatataagt ttgcgccaaa aggcgaaaac
     2941 catttccgcc cccggcgacc acggccagcc tgctggtatt ctgcaacaca ggcatcgccc
     3001 gatttaagct ctccgatacg atcaagaagg aactgattcg tggagtcaat aactggcagc
     3061 acggctacac taccgccatc cagctgaccc aatatctgtt cagcattaag taactggatg
     3121 ggctcaggca ggctgtatcc tttacccgga acggtaaaga catcaacgcc ccagtcacgc
     3181 agtgtctgaa tgtgtttatt aatagccgcc cggctcattc ccagcgtttc acccaactgc
     3241 tcgccagagt gaaattcacc gttcgctaac agggcaatca atttcagtgg cacggtgtta
     3301 tccttcatga aattgtctcc actgcgctca cttcacctga tgcaccaata aagcggactt
     3361 caggctcaag ccagacatta aatttttcac ccactttttg tcttacatgg tgtgccagtt
     3421 gcaccacatc ttcgcttttt gcattgtctt cattaatgag aactaacgcc tgttgacggt
     3481 gcaccgcagc cccacccatt tgcatccctt ttagctggca ctgatcaata agccaacctg
     3541 ctgccagttt tactgaacca cccgcctggg ggtaatttgg tgctgttgga aattgcgcca
     3601 gtaatgcttt agccgtttcg gcagatacaa cagggttttt gaagaaacta ccggcattgc
     3661 cattcacttt tggatcaggg agtttggtgg tgcgcatatg acacaccgca ttaaatactt
     3721 gctgtggcgt tactgttgta ggatccagac gagttaagtc accatacgtt agtacaggtt
     3781 gccactcttt tggcagacgc agacctacgg ctacaatggc gaagcgatcc tggtattcat
     3841 gtttaaaaat actgtcgcga tagccaaaac ggcactcttt ggcagttaag cgcacttgct
     3901 tgcctgtcgc cagttcaaca caatcaacat aagcgcaaac tcgctgtaat tctacgccat
     3961 aagcaccaat attctggata ggtgatgagc cgacacaacc aggaattaat gccagatttt
     4021 ccagaccagg cataccttcc tgcaaagtgt attttaccag gcgatgccag ttttctccgg
     4081 ctcctacatg taaataccac gcatcaggtt catcatgaat ttcgatacct ttgatccggt
     4141 tgatgatcac cgtgccgcga tagtcttcca gaaaaagtac attacttcct tcacccagaa
     4201 taagaacggg ttgtccttct gcggttgcat gctgccaggc attgagtaat tgttgttcgt
     4261 cttcggcaca tacaatgtgc tgagcattat gatcaatgcc aaatgtgttc cagggtttta
     4321 aggagtggtt catagctgct ttcctgatgc aaaaacgagg ctagtttacc gtatctgtgg
     4381 ggggatggct tgtagatatg acgacaggaa gagtttgtag
//
LOCUS       DADWTH010000206         4380 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.212, whole genome shotgun sequence.
ACCESSION   DADWTH010000206 DADWTH010000000
VERSION     DADWTH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.212"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(64..822)
                     /locus_tag="JNY02_004078"
     CDS             complement(64..822)
                     /locus_tag="JNY02_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB9435363.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQYFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JNY02_004079"
     CDS             complement(830..1933)
                     /locus_tag="JNY02_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB9435364.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="JNY02_004080"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="JNY02_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="JNY02_004081"
     CDS             complement(3181..4206)
                     /locus_tag="JNY02_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB9435365.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1215 a         1147 c         1023 g          995 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatactg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
//
LOCUS       DADWTH010000207         4327 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.213, whole genome shotgun sequence.
ACCESSION   DADWTH010000207 DADWTH010000000
VERSION     DADWTH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.213"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..157)
                     /locus_tag="JNY02_004082"
     CDS             complement(<1..157)
                     /locus_tag="JNY02_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435366.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNY02_004083"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNY02_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBB9435367.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="JNY02_004084"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="JNY02_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBB9435368.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATKTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNY02_004085"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNY02_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBB9435369.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..>4327)
                     /locus_tag="JNY02_004086"
     CDS             complement(3800..>4327)
                     /locus_tag="JNY02_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBB9435370.1"
                     /translation="RTTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
BASE COUNT         1143 a         1083 c          994 g         1107 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg tttttgtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacgacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatgcca cgaaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcgcttct gctctgccag
     4321 tggtgcg
//
LOCUS       DADWTH010000208         4326 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.214, whole genome shotgun sequence.
ACCESSION   DADWTH010000208 DADWTH010000000
VERSION     DADWTH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.214"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..324
                     /locus_tag="JNY02_004087"
     CDS             <1..324
                     /locus_tag="JNY02_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBB9435371.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="JNY02_004088"
     CDS             383..586
                     /locus_tag="JNY02_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HBB9435372.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRELFTCNKTLPPHEVEPNFGAI"
     gene            586..1623
                     /locus_tag="JNY02_004089"
     CDS             586..1623
                     /locus_tag="JNY02_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB9435373.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPSNSKCDFCH"
     gene            complement(1651..1740)
                     /locus_tag="JNY02_004090"
     tRNA            complement(1651..1740)
                     /locus_tag="JNY02_004090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1704..1706),aa:Ser,seq:cga)
     gene            1834..2631
                     /gene="mtfA"
                     /locus_tag="JNY02_004091"
     CDS             1834..2631
                     /gene="mtfA"
                     /locus_tag="JNY02_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBB9435374.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2732..2807
                     /locus_tag="JNY02_004092"
     tRNA            2732..2807
                     /locus_tag="JNY02_004092"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2765..2767,aa:Asn,seq:gtt)
     gene            2969..4231
                     /locus_tag="JNY02_004093"
     CDS             2969..4231
                     /locus_tag="JNY02_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB9435375.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGSELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1206 a          996 c         1036 g         1088 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gagctattta cctgcaataa
      541 aactttacca ccacacgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagtaatt ccaagtgtga tttttgtcac
     1621 tgacttaatg atgtataagt gattgaattt tggcggagag agggggattt gaacccccgg
     1681 tggaggtgcc cccactccgg ttttcgagac cggtccgttc agccgctccg gcatctctcc
     1741 gctcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct gtccgtgcaa
     1801 ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg gaaagtacaa
     1861 gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc ccttttaacg
     1921 tgtctgacag aacaggaaca aagcaaattg gtcgctcttg ccgaacgttt tttacagcaa
     1981 aaacggcttg ttcctttaca gggctttgag ctgaattcat taagaagctg ccggatagca
     2041 cttctatttt gcctgcccgt tctggagtta ggactggaat ggctggatgg ttttcatgaa
     2101 gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga tatcggtctg
     2161 gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc tatcgttttg
     2221 aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat tattcatgaa
     2281 gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc ctttatttcg
     2341 ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa cattcaggaa
     2401 gaaatcgaat tagttggtga gaatgcggcg agcattgatg cttatgctgc cagtgatcct
     2461 gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact ttttgctcct
     2521 cgtttccctt cattgtggca acgtttctgt caattttatc aacaagatcc tttgcagaga
     2581 ctgcatcacg ctaatgatac agactcgttt tcggcgacga atgttcatta attaacaact
     2641 ttgcagatta attaaccaat tgaaatggct tatgaaattt agtgttgaca gacaaggtac
     2701 cgctaagtaa tatgcgcccc gttctcacga ttcctctgta gttcagtcgg tagaacggcg
     2761 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaatt ttctgttttc
     2821 atgcatcctt gcgaatcttt atctgatgtt gattcaacag gttagtgtga aaactcttct
     2881 cggtgcattt tgattttacc ctacgcatcg ggaaaaattg gtggtcaaat ctgggatcag
     2941 gttagttcga taatggagtg acccccatat atcccttacc gacgcaaaaa tccgcaccct
     3001 caagccttct gataaaccct ttaaagtctc cgattctcac ggtctgtatc tgctggtcaa
     3061 gccgggtggc tcccgccact ggtatctcaa ataccgtatt agcggtaaag aatcccgcat
     3121 tgcgctgggt gcctatccag ccatctccct gtctgatgcg cgacagcaac gtgaaggtat
     3181 ccgtaaaatg ctggcgctga atatcaaccc ggtacagcag cgggctgctg aacgtggctc
     3241 acgaacaccg gagaaagttt ttaaaaacgt ggcgctggcg tggcataaaa gtaacaggaa
     3301 atggtcgcag aacaccgccg accgtctgct tgccagcctg aacaatcaca tctttccggt
     3361 catcgggaac ctacctgtat cagaacttaa accccgtcat ttcattgacc tgctgaaagg
     3421 gatcgaggaa aaaggtctgc tggaggttgc gtcccgcaca cggcagcacc tgagtaacat
     3481 aatgcgccat gcggtccatc tggagttaat cgatacgaac cctgcagcaa accttggcgg
     3541 cgtgaccaca cctcctgtca gacggcacta tcctgccctg ccgctggagc ggctgcctga
     3601 actgcttgaa cgtattgggg catatcatca gggcagtgaa ctgacccggc atgccgttct
     3661 gctgatgctg catgtgttca ttcgctccag tgaactgcgt ttcgcccgct ggtcagagat
     3721 tgatttcaca aaccgagtct ggacgatacc cgcgacgcga gaacccatta ttggcgtgcg
     3781 ttattccggc cgcggggcaa aaatgcgaat gccgcatatc gtccccctct cagaacagtc
     3841 catcgccatt ctgaaacaga ttaaggatat caccggtaat aatgaactga tcttccccgg
     3901 cgaccataac ccgtataagc caatgtgtga aaacacggtc aataaggcac tgcgggtgat
     3961 gggttacgac acgaaaaagg atatctgcgg tcacggcttc cgggcaatgg catgcagtgc
     4021 gctgatggaa tcgggtttat gggcaaagga cgcagtagaa cgccagatga gtcatcagga
     4081 gcgcaatacc gtgcgcatgg cttatattca taaggcagag cacctggaag cccgcaaagc
     4141 gatgatgcag tggtggtcgg attatctgga agcatgccga gaatcttatg caccgcctta
     4201 tacaattggt aaaaataagt ttatcccata gtatactaac gaatcccaca ctctggcaaa
     4261 ttaccaccac ctctacagga ggtggttcta tatagacttt tatcaacttt atgacgaaaa
     4321 acacct
//
LOCUS       DADWTH010000209         4299 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.215, whole genome shotgun sequence.
ACCESSION   DADWTH010000209 DADWTH010000000
VERSION     DADWTH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.215"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..300)
                     /locus_tag="JNY02_004094"
     CDS             complement(<1..300)
                     /locus_tag="JNY02_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435376.1"
                     /translation="MTDITANVIVSMSSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQ"
     gene            complement(432..2273)
                     /locus_tag="JNY02_004095"
     CDS             complement(432..2273)
                     /locus_tag="JNY02_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBB9435377.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2273..3679)
                     /locus_tag="JNY02_004096"
     CDS             complement(2273..3679)
                     /locus_tag="JNY02_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBB9435378.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQENQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVGPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(3689..>4299)
                     /locus_tag="JNY02_004097"
     CDS             complement(3689..>4299)
                     /locus_tag="JNY02_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBB9435379.1"
                     /translation="NTLQETRGGSQKYAADLQNQQWQTIMKNLTPFTPLAEQYVNQLQ
                     NLSSLEGQGQALNQYYNSQQYKDLAGQARYQSLAAAEATGGLGSTATSNQLATIAPTL
                     GQSWLSNQMSNYNNLANVGLGALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSL
                     QTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          989 a         1094 c          966 g         1250 t
ORIGIN      
        1 ctgttgcgaa ccatacgcat catatacagc cattgaatgg ccttgctcag ttacaaattt
       61 ggcaatctgc ccgttgtaca caggatatcc ggcagcattg ataacaattg gctgagaaac
      121 agggacgtga gatccgtctt cattctctac ataaacctga atctggtttt caggatttac
      181 cgggtcagtg tcaatttttc cgatataaat tttgccattg gctacggctt taaaagaacg
      241 agccatagtg aagagttgcg aagacatcga tacgatcaca ttggctgtaa tgtctgtcat
      301 ttaatttgct ccagatacaa tgaatcgccg cagcgtggct acggtgaatt ttgggcataa
      361 aaaaacccag ccgaagctgg gtagttgcgt tggttatctg tcagtagtta tgtgcttatt
      421 taattattta tctattgttc tgctgtaatt tggcttgaaa gtatagggcg tatcgcattt
      481 gcagcattat ttaacgctct ttcataagct gccgttcctg ctttggtgtt tgccaaacga
      541 agaagcatat tccttgcggc tttggactca tacaaacgca tcattgcacc aaaaccagcc
      601 tcaagcccca ttgatacacc aagagtcgca gttgcgccaa tcgtccttat cctgttggct
      661 tgcgattgcc ccgtctgagt tactacattt gcggtgtctg accttgctgt ttgctgtaga
      721 acttcatgaa gagcatcaag ctctttcatg tgctttccag aaaaaatagt gttgtaaatt
      781 tcaccgcctg actgagattt cagcttatta acttcagtga tgaacttggc tggagagtca
      841 ccggcctttt ccgctatttt gctgacgtaa gctgcacgca tagcatcttt ccctttatca
      901 tccagtgcgc tccagattcg tttcacgtca gatggttttc tgcttaatac aacggtattt
      961 ataagttcag gactggcttc actgcttgcc ttgttgagct tgttagcaat gtttttatta
     1021 agcaccttat tataaacgtt tgcataatcg gaatttgctt taaggtattt tgctgcgtct
     1081 gatgcaccga ggtttttagc aactgcgtta cgaaggtctt ttgacattgc attctctacc
     1141 atattggtag ctgcttttgc ctggttgggg aagaccatag catctccctg aacattagat
     1201 ctaaatgctg ttctgtgctg acgcaagaga tcaaacgtaa catccaaatc agttgcaggg
     1261 tttgctaatt cttcacgtag gttacgcaag gatgtaagca ggctttgatt ggcagacgtc
     1321 ccaagccgtt cctgtcttgc gatcgctgta ttcagagcat tcatggtatt tgtggtatca
     1381 actgcggcat tacccatttt attggtgacg tcattgataa cagcgccagc ggcatccttc
     1441 cgccccctta acgtggtggt aagagatttc accacatcat cagggttgta ctcaccaaaa
     1501 cggtcaaaat aattacttac cagcttacta cgcgttgcat attgctccgc tcgctttgag
     1561 cctgtcccga gcaaagcccc ctcggcatcc tgagttaggc cgcgagtgaa agcatttttc
     1621 ggcgggataa catcagatgt cattggtgtc acgcccatcg attctgatgt ggcaattttc
     1681 ttcgccactt ctggcgcaat atcacctttt atagccgtta ttccacgccc tattcccttt
     1741 gctgctgcgg aaagaacacc ctgagcggca aggttaactc cggcattttt agctgcattt
     1801 tgtgcgaaat cgcctttctg atttgcggcc tctgccagtg atccaatagc catgcttcct
     1861 gccgttccaa ctcctggaac taaataccca ccaattgttt caccggcttg cgcgtagggg
     1921 tctgtcggtc tgtctactgg acgataaaca tcatccaaaa ccttgggtcc accaagcccc
     1981 tgactgattg cattaatcag acttgcgccg ccctgcaata cgtcaaatgg tatgtttacc
     2041 agaccacgac cagcctgttc tgcaatttgc cctgcacttt gaccaccagt gagccaatcg
     2101 ccagcttgtt gcatcaatga tggttcttcc cgtgttggtg cattattggc ctgattaact
     2161 gtttgttgct gaacagcctg accagcaaaa tactcatcaa tggcggtgcc aatatcttcg
     2221 gtgctcgtac catcaggaaa ggtaaatgtc ttaccgtttg cagttacttt catcattcca
     2281 ccgtaaattg aatgcctgat tttgaggtat atgatccaac ctgattccgt ggttctcctg
     2341 aaggtgtcga atcttgtgct ggcgctgcgt cagtattcat tgacatatac cgcttaacgg
     2401 cactccccaa tgattcacct tttttaacat ccaaccccaa tatctgaccg ccattacgcg
     2461 attgtccagg gttgccattc gcgctcatcc actcggcttt aaactcatta aactgcgcgt
     2521 ttcgtcgctc aaggtttgcc attgcatcaa gccatcttgc gaccgtctca gggttatcca
     2581 tgtcagttgg cgcaccctgt cgaacgatct caacgtcttt atccgttgct gggccgggag
     2641 gtaggaattt aagaacctga ctgttaacaa gggcattttg gcgaatgcgc aaatcacgca
     2701 atgttgtatc gcttccggta agttttgcga acatgttctg tgcgttaccg aacaaacctg
     2761 tcgttggttt ttctgctctg aactgttgag caagcgcact catagaattg gctgagtttg
     2821 atgatgctgt agcattgttt acagccgtct cgatgccttt ttccatgttt actgacagct
     2881 taggtgcttc gctaatcaac tgctgagcct tttcctgcgc ttgctgcatc ttaaacccga
     2941 actcttgctg atccagagcc aagcgttgtg ctgcgatatt gtgcccagtc attgctgact
     3001 gataggaaag gttttgccct ctcgcctgaa gtgcttctcc agcctgattg ctgcggattg
     3061 tctctgccag cctgcctcgg tcaatttcac gaccagccat cttgtcctga acattgaagt
     3121 aatcaatcgg accaagagca gccattccaa ggtgatcaac aaactcacca aatcctgaag
     3181 gattctgctg atacatctga gcaacgctgt tagggccaac accgacgcgc gccagctcat
     3241 tggcgttgtt ttgcagccat gattgcattg cttctggaga cgaggccgca aggcgtgcgc
     3301 cagccgctaa ggtgccgata gaattacgct ggtcttcatc aatgaatccc atgcctttac
     3361 gaacggattc aatctggtct ggatattgag tagccaactg acgcaaagca ccgcgatcac
     3421 cagacgcata agcattagcg tacgcctgct gaaattcttt ctgccgctga gcctgatttt
     3481 cctgctgaaa cacccctgca atacctgaaa ggccttgcaa agcagtcagc ccaacattgt
     3541 tagcgcctga acgctcaata tcattgttct gcctgataag ctgaagcgta ttgccgatgt
     3601 catttacgct cggagcgttt gagttgacgc cgccgatacc agctaacaat ccgccatttg
     3661 atccttgcca agtagccatg attacccctt aaaacaacga gccaagcaat ccgataccag
     3721 caccaatgcc agcgccccaa ggtgttgatg ttcccaaaag gctggcaaga cctgcaccgg
     3781 caatcgcacc agatgttccg ccactaattg cagtctgaag acttgatggt ttattggcat
     3841 tagcagcggc aagagctgcg ctttgctgtg caatgctgct catgttgttg gcgtacgtct
     3901 gcccggcgtt tgcctgacct tgcagcgcac caagcccaac gtttgccaga ttgttgtaat
     3961 tgctcatctg gtttgataac caagactgac cgagtgtcgg cgcgatcgta gccagttgat
     4021 tgcttgtggc tgtcgaacca agtccacccg tcgcctccgc agcagcaaga ctctggtaac
     4081 gagcctgacc tgcaaggtct ttatactgct gagagttgta atactgatta agtgcctgcc
     4141 cctgaccttc taaactggaa aggttctgaa gctggttaac atactgctcc gcaagaggcg
     4201 tgaacggagt aaggtttttc atgatcgtct gccactgctg attttgcagg tctgcggcat
     4261 acttctgtga accgccccgg gtttcctgga gagtgtttt
//
LOCUS       DADWTH010000210         4295 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.216, whole genome shotgun sequence.
ACCESSION   DADWTH010000210 DADWTH010000000
VERSION     DADWTH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.216"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            80..521
                     /gene="tnpA"
                     /locus_tag="JNY02_004098"
                     /pseudo
     CDS             80..521
                     /gene="tnpA"
                     /locus_tag="JNY02_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(639..1583)
                     /gene="hypT"
                     /locus_tag="JNY02_004099"
                     /gene_synonym="qseD"
     CDS             complement(639..1583)
                     /gene="hypT"
                     /locus_tag="JNY02_004099"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBB9435380.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQEAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTPPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1928..2647
                     /locus_tag="JNY02_004100"
     CDS             1928..2647
                     /locus_tag="JNY02_004100"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBB9435381.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2713..4011
                     /locus_tag="JNY02_004101"
     CDS             2713..4011
                     /locus_tag="JNY02_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBB9435382.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1078 a          931 c         1057 g         1229 t
ORIGIN      
        1 ggggaaaaac gagtattttt agtcatcctg tttacctctt tctcaggaag tttagtctcc
       61 aggattcccg gggcggttca tagcgcacac ccgatggaac tgtaaatatc acatagtttt
      121 ttgcgccaaa ataccgaaga caggtgttct acagagagaa gcgtagagca acaggcagta
      181 ttttgagaaa gctgtgtgag tggaaaagcg tacggattct ggaagctgaa tgctgtgcag
      241 atcatatcca tatgcttgtg gagatcccgc ccaaaatgag cgtatccggc tttatgggat
      301 atctgaaagg gaaaagcagt ctgatgcttt acgagcagtt tggtgatttg aaattcaaat
      361 acaggaacag ggagttctgg tgcagggggg actacgtcga tacagtgggt aagaacacgg
      421 cgaagataca ggattacata aagcaccagc ttgaagagga taaaatggga gagcagttat
      481 cgattccctg tccgggcagc ccgtttacgg accgtaagta acgaagttgg atgcaaatgt
      541 cagatcgtgt gcgcctgtta gggcgcggct ggtaagagag ccttataggc gcatttgaaa
      601 aacctccggc tatgccggag gatatttatt gcacgtcgtc aattgcggcg tgctggctct
      661 tccattgata tctgctggat taaatcgtct ggcatatgtt cctgtaaaat acgccagagg
      721 ttctcggcgg ttctattcag tcgggtgttc attcggtaaa tataagcctg aatggggact
      781 gtcaatttag cagcatcaag gcatatcaga cgtttggtct gtagttcatc gacgatcgac
      841 cagatcggta accaggcaat gccgtatccg ttgagcgcca tattctttaa taactcgctc
      901 atcgaagaga taaatatcgt gcgcccggta atacctccaa cctctgccag atgacgattg
      961 accaacctgc ccatataaga tgtggcggta tagttgagca aaggaacctg gggctgagtg
     1021 atatcgaata cagggtgccc ctgtgcgtcg gcggcacata ccgggtacaa ctcagattca
     1081 aacagtttca ggcagcaaaa aggcgattgc ataagatctt catcgcgaaa cgaaataata
     1141 aagtcactct tgccttcgcg taacgtattt acggcctgga caacatcaat ggcttcaaca
     1201 tgatagacaa attctccgcc ataggcggta agtgaatgca ccagtttggg taatacgctc
     1261 agggatagtg agtgcgcggc tgcaatttta atgttgggaa cgccgagtaa actctggccg
     1321 tttaactccc ccaaattgca ttcaagctgc tgtaataagc tgcgagtctg ggaatgaaag
     1381 agcttgccct cttcggttaa ttgtaaaggg ggggtcgtgc gatcgaataa aacaacgccg
     1441 atcgctgcct ccagcgcctt aattcgtcgg ctgaatgcgg gttgcgaaag attacgctct
     1501 tttgcctcct gagaaaaatg gcgacaggct tccagtgtca ggaaatcgta taaccatttt
     1561 gtctcgatat tcttcattac catggcacct gtattttcaa tccaaagaca taataaaaca
     1621 tcccggcaat aaaaataatg ttctcaatta gcacaacctg gcctttatca ttcgttttgt
     1681 gcataacccc ttcatttccg gtattgacgt tgatatcaca tttcgccaaa aaaacgaaat
     1741 ttttccgtca gataaccaga atacatcatc actatggtga ataagcggaa tgttccatag
     1801 ctgcatcatg cgtgtcttgc atcgccttgt acaaccttgc atttctcatc gcccatttcg
     1861 actgttaaaa aagagagcag cagttattta ccgcgtattg tcattaacgt tttgagaggc
     1921 aggtattgtg aaaggtttaa ttggtgtact cggtggaatg gggccggcag ctaccgtcga
     1981 cctgtttaat aaatttgtaa cttttaccgc tgcacaatgt gatcaggaac atattccttt
     2041 aattatttct tcgatcccgg atattcctga ccgtaccgat gcattaatgc atcatgggcg
     2101 ttcaccatta cccgttatgc gtgattatat gcataagctg gaagatgccg gggcagaatg
     2161 tattgttatt ccctgtaata ccgcgcatta ctggttcaaa gaattaaaag atgcttgtca
     2221 tattgatata ttaagtatcg tagaaactac gatcaatgaa gttcgcgcct gtggtaaaac
     2281 ccgaattggc ttgttagcca ccaatgccac gctatatatg ggtttatacc aaaaaggaat
     2341 tgagtcgctg ggatttacct gtgtcagccc cgatgccgac ggtcagaaaa aagtgatgga
     2401 aagtatttat tccttgaaag ctggcaatat agcgcacgca caaaagttga tgaatgagca
     2461 ggctgaaata ttattttccc gcggtgcaga aatccttgtt cttggttgta cagaaattcc
     2521 ggttattctg gcgcaagccg ttaaagagca gccttcccgc tatattgact caacggcgtc
     2581 actcgttcgt gccggaataa aatggtacga aaatcgtgtc ggtaaacatt atcttttaac
     2641 ccaataatca tttaaatcgc agccagaaag ttattcgctt ttaactgaat tatatttata
     2701 acggagaaca ttatggtttg gctggaaatt atcgtagtac ttggtgcaat attttttggt
     2761 attcgccagg ggggaatcgg tattggttta tgtggcgggc ttgggcttgc cattctgact
     2821 ctgggatttg gtctgcctat ggggtcgccg ccagtcgacg tgatcctgat tattatgacc
     2881 gttgttgtgg cggcctcagc cttacaggcc gcaggcggaa tggattatct ggtacgcgtc
     2941 gctggtaatt ttatgcggcg taacccgaaa tatatcaaca taatcgcgcc gattatcacc
     3001 tggtctatga ccattatggc cggtactggg tttattgttt tttccacgct gccggtgatt
     3061 gctgaagtgg cgaaagagtc cggtatccgt ccttcccgca cgcttgccgg ttcggttgtc
     3121 gcttcgcagg ttgcgatttc cggttcgccg atcagcgccg caatggcggc tatgctcact
     3181 attatggagg taaacggtgt cagttttatt caagtgatgt cggtatgtct tcccacctct
     3241 tttgctgccg cgatggtcgc cgcttttatt gcttcccgac agggatgtga gttgcaggat
     3301 gacgaggtct accttgagcg cctgcaaaaa ggactggtgc aaaaatacga gaataatagc
     3361 agcatcaagc caggtgcgac gctctccgtc ggattgttcc tgctggcgac ggtcgctatt
     3421 gttattcttg ctgccttccc ccaactgcgt ccgggttttg atatcggtaa accgatggaa
     3481 acacgcgata tcattattat ctgtatgctc tctgccgcct gcctgatggt ggtgctgtgt
     3541 aagacggcga ccgatgccat tattctcacc tccacattcc gtgcggggat gagttcgctg
     3601 gcggtaattc tcggcattgt aaccttaggc accactttta ttgatgcgca tttaactgag
     3661 attaaagata ttgctggtga tattttgcag gcgtatccga tgttgttggc gctggttctt
     3721 tttggcactt gcgcgctgct ctactcgcaa ggtgcgacca cgccgttaat tatcccgctt
     3781 gccgtcgcgc ttgacgtgcc cacctgggca attctggcgt cctatgtggc cgtcaccgga
     3841 gtatttgtac tgccaacgta tccaacatca ctggctgcca tggaatttga tactaccggc
     3901 acaactcgtg taggcaaata tgtgctgaat catccattta tgctgcctgg gttgggggga
     3961 attattgctg gagtggcatt aggctttgtg attgcgccaa tgattattta gcactatcaa
     4021 acatcagtat cgatggttta tacacaataa agacagatat ggcggaatat agtctgttgt
     4081 aagcgccaac aaagaatagg ggaattccgc catgtctgaa catcttcaca atatggtatt
     4141 aagtgttgtc gtttaatgat ttatttcttg gttatgttgt gtattagctg catctatcgc
     4201 tttttgatac ctgaggtgta ctggcaatag cggacactac catttgttct ttttttaagc
     4261 agtcatctga tgatattttt ccctgaaggc tgccg
//
LOCUS       DADWTH010000211         4262 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.217, whole genome shotgun sequence.
ACCESSION   DADWTH010000211 DADWTH010000000
VERSION     DADWTH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.217"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..2057
                     /locus_tag="JNY02_004102"
     CDS             <1..2057
                     /locus_tag="JNY02_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB9435383.1"
                     /translation="SMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQ
                     TRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPRLEGAWAPEH
                     SVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTH
                     LNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWENNPDALALV
                     DNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRIRFT
                     KSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAIIAHGAQ
                     GASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKG
                     TAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKY
                     PQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNG
                     ESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAG
                     NGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQER
                     DRAAITEREASLPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTPG
                     GD"
     gene            2077..2823
                     /gene="traX"
                     /locus_tag="JNY02_004103"
     CDS             2077..2823
                     /gene="traX"
                     /locus_tag="JNY02_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBB9435384.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            2878..3438
                     /locus_tag="JNY02_004104"
     CDS             2878..3438
                     /locus_tag="JNY02_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBB9435385.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            3569..3781
                     /locus_tag="JNY02_004105"
     CDS             3569..3781
                     /locus_tag="JNY02_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBB9435386.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            4025..>4262
                     /locus_tag="JNY02_004106"
     CDS             4025..>4262
                     /locus_tag="JNY02_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBB9435387.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT          993 a         1042 c         1390 g          837 t
ORIGIN      
        1 gctctatggt gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg
       61 gtcgggctgt ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt
      121 tccgtctcca gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc
      181 agacgccgga actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc
      241 tgtccgggct tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac
      301 cggagcactc cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc
      361 agaaggcgat gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca
      421 ttgtccgcga ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc
      481 acctgaatga ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg
      541 gagaactggg gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg
      601 gggagctgcg tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata
      661 atgtgtatca ccggattgcc ggtatcagca aggatgacgg gctgataacc ctgcaggatg
      721 cggaaggtaa cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt
      781 acaccccgga caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc
      841 gggagcgcgg ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg
      901 tcacactttc tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc
      961 agcatattga cctggcctat gccatcatcg cccacggtgc acagggggcg agtgaaacct
     1021 ttgccatcgc gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag
     1081 cctacgtggc cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg
     1141 gctggacgga tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc
     1201 cgaaaccgga ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc
     1261 gggacgtggc ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc
     1321 ctgcacggtt tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt
     1381 ttgaccgtaa cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa
     1441 acgggctgcg gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg
     1501 tggccctgca gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg
     1561 tccggattgc ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc
     1621 gtccgtggaa tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca
     1681 gcgtccagcc gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc
     1741 aggctgaaga ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa
     1801 tggcagagaa caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg
     1861 ccggtcagga gcgtgaccgg gctgccataa ctgaacggga agcctcgctg ccggagagtg
     1921 tactgcgtga accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc
     1981 tgcaggagcg tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa
     2041 ccccgggcgg agactgatac aggaagacaa acactgatga caacggataa cacgaacacg
     2101 acacgtaacg attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg
     2161 tcacccggac agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac
     2221 atcaatctga tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt
     2281 ccgctttttg ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg
     2341 gccattaacc ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc
     2401 tttccttggt atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg
     2461 tggtgtgaaa cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg
     2521 gggcctttgt ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac
     2581 cggctgtacc gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct
     2641 gttattccgg cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg
     2701 acggtgctga ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc
     2761 ggagactttt tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg
     2821 tgacgggtgt ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg
     2881 atagagcaga agcgaccggt actgacactg aagcggaaga cagagggaac agcgcctgtc
     2941 cgcagccgga aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag
     3001 aagctggccg agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga
     3061 caggcgctgt ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag
     3121 ccctggtggc cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg
     3181 gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc
     3241 agggcgctga aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc
     3301 tgccggtatg acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat
     3361 gcgggagcgc gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc
     3421 gtgcttgatg agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt
     3481 aaaaggaaat aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata
     3541 atactgacaa ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg
     3601 ccaggtctgg cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg
     3661 ctggatggaa gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg
     3721 gcagcagttt gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta
     3781 acgaaaaaag cagccttcat tactctagga gtcagaaggc tgaaaggagt ttatgagatt
     3841 tgcttcttaa tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt
     3901 tccgcattaa gtcgattttt tatattttgt gattaatttc acaaaataag gtgttattca
     3961 atgtgtgctg cactattcag ggggtctgaa atacaggtgg catttatctg atatggagaa
     4021 taacatgagg aaatatattc cactggtatt atttatcttt tcatggccgg tattgtctgc
     4081 agatattcat ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc
     4141 actgaaggcg gtgcggatca ggctggtaaa tattgatgca ccggaaaaga aacaggatta
     4201 tggccggtgg tcaacagaca tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac
     4261 at
//
LOCUS       DADWTH010000212         4233 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.218, whole genome shotgun sequence.
ACCESSION   DADWTH010000212 DADWTH010000000
VERSION     DADWTH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.218"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..164
                     /locus_tag="JNY02_004107"
     CDS             <1..164
                     /locus_tag="JNY02_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435388.1"
                     /translation="ALTAIVGFAGMIITWIVKSFDEDVDYYVPVAEIEKLENQHFDEI
                     TKAGLKNGN"
     gene            154..768
                     /locus_tag="JNY02_004108"
     CDS             154..768
                     /locus_tag="JNY02_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBB9435389.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            768..1097
                     /locus_tag="JNY02_004109"
     CDS             768..1097
                     /locus_tag="JNY02_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBB9435390.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            1109..1999
                     /gene="cyoE"
                     /locus_tag="JNY02_004110"
     CDS             1109..1999
                     /gene="cyoE"
                     /locus_tag="JNY02_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBB9435391.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FSANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            2184..2495
                     /locus_tag="JNY02_004111"
     CDS             2184..2495
                     /locus_tag="JNY02_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBB9435392.1"
                     /translation="MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            2479..3021
                     /locus_tag="JNY02_004112"
     CDS             2479..3021
                     /locus_tag="JNY02_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB9435393.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPATINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(3077..4012)
                     /locus_tag="JNY02_004113"
     CDS             complement(3077..4012)
                     /locus_tag="JNY02_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBB9435394.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT         1030 a         1004 c         1019 g         1180 t
ORIGIN      
        1 gggcgcttac ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
       61 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
      121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
      181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt
      241 tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac ctatgccgtt
      301 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
      361 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
      421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
      481 gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt
      541 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
      601 gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
      661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
      721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
      781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
      841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg
      901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
      961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     1021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     1081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     1141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     1201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     1261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     1321 gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     1381 cttgctgggt attgctggct ttatgctgct gtggtttagc gcgaatccgc tggcctgctg
     1441 gctgggggtg atgggctttg tggtttatgt cggcgtctat agcctgtaca tgaaacgcca
     1501 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg tgattggcta
     1561 ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     1621 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt accaggcggc
     1681 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     1741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     1801 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ctctgcgcgg
     1861 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     1921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact cgcatacgct
     1981 gctggctgct gtgtggtaac aaaacctctc tattaaaaag gtgctacggc acctttttta
     2041 ttgtctgaga tcagagatac ggctaataac cgtatctctt gacagagtga gcagatgcac
     2101 ataaattaat catacggttg atagccgtat tatgtatgag atcgactccc tacgatttca
     2161 ttcagttaat caaagaagtc ataatgcttg gaaaaacagc cgctctatac gatgtcgata
     2221 aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa aacaaacttc
     2281 gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc gctgctatgg
     2341 aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat caaagctggg
     2401 aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc aaggcgttaa
     2461 tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc catagtcctc
     2521 tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga ttgcggtaac
     2581 cctgttatcg ataaatttgt acgcgcatcg ctaaagaaaa gtgtgcgtaa tagcgactgt
     2641 gcggctaaag cacttattga cagacaaagt ggtgaactga ttggcatctg tacttttacg
     2701 gcatattcgc tggaaagaca acgcgtttct ggcgtccttc agggttcaca accttcagaa
     2761 attggtgttg tcagattagt catgttgggg ggagcgcgga agtatcaaaa gcggggcttt
     2821 ggtcaggacc tactatgtga tttttttgaa catgtaaaaa aaattcacca ggcattacca
     2881 attaaagggg tttatcttga tgctgaccct gccaccatta acttttatgc tcgtctcggc
     2941 tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt tttggcgatt
     3001 cagcatattc tcgcggctta gattattatc gccacccgcc agggcaagtt ttgccttagc
     3061 cgggggctga gcagcgttac cacttttgtt caattgcccc cgctttcagc aactccacat
     3121 ccttgccgtt aatagttgcc gttgcggcaa cgattgtggt ccttacagtt gcacaagatt
     3181 ttcgctcaaa ttccggttta taaagaataa gagacgaatt actggtaaat gaaccaaata
     3241 ggtaatcagt aaagctaata ttcagccggt ttaaaccgaa aggcgcgaag ccaaccgttt
     3301 ggttaatctc cttaccggat gggcgacgaa tgctgaatgt cacgcgaaga ttggccactt
     3361 cgcctaattt attggtggtg gcgaagaggc tatccatgca ggaaaaacct ttatctaaaa
     3421 aattatcccg catcccagaa cgggtaaccg tgatcacggg taacgtatcc tcaaccgttt
     3481 ttctgtcaat catgtctttt cgacaagtag ccagaaagtc ttccgggcca ccataaatgt
     3541 ggacaccttt atcaaggtat tccttcgctt tcttacaatc tacctcaaca tactgaccat
     3601 tggcgtacat catgcccaac ctggaccatg ctaaattcat accttgctgc gcggcctgat
     3661 taaatagtgc atacgctttg gcataatcca cccgcacctg ctcaccgtcg tagtagatac
     3721 tccccagcgc taacggagct tctctgtcac cttgtttagc ggctaactcg aaccatttaa
     3781 tcgcttcctg aacatcaggt ttggtatcat agcgaggctg ataatattcc cagcctaaca
     3841 ggctctgcgc gtcgatatct cccgattcag ccattttatg cagtgtattt ataacatcgg
     3901 gagcagattt accaccgtat atgctgtcat acaattcatg cgctttctta cttgccacca
     3961 cgcctgatga aactatcagc attaaagaaa ataatattct attaggcaac ataaaatatc
     4021 tcttatttat cttgtacctg aatatgctct tcttttaata aatcacgttg ttttccattc
     4081 agtatcgctg tcgcagattt aatagaataa gtcaaatcat gaaaatcgca taaattacga
     4141 ttatacattg ggatgtctat atagcttttt cttatataac tggaattatt atacatatcc
     4201 acaccgagcg tattcaaact aaaaggaagg aca
//
LOCUS       DADWTH010000213         4129 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.219, whole genome shotgun sequence.
ACCESSION   DADWTH010000213 DADWTH010000000
VERSION     DADWTH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.219"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..158
                     /locus_tag="JNY02_004114"
     CDS             <1..158
                     /locus_tag="JNY02_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435395.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="JNY02_004115"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="JNY02_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBB9435396.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="JNY02_004116"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="JNY02_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBB9435397.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1293
                     /locus_tag="JNY02_004117"
     CDS             874..1293
                     /locus_tag="JNY02_004117"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBB9435398.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1280..4099
                     /gene="traI"
                     /locus_tag="JNY02_004118"
                     /pseudo
     CDS             1280..4099
                     /gene="traI"
                     /locus_tag="JNY02_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
BASE COUNT          930 a         1060 c         1293 g          846 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac
     1261 ggacggacag tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga
     1321 caccagtcgc gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca
     1381 gtataacggc gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga
     1441 gaatgtgtac gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca
     1501 ggttgaggcg ctgggctatg aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc
     1561 gggtgtaccg gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga
     1621 agacgcctcg ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca
     1681 cgtcgatcct gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga
     1741 catccgggca tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg
     1801 acccgcttca caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt
     1861 aagtgaacgc aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact
     1921 gccgccggaa aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagccg
     1981 tgagcagctt atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct
     2041 cgatgagctg tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt
     2101 acatccggag aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc
     2161 acaggaccgt ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga
     2221 gcgggtggct gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc
     2281 tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat
     2341 aaccggacgt cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt
     2401 cgaccagggc gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg
     2461 tcataacgta caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact
     2521 gatggccatg aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc
     2581 ggccaccatc atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt
     2641 tgcggccagc gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca
     2701 ggccatactg acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc
     2761 ggaggtaacc atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg
     2821 ggatatgtac cgcccgggaa tggtgatgga gctgtggaac ccggagacac gcagtcatga
     2881 ccgctatgtg acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca
     2941 gggcgaaacg caagtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc
     3001 ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact
     3061 ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt
     3121 tgttgtgccg ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc
     3181 actgaagctg gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac
     3241 ggtttttgcc tccgtcacac agatggcaat ggacaatgcc accctgaatg gtctggcccg
     3301 cagtggtcgt gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact
     3361 tgcccgccat ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac
     3421 attgctggaa accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc
     3481 cattcatctg gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct
     3541 gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga
     3601 aatcaatgcg cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg
     3661 cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat
     3721 tctcgaaggt aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga
     3781 gaaactgaca tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt
     3841 cacggtggtg cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc
     3901 agccgtgaac atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca
     3961 ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct
     4021 gcatgacacg cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttccttct
     4081 cgatgagagc tctggtaatg ctgccaactt actgatttag tgtatgatg
//
LOCUS       DADWTH010000214         4060 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.220, whole genome shotgun sequence.
ACCESSION   DADWTH010000214 DADWTH010000000
VERSION     DADWTH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.220"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JNY02_004119"
     CDS             complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JNY02_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBB9435399.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JNY02_004120"
     CDS             complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JNY02_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBB9435400.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     THKALEDRS"
     gene            complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JNY02_004121"
     CDS             complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JNY02_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBB9435401.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GNVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2849..3763)
                     /gene="sitA"
                     /locus_tag="JNY02_004122"
     CDS             complement(2849..3763)
                     /gene="sitA"
                     /locus_tag="JNY02_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBB9435402.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPMYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1102 a          995 c          988 g          975 t
ORIGIN      
        1 tgcaggcgca ggtcgatcgc aacgatgaga tttatcgccg tcagcgggag ctgaaggaag
       61 agctgaatag tctgaaggat ttgaattcgg ttaggaattt tatcgtggaa taacagaagc
      121 tgcggcacgt cgtatgcagg aacgtgccgc ggttggctgg taaactttcg atagtgcgag
      181 tattgaatga tttctaggcg ttatcgattt tacgtatttt ttgcatgata gaattcgtac
      241 ctcctcccaa cgaccttcca tggctttccg gacttctgta ggctggcgga cgatgctgtc
      301 ctcggatgtt gtgctaattg ggtgataaca gatcctgtgt atgggcgttt ccctttgtgc
      361 gtgcgttgat gctggtaacg gtaaacgtca tgatgaacac gaccgcgaat aagacgacaa
      421 tcgtcggagc tggtgcgctg tcgataaaaa aggacagcca gacaccagtc attgacacta
      481 ttaccgatat gccagtcgcc agtaggagcg caatatgaaa gcgttgtgtc aggagtactg
      541 caatggcgcc aggcgcaatg agcagagaaa tagagagaat gatacctact gctttcagcg
      601 tcgccacaat ggtcagggaa accatgcaca gcagcccata atgcagccat ctcgtatgca
      661 aaccgcttac ctgcgcctgt tgataatcaa agctgaacag caaaaaatcc cgccatttta
      721 cgctaataac aagtgtaacc agcccggcaa taatcaccgt ctggattata tcgccgatgg
      781 ttatcccgag catgtcgccg aaaagaatat ggtcaagatg tacgtctggt ttaattgcaa
      841 tatacaagat cagacctgcg gcaaacatgc cagaaaaaac aatccccata actgtatctt
      901 gcttgattcg gctgttgtct ttcaggtatc ccgtcgccac cgcgcagaat actccggcga
      961 cgaatgcccc tgtagccaga ggtaacccca gaatccaggt aagcacgatg ccaggaaaaa
     1021 ccgcatgact catcgcgtca cccatcagtg cccaaccttt caggaccaga aagaccgaca
     1081 gcagcgcaca gggtatggaa actaccagtg aaatcagcag tgcgtgactc ataaaggtga
     1141 actgcagtgg ctccagtaat aatgccatca tgagcgatcc tccagcgctt tatgcgtacg
     1201 ccgacgattt gccaaaagtc catgggtggg cgcaaataca aatgctaaaa gaaacagcag
     1261 tgtctgcgcc accacgatga ttccacctgt cgcgccgtcc agaaagtaac tgacccacgc
     1321 gccgaggaaa ctggttacac tgccaatagt tacagcaatt atgagcaggc ggggaaaacg
     1381 atcggttaac aaccatgctg ttgcaccggg agtcaccacc agacagatca ccagaaaggc
     1441 accaaccgtt tgcagcgccg caacggtcga aaccgacagc agggtgaaga agaggatttt
     1501 taatctgccc ggatgtaatc caatagcacg ggcgtgattc tcgtcgaaaa aggtcaccat
     1561 aagatctttc catttgaaaa aaagcacgat gatcgagaga atgccaatga tagtgagttg
     1621 caggatatcc gccggatcga ttgccagaat attgcccagg acgatggtct gaatgttcac
     1681 ggatgtcggg ttcaatgaca ccataaacag ccccaggcca aaaaaggacg agaagatcag
     1741 gccgatgatg gcatcttctt ttaagcgggt gcgttggtta agaaacagca tgctgcccgc
     1801 tgccagtccg ccggaaaaga aggctcccag agaaaacgga agccccagca tatatgcccc
     1861 cgcaacaccc gggacaatag agtgcgacag cgcatcgcca atcagcgacc agccttttag
     1921 catcagatag catgacagga aagcgcagag accgccgacc attgccgaga cccacatcgc
     1981 attgagcata tattcatagc tgaagggttc cagtagcacg ttcatcttgc ttccctctgc
     2041 accgctgacg gtcgatgtgc cacaaaaggg cgttcatcgt cagtaataat gctttcttcc
     2101 gagccgttaa gtgtgacatg gcgcagtacg ccgctaaaag ccagctccag gttctcggct
     2161 gtaaaagtgg tgtctgtcgg cccgcttgcc aggactgtgc ctttgaccat gaccgtataa
     2221 tcgcaaaatg tcgtgactga gccgagattg tgcgttgaga caagcatcgt tttgccttcc
     2281 gcgcgtaact ccctcaacag gctgatgatt ttcgcttcgg ttttcacatc aacgccggta
     2341 aaaggttcat ccagcaggat cacattaccc tgctgtgcaa tcgctctcgc cagaaagacg
     2401 cgtttctttt gtccaccgga aagctcgccg atttgacgat ggcgaaaatc caccatatcg
     2461 acacgttcca gcgcatcggt gacaatctga cgatccctct ttttggctat acgcaacata
     2521 cccatatgcc catagcggcc catcatcacc acatcttcca ccagtacagg aaatgaccag
     2581 tcaacctctt ctgactgagg aacgtaggca accaggtttt tctgtagcgc ctgtcgcgtg
     2641 ggaataccca gaacagatat ctttccgctg gtcagacgca caaatcccat aatcgcttta
     2701 aacaacgtcg attttccgga accgtttacc ccaaccagag cggcaataga tccacttggt
     2761 actgtgaagg atgcatcgcg cagtgctgtg tgcccgttac gccaggtgac agtaacatca
     2821 ttaacgacaa tgcctgcaga ttgcatcatt atttctccct tttcccggct ttaattccct
     2881 gtaccagcgt actggtagta accttaagaa ggtcgatata cataggtacc gggccgtttt
     2941 ctgtgctcag ggaatcgaca tagagcacac cgccgtagtg tgcgccggtt tcacgcgcaa
     3001 cctgacgcgc tggtttatcg gaaatcgtac tctcgctaaa gactgccggg atatgatttt
     3061 tcttaactat atcaacaacc ttacgtacct gctgcggtgt tccttgttga tcggcattaa
     3121 tcggccacag ataaagctct tttagcccca aatcgcgtgc gagataagaa aaagcccctt
     3181 cactggtgac catccatcgc tgattctcag ggagttccgt aatctgctta cgcagggggg
     3241 caagggtttg ggtaatcttg gctttataag tatcggcatt acgttggtag gtttgtgcat
     3301 ttgccggatc gtattttatc aacgcatcac gaatattatc gacgtaaatc agagcattat
     3361 ctggcgacat ccatgcatgg ggattaggtt tgccctcata gggtccttcg gtgatcccta
     3421 ctggcgtcac acccgaagag acaattactt ctggaacccc attgagatgc tggtaaaagc
     3481 gttggaacca caattccaga ttcataccat tggcgagaat cagttgtgcc ccctgcgcac
     3541 gtttaatatc gccaggggta ggctgatact catgaatttc tgcaccaggc ttggttatgg
     3601 atgagacttc tgcagcatct ccagccacgt ttttggccat atccgcgatg atggtaaatg
     3661 ttgtaatgac cttgaatttt tcagttgcac ttgcctgaaa tgcgatcgag caggtgaggg
     3721 cgaggcaccc caagagcatg gttacttttt ttattgagtg cataatttag tacctatagt
     3781 gagcaaaatt agaacaaaca tagcaacggc tatgttttat agcataacct atacaaaatg
     3841 aaaataatta tcattaacag ctttgaggcc taaaacaggg attaggttgt ttcctgctgg
     3901 cgacatgata ttgaggaaaa aagtagagca gctattaggc tttgaaatga ctttatgagg
     3961 aaaattgttg atggcaaaaa tggggcaaaa cgctgtataa ggggcaaaaa agagtgggtt
     4021 atcgtagctt attgttgtcg ctgataatgt ttaacatatt
//
LOCUS       DADWTH010000215         4011 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.221, whole genome shotgun sequence.
ACCESSION   DADWTH010000215 DADWTH010000000
VERSION     DADWTH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.221"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..68)
                     /locus_tag="JNY02_004123"
     CDS             complement(<1..68)
                     /locus_tag="JNY02_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HBB9435403.1"
                     /translation="MFSMILSGLICGALLGFVMQRGR"
     gene            complement(247..1606)
                     /gene="plaP"
                     /locus_tag="JNY02_004124"
                     /pseudo
     CDS             complement(247..1606)
                     /gene="plaP"
                     /locus_tag="JNY02_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
     gene            complement(1596..1676)
                     /gene="yoeI"
                     /locus_tag="JNY02_004125"
     CDS             complement(1596..1676)
                     /gene="yoeI"
                     /locus_tag="JNY02_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBB9435404.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(1874..2803)
                     /locus_tag="JNY02_004126"
     CDS             complement(1874..2803)
                     /locus_tag="JNY02_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9435405.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2849..3673)
                     /locus_tag="JNY02_004127"
     CDS             complement(2849..3673)
                     /locus_tag="JNY02_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB9435406.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3756..4010)
                     /gene="yoeB"
                     /locus_tag="JNY02_004128"
     CDS             complement(3756..4010)
                     /gene="yoeB"
                     /locus_tag="JNY02_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBB9435407.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1091 a         1040 c          935 g          945 t
ORIGIN      
        1 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
       61 gaaaacataa attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga
      121 atgcagacca ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga
      181 taaaaaagaa taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa
      241 agatgattat gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca
      301 agccaggtaa atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc
      361 caggttaacc cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg
      421 gaagtgatct ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga
      481 caggttaaca aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac
      541 caggtcgaag ttgattgcca gcagggcaat cgagccaacc aggatgatgt tcatcgccgg
      601 agtacgccat ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc
      661 cattacgtac atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt
      721 aatggtggag aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat
      781 ttcaggctgt gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag
      841 gaagtaagtt gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg
      901 cgggatcacg cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc
      961 ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc
     1021 agaccagaac ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata
     1081 aacaaccatg cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt
     1141 gaagttcgct acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagccg
     1201 ccaccacaaa catccacgat ggaatggaag gcaccagagc ttcaaaatag attttcgcca
     1261 gcagaatgtt gatcatcggc gcgaacagat agtcgagcag agaagaccaa cccaccataa
     1321 agccgacagt cgggctaatg gatttctggg cgtaagtgta tgcagagcca gcagaaggat
     1381 agcggcgaac cagcttcccg tagctcagag ccgtaaacag gatcgcaatc aatgcgaacg
     1441 cataggctgt cggcacatga ccatccgtga ggcctgaaac gataccaaat gtatcaaaca
     1501 gcgtcatcgg ctgcatatag gctagaccca tcattacaac cggaactaac gtaagcgttt
     1561 tacgcaattc cacgcgagag gtgtttggag taacgttatg cgacatggtt attctccttt
     1621 acggtgataa ccgtcgcgta agcaaaaaat ttgccccatt tttttggatt cctcagcgac
     1681 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
     1741 tgattggttt gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt agtcattttt
     1801 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
     1861 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
     1921 tcgcactatt tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt
     1981 cattccagcc aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta
     2041 acgcgccact atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc
     2101 cgctaatttg cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
     2161 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt gtcccccacc acaatcgccc
     2221 gatatcgttt aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag
     2281 gcgcgaccgc aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag
     2341 atggtggctc atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc
     2401 aggaaccacc gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
     2461 aagcttcaat gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
     2521 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat
     2581 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
     2641 gtccgctacg atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac
     2701 tcagagcgga cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt
     2761 ctatggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt
     2821 gcatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
     2881 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
     2941 cgctgtccag actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg
     3001 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
     3061 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
     3121 caccatgttc accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg
     3181 gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
     3241 agtcttcgag ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt
     3301 ctttaaccgt gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg
     3361 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
     3421 cgccggggcc gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg
     3481 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
     3541 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
     3601 agcctcgcgc tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg
     3661 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
     3721 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
     3781 atgagcagtg aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
     3841 gaccagaaac ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt
     3901 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
     3961 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac t
//
LOCUS       DADWTH010000216         3977 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.222, whole genome shotgun sequence.
ACCESSION   DADWTH010000216 DADWTH010000000
VERSION     DADWTH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.222"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="JNY02_004129"
     CDS             complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="JNY02_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBB9435408.1"
                     /translation="MRAPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(331..1680)
                     /gene="lysC"
                     /locus_tag="JNY02_004130"
     CDS             complement(331..1680)
                     /gene="lysC"
                     /locus_tag="JNY02_004130"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBB9435409.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2205..3854
                     /gene="pgi"
                     /locus_tag="JNY02_004131"
     CDS             2205..3854
                     /gene="pgi"
                     /locus_tag="JNY02_004131"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBB9435410.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1008 a         1050 c         1004 g          915 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg tggtgccctc agtgagccag agagttcagt gtacgggaat
      121 tcgtttgcga gcagaactga taatattcat tttgccttct cttaactatt tatctctttc
      181 gataattaat tagagatgca gataaaaaaa ttaaaggcaa ttattctccg ataaagacgt
      241 tggttaacag agtacaggct cgtttattga tgagcatagt gacaagaaaa tcaatacggc
      301 ccgaaatata gcttccaggc catacagtat ttactcaaac aaattactat gcagtttttg
      361 caccacctgc tcggcatctt cgccgggcac caggaagcac aggttatggc tggatgcgcc
      421 gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg aatacctctt tgccaacgcc
      481 gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc gccagacctt cttccacctc
      541 cacccggcac agtgcggaaa gctccatcag cagagattgc gtcagcaacg tatcgccagt
      601 ggaggttgaa ccggtggtat caagggttaa tgccacgctc acttctgacg tggtgattaa
      661 gtctaccgaa atattatgcc gcgcgaggat gccgaaaact tccgcgagga agccgcgaga
      721 atgcagcata ttcaggctgt gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc
      781 gcggaacagc ggcggatttt cagttttatt gcacaccagc gtaccacctg cgcgtgggtc
      841 tttgctggag ccgacaaaga ccgggatatc gctgcgtact gcgggtagca acgttgccgg
      901 atgcagtact tttgcaccaa aagttgccat ctctgccgct tctgcaaacg cgatttcatc
      961 aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt
     1021 ccagatatca acacgagatg cgtgtaaagc ctccgccagc aaggctgccg tataatcgct
     1081 gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg ctaccgataa atccctgggt
     1141 gatcactaag ccttcattga ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc
     1201 ggctacatct ggctctgcac gaccaaatcg gtcgttggta cgcatcactt tacgtacatc
     1261 aaaccactgt gcctgaacat cgcgttcgcg caggatttcg acaaacagca gggtcgacat
     1321 cagctcgccg tggctgacca gctcatctgt cagcgccgga gacgttgcca gcgccgccgc
     1381 ttctgccaga acagtaatgt tctccagcag acgttcaatc tcttcacgga taacgttcgg
     1441 gtaacgcaga cgttccagaa tggcaaactg gatgttgcga atagcgtcga gtttttcgaa
     1501 tcgctcgcca ggttccagcc cttccgctaa agcgaccagc agattagtga taccagcaga
     1561 agccgagagg actactaaac gcacgttggc atcagaaagc acaatatcag cgctgcggtt
     1621 catggcgtca aaatcagcta cgctggtacc gccaaatttg gagacaacaa tttcagacat
     1681 aactacctcg tgtcagggga tccattttca gccttggcac aagggaagag cggaagacgg
     1741 gtgggcgcag agcgatactt cgctactatt ttcacccaga agtgctccac cacttgcgaa
     1801 acgcccgact gcgaacgctt ctggtgacaa cccaggggat tcagcccctg tagccgatga
     1861 tgaacgtggc cagccgttca atcacctcgg cgatgcaccc cctcaggtgt tatcacagga
     1921 ctggctcctc caacaccgtt acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg
     1981 tagtacattt ataaataaag ggtgagcggg gcggttgtca acgacggggt catgcggatt
     2041 tttcatccac tcctggcggt cagtagttca gctaataaat gcttcactgc gctaagggtt
     2101 tacactcaac attacactaa cggcactaaa accatcacat ttttctgtga ctggcgctac
     2161 aatcttccaa agtcacaatt ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc
     2221 caacgcagac cgctgcctgg caggcactac agaaacactt cgatgaaatg aaagacgtta
     2281 cgatcgccga tctttttgct aaagacggcg atcgtttttc taagttctcc gcaaccttcg
     2341 acgatcagat gctggtggat tactccaaaa accgcatcac tgaagagacg ctggcgaaat
     2401 tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg
     2461 agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca
     2521 ataccccgat tctggttgat ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga
     2581 agatgaaaac cttctcggaa gcgattatct ccggtgagtg gaagggttat accggcaaag
     2641 caatcactga cgtagtgaac atcgggatcg gcggttctga cctcggcccg tacatggtga
     2701 ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg
     2761 ggactcacat cgcggaagtg ctgaaaaaag taaacccgga aaccacgctg ttcttggtag
     2821 catctaaaac cttcaccact caggaaacca tgaccaacgc ccatagcgcg cgtgactggt
     2881 tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca
     2941 atgccaaagc cgttggcgag tttggtattg atactgccaa catgttcgag ttctgggact
     3001 gggtcggcgg ccgttactct ttgtggtcag cgattggcct gtcgattgtt ctctccatcg
     3061 gctttgataa cttcgttgaa ctgctttctg gcgcacacgc aatggacaag catttctcca
     3121 ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct gattggcatc tggtacaaca
     3181 atttctttgg tgcggaaact gaagcgattc tgccgtatga ccagtatatg caccgtttcg
     3241 cggcgtactt ccagcagggc aatatggagt ccaacggtaa gtatgttgac cgtaacggta
     3301 acgttgtgga ttaccagact ggcccgatta tctggggtga accaggcact aacggtcagc
     3361 acgcgttcta ccagctgatc caccagggaa ccaaaatggt accgtgcgat ttcatcgccc
     3421 cggctatcac ccataacccg ctctctgatc accaccagaa actgctgtct aacttcttcg
     3481 cccagaccga agcgctggcg tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg
     3541 atcagggtaa agatccggca acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta
     3601 accgcccgac caactccatc ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga
     3661 ttgcgctgta tgagcacaaa atctttactc agggcgtgat cctgaacatc ttcaccttcg
     3721 accagtgggg cgtggagctg ggtaaacaac tggcaaaccg tattctgcca gagctgaaag
     3781 atgataaaga aatcagcagc cacgatagct cgaccaatgg tctgattaac cgctataaag
     3841 cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat gtaggccgga taaggcgttt
     3901 acgccgcatc cggcaaccgg agcctgatgc caatatcgac attaatgcat agtatgagga
     3961 gttaaataac cttaaaa
//
LOCUS       DADWTH010000217         3856 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.223, whole genome shotgun sequence.
ACCESSION   DADWTH010000217 DADWTH010000000
VERSION     DADWTH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.223"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            229..336
                     /locus_tag="JNY02_004132"
     CDS             229..336
                     /locus_tag="JNY02_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBB9435411.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            712..819
                     /locus_tag="JNY02_004133"
     CDS             712..819
                     /locus_tag="JNY02_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBB9435412.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="JNY02_004134"
     CDS             complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="JNY02_004134"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBB9435413.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="JNY02_004135"
     CDS             complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="JNY02_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBB9435414.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2770..>3856)
                     /gene="bcsE"
                     /locus_tag="JNY02_004136"
     CDS             complement(2770..>3856)
                     /gene="bcsE"
                     /locus_tag="JNY02_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBB9435415.1"
                     /translation="EEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQN
                     GIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAA
                     TVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVI
                     PWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMM
                     NNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRIND
                     LDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVI
                     NAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT          967 a          980 c         1046 g          863 t
ORIGIN      
        1 gtgacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg
       61 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac
      181 aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacgattgc acgcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt
      541 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
      601 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggctaat cttgaaccca
      661 aacaactcaa gaatagccgc gaaacgttgg cattacaaca caggcggcta tatgacgctc
      721 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
      781 gccagtatga tcgtgaactg gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc
      841 tgccgtaatg gcaatttgcg cccggatcag gccgcagagg ttaaactctg cggccttttt
      901 cgtgcttact gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatct
      961 tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca
     1021 ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga
     1081 accaccagat cggagatagc caggaagctg ctcggttggt caatgacaat cggtgccccc
     1141 tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg
     1201 ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt
     1261 tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg
     1321 tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc
     1381 ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt
     1441 ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc
     1501 gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa ttcagtctgc
     1561 atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc
     1621 atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa acagatagca gtcgttattt
     1681 gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc
     1741 gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaactcaat atcgaaatgc
     1801 gaccacagtg gatgcgacat caacccggcg gcttctatat ccgaccagga aagcgaacag
     1861 atgttaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc
     1921 gatttacgtt tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gttcgccgtt
     1981 gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca
     2041 acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg tccggctggc
     2101 cacaaggaga aacttggtcc cgccagggta agtacgttca gccatagcag tatggcaacc
     2161 acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccactaa taaaacaaaa
     2221 atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta
     2281 ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccaag caaccaggtg
     2341 tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg
     2401 ctgtaacgcg gaatgggcat cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg
     2461 aagttaagat atcccgccca caacaggcca aacttaacca gaaaatagaa gttccagccg
     2521 gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga
     2581 gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat
     2641 aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg
     2701 ccagatagcc cagcgggaaa aatatcagtg cgcaaacgat aataatttca atgatatcgc
     2761 tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcatgg gttctggtat
     2821 tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa
     2881 aggcagcggc attccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact
     2941 gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg
     3001 gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac
     3061 cagccgatta ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag
     3121 ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg
     3181 ggcaggcaag agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt
     3241 ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg
     3301 tacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac aacgggagag
     3361 tggcgcattc cacggaataa ccatatttgc accgcaggcc aataacaaac gttcatcggt
     3421 ggcgcgcagg ctagcggtat tttcccgcac gaggattttc atcgcactac cgcgctggcg
     3481 acgcagggta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa
     3541 gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa
     3601 cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat
     3661 gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta atgtccagat
     3721 accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca
     3781 gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag
     3841 tgaacggtat tcctca
//
LOCUS       DADWTH010000218         3856 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.224, whole genome shotgun sequence.
ACCESSION   DADWTH010000218 DADWTH010000000
VERSION     DADWTH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.224"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1447)
                     /locus_tag="JNY02_004137"
     CDS             complement(<1..1447)
                     /locus_tag="JNY02_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBB9435416.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDRTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQ"
     gene            1831..2862
                     /locus_tag="JNY02_004138"
                     /pseudo
     CDS             1831..2862
                     /locus_tag="JNY02_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(2949..3425)
                     /locus_tag="JNY02_004139"
     CDS             complement(2949..3425)
                     /locus_tag="JNY02_004139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435417.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(3418..>3856)
                     /locus_tag="JNY02_004140"
     CDS             complement(3418..>3856)
                     /locus_tag="JNY02_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB9435418.1"
                     /translation="TISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEM
                     FAVAFKDTYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPG
                     ANFIYDVNSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT         1150 a          732 c          681 g         1293 t
ORIGIN      
        1 tctgtttagt ttttttgagc gtctgtccac atttgaagat atcttcaggt tttggtctga
       61 aagcagtagt gttattaaaa taattttcat atacaggtgt gggtgctata gcattgcttc
      121 taattagatg gagaagatga tcatccatgt gttgtttatg ttttttatta aatatatttg
      181 ctaattcctc aggatcaatt gggtctttca tctgattaaa tggaacctgc aaagcaccga
      241 gacaataaca ttgattttct tcatttctta ttattccgtg atgactggtt aaaactttag
      301 tttctgagtt ataattacaa acatcgagcg ttttaattaa gttatacgta tcaccggcag
      361 agagtttata attttctagt cgtgcagtat agttaccatt aaaattgatt tcatgatttc
      421 cagccagaac gatgacattt ttattaatac ggctatcttt attaccctcc atatttcgca
      481 tagaatttag caaagtaagg atatatttat caccaaaaat agtactaaat ctatcgcctg
      541 tatgatcccc aagggggata caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat
      601 gcaactcatt caatatcatg tctctcgtat ttaataaatt gtagagcaat ttattatttt
      661 tatgtatgta tgcagatgaa gccatgtctt cgtgttttaa taattcacat aataaactcc
      721 aacccttttc cctgatgcct atatgccctg aaacgagcaa tgaataaagt acagcgccta
      781 ctcttccatc tgtatcgcca aaataagccg tgttggcatt ttgtgggtta atcacaccac
      841 attgtttata aaagtctatt ttagaaaaca cagatttaac atagctatca aacttatcct
      901 taggaaacgt ctctttagag acatcccaac gccagtcagc tttaaaatta ccgctaagaa
      961 gagtaaagaa attttcaggt atgatagaaa gcccattaac tttataaatt atgtctgatt
     1021 tatctccgga gaaattaata aaataataac catccttaca ctcaatctta actgtaatgg
     1081 gagacgataa ttttatcaca tggttattgt ctggtataat tgtaaagata aacgtgttag
     1141 tagtattatc ggcaaaacta ttatattctt gaatatctat agatggctca ctattgtaaa
     1201 accatatttt ctccatctga agactatgtt catttaccct ccagtgacca tgttgcagga
     1261 aatagatgat atgcatgcca aaatcgttat ctatttgcga atcattatcc agtttatcaa
     1321 tttcaactat ctttgaacga tgtgttctat caaccttgct atggtgttga tgattattaa
     1381 ctttatctga atgcttatct ttatggtttg aagggatgta cttaaatata ttgtcaattg
     1441 ctttcataaa taacccgcga tatttttgac ctgttagtaa aaaataaatg atacatgtct
     1501 gaaagacata tttacgacga tataaaacaa taatacttat atgttcgctg attttaagca
     1561 ccgagactat ccggtctttt actaaataat ggaaaacaat ccatccctgg aagacaattg
     1621 taaaaaaaac aacagaataa gaattaatct taggataaat atttatttat catggccttt
     1681 tgaccgctaa aaaatggcac tcggcacagt aaaccccgct atagttatgc tatacatttg
     1741 tctttaatga tatctcgtcg ttatattaac aggctgaaat tatcattaat gcacatttca
     1801 atattagcag ggatacctga ggagtttaaa gtgaacatat atatcgggta gcttttcaaa
     1861 ttaatccctt tgattatagg cttaatttgc atcgcgttag gtggctttgt gctggaaagt
     1921 tcagggcaac gtcacgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt
     1981 cactaccgca tttattatca tttcgcagct cacgcgcggc gttaatacgt tttacaatac
     2041 attgttcccc attattggct atgcggggtc aattatcacc atgatatggg gttgggcact
     2101 gttagcaggt aatgatgtga tggcagacga gtttgttgcc ggccatgtta tttttggcgt
     2161 tggtatgatt gccgcatgtg tatcgacggt ggcagcgtcg tccggtcact ttctgctcat
     2221 tcccaaaaat gcagcgggga gcaagagcga cggaacaccg gtacaggctt attcttcatt
     2281 aatcggtaat tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc
     2341 tattacgctg ttacatagtg ccgacataac tccgcattat gtcgccttgg gttaaccgca
     2401 atctgtgcct gtctaattgg cctagtggcc acaatcgtcc atcaaacacg taatacgttc
     2461 tcatccaaag agcactggct gtggtgttat tgggttattt ttctcggctc agtcacggtg
     2521 ctgcagggga tatacgtctt agtcagttcc gatgcaagcg cccgactggc tcccggcatt
     2581 attctcattt gcctcggaat gatctgttac agcatattct caaaagtctg gctactggca
     2641 ctggtctgga gacgtacctg ttcgttagcc aacagaatac cgatgatacc ggtatttacc
     2701 tgtctgtttt gcttgttttt gtcatcattc ctcgcggaaa tcgcacaaac tgagctggac
     2761 tactttattc catctcgggt tctggttgga ttaagcgcag tatgttttac attgttctct
     2821 attgtttcaa tattagaggc cggttctgcg aaaaaaaatt aaaatttaca taatcccagc
     2881 gttattcata tcttaacgct ggttttttac gaacttagtg aaataatgta gaatataaaa
     2941 ttataatttt atattggagc acatgtgtta atgatcaacc ccgggatact aaaaatatag
     3001 acattattaa acttgcttag cctcgcattg cctttacctg ttaaagatgc atcgagaaaa
     3061 ttgagtctga agttctcatc ctttatctga ttttccagtc ccgaaacttt ggccttagca
     3121 ttacctgaga tccgtccgtt actatctatt ttgtaggtaa aaaaatacga atgggaaaat
     3181 ttcctcggtg tatcttcatc agttaagcca acaatcgtaa aactaccgct accatctttt
     3241 tttaaaatga ctgaaaggtt agcagagtag tataaagaat gatctgtttt agcatttctt
     3301 gttaacgtcg acgtacattc aagagacgtt gtgctaccat cacgagcctt ccagagagca
     3361 aaaaaacaca cctgtagcaa tgtgaaaaca agtattaata taaaatacat tttcttatta
     3421 gtcatataat agcacctctg aacaaattgc ataagtttcg tcattagagg attttataca
     3481 tcttgataaa taccgctgcc cttccccaag caaataatgc atactattta catcatatat
     3541 aaaatttgca cctggcacac agggtaaatg ctttttcgaa atttcagcgg cctctatttg
     3601 agatttagcg aggccaatct cgttaagatt ctctgaaaga tataaatcac aggtgtcaat
     3661 cttacctgat ttaaccaatg gataatccgt tatactgtag gtatctttaa aagccacggc
     3721 aaacatttca ataataacaa ttactaccac aagcgaaatc aaaatgcgaa aaagaggaat
     3781 actccttttg cgctctactt tttcaacaac atcttcttgt aaatgagaat catcataaga
     3841 ttgtttactt attgtt
//
LOCUS       DADWTH010000219         3671 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.225, whole genome shotgun sequence.
ACCESSION   DADWTH010000219 DADWTH010000000
VERSION     DADWTH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.225"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(623..2332)
                     /locus_tag="JNY02_004141"
     CDS             complement(623..2332)
                     /locus_tag="JNY02_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBB9435419.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="JNY02_004142"
     CDS             complement(2764..3483)
                     /locus_tag="JNY02_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HBB9435420.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1087 a          742 c          660 g         1182 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcactct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaatatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt ggctcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgcga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac gggcaggaat caggctgcat acgcagtgcc
     2701 aaatctaatc gtagttattc taaccagttt tcaagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg
     3601 ggaaggctta gaacgccata ttcagtttcc catagtggaa catcactccc agcttaaagc
     3661 gctcccggtt g
//
LOCUS       DADWTH010000220         3603 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.226, whole genome shotgun sequence.
ACCESSION   DADWTH010000220 DADWTH010000000
VERSION     DADWTH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.226"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..107
                     /locus_tag="JNY02_004143"
     CDS             <1..107
                     /locus_tag="JNY02_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB9435421.1"
                     /translation="ERESFNSAADHRLAELITGKLYDRIPKEIWKYVR"
     gene            146..736
                     /locus_tag="JNY02_004144"
     CDS             146..736
                     /locus_tag="JNY02_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBB9435422.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            976..1236
                     /gene="repA"
                     /locus_tag="JNY02_004145"
     CDS             976..1236
                     /gene="repA"
                     /locus_tag="JNY02_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBB9435423.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1460..1534
                     /gene="tap"
                     /locus_tag="JNY02_004146"
     CDS             1460..1534
                     /gene="tap"
                     /locus_tag="JNY02_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBB9435424.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1527..2384
                     /gene="repA"
                     /locus_tag="JNY02_004147"
     CDS             1527..2384
                     /gene="repA"
                     /locus_tag="JNY02_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBB9435425.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3084..3275
                     /locus_tag="JNY02_004148"
     CDS             3084..3275
                     /locus_tag="JNY02_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435426.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          955 a          908 c          901 g          839 t
ORIGIN      
        1 gtgaacgtga atcatttaat tcagcagcag accaccggct agcggaatta atcaccggaa
       61 agttatatga caggatacca aaagagatat ggaaatatgt aaggtaataa ttcagcaata
      121 attaattgcc aggagcagat aaataatgaa gttgattatt tttattctca ttgttctgat
      181 tattgcaacg ctacttatcc ggattatcct cagaagtgta aatcagcatt ctcctttgct
      241 gatgcaattg catgcggcag gtatacggac tggtgatgca gagcgaatac tgtccagcgg
      301 tgaatactgg caacgtcaga agacgctgct gacagaaagg gaagtcagtt ttatgaaagg
      361 actgttcaga attgtggata tgaagcggtg gtatctgtgt ccgcaggtac gggtcgcgga
      421 tatcgtccag ctgaacggga atatccggcc acgatcacgc cagtggtggc agttattcag
      481 gatggtgtct cagtggcatg ttgatgtggt tatcgttgag ctgcgttcgt tcagtattgt
      541 tgcagcagta gagctggatg atgccagcca tttacgaccg gaacgcagac gccgggatat
      601 tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc acgatgccag
      661 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc agcagtcacc
      721 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
      781 gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
      841 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgccacaa
      901 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttgag
      961 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt gagtcaaaag
     1021 cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc cgctgttgca
     1081 agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct gaaagatctc
     1141 atggttgagt actgcgagag agaggggata acacaggctc agttcgttga gaaaatcatc
     1201 aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt ggcgtagcat
     1261 gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg tggcagggaa
     1321 ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa tcttctttaa
     1381 ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa caatcaagct
     1441 atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt gctcgctcct
     1501 tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat taccgccagg
     1561 taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt ccgttctgcg
     1621 ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc attcatgtgg
     1681 cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt cgccgggcca
     1741 ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac cgcgtccagt
     1801 gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct gccggaaaac
     1861 tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga ctgattacct
     1921 accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc acgttcacat
     1981 ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg cgccgcagtc
     2041 gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg ggtatggatg
     2101 aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac cagacagagc
     2161 ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg cagcgtcagg
     2221 atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg cgcttcactg
     2281 ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc atgattctgt
     2341 cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga cctcctcaga
     2401 ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc acaaaaaatc
     2461 agcgctacat acaaaaaaca acctcatcat ccaccttctg gtgcatccgg tcccccctgt
     2521 ttttgataca aaacacgccc cacagacggg gaattttgct tatccacatt aaactgcaag
     2581 ggacttcatc gtaaggttac aaccgttcac gtcataaagc gccagccgtc aaccctacag
     2641 ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta attacattca
     2701 tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acagatatgc acctcccacc
     2761 gcaagcggca ggccccaacc gaagccggtt tagttacaac atgcaaacat aaacccctaa
     2821 aaaacccaag tccaacgcag aactgaaacc acaacgcccc tcctcataac tgaaaagcgc
     2881 cgccgccccg cccgaagggc gggaacagag tcgcttttaa ttatgaatgt tgtaactaca
     2941 tcatcatcgc tgtcagtctt ctgcctggac gtatcgagta cacgctcgta agcggccctc
     3001 acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc gggaccactc
     3061 cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttagca gggctgggga
     3121 tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc ggttttactc
     3181 cggtatcaca tgtaaccaca gcgtgtcgcc ttccatgccg ctggcgcggc atcagaataa
     3241 tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt cgtttcaggg
     3301 atatcgttaa gacttgagcg ataggaatta aaaccccaaa aagattaaaa aaacaccaca
     3361 aaacggatgt ttcttcaaca ccacttttgc tacatatgaa cggaaccgac gattaaactg
     3421 gatggctctg attgattcag ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg
     3481 gacgcaactt cccctctgcg gctctcagcc gcaccaccgc atccgggcca gcggctcatg
     3541 catcaggacc tgctctgcca gacggtagcc ccgcttcagc cccgtaaaac gcatctgact
     3601 ccc
//
LOCUS       DADWTH010000221         3596 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.227, whole genome shotgun sequence.
ACCESSION   DADWTH010000221 DADWTH010000000
VERSION     DADWTH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.227"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(334..600)
                     /gene="ospE2"
                     /locus_tag="JNY02_004149"
     CDS             complement(334..600)
                     /gene="ospE2"
                     /locus_tag="JNY02_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HBB9435427.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(782..1183)
                     /locus_tag="JNY02_004150"
                     /pseudo
     CDS             complement(782..1183)
                     /locus_tag="JNY02_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1612..2400
                     /gene="virF"
                     /locus_tag="JNY02_004151"
     CDS             1612..2400
                     /gene="virF"
                     /locus_tag="JNY02_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HBB9435428.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(2393..2764)
                     /locus_tag="JNY02_004152"
                     /pseudo
     CDS             complement(2393..2764)
                     /locus_tag="JNY02_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2763..3517
                     /locus_tag="JNY02_004153"
                     /pseudo
     CDS             2763..3517
                     /locus_tag="JNY02_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1056 a          697 c          736 g         1107 t
ORIGIN      
        1 gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat atatttctcc
       61 atctatgact tgtcaatacc atcctgatat ttgtgggtca gcaatatatt ctactaaaag
      121 gtatttaagg tcattacgtc cctgcactcg aataatggtg aagaaagcat gataaatata
      181 tcacgataac gttcagtatg tatcgaggat agatggagtt actcctctct gaaatagaat
      241 tactgctcta ctttttctgt ttccagaaat tattagtaac aaaccctgat atattatttt
      301 gaaaatggag cttaaaccag tctgaaatta acttcagaaa tcagaatacc gttggctttg
      361 tttttcatca attccggctt catttatctt tgtcaaccat tgcatttctg tagcagtgaa
      421 tttcaacgtt ttcgtccggt tggcagcagg tatttgtaat tcgtatatag ctgctttttt
      481 attgaaaaca gtatatccat ctgtagtaac agtagaagat aataatacgg gatttgatac
      541 ttcaaggatg atattttctt gtttttgtgg aaggcaaggg aatatagttt gtgtaagcat
      601 attgcttcct tatgttatat tttagtaaag aatttatttt ttacattata gtcggctaaa
      661 tgaagtttac tgtaaaaaaa cgatactttt ttcgtaagcg atgagatgtg gagccagaaa
      721 aatggctgcg ttacgtcatt gaactgtcct tgacccacga tattcagaca gtcgttgtat
      781 cttaagttga cattacccgc tgacgtcgat attcccttgg tgagagataa cccaacgcac
      841 tatgcggatg gttttcattg taatgctcga tcgccactgc aaggttatgc aatgccgttc
      901 ttggtttcgg catgaacgcg atgcagtctt ccttcatcgt tttcacgaac cgttctgcta
      961 ttccattact ctgagggcta ctgactgccg tcgtgcaggg ctccagattc aactctctgg
     1021 cgaactgccg tgtttcatga gccgtataag ccgaaccgtt atcggccact ggattggccg
     1081 ttccggtcct ttatcgccat atcgctcctg acatgacatc ctgcaccgtt gctttgtcat
     1141 aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga acgtaacgct
     1201 gtcatcggga gaatcgtcaa tgcggcattc ggttaacgct taccaatatc aacaccaacc
     1261 ggatttgtgg agcctccagt ctgaaggggc tttatgcgtt ccgtatagga tattatgatg
     1321 ctggagtttt gcgaagctcc actcctttcc gaacatttac acagatattg cctaagaaaa
     1381 gtgaaaccat aatatggtta tagtcccttt cagtgcaaat acttagcttg ttgcacagag
     1441 aaatagaagc tgcataagct ctttcttcaa aaaatgtaaa taaagttaaa tataggaaaa
     1501 attacttaat ctatcttaat aacggaaaga ttttgtatac aatcactgtt acagaaattt
     1561 cttagttact ctgtaaacac taaatatagt ttggtatatt ctgttgaatt tatgatggat
     1621 atgggacata aaaacaaaat agatataaag gttcgcttgc ataactatat tattttatat
     1681 gcaaaaaggt gttcaatgac ggttagctca ggcaatgaaa ctttgactat cgatgaaggg
     1741 caaattgctt ttatagagcg aaatatacaa ataaacgtct ccataaaaaa atctgatagc
     1801 attaatccat ttgagattat aagccttgac agaaatttat tattaagcat tattagaata
     1861 atggaaccaa tttattcatt tcaacactcc tattctgagg agaaaagggg gttaaacaaa
     1921 aaaatattcc tcctctctga ggaggaggtt tctatcgatt tgttcaaatc tataaaagag
     1981 atgcctttcg gcaaaagaaa gatctatagt ttagcttgcc ttttatcagc tgtttctgat
     2041 gaggaagctt tatatacttc gatatcgata gcttcttctc ttagtttttc tgatcagata
     2101 aggaagattg ttgaaaaaaa catcgagaag agatggcgtc tttctgatat ttcaaataac
     2161 ttgaatttat cagaaatagc tgttagaaaa cgattggaga gtgaaaaatt aacatttcaa
     2221 caaatccttc ttgatattcg catgcatcat gcagcaaagc ttttattgaa tagtcaaagc
     2281 tatattaatg atgtatcaag acttatcgga atatcaagcc catcttattt tataaggaaa
     2341 tttaatgaat attatggtat aactccaaag aaattttact tatatcataa aaaattttaa
     2401 atgcttcata gcccatcgct attgccagat gggttttccc cacacctgat ggccccagta
     2461 atacgatatt ttcattacgt tctatgacgc tgagtgagcg taacgactgg agttgcttct
     2521 gcggtgctcc ggtggcgaat gtgaagtcat actcttcgaa cgttttcacc gccgggaagg
     2581 ctgccattcg ggtatacatc gcctgtttac gttgatgacg tgccagtttt tcttcatgaa
     2641 gcagatgctc caggaagtcc atataactcc attcctggtc tactgcctgt tgtgacagcg
     2701 caggcgctgc gcttatgtac tgcacccatt ttgttggacg atgaaatgga atagcccctg
     2761 atatgtcaaa gccaaaatac ccttttgaaa agcgccttga agtcgtgaat cactacttca
     2821 ctactgatga tggttacagg atcatctcgg cacgttttgg tgtcccccga acccaggtca
     2881 ggacatgggt tgccctctat gaaaaacatg gagaaaaagg cttaattccc aaacctaaag
     2941 gtgttagtgc tgatccagag ttgcgtatta aggtcgtgaa agctgtgatt gagcagcaca
     3001 tgtcccttaa tcaggctgct gctcacttta tgcttgctgg tagtggttct gtagccaggt
     3061 ggctgaaggt ttatgaagag cgcggagaag ctggtttacg cgcgctcaag attggcacca
     3121 aaagaaacat tgcaatatca gttgatccag aaaaagcggc atcagcattg gagctgtcaa
     3181 aagaccgacg cattgaggat cttgaaaggc aagttcgatt tcttgaaacg cggcttgtgt
     3241 atctaaaaaa gctgaaagcc ttagctcatc ccacgaaaaa gtgaaagtac tcaacgagct
     3301 aaggcagttt tatcctcttg atgagcttct cagggctgcg gagataccgc gcagtacgtt
     3361 ttattaccat ctaaaggctc tcagcaagcc tgacaagtat gcggacgtta aaaagcgtat
     3421 tggtgagatt tatcatgaga atagaggccg atacggatac cgtagggtaa cgctgtctct
     3481 tcatcgagaa gggaaacaga ttaaccataa agctgttcac gtataagaaa ttgaaaaacc
     3541 agacgatacc gctgtccacg ccttatgcca cgtgagcttt tctgcttttc cctcca
//
LOCUS       DADWTH010000222         3542 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.228, whole genome shotgun sequence.
ACCESSION   DADWTH010000222 DADWTH010000000
VERSION     DADWTH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.228"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..221
                     /locus_tag="JNY02_004154"
     CDS             <1..221
                     /locus_tag="JNY02_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435429.1"
                     /translation="ANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGVG
                     PGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            938..1126
                     /locus_tag="JNY02_004155"
     CDS             938..1126
                     /locus_tag="JNY02_004155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435430.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1223..1402
                     /locus_tag="JNY02_004156"
     CDS             1223..1402
                     /locus_tag="JNY02_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435431.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1454..1648
                     /locus_tag="JNY02_004157"
     CDS             1454..1648
                     /locus_tag="JNY02_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435432.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(2447..2783)
                     /locus_tag="JNY02_004158"
                     /pseudo
     CDS             complement(2447..2783)
                     /locus_tag="JNY02_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1131 a          760 c          693 g          958 t
ORIGIN      
        1 tagccaattt tatcgcatta caaataaatg gttcaaaaac aggatttgcc gcatctatta
       61 caggttttac tcaaacaata agcgctggta caatcagttt cctgtcagta accgtaagcg
      121 acaacggtgt tggtcccggg actactttag gtcttggttc actaataact tcattactgt
      181 caatagccgt aatcgccatt gcaggaaata ataaaaagta agtgtactct gactgcgtat
      241 gtatctactt tgtgtaatgc aggtgtctat tgaaaataat tcttttcgtg ggcatctgtt
      301 gtataaaata aaaaattatc gtaagtactc tatctgcgat gtcttgtgaa aattatcctg
      361 tctggcaaca atcgcgtcca tctgtattaa tggacgcgaa cgatgaactc tcaaacaaca
      421 ctagatatca ccggctttcg cacttatttg ccagaatcat tacgtctcag attcgacgag
      481 caattatctg tccgggctat cgcacagcag ttaagactca gttattcccc cattcacaat
      541 ctctttcaac gaattaacgc ctcaggtatt gtctgaccat gacccgagtc cctttcattc
      601 gcccaaccga taccatactt tatgccaaca gaaagaatga atgaaccgcg cctaaaatca
      661 gcaaggacac atggcacaaa gtgcgacatg tctcacattt aaccccagtg ggggcaacaa
      721 gtataaaacc gttttttgat taagtggtcg aatatgaaaa ggggaaggtt tatgtgaaaa
      781 atacctgtgt tttctttgaa aacacacaac agtatcaggt tatttatgtt tatacagtgc
      841 gttccaaact gacctgaata attacgcaag ttgcaacaat aaaacatttt tgccgtagtg
      901 tgaggtactc cggtaaattg ctttccaggt gcagacaatg actaacgaag aactacaggc
      961 aatcgcatta tccatgcttg agcgccagcg cgaaaaagta caccagcaac gagagtcttt
     1021 gcttaaccca tctccattca ccaggccaaa acgattggag cttgaagcat tcttggacaa
     1081 atacccacgg cggcttaaat caggcaaaac cagaccacca ggctgatagc tcacagtttg
     1141 ttctccgatt caataaaacg gataagaacc atcagcgcgg tagcgtaagg cactacagcg
     1201 cattgcttta cgagtaataa caatggattt atcaccagaa gacgcaaagt ttttacgaga
     1261 actgcaacac cgtatgctca gagaagccat tatcgaacag caaacgggca tgagcgccat
     1321 agacccattg cgatacaaac gggcctcatc tcctttagtt gacgacaatt ccaatgcgga
     1381 taaaacacgt ccaccacact gattacattt ttcattagca cagtggtgtg atgcagcata
     1441 cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg gagcaactgt tacaggcacg
     1501 ccaacgggag tttgccacta aatggggacg tgccgcgctg ccacatgaac gcctaaccta
     1561 ttttccactt gaagacctca ttaaaaataa tggcaaaaaa atcaggtctg ataagaaaca
     1621 cttcttagaa tgcaatgaag acaaataaca acaatctgca tattgccacc aaccacagat
     1681 gtggcatcgt tatcagcgca cttgattttc tttatatcca ctgacgagct tgccggggca
     1741 cctttgtttg ccggtcattc tggtatcatg cagatattta cggcgtacaa gcccactagc
     1801 atgacattgt aataaggcct gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt
     1861 aatcgttgac aatatggtgc atgtaatcgt aagcgtccag cgaaatccat ctggtcataa
     1921 tctgaagcat ccgacaaagt ggtgtccacc aaataagtga tctgcgccca atatcctgag
     1981 ccggaacttt gacaaaggcc aacgaaaaat gggagcggat gttacagagt tctcggtaca
     2041 ggggaaaagt ggtacctgtc gccagttctc cgatctgttt aaccgggaaa ttatctccta
     2101 cagcttgtca gaaaggtcgg tgatggagca tcgttaatac gatgctatac gatgcattca
     2161 cagtgcccgg ccagaggatg ccccgtcgct gcatatggat cagggttggc aatatcgaat
     2221 cgcaggctat aggcaaagtt aaagccccat ggagtagcac aaaatatgcc gtacaaagga
     2281 aacggtctga ataacgcagt gatgaagaac ttcttcagca cactgctaaa acgcatagtg
     2341 atcgagcgct gattctggcg aacaactgaa ctaatacatc agaatctgca ttatgttaaa
     2401 taaatatgaa aagatggttt aaataccccg ttacttttga cttactctat acggtatcgc
     2461 atcgtttaat attcgcaccg gccagatttt tatttctatt agttgtcaca atactgaatg
     2521 cgtacgacca cagtattctg gctcctgtgt ggttatgctt taattctgcg ttccgggcag
     2581 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt tttaccagtg
     2641 ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat agcattgtcc
     2701 ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt ccgtttttca
     2761 taacccactg tactgataac cataatctaa tcagtagaaa ttgagtcgaa aataagcact
     2821 actccataca ggataattag aggtcagttt gattattcac aattcatcat cagcattttc
     2881 tatttctgac gaaatcaaca tgaaaataac catatatgat aattattata ataacggctt
     2941 taattggaat acatatatta caacgtatta tatataattg gtattctggg aactatattc
     3001 tcaaaataca gtagaaacga ggtatgtttc tggtggaaag gacagtggga ttaaaaagta
     3061 agaattgata aaaaaaacgc cagcaacaca tagtgccggc ggagggaata ccccatggag
     3121 aaaatgtgat gcctagagca tcatgaatat ataattaaaa atagttagcg ttgtcaatac
     3181 tttcacaaaa ataattttcg tagtatagaa agatattttt atgcatgacc tacctgaatt
     3241 tgctccgggt agaggttata aataaaaatt gaatcacgac aaacacaata ttcatagtat
     3301 ggcgatgcct acgccagcaa gaatagcgcc aataatattg ggaatataat agacaccaga
     3361 cgcacaggca tctcactcct taacaaacaa taatcaggat atttactttt acccagctaa
     3421 ctgtttacac ccaaagtgca cacaattaac cattcaataa caaatggcaa tatccatagc
     3481 catacgactt taccttgtaa tgttcggtat ttctttataa ttattctggg aaatctatca
     3541 tt
//
LOCUS       DADWTH010000223         3519 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.229, whole genome shotgun sequence.
ACCESSION   DADWTH010000223 DADWTH010000000
VERSION     DADWTH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.229"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNY02_004159"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNY02_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBB9435433.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNY02_004160"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNY02_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBB9435434.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3519
                     /gene="cusC"
                     /locus_tag="JNY02_004161"
     CDS             2335..>3519
                     /gene="cusC"
                     /locus_tag="JNY02_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBB9435435.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEKAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQ"
BASE COUNT          864 a          911 c          922 g          822 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaagcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaaa aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
     3241 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
     3301 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaattga gatccccatt
     3361 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
     3421 gtggtgaatt atgaacagaa aatccagaac gcctttaaag aggtggcaga tgcgctggca
     3481 ttacgtcaaa gcctggacga tcaaatcagc gcccagcag
//
LOCUS       DADWTH010000224         3411 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.230, whole genome shotgun sequence.
ACCESSION   DADWTH010000224 DADWTH010000000
VERSION     DADWTH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.230"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            88..494
                     /locus_tag="JNY02_004162"
                     /pseudo
     CDS             88..494
                     /locus_tag="JNY02_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1078..1794
                     /locus_tag="JNY02_004163"
     CDS             1078..1794
                     /locus_tag="JNY02_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435436.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2346..2750
                     /locus_tag="JNY02_004164"
     CDS             2346..2750
                     /locus_tag="JNY02_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435437.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAIPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2813..3411
                     /locus_tag="JNY02_004165"
                     /pseudo
     CDS             2813..3411
                     /locus_tag="JNY02_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          952 a          706 c          795 g          958 t
ORIGIN      
        1 caaccgggag cgctttaagc tgggagtgat gttccactat gggaaactga atatggcgtt
       61 ctaagccttc ccaccatgat cggggaagac ccagatatga acaggcacat tcagagttac
      121 tttaaagagc ttactgaacg tctgaggtgt tagagccatg actgccgcag cattgctggc
      181 ggaaatgcct gaatcgagca gcctctcgag acgtgaaatc agcgcacttg tcggtgttgc
      241 tcaggtaaac cgggactcgg gaactctgcg cggtcgacga accattttcg gtgattgtgc
      301 tggggaagaa cagctctgta catggcggcg cttgaggccc ccggtttaat ctggtgataa
      361 aggcgtttta catgcgccta cttgcagccg gaaacgccaa aaaagtggca ctggttgcct
      421 gtatgcgtaa gttgctgacc attatgaacg caatgctcag aaaaaacgaa gaatggaatg
      481 aatcgtattt atgaaaactg gattgtgggc cataaatatt ccaaaaagat agcccgctcc
      541 gtttattacc ggaacgggcg agagcatgac attgtctgtt attataatga gactgctgat
      601 tctacttatt tttaatttca agaaaagcgt tttctacaaa gaattatgag atttaatgtt
      661 ttgtttagac aatattatca ttctgttcaa taatgaaagt cttcatcaac aacaagtaaa
      721 aacaacatcc ctctcatcga accggacttg cagatttccc gcatccggct ctcttgcaac
      781 acatcagacc ttcacgttcg ggcggttatg gggaaggtcc agcaagctgt atgagcccta
      841 actgtttgaa taccagtaga ttaccagaaa cttctatcga ttgaagacat taccaactag
      901 cgttactaaa tgttgaccaa gttttgttca cttgtctggt tttgatagta gcattctgca
      961 gagtattttg atatgataaa tatgcattaa caatgatatt attgttaatt tcctagcata
     1021 aaaattaaaa gatcaacttt tatctcgaat attttttaca ttatcagttg gtttcttatg
     1081 aatgacaatt cattgcttag aaattcttca ttattcatcg cctatatggg atgtgtaggg
     1141 tgggtcagtg cttattctta tggctgggga acctcttttt actatggatt tccatggtgg
     1201 gttgttggtg caggtttgga tgatgttgcc agaagtttac tctatgctat catagtaatg
     1261 ggtatacttt ttaccggttg ggggattggt gttttatttt ttcttcttat aaagaaaaga
     1321 agtaaaatac aagacttgag tttttttaga ctattttttg ctattaccct actgtttttc
     1381 cctgtaatat tcgaactttt aattctcaaa caatatttca tacttccatt atcactttca
     1441 tgcattatta gttcattggt tatttcaata ataattcgta tatatggacg gatcttttct
     1501 gtatcatgtt tttcagatat cccttttgtt agaaaacacc gcattaagtt aattatggct
     1561 ggttttttgg tttatttttg gcttttttcc tttcttgttg gctggtataa acctcaatta
     1621 aaaaaagaat atcagatgct ttgttataat aatagttggt attatgttct tgctcgttat
     1681 gattcacggc tcgttttgtc tagttcattt aaagatgaca gtaacagatt cttaatattt
     1741 aatacagaac agtcgggatt ttatgaaatc aatgatgttt atgtcagaaa gtaataatcc
     1801 tctcccacaa agtgaattaa atgaatatat aatgcgttcg aagtatatgt attgagctgt
     1861 catactgaga aacaaacctt agggcagcac aattaaagcc agaaacaact atgcaagaaa
     1921 gttactaaat aagtgctcgg aacagtaaaa cactcagggg ggactcagtc cggattatgt
     1981 aatcagataa attgccaaac atcacaaatc accagccgga ctgagtgatg ccaagcattg
     2041 caccaatacc ccgtgatgaa cgatgcctga tgcagaaagc tccctataaa acaaagaaca
     2101 aaaactacga ccggcgattc accgtcatac tgatgctgca tcgtggtgac actgtctgtc
     2161 actttcatgt tgactgaaca actctgctgc gaagaaggtg taccgccggc gggatttgat
     2221 tgtccggcgt ctagcttcca cttccctgat ggaactgaaa gttgttatgg ccggtcagct
     2281 tgccctgtcc gcaggacatg aggtgctgtg acggacagca ccagtaacgg aaagtgacaa
     2341 tgaaaatgaa tactgcgctg atagttgcac taatgtgtat gtggtatgcg gtacccgctg
     2401 ctgcaaaaga gactttactg gctataccac gtaattcaac agaacattgc tatgcggaga
     2461 ttaatgtgca cggaccatat ggggtgtatt tccgagtggt cccgcatccc cccgggggaa
     2521 agagctgggt cgaatgtaac agtgactact attactctga taaaccaccg ggagtacaga
     2581 tactcggaac ccgtgcaggg tgtcgggtat acggaatctg cgggaccacc tcgacactgc
     2641 atgtggctgg aagaggggtt gtgtgtatta aaaatatatg cagtccacgt gggatgataa
     2701 ttcacaggat acggaaacgt cctgtagtgg ctgtaagcga tgagatgtga tcttacccag
     2761 caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc acatgacaaa
     2821 aaccgtatca accagcaaaa aaacacgtaa acagcattcg cctgaatttt gcagtgaagc
     2881 cctgaagctt gctgaacgca tcggtgttgc tgccgcagcc cgtgaactca gcctgtatga
     2941 atctcagctc tatgcgtggc gcagcaaact gcagcaacag atgacctcgt ctgagcgtga
     3001 aagtgaactg gccgctgaaa atgcccgcct gaagcggcaa ctggcggagc aggcagagga
     3061 actggctatc ctccaaaagg ccgcgacata ctttgcgaag cgcctgaaat gaagtatgtc
     3121 tttattgaaa aacatcaggc tgagttcagc atcaaggcaa tgtgccgcgt gctccgggtg
     3181 gcccgcagcg gctggtatac gtggtgtcag cggcggacag gaataagccc tcgtcagcag
     3241 ttccgtcagc actgcgacag cgttgtcctc gctgcggcct ttacccggtc aaaacagcgt
     3301 tacggtgcac cacgcctgac ggatgaactg cgtgctcagg gttaccactt taacgtaaaa
     3361 accgtggcgg caagcctgcg ccgtcagggg ctgagggcaa aggcctcccg g
//
LOCUS       DADWTH010000225         3388 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.231, whole genome shotgun sequence.
ACCESSION   DADWTH010000225 DADWTH010000000
VERSION     DADWTH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.231"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(185..880)
                     /locus_tag="JNY02_004166"
     CDS             complement(185..880)
                     /locus_tag="JNY02_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBB9435438.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(873..2300)
                     /locus_tag="JNY02_004167"
     CDS             complement(873..2300)
                     /locus_tag="JNY02_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBB9435439.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2311..3030)
                     /locus_tag="JNY02_004168"
     CDS             complement(2311..3030)
                     /locus_tag="JNY02_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBB9435440.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          847 a          945 c          708 g          888 t
ORIGIN      
        1 ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg taaatatcct ggcattacta
       61 atgcctggat atttacagaa gagcagcata accatgacgg aacggggtta agtcaggttg
      121 tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt ttatgttcag atctcatatc
      181 tctctcagca atcctcaata atcagataca ccgctttcac cggaccatga accccgacga
      241 ctttgataag ctcaatatcc gccgttgagc tgggaccact gatgatgtta atacatgacg
      301 gcattcgttc accggcctgc gctttctgat gcaatttttc tgcgagttgc gctacacgcg
      361 gcaggattgt gcttttacgc aggataaaaa gagaagattc cgggagcagg ctcaatgaac
      421 gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa ccatattcag
      481 catacacgac acccacttta gcctgctctg cctgcgagat attctcggca cctttcgccg
      541 gatcccaaac aacggcattg cattcctgct gcaaacgttc actaatcccc aattcctcca
      601 gcctcgtgtc accgctaatc acgaccgact gatctcccag ctctttacac agacgtattg
      661 cagcttctgc cgccttcgcc tcgctggtca gctcacagcg cgtcaacata acatcgctgg
      721 caaactgaat aaacgcgtca cagcgctgct gttggttaag ttgggtaagc cgctcgttag
      781 catagttgtt aagcggcgca tcttctgctt gcggttcaag tcgcagcggg cgacccagtg
      841 cctgagcaac gttattcaaa aattcgctcc gattatccat tctttttctc ctgcgcctga
      901 tgtttcttaa accaactacg gaaactctct ccgtcagctt caggaagatc gcgtgcttcc
      961 atccagtcgc taatcgcgcc aaatttgagt ggtgttttac cgccattgat aaaccagctt
     1021 gccgcatgag cgccggccat catcccgact ttccacaatc ctggatgact attggcataa
     1081 gcgaacattt ttatcgcccg ttgctctgct tttgcggtga tccctttttc agccatcacc
     1141 cgacgatgac gcaaaatcag ttttgacagc ggaatacgca ccggacacac gctgtcacaa
     1201 gcagtgcata aagagcaggc gtagggtaaa tctttaaaat ctttatagcc gccaagtagc
     1261 ggagaaatca ccgcaccaat tggccctgga taaatagagc catatccatg accgccaata
     1321 tggcgatatg ccggacaagt attcatacaa gccccgcagc gaatacagcg cagcacatcc
     1381 cgaaattcag aggccagcac ctcagaacgc ccgttatcga caataaccag atgaaactct
     1441 tcaggaccat caacgtgccc cgcttcgcgc ggccctgtca gccaggtgtt gtatcccgtc
     1501 aaacgtgcac caacggcact gcgcgccagc atggtgatca atacatctac ctcggcaaac
     1561 gtgggggcaa tacgctccat tcccatcact gcaatatgcg ttttaggcag cgtggtacac
     1621 attcgcgcat taccttcatt ggtcaccagg cataccgaac cggtctctgc caccgcgaaa
     1681 ttacagccgg taatacctat ttcagcactg aggaaatctt cacggatttt ttgccggatg
     1741 aataaggtca tcgcttcagg cgtttccggc ccctcatagc ccagacgttc gtgtagcact
     1801 cgacggatct gatggcgatc tttatgaatt gccgggacca caacatgaga tggcggatct
     1861 tgatccagct ggagaatata ttcacccaga tcggtttcaa tcacctgaat gccagcatcc
     1921 tgcaacacat gattgacacc aatctcttcg gtcaccatcg atttagattt caccaccttc
     1981 cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc tttggttttt
     2041 gcaaaataga cgtgaccgcc gttttgcgtc actttttctg agagctggta cagataagcg
     2101 tcgagattac tcaaaacatg atcacgtatc tgggcggccc gatcgcgcca ctcctcccag
     2161 tgccccaatt catcgaccat tttttgccga tttgccccaa tacgctgctg cgcgtttgcc
     2221 accgctttgc gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta
     2281 ttactggttt tgatcgacat ctttatatcc tcagcggctc atcaacactt cagcaatatg
     2341 catcactttg actttctgcc cttcccgttg taatcgccca ctgatgttca gcaggcaact
     2401 cacgtcagca ccaattaaat actcagggcg gacttccatc aggtgcacaa ccttttcttt
     2461 caccatctcg ccggatattt cggccatttt gaccgagaac gtgccgccaa atccgcagca
     2521 ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc acatttttca gcagcgtaag
     2581 tggctcgtcc ttcactccca gcttacgggc caggctacaa gatgggtgat acacagctct
     2641 cccttgcaaa ctggcaccta catcgactac ccctaattta ttaacaataa aagaggtgag
     2701 atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt catccgccag
     2761 atacgtcggg taacttttta cggcataggt gcaagagcca gccggtgaaa taatgggatc
     2821 gtcgttatcc tccagtgcgg cgatcagatt tttcatccct ggaatcgctt ctttgatata
     2881 accgctattg atcgcaggct gaccgcagca tccctgtttc tccgggaaat ttacgcgaca
     2941 gccgagtttt tccagtagca gcacggagtc tcgtgccatt cttgatttca gggcgtcacc
     3001 aatacaggtg acaaagaaat tgacattcac aactaatact ccattacttc atgcccattt
     3061 atgcccgatg tttccccccc accgggggat caatcatcaa taaatatggc gttcttttta
     3121 aattggctag caacatacca cccctcattt gtatgacaaa tgataatctt tcatccaaac
     3181 ttcaaatgca ataactaatt ttatttcttt cggcatcgct cacattttat ggggaaatcg
     3241 acgatcattt ttggcataag aaatccattt aaaaatggct acatccctta tacctattca
     3301 gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaaa aatcaccgac
     3361 aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DADWTH010000226         3355 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.232, whole genome shotgun sequence.
ACCESSION   DADWTH010000226 DADWTH010000000
VERSION     DADWTH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.232"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            107..418
                     /locus_tag="JNY02_004169"
     CDS             107..418
                     /locus_tag="JNY02_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HBB9435441.1"
                     /translation="MDAHWKTVLKMSVKRWLWLIIVSVLMFATTGSLLWCQGMKINAN
                     MNILREQKESLEKLNAKTWGVRYNEDSNGRFLVLPEGMKAETNWTMENGKQNAVRLVQ
                     E"
     gene            425..658
                     /locus_tag="JNY02_004170"
     CDS             425..658
                     /locus_tag="JNY02_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBB9435442.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1237..2355)
                     /locus_tag="JNY02_004171"
     CDS             complement(1237..2355)
                     /locus_tag="JNY02_004171"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBB9435443.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2473..2562)
                     /locus_tag="JNY02_004172"
     CDS             complement(2473..2562)
                     /locus_tag="JNY02_004172"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435444.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(2998..3159)
                     /locus_tag="JNY02_004173"
     CDS             complement(2998..3159)
                     /locus_tag="JNY02_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBB9435445.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT         1018 a          635 c          773 g          929 t
ORIGIN      
        1 cagggaactg acctcaaacg aatctttaat caggaacgct atcaacgacc atacatcagc
       61 cctgaaagaa cttctggaga ggtatcagaa aacgaccgtc gatacgatgg acgcacactg
      121 gaaaaccgta ctgaaaatga gcgtgaaacg ctggctgtgg ctgattatcg tctcagtact
      181 gatgtttgcc acgaccggga gcctgctgtg gtgccagggc atgaagataa acgcgaacat
      241 gaatattctc cgcgagcaga aagagagtct ggagaagctg aacgcgaaga catggggcgt
      301 gcgttataac gaggacagca acggacgttt tctggtgttg cccgagggga tgaaagccga
      361 aacgaactgg acgatggaaa acgggaaaca gaatgccgta aggctggtac aggagtaaca
      421 gagcatgacg gagctggaaa gacatttgct gaacgcatta gagcaactgc aacaggacta
      481 tatgcagcgg ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa tgttttcgct
      541 tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg cagttgagtc agcaggtgca
      601 gcacttgagc gagcagacag aacgcttgag ccagttatac agcgagaaca ggagataagg
      661 gacgaacggc tgatacagga atgcgaacat gcgttatccc tggagcgtga gcgacagctg
      721 gaaatacagg aacggacact ggatggccct tcgctgggat ggtaatcaga gacaggctaa
      781 aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat ggttggtttg ggggcaccgg
      841 gaacgcgctt cagtcagacc ttgctgaaaa cataggaacg cgttaaaacg catacgggcg
      901 cgattctggt gtgtcttttg ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc
      961 cctgaacttc tgttttcccg ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa
     1021 aatccgtttc agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta
     1081 tgcatgtggt taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca
     1141 tttttacctg tctgctgccg tgatcgcgcc gaacgcgttt cagcggtgcg tacaatgaga
     1201 tgttatggta aattcaagtc ttagatttta tgatgtttaa ttagcttttt ttgcgtttat
     1261 aatgttcatc ttttttatta aaattgattc aatctttgat gtgggtatta atattttctt
     1321 tgacaaatag aaagttaaaa aaaacaccat taatagtata aataaatacc caaaaacaga
     1381 agcgttatta atttcaatta ttgatgttaa tctgtatatt actacatgct gaatcaacat
     1441 ggatatgtag atgcatttag catatgagtt gaaaaaatag aggttaaatt tcttattaat
     1501 tattaatgct gctttgtaaa tgataatgct tatgcacatt aaaggtatta ttgataactg
     1561 gtgattgact atgtttgttt tgaactcatt aggccctatt aataatactg tcaatataca
     1621 tataacaacc gatagaataa ctgtggtgtt tttttgtaat ttatttttgt atttaaatgc
     1681 aagcatccca accataaact caggaagtcg gcaagcaaaa aaccaaaatg ggaagtttaa
     1741 aaatgatgat gagatatttg atatcgggaa tgttgtaatt gttatcacaa ataaaaaaaa
     1801 tatgttacgt ttaaatgtaa aggtattcat aacgtgaaaa actatcggtg atatcacgta
     1861 cagcataata acgcacccaa taaaccactc gcctgtaagg aaaagaaaat cataagagaa
     1921 atgggcgttt aatataccat caagggcaaa aaatactgga attattctag tgtactggcc
     1981 tcctgttgtc attattgata cgagagggac tgtattaatt atctctttat ttaaataaga
     2041 caataaaatt aagagtaaag agataaaaaa gtaagtgata atataaggag taaggatcct
     2101 tactcctttg tcaaggaaat aatcaatggt gttctttcta tctagtgaaa taaatgccaa
     2161 atatccagat ataaaaaaaa acaagctcac acctacctgg ccaagaaccc ctccataaaa
     2221 tgatggtatg aaagatagcc tggtttggct aaatatgtag ccataatgag atataataac
     2281 aaaaatgcag gctgttactc gcaatgtatc aattgacgga tctctattag acataacctt
     2341 tatttcgctg ttcaaattat cgcctttagt tgatgatgta aaattaacgc tataaataag
     2401 ctacacattc tagagcagcg aaaggatgat tcaagtattt ttaataataa aacgcattga
     2461 taatgtttat gttcagatgg tgctgatagc tttgtcatgc tgttccactg atgttgataa
     2521 cgacagagac ttttgtcctt ttcttgttag atgctgcctc agattcggat tatggcgtta
     2581 aaggggtttg acgctcaacc gaacggtttc acacgttaag agctttttgt ctgataaatc
     2641 aaaatataat ggacattgaa aaacgccctc aaaacggtgt ctttttaatc gaagattggc
     2701 acaaaacgga caccaccgtc cattagtcga ttttttagac ggcttttctc gcctaacgtg
     2761 agttttcgtt cggttgagca tcagacccct tttatattgt agatttctat ttttagacag
     2821 ttggaacagg acaattaaca acagatctac ttgagatcat ttgcttgtcg tcgtagtctc
     2881 tttcccagaa acgaaaaaac cgccctgcaa ggcggtttct cgaaggttct cagagcgacc
     2941 aactctttga accaaggtaa ctggcttatg aggagcgcag taaccaaatc tgtcctttca
     3001 gtttagcctt aacaggcgca taacttcaag actaactcct ctaaatccgt taccaatggc
     3061 taccgccact ggcgatttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     3121 acgggcgtgg tagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     3181 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
     3241 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcg
//
LOCUS       DADWTH010000227         3239 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.233, whole genome shotgun sequence.
ACCESSION   DADWTH010000227 DADWTH010000000
VERSION     DADWTH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.233"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..328)
                     /locus_tag="JNY02_004174"
     CDS             complement(<1..328)
                     /locus_tag="JNY02_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB9435446.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENI"
     gene            574..1335
                     /locus_tag="JNY02_004175"
     CDS             574..1335
                     /locus_tag="JNY02_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB9435447.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(1442..1942)
                     /locus_tag="JNY02_004176"
     CDS             complement(1442..1942)
                     /locus_tag="JNY02_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435448.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(2093..3239)
                     /locus_tag="JNY02_004177"
                     /pseudo
     CDS             complement(2093..3239)
                     /locus_tag="JNY02_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          973 a          724 c          579 g          963 t
ORIGIN      
        1 ttatgttttc actgatgtaa aagcctactt tgggtatcgt aagaatcaca ttttcttcaa
       61 caccaatatt tttaaaactt ttgcgtaaca gggatatata ttggttcaat gtgttgtttg
      121 acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg tcaaccgctc
      181 caccattact ttccagtaac actgccaaaa tacaccccgc cgtagctggc aaatttatct
      241 catcctgggt atttacattc gagagcgagc atttgttcgg atcaaaaata atcgcgtcat
      301 tgataatgaa aaaacatctc cgctccatgc aggcaacaac acaaaataag attttgtcat
      361 tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata accctgaaaa
      421 agtcatataa aacttatatc cacagaataa aaaaccaaac atgtcagttt tatcaatata
      481 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
      541 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
      601 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
      661 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
      721 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
      781 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
      841 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
      901 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
      961 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
     1021 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
     1081 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
     1141 gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
     1201 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
     1261 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
     1321 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
     1381 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
     1441 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
     1501 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
     1561 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
     1621 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
     1681 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
     1741 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
     1801 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
     1861 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
     1921 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
     1981 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
     2041 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
     2101 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
     2161 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
     2221 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
     2281 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
     2341 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
     2401 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
     2461 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
     2521 tactcagtcc cggctagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
     2581 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
     2641 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
     2701 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
     2761 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
     2821 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
     2881 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttccgat
     2941 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
     3001 tgcctctcca taccgtacta taactcagtc tggtagaccg cgtgtttcct gtgccccggg
     3061 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
     3121 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
     3181 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagac
//
LOCUS       DADWTH010000228         3142 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.234, whole genome shotgun sequence.
ACCESSION   DADWTH010000228 DADWTH010000000
VERSION     DADWTH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.234"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNY02_004178"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNY02_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBB9435449.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="JNY02_004179"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="JNY02_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNY02_004180"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNY02_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBB9435450.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNY02_004181"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNY02_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBB9435451.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="JNY02_004182"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="JNY02_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          761 g          838 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc tctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcgaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccaacgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DADWTH010000229         3135 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.235, whole genome shotgun sequence.
ACCESSION   DADWTH010000229 DADWTH010000000
VERSION     DADWTH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.235"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            805..999
                     /locus_tag="JNY02_004183"
     CDS             805..999
                     /locus_tag="JNY02_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBB9435452.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESETGT"
     gene            complement(2024..3133)
                     /gene="fepE"
                     /locus_tag="JNY02_004184"
     CDS             complement(2024..3133)
                     /gene="fepE"
                     /locus_tag="JNY02_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBB9435453.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          928 a          773 c          653 g          781 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccccctaaaa agcggtgttg gcgcagctac
      421 aacaccgcgc cgacaccgct tttttaaata tcacaaagag agcaagaaga actaatttgt
      481 cataactctc tatttataaa gaaaaatcag aaaaaacttg tttttgcgtg gggtgtgggc
      541 atttcaggca tgagaacaga ctcgcgtttt tccggagtat cagcggaatt attgattaag
      601 aaacactcga cgcagagcga aattttatgc aacagcggct gcactgcgca gggattattt
      661 atcagttgat atgctgtgcc atgatacgtc ctaaagaaac aggacaggat gtgcaattgt
      721 aataccgtcg cccgcaacgt cttacaatta ccacctgctc agggcccccc cccgacaccc
      781 ggaactgacg gagaacaacg gaaggtgaac aaacaacaac aaactgcgct gaatatggcg
      841 cgatgtatca aaagccagag cctgacactg cttgaaaaac tggatgctct ggatgccgac
      901 gaacaggcgg ccatgtgcaa acgactgcac gaactcgcag aagaactcca gaacagcatc
      961 cagattcgct ttgaggccga aagtgaaaca ggaacataac gaagctgacg gaggcggtca
     1021 cggctttccc gtgggcgttt tcgtcagcag acaagcccgt cagggcgcgt cagggggttt
     1081 tagcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tatatactgg
     1141 cttaatcatg cggcggcaga gcagatggtg agagaacagc gacatatgag gtgtgaaatg
     1201 ctgcacagat acgtaaggag aaaatacagc ttccggcgct cttccgcttc ctcgctcact
     1261 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtgt ctgctcacgc aaagacggat
     1321 atacggttat ccacagaatc aggggataac accagaaaaa acatgtgagc aaaaaagagg
     1381 ccccgaaaaa aggccgcgcc ggtggcgttt ttccataggc tccgcccccc tgacaagcat
     1441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggacttaa agataccagg
     1501 cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     1561 tcattccgct gttatggccg cgttcgtctc attccacgcc tgacactcag ttccgggtag
     1621 gcagttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1681 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttaac gccattggca
     1741 gcggccactg gtaaacagga tgtgcgagag atgtattgag agttcttgaa gtggcagcct
     1801 tactgaggct acactagaag gacagatttg gtgacatgtc tcgcacaaga ctgttaccac
     1861 ggttcagaag ttccccaact tactgaacct tcgaaaaacc acctccccag gtggtttttt
     1921 cgttttctag agtagaagac tacggctgta agggaatgat ctcaagaaga tcctttgaac
     1981 tttttataaa agttgcttat aaaatgatgc atcagcacaa cttttactca atcacactca
     2041 tctttctggc ctccatggca tgacgtaaaa gtatatagcc acatgcaagc atccctccaa
     2101 tgacagaaga aagaattaca attatggcat tacttggccc atcctttctg accggtaacg
     2161 ttgggctcaa ttgatactga aatggcataa aatcaacatc ccccactttc gccacaacca
     2221 attgattcac aacatattga cgatttcgca actccccact caattccgaa acatccttga
     2281 ttgatttttt gatgttcaat ttagttgcta taccatcaga accgagagcc acaggaaaat
     2341 ctgggtcatc cttcataatc tgaccattgc tgtatacagg tttttttatt ccagccgcat
     2401 tagcaacctc cagtgaataa ttgaggcgtc gaatatctgc attcagctgg tttctaattt
     2461 ttatctcatc cagtgcaaga atttcttttt caacattagt tttaacttct agtttatttc
     2521 ttatatcttc catcaaatcc cttacaacaa gggaagaaac ataattaata tacccagaca
     2581 acacatcgtg agcttcttta cttgttggag cagtaaaact cagagtccat gatacataca
     2641 atgatgactt gtcgtttttc ttactggcat tatcatcaat cgacttcata tttttactca
     2701 actcaacaat tgcacgatgc agttcgagag cgctaatgtt attcttttta ataatctcca
     2761 taacataagg agatgatctc atatactcat tcaacgaatt aacggactga aatttcttaa
     2821 taaacagagc aaatgcgcta tcacgattga tatcaaaatc cactcccaga acatgaagct
     2881 tggaaagctc cttatttaat tcactccatt gaacagactc agcaggagta accacagcag
     2941 aactagtcca tttttgtggc aaaacaaaag aaacaagaaa gccaatacaa gcaaaaaata
     3001 gaatcacccc aacaattttt atttttgctg cccataaaac gctaatcaag ccaaaaaggt
     3061 caatttcttc attaccattc ggcaacatgt tgtaattaga aaaatgttcg tcagagacct
     3121 ttgagatttt cattg
//
LOCUS       DADWTH010000230         3058 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.236, whole genome shotgun sequence.
ACCESSION   DADWTH010000230 DADWTH010000000
VERSION     DADWTH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.236"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            238..2106
                     /gene="kup"
                     /locus_tag="JNY02_004185"
     CDS             238..2106
                     /gene="kup"
                     /locus_tag="JNY02_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBB9435454.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2273..2692
                     /gene="rbsD"
                     /locus_tag="JNY02_004186"
     CDS             2273..2692
                     /gene="rbsD"
                     /locus_tag="JNY02_004186"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBB9435455.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2700..>3058
                     /locus_tag="JNY02_004187"
     CDS             2700..>3058
                     /locus_tag="JNY02_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB9435456.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          689 a          729 c          806 g          834 t
ORIGIN      
        1 gaagggcctt cccgagagtg gtaaaggaga ggccctggaa ataccccaca gccccaaaaa
       61 agagccagtc agttgcttaa ctgactggca ttactcaaag ggggggcttt ttctttgagc
      121 caggttaatg tggccgcatt taggagtacg attttgccgt taatcgtgca tactgtgcgc
      181 ttttttgtgg gccaagggac taagcacaca tttcatattt caacgaaaga ctagtctatg
      241 agcactgata ataagcaatc attgcccgcg attaccctcg cggcgattgg agttgtctac
      301 ggcgatattg gtaccagccc gttatataca cttcgtgaat gtttgtccgg ccagtttggt
      361 tttggcgttg aacgcgatgc cgtgtttggc tttttatcgc tgatcttctg gctgctaatc
      421 tttgtggttt ccattaaata tctcaccttc gtgatgcggg cagataacgc cggtgagggg
      481 gggatcctga cgttgatgtc gcttgccggg cgtaatacgt cggcgcgaac cacatcaatg
      541 ctggtgatta tggggctaat cggcggcagc tttttctatg gtgaagtcgt cataacaccc
      601 gctatttcgg tgatgtcagc cattgaaggt ctggaaatcg tcgccccgca gctggatacc
      661 tggatagttc ccctctcaat tatcgttctc actttattat ttatgattca aaagcatggc
      721 acggctatgg tcggtaagct gtttgcgccg atcatgctga cctggttttt gattctggca
      781 ggactggggt tacgtagcat tattgctaac ccggaagtgc tgcatgcgct gaatccgatg
      841 tgggcggtgc atttcttcct tgaatacaaa acggtttctt ttattgcatt aggggcagtg
      901 gtgctgtcga ttacgggggt cgaggcgctg tatgctgata tggggcactt tggtaagttc
      961 cctattcgcc tggcgtggtt caccgtcgta ttaccttcct tgacccttaa ttacttcggt
     1021 cagggagcgt tgttgttaaa gaacccggaa gcgattaaga acccgttctt cctgttggca
     1081 ccggactggg cgctgatccc actgctgatc atcgccgcac tggcgacggt aattgcctcg
     1141 caggcggtta tctctggcgt cttctcattg acgcgtcagg cggtacgtct gggatatttg
     1201 tcgccgatgc gcattattca cacctccgaa atggagtcag ggcaaatcta tattccgttt
     1261 gtgaactgga tgctctatgt cgcggtcgtg attgtgattg tcagctttga gcactccagc
     1321 aacctggcgg cggcgtacgg gattgcggtg accggaacca tggtactgac gtctattctc
     1381 tcgactaccg tggcacgtca gaactggcac tggaataagt attttgttgc gctgatcctg
     1441 attgctttcc tttgtgtcga tattccattg ttcaccgcta acctcgataa actgctctcc
     1501 ggcggctggt tgccattgag cctcggtact gtgatgttta tcgtgatgac cacctggaag
     1561 agcgagcgtt tccgcttgct gcggcggatg catgaacatg gtaactctct ggaagcgatg
     1621 attgcttcgc tggagaaatc accgcctgtt cgcgtgcccg ggaccgcggt gtatatgtcg
     1681 cgtgcaatca acgtcattcc ctttgcgctg atgcataacc ttaaacataa caaggtattg
     1741 catgagcggg tgattttgtt aactctgcgc accgaagacg cgccatatgt ccataacgtc
     1801 cgtcgggtac agattgaaca actgtcgccc actttctggc gcgtggtggc aagttatggt
     1861 tggcgagaaa cgccaaacgt ggaagaagtt ttccaccgct gcggtctgga aggattaagt
     1921 tgccggatga tggaaacctc cttctttatg tcgcatgagt cgttgatcct cggcaaacgc
     1981 ccgtggtatt tgcgtctgcg cggcaagctg tacttgctgc tgcaacgtaa tgcgctgcgt
     2041 gcaccagatc aatttgaaat cccgccaaac agggttatcg aactgggtac tcaggtcgaa
     2101 atctaacgcc agacgcctcc tttcttcata agggggcgtt tttgttttca tggttaatca
     2161 ccatgtaaaa cgtttcgagg ttgatcacat ttccgtaacg tcacgatggt tttcccaact
     2221 cagtcaggat taagctgcgg atcagcgaaa cgtttcgctg atggagaaaa aaatgaaaaa
     2281 aggcaccgtt cttaattctg atatttcatc ggtgatctcc cgtctgggac ataccgatac
     2341 gctggtggtg tgtgatgctg gtttacccat ccccaaaagt acaacgcgta tcgatatggc
     2401 attaacccaa ggtgtacctt cttttatgca ggtgctgggc gtcgtcacaa atgaaatgca
     2461 ggtcgaggct gccattatcg cggaagagat caaacaacat aatccgcaac tccacgaaac
     2521 gttgctcact caccttgagc agctgcaaaa acaccaggga aataccattg aaattcgtta
     2581 caccacgcat gaacaattca aacaacaaac cgcagaaagt caggcggtaa ttcgcagcgg
     2641 agaatgttct ccgtatgcga atatcattct ctgtgctggc gtgacgttct gaggccgtca
     2701 tggaagcatt acttcagctt aaaggcatcg ataaagcctt cccgggtgta aaagccctct
     2761 cgggcgcagc gttaaatgtc tatccgggcc gtgtgatggc gctggtgggc gaaaacggcg
     2821 cgggtaaatc caccatgatg aaagtgctta ctggtatcta tactcgcgat gccggcacgc
     2881 ttttatggct ggggaaagaa acgacattta ccgggccgaa atcttcccag gaagccggga
     2941 ttgggattat ccatcaggag ctgaacctga tcccgcagtt gaccattgcc gaaaacattt
     3001 tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga ctggaaaacc atgtatgt
//
LOCUS       DADWTH010000231         3038 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.237, whole genome shotgun sequence.
ACCESSION   DADWTH010000231 DADWTH010000000
VERSION     DADWTH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.237"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            53..1084
                     /gene="ynaI"
                     /locus_tag="JNY02_004188"
     CDS             53..1084
                     /gene="ynaI"
                     /locus_tag="JNY02_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBB9435457.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRERKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1135..2748)
                     /gene="mppA"
                     /locus_tag="JNY02_004189"
     CDS             complement(1135..2748)
                     /gene="mppA"
                     /locus_tag="JNY02_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBB9435458.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKGRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          790 a          707 c          672 g          869 t
ORIGIN      
        1 cgccaggggt ttccggcatg acgacaatta tctggaggag cctgataaaa caatgatcgc
       61 tgaactgttt acaaataatg cgcttaatct ggtcattatt ttcggtagct gcgcagcatt
      121 gattctgatg agcttttggt ttcgccgtgg aaatcgtgaa agaaaaggat ttttattcca
      181 tgcggtgcaa tttttaatct acaccataat tatcagtgct gttggtagca tcattaatta
      241 tgttatagaa aactataaac tcaaatttat caccccaggc gttatcgatt ttatctgtac
      301 gtccctgatt gcggttattc tgacgattaa gctatttctg ctgattaatc agtttgaaaa
      361 acagcagatt aaaaaaggtc gcgatatcac cagtgcgcgg attatgtcgc gcattatcaa
      421 aatcaccatt attgtagtgc ttgttctgct ttatggcgaa catttcggca tgagcctttc
      481 tggcttgctg acctttggtg gtattggtgg tctggctgtc ggtatggccg gtaaagatat
      541 tctgagtaat ttcttttccg ggatcatgct ctatttcgac cgtcctttca gtattggcga
      601 ctggatccgt tcaccggaca gaaatatcga aggtacagta gcggaaattg gctggcgaat
      661 taccaaaatt acgacctttg ataatcgtcc attgtacgta ccgaactcgc tgttttcgtc
      721 gatcagcgta gaaaacccag gacgaatgac caaccgccgc attaccacga ccattggttt
      781 acgttatgag gatgcggcaa aagtgggcgt tattgtcgaa gccgtacgtg agatgctgaa
      841 aaatcacccg gccatcgacc agcgacaaac cttactggtt tatttcaacc agtttgctga
      901 ctcgtcattg aatattatgg tttattgctt taccaaaacc acggtatggg ctgagtggct
      961 tgctgcacag caagacgttt atttgaagat tatcgatatt gtacagtcac acggcgcgga
     1021 ctttgccttc cccagccaga cgctgtatat ggataacatt acaccgccgg aacagggtcg
     1081 ctaaatcaga catcactggc gttatccaca agggtaacgc cagttcccat cacatcaatg
     1141 cttcacaata tacatagtcc gactgtacgc tacatcttca gggttattaa tgggataacc
     1201 tttcagccac ggtttgatta atcgtccatt ggtatattga taaattggtg caatcggtgc
     1261 ttgctccatg aggatttttt ctgccgcgtt gtaatcggca ttacgcgctt taacggtatt
     1321 ttccgtcgat gcctgggcca gaactttgtc gtatgccgga ttgttaaagc gtgaaatatt
     1381 tcctgaatgc attgacgtta ataatgtcag gaaagtggag ggttcattat aatcccccac
     1441 ccatgaggcg cggataacat caaaattgcc ggtgttacgg ctatcgatat aggttttcca
     1501 ttcctgattt tgcaatttaa catctacgcc aaggttcttt ttccacatcg atgctacagc
     1561 aatcgcaatt ttttgatggt tttctgaagt gttatacaaa agcgtcagct tcagcggttt
     1621 ttgcggacca taaccagctg cgctcaacaa agtttttgcc tgcgcattca gttcttcctg
     1681 actcatttgt tcaaacggcg aaggttccgg cgtaaatccg gcggtaacat ctggtataaa
     1741 atgccacgct ggcttttcgc ccgtccccaa cactttttcg gtcatcaggc ggcgatctat
     1801 cgtcatactt aatgccaggc gaacgcgctg atctgccgtc ggcccttttt gcgtgttaaa
     1861 ggcataataa taggtcccga gctgcggtgg cgtataaacc tgccccggaa tatccttcaa
     1921 cagcttctga tacatatttt tcgggaagga ttcggtgata tcaatatccc ccgcgaggta
     1981 acgcttagtg gctgcggatt cctgattaat tggcaggaag gtcacttttt gcagcaccgt
     2041 tttggcgtta tcccaataat gggtattcgg taccacgacc agtttttcat tgactacgcg
     2101 ccctttaaga acataagcgc cattgccgat cagatttccg ggtttcgtcc actctttacc
     2161 gctttctacg ttggcttttt gcaccgggaa gaaggcaaag ttagcggtta aattcacaaa
     2221 ccacggcaac ggtttatcaa gctgaatttt caaagtatgg gcatcaactg cggtgacgcc
     2281 aagctgatca ggcgtagctt taccatcaat aatcgcctgt gcgttgttga ttcccgccag
     2341 cgcggcaaac catgcaaatg gcgacaatgt ttttgggtcc accagacgtt gccagctgta
     2401 gacaaaatct tgcgccgtta ccggtgtgcc atccgcccat tttgcgttat cgcgcagggt
     2461 aaaagtccag atacggttgt cattactttt ccattgagtc gcgacgccgg ggacaatctc
     2521 ccctttttcg ttctgattca ccagaccttc aaacagatcg cgaatgacct gaatctctgg
     2581 caggcccacg gctttagcgg gatccagcga cgcaggctca tctttaatgt ggcgcaccag
     2641 ctcctgcttc tctgccagta ctgtgccgct cggaacttct gcagcatacg aaagagaaat
     2701 gctgctgacc aacagcgcac aacacgtaac tgaaacagag tgcttcataa gtttccctct
     2761 aaccgacatt aaaattgatg cgtaattatt tgtttcgcct taaagaaatg caaacaactt
     2821 ccatcaaaaa agtgacaaag gaagcatcaa atgttttaag tatcaatata tcaaatcaga
     2881 tttagtgtaa ataatagacg ccaaaatgcg gcaaagcaca tagaacaccg aacactacgt
     2941 tcgggcttcg cacgttcaaa ccagttttcg gtatcataat cagcatctgt cgccattgta
     3001 cgaagaataa aaaataacca aaacgttgaa aggaactg
//
LOCUS       DADWTH010000232         2992 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.238, whole genome shotgun sequence.
ACCESSION   DADWTH010000232 DADWTH010000000
VERSION     DADWTH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.238"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            43..357
                     /locus_tag="JNY02_004190"
     CDS             43..357
                     /locus_tag="JNY02_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435459.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            639..1205
                     /gene="ipgB2"
                     /locus_tag="JNY02_004191"
     CDS             639..1205
                     /gene="ipgB2"
                     /locus_tag="JNY02_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HBB9435460.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1467..1589)
                     /locus_tag="JNY02_004192"
                     /pseudo
     CDS             complement(1467..1589)
                     /locus_tag="JNY02_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1732..2286
                     /locus_tag="JNY02_004193"
                     /pseudo
     CDS             1732..2286
                     /locus_tag="JNY02_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2626..2992
                     /locus_tag="JNY02_004194"
                     /pseudo
     CDS             2626..2992
                     /locus_tag="JNY02_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          849 a          648 c          681 g          814 t
ORIGIN      
        1 ttatagaagc tgtcagcggc tacgtcccag ccaaatcact tcatgctcca gcgccagaga
       61 ggcaaggttg ggtttgctca gctgccggtt gattttgttg cgattgaacc ggacagcgtc
      121 cagggcgtcg gcaaaagagc gaatctcacc aatcgttgtt tcattatccg cattaacgat
      181 agcttcaaga aacgccaagg ttttatcgaa ttcatcagca acagtggctc cgggcatacc
      241 gtgaccgttt atacaaaacg gcgttttcag cgtggagttt tcatgaatag cctcaacacg
      301 aatgtcgtga agccagtgat gtatcggcta cgatcctcag ctgatgccag accataggac
      361 tgacactacg aacagcaatt ttaatgacgt aaatcttcat aactacaact aacagggcat
      421 cattatgaat gatgccggtt attttaccgc tgttggtccc ttcatttagg gactgcccca
      481 agttttttca tactctcttt ctcagagagt ttagtctctg ggattccaga cggtccatca
      541 ttgagccaag ggagtaaaga actcaaagca tttacagaat tgtttgaatc agctaaaata
      601 atgacaatgt tactatttta catgacaaag tgattataat gcttggaaca tcttttaata
      661 attttggaat cagtttatct cacaaaagat atttttcagg aaaggtggat gagattatcc
      721 gctgtacaat ggggaaaagg atagtaaaaa tatcatcaac caaaataaat actagtattt
      781 tgagctctgt ttcagaacaa atcggtgaaa atattacaga ttggaaaaat gatgaaaaaa
      841 aagtctacgt atcccgggtg gtgaaccaat gtattgataa gttttgtgca gaacacagcc
      901 gaaaaatcgg cgataatctc cgtaaacaga tatttaaaca ggttgagaaa gattatcgca
      961 tttctctaga catcaatgct gcacaaagtt ccataaatca tcttgtatca ggaagcagtt
     1021 atttcaaaaa gaaaatggac gaactttgtg aagggatgaa tagaagcgtt aaaaatgata
     1081 caacaagcaa tgttgcaaat cttatttccg atcagttttt tgagaaaaat gtgcaatata
     1141 ttgatctaaa gaaattacgt gggaatatga gtgactatat tacaaattta gaatcgcctt
     1201 tctgatgaaa gaacatcccg agtaaggaaa ctaagaaaag taaataccca ggaattgcct
     1261 gagcggtgct atatttctcc tcgcaagtaa gtcctaatat attgaattgt tgtatagcca
     1321 gtttttgtat tttcctagct catggatgtg ggatccctct cccctttctc tgctcaaagg
     1381 taagcggctc gtcagaatca tattgatatt tactgagaag tcagatcaac tttccaaggc
     1441 aacagatcgc gtacccggtt tgccggtcag tcctggatat gttcaataac gtaacgcagc
     1501 catttttctg gctccacatt gttcagacgg catgtgccga tcagcgagaa caacaccgct
     1561 gcatgttcgt caccgctgtc ggaacctgcg agtcgagtca gccggagaga gtttcactct
     1621 ccggctgctt acggaaccgt acgtgacact ctcgcgtcat acggctcttg atcactcatt
     1681 cgatgcacgt agtaaatacc agtgagcaaa ttgatcttac ccagcaatac ggtcagttac
     1741 cgcgcacatg gcctgcctgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
     1801 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagg ctggctgtat
     1861 ctggctgtgg tcattgacct gtggtcacgt gccgttattg gctggtccat gtcaccacgc
     1921 ctgacggcgc aactggcctg cgatgccctg cagatggcgc tgtggcggcg taagagaccc
     1981 cggaacgtta tcgttcactc ggaccgagga agtcagtact gttcagcaga ttatcaggcg
     2041 ctgctgaagt ggcataatct gcgtggcagt atgagcgcaa agggctgctg ctacgataat
     2101 gcctgcgtgg aaagcttctt tcattcgctg aaagtggaat gtctccacgg agaacacttt
     2161 atcagccggg aaataatgcg ggcaacagtg tttaattata tcgaatgtga ttacaatcgc
     2221 tggcgacggc acagttggtg tggcggcctc agtccggaac aatttgaaaa ccagaacctc
     2281 gcttaggcct gtgtccatat tacgtgggta ggatcactta cgccctgtgg ttgaaacgtg
     2341 agcatattgt tttcagatgc tgttgtcttg cttcagagtg atctgtcctc cggatttacg
     2401 caaagttatc cacaggcact gtgcatttaa aaaacttatc ttttcttctt gattttaaaa
     2461 actcagatcg tcatatcatt atgataaaca taataaaaaa ctctaaacat gagaaggttt
     2521 ttaacgtaag gtgagaggat ttttaagtgt atccatagat taaaaatgac aatgcctcca
     2581 attagttgaa ttaggggtta taatacccgc ttttaaggtt ctttcatggt cacagttaac
     2641 tttcattgcc cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaagga
     2701 catgatcgat tccgttgccg tgactgtcac cgggtgtttc agctcactta ctgttacgag
     2761 gcccgtaaac cgggagttaa aaaccaaatc actgaaatgg ctttcaacgg tgccggagtt
     2821 cgcgatactg cccgtacgct gaaaatcggc atcaataccg tcatacggac attaaaaagc
     2881 tcccgccccg gcggataaca tctgcgccag tcgctcacgc tgatgttgcc cttatctgtg
     2941 aacttgatgg gcagtggagc ttcgttggca gcaaggcgca acaacactgt ca
//
LOCUS       DADWTH010000233         2789 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.239, whole genome shotgun sequence.
ACCESSION   DADWTH010000233 DADWTH010000000
VERSION     DADWTH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.239"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..985
                     /gene="yeeE"
                     /locus_tag="JNY02_004195"
     CDS             <1..985
                     /gene="yeeE"
                     /locus_tag="JNY02_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBB9435461.1"
                     /translation="LTGGFRDMYIAKNNRMFYALLIAISAQSVGVFALIQAGLLTYEA
                     GAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSAVMRSPH
                     ASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMKELKKPKLKVATLPPR
                     RTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGVTSPTANILQFLVAG
                     DVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGG
                     CSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            999..1226
                     /locus_tag="JNY02_004196"
     CDS             999..1226
                     /locus_tag="JNY02_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBB9435462.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNY02_004197"
     CDS             complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNY02_004197"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBB9435463.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          643 a          660 c          742 g          744 t
ORIGIN      
        1 cctgactggt ggttttcgcg atatgtatat cgcgaaaaat aatcgcatgt tttacgcctt
       61 gctgattgca atttcggcac aaagcgtggg ggtttttgcg ttaattcagg cgggcctact
      121 gacttacgaa gccggggcgt tcccgtggct tggtactgtt ataggtgggt atcttttcgg
      181 gctgggaatt gttctggcgg gtggatgtgc caccgggacc tggtatcgcg cgggtgaggg
      241 attgatcggc agttggatcg cgcttttcac ttatatggtg atgagtgcgg tgatgcgttc
      301 tccacacgcc agtggtttaa atcaaacctt gcagcactac actactgaac ataactctat
      361 tgctgagact tttaatttgt ctgtgtggcc gttggttacc gttttgctgg tgataacgct
      421 ctgggtggtg atgaaagagt tgaagaagcc aaaactcaaa gtcgcgacct taccgccgcg
      481 ccgaaccggg atcgctcata ttctgtttga aaaacgctgg catccctttg tcactgctgt
      541 actcatcggt ttgattgcgc ttttagcctg gcccctgagt gaagcaaccg gacgcatgtt
      601 tgggttagga gtcacttccc caacggccaa tattctgcaa tttctggtcg cgggtgacgt
      661 gaaatacatt aactggggcg ttttcctggt gttagggatc ttcgtggggg catttattgc
      721 agccaaagcg agccgtgagt tccgcgttcg cgcagctgat gcacaaacaa cattacgtag
      781 cgggttaggt ggtgtactga tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg
      841 taatggactg gttatgactg caatgatgac ctggcagggc tggattggcc ttgtatttat
      901 gattctcgga gtctggactg cgtcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg
      961 actggcaact gctgcggcaa attaagtaag gtgtaaagat ggcgattaaa aagctggatg
     1021 tggtgacgca ggtctgtccg tttccactca tagaagcaaa agcagcactg gcagaaatgg
     1081 ccagcggcga tgagttggta attgagtttg attgtaccca ggcaactgaa gcaatcccac
     1141 agtgggcggc agaagaagga catgccatca ccgattatca gcagattggc gatgcagcgt
     1201 ggagcatcac cgttcaaaaa gcctgactca acattgtcct ccgccgtacc agcggcggcg
     1261 gcttcaaatt agacgatctc ttccgcgtac tgccaaagtg cttttaacag cgccactttc
     1321 tctttgtcat cggcatattg ttgtaccagc atctgcaatt catcagcata accctgcaaa
     1381 aactctggcg tgaagacctg gtggcggtgc tcctgccagc gttgctgctc ggcataatcc
     1441 agcgtccccg ggaagttgcg tgcccgataa ttgaacaaca acttttcaat tcgtttatca
     1501 acaaaagtga tatccagtgc cggtaaatta cgcggctcgg tttccagcac aattttcatt
     1561 gctgcacgat cggcgtcact gaaaaagcca ttataaagct gtgcatccac attatctgaa
     1621 ggtgtaaacg gttcggcttc cgcgaatatc gctaccactt tttcacgcac ttgcggattt
     1681 tcacgcagaa tttttaggtt atcgaggcaa tgctgacgat taattcccag tcggtcggca
     1741 tcttccgggc gtagcgtatt cgcctgggcc agtaccggac atttattgat atgcaccagc
     1801 ttaaccggaa cggcggcgtt atcgccaaga tcggcttttg cggtatataa acgctcgcgc
     1861 aatgtgtcga tatccagctc cagtaatggc gaaatgtctc ctgccaaatc cgccataatt
     1921 acggcattgc gattttcagg atgccacgcc agcggtgcca cccagctggt attgccacgc
     1981 catgccccaa acattccgga aacgtgagcc aggggtttca tctgcggaac atcaatcaac
     2041 gccatcagtt tgtgtttatt acgatgggta aagagataat caaacaggcg tggctgacgc
     2101 gtttttacca gtttcgccat cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg
     2161 ttgctatgtt caataccatt cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg
     2221 tcatcatttt caggccagtt tattccttcc gggcgcaggg cataacaggc acgcataaca
     2281 tccagtaaat cccagcgcga gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa
     2341 ttacgataaa aaacgttgcg tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga
     2401 atacaggtct tcggtacggt aaaaagcgag tgaatacggg cggcaaacgc ggcttcgttt
     2461 tctcctttcg cccgtgcctc ctgcggggta ataccggtaa ttaatactgc tccaggccgg
     2521 ggtaaatagt catccgcggg cttgcagtaa aagacttcgg gttcgccgat gacattgaat
     2581 tcgtcatcgg tgcgaatggc tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca
     2641 aaggtttcgt aatcgtgaaa gaggaaggta ggttgtttat ctgtgtctgt cattgtctag
     2701 cattgtccac tgtagttaat taactgtctg ttataactcg aaaagcaacc cttttttcac
     2761 ctgagtatgt ctacgaacgt caaatatag
//
LOCUS       DADWTH010000234         2732 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.240, whole genome shotgun sequence.
ACCESSION   DADWTH010000234 DADWTH010000000
VERSION     DADWTH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.240"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..546)
                     /locus_tag="JNY02_004198"
     CDS             complement(<1..546)
                     /locus_tag="JNY02_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435464.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2194)
                     /locus_tag="JNY02_004199"
     CDS             complement(1208..2194)
                     /locus_tag="JNY02_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435465.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTESSFGSEVTDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGADRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
     gene            complement(2276..>2732)
                     /locus_tag="JNY02_004200"
     CDS             complement(2276..>2732)
                     /locus_tag="JNY02_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435466.1"
                     /translation="TDINLYENTVSLDGQSYPLSLAIKTLIPGYLSGLPSTSRESMDL
                     LEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKERTREMFMTEDEW
                     QKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          723 a          629 c          671 g          709 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagatta atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgcaccc acgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga tgtctgtacc cagcatatcg acaatagcgt
     1501 cttcgccacc gaagccctgc ttagcagcca gacgggaaag agccttgagg tgtgactcat
     1561 gggcgatgat accgtaatca gtaacctcag aaccgaaaga agactcggtg ttctcataca
     1621 gagcgtccat gatagtgtct gccttagcgg tcaggctacc agtcaggacc ggaagctcta
     1681 cggtcttcag cagcttagag gactccagaa cgtcaaccat cttagagtta atggtacgga
     1741 ccacgttatc gcagatgttc agagcatcct gctggagcgt cccccgagtg aaaccagtag
     1801 aggacttctg gtcccagagc tttgtctgat gagacacgag gatagcctgt tcaatcggct
     1861 caacggtgat ttcagtacca gacggagcgg tcacgacttc aggtcgctta ccaccacgga
     1921 caggaacgac cacagagcgg tcagccagag tgtgagcaat gattggcgtc ggtaagctgt
     1981 tacgggtcgg caggacttct acagggaacg gaatgttttc ctgagcgtct ttaagtacac
     2041 cgttcagcat agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt
     2101 tagtaggcag accttcattg acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa
     2161 taccgaactg gaaagcgtct ttaatcgtca taataatcaa tctcctaaat tagtttaaat
     2221 actgtgagtc ctcaaaactg aggagtccct agcatcgggt cagggactgt gtgttttaca
     2281 ggctgtccat ccagttaccg cctgactcaa ggtcagcgac gatagcggca cgggagcggc
     2341 cagcggaatg ggtagcactg cggaggtttt caccataggt ccggcgttca gctttgacct
     2401 gttcgcggcg ctgtagctct ttttgccatt cgtcttcggt catgaacatt tcgcgggtac
     2461 gctctttctg agacgctagc tctttagcga tacgctcagc ttcagctcgt ttgttcatct
     2521 ggcggcgttg gtaggcggtc atcaggtcat tactgaagaa gttaccaatg gtcacgccag
     2581 cttcagccag agcttccagt aaatccatag actcacggct ggtagacggc aggccggaca
     2641 ggtagcccgg aatgagggtc ttgatagcga gcgacaacgg ataggactga ccgtccagac
     2701 tcacagtgtt ttcataaagg ttgatgtcgg tg
//
LOCUS       DADWTH010000235         2648 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.241, whole genome shotgun sequence.
ACCESSION   DADWTH010000235 DADWTH010000000
VERSION     DADWTH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.241"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1475
                     /locus_tag="JNY02_004201"
     CDS             <1..1475
                     /locus_tag="JNY02_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBB9435467.1"
                     /translation="AIGLSIAAGGAALTWYQVAETDLSRTAAAAHEAVSVTERLLDEA
                     QGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLSCSSWEGADPVREF
                     ISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVADNMTLLNLRRPLS
                     LRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPLTLYFHDGALSLLL
                     SVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDASTGEIAGVEVLAR
                     WVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLPPLTGDGKRWHISFNITQS
                     HIMETGFIAECLTFIDAFTPGSITLTIEPTEREPFDNSDEMRCQLKRIHDAGIALALD
                     DFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIEMANTLKKEIIAEG
                     VETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT          682 a          558 c          618 g          790 t
ORIGIN      
        1 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
       61 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
      121 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
      181 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
      241 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
      301 gggagttcat ctccgctggc gacaaccaga cgctgattct ggcgacagac gattatatat
      361 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
      421 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
      481 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
      541 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
      601 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
      661 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
      721 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
      781 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
      841 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
      901 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttaccg cctctgacag
      961 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
     1021 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
     1081 ctatagagcc cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
     1141 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
     1201 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
     1261 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
     1321 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
     1381 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
     1441 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
     1501 cattgaacct gtgagggccg ccccctttat tactcagggg ggctttcaac gttctgaatt
     1561 aagatgtttt atgactcact gagcaggatg tgagttccaa cgtaatctcc gctattttta
     1621 ctgtttttca tttgctgcct catctatccc ttcgccttgc ctctgagttg cgttaaaggc
     1681 tgatgtactg cctttaaccc gttcaataca tagccttttt gtcaatcatc atcccgaaga
     1741 gcgttgcact gtgccttcca tagctgtggt tgtcctctca atctgtcagc ccatttatgg
     1801 aaaatcttaa cgaagcaaca atgagatatg taaaaaaagg gccgatttaa tcattcatta
     1861 agacaggtca ttttttctta ctgtgagggc ggtaagcttt tctgtcggta acaggttggg
     1921 agatgctggt attatgagat ttttctaccg tcctgcggcg gaatatgtga aaaatagaaa
     1981 tgattattat tattgcatgg ttatttttag tttgtgatgg tggagaataa tcctattccc
     2041 gtgaccttgt tttttacatt ttatttaact gcatggtata tgcattttgg acggtgaaag
     2101 caagaagcta ttaggcgcta taggtctttt gacggttttt gaatttcccc tcctgatatc
     2161 tctgttatat ttttgtgaat ttctccaatc caaatcaaag tcccaataag tcatggatgc
     2221 atggttaaaa taactatcat ttgtgtaacc tttggtgctt ataacttttc tttattcttt
     2281 tttcaggatt acctggaggg attgaccaaa aaaacattca ttgaatgata tttttctaat
     2341 taatgaggtt ttctaagctc gttgaaataa aaaatatatt ttaatcatgg atttgcatgc
     2401 gtttctttcg ctttttttac acaagttgac attgaactca gcaactataa gaatagccac
     2461 atcccagacg gtgaccgata aaggaaatac cgttaccata tttaaatgaa ttggaaaata
     2521 tcaccgattt gaagtcgact cgttcgggta attattaata ctgaatcttt aatgcttatt
     2581 tcttaagctt gttaacaacg ggaatgaata atgaaagtgt ccaaaattgc actggcaatg
     2641 ggtcttag
//
LOCUS       DADWTH010000236         2624 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.242, whole genome shotgun sequence.
ACCESSION   DADWTH010000236 DADWTH010000000
VERSION     DADWTH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.242"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..106)
                     /locus_tag="JNY02_004202"
     CDS             complement(<1..106)
                     /locus_tag="JNY02_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBB9435468.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="JNY02_004203"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="JNY02_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBB9435469.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="JNY02_004204"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="JNY02_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBB9435470.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="JNY02_004205"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="JNY02_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBB9435471.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2425..>2624
                     /locus_tag="JNY02_004206"
     CDS             2425..>2624
                     /locus_tag="JNY02_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HBB9435472.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSA"
BASE COUNT          768 a          553 c          498 g          805 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctca tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatgcctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagc
//
LOCUS       DADWTH010000237         2504 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.243, whole genome shotgun sequence.
ACCESSION   DADWTH010000237 DADWTH010000000
VERSION     DADWTH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.243"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            156..1109
                     /locus_tag="JNY02_004207"
                     /pseudo
     CDS             156..1109
                     /locus_tag="JNY02_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1150..2145)
                     /gene="pgl"
                     /locus_tag="JNY02_004208"
     CDS             complement(1150..2145)
                     /gene="pgl"
                     /locus_tag="JNY02_004208"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBB9435473.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYSVGQGPMWVVVNAH"
BASE COUNT          692 a          613 c          561 g          638 t
ORIGIN      
        1 ttattgcgat atggttacat taaggacaaa gcatctctaa tgactttagc tcaacgatta
       61 ataacatttt ttcattaatt taagttggct aaatgcagtt aaaattttta acgaccagcc
      121 ccccaaaatc gtcgcttcca ttttccggag taataatgaa gcatgaatta tcaagtatga
      181 aggcatttgt catactggca gagtccagtt catttaacaa tgccgctaaa ttactcaata
      241 ttacgcaacc tgcattaacg cgaagaataa aaaagatgga ggaagattta catattcaac
      301 tatttgagcg tacaactcgc aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg
      361 aggcgcggga attaataaag aaattcgatg aaacgctttt taatattcgt gatatgaatg
      421 cttatcatcg tggtatggtg acattagcgt gtattccgac cgcagtattt tattttttac
      481 cgctggcaat cggtaaattt aatgagctgt atcccaatat taaagtgcgg attctggaac
      541 aaggcacgaa taattgcatg gaatcggtgc tgtgtaacga atcagacttt ggcataaaca
      601 tgaacaacgt cacaaattca tccattgatt ttaccccact ggtcaacgag ccattcgtgc
      661 tggcctgtcg gcgtgaccat ccattggcca aaaagtagct tgtagaatgg caggagttag
      721 tgggttacaa aatgattggc gtacgttcct ccagcggcaa ccgattgttg atagaacaac
      781 agctagccga caagccctgg aagctggact ggttttacga agtgcgtcat ctttcgacgt
      841 cattaggact agtggaggca ggactgggga tttcggcgct tcctggtctg gcaatgcccc
      901 atgcgcccta ttcttcgatt attggtatcc cactggtgga acccgttata cgtcggacat
      961 tggggattat tcgccgcaaa gatgccgtgc tttctccggc agctgaacgc ttttttgcct
     1021 tgctgattaa tttgtggact gacgataaag acaatttgtg gaccaatatc gtcgaacgcc
     1081 agagacatgc gttacaggag ataagttaat acaccggcgc aggagagact gcgccggtta
     1141 aatcagcggt tagtgtgcgt taaccaccac ccacattggt ccctgcccga ccgaatagcg
     1201 gcctttttca tgcagtagcc cctgctcgcc aacaatttcg tataccgaga tgtggtgaga
     1261 tttttgccca gcggcaatca gatacttgcc gctgtaatca acattgaagc cgcgcggctg
     1321 ggtttccgtt ggctggaagc cttctttact caacacgctg ccatcttccg aaacgctgaa
     1381 aacggtaatc agactggcgg tacggtcgca ggcgtataaa tggcgaccat ccggggtgat
     1441 atgaatatca gccgcccaac gggtgtcgga gaagttttcc ggcatcatat ccagcgtctg
     1501 gacacattcg atattgccgt gcggatcttt cagttcccag acatccactg agctgtttaa
     1561 ctcattgacg caataagcat attgttcgtt tggatggaat accatatgac gcgggccggc
     1621 cccttcaacg gtagtcactt ccgcagggtc ctgcgccacc agatgaccat catcgctgac
     1681 cgtaaacagg caaatgcgat cttgctttaa tgccggaacc cacagcgtac ggttgtccgg
     1741 tgagatattg gcggaatggc aaccgtccag cccctcgact acatcgacga cgcccactgg
     1801 caggccatct tccagacgcg ttacgctcac gttacccgca ttgtaagaac ctacaaagac
     1861 aaactgcccc tggtgatcgg tggaaatatg cgtcggacta cccggcagca cagactctgc
     1921 ggcaaaggtc agtgcgccat cgtccggggc aatacgatac gccaggacgc gaaactcagg
     1981 gcgaacacca acatagagat aacgtttgtc cgggctgacc accatcggct gcacctgccc
     2041 cggcacatcg acaacctgtg tcagcgtcag tgcgccttca tgattcaggt tccagacgtg
     2101 aatttgctgg ctctcagggc tggcgatata aactgtttgc ttcatgaaca cacctcaaaa
     2161 atgataaatc ccttatcttt catacatata acaacactta ttggataaaa aacagacaac
     2221 aaaacacgat aaaatacaat atcgcacatc aattaaatca ataagttagc actccatttc
     2281 gaatggcata ttactaaaat attagtcacc gacacgaatt tgacaatgcc gcagatattt
     2341 tcgcgactat tcagggagta tgactctccc cgcttttgtt tacctgtatc aacgaccgta
     2401 aacactatgc cataacaact tctggccaac ttcccattcg ctcaacaatc aactcacttc
     2461 cggcacaaat aaacgcatta cgttgtagat cggtattctg gtca
//
LOCUS       DADWTH010000238         2477 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.244, whole genome shotgun sequence.
ACCESSION   DADWTH010000238 DADWTH010000000
VERSION     DADWTH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.244"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            33..1085
                     /locus_tag="JNY02_004209"
     CDS             33..1085
                     /locus_tag="JNY02_004209"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBB9435474.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMCLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1161..>2477
                     /locus_tag="JNY02_004210"
     CDS             1161..>2477
                     /locus_tag="JNY02_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBB9435475.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSTA
                     FVTTGLGKRIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPDCRIAGLPDCRIAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAG
                     LAELGPMKIREKMLLGVFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVV
                     KNKGGWNTLIWYGGIIGLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSII
                     VRYFFASGSAYIVAMLPVFAMLANVSGAPLMLTALALLFSNSYGGMV"
BASE COUNT          588 a          556 c          639 g          694 t
ORIGIN      
        1 ataagttatg tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc
       61 gaggtggaac ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc
      121 agcgcgataa aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta
      181 ttggcggcgg taatccactg accagtaaag tcgccattat tagccgttcc agcgatccgc
      241 gtgctgatgt cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca
      301 cgcctaactg cggcaatatg ctgtctggcg taggggcatt cgccattgaa aatggtttga
      361 ttgcagcgac ttcgccagtt acccgtgtac gtatccgcaa cgtcaatacg ggtacgttca
      421 tcgaagctga tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg
      481 acggcgtacc gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gctggaacga
      541 aaaccggaaa agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga
      601 cctgtatcga tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag
      661 gttatgaatt accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta
      721 tccggctaca agcgggtaaa gcaatgtgct taggtgatgt cagtaatatg gttatcccta
      781 aacctgtgct tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc
      841 cgcattcttg ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg
      901 cattggaagg caccgtcacc cgacaaatcg tcccttctgt aggatatggc aatatcaata
      961 ttgaacaccc cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga
     1021 cgttacgcgc atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttatcttc
     1081 cctgaaaaaa ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata
     1141 cagaaagaac aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag
     1201 cgatcccatg tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg
     1261 tgctttttgg tatttacctg gcggccattg tgggtctggt tatcaagcct ttcccggaac
     1321 ctgtcgtact gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg
     1381 gtgcgtttaa gaccaccgcc gtattaagcg gttactcttc aggtactacc tggctggtgt
     1441 tctcggcgtt taccttaagc accgcatttg tgaccaccgg tttaggtaaa cgtattgcct
     1501 atctgctgat tggtaaaatc ggtaacacca tgctgggtct gggttacgtt acggtattcc
     1561 tcgatctggt actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt
     1621 taccgatcat caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc
     1681 gtgtcggaca ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt
     1741 tctttaccgc aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc
     1801 tgcaaattag ctggggtgga tgggcgctgg ccgccggatt gccggattgc cggattgccg
     1861 gattgccgga ttgccggatt gccggattgc cgggcatcat tatgctgctg gtcaccccgc
     1921 tggtaattta caccatgtat ccaccagaaa ttaaaaaggt ggataacaaa accatcgcta
     1981 aagcgggcct tgccgaactg ggaccgatga aaatccgcga aaaaatgctg ctcggtgtct
     2041 ttgtgctggc gctactgggc tggattttca gtaagtctct gggggttgat gaatccactg
     2101 tggcaatcgt tgttatggcg accatgctgc tgctgggtat cgttacctgg gaagacgtgg
     2161 ttaaaaataa aggcggctgg aataccttaa tctggtacgg cggtattatc ggcttaagct
     2221 ccttattatc gaaagttaaa tttttcgaat ggttagctga agtctttaaa aataacctgg
     2281 catttgatgg tcacggtaac gttgctttct tcgttattat tttcctcagc atcatcgtgc
     2341 gttatttctt cgcttccggt agtgcctata tcgttgctat gttacctgta tttgccatgc
     2401 tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct ggcactgttg ttctccaact
     2461 cctatggcgg catggtt
//
LOCUS       DADWTH010000239         2456 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.245, whole genome shotgun sequence.
ACCESSION   DADWTH010000239 DADWTH010000000
VERSION     DADWTH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.245"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            9..2243
                     /gene="xdhA"
                     /locus_tag="JNY02_004211"
                     /pseudo
     CDS             9..2243
                     /gene="xdhA"
                     /locus_tag="JNY02_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004089117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            2254..>2456
                     /locus_tag="JNY02_004212"
     CDS             2254..>2456
                     /locus_tag="JNY02_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBB9435476.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
BASE COUNT          593 a          649 c          647 g          567 t
ORIGIN      
        1 aatcatgcat gcgcgtcgat tccattgcta aggtcaccgg gcgggcacga tatactgacg
       61 attatgttat ggcgggcatg tgttacgcga aatatgtacg tagccctatc gcacatggtt
      121 atgccgtaag tattaatgat gaacaagcca gaagtttacc gggcgtactg gcgattttta
      181 cttgggaaga tgtgcctgat attccattcg ctacagctgg gcatgcctgg acacttgacg
      241 aaaacaagcg cgataccgcc gatcgcgcac tgctaactcg ccatgttcgt catcatggtg
      301 acgccgttgc catcgtcgtg gcccgcgatg aactcacggc agaaaaagcg gcgcaattgg
      361 tcagcattga gtggcaagaa ttacccgtta tcaccacgcc agaagcggcg ctgacagaag
      421 acgctgcacc aatccataac ggcggcaatt tactgaaaca aagcacgatg tcgacgggca
      481 atgtccaaca aacaatcgat gccgccgact accaggtaca ggggcactat cagactcccg
      541 ttattcaaca ttgtcacatg gaaagcgtaa catcgctggc gtggatggag gatgactcgc
      601 gaattaccat cgtttccagc acccagatcc cgcacattgt tcgccgcgtg gttggtcagg
      661 cgctggatat tccctggtca tgcgtacgag tcatcaaacc gtttgtcggt ggcggttttg
      721 gtaataaaca ggatgtactg gaagagccaa tggcggcatt ccggtgaaag tttcccttag
      781 ccgtgaagag tgtttcctcg caacccgtac ccgccacgct tttactattg acgggcaaat
      841 gggcgtgaac cgcgacggaa ccttgaaagg ttatagtctg gatgttctgt ctaacaccgg
      901 cgcttatgca tctcacgggc actccatcgc ttctacgggg ggggaataaa gtcgcttacc
      961 tttatcctcg ttgtgcctac gcttacagtt caaagacctg ctataccaac ctcccctcgg
     1021 ctggtgcgat gcgtggttat ggcgcgccac aagtcgtatt tgcctttgag tctatgcttg
     1081 atgacgccgc gacagcgtta ggtattgatc ctgttgaaat tcgtttacgc aacgccgccc
     1141 gcgaaggaga tgctaatccg ctcacgggca aacgtatttg cagcgcaggg ttgccggagt
     1201 gtcttgaaaa aggccggaaa atctttgaat gggaaaaacg ccgtgcagaa tgccagaacc
     1261 agcaaggcaa tttgcgccgc ggcgttggcg tcgcctgttt tagctacacc tctaacacct
     1321 ggcctgtcgg cgtagaaata gcaggcgcgc gccttctgat gaatcaggat ggaaccatca
     1381 acgtgcaaag cggcgcgacg gaaatcggtc agggtgccga caccgtcttc tcgcaaatgg
     1441 tggcagaaac cgtgggggtt ccggtcagcg acgttcgcgt tatttcaaca caagataccg
     1501 acgttacgcc gttcgatccc ggcgcatttg cctcacgcca gagctatgtt gccgcgcctg
     1561 cgctgcgcag tgcggcacta ttattaaaag agaaaatcat cgctcacgcc gcagtcatgc
     1621 tacatcagtc agcgatgaat ttgaccctga taaaaggcca tatcgtgctg gttgaacgac
     1681 cggaagagcc gttaatgtcg ttaaaagatt tggcgatgga cgctttctac caccctgaac
     1741 gcggcgggca gctctctgct gaaagctcca tcaaaaccac cactaaccca ccggcgtttg
     1801 gctgtacctt tgttgatctg acggtcgata ttgcgctgtg caaagtcacc atcaaccgca
     1861 tcctcaacgt tcatgattca gggcatattc ttaatccact gctggcagaa ggtcaggtac
     1921 acggcggaat gggaatgggc attggctggg cgctatttga agagatgatc atcgatgcta
     1981 aaagcggcgt ggtccgtaac cccaatctgc tggattacaa aatgccgacc atgccggatc
     2041 tgccacaact ggaaagcgcg ttcgtcgaaa tcaatgagcc gcaatccgca tacggacata
     2101 agtcactggg tgagccaccc ataattcctg ttgccgctgc tattcgtaac gcggtgaaga
     2161 tggctaccgg tgttgcaatc aatacactgc cgctgacgcc aaaacggtta tatgaagagt
     2221 tccatctggc aggattgatt tgaggataac atcatgtttg attttgcttc ttaccatcgc
     2281 gcagcaaccc ttgccgatgc catcaacctg ctggctgaca acccgcaggc caaactgctc
     2341 gccggtggca ctgacgtact gattcagctc caccatcaca atgaccgtta tcgccatatt
     2401 gttgatattc ataatctggc ggagctgcgg ggaattacgc tggcggaaga tggctc
//
LOCUS       DADWTH010000240         2437 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.246, whole genome shotgun sequence.
ACCESSION   DADWTH010000240 DADWTH010000000
VERSION     DADWTH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.246"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..360
                     /gene="capU"
                     /locus_tag="JNY02_004213"
     CDS             <1..360
                     /gene="capU"
                     /locus_tag="JNY02_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBB9435477.1"
                     /translation="DDVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVP
                     VVATQIGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEER
                     FDINKTALKILTLAKQK"
     gene            365..1315
                     /gene="virK"
                     /locus_tag="JNY02_004214"
     CDS             365..1315
                     /gene="virK"
                     /locus_tag="JNY02_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBB9435478.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1380..2324
                     /gene="lpxM"
                     /locus_tag="JNY02_004215"
                     /gene_synonym="msbB"
     CDS             1380..2324
                     /gene="lpxM"
                     /locus_tag="JNY02_004215"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBB9435479.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          707 a          470 c          549 g          711 t
ORIGIN      
        1 gatgatgttt ttattgctga caatgttttt cctgccgccc ccgtatatcg ggttgccagt
       61 ctggtggttc tgccttcaga aaacgaatct tttggtatgg tgctggcaga agcatcggca
      121 ttttctgtgc ctgtagtggc cactcagatt ggtggaatcc ctgaggttat tcagaacaac
      181 cagaccggga cattgttacc agcaagtaat aagcacgcat ggatgtgcgc cctgaatgat
      241 ttttttaatg accctgggcg tttttatcag atggctcgcc tggcaaaaca ggatatagaa
      301 gagcggtttg atattaataa aactgcgtta aaaatactca cattagcgaa gcaaaagtaa
      361 caatatgttt tctataagta acttatcatt tatcggtttc cttaaaagga ttattttttc
      421 ctcagattca ctaccgggga agtgggaaca cagaaaattt cggttcatgt acattttgcg
      481 atgttctata aatccggttg tcagtattcg atattattat gaactgcgtt ccttgccgtg
      541 cattgaggat attctggcaa tacaccccac gttgccagca agaattcatc gcccttattt
      601 gcataaaggg gggcgcgcct ggacccgtgg acaatatatt cttgagcatt atcgctttgt
      661 gcaaaatttg ccagaaaaat attctaaatt tttgtttcca caaaaatcgg tatcattagt
      721 ccaattcatc gggaaagatg gagagaattt tgatatccag tgttctccca gtggttttga
      781 ccgggaagga gagttgatgt tgtcattgtt ttacaataaa acagttattg cccggttaac
      841 attttcagtc attctgactc aaaatggaca cattgctttt attggcggat tacagggagc
      901 ccctaaaaat accggacctg atgttattcg gtgcgcaacc cgggcctgtt atgggatttt
      961 cccaaaacgg attatttttg aagcattttg tgcgttgatg aaagcttgca atatctctga
     1021 gtgtctggca gtcagtgagc acagtcatgt tttccggcaa ttgagatact ggtatcagaa
     1081 acgcaaaacc tttgttgcgg tctatagtga tttctgggag tctgtagcag gaaaaacctg
     1141 tggtgactgg tacagattac caacccaggt gattcgaaag ccgctaagcg atatagccag
     1201 taaaaaacgc tctgagtacc gaaaacgata cgctttgctg gattatatcc atgaaactac
     1261 tatccgctct ttggatgcat atcctgtaca ctcagaacat cagaatttaa attaaggttt
     1321 gatgtattct ctgaataaaa cagtaatgat aattttggta gaggcattcg cactaaataa
     1381 tgaaaaaata taaatctgag tttattcctg aatttaagaa aaattacctt tcccctgttt
     1441 actggtctac atggttcctt ttgggaatga ttgcaggtat ttcaatgttt cccccttcat
     1501 tcagagatcc tgtcttggca aaaatagggc gttgggcggg cagattgagc aaaaaagctc
     1561 gtcgcagggc gacgattaat ttatcgcttt gtttcccaga aaagagtgat acagaacggg
     1621 aaataattgt cgacaaaatg tttgtcacag cattgcaatc catagtgatg atggcagaac
     1681 tggcaattcg tggtccggaa aagttccaga aacgagtgtt ctggaaaggc ctcgaaattc
     1741 ttgaggaaat tcgacacaat aatagaaatg tgatttttct ggttccccat ggctggagcg
     1801 tggatattcc tgcaatgttg ctggcagctc agggaaaaaa aatggccgcc atgtttcatc
     1861 agcaacgaaa tccagtgatt gattatgtct ggaattcagt acggcgtaaa ttcggggggc
     1921 gcttacatgc ccgggaggat gggataaaac catttattca gtcagtacgc cagggatact
     1981 ggggatatta cctcccagat caggatcatg gtcctgaata cagtgaattt gctgattttt
     2041 ttgcgaccta taaagcgaca ttaccaatca ttggacgtct gatgaacatc agtcaggcta
     2101 tgattatacc acttttcccg gtttatgatg aaaaaaaaca tctactgact attgaaattc
     2161 ggccaccaat ggatgcttgc attgccagcg cggacaataa aacgattgcc cgacaaatga
     2221 acaaaacagt ggaaattttg gtggggccac atccggaaca gtatgtctgg gttttaaaat
     2281 tgttaaaaac acgcaaatca aacgaagcgg acccgtaccc ttgaaattat aaagtacaag
     2341 tatttccact agttgtttgt aagcgtacag cctgaaccgt ctggtcagaa tctgacgaat
     2401 tagacaaagt ggtgtccacc aaataagtag tgggaac
//
LOCUS       DADWTH010000241         2436 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.247, whole genome shotgun sequence.
ACCESSION   DADWTH010000241 DADWTH010000000
VERSION     DADWTH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.247"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1334
                     /locus_tag="JNY02_004216"
     CDS             <1..1334
                     /locus_tag="JNY02_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005029490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HBB9435480.1"
                     /translation="VFSITKKHQPYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRI
                     TRTTSGANAGGGNLTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIEE
                     IIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTE
                     TDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPNA
                     TEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAASRGEFVE
                     GISDPNDSKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQTGEDNCPDC
                     GAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELN
                     WKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            1312..1608
                     /locus_tag="JNY02_004217"
     CDS             1312..1608
                     /locus_tag="JNY02_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBB9435481.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            1614..>2436
                     /locus_tag="JNY02_004218"
     CDS             1614..>2436
                     /locus_tag="JNY02_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB9435482.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEK"
BASE COUNT          740 a          574 c          602 g          520 t
ORIGIN      
        1 cagttttctc tatcacgaaa aaacatcagc cctatgaatt taagaatttc atttcagcct
       61 ggctggatac cgaacacatt gatcgcggtc ttctgacaaa agaatggcga aaagggaatc
      121 gtgtttcacg catcactcgc acgacttccg gtgctaatgc tggcggcggg aacctcaccg
      181 atcgcggcga aggtttcgtc cacgatctga cgtcactgac gcgcgatgta gccactggcg
      241 tactggcccg ttcaatggac gtggacatct ataaccttca tccggcacac gctaaacgca
      301 ttgaggaaat tatcgctgaa aataaaccac ccttttctgt tttccgcgac aaattcatca
      361 ccatgcctgg cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg aaagaagcac
      421 caattgggat cgaggtcatc cccgcacacg tcactgaata tctgaacaaa gtactgactg
      481 aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg
      541 ccccgatgcc gcagcgagta acagaagaag gaaaacagga cgatgaagaa aaaccacaac
      601 catctggcgc aatgacagat gaacaggcaa cggctgaaac agtggaaccg aatgcaactg
      661 aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga
      721 aatatcagga actgcgggca gaactccatg aagcccggaa aaacattccg cccaaaaatc
      781 ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg
      841 acccgaatga ttcgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaaaa
      901 cggaaaatga agtgcgacag actgaagata aacagcacca gaacagcgaa ccggaggagg
      961 gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg gtgatgggag
     1021 acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag gaagaagagc
     1081 cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc gatcagcata
     1141 atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag gtgttgattg
     1201 cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct gcaatccctg
     1261 acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt gatgagcgct
     1321 tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca gcagatcgtt
     1381 cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc tcagcacctg
     1441 tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca ggcaatcagc
     1501 cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg
     1561 gctgaaacca ttgcccacca ccaggttgat attgatttgg aggactaaaa tctatggcgc
     1621 tttttcaacg agcaataaac acacaggcct atctaaaagc aggaattatg gggtttgctg
     1681 gcgacggcaa aacctacaca gcaagcgaac tggctattgg tctggtaatg cttatgcggc
     1741 aacggggaat tgaagctggt aaccgcccgg taatgtttct ggacacagaa acaggctctg
     1801 actgggttaa accccgcttt gatgcggaaa atatcgaatt atttacggca aaaacccgct
     1861 cgtttgttga tctgcttgaa gccattaatg aagcggaaag cagcggttcc gtaatgatca
     1921 tcgactccat cagtcacttc tggaccggat tatgcgacga gtatgccaga cgccgaaacc
     1981 gtaaacgagg actggaattt tcagactggg cgtggctcaa acaggagtgg cgacgcttta
     2041 ccgatcgatt tgttaacagt caggcgcaca ttattatgtg cgggcgtgca ggatatgagt
     2101 atgacttttt cgaaggtgat gatggcaaac gccagttggc aaaaaccggt ataaaaatga
     2161 aagcagaaac agaaaccggt tatgaaccct ccatcctgat ccagatggag aagcaaatga
     2221 acatagaaac cggtcaggtg tggcgcaccg ccagaatact aaaagatcgc tccacccgta
     2281 ttgatggaca ggtattttca aacccaacat ttaaaaactt tcttcctcat atcgagtttc
     2341 ttaacctggg aggtgaacac ctcggcatcg atacctcacg ggataacagt gaattatttg
     2401 ccaacgacgg tacaccgaca tggctaaaag aaaagc
//
LOCUS       DADWTH010000242         2373 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.248, whole genome shotgun sequence.
ACCESSION   DADWTH010000242 DADWTH010000000
VERSION     DADWTH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.248"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..823)
                     /locus_tag="JNY02_004219"
     CDS             complement(<1..823)
                     /locus_tag="JNY02_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBB9435483.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="JNY02_004220"
     CDS             complement(1424..1672)
                     /locus_tag="JNY02_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBB9435484.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="JNY02_004221"
     CDS             complement(2027..>2373)
                     /locus_tag="JNY02_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBB9435485.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          439 c          575 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DADWTH010000243         2355 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.249, whole genome shotgun sequence.
ACCESSION   DADWTH010000243 DADWTH010000000
VERSION     DADWTH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.249"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            746..2254
                     /gene="ltrA"
                     /locus_tag="JNY02_004222"
     CDS             746..2254
                     /gene="ltrA"
                     /locus_tag="JNY02_004222"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBB9435486.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYKF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          594 a          584 c          697 g          480 t
ORIGIN      
        1 cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc
       61 tgattttact tacggtgtgc ccggagttca gggcgggcat ggatgcttaa atgaaccgcg
      121 agtctgtctg gaatattgaa ccggtaactc acgatgagaa acccaacaat cccaccgggt
      181 gtgacggtgg agaacctgag cggcagtgac ctgcggcatg cccgcagggt gatgtaaccc
      241 gctgacaacg gggattgagg cgagatcact aagccgagat gatcctcaag gttaagtact
      301 gaaaggctga agaacatgaa cccgttaatc cgcctctgtg ggttgaaaac gtcaccacgg
      361 cctacgtgat ctgacaggcc gtgcaggagg aactggcagt gatacgtaag cactgccggt
      421 cgaaggtgtt ttgacatgta tgcgaaacac cggggcagca gcgtctatca cgcttgcgtt
      481 gctgacttct gccaacttgc ggcaagcaag gataaagagt gcgacgggca gcctcctcag
      541 tatgcctgag tccaggcagg taaaccgggg aaggtcagcg acggatgtta agggggcatg
      601 gctccgatga cgcgctgact ggcggagctt ccgtagtagt ccgcgatggg gaaagcccat
      661 tacatggcga agggaagcag tttgaatgtg tttgcgacgt gaattaactg gcctaacgag
      721 gtgaagacct ttgataatca gcgaaatgca acgcaagctt gccacatggg cagccaccga
      781 tccgtcccta cggattcaac ggctgctgcg tctgataaca caaccagaat ggctggctga
      841 agcggcgcgg atcacgcttt catcaaaggg ggcgcatacc cccggcgttg atggcgtgaa
      901 caaaacaatg ctacaggcca gactggctgt tgagctgcaa atcctcaggg atgaattact
      961 ctcaggccac taccagccct tgcccgccag acgggtttac atccctaaaa gcaacggcaa
     1021 actgcgacca ctgggtatcc ccgcgttgcg ggatcgtatt gttcagcggg ccatgctgat
     1081 ggcgatggag ccgatatggg agagtgattt tcatacgctc tcatatggct tccggcctga
     1141 gcgcagtgtc caccacgcga tccgcacggt gaaattacag ctcacagact gcggtgaaac
     1201 ccggggacgc tgggtgattg aaggcgacct gtccagttac ttcgacaccg tacatcatcg
     1261 actgctgatg aaggctgtac gccgcaggat cagtgacgca cgtttcatga ctctgctgtg
     1321 gaaaaccatc aaggcgggac atatcgatgt cggtctcttt cgggcggcca gtgaaggtgt
     1381 gccacagggc ggtgttatat cgccgctatt atcgaacatc atgctgaatg agttcgatca
     1441 atacctgcat gagcgctacc tgagcgggaa agccagaaaa gatcggtggt actggaataa
     1501 cagtatccaa cggggccgaa gtacggcggt cagagaaaac tggcagtgga aacccgcggt
     1561 ggcgtactgc cgctatgccg atgattttgt cctcatcgtc aaaggcacca aagcacaggc
     1621 ggaagccatc agggaggagt gtcggggtgt gctcgaaggc agtctgaaac tcaggctgaa
     1681 catggataag actaaaatca cccatgttaa tgacggcttt atctttctgg ggcacaggat
     1741 cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc tcaacgatcc cgcaggagaa
     1801 agccagaaac ttcgccgcat cgctgacagc actgttatca ggcaactaca gtgaaagcaa
     1861 agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc tgggccatgt tctataagtt
     1921 cgttgatttt aaggccaaag tcttcagtta tatcgaccgt gtcgtgttct ggaagctggc
     1981 tcactggctg gcccgcaaat accgtacagg tatcgcttcc ctgatgaggt ggtggtgtaa
     2041 atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt ggtaaaacca atcacggcaa
     2101 gctcagcggc gaaatactgt accggttggt ggggcaaggc aagaagctgt tccgctggcg
     2161 gctacccgaa ggtaatccct atctgaggac ggagaccaga aacacgtata catcgcgctt
     2221 tacagaagtg gcaatggcgt tcgccagcat ttaaatggag agccggatgc gctgaaaggt
     2281 gcacgtccgg ttcggggagg agaggcaggg aaatagtccg actacgccct gcctcttact
     2341 ctactcagca catgg
//
LOCUS       DADWTH010000244         2340 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.250, whole genome shotgun sequence.
ACCESSION   DADWTH010000244 DADWTH010000000
VERSION     DADWTH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.250"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..121
                     /locus_tag="JNY02_004223"
     CDS             <1..121
                     /locus_tag="JNY02_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435487.1"
                     /translation="QPKPPAPPAWAMMPPSNSLQLLDETFSVSGTESSATRQH"
     gene            complement(254..766)
                     /locus_tag="JNY02_004224"
     CDS             complement(254..766)
                     /locus_tag="JNY02_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBB9435488.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            961..1725
                     /locus_tag="JNY02_004225"
     CDS             961..1725
                     /locus_tag="JNY02_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435489.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQTLKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIGGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGECDLNDFYSDIQTDAESGFT"
     gene            1676..>2340
                     /locus_tag="JNY02_004226"
     CDS             1676..>2340
                     /locus_tag="JNY02_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBB9435490.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          663 a          521 c          592 g          564 t
ORIGIN      
        1 tcagccgaaa cctccagcgc ctccggcatg ggcaatgatg ccaccgtcga actctctcca
       61 gttgctggac gaaacgttct cggtatccgg gacggaatca tcagcgacca gacagcattg
      121 agaatgcttc aggagtacat caggatccaa tgccttgggg ggtagcggta attttactca
      181 ttatccttca aatcagattc tgttgtcaga ggaatggggg aagctggatt cgcaagagct
      241 ttttatgggc agtttagcta aaaaaaggca acacgaagct aaaactacca acataaagtg
      301 ctgaccagct agcagctgaa atcagactca ctgtatacac tttcactgga tgaagttgca
      361 acattcctgc caccaaagga gcaatgtaac gcagcacggc gataaagcgc gaagtgaaaa
      421 gtattgacac agagttattt tgcaactgaa ggcgaacccg ttctattgta ttggaacggc
      481 tcgatattat acatgcaagt tgagggatat gcccaattat tataccaaga tggtaattaa
      541 cgatagtacc gcaccatgcg ccactcatga ccgtgattcc agcttcccat ggtgaaagag
      601 tcgtcttgct gactgtaatt acagtggcca tcataattga tgctggtggc aatatcgccg
      661 aaatgagtaa agtggatttt gtgaacgcta ttataaaaag aagcccccat aggcgatatg
      721 gatgaagtac aaaatagttc atcaaagcat ttatccactc catcaatgtc tttccttctg
      781 tgaatttttc tttgctattt aaccgtgata cgacttaatt taggcttaaa caccttacat
      841 ttacaaggta ctcctgtggg gggccctgcc acggagcgtc gcaggagtga gtgatgagga
      901 agaattaaag caacctattg tgacaggcaa cgataacagt aaatcacagg aagcataatc
      961 atggcaaaac cggactggga ggccatcgaa tcggcatacc gggccggagt ccttagcctc
     1021 cgtgatatag gcgagaaata cggcgttaca gaaggggcta tcaggaagag ggccaaaaag
     1081 cttggttggg cacgcagtgg cggtacgcag gtttgcaaaa atggtacgca aaaaaggaaa
     1141 gtgcgtacca gcagaaagcc tgccattact ggccttacac aaaaaagtac gcaactaaaa
     1201 acagaatcta caccggatac gaaaccgata cgcggaatgc gtaccgatcc cccgactaac
     1261 ccattccaac ccggtaacca gcagacatta aaacacggtg gttatgcccg tcgccttctg
     1321 ctcaaagatg aggtgataga ggacgctaaa gcgttgacgc tcgaggacga attatttcgc
     1381 cttcgtgcta acaaccttgt cgctgcagag aatattggtc ggtggttggt gtcgctggaa
     1441 gatgctaatg gggaccagga aaggaagatg ctgattgaaa atatcagcgc cgccgagaaa
     1501 gcaatgatgc gcaatacagt tcgtattgag tccatcggtg gcacgcttgc gacggtagga
     1561 aaaatatttg ctgatacagc ctaccgcaag gccgccactg ataaggtgtc tctggaggct
     1621 gatcgtcttc gccgtgatgc aggtattgat gatggcaatg gagagtgtga cctcaatgac
     1681 ttctactctg acatccaaac cgacgctgaa tccggcttta cgtagtttct ggactatgcg
     1741 ggcacgtaac aaagtgcttt atggtggtcg gtcatcgtca aaatcatggg atgccgctgg
     1801 cattgccata tttctgtcga ataaatacac cctgcgtttt tgttgtgccc gtcagatcca
     1861 gaataaaatc gaagagtcgg tgtataccct gctcaaaatt cagatagaca ggtttggtct
     1921 gcggcaccgt ttccgtattc tgaacaacaa aatcattaac cgggttactg gctcggaatt
     1981 tgttttttat ggattatggc gcaacatcga agaaattaag tcactggagg ggatcgatgt
     2041 gttgtggctg gaagaagccc acgcactgac ggaataccag tggaaaattc tggagccaac
     2101 gatccgtaaa gagggttcgg aatgctggtt catattcaac cccggacttg ttactgattt
     2161 cgtctggcgc aacttcgttg ttgatccgcc cgaaggcact ctcatccgca aaattaacta
     2221 tgacgaaaat ccgtttctgt ctgacaccat gcttaaggtt atcgacgcgg cgcgacgccg
     2281 tgatccggat ggttttaaac atgtgtatga gggcgttccg gagtctgatg atgatgcggc
//
LOCUS       DADWTH010000245         2332 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.251, whole genome shotgun sequence.
ACCESSION   DADWTH010000245 DADWTH010000000
VERSION     DADWTH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.251"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..684)
                     /locus_tag="JNY02_004227"
                     /pseudo
     CDS             complement(1..684)
                     /locus_tag="JNY02_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            870..1712
                     /locus_tag="JNY02_004228"
     CDS             870..1712
                     /locus_tag="JNY02_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBB9435491.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1715..2320
                     /gene="capU"
                     /locus_tag="JNY02_004229"
                     /pseudo
     CDS             1715..2320
                     /gene="capU"
                     /locus_tag="JNY02_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
BASE COUNT          625 a          469 c          535 g          703 t
ORIGIN      
        1 tccgttacgg cgaactgtcg ctcaagatga ttcgggatag caacgtgctc atgaccgcca
       61 cgcttatacc ggtgagtcgg ctgctggcaa ctgaccagcc ccagctcttt catgagtctg
      121 ccagcaagcc agcgccccat ctggtagcct ctctgggttg ccattgtggc gatgcttctt
      181 gctccggcag agccgtggct gatgccatgc agttcaagta cctggctgcg taatacagcc
      241 cgtctgccgt ctggtttttc aggacggttt ttccagtatt tgtagctgct gcgatggacc
      301 ccgaacacat ggcagagagt ggccacagga taacgcgccc tgagtttccc gattatcgaa
      361 aactgttcag ggagtctgac atcaagagcg cggtagcctt tttaatattt cattttccat
      421 ttcaatacgt tgtagctttt tcctgagctc acggatttca atttgttccg gggtaatggg
      481 ggaggctttt ggtgtttttc cctgccgctc atcacgtaat tgtttcaccc atcgcgtcat
      541 tgtggaaagg ccgacatcca tagcgctggc tgcatctgcc acggtgtatt tctggtcaac
      601 gaccagttga gcggattcgc gtttaaactc tgcgctgaaa tttctttttt cattatggca
      661 cctgtgttgt tctgaggtga gcatatcacc tctgttcagg tagctaaatt tagtgtgcca
      721 cctcaaggtt cttagtgagc cgtttttaaa agatatgata atatattatt gtttataaat
      781 tgttgatgtt tgatttagta gtatataaag ttcaccacct ttattaggct ttccttttat
      841 ctctacttac tgggtgccag aaactttaaa ttttaaacgg agggggcatt ttgtttaaag
      901 caaatcatgt tccggtatta atgtatcatc atgtcagcca ttgcccagga ctggttacac
      961 tttctcccga gacattccgg aaacaaatga aatggttggc tgaaaacaac tggaaaacac
     1021 tgtcatcaga tgaactggag tttttttatc ggggcggaaa acttccccgt aaaagcgtta
     1081 tgttgacatt tgatgatgga taccttgaca actggtttca ggtatatcct ttattaaacg
     1141 agtttaatct caaggcacat atttttttaa ttaccagttt tattggaaat ggtccggttc
     1201 gtcactctcc tggaaaggaa tattctcatc gcgattgcga acatcaaata gcaaccggga
     1261 atgctgataa tgttatgctt cgctggtctg aagtgaacga aatgttgcaa tctggtctgg
     1321 tcgaatttca tgttcataca catactcata cacgttggga taaaaagttc acttcacgag
     1381 aagaacagtg taaacatctt cggcaggact tattgagcgg gagagaatat ttaaaaaaaa
     1441 tgacaggaaa atgcagtaaa catttgtgtt ggccggaagg ttattataac aaggattata
     1501 ttcagatcgc tgaagaactt ggattccatt atttatatac aacagaaaga agaatgaatg
     1561 ctcctgctaa aggaactaca cggataggaa gaataagcac caaggaacgg gaaaattgcg
     1621 tatggctgaa aagacgactt ttttattata caacaccgtt tttttcatcg ttgctggccc
     1681 ttcataaggg gccaagatta ccggatgact aataatgaat atactattta cggaatcatc
     1741 accgaatata ggtggtcagg aattacaggc tgttgctcaa atgaaggccc tgaagaaaat
     1801 ggggcattca gttctgcttg tctgcaggga gaacagcaaa attgcttttg aagccagtaa
     1861 gtttggaatt gatatcacat tcgcgttatt tcgaaacagt cttcacattc ctactgtatg
     1921 gagattactc ggaatagttc atagttttca gcccgatgca atcgtttgtc acagtggaca
     1981 tgatagtaat attgttggtt tggtacggtt ttttacctgg aaacatccat tcagaattat
     2041 caggcaaaag acatatttga cacgaaaaac aaaatttttt tcaataaatc atttttgcga
     2101 tgaggtgatt gttcccggaa caaatatgaa gacacatctg gagcaggaag gatgtcgaac
     2161 tcgggttact gttgtgcctc ctggctttga tttccaggaa ttatacgttg attcccgaaa
     2221 cagtttgcct ccgagtgttc tttcctggct ggcgtcccga agaggatgcc ccgtaattgc
     2281 tcaggtagga atgttgcgcc cggaaaaagg gcacgaattt aggtgatgct gc
//
LOCUS       DADWTH010000246         2302 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.252, whole genome shotgun sequence.
ACCESSION   DADWTH010000246 DADWTH010000000
VERSION     DADWTH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.252"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..784)
                     /locus_tag="JNY02_004230"
     CDS             complement(<1..784)
                     /locus_tag="JNY02_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBB9435492.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1132..2004
                     /gene="rluF"
                     /locus_tag="JNY02_004231"
     CDS             1132..2004
                     /gene="rluF"
                     /locus_tag="JNY02_004231"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBB9435493.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2137..2302)
                     /gene="yjbD"
                     /locus_tag="JNY02_004232"
                     /pseudo
     CDS             complement(2137..2302)
                     /gene="yjbD"
                     /locus_tag="JNY02_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
BASE COUNT          562 a          589 c          577 g          574 t
ORIGIN      
        1 cgctcatatt cgtttcaacc attgcgccgt gaatatcaaa aaagcgcgag caaatatcgc
       61 cagcaacctg gcgggcattc aggtcgtcac tctcttcacc accataaaac gcatgccagt
      121 tagcgccgtc gcgaatggcc ggtgagccaa ttcccaccag ggcgacatcc agattatccc
      181 agtaggcaga gatggtttta aagtgctgag actgcatgat cccattacga attaatgggt
      241 tatccagcag agccggaaaa tccgcgagat gcgattcccc tttcagcttc gctgccgcgc
      301 tgtaggttaa tgtgtttaca tgatagcggc tttcgagttt accggacggt ccgccaataa
      361 ttggtacgca gattaactgc cgcgattgcc ccgcctgcgg caagttttca accagtgcgc
      421 tcaccgcgcg accccaggaa aaaccgacaa tatcgccagg ctccagcaag cgatccagca
      481 gttgcgcgcc gtgtaacccc atcatcgcca gttgagtctc ttcatcctca tcatttcccg
      541 acaccaccac aacgtctttc aggccaaact tttgcttcag ttgctgctcc agccagagat
      601 tttcgttgta gtcatagttg atggcgatgg tgaccattcc ctgatcgcgg cctcgtttaa
      661 gcaagcggct gatggtggtg cggtaaatac ccagttcgcg cgcgatttgc gcctgcgtca
      721 tatcctgttc gtaatagagt tgggcaatct tcacaatcaa acggatatcg tcactgtttt
      781 ccattgccgc ctcctggttc tgcacatttg tgcaaatacg ctttctcacc gcttgatctg
      841 atataacccc atcatgaggg cctttcaaag cgatttttca ctctttgcat ggggagaaat
      901 agcgctttct cttgcacatt tgtttctctg cgtagtagtc atcagcacag gtgtgcagcg
      961 attcgtaata tgttgattat ccttattttg ccaggcaaat ttgcatcgta cttccggaga
     1021 cacttttgtg ataaaaggcg tggcagatca catttaattt cgacgttgcc gcataaccct
     1081 gtttttcccc gctaagaggt acggtatatt ccgcgtttta caggagaatt tatgctgccc
     1141 gactcatcag tccgtttaaa taaatacatc agcgaaagcg gaatttgctc acgccgcgaa
     1201 gcggatcgct atatcgagca aggcaatgtg ttccttaatg gcaagcgagc caccattggc
     1261 gatcaggtga aacccggcga cattgtgaaa gtaaacggtc agttgattga gcctcgggaa
     1321 gccgaagatt tggtacttat cgccctgaac aagcccgtcg gtattgtcag caccaccgaa
     1381 gatggcgagc gcgataacat tgtcgatttc gttaaccaca gcaaacgcgt gttcccgatt
     1441 ggccgcctgg ataaagactc ccaggggctg attttcctca ccaatcacgg cgatctggtg
     1501 aataagatcc tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa
     1561 ccgattaccg atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg
     1621 acgaaaaagt gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag
     1681 gggctgaacc gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg
     1741 gaacgtacgc gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat
     1801 ttaaccgacg atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca
     1861 aaacctaaag cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg
     1921 gaaaaaacgg cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg
     1981 gggcgtaaaa agaagggacg ctgattaacg cctcagactg atgaaaacgt ccaaaaagag
     2041 ctcggacagt cccctcgccc cttcggggag agggttaggg tgaggggaag attgtgtcca
     2101 cacagacgag tgaaaatctc aggcgttgat tgttcattag cgcctgccgc gcgtcgacgt
     2161 atttgctgac cagctaaatg acgcttcagg atccggttta taagcctgct ttttcttcaa
     2221 ttggcgggct tttttcgctt cagcctcacg ctcgaccatt aatttatcga tgtactctct
     2281 ttcttaatgc tgttggtttc gg
//
LOCUS       DADWTH010000247         2237 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.253, whole genome shotgun sequence.
ACCESSION   DADWTH010000247 DADWTH010000000
VERSION     DADWTH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.253"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..171)
                     /locus_tag="JNY02_004233"
     CDS             complement(<1..171)
                     /locus_tag="JNY02_004233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435494.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..1735
                     /locus_tag="JNY02_004234"
     CDS             308..1735
                     /locus_tag="JNY02_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBB9435495.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNSTKNAAFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLN
                     YGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSA
                     DNVDGVYYRPLQKLINGTWYNVASI"
     gene            1738..>2237
                     /locus_tag="JNY02_004235"
     CDS             1738..>2237
                     /locus_tag="JNY02_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBB9435496.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSS"
BASE COUNT          612 a          523 c          620 g          482 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag
     1321 cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg ggtaatccat ccaggcctgt
     1381 tgttgcagag cttggcgatg attcaggctg gcattttttc agccagagaa acccggataa
     1441 cagcatagtg ttcactgtta acggacaggt aattccgtta aattacggaa acttcgatgc
     1501 ccgctataaa tatcgaacag agggggtaca ggatgtacgg tatggccatg aaatgtatta
     1561 cagccccggc agtaacaccg tttcgtggag attttgcgca ccttcgggac acgggctgtc
     1621 agggatggcg atatcggata ccggccgtaa ctcagcggat aacgttgacg gtgtgtatta
     1681 ccgaccactg caaaaactga ttaatggcac ctggtataac gtagcgagta tttaacaatg
     1741 ttgcatttaa aaaatattac tgcgggtaat ccgaaaacag cagaacaata tcagatgaca
     1801 aaacgatatt cggtcacctg gcttttttca gaagacggaa aaaactggta tgaagagctg
     1861 aagaatttcg ccagcgacac aataaaaata gcttacaccg gagatggtcg cgtggtgtgg
     1921 gtcggtaagg atgtgacagg catcgagcca cgcaatgcca gcgttatcga agttccggat
     1981 attaccgcta accgacggat taccgcgccg ggttactggt tttaccgcaa tgatgaattt
     2041 gtctttgact acagactcaa agcggaagat gagcgtgatg cccttctggc tcaggtcagt
     2101 gcccggacag gggaatggga agaagacctg ctgctggggt taatcagcga cgaagataaa
     2161 gaaaagctga aagcctgtcg tatttacgcg aaatcgctgc aggcgatgga tttcagcacc
     2221 atcactgata aatcctc
//
LOCUS       DADWTH010000248         2186 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.254, whole genome shotgun sequence.
ACCESSION   DADWTH010000248 DADWTH010000000
VERSION     DADWTH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.254"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(220..384)
                     /locus_tag="JNY02_004236"
     CDS             complement(220..384)
                     /locus_tag="JNY02_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435497.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1285..1569
                     /locus_tag="JNY02_004237"
     CDS             1285..1569
                     /locus_tag="JNY02_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBB9435498.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1557..2042
                     /locus_tag="JNY02_004238"
     CDS             1557..2042
                     /locus_tag="JNY02_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB9435499.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          461 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaaggaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg
      481 aaaatgtgct atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg
      541 gcatccggca tataggcagc atgaacgcaa cacaagctct cgtacggaac gtgggaacct
      601 gtcgtctcga tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga
      661 tgcgagacac aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga
      721 gcgaaggggg tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc
      781 atgagtcagc caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg
      841 gtcaaagcta atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt
      901 accaatcgaa gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg
      961 cccgcaaaca caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa
     1021 ttcgtcacga ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg
     1081 ttcaacgaca agagccgtat gacgcgagag tgtcacgtac ggttccgtgg gagcgtaggg
     1141 gggcggtgcc cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa
     1201 atgccgctga cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt
     1261 caatatgcta ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag
     1321 ctcaatctca gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt
     1381 cataagtcgc gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt
     1441 ctcgaccggc gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt
     1501 gatgctccgg ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg
     1561 gatgtgtaac tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagcg
     1621 gtgaagctgt acttgatgac tggttaaagc agaaggggct aaaaaatcag gcaatcggag
     1681 ccacccggac ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac
     1741 tggccactgg tagtatcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag
     1801 accccattcc tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag
     1861 ggcttggcgc ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata
     1921 tcggtgtgcg tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc
     1981 atcatggttt taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat
     2041 agggagtaat gccgatcagt taaggatcag ttgaccgatc cagtggctgt gtaagaatcc
     2101 ggaaacgctc actcgtttcc ggatttttct gtgcacagta aaagaccacc tcctgaggag
     2161 gtggcttttc agaatgcccc ctataa
//
LOCUS       DADWTH010000249         2151 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.255, whole genome shotgun sequence.
ACCESSION   DADWTH010000249 DADWTH010000000
VERSION     DADWTH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.255"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNY02_004239"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNY02_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HBB9435500.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JNY02_004240"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JNY02_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JNY02_004241"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JNY02_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..>2151
                     /locus_tag="JNY02_004242"
     CDS             1905..>2151
                     /locus_tag="JNY02_004242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435501.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          633 a          394 c          491 g          633 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DADWTH010000250         2143 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.256, whole genome shotgun sequence.
ACCESSION   DADWTH010000250 DADWTH010000000
VERSION     DADWTH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.256"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..213)
                     /locus_tag="JNY02_004243"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="JNY02_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            488..792
                     /locus_tag="JNY02_004244"
                     /pseudo
     CDS             488..792
                     /locus_tag="JNY02_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            961..>2143
                     /locus_tag="JNY02_004245"
     CDS             961..>2143
                     /locus_tag="JNY02_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9435502.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          555 a          442 c          492 g          654 t
ORIGIN      
        1 tgaacctcga tcggtgtggt ggattagccc ggcaggtggg cgctggctcc tgagcgccat
       61 aaacagggct ttacccgtca actctttcgt catgcgctct cccatggcgt aaccgacaat
      121 ttcgcacgta taaacatctt tgacgccagc gagatacagc catccttccc gggtggcaac
      181 atacgttata tctgcgaccc agacctgatt aggggggttg gggagcaatg gaacagtaaa
      241 cgccgttaag aagtcaattt aaatagccaa aaattaacta gccaatttac attgcaggtt
      301 gtaattttta accacctttt ttacgatagc caaattacct atacccatcc cggccctgcc
      361 aagctatacc cgctatcggc gatgaaaatg cttttgtgcg cggtcggagt ggtcccgacg
      421 agggttttac ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg
      481 agcgtgtatg agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga
      541 aataaaacca gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact
      601 acaagatttt atttactggc gtcctgatgc ggacaggact ggtatggaac ctgtttatgt
      661 gatacttaat gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat
      721 cttaaacatg caagtaattt tggtattctc gatacaagaa taaatagtag taactactat
      781 agttgcaaat aatgttaaaa acatctctat gatcatcttt acatttttca tgattgacaa
      841 ttgtttttgg ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta
      901 tataatgata agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat
      961 atgtcattat tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga
     1021 ttatatcccg tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat
     1081 tattttgtcc gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag
     1141 atcggcatta tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga
     1201 cttatttctg acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga
     1261 ctggtaaatt ttctgatagg atatattccc gggatcatca catccgttac gttattttca
     1321 acaatgtatt tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca
     1381 ctggtgttct ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg
     1441 cacaatgtcg ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc
     1501 gacagtgctc tggagcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca
     1561 atgagtgtgc tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc
     1621 attgaagaat ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg
     1681 agactctcca ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac
     1741 tgctgtatct atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct
     1801 aagtttttaa gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg
     1861 ggggggtttt ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg
     1921 tctgtcagac tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc
     1981 attattttat taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac
     2041 tatacaatcc ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt
     2101 tattcaatgg agcttgttcc taaagaagct gccggagccg cat
//
LOCUS       DADWTH010000251         2136 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.257, whole genome shotgun sequence.
ACCESSION   DADWTH010000251 DADWTH010000000
VERSION     DADWTH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.257"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            14..487
                     /locus_tag="JNY02_004246"
     CDS             14..487
                     /locus_tag="JNY02_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB9435503.1"
                     /translation="MRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDELYQV
                     YSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKLALMA
                     MEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNY02_004247"
     CDS             complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNY02_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBB9435504.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1111..2124
                     /gene="argF"
                     /locus_tag="JNY02_004248"
     CDS             1111..2124
                     /gene="argF"
                     /locus_tag="JNY02_004248"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBB9435505.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          544 a          520 c          532 g          540 t
ORIGIN      
        1 gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat
       61 ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc
      121 gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga
      181 atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga
      241 catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa
      301 aaaactggcc ttaatggcga tggagcaggc tcgagagatg ggcttcaaac gctgctatct
      361 ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca
      421 tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga
      481 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
      541 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      601 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      661 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      721 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      781 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      841 ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      901 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      961 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
     1021 acaaagattt atgctttaga cttgcaaatg aataatcata cacataaatt taattttaat
     1081 tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg
     1141 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
     1201 aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
     1261 atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag
     1321 ggtgctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt
     1381 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
     1441 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1501 aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc
     1561 ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc
     1621 aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1681 gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1741 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1801 accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta
     1861 ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1921 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1981 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga gtctgccgcc
     2041 agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg
     2101 acgctcagta aattaaataa ttaatatctc tataaa
//
LOCUS       DADWTH010000252         2128 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.258, whole genome shotgun sequence.
ACCESSION   DADWTH010000252 DADWTH010000000
VERSION     DADWTH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.258"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(246..443)
                     /locus_tag="JNY02_004249"
                     /pseudo
     CDS             complement(246..443)
                     /locus_tag="JNY02_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(575..2098)
                     /locus_tag="JNY02_004250"
     CDS             complement(575..2098)
                     /locus_tag="JNY02_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBB9435506.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          636 a          467 c          451 g          574 t
ORIGIN      
        1 atgacattga tcactttggg gtgactattt tgtttaacaa ataactcttc gacatcaaaa
       61 ttattaatag ttgccatggt aattatttcg gtgatatata aaaacaactg tgataacacc
      121 aaggttgaca actatttgcg caataattcc tttccttcaa tgatccaggc gttcactgcc
      181 agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc tgatccctgt
      241 ggctgacgag tctttctctt aaatcgatta accagattga tcgccagcca aacaagggca
      301 ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt tgcaataagg
      361 atgaccagga gggtgatcag cataaatgca atggaaaaga tgatatagag agtgaaataa
      421 tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact ttcaaacaat
      481 tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt agctgtctat
      541 aattacaccg cactatggcc ttactgatca attttcactc aaccacaacc ttcaccttcg
      601 gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag aaataataac
      661 cctgctgaaa tgtgatatta ttttggttca gatagccaag ttgttctttc gtttcgacac
      721 cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa tccagaattc
      781 gggttgattc tgggtctgcg tttacgcggc caacgaaact gtgatcaatt ttgatataat
      841 caatatgcag gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa tcatccagcg
      901 cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt tcatcaacat
      961 tcaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg gtaaaactgt
     1021 cacggaaatt taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa gcgctaaaat
     1081 taattccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc tgtctggcaa
     1141 cctgattcat caggctttgt gtaagcggaa cgattaatcc ggatttttca gcaagtggaa
     1201 taaatgccgc gggtgaaata tatccaccgt gaggttgttt ccagcgggct aacacctcaa
     1261 ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg acgatctccc
     1321 cattatttat tgcccgtcgc aggatctctt caggcgttgc gcttttattg aaatatctat
     1381 caagcaggaa ggcagcggcg caggcgatta acagaataaa tattaaaatc ccaaagcctt
     1441 tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata gtgaaatgat
     1501 attttgtgga gtgaaccttc tcggtatagg gataatgtcc attaagcgtc atcacatcac
     1561 ccgttggtcc aataatgtca tccgccacgc gtaatacata ccttacccct ttcaagggta
     1621 cattaagcgc cccgcgaata tgttgatcac ttatcgtaac caaaatgcgc gtatctgcaa
     1681 attggctctg atagagcagg ataggtaatc tattaacggt gtcgattgcc ggagccaaca
     1741 gtaaattact gtccgggaca acaggaatac gagacagcag gacccgattc ccaggcaggg
     1801 atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag
     1861 ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg gcaatgttaa
     1921 ccgctgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg ttggcggcat
     1981 atcttgctcc cgccaggtac tctgcttttg cggaatacca gagctggatg ttcaaaatag
     2041 cgacaccggt aataaacaag catatcgcaa cgaggatggg tatgagtgta ttgcgcattt
     2101 gcttgtcccg gccaaagggt ttaataca
//
LOCUS       DADWTH010000253         2118 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.259, whole genome shotgun sequence.
ACCESSION   DADWTH010000253 DADWTH010000000
VERSION     DADWTH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.259"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            129..1166
                     /gene="curA"
                     /locus_tag="JNY02_004251"
     CDS             129..1166
                     /gene="curA"
                     /locus_tag="JNY02_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBB9435507.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1364..2029
                     /gene="mcbR"
                     /locus_tag="JNY02_004252"
     CDS             1364..2029
                     /gene="mcbR"
                     /locus_tag="JNY02_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBB9435508.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          583 a          454 c          567 g          514 t
ORIGIN      
        1 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
       61 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      121 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      181 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      241 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      301 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      361 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      421 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      481 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      541 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      601 cgacaggacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtga
      661 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      721 tttgccttga ttaccacgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      781 gtattgatat ctattatgaa aacgtgggcg gcaaggtatt tgatgcagtg ctaccattac
      841 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
      901 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
      961 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1021 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1081 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1141 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1201 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1261 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1321 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1381 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1441 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1501 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1561 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1621 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1681 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcaggcgc aagaaaaggg
     1741 cgacatggaa caaatcatta atgcgaacag gctatttcgc ttagcgattt atcatcgctc
     1801 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1861 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     1921 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     1981 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2041 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2101 taatttcgtc cgggcgca
//
LOCUS       DADWTH010000254         2088 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.260, whole genome shotgun sequence.
ACCESSION   DADWTH010000254 DADWTH010000000
VERSION     DADWTH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.260"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(60..1025)
                     /locus_tag="JNY02_004253"
     CDS             complement(60..1025)
                     /locus_tag="JNY02_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBB9435509.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1878..>2088)
                     /locus_tag="JNY02_004254"
     CDS             complement(1878..>2088)
                     /locus_tag="JNY02_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435510.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          575 a          548 c          437 g          528 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gtgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac ccccaaaaca agcacccccg aacggcttac cccaaccttt
     1381 tccacgcacc cgatcaccaa aacagggaat aaaaacaaaa ccctgtctta caggtggatg
     1441 gcctcacttc gtgaaaccat ccaccccgca taagtttttt catcggtggt gtcgtcaatc
     1501 actaaatcgg aaccccaaag gggaccccca cgatttagca tttgacgaga ttaactctcg
     1561 gagatggcat catgctttta acggacttta aaaaacggtg aacggatgcg gattttaatc
     1621 cgtgtctgtg aggcgttgag caacgagcaa agcgaggcgg tagaaagaga gccagcggca
     1681 ggctcaggac gataatccgg ggaaaatgta ccactcgcag agtgttaaca tttgacatcg
     1741 gattgtctat cctgcattgg tgtcgagttg gcaaggctga ggataatgct gaggataatg
     1801 caggatagac agagcactag agattgcatg caatctcgta cctatcacct cagcctgccg
     1861 ctggctcttt tccagaacta agaatgcgtt cttaaaaatc caaacttgat accgtttaaa
     1921 tctattttca caaaaagagt ttttaccctc tcgtttgttt tctgaatata taaaattaat
     1981 tcatcctcaa aaccttcgtg cggatgaaaa ccaaatgtgc caaaacttaa tccatcaata
     2041 agcaaaataa ggtttttaaa cctgtcagga atcactgctt cacttctt
//
LOCUS       DADWTH010000255         2087 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.261, whole genome shotgun sequence.
ACCESSION   DADWTH010000255 DADWTH010000000
VERSION     DADWTH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.261"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..799
                     /locus_tag="JNY02_004255"
                     /pseudo
     CDS             1..799
                     /locus_tag="JNY02_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="JNY02_004256"
                     /pseudo
     CDS             914..1255
                     /locus_tag="JNY02_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="JNY02_004257"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="JNY02_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          505 c          631 g          436 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc ggtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggt cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acttggtgat gaagtctctt gatcaggagc tctgatcaag agacagc
//
LOCUS       DADWTH010000256         2078 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.262, whole genome shotgun sequence.
ACCESSION   DADWTH010000256 DADWTH010000000
VERSION     DADWTH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.262"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..258
                     /locus_tag="JNY02_004258"
                     /pseudo
     CDS             1..258
                     /locus_tag="JNY02_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            679..1257
                     /locus_tag="JNY02_004259"
     CDS             679..1257
                     /locus_tag="JNY02_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435511.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1366..1624)
                     /locus_tag="JNY02_004260"
                     /pseudo
     CDS             complement(1366..1624)
                     /locus_tag="JNY02_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1608..2078
                     /locus_tag="JNY02_004261"
                     /pseudo
     CDS             1608..2078
                     /locus_tag="JNY02_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          671 a          377 c          508 g          522 t
ORIGIN      
        1 ggggatttaa attcctatcg atgaactgtc gatatgaaaa agcgagcatc atactgacgt
       61 caaacaaagg ggtcgcagac tggggagaaa tgttcggaga tcacgtgctg gcaacagcga
      121 tcctagatcg gctgctacac gctgaatatc aaaggagaga gttaccggtt aaaagagaaa
      181 cgtaaagctg gagtgctgac caaaaacaca acgccaatca gtgatgatga aatggtggaa
      241 aacggacagc atcagtaacg aaagtatctt agcaggcatg aaaatcgtcc gcatccccta
      301 aacggtcaaa atgcttctgc cgtgaaggag ggagaatgcc atctcatcac ttatatctgg
      361 cgaatgaaac acagatggtc ggccttgccc ccaaacacac ggcggtgcaa aatggaatgg
      421 cagaaaactt cgtcaaaacg atgaaatatg attaaatcag tgttatgttc gaacctgacg
      481 tgttaacagc agtaaagtag tctcgcgaag gtgttcaaac attataatga atggcatcct
      541 catagtgcgc agggttatcg cttgctacgg aaatatctga agcgacgtac cagtaatggg
      601 gaaaatgata gctggtttct gaaaatatag aggaaaatgt gatatatata tcctgttaat
      661 atttaaggag aatataaaat gaaagtctcg tttaagtctt taggctatat ttttcatgat
      721 atatataata aaaaacacac tatcgatgag ttcaacgatg tggtaagaaa agcagtttta
      781 tcaggtaaaa ttaacgagct gaatgcatgt cataaagtgg caatttttct tgccgaaaaa
      841 gacaatgaga taacaaagaa agacaaagcc aagataatag atacattaac tgaaaactat
      901 tccattgaat ttcaacaatt aatgaatatt agtgagcgca cgttaaattc atctttatat
      961 attactcctg gtgaaagtgg ttttgtcagt tttgttaatc gtgaaggaaa aatatgtcat
     1021 actgcatatg taaaatcatc agataatagc atggcatatt accatgcaaa tggtagcagc
     1081 attgacaaat atattacgga tatgtgcggc cttatttgta tgaggcatat tgagtctact
     1141 ggcattattt tttatatgct tgatgaaaaa gttttatctg ccattgctga atttatgaat
     1201 gaaaaaggat ggcgggctgc gttttgttca gcaaagaact tatataaatg tgtttaattt
     1261 tagaaaaact atttggtaaa atccaacaag agtcgagagg ttcgcgttgc ttcagcaggg
     1321 cctcagcctg ttcaaccaga ctcagttgca ggtcgtgacg cagatttagt ggtggtggtt
     1381 gattgtcaga taccagccga tgatcttgta gtgcatatca tcagactttg agaagcatat
     1441 ggttttgcgg gtaagccgct taagatgtgt ccgcaggttc agattatgtc gctctatccg
     1501 ctgagtgcat ttcttgctta taacatgact ggttgaggac aataaagttc ggtataccgg
     1561 ctcaggcatc ggtcatgtag aaggcaatgc taaactggct cagcaaacta agagcagcgc
     1621 gcatccactt acccaatgcg gttgagaaaa taaccttcga tcgctttcat gtggcgaaac
     1681 aaccgggtga ggtggtggat aaaacccgcc agaatgaaca tccgcatctt cctgttgaaa
     1741 gtcgtcgtca ggcaaaggga actcgcttcc tgtggcagca cagcgataaa tggatgaccg
     1801 aatccaggca ggaaaagctg atatggctgc gtgcgcagat gaagctgacg agcctgtgct
     1861 gggcgctgaa agaactggca aaggatatct ggagcagacc atggagcgag gaaagacgca
     1921 acgactggca gagatggctg cggccaacag tgacgtcccc atgatgaaga atgtggctaa
     1981 aacgataggg aaaaggttgt acggtatcct gaacgccatg cgccatggcg tatcaaacgg
     2041 aaatgcggaa gcactgaaca gcaagatcag gctgctga
//
LOCUS       DADWTH010000257         2062 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.263, whole genome shotgun sequence.
ACCESSION   DADWTH010000257 DADWTH010000000
VERSION     DADWTH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.263"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..123
                     /locus_tag="JNY02_004262"
     CDS             <1..123
                     /locus_tag="JNY02_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435512.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(272..1726)
                     /gene="ospC2"
                     /locus_tag="JNY02_004263"
     CDS             complement(272..1726)
                     /gene="ospC2"
                     /locus_tag="JNY02_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBB9435513.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          646 a          367 c          337 g          712 t
ORIGIN      
        1 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
       61 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      121 taagggacac agcctagtct ccaggattcc cggggcgttc accacatgca atgtgttgtt
      181 gcaggggaac ggtctgcacc atcccctata tcgatcaata taacaaaaat gacaacgtca
      241 gattcagggc acaacctaaa tatattttag gtcacctccc aaatcgtttg cctgataccg
      301 ctccattttt taataaaaaa tcaattgttt ttgaatcttt gcttttcatt gcattgtcaa
      361 gcatagtgtc cccactattt gctttttgaa atcttttgtt tacatcaacc aatccattat
      421 taataaaata ttctagcact ttatagttgg cactatcttc actaagtaaa tactcaacat
      481 catacagctc acaatacata ttgttgttca ttttctccat ctctgcgaca tcttgttttg
      541 taaaattaaa tttagatagt aaataaagtg ccatatcaga ttttgcattg cctagagaat
      601 aagcaagggc ctgatgagcc atttttttat cagtcacctt attgttaatt tcttcataat
      661 tggatgcttt aatggcatcc tccaaagata tatctctgag tcttatcttt ttgaagttat
      721 ctgtgcttag cattcttggt atatgatact caagctgaaa cacaaagttt ataattctat
      781 ccagactaac aggatcaatg tttttattat aacaaaactc tcggaatctt tgatctttac
      841 tctttcttat aaaatcaata agatgcaacc caattccaag cctcatatct tttgtattaa
      901 atattggcac atcagttttt ccctgattac catgcacata cctgctaact tcatccacaa
      961 cctctttaaa gttatctaag aaaaatgatt ggtgctcatg gtaaaaaaca ggtggaatag
     1021 cattatcaaa gtggtcggtt aatgtcatat atccatatgg agagtcatga tcaatgatat
     1081 acgcattagc accaaaatcc acagtatgat gtgttttatt tagtgggagt gtttcttgat
     1141 gattggaaat ttcaacccca aaaaaaacaa aatcgtggtt agctatttct tcaatatcct
     1201 cagagaatgt gtttttatat gtttgtatat tctcagactt aattttatca aaggacttaa
     1261 tattaagagt attatttgac aggttagcgt tactttgatg tctgaagtta aaatcagaat
     1321 taataataat agagagcaag tgacgttcat ccgaagaaag aggatgagtt ttttgtatta
     1381 tttcagaaag atagtgtctg gacaccttat cgaggtgctc aagtgaccga aatgatgacc
     1441 gtgctccaga tggcgcttca atagaaagat ttaatgattt gaaactggtc ccttgagaga
     1501 acattttact ataactttgc gctgctatag acttccttaa aaagcttttc acagtatcag
     1561 cacagtgatc taaatgtttt tcacttatgc cagaagagga tgagtttgtt acgcatgata
     1621 tgtttttttg taatgctttt gtatctttgt tcggatttat ttttccatca acaagatcaa
     1681 tattgttctg aacattaata tgatttactg cttcaggtat tttcataaaa catcccccat
     1741 tatgtgcgtt tttatctgca gttatttaga ataatattat ctaacttaat attttcttgt
     1801 atgtattata acgaacatat tttaatatgt tgtacgattt tcactctgaa atcccctgag
     1861 tttaatcaca cttcctttac tgaaaggaat tattgcaaca gtattatttg tttttggata
     1921 tatatcttgc gccttgttat gtagttaatg ctcagaatat gatagataaa atagagacgc
     1981 aaaacagggc atgctcataa catgatgata acataaaaaa ttagtaacaa taaatgaaag
     2041 aatcatgggc gctcaacaaa aa
//
LOCUS       DADWTH010000258         2059 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.264, whole genome shotgun sequence.
ACCESSION   DADWTH010000258 DADWTH010000000
VERSION     DADWTH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.264"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..1501)
                     /gene="pykF"
                     /locus_tag="JNY02_004264"
     CDS             complement(89..1501)
                     /gene="pykF"
                     /locus_tag="JNY02_004264"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBB9435514.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          523 a          457 c          497 g          582 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac
      361 cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac
      421 cacggcatac cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc
      481 tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg
      541 ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg
      601 cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca
      661 gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct
      721 tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca
      781 tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga
      841 ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga
      901 taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac
      961 caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag
     1021 gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt
     1081 tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt
     1141 tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc
     1201 cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt
     1261 taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg
     1321 cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg
     1381 catagtcacc atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag
     1441 ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca
     1501 tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc
     1561 cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc
     1621 gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg
     1681 agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt
     1741 atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg
     1801 ttagacaata aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt
     1861 cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc
     1921 cggtgtataa atatatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg
     1981 tctgattttg tttgattttt gtttaattgg ttagattgat atatgctgat agttggcatg
     2041 ataaataggt ataaagaat
//
LOCUS       DADWTH010000259         2044 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.265, whole genome shotgun sequence.
ACCESSION   DADWTH010000259 DADWTH010000000
VERSION     DADWTH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.265"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(55..324)
                     /locus_tag="JNY02_004265"
     CDS             complement(55..324)
                     /locus_tag="JNY02_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBB9435515.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            381..1049
                     /locus_tag="JNY02_004266"
     CDS             381..1049
                     /locus_tag="JNY02_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB9435516.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1365..>2044
                     /locus_tag="JNY02_004267"
     CDS             1365..>2044
                     /locus_tag="JNY02_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBB9435517.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          614 a          447 c          382 g          601 t
ORIGIN      
        1 cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat
       61 tgtgtctggt tcttccggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag
      121 caacacccga tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc
      181 cagatctaca gcatcctgaa gtggcgcaat atgctcactg aatccctgga tgtagaactg
      241 tgtggtgacg gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc
      301 aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa
      361 ttcacaaata aaactataat atggtgaaat taatgaaaaa aaacacagat gatggggcta
      421 aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc
      481 ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag
      541 gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga
      601 gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg
      661 ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcctgcgg
      721 cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa
      781 atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact gatgatggaa
      841 ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac
      901 tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct
      961 taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag
     1021 taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac
     1081 gtgctacggt ttcctttacg atttggggtt gcgactttac ctctattgat aatgcattcc
     1141 ggcagaacgt tcaaatatga acgtacgata tttaactaac cgaaaaacaa aatatagcac
     1201 aggcgagaca tttaccatct aagaaaatag tatcgttttt ttacagttaa atcaacatca
     1261 cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca ttttatttca gcttattatt
     1321 ccccagtatc acattttatc aggatatcct tctgcgaggt tattatgctt cctgtaaata
     1381 atccccccct atccactgga aacgtctctt tttacagaac tacatcaatc gacaatgttc
     1441 acaataatta tctctccgaa tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca
     1501 gggaaactgc ttttacccgg ctccaattat gtctggagaa cagtgaaaca tcgttggact
     1561 tatcttgttt aggtctcaga tctctaccac gattgcctga caatcttgat gaaattaatg
     1621 taagcaataa ccaactatca atgctccccg agctaccaag ggcattgaaa gagctgaatg
     1681 caagcagtaa tcaattatct gcacttcctg aattaccagt gtcgctggaa tatataaatg
     1741 tgagtgataa ccatttgttc gcacttcctg aattacctgc gtcactagaa tatattaatg
     1801 taagtgacaa tcacctgtct gtacttccga ggttaccaat gtcattggaa ttacttgatg
     1861 cagccagaaa tgctttggaa gtaataccag agtttccaga aagagatgat catattataa
     1921 gaatattctg gcttaatcag aaccggatca cggcaattcc ggaaagcata cttggcctca
     1981 gttctgatag cgttgtcaat cttagagaaa atcaactatc tcccagaata atgcaaactt
     2041 tgtt
//
LOCUS       DADWTH010000260         2032 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.266, whole genome shotgun sequence.
ACCESSION   DADWTH010000260 DADWTH010000000
VERSION     DADWTH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.266"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..362)
                     /locus_tag="JNY02_004268"
     CDS             complement(<1..362)
                     /locus_tag="JNY02_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9435518.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JNY02_004269"
     CDS             complement(429..1643)
                     /locus_tag="JNY02_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBB9435519.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVMMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          492 c          412 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcat gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgactactac catacaagag agaaaaagcg attattcaac cgtgttcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DADWTH010000261         2028 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.267, whole genome shotgun sequence.
ACCESSION   DADWTH010000261 DADWTH010000000
VERSION     DADWTH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.267"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            269..406
                     /locus_tag="JNY02_004270"
     CDS             269..406
                     /locus_tag="JNY02_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase mbeA domain protein"
                     /protein_id="HBB9435520.1"
                     /translation="MEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQGKEWVHLK
                     E"
     gene            413..589
                     /locus_tag="JNY02_004271"
     CDS             413..589
                     /locus_tag="JNY02_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBB9435521.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQV"
     gene            complement(948..1415)
                     /locus_tag="JNY02_004272"
     CDS             complement(948..1415)
                     /locus_tag="JNY02_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435522.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
BASE COUNT          525 a          407 c          581 g          515 t
ORIGIN      
        1 caaggctgtg acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc
       61 gacagaggaa tcactgaaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
      121 gaaggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcatttt
      181 cctgtttacc gtgagtggcg ggacgctctg gtatcttggc gggatgatac aggcgaacct
      241 ggaagaaatc aggaagcaga acgagagtct ggagaagctg aacgcgaaga catggggcgt
      301 ggagtttgtg caggacggga acaggaaatt tctggtcctt ccttacggga agtcagcaga
      361 ggtgatttcc tttcagggga aggaatgggt acatctgaag gagtgacaca gagtgacaga
      421 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
      481 gagcgaatgg gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga
      541 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgtagc acttgagcga
      601 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
      661 atacaggagc gcgaacatgc gttatccctg gaacgggagc gtcagccgga aatacaggaa
      721 cgcacgctgg atggtccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg
      781 attaggaggg gttaatgtgg cctgtaccat ctggttgcat aggtattcat gcggttaaaa
      841 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgtcattttt atctgtctgc
      901 tgccgtgatc gcaccaaacg cgtttcagtg gtgcgggcaa taagatatta cgttaaattg
      961 tgagagaatg gtccccatat actgacttta tcatacggat agggttggta gtgggtcgat
     1021 tgggtttcgg cattgtttcc gttaatgcca attgcatttt tgtatttgat gcaacagaag
     1081 gcgtgagatt ctcctgagcc tggaaagtct aagtgtttag aattgatggc aaaatatctt
     1141 ccgtccgatt cttgaagtag ttgttgtttg gctgtctccc aaagggtgct gttacaaact
     1201 tcttgaattt gtagttgttg caggactctt attactgcgt tgtcatgttc caattcttta
     1261 ggggagtgta ttattctctc ttttaataag ctgtttacca caggatttag ttgttgtcta
     1321 aagcacgcag ataatgccat tgctacacaa ttttgttccc gaggaagcat gtttggcggg
     1381 gtatgccgtg tttgatgtga tatgtctatt ctcatagaag gtctctggct tatagcatca
     1441 acaaatacgg ctataaaaca aaattttcat agagtgattc aagatcttct tgagatcttt
     1501 tttggcggtg cgtaatctgc tgcctgcaaa caaaaaaacc accgttatca acggtggttt
     1561 gtttgccgga atctagagct accaactctt tcttccgaag gtaactggct tggtggagca
     1621 cagataccaa atgctgtcct tctagtgtag ccgcagttag gccaccactt caagaactct
     1681 taatatctca atccacctta tccagttacc aatggatgct gccagtggcg ttaagtcgtg
     1741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     1861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcg
//
LOCUS       DADWTH010000262         1985 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.268, whole genome shotgun sequence.
ACCESSION   DADWTH010000262 DADWTH010000000
VERSION     DADWTH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.268"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..557)
                     /locus_tag="JNY02_004273"
     CDS             complement(<1..557)
                     /locus_tag="JNY02_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBB9435523.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
     gene            complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JNY02_004274"
     CDS             complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JNY02_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HBB9435524.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1321..1451)
                     /locus_tag="JNY02_004275"
                     /pseudo
     CDS             complement(1321..1451)
                     /locus_tag="JNY02_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1449..1544)
                     /locus_tag="JNY02_004276"
                     /pseudo
     CDS             complement(1449..1544)
                     /locus_tag="JNY02_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          565 a          339 c          420 g          661 t
ORIGIN      
        1 atgctgtcta atctgtttcc agaaactacc agtaatttta gttccggtgg caatagtggc
       61 agaatatgca atcggttatt atgaacccgt aaatgagtta gattttcagg aagaaatggt
      121 aattctttta tcttgttatc acccgcatta agctcttcta acgtctcggg taaggcaggt
      181 aagctttcca gttgattaaa ttgagcatgt attaccttaa gcattggtgg taaatcaggg
      241 agatgtgtta ataggttttt tcttatttcc agtgttgtta tctgaggcgg taaatctggt
      301 aatgttttta aatctaactc agataaattt aattctgcct cttgattttg taaacatatt
      361 ttcagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattcg
      421 ctccagactt ttaaataaaa ttcattacaa gatggagtca gttccatagg taaagataat
      481 ggagttaacg agtatatatt gtttatttga tgcataatgc cagaaccgat tgcctgtata
      541 ttggttgatt taatcattat aaccagaatt cttatcaacg ataagaattc tagtgaaata
      601 gaatatggct tattaatttt aaaacagttt gctttgtacc ctttctccgg gtattgcttt
      661 ccccctaagc cgtctatggg aaggagtctg ctgttattcc tgagaatggt aattcagcca
      721 taaaaaggta tttaaggtca ttgcgtctct ccctatactc gaataatggt gaagaaagca
      781 tgatgaatat atcacgataa cgttcagtac gtatcgagga tagatggagt tactcctctc
      841 tgaaatagaa ttactgctct atttttttct gttttcagaa gttattagta acaaaccctg
      901 atatattttt ttgaaaatgg agcttaaacg agtctgaaat taacttcaga aatcagaata
      961 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
     1021 tgtagcagtg aatttcaacg ttttcgtccg gctggcagca ggtatttgta attcgtatat
     1081 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
     1141 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
     1201 ttgtgtaagc atattacttc cttatgttat attttggtaa aaaatttatt tttttacatt
     1261 atagtcggct aaatgaagtt tactgtaaaa aaacgatact ttttcgggtg gcgcttccaa
     1321 tcagtagaat atgtgttttt caatgaagga gccgatgact tttcgtggat ttccacggaa
     1381 cgcgagaagc agattgtttt acgtgccagc cgcttgatgc gggtgcggag ggtcaggttg
     1441 ttacgttcaa gatatagcat gtgctggatg aagatccctt ttctggtcac ctgtttatct
     1501 ttcgtggccg acggggtgac actgttaaaa tgtaatgaac gcatcccaca caattaaaca
     1561 cggagtagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagctttccg
     1621 gcatcatact gcccaatacg ggatgcataa agcgtcctca gactggagac tccacaaatg
     1681 aaatatacac cggttggcgt tgatatggca aaacatgtca ttcagattca cttcatcaat
     1741 gagcacatgt cagcgccagt gatataagac ggtaatgggg atgcggacga tttcctggac
     1801 tctcagaaga tgaaacatgt ttactgaaga acaaaaaatt cgcgctattg agctttattt
     1861 caaatatggt aagaagcttg aggtgtactg gcaatagcgg acaccaccat ttgttctttt
     1921 tctaagcagt catctgataa tattttatcc tgaaggctgc cggggagata ttccccagac
     1981 gagag
//
LOCUS       DADWTH010000263         1977 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.269, whole genome shotgun sequence.
ACCESSION   DADWTH010000263 DADWTH010000000
VERSION     DADWTH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.269"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNY02_004277"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNY02_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBB9435525.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1977
                     /locus_tag="JNY02_004278"
     CDS             1494..>1977
                     /locus_tag="JNY02_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB9435526.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     V"
BASE COUNT          563 a          341 c          477 g          596 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat caggtct
//
LOCUS       DADWTH010000264         1931 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.270, whole genome shotgun sequence.
ACCESSION   DADWTH010000264 DADWTH010000000
VERSION     DADWTH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.270"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(854..1327)
                     /locus_tag="JNY02_004279"
                     /pseudo
     CDS             complement(854..1327)
                     /locus_tag="JNY02_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1255..1621)
                     /locus_tag="JNY02_004280"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="JNY02_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1931)
                     /locus_tag="JNY02_004281"
                     /pseudo
     CDS             complement(1623..1931)
                     /locus_tag="JNY02_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          428 a          538 c          465 g          500 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctacttgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacttgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccgaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgc atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggt gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tgacggggca
     1921 atatgcagtt c
//
LOCUS       DADWTH010000265         1902 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.271, whole genome shotgun sequence.
ACCESSION   DADWTH010000265 DADWTH010000000
VERSION     DADWTH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.271"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(569..781)
                     /locus_tag="JNY02_004282"
     CDS             complement(569..781)
                     /locus_tag="JNY02_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBB9435527.1"
                     /translation="MVVWRFGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINL
                     LDKSDYVLAHGLNTDDQLAVGIVYQF"
     gene            841..1902
                     /locus_tag="JNY02_004283"
                     /pseudo
     CDS             841..1902
                     /locus_tag="JNY02_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          494 a          395 c          533 g          480 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc cccgaatctc cagacaacca
      781 atatcactta tttaagtgat agtcttaata ctagttttta gactagtcat tggagagcag
      841 atgattgatg tcttaggacc ggagaaacgc agacggcgta ctacacagga aaagatcgct
      901 attgttcagc agagttttga accgggaatg acggtctccc ttgttgcccg gcaacacggt
      961 gtggcagcca gccagttatt tctctggcgc aagcaatacc aggagggaag tcttactgct
     1021 gtggccgcag gagaacaggt cgttcctgcc tctgaacttg ctgctgccat gaagcagatt
     1081 aaagaactcc agcgcctgct cggcaaaaaa acgatggaaa atgaactcct taaagaagcc
     1141 gttgaatatg ggcgtgcaaa aaagtggata gcgcacgcgc ccttattgcc cggggatggg
     1201 gagtaagctt agtcagctgt tgtctccggg tgtcgcgtgc gcagttgcac gtcattctca
     1261 gacgaaccga tgactggaaa gatggtcgcc gcagccgtca ctcagatgat acggatgtgc
     1321 ttctccgtat acaccatgtt atcggagagc tgcccacata tggttatcgt cgggtatggg
     1381 cgctgcttcg cagacaggcc gaacttgatg gtatgcctgc gatcaatgcc aaacgtattt
     1441 accggatcat gcgccagaat gcgctgttgc ttgagcgaaa acccgctgta ccgccatcga
     1501 aacgggcaca taccggcaga gtggctgtga aagaaagtaa tcagcgatgg tgctctgacg
     1561 ggttcgagtt ccgctgtgat aacggagaaa aactgcgagt cacgttcgcg ctggactgct
     1621 gtgaccgtga ggcactgcac tgggcggtca ctacgggcgg cttcaacagt gaaacagtac
     1681 aggacatcat gctgggagcg gtggaacgcc gcttcggcaa cgagcttccg gcgtctccag
     1741 tagagtggct gacggataat ggttcatgct accgggctaa tgaaacacgg cagtttgccc
     1801 ggatgttggg gcttgaaccg aagagcacgg cggtgcggag tccggagagt aacggcatag
     1861 cagagagctt cgtgaaaacg ataaagcgtg actacatcag tg
//
LOCUS       DADWTH010000266         1870 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.272, whole genome shotgun sequence.
ACCESSION   DADWTH010000266 DADWTH010000000
VERSION     DADWTH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.272"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            28..1870
                     /gene="cyoB"
                     /locus_tag="JNY02_004284"
                     /pseudo
     CDS             28..1870
                     /gene="cyoB"
                     /locus_tag="JNY02_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          356 a          488 c          504 g          522 t
ORIGIN      
        1 cattaaaggg gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg
       61 ttccatgaac ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc
      121 gttggcctga tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc
      181 gtcgaccata aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt
      241 ggttttgctg acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggtgaagcg
      301 ggcttcctgc cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc
      361 ttcttcgtgg cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc
      421 ggcgcgcgtg acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt
      481 ggcgtgattc tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg
      541 gcctatccac cgctatcggg aatagagtac agtccgggag tcggtgttga ttactggata
      601 tggcgtctcc agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc
      661 attctgaaga tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctaggca
      721 tcactgtgca cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg
      781 ttgttgaccc tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac
      841 atgatgatgt acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc
      901 ctgcctgttt tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt
      961 ggttatacct cgctggtatg ggcaaccgtc tgcatcaccg tgctgtcgtt catcgtttgg
     1021 ctgcaccact tctttacgat gggggcgggc gcgaacgtaa acgccttctt tggtatcacc
     1081 acaatgatta tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat
     1141 cagggccgca tcgtgttcca ttctgcgatg ctgtggacta tcggttttat cgtcaccttc
     1201 tcggtgggcg gtatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat
     1261 aacagcctgt tcctgattgc gcacttccat aacgtgatca tcggcggcgt ggtcttcggc
     1321 tgcttcgcag ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaacgaaacc
     1381 tggggtaaac gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg
     1441 tatgcgctgg gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc
     1501 cacaccatgc tgatgattgc agccagcggt gcagtactga ttgcgctggg tattctctgc
     1561 ctcgttattc agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc
     1621 gacccgtggg gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac
     1681 tttgccgtag tgccgcacgt tcacgaacgt gatgcattct gggaaatgaa agagaaaggc
     1741 gaagcgtata aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca
     1801 ggtatcgtca ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg
     1861 tggctggcga
//
LOCUS       DADWTH010000267         1828 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.273, whole genome shotgun sequence.
ACCESSION   DADWTH010000267 DADWTH010000000
VERSION     DADWTH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.273"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..168
                     /locus_tag="JNY02_004285"
     CDS             <1..168
                     /locus_tag="JNY02_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBB9435528.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="JNY02_004286"
     CDS             153..791
                     /locus_tag="JNY02_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBB9435529.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1410
                     /locus_tag="JNY02_004287"
                     /pseudo
     CDS             1019..1410
                     /locus_tag="JNY02_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1429..1800
                     /locus_tag="JNY02_004288"
                     /pseudo
     CDS             1429..1800
                     /locus_tag="JNY02_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          544 a          324 c          435 g          525 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagtgggggg ggagatgaac cctatgcaat gtcctggtta
     1441 atatctcagt gtgctcatca gtgcactgat aataaaaaga cggagacaga tgccatttac
     1501 gataaagttc ggagttctta tttgctttca tgtattctga aaaaaaacaa aaatgttggg
     1561 cttattcttc atgcaccatc atttgtttct gtgtctgaaa aaatcgccag aattgtgatg
     1621 gcaaattact cccgtaactg gagtaattcc gagttggcca gtgcggtttt aatgagtgaa
     1681 tcttctttaa aaagaagaat gtacaaagag gtcggttcta taagtacatt tgtacataaa
     1741 ataaagctta cagaagctat caggaaatta cgaaggacaa atactcccat ttcggtaatg
     1801 ctgccaactt actgatttag tgtatgat
//
LOCUS       DADWTH010000268         1826 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.274, whole genome shotgun sequence.
ACCESSION   DADWTH010000268 DADWTH010000000
VERSION     DADWTH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.274"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(39..308)
                     /locus_tag="JNY02_004289"
                     /pseudo
     CDS             complement(39..308)
                     /locus_tag="JNY02_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(423..1061)
                     /gene="ospI"
                     /locus_tag="JNY02_004290"
     CDS             complement(423..1061)
                     /gene="ospI"
                     /locus_tag="JNY02_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HBB9435530.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1220..1690)
                     /locus_tag="JNY02_004291"
                     /pseudo
     CDS             complement(1220..1690)
                     /locus_tag="JNY02_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          515 a          452 c          337 g          522 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggagccagtc tacccagcaa gcgctgtaac
       61 tcttttattt gttttatagc ggcagccagt tctgaggcag gaacgacatc ctctccggca
      121 gcaacagcag tgagacttcc ttccagatat tattttttcc acttaagcaa caggctgggc
      181 tgaataccat gcaaacgcgc cacatgggac accgtcatgc caggctccat tgtctgctga
      241 ataatggcaa ttttttcctg aggagtttta cgtttacgaa gctccagccc cagcaaagtt
      301 ccagtcatct caaaattaga actagtgtta gacatatatc caagcctatc tcttatttta
      361 agatacatcc actgtctggc gtttcaaggg gccggctcaa atctatgtac aggctctcag
      421 catcagcaaa gcctcttact tttccaacta ctactgttag catagtgatc aaattcctga
      481 tcagatacat gaattacctg aaccgaacta ttttcatcta caaatgcatc tggcgatttt
      541 tctacaggat cataaagatt aaatcccttt cctgtttgaa catccaaaaa cataattttt
      601 tcaccatcgt taaatccagc tatccaatga gtggcctgtt caatagaaat cattactaca
      661 cttccttttg aagtattttg taaaatattt tctgcaatac gctcacgaaa tttactataa
      721 gtttcattag gatgtgacac tatgccttca ctatcaattt gctctaaatg cctatgaaaa
      781 aatccggtca tatactctga tatttcatta ggtacgggag cctctcttac tccataccct
      841 aacatagcca tacaagcatg aagcgcacac cctgaacagt taccatcccc accagacgga
      901 tttatcattt tctgtaaagt cgctgccaat tcagaatctt tatccatatc accaagactt
      961 ttagagttta aagattccag caatttttta acatttacat ttccctcctc tgtatgtgcc
     1021 tcatatccag cagttccctg taacgacacc ccattaatca tattgtatcc tgacagtata
     1081 tatactcact atattttctt cataacaaat atccctctga tatgacaaca aaaaacggag
     1141 ccggattcct gtttttgtga actgcatcta ttcgtgtcta aaaagtgatc cacttaccac
     1201 caacggccta atatatacag aacggagtaa acagggctaa cagttcccga caggtctcat
     1261 cattccgtgg cccgaaagtg taagccaaca caccgccagt tttagtgttg tacgcatacc
     1321 agagccagtg ttgttgcagc cttgctgcca acgaagcgcc actgctcatc aagttcacag
     1381 ataagggcaa catcagcgtg agcgactggc gcagatgtta tccgccgggg cgggagcttt
     1441 ttaatgtccg tatgacggta ttgatgccga ttttcagtgt cctggcggta tcacggcccc
     1501 ctgcgccgtt atgtgccatt tcaacgatct gctccttaac gccgggtttt ctggcttcat
     1561 aagagtaagt gagctggaaa acacgtttac atgagcgaca acggaaacgc tcatgccggg
     1621 aacaactacg gccatgtcga taaacttcat cggaatgaca gcgcggacaa tgaacagtaa
     1681 cggtagccat ggaaaacctt aaataccagt agattaccag aaacttctgt cgattgaaga
     1741 catcacccga agtttgaatt cattcgaata ctttttacga ggttcttttc gccagtcctg
     1801 taattcgtaa gcgccccatc agcgac
//
LOCUS       DADWTH010000269         1782 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.276, whole genome shotgun sequence.
ACCESSION   DADWTH010000269 DADWTH010000000
VERSION     DADWTH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.276"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..452
                     /locus_tag="JNY02_004292"
     CDS             <1..452
                     /locus_tag="JNY02_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435531.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGRWYNAGSI"
     gene            455..988
                     /locus_tag="JNY02_004293"
     CDS             455..988
                     /locus_tag="JNY02_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBB9435532.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="JNY02_004294"
     CDS             complement(1017..1544)
                     /locus_tag="JNY02_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBB9435533.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1782)
                     /locus_tag="JNY02_004295"
                     /pseudo
     CDS             complement(1546..1782)
                     /locus_tag="JNY02_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          499 a          391 c          399 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggaggtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcaaag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cg
//
LOCUS       DADWTH010000270         1773 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.277, whole genome shotgun sequence.
ACCESSION   DADWTH010000270 DADWTH010000000
VERSION     DADWTH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.277"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..284
                     /locus_tag="JNY02_004296"
     CDS             <1..284
                     /locus_tag="JNY02_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435534.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="JNY02_004297"
                     /pseudo
     CDS             286..890
                     /locus_tag="JNY02_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1773
                     /locus_tag="JNY02_004298"
     CDS             877..>1773
                     /locus_tag="JNY02_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435535.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLPDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          426 a          403 c          496 g          448 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggaaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 ccagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttc
//
LOCUS       DADWTH010000271         1764 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.278, whole genome shotgun sequence.
ACCESSION   DADWTH010000271 DADWTH010000000
VERSION     DADWTH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.278"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(417..608)
                     /locus_tag="JNY02_004299"
     CDS             complement(417..608)
                     /locus_tag="JNY02_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBB9435536.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            673..996
                     /locus_tag="JNY02_004300"
     CDS             673..996
                     /locus_tag="JNY02_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBB9435537.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            986..>1764
                     /locus_tag="JNY02_004301"
     CDS             986..>1764
                     /locus_tag="JNY02_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB9435538.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELTPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGIDVVRVTRSSISIADPN
                     GGKNVRLKGAFYEQSFTDGCGVREKAERESRIYRENAERRV"
BASE COUNT          398 a          447 c          544 g          375 t
ORIGIN      
        1 aacgccagcg gcgcagcctt tctttgccct ttgccttttt gctcacatgt tctttccggc
       61 cttatcccct gattctgtgg atcaccgtat taccgtcttt gagtgcgctg ataccgctcg
      121 ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaag agcgccggaa
      181 gctgcatttt ctccttacgc atctgtgcgg catttcacat cggctatggt gcacttttca
      241 tacaatccgc actgatgccg cataagtaag cctgtgtaca ctccgctatc gctacgtgac
      301 tgggtcatgg ctgcgccccg acacccgcta aaacctgctg acgcgccctg acgggcttgt
      361 ctgctcccgg catccgctca cagacaagct gtgaccgtct ccgggagctg catatgtcag
      421 aggttttcac cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc atcggcgtgg
      481 tcgtgaagcg attcacagat atcagcctgt tcatccgcgt ccagttcgtt gagcttctcc
      541 agcagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
      601 ttagtcactg atgcctccgt gtaaggggga tttctgtttt atggggtaat gataccgatg
      661 aatcgcgaga ggatgctcac catacgggtt actgatgatg aacatgcccg gttactggaa
      721 cgctgtgagg gtaaacagct ggcggtatgg atgcggcggg tctgcctggg tgagccggtt
      781 gcccgttccg ggaaactgcc gacactggca ccgccgttac tgcgtcagct ggctgccatc
      841 gggaataacc tgaatcagac agcgaggaag gtgaacagcg ggcagtggtc ttccggtaac
      901 cgggttcagg tggtggccgc actgatggcc atcggggacg agctgcgccg gctgcgtctg
      961 gccgtcaggg aacaggggac gcgggatgat agttaagttt catgcccggg gaaaaggtgg
     1021 tggcagtggt ccggttgatt acctgctggg aagggagcgt aaccgggaag gggcaacggt
     1081 gcttcagggt aatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa
     1141 atacacgtcc ggtgttctgt cgttcgcgga gaaggagctg acgccgggag gacgtgaaaa
     1201 agtgatggcg agctttgagc gtgtactgat gcccggtctc gaaaaaaatc agtacagcat
     1261 cctgtgggtg gagcaccagg acaagggacg gcttgagctg aattttgtca ttccgaacat
     1321 ggagctacag accggaaaac gcctccagcc gtactacgac cgcgcagaca ggcctagaat
     1381 tgatgcctgg cagacgctgg ttaatcacca ttacgggctg catgacccga acgccccgga
     1441 gaaccgcagg acgctgacac tccctgataa tctgccggaa acgaaacagg cgcttgcaga
     1501 gagcgtcaca cgcgggatag acgctcttta tcatgtcggg gagataaaag gccgtcagga
     1561 tgtgattcag gcgctcacag aggcagggat tgacgtggtc agggtgacgc gaagcagtat
     1621 cagcattgca gacccgaacg gcgggaagaa tgtcagactg aaaggagcgt tttatgagca
     1681 atcttttaca gacgggtgcg gagttcgaga aaaagctgaa agagagagcc gaatctacag
     1741 agaaaatgct gaacgacgag ttca
//
LOCUS       DADWTH010000272         1746 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.279, whole genome shotgun sequence.
ACCESSION   DADWTH010000272 DADWTH010000000
VERSION     DADWTH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.279"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            44..976
                     /locus_tag="JNY02_004302"
     CDS             44..976
                     /locus_tag="JNY02_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBB9435539.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1052..1126
                     /locus_tag="JNY02_004303"
     tRNA            1052..1126
                     /locus_tag="JNY02_004303"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Arg,seq:cct)
     gene            1288..>1746
                     /gene="intS"
                     /locus_tag="JNY02_004304"
     CDS             1288..>1746
                     /gene="intS"
                     /locus_tag="JNY02_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBB9435540.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT          492 a          398 c          438 g          418 t
ORIGIN      
        1 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
       61 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
      121 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
      181 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
      241 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgtcg aactggaagg
      301 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
      361 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
      421 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
      481 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
      541 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
      601 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
      661 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
      721 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
      781 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
      841 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
      901 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
      961 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
     1021 agtccattag cgggtatact catgccgcat tgtcctctta gttaaatgga tataacgagc
     1081 ccctcctaag ggctaattgc aggttcgatt cctgcagggg acaccattta tcagttcgct
     1141 cccatccgta ccagtccgca aaatcccctg aatatcaagc cttccgtata ttcacagttc
     1201 gtcatggttc gcgtcagatc gttgacagcc gcactccatg acgggtaaaa agtggataaa
     1261 ataattttac ccaccggatt tttacccatg ctcaccgtta agcagattga agcagcaaag
     1321 ccgaaagaaa aaccataccg ccttctcgat ggtaatggcc tgtaccttta tgtccctgta
     1381 tccgggaaaa aggtatggca gcttcgctac aagattgacg gtaaggagaa aatcctgacc
     1441 gtcggaaaat atccgcttat gactttgcag gaggcaaggg ataaagcatg gactgcgagg
     1501 aaagacatct cggctggcat cgatccggta aaggcgaaaa aggcttcgtc taacaacaat
     1561 tcatttagtg cgatttacaa ggaatggtac gaacacaaga agcaagtctg gtcagtaggg
     1621 tatgcgactg aacttgcaaa aatgtttgat gacgacattt tacccatcat cggcggcctt
     1681 gagattcagg atattgagcc gatgcaactg ctggaagtaa tccgcagatt tgaagatcgc
     1741 ggtgca
//
LOCUS       DADWTH010000273         1735 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.280, whole genome shotgun sequence.
ACCESSION   DADWTH010000273 DADWTH010000000
VERSION     DADWTH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.280"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..166
                     /locus_tag="JNY02_004305"
     CDS             71..166
                     /locus_tag="JNY02_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBB9435541.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="JNY02_004306"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="JNY02_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBB9435542.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            974..1144
                     /locus_tag="JNY02_004307"
     CDS             974..1144
                     /locus_tag="JNY02_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBB9435543.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
BASE COUNT          642 a          288 c          278 g          527 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc gtcgt
//
LOCUS       DADWTH010000274         1731 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.281, whole genome shotgun sequence.
ACCESSION   DADWTH010000274 DADWTH010000000
VERSION     DADWTH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.281"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(219..773)
                     /locus_tag="JNY02_004308"
     CDS             complement(219..773)
                     /locus_tag="JNY02_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBB9435544.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(770..1060)
                     /locus_tag="JNY02_004309"
     CDS             complement(770..1060)
                     /locus_tag="JNY02_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBB9435545.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1060..1659)
                     /locus_tag="JNY02_004310"
     CDS             complement(1060..1659)
                     /locus_tag="JNY02_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBB9435546.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          415 a          477 c          394 g          445 t
ORIGIN      
        1 ggtggcgaac tgtgcagggt tggcgtaacc gggtacggaa accggcgagc ctttcggctc
       61 ccccacacag cccgccataa atcgcgaatg tgactgtgca aacgatatga aaaaagacgc
      121 gggcgcgtct catatcgctc cgtaaacatc cgggacgcca atcccgacgc cagattttgc
      181 tggcgcgtga ggaatatagc cccggataac agattgagtc aacaaacggt ttttagatcc
      241 ccggaagaga atgcatcacg catcggtaga tagagcatat attcagcaag atttagccat
      301 acatctaccc gactgcagca cgtccggaaa caccactcag ggtgtgcgtc attcagcaat
      361 tcagccattt tacgcttagt catcccccgc ccttcatacc gttgccggag gacactaatc
      421 aatccaggat gctctgccag cacctcactt atgactctat caatacataa cgcctctgca
      481 tcagtacaat gcgccagcca ggtcttttgc ttgccattga tcatctctcg caaaaacgct
      541 tccagctcag gtttctctat tcccgctttt ttcatcctgc gcagggcttc attgacggct
      601 gttttcgtca attttttgga tgccagcaac tggttaaaca tatttcccgt cttaccgccg
      661 ccaatatacg accagcgccc ccacatgcgc agttttccct gaatccagac actttccagc
      721 gtggtgagac gaaggtgttc cccgcttttg cctgtatttg ttgggtaaat cataaataac
      781 cttcctttct ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt
      841 cttctttggt gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg catgagctac
      901 aggcaatcgc tgcctgcata tcgtgtggtt ttgtcgctgt tccgcacgtc cccgccagcc
      961 tgtaatgcgc cagcacagaa gtttcgggat tgtgattgca gtagccagga attctgacgg
     1021 tgcacatctg cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagtaactg
     1081 cgcggccaca ttttcgactt cctccggaga ggaaaattta cggaacagga tccagttcca
     1141 cagcacattc agtacagatt tataaacctg ctgaaactcg gtttcgtcca tgttcgcaaa
     1201 tgcgatagat tttgccctgc gcccacgact accatcagga taaatatgct cggtgtaaaa
     1261 tccggcctga atggttaccc actcgcggaa agcgtcaaac gactttagca atgccgtatc
     1321 ccgggttctg catgtcgcaa ctgtattaag gtattgctct gcggcatcac tcagggctgg
     1381 agtgtgttcc cgacctactg attcgcacag gtaatcaacg aagcctgata ccagttttcg
     1441 ttcgcgaggc gtgatcgccc caccgaccgg agtccagtaa tcaaatccca gttgcaggag
     1501 tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca
     1561 ctcacctatt ttgatttgat gcaaaaaatc gcaactctcc ggcgtcgccg ggagaagtaa
     1621 tccggaagag gtttgtttga ccagttgtat atgcgccatc gtagttctcc gctggcgcag
     1681 tagaatgggt gttcagcccg ttatgtagta taccagaatt aatgccaata c
//
LOCUS       DADWTH010000275         1716 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.282, whole genome shotgun sequence.
ACCESSION   DADWTH010000275 DADWTH010000000
VERSION     DADWTH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.282"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(475..723)
                     /locus_tag="JNY02_004311"
     CDS             complement(475..723)
                     /locus_tag="JNY02_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435547.1"
                     /translation="MITDTSKLYDDDDELWIKNFLEYIEHDDGVAAKESLMKGVSIYY
                     AEPDTPENCVIKEYPDGRKELVSFMTGKEIPVPPRFRS"
     gene            1470..>1716
                     /locus_tag="JNY02_004312"
     CDS             1470..>1716
                     /locus_tag="JNY02_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435548.1"
                     /translation="MRRITQVDQGTGEELGGFVAVIRPKQKSAFQRHFTMNQAALITI
                     ANELNHDQMRVLMALLAELDYENYIQVAQMDIAEALSM"
BASE COUNT          426 a          435 c          436 g          419 t
ORIGIN      
        1 aaaaaacaaa tgtcagccgt gcggtaaaaa acctgatcga gttcgaaatt atattggaag
       61 ggccgaagat cgggcggagc aaaacatatc gtctaaaccc tcagttcggc tggaaaggca
      121 cggtaagcaa ccacaagaaa gctctcaaaa acggacttag tgttattcag ggtgggagag
      181 tttaacgccc cgttccctgc ctgtgtgctg gcggtctttc tccgtcttat ccctgaactg
      241 gttattttcg gatgcagctg tgtgtggcgg tactccaaaa acacatcttg tctggtacac
      301 agatttcctt tggcattcct tcgagtttcc ctgagatttc cactctgcat gtacacacgt
      361 atacacgcat aaccagcggc aaagttaggg caggacatgt aaagttagcc aactttacgc
      421 ctgccttatt cgcgtcccgc tcaaaggaaa aaccccgttc tataggttga atcgtcaact
      481 tctaaatcgg gggggtactg gtatttcttt tcccgtcatg aaacttacca gttctttacg
      541 cccgtcagga tattctttga taacacaatt ttctggggtg tcaggttctg cgtagtagat
      601 ggatacgcct ttcataagac tttctttcgc tgctacccca tcatcatgtt caatgtattc
      661 cagaaagttt ttaatccata attcatcatc atcgtcgtac agtttagatg tatcagtaat
      721 catttggttt cctcggcttt tggcgataca tgacctgcgt gatttggagt gtgcactcta
      781 gactttgcta tgcaaagtcg ttcgagcagc aagctgccat aacggaacgg ttccggtagc
      841 gccttgagcg ctgccggaac cgttcggggg cacgccccca accccccgca tcgctgtaac
      901 accgacggtc tacgaccacc caccgaccgc ggggcggcca gtcccgtgcg gttaggctta
      961 gcaggggcgc ggggcgctgt cccgtagttc cggtaacgac cgtaacccgc tggcgcgggg
     1021 cttcggcgcg ctaggcctcc tgagagcctc tgtaagcgtt tttcgcgcct tccatgccct
     1081 aacgggcatt taaaacctgt ttcgtgcgtc tgagggcttc tgagcgcgtc tgaggggcta
     1141 tctggcgacc ctccccgaaa gaacaacaac ggaatcgccg acaacacgag agagctgaca
     1201 ccgctcgccg cagccccagc gaccgaacgt agtgagcgag cgggcgagga agcggaattt
     1261 ccacttcccg aacatagaaa tccttacgct cggaactggc agaaaaactc ctgatttttc
     1321 gaatgtgcga acgttgggga gtaataagat acagaagggg gtgtctcgga agccgcgcca
     1381 caagcggaaa gtttttgaca atagttatct ctagagataa cttagttatc ttgagagata
     1441 actatttttc ctgaaatgga gatttttaga tgcggagaat cacacaggta gaccagggta
     1501 ccggggagga gttaggcggt tttgtagcag tgattcgacc caaacaaaag tcagcatttc
     1561 agaggcactt taccatgaac caagccgcac taattaccat tgccaatgag ctaaaccatg
     1621 accagatgcg tgtgctgatg gccttgctcg cagagctaga ttacgagaac tacatccagg
     1681 tagcacaaat ggacattgca gaggcgctga gcatgc
//
LOCUS       DADWTH010000276         1679 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.284, whole genome shotgun sequence.
ACCESSION   DADWTH010000276 DADWTH010000000
VERSION     DADWTH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.284"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(72..539)
                     /locus_tag="JNY02_004313"
     CDS             complement(72..539)
                     /locus_tag="JNY02_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBB9435549.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFFALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="JNY02_004314"
     CDS             complement(605..844)
                     /locus_tag="JNY02_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBB9435550.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="JNY02_004315"
     CDS             complement(979..1584)
                     /locus_tag="JNY02_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB9435551.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          543 a          369 c          294 g          473 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc aaaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DADWTH010000277         1662 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.285, whole genome shotgun sequence.
ACCESSION   DADWTH010000277 DADWTH010000000
VERSION     DADWTH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.285"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..933)
                     /locus_tag="JNY02_004316"
                     /pseudo
     CDS             complement(1..933)
                     /locus_tag="JNY02_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            complement(961..>1662)
                     /locus_tag="JNY02_004317"
     CDS             complement(961..>1662)
                     /locus_tag="JNY02_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBB9435552.1"
                     /translation="ESWGINTTYVEKVPCTSSGVAPIFVNANSSNSILIIKGANKFLS
                     PEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYA
                     CKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQ
                     EVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGMQSS
                     YPSIEQFNEFLTLNE"
BASE COUNT          519 a          366 c          328 g          449 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagaccatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt ggaaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaatacctg acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aagtgtacaa ccaccaatat acagcgtcag
      661 gccaattaaa attgccacct tataactggt ctttttaatc acaagggatg ctggtattgc
      721 aattaaaaaa taacctccat aaaatgcgct ctgcaccaat gctgaagcaa agttacttag
      781 cgaaaataca cttttgaatt gagtgattaa tatgtcattt aatgcagctg cgcatcccca
      841 tagcgggaat aaacacgata acaaaataaa ctggaacaag ggagtcttat tcagataccc
      901 atcaggcatc tgaatgatgt ttttatcgtt catagtgcta cctttaactg tgcaggatga
      961 ttattcgttt aaggttaaaa attcattaaa ttgttcaata ctcggataag atgattgcat
     1021 acctttccct gtgacgctga aagcggcaaa gagagcggct tttttcaaag cggcttcaac
     1081 atcaccgctt tgaacataat aatgggaaaa gcaaccaata aatgcgtcac cagcgccact
     1141 agtatcaaca gcatttactt tgaatgcagg aacatggact tcctgatcgc gggtcatcca
     1201 taatgcgcct ttttcgctca tggtaacaat aatattgttc agccctttat caactaacga
     1261 acgtgcggcc aaacgaatat gatcataagt atcaaccgac ataccggtta atatttccag
     1321 ttctgtttca ttcgggataa agaaatcacc tttgcaggca taagacatat ctaactcacg
     1381 caatgccgga gccggattta ataacacttc aataccattt ttcttaccaa actcaatcgc
     1441 gtggtaaact gtttccagtt gaacttccag ttgtaaaacg atcaatttgc attttttcag
     1501 atcttctgca gctcgatcga tatcttccgg ggaaagaaat ttattcgctc ccttaattat
     1561 taatatacta ttgctcgagt tggcattaac aaagatcggt gcaacaccac tgctggtaca
     1621 ggggaccttc tcaacataag tggtattaat tccccatgat tc
//
LOCUS       DADWTH010000278         1622 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.286, whole genome shotgun sequence.
ACCESSION   DADWTH010000278 DADWTH010000000
VERSION     DADWTH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.286"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(147..1007)
                     /locus_tag="JNY02_004318"
     CDS             complement(147..1007)
                     /locus_tag="JNY02_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBB9435553.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1190..>1622)
                     /locus_tag="JNY02_004319"
     CDS             complement(1190..>1622)
                     /locus_tag="JNY02_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB9435554.1"
                     /translation="TDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQ
                     GVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRR
                     TVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT          417 a          415 c          354 g          436 t
ORIGIN      
        1 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
       61 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
      421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
      481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
      541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      601 agtaagttgg cagcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgca
      781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      961 gccgcaaaaa agggaataag ggcgacacga aaatgttgaa tactcatact cttccttttt
     1021 caatattatt gaagcattta ccagggttat tgtctcatga gcggatacat atttgaatgt
     1081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1141 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1201 tttcgtcttc aagaatttta taaaccgtgg agcgggcaat actgagctga tgagcaattt
     1261 ccgttgcacc agtgcccttc tgatgaagcg tcagcacgac gttcctgtcc acggtacgcc
     1321 tgcggccaaa tttgattcct ttcagctttg cttcctgtcg gccctcattc gtgcgttcta
     1381 ggatcctccg gcgttcagcc tgtgccacag ccgacaggat ggtgaccacc atttgcccca
     1441 tatcaccgtc ggtactgatc ccgtcatcaa tgaaccggac tgccacgccc tgagcgtcaa
     1501 attcctttat cagttggatc atatcggcag tgtcgcggcc aagacggtcg agcttcttaa
     1561 ccagaatgac atcaccttcc tccaccttca tcctcagcaa atccagccct tcccggtctg
     1621 tt
//
LOCUS       DADWTH010000279         1606 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.287, whole genome shotgun sequence.
ACCESSION   DADWTH010000279 DADWTH010000000
VERSION     DADWTH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.287"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>1606)
                     /locus_tag="JNY02_004320"
     CDS             complement(<1..>1606)
                     /locus_tag="JNY02_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBB9435555.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESG"
BASE COUNT          413 a          416 c          346 g          431 t
ORIGIN      
        1 atccgctctc actttctggt gtcgaaagtg aataattgaa gatgcatttt gttgaggcgt
       61 tttctcccca ggataacgta acctgtcctt tttctggtag accagaaaga taaagttcgc
      121 cacggtcacc gacaattcct gaatctgcca aacttgcatc ttgtatcgca gcaatggctc
      181 caaaaggtac cggtttacca tttgcattta acactctgaa gagaacgcga taacccacat
      241 gagtcacaaa ctccgcttta accactgcac cacgcgtggg aaccatcgta acgacggtat
      301 tatcaagttc tatattcgac ttcgagaaat agttatcgct gattgctacc cgattttcat
      361 cataagggtt tagctgtgtc ttgaccgtat agccgcgcca gtccgtcgca gcaccgcgca
      421 tattatctat ttctaacccg gcggccccag gtgctttaac aagagcaata gtttcaccta
      481 actcctgact taacgttacc ccttcgctgt gtgccagaat tccccctctg ataccataat
      541 tcagcctctg ggaattttgg ctgtagttat agctgccgtt caccgtgcca taagagccat
      601 cataggtcag ggacatatta ccgccaacct catcatcatg actgttatag gactgcatta
      661 aattccatga aaggttattt ctttccagcg ccgtaccgct gagtccgagt tgatgtgagc
      721 taccggaatt atcgttttgc gaaacagaat aggtggcgta actattaccc caccagcccg
      781 ataaaggaac agataaagag agcatgtaga tctgctcact ggtgttatca ctggcatggt
      841 tgttccccgt cccctgtctg gcagtattgg aataactcca ggagacgttc cacgagagac
      901 gcttccagct actgctgtag ccaaattgca ttaatcgttc gacatcatcg gtatgccagt
      961 attgttgccg gcttaatgtc aggtataaat tgccataatc tctccccaac gactgcgtta
     1021 gcgttccctc cacgcgacta cgtcggttac ctgtttccca aaagtcttca gggctgttgc
     1081 gccgcgatgc ccactcattg agggtatagt agccctcggt ggagtaacga tagccaacca
     1141 ggcgcaagct agtgtctgtg tcgtcaaata gttttgaata gagaaagcga tatgactgac
     1201 ccgtttctgt atcatcatgg ctaaaattcg cacgagcatg tgtcacatcg aaagaaattg
     1261 cgcccaaagc gccaagatct ttaccgacac ccagagctgc agaaccataa ttttcagcag
     1321 cctgcatacc tccatataaa gtgatcccgt gggaaaaacc atgaagtatt tgcgcctgaa
     1381 ggacgtccgg tgtaaatccg tcttcatcgc gcaattcacc cacgctgaca tcaacatctg
     1441 tcagaccttc acgtttaaga atcgccaatg aagcataagg ttgggtgaat cgttgttcgc
     1501 taccgtcttc ttctttgatg gttacgtcca aatcgccacc tgtacttgcg gtgttgagat
     1561 cggtgatctc aaaagcacct tgtggaacgg ctgactgata aatcac
//
LOCUS       DADWTH010000280         1587 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.288, whole genome shotgun sequence.
ACCESSION   DADWTH010000280 DADWTH010000000
VERSION     DADWTH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.288"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..217
                     /locus_tag="JNY02_004321"
     CDS             <1..217
                     /locus_tag="JNY02_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBB9435556.1"
                     /translation="AEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            425..694
                     /locus_tag="JNY02_004322"
     CDS             425..694
                     /locus_tag="JNY02_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435557.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            691..917
                     /locus_tag="JNY02_004323"
                     /pseudo
     CDS             691..917
                     /locus_tag="JNY02_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            946..1536
                     /gene="ospG"
                     /locus_tag="JNY02_004324"
     CDS             946..1536
                     /gene="ospG"
                     /locus_tag="JNY02_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HBB9435558.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          456 a          312 c          406 g          413 t
ORIGIN      
        1 ggcagaagag cagaaatatg agatgctgga gaatgagtac cctcagaggg tggctgaccg
       61 gctgaaagca tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt
      121 gatgcgtgag actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg
      181 attgtctgaa aacggctcac aactgcacca ttcataatca catcgcataa aactgtcagc
      241 gccagtgata ggacaggtga aacctgtcaa tacgtaccgg cttacgccgg gcttcggcgg
      301 ttttactcct ctgccatatg caacaacgga gtgccgcctt acatgccgct ggcgcggcat
      361 atcctggtga cgatatctga acagttatat actgtgtata tacgtggtaa tgacgaggtg
      421 gcaaatggca caggttaata tgagtgtaag aatcgacgca gaactgaagg atgcttttat
      481 ggccgctgca aaaagcatgg accgtaatgg ctctcagtta atccgggatt ttatgcgcca
      541 gaccgttgaa cggcagcaca attcttggtt ccgtgaccag gttgcggcag gccgtcagca
      601 actcgaatgc ggtgatgtgc ttccccatga catggtcgaa tcctctgctg ctgcatggcg
      661 ggatgaaatg agcaggaaga ttgctgataa atgatggaga tgttctggac catgctggcc
      721 atccaggaca ggaagcgcat tcgtagtaca ttgcggagca gaacctgatg gccgccattg
      781 aaccggacga acggattggt tattcagcca gcagcctggc agggcagccg tataaaggcc
      841 gcaacggtcg tgtggaagga acatctggac cacacaaggt agcgtgtaac gttattttgt
      901 gtgaaaatct cttgtgattt ttaaatatcg taacaaaggt atgacatgaa aataacatct
      961 accattattc aaacaccttt tccatttgag aataataatt ctcatgctgg catagtaacg
     1021 gagcccattc tcggtaagtt aataggtcag gggtcgacag cagaaatctt tgaagatgtg
     1081 aatgattcat ctgctttgta taaaaagtat gatcttattg gcaaccagta caatgagatt
     1141 ctggaaatgg cttggcaaga atctgagctt tttaatgctt tttatggcga tgaagcatcc
     1201 gttgttatac agtatggcgg agatgtgtac ctccgaatgc tgcgcgtgcc tgggactccc
     1261 cttagtgaca ttgatacagc tgatatccct gataatatag agagccttta tctacagttg
     1321 atatgtaaat tgaatgagtt gagtataatc cattacgatc ttaatacagg taatatgctg
     1381 tatgataaag aaagtgaaag tttattccca atagattttc gcaatattta tgctgaatat
     1441 tacgctgcaa ccaaaaaaga taaagagatt atcgaccgac gattacaaat gcgtacaaat
     1501 gatttttatt cgttattaaa caggaaatat ttatagccgt atttgttgat gctataagcc
     1561 agaggctctt ctatgagaat atgggga
//
LOCUS       DADWTH010000281         1417 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.289, whole genome shotgun sequence.
ACCESSION   DADWTH010000281 DADWTH010000000
VERSION     DADWTH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.289"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..210)
                     /locus_tag="JNY02_004325"
     CDS             complement(<1..210)
                     /locus_tag="JNY02_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435559.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPK"
     gene            537..839
                     /locus_tag="JNY02_004326"
     CDS             537..839
                     /locus_tag="JNY02_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBB9435560.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            830..1312
                     /locus_tag="JNY02_004327"
     CDS             830..1312
                     /locus_tag="JNY02_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB9435561.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDNARAFYERFGFVQSVVAPNTLFYKV
                     "
BASE COUNT          354 a          300 c          352 g          411 t
ORIGIN      
        1 cttcgggtca tccgccttca tctccgcttc tgtttcgtat aaatcaaaac gacggcaggt
       61 atggcagaac gtgacgtatt cgtacggtga atccgggtac attttgtgga aggtttccca
      121 gaaacagacc gtgcaggagg gatgaccggt gataaagtct gcaaatacgg acttataagg
      181 gtgattattg gctctggcga cggctttcag aaccctcttt caccattctg gtgtggactt
      241 tctggtgctc caggttgtgt gacatgggaa ctcattctgg atggttactc tgaaagctca
      301 tattctgcca caccccgatt tgcagccgcc aggctgccgt ggttcaggca tttcattatc
      361 aaaatcacat taaacggccg taatcagaca tgatttgtgc gccgacacag atcatcgtca
      421 caatgttcag gtctctgcca ttgtttttat caggggatcg cttttcccgt aagtgattct
      481 gtatagcgtt ctgttaccat ttgcgataac attaattgca tagcaaattg ttaccaatgg
      541 aggtttttat gtctacagct gcaagcgtcc gtaaaacacc gcgagaacat cagattaaca
      601 tccgggcaac tgatgaggaa cgagccgtta ttgattatgc tgcgagcttg gtcaataaga
      661 accgtaccga tttcatcatg gaactggcgt accaggaagc taaaaacatt atcctggatc
      721 aacgtttatt tgtgctggat aacgaacgtt atgattcgtt tattacgcaa ctggaagctc
      781 ctgtgcagaa tgcagaaggg cgtgaacgtc tgatggcggt aaagcctgaa tggaaataaa
      841 tgtcaccgcg ccagcattgt tgacggatga gcatatactt cagccatttg actgtggaaa
      901 tgaggtgcta agtaactggt tacgtggtcg ggctatgaaa aaccagatgc tcaatgcttc
      961 tcgcacgttc gttatttgct tggaggatac tttacgtatt gtgggatact actcattagc
     1021 tactggttcc gtcactcatg ccgagcttgg tcgcagtttg cggcataaca tgcccaatcc
     1081 tgtccctgtt gttctgttag ggcgtctggc tgttgatgtt tgtacccagg ggcatggttt
     1141 cggtaaatgg ctactaagtg atgccattca ccgggttgtc aatctggctg atcaggttgg
     1201 cattaaggct gttatggtac atgcaattga tgataacgcc agagcatttt atgagcgttt
     1261 tggttttgtt cagtcagttg tggcacctaa taccttgttt tataaagtct gagtctgagt
     1321 ctgaggtagc ctgagtttaa cggacactcc ttcctgaaat agaatggcat cagaaggagc
     1381 caataatgag cagaaaaaac caacgttact ctacgga
//
LOCUS       DADWTH010000282         1385 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.290, whole genome shotgun sequence.
ACCESSION   DADWTH010000282 DADWTH010000000
VERSION     DADWTH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.290"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..571)
                     /locus_tag="JNY02_004328"
                     /pseudo
     CDS             complement(1..571)
                     /locus_tag="JNY02_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(619..1113)
                     /locus_tag="JNY02_004329"
     CDS             complement(619..1113)
                     /locus_tag="JNY02_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9435562.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          422 a          279 c          261 g          423 t
ORIGIN      
        1 tcacactgat atgaattggg ttgggattct ctgctatcag atttcgccct tcccggcgcc
       61 atttcacttt cggcgcagca tcttcgggaa taaggcggat atttgctggt cgccagaaca
      121 gcttgatacg cgtttttact gctatttcca gcgagttcgt cgctttgttg ctcgctgatg
      181 gcggaatatt tttaatattc agccagaaga ctgactcgcg atcatcaggc aatgacacac
      241 ctttggtgtg aataatgcgt aatatttttt cttgcgaagg ttctaatcgt gatactggcg
      301 gaataacggt aaacggcgct ttgcttttat cctctttatt aaatggatct acccaagcct
      361 ggatgagata aggtattttt tcttctttgc taaatacaga aatagattta tcagcattat
      421 tacttaagta aaccacccgc gtactaccaa tgacaacgcc tgcataagtt aatgctggtt
      481 ggcctaaaaa aacgctgagt ataatcgcgg taaataactt acattaaact gttgttttaa
      541 agagcctgat taaacaactt tttctggaca taacgactcc ctggccaaag ccaattgaat
      601 aaaaaggccc ttgataaatt atttcttata ttcgacagta aattgtgaat cagcgttcgc
      661 cgtaccagcc gttacggtcg cattggttgc cacatagcgt gcaataaact tcatttcagc
      721 cttgccttca gcatcgatag cggtataatc agagtcgtta taaagtttaa cctgcgaatt
      781 atcggactgg ttaaaaatac gaataccaac gccggttgcc gcaatcgctt gtcctgtacc
      841 atcaaaacga acggctgctt gagtgtacgt tgccgggcaa ttttcaagtt taatactgaa
      901 cttctgtggc gacgccagac tacctacacc tgaaaatgca tttttactga ctttacccag
      961 tgggacaatt tgatcggctg tatcggaggt aacttcacaa gtagtgtcga caatttcacc
     1021 gacgaagtta acaacaccat cttctgccag tgcatgcccg gccagcatta gagatgccag
     1081 agtgaatgca ccgcctataa tattacgctt cataaaaact ccattgatat aatatatcct
     1141 ttaccatgaa tcatcactta tattaataag tgtatttttg attcaaagga atagtacacc
     1201 aaagagacgg ctttatctaa aaataatatt aattacgcaa aaataaaata tagatttatt
     1261 gtttaaatat tatttaaaca gtaaaaaatc aagtttgtta tcagacatta atgataattc
     1321 agaatatatt tatatagaaa gatagatgaa tttattgatg tcagttataa aaaagggcca
     1381 ccggt
//
LOCUS       DADWTH010000283         1360 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.291, whole genome shotgun sequence.
ACCESSION   DADWTH010000283 DADWTH010000000
VERSION     DADWTH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.291"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..921)
                     /locus_tag="JNY02_004330"
     CDS             complement(<1..921)
                     /locus_tag="JNY02_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBB9435563.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITE"
BASE COUNT          413 a          206 c          279 g          462 t
ORIGIN      
        1 ttcagtgatg actctgatat ggcgcgaatg taaattttgc ggcaacatct ggcgtaaaaa
       61 cagtctgttt tggtctgaca gggaaatacg aaactgaggc ccgttaaaat ttggaaccga
      121 agtttgctgt attaagaata gcagagtttg atctgataag gggttatttg caatctcaat
      181 agttatatga ggatctaaac gaaaaagatt tgtcgggatt gtagttattt ggttgttttc
      241 aaaataaaaa aatcttctca tgataatatt ttcttggatg tttggtaagt tgcttaatct
      301 attgctatat gcaaaaagaa gttgcagtct cctaggtaaa gggggcagtt cagtaagatg
      361 gttgtgcgca accttaagta tcttaagatt agaaggcaga gatggaagag tttgcagttc
      421 gttgttccta acctcaagtt tttttaaatt caagggtaat gaaggcagtg tggataacct
      481 attatactct gcgcttagtg atttgatggt gtctggtaat aacggcagat tctctaattg
      541 attttcggac acatccaaag ttattagata atgtggtaat gcagatatat gtgttaagaa
      601 attatctctt gcaataagtt ctttaatatt ttcaggtaaa tcagtaagga tggttaattt
      661 attacagctc acatcaagct ttttaatgca agcagggagt acaggtaacg aggaaagcgc
      721 caaggctcgc aggtccagtt tatgcccctt agaggtaaga catgcacgaa gtcttcttac
      781 cgcctcagta cgctgttctt ccggtaaccc ctgtttctcc caatcatccc attcggacaa
      841 ataatggttg ggggatgtaa ttgtgctgat atgtggaaat aaacaggatg aaatatttct
      901 gagcatatta atttctcgca tatggatatg gaattagatt gtgaataaat tgtatttaat
      961 gtatctctcc taatgaggag ggtgtttcag aatattcgcc ataagggaat gagtgttata
     1021 tcatgctttc taccctggta agtatatttg cttttttcgt atatttggtt catctttttt
     1081 tgatgtttta catattcaca atgcagagga tttataccag aagacattag tcttgagtaa
     1141 gatactacaa catctccaac tattttagcc agcctgacta cttgacttgg taatatttgt
     1201 tgctaataat catgtataaa ataagcttat atacatagta acttcagcaa aggatttagt
     1261 aatgatccaa atcatatctc aatgtactgt ttttttcttt aaaaaaacag tacattctat
     1321 tttataatga ctgtgtattc ccatggaagg agatcaccca
//
LOCUS       DADWTH010000284         1345 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.292, whole genome shotgun sequence.
ACCESSION   DADWTH010000284 DADWTH010000000
VERSION     DADWTH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.292"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            443..1192
                     /locus_tag="JNY02_004331"
     CDS             443..1192
                     /locus_tag="JNY02_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBB9435564.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          376 a          274 c          279 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aattt
//
LOCUS       DADWTH010000285         1296 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.293, whole genome shotgun sequence.
ACCESSION   DADWTH010000285 DADWTH010000000
VERSION     DADWTH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.293"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..815)
                     /locus_tag="JNY02_004332"
     CDS             complement(<1..815)
                     /locus_tag="JNY02_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBB9435565.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JNY02_004333"
                     /pseudo
     CDS             907..993
                     /locus_tag="JNY02_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          363 a          219 c          336 g          378 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaaatg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctt
//
LOCUS       DADWTH010000286         1277 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.294, whole genome shotgun sequence.
ACCESSION   DADWTH010000286 DADWTH010000000
VERSION     DADWTH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.294"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNY02_004334"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNY02_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HBB9435566.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          229 c          284 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DADWTH010000287         1227 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.295, whole genome shotgun sequence.
ACCESSION   DADWTH010000287 DADWTH010000000
VERSION     DADWTH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.295"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..884
                     /locus_tag="JNY02_004335"
     CDS             <1..884
                     /locus_tag="JNY02_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB9435567.1"
                     /translation="PTISLSAVIYFPPKFDETRQYPAIVVSHPGGGVKEQTAGTYVEK
                     LAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMG
                     ICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNART
                     SDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITY
                     DAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKA
                     YVAEAISVLAPFFEETL"
     gene            884..>1227
                     /locus_tag="JNY02_004336"
     CDS             884..>1227
                     /locus_tag="JNY02_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB9435568.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          325 a          279 c          317 g          306 t
ORIGIN      
        1 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
       61 agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa
      121 cctatgtcga aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
      181 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg
      241 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
      301 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
      361 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg
      421 aaaacaatgt gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa
      481 ccagcgatat tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg
      541 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt
      601 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
      661 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
      721 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
      781 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
      841 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
      901 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
      961 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
     1021 gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg ttctcgctta
     1081 tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag atccgacaca
     1141 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttgatgc
     1201 tgaacgcatt ggcgtgctgg gtgtgtg
//
LOCUS       DADWTH010000288         1210 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.296, whole genome shotgun sequence.
ACCESSION   DADWTH010000288 DADWTH010000000
VERSION     DADWTH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.296"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNY02_004337"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNY02_004337"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBB9435569.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..>1210
                     /locus_tag="JNY02_004338"
     CDS             943..>1210
                     /locus_tag="JNY02_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBB9435570.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSA"
BASE COUNT          280 a          327 c          297 g          306 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga
//
LOCUS       DADWTH010000289         1207 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.297, whole genome shotgun sequence.
ACCESSION   DADWTH010000289 DADWTH010000000
VERSION     DADWTH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.297"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            14..925
                     /locus_tag="JNY02_004339"
     CDS             14..925
                     /locus_tag="JNY02_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB9435571.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="JNY02_004340"
     CDS             941..1168
                     /locus_tag="JNY02_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435572.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          340 a          255 c          336 g          276 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gatataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggcaa
//
LOCUS       DADWTH010000290         1197 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.298, whole genome shotgun sequence.
ACCESSION   DADWTH010000290 DADWTH010000000
VERSION     DADWTH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.298"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..>1197
                     /locus_tag="JNY02_004341"
     rRNA            1..>1197
                     /locus_tag="JNY02_004341"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          334 a          261 c          372 g          230 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaacacta
      721 actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg ctgggggtga
      781 aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg tagcgcctcg
      841 tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg acttaccaac
      901 ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga ggcacacggc gggtgctaac
      961 gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag tcatggttaa
     1021 gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag cagccatcat
     1081 ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg taacggggct
     1141 aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg ggagcgt
//
LOCUS       DADWTH010000291         1185 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.299, whole genome shotgun sequence.
ACCESSION   DADWTH010000291 DADWTH010000000
VERSION     DADWTH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.299"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>1185
                     /gene="amn"
                     /locus_tag="JNY02_004342"
     CDS             <1..>1185
                     /gene="amn"
                     /locus_tag="JNY02_004342"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBB9435573.1"
                     /translation="AIGKYITIGELPDENARKQGLFVYPSLTVTWDGSTTNPPKTRAF
                     GRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPYVIDGSEL
                     TLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSLARLRHYT
                     GTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWITAETEAPEE
                     AISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDVWLMIGHC
                     GGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGE
                     EVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVPYGT
                     LLC"
BASE COUNT          287 a          312 c          276 g          310 t
ORIGIN      
        1 gccattggca aatatattac aattggcgaa ttacctgatg aaaacgcccg taaacaaggt
       61 ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaacaaa tccccccaaa
      121 acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat tactcgccct
      181 actctctttc gttcgtatct gaatgagcaa ctaacgttgc tttatcagga ttatggtgcg
      241 catatctctg tgcaaccctc gcaacatgaa atcccttatc cttatgtcat cgatggctct
      301 gaattgacac ttgatcgctc aatgagcgct gatttaactc gctacttccc gacaacagaa
      361 ctggcgcaaa tcggcgatga aactgcagac ggcatttatc atccaacgga attctccccg
      421 ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cacggttgcg ccattatacc
      481 ggtacgccag tagaacattt tcaaccgttc gtcttgttta ccaactacac acgttatgtg
      541 gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc ctacattgcc
      601 ctttcttgtg ctggcgggaa ctggatcact gccgaaaccg aagcgccaga agaagccatt
      661 tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac cgctgatggt
      721 cagggtatta ctctggtgaa tattggcgtg ggaccgtcca atgctaaaac catctgcgat
      781 catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg cggattacgt
      841 gaaagccagg ccattggaga ttatgtcctt gcacacgctt atttacgcga tgaccacgtt
      901 cttgatgcgg ttctgccgcc cgatatacct attccgagca ttgctgaagt gcaacgtgcg
      961 ctttatgacg caaccaagtt agtcagcggc aggcctggtg aggaagtcaa acagcggctg
     1021 cgtacaggta ctgtggtaac cacagatgac aggaactggg agttacgtta ctcagcttct
     1081 gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc aaccattgcc
     1141 gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgt
//
LOCUS       DADWTH010000292         1166 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.300, whole genome shotgun sequence.
ACCESSION   DADWTH010000292 DADWTH010000000
VERSION     DADWTH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.300"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..161
                     /locus_tag="JNY02_004343"
                     /pseudo
     CDS             1..161
                     /locus_tag="JNY02_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            178..696
                     /locus_tag="JNY02_004344"
                     /pseudo
     CDS             178..696
                     /locus_tag="JNY02_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          354 a          267 c          250 g          295 t
ORIGIN      
        1 gtgacagact ggcacgtctt cgtaaggagc tgaggctgca ctgtaagcag aaacgcaagt
       61 tcagggcgac cacaaactct gaccacaatc tgccggttac gccgaatctg ctgaatcaga
      121 actttacgcc cacagcacct aatcaggtct gggtcgcaga ttcagttgtc caggccttcc
      181 gaaatcaacc gactgaaggc gctggtcgcg aaactgcagc gtatgcagtt cggtaaaagc
      241 tcagaaaaac ttcgcgcaaa aaccgaacgg cggatacagg aagcacagga gcgaatcagc
      301 gcacttcagg aggaaatggc ggaaacactg ggtgagcaat atgacccggt actgccatct
      361 tccctgcgcc agtcttcagc ccgtaaaccg ttaccagcct cacttccccg tgccccccgg
      421 gtcatccggc cggaagagga atgctgtccg gcctgtggtg gtgaactcag tccactggga
      481 tgtgatgtgt cagagcagct ggagcttatc agcagcgcct ttaaggttat cgaaaaacaa
      541 cgccctaaac tggcctgtcg ccggtgcgac catatcgtgc aggcaccggt gccttcaaaa
      601 cccattgcac gcagttatgc cggagcgggg cttctggccc atgttgttac cggaaagtat
      661 gcagactatc tgccattata ccgccaaagt gatctactat tccacacagc tatttagctc
      721 tctttttgtt gagctcccat gattctttca tttattgtta ctaattattt tatgttatca
      781 tgatgttaga acacgctctg ctctgtgtct ctattttatc tatcatattc tgaggattaa
      841 ctacataaca aggcgcaaga tatatatcca aaaacaaata ataccgttgc aataattcct
      901 ttcagtaaag gaggtgtgat taatttcagg ggatttcaga gtaaaaattg tacaacatat
      961 taaaatatgt tcatttcgtt ataatacaca caagaaaata ttaagttaga taatatattc
     1021 taaataacca tagataaaaa cgcacataat ggggaatatt ttatgaaaat acctgaagca
     1081 gtaaatcata ttaatgttca gaataatatt gatcttgttg atggaaaaac gaatccgaat
     1141 aaagcaacaa aagcattaca aaaaat
//
LOCUS       DADWTH010000293         1161 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.301, whole genome shotgun sequence.
ACCESSION   DADWTH010000293 DADWTH010000000
VERSION     DADWTH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.301"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(133..810)
                     /locus_tag="JNY02_004345"
     CDS             complement(133..810)
                     /locus_tag="JNY02_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435574.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
     gene            complement(946..>1161)
                     /locus_tag="JNY02_004346"
     CDS             complement(946..>1161)
                     /locus_tag="JNY02_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HBB9435575.1"
                     /translation="DNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSR
                     SETVLQKSLEITEEADLALRNAMARIK"
BASE COUNT          289 a          283 c          257 g          332 t
ORIGIN      
        1 cgcctctgta gtattaacgt atttgatgtt gtttggcagt tttacttgtt tcgccgtagc
       61 gaagacagag aacagaatag ttacagcaac gagtaaagtt ttgataaaag ttttcatgac
      121 agactcctta aattagttaa ccgcagctac gtagtttttc accagatacg cgaattcatg
      181 cacgccaatt tcgccgaacc aaaccaggta ggccgggtca tctaaaccaa agtagcggta
      241 gccaacgact tcatgggttt ttgggttata gagtgttacg ataaagtccc agccgcaggt
      301 attggctttg caaccgtcgc cctggagcca gactgtaccg ttaatggtta ctggcgttac
      361 gtttccgcca gggccatcca tatacccgcc tttaccgaac atgccgctca gtccgaccgg
      421 ttttaatgct ttgtcaagcg cagctttgta ggcagcgtca ccttcgtaca actcgtacgt
      481 tgttggacac gctccaggtt gttcagcccg ggcgtttggg tcgcagctga gcgcttgttt
      541 cacgctcgct gcttccacgt taaagccagt aaacagaacc gccagaaata acatcagggt
      601 atgtaaacgt ttcattgtaa atattcttcc gttgtaaatg gcgctggaaa atttctcatc
      661 agtgaaacct tccggaaatt tcccaccctt gtatgatgta tatcgccagt attttatttc
      721 atcccggata taatttttat atttcccggc acgtgcaata acgctgacca gagatataag
      781 gctcattaat aactttacag gtaattgcat aattaaattt ccagtgagtg aattgacagg
      841 tgaggggtaa aactttctct caggtaatat atatctgaaa atattttcaa taacaccagc
      901 taggaaaaac accaccacag gcggtggcgt ttctttggag cgtctttatt taatccgggc
      961 catcgcgttt ctcagtgcca aatctgcctc ttccgttatc tccagacttt tctgcaacac
     1021 cgtttctgac cgggacagta cctgacgtgc tgccagcggt ttccggatat gctccatcgt
     1081 cagcatcgtg tcttcatagc cctgctgacg ccaggcgtcg gtatgtgcca acgtgaaact
     1141 gagcagaccg ccgctgttgt c
//
LOCUS       DADWTH010000294         1158 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.302, whole genome shotgun sequence.
ACCESSION   DADWTH010000294 DADWTH010000000
VERSION     DADWTH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.302"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..387
                     /locus_tag="JNY02_004347"
     CDS             <1..387
                     /locus_tag="JNY02_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435576.1"
                     /translation="LVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(388..1032)
                     /locus_tag="JNY02_004348"
     CDS             complement(388..1032)
                     /locus_tag="JNY02_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB9435577.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          263 c          278 g          322 t
ORIGIN      
        1 ctggtgctgg tggcaattct gattatgctg ccgactctcg gcatcggtga tatgaacaat
       61 ctctataaat ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta
      121 tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt
      181 attcgtaata agcctctggc aatgaccgtc gggatttggt gttttgcctt taccgcctgt
      241 gcctgtttga cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag
      301 ttggcgctga atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg
      361 ctggcgcgta aagcgaatag caaataatta ttgtggtcgg tgacgctgac tctggcagat
      421 ctgacccgtt tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt
      481 tgggtaatag acttggaagc ctggacgctc tgtgatccag tcttccagta cacattctaa
      541 acgaccagta tcaaggtact ccttgatctg ttcatagagc agatacccga taccagcacc
      601 catcaaaacg gcctccagtt cagcatcaac atcatccaga atgatatttc ctgcgacagc
      661 aatttccagc tcctttgcaa attgccagtg gaaaggtttg ccgctgggat aacgaaaaac
      721 aacacattga tgattaagca gatcatgagg atggcggggt ttgccatatc ttgcaaagta
      781 ctctggcgtt gctgctacac ataatttcac tggtgggccg atcgccacgc tgatcatatc
      841 tttttcgacg atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat
      901 aagagaatcg tcagttgtga gctctacttt aatatcagga tattcacgtg tgaaaccaac
      961 cagtaaaggc attaaaaata tacgtgcagc tacccgggcg gcattgattt tgagcgttcc
     1021 tgtcggtgtc agacgaaaat cattcatctc atctaacatg atctgaattt cgtcgaaagc
     1081 tgggcgcaat cgttcataga gattagaacc cgcttccgtt aacgatacgc ttcgggttgt
     1141 ccgattgaat aagcgaat
//
LOCUS       DADWTH010000295         1149 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.303, whole genome shotgun sequence.
ACCESSION   DADWTH010000295 DADWTH010000000
VERSION     DADWTH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.303"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..231)
                     /locus_tag="JNY02_004349"
     CDS             complement(<1..231)
                     /locus_tag="JNY02_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB9435578.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQ"
     gene            complement(498..1004)
                     /locus_tag="JNY02_004350"
     CDS             complement(498..1004)
                     /locus_tag="JNY02_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435579.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQSDLSQGDQLTEMLSFLTSEEREAL
                     TNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEASILS
                     ESVNAKAG"
BASE COUNT          296 a          265 c          241 g          347 t
ORIGIN      
        1 ttggctggct gcaaccgcta atgggctgga accgaggttg ccgatttgca cgtcgccaga
       61 agccagcgcc cgcacgatgc tggctccgct gtcaaactta cgccagtcca cgctcgcccc
      121 gctctcttta gcaaaggtgt tgtcggcctg agccactttc gccggttcgg ctgaggtttg
      181 atacgccacg gtgacgttca ccgcctgtgc ctgaaaagcg atgaatgcca gtgcggcaag
      241 aagtgtgtta tatgactagg taatttattc aagataaccg ggaatgggta aaaaaagacg
      301 ggatttgatg ggattcaata agttatgcgc cgattgcatt caagataaaa cgagttaaaa
      361 atgctcaatg cttcgatatc gataaaacat tcaagatagg cgcgccgtgt attttaattc
      421 ttttaagact cttcaaaaat gtcatcgtag cctttaaaaa cgaattttaa aggcacccta
      481 aaggcaggtc aatgaagtta acccgccttc gcgtttacac tctctgagag aatactagct
      541 tcaatctctt ccaaaatctc tctgacttgc tcgtcgggca tatcaacgag catcaacgct
      601 gccaggcgtt tacggccctc tagttgcagt agctcttgag tttccttatc taacaagcgt
      661 agcacagtct caacaccttt ccttgcgagc atgtttgtaa gggcttctcg ttcctcactg
      721 gtaagaaacg acaacatttc tgttagctga tccccttggc ttaaatcact ttgtgttttt
      781 ggtggattcc cgttaccggt ttttagccat tcaagactaa caccttcact ttctgcaatg
      841 cgtaccaata tgctttctcg aggttctggt gtatttccct ttttaaaata gctttgtaac
      901 gtattgatat ttatgccaca ggctctggcg gcagcacttc ttgatggata ccgcctaata
      961 agataagcca tgcgctccac gataggttct tttgcctgct ccataaaagt acctctaggt
     1021 atttttctgt ataaaggttc ttttccatct atctaattga atggaaaggt aattttaaaa
     1081 atccaaataa aatacaaaaa aataccttgt attggtgctt tttgtgatct gcgattttat
     1141 ttactcgga
//
LOCUS       DADWTH010000296         1141 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.304, whole genome shotgun sequence.
ACCESSION   DADWTH010000296 DADWTH010000000
VERSION     DADWTH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.304"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..645
                     /locus_tag="JNY02_004351"
     CDS             <1..645
                     /locus_tag="JNY02_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
                     /protein_id="HBB9435580.1"
                     /translation="EAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQRGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            642..>1141
                     /locus_tag="JNY02_004352"
     CDS             642..>1141
                     /locus_tag="JNY02_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBB9435581.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGR"
BASE COUNT          299 a          275 c          322 g          245 t
ORIGIN      
        1 gaggctgaag cacagttccg gcctgagctg gagaaaatcg tcagcgggat tgaaacaggt
       61 tttcgtggca cggccctgta cgccacagaa aacattgccg gtcgcatcaa tacccgcctg
      121 gaggatgagg gctttaccgt aaaaatcagt tttccggcag tcagtcagtt acaaacccgg
      181 ctcgcggtaa aaacaaatct gagtgcgctt atggaggaaa gaacggagac cgtcacccgt
      241 cgccgtcggc agcgtggttt atggggaaag atttgtggag cgtttggcac cagtgactgg
      301 ggctgggaaa cctataaaga ggatgtgagc cgcagtgtta tcaatatcaa caccgtcagg
      361 aaggaagtta tgtcactgac ccgggcatat ttcggggagc tgcaggcatc cattgagcag
      421 gatattaacc agcccgtccg ccaggaaatc gatgcctttt tctgcgcatt cagggagaaa
      481 gttgaacaac tgcgtaacac gctgattcag agctctgaag atcataaacg cgatcagcag
      541 gcacaggaac ggcttaccag gcgacttcag gcattaaatg aaagggttcc tgagctcatt
      601 actgacagta aggcgctgag ggaagagctg gagacaatgc tgtgacttca ccatttattc
      661 aacaaattgc tgataaccgg gtatgtcagg tactcacctg tcttcctgaa aaatttgtgg
      721 ttgatttcgc aaacggaatc gatgttgcac aggagcatat ccgcacggcc ggagagcgca
      781 cgttcttccg gcgtttaaaa gagggactga ccggcgaagg cgctgcacgg cagaatgcca
      841 tcaatgcttc ggttgcacag ggcgttgagg cgtccctgcg ctggctgacg gagctgacaa
      901 cgtcacttgc caccacaaac tacgcgatta cccgggtaaa cgacagggtc agttcactgg
      961 tcagtgatac tgccaggttg gcgcattatt ccgcagatac acgggagcag ttactcatcc
     1021 tggccgatca ggttcaccat aaactgaatc atcttgaaga aaaactccac cgtgttgacc
     1081 aggttcagcg ggcgcagtta catcttgagc agatattctc atggtggagc gccgggcgat
     1141 a
//
LOCUS       DADWTH010000297         1141 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.305, whole genome shotgun sequence.
ACCESSION   DADWTH010000297 DADWTH010000000
VERSION     DADWTH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.305"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..645
                     /locus_tag="JNY02_004353"
     CDS             <1..645
                     /locus_tag="JNY02_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBB9435582.1"
                     /translation="EAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            642..>1141
                     /locus_tag="JNY02_004354"
     CDS             642..>1141
                     /locus_tag="JNY02_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBB9435583.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGR"
BASE COUNT          298 a          268 c          327 g          248 t
ORIGIN      
        1 gaggctgaag cacagttccg tcctgaactg gaaaaaatcg tcaggggtat tgaaacaggt
       61 tttcgtggta cggccctgta cgccacagaa aatattgccg gacgcatcaa tgcccgtctg
      121 gaggatgaag gtttcactgt aaaaatcagt tttccggcag tcagccagtt acagacccgg
      181 ctcgcagtaa aaacaaatct gagcgcgctt atggaggaaa gaacggagac agtaacccgt
      241 cgctgtcgac aggatggtgt ctggggtacg ctttgtcgat gggcaaacac cagtgactgg
      301 ggctggaaag agtacagtgt ggatgtgagc cgcagcgtga tcaatatcaa caaggtcaga
      361 gaggaagtga tgtcactgac ccgggcatat ttcggggagc tgcaggcatc cattgagcag
      421 gatattaacc agcccgtccg ccaggaaatc gatgcctttt tctgtacatt cagggagaaa
      481 gttgaacagc tgcgtaacac gctgattcag agctctgaag atcataaacg cgatcagcag
      541 gcgcaggaac ggctgaccgg gcgtcttcag gcgttaaatg aaagggttcc tgagctcatt
      601 actgacagta aggcgttaag ggaagaactg gagacaatgc tgtgacttca ccatttattc
      661 aacaaattgc tgataaccgg gtatgtcagg tactcacctg tcttcctgaa aaatttgtgg
      721 ttgatttcgc aaacggtatt gatgttgcac aggagcatat ccgtacggcc ggagagcgca
      781 cgttcttccg gcggttaaaa gaggggctga gcggcgaagg tactgcacgg cagaatgcca
      841 tcaatgcttc gcttgcacag ggcgttgagg cgtccctgcg ctggctgacg gagctgacaa
      901 cgtcacttgc caccacaaac tacgcgatta cccgggtaaa cgacagggtc agttcactgg
      961 tcagtgatac tgccaggctg gcgcattatt ccgcagatac gcgggagcag ttactcaccc
     1021 tggccgatca ggttcaccag aaactgaatc atcttgaaga aaaactccac cgtgttgacc
     1081 tggttcagcg ggcgcagtta catcttgagc agatattctc ctggtggagc gccgggcgat
     1141 a
//
LOCUS       DADWTH010000298         1126 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.306, whole genome shotgun sequence.
ACCESSION   DADWTH010000298 DADWTH010000000
VERSION     DADWTH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.306"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            252..1007
                     /gene="mlaA"
                     /locus_tag="JNY02_004355"
     CDS             252..1007
                     /gene="mlaA"
                     /locus_tag="JNY02_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBB9435584.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          276 a          260 c          293 g          297 t
ORIGIN      
        1 gcttttttgc tcgcaactgg cgggagttac tctgaaaatg tagaaaaggt tgcgtttgcc
       61 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
      121 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
      181 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
      241 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
      301 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc
      361 gcaccatgta caacttcaac ttcaacgtat tagacccgta tattgttcga ccggtcgctg
      421 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaatgg tttgagcaac tttactggca
      481 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
      541 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
      601 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
      661 ttggtcatta tggcgtgggt tatgggcctt acgttcagtt accgttctac ggtagcttca
      721 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
      781 cctggccgat gtctgtgggt aaatggacgc ttgaagggat cgaaacccgc gctcagctgc
      841 tggattccga tggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
      901 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
      961 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa
     1021 aaggtgagtc gcaaaactca ccttttttgt tgttatccct caattaccac aagtaaaagg
     1081 agataaaccg acaatgaccc ggacgtaatg atgtaaaaat actgat
//
LOCUS       DADWTH010000299         1121 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.307, whole genome shotgun sequence.
ACCESSION   DADWTH010000299 DADWTH010000000
VERSION     DADWTH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.307"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>1121
                     /gene="pdeF"
                     /locus_tag="JNY02_004356"
     CDS             <1..>1121
                     /gene="pdeF"
                     /locus_tag="JNY02_004356"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBB9435585.1"
                     /translation="TYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLP
                     LALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFT
                     PRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGT
                     LINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLAL
                     GALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSY
                     QNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLP
                     NVRALNRALRDALWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          245 a          254 c          251 g          371 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa
       61 tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc
      121 tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat
      181 tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa
      241 cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc ggttacaggg
      301 tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta
      361 tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat
      421 ttgtaggatt actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga
      481 ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact
      541 tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac
      601 agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg
      661 ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt
      721 ataccttgtc attattgctg cccctgatga tgtggggagc aatgcgctat ggttataagc
      781 tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca
      841 ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat
      901 tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac
      961 gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga
     1021 atcgcgcgtt gcgtgatgcc ctctggtctg cgctttgtta tttacgcatc cctggcatgg
     1081 aaatgctggt taagaactat ggcatcatgc tgcggattca a
//
LOCUS       DADWTH010000300         1114 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.308, whole genome shotgun sequence.
ACCESSION   DADWTH010000300 DADWTH010000000
VERSION     DADWTH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.308"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(3..76)
                     /locus_tag="JNY02_004357"
     tRNA            complement(3..76)
                     /locus_tag="JNY02_004357"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Arg,seq:tct)
     gene            complement(78..153)
                     /locus_tag="JNY02_004358"
     tRNA            complement(78..153)
                     /locus_tag="JNY02_004358"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Ile,seq:cat)
     gene            complement(330..1019)
                     /locus_tag="JNY02_004359"
     CDS             complement(330..1019)
                     /locus_tag="JNY02_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBB9435586.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQGNAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRLEQKA
                     AFSKQSIAKVD"
BASE COUNT          272 a          308 c          227 g          307 t
ORIGIN      
        1 ggcccaccac cgaggacttg aacctcggac cgtcaactta gaaggttgat gctctatccg
       61 gatgagctag tggtggttgg tggcccttgc tggacttgaa ccagcgacct ggcgattatg
      121 agtcgctcgc tctcaccact gagctaaagg gccggaggca gaataataac catatgtcat
      181 cacatctgca aactcatctg accaccagca cgtttaacgt cctgtgccat ttttcaggca
      241 taaaaaaacc gctcggcggc gggttttgct tactttacca tcgcgtacaa aatcggcaaa
      301 gtatcagatt tgcatgaaat atatgccttt taatctactt ttgcaatact ttgctttgaa
      361 aatgccgctt tttgttctaa acgtgttctc atcacaaaca ataaagcctc actatccagt
      421 cggtgaaaaa tgtgtttcat tacaacccag tgaccagtaa atgttttgga ccagtttttg
      481 gttgttactc ccgccagtaa tgccagctcc tgatattcat aaccttcccc accaaaaagt
      541 tctgctttta ccgcctgcgc cgccagccag attaattttt tcaggcgttc ctgtgttttc
      601 tctgcaattt tcctagtgcc agaatgagcc ttaaactctt cccacgccca gtgggttatg
      661 gtgatctgat actcccagca aatacttccg ctgtaacacc acaacaacca ggctttatgg
      721 tgctcttcaa gagacagaac agcccgccgc cacgatgatg tcgaaaacgc aacctgactg
      781 accagaggaa ttgacgcccc cttcgccagt gattgttttc ccgggattgg tggattatcc
      841 cgcgttatca tttttccggt cacctcatcg cggtaccgga tttttttacg cctgtaacgc
      901 cctgtatcga acatggcatt cccctgccag gcttcaagct gaccttttgt cgccccactc
      961 aaatcagcgg tggcgataat gagctgctca cgaacaaact gtaaatactg gttattcatg
     1021 cacactccag ctctgtgatt tttatcccca accgaccacc aggaacaggc agtccgcgca
     1081 caatattggt ttcatcaaac tgctcgtcgt cgat
//
LOCUS       DADWTH010000301         1110 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.309, whole genome shotgun sequence.
ACCESSION   DADWTH010000301 DADWTH010000000
VERSION     DADWTH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.309"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..1019
                     /locus_tag="JNY02_004360"
                     /pseudo
     CDS             1..1019
                     /locus_tag="JNY02_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          316 a          270 c          255 g          269 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctagacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcatt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DADWTH010000302         1107 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.310, whole genome shotgun sequence.
ACCESSION   DADWTH010000302 DADWTH010000000
VERSION     DADWTH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.310"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..387)
                     /locus_tag="JNY02_004361"
     CDS             complement(<1..387)
                     /locus_tag="JNY02_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HBB9435587.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="JNY02_004362"
     CDS             816..1097
                     /locus_tag="JNY02_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBB9435588.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          307 a          249 c          252 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctgc ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DADWTH010000303         1090 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.311, whole genome shotgun sequence.
ACCESSION   DADWTH010000303 DADWTH010000000
VERSION     DADWTH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.311"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>1090
                     /locus_tag="JNY02_004363"
     CDS             join(<1..250,250..>1090)
                     /locus_tag="JNY02_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HBB9435589.1"
                     /translation="VSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYN
                     WRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKYVFIE
                     KHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYG
                     APRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDF
                     YASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMAL
                     WRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKV
                     ECIHGEHFISREIMRATVFNYIECDYNRWR"
BASE COUNT          271 a          283 c          308 g          228 t
ORIGIN      
        1 gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag tgaagccctg
       61 aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct gtatgaatca
      121 cagctctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga acgtgaactg
      181 gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga tgaagagctg
      241 gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag tatgtcttta
      301 ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc cgggtggccc
      361 gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt cagcagttcc
      421 gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag cgttacggtg
      481 ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta aaaaccgtgg
      541 cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaagttc agcccggtca
      601 gctaccgcgc acacggcctg cctgtgtcag aaaatctgtt ggagcaggat ttttacgcca
      661 gtggcccgaa ccagaagtgg ccaggagaca tcacgtactt acgtacagat gaaggctggc
      721 tgtatctggc agtggtcatt gacctgtggt cacgtgccgt tattggctgg tcaatgtcgc
      781 cacgcatgac ggcgcaactg gcctgcgatg ccctgcagat ggcgctgtgg cggcgtaaga
      841 ggccccggaa cgttatcgtt cacacggacc gtggaggcca gtactgttca gcagattatc
      901 aggcgcaact gaaacggcat aatctgcgtg gaagtatgag cgcaaaaggt tgctgctacg
      961 ataatgcctg cgtggaaagc ttctttcatt cgctgaaagt ggaatgtatc catggagaac
     1021 actttatcag ccgggaaata atgcgggcaa cggtgtttaa ttatatcgaa tgtgattaca
     1081 atcggtggcg
//
LOCUS       DADWTH010000304         1088 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.312, whole genome shotgun sequence.
ACCESSION   DADWTH010000304 DADWTH010000000
VERSION     DADWTH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.312"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(184..1053)
                     /locus_tag="JNY02_004364"
     CDS             complement(184..1053)
                     /locus_tag="JNY02_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBB9435590.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          273 a          317 c          231 g          267 t
ORIGIN      
        1 acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat tcagtgtgcc
       61 acttcaatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc ataattacct
      121 gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc cgtttacttc
      181 tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg ccgttactgc
      241 aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct catctgtaga
      301 taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc ccgcccccgt
      361 gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc gataacagac
      421 caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact cttttgccag
      481 ggcgcatata tcctgatcgt tctgtgtttt tccactgata agtcgccatt cttcttccga
      541 gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga gcaaatgctc
      601 gtcttgccat agatcttcac gaatattagg atcgaagctg acaaaacctc cggcatgccg
      661 gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca acgcaattga
      721 acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct ctaaaaaaag
      781 atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt tcagatcgac
      841 aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat cgactccctc
      901 agttagcagc gttctttgca ttaacgcccc aaaaggatca tcaccgaccc gacctataaa
      961 cccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg cgccgccagg
     1021 acaaggcagt aggcgcccgt ctgattctgg caagagatct acgaccgcat cccctaaaac
     1081 ccatactt
//
LOCUS       DADWTH010000305         1071 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.313, whole genome shotgun sequence.
ACCESSION   DADWTH010000305 DADWTH010000000
VERSION     DADWTH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.313"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..767
                     /locus_tag="JNY02_004365"
     CDS             <1..767
                     /locus_tag="JNY02_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB9435591.1"
                     /translation="FATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            788..1039
                     /locus_tag="JNY02_004366"
                     /pseudo
     CDS             788..1039
                     /locus_tag="JNY02_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
BASE COUNT          346 a          239 c          244 g          242 t
ORIGIN      
        1 aatttgcaac agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa
       61 aaaagtccaa gatagaacca gatcccctca cccgtgacga atttattcgt tttattgagg
      121 cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc
      181 acggggagct ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca
      241 tccgccggaa ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg
      301 gaagaacgat tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga
      361 ccatgcttgg aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg
      421 tgcataaatg cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac
      481 cgcactataa ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc
      541 tgagacacag aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag
      601 gagctaatcc tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca
      661 atgtttacgg agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa
      721 gactatctga aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc
      781 aaaatcaatt ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg
      841 attgctgaaa ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga
      901 ccggatccgc gagatcaaat gctggatcga caacggcgtg caggtcagca aagttaaaat
      961 actgctcagt aatgaaaatg ttgatgtgca gaacgtctgg cgcgatcagc aagaaacatt
     1021 actgacttac ctgcaaagcg tgaaccgccc cgggaatcct ggagactaaa c
//
LOCUS       DADWTH010000306         1025 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.314, whole genome shotgun sequence.
ACCESSION   DADWTH010000306 DADWTH010000000
VERSION     DADWTH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.314"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>1025)
                     /locus_tag="JNY02_004367"
     CDS             complement(<1..>1025)
                     /locus_tag="JNY02_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB9435592.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPY
                     VNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKL
                     GEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGT
                     PVNGYFL"
BASE COUNT          303 a          268 c          219 g          235 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gagaatacca
      121 gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa gtattaccca
      181 tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttcgtc attaagtcat
      241 ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga atggcacaaa
      301 ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct gggttgcgcg
      361 tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc agcattgaaa
      421 tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta atgtttgtgg
      481 tcgcaatatg cacttcagta atcgcaggcg cggttaccgc catcgccaca tacaggagcg
      541 acataacaaa catcgcaatc cctgccacag aaccgacgat ttttagcgat ttcataccgc
      601 gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt aacgctacga
      661 ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc gcaatcagaa
      721 ttgcctgggg tttttgtgcc agatagggaa tatgcaccac ccagtaggtc cacgcggcga
      781 gataagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg cccttcccat
      841 ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa tagagtgcaa
      901 agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta ttaacaacgt
      961 tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat cgcaactgtt
     1021 tttcc
//
LOCUS       DADWTH010000307         1016 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.315, whole genome shotgun sequence.
ACCESSION   DADWTH010000307 DADWTH010000000
VERSION     DADWTH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.315"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..273
                     /locus_tag="JNY02_004368"
     CDS             <1..273
                     /locus_tag="JNY02_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBB9435593.1"
                     /translation="IRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSH
                     ADTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            275..>1016
                     /locus_tag="JNY02_004369"
     CDS             275..>1016
                     /locus_tag="JNY02_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435594.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTV"
BASE COUNT          323 a          186 c          200 g          307 t
ORIGIN      
        1 atccgtagaa atgatggttc gatccgcttt aacaataaga tgccttatca cgttacgctg
       61 ggttatatcg gaacaaacgg aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc
      121 ccttatagcc atgcagatac gcagtttaaa aatgttccgt cagcttttca ggttggttat
      181 atcaatgatt ttggtggatt aagtttttat gaaataaact gcccgacggt taataactct
      241 tgtaatgttt ccgtagccaa acgagataaa taatatgcgc tacttattaa ttgtaattat
      301 atttattata ggttttagtg cattacctgt atgggcaatg gactgctatg ctgaacatga
      361 agagggaaat accgtagtca ttggttacgt accaagaatt tctatcccca gtaatggtaa
      421 aaaaggtgat aagatctggc aaagcagtga gtattttatg aatgtcttct gtaataatgc
      481 attacctgcc ccatcacctg gagaagaata cccatcagcc tggactaata taatgatgtt
      541 tttaccaggc ggtcaggact tttataatca gaactcctat atttttggcg taacatacaa
      601 tggagttgat tatgattcga ctgcccctaa tgctatcgca gcaccagaat gcattgatat
      661 aaaaggagcg ggtaccttta ataatcacta taaaaacccc gctgtctgta gtggaggtcc
      721 tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa ctctatatta aattagcaaa
      781 aaatgccaat agagttaata aaaacctggt gttacctgat gaatatatcg ctccagagtt
      841 taaaggtatg agtggcgcag gagctataga agtagacaaa aatttgacct tcaggattcg
      901 tggattaaat aatattcatg tccttgactg ctttgttaat gtcgcactgg aaccagctga
      961 tggcgttgtt gactttggta aaataaattc ccgaacaatt aaaaatacca ccgtta
//
LOCUS       DADWTH010000308          990 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.316, whole genome shotgun sequence.
ACCESSION   DADWTH010000308 DADWTH010000000
VERSION     DADWTH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.316"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            82..>990
                     /locus_tag="JNY02_004370"
     CDS             82..>990
                     /locus_tag="JNY02_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HBB9435595.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DADWTH010000309          983 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.317, whole genome shotgun sequence.
ACCESSION   DADWTH010000309 DADWTH010000000
VERSION     DADWTH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.317"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNY02_004371"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNY02_004371"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBB9435596.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGTENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDHLVSPEGLAPEWD"
BASE COUNT          259 a          218 c          221 g          285 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggt
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag tgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADWTH010000310          977 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.318, whole genome shotgun sequence.
ACCESSION   DADWTH010000310 DADWTH010000000
VERSION     DADWTH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.318"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            87..977
                     /locus_tag="JNY02_004372"
                     /pseudo
     CDS             87..977
                     /locus_tag="JNY02_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
BASE COUNT          258 a          229 c          242 g          248 t
ORIGIN      
        1 gttgtttact gtgcgctgtt gtccatgtga tattgtgtac cagatgtgtg ccaaactatt
       61 atttctgagc gtaccaaata ttatttatgg ctataagcga cacaaaactg cgtactattt
      121 atggtaaacc atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa
      181 tttcaccgaa gggggtcatc cagttccagt accgctatcg ctggcatggc aagcctaatc
      241 gacttgggct tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg
      301 acttgcgaaa gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg
      361 agaactccat gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa
      421 ctctcagaga aaaggcctag gcactttatc gatcaacggt tatgaagcgc atgcatgacg
      481 ccttccctaa tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg
      541 aagaagaaaa agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa
      601 ttagttggtg catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa
      661 gggacttcgg ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta
      721 agatttggct tgctattgaa agaagccgtg cgtctacgtc aaataagctc cttcatcaga
      781 tgcttatgct gtggggggcg aggctctcag agcttaggct ggcaacaaag acagaatttg
      841 acctgctgga caacgtatgg accgtaccga aagagcatag caagatgggt aatgttatcc
      901 gccgtccaat cttcgaacaa attaagcctt ttctcgaaaa ggccatgaca atgtacaatg
      961 atgttctttt ccctgga
//
LOCUS       DADWTH010000311          967 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.319, whole genome shotgun sequence.
ACCESSION   DADWTH010000311 DADWTH010000000
VERSION     DADWTH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.319"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>967
                     /locus_tag="JNY02_004373"
     CDS             <1..>967
                     /locus_tag="JNY02_004373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBB9435597.1"
                     /translation="YIARLRNRVENRLWHSLAACIDDSQTQQLLDLLSVPAGSRYSLL
                     DQLRAGPTKVNATSLVQAIGRLQTIRSLGVTLPAITPVSDIRIAAMARYASTAKITAL
                     QRLPEKRKLATLVAFSCCMEATAQDDALELLEALLRDLFNEAVQADKRNRQRTLKDLD
                     RAAEILAKACRMLLDDKLSDTDVRDSIFNIIPEDVLTHAVNNVTSIIRPDNNVYFNEL
                     DSKFKTVRRFLPDLLSRIHFEGNASAKTLIEALCWIEVNLKKKKTDNDAPREIINKPW
                     QQHVIRKDGSIDFHAYTFCALKELQLALKKRDIYVNPSWRYADPRA"
BASE COUNT          289 a          215 c          204 g          259 t
ORIGIN      
        1 tatatagccc gattacgcaa cagagttgaa aaccgtctct ggcattcact ggcagcttgc
       61 attgatgact ctcagactca acagcttctg gatcttcttt cggttcctgc cggaagccgc
      121 tattcgttac ttgatcaatt acgcgccgga cctactaaag tgaatgccac atcactggta
      181 caggctatag gtcgccttca gacgatacgt agcctaggag tgacattacc tgcaattacg
      241 ccagtttcgg atatccgtat tgcggccatg gcacgttatg cctcaacggc taagataaca
      301 gcgcttcagc gcttaccaga aaaaagaaaa ctggctacgc ttgttgcatt ctcttgctgc
      361 atggaggcga cagctcagga tgatgctctt gagttacttg aagcgctact ccgggatctg
      421 tttaatgaag cggttcaagc agataaacgt aaccgccaac gaacacttaa agatctggat
      481 cgtgcagcag aaatactggc aaaagcatgt cggatgcttc ttgacgataa attatctgat
      541 actgatgttc gtgatagtat cttcaatatc attcccgaag atgtactgac tcatgcagta
      601 aataatgtga catcaattat tcgaccagat aataacgttt acttcaatga acttgattct
      661 aaattcaaaa cggtgcggcg tttcttacct gacttacttt ccagaataca ttttgagggc
      721 aatgcctcag ccaaaacatt aattgaagcg ctttgctgga ttgaagttaa tttaaaaaag
      781 aaaaaaacag ataacgatgc accacgcgaa ataatcaata aaccgtggca acagcatgtg
      841 ataagaaaag atggtagtat tgatttccac gcctatacat tttgtgcact taaggaactt
      901 cagcttgcac tgaaaaaacg ggatatttat gtcaatccca gctggcgtta tgcagatccc
      961 cgggcag
//
LOCUS       DADWTH010000312          937 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.320, whole genome shotgun sequence.
ACCESSION   DADWTH010000312 DADWTH010000000
VERSION     DADWTH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.320"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>937
                     /locus_tag="JNY02_004374"
     rRNA            <1..>937
                     /locus_tag="JNY02_004374"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          222 a          209 c          299 g          207 t
ORIGIN      
        1 ggaaggttaa ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg
       61 cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg
      121 cacgaatggc gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg
      181 ctgtgaagat gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc
      241 ttgacactga acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg
      301 ccagtctgca tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg
      361 acccgtgatc cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc
      421 taaagagtaa cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt
      481 gcaatggcat aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt
      541 catagtgatc cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg
      601 gggataacag gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat
      661 gtcggctcat cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta
      721 aagtggtacg cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg
      781 gcgctggaga actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact
      841 ggtgttcggg ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg
      901 ctgaaagcat ctaagcacga aacttgcccc gagatga
//
LOCUS       DADWTH010000313          936 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.321, whole genome shotgun sequence.
ACCESSION   DADWTH010000313 DADWTH010000000
VERSION     DADWTH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.321"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            144..641
                     /locus_tag="JNY02_004375"
                     /pseudo
     CDS             144..641
                     /locus_tag="JNY02_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="JNY02_004376"
                     /pseudo
     CDS             682..873
                     /locus_tag="JNY02_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          244 a          232 c          269 g          191 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DADWTH010000314          922 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.322, whole genome shotgun sequence.
ACCESSION   DADWTH010000314 DADWTH010000000
VERSION     DADWTH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.322"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>922
                     /locus_tag="JNY02_004377"
     CDS             <1..>922
                     /locus_tag="JNY02_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HBB9435598.1"
                     /translation="IELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRMLF
                     GQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSENPIAS
                     KPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTAFK
                     VIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEIY
                     ARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTK
                     TGRLWVYVRDDRNAGSSLPAAVWFAYSADRK"
BASE COUNT          252 a          233 c          248 g          189 t
ORIGIN      
        1 attgagctgc ttaaagccat gttgcgtaag caacagagtc ggcttcgaca gtatgcctgt
       61 caggtcgcgg gctatgagca ggaaattgaa cggctgaaag cgcaactcga caggttgcgt
      121 cgtatgttgt tcggccagag ttcagagaaa aagcgtcata agcttgaaaa tcagatccga
      181 caggcagaaa aacgactgtc ggaactggaa aaccggctga acacagccag aaatcttctg
      241 gaagatgcat cgtcagtcac agattcacct gacaccagtc ccccgtcaga aaacccgatc
      301 gccagtaagc ctgaatcccc gggacgaaaa tcttcacgaa aaccgctgcc ggcagaactt
      361 ccccgggaga cacatcgcct tctgcctgct gaaaccagtt gcccggcctg tggaggtgtt
      421 ctgaaagaaa tgggggaaac aatctcagag caactggata tcattaatac cgcctttaaa
      481 gttatcgaaa ccatacgtcc caaactggcc tgtagccggt gtgatgtcat cgttcaggca
      541 ccacttcccc ctaaaccgat cgaacgcggt tatgccagtg cagggttact tgcacggatc
      601 ctggtcagca aatatatgga acatatccct ttatatcgcc agtcagaaat atacgcgcga
      661 cagggcgtgg agctgagccg taataccatg gtgcgctggg tatcagaaat ggcagacaaa
      721 ctccgtcctc tgtatatagc gctgaatgac tatgttctgg aggcaggaaa ggtgcacgca
      781 gatgacactc cggtgaaagt actggccccg gggaacggaa agacgaaaac gggtcgtctg
      841 tgggtatacg tcagggatga tcgtaatgcg ggttcatccc tgccggcagc cgtctggttc
      901 gcgtattcgg cagatcgcaa ag
//
LOCUS       DADWTH010000315          917 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.323, whole genome shotgun sequence.
ACCESSION   DADWTH010000315 DADWTH010000000
VERSION     DADWTH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.323"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..219)
                     /locus_tag="JNY02_004378"
     CDS             complement(<1..219)
                     /locus_tag="JNY02_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBB9435599.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNL"
     gene            525..>917
                     /locus_tag="JNY02_004379"
     CDS             525..>917
                     /locus_tag="JNY02_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBB9435600.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVA"
BASE COUNT          275 a          209 c          168 g          265 t
ORIGIN      
        1 aagattacga atagtattat ccgcaaaaat atcatcacct actttagtca gcatcaggac
       61 ttttgaattc aatttagccg ccgccaccgc ttgattagca cctttcccac cacatccgat
      121 tttgaaggca ggtgcttcca gagtttctcc ttctttaggc atctgattag tgtaagtaat
      181 gagatccacc atattggaac caataactgc aatgtccatt tcactacctc ttataaactt
      241 tcgcataaca atggtattta aataacatta gcatgttact tttgcatcat ttgtgactga
      301 gatcgcgatt agcacatcaa cccgatgttt atttaataga cttccagtct catcactcag
      361 gccaacacta tctaatcata agcaacctaa caagattagt gcccaaaatt cagcagccta
      421 tacccttttc atttcaaagg ggcggtcgta tagtatggta atgaaaacaa tgtttactaa
      481 cgccaaaatg ttatttttat aacattctta cggagagaga gttgatggaa acgaagcaaa
      541 aagagcgtat ccgacgtttg atggaactgc ttaagaaaac cgacagaatc catttgaaag
      601 acgcagcgcg aatgctggaa gtttctgtaa tgactattcg tcgcgatctc catcaggaag
      661 atgaacctct gccactgacc ctactgggtg gctatattgt aatggtgaat aaacccgcgc
      721 catccatgcc agtaatccat gacgttccaa aaaatcatcg tgatgactta cctattgcaa
      781 ttctggctgc cggaatggtt aatgaaaatg atctgatctt ctttgataat ggccaggaga
      841 taccactcgt tataagcatg atcccggatg caatcacctt caccggcatc tgttactcac
      901 atcgcgtctt tgttgcg
//
LOCUS       DADWTH010000316          902 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.324, whole genome shotgun sequence.
ACCESSION   DADWTH010000316 DADWTH010000000
VERSION     DADWTH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.324"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..216
                     /locus_tag="JNY02_004380"
     CDS             <1..216
                     /locus_tag="JNY02_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435601.1"
                     /translation="GNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTR
                     SEAVLQKSLDITEEADLALRNAMARIK"
     gene            556..>902
                     /locus_tag="JNY02_004381"
     CDS             556..>902
                     /locus_tag="JNY02_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HBB9435602.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDDSSSYQMLP"
BASE COUNT          237 a          225 c          227 g          213 t
ORIGIN      
        1 ggcaacagcg gcggtctgct cagttttaca tcggcacata ccgacgcctg gcgtcagcag
       61 ggctatgaag acacgatgct ggcgatggag catatccgga aaccgctggc agcacgtcag
      121 gcactgaccc ggtcagaggc ggtattgcag aaaagcctgg atataacgga agaggcagat
      181 ttggcactga gaaacgcgat ggcccggatt aaataaagac gccccaaaga aacgccaccg
      241 cctgtggtgg tgtttttcct gtctggtgtt attgaaaata ttttcagata tatattatct
      301 gggtgatggt taagatgaat ttatttaatt ttgggggaag atacaaaaac aaccagtgaa
      361 catatttccg gcgggttatc cgcaatgctg aaggtcagtt gcactggcga tgccggaata
      421 cagcttaata cgtggttcgc cagtgaaggg cttctcagac aataaacctc attcatttca
      481 cccatcaggc cgcgtcttct ccgggagacg cggctttttc atttatatcg cttaatcatt
      541 cataaggagc aaatcatgag attagccagt cgttttggtt atgcgaacca gatacgtcgt
      601 gatcgtccgc tgacacacga agaactgatg cgccatgtac cgagtatttt tggggaaaac
      661 cgacatacct ctcgcagtga acactatgcg tacattccca ctatcaccgt cctggaaaat
      721 ctgcagcagg aaggctttca gccgttcttt gcctgccaga cccgcgtgcg cgaccagagc
      781 cgccgggaat acacaaaaca catgctgcgt ctgcggcggg ccggacagat aaccggtcag
      841 catgtccctg aaattattct gctcaactcc catgacgatt catccagcta ccagatgtta
      901 cc
//
LOCUS       DADWTH010000317          900 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.325, whole genome shotgun sequence.
ACCESSION   DADWTH010000317 DADWTH010000000
VERSION     DADWTH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.325"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            35..900
                     /locus_tag="JNY02_004382"
                     /pseudo
     CDS             35..900
                     /locus_tag="JNY02_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          206 a          234 c          262 g          198 t
ORIGIN      
        1 ttcactggcc ggacggagtc gccccgccct tctgatgctt ccccgttttg ccgatatttt
       61 tcagcaggga aaccgctggc ttaactggct ggagaaacaa ccggaagggg cagtccgtcc
      121 ggtggtaatt gaatcagtga caaaaatcat ggcctgcggg accacactga tgggctacac
      181 gcagtggtgt tgttcatcac cggactgttg ccatacaaaa aaggtctgct tccggtgtaa
      241 aagccgctcc tgcccgcact gcggggtgaa ggctggcgca cagtggatac agtatctgct
      301 gagcctggtc cccgactgcc cgtggcagca tattgtgttc acacttccct gccagtactg
      361 gtccctggtg ttccacaacc ggtggttact ggcagagatg agccgcattg cagcggatgt
      421 gatactggaa atctgccatc aggcagatgt ggagccgggg atattcacgg tgagccacac
      481 atgggggcgt gaccagcagt ggcatccgca tatccattta tcgacaactg ccggtggtgt
      541 gacgtcgggc cacacctgga aaaatcttca tttttacgcc cgtaaggtga tgagcatgtg
      601 gcgctaccgg ataacacggt tgctgtcacg gaaataccct gacctggtga tgccggatgc
      661 gctgacagca gaaggaagca gtaaacggga atggaatcgc ttcctggaca cgcattaccg
      721 ccgtggctga aatgtcaacg tatcccgggt gatggataat gccacccatg tggcggtgta
      781 ctttggctct tacctgaaaa agccaccggt gccgatgagt cgtctggagc attatgccgg
      841 tcaggatgaa atcggtctgc gttacaacag tcaccgaaca aaacgggaag aatacctgtt
//
LOCUS       DADWTH010000318          894 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.326, whole genome shotgun sequence.
ACCESSION   DADWTH010000318 DADWTH010000000
VERSION     DADWTH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.326"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(110..439)
                     /locus_tag="JNY02_004383"
     CDS             complement(110..439)
                     /locus_tag="JNY02_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBB9435603.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..>894)
                     /locus_tag="JNY02_004384"
     CDS             complement(611..>894)
                     /locus_tag="JNY02_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435604.1"
                     /translation="FVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          217 a          207 c          199 g          271 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 ctatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaat
//
LOCUS       DADWTH010000319          881 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.327, whole genome shotgun sequence.
ACCESSION   DADWTH010000319 DADWTH010000000
VERSION     DADWTH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.327"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..691
                     /locus_tag="JNY02_004385"
     CDS             <1..691
                     /locus_tag="JNY02_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9435605.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAM
                     TALLMKDNQRS"
BASE COUNT          198 a          198 c          220 g          265 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctgttag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgttt ggtgccagtt atcgctacag tggtgctgga gtcggttatc aggttgccag
      541 tgtggttggc ggtggattta caccttttat tgccgctgca ctcatcactt actttgccgg
      601 gaactggcat agcatcgcca tttatttgct ggctggatgc ctgatttctg caatgaccgc
      661 tttattgatg aaagacaatc aacgctcgtg atagcctgcc gaagatcatc cgatcttcgc
      721 cttatacttt tgtttcacat tcctgtgaca tactatcgga tgtgcggtaa ttgtatggaa
      781 caggagacac acatgaataa taagggctcc ggtctgaccc cagctcaggc actggataaa
      841 ctcgacgcgc tgtatgagca atctgtagtt gctttacgca a
//
LOCUS       DADWTH010000320          878 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.328, whole genome shotgun sequence.
ACCESSION   DADWTH010000320 DADWTH010000000
VERSION     DADWTH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.328"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>878
                     /locus_tag="JNY02_004386"
     CDS             <1..>878
                     /locus_tag="JNY02_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBB9435606.1"
                     /translation="TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTL
                     WPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTC
                     GGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLV
                     LKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKT
                     GETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEP
                     VAKVCYAQMVKQFLSR"
BASE COUNT          205 a          217 c          286 g          170 t
ORIGIN      
        1 actaactcat gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg
       61 atagcgacac agctgaatcg tcttcctgac tgcgactggg tacacctggt cttcaccctg
      121 ccggacacgc tgtggccggt gttcgaaagc aaccggtggc tgctgaatga cgtgtgccgt
      181 ctggcggtgg agaatctgct gtatgccgcc cggaaacggg ggcaggaacc cggtatcttc
      241 tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc cgcatgtaca tgtgtctgta
      301 acctgtggag gtctgaataa gcatggtcag tggaaaaagc tgagcttcct gaaagacgcg
      361 atgcgttcgc ggtggatgtg gaatatgcgg caacggcttc tgaaagcgtg gtcagagggg
      421 ctggcgatgc cggagtcgtt gtcacatatc acgacggaat cacagaggag aagcctggtg
      481 ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga aaaaaacggc cggcgggagg
      541 aatacggcgc gctacctggg tcgttatctg aagaagccgc cgatagcggc ctcccggctg
      601 gcacattaca acggaggggc gagcctgagc ttccgttacc tggaccacaa aacgggagaa
      661 acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca gcacatcccg
      721 gagaagtttt ttaagatggt gaggtacttc gggttccttg ccagccgggt gtgtggagag
      781 aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt ggcgaaagtg
      841 tgctatgcac aaatggtgaa acagttcctg agtcgtga
//
LOCUS       DADWTH010000321          860 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.329, whole genome shotgun sequence.
ACCESSION   DADWTH010000321 DADWTH010000000
VERSION     DADWTH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.329"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..390
                     /locus_tag="JNY02_004387"
     CDS             <1..390
                     /locus_tag="JNY02_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB9435607.1"
                     /translation="LNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISA
                     SGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVASASLAPISAFDVLIS
                     DRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(392..618)
                     /locus_tag="JNY02_004388"
                     /pseudo
     CDS             complement(392..618)
                     /locus_tag="JNY02_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          225 a          212 c          190 g          233 t
ORIGIN      
        1 ttgaatgaaa agcctaatgt aacagcaata ctttgtggtg gtacgtatcg tgccagaagt
       61 gatgcttttt acgatgccag taactcttcg ccattagact ctctcaatcc gcgaaaaata
      121 tttatttccg ccagcggtgt gcataatcac tttggcgtca gctggtttaa ccctgaagat
      181 cttgccacta agcgtaaagc gatgaaccgt ggactacgga aaattttgct cgcccgccac
      241 gcgttgttcg atgaagtggc ctctgccagc ctcgcaccga tctctgcatt tgacgttctg
      301 attagcgatc gtccgttacc ggcagattat gttacgcact gccagaatgg ttctgtaaag
      361 atcattacac ctgattcaga agacgaatga cttactgaaa aaacaccaca atcttgttaa
      421 acatcgtcgg gttggactga ttacgttgca ctttcaccac atattccagc ttatctattt
      481 ggcttatcac ctactccaga cgctggtcat ccttgaccag tagccagata tggcttttgt
      541 cgcagtcctg aatcggcagg cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag
      601 cccacaaagg tgagtcatta cacccggatg gtaaacaggc acttccttgt tgtagaagcc
      661 gttatattgt ccataatgtc tgggttttta atgcatactg ggtaacaacc ggacgaatct
      721 tcgaatatca ctggcgctgc cagccggttt gaattcatct cacaaccctg catagagcga
      781 atctcctgcc tgcacgtcac tctgaatcgc cacgggttta acagacacct cagagtcatt
      841 taagatggct taaagagagg
//
LOCUS       DADWTH010000322          851 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.330, whole genome shotgun sequence.
ACCESSION   DADWTH010000322 DADWTH010000000
VERSION     DADWTH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.330"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            508..>851
                     /locus_tag="JNY02_004389"
     CDS             508..>851
                     /locus_tag="JNY02_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435608.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYIS"
BASE COUNT          295 a          157 c          172 g          227 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa atgtattacg cgtaacaaac tcatcttctt ctggcataag tgaaaaacat
      661 ttagatcact gtgctaatac tgtgaaaaac tttttaagga agtctatagc agcgcaaagt
      721 tatagcaaaa tgttttctca agggaccagt ttcaaatcac taaatctttc tattgaagcg
      781 ccatctggag cacggtcatc atttcggtca cttgagcatc tcgataaggt gtccaggcac
      841 tatatctctg a
//
LOCUS       DADWTH010000323          832 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.331, whole genome shotgun sequence.
ACCESSION   DADWTH010000323 DADWTH010000000
VERSION     DADWTH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.331"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..211
                     /locus_tag="JNY02_004390"
     CDS             <1..211
                     /locus_tag="JNY02_004390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435609.1"
                     /translation="ADLNIPFFVDARNKKLVFWFVRVDDEGYPEIARCMEREFATIPA
                     GINADGMYCPECGTVHWPDGVIPPF"
     gene            259..688
                     /locus_tag="JNY02_004391"
                     /pseudo
     CDS             259..688
                     /locus_tag="JNY02_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          211 a          168 c          262 g          191 t
ORIGIN      
        1 cgcagacctg aatattccgt tctttgtgga tgccagaaac aaaaaactgg tcttctggtt
       61 tgtacgtgtt gatgacgaag ggtatccgga aattgcccgc tgcatggagc gggaatttgc
      121 gacaattcct gccggtatca acgccgacgg catgtattgc cctgaatgcg gtacggttca
      181 ctggccggat ggcgttattc cgcctttctg atgcttcccc gttttgccga tatttttcag
      241 cagggaaacc gatggcttaa ctggctggag aagcagccgg tgcagatgag ccgcctggaa
      301 cattatgccg gtcaggatga aattggcctg cgttacaaca gccaccggac aaaacgagaa
      361 gaaaaccttg tgatgagtgg cgacgagttt atggaaaggt tctcctggca tgtggcggat
      421 aaggggttcc ggatggtgat acggggtcct gagtccggtg aagcggcgat tactggaaga
      481 tgtggtgtac gccataacgg agacagtgag aaaaacggcg aggcaaatca caaggagagg
      541 gatgtatcag cagtgactga aggttgaccc gctgaagtgc gagctgcgcg gaggtcagat
      601 gcggtttacg gggctgaaga ggggttaccg tctggcagag caggttatga tgcatgagcc
      661 gctggctcga atacggtggt gcggctgaga gtcgcatagg ggaagttgcg tccatgttga
      721 ggggaacgga gcaaaaaacc atcagagata cattgaatca atcaatgtaa gcaatattaa
      781 gtgtctatac cgattacttg atgatcaggt tgtgttgaat aaacgcgcat tc
//
LOCUS       DADWTH010000324          830 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.332, whole genome shotgun sequence.
ACCESSION   DADWTH010000324 DADWTH010000000
VERSION     DADWTH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.332"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..326
                     /locus_tag="JNY02_004392"
     CDS             <1..326
                     /locus_tag="JNY02_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBB9435610.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNY02_004393"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNY02_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBB9435611.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          254 a          181 c          155 g          240 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
      481 atatcgacca ggcgaccttc tgcatgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gggtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagag cttctctaac atgtgttatt attaacaaag aaaaatgtaa
      781 gaagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga
//
LOCUS       DADWTH010000325          795 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.333, whole genome shotgun sequence.
ACCESSION   DADWTH010000325 DADWTH010000000
VERSION     DADWTH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.333"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            101..>795
                     /locus_tag="JNY02_004394"
     CDS             101..>795
                     /locus_tag="JNY02_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBB9435612.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLHELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGG"
BASE COUNT          209 a          205 c          219 g          162 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctcggt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacatg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat aaaaatcaat gagataaccg gttgccagtt
      481 aaatgccgga accgttcgcc gctggttgcc gtctgcgggg attgtgtggc gaagggctgc
      541 gccaactctg cgtatccgtg acccgcataa agatgaaaag atggcagcaa tccataaagc
      601 actggacgaa tgcagcgcag agcatccggt cttttatgaa gatgaagtgg atatccatct
      661 tcatcccaaa atcggtgcgg actggcaact gcgcggacag caaaaacggg tggtcacgcc
      721 gggacagaat gaaaaatatt atctggccgg agcgctgcac agcgggacag gtaaagtcag
      781 ctatgtgggc ggcaa
//
LOCUS       DADWTH010000326          795 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.334, whole genome shotgun sequence.
ACCESSION   DADWTH010000326 DADWTH010000000
VERSION     DADWTH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.334"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..703
                     /locus_tag="JNY02_004395"
                     /pseudo
     CDS             1..703
                     /locus_tag="JNY02_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          211 a          171 c          190 g          223 t
ORIGIN      
        1 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
       61 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      121 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      181 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      241 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      301 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      361 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      421 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      481 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      541 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      601 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
      661 atccatcggt tactcctatt ctaaagcact ggacgtagca ggttggattt gcccctatat
      721 ttccagacac ctgttatcac ttaacccatt actggcccgc tgccgtagat attcccgtgg
      781 cgagcgataa cccag
//
LOCUS       DADWTH010000327          787 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.335, whole genome shotgun sequence.
ACCESSION   DADWTH010000327 DADWTH010000000
VERSION     DADWTH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.335"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..355
                     /locus_tag="JNY02_004396"
                     /pseudo
     CDS             1..355
                     /locus_tag="JNY02_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            368..733
                     /locus_tag="JNY02_004397"
                     /pseudo
     CDS             368..733
                     /locus_tag="JNY02_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT          223 a          167 c          193 g          204 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgttt agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat ggagggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa
      781 ggtgctt
//
LOCUS       DADWTH010000328          756 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.336, whole genome shotgun sequence.
ACCESSION   DADWTH010000328 DADWTH010000000
VERSION     DADWTH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.336"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="JNY02_004398"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="JNY02_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBB9435613.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="JNY02_004399"
     CDS             103..375
                     /locus_tag="JNY02_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB9435614.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            602..>756
                     /gene="ghoS"
                     /locus_tag="JNY02_004400"
     CDS             602..>756
                     /gene="ghoS"
                     /locus_tag="JNY02_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBB9435615.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLN"
BASE COUNT          236 a          128 c          186 g          206 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggc
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 atcaatcggg gttaataagt tttgcgtccc cagagcgttt aatattgata ggagtcatat
      601 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
      661 ttcctcagtg ttcttaagat taagatcatt gatgtatgat atgaatttct cctctatcgt
      721 ggctgatgaa tatgggatac cacgacaatt gaatga
//
LOCUS       DADWTH010000329          750 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.337, whole genome shotgun sequence.
ACCESSION   DADWTH010000329 DADWTH010000000
VERSION     DADWTH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.337"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>750
                     /locus_tag="JNY02_004401"
     CDS             <1..>750
                     /locus_tag="JNY02_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBB9435616.1"
                     /translation="LRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALL
                     RRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDG
                     FEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSS
                     PVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMP
                     KPDGLTAAKNLAEAFEHYNEWHPHSALGYRS"
BASE COUNT          181 a          183 c          230 g          156 t
ORIGIN      
        1 ttctcagacg aaccgatgac tggatggatg gccgccgcag tcgtcacact gatgatacgg
       61 atgtgcttct ccgtatacac catgttatcg gagagctgcc cacgtatggt tatcgtcggg
      121 tatgggcgct gcttcgcaga caggcagaac ttgatggtat gcctgcgatc aatgccaaac
      181 gtgtttaccg gatcatgcgc cagaatgcgc tgttgcttga gcgaaaacct gctgtaccgc
      241 catcgaaacg ggcacataca ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct
      301 ctgacgggtt cgagttctgc tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg
      361 actgctgtga tcgtgaggca ctgcactggg cggtgactac cggcggcttc aacagtgaaa
      421 cagtacagga cgtcatgctg ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt
      481 ctccagtgga gtggctgacg gataatggtt catgctaccg ggctaatgaa acacgccagt
      541 tcgcccggat gttgggactt gaaccgaaga acacggcggt gcggagtccg gagagtaacg
      601 gaatagcaga gagcttcgtg aaaacgataa agcgtgacta catcagtatc atgcccaaac
      661 cagacgggtt aacggcagca aagaaccttg cagaggcgtt cgagcattat aacgaatggc
      721 atccgcatag tgcgctgggt tatcgctcgc
//
LOCUS       DADWTH010000330          712 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.338, whole genome shotgun sequence.
ACCESSION   DADWTH010000330 DADWTH010000000
VERSION     DADWTH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.338"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            278..>712
                     /locus_tag="JNY02_004402"
     CDS             278..>712
                     /locus_tag="JNY02_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9435617.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDVATNVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          217 a          122 c          147 g          226 t
ORIGIN      
        1 atgccaaata taaattttgt gtatcttttt ctgaaaataa actaaagaaa gggctttata
       61 tatcacgcat atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc
      121 agttaatatt taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta
      181 gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag
      241 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
      301 gtttataact gtggtatgtg caacgtccag cgttatggct gctgatgata atgctatcac
      361 ggatggctca gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      421 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      481 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      541 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      601 tgccaaccag gctaccaccg atgtagcgac gaatgttgct ctacaaatgt atctgccaga
      661 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cg
//
LOCUS       DADWTH010000331          693 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.339, whole genome shotgun sequence.
ACCESSION   DADWTH010000331 DADWTH010000000
VERSION     DADWTH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.339"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..>693)
                     /locus_tag="JNY02_004403"
     CDS             complement(1..>693)
                     /locus_tag="JNY02_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB9435618.1"
                     /translation="AALTGTLKTANHLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSD
                     IYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIP
                     DTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCYWPISRLTGWVKIN
                     ITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGSHTEAHDWDRW
                     GFQCPVLPPERI"
BASE COUNT          148 a          191 c          177 g          177 t
ORIGIN      
        1 ctatatacgt tccggtggga gtactgggca ctgaaatccc caccggtccc agtcgtgagc
       61 ctctgtatga ctgcccctga caagtctgat gactttatca ccgtcagctt ctccctggta
      121 cgcagcaata atcagcccgc caatgtcagg gtctccccat ctgttgcgga cagagctcgc
      181 cacgattctg taaataatat cttctgtggt tatatttatt ttcacccagc cagtcagtct
      241 ggatatgggc cagtagcagt agcgggtatg ataaatgggg ccgttaatgc cgtaaaaagt
      301 aagggatttg gctctgtacc gcggttctgt tgtctcaggg cgtgcatcag tccaccggat
      361 gctgagcacc ccttcaaacc gtgtgtcggg tatgatgatg ccgtaagggc cagcaacgga
      421 atattcacct ggcagcgcat tccttaccca ggccaggaaa tcactcttag tgtcaaaacg
      481 gataacatct tcaggcagaa aagcacaccc aaagccgaat gcgccgggta tcgccagacg
      541 gccttttgtc cggtcgtaaa tgtcgctctg tgcttccatc gtggccgcac ttttcagccc
      601 cagattatcc cgggacttct gttgtgcctt ttcgcctgct gctgcgattt cagacagatg
      661 gttagccgtt ttcagggtgc cggtcagcgc agc
//
LOCUS       DADWTH010000332          672 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.340, whole genome shotgun sequence.
ACCESSION   DADWTH010000332 DADWTH010000000
VERSION     DADWTH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.340"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..672
                     /locus_tag="JNY02_004404"
     rRNA            <1..672
                     /locus_tag="JNY02_004404"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          168 a          160 c          201 g          143 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc tt
//
LOCUS       DADWTH010000333          666 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.341, whole genome shotgun sequence.
ACCESSION   DADWTH010000333 DADWTH010000000
VERSION     DADWTH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.341"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..276
                     /locus_tag="JNY02_004405"
     CDS             <1..276
                     /locus_tag="JNY02_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBB9435619.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGFDNGGESAA
                     IIYSLLFTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
     gene            complement(521..>666)
                     /locus_tag="JNY02_004406"
     CDS             complement(521..>666)
                     /locus_tag="JNY02_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435620.1"
                     /translation="NRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQR
                     LRN"
BASE COUNT          159 a          154 c          173 g          180 t
ORIGIN      
        1 ctgaacgtgt actgtcgtaa tggccaggtg gaaatcgaca acaacatcgg tgaaaacgcg
       61 ctgcgatcgg tggcggttgg aagaaaaaat tatctcttct tcggttttga caacggtgga
      121 gaaagcgcgg cgattatcta cagtctgctg ttcacctgca aactgaacgg agtggagccg
      181 gaggactggt tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgagtgcat
      241 gaactgctgc cctggaactt ctcagctgta aaataatcct tccgctacgt acttctctgg
      301 gcgcttacca ctcaacggca tcgtcaaaat tatcaaagcg cagaacatag atttatcatg
      361 tgttttatgg atggctttct ccatcaggca tcgttcgtca cgggaaattg gtgctatgat
      421 cggcattgat cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
      481 tccgggctga gttccctcta agtgatctag gttgtgtccc ttaattacgt aagcgttgat
      541 aaaagagtcg gatgcagccc agtttcacca tcgccaggta atttctcgcc gttttgtcgt
      601 aacgcgtggc gatgcggcga tattctttca gccgcccaaa gcaccgttca acgacattac
      661 gattgc
//
LOCUS       DADWTH010000334          658 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.342, whole genome shotgun sequence.
ACCESSION   DADWTH010000334 DADWTH010000000
VERSION     DADWTH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.342"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(151..657)
                     /locus_tag="JNY02_004407"
     CDS             complement(151..657)
                     /locus_tag="JNY02_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBB9435621.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          173 a          160 c          146 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttggcgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaatc
//
LOCUS       DADWTH010000335          657 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.343, whole genome shotgun sequence.
ACCESSION   DADWTH010000335 DADWTH010000000
VERSION     DADWTH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.343"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            101..>657
                     /locus_tag="JNY02_004408"
     CDS             101..>657
                     /locus_tag="JNY02_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBB9435622.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          108 g          192 t
ORIGIN      
        1 caatacctgg agaaagagta caaagcaaac tgtattaaac ttaataagtt atattgtatt
       61 tcactagagt tcttatcgtt gataagaaat tctggttata atgattaaat caacaaatat
      121 acaggtaatc ggttctggta ttatgcatca aataaacaac atacactcgt taactctatt
      181 ttctttgcct gtgagtttga gtccatcttg taatgaatat tatttaaaag tctggagtga
      241 atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata ggctaaaaat
      301 atgtttgcaa aatcaagggg cggaattaaa tctatctgag ttagatttaa aaacattacc
      361 tgatttaccg cctcagataa caacactgga aataagaaaa aacctattaa catatctccc
      421 tgatttccca ccaatgctta aggtaataca tgctcaattt aatcaactgg aaagcttacc
      481 tgccttaccc gagacgttag aagagcttaa tgtgggtgat aacaagataa aagaattacc
      541 atatcttcct gaaactctaa ctcatttacg gatccataat aaccgattgc atattctgcc
      601 actattgcca ccggagctaa aattactgat agtttctgga aacagattag acagcat
//
LOCUS       DADWTH010000336          636 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.344, whole genome shotgun sequence.
ACCESSION   DADWTH010000336 DADWTH010000000
VERSION     DADWTH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.344"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            88..153
                     /locus_tag="JNY02_004409"
                     /pseudo
     CDS             88..153
                     /locus_tag="JNY02_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JNY02_004410"
     CDS             381..629
                     /locus_tag="JNY02_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBB9435623.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DADWTH010000337          632 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.345, whole genome shotgun sequence.
ACCESSION   DADWTH010000337 DADWTH010000000
VERSION     DADWTH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.345"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            66..281
                     /locus_tag="JNY02_004411"
     CDS             66..281
                     /locus_tag="JNY02_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBB9435624.1"
                     /translation="MYQMEKISTGIAYGTSGGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            281..>632
                     /locus_tag="JNY02_004412"
     CDS             281..>632
                     /locus_tag="JNY02_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBB9435625.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVTRQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTS"
BASE COUNT          162 a          136 c          171 g          163 t
ORIGIN      
        1 catcagagtt ttgctgatgg ccttttttct ttccgatagc acaggtctgt cggggggggc
       61 gggatatgta tcagatggaa aaaatatcaa caggcattgc ctacggcacc tccggcggca
      121 gtgccggcta ctggtttttg cagtggttgg atcaggtcag tccatcacag tgggctgcga
      181 ttggtgtgct gggaagtctg cttctggggc ttctgactta tctgacgaat ctgtatttca
      241 aaataagaga agataagcgt aaggctgcga gaggtgaata atgtcgccat cattacgcaa
      301 ggctgttgca gttgctattg gtggcggggc tgttgctata gcatctgtgt taatcactgg
      361 cccaggtggt aacgatggtc tggaaggtgt cagctacata ccatacaaag atatcattgg
      421 tgtatggact gtatgtcacg gacacaccgg aaaagacatc atgcctggta aaacgtatac
      481 cgaagcagaa tgcaaagctc tcctgaataa agaccttgtc actgtcacca gacaaattaa
      541 cccgtacatc aaagtcgata taccggaaac aacgcgcggc gctctttact cgttcgttta
      601 caacgtgggc gctggcaatt tcagaacatc ga
//
LOCUS       DADWTH010000338          615 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.346, whole genome shotgun sequence.
ACCESSION   DADWTH010000338 DADWTH010000000
VERSION     DADWTH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.346"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="JNY02_004413"
     CDS             complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="JNY02_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HBB9435626.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSYFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          187 a          122 c           91 g          215 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaatta tatacaaaaa ataactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgtt
//
LOCUS       DADWTH010000339          589 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.347, whole genome shotgun sequence.
ACCESSION   DADWTH010000339 DADWTH010000000
VERSION     DADWTH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.347"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(273..>589)
                     /locus_tag="JNY02_004414"
     CDS             complement(273..>589)
                     /locus_tag="JNY02_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBB9435627.1"
                     /translation="KKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRF
                     EI"
BASE COUNT          172 a          121 c           97 g          199 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtgcg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattatca
      361 agcatagtgt ccccactatt tgctttttga aattttttgt ttacatcaac caatccatta
      421 ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa gtattcaaca
      481 tcatacagat tacaatatct attgttgttc attttctcca tctctgcgac atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gctatatcag cttttttat
//
LOCUS       DADWTH010000340          566 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.348, whole genome shotgun sequence.
ACCESSION   DADWTH010000340 DADWTH010000000
VERSION     DADWTH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.348"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..297
                     /locus_tag="JNY02_004415"
     CDS             <1..297
                     /locus_tag="JNY02_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBB9435628.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JNY02_004416"
     CDS             complement(405..>566)
                     /locus_tag="JNY02_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435629.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADWTH010000341          561 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.349, whole genome shotgun sequence.
ACCESSION   DADWTH010000341 DADWTH010000000
VERSION     DADWTH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.349"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            283..561
                     /locus_tag="JNY02_004417"
                     /pseudo
     CDS             283..561
                     /locus_tag="JNY02_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          149 a          156 c          125 g          131 t
ORIGIN      
        1 ccctcgcgat attaacgtaa tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga
       61 tcacggcagc agacaggtaa aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt
      121 atgataaatt ttaaccacat gcatacctgt gcagcaaggc gaatcacgca cggatttcca
      181 gccagagaca cctgaaacgt aaccggctca tttaaaccgt ctggtctgtt acctccggtt
      241 ccacaaaaat aatgtccatc attttgaagt ggacactatc gtatggaata ccggacctga
      301 atcactgaag ctttacgcct tcatttcgaa gaacatttac cacgggttgt ggccggacgt
      361 cgcctgggcg taccaaaatc aactgtctgt ggtatgttcg tgcgcttccg caacgctggc
      421 ctttcatggc ctctgcccgc aggtatgtcg gagcaggaac ttgatgccct tctttacgga
      481 agtgcctcca cagtacctgt cgtacttact gaaagcaccg ttatgcccaa actcccggtg
      541 gtaaaaaaac gtccccggcg g
//
LOCUS       DADWTH010000342          553 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.350, whole genome shotgun sequence.
ACCESSION   DADWTH010000342 DADWTH010000000
VERSION     DADWTH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.350"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(28..>553)
                     /locus_tag="JNY02_004418"
     CDS             complement(28..>553)
                     /locus_tag="JNY02_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBB9435630.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGAAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT          144 a          139 c          134 g          136 t
ORIGIN      
        1 accagtattt cttcggagtg aagaatatta ccaagtatat ttaacaccca cgttcgcgga
       61 ccagtcttga tctacgtcac caccaccgag gtagttagca tcggtatagg cgctgaagtt
      121 cttggtgaag ctgaactgag tacccagacc aacacgtacc gcagcccctt cagtaccgtt
      181 atcgatggaa tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag
      241 tttgaagtac ggagtcagag cctgatcttc gctgtaggtg aaggtataac ctgcatctac
      301 acccagttca taacgcatgc tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag
      361 ctggtagtca tcaccagact ggaacagacc agaaatgctg ccgtaaggag tcacgtaacc
      421 agcatcaccc agtttgaagt cgtaaccggc tttcaaaccg aagccccaag cgtcggagtt
      481 ggtgctaccg tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg
      541 agagtagctc aag
//
LOCUS       DADWTH010000343          543 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.351, whole genome shotgun sequence.
ACCESSION   DADWTH010000343 DADWTH010000000
VERSION     DADWTH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.351"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(88..237)
                     /gene="hokB"
                     /locus_tag="JNY02_004419"
     CDS             complement(88..237)
                     /gene="hokB"
                     /locus_tag="JNY02_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBB9435631.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(337..477)
                     /locus_tag="JNY02_004420"
                     /pseudo
     CDS             complement(337..477)
                     /locus_tag="JNY02_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          141 a          127 c          126 g          149 t
ORIGIN      
        1 aacatgcacg aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat
       61 gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac
      121 ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa
      181 tgccagaatc gtaatgcaga taatgagcag acacaccacc agagggttgc gcttcatagc
      241 ctttctcctt gccggatggc gggtaagagg ctaacatctg aattgcttgg ttcattcgtt
      301 ggcctcggtt tgtactgacc ccaaaaagtt ggacagttaa acacgaggca tataggtctg
      361 attccgatat tcatttggag tcagaccttt taatttcagg ctaattcttc tgctgttgta
      421 gtattcaata tattccgtaa cagcatcctt cagttcgctt atattactga actcattaga
      481 ctggccccct gaatctccag acaaccagta tcacttattt aagtgatagt cttaatacta
      541 gtt
//
LOCUS       DADWTH010000344          523 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.352, whole genome shotgun sequence.
ACCESSION   DADWTH010000344 DADWTH010000000
VERSION     DADWTH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.352"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>523)
                     /locus_tag="JNY02_004421"
     CDS             complement(<1..>523)
                     /locus_tag="JNY02_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBB9435632.1"
                     /translation="CADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVV
                     DVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTI
                     TVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDVA
                     LAVVAHPNADNAMQ"
BASE COUNT          148 a          124 c          105 g          146 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc aca
//
LOCUS       DADWTH010000345          522 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.353, whole genome shotgun sequence.
ACCESSION   DADWTH010000345 DADWTH010000000
VERSION     DADWTH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.353"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..234
                     /locus_tag="JNY02_004422"
                     /pseudo
     CDS             1..234
                     /locus_tag="JNY02_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(260..>522)
                     /locus_tag="JNY02_004423"
     CDS             complement(260..>522)
                     /locus_tag="JNY02_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HBB9435633.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          130 a          125 c          123 g          144 t
ORIGIN      
        1 cgaatgttgt acatcgaatt cactgacaat atgcgttatg acacgctgga gacctgccat
       61 cataatgcgt tccgcttctt tggtggtgtg ccgcgcgaag tgttgtatga caatatgaaa
      121 actgtggttc tgcaacgtga cgcatatcag accggtcagc accggttcca tccttcgctg
      181 tggcagttcg gcaaggagat gggcttctct ccccgactgt gtcgcccctt caggctgagg
      241 gatccccaca aaattactgc taataaacca gcaccatatt tcggtaggtt ctggacagat
      301 ggtttagaac tgtgctcagt actgttcgtc ttaaaataag tggcgagtgt cgaagaacat
      361 tcttcgctaa cgtcagatat gagataaccc gccgggattt aatgctgtta gcctgatatt
      421 tcaggtgtaa ccctttattt tcagcgtgat aaccgagtaa ccacattacg atagtgctca
      481 acgttgccag cagactcaga accagaattc ttcctgttga ac
//
LOCUS       DADWTH010000346          521 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.354, whole genome shotgun sequence.
ACCESSION   DADWTH010000346 DADWTH010000000
VERSION     DADWTH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.354"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT          119 a          128 c          143 g          131 t
ORIGIN      
        1 acccgattag ttgttaatca tatttaatca tagtgattac tttcagacgg aacaaggcaa
       61 ataatgcgag tcagcgcata taaggcggca tacggtggtt acatgtagca caggtgtaaa
      121 accgccgaag cccggcgcaa gccggtacgg tcgtccgaat atcaccagtc cccctccggc
      181 tctgcgaggc catgcggcct gtcagcgatg aaaatgcctc tgtgcgcggt cggagtggtc
      241 ccgacaaggg tttacccgca gtcggggcgt atctccgcgt tagcgggccg tgagggccgc
      301 ttacgagcgt gtatgagaga cgtacagtag ttttgtccgc tcacaccagt acaaaccagt
      361 cgaaataccg gtaacgacga ttactcttgt ctctgatgcc ggtggactac gagattttat
      421 ttactggcgt cctgatgcgg tcaggactgg tatggaacct gtttatgtga tacttaatgc
      481 tcttctggat tccgggcgat ttacgcgtaa acttattctg t
//
LOCUS       DADWTH010000347          514 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.355, whole genome shotgun sequence.
ACCESSION   DADWTH010000347 DADWTH010000000
VERSION     DADWTH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.355"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>514)
                     /locus_tag="JNY02_004424"
     CDS             complement(<1..>514)
                     /locus_tag="JNY02_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB9435634.1"
                     /translation="REGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVL
                     QRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENML
                     DQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFG
                     TLKSECFYLD"
BASE COUNT          133 a          121 c          102 g          158 t
ORIGIN      
        1 tcatcaagat aaaaacactc cgactttaag gttccaaaga aacactccac cacagcatta
       61 tccagacaat tgccttttct ggacatgctt tgtttaatac catgttcttt aaggatattt
      121 tgatatcttc tcatacgata ctgccatccc tggtcagagt gcagaacagg atgctcgtga
      181 ggattaagct ttttgaatgc ctgatcgagc atattctcaa ccatgttcat cactggtctt
      241 tccgaaaggc tgtaagaaat aacttcgttg ttgaagagat ctattactgg agacaaatac
      301 agcttgcgcc cattgactgc aaattcagta acatcggtaa cccacttctc gtttggccgc
      361 gtagccttga aatctctttg gagaacatta ggggcggttt gccctacctc tcctctgtaa
      421 gagcggtatc gcttgacctt aatcgctgct ttaagtgaga gggttcccat caggcgctga
      481 acagctttat ggttaatctg tttcccttct cgat
//
LOCUS       DADWTH010000348          505 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.356, whole genome shotgun sequence.
ACCESSION   DADWTH010000348 DADWTH010000000
VERSION     DADWTH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.356"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..483
                     /gene="istB"
                     /locus_tag="JNY02_004425"
     CDS             <1..483
                     /gene="istB"
                     /locus_tag="JNY02_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBB9435635.1"
                     /translation="ERNENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAAELLL
                     QLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMIL
                     TSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE
                     "
BASE COUNT          147 a          121 c          128 g          109 t
ORIGIN      
        1 gaacgtaatg aaaatatcgt attactgggg ccatcaggtg tggggaaaac ccatctggca
       61 atagcgatgg gctatgaagc agtccgtgca ggtatcaaag ttcgcttcac aacagcagca
      121 gagctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac gacgcttcag
      181 cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct gccgttcagt
      241 caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa gagcgcaatg
      301 atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg tgatgcagca
      361 ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca aatcaaagga
      421 gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc taatcctgag
      481 taaaacggtg gatcaatatt gggcc
//
LOCUS       DADWTH010000349          495 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.357, whole genome shotgun sequence.
ACCESSION   DADWTH010000349 DADWTH010000000
VERSION     DADWTH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.357"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>495)
                     /locus_tag="JNY02_004426"
     rRNA            complement(<1..>495)
                     /locus_tag="JNY02_004426"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DADWTH010000350          477 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.358, whole genome shotgun sequence.
ACCESSION   DADWTH010000350 DADWTH010000000
VERSION     DADWTH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.358"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(164..>477)
                     /locus_tag="JNY02_004427"
     CDS             complement(164..>477)
                     /locus_tag="JNY02_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBB9435636.1"
                     /translation="EQEAKPFTFEWNGRIWNAGPNSLGRLSPVVMLAKSVAAQTNMTW
                     SDADNQQVQLTTQELEELATAMIQAIVERNDEIYRRQREMKEELSGLDDLASIRAFDV
                     E"
BASE COUNT           96 a          157 c          111 g          113 t
ORIGIN      
        1 acagaccgga ctgactccgg aaaaacggag gcccgccccc gggcctcccc tgattcatcc
       61 gtttccctgt tcagcctgac agcacgcccc ggctgccgga tgacagactc cgcttcggta
      121 agcaaagcgg tcttctgtga ttccgccagt tgcggcttat tcattactca acgtcaaacg
      181 cccgaattga agccaaatca tccagaccgc tcagctcctc tttcatttca cgctgacggc
      241 gataaatctc atcgttgcgc tcaacaatgg cctgtatcat cgctgtggcc agttcctcaa
      301 gttcctgcgt cgtcagctgc acctgctgat tatccgcatc actccacgtc atatttgttt
      361 gtgccgcgac agattttgcc agcatgacca ccggggacag gcggcccagt gagttggggc
      421 cagcattcca gatacgaccg ttccattcaa acgtgaacgg cttcgcctcc tgttctg
//
LOCUS       DADWTH010000351          475 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.359, whole genome shotgun sequence.
ACCESSION   DADWTH010000351 DADWTH010000000
VERSION     DADWTH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.359"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            54..>475
                     /gene="istA"
                     /locus_tag="JNY02_004428"
     CDS             54..>475
                     /gene="istA"
                     /locus_tag="JNY02_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HBB9435637.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHV"
BASE COUNT          117 a          123 c          123 g          112 t
ORIGIN      
        1 acgcctgaaa tcagatccag ggggtaatct gctctcctga ttcaggagag cttatggtca
       61 cttttgagac agttatggaa attaaaatcc tgcacaagca gggaatgagt agccgggcga
      121 ttgccagaga actggggctc tcccgcaata ccgttaaacg ttatttgcag gcaaaatctg
      181 agccgccaaa atatacgccg cgacctgctg ttgcttcact cctggatgaa taccgggatt
      241 atattcgtca acgcatcgcc gatgctcatc cttacaaaat cccggcaacg gtaatcgctc
      301 gcgagatcag agaccaggga tatcgtggcg gaatgaccat tctcagggca ttcattcgtt
      361 ctctctcggt tcctcaggag caggagcctg ccgttcggtt cgaaactgaa cccggacgac
      421 agatgcaggt tgactggggc actatgcgta atggtcgctc accgcttcac gtgtt
//
LOCUS       DADWTH010000352          473 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.360, whole genome shotgun sequence.
ACCESSION   DADWTH010000352 DADWTH010000000
VERSION     DADWTH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.360"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..410)
                     /locus_tag="JNY02_004429"
     CDS             complement(<1..410)
                     /locus_tag="JNY02_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HBB9435638.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          110 a          117 c           94 g          152 t
ORIGIN      
        1 tcatgttcga acaccactcc aacatctgtt tgaacacctg ttaaatttct ctcttgtttt
       61 gtttgaacat ctgcttcctt tctgcttctt ctggcctgaa cagatgcttt tcctgcggct
      121 gattttttgg ttaatttttc tctgacagat gccagatctt cctcaatccg aagatgcatc
      181 cattcgtcgc cgttatcaca aaaaaactcc cgcaaggatg gttcaacatc aacccatcgc
      241 tcgttagtca gacgggcaat ttttgccaac ctgtttttgg gtattggctt tcctgtttgc
      301 cagtaattga acatcagcaa caaatacgcg ccgtgctcct ctgctgacaa atgcatggtg
      361 tcagccagat aatcagctat gtacagttgc atgtatggta atgcggccat aattgccccg
      421 tatgatgcta cccggttgct tagaataagt gaggtagcct gagtttaacg gac
//
LOCUS       DADWTH010000353          464 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.361, whole genome shotgun sequence.
ACCESSION   DADWTH010000353 DADWTH010000000
VERSION     DADWTH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.361"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>464
                     /locus_tag="JNY02_004430"
     CDS             <1..>464
                     /locus_tag="JNY02_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBB9435639.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DADWTH010000354          463 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.362, whole genome shotgun sequence.
ACCESSION   DADWTH010000354 DADWTH010000000
VERSION     DADWTH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.362"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>463
                     /locus_tag="JNY02_004431"
     CDS             <1..>463
                     /locus_tag="JNY02_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HBB9435640.1"
                     /translation="KRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLA
                     VVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQ
                     LLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT          111 a          111 c          146 g           95 t
ORIGIN      
        1 ataaacgtgg tggtcatgaa catgttgcta tccctaacta ccttgaaagg cagttcgccg
       61 tgaccgagcc aaatcaggtg tggtgcggtg atgtgaccta tatctggacg ggtaagcgct
      121 gggcgtacct cgccgttgtt ctcgacctgt tcgcaagaaa accagtgggc tgggccatgt
      181 cgttctcgcc ggacagcagg ctcaccatga aagcgctgga aatggcatgg gaaacccgtg
      241 gtaagcccgg cggggtgatg ttccacagcg atcagggcag tcattatacg agcaggcagt
      301 tccggcagtt attgtggcga taccagatca gacagagtat gagccggcgc ggaaactgct
      361 gggataacag cccaatggaa cgcttcttca ggagtctgaa gaacgaatgg atgccggtgg
      421 tgggttacgt aagcttcagc gaggcagctc acgccataac gga
//
LOCUS       DADWTH010000355          462 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.363, whole genome shotgun sequence.
ACCESSION   DADWTH010000355 DADWTH010000000
VERSION     DADWTH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.363"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..183
                     /locus_tag="JNY02_004432"
     CDS             <1..183
                     /locus_tag="JNY02_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435641.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            278..>462
                     /locus_tag="JNY02_004433"
     CDS             278..>462
                     /locus_tag="JNY02_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBB9435642.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRG"
BASE COUNT          137 a           91 c          136 g           98 t
ORIGIN      
        1 caactcgaaa aaacgggcat aacaaaagat gctatccgta aagatatggt acccgcgctt
       61 cgggaactga tgcaggcgaa gcatcgcgca cagaaagcag tagatgagct ggtggactgt
      121 gtggcagagc tggaaaccag agtcggaaag tttgaaaaac tggtgcagga ggcgctgcgc
      181 tgatgcgcca tgagtttgtt ttgccctggc ttgtgcgcga tcattatgtc cctggtggaa
      241 agcccgggat aaaaatcacc gaaccggaga gtatttgatg agcgtcaaaa ttcaaacgat
      301 accggaactg cttatccaga cacgaggtaa tatgaccgaa gtttcacgaa tgctgaactg
      361 taatcgcgcc acggtaagaa aatatgctga agataaagag ggcaaagggc acgccattgt
      421 tgatggtgtt ctgattgttc atcgtggatg ggacaggggt aa
//
LOCUS       DADWTH010000356          453 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.364, whole genome shotgun sequence.
ACCESSION   DADWTH010000356 DADWTH010000000
VERSION     DADWTH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.364"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..384
                     /locus_tag="JNY02_004434"
     CDS             <1..384
                     /locus_tag="JNY02_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBB9435643.1"
                     /translation="RCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
BASE COUNT          103 a           98 c          157 g           95 t
ORIGIN      
        1 cgttgttatg tggcacttga agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag
       61 ggcgaaacag gccaggttaa ccagctactg gatattctca gacataaagc attaacgcag
      121 atggcacagg agagtggcgg tagtgcaacc gtgcgtctga acactctgga ctggcttggt
      181 ggtcagggcc gggagcaggc agataacgag tggcatgacg cgattaactg gctgggagac
      241 tggtgcagtg aagagcagca tccggtgatc tggtcaacca cgcaggctgc agaacactta
      301 ccggttcgca tgcctcgcct ctgctctgca gaacgcctct ctgaaagtat ggtcgatgaa
      361 atatttcaga agggggctgc atgagtacag aaatgaaaac ggggctggtg ctgtccggtg
      421 gcggggcggt gggcgcttat caggcgggag tgg
//
LOCUS       DADWTH010000357          441 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.365, whole genome shotgun sequence.
ACCESSION   DADWTH010000357 DADWTH010000000
VERSION     DADWTH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.365"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            211..>441
                     /locus_tag="JNY02_004435"
     CDS             211..>441
                     /locus_tag="JNY02_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBB9435644.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          139 a          105 c           62 g          135 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccttggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatgg a
//
LOCUS       DADWTH010000358          439 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.366, whole genome shotgun sequence.
ACCESSION   DADWTH010000358 DADWTH010000000
VERSION     DADWTH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.366"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..439
                     /locus_tag="JNY02_004436"
                     /pseudo
     CDS             71..439
                     /locus_tag="JNY02_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a          100 c          119 g           96 t
ORIGIN      
        1 cagacaacca gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagct
//
LOCUS       DADWTH010000359          432 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.367, whole genome shotgun sequence.
ACCESSION   DADWTH010000359 DADWTH010000000
VERSION     DADWTH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.367"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>432
                     /locus_tag="JNY02_004437"
     CDS             <1..>432
                     /locus_tag="JNY02_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435645.1"
                     /translation="LQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQ
                     TVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRT
                     AARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRDEHP"
BASE COUNT           84 a          147 c          114 g           87 t
ORIGIN      
        1 ttctccagcg atatccctca ccagaaaaac tcgcttcgct gggtgagaag aagctggcag
       61 cccagctctg caaacttgcg cctcgtctgg gtaaacgcct tgcagcagac atagctcagg
      121 cactggccga acaaaccgtc gtcgttcccg gcacgaatgc cgctgccgta gtactgccac
      181 gtctggcact ccagctcatc acgctgcgta agcaaagaga cgaggtggcg cttgaggtag
      241 aacagcgagt tcttgctcac cctctttacc cggtcctgac cagtatgccc ggagtcggtg
      301 tcaggaccgc agccagactc ctcaccgagg tcgcctgccg cgccttcgcc tctgccgcac
      361 atctcgctgc ttatgctggc cttgcgccgg taactcggcg atccggctcg tcaatacgcg
      421 atgagcatcc ct
//
LOCUS       DADWTH010000360          427 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.368, whole genome shotgun sequence.
ACCESSION   DADWTH010000360 DADWTH010000000
VERSION     DADWTH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.368"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(9..218)
                     /locus_tag="JNY02_004438"
                     /pseudo
     CDS             complement(9..218)
                     /locus_tag="JNY02_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(268..>427)
                     /locus_tag="JNY02_004439"
     CDS             complement(268..>427)
                     /locus_tag="JNY02_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9435646.1"
                     /translation="YIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRE
                     RSLEFEKE"
BASE COUNT          130 a          108 c           78 g          111 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gagaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatatag
//
LOCUS       DADWTH010000361          395 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.369, whole genome shotgun sequence.
ACCESSION   DADWTH010000361 DADWTH010000000
VERSION     DADWTH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.369"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..333
                     /locus_tag="JNY02_004440"
     CDS             <1..333
                     /locus_tag="JNY02_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435647.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETASRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPPQHHPLSIY
                     DSFCRGVA"
     gene            330..>395
                     /locus_tag="JNY02_004441"
     CDS             330..>395
                     /locus_tag="JNY02_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435648.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
BASE COUNT           98 a          114 c          106 g           77 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccagccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaatct ggtgagcttc gacaaccccc cccagcatca tccactctcc
      301 atctacgact cattctgcag aggagtggcg tgatgatgga actgcaacat caacgactga
      361 tggcgctcgc cgggcagttg caactggaaa gcctt
//
LOCUS       DADWTH010000362          368 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.370, whole genome shotgun sequence.
ACCESSION   DADWTH010000362 DADWTH010000000
VERSION     DADWTH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.370"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..222
                     /locus_tag="JNY02_004442"
     CDS             <1..222
                     /locus_tag="JNY02_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBB9435649.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            268..>368
                     /locus_tag="JNY02_004443"
     CDS             268..>368
                     /locus_tag="JNY02_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB9435650.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLS"
BASE COUNT          113 a           79 c           88 g           88 t
ORIGIN      
        1 tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggaatgaat
       61 attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta caacacagat
      121 cccgtgctac ctgtccttca gaacgaagcc agacaacaaa aacgtgggct ctggtcagat
      181 gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg caatcctatc
      241 attcatctgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg gctgtatcag
      301 ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa cagagacagt
      361 ttatcaaa
//
LOCUS       DADWTH010000363          351 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.371, whole genome shotgun sequence.
ACCESSION   DADWTH010000363 DADWTH010000000
VERSION     DADWTH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.371"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..230)
                     /locus_tag="JNY02_004444"
     CDS             complement(<1..230)
                     /locus_tag="JNY02_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBB9435651.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHL"
BASE COUNT          128 a           55 c           53 g          115 t
ORIGIN      
        1 aggtgaatag tgaaaggttc agccaaagat ttctgatcgc ttttcagtac ggtatctgct
       61 acttcaccca gatttacagt taaatcttcg tcacctggtt taatagaaca tggagcagag
      121 ataacttcac ctttaaattt aacaataccg tgaccttcat cagcaaacac gttattggaa
      181 agaatcattg ctacaccagc aacaagaagt gactttttaa aagcagacat aatattcctt
      241 tatataaaga taagtaaaaa agagaatgat tagtaactgt tatttaagga ctaccaatca
      301 ttaaaatatt atattttgtt ttgtgatttt tataaaatta gtttatctaa a
//
LOCUS       DADWTH010000364          351 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.372, whole genome shotgun sequence.
ACCESSION   DADWTH010000364 DADWTH010000000
VERSION     DADWTH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.372"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..208)
                     /locus_tag="JNY02_004445"
     CDS             complement(<1..208)
                     /locus_tag="JNY02_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HBB9435652.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="JNY02_004446"
     CDS             complement(205..>351)
                     /locus_tag="JNY02_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HBB9435653.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DADWTH010000365          345 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.373, whole genome shotgun sequence.
ACCESSION   DADWTH010000365 DADWTH010000000
VERSION     DADWTH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.373"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="JNY02_004447"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="JNY02_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBB9435654.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DADWTH010000366          341 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.374, whole genome shotgun sequence.
ACCESSION   DADWTH010000366 DADWTH010000000
VERSION     DADWTH010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.374"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>341
                     /gene="rrrD"
                     /locus_tag="JNY02_004448"
     CDS             <1..>341
                     /gene="rrrD"
                     /locus_tag="JNY02_004448"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBB9435655.1"
                     /translation="LRKAVAVAIGGGAIAIASVLITGPGGNDGLEGVSYIPYKDIVGV
                     WTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGALYSFV
                     YNVGAGNFRTS"
BASE COUNT           94 a           84 c           85 g           78 t
ORIGIN      
        1 attacgcaag gctgttgcag ttgctattgg tggtggggct attgccatag cgtctgtgct
       61 catcactggc ccaggtggta acgatggtct ggaaggtgtc agctacatac catacaaaga
      121 tatcgttggc gtatggactg tatgccacgg acacaccgga aaagacatca tgctcggtaa
      181 aacgtatacc gaagcagaat gcaaagccct cctgaataaa gaccttgcca ctgtcgccag
      241 acaaattaac ccgtacatca aagtcgatat accggaaaca acgcgcggcg ctctttactc
      301 gttcgtttac aacgtgggcg ctggcaattt cagaacatcg a
//
LOCUS       DADWTH010000367          337 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.375, whole genome shotgun sequence.
ACCESSION   DADWTH010000367 DADWTH010000000
VERSION     DADWTH010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.375"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>337
                     /locus_tag="JNY02_004449"
     CDS             <1..>337
                     /locus_tag="JNY02_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435656.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERP"
BASE COUNT          100 a           59 c          108 g           70 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggcc
//
LOCUS       DADWTH010000368          326 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.376, whole genome shotgun sequence.
ACCESSION   DADWTH010000368 DADWTH010000000
VERSION     DADWTH010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.376"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT           94 a           80 c           62 g           90 t
ORIGIN      
        1 ttgtatgaaa aaacacgcac gactaattga caataatttt catttccatt aaaaaagatc
       61 gcgtagcatt cttaattcat gaggaacttt acccgccagc aatctgagta gcgaaagctg
      121 ttcatcccca atgtttttgc tggcgggtcc ttttttctac ggtcctcctc cccggagggg
      181 ggcatttcat tatttttcta atcgttactg gtgagaaggc ggaagaaaaa gccagttctt
      241 cgcccctaaa taacagcatg attccagtcc atgcaaaatc tgatttccag ctcacacata
      301 ccgagcgctg tacaaaaaac cgccag
//
LOCUS       DADWTH010000369          325 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.377, whole genome shotgun sequence.
ACCESSION   DADWTH010000369 DADWTH010000000
VERSION     DADWTH010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.377"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..325)
                     /locus_tag="JNY02_004450"
                     /pseudo
     CDS             complement(1..325)
                     /locus_tag="JNY02_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           94 a           79 c           53 g           99 t
ORIGIN      
        1 ggctgtacgc ttccccatgt gatttgcctc gccacttcac cgggctcagg accccgtagg
       61 atcttcaccg aaaaaaatgc atcaccagtt gctttattat ttgttttagg tagacctgtt
      121 actaatttat acctactctg accaagtctg gataagaaag gcacacctgc acatttctac
      181 ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata ttcctcgtac
      241 agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt catatattac
      301 tcgaccttgt tgagatacaa caaca
//
LOCUS       DADWTH010000370          304 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.378, whole genome shotgun sequence.
ACCESSION   DADWTH010000370 DADWTH010000000
VERSION     DADWTH010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.378"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            149..304
                     /locus_tag="JNY02_004451"
                     /pseudo
     CDS             149..304
                     /locus_tag="JNY02_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT           84 a           77 c           79 g           64 t
ORIGIN      
        1 cggtggtcgg tttacgctta ccgcaggaca ggtggtaact gaaaaattgc tgaggcgact
       61 ggaaacagaa gggcaagatc gattccgaca gcagtgatgg tcagctacct catcgataac
      121 tttgcaacag tgcccttctt gaagcatcat gcttcatgaa gaaaaactgg cacgtcatca
      181 acgtaaacag gcgatgtata cccgaatggt agccttcccg gcggtgaaaa tgttcgaaga
      241 gtatgacttc acgttcgcca ccggagcacc gcagaagcag ctccagtcgt tacgctcact
      301 cagc
//
LOCUS       DADWTH010000371          302 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.379, whole genome shotgun sequence.
ACCESSION   DADWTH010000371 DADWTH010000000
VERSION     DADWTH010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.379"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT           99 a           52 c           70 g           81 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 at
//
LOCUS       DADWTH010000372          302 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.380, whole genome shotgun sequence.
ACCESSION   DADWTH010000372 DADWTH010000000
VERSION     DADWTH010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.380"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>302
                     /locus_tag="JNY02_004452"
     CDS             <1..>302
                     /locus_tag="JNY02_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435657.1"
                     /translation="ARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDT
                     PEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE"
BASE COUNT           80 a           81 c           80 g           61 t
ORIGIN      
        1 ttgcccggat cgccagagaa cacgatatca ataataacct gctgtttaaa tggaaaagcc
       61 agtacgagga cggcttactg agcgatgatg acatacagga atgcatgcct gtcccggtgg
      121 cactgactga tacgccggag ccgaccagac cagttacaaa tcccttctgg cgtaacaagc
      181 atgatgagcg ccctgagggg gctcccggaa acgtcccacg gtgcgagctg catcttaaat
      241 caggtgtggt aaaactgttt gaccctctca ctccggaact gttacgggcg ctaatccgcg
      301 aa
//
LOCUS       DADWTH010000373          286 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.381, whole genome shotgun sequence.
ACCESSION   DADWTH010000373 DADWTH010000000
VERSION     DADWTH010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.381"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>286
                     /locus_tag="JNY02_004453"
     CDS             <1..>286
                     /locus_tag="JNY02_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBB9435658.1"
                     /translation="SLIDQLITEVILSSHSRAAALAVDSAAAKLMQNADNISEYLSLH
                     HQGLMQSIQSLQAHITSLLEDIREIADCQEQVTADVRMAMEEIDARTRELL"
BASE COUNT           81 a           69 c           76 g           60 t
ORIGIN      
        1 tcacttatcg atcaactgat aacggaagtc atactgagtt cacactccag agctgcggca
       61 ctggcggttg attctgccgc cgcaaaactg atgcagaatg cagacaatat cagtgaatac
      121 ctgtcgttac accatcaggg gctgatgcaa agtattcagt cactgcaggc gcatatcacc
      181 agcctgcttg aggatatccg ggaaatagca gactgtcagg agcaggtgac cgctgatgtc
      241 agaatggcca tggaggagat cgatgccaga acccgggaat tactga
//
LOCUS       DADWTH010000374          279 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.382, whole genome shotgun sequence.
ACCESSION   DADWTH010000374 DADWTH010000000
VERSION     DADWTH010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.382"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..279
                     /locus_tag="JNY02_004454"
                     /pseudo
     CDS             1..279
                     /locus_tag="JNY02_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           65 a           79 c           78 g           57 t
ORIGIN      
        1 actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc
       61 catgaatggc gttggatgcc gggcaaccgc ccgcattatg ggcgttggcc tcaacacgat
      121 tttccgccat ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt
      181 gacgtcatcg tctgcgcgga aatggacgaa cagtggggat acgtcggggc taaatcgcgc
      241 cagcgctggc tgttttacgc gtatgacagg ctccggaag
//
LOCUS       DADWTH010000375          279 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.383, whole genome shotgun sequence.
ACCESSION   DADWTH010000375 DADWTH010000000
VERSION     DADWTH010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.383"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            96..>279
                     /locus_tag="JNY02_004455"
     CDS             96..>279
                     /locus_tag="JNY02_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435659.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQIL"
BASE COUNT           76 a           71 c           65 g           67 t
ORIGIN      
        1 tcaggtcagg cacttttgcc gggaccgtct gtaacgtgga tgccggtacc tgctccccgt
       61 ggttatctgg ttaacccata tacaagggag acagaatgac cgaatccagc gattacgaat
      121 ccgtccaggt ctttatcggc gttgatgtcg gtaaagatac gcatcacgct gttgccatta
      181 atcgttcagg taaacgcctg ttcgataaag cattacccaa cgacgaaaac aaactcaggt
      241 cgctaatatc tgacctgaaa caacatggtc agatactgc
//
LOCUS       DADWTH010000376          278 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.384, whole genome shotgun sequence.
ACCESSION   DADWTH010000376 DADWTH010000000
VERSION     DADWTH010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.384"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            24..100
                     /locus_tag="JNY02_004456"
     tRNA            24..100
                     /locus_tag="JNY02_004456"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="JNY02_004457"
     tRNA            143..218
                     /locus_tag="JNY02_004457"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DADWTH010000377          277 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.385, whole genome shotgun sequence.
ACCESSION   DADWTH010000377 DADWTH010000000
VERSION     DADWTH010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.385"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>277
                     /locus_tag="JNY02_004458"
     CDS             <1..>277
                     /locus_tag="JNY02_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBB9435660.1"
                     /translation="KGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLV
                     KLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           80 a           67 c           85 g           45 t
ORIGIN      
        1 taaaggttat tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca
       61 ctggatgata cctctcagaa agggagcgca atatgaagag ctcagaaaac tgggtaaagg
      121 cgatcatctg gtgaagctga aaaccagccc gcaggcacga aaaaagtggc cgggactggg
      181 aaatgaagtg acagcccgcc tgctgaccgt gacgcgcaaa ggaaaagtct gccatctgct
      241 gacgtcgatg acggacgcca tgcgcttccc cggagga
//
LOCUS       DADWTH010000378          275 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.386, whole genome shotgun sequence.
ACCESSION   DADWTH010000378 DADWTH010000000
VERSION     DADWTH010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.386"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>275
                     /locus_tag="JNY02_004459"
     CDS             <1..>275
                     /locus_tag="JNY02_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435661.1"
                     /translation="YVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRK
                     APPGIDMNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKK"
BASE COUNT           77 a           71 c           69 g           58 t
ORIGIN      
        1 tttacgtcac ccataatccg aaaaaaacag aacaaaaagc cggagtcagt cagtatccat
       61 taacacccgg ttcgctttat gttggcgtgt acggcggcag cccggcagat atcgcggcgg
      121 ccatctggcg taaggctccg ccgggtattg atatgaatgg caacacaacg ttcactgttg
      181 cagataagga gtacgatccg ccgtatcctg aatacgtgat cagctggcag acactcaaac
      241 ccgtcagtct gcatgtcagt gtgacgctga aaaaa
//
LOCUS       DADWTH010000379          273 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.387, whole genome shotgun sequence.
ACCESSION   DADWTH010000379 DADWTH010000000
VERSION     DADWTH010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.387"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>273)
                     /locus_tag="JNY02_004460"
     CDS             complement(<1..>273)
                     /locus_tag="JNY02_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435662.1"
                     /translation="HIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRN
                     MQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRK"
BASE COUNT           61 a           85 c           72 g           55 t
ORIGIN      
        1 cttttccggg gacggatctc aaggattgtg gtgtccggaa aagcctggcg atcccataaa
       61 gaaccgtcac tcagatgcgt cacaatcacc atattgcatc cggcatcgac cagaggcgtc
      121 acaggggtat ttccctgcat atttcgccat cctcccatac caccatcacc gaacattgtc
      181 ccctgaacct cacggggacg gaacagcagg ggcagcgcgg ctgacgcaag cagagcctct
      241 ttctgctgtc cgcggggcag gctctggata tgc
//
LOCUS       DADWTH010000380          272 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.388, whole genome shotgun sequence.
ACCESSION   DADWTH010000380 DADWTH010000000
VERSION     DADWTH010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.388"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT           76 a           55 c           60 g           81 t
ORIGIN      
        1 gcatcgggtt ggacaattta caaaagaaag ataacccata ttgtataatg cgtttaaata
       61 tgccgtctta tataaaatga tggtcagatt aatatgcgga ttatctttct acgcaaggag
      121 tatttatctc tactcccgtc aatgattgca tctcttttct ctgctaacgg tgtcgcggcg
      181 gccattgatt tatgccaggg atatgatatc aaagcgagtt gtcacgccag caggcaaagc
      241 ctttcaggca ttacgcagga ctggagtatc gc
//
LOCUS       DADWTH010000381          270 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.389, whole genome shotgun sequence.
ACCESSION   DADWTH010000381 DADWTH010000000
VERSION     DADWTH010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.389"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>270)
                     /locus_tag="JNY02_004461"
     CDS             complement(<1..>270)
                     /locus_tag="JNY02_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBB9435663.1"
                     /translation="EGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNA
                     FVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSATISAPEH"
BASE COUNT           76 a           50 c           62 g           82 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattt gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc
//
LOCUS       DADWTH010000382          268 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.390, whole genome shotgun sequence.
ACCESSION   DADWTH010000382 DADWTH010000000
VERSION     DADWTH010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.390"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(157..>268)
                     /locus_tag="JNY02_004462"
     CDS             complement(157..>268)
                     /locus_tag="JNY02_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB9435664.1"
                     /translation="SVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT           75 a           53 c           52 g           88 t
ORIGIN      
        1 ggcttgatga ttcgagcatt ttctgcccgc agtgccgata attcatcgct gtcagatttg
       61 tgaaccatgc cctgctgtaa atctccgcat agattatgtc tgtttgatct cagtatcgta
      121 aaaaagcggc tctgcaagtc ctcctaattg gtgacattac cgaccatttt tcttccttat
      181 gttttgaggg tatgtcttta aatatttaaa gaaaacttta gaaaaatacg atggtgaact
      241 gtaacctaac tcagatgata tgacagaa
//
LOCUS       DADWTH010000383          267 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.391, whole genome shotgun sequence.
ACCESSION   DADWTH010000383 DADWTH010000000
VERSION     DADWTH010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.391"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..124
                     /locus_tag="JNY02_004463"
     CDS             <1..124
                     /locus_tag="JNY02_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBB9435665.1"
                     /translation="GDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            121..>267
                     /locus_tag="JNY02_004464"
     CDS             121..>267
                     /locus_tag="JNY02_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBB9435666.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANA"
BASE COUNT           70 a           62 c           78 g           57 t
ORIGIN      
        1 gggcgatatc aaaggcgcat gtgaccagct acgtcgctgg acatatgctg gcggtaagca
       61 atggaaaggg ctgatgaccc gtcgtgagat tgagcgtgaa gtctgtttgt gggggcagca
      121 atgagcagag tcaccgcgat tatctccgct ctggttatct gcatcatcgt ctgcctgtca
      181 tgggctgtta atcattaccg tgataacgcc attacctaca aagagcagcg cgacaaaaac
      241 gcaagagaac tgaagctggc gaacgca
//
LOCUS       DADWTH010000384          264 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.392, whole genome shotgun sequence.
ACCESSION   DADWTH010000384 DADWTH010000000
VERSION     DADWTH010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.392"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>264)
                     /locus_tag="JNY02_004465"
     rRNA            complement(<1..>264)
                     /locus_tag="JNY02_004465"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           53 a           90 c           54 g           67 t
ORIGIN      
        1 ttttccggat ttgcctggaa aaccagccta cacgcttaaa ccgggacaac cgtcgcccgg
       61 ccaacatagc cttctccgtc cccccttcgc agtaacacca agtacaggaa tattaacctg
      121 tttcccatcg actacgcctt tcggcctcgc cttaggggtc gactcaccct gccccgatta
      181 acgttggaca ggaacccttg gtcttccggc gagcgggctt ttcacccgct ttatcgttac
      241 ttatgtcagc attcgcactt ctga
//
LOCUS       DADWTH010000385          256 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.393, whole genome shotgun sequence.
ACCESSION   DADWTH010000385 DADWTH010000000
VERSION     DADWTH010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.393"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>256)
                     /locus_tag="JNY02_004466"
     CDS             complement(<1..>256)
                     /locus_tag="JNY02_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB9435667.1"
                     /translation="LKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREE
                     ARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT           61 a           67 c           48 g           80 t
ORIGIN      
        1 agacacttcc gagccgttga taatactggt tttcatattc tgtcggtgac atctgttcgc
       61 tagaaccatg ccgacgctta ctgttataaa acatttcgat gtaatcaaaa atatcactgc
      121 gggcttcttc ccgcgttccg tagatctttt tctttatccg ttcacgtttc aacaactgga
      181 aaaaactttc tgcaaccgca ttatcatggc agttaccgcg acggctcatg ctaccctcca
      241 ggccgtgtga tttcag
//
LOCUS       DADWTH010000386          256 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.394, whole genome shotgun sequence.
ACCESSION   DADWTH010000386 DADWTH010000000
VERSION     DADWTH010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.394"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>256
                     /locus_tag="JNY02_004467"
     CDS             <1..>256
                     /locus_tag="JNY02_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435668.1"
                     /translation="YRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFN
                     NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQ"
BASE COUNT           76 a           57 c           61 g           62 t
ORIGIN      
        1 attaccgggt catacaggag cagtttggtc tgaaaacatc aatgtcgcgt aaaggtaact
       61 gttacgacaa cgctccgatg gaaagcttct ggggaacgct gaaaaatgag agcctgagcc
      121 actatcgttt taataaccgg gatgaagcca tctcagtaat acgggaatac attgagattt
      181 tctacaatcg tcagcgtcgt cactctcgtc tggggaatat ctccccggca gccttcaggg
      241 aaaaatatca tcagat
//
LOCUS       DADWTH010000387          252 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.395, whole genome shotgun sequence.
ACCESSION   DADWTH010000387 DADWTH010000000
VERSION     DADWTH010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.395"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>252
                     /locus_tag="JNY02_004468"
     CDS             <1..>252
                     /locus_tag="JNY02_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB9435669.1"
                     /translation="TPRAGRPWAEHRKIINGMFWVLCSGAPWRDLPERYGAWKTVYNR
                     FNRWSKSGVINIIFNRLLSLLDANGFIDWSATALDGSNIR"
BASE COUNT           60 a           59 c           66 g           67 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccggtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gg
//
LOCUS       DADWTH010000388          251 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.396, whole genome shotgun sequence.
ACCESSION   DADWTH010000388 DADWTH010000000
VERSION     DADWTH010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.396"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>251
                     /gene="tnpB"
                     /locus_tag="JNY02_004469"
     CDS             <1..>251
                     /gene="tnpB"
                     /locus_tag="JNY02_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBB9435670.1"
                     /translation="IWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIFVFRGRSGKM
                     VKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQ"
BASE COUNT           52 a           66 c           79 g           54 t
ORIGIN      
        1 aatctggatc atcgctggca tcacagatat gcgttgtggc ttcaatggcc tggcttcgaa
       61 ggtgcagaac acgctgaaag atgacccgtt ctccgggcat atcttcgtct tccggggccg
      121 cagtggcaaa atggtgaaaa tactgtgggc cgatcgtgac gggttatgcc tgttcgccaa
      181 acgcctggaa cggggccgct tcgtctggcc ggtgacccgg gaagggaaag tgcacctgac
      241 gccagctcag t
//
LOCUS       DADWTH010000389          248 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.397, whole genome shotgun sequence.
ACCESSION   DADWTH010000389 DADWTH010000000
VERSION     DADWTH010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.397"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>248
                     /locus_tag="JNY02_004470"
     CDS             <1..>248
                     /locus_tag="JNY02_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBB9435671.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT           52 a           68 c           69 g           59 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgc
//
LOCUS       DADWTH010000390          242 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.398, whole genome shotgun sequence.
ACCESSION   DADWTH010000390 DADWTH010000000
VERSION     DADWTH010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.398"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>242
                     /locus_tag="JNY02_004471"
     CDS             <1..>242
                     /locus_tag="JNY02_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBB9435672.1"
                     /translation="ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY"
BASE COUNT           56 a           57 c           77 g           52 t
ORIGIN      
        1 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
       61 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
      121 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
      181 gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt
      241 at
//
LOCUS       DADWTH010000391          235 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.denovo.399, whole genome shotgun sequence.
ACCESSION   DADWTH010000391 DADWTH010000000
VERSION     DADWTH010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.denovo.399"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>235
                     /locus_tag="JNY02_004472"
     CDS             <1..>235
                     /locus_tag="JNY02_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBB9435673.1"
                     /translation="GLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHI
                     PRMLSTDNFKKIKLRDISLEDAIKASNYEEINNK"
BASE COUNT           92 a           28 c           43 g           72 t
ORIGIN      
        1 gggttgcatc ttattgattt tataagaaag agtaaagatc aaggattccg agagttttgt
       61 tataacaaaa acattgatcc tgttagtctg gatagaatta taaactttgt gtttcagctt
      121 gagtatcata taccaagaat gctaagtaca gataacttca aaaaaataaa actcagagac
      181 atatctttgg aggatgcgat taaagcatct aattatgaag agattaacaa taaag
//
LOCUS       DADWTH010000392        17423 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.guided.078, whole genome shotgun sequence.
ACCESSION   DADWTH010000392 DADWTH010000000
VERSION     DADWTH010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.guided.078"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="JNY02_004473"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="JNY02_004473"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBB9435674.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="JNY02_004474"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="JNY02_004474"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBB9435675.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="JNY02_004475"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="JNY02_004475"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBB9435676.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            3030..6341
                     /gene="mscM"
                     /locus_tag="JNY02_004476"
     CDS             3030..6341
                     /gene="mscM"
                     /locus_tag="JNY02_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBB9435677.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNY02_004477"
     CDS             complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNY02_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBB9435678.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNY02_004478"
     CDS             complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNY02_004478"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBB9435679.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9515..11323
                     /gene="frdA"
                     /locus_tag="JNY02_004479"
     CDS             9515..11323
                     /gene="frdA"
                     /locus_tag="JNY02_004479"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBB9435680.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            11316..12050
                     /gene="frdB"
                     /locus_tag="JNY02_004480"
     CDS             11316..12050
                     /gene="frdB"
                     /locus_tag="JNY02_004480"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBB9435681.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12061..12456
                     /gene="frdC"
                     /locus_tag="JNY02_004481"
     CDS             12061..12456
                     /gene="frdC"
                     /locus_tag="JNY02_004481"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBB9435682.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12467..12826
                     /gene="frdD"
                     /locus_tag="JNY02_004482"
     CDS             12467..12826
                     /gene="frdD"
                     /locus_tag="JNY02_004482"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBB9435683.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12889..14022
                     /locus_tag="JNY02_004483"
     CDS             12889..14022
                     /locus_tag="JNY02_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBB9435684.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            14111..14644
                     /gene="blc"
                     /locus_tag="JNY02_004484"
     CDS             14111..14644
                     /gene="blc"
                     /locus_tag="JNY02_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBB9435685.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14641..14870)
                     /locus_tag="JNY02_004485"
                     /pseudo
     CDS             complement(14641..14870)
                     /locus_tag="JNY02_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="JNY02_004486"
     CDS             complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="JNY02_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBB9435686.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="JNY02_004487"
     CDS             complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="JNY02_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBB9435687.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(15480..16046)
                     /gene="efp"
                     /locus_tag="JNY02_004488"
     CDS             complement(15480..16046)
                     /gene="efp"
                     /locus_tag="JNY02_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBB9435688.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16088..17116
                     /gene="epmB"
                     /locus_tag="JNY02_004489"
     CDS             16088..17116
                     /gene="epmB"
                     /locus_tag="JNY02_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBB9435689.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4338 a         4568 c         4487 g         4030 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcgat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 agacttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacgcgtccg gatttctacg acggcgtgaa acccatagcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat cgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaggccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggg cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggagc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatttact taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggctgatt atggcgctga
     4861 tgatgttcga taacctcgac gaccgcgaat tttccggttc gctgggacgg ctttgcttta
     4921 tcctcatttg cggtgcgctg gcggtggtca ccctaagcct gaaaaaagcc ggtattccgt
     4981 tgtatctcaa caaagaaggc agcggcgaca acattaccaa ccatatgctg tggaacatga
     5041 tgattggcgc accactggtt gccattctgg cgtcggcggt gggttatctg gcaacggcgc
     5101 aggcactgtt agcgaggctt gaaacctcgg ttgccatctg gttcctgcta ctggtggttt
     5161 atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt gaccgggcga
     5221 agcatcgccg ggcagagatg ttagcgcaac gtgcgcgtgg cgaagaggaa gcgcatcatc
     5281 acagtagccc ggaaggagca attgaagtcg atgaaagcga agtcgatctc gatgccatca
     5341 gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg cgttcggtca
     5401 ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt tcgctgtggg
     5461 atgtcacctc cacggtacag ggcgtagaaa gtctggagcc aattaccctc ggtgcggtgc
     5521 tgattgccat tctggtattt atcatcacca cgcagctggt gcgcaacctg cccgcgctgc
     5581 tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac gccatcacca
     5641 ccatcacaaa atatctgctg atgctgattg gcgggctggt cggcttctca atgattggta
     5701 ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc ggttttggtt
     5761 tgcaggaaat tttcgccaac tttatctctg gcctgatcat cctgttcgaa aaaccgattc
     5821 gcattggcga tacggtaacg atccgcgatc ttactggtag cgtgacaaaa attaacaccc
     5881 gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac aaggcgttta
     5941 ttaccgcgca gtttatcaac tggtcgctct ctgactcggt cacgcgcgta gtgttgacga
     6001 ttccggcccc tgccgatgcc aacagtgaag aagtgacgga aatcctgctc accgcagcgc
     6061 gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg gatctgcaac
     6121 aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac cgtatgccgc
     6181 tacgccatga gatccaccag ctgattctgg caggcttcca cgcccacggt atcgatatgc
     6241 cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg gggagaacgc
     6301 tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta atgcatgaag ctgccaaatg
     6361 atgtcaacgt ggctcattta gcagcttaac atcatcttca gttatcaccg gccctggaga
     6421 gtatcatcca tacgcattac ttcctgacaa ctcatcgccg gagagacgat gagttgcagg
     6481 atttcatatg atcagtcgag agctaactca ggtttattcg ccatgcgact gcaatagttc
     6541 tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg
     6601 ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc
     6661 accaccagaa cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt gaaaatcaca
     6721 aatggtctgt cgagatcctg acgtgcttta aaaaacggga aagccagcgc gaggaacagg
     6781 taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg
     6841 gtaccgccac caaacgaaac cagcagaatg aagacagtaa ccaacccgca ctgcatccac
     6901 atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg ccacaacgct
     6961 ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg
     7021 gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat
     7081 gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc
     7141 acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg
     7201 ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa
     7261 acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc
     7321 gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg
     7381 gactgataac ccgggttcgg tgatgcaagg aaattaatat cctgcgcgaa atgcccacca
     7441 tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca
     7501 ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg
     7561 accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca
     7621 atacgccagt gctgggtcat gtcactacca tagaggaatg ttgagaacgg tacccaaact
     7681 ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg
     7741 aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc ttcttctttg
     7801 cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca
     7861 gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg
     7921 gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt
     7981 atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa
     8041 ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga taccttgatg
     8101 atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca
     8161 gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc
     8221 ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca
     8281 gacgatcaac acctaatgcc acgccggagc agtcaggcat accgactttc aaggcttcaa
     8341 tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt
     8401 gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct
     8461 caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt
     8521 gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttaccaa
     8581 tatttgcctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg
     8641 tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg
     8701 cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg
     8761 ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca
     8821 tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca
     8881 tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca gcaaccagca
     8941 ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc
     9001 cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc
     9061 tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg
     9121 ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg
     9181 tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga
     9241 cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg
     9301 atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac
     9361 taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat
     9421 aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg
     9481 gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc
     9541 cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc
     9601 aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc
     9661 agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga
     9721 tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca
     9781 ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc gtcgcccgga
     9841 tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc
     9901 cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca
     9961 gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg
    10021 cggcctggta gcaatgaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt
    10081 cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt
    10141 taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt
    10201 cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg
    10261 tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat
    10321 gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga
    10381 caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacactatct ccacgccgcg
    10441 tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct
    10501 gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat
    10561 tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga
    10621 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
    10681 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
    10741 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
    10801 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
    10861 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
    10921 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
    10981 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
    11041 gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
    11101 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct
    11161 caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt
    11221 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
    11281 taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
    11341 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
    11401 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
    11461 caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga
    11521 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacagta
    11581 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
    11641 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
    11701 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
    11761 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
    11821 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
    11881 agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg
    11941 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
    12001 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
    12061 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
    12121 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
    12181 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
    12241 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct
    12301 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
    12361 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
    12421 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
    12481 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
    12541 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
    12601 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
    12661 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
    12721 cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc
    12781 tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt
    12841 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
    12901 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
    12961 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
    13021 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
    13081 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
    13141 tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc
    13201 gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
    13261 cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca
    13321 agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt
    13381 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
    13441 agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg
    13501 attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca
    13561 gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa
    13621 gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt
    13681 caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc
    13741 ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac
    13801 aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta
    13861 cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt
    13921 attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagag
    13981 agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa
    14041 ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa
    14101 ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt
    14161 tgcctgtagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgccaa
    14221 acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact
    14281 ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa
    14341 aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg
    14401 cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa
    14461 cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta
    14521 cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc
    14581 agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag
    14641 ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag
    14701 acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc
    14761 gccaataccc gtccacacgg catcaaacgg ctaaagccgt gggtatattt caggccaacg
    14821 gcccatacca cttccggcag accagcaata actaagataa tccaggacat atcaggctcc
    14881 ggaaatttgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat
    14941 ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt tatgacgatc
    15001 gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt gctgcgcttt
    15061 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
    15121 ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
    15181 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
    15241 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga agagaagaaa
    15301 aattagctgg cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg
    15361 caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt
    15421 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
    15481 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt
    15541 tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagccgg tttgccgcca
    15601 gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag
    15661 ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga
    15721 tccagcagcc atttagcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa
    15781 gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata
    15841 tcgacaacat cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta
    15901 ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg
    15961 aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga
    16021 aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag
    16081 ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca
    16141 acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga
    16201 cgaaaaactg ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc
    16261 atttatcgat cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac
    16321 ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc gacccactgg aagaacagca
    16381 cagcgtagtg cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg
    16441 cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca
    16501 gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga
    16561 cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt
    16621 gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc
    16681 gattgtgatc ccggcacgta tcaccgaggc gctggttgaa cgctttgccc gttctacgct
    16741 gcaaatcttg ctggtgaatc acatcaatca tgccaatgag gtagatgaaa cgttccgtca
    16801 ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg ttctgttacg
    16861 tggtgtgaac gataacgcac aaacgttggc aaacctgagt aatgcgttgt tcgatgccgg
    16921 cgtgatgccg tattacctgc atgtgctaga taaagtacag ggcgcggcgc attttatggt
    16981 gagtgatgac gaagcacggc agattatgcg tgagttgctg acattggtgt cgggatatct
    17041 ggtgccgaaa ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc tggatctcca
    17101 gctacgccag cagtaactga ttaaacaggg aagttaaaca ttaaaattgc ctgatgcgct
    17161 acgcttatca ggcctacatc gtgcattgaa attgaatgga tttgcaatga actgtaggcc
    17221 ggataaggcg tttacgccgc atccggcaag agcaacaaac gcaagtacag caacctgtca
    17281 ttaaactgag aaatttaatc tttgcacgtt gcataacctt taattattca tatgcattgc
    17341 tgttgttact ttttatatag ctaacaccaa taattaacct ttcttttcgg taatgactcc
    17401 aacttactga tagtgtttta tgt
//
LOCUS       DADWTH010000393        10830 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.guided.122, whole genome shotgun sequence.
ACCESSION   DADWTH010000393 DADWTH010000000
VERSION     DADWTH010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.guided.122"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            59..1103
                     /locus_tag="JNY02_004490"
                     /pseudo
     CDS             59..1103
                     /locus_tag="JNY02_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1165..1779
                     /locus_tag="JNY02_004491"
                     /pseudo
     CDS             1165..1779
                     /locus_tag="JNY02_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="JNY02_004492"
     CDS             complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="JNY02_004492"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBB9435690.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3555..4013
                     /gene="gpt"
                     /locus_tag="JNY02_004493"
     CDS             3555..4013
                     /gene="gpt"
                     /locus_tag="JNY02_004493"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBB9435691.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4105..5349
                     /gene="frsA"
                     /locus_tag="JNY02_004494"
     CDS             4105..5349
                     /gene="frsA"
                     /locus_tag="JNY02_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBB9435692.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5407..5808
                     /gene="crl"
                     /locus_tag="JNY02_004495"
     CDS             5407..5808
                     /gene="crl"
                     /locus_tag="JNY02_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBB9435693.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5847..6902)
                     /gene="phoE"
                     /locus_tag="JNY02_004496"
     CDS             complement(5847..6902)
                     /gene="phoE"
                     /locus_tag="JNY02_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBB9435694.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7190..8293
                     /gene="proB"
                     /locus_tag="JNY02_004497"
     CDS             7190..8293
                     /gene="proB"
                     /locus_tag="JNY02_004497"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBB9435695.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8305..9558
                     /gene="proA"
                     /locus_tag="JNY02_004498"
     CDS             8305..9558
                     /gene="proA"
                     /locus_tag="JNY02_004498"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBB9435696.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9673..9748
                     /locus_tag="JNY02_004499"
     tRNA            9673..9748
                     /locus_tag="JNY02_004499"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9706..9708,aa:Thr,seq:cgt)
     gene            complement(9763..>10830)
                     /locus_tag="JNY02_004500"
     CDS             complement(9763..>10830)
                     /locus_tag="JNY02_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB9435697.1"
                     /translation="TLTRWPVFVDHFKAWRVKNLGESPKKLFKEACIRWLREKSDKKS
                     IDDDKSIISFWMLHFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPV
                     PEYKPKLASKGTKTRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESK
                     RLFAEIAPHFFPVVMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVP
                     LNETACQILRKQQGLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGI
                     SNFRFHDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDA
                     IISRNGTNTAQEENVVYLNAR"
BASE COUNT         2582 a         2705 c         2828 g         2715 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggtcc atagcggctc gagggagcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca ggcccgtcag gagtgaggaa acattgcatt
      901 cgctggcgca tggggctggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag
      961 cgaaatacac agcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg
     1021 ataaacaact catcttcgaa gaagcgccac aagcctataa atcggctgaa agtgtggtgc
     1081 aatgtctggg caggccggat taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct
     1141 caaaaacagt ggagggaaaa aaggatgatc ttgctataac tctcctcagc gcaggggccg
     1201 gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga agctgcccga
     1261 caagacgtcg cggtaacggt gctggaaaca gaaacaggcc gctactctga cacgctgcgt
     1321 tcggcgctga tttctctgga tggcgacaac gcatgggcgt taagcgaaag ctggtgtggc
     1381 actattcagt agatttgccc gagcccgtat cgcccacatc acgggcgtaa aaactggttt
     1441 ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat ccggtatgag
     1501 acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc
     1561 gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag ccagcatgct
     1621 aacaaacggc tggcgcgatt gctgattgcc tggaagctgg atcagcagca ataggaaaat
     1681 agcgcgtagc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg tggcaacccg
     1741 cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa
     1801 ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc
     1861 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
     1921 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
     1981 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
     2041 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
     2101 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
     2161 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
     2221 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac cacacctttt
     2281 gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
     2341 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
     2401 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
     2461 gatttcagtg cgtcgacttt atcagctgtg acagcaatgg tcgcaaaggc ttcacgcggg
     2521 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
     2581 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
     2641 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
     2701 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
     2761 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
     2821 ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt cagcagcact
     2881 tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     2941 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     3001 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     3061 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     3121 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     3181 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat agaacagatt
     3241 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     3301 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     3361 caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc gcgtcaggga
     3421 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagata
     3481 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     3541 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     3601 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     3661 gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3721 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3781 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     3841 ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     3901 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg
     3961 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     4021 aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca
     4081 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     4141 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggtgcacaa
     4201 ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac
     4261 cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt
     4321 attgtgatga gcgatgctga acgtaccgac gatgatttat acgataccgt gattggctac
     4381 cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc
     4441 tgtacggaag aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac
     4501 aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta
     4561 tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag
     4621 tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc
     4681 ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc
     4741 ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccgtcg
     4801 gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc
     4861 ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc
     4921 cgtttcggcg ctaacgttgc cgtgcgtttg gcataccttg aatcgccgcg tctgaaagcg
     4981 gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag
     5041 gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgac
     5101 gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctggggcgt
     5161 cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag
     5221 gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac
     5281 ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc
     5341 ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag
     5401 atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca
     5461 ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg
     5521 gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg ctggtggatg
     5581 gagctggaag cgcaggaatc ccgttttacc tacagctacc agtttggtct gttcgataaa
     5641 gcaggcgact ggaagagtgt tccggtaaaa gatactgaag tggttgaacg actggagcac
     5701 accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg
     5761 gaaccggcgg atgattttcg tgatgaaccg gtgaagttaa cggcgtgagt gaaatgtgcc
     5821 ggatgcatca catccggcaa tattcattaa aactgatacg tcatcccaac cgcgacagta
     5881 tcatcattat taatattcag cttattatcg ctatccagtt tgttgatttt ataatcaaca
     5941 aacgctgaca tatttttgtt gaaataatac gtagcaccga cgtcgatata attgaccaga
     6001 tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc cagcgatgga
     6061 cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgtgt cttattggca
     6121 aagccgccag ttattggcgt cattttgcgt gtttcagaat agaaagttgc cagataaata
     6181 ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc tgtgccacgg
     6241 ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat aggccccact aatggcgaaa
     6301 tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc catcgccgtt ttgcttttta
     6361 acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc atcgataacg
     6421 ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt cataaagttg
     6481 tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca tatcggtcca ggcttccacg
     6541 tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt atatttcaac
     6601 ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt atttccggca
     6661 aactccgctt cccaccgacc ataaccagtc agttgatcgt taatttgagt ttcaccttta
     6721 aaaccaaaac ggatataact ctggtcgcca tctttactgg cgttatcact catataatgc
     6781 atggctttaa ctttgccata gacatccagt ttattaccgt ctttattata tatttctgca
     6841 gcctgtacag atgcagatgc cacaatgccc attaccacta atgccagagt gctctttttc
     6901 attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc cgttgaaaac
     6961 aggaaagttt ttaacctgag attgttaaag atatattaca gattaatgat attctgaaaa
     7021 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg cttgctgatc
     7081 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
     7141 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
     7201 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
     7261 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
     7321 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
     7381 actgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga gtcgactgat
     7441 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
     7501 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
     7561 gctcgatcac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
     7621 taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
     7681 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca gcaatccgca
     7741 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
     7801 cagcgtttca ggcctcggaa ctggcggcat gagtactaaa ttgcaggccg ctgacgtggc
     7861 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
     7921 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
     7981 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     8041 aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     8101 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     8161 ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca
     8221 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     8281 gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     8341 tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc
     8401 gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac
     8461 gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg
     8521 ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc ggtggggcag
     8581 gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg
     8641 ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg
     8701 tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg tcgcactaac
     8761 gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc ggcgggtgcc
     8821 gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa
     8881 tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag
     8941 tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagc
     9001 gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca
     9061 tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca
     9121 ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcgctgaca
     9181 cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta tgacgatgag
     9241 tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat
     9301 attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc
     9361 cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt
     9421 accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac
     9481 gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat
     9541 tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg
     9601 ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca
     9661 tgtcctttca gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc
     9721 gtaggttcga ctcctattat cggcaccatt taaatcaata agttacctcg catttaagta
     9781 aaccacgttc tcctcttgtg ccgtatttgt gccattgcga cttataatcg catcgatttt
     9841 gcttgcgtgc tcggtgagat gcccggctga aaggtgggcg tatctttgaa ccatttcgag
     9901 agtttcccat cctcccatct ctttaagtgc aagaagagag acaccggact gaaccagcca
     9961 gcttgcccag gtatgcctca gatcatggaa gcggaagttg ctaatgcctg cccgctttaa
    10021 cgctcccttc catgccttgt tgctgtcggt tctcatcttc cttaccgctg ctgtttttgt
    10081 tccgtcgctt cggtaggcag gtttggtgtg gacaaatacc catctcttat ggagcccctg
    10141 ctgttttctt aatatctggc atgcggtttc gttaagagga actccgatcg cattgccagc
    10201 ttttgtttca tcagggtgca tccatgccat tttcttatcc agatcgacct gtgaccactc
    10261 aaggtctgta acgttggaac ggcgaaggcc tgtcgtgatt gcaaacatga ccacagggaa
    10321 gaagtgagga gcaatttctg caaacaggcg cttcgattcc tcctctgtaa gccatctgat
    10381 gcgtccattc ttaacgcgtg gtgttgatat tttgggtgcc ctgtcaagcc atccccattc
    10441 aacagccata ttgagaatgg cgcgaagtat tgccagatgc ctcgtcttcg ttcctttgct
    10501 tgcaagcttt ggtttatact ccggcaccgg attgccaagt cgcaaacacc tgtcccggct
    10561 catctcccag ttcaggcgat ggcggcggtt ttccatcccg tctaccgcct ccattatttt
    10621 ttctgttgtt atgtcagaga gaatggcttc tctgaagtgc aacatccaga acgatataat
    10681 gctcttgtca tcatcaatgg acttcttatc cgatttctca cgcagccacc gtatgcaggc
    10741 ttccttgaat agctttttcg gtgattcccc gagatttttt actctccacg ctttgaagtg
    10801 gtcaacaaaa actggccacc gagttagagt
//
LOCUS       DADWTH010000394         8853 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.guided.141, whole genome shotgun sequence.
ACCESSION   DADWTH010000394 DADWTH010000000
VERSION     DADWTH010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.guided.141"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            264..923
                     /locus_tag="JNY02_004501"
     CDS             264..923
                     /locus_tag="JNY02_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBB9435698.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="JNY02_004502"
     CDS             complement(1124..1501)
                     /locus_tag="JNY02_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB9435699.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="JNY02_004503"
     CDS             complement(1568..4534)
                     /locus_tag="JNY02_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBB9435700.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5097)
                     /locus_tag="JNY02_004504"
     CDS             complement(4537..5097)
                     /locus_tag="JNY02_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB9435701.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5223..5573)
                     /locus_tag="JNY02_004505"
     CDS             complement(5223..5573)
                     /locus_tag="JNY02_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBB9435702.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="JNY02_004506"
     CDS             complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="JNY02_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBB9435703.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6999..7829
                     /locus_tag="JNY02_004507"
     CDS             6999..7829
                     /locus_tag="JNY02_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBB9435704.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7942..8733
                     /gene="aadA1"
                     /locus_tag="JNY02_004508"
     CDS             7942..8733
                     /gene="aadA1"
                     /locus_tag="JNY02_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBB9435705.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         2016 a         2367 c         2497 g         1973 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5101 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5161 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5221 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5281 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5341 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5401 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5461 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5521 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5641 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5701 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5761 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5821 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5881 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5941 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     6001 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6061 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6121 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6181 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6241 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6301 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6361 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6421 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6481 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6541 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6601 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6841 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6901 gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat taattgttag
     6961 ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca atacatatca
     7021 acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag
     7081 atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg
     7141 atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg
     7201 caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag
     7261 atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca
     7321 atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc
     7381 aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag
     7441 acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct
     7501 taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc
     7561 cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata
     7621 atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa
     7681 acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg
     7741 cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga
     7801 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
     7861 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
     7921 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
     7981 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8041 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8101 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8161 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8221 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8281 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8341 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8401 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8461 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8521 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8581 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8641 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     8701 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     8761 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgcggtgatg
     8821 ctgccaactt actgatttag tgtatgatgg tgt
//
LOCUS       DADWTH010000395         6385 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.guided.171, whole genome shotgun sequence.
ACCESSION   DADWTH010000395 DADWTH010000000
VERSION     DADWTH010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.guided.171"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="JNY02_004509"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="JNY02_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBB9435706.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1451..1978
                     /locus_tag="JNY02_004510"
                     /pseudo
     CDS             1451..1978
                     /locus_tag="JNY02_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            1999..2472
                     /locus_tag="JNY02_004511"
     CDS             1999..2472
                     /locus_tag="JNY02_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBB9435707.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2547..2822
                     /locus_tag="JNY02_004512"
                     /pseudo
     CDS             2547..2822
                     /locus_tag="JNY02_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2822..3658
                     /locus_tag="JNY02_004513"
     CDS             2822..3658
                     /locus_tag="JNY02_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBB9435708.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3765..4169)
                     /locus_tag="JNY02_004514"
                     /pseudo
     CDS             complement(3765..4169)
                     /locus_tag="JNY02_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4213..4602)
                     /locus_tag="JNY02_004515"
     CDS             complement(4213..4602)
                     /locus_tag="JNY02_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435709.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4820..5260
                     /locus_tag="JNY02_004516"
     CDS             4820..5260
                     /locus_tag="JNY02_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBB9435710.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(6005..6154)
                     /locus_tag="JNY02_004517"
     CDS             complement(6005..6154)
                     /locus_tag="JNY02_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB9435711.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1588 a         1650 c         1652 g         1495 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cgcttcatca gaaaactgaa
     1441 ggaacctcca ttgaatcgaa ctaatatttt ttttggtgaa tcgcattctg actggttgcc
     1501 tgtcagaggc ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc
     1561 gaaaatcgca cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg
     1621 gctcaaaggt cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg
     1681 tgcatgcttg gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga
     1741 tttgctcaaa gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt
     1801 tgatcaatgt ccgtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt
     1861 gtcccgcaat gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct
     1921 cgatcttttg gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccgt
     1981 taacccagga tgagaacctt gaaagtatca ttgatagctg cgaaagcgaa aaacggcgtg
     2041 attggttgcg gtccagacat accctggtcc gcgaaagggg agcagctact ttttaaagca
     2101 ttgacctaca atcagtggct tctggtgggt cgcaagacgt ttgaatctat gggcgcactc
     2161 cccaatagga aatacgcggt cgttacccgc tcaggttgga catcaaatga tgacaatgta
     2221 gttgtatttc agtcaatcga agaggccatg gacaggctag ctgaattcac cggtcacgtt
     2281 atagtgtctg gtggcggaga aatttaccga gaaacattac ccatggcctc tacgctccac
     2341 ttatcgacga tcgacatcga gccagagggg gatgttttct tcccgagtat tccaaatacc
     2401 ttcgaagttg tttttgagca acactttact tcaaacatta actattgcta tcaaatttgg
     2461 aaaaagggtt aacaaagcta tgcaatcgac ggcaaaaagc ttcgttcgct tcgcgcacta
     2521 cgcctttttc cgcgattgat agcgacgata tggttgtttg ccatggtgat ccctgcatgc
     2581 cgaacttcat ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc
     2641 tcggaacagc agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact
     2701 gggcagcgcc agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg
     2761 aagcccccga ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt
     2821 gatgttcatg ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc
     2881 ggacaccttt tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt
     2941 caagggattg aaacctatag aagacattgc tgatgaactg cgcggggccg actatctggt
     3001 atggcgcaat gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct
     3061 cgaatatgcc ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc
     3121 gaccgaaatt gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc
     3181 ttctgccctt ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga
     3241 tcaaaacgca ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat
     3301 gagcaatgcc tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt
     3361 ctccagtcgc ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg
     3421 cgccgccaat atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg
     3481 cattgcacag atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct
     3541 cgaccaggcg tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga
     3601 gcaacgcgat ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat
     3661 atcaagcgac ttctcctatc ccctgggaac acatcaatct caccggagaa tatcgctggc
     3721 caaagcctta gcgtaggatt ccgccccttc ccgcaaacga ccccaaacag gaaacgcagc
     3781 tgaaacggga agctcaacac ccactgacgc atgggttgtt caggcagtac ttcatcaacc
     3841 agcaaggcgg cactttcggc catccgccgc gccccacagc tcgggcagaa accgcgacgc
     3901 ttacagctga aagcgaccag gtgctcggcg tggcaagact cgcagcgaac ccgtagaaag
     3961 ccatgctcca gccgcccgca ttggagaaat tcttcaaatt cccgttgcac atagcccggc
     4021 aattcctttc cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc
     4081 tgatagagaa gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg
     4141 ctggccgtcc tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat
     4201 acagtctatc gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag
     4261 acattgccag ccagtgcccg tcactcccac tcttacattg ttcgcagtat ctaggtacat
     4321 gttattgcgt gcaccttcat gatgtataat gttattaatg acagcagaaa gatcgacaga
     4381 attaatattc ttcgtacctg ggacagttat cgggtgtcgg ccagtgatga gaccaaagga
     4441 tgcagtataa gatgaaccac tactattatc atggccacca ttaccaccac cgttaaaact
     4501 acctcccgaa cttccagagc cgctatgacc atcattgtag gggtcatttg gtcccatgtg
     4561 accaaaacca ccgacagtac cgctatttaa ctctgtactc ataaaattat cctctttgac
     4621 ttttaaaaac agcaaaaact gtatttataa caatgtgttt atatacagta ttcgaaatac
     4681 tacgcccgtc aaaaccacac cgtcaacagg ttttttgtgt atttttcacg cctcacgggc
     4741 agcctcagac cttttgtgtt aaaacagtaa tatgttctgc tgttacaata cggtacacac
     4801 aggagacact gtcactttta tggcatggca cacagttcaa gagacactga aattaacagg
     4861 aaagtcgcgc tcgcaattat atcgtgacat ggctaaaggc cttgtcagct atcgcacagg
     4921 aaaaaatgat cgccgggagt tcgagaccag tgaactgatt cgggcctatg gcgagctgcg
     4981 cacttctgag acaccaaaaa ggcacaagga gggacactcc aaatctgtcg ttagccaggc
     5041 ggaaaatgct cagctagacg tcattcagca gaaactggac gctctgcagc agactgttac
     5101 cctaatgctt gaagacaaga tggcgcggga agcagaaaac cgcctgcgaa atgaggagag
     5161 ggaacaacta caggccgaaa ttagccgtct ggctgacgcg ctggcacagg aaaaaaagcg
     5221 gggattctgg tcgcggctgt ttcgctccgg ttcagagtaa cagtgcaggt gactctgatt
     5281 taccataaca tctgttatca gcaccgcctg agaggccctc agggcgtttc tgacgggaaa
     5341 ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg ctctgttttt
     5401 acggcatgca tgagtatgcg cacaagtgca tagcgcggaa attcacacca ttcagccggg
     5461 gtaagtcctg tttccggcgt tcttcccatc cgaaaacggt gaacggggac gcaatttatt
     5521 gcgtggccgt gaagcgttgc cggcgcagcc ggaaaacccg ctgtcagcac gttcaggaag
     5581 ggggtgatgt acaaatggca cagaggatga aagtgacgta tgagctgaag gaatgatgta
     5641 aaagtgatgt ataagtgatg taaaagtgat gtatgaactg aaggagcgat gtaattctgt
     5701 agtcaggaag aataacccgt tcatgcaggc atgaacgata cagtgaggaa cgcttttttt
     5761 gtcacgatct ccccttaacc tgcgtaaatc acccgccatg gttgattatt tttcaacctc
     5821 aaaatggtca tacacctaac gcagccgcga ttttcatcgc aactgcatga attaaaagaa
     5881 aaaaaaggaa tatttcgttt tcacgagtaa tacccgggta ttaaaaatcc actgtaatta
     5941 catgaaaata aagaaataaa aggtaatacc ggagtattac cacgtacgcg ggcaagcgaa
     6001 aaagctataa cgcttcccgg ccgacaggcg acccaaaatc cacaggggca tgcaggttct
     6061 cttcagtaac ctgcgccatt aaatcaaaaa ggtcatattc gccgggaagc tgcgtatcag
     6121 tcgaatccag ttgttcatca tattcagatg gcatggggtg atatccagta gctgagaggt
     6181 tgttagcggg tttcggggcg cagccctgaa ccagtcatgt agcgctagcg gagtgtatac
     6241 tggcttacta tgttgccacg attgcgaaaa tcagtgaagt gcttcatgtg gcaggagaaa
     6301 aaaggcagca ccggcgcgtc agcagaatat gcgatacagg agatattccg cttcctcgct
     6361 cactgactcg ctacgctcgg tcgtt
//
LOCUS       DADWTH010000396         5665 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.guided.179, whole genome shotgun sequence.
ACCESSION   DADWTH010000396 DADWTH010000000
VERSION     DADWTH010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.guided.179"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            70..285
                     /locus_tag="JNY02_004518"
     CDS             70..285
                     /locus_tag="JNY02_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBB9435712.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="JNY02_004519"
     CDS             278..664
                     /locus_tag="JNY02_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBB9435713.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="JNY02_004520"
     CDS             672..1358
                     /locus_tag="JNY02_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB9435714.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JNY02_004521"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JNY02_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBB9435715.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="JNY02_004522"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="JNY02_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBB9435716.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JNY02_004523"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JNY02_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBB9435717.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4085..4456
                     /locus_tag="JNY02_004524"
     CDS             4085..4456
                     /locus_tag="JNY02_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB9435718.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4466..>5665)
                     /locus_tag="JNY02_004525"
     CDS             complement(4466..>5665)
                     /locus_tag="JNY02_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBB9435719.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1568 a         1045 c         1230 g         1822 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc
     4261 tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa
     4321 aactatcctt gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg
     4381 atttaagtac atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt
     4441 ggaggcaatg cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     4501 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     4561 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     4621 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     4681 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     4741 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     4801 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     4861 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     4921 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     4981 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     5041 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     5101 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     5161 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     5221 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
     5281 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
     5341 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
     5401 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
     5461 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
     5521 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
     5581 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg taatgactcc
     5641 aacttactga tagtgtttta tgttc
//
LOCUS       DADWTH010000397         4471 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.guided.209, whole genome shotgun sequence.
ACCESSION   DADWTH010000397 DADWTH010000000
VERSION     DADWTH010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.guided.209"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            110..1015
                     /locus_tag="JNY02_004526"
     CDS             110..1015
                     /locus_tag="JNY02_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBB9435720.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /locus_tag="JNY02_004527"
     CDS             1012..2250
                     /locus_tag="JNY02_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB9435721.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /locus_tag="JNY02_004528"
     CDS             2250..2834
                     /locus_tag="JNY02_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB9435722.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="JNY02_004529"
     CDS             complement(3327..4091)
                     /locus_tag="JNY02_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBB9435723.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4153..4431)
                     /locus_tag="JNY02_004530"
                     /pseudo
     CDS             complement(4153..4431)
                     /locus_tag="JNY02_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
BASE COUNT          785 a         1391 c         1390 g          905 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag ccgctggaga tgccgagcgc aaaaaggatc aaggctatgg cagacggcag
     4201 cgtgaagcca gcccaagcag ccagcgcccc gctgtatcca gcccgagaca gtcctaccgc
     4261 tatgccgacc tggctgcttg caggccctgg caagaactga caaagcgcga ccaagtcagc
     4321 atagctccgt tcggagagcc agcgccgccg tgtgacaaat tcggcgcgga agtagcccaa
     4381 gtgcgcaatg gggccgccaa aagatgtcaa tccaagccgc agaaaaataa gggcactgtt
     4441 gcaaagttag cgatgaggca gccttttgtc t
//
LOCUS       DADWTH010000398         1817 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.guided.275, whole genome shotgun sequence.
ACCESSION   DADWTH010000398 DADWTH010000000
VERSION     DADWTH010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.guided.275"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            112..207
                     /locus_tag="JNY02_004531"
     CDS             112..207
                     /locus_tag="JNY02_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBB9435724.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="JNY02_004532"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="JNY02_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBB9435725.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1015..1185
                     /locus_tag="JNY02_004533"
     CDS             1015..1185
                     /locus_tag="JNY02_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBB9435726.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
BASE COUNT          663 a          303 c          297 g          554 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atggtgt
//
LOCUS       DADWTH010000399         1704 bp    DNA     linear   BCT 16-APR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS369531
            SAMEA2247134-rid11412443.guided.283, whole genome shotgun sequence.
ACCESSION   DADWTH010000399 DADWTH010000000
VERSION     DADWTH010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2247134
            Sequence Read Archive: ERR449061
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941340.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 05:42:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,535
            CDSs (total)                      :: 4,456
            Genes (coding)                    :: 4,033
            CDSs (with protein)               :: 4,033
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 423
            CDSs (without protein)            :: 423
            Pseudo Genes (ambiguous residues) :: 0 of 423
            Pseudo Genes (frameshifted)       :: 207 of 423
            Pseudo Genes (incomplete)         :: 117 of 423
            Pseudo Genes (internal stop)      :: 140 of 423
            Pseudo Genes (multiple problems)  :: 39 of 423
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2247134-rid11412443.guided.283"
                     /strain="ERS369531"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..474
                     /locus_tag="JNY02_004534"
     CDS             <1..474
                     /locus_tag="JNY02_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB9435727.1"
                     /translation="DDKFITTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRD
                     TADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGR
                     QEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="JNY02_004535"
     CDS             657..1517
                     /locus_tag="JNY02_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBB9435728.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          455 a          374 c          437 g          438 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc
     1681 gctgatgtcc ggcggtgctt ttgc
//
